LOCUS       DAJSBL010000001       154091 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.001, whole genome shotgun sequence.
ACCESSION   DAJSBL010000001 DAJSBL010000000
VERSION     DAJSBL010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 154091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 154091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..154091
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.001"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(26..178)
                     /locus_tag="ONT85_000001"
     CDS             complement(26..178)
                     /locus_tag="ONT85_000001"
                     /inference="COORDINATES: protein motif:HMM:NF016526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HCR2377008.1"
                     /translation="MRIFTGGVHLNSNILCYFMSVVLVIVAYQCMFIFRNVDFIILGL
                     MFEFFW"
     gene            complement(369..1247)
                     /locus_tag="ONT85_000002"
     CDS             complement(369..1247)
                     /locus_tag="ONT85_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HCR2377009.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            complement(1286..2404)
                     /locus_tag="ONT85_000003"
     CDS             complement(1286..2404)
                     /locus_tag="ONT85_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HCR2377010.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            complement(2401..4500)
                     /locus_tag="ONT85_000004"
     CDS             complement(2401..4500)
                     /locus_tag="ONT85_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HCR2377011.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            complement(4551..5372)
                     /locus_tag="ONT85_000005"
     CDS             complement(4551..5372)
                     /locus_tag="ONT85_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HCR2377012.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            complement(5369..6178)
                     /locus_tag="ONT85_000006"
     CDS             complement(5369..6178)
                     /locus_tag="ONT85_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCR2377013.1"
                     /translation="MYDAFVVALAVFIGIAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            complement(6199..6693)
                     /locus_tag="ONT85_000007"
     CDS             complement(6199..6693)
                     /locus_tag="ONT85_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCR2377014.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            complement(6705..7193)
                     /locus_tag="ONT85_000008"
     CDS             complement(6705..7193)
                     /locus_tag="ONT85_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCR2377015.1"
                     /translation="MFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLSSIQL
                     LTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITKTFLE
                     KEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVNEEED
                     FF"
     gene            complement(7270..9966)
                     /locus_tag="ONT85_000009"
     CDS             complement(7270..9966)
                     /locus_tag="ONT85_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HCR2377016.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
     gene            complement(10394..11467)
                     /locus_tag="ONT85_000010"
     CDS             complement(10394..11467)
                     /locus_tag="ONT85_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR2377017.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            complement(11464..12291)
                     /locus_tag="ONT85_000011"
     CDS             complement(11464..12291)
                     /locus_tag="ONT85_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR2377018.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            complement(12292..13098)
                     /locus_tag="ONT85_000012"
     CDS             complement(12292..13098)
                     /locus_tag="ONT85_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR2377019.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            complement(13110..14195)
                     /locus_tag="ONT85_000013"
     CDS             complement(13110..14195)
                     /locus_tag="ONT85_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR2377020.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            complement(14212..14754)
                     /locus_tag="ONT85_000014"
     CDS             complement(14212..14754)
                     /locus_tag="ONT85_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR2377021.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            15012..16001
                     /locus_tag="ONT85_000015"
     CDS             15012..16001
                     /locus_tag="ONT85_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCR2377022.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            15998..16735
                     /locus_tag="ONT85_000016"
     CDS             15998..16735
                     /locus_tag="ONT85_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HCR2377023.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            16732..17283
                     /gene="coaC"
                     /locus_tag="ONT85_000017"
     CDS             16732..17283
                     /gene="coaC"
                     /locus_tag="ONT85_000017"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HCR2377024.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            17299..17931
                     /locus_tag="ONT85_000018"
     CDS             17299..17931
                     /locus_tag="ONT85_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HCR2377025.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            18016..18663
                     /locus_tag="ONT85_000019"
     CDS             18016..18663
                     /locus_tag="ONT85_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR2377026.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            18668..19651
                     /locus_tag="ONT85_000020"
     CDS             18668..19651
                     /locus_tag="ONT85_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HCR2377027.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(20054..21568)
                     /locus_tag="ONT85_000021"
     CDS             complement(20054..21568)
                     /locus_tag="ONT85_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HCR2377028.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
     gene            21706..22512
                     /locus_tag="ONT85_000022"
     CDS             21706..22512
                     /locus_tag="ONT85_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2377029.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            complement(22687..23256)
                     /locus_tag="ONT85_000023"
     CDS             complement(22687..23256)
                     /locus_tag="ONT85_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR2377030.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(23339..25399)
                     /locus_tag="ONT85_000024"
     CDS             complement(23339..25399)
                     /locus_tag="ONT85_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HCR2377031.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRREESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            complement(25676..27154)
                     /locus_tag="ONT85_000025"
     CDS             complement(25676..27154)
                     /locus_tag="ONT85_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR2377032.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
     gene            complement(27452..28387)
                     /gene="murB"
                     /locus_tag="ONT85_000026"
     CDS             complement(27452..28387)
                     /gene="murB"
                     /locus_tag="ONT85_000026"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HCR2377033.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            complement(28460..29380)
                     /locus_tag="ONT85_000027"
     CDS             complement(28460..29380)
                     /locus_tag="ONT85_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR2377034.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQEADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            29493..30245
                     /gene="xth"
                     /locus_tag="ONT85_000028"
     CDS             29493..30245
                     /gene="xth"
                     /locus_tag="ONT85_000028"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HCR2377035.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            31109..31642
                     /locus_tag="ONT85_000029"
     CDS             31109..31642
                     /locus_tag="ONT85_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HCR2377036.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
     gene            complement(32132..32656)
                     /locus_tag="ONT85_000030"
     CDS             complement(32132..32656)
                     /locus_tag="ONT85_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR2377037.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(32649..33122)
                     /gene="tsaE"
                     /locus_tag="ONT85_000031"
     CDS             complement(32649..33122)
                     /gene="tsaE"
                     /locus_tag="ONT85_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HCR2377038.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(33245..34225)
                     /gene="pta"
                     /locus_tag="ONT85_000032"
     CDS             complement(33245..34225)
                     /gene="pta"
                     /locus_tag="ONT85_000032"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HCR2377039.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(34378..35058)
                     /locus_tag="ONT85_000033"
     CDS             complement(34378..35058)
                     /locus_tag="ONT85_000033"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HCR2377040.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            35184..36053
                     /locus_tag="ONT85_000034"
     CDS             35184..36053
                     /locus_tag="ONT85_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCR2377041.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            36079..36594
                     /locus_tag="ONT85_000035"
     CDS             36079..36594
                     /locus_tag="ONT85_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2377042.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(36641..37234)
                     /locus_tag="ONT85_000036"
     CDS             complement(36641..37234)
                     /locus_tag="ONT85_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR2377043.1"
                     /translation="MLEWRKGEETMIRFARKEDAPEIAPLILVILKDMELPFLKQTGE
                     QKTLEILEEAITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKY
                     QLDASTRLFIDKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLS
                     VDEKNPKAKKLYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(37259..37438)
                     /locus_tag="ONT85_000037"
     CDS             complement(37259..37438)
                     /locus_tag="ONT85_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2377044.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(37515..38024)
                     /locus_tag="ONT85_000038"
     CDS             complement(37515..38024)
                     /locus_tag="ONT85_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2377045.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(38120..38500)
                     /locus_tag="ONT85_000039"
     CDS             complement(38120..38500)
                     /locus_tag="ONT85_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCR2377046.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            38830..39579
                     /locus_tag="ONT85_000040"
     CDS             38830..39579
                     /locus_tag="ONT85_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HCR2377047.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            complement(39763..41040)
                     /locus_tag="ONT85_000041"
     CDS             complement(39763..41040)
                     /locus_tag="ONT85_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2377048.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(41119..41763)
                     /gene="pcp"
                     /locus_tag="ONT85_000042"
     CDS             complement(41119..41763)
                     /gene="pcp"
                     /locus_tag="ONT85_000042"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HCR2377049.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            complement(41962..42906)
                     /locus_tag="ONT85_000043"
     CDS             complement(41962..42906)
                     /locus_tag="ONT85_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HCR2377050.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            complement(42908..43594)
                     /locus_tag="ONT85_000044"
     CDS             complement(42908..43594)
                     /locus_tag="ONT85_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HCR2377051.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGIASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            44068..44388
                     /locus_tag="ONT85_000045"
     CDS             44068..44388
                     /locus_tag="ONT85_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HCR2377052.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            44542..45447
                     /locus_tag="ONT85_000046"
     CDS             44542..45447
                     /locus_tag="ONT85_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR2377053.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            45444..46217
                     /locus_tag="ONT85_000047"
     CDS             45444..46217
                     /locus_tag="ONT85_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HCR2377054.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(46398..47822)
                     /locus_tag="ONT85_000048"
     CDS             complement(46398..47822)
                     /locus_tag="ONT85_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR2377055.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            48033..48479
                     /gene="nrdI"
                     /locus_tag="ONT85_000049"
     CDS             48033..48479
                     /gene="nrdI"
                     /locus_tag="ONT85_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HCR2377056.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            complement(48544..50526)
                     /locus_tag="ONT85_000050"
     CDS             complement(48544..50526)
                     /locus_tag="ONT85_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCR2377057.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(50540..51436)
                     /gene="murQ"
                     /locus_tag="ONT85_000051"
     CDS             complement(50540..51436)
                     /gene="murQ"
                     /locus_tag="ONT85_000051"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HCR2377058.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            complement(51439..52500)
                     /locus_tag="ONT85_000052"
     CDS             complement(51439..52500)
                     /locus_tag="ONT85_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HCR2377059.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            complement(52711..53277)
                     /locus_tag="ONT85_000053"
     CDS             complement(52711..53277)
                     /locus_tag="ONT85_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HCR2377060.1"
                     /translation="MVKGNFANEGENHMLYVTRHGETTWNAQGLVCGRADVPLTEKGQ
                     MQAQKLAEKVVDLPVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYD
                     GLPSKDENFQKARLAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIR
                     VINAYFHPMPNEGFFTFMVDNTELVSYE"
     gene            complement(53305..54807)
                     /gene="eat(A)"
                     /locus_tag="ONT85_000054"
     CDS             complement(53305..54807)
                     /gene="eat(A)"
                     /locus_tag="ONT85_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HCR2377061.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            complement(55065..56186)
                     /locus_tag="ONT85_000055"
     CDS             complement(55065..56186)
                     /locus_tag="ONT85_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HCR2377062.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            56312..57253
                     /locus_tag="ONT85_000056"
     CDS             56312..57253
                     /locus_tag="ONT85_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HCR2377063.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            complement(57403..58299)
                     /locus_tag="ONT85_000057"
     CDS             complement(57403..58299)
                     /locus_tag="ONT85_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HCR2377064.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            58568..59908
                     /locus_tag="ONT85_000058"
     CDS             58568..59908
                     /locus_tag="ONT85_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR2377065.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            60126..60569
                     /locus_tag="ONT85_000059"
     CDS             60126..60569
                     /locus_tag="ONT85_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HCR2377066.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(61073..62491)
                     /gene="nhaC"
                     /locus_tag="ONT85_000060"
     CDS             complement(61073..62491)
                     /gene="nhaC"
                     /locus_tag="ONT85_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HCR2377067.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(62894..64117)
                     /locus_tag="ONT85_000061"
     CDS             complement(62894..64117)
                     /locus_tag="ONT85_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HCR2377068.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(64148..64987)
                     /locus_tag="ONT85_000062"
     CDS             complement(64148..64987)
                     /locus_tag="ONT85_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HCR2377069.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(65004..65684)
                     /locus_tag="ONT85_000063"
     CDS             complement(65004..65684)
                     /locus_tag="ONT85_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR2377070.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(65686..66759)
                     /locus_tag="ONT85_000064"
     CDS             complement(65686..66759)
                     /locus_tag="ONT85_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR2377071.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     misc_binding    complement(66815..67053)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    complement(67096..67329)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(67406..68077)
                     /locus_tag="ONT85_000065"
     CDS             complement(67406..68077)
                     /locus_tag="ONT85_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HCR2377072.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(68164..70842)
                     /gene="mgtA"
                     /locus_tag="ONT85_000066"
     CDS             complement(68164..70842)
                     /gene="mgtA"
                     /locus_tag="ONT85_000066"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HCR2377073.1"
                     /translation="MSFGHKGEIEMEKKKDTQKIYAAFAGKTKQELFELFHASANGLT
                     EEQIERSRENYGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPG
                     DKDLFGVAIIFTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEE
                     IVCGDLVRLSAGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSY
                     ENLVFMGSNVISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKF
                     MSLMAPAVIVINGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGT
                     VIKNLNAIQNFGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQT
                     GLKNLLDVAIIDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGA
                     IEEMLNISKFIDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFS
                     VKDESDMVLIGYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLE
                     INELVTGEKISEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGIN
                     DAPAMKVADIGISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKAT
                     ASSNFGNMFSVLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKW
                     NASSIGEFMVYFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAG
                     WFVESLWTQTLVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLM
                     PLPGNYWLWLFATVIAYLALVTAVKKIYIRRFQELL"
     gene            71501..71938
                     /gene="ndk"
                     /locus_tag="ONT85_000067"
     CDS             71501..71938
                     /gene="ndk"
                     /locus_tag="ONT85_000067"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HCR2377074.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(71960..72826)
                     /locus_tag="ONT85_000068"
     CDS             complement(71960..72826)
                     /locus_tag="ONT85_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR2377075.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(72986..75079)
                     /locus_tag="ONT85_000069"
     CDS             complement(72986..75079)
                     /locus_tag="ONT85_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HCR2377076.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
     gene            complement(75828..76451)
                     /locus_tag="ONT85_000070"
     CDS             complement(75828..76451)
                     /locus_tag="ONT85_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2377077.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
     gene            complement(76610..78007)
                     /locus_tag="ONT85_000071"
     CDS             complement(76610..78007)
                     /locus_tag="ONT85_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HCR2377078.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            complement(78039..79010)
                     /locus_tag="ONT85_000072"
     CDS             complement(78039..79010)
                     /locus_tag="ONT85_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR2377079.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            79384..79809
                     /locus_tag="ONT85_000073"
     CDS             79384..79809
                     /locus_tag="ONT85_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2377080.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
     gene            complement(80297..80998)
                     /locus_tag="ONT85_000074"
     CDS             complement(80297..80998)
                     /locus_tag="ONT85_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HCR2377081.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(81014..81454)
                     /locus_tag="ONT85_000075"
     CDS             complement(81014..81454)
                     /locus_tag="ONT85_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HCR2377082.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            complement(81454..82029)
                     /locus_tag="ONT85_000076"
     CDS             complement(81454..82029)
                     /locus_tag="ONT85_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HCR2377083.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
     gene            complement(82877..83356)
                     /locus_tag="ONT85_000077"
                     /pseudo
     CDS             complement(82877..83356)
                     /locus_tag="ONT85_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339057.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            complement(83515..84216)
                     /locus_tag="ONT85_000078"
     CDS             complement(83515..84216)
                     /locus_tag="ONT85_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR2377084.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            complement(84324..85733)
                     /locus_tag="ONT85_000079"
     CDS             complement(84324..85733)
                     /locus_tag="ONT85_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HCR2377085.1"
                     /translation="MEQLLPNQLEGAYDRDGKGLSVADAMPGGKQRFAIIGSEEFDWT
                     IDQEKYIYPNHRGIDHYDRFKEDLALFAEMGFKCYRFSIAWTRIFPNGDEGTPNEAGL
                     EFYDQLIDECLKYDIEPVITISHYEMPLHLAKEYGGWKNRKLIDFYERFAQTVLERYS
                     SKVKYWMTFNEINSAFHFPALSQGLVKSNGAGEYQNIFQAWHNQFVASSKAVKIGHEL
                     RSDIQIGCMIIYATTYSIDANPVNQAATMIQNQEFNFFCTDVQVRGEYPAYTARTHKK
                     YGVDPEKLEQTEKDFQLLKEYPVDYIGFSYYMSTAINETDPQAATSEGNLLGGVKNPF
                     LEASEWGWQIDPEGLRIALNELYNRYQKPLFIVENGLGAIDHMEEDGTIIDDYRIDYL
                     RRHIEAMANAVADGVDLMGYTPWGCIDLVSASTGEMSKRYGFIYVDLDDEGNGTLNRS
                     KKKSFDWYKEVIASNGVNL"
     gene            complement(85781..87646)
                     /locus_tag="ONT85_000080"
     CDS             complement(85781..87646)
                     /locus_tag="ONT85_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCR2377086.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGKPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            complement(87767..88618)
                     /locus_tag="ONT85_000081"
     CDS             complement(87767..88618)
                     /locus_tag="ONT85_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCR2377087.1"
                     /translation="MKIDKILNNNVVISKNGFGEEVVCMGKGLAFQKKIGDEISPEAV
                     QKEFVLRDSFAKNQFQQLMADIPAEEIEWVKQVVELAEDKLKVEFSPNIYLTLTDHLH
                     YAITRAKENIQLPNPLLFETKKFYPKEYQAAKEALLFVKQKTGVELSEDEAGFIAFHF
                     VNSQQGNEDMQVTMTATTMVRDILSIISKFFGIVFDEESLNYQRIITHLQFFAQRYLK
                     GEGSDEQDEFLYALVQGKYPKAFHCVERINEYLRQTQKQEMGIAEQIYLTIHIQRVVS
                     EKKSIQS"
     gene            complement(88928..89641)
                     /locus_tag="ONT85_000082"
     CDS             complement(88928..89641)
                     /locus_tag="ONT85_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HCR2377088.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
     gene            complement(89709..90035)
                     /locus_tag="ONT85_000083"
     CDS             complement(89709..90035)
                     /locus_tag="ONT85_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HCR2377089.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            complement(90158..90385)
                     /locus_tag="ONT85_000084"
     CDS             complement(90158..90385)
                     /locus_tag="ONT85_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003246265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HCR2377090.1"
                     /translation="MLTQQLRELEQDESIHRKAYNQVPPKVEYSLTEEGKSLREILVA
                     LSVWEEERIEKQKASREEDTLLHTHGGYLNY"
     gene            complement(90707..91960)
                     /locus_tag="ONT85_000085"
     CDS             complement(90707..91960)
                     /locus_tag="ONT85_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HCR2377091.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGCIALMVVTLL
                     LPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGSRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            92021..92761
                     /locus_tag="ONT85_000086"
     CDS             92021..92761
                     /locus_tag="ONT85_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR2377092.1"
                     /translation="MKKYFIIEDNQFHSEWIIRELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            complement(92847..93605)
                     /locus_tag="ONT85_000087"
     CDS             complement(92847..93605)
                     /locus_tag="ONT85_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HCR2377093.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            complement(93602..94630)
                     /locus_tag="ONT85_000088"
                     /pseudo
     CDS             complement(93602..94630)
                     /locus_tag="ONT85_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628085.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
     gene            complement(94650..97817)
                     /locus_tag="ONT85_000089"
     CDS             complement(94650..97817)
                     /locus_tag="ONT85_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2377094.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTNESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQNFKGTAAHPGGLTPNIDGNAIIIAYMTDFWGSRYHFTGEIKFTGTLDLYD
                     GSKLGLIYAPRATVTLDGVSGVLDVQPVKEGLNAAPGKAYFCRSYQLNVINGSQLYGP
                     YLGKFYGFLDSGDETGSGKTAPGIHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVMF
                     EVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSKVTVSNGGKITINTATTGGFRLQGD
                     SSQIHVSSGGQISVKMAGDDDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSAV
                     RFENGTQKLTVTNGGSLRVRNTGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFTV
                     TGEKSNIDIRADSGATVDTTGDIDLNFTAGEKTYLVMVGKTGEDKTGIISGDVLNVDL
                     ESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKTGATFDNFEKDPD
                     YFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRVP
                     TNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSSVEITALTQGQTDEN
                     EGAGYNPWGEGEQGGLFEVQVPNDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTVD
                     VIPPEPANLSTTLINAASRTITGTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPEN
                     QLFAGDVLEIVLNDQAGEATAKGVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATKI
                     VVEGGLSFLAPSKLDFGQIKATGLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLSQ
                     PFTNDAGFSMQDVLYFKKGEEYQLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKLV
                     LTKENQIAGNYSAEITWTLADAPTE"
     gene            complement(98041..98925)
                     /locus_tag="ONT85_000090"
     CDS             complement(98041..98925)
                     /locus_tag="ONT85_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HCR2377095.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            complement(99020..99457)
                     /locus_tag="ONT85_000091"
     CDS             complement(99020..99457)
                     /locus_tag="ONT85_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2377096.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWMFYLVKK"
     gene            complement(99674..100585)
                     /locus_tag="ONT85_000092"
     CDS             complement(99674..100585)
                     /locus_tag="ONT85_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCR2377097.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
     gene            complement(101042..101470)
                     /locus_tag="ONT85_000093"
     CDS             complement(101042..101470)
                     /locus_tag="ONT85_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2377098.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
     gene            complement(101498..102136)
                     /locus_tag="ONT85_000094"
     CDS             complement(101498..102136)
                     /locus_tag="ONT85_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR2377099.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            complement(102140..104122)
                     /locus_tag="ONT85_000095"
     CDS             complement(102140..104122)
                     /locus_tag="ONT85_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HCR2377100.1"
                     /translation="MSGFALVSINKQVLYRTNEFFHQTDTYTPIRLPQHFIGQEEYEQ
                     LIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLEMTLYYQGDVKSSHEKVPY
                     SNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERFISLFTDRFNQAFHTDYATNIFE
                     DFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLLMNKEISLLLNNDFSLHQV
                     LWCLLGKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYVFLFSGIYGISLISLLLVE
                     KIASLREAFNYIQYILFTFGLLFSLLLTVANVELATIVFSSSQLAVSHEPPKEIAGED
                     YHVFYPVTIGKNHVDFIYSNRFASAADQELYETLNKNGSILVNVSDYLNEENEQFGRG
                     IKINLNYLKKFPLIDVSGRLIQITEKETHPVILIPERYRHTDLKNDLAQITEYYQEIS
                     EKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSMYLERNILSGEIYPPLKIK
                     IGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLSRSFHYLIQIFLLTFFSFF
                     LLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNLIKWGTTSVIFLFLIEREP
                     KYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            104302..104967
                     /locus_tag="ONT85_000096"
     CDS             104302..104967
                     /locus_tag="ONT85_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR2377101.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
     gene            complement(106902..110051)
                     /locus_tag="ONT85_000097"
     CDS             complement(106902..110051)
                     /locus_tag="ONT85_000097"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HsdR family type I site-specific
                     deoxyribonuclease"
                     /protein_id="HCR2377102.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIEREDSQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIVQIELK
                     QVTAKDGVYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNEKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGISSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSSEQINRMIDQMEPVKKESYLDPAVFEADEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKEPDWLTKEFPNQLIREGRTM
                     EDPDFPRIAITYSLEENSRDSSAQQEEMQKIIEEYNQYYDTSWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLAIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PDKTKGLIVTFRKPATMEKNVEDATKLYSKAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVKSPTLNNQIQLLEE
                     QIGVYETVKGSLIEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSD
                     AREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDDLIHV
                     FSNEWFVSEKELHASNLQYLPGEDPIPNMKAIINSKDYEGYKAKHPEAKPFKYPQEMK
                     RAWRKMLDDELIPLENELR"
     gene            complement(110064..111281)
                     /locus_tag="ONT85_000098"
     CDS             complement(110064..111281)
                     /locus_tag="ONT85_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HCR2377103.1"
                     /translation="MSNDTQPEIRFPGFTDDWEQRKLGDFVVDYVEKTSVQNQFPMLT
                     SSQQKGIVLQEDYFANRQVTTENNIGYFVLPRGYFTFRSRSDNDVFVFNRNDIIDRGI
                     ISYFYPVFTLKSADSDFFLRRINNGIQRQLSIQAEGTGQHVLSLKKFKNIVAMFPSEE
                     EQQKIGTFFKQLDDTIALHQRKLDLLKETKKGFLQKMFPENGAKVPEIRFPGFTEDWE
                     ERKLGELGKTQSGIGFPDAEQGGSEGTPFFKVSDMNNIGNEYEMRNANHYVSNEQIER
                     KKWKPIKDVPAIIFAKVGAAIMLNRKRLVTSPFLIDNNTMAYLFNNTWDIYFGKILFE
                     TINLPRYSQVGALPSYNSSDIENISVKVPVKDEQQKIGTFFKQLDDTIALHQRKLDLL
                     KETKKSFLQKMFV"
     gene            complement(111271..112866)
                     /locus_tag="ONT85_000099"
     CDS             complement(111271..112866)
                     /locus_tag="ONT85_000099"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HCR2377104.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAIAPEYLFNVLA
                     DQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKK
                     LNDVDVLEHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERR
                     FFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMN
                     LRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADF
                     AFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGT
                     SIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYAERKDVEKYA
                     HLATFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKKLLEAIS
                     SLQTTPENEAWLQGALEVFKHEQ"
     gene            complement(113376..114158)
                     /locus_tag="ONT85_000100"
     CDS             complement(113376..114158)
                     /locus_tag="ONT85_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2377105.1"
                     /translation="MENSSNSAEVNVSTSTSEVVKCSQETTSLPEELLGDWQINNKDA
                     GIEISDSKAVFRIGRSQVESKDYYVDVNNKMLLHVYFSFEYDSVKYIEVSIERTGYSE
                     FISKSVGILTMHTKDGQYISLGVKRVSGENNETEAVTEQTMDERIDSSKSNETEVDTG
                     KTIDERIDSFIETYTAIPPTWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNA
                     VTEQVGKDIPLRLLENGKKDYSVVFKNGQVTDYNEVFKKVIE"
     gene            complement(114223..115059)
                     /locus_tag="ONT85_000101"
     CDS             complement(114223..115059)
                     /locus_tag="ONT85_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCR2377106.1"
                     /translation="MRVIKTLNNSLILCLDKQGKEVILMGKGIGFQLKKGDLVPADKI
                     QKVFKLDSLSDALKKDYIKLFEAAPDDLIICVKRIIDYANAKFDFKLNKSLFFTLVDH
                     LKHAIERSQKGIVFQNRLLIEIQKYYPKEFEIGCYAIQLINKKMNVQLPEEEAGNIAF
                     HIVNAQSEQANMEETMLSVRALKDILAMLGYLFPEKKVDKDSMLYLRFVTHLQFFIAR
                     MIKKEELPEKDDFLLASVKQRFPKEYRAALKIKEYVQEEMGASANQDELLYLTLHISR
                     VY"
     gene            complement(115077..116486)
                     /locus_tag="ONT85_000102"
     CDS             complement(115077..116486)
                     /locus_tag="ONT85_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HCR2377107.1"
                     /translation="MQYKQLSAFPEKFLWGASISAYQAEGAYQEDGKQPSIIDKYQHT
                     EGVANFEVASDFYHHYKEDIQLFKELGLKAFRFSIAWTRIMDIENNKTNQKGISFYHQ
                     VIDECLNQGIEPIVTMYHFDLPYFLEEQGGWLNRATIDYFVDYVQVLLTEYGDKVNYW
                     LTINEQNTMILHPGAIGLPPSGELPSKKELFQINHHVLLAQARVIHLYHQLNLKGKIG
                     PAINLTAMYQATSSPEDAIAAHNWETLRGWSFLDVAVRGKYNYLFENYLNDRGLYPKI
                     EAEDQAILSSGKPDFIAINYYSTATIAASKNDGSDVSARAGDQQIMLGEEGVYRAAEN
                     PYVDKTPYGWVVDPTGLRLTLRKLYDRYDLPILITENGYGAPDIVEEDRRINDQDRID
                     YLAKHILAIQAALTDGVDVFGYLPWSAIDVVSTHQGFNKRYGFIYVDRTDENLKELKR
                     IKKESFFWYQKVIARNGLA"
     gene            complement(116500..118323)
                     /locus_tag="ONT85_000103"
     CDS             complement(116500..118323)
                     /locus_tag="ONT85_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCR2377108.1"
                     /translation="MDYREVGRNVLQQVGGKENVVSLTHCATRLRFELKDKRKADTKA
                     LEKTLGVISVVDSGGQYQVVIGNEVQTAFKEIQKEVGTSIKKGPSSDNQESIISRLIS
                     VISTTFTPMIPAITGAGMVKAILAVLTLTGVLSDSSQTYIILNTIADAAFYFMPVLLA
                     YGAAIKFDVNPILAITVAGILLHPNISQLFSTGDAVSFLGIPVLAADYAGSVLPIIFT
                     VWIMSYVEPFADRISPSFIKFFTKPMLVFLIMGPLALVVIGPFGTLLNDLIAMGAEWI
                     NGKAAWLIPFLMGGLQPFLVVTGTAWAMTPIATMQLSSTGSEMINGPGMLASNIAQSG
                     ATFAVAAKTKNKELKQLAVSTGITALMGITEPSLYGVTLKLKKPLVASMIGGAIAGLY
                     AGLSGLVRYAFVSPGLAALPAFIGENPMNIVHAIITCVIAFVATFIIAYLIGFDDTSD
                     ESEQTAIAEAVIVENPVKGTVCPISEVNDAVFSKGLLGKGVAIHPEENSIYSPVEGEV
                     ITILESKHAIGLRSKEGIELLIHVGIDTVNLKGEPFEVFVKAGDQVSAGQLLINADFA
                     AIRNAGYDDTVIVVVTNSENYAGISEASGSLKEHEPLMQIS"
     gene            complement(119166..119492)
                     /locus_tag="ONT85_000104"
     CDS             complement(119166..119492)
                     /locus_tag="ONT85_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2377109.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKAILDNGSAININIEYYPKKDRIYVEIPYA
                     EVLMLN"
     gene            complement(119538..119900)
                     /locus_tag="ONT85_000105"
     CDS             complement(119538..119900)
                     /locus_tag="ONT85_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HCR2377110.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEKMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            complement(119937..120152)
                     /locus_tag="ONT85_000106"
     CDS             complement(119937..120152)
                     /locus_tag="ONT85_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR2377111.1"
                     /translation="MKKVSNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPMNELFSDVFNKVNS"
     gene            120279..120614
                     /locus_tag="ONT85_000107"
     CDS             120279..120614
                     /locus_tag="ONT85_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR2377112.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(120690..122687)
                     /locus_tag="ONT85_000108"
     CDS             complement(120690..122687)
                     /locus_tag="ONT85_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2377113.1"
                     /translation="METSMINIPIYVDQEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSVEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFNHKRSNVYRLDVFNATSVIQKLNEDILPLLEKIHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELINTVC
                     ERLRNVGDNSVKNALIKNRGYFVKQGFTLERASKDIKKLYNVKASSSFLIYLAKGAGI
                     DETIKELILSGAGIVPLYQFTSVTRDDGRRVRKRKDPIVLN"
     gene            complement(122785..123060)
                     /locus_tag="ONT85_000109"
     CDS             complement(122785..123060)
                     /locus_tag="ONT85_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR2377114.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            123292..124026
                     /locus_tag="ONT85_000110"
     CDS             123292..124026
                     /locus_tag="ONT85_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR2377115.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            124065..125207
                     /locus_tag="ONT85_000111"
     CDS             124065..125207
                     /locus_tag="ONT85_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR2377116.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(125302..126693)
                     /gene="sufB"
                     /locus_tag="ONT85_000112"
     CDS             complement(125302..126693)
                     /gene="sufB"
                     /locus_tag="ONT85_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HCR2377117.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(126698..127168)
                     /locus_tag="ONT85_000113"
     CDS             complement(126698..127168)
                     /locus_tag="ONT85_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HCR2377118.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            complement(127155..128390)
                     /locus_tag="ONT85_000114"
     CDS             complement(127155..128390)
                     /locus_tag="ONT85_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HCR2377119.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            complement(128387..129673)
                     /gene="sufD"
                     /locus_tag="ONT85_000115"
     CDS             complement(128387..129673)
                     /gene="sufD"
                     /locus_tag="ONT85_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HCR2377120.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            complement(129686..130459)
                     /gene="sufC"
                     /locus_tag="ONT85_000116"
     CDS             complement(129686..130459)
                     /gene="sufC"
                     /locus_tag="ONT85_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HCR2377121.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            complement(130656..131504)
                     /locus_tag="ONT85_000117"
     CDS             complement(130656..131504)
                     /locus_tag="ONT85_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HCR2377122.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            complement(131528..132214)
                     /locus_tag="ONT85_000118"
     CDS             complement(131528..132214)
                     /locus_tag="ONT85_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR2377123.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            complement(132207..133241)
                     /locus_tag="ONT85_000119"
     CDS             complement(132207..133241)
                     /locus_tag="ONT85_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR2377124.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     misc_binding    complement(133304..133560)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(133636..133974)
                     /locus_tag="ONT85_000120"
     CDS             complement(133636..133974)
                     /locus_tag="ONT85_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HCR2377125.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     gene            complement(133976..134332)
                     /locus_tag="ONT85_000121"
     CDS             complement(133976..134332)
                     /locus_tag="ONT85_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HCR2377126.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            complement(134338..135525)
                     /locus_tag="ONT85_000122"
     CDS             complement(134338..135525)
                     /locus_tag="ONT85_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HCR2377127.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            complement(135716..136561)
                     /locus_tag="ONT85_000123"
     CDS             complement(135716..136561)
                     /locus_tag="ONT85_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR2377128.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            136795..137760
                     /locus_tag="ONT85_000124"
     CDS             136795..137760
                     /locus_tag="ONT85_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HCR2377129.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            138137..139180
                     /locus_tag="ONT85_000125"
     CDS             138137..139180
                     /locus_tag="ONT85_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HCR2377130.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            complement(139242..139706)
                     /locus_tag="ONT85_000126"
     CDS             complement(139242..139706)
                     /locus_tag="ONT85_000126"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HCR2377131.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            139952..140899
                     /locus_tag="ONT85_000127"
     CDS             139952..140899
                     /locus_tag="ONT85_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HCR2377132.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            complement(141110..143182)
                     /locus_tag="ONT85_000128"
     CDS             complement(141110..143182)
                     /locus_tag="ONT85_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HCR2377133.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(143115..143492)
                     /locus_tag="ONT85_000129"
     CDS             complement(143115..143492)
                     /locus_tag="ONT85_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HCR2377134.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            complement(143621..144199)
                     /locus_tag="ONT85_000130"
     CDS             complement(143621..144199)
                     /locus_tag="ONT85_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCR2377135.1"
                     /translation="MRGNMEMKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDI
                     KAPSLYNHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSN
                     QAVYELLISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYL
                     SLRFLGYFTKEPTIDPEDSYFWMINNFIATLP"
     gene            144331..145623
                     /locus_tag="ONT85_000131"
     CDS             144331..145623
                     /locus_tag="ONT85_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HCR2377136.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            complement(145683..146801)
                     /locus_tag="ONT85_000132"
     CDS             complement(145683..146801)
                     /locus_tag="ONT85_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HCR2377137.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(146815..148236)
                     /gene="arcD"
                     /locus_tag="ONT85_000133"
     CDS             complement(146815..148236)
                     /gene="arcD"
                     /locus_tag="ONT85_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HCR2377138.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            complement(148482..149603)
                     /gene="mnmA"
                     /locus_tag="ONT85_000134"
     CDS             complement(148482..149603)
                     /gene="mnmA"
                     /locus_tag="ONT85_000134"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HCR2377139.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            150007..150606
                     /locus_tag="ONT85_000135"
     CDS             150007..150606
                     /locus_tag="ONT85_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HCR2377140.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            complement(150744..152030)
                     /gene="efmA"
                     /locus_tag="ONT85_000136"
     CDS             complement(150744..152030)
                     /gene="efmA"
                     /locus_tag="ONT85_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HCR2377141.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLTGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(152301..152645)
                     /locus_tag="ONT85_000137"
     CDS             complement(152301..152645)
                     /locus_tag="ONT85_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HCR2377142.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            complement(152782..153927)
                     /locus_tag="ONT85_000138"
     CDS             complement(152782..153927)
                     /locus_tag="ONT85_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HCR2377143.1"
                     /translation="MNEVKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGR
                     HAHEKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIE
                     HPAVLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLM
                     PIKEIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKR
                     DGVNIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLIL
                     DTLEANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNI
                     EPSHVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
BASE COUNT        44841 a        32101 c        26488 g        50661 t
ORIGIN      
        1 tattttatac ggctgaaaaa atctatcacc aaaaaaattc aaacattaag ccaaggatta
       61 tgaagtcaac atttcgaaaa atgaacatac attggtaagc aactataact aataccacag
      121 acataaagta acacaatata ttactattta agtgtacgcc accagtgaat atcctcaagg
      181 tatacagcgc acaacaaaaa gaaaaaagga aacgcagaag catttccttt cagtgattta
      241 atgaaaacaa cacgatttga cttagagcct ttttatttga attcattttc gctttatcca
      301 acttcatttg atcatttgat tactcggtta aatacttttg acatgagtat caatgaaaca
      361 ttcattaatt atggttgctt atttcttacc gcttttctgg ccaatgctaa tccgccgatt
      421 attccggcat tatcacctaa ttcgggcgta ataatgtagc tatcaattgg tggtatttca
      481 acatatccat ttataagctc aacaaattta tcctgaactt ttttcttcat gtgttcttgt
      541 ttcattacac caccgccaaa aataattcta tctggcgaaa acatcagtgt tgtattgtat
      601 gcacattggg caaggtaaaa ggcttcaatc tgccaaaacg aatcgtcagc caataaattc
      661 tggcctttta cacccaaccg tttttcaatc gctggtcctg ctgccatccc ctctaaacaa
      721 tcttggtgga agggacagtt tcctgaaaaa ttgtcctttg gatgtcgctt gatcaacata
      781 tgccccattt ccggatgact gaacccttcc acgaaagtcc cattgaacaa ggcaccacct
      841 ccgatacctg ttcccacagt gaagtatgca acacctgcta accctttgcc agaaccagca
      901 acatattcac catagcaagc cgcattgaca tctgttgtcc attcaatcgg aatattgaaa
      961 tattgcttta gcgttcctac aaaattgaaa ttttgccaag caagttttgg tgttgatgtg
     1021 atatgaccga aactcgttga tttgcattga atatcaattg gaccaaagca gccgaccccg
     1081 atgcctgcca attgttcctt aaaaggttta aaaaaatcaa tgacctgctt cattgtttca
     1141 actggagttg ttgtgggaaa actgactctt tccaaaatcg ttaattcctc agtaccgact
     1201 gcacagacaa atttagtccc tccagcttca attgccccat atatcatcat aggttcctcc
     1261 cttcgaatat gtgaaaaaca cttcattaac gcggcaagcg ttctcgatca gtgaaatgac
     1321 gtttagtatt tcctgaagct tctgttgaat accagtaagc gacactagca acatcgtctt
     1381 gccgctcaaa taagcgaaca tcatcgttgc cgatttgttg taaagtgacc gataaatctt
     1441 cggtaaaaca gatggggtct tgaagatgcc aacgataaag accatgtcgt ggcattgcgt
     1501 cgtcaccaaa ggcatgaacg gcattcaatt ttcccgtttc aaaacgatct cttgtggcat
     1561 ctctatttga ttggtaagga taccctaaaa aggctgttga atagtttttc gcatgtggac
     1621 gtccgaactc atcgtactca tgaaaggccc acgccccgcc aaaataatct tctgaacctg
     1681 ttgaggttac ggtcggatac tcactatccc catcaatgta gaatttaaac tcgccttcgc
     1741 cccaccaata acgttctagg caggtaagcg caaggtaagt acccacgtaa tagccttgac
     1801 cctttaatcg atcaattaac ttataatcct cctgtaattt ggtaatgcgt tcccgatgcc
     1861 aatttgcgtg aaaatatagg ctattttctt ccaattcgtc caccaaagcg taatttatcg
     1921 tataaaaaaa tgaggacata tccttcggat gtaaattctc gatcgtaatt ttagcggatg
     1981 tttggaaagg catttcgaaa taggaattaa agccccctgt aggattaacc actatcggca
     2041 acgagttcac ctcgcaacgc tctccaaacc cattacaaaa gaaatcccct aaaggacttt
     2101 caacagcagg gctatccgac ccatcccaat aaattcttaa gattaaatca cgtaaaacaa
     2161 aactgccaaa ttctgttcga tcacgaatcg tcatccacat atgacgaatt attcccgttc
     2221 cagtaatatc cgcaatcaca gtttcttcac cagaagtcag cggaattgcg ccacgacctt
     2281 tgcggcttgg tcctagctcg cttggcaaca tcgcagcttt acctttctcg cctgttggat
     2341 tttcgggagt gattgctcga ctctggattt ttttaaattg ggtaatattt tttagtatac
     2401 tcatcttttt ctcctttgaa tgtgaatcgt ttttcccttt tctacgtgaa gaattttttt
     2461 cgtatcactt attttcaatg gcttttctga caaatttgaa atactcaact cggattgcgt
     2521 acattcaatc gaaatgaagg cttgaccgaa cttcaaattc ttccaagaaa acgttaaatt
     2581 atcaacagga tcaatttgca atactccttt tttgaatttc aatccgaaat attgactgat
     2641 cattaggctg acaaaaagac ccgatgccca gccgcatttg ccgcagccaa agtctcgcac
     2701 gacttctcct tgttgagcct ctaagcgata cggccaccac caccacgaac cgtctaaatc
     2761 tgccaaacaa attagttctt ctaatcgggt tacgaaagct tgagggttgt gcagctcgct
     2821 cattaaaaca gtgataaagc caggaaaagt ggcgccggat tctaatcccc aagtgatgcc
     2881 tttgatttcc tcgctatagg ttggattgaa agaactagca gaaaaggcca tggtgttctg
     2941 atataaatga tctttattat ctaaaaattg ataaaaaggc atcaacgtag tgtctgattc
     3001 ttctccatca tgcatcaata aattaatttt tccatccaca atcacatcac ttaaaaagat
     3061 taacccttgc tcaagaatcg gtttttgata gtgcttcact gaataggttg ttttctcctc
     3121 atcaccgatt ccctccaaaa attgctcacc aaaaggacca tcgatagtca tttgtttaca
     3181 aattgattcc tttagtttac aggcaatgtt tttgtatcgc ttgctaagac tcagttgttc
     3241 tagggcttca aaaatttctg caagtccaat acaggccttc caaagacaaa gattgctgcc
     3301 ggtatgatac tttccaagtg caaaggcgtc tgataaccaa acagatggaa aaagcattgg
     3361 ttcccctgac cgacgttgag ctaatatgtc ctcaataatt cttgaagcat tcagtagaac
     3421 ctgcggacac cgctcaaaga attttgtatc cccagtatgt tcaaaataaa gactagccag
     3481 taacgcactg gataacgaat tactcagaga atgattgata cctcctgtaa attttgaacc
     3541 aggaaactta acgccatatt gatcaaacca tagaaccgtt ttttgacata actccggttc
     3601 aaacatggtg aatggcaatg atgcgtaata catatcctta ttccaaattc gattagtagc
     3661 aggatagctg ccccaattgc tgccgacgac ctgtccctgt cgattcatac cgaaactaga
     3721 aaagctttga taaagtgcac gacgatagag atggacaact ctatcgtttg gcattgacag
     3781 ctgcccaaat agctgagcaa aatagttcaa tgtttccttc agccattttt cgatatcaga
     3841 ttcctgaaaa gtcattactt cttcaaaagc atttgggtcc acaaaagcaa tggcgaagct
     3901 tgccacttcc tgttgtgcga tcgtgtcctt atttcctttt tgagaaatca acacattttg
     3961 ctgatccgaa tacttctctt gatacgaagg cacatctttc actgaaactt gaaggctttc
     4021 gtgtgtttca ttcacaatgt aaacctgttg aacaagcata cttaaacgct tattagccag
     4081 tatgggagca aaggatacta caatgacctt aaacgtcgaa taatgatgaa tatatctagg
     4141 caaacagtct tgaatcacat cgacttcaat acgatgatcc gattctgaca aatggtagac
     4201 aatcccgttt acttcaacct ttaaggataa cgaattggtt tgagagagct gtttattgac
     4261 ccaaacacct ggtttttcct cagttagtaa attttctcga taaaaggtca acgtttgaat
     4321 cgtcccgcaa acatcaaggt ttgctaatgt cttttgatta cttacatcga aggtcaatcc
     4381 ctttacatca ggaatattcc gatagtagct gtattcgttt gaagtagtca catttccgtc
     4441 gtttaactca gcattaaaaa atgggtgttt gggtgaataa attacttcat taatagtcat
     4501 cttttccctc cttgctaaaa aaactgcagc ctagtagctg cagcttccta ctatgcgatt
     4561 ccaagtaatg acaataaaat tcccaagacg accattccaa aaataatttt gatgacgccg
     4621 acttttttat ctagtaattt gaaacagctc aatgttacca ataacggtac aattccttta
     4681 aaaatactat cgaggatttc ttggacattc attagttctt cgcccttcat tgaaaagctc
     4741 aaaacggtat tgaatttgac catactgatc gtcattgcac ctaccatgat cagacccaag
     4801 atactagcag cttttgtcag aatttcaatt aacccgttcg catacagctt ttcaatatat
     4861 ttagagccta atgaatagcc taaaacgccg ccatagtatc gcatcaagta attaggtata
     4921 ttgaacataa ctaaaaagat aattggtgcc aaaatatttc cggcattggc taagttgatc
     4981 gctagtcccg cggtaacgac acgccaaact ccccagaaca atgaatcgcc aattccagcc
     5041 aatggtccca tcaaactcgt tttgatcgcg ttgatacttt ccacgtcaaa accagctttt
     5101 tcagaatttt ccttctccat cgatgccgca atccccatga caaaggacgc gcaaggaatg
     5161 gtcgtcgaaa aatactgtgt atgacgttcc agcgccgcag ctcgatcttc ttcacttttg
     5221 tagaaatgat taatgaaggg catcatagaa taggcgaaac caccgccgcc catcgtcacc
     5281 ggattcaccg aagcatacat agtcatcgag cgccaaaaca tcttccgaac caattttttc
     5341 tcatctttag aaattccatc agctaatttc attgaaagaa atcctcctct tcttggtcaa
     5401 gcgttgcatt tacagcagca tcgctttgaa ctgcatgaac agatatattt tttagttgta
     5461 gatctctttg ggctataaca attgcaataa tcgttccaac tgcggcaatt gcaatcaatg
     5521 gtaactcgag atacgccata acaacggccc caaagaaaaa ccacacagca atatttttac
     5581 tccaaagcat ccgtaacagc atcgccatcc caactgccgg cagcagattt ccagcgactg
     5641 ttaatccgtt catgatgacc tctggaatat tatccaagac gcttttgaca acatctgctc
     5701 caaataaaac ggcaaagaat ggaataagag cataaagcgc atagtggaca aaccacataa
     5761 agaagtgcag attgatgatt cctttttgat caccagattt tgcaaatttg aaaaatttag
     5821 cagccatcgg tccacataca aaagaaaaaa tcatattcat taactgtaat cctaatactg
     5881 ctagtggaaa gacaatggga ataatcgcat ccacgtttcc tcccattttg attgcgaacg
     5941 ctgtagccag cccagcagcc aaagaaggct ccgccgtact agagattccg atattgacga
     6001 cacccataaa gatggcttct agtgacgccc cgacaatcaa ccccgtctgc aaatccccca
     6061 tcaacgctcc tgctaacgga gcaacaacaa tcggacgact cagcatggac atcccgaaca
     6121 tttcatgtcc agcaattcca ataaaaacgg ccaaagcgac tacaaatgca tcatacataa
     6181 tttttctcct tcctttttct aaaaatcact caaaatagag atagctgaac gtttttccat
     6241 cgccggtgta ggctgaaaaa cggtatctgg ataaagttca actaattctt ttaagtcttg
     6301 aatttctgac gtcgttaaca tcacttcctt tgataaggtt ttcttatctt ccgatggcgg
     6361 tgtcattttc ccagcattcc ccacgttcat taattcgatt tccgttggga aagctttcgc
     6421 aacttttacc gcatctttga cattagggac taaaaccatc aagcgcatgt ccttcgcccg
     6481 cggatctttt aacaaatgaa tcgcgtcatc aactgtttta ataatgactt tgacattcga
     6541 tggtgcagcc atcttcaatg tcatcttttg aatatcgttg gtagccgcct catcgcttgc
     6601 tactactaac ccggttaaat ttagactctt tgtccaagtc atcgctactt gtccatgaat
     6661 taatcgttca tcaattcgca ctgctttaat catttttctt cctcctaaaa aaaatcttcc
     6721 tcttcattta ctacatgtac ctcatcaaga atggtcatct ttaattcctg ttggcattta
     6781 tttaccagtt cctgaatatc ctcctttgtt aattccttgg ttgccaatac tatttcgagc
     6841 agaattggca gattaaagcc agacaaaata gttacagcaa tctctttctc caaaaaggtt
     6901 ttggtaatct tctgattgac acttccgcca aataaatcgg taaaaacaaa aatctgatcc
     6961 tgctcgttca cctggtttac aaatacattc aactcctgtt caaaatcaca ctcatccaca
     7021 taagcattaa tcaccgtaat ctcttgaacc ttccccgtca gtaattgaat actgctaaga
     7081 aagcctgaag ctaagtgacc gtgagtcgct attagaattt tcttcatatg gttaattcct
     7141 ttctttctgt tcacttttat ataagcaatt cctgtgccaa cttttaaaaa cattggttta
     7201 atcacgttaa ttcacattaa aaaaagtgtc taacctaaaa aaaacagatt agacactttt
     7261 ttagacactt tatttttgaa tatacggtcc taataattca tagagcagcg aaagctcata
     7321 agcattgact ttaaaattat atttcatctc cattgattga aaaatacttc tagaaacata
     7381 ataaaattca tcttcatctg gtttcaaatc ataattcggt tcttcagatt tttctgtccg
     7441 agccaagaat aaacgttcca tcatcaatgc cgtatgcata tagagattta gttttacacg
     7501 gccatttaaa gtgaaatgat agtaattttc atatttagaa atgactgttt ccacttcact
     7561 catcacgatc gttgggttca gaaagttcaa acggtccgca attccttcca acgaaaagaa
     7621 ttttactagc tcctgaatca agcggttaaa ttctgccggc ttcatttcag gactcaaaat
     7681 atgctccaaa ttggctgctc catcaccgtc taagatgtca taaacattaa tattaggaat
     7741 tgaaaaggaa cgtggaagat ccgaagtcgt gatgaccaat tttgtcttgt caaaataagt
     7801 ttcatcattt tggtcgatta attcacgtag ttctctgtag tccttcgtga aaacttcgat
     7861 ggattcatcc gataaatgat gacgaaaaac atctttcaat ttgtccgaaa tccccaaacc
     7921 cgacatacag gaaatgataa tgttgctact ttgagaaaag ccttcaaagt attgaacatt
     7981 gattgaatac ttgtctgctg catcctccgc aatctgttta aatggcgcat tcatcgtcat
     8041 cttcaagcca atatcaagtg caatggaagt cgtcaaatta ttcagaagaa gtaactcccc
     8101 ttttaaatcg ttcttgatct gtgtgtaaat ttgatttaaa gagcccatat ctacgatcag
     8161 cactagacta tctgtattca cttgccgttt cacgaaattc cttgtcgatt cgacaatctc
     8221 tgacacatta ctctctactg gcatatccaa cgcttcaaaa acataattgc cgcataattg
     8281 attcactact gcttgaatgc tgctagccgt actatctccg tgagcaagca acaggccctt
     8341 cagctcgatg gattcatcta cataatcaga taaagtcacg acaataagca gtcttagaaa
     8401 gttctcgtcc ttttctgaga gcggtggtaa atgctccaag aactgattac ataagtaagt
     8461 cgtccgaggt agatttgcag ctaatgtacg atttatcccg gttaagtctg tcttatagct
     8521 tggggaaatg acataggatc tgctaataat ttctgcaaca ggtccactcg tcgtcaatcc
     8581 gtacgttttt cctaaaacgg atacccagac ctttctaaac atctgaatca tcaattttct
     8641 actcattgat aaaaaagaat ccttatcaat gtgatgcacc aaatgctgta cttcattctt
     8701 tatcgaagaa aaattttcat tgaaattacc ttcatttatc agctgagtaa ttttttccaa
     8761 aatcgaatca taatcaaacg gtttgattcg atccgttttt atcttttgat ttttctttat
     8821 caataaacta gcaatcggca agctttctac ttcggattcc agcatattta actgaatcaa
     8881 gatctcattt ttcccaatcg ttaatggttc ttcttgtgtt cgactataca catccgcaca
     8941 actgatttta atgatattct tcagcttacc aatattccca ctaaagggat gagataacat
     9001 taaagacaag gcttctcttg aaacaataat atctttttgt aaaacatccg cttcattttg
     9061 aaaaaataat tgaatcagct ctaatcgttc tcgaatgggg cgatcttcaa aagttggcaa
     9121 cataacacta atttgaatac gccgatcaaa ggtgtctaat aaataatttt cacctttttc
     9181 cgtcgtggcg aaaataaaac gaaccttagc agaatgccaa tttacatttt caccgactgg
     9241 acgatagttt ccagtatcaa taaatagaaa gagcttttct tgattttctt tcgataggcg
     9301 atggacctca tcgagaaaaa gatagccgtt gttggccgtt gccaataaac catcggtatc
     9361 cttttcagcc cccgtaaagg cccccttctt gtatccaaac agtgttgccg aaagcagttc
     9421 gggattgtcc gcataatcgg cacagtttaa gatagcaaat ggtgcatctt tttcaataat
     9481 ttgctcatgg atagcgtatt caaaaatttt tgtggctaaa aaactttttc caacacccgt
     9541 ggctccattt attaatacac tgagtccgtt aggcggatat ttgacagcgg ctatacattt
     9601 ttgaatgacc tctctcagac ttccgtcatg accaactaca gaatgaaagg cttcgtttat
     9661 ttgctccgta ttatctacaa ccgcatattc atctaaaggc ttccacaaaa caggctttcc
     9721 cggtaatttc tcgacctttt tatcttccac taatctcgtc aaataatggc tggcattctg
     9781 acgagagata cctagattct tcgaaatttt ctcggcagtg acagaagaca tattccctgt
     9841 tagtcgtatc agctcatcaa aaactcgatc tttagcgctc tttttcattt tttcctccag
     9901 tttaatgtgt ctaaaagcat aataaactat cggctgcaat atgtctattc tatatttgta
     9961 ttccaatttt tactttgaaa cacaaatagt cgaatatttg cctcagatat ctgacaaaat
    10021 aatttatcta ccaatttagt ttttatctaa tatgattttt atgttttttc atagctacaa
    10081 atattgaact ggattcatat attagtttta ttaatagatt acagaagggt taagtatatt
    10141 tgcatagttt ccaattactt tatccttctg taactttttc aatcttagat ttactagccc
    10201 gtctcgggcc ctattttaca atggttttgt ccacttttct ctggtagaaa aacgtggtag
    10261 aaaaaataaa ataacacaaa aaaagcggcg tttcatccgc ttttggtaga agattggaaa
    10321 actcagatag aaaaaggacg atttttagct ctttagaacg ttgataagtc cccattctta
    10381 ctgagttttg ccattatctc ctatacatct tgaattctaa gaataggtca ttatagatgc
    10441 ctaggtattt cgatcctaga tctttgtaga cttcaagatg ggagatcgtg tcatcagatg
    10501 gatagaattg ttcgtccgac gaaatgtctt ttggcaataa cgcttttgct tcttcatttg
    10561 gtgtagaata accaatatac tctgcatttt gtgccgcatt ttccggttct agcataaagt
    10621 tgatgaaatc ataggcacct tctttatttt tcacggtttt tggcataacg atattatcaa
    10681 accaaagatt ggatccttct ggtgggatca cataatgtaa atgttcattt tcccacatca
    10741 tatcggcagc ttcgccagaa aatgtcactg ccacggaggc ttcttcattg atcatataca
    10801 ttttgatctc atctgccacg attgctttga catttggtgt taaatgattc aatttatttg
    10861 ctgcttctgt caactgtgcc atattcttac tattcaaaga atagcctaaa ctattcaaag
    10921 aaagccccat aacttctcgt gcaccgtcaa tcagcatcaa actgtctttt aattccggct
    10981 tccacaaatc atcccaatgc ttaatttgat ttgcatcaaa gaatttatcg ttatatacga
    11041 tccctaaagt tccccaaaaa tatggaatcg aatactgatt atctggatca aaatccaagt
    11101 ctaaaaatcg tggatcaata ttatttaatc ctttgatctt cgaatgatct aatggaagta
    11161 ctagtttttc tttgatcatt ttttggatca tatattcact tggtatagcg atgtcgtagt
    11221 ttgtcccacc ttgctggatt tttgtgaaca tcgcttcatt agaatcaaat gtttcataat
    11281 tcactttgta tccatattct ttttcaaatt tcgtgatcaa actaggatcg atataatctc
    11341 cccagttata gatggtaagt acctttgctc cactcatgcc acttgttttt tcaagttgac
    11401 gaacaccaag cagcagcacc gcaataatga tgagaatacc taaaaacaga gattgtaatt
    11461 ttttcatcgc agccgtgcca ccccctcgcg tactcttctg tttttcttca gccgttttgg
    11521 acggttttct ttactaataa agtagtatcc aatgaccaat atcatggaga acaagaaaac
    11581 taacgcactt agcgcattga tttctaaact gattccttgt cttgcacgag aatagatctc
    11641 tactgagagc gtcgtaaagc catttcctgt gacgaaaaat gtaaccgcaa aatcatcaag
    11701 agaataggta aatgccataa aatatccagc tatgattcct ggagaaagat aaggcaaaat
    11761 gatatttttg actacttgga aattattagc accaaggtct ctagccgcat cgaccattga
    11821 atcgttcatt tcctgcaatt ttggcaagac catcaagacg acgattggaa tgctgaaagc
    11881 aatatgggaa agcaatacac tctgaaaacc taatccgaat cctagtagtg taaagaaaat
    11941 caagaaacta gcaccgataa taacatcagg agagactaat aagatgttat tcatgctcaa
    12001 caacgctgtc cgtgcacgtc gttttttcgt gtaatagatt cccatagccc caaatgtccc
    12061 gatgatcgtg gcgatcaatg cagataagaa agccagcatg aacgtattca atacgataat
    12121 gatcaaacgt gtatcttcaa aaacagcttc ataattttcc caagtaaatc ctgtaaagct
    12181 gttcatcgtt cctccttcat tgaaggaata aaaaatcaga tagaagatcg gtgcatataa
    12241 gaataaaaag acgatcccta aataaagata cgaccattta aattttttca tttatgagcg
    12301 acctcctttt ttcttctcgc ctgtcaggag catcacgatg aacatagcaa tgattaaaat
    12361 cacaccgatt gtagaaccca tcccccagtt ttgtgtaacc aagaaatgct gttcgatagc
    12421 cgttcctaaa gtgatcaccc gatttccgcc aatcagacga gtcagcatga ataaagaaag
    12481 agaaggaata aatactgctt ggacaccact tttcacacca tttaatgata acggaaaaat
    12541 cactcgtcgg aaggtctcaa agctattcgc gcctaaatct ctactagcac tgatcaaaga
    12601 aggattcatc tcttccaatg cattgaaaat tggcataatc ataaatggaa tctcgatata
    12661 cgttgccacg aacagaaaac tgaagtctgt gaataatatc tgtttcggcc cgatcccgat
    12721 aaaatcaaag aaatgattca cgctgccgtg cacactgaaa atcccaataa atgcgtaagc
    12781 tttaagcagc agattcaccc atgtaggcaa aataatcagc atcagccata attgcttgtg
    12841 cttcagtttt gttaaaaaat aagcagtcgg gtaactgatg atcagcgtga aaaacgtgat
    12901 caaaaatgca taccatacag aattgatcgt catagaaagc gatgtccccg acgtaaaata
    12961 agatagataa ttattcagcg tgaattgccc attcatatca aaaaaagatt gataaatgat
    13021 cattaataca ggcgcgatca cgaataaacc gagccacaaa atataaggga cggaatagat
    13081 cctgcgcatt gttttcattc ttgtcccccc tattcttcgt agctgtcaag acgtgcatcg
    13141 aattcttctt cagactcgtt gaaacgcatg acatggatat cttctggctc aaaggccaat
    13201 cctactttgc ttccttcttt agcttttttc gttgaatgga ccatccattc attttgctgt
    13261 tcatcataac agatgatttc ataatggaca ccacgaaaca gctgagtatc gaccgttacg
    13321 actagttttc ctttatctgg tgtcgtgatc gtcaaatcct ctgggcgtaa aacaacttca
    13381 actggttcat ttagacgcat cccgccatcg acacattcaa attgcttacc aacaaactca
    13441 actagattat cttcgatcat tacaccgtcc acgatattgc tctccccaac aaaatcagca
    13501 acgaaatgat tgattggctc atcataaata tctacgggtg taccactttg gacgattttc
    13561 cctttattca tgacaaaaat ctcatcactc attgccaacg cttcttcttg gtcatgagta
    13621 acaaaaataa acgtgattcc taatcgttgt tgcagatccc gcagttcata ctgcatatct
    13681 gtacgtagtt tcaaatcaag cgccgataac ggttcatcta atagcaatac tttcggttca
    13741 tttacaattg ctcgtgcaat cgctacacgc tgtctttgac caccagacat ttcactaatc
    13801 tcccgttttt cgtatccagg aagctggacc atacgcaaag catcttttac cttcttttcg
    13861 atatctgatt tggacatttt cttgatcttc aatccaaaag caacgttatc aaaaacattc
    13921 atatgaggaa aaagtgcata atcttggaag actgtattta cttgtcgctt attagctggc
    13981 aaatcattca accgtttccc ttcaaaatag atgtcccctt ctgtagcttc ggtgaatcca
    14041 gctataatac gtaggatcgt tgttttacca cagccggaag gaccaagcag tgtataaaat
    14101 tttccttgtt cgatctcaaa acttactttt tttaatactg tttcatcatc atagcgtttc
    14161 actacttgat caaatgaaat gattggtttc cccacgtttt taccccctat attacaaata
    14221 tgattgcgtt gcaacaatca atacttttgt tcgttttttg agatgattcc ttaattgatg
    14281 ttgttttgtc gcttgataat agatcgactg tcctgctttt gccacataga ccgtctctcc
    14341 taagcttaac tctaattccc cttctaatac aaaaataaac gtttcagata atgaaggttc
    14401 aaacgtctta tattctcctg caccttcaaa agtcagcaag acaggctcca tttctttttc
    14461 attcgaatca gccacgagcc attccaattc atacccgttt tcttcatcga aataaaccgt
    14521 atgatcttct tttgaataaa ccacttgcag attcgctgct tcttgatgaa aaaattcttc
    14581 tggagtcaca cctaagactt caagaataga aaagaatgtt tccattgatg gtgaacttaa
    14641 atctcgttcc aactgggaaa tatacccttt tgacaaatct gtgcgttcgc ctagttcttc
    14701 ttgtgtcaaa ttcttttgga tgcgcaaatt tcttaacttc tcaccaattt ccataatttc
    14761 ctccgaatcg aagttttcta atagtaaact tcctgtttac taccactcgt attagtatac
    14821 gaaatttatt gaaaaaggca accctttgta aaattttttt tgctgaatat ttctttatct
    14881 tttggaataa attctagttg tttgattctt tatttttcac ctaatttttg tctaaattta
    14941 cggaagaaca agaataaaag agtaggcttt atggtatgat aaaagaaaat tttaaaagga
    15001 gtgtctcacg aatgaacgct tttcgatctt ttcaggtaat aaaggaacct gttttccata
    15061 cagcaatcag tgatctgcca tttactgagt tggctgaaaa tgaagtcttg gtaaaaattg
    15121 cctattctga tgtcaattat aaagatgcat tggcaatgag cgagtctggt caggtgatac
    15181 gaacatatcc gatgactcca gggattgatt tatccggaac tgtcgtccaa agtaacaatc
    15241 cacgtttttc aaaagaagat cttgtacttg ctactggttt tgggcttggc gtcacccatc
    15301 caggcggtta tagccaatat caaaaagtac caggagactg gttagttcct ctaccaaaaa
    15361 atatgacgct gcgtcaagcg atgatccttg gtacagcagg gtttactgct atgttgtgtg
    15421 tgaacgcttt aatccgtcaa ggtatgacct ccgataaaaa agttgtcgtt actggtgctt
    15481 ctggcggtgt aggcagtaca gcgatagccc tgttacataa actaggattc cagtcaatca
    15541 ttgccttttc cagaaaagaa gagtctgtca catggctaaa aagtctaggt gctttgcaag
    15601 tcgtacgacc tgatgaattt ctgccagaga caacaaaagc tttaggaaaa cagcaaatcg
    15661 actatgtcat cgacacagta ggtggcgagc agctaagcag tcttcttcca ttgatctctt
    15721 ataacggtgc agtagcttta tgtggcaatg ctgggggaat caaactaaac gcgactgtct
    15781 tgccttttat tctgcgaaac atccagttga ttggaattga ttctgtcaat gtaccaatag
    15841 atcaacgtct ttctttatgg caacagatgg cggatttgca gattgcagat gaactggtcg
    15901 tccaagagat tactctggat caattgcctg aaacagcatc taaactactt gctggaacac
    15961 accaaggaag aacactagta aatgtaggtg accataaatg aagattctaa tcacagctgg
    16021 aggaacgact gaaaaaatcg atcaagtacg tgctattaca aatcattcga caggacgatt
    16081 agggcaagca ttagctgatt atttagctgc caatccggat accacaatcg attacgtgac
    16141 tacgaaacaa gcactgaaac cgaaaagaca ctccaatata acgatttata cgatcgaatc
    16201 tgcactggac ttatttttac aattagaaga actaacgaaa aaagaacact acgatgcgat
    16261 tatccacagt atggcagtca gtgattttac tcctgctttt agtttttcag aagaacagct
    16321 ggcaaaaaac ctacccactt cttcaacgca agaagaactg gacaattggt ttgcggaaaa
    16381 tgaacagaca gataccactg tttcaaagat ctcttctaac accgagcatc ttgtgctcgt
    16441 tttgaagaaa acaccaaaaa tcatttctta tctacgtgaa tggcagccaa aagcaaaaat
    16501 cattggtttc aaactattag tagatgtacc aaaggaatct cttctggcag tcgcaaaaaa
    16561 tagtctgata aataacaaaa ctgattttat tttcgcaaat gatttgacgg aaatccatgg
    16621 cgagacacac cacggctatc tcttatctaa agatggaaca gtggaagaag ctcaaagtaa
    16681 atctgagatt gctgcattga ttactgagaa aattagactg gaggaatcaa aatgaaaaag
    16741 atcttactag gcgttactgg aagtatttct gcttacaaaa gcgctgatct tacaagtcaa
    16801 ttcgtcaaat taggtgccca ggtagatgtg attatgtcta aaagcagcac acattttatc
    16861 acgccattaa ccttgcaatc tctgtcgaag cgcgcagtcc atacagacgt catgcaagaa
    16921 tcaaatccgc aggtaatcaa tcatatcgaa ctagcaaaac aagctgactt gtttctcatc
    16981 gcgcctgcta cagcaaacat tatcggcaag ctagcaaacg gcattgcaga cgatcttctt
    17041 tcaacagtcg caatggcgct tccagtggga acaccaaaat tgattgcccc tgccatgaac
    17101 acgaatatgt atcaaaaccc gatcactcag cgtaatctgg aaacattgag atctgtcggc
    17161 tatcaggaaa ttgaaccaag agaatctctt ttagcttgtg gagattttgg aaaaggtgcc
    17221 ttagctgata ttcagacgat catcgaacag gtcatgctga ctcttaatga aagaaatgct
    17281 tagaaaaaag gagtacttat gaaaaataca aaaaacttta cattaactgc gatgttttta
    17341 gctattttga ttttattagc tgttacgcct ttaggtttta tccctatcgg tccaatcaat
    17401 gcaacaacca tgcatattcc tgtaatcatt gcttcgatcg ttttaggacc aagactaggt
    17461 gcttttcttg gcggtacatt tggactgatc agcatgatcc gtagcacagt tatccaaaca
    17521 cctttgtctt ttgtcttttc tccgtttatc ccagtcatcg gaacagaaca tggaagctgg
    17581 aaagccttgc tgatcgcgtt tattcctcgc atcttgattg gggtggttcc ttatttcgtt
    17641 tataaaggtc tgcgcaaact aatgaaacaa aaagcagatt ccgtttcttt attcttagct
    17701 ggactttccg gttcattgac caatacgatt ttagtgatga atatgatcta ctttttattc
    17761 cagcaagatt atgccaaagt catcggaaca aatttaaatg ctgtttattc agctattcta
    17821 gcggttattt ttacaagtgg tatacctgaa ggaatcgtcg caggtcttgc gactgcagca
    17881 gtatgcaatg tcttgttaag atacttcaag cacccttctc ttactaactg atagtcatcg
    17941 tagccgatct ctgatttttt tctcttttgg tgttactatt gaatggaaag atttgacgtc
    18001 gaaaggatga tccaaatgaa aatttttgtt gtaggtgcaa acggacaaat tggccgacac
    18061 ctgataaaag atttagctcc aagttcccat gaaatttttg caggggtgag agatgttgct
    18121 tcccaaacat tagtaaaaaa agagaatgtt tcgtatgtct cttttgattt aacttggtcc
    18181 gtggaaaaaa tgaccgaagc attcaaaggc atcgatgtat tgatttttgc tgccggctct
    18241 caaggaaaga atcttctgca agtagacttg gatggcgcaa tcaaaacagt gatcgcagct
    18301 gaaaatgcgc atgtctctcg ctatctgatg gtcagtgctg tttatgcaga tgaaccagca
    18361 aaatggcctg agtccatgac agattattat attactaaac attatgcaga tgaatggctg
    18421 aaacgaacta acttagattt tgtcattctt caaccagtca cattgactaa cgatgaagaa
    18481 gttacttcca ttcaattaac taagcctaat gagaaggcat caaaaacgat cactcgctct
    18541 acagttgctg ctgtacttgc cgctctagtg gaagaaacgg atatttctcg tacgactcta
    18601 gtactttctg aaggtagcaa agagttgaat acagcattcc aagaatgggc aaaggaggaa
    18661 tagatccatg cgtgccattt caatcaagca tcctggttct gtagatgtat tagaacaaat
    18721 tgaagtgcct gttcctaaac caaaacaagg cgaattactg atcaaagtcc atacagcagc
    18781 tgtcaatcga acagatatta tgaaaagaga atcagtctct ttgaaacctc cctaccctat
    18841 tttgggagtc gaagtagctg gagaggtagt tgagaataaa agtgatatct ccacttttac
    18901 tcctgggaca aaagtatacg gattagtcaa tctaggtggt tatgccgaat atgctgtgat
    18961 gcctgccgat cgtgcaatcc ttctccctga tacgctagac tatgtttctg ctgctggaat
    19021 ctcagaagtt tttttgactg cttatcaaac actttactgg cttggaaaat tacaagaagg
    19081 cgaaactgtc ttgatccatg caggagcaag tggtgtggga acagcagcga tccaattagc
    19141 tagacagctt tctcacgcga aaatcatcgt tactgcagga tcagaagaaa aactcgcctt
    19201 ttgtaaagaa cttggtgcag atgaattgat caactataaa aaacaagact ttcaagaaga
    19261 ggtacgaaaa atcactgaag gtcggggagt cgatgttatt ttggatttca tcggcgcttc
    19321 ttactgggaa aagaatttag cttctatagc cgttgatgga cgttgggtct tgattggtat
    19381 gctaggtggg accatcgtgc ctgagataga ccttggtgta ttgattggga aacgtgtcca
    19441 actgcttggt acattattga cacctagaag tgatcagtat aaagcagagc tcgcacaaac
    19501 ttttatgcaa aaagctgggc cttttctaga aaatggcaag attcggccaa tcattgaccg
    19561 tacattccaa ttcagcgcag tcaaacaagc gcatgaatat atggaagcaa acaaaaacat
    19621 cggaaaaatc attttgaaga taactgaata aaatcatgct gatcaaaaaa gaggctggga
    19681 cagaagcgtt taactccaag aaataagaag aaattcacga aaattgcttt tcaaattttt
    19741 gtgaatttca gcttatttcc gaaggaattt cttctgcttc cgccgtttat acgtgtttag
    19801 agcgtgagac aaaagtgttc tttacttttg tcccacgctc tttttccgaa taaacggtgt
    19861 tcaaaagctt gcatctgcgg taataagtcc aaccaagtta aaaacataag aagctgtttt
    19921 tacttggctg ttcttattac ttgatgcagg gcagcttttt cacaacctct tcactaaaca
    19981 gtgttcaatc agctgaccct cggtattagc tcaaaacgct gatttcacaa cctcttttta
    20041 tttacgttct atttcattca ttttccgaag caaatcgagc tgcttgtatg cccgcttgtc
    20101 gcccaaaaat aatgatctcc gcaactgaat ttccaccgat ccggttgttt ccatgtaagc
    20161 cacctgtgac ttcaccagca gcatataacc ccctaattgg ctggttttct ttatctaata
    20221 cttcagtgtt cgtattgatt ttcacaccgc ccatcgtgta atggattcct ggtgcgattt
    20281 tgatcgcata ataattcgga gacgataagt catgttccat ggctgtggca cggttgaatt
    20341 gagcatcttt tttctccgca acatccgtat tccatccatc aactgttttt gctagtgttt
    20401 cttttggtaa atcgattttt tctgctaatt cttccattgt ctttccagtt gtgacatatc
    20461 ctttttggtc ataaaattca atagctgtgg cacgatcacg cacgccttgg tcaaaaatca
    20521 aataagctga tttttcaggc aatgctgtaa ttgctgcaga tactttatcc cttgtatcca
    20581 tttcattgac aaatcttttc ccctcttgat cgaccaagat tgctccttct ccgcgaacaa
    20641 cctcaccaat caagaccccc tcatcctgct gcacagttgg atgaatttgt attttatcca
    20701 tatcaacggt ttgtccacct aatttttcaa tcatggtgat accatctcca gttgatcctt
    20761 cttgatttgt cgtaatataa tcagctaaat cgggacgata tttttcaata aattctttgc
    20821 tggcaccaaa accgcctgtg gtaactacga ttgcttttcc ggtgacctct tttggttctt
    20881 ctccttgaac atgtacagtc acttcattga ccgtttcccc atttttctta atgtctgtca
    20941 catctgcgtt gacaaaaata gggatttccc gctcatggac gttattcaat aacccctcca
    21001 ctaagtatcc accaatagcc gagccatcag acggacgatg tgtcctcttt tcactcatcc
    21061 ctccagtgat cgttaagttg tccagtttga taccgttttg atctagccaa tcgaccgcat
    21121 cagcagagtg atcgacaaag tatcgaagca tttctttatc attcgttcct ccaccacctt
    21181 ttagcgtttc ttcatagaag gcatcattcg agtctttgat tccttcttct ttttgaaatt
    21241 ttgtttctga agcattcata ccgcttgatg atttagatgt gtttcctccg gcaacaggca
    21301 tcttttccaa aattactgga ttcaacccag catcttttgc ttcgatagct gctgccatcc
    21361 ctgcaccacc agctccaata atcacgacat cataagattc tttcatttct tttggatctg
    21421 tatatacttg ttcagaagcg cctgatgata cttcggcaga ttccttcgta ctagaacttg
    21481 tctgcgctgt atttttctga tctgcgccgc atgcgctcat agcaagaagt gcaacggctg
    21541 ttaataaacc agcaactaat tttttcattt gtatccccct tttctcctta tactatatta
    21601 agtgattttt ttcacaatat caactctgtt tttctttaag tcatatagct ttggtaaaca
    21661 cacgcttctt agtataatta aggatataca tgaaggagtt attttatgaa tcatttgaaa
    21721 acctttttta cgaacagtac aaaaactgca ggtagaatca caaacggaat ctatatttta
    21781 ttacgtatca tcgtcgttat tatgctagtg gatcaggtct ggaaccaaca ctggggaaat
    21841 acaatgcttt tattactgac attaatttta tttgaaatcc cttattttct ggaaaagata
    21901 ttaaagatac agattccaaa tatgctagaa atcattttgt tatcttttat ttttagttca
    21961 acagttttag gagagttaag tgatttttat ggctattttt caatgtggga tacttttcta
    22021 catgcattca gcggattttt agctgggggt gtcggctttt cccttgttta tttgctcaat
    22081 aaaaatacaa aaaccatgaa cttgactcct ttattactag cagttgtttc cttctgtttt
    22141 tcgatgacag ttggtgttat gtgggagttt gtggaatttt cagcagataa attactgcaa
    22201 ttcgatatgc aaaaagatac gatcatccag caaatcaata gtgtggagtt aagtaaagac
    22261 ggaaacactg tctatcatgt tccagatatc actcggacaa tcattgaaag tcaaaatgca
    22321 gatggtaaaa cagataaaac aatcatccaa aatggttact tagacgtggg aatcatggat
    22381 actatgaagg atttatttgt taattttatt ggagcagttg ttttcagtgt atttggttac
    22441 ctctatagtc gaaacgatta ccaaaaattt acgttcatca agaattttat tcctaaaaaa
    22501 atcaaacgat aaaacatgct aaaaaacaag agaaggggca gtgacaatac ttttgtcact
    22561 gccccttctt ttttgaatgt atggcatgcc attagttatc cgatagtatt aactcaaaaa
    22621 agtaagatat gaggaatgac tatttcgttt tttatcccaa tactgaggac ttatttcacc
    22681 atttcgttat ttatctaatt tacttaaaac ctgcataact ttttcataat ctggattagg
    22741 tgtgccgtct agattttcag caataaaagt aaaacccatt tttttataaa gagcaatcgc
    22801 ctgatggctc catgtctgtg tgtgaagata gattggtccg tccgtatgga gggtaggaaa
    22861 ttcttgcaag actctggcag tcaatgcttt tgcgagccct tttccttggt aatctggttt
    22921 cactgctaac caatgaaaaa ccggatacac ttgctctctt gatttttttc gccaagcggt
    22981 acaggttgct actttttctc ctttttttgt ctctacaaaa aacatccttc tttcaagttc
    23041 ttcaggataa ggagcaaagt tttgttgaaa atactcaagt gcttcatctg tcgtttgaaa
    23101 ttccccaacg gaagtctcaa tctccgccca agcagattca tcttcttttt gataaaatcg
    23161 aaattcaaaa ccttccgcta aaggcgtgat tggaacattt tgttgaagtg gtcttgtcat
    23221 ccagatttct gcgtaaggta acgttttatc taacattttt ctatccctcc tcgtctccat
    23281 tatgccaaat aacatatgac tcagctactt atttgactat aacagataga gatataaatc
    23341 agaaccagtc atctgcgtca aattcatttc gattagcgta tacccatgtt cctgccatac
    23401 ctcgcatgaa atctcctaaa gcagaagaag gaatacctcc accaggtgcg ggtgttcccg
    23461 gactaggaag tggtacttgc ggctgcagat gctcatcggt ggaatcaata gttgacaagc
    23521 gttttttctt cgtttccttc tggtcatctg attccttctg atcttccgac tgtttagcaa
    23581 caaattcttt ttctgattgt tgttttattt taggctctaa aatttcattt tccaatccag
    23641 aaacttgtgg ggccaaatga gcttcttgcc ttatcgtttt attagattct gagatttttc
    23701 tagatattct tgctaactct aggcttcttg tttgttttac tttccctgtt ggagaaactg
    23761 catgagccat ttgttgtttt ttttctgaca agctttcttc tcgtcttttt tgttcttttt
    23821 gatctatctg tttgtttaat ggtaaaacat gtaatagtga ctgagcatgg agttcttgga
    23881 gtgaatacgg gtcatgataa ctatacataa tcgtaaaatc acctgtttgt ttggtcaaat
    23941 aaccatcctc caatggtact tcattgattg taaccgatac atcctttaac ggtacgggtt
    24001 tacctaagga atttgttgct gattggatca actcttcttt gtttattttt tcgtttggat
    24061 gaacaataag atcatgacct tctaatgaac gattgtctgg tatataaaca taagtaaagg
    24121 taatttcccc tcctgattgc ttcctattat tcatcagttc tgagtgaagt acttcaagga
    24181 tctccgacca gtgacttttt ctgtctaatc cgtttgcttg catccaatca gaaatagaag
    24241 tgactttccc attttgttca taagtcgata aatctatatt tggcactcga tcagaaaatt
    24301 cagcaggtag gcgcaaatca ggaatacgat ttacactact catatgtgtt tctgcttgaa
    24361 gttctttatt tgtttgacta tctataaaat gagtgatgac agtcgatgaa gcagcagatg
    24421 cttctacata aactacagct gttgtttctg ccacatttga tgttttcgag tctatataag
    24481 ttaatacaac gggatattct cccggagttg tagtatctac tgtcccgttc attttgattt
    24541 cttctttatt tcctgaagtt ccatcgcttt ttgttacata ttcaacaaga tctttagggg
    24601 cgtaactctc tcctgttctt aagtgaagat cttttccttt gatagctgtt tgatcagcac
    24661 gaacatggat cacagcagat gcagtcattg tttttaaagc aattggatca aaataggaca
    24721 aggaaatagt agatgttcct gtattagtcg tatccacgat attcccattg tttgaaatta
    24781 ctttggacat atctccttgt gttccatctg cgttcttcag cctttctacc agatcagata
    24841 tcgaaacttt tgtccctgcc agaacatcga ttttctttct ttcaaactgt gaataatctt
    24901 tttcatatac ataagaaact tgtgcgcttc caccgtcctt tatatctgtc aaagcatgat
    24961 tcaaaatatc agtgatctgt tttaactgat caccttttgt aggatcagtc atatcggaaa
    25021 agatagaaag cggcacactg cttccggttt ccgatgtcca taatgttttg ttaggaatta
    25081 accagtagtg gtttatcgtt ggaggataac tgactttatt ataggaagtc aatgcaggaa
    25141 gtagctgttt tccatttgca tcagtgaaag tatgagtaat agtgataggg gaatcagtgg
    25201 tctcagaaac agaggtacgt acgaccttga tggtttgtgt cgttgcctga acgggtacat
    25261 gcatatagac acctacagat aaactgatca gcatcgtgcg catcataaac ttaacgtagt
    25321 gtttttcaga acacgaccgt tccttatcta aacgacaagc gtgtttattt agttgttttg
    25381 aacgtctttt tgaacacatt tattcttagt cctccctact cttgtctaaa catgaagtag
    25441 taattttcat aagatgattt cattatataa ataaaatcaa aagaagatgg actattagga
    25501 gagtattttt tttgattttt tattgtttca agaaaatcgg aataataact tatgtacttt
    25561 ttaattttgt taagtaatat atctagttat atctagttat atctagttac cttttctttg
    25621 aaaaaaacat aaatcagcta ataaatatgt gagacaaagc tgccatccat aaatactagc
    25681 tgattttttc agttaaatgt ctattttttt ctctgatgat ggtatttcca aaatattccc
    25741 agtcatctgg agtcggtgga tttttccaaa ttatctgttc tttattaaat ttttcaagta
    25801 aacaatcaga taaataaata ttcgtacgag aagtgattcg actctcaatc acaatattgt
    25861 aatttttaaa tcctttcact tgagaaataa tgtaatcggt ataagcagat ccttgcgaaa
    25921 aatcaataca gacatgaatc ggtgcttcaa aatatgatac cggacaaata ccgaccaata
    25981 aaaagagata atcataaaat aagcgaatca tgaaacgttc atcttttccc aagaaatagc
    26041 gtttattctc ttgtatgacc ttttggattg ctgtactgaa tattggatac gtctcgaaga
    26101 aaaactgtag ctgcttttta gacgtaaagc tagtcatctc aaagtcgaat atccaatttt
    26161 ttcgattgac atgcatcata cccatacgaa gttcttttcg tatatatagt ctttcttctt
    26221 cagagatttc aatcgaaaat ggtaattgat caatcaattc cactgacatt ttttgactaa
    26281 tcatgtggat atcatgaaaa tgagcttccg caagtccaaa tgcgccatct aattcaaatt
    26341 cttcttccgt gatcaaaaac aacatgatat aagaaatctc atcaatgatc tgctctttag
    26401 ggatatgaag aaaagaaata tgtgataaat ccttgatgat cttcattcct tctgagctct
    26461 taaagtattc atctgaaaag aaacgttttc cgattggttg attcccatag gccctcaaca
    26521 aactaatagt ggttagcaaa ttgaatttga ttcgttttgt tgcagacagt tttaaattga
    26581 acagatattc aaaatattgc acgatactct ttgcttcatt catgacgctt tctctaaatg
    26641 gcatagaaaa accattaaag accccgaaat ataccccaaa taatacgttc cgtatgttta
    26701 tttcttcccc aactaatatg tttttcttta actttatttg atgctgttca agaaaagaga
    26761 ctaattctct tctttttaca taggctcttg atatgcttaa aaaatgattc tctgcaaatt
    26821 tttcgatcgt tacttttgaa tccacgatct ccgttaacag tcgagcaatt tttgatcgtt
    26881 gaaagtagat tcttcttaaa tttttgatat ccgtcaaatt cagacgttcg acatggatct
    26941 ctgcttcttc tgaaatttgg atctctggct ttgttttgaa ttcagtaaat tctttattta
    27001 attctatgat ataatttctt gttttaaatt tagacaaacc taatgtttca tataacttag
    27061 gcagcactac ataattcttt ttctcaaatt ctaaatatct agctagcagc agtttttctc
    27121 tatcttcctc ttctataagg taatccaact tcatatccta cacctcaaaa aatgcctcat
    27181 accaattact tgatatgcag atgaaagttc atcttttcac ctatactgat tttatgatgg
    27241 attatgcttg ttttttcatc agttattctc attttttatc ttttttttga gaatattgca
    27301 aaaaatggaa aaatttatga ggaaatattc tatatttttt gttttattgt gttaaaatgt
    27361 acaagatttg cgccatttgc gtaaacaacc acaaaaaaga gcaggacggc caccgtcctg
    27421 ctcttttact tattttaagt tccattactt attaattggc taaacttctg cttgtttctg
    27481 atattgcttg tttttctcca atgatccgca cttctgtttc taaagacaca tcaaattttt
    27541 ctttgattac ttcttggatg tgcgcaatca attcaatata atctgtcgct gtcgcatgat
    27601 cgatattgac gatgaatcca gcatgttttt cagagacttg tgcaccgccc catttcaggc
    27661 cttgcaatcc tgcttgttgg atcagctgac ctgtatagtg tcctactggg cgcttaaaga
    27721 cacttccgca tgaagggtat tctaatggct gttttgattg acgcaattcg gtcaactcat
    27781 ccattcttgc tttgattgct tcataatccc ctgtttgcaa actgaatcga gcttccaata
    27841 cgatagcacg taattcttgg actttactgt gacgataaga aaataccatt tcgtcttttg
    27901 tcaaagtttt caatgttcca tcttcaagca aaagatccac ttcagcaaaa acatctttga
    27961 tctctccgcc atacgcacct gcattcatat aaaccgctcc accgacactt cccgggatgc
    28021 cgcaagcaaa ttcaaatcca gtcaatgatt ctgccaaagc tgcataagtc gtatcaatca
    28081 gtgaggcacc tgcttccgca atgaccatcg ttcctttgac atgaatctgt ttcatttcag
    28141 tcagcatgat gactacaccg cgaatcccgc catcttgaac gatcagattg cttgcatttc
    28201 ctaataccat ccaaggtata tcatggattc ggcaataatc gatgatttgc tttacttctt
    28261 cttgtttttt cgggaatgca aggacatcag ctggtccacc tgttttcgta aacgtatagt
    28321 tcatcaatgg ttcatcaaac aatatagtaa tcgtggctaa gctttcaact atttcttctt
    28381 tattcactga ggattttcct ttcttttctc aaacgtcctc attattctat cacgcttaat
    28441 acaatcttgc cagtcttgtt caaagttgat gacggtagtg atctttaagc acttcctcac
    28501 ataattcatg acttcttaca acttcttttt gtttaagcaa ttctgtttcc gatttaccta
    28561 aacggatctg agaaaagaag gcctctacca ttggctcaaa cccgcgtttg actagcgtgt
    28621 ttgtccagtc gccaaattct ttcacttttt ttccatcagc ctcttgaatg acaagctggg
    28681 tcagatcgtt tacttcataa gtaccatttt tacttgttac ttgatatttt tctatattag
    28741 cgccacttgc taaatccatc gttaaaatag ccgtttgatt ttgtgtttct aattgtaaaa
    28801 tggcatattc caaaaattcc tttgtttctc ttatctggta ttttgttctc aaaatgggtt
    28861 catctaataa atacacagct gtatcaataa gatgtaaaaa caaatcataa aggacgaact
    28921 ctgtcgtttc ttttgccgct attcgatttt tttgcaggtg gatcaaccgc ttatctggga
    28981 tacgtttcaa ttcttctacc ataggagcaa agcgccggtt gaaaccgatc atcaaaatta
    29041 tttggttctt atcagctaat tcttgcaatt cacgaacttc atttagatgg acactcagtg
    29101 gtttgtcaat ataaacatgg ataccatttt ctagacattt tttggctaat tcataatgta
    29161 cttttgttgc tgcgtggatc atacatgctt cgattttttc ttctaaaagc tcatcgatcg
    29221 tttcatatag atgttggaac ccgtactctt tttgaattct ttcttttgtt tctttatttc
    29281 ttgttgcaaa ataaaaatca agtgttcctt gttttttcgc ataagttggt aaatatgctt
    29341 tttctgcgat attccctaca ccgattacac caatcttcat ctactttcct cctggatcat
    29401 ttagttactt tcatcttaca ccatttttta ctaaaatgag gtacaatagt atcgcaaagg
    29461 taaggaaatt aattcgaagg agtgtgcttt tattgaaatt tatctcttgg aacgtaaacg
    29521 ggcttcgtgc catcgtaaat aaaaattttt tagaagtttt tcatgagtta gacgccgatt
    29581 ttttctgtct gcaggaaacg aaactccaag caggacaaat tgatttagat cttccaggtt
    29641 attatcaata ttggaattac gcggaacgaa aaggctattc cggcacagcg atttttgcta
    29701 aaaaaccagc attgaatgcc acttacggaa tgggtatcga tatccatgat acagaaggcc
    29761 gtctgattac tttggaatat tccgatttct ttcttgtaac ctgttacacg ccaaattctc
    29821 aaagcgaatt aaaacgttta gattaccgat tagaatggga agaagccttt tataattatt
    29881 tggaaaatct aaaaaaacaa aaaccagtca tcgtgtgtgg ggatctaaat gtcgctcatc
    29941 aaaagattga tttgaaaaac tggaaaacga atcaaaaaaa tgctggattc actccagaag
    30001 aaagagcggc tctttctcga ctgttggata acgggtttat cgatacattc cgttattttt
    30061 atccaaccca ggaaggtgtc tactcttggt ggaactaccg attcaattca aggaaaaaca
    30121 atgctggttg gcggatcgat tactttttga cgagcaaaga tttagagcca ggactagctg
    30181 atgcaaagat ccatacggac attatgggca gtgatcattg tccagtagaa ttagatttga
    30241 aataaagagg ttgtgaaaaa gtcgtttagt ttcgagtatt aagaaaaatt ttgtgaaaat
    30301 ggctttccac attttgcata aatttggcat aataccgaga aactggcttt tgaacactgt
    30361 ttatcgagag attgtgaaaa agctgtcctg catcaagtaa taagaacagc caaacaaaaa
    30421 tagttcctca tatttttcgt ttggttgggc ttattaccgc agatacaagc ttttgaacac
    30481 cgtttattaa gaggttgtga aaaagctgca ctgcatcaag taataagaac agccaaacaa
    30541 aaatagttcc tcatattttt cgtttggttg ggcttattac cgcagatgca agcttttgaa
    30601 caccgtttat taagaggttg tgaaaaagct gcactgcatc aagtaataag aacagccaaa
    30661 caaaaatagt tcctcatatt tttcgtttgg ttggacttat taccgcagat gcaagctttt
    30721 gaacaccgtt tattgagaag ttgtgaaatc agcgtcctga cctgagtatt agagaaacaa
    30781 atggcaaaat agttcctcat attttgctat tttttgagct aataccgagg gtcagctgat
    30841 tgaacaccgt ttagtggaga agttgtgaaa aagtcgccta ctttcgagtc gtaagaaatt
    30901 tttttgaaaa tggttcctca caactttgca taaatttgac ttaataccga gaagtcatga
    30961 tccccatcta actccaaatc acaaggaatg agttcaaaaa ctagcttttc tcaacaaata
    31021 ttacataaaa aattctatgt tttttgttaa atttatttgc ttttttcaaa agtatgttac
    31081 agtaatttcc caaggaggtt ttacagaaat gaattttgtt gccatggatt ttgaaactgc
    31141 caatcatcag ccttatagtg cttgttctct tgcgctagtc atggtaaaaa atagccagat
    31201 cgttgatgaa ttctatacat tgatacagcc tgagactcct tttttctggc gaaatgtcca
    31261 aattcacgga atccatcaag aagacgtacg caatgcgccg aaattcccag aagtctggca
    31321 aactatccaa ccatattttc aaaaaaatcg tttagtcgtt gcacataatg ctgcttttga
    31381 tacaaaggtc ttagcgggat gcttagatta ttatcatctt gaacagccta attatctttc
    31441 tttatgtaca gtgaaaagta gccgtcgcct gtttcctgaa tttccaaatc atcgattgaa
    31501 cactgtgtgc gaaaatttga acatccaatt agatcatcac catgatgcat tggaagatag
    31561 tcgtgcatgt gcggaaatcc tgctttatca agaaaaaaaa ttcgggacag atccattgaa
    31621 aaaacttgtg actataaagt aaaagaatac ttatgaaaca cacaaagagg tcgtgagcct
    31681 gccgtttcac tccgagtcac aaggagcaat ttcaaaaaat agcttctcat gtttattaaa
    31741 tattgtgact ggtgaccgag gagctggcag gagaacaccg tttattggag aggttgtgaa
    31801 aaagctgttc tgcatcaagt aataagaaca gccaaacaaa aatagcttct catatttttc
    31861 atttggttgg acttattacc gcagatgcaa gcttttggac accgtttacc aagaggttgt
    31921 gaaatcagcg ttttgacctg agtattagag aaacaaacgg caaaatagtt cctcatattt
    31981 tgccgttttt tgagctaata ccgaaggtca gctgattgaa caccgtttat tcaaaaaaaa
    32041 tggactgcga cacgactttt gtcacagtcc cttcgtgtgt ttttctgcct tctcaaaagg
    32101 atgacagttt ctcactcttt tcctatttct tctattcaat caaataactc attttatata
    32161 catctaaaaa ttcacctaga tctgttcttg cccctctagg catgaccgct tcgatttgga
    32221 agcccatttt tcggtaaagg tgtacggctc tttcattacg aacttgaacg tcaagctcca
    32281 atcgaaaaag tacacccatt tccttcgccc aactgatgat ttcttctagc ataagagtgc
    32341 ctagtcccat tccccaatat tcctgcagaa tactgatacc gacttctccc acgtgagaca
    32401 aacgaaattg actgtctgcc ttgacagatg ctgttccaat aatcgtccct tcatagattg
    32461 caagcagcaa taaattatta ttgctttcgt atatataatc caactcttct tctaaggttt
    32521 ctggtggtaa caggagttct gcttcatcca aaactaaaaa ttctgtctcc ttattaactt
    32581 gtcccatcat caaaagaatg tcttttgcat ctcctggcac tgcttcccgc aagatgatcc
    32641 caacttcttc actcatgatt ttcagcccat ttttctagaa ttcgctgttt gaaggtttca
    32701 ctttgctccc cgtatgagat tagtttcaca taccgctttt ctaaatctgt atcacttttt
    32761 tctaaaatca gttccaaata atcctccggc agtgcttctt ctaatagatt tccccattca
    32821 acaacggaaa gcccatcacc ttcaaaatat tcatctaacc ctaaatctgc tccgctagct
    32881 gctacccgat aaatatccat atgatacaac ggaatccgac cttgactata ttctctaata
    32941 atcgtgtatg tcggactttt gatcatttgc gttatcccta atccttgggc aattcctttc
    33001 gtcaacgttg ttttacccgc acctagatca ccagtcaata tcagattgtc acctggcatt
    33061 gcggaatgac caattgcctc tgcgaattgt tctgtcattg ttaatcctga tagttcgatc
    33121 atttttcatc ttctcccgct cttctttctt tttgccagta tatcgcgtaa tgaagttttt
    33181 ttaaagaaaa aatgaactag tcaagaacag cagctgctgt ttttggctag ttcgcgtgta
    33241 tttgttaatc aatcaatgtt tgtgctgctg tgataatcga taatttatat acatcttctt
    33301 catttgctcc acgagacaaa tcagaaaccg gtttgttcaa gccttgtaga attggtccga
    33361 ttgcttcaaa gttaccgaaa cgctgagcaa ttttatagcc gatatttcct gactgcaatt
    33421 ctggaaatac aaagactgtt gcttgaccag ctacttctga atctggcgct ttcagttgtg
    33481 caactgaagg aacataagaa gcatcaaatt gcaattcgcc gtcgatcatg tattgtggcg
    33541 ctagtttttt agcgatttta gttgcttcaa ctactttgtc tacttctggc gatttcgctg
    33601 agccttttgt cgagaagctc atcattgcta cttttggttc gatatcgaac aattctgctg
    33661 tttttgcaga atctacggcg atttcagcca attcttgcgc agttggattc acgttgatcg
    33721 cacaatccga aaagacatat ttttcttgat ctcttccacg taccatgatc atcgctccgc
    33781 ttgtccgact gatgccaggc tttgttttga tgatctgaag tgctgggcga acagtgtcgc
    33841 cagtcgaatg gatcgctcca gagaccattc cgtctgcaat ccccatatac gtaagcatcg
    33901 taccaaagta attcacatct tttaaaatct tttctgcttg ttctttggat gcctttccat
    33961 ttctacgttc aacaaaagta tccaccattt catcccactt atcgtaatta tcagggctga
    34021 tgatcgtaaa gctagatgtt ttgattcctc tggcatgcgc tgcatttctt atttcttctt
    34081 cgttccctac taaaatgggt tccacaagtt cttctgcttt caaccgggca gctgcgccta
    34141 aaatacgagc atcagtagct tccgggaaaa cgattttgat cccacggcga acaattttaa
    34201 atcttaaact atcaaacaat tccacaagat gacctccaga cgtttttata tgtattatca
    34261 tacactattc cagaaaaaaa gaacagttta ttttatctgt atcagtcaaa aatgctggaa
    34321 aaattaagtt tttcacatac aaagcgaact gcactataac aggaaagcgc tgttttatta
    34381 cactgtttca ggcagttgcc aatcaatcgg ttcttctcct aacgcaagga gcgcatcatt
    34441 cgttttcgag aagggtttgg aaccaaaaaa gccacgactt gcagataacg gactgggatg
    34501 tggagatgtt atgatgatat ggcgactctt atcgatcatt ttgatttttt cttgtgccgg
    34561 ttttccccac aagataaaca cgattggttt ttcccgttca tttaattttt caatgatcgt
    34621 gtctgttaat ctttcccaac ctttcccgcg atgagaatac gcttgccctt cccgaacggt
    34681 aagaacggtg ttgagtaaaa ggactccctg tttagcccat gacactagat ttccatgttg
    34741 aacaggtgcg attccaagat catcataaag ctctttatag atatttctta acgatggcgg
    34801 gatctttacg ccaggttgca cagaaaaaga taagccatga gcttgatttt ctccatgata
    34861 aggatcttgt cctaaaatca ccactttaac ttcttcataa ggagtcaatt ccaatgcttc
    34921 atagatatga tacatatctg gatggatctt ttgggacgca tattcttttt ttaaaaattc
    34981 tcttagatgc agataataat cttttgaaaa ttctgaggac aaaacttctt gccaactgtt
    35041 atgaatgatt tctttcaaaa actccacctc ctaataaaaa gaataactca cattttcttc
    35101 aatcacaagg gacaatcttc accctcagca cttgattaat atggtaaact aggaacaaga
    35161 agcagaaagg aacgataatc attatgatca aattaattgc atcagatatg gacggcacat
    35221 tattagatgc ccatatgagt gtttctcctg aaaatgccga ggcgatacgt tttgcaaatg
    35281 atgccggtgt tgagttcatg gtcgctactg gtcgaaacta tcaagaagct cgcgcagctt
    35341 tagatgaggt aggaatcgat tgtgcgatga tcactttgaa cggggcacag gtatttgata
    35401 aagaaggaaa ttcattgttt accgtgtcga ttccaaataa tcaagcaatc tctgttttag
    35461 atattctaga tgctaacggt atttactatg aagtagcaac caacgatggt ctttattctg
    35521 aaaatcaagc gaaacgaatt gaaagttttg cttccatggt ggctacccat ttacctcacc
    35581 ttacgtataa aatggcgatt gccatggcct cagctaagct ggagttattg cacatcacat
    35641 acgtggacag tattcgtgct attttggaag aaaagaatct ggaagtttta aaaattattt
    35701 gttttcatac agaagggcca gctgtattag gtccagttgg aaagcagatt tctcttttag
    35761 gcgatctagc cgttacttcc tctggtcaaa acaatatcga agtgaaccat aaaaacgctc
    35821 aaaaaggaat tgctgttgcc catgtcgctc acgagcgtgg gatttcttta gaagaagtca
    35881 tgacgattgg cgataacttc aatgatgtca gcatgctgca agtagcagga gtcagtttcg
    35941 caatgggaaa tgcagaaatc gaagtaaaag attatgccaa atatacaacg gacacaaacc
    36001 ttgaatctgg cgtaggaaaa gccatattgc gcgcgattgc agaagacttg tagttttatc
    36061 tagaaaagag ggatatagat gtcagaattt ttcattcagg aaaagcagct tggacgtgca
    36121 actcggacaa ttgtcaaaaa cgaaaaagga cattccctgt ttttgatggt tggtcgctgg
    36181 ggaacaaagg gcgatgcatt gtctctttat gccatgaatg gcgatctcgt tgctcacatc
    36241 aagcagataa gcctgacatt cggtacgcgt tttgagctgt ataaagagtt cgaaaaggtg
    36301 ggaaccatgc agaaaatatt caattggccc ggtgactttt attatattcg tagacttcac
    36361 tggacagtcc aaggtgatat ctataatcat caatatcaaa tccatcattt caatcagcaa
    36421 atcatgaaaa tggataaagc aacacttttt tcaggagatt actatgtatt ggatgtctca
    36481 aatgatgaag atgctccagt ttgtatttgt attgctgcgg ttttagatta ctggctctac
    36541 cgtaaagaca aaagtcgcga taagcctgtt tcttaccgac ttaacttaag ttaaaaagat
    36601 gtacctgaaa tgcggaaata cgcatttcag gtacatcttt ttagatattc ttttgcatat
    36661 gatcataaag atgaccactg atcatccgct gaccgacaac tttgaagtca tggcgttcgt
    36721 ataatttttt agcctttgga tttttttcat ccacacttag accgatgacg gagcgattag
    36781 ctttcttcgc caatttaggt aatgcttcta ataatcgcga accgatgcct tgtcctctaa
    36841 aatcttctga tacactgata gagtccaaat accattcgtt gggaaacgtt tctttatcaa
    36901 tgaacaaacg tgtagacgca tctagctgat atttctctaa gataggtacc agtggctgat
    36961 cgatgattga ttcttcttca tctgtataac cgtaagcaac tcctgcaact ttaccgtcta
    37021 tttcttctac gatacctcgt gaataactgt aacgataatt tggatcagtg attgcttctt
    37081 ctaaaatctc taaagtcttt tgttcccctg tctgcttcaa aaatggcagt tccatatctt
    37141 ttagaatcac taagatcaat ggggcgattt ctggagcatc ttcttttctc gcaaaacgaa
    37201 tcatagtttc ctctcctttt ctccattcta acatgcagaa aaagaaatcg caaaatcgtt
    37261 attttaaaaa atcttcaatc ttttcagtaa tgaaataacc atgagtaacg attcttgccg
    37321 cttcagttcg gcaataaggg cagacgactg gcatttcttt atcattatgt tcgactacat
    37381 tattgacttc ttcgtatgca aatttttttc cgcaattagg gcatttcatt ttttccatgt
    37441 tatctcaact tcctttcatc tttactctct tagcttactc ttatattgta aaaaagagaa
    37501 gcaatatcct tcttctatgc agatatccat acctgacggt cctgttctcc atctattaat
    37561 cggactttat aataaggacg ttcatctttg actagaagtg tccactcttt cacttttgct
    37621 tttttcccgt agtagctgac tgcttccttc atcgctgctt gcggttgttt gaactcttcc
    37681 gttttttcta tatcaaaaag atgttctgcg gccttttccg tgtcatttcg tacggttgtt
    37741 ttcccagtgg atggattgac tgctatgact tgtccttcac aaaaaaactg gtattcatat
    37801 gttgcattcg taggattctc caaatctttt tcaactgcaa aatgaatagc agatatgtta
    37861 tgttcttttg tttcgttttt tacgattgct gctgcttttt tcaatgagat ccgatattcc
    37921 tggtcactaa tatcatccat gtgaaaagcg gcattgagca gtcccgtcat accggtaatc
    37981 cctattacga tgagggcagc aacaccaaac atcatttttt tcattcttca ttctcctgtt
    38041 tctttttttt aaagaatatc aagaaatgaa cgaatgaaaa atcgaatgaa acgaaaaaaa
    38101 agtgacaatt ttgttatctt tattcgtgca gttctaatgg caatccgtct ggatcaaaga
    38161 aaaacgtcat tttttttcct gtaaaggtat cttctctaat cggttctgat tcgatcccta
    38221 aggactgcaa ttctgcaatt gtttcttcaa tatcgtctac tttgaaagca agatgacgca
    38281 agccacaagc ttctggatag tttggtcgtt gtggcgcatt agcaattcca aaaatctcca
    38341 attcagcact tcctagtttc agatctaatt tatagtcatc tcgttctttg cggtaatttt
    38401 ctctgattac ttcaaatcct aatttattaa cataaaaatc acgtgatttc tgataatctg
    38461 aaacaatgat ggctatatga tggatcgttg taaggttcat atgagcactc ctattcttcg
    38521 tttaagctcg gatctatatt aatctccagt tccattgtaa atgtgtcaga ttgaaaaaaa
    38581 tagctgtttt tcctttttat aagaaaaaag gcaaaaaaaa cagaaagtag gtcctccaag
    38641 acactcttcc tgttcccaat ataataaatg aaagtaagaa attatatttt caagaaaaca
    38701 aacagaaaat tatcataaca caaattattt ataattgaaa gactagtttt ttttcaagaa
    38761 aagcatacaa tagattgtgt tagtaaatac agaacacact acatttagtg tcttaaggag
    38821 tcctcataca tgaagtcata ttttcagttt ttagctcatc agctaaaagg ctggccgcaa
    38881 caaaattatt atcttttctt tttcagtctt ggctgccagg tcatgactct tgtcaatagt
    38941 cccatcactt ggctgtccgt tattacattt atcggtacca cgcttggtgt attgtgtatt
    39001 ttatccatca atgcggcaaa atcagtcaac ggtattttag gtattttgtc tgcgatttgt
    39061 tttattatcg ttgggttttc agcaaagaac tatttaagta tcggtgaaca gattgcttat
    39121 atgatcacat tggatatccc cgtattgtta agtaaagaat ggaatcataa tatggcttca
    39181 aaaatccgaa aattcactgg aaaaagctgg tttatcgcaa tcatcagtac tttccttgtc
    39241 tatcttgttt caggattcct gattggtcgt tatacagatg atcctcggcc gtgggtagat
    39301 gccattgctt tttctattag cttgactgct ggtatcatca gtttcttgcg ttacaacaac
    39361 caatattact ggtggttagc ttcagggctt gctcaaatgg tcctttggta tatttcattt
    39421 acgcatggat ctgcaagttt ggcgatgttc gtgaacagct ctgtttactt gctgaatgat
    39481 gtccttgcat ttaccgtttc accttggtat aaccaaaaag aacgtgaacg tatgcaaaaa
    39541 atggaagaag aatactacaa cgcacaacaa cacgcataaa aaaactgagc gtgggacaaa
    39601 agtaaagaac acttttgtct cacgctctaa acacgtataa acggcggaat cagaagcaac
    39661 tccttcggaa ataagctgaa attcacaaaa atttgaaaag caattttcgt gaatttcttc
    39721 ttatttcttg gagttaaacg cttctgtccc agcctctatt ttttatagta attccttcag
    39781 atactttctt gcctcatcat tgaaacgcag tgattcttct aacttctgat aatattccaa
    39841 cgcttgtttc tgagcttcag taaaacgata atgttttcct ttccatttgt cgattgtctc
    39901 ttgcacattt ccagattgga tatctttcac tactagttgc catttttctt ttgagactgt
    39961 acggtctttc ttatcagcag attgtatagc ttctcgttct ttacgcaata cttttaaaaa
    40021 accatttctg actatccgag gaatgctgtc gaagaaccct tctgtaaaac catagcgaca
    40081 atattgcttc caaataaata gatagatttt tggttggatg aatcgttggt ttttcgcaaa
    40141 atattccatc tgagcagtga cttctggtgt tcgttgacgt tttgtctttc tcaacacgga
    40201 aataaatgtt tcaaaataag cattaggatg tactggcact tcttcatata cgatggattg
    40261 ccaatcttta cgtaaaaatg cgcgatagtt gatcaagaat tcttctacat cctttactaa
    40321 ttcttctgca aagtcgtcaa atagttcttt ttttatctgt cgcttttttg actgcccatt
    40381 gatgtgttca cttattttct tgatttcatt ttccaatgct tgataatccg ctatgtaaat
    40441 cggcatttct acttggcgaa attctgcgag ccgctgagct aattttatat ccggtaaata
    40501 tggctgtttc atccgacgat tttggtacaa gacaaaacag tatttttgcc atagtccttc
    40561 aggaaaatcg atattcttct gcttaagcca taataattcg tctaaggcca gatcaacttt
    40621 tgtcattccc tgatggatcg aagctgccac tgtcggagag acatgcaggt gatcggcaaa
    40681 gtggttgatg cctagtttta ggatctttcc ggtttttggc gatttaacga tgtattgcac
    40741 ttttaattct ctaccacact cacaatacaa tttttgctgg cttcgttttg ttcgaaaaaa
    40801 cggattgatc ttgaagtcag aaaacaccca atctgttgca gctaaataat gttcttccat
    40861 aaaacgagag ctgattagat atcggcggtg cttatcgact agttcccgct gttttttcgt
    40921 taattttgca tagatctccc aacgttgact gcttgtgtat tgtttagaaa ttttgatggg
    40981 atagcttttg ctgatttctt ttggaaacca ttcttcaaag acggattgcc attcttccac
    41041 ggctgtttct ttcctttcta attacaaaaa gctggctact aacataacgt tagcaaccag
    41101 ccagtgtttc ttgctaaatt agtgagttgt tccggcattt ccggaaatat cttctttatt
    41161 ttctactgct gcttcaactg cataagtcaa agaagaagca atgtcttcta aagacataga
    41221 aggcgtaccc actggacgat cgatcacttg atcgggtgag aatggcacgt ggatgaaccc
    41281 acctcgaatt gccgggaatt gtgtttcgat caaatgaagc aaacgataca tgatatcatt
    41341 gcagacaaat gttcctgctg tataagagat gtacgctggt agaccatgat cttgtacatt
    41401 ttttgtcatt gctttgattg gcaatgaggt aaagtaagcg gctggaccgt tttcttctaa
    41461 agccgtatcg aatggctgat ttccttcatt atcagggatt cttgcttctg ctagattgat
    41521 tgcgacacgt tcgaacgaga ctgctgaccg cccacctgct tgtccgacac agatcacgac
    41581 atctggttgg tgctgttgga tggcttcttt gactacttga ctgctttttt cgaatacggt
    41641 cggtacttcc actttgatga tttcagcacc gctgatttca tctggcattt tttttactgc
    41701 ttcataggct ggattcactt tgtccccacc aaatggatca aaacctgtta ctagtacttt
    41761 catctgttgt tcctccgtcc gattgattta acatatggtt atcttttatc ataccatttt
    41821 ccttgaacag cgtaaataaa ggaaacaata gtttaaataa cataacagat caaacttaat
    41881 atattcttat aaaaaaatag tgttccacta caatgttaga agaggttgcg acaattattc
    41941 tgtcacaacc cctctcttct attatttaaa tagaatcata taagcaattt ggaaaactag
    42001 cataaataac gcaataaaca actggttttt gatcactccg tatttgtctt tcatctccag
    42061 catcgctaca ggcacgatat tgaagttagc agccatcggt gtcagcaggg tcccacaaaa
    42121 accgcaagtc aatgcaacca ttcctattaa cgctggattc gctccatgac taaatacgaa
    42181 tggtgcaccg atcccaaccg tcatcacggt gatggccgca aatgcattcc ccatgattac
    42241 cgtaaatatc accatgccaa gagcgtaaat gatgatgccg atgttcacat ttccttctgg
    42301 aacgatcgta cctactgcat tggaaatcac tgtcccaact ccagcgcttg taaataccgc
    42361 acctagagaa gctaaaagca ttggcaacat gcttaacggc cctactgtgc ccagcatatc
    42421 tgctgcatca tctaaaaatg tacttggtcg attatctcga gaaaagaaca tcaagattag
    42481 catggctgca aaaacaccga cgcccacgcc gactaaagca cctaaattgg taaatagagc
    42541 aaacaagatc gcaaagacac cgatactcaa agctggaata aaaattttca ttcctagttt
    42601 gtctgacatt ttttccatat attcttttga tggaagccgg ctttctcctt tgctgacttt
    42661 cttagcaatt gctggtatgg tcatcgcaaa gatcaagcca ccgttcacga ttgccggaag
    42721 ccatcttcct cctgcaataa ctacccctaa gaccgtccag aaaaaggctg acccataacg
    42781 atgaggattg gttttatctg ttaaatttct tactccagtg taaatcaata cgagtcccat
    42841 cacgatataa aggatctcca gtaatttatc gcctaataaa atatcaggat ttgtaaaaaa
    42901 gtccatctta ttgccccttt cctggataat atttcttcgc taatttcttt tctttaagat
    42961 agaagtagac acttgccaca actaaagccg tgatagccac tggaacttcc actaaagcaa
    43021 gctgtcccaa tgttacgtca tatcctagat ctttcagtgt tgattggact agcaacgccc
    43081 ctgctcctcc gatgaatagt acttggccaa agaaccagca gacattttcc atagctgaag
    43141 ctataccttt tagttcttct tcatgcttag gatggacttc taaccctttt gattcgatgg
    43201 tccccaatgc catcggtaaa atgattggac gaacaaatcc agcaactcca ccaaaactca
    43261 cgttaaaagc cgcaaagatc attcgaaaga tcccataaat atctacaatc ataccagcag
    43321 atactttctt gaatcgcttg atcaaggaag ccgctgattc tttcagacca tttctttcaa
    43381 gcgttcctgt tgccaacata atgatgataa aaatcgccat cccgcgattg tttacaaaac
    43441 ttgtacctaa aagttctaac attccttgaa tgccaagacc gccagtcaaa gctgtaacaa
    43501 ctaaagcaac cattacgatc aatatcgagt ctaatttaaa tgcaaacccg agaataacaa
    43561 tcaagacgcc taaaaggcta agataatttt ccatatattt tcctccataa aatgacaata
    43621 tttttttaat tataaagcaa aacacataaa aaatatagtt gttttctcac gaaatagaca
    43681 ctatttttaa atatttttcg ttattttttt atcacaaaaa ttatcttact tattgtaagg
    43741 taaagctttt tttaatttat tgcccgataa tatatagaaa taaggtctag ttactgtttt
    43801 ttttaaatga ttaataaata acaacttctt tctatgaaat tgcaaagcat tcatcttctt
    43861 ttccgctaac gatatatccg tcagtgcttg gtacacttta ctaaaaagta tgagattgcc
    43921 gatatctgga taggtgtgct atactacaga aaaagatata caatctgcca ccgggtagga
    43981 taaagaggat gatcattcca ttaggtcttt gaagggatga ttttcctctt tttgtgtatt
    44041 tttttatagt taaggaggaa tgtaagtatg gcatggatgg aattaatcat agcaggaatt
    44101 ttggaagtat tctggtcaac gatgatgaaa tggagtgatg gtttttctaa aatcaattat
    44161 tctttttaca cagtgattgg catgattgct agtttttact ttttgtcaaa ggcaatcaaa
    44221 agtttgccga tgagtctctc ttatcctatc tggacaggga ttggcgcagt tggttcggtc
    44281 ataattggtg tggtcttgtt tcatgataag ctgaatcttt ccacttggtt ctttgtgggg
    44341 atgctactca tcagtatcat tggcatcaag attactagcg gccactagac acttggaata
    44401 agtactgatc agaaaaaata agccttttta tttaatattc aattttttta tgaaaaaata
    44461 agaaataagt ccaatgacct agcgacttct ccactcatca ggtgtatgct tgtactatct
    44521 atagaaaagg agtgaatatt tttgaaaagt ctttcggtcc aagaattatc caagaacttt
    44581 ggtcacaaac aagccctcag cgatgtcagt tttagcttat cccctggcaa aatcgttgga
    44641 ttagtaggtc ctaatggcgc tggaaaaaca acgatcatga aagctatttt aggactgatc
    44701 cattactcag atggaacaat ctgtattggt gaaaatgaga tttctccctc tagtcataaa
    44761 gggttggaac aagtaggagc attgatcgaa tacccgggta tctatccatt cttaacaggc
    44821 tatgatcatc tgaagttatt ctctgaaacc aacgatgtat ctgctatcga cactatcgtg
    44881 aatcagctca agatggaaaa gtacattcac aaaaaagcga aatcctactc gttgggaatg
    44941 aaacaaaaat tgggaattgc tcttgcctta ctgaaccatc cgtctatcgt tattttagat
    45001 gagccgatga acggtttgga tccacaagca acaagagatg tgcgaaaaat cattaccgac
    45061 ttggctactc agggcacctc tttcttgatc tccagtcact tactcagcga gcttgaaaaa
    45121 atggtcgatg agctgatcct gatcaatcaa ggaaagatca tcaaacaatg tacaatggct
    45181 gaattgaacc aaacagcgca agatttcatc gttatctcta caacaaatga tctagctgct
    45241 cgaacgatct tgacagaagc tggatatact ttggaagatc ctgataaaat caagctttta
    45301 aaagaaaacg aagaattatt aggaaccgtg atccagctat tgtctgcaca aaatatccat
    45361 gtgacagacg tccagcatct gacgaatgat ttggaatctt ctctattgga aattctggat
    45421 tcagtaaagg gggaagaaga aaaatgacat tgcttgtaaa acaagaatta tttaaattga
    45481 tcaagaaaaa gtcaactgct gttttatctg ttctattagt tgttttgctc ataggaacag
    45541 cacttctagc caaaaaatac acgacgatca ttgatccagt tgaaatgact gctcagcttt
    45601 ttagtgccac atcttggatt gtctttatca tgattgcagc tgcaagcacg atcatttcga
    45661 tggaagcaca atatggtaca ttgaaaaatc tcctttatcg aaagtattct cgaggagaaa
    45721 tattagtaag caaatggatc acattagtta tttattctgt ttatttatac cttttagcta
    45781 ttattgtcac tgtgttgatg aaactgattc ttttcccttc gatttccttc acggagaagg
    45841 tttctactgg tcaaacactg atccaaagct tgttcactta tactttgggt agttatatcg
    45901 gtttatggct gattttaagt ttagtgttga tgattgcttg ctttatcaat agttccggcg
    45961 catccatctc agcaggaatc gtcttttatt ttgcttcgtc aatcatatct ggtatcctta
    46021 ttgcactcat ccaaaaatgg gagtggatca aatggaatcc gatcagtatg ttgaatctgc
    46081 aaaaccaaat tggaaacgaa gagatcatga agcaattgac tcatctttct actaatcaaa
    46141 tgctattcgg taatttagct tacatcgttt tgttcttagc attaggatat ctcgtattta
    46201 agcgaaaaaa tgtatgatga ctcatcaaaa aagaggctgt gaaagagacg ttcagcctta
    46261 ttgccgattg gctgtctctt aaacaccgtt tattctgaaa aaggagctgt gacaacattt
    46321 gtgtcacagc tcctttccta gtatgttttt gaataaacga caggctcaca acctctcttt
    46381 atacttcttt tatacttcta tacgtgaagc atcccagtgg caaataaaac cattgcaatc
    46441 actccgacca tcgtcacacc tataagtaaa atcttatcta agtggatatg ttgtacaggc
    46501 tcgttttgct ccttacgtgc tttgaggtaa aaataaatgc ctggcaagaa aagaacaaga
    46561 acaagcatta cttcattaaa ggctacgact agcatagccc atacttgaaa ggcacttgct
    46621 aaaagcccta ctaaaaattg gacccattct ttccgctgat aagaaagttt catttggtac
    46681 aatcctaaga aaagccacgt gaagaaaata gctgctgtac caagtgtgta agcaaagtta
    46741 taagcactgt cagtgaataa taatgaaagt aaaaagacat tcgtacataa cgtcgtcaat
    46801 acgatagcat aagttggtgc cccatttttg ttgagttttc cccatgcttt aggcaaaagt
    46861 ccgtcttcag ccatgtttcc cattgtttca atcggtaaga tcgtccatga gagccaaaca
    46921 ccaactaaag aaatgatcaa accgccgttg atcacgactg ctccccattt accaaccatc
    46981 atttcaaaca catagcccat agcaggctgc gccttgatcg tttgcagctg attcatcgtc
    47041 attgtcccat atggaagcat ggaaaccaag acgtaaataa taatcaagcc gaatatccct
    47101 agaatcgttg ctttcccaac atctgattgt ttttttgctc gtgaacctac gacagaggct
    47161 ccttcaatac caacaaatac ccaaagcatc accatgatgc aactcaatac ttgactggaa
    47221 aacgggatcg gctgtccatt cgcattcaat ataatgtcgc tagaaaagtt atcaatgaat
    47281 tgttggatcg aaaacgtgct gatagctact acaataaata aaaataatgg cactaattta
    47341 cagaatgtga cgatagcatt gacgaaacta gcattttcga ctccccgatt cactagccat
    47401 gcatatcccc ataaaaagac tgtactcatc acgatcgatg ccagattttg cccaccttca
    47461 aatactggga agaaatatcc taatgcgctc atcaacaatg ttgcaaaagc tacgttgcct
    47521 aaccagcatg tgagccagta accccaaccg ctgataaaac cagcgaaacg gccaaaacct
    47581 tcttctgcgt aactaaagat cccgcctttt aaatctggac gttttttatt gatattattg
    47641 atcgacaagg agagcatcaa cattgctgtt cctacgagta cccaactgaa aatagctggg
    47701 ccgccagccg atccggagat gtccgtcata ataccaaata cgccacctcc aatcgctgaa
    47761 ccaattacta aggcagcaag tggaattaaa ccaattcctt ttttctgttg ctctgtatcc
    47821 atgtaagcat cctccaaaat aaaaccgctt ttatcaaaaa gtataacaaa ccttttttat
    47881 ttttacttaa ttaataaaaa ctttaataga aatataattc taccggcttt tttctcatta
    47941 tacaagcaaa gcgcctagtc cctttccaaa ttacaccacc aaaaaactta aaaaagatgc
    48001 tatactaaaa ggaaagaatt ggaggatgat ccatgaacat tttatatata tcgatttcag
    48061 gaaatacgcg tgcctttgcc aaacatctag cagaatatgc tgaaaaaatg catgcagaag
    48121 atcctgtcaa tcctgaagtt actttaaaag aaatccatga aaatagtgat tttacgaaag
    48181 aaacagaacc attttttact ttcgttccta cttatttaga cggcggaaat ggtttagata
    48241 acggtgatac agaaatcttg accgaaacga tgcgtgaata tctggaacat gaagacaatc
    48301 ataaactttg tcttggtgta gtcggtagcg gaaataaaaa cttcaataat cagtattgtc
    48361 taacagccaa acaatatgca caaacatttg gctttccttt cttggcagat tatgaattac
    48421 ggggaacacc atctgatgtg acacgaattt atcagatttt agcagaaaac aataaataat
    48481 aaaaaagaag aagctgaaaa atagatttca gtttcttctt tcgtttctat tgtttctatc
    48541 atattaagct ttgacatcca tcacaggtgc ttttgctttt gctttacctg ttttttccaa
    48601 gacaaagttt tttacacgat ccatattggt taccaagacg atcatggatg tttctttgcc
    48661 tgctgctttg atcgcttcta agtcagcttc tgctacaagc gtatcaggtg tcacttgctg
    48721 cccttctgtt actttcacat caaatggcgt accgcctaag tcaactgtgt tgattcccat
    48781 atgaagcaag atttctaaac catttggcgt tgtgatccca atagcgtgtt tcgttgggaa
    48841 gacagagctg actaccccag taactggaga atagatatgt ccgttttctg gttctacaaa
    48901 gtagccttca cccatcatct tttgtgcaaa aactggatca cttgctttat ctaaagaaac
    48961 aactgttccg tcagcaactg atgagaggat agtcacactt tcagctgaag tttgaggtac
    49021 tactttttca ggcacttcat ttactgtggc tgtttcagtc gctaattctt ctttttctaa
    49081 acggtcttta ggaataccga agaagaatgt agcaacgaag ccaccagcat aagctgccaa
    49141 taatccaagg atgtagccta accatttacc gtcagagatc aatgggatca aagcggctcc
    49201 tgaaggtcca atagcgatag ccccgacatt tccgatcatt ccgatcactg ccccgccgat
    49261 acctccaccg atacaagcag taataaatgg gcgtcctaga ggtaatgtta ctccgtaaat
    49321 caatggttcg ccgattccta ggatcccgac tggtaaagct cctttgatca tttctgttaa
    49381 ttctttatct tttttgcagc gaatccataa tgccaatgct gcacccactt gtcctgcacc
    49441 agccatcgca agaattggaa gcaatagcgt acgaccagtt tcatcaatca tttgcacatg
    49501 gattggagtc aaaatttgat gcagtccgaa cataaccatc ggcaagaata atgtgcctag
    49561 aacgaagccg gcaaatgctc caccaacgcc aagtacccag ttgatcccac cgaccatgcc
    49621 atcagaaatg aaacctgcta aaggcatgat gaagaaaatt tcaatcagac cgatggcaat
    49681 caatgaaaga gttggtgtaa cgatgatatc taccgcatca ggtactactt tatgcagttt
    49741 tttctcaacg attgacaata gccaaacagc gaagatgaca cctaggatcc caccttgtcc
    49801 agctgcaaga gcttcaccag taaataagtt tttcagtggt gcttccggat tcatccctgt
    49861 cagcatcaca acggccccga taactccacc aagagttggt gttgcaccaa aaacttgtgc
    49921 agcattgata cctgtataga taactaggta gctaaacatc ccgtttttca agatgttcat
    49981 cacatcgata tattgttgcc atgttgctgc atcaagtgtt ccggctgtga ctaagtttgc
    50041 taaaactgcg gcaatccctg ccacgatccc agtaccgaca aatgcaggaa tcattggcac
    50101 aaagatatta gaaatgtctt ttaataccga tttgatttta gatggttttt gtttcgattt
    50161 ttgtgccgct ttcatttctt gtgcccgttc gtttacacgg tcttttccag atgctgatga
    50221 atttgctgta gctgtactta cagaagaaag tttttctcct aatttcacgc ctgccaaatc
    50281 aaccatttgc tgtgctactt tgttcacttt cccaggtccg atgatgactt gaagctgttc
    50341 atcttctact acaccaagca ctcctggaat cgcttttaat tcagagacat ttacaagatc
    50401 atcattgatg atcttcatac gcacacgagt catgcagtgg acaatgcttt ctacattgcc
    50461 catgccgcct gcccctttat aaatttgttt tgccagttct tccaaagtca gttcgttttt
    50521 tgccatggtc ttttccccct cataaggtcc cttttacaaa tccgtttgct tcagctaatt
    50581 ttgcttgagc ttcctcttta tcactatttg tcaaaatcat gacgatagcc agcttaacat
    50641 tttgatctgc ttcttcaaag tacttttcag ctgtttcgta atcagcttct gtcgcttgca
    50701 tgatgattcg ttttgagcgt tcgattagtt tttcatttgt tggtttgaca tcgaccatca
    50761 agttattgaa tactttacct ataccaatca tactgatagt agaaatcata ttcaagataa
    50821 gtttttgtgc tgttcctgac ttcaatcgag tagagcctgt caaaaattct ggacctgcat
    50881 cgacttcgat tgggaaatcc gcgtgtttac tgatttcggc ttgtttattg caagaaatcg
    50941 ttcctgtgac agcaccgatc gattttgcgt aatctaatcc accgatcaca tatggtgtac
    51001 gaccgctagc tgcgatacca ataactatat cattttttgt caaattcagc ttgattagat
    51061 cttcttgccc tagctcataa gaatcttcgg ccccttcgac agctacagtc attgcttctt
    51121 ctccaccagc aattagacct atgaccattt ccggttctac accgaatgtc ggtacgcatt
    51181 cagcagcatc taacacacct agtcttccac ttgttccggc tcccatataa atcagacgtc
    51241 cacctttttt aaaggactca atcgtgttag caatcactgg ttcgatactt gtcagttctt
    51301 tttcaaccgc agcggccact ttctgatctt ctttgttcat tagttgaacg gcttgagaaa
    51361 tactcatctc atctaatcca aaagtttctt ggtttctgcg ttccgttgtt aggttctcta
    51421 aattgatttt gctcattttt atcttccttc ttttcttata aattgatagt tcataccagc
    51481 tttgatttgg ttgatcaatg gtagctcttc tttgataact ttagctacta cattcacttt
    51541 ttcatccgct ggcaaatcat atttggttaa ttggatctca cccatatagc gcaaatactt
    51601 ttcgttatct aaagtaatag accctttcat acgcgtagct gtatttcttg ctgggatagg
    51661 cggaatctct cgaaaacgtg catctacgct tcgtatgaca tctcttgcat cgtcttgccg
    51721 attcgtatgc ttccctaata tatattcgta aaattcttca tctacaggtt ccgtatggag
    51781 caagactgtc tgttcttttt ggaaagaagc aaattgctcc tgaacgtttt ctgacagacc
    51841 tgcatctccg atgtaaacaa tatccgtgtt acttgctaat agatctagag cgcctgcaag
    51901 aggatggatc cctcgatgtt cttccaaagt tggcaatcct tgatatagtg ggcctctcaa
    51961 ctctgtatct cctggtacaa aaccttggac aaggagacct tgaattttga gccattgatt
    52021 ttttttctga taccattctt tgtccagccc tgtttcggga cgtggataat aattatgcca
    52081 agcttcaatc tgtgagaaat cagcttctgc ttctttcaat tcatcgatat ctttaggcgt
    52141 gatcgtgcta gcattcaaac tgattttcaa ttgatgtgac cagtcggcaa tctgctgatt
    52201 ggaaatatga taatccattc ttaaacctgt tacaccaatt gctttcaaag gcgttagatc
    52261 ttctaatgaa aaacccgctc gttccaatgc ctctcctgaa atatcgacca tcaattctag
    52321 tttattcttt ttcgcttcgc ttcctagttc gatcaatcgc ttttgatatg ccgaaacatc
    52381 atcttctggt atatgcatgg aagtgaaaat accgacaaag ccattggatg ccatttggcg
    52441 gatatactgt ttcttctctt ctgacaaaga atcgttcata aatattgaaa agccaaacat
    52501 attatccctc tttttctgta tccgtcttca tttctatgac tagagtatat atctaaagaa
    52561 acaaaatttc aatactttta ttaaaataat taaatttcat ttctaaaggt tgagtttata
    52621 ttatcaaaag aattgtcata acctaaaaaa ggcactgcta agcaaaagca gtgccttgaa
    52681 atattttatt taaataacac aaaaacataa ttactcataa gaaaccaatt ctgtattatc
    52741 taccatgaat gtaaagaacc cctcattagg catcggatga aaataagcat tgatcactct
    52801 tatcaaggca ttatggcaaa ctaacagata gacattttct tcgtcttcga tgcattcttt
    52861 caataaagga acgatccgtg catacacatc taagactgac tccccatttg ggaatcgtac
    52921 agcgaacgct aaacgtgctt tttgaaagtt ctcatctttg cttggcagtc catcataatc
    52981 cccaaaatcc atttctacca atcgttcatc aacggtcagc ggcaagctga gacgatcagc
    53041 aactgcttgt gctgtatctc ttgctcgttg aagaggggaa tggatgattt tcgtgattgg
    53101 aacaggcaaa tctactacct tttctgctag tttctgtgcc tgcatctgtc ctttttcagt
    53161 cagcggcaca tctgcccgtc cgcaaactaa tccttgtgcg ttccaagttg tttctccgtg
    53221 tctcgttaca taaagcatgt gattctctcc ttcgttggca aagttcccct taaccatacc
    53281 ttgttgttct tgagattaca agggttagat tctctcaaga gaaataatct cagtagatac
    53341 tttatccagg aaggtttgat catgttccac tagtaacatg gcaggtttca cgttcaagat
    53401 cagttgttct aattgttctt gattgaagac atccaaataa ttcaatggtt catcccatgt
    53461 atatagttca gctggctgtg ataaagattt agccaattcc acttttttcc gttggcccat
    53521 actcatctgc tcgatcttgt tatgaaaaac atctctttcc atcccaagct ttcggaggtt
    53581 gttcaaaaat gcttggtagt ctacttggtt tttctctgca aattccgcca acgttcctcg
    53641 attgtcttca taattttggc ttgcataact aatgctcaga tgttttggct ggtatttttc
    53701 tcctataacc ttgccgttga atgcccccag aagataatgg ataatggatg actttcctgc
    53761 accgttagga cctgaaatcg ccacccgctg atgaggctcg attgtaaaat gaattggctc
    53821 gaataacaga ttttcatacc ctaattgaag atcttctacg cttaaaagtc gcttatggtg
    53881 agacgcttgg ctattcatcg tcaaagaatc gatatattcg atatctttta atagtttttc
    53941 tttctcgctg atctgggtct ccatccgatt cacgattgct ttggatcgtt tcatcgtccg
    54001 tgcagcatca gcacccactg ctcctttatt cactcgtcta gattcagtat cgatgaatcc
    54061 gacttgtttc tttgttttat ctccttctcg ggaacgagac cattcggctt tttcagctgc
    54121 tgttttcttt agcctactga cttctttttt caatttttca ttttgagcca tttcaaattc
    54181 atcacgaagt tttttctgtt cttcatagat agagaaattc ccttgataaa gttccagttg
    54241 acttttttcg attgctaaaa catggtccac tacttcatcg ataaatcccc ggtcatggct
    54301 gaccacgatg aagccttgtt ttttcttttt caaataagcc gctacttgtt ttctaccaga
    54361 gatatccaaa tgattcgttg gttcatcgat taacgggaaa tgagtgtcat ccacaaataa
    54421 aagtgccagt aggacttttg tcttctctcc ccccgatagt gtgctgaatt ctctccataa
    54481 gatttcagga tctgtttgca tcaattggag ctctctttcg atttcccata tctcaaaatc
    54541 cgtaatatca tttaacacgt aataggttaa acgttcttta tcttttgtct gttgcgggaa
    54601 ataggcaaat tcttgctgat ggattacttg cccttgataa ggtagtttgt tttgtagaat
    54661 attcagtaaa gttgtctttc ctcgaccgtt tcgtccgata agtcctagtt tccattgtgt
    54721 gtcaaaattt agatttgctt gttcaaataa taatgtgcct tggctgtcgt agccgaatgt
    54781 cagatttttt atttcgattt tagacatttt ccatcacctc gtaaaaaaag accagtccta
    54841 ttttcagaac tggctaaaga ggatgcaagg gttggataca cacaaaaaga cccatacatc
    54901 gaaaaaacca agccaatccc gaaaatctgc acactgcttc cttgtctcca caaggtacag
    54961 tctgaatgca gctttcatca atcgacttaa tttttcaatg cacggatccc tcactttgtc
    55021 ctatatttga aattacgata gcataaagaa aatgattttg tcaactactt cgggaggatg
    55081 gtgaaaaatc tgctaagtgt ctgtcctaag atttttgttt tttctaatgt ttgcctgtgt
    55141 tccattgctt cttctaaacc tattggaccc aaatgaatac ttaagacaat tgggagaaaa
    55201 tcagaaagtc cttctagctc ttggtctcta cttccgcaaa tagcgattat tggttttcga
    55261 tatttttttg caagcactgc tacaccgtat ggaacttttc cgtggatcgt ttgctgatcg
    55321 atccgccctt ctcctgtaat gatgacatct gcttgttcga tctttttttc caatccagtg
    55381 atttggctga ctaaatcgaa accagaaacc atcttcgctc ccaacaaaaa aagggctcca
    55441 ccaataccgc ctgctgctcc acttcctgga atatcatcta aatccacctc ccagaaatct
    55501 tttatttgct gagcaatctt tcctgcttgt ttatctaaga aagctagcgt cgtatctatt
    55561 cctccttttt gtcttccaaa ttgccaagca gctccttgtt gacctgtata tggattggta
    55621 acatctgcag cgataatcaa ttttacttta gaaacaagaa cgtttcttcg atcaaaatga
    55681 agttttttag tagataataa aggatttcct tctgtgtacc cttctaaaga agcacccaag
    55741 gattggagta atcctagacc accatcgcta cacccgcttc ctcctaaagt gacaataatt
    55801 tgttctactt cttgaagcag tgcatcttta ataagttctc ctaaggcata actggaagca
    55861 agtcggatag tttgatccgt tggtttatca agtagatcta accccaatgc ttgtgcagat
    55921 tcgatgactg cggtgttttt tccttgccaa gttgtcaata aatagctgat ttttcgctga
    55981 cggaataaga gatccatccc atcaatggta cgccatgttc ccctgagcgc gtaatagatt
    56041 gcttctgcag tattttctcc gccatctgca atcggcacat tgatttttct tcctttacga
    56101 tcttcccagc catttagaac tgcctgattt agttcaaatg aagaagcact tcctttaaat
    56161 gaatcaattg ccgccaaaat atccatctgt atccctcact ttcttcttaa gtaagacact
    56221 ttttttcatt tttttcaaac aaaacaatgc tattttcttc catttttcta tatactaaaa
    56281 gggaactcat tgaataaagg agagaaaaaa catgaaaatc gcaatagctg gggccggagc
    56341 aatgggcagc cgaatcggat tgatgctaca tcaaagcgga aatgaggtcc tcttgatcga
    56401 tcgttggcct gctcacattg aagccattcg tacaaatggg ttgattgctg actttaacgg
    56461 taaagaagtg gtcgcaaaac tgccaatcta ttcaccagaa gaaatcatcg aaagcaacga
    56521 acacgtggac ttgatcgtag cattaacaaa agccaatcaa ttagatgata tgttttgttc
    56581 gatccaatcc attattactg acaatactta tgttttgtgt ttactaaatg gattaggtca
    56641 tgaagatgta ctggaaaaat atgtgccaaa gaaaaatatc ttatttggga tcacgatgtg
    56701 gacagctggt ctagctggtc ccggaaaagt cacattgtta ggtgatggag aaatcgaact
    56761 agagaacttg gaaccagaag gcgaagcatt tacaaaaaaa gttgtagaag tctttcaaga
    56821 agcaaattta aatccgatct atagccataa cgttcgttat tccatttggc gaaaagcttg
    56881 tgtaaatgga acattgaatg gtttatgcac gattttggat tgtaatattg ctgaactagg
    56941 cgcgcaaaaa gcggcagaga gcatggttcg aacaatcgta tctgaatttg cttctatcgc
    57001 agcaaaagaa ggaatcatac tagatcaaga agaagtgtac caacatattg cttctactta
    57061 tgatcctgac aacatcgggt tgcattaccc atctatgtac caagacctga tcaaaaacca
    57121 tcgtttgact gaaatcgact acatcaatgg tgccatttgg cgaaaaggac aaaaatatga
    57181 tattgctaca ccatattgtg cctttttgac ccaattagtc catgcaaaag aaggaatcct
    57241 tggcgcagaa taaaaggctc tctgatgaga atcagctgaa tcagcagctg gttctctttt
    57301 tttaagccca ataaaagatc aaaaagaaaa cacaactctc atgatgccat aagaattgtg
    57361 cttgtttgtt tcagtacata atctcttcaa aatgtaggtt ttttatttgt tttcaacaga
    57421 ggagaggtca tattcgtatt gctcataaat agtggaatcg ttagtgataa atcgttttgc
    57481 ccaattttct gcatcataaa attccaatag ctctgatagg attggcttaa atggtgtatg
    57541 cagaaattcc ggtaattcat ctaaatcacg ttctcgaagc aaaaaaatat tttcaggacg
    57601 ataaaaatga taatgagcaa tcgcttgatt atccgtcact agtttctttt gaaaatacat
    57661 ggattcaaat gtccgcaaag tcactccttg ctgaccctcc cgcaacagtt ccagaatccc
    57721 gcgcgattcc cctgtttttt tcaggtactc ttcataagta agatatccag gttgttcatt
    57781 ccctcgatgt ggaagaatga aatagttggt cgtaacatct ctttttataa attcctgctt
    57841 atagctttct gctcgttctt ttctcccatt attcgttgca ccaaaaaata gatccgtctt
    57901 tggttcgcta gaagaaaaat tcatatgttt cgaataaaat gaactattat gtttcagccc
    57961 aagttctttt gcttccaaaa aatcaaaatg ataaatctca tcgattgccg gttcatcttg
    58021 tgcctgcttc agcatcgcct cgtgttctgc tttgaagtga ttccaaaaaa ataacacaat
    58081 ttttgcattg gtaaatgggc gcacgcgtcg agccatctcc atatcaaatc cataatctaa
    58141 aaaaacgatc agctgataat ttgccaactg ttgtctagct gatttaatga attccgactg
    58201 tagtaaatct tttccggacc aaacatctaa agagcggatg gaaggcaaaa aataaagtga
    58261 tttatttttt gcaactagca gtacgtcatt tttgttcatc ttttccgcct cactttctct
    58321 gtcacttttt cctatgatag cctgtttaaa tagtaaaaag ccagtaaaac gtttcctata
    58381 tttgtatgaa aatttaacat ttgtatgaaa atatgatgaa ataaatcatt cttctattaa
    58441 aattcgccta aaagttttct gaatagttgt aattgtctga aaatttgtta tcattagtca
    58501 ttgttaaata ttgctattta ttcttaatat aaattttgat tgtgaattta aaagaaagag
    58561 gtcatttatg gaagataaaa aattgaaaaa acaacttagc tccagacata tcaccatgtt
    58621 agctttaggt ggagcaatcg gtgcaggtct atttaaagga agtggcgaag cgattgggat
    58681 cgccggccct tctgtattga ttgctttttt gattggtggg ctgattttat atatcgtgat
    58741 gaaaggtctg ggaaaaatcg ttttaagcgg tggcgacacg catcacggat tatctggttt
    58801 gatacgccct tatctaggtg ctcattctgc tgattttact gattgggtct actggtctat
    58861 gtggattatc aatatcatcg ctgaagctgt tgcagctgct tcctttttgc agttgtggtt
    58921 cccgcacatt ccagcctggg tatttgtttt tatgttggct gttttaacta cgatcatcaa
    58981 tctttattcg gtacggttat tcgcagaaac agaatattgg ctggcttttg ctaaaatcag
    59041 tgtcattatc ttactgatca tttttgctac ttatcttgtc ggtcagcaaa tgttgggcac
    59101 aggcgttttc cctacccttc agcaattgac tgaccatggt gggtttactc ctcatggtat
    59161 gaagggaatc atcagttctt tgcttgtagt tatctattct tacggcggtt ctgaactgat
    59221 tgctatcacg gtaagcgaag cagatgatcc taaacgtgcg attcctaaag caatcaaagg
    59281 tgtcatggga agaatcattt ccttttacat cattcctttg tttttgcttt tgattctttt
    59341 tccttggaat actttagcag gcacgaatgt cagtcctttt gtgatggtat ttgaaaaaat
    59401 gaatatccca tttgcagcgg atatcgtcaa ttttgtgatt gtccttgctt tgttctcatc
    59461 tatcaattcg ggtgtgtatg catcttcaag aattttgttc ttccgtttga aggatcgaaa
    59521 aggttcctct agaaaactag ctgttttaaa caagcatcaa gtaccgcaac gtgctgtttt
    59581 attttgtacc agtgttcttt atcttggtgt agctctttct tattttgtcg gtgacaaatt
    59641 atttggctat ctggcaggtt ccctttcgta tacagtatta ttgatctgga tcattatcag
    59701 tgcggcatcc tttgtactgg cactaagaaa aggaacatta tgggataaag gaatcagctt
    59761 ttttgcatta gctgtcttag gattgatttt ccttggtatc ctgttcacga actccattgg
    59821 ggtaactgta ttgactggcc tgctttacct gttcatttat ttcagttatc aaaaaaagaa
    59881 tgatgcgttc gtattgacga atgaatgaaa tcgatagaaa attttatttt atttatgtgg
    59941 aatagatgga gctattgaag aaaggagttg tgacaaaagt cttgttgcaa ctcctttttc
    60001 cgaataaaca gtctcatgac ctcttggtaa acggtgttca aaaaccctct gtgcaaaata
    60061 attgattgtt ctccatgctc atgacatgat agaagaaagg aatgaaaaaa aggagtttgt
    60121 tttaaatgaa aaagtattgg aagtttttgt tgatgatcgg tgtttcaacg gtagttatgt
    60181 ttggtatgat gtacttgaat gtctatgcgc tcgatcatat cttcttcagt cgtacacgag
    60241 tattcatggc gttgatgatg ggtgcagtca tggcagtcat catgctgttg ttcatgtgga
    60301 aaatgtatga aaacaaaaag ataaatgtta tgatcttagc cgctagtctt cttgtttttg
    60361 ccggttcctt gtttatggta cgaagccaaa ctactgtcaa cgacacagct tggatgaagg
    60421 ccatgattcc tcatcattct atcgctatcc tggcaagtaa acgagctgat ttaaaagatc
    60481 ctgaagtaaa aaaattagcg gaagaaatca ttcaggcaca agaagaagag atcgacaaaa
    60541 tgaaaaaaat gatcaaagaa atggaatgac cattatccgt ccaaataaaa aaagaggatg
    60601 ttccagaagt tatcagcttc aagaaataag aagaagctac ttctggaacg ccgtgaataa
    60661 ggggttgtga aaaagctgcc ctgcatcaag taataagaac agccaaacaa aaatagcttc
    60721 tcctattttt cgtttggtta ggcttattac cgcagatgca agcttttgaa caccgtttat
    60781 taagaggttg taaaatcagc gttctgacct gagtattaga gagacaaatg gaaaaatagt
    60841 tcctcatatt ttgccatttt ttgagctaat accgagggtc agctgattga acaccgttta
    60901 tcgagaggtt gtgaaaaagt cgcttagttt cgcgtattaa gaaaaatttt gtgaaatggc
    60961 tttccacatt tttgtataaa tttggcttaa taccgagaaa ctggcttttg aataccgttt
    61021 attcgtaaaa gggattgtga caaaagtctt gtcacaatcc ctttttatgt ttttattgtt
    61081 ctaattgcgc tgtcttgctt actgcttctt gcggtttcac aaattttttt gagattcccc
    61141 aacctgtgaa ggcactgacc atcgaaaaca caggagaaag caaactgaaa aatacgaatg
    61201 gcaagtaagt gagtgtagga ataccaaatg tacttgctac gaaacttccg gcaattcccc
    61261 aaggcactaa gtagttgatt actgttccac catcttctag cgttctacct aatacagttg
    61321 gatcaaatcc tgctttttca taagtttctt taaatgcatt accaggcaga ataacagata
    61381 ggaattgttc accaataaaa atatttactc cgatacttgt catcaatgtt gtaaaaatca
    61441 aatttggtgt gttcgtcatc tttcttgata ctcgttccat cacggttgag atcaagccaa
    61501 attcgatcaa caatccacct aatgacaaag ttagaataat caatgacaca gtaggcatca
    61561 tactttcgat tcctccgcgg ctaagcagtg aatcgatttg ttggtttcca gtatttgaaa
    61621 caaaccctga ttcgatgacc ccagccaact tcgttaagga agtttgtggg ttttgaataa
    61681 aaatcattcc tactgctaca gcgatattca acaaaatcgt taaaattgcc ggcacctttt
    61741 tccatgcaca cagtaacatt aaaaataaag gaatcagtga ccagattgaa atagtaaagt
    61801 ttgcttctag aacatcagtc acttcacgaa tagatgaaag acttgcggct ttacttgtgt
    61861 gtcctaaaat catgaataaa aagaaagaaa caacaaatgc cgggacagtt gaccacatca
    61921 tatgcttgat gtgtttgaac aaatcggcat caacaatagc tgctgctaga tttgttgatt
    61981 cggataatgg agacatctta tctccaaaaa cggcacctga aacaatagct ccaacaacca
    62041 gcgctggatt taagcctagt gttgtcccaa taccgaaaaa tgcaatccca atcgttgaca
    62101 taacagtaaa ggcactccct acagcgctac ctacgattgc acacacaaca aatacagaag
    62161 ggacaaacca ctggacactg atcaatttga acccaatgac cattagagtt ggaatgatcc
    62221 ctgcttgtat ccatacagca atcaatgaac cgactaaaat aaaaataaag attgggatga
    62281 ttcctggttt gatgccattg atgattcctt cgtgcagcca atctgtcggg tatcttttta
    62341 tcactgcata caacatgacc attccgatcg ctgctaagat cgtgatatta ggtgagacct
    62401 ttaatccgac aacgccaatc gcaatgccta aaacaataac cattaataca agtgttgctt
    62461 ctaaaaaact tggtgttcct ttttttccca tgataatctc tcctttttta gttgttctta
    62521 tgttctaaaa aagttaagat atcggattac tccgccgcaa ttcatccgcg attcctcctt
    62581 tataaaaata aaaagacctg ttttaaacta aggacgaatg actccgcggt accaccttaa
    62641 ttaaaaacag atctgttttt cactttcaaa cttttattca aatgtatgga ctttccatgt
    62701 aattcgtcaa ttaggcttga tcggttcaca gcgaccaccg actttctgaa caaaacacct
    62761 aaccgctact gcgtgaaaag ttgacttgta cgaagtttac cttccataag atcatttgtc
    62821 aatgaaaaag aaaatatttt ttcatttttc tgaaaattat ttttataact ttcacacaca
    62881 agtttatttt ttactagaaa gtagcgccct cttgtttttt tcttgctact atttcttgat
    62941 gttcttgctt gattctttct aatagctttg gctctttcaa cagatcaaga gctgtattgg
    63001 cgagtagttc tgctgcaatc gcaatagaag caaggccttt gtcactttta gcagcagctt
    63061 tgaattcttc tgtgtgtccg gcaatatatg tctcagagat tgctaccgta ggctggattg
    63121 ttggtatgac ttggctgaca tttcctacat cagaagaacc gagacttgtt ctttgttccg
    63181 tttctatttc ttcatctgga acacctgctt cttttgcatg ctggaagaat aagtcatcaa
    63241 aagaaggagt aacgatgaca tcatcaactg cattttggaa caatccaaac tcaaaagtcg
    63301 tgcccgtact tatcgcagca cctcgtacga tattttccac tttttcatat actttatcta
    63361 atgtttgccg atttcctgca cgtaaataga aacgtccagc agcgtaatct ggaatgacgt
    63421 tggcagctac tcctccgtct gtaatgatcc catggatatt gacatctttt ggcaattgca
    63481 agcgtagagc gttgattcca ttaaacactt ggatcaatgc ttccaaagca tttactcctt
    63541 tttcaggtgc tgctgctgca tgggaagcga caccgtaaaa tttgatgtcg actggatcat
    63601 tcgctaaaga ttctgtcgtt ttcccataac gaaaagctgg atggacgcat aacgctacat
    63661 cgacatcctc aaaaaaacct tctcgaacaa agctgccttt agctgatccg ttctcgcccc
    63721 cttcttcccc tggggtgcca tatacacgga tttctccacc agtttcatca atgaattgct
    63781 tcactatgct ggcggctaat acggagtaag taccaaacaa attatgcccg catgcatgac
    63841 caatacctgg taaagcatcg aattctgcca aaaaggcaat tgtgggaccg ggtttattgg
    63901 aaatataacg tgcatcaaag gctgttcggt gtccagctac ttctttttct acccaaaacc
    63961 tttctttttc taattgttta atcaacgctg ctgaggaata aaactcatag ttacttactt
    64021 caggatgttc gtggatatct agagcaagtg cttgatattc tggtaaacga tattggatta
    64081 gatccttcaa ttggtttgtt gagattgttg ttgtcataaa aaattcctcc tatgctagtc
    64141 atttttctta ttttctcccg aatgtttccc atgcaggcac gcttgatcct tttgatgttt
    64201 tctcgattac tttgactgtg tcttctgttt gataagcgtc tactattttt tgatacgttt
    64261 tattgttttc atcttctttt cttgcaacga tgatattcac ataaggacgt gcatggtcat
    64321 ccactggttc taagaagatg gcatcagaag caggattaaa tccagcatct acagctacac
    64381 cgctattgat cactgaaatg tctacatctg acaaagcacg tgctgtttgg gaagcatcta
    64441 attcagaaat gtctagattt tttctattct cggtaatatc agaaacagtg ggcgcttgtc
    64501 cttttgcctt atccactttg atcaatccag cagtttgcaa taacagaaga gctctgccgc
    64561 catttgtaac atcattaggg attgctactt ttgctccgtc tttgatatca gctacatcct
    64621 tcaaagtgtt agaataaatt cccagcggtg cattgacagt atttccgatc gatactaaat
    64681 cagttccgtg ttctttattg taattgtcta aaaagaattg atgttggaaa gaattcaaat
    64741 caatcgatcc atctgctaaa gccacatttg gttgattgta atctgtaaat ttcacgtatt
    64801 ccaaattaat gttctccttt ttcaaacggt ctttcacgtt gtcccataca tctgtatctt
    64861 ctccaataat ccctagtttt acgatagttt cttttttgtc agttgtatct gcttttcctt
    64921 ggctacaccc tgccaaccaa ataattgcta caaatgtaat gactacaaaa cgaataattt
    64981 ttttcatttt ttctcctcca ctttcaatgt gtaatttttt taatcagttg attactgata
    65041 aattgactta aaaaaactaa gattagaatc aataatgtag ccacaatcgt tacatctgtt
    65101 tggaatcgat tataccctct cgatatagca agattcccta atccgcctgc acctactgct
    65161 ccagccatgg tagtcagtcc gatcacgttg atgatagtca acgccgagac ccgaatgata
    65221 ccaggcaaac cttctaacaa atacacacgt aaaatgatac cgattggact ggttcccatc
    65281 gcttctgccg cttcgatcac cccaggatca acttctagca gtgcgttctc gatttgacga
    65341 gcaaaaaacg gaacaactcc aaaaatcaat ggcactaaag ctgctttcgc accaatcgtc
    65401 gttccaacta agaaacgagt agtcaatgct aaaagagaaa gcaagataat gaaagggatg
    65461 gaacgaatga tattgatcag cttatcaagt atttcaaata gcagacggtt agctagtaat
    65521 ccattagggc gtgtcactac taacgtcaca cctaagatga ttccaatcat cccgccaatg
    65581 aatgctgtcc aaaaagacat atatagtgtt tgcttcgtcg cttcgacaaa ttcatccgga
    65641 atcaaataaa cattgggcaa atatgtttta aagaaatcag tcattttatc ttcctccttc
    65701 gatcactttg atacgactat ctaaatcgtt tttttgtaaa atatttattt ttacaccaga
    65761 tttgatcaaa taatccaatg cttttttccg ctgtttggtt tctccagcta aagtaacgat
    65821 caaactccct aacggcgttt cttgtaataa ctctacattg ccatataaaa tattcgctgt
    65881 tacttgatat ttgctgtaca gatgagcgat tagaggttcg ttcgtctgat ttccaatata
    65941 acttaattcc accagccatt tattggcgat ctgatcggca aaagcagaat gggcaatgat
    66001 cgtttctaat gcttggtcaa gatgagtagc tgttcgaata aaatctttgg tcaattcttc
    66061 ttcaggattg ctgaagatct ggatgctgct acctttttca acgattcttc catcttccat
    66121 aaccgctacc ttgttgcaga tttctttgac aacttgcatc tcatgagtga tcaggacgat
    66181 tgttaaacct aactggcgat tcaatttttt taacaatgca aggatctgaa gggtcgtttt
    66241 cggatcaagt gcactggttg cttcatcaca taataatacc ttaggttcat tcgccaaagc
    66301 tcgtgcgatt gctactcgtt gtttttgtcc ccccgacagt tgtttaggat aagcagatgc
    66361 tttttcttcc aaaccgacaa gttccagtag ttcattcact ttttgacgac gttcctgttt
    66421 cgattttcca gaatacttta aagaaaaatc aacgttgtca aaaatagttc ggctttccat
    66481 tagattgaaa tgctgaaaga tcataccgat ggcctttctt tcttttcgca attctttggg
    66541 agagagctga ctcagattcg tctgattgac aatgacttct cctgctgttg gtttctgcag
    66601 caaattcatc acacgaacta acgtactttt ccctgcacct gagtaaccga cgattccata
    66661 aacatcgcct ttttcaatcg ttaaatctac agaatctact gcctgtatct ctttttcttt
    66721 ttgtttgaaa catacagaaa catctcgcaa ttcaatcatt aaaattcctc cttatctaaa
    66781 aaaagcgtcc tcaagaaaat gaaaatgtca ttttcttgag gacgcttttt tgcgtgttac
    66841 cacctcatgt tcaagctgtg ttcacacaca actcctcttc aagtacgatc atactctagc
    66901 aagatatcgg tcgcagccgt catcgcctta catctatgct cgggatgaat tgctcgatgg
    66961 ccattttcca atagtcgttc cttgcttctt ctcagctacc gaagctctct gtcaaaggtt
    67021 cttctatcgt acttaccatt tcaatgcaaa taatattcaa aaaaaaagtc cttttactaa
    67081 cataaactat gtcagtaaaa ggacgacgta tcgcggtacc accttttttc ggaagtttcc
    67141 ttccctcaag cccaacatct caaaaagaga gattggcgtt cgttttaacg gtcgaacagt
    67201 tccgaatttg cctacttatc gacaaattat tctccaaggc cattttcagc ttttcttact
    67261 tgactcttct caccagccag agcctctctg caaagtttcc aaacttactt tccttttcaa
    67321 cgaaatcgat attttgttgt tgggattaat ctacatgagg aaaactgaaa agtcaagtac
    67381 gaaattcaaa ttattttttc tatatttatt tttctgtttt tttatttaac atttcaataa
    67441 cagttcgaag atcttttcct tccgcaagaa gtttcaattt ttccttttct tcgacggtat
    67501 aatttcggac aaaactggaa tcaggtatca aaacaacgcc taatcctgct cgtgaagcag
    67561 ctgttgctcc tactaaagaa tcttccactg caaaggctga actagcttct aatcctgttc
    67621 gttccaaagc tgtcagataa ggaatcggtg aaggcttgtt ctccgacact tcgtctccga
    67681 atgtaaacgt gtccaagtaa tcagctaatt taaagtgatc caagatatct gttgctcttt
    67741 tcgtaacagt ggtcgtagca atgcctagtt tcatatgacg ttcttttaag tctgccaata
    67801 tttcttttgc atatggtttt aagtggatgg ctccgcttgt cagctgttga taaaaatatt
    67861 cttcacgctt cgctcggact tcctgtactt tttcagggct acctgctgct ttcactaaaa
    67921 tatcaaaact ttgtttccag ttttttccgc cccaagcgtc aacgatttct tttgggatat
    67981 ggatatcaaa ttgctgaaat ccccaaagcc agccatcacg atacgttttt tctgtgtcaa
    68041 ctaacaaacc atctaaatca aatattgcac ctttcatcta tacattaatc ctttccaaaa
    68101 caatacacat agtttagcac gactggtcca aaaagaacag aagcaaagaa ccagccttct
    68161 cgtttataag agttcttgga atcgacggat atagattttc ttcacagctg tcacaagcgc
    68221 cagataagcg atgactgtag caaataacca taaccagtaa ttgccaggaa gtggcatcaa
    68281 gcctaattca gcaccaaaac ttgtaaaagg aaggacagaa ccgacaataa ttcctaatgt
    68341 agtgataaca aacatggcaa atgttgcgtt gctttggata aatggtactt tcggtgtacg
    68401 cagtgcatgg agcactaagg tttgtgtcca tagtgattcg acaaaccatc ctgcatggaa
    68461 taatgcgatg aaaatcactc gctgtgaact atccaatgta tggaaacttc cgcccactac
    68521 agcaggacaa ataacaaaat acatcaacaa gtaagtagta atatcaaata tcgaacttgt
    68581 tggaccaaag taaaccatga attccccaat actagatgca ttccattttt ttggctcgtc
    68641 caaatattct ttatccatcc gatcccatgg caaggaaatg caggaaacat catagatcag
    68701 attcaaaaac aaaagttgta aaggcagcat tggtaaaaat ggcaggaacg tacttgcaat
    68761 cagcacggaa aacatattgc caaaattaga acttgctgta gccttgatat atttcatgat
    68821 attcccaaaa gtttctcgtc cagaaagaag gcctctttcc aaaatcgtca aatctttttc
    68881 taaaaggatc acgtctgcag attctttcgc aatatccaca gctgtatcta cagaaatacc
    68941 gatatcagcc actttcattg caggagcgtc attgatacca tctcctaaaa atccgacagt
    69001 atgaccattt ttacgtaaag ctgtcgtcaa acgtgctttt tgctgaggat tcaattttac
    69061 gaatacttca tggtcttcag ctacttgtgc taattcatga tcactcattt ccgagatttt
    69121 ctcaccggta accaattcat tgatctctag acctacttga cggcacactg aacgtgtaac
    69181 taattcgttg tccccagtca gaacttttac agctacacca tgttctttca gagctttcaa
    69241 cgcttgtttc gttgtttctt ttggtggatc aaggaaagca agataaccaa tcaaaaccat
    69301 gtctgactca tctttgactg aaaattctcc aacgacgctt ggattggtct tttgtgcgac
    69361 accaatcact cgtaagccat cttcattaag tgactgtact tttgcaagta cggcttcttt
    69421 tcgttctttt gttaatggtg taacagtacc gccaagatca ataaatttag aaatgttcag
    69481 catttcttca atcgcgcctt ttgtgatcat ttgtgtttta ccagctggat cagcaaccac
    69541 aacactcata cgacgacgtt caaaatcaaa aggaatttca tccacttttt gataattgat
    69601 cttatctgta tctaaagttt ctgttgcttc atcaatgata gccacatcta acaaattttt
    69661 cagccctgtt tgatagtagc tatttagata cgcatggcgc aaaacacgat catcttcatt
    69721 tccttcacaa tccatatgat attcaagaat gattttatct tgtgtcaatg tccccgtttt
    69781 atccgtacat aacacatcga ttgcaccaaa gttctggatg gcattcaaat ttttgatcac
    69841 tgttcctttt ttggccatca cacttgctcc tttgaccaaa ttcgtcgtaa cgatcattgg
    69901 aagcatttca ggtgttagac caactgctac agatagtcca aaaagaaaag cttctagcca
    69961 atcccctttt gtaagcccat tgatgacgat aactgctgga gccatcaatg acataaattt
    70021 gatcagtagc atggatgttt tatggatgcc taattcaaag cttgattgaa ttggtttttc
    70081 agatagcgtt tctgctacat gaccaaatag tgtttcattt ccaacagaaa caatgattcc
    70141 ttccgcactt ccactgatga cattacttcc cataaagaca agattttcgt aacttgtttc
    70201 gctttgctga tcgtcttcac atactttcgc atgcttttct actggatagc tttcccctgt
    70261 caatgctgct tgggaaatga ataaatcctt tgcggacaat aaacgcatat ccgctggtat
    70321 catatcccct gctgaaagcc gaacaagatc tccacagacg atttcttcca taggaatctc
    70381 tgtgtacttt ccattacgtt ttacagctgc cgttactttc acaagtgatt tcagtttttc
    70441 agccgcttga ttggaacgta cggactgaat caacgtcatt gtcccgctga cgataaccat
    70501 agtgaagata atggctacac cgaaaagatc tttatctcca ggagctgcta atacatattc
    70561 cgtgatgaat gaaataattc ctaatgcgat caacactaat gtaaatggcg tgatgtatgc
    70621 tttcaatatt tctacaatga atggtgtttt tttgccatat gaaatcgcat tttcaccgta
    70681 attttctctt gaacgttcga tttgttcttc tgtcaatcca tttgctgatg catggaatag
    70741 ttcaaataat tcttgtttcg tttttccagc aaaagctgca tagatttttt gtgtatcttt
    70801 ctttttttcc atctcaatct ctcctttgtg tccaaaagac agctgttctc gctgcttttg
    70861 gtctttagct attcatcgga tcgatagaga aaaaactgaa aggggctgag aacagataga
    70921 taatagccct ggcctttcct ctagatcctt gtttttcgaa tgactgtcat ccatttgttc
    70981 tcatctcctt tactaagtaa ttttaattca tgacttccaa tcatttttcg aatagttatt
    71041 tttccaatta gaccacctcc tttttatttt tcaccgatgg ggatttgcgg caacaaaaaa
    71101 acctctcgcc aaatactcag cgaaaggttt gaaaaagcat attaaaaaaa gcttctcaga
    71161 tctcatcgtt gagttttgac actgtacaac gtaaaaagca gatacctgct tttagctacc
    71221 ttatgaaaag ccttatctcg acaattcctg ttctacccat tggcgtctct cgacgttttt
    71281 gggcagtagc ctatgtttgt ataggagcct cacctaacaa atcttattcg gttttccttc
    71341 gttaatataa caactaaaaa agaggatgtc aaatcaattt tccatgtttt tcatactttt
    71401 tttaaaatac acacgttata ttcctgtctc acgcggtttt atttgtctca ttttccacct
    71461 cccgctataa tagaaatata gtaggaaacg aggtgctgtt atggaaagaa cacttgttat
    71521 tatcaagcca gacggcgtga gacgccattt agtcggaagc atcatccaac gttttgaagc
    71581 aaagggactg gccattgctg aaatgaagtt tgaaactatg actcctgaat tagcaaaaga
    71641 acattatcag cacttgactg agcgcagttt ttttgatgaa ctgatcgatt acatgacttc
    71701 cggtcctgtc gtttatcttg tgctggtcgg cgaggaagtg atcgatatcg tccgtaagat
    71761 ggttggagca acaaaagctg ccgatgctgt tccaggtacg atccgaggag actacgcgct
    71821 gccggggact gaaaacatca ttcacgcttc tgattcccgt gatgctgcgg taaaagagat
    71881 cgcccgcttt ttcccgccat tcgatataaa caaaccgtct gaaaaagcgg cgaagtgata
    71941 caaattctaa actgctggtc tagttgctgg gttcagcaat ggccagcagt tttttctcta
    72001 aggacctccg ttgattcttt cctaacatat attcgagcac tgtgaagaat tcttggtagt
    72061 ctttcgtctt ttcttcccct aaagcagcag aaatcaatct ttgataataa tctgccatca
    72121 atgtttcata gatcactgta cttctttctt tatgtgctaa gctccgtttc atatcttcat
    72181 tcacactacg tgcctttttc aaccgaccat tttctatata aaacgaagtc aggttcatta
    72241 aaaaagtaaa gagatactgc tcatgattgt tcaactgtgc taatcgtcgc attctttttt
    72301 tgattttcaa ataattgttc tcaatgaacc gatcactgaa tatccaagga aaatcagcta
    72361 gaatagatag ttcaaattga ccatagtctg gtatctgcat caagtattct tcaatgattc
    72421 ggcattcata acgtaaaaat tcaaattctg tctcagatac ttctgttttt tcgaccatgt
    72481 gaagattcaa aaccaaataa tacagatctt ctgttttctt aaaatgccta cggttattgc
    72541 tgatctcttt ataaggatca aagctgtctt cttgtacccg atctagcatt cgtctttttt
    72601 tctttaactt tattggttca tccagctggt ttcgcatcca ttcatattct gcaagtgtca
    72661 tgttcattcg ctctaaaaat gatctcaatg ttacaaatga catttttttc actgaatttg
    72721 tctcaatccc gactagagaa gtcttaaaac agattccttc cgctaattgg gactgtgtca
    72781 acttcttgtt gactcgaagt ttcttgatca attcaccatc atacataata gttctccttt
    72841 ttaatttaaa tttttccaca ttcaataaat agatacgtta aaaaggaaaa atacacgaaa
    72901 aaaataaaaa aatgtttttt tcataaaaaa taccaacctt caagcattag aatgttgtct
    72961 aactactgaa ggttggtttg attgtttatt gattattttt ttgctgttca acgtctgttc
    73021 ttgttactgg atataagttg gttgttgttt tattaccatt ttttgaaaag acacttgtcg
    73081 aatgttcgcc tttttgcttt tcgattgctt cattacgttc cgcttcattt ttatagtcat
    73141 attttttcgg atcaatcgtc tcaaatccgt ttggcacata aaaccgcaag agattttcct
    73201 gattcagctt atctgaaaga cttaacgcag tcttgacttt ttcctgatct ttggagattt
    73261 cctcatcgat tcctgctgct ttcgtatcga tgatttctcc tgtctgatta ttgtaagctt
    73321 ttcctcctag gacggtgtat ttgggtgtca caaagttccc atttcgaaaa gcaactactt
    73381 gatcatgctg agaagataat aagtctgtac caaaatgaag ataatcttta tcatcgattc
    73441 ccaatagatg tagtaatgtc ggcaaaacat cgatttctcc accgtattca tgattgactg
    73501 tccctttagt ataaccaggc atatggatca tcaatggcac tcgttgcatc tgtgcgttgt
    73561 caaattcatc ccatgtatct ggatctttac caagagcgct tgctaaatct ttattatctg
    73621 tattcgagat cccaaaatga tccccgtaaa tcacaaagat tgaattttgg taaataccag
    73681 attttttcaa atacgtaaaa aattgttcca atgattgatc cagataatgt gccgttcgaa
    73741 cataattatc tactgtgcta ttgccagtat tcaaagatgg aaagtcgaaa ttcttgtcat
    73801 ccgtataata aggcgtgtgg ttggttacgg aaagaaattt cgtgtaaaac ggctgttgta
    73861 gatgctcaag atattgggca gattctctga ataaatcttt atctaatatc ccataaccta
    73921 acgtttcgtc ggattcatca aaataagatc gatcaaagaa attgtcataa cctaagtttt
    73981 tatatacatg atctctattc caaaaactag ctacattccc atggaaaaca gcgcttgtat
    74041 agccttgctt ttgtccaagg atcgctggtg cggcttgaaa gacattgtct gacccaagtt
    74101 ctgtgaataa tgagccttga ggtaaaccaa atgtgccagt ttccaacata ttttcagcat
    74161 cacttgtctt tccctgtcct acttcatgaa agaaattgtc aaagctgatc gttgcttgat
    74221 tctggaaaat actatttaaa aacggtgtga cttcttgtcc gtctaccttc atattaatca
    74281 aaaactgctg gaaactttct aaatggagaa cgatcacatt tttcccttta gcgatcccaa
    74341 atgtttcggg atttggctct gcataatgtt tctttgtata atctaaaact tcatcaatcc
    74401 cattactgct tgcatgcgcg cgaacactgc tagtcattcc tgttttgatt ccgtcataaa
    74461 tagtatacgc atcgattcct agatatttga caatatatga acggtcaaat gttcgctgca
    74521 gcaattgtgg acggttggct tcgctcagcg ctagatttcc tgaaaaaata agccctgaaa
    74581 gacaggtaac agcgatagcc atcggtttgc cgacagggtt gcttttgata ggtattttct
    74641 tgaccttcaa ccaaaccagt agcccaatga acacagcgat atccagccag tagatgacat
    74701 catggggctt catcaaagaa aaggagcttc ctgacaatcc ttgcgacact ttcgaaaagc
    74761 ctaatacaga tttgactgta atgaaatctg tgaattcccg ataaaaaata atattcaagt
    74821 acaacaaaag cgtatttaaa atatcaatga tcaaaagaat agctaacgct ggctttggct
    74881 ttttaatata caaagaaagg ccaaaaaaca ataaggtagt cgcaattggg ttgatccaca
    74941 agataaagta ctggatcgta ccttccacac ctaatgaaaa atctgcatag taggcaatga
    75001 tcgtcttcag ccagaaaaag aaaacagctg tcaaaacgat tgctaaacga ttttggcaca
    75061 attttttaag ttgtgtcacg tatatcactt ctctctattc acattttttc ttattttgct
    75121 cctgaaaaat gaaaatttcc taaaaaagtt gttatcttaa gattttttta ttctttctaa
    75181 agattattcc gtccactttt cacagcataa aaccgctgcg atgtaagcga ttaaactaat
    75241 gaagagataa aagtcttgtg gtgaccgctt cactccacgg tattcaaaag ccagtttctc
    75301 ggtattcgta caaaaaaaga ggctgtgaca aaattcttgt cgcagctcct tatttcttga
    75361 tgcaggacag ctttttcaca acttcttcct taaacggtgt tctcctgcca actcctcggc
    75421 cacaagtcac aatatttata aaacatgaga agctgttttt tgaaattgtt ccttgtggct
    75481 cagagttaaa cggcaggctc aagacctctt gatacacggt gttcaatcag ctgaccctcg
    75541 gtattagatt atgtcaggac gctaatttta caacctcttg gtaaacggtg ttcaaaagct
    75601 tgcatctgcg gtaataagcc caaccaaacg aaaaatataa ggagctattt tttgaaattg
    75661 ctccttgtgg ctcagagtta aacggcaggc tcaagacctc ttgatacacg gtgttcaatc
    75721 agctgacctt cggtattagc tcaaaaaacg gcaaaatatg aggaactatt ttgccgtttg
    75781 tttctctaat actcaggtca aaacgctgat ttcacaacct ctgtttttca ttgtccatag
    75841 gattgatatt gtccttgtga tgactcattc gtaataattg aggcttgatt ttcttcactt
    75901 cccgttatcg tttgtccact gccagaatct gaaggacttg ttcctgtcga ctcgtttttt
    75961 tgagaagaga cagttgtttt cttgacttgt tcatttgtct ttttccattc cgctaaacga
    76021 tcggcgacca cttttttgtc tactttttca gaataagcgg tgatcacttt tccatttttc
    76081 aaacgggcta ctttgtcgcc atcatgagtc aaaagcaaaa cggtcaaatg cttgtcttta
    76141 aattgatatt gtctgagaga aatcgcacta agtacgtcat cgatattaat aaattgattt
    76201 tcttcttttc cttttggcaa ataaatgtcc aattcgcctt tttcgtaatt ggcagttatt
    76261 ttatactccg cccaccaata agttgagaat tcttttttga aattatctac tgattctttg
    76321 cttgctacga ttttttctgt ttctttctga gcaagatctg ctgtcatttt tcctgaaaat
    76381 gctgcggata aaataagcag taagaaaaaa ccgaataacc aaaagagaag aaacggtctt
    76441 tttttcctca tgctttcctc cactcctatt taattttgac tattctatta tatacaaaaa
    76501 atgaaataga aagacaaaaa agttgaaaaa gatcgtaaat aattgaactt ctttttcaca
    76561 aaatggtaag aatggagaat ttcacgcatt tgctctcaac tattctttct taagcctcaa
    76621 atccgttttt atctgatacg tctttgaacc aatgacccga ttttttgatt gttctttttc
    76681 catcttgtgc taagtctact gaaataaagc catatcgatt cttatatgca ttggtccacg
    76741 accagttatc catacatgtc cacatatgat agcctaaaca attggcacct tcttgaatgg
    76801 cttgatggac atatttcaga tgagattgga caaattcgat ccgataatca tcgtggatca
    76861 tgccatcttc tttgataaat cgtgcttcgt tttctacacc cataccgttt tcagaaataa
    76921 agcagtcgat atttccataa tgatctcgta cattgatcaa aatatcatag attccttgtt
    76981 catagatctc ccatccgcga taaggattca ttttacgacc aggcatttca taattgtcga
    77041 aataatcctc aggcattgga ccgtttttag tatcgattgg tgtttctttt gcttttaccc
    77101 ggcgtggctg gtaataattg atacctaaaa gatctactgt attttctttg ataaccgtca
    77161 aatcctctgc atggatcaca ggcatgagat caagttcttt tactatctca atcaattcac
    77221 ttggaaactc gccttttaca gctggatcaa ggaatgaacg attgaagaac gcatctgcta
    77281 tttgcgctgc tttaacatct tcagggtttg attcgtctct tggatagctt ggtgtcagat
    77341 tcaagatgat accaataaca ccaccttgct ccatttcatg gtaagatttg atggctaacg
    77401 ctgaagccaa cgcttcatga tagccaactt ggatcgcttt tttcatatct gactcttctg
    77461 gataatgcca tcgatataaa taacctgctt cgactggaac gattggttca ttatgagtaa
    77521 accatttttt tactttcgaa ccaaacaatt caaaacaact tttggcatat ttttcatagg
    77581 cttcaaccgt ttctcggttc aaccatccgc ctttttcttg cagatacatc ggcatatcga
    77641 aatgatacag attcacaaag ggttcgatac cgttttcaat caactcatca aaataacgat
    77701 gatagaaacg aacggcttct tcgttgattg ttccagattc atcaggtatc aaacggctcc
    77761 attggataga tgttcgaaat gaattatgcc cagtctgttt catcaactgt acatcctctt
    77821 gatacttcgt ataaacttgc gaggtttttt caggtcctac ttgatcaaag aacttttgcg
    77881 gttctttttt gtaccaataa tcaaaaatgt tctctccttt tccatcgcct gaaatccttc
    77941 cttctgtttg tgggccactt gctgcagaac cccaccaaaa atttgctgga aattgatatt
    78001 ttttcatcaa agcacctctt tttctttttt agttcgtttt agttgattcc atgtatgata
    78061 ttttccatca caatcacttt ttcattttct tcctcttctt ttttgattcg ttttaacagc
    78121 tgttctgctg ctcgttcccc gattttatct gttggtggcg tgatcgtgat tttcggaagg
    78181 atactcagat cgtttttatc taaataatca tagtgactga acgcaatatc cttcccgatt
    78241 ctgacacctt cttgattgaa acattcgatg gcaccaatcg acatattgta attgctgaca
    78301 aaaatagcag ttgccccttt ttctaataat gcttttgccc cgtaatagcc gctttttcta
    78361 gaatagtctc ctgtaaagac ctctaattgg atcgatggat cattttggac cgcttctttg
    78421 gctcctgcta aacgttctct tgctacgtag tctgtttgag tggcagcaat aaatccgatt
    78481 tttttatgtc cttgttcacc atgatgagcg atcacttttg ctacactttc tcgatcattc
    78541 actaatattg tatcaatacc tgctggtcct tctggacaat tcaatgcgat aactggaata
    78601 ctcatttttg tgagcatttc aaatccagac cactcttctt cggataagaa gacaatcagc
    78661 ccatcgatca catgcttcat caggtattca attttttgtt caattttttc tggctgatcg
    78721 gcaaacccgc ttaataataa gctataccca ttttcttcag ctactctttc gatccctgag
    78781 acaacttccg acccaaaacg gctggaaatc ccgtttatca aaagtccaat agagaagctt
    78841 cgattattct tcaacccttt agcaatgatg ttttctttat agtccaacgc tgaaatcgct
    78901 tctgctattt ttttcatgtt cttttccttc agttgctgac cattcaagta acgggaaact
    78961 gttcccactg aaacaccagc catcttagcc acatcacgga tcgatgccat acattctcac
    79021 tcctgccagt tacatttgat ttctctttct tgttgttcga ttatgatgac cgcattcttt
    79081 gcatagtgtt gcttgtcatc aatatccata taactaaagt cttcagtaga gcaacttcct
    79141 ctcactatta attatactta aacaagtatt tgaaccggtt catttttaat atagcatttt
    79201 tttaaaagat tgcaacactt ttttaaaacg ctttctgaaa gtggcggatt ttttgccttt
    79261 ttaaaatttt attctaaaat ttactctatt tgtgggtgtt ttgtgcgttt ttctcttcca
    79321 tccgtttgaa gttttacatt ccaatggtat aatttcttta tcctatcaaa tgggggaagt
    79381 attttgaaga aattaatcgt actatttatt ttgtctgcgg gacttttgac attgactggc
    79441 tgtgatttgt ttggcggctc tgaccaatcc tcggacgaaa caaccgattc ttctgtctta
    79501 aaagcggaaa aagcagatac caccaacagt gtatatacgc taaaagtacg taaagatatg
    79561 gatttatccg cgggacagtt gacatttaca gataaaaaaa tggaatggac acgcacgtat
    79621 cagacacaag gaactgattc ttctgaatca tctgaagaaa aaacagtgct gacagatatc
    79681 aagatcacta ccaaagatga cgaatatatt atttcaggaa aagaaaataa aaaagaaaca
    79741 acagtcatat ttacaaaaat cggtaattac cgtttgaagg atgaggatgg caacatctac
    79801 agtctatagt gccgtctgtt ggatcttctt ttgtttctcc ccttcttttc tgctatagta
    79861 aagaagcaag atgattgtaa aggagacgaa atggatgatc gatagtcctg attattgtac
    79921 atgtaataac aacaaaaata tccatacaga atttaatgac caaggaagct tgagcatatg
    79981 cgacaactgt cacaaactag tcaaagattc atttaaaaca tttgaatctt atcctgttca
    80041 aaattatgta aaaagtagaa gaagataaac aaatccaagc aaaactcact atggctgatt
    80101 tttatgaact tcaaactcag cttcttcctt tgatcaagct tcatcagtaa aacaaacagt
    80161 ggatcatttc agtttacaaa tcaaagagta aagatttatt tatttcaaat atttgaatat
    80221 aaaataacca aacgatagaa aggcttattc tacatcacgt agtaattttt ctttcccgtc
    80281 gtttggtttt ttatttttag cctaattctt gaccttttgc atgctggtta tccgtaaaat
    80341 attttccagc aggctttcct tccaattcag ctaatttttc ttggatctga cggatcacct
    80401 tttttgcatc ttctggattt tctacaaaat cgaatttatc cccatccact tgtattttag
    80461 gacatacatc aaaatcttca taccattgat tatagcgggt gttcaacgtt tcataatagg
    80521 tatataacgt ctcatcgctt tctagctgtt catagtcacg cccacgtttt ttgatacgtt
    80581 ctagcatcgt gtccaaagaa actttgatat ggaccatcaa atcaggccgt ttttttggag
    80641 caacatcatc taactcgttt agcatcgtat ctaataagct atcgtactgc tgtacttcga
    80701 tatccgttac ccgacctaaa tcagcgttta gatggaacaa caggctatct tcatagatgg
    80761 atcggtctag cacattgtca tcgttcacta aagcattttt cattgctaaa aatcgcttgt
    80821 tcaaaaagaa gatttgcaat aaaaaagcgt actgctcagg atttgaataa aagagcggca
    80881 atacttcgtt atcttcgaca ttttcataaa atggtttaga atcaatctct tttgcaagca
    80941 tatccgtcaa actgctctta cctgcaccaa tcgttcctga aatcaaaatc actgacatac
    81001 aacccttcct cttctataag tactcgccta cgtaagtttc aacaggcaat gtttcaaaat
    81061 catattcacg tacagcttgg tctgatttta aatacgtatg gagactttgt gtaatcatca
    81121 agttcgtagg taccgtctct tcttgtacaa tgatcatcgg ttttttcaac atagttgcca
    81181 cacctaattc gtaagccgtt cctggatcga ttgtttgatg ctcaaagtca aggatagcaa
    81241 caacaaattc tgcatttgtc agttcttcca tatctttttc gtatacttcc tgcgcccatc
    81301 cagcgctgaa tagttcataa ttgctttctt ggtgatgacg tggagagtag aaacttgtga
    81361 ctgttttgtt ttcctccaac gctttttcga tacgacttac tcgatcgatt tgctcctctg
    81421 aaaaaaatgg tcctgctaaa tatatttgac tcattatttt gcctccttca ataataactg
    81481 atttttaata gatactgctc gtaattcatt gaccagttcc aatggattgt tggaaaagat
    81541 tacttgatcc aaccattttt tatccataaa atcctctttt gcaaaagttt ctaataattg
    81601 ttgcaagccg ctgtaataac cgccaatatt caatattccg attggcttgt cgggcgtcag
    81661 accgattgct atccaagaaa gaagttgtga gaattcctcc aatgtaccta gacctccagg
    81721 gaaaacgagg acagcatctc ccttttcaaa taataatacc tttctttcat cgatcgtttc
    81781 tgtaggaata aatgtagtaa catcttttcc aggcaattca gtttcaaaaa gcccattcgg
    81841 atagatacct gtgacttttc cgttattttc cagtactgtt ttactaatca ctcccataat
    81901 cccggagtat gaaccaccgt aaaccagttc gatttcttct tgtgccatgt attttcctaa
    81961 cgtttgcgca ataaatttgt aactttcttt attaccaaaa cgggaaccgc aaaaaacagt
    82021 aagtttcata aaaaaacact ccctaactca aaagtctatc ttcattctac acgtgttttt
    82081 ttaaaataaa agactagatt ttgtagtttt ttagtggtat ataggataga cgccactata
    82141 tatagtattt agaaaaaaat catttttcag ccattttata gataaaacaa aaataaataa
    82201 cgtgttttca tcgatatgaa atgacttact tatagaagat actgggattt cgtcctatat
    82261 gccaataacg tgtattattt taaacaacaa atacttgttt cttttattaa cgaaaaaatc
    82321 attttgttct cgtgttatgt ttcactgata aatgatgttt ctctaaataa aaaagccgac
    82381 gaactaaacg atcaaaattt gatcattttt gttcatcagc ttctcctaaa aatctttttg
    82441 ttaacgataa atagctggat ttgtaaaaat atcacttgaa tcgtcactgc tggaagaaaa
    82501 ttcagtgata accgcacctg ttgcgcctgc ctggaactag tgtttgatat tgggctcgaa
    82561 cgtgtactga tctctagcat gaagaataat ttcttttttt actgtataat aactttcttt
    82621 cctttttgga atagtacagg aaagtttttt tgtttccttt ccttcgacat aaggtatacc
    82681 agaccctttc gacaacgaaa ggttctcatt tttccttcat cgtcatttca aggagggtgc
    82741 cgatgctctg gacagtttcg atcgctccta aaccaaaatc tgtatattcg attctttcta
    82801 actctgattc tttcatacca ttcctgtctc aaggtacatc tattgtactt ttttcttgta
    82861 atccattctg cctctcctaa ctaattgtca aatcatccca agatatgtct tcttcctctt
    82921 tttcttcttg gatgatttct gcattcggat ctactggttt tttcaataat aaaatggtta
    82981 ttcctgtgac aaaagaacca gctaacaatg caccaatgaa tgcccatggt ctggtcatcg
    83041 tagcgactgc aaacattccc gatgccggaa tacggctatc tgcgccccaa aacatgctta
    83101 atccgccacc tactgtccca ccgactcccg ttgctatgac attgcgtgcc aagtcgttca
    83161 atacgatagg gaaacatccc tctgtgatct gacagatccc cataggaaaa gcggtcttca
    83221 atgtatcgat ttcttgacga ttgagtatat ttttacgaat gattttccct aagaaataag
    83281 cgatcgtcaa gccgaatggg gttaccatcg aagctaaaat caatacagtg attggttcat
    83341 agattccttc tgaaaatcgt gccacacccc cttatccaga ataaatggta atccagaaac
    83401 agccgtgtat ctgcggtaat aagcctaacc aaacgaaaaa tatgagaagc tatttttgtt
    83461 tggctgttct tattacttga tgcagggcag ctttttcaca acctcttttt ttatttatgt
    83521 ttcataatca atatggtaag gatgcagctc cgtatggtag accgcctccg aatattcaat
    83581 catttcccca atttgcgtag acgatttccg tatccgtttg atgccgatct gtgcgtctgt
    83641 tttcagaatt ccttgttctt cttctgttaa aaatactgcc gagaaatcat cagaaaatga
    83701 gtcaatagta tatccttttt tcgtcagcaa acgatacaaa gattctttgc caaattcttc
    83761 tctgctgaat tgcatcattt cttgtggaag ataatgaaca aaataaatat aaggctgatt
    83821 gttcaaataa tataagcgct taaaacaaac agcttgctca ccgaaaaagg aaaacaaagg
    83881 atcttccttc tttatatgga ccaattgcaa atccagcgtt tctttccgga cttcatatcg
    83941 ggagttctcc aagatttgcg taaagcttgc cgctttggat aatcgattgt aaggacgatt
    84001 gcttagaacc gtcgtcccac gaccactttt ctttgcgaca tattcatctg atgccagcag
    84061 ttcgattgcc tttcggacag tgattttgct gacttgaaac agttcttcta attcactttc
    84121 tgtcggcaac atcgaaccga ctggatattt tcctgagaaa atatcttctt taattttatc
    84181 tgcgacttcc aagtatagta cacgtgtcct tttcataacg tcaccctttg ctttactatt
    84241 actaaaagaa tagcacataa aaaataaaaa aagaacacaa atcacatgtg agaagtgtgt
    84301 tctttttcat tcagctgctt taattacaag tttacgccat ttgaggcaat tacttcttta
    84361 taccaatcaa aagatttttt ctttgatcga ttcagtgtcc catttccttc atcgtctaga
    84421 tccacataga taaagccgta acgtttactc atttctcctg ttgatgcact gacaagatcg
    84481 atacatcccc aaggcgtata gcccatcaaa tctacaccgt ctgctactgc attagccatc
    84541 gcttcgatat gacgacgtaa ataatcgatc cgataatcat caataatcgt gccatcctct
    84601 tccatatgat caatggctcc aagaccgttt tcaacaatga ataatggttt ctggtaacga
    84661 ttgtataatt cattcaatgc gattctcagc ccttctggat cgatctgcca gccccattca
    84721 cttgcctcaa gaaacggatt ttttactcct cccagcaagt tcccttctga agttgctgct
    84781 tgtggatcag tctcattgat tgccgtagac atataataac taaatccgat gtagtccact
    84841 ggatattctt ttagcaactg gaagtctttt tccgtttgct ctaatttttc cggatcgact
    84901 ccatattttt tatgggtacg cgctgtgtat gcagggtatt ctccacgtac ttggacatcc
    84961 gtgcagaaaa aattgaattc ttggttttgg atcattgttg ctgcctgatt gactggattg
    85021 gcatcgatgc tgtaagttgt tgcatagata atcatgcagc cgatttggat atccgaacgc
    85081 aattcgtggc cgatttttac ggctttgcta cttgcaacga attgattatg ccatgcttgg
    85141 aaaatatttt ggtactctcc tgctccattg ctttttacta atccttgact taaagctgga
    85201 aagtgaaagg ccgaattgat ttcattgaaa gtcatccaat atttgacttt actgctgtat
    85261 ctctctaata ctgtctgagc aaatcgttca tagaagtcga tcagttttcg atttttccag
    85321 ccaccgtatt cttttgccaa atgaagaggc atttcgtaat gagaaatcgt gatcacaggt
    85381 tcaatatcat attttaagca ctcatcaatc agctgatcat aaaattctaa tcccgcttcg
    85441 ttaggagtac cttcgtctcc atttggaaaa attcgtgtcc aggcaatcga aaaacgataa
    85501 catttgaatc ccatttccgc aaacaaggca agatcttctt tgaaacgatc gtaatgatcg
    85561 attcctcgat gattcgggta aatatatttt tcttgatcaa tcgtccagtc aaattcttca
    85621 ctgccgataa tcgcaaagcg ctgcttacct ccaggcatag catctgcaac ggatagtcct
    85681 tttccgtctc gatcataagc cccttctaat tgatttggca gcagttgctc caccccataa
    85741 aaatttttct gaaaaacgtg attgcatcct gatttcctcc ttattttaca attgccagaa
    85801 catcttcgtt tgcaataatc tctttttggt ctaattcaag tatatcggca aatgcatcag
    85861 tattcgtaat gacaactgga gtgattacgc tatagccagc tgctctaatt gcttcgatgt
    85921 caaaacgaat caataaatct cctgctttga cgagatcccc ttgtttgact aagatctcaa
    85981 agccattccc atttaattct actgtatcca tcccgatatg aatcaaaatc tccgctccgt
    86041 cagtcgttgt gatccctaca gcatgtccag ttggaaaaat agtcgtgatc tctccgtctg
    86101 caggtgcaac tagttctcct acttctggat caatagcgat accttttccc atcacgccag
    86161 aagaaaaaac ttggtctggt acttcactca atggcagtac tttacccgtt aaaggacttt
    86221 ttaaggtatg ccgtgctgaa gtaatcggtt ttcctgcatt cgcatgtttg ttttctaatt
    86281 gattgtcttg tgtttgttcg tcaaatccta aaatcaatgt acctacgaat gcaagaacaa
    86341 aagcaattcc tgtagctatt actgctactg tcacatttga ctctacacca tccaccgtat
    86401 taatgaaagt cggaatcgtc aataagctga ccaaactgct ggagaaagct tttactccgg
    86461 aaaatccgat aatagctcca ccaatacctc cagaaataca agcggcaatg aatggtttct
    86521 tcaaaggtaa tgtcacacca tacacagttg gttcagtgat tccaaataac gaagtcactg
    86581 tagaagaaat ccctaatgct cgaagtttcg tatcttttgt tcgcaatgct acagctaaag
    86641 cagccccacc ttgtgccaag atggctggaa gaagcattgg catcaataca tcaaacccat
    86701 attgttcgat attcaaaaac atgatcggta cgaatcccca atgcatgccg aacatcacga
    86761 atacttgcca taaacttccc atgattagac ctgctacgat tggactaaat ccataaatcg
    86821 agttgaagag accgcctaaa gcattaccgg caaccgtccc gattggtcct aaagcaagaa
    86881 atgttaatgg aaccatcact actaaaacga caagcggaac taaaatcatt tgcagaaatt
    86941 gcggaatcac tttttttacg aatggctcta atttactttg aacccatacg gctagaatga
    87001 ttggtatgac agaagaagta taaccagatg gtgaaagaat cactgggatc ccaaagaaac
    87061 tgatagcttc tcccgcacca gcaactgctg tgatagctgg atacacaagt gccatagcaa
    87121 ttgctactgc taaaaactgg ttggctttga actttttagc tgctgtaaaa gcaagcatga
    87181 caggtaagaa ggtaaagaca ccatctgctg cagcaaacaa tatttgataa gtaccgctat
    87241 cagctgtcag ccagcccatc accgttgcca atgataagaa tcctttcaat accccggcgg
    87301 ctgccatcgc ccctagaaaa ggtgtaaata tactagagat aatatcgatc agacgattca
    87361 aaatattttt ggactcttct tttccatcct ccgaaactgc aagaccttcc tcatccacga
    87421 tcgattgata aacatctgct acattactgc caatcactac ttgatactga ccgccacttt
    87481 gtacgacttg gatcacgtcc gggtcttctt ttaacttttg cgtatctgct aaagattcat
    87541 cttttaatct gaatcgcaac cgcgtagcac aatgtactaa acttttgaca ttcttttgtc
    87601 ctcctaccgc ttcccataca cgacgaccaa ctgcttgatt atccattgtt ttttccttct
    87661 ttctttttta tttaaaaaat aaaaacccag attgaaaaaa acagtcacaa acattcgtga
    87721 acctattttt tcaatccagg ttttgcctgc catacagtaa caatccttat gattggatgc
    87781 tttttttctc acttactact ctttgaatat gaatcgttaa ataaatctgc tctgcgatcc
    87841 ccatttcctg tttttgcgtt tgtcgaagat actcattgat ccgttcgaca caatgaaacg
    87901 cttttggata ctttccttga actaatgcgt agagaaattc atcttgctcg tcagatcctt
    87961 cccctttgag ataacgctgg gcaaaaaact gcagatgagt aatgatacgc tgataattca
    88021 aagattcttc atcgaacacg atgccgaaga atttactgat gatactcagt atatctctta
    88081 ccattgtggt agcggtcatc gtcacttgca tgtcttcgtt tccttgttgg ctattcacaa
    88141 aatgaaaggc aataaaaccg gcttcatcct cagaaagttc cacacccgtc ttttgtttca
    88201 cgaacaaaag agcctctttc gctgcttgat attctttcgg ataaaatttt tttgtttcaa
    88261 ataataatgg attaggcagt tggatatttt cttttgctcg tgtgatcgca taatgaagat
    88321 gatcggtcaa tgtcagataa atattaggcg aaaattcaac ttttagttta tcttcagcca
    88381 gttcgactac ttgtttgacc cattcgatct cctctgccgg aatatcagcc atcagctgct
    88441 ggaattggtt tttagcaaaa gaatctctta aaacaaattc tttttggacc gcttctggcg
    88501 aaatctcgtc cccaatcttt ttttgaaaag ctagtccttt tcccatacaa acgacttctt
    88561 ccccgaatcc atttttcgaa atgacgacat tgttattcaa aattttatca attttcattt
    88621 tgcttcgccc tctcaagaaa aataaaaaaa cctagaattc gcctactgtt tcttagctga
    88681 attctggttt tgcctgccgt acagtaacaa tccatttctt attcaaaatc atcataccat
    88741 cgaattttta tttatgcaag ccttttcata taaaaaataa aaaaactgga acacaagtct
    88801 atctagaaaa caaccaaatc agagatgcac ctctttgcgt ttctcttttt ttcagcaaat
    88861 tggtttttcc ttatcgattc atatcctttg gctgttttta atctgtaacg acttatgtcc
    88921 cagtatccta aaatgttttt gtctctagat tgaaagcaga tctcatatca tctgtcgcat
    88981 cttttatcga tgtaacactt tccagttctc cttgtacaac tagtcgcgtg acggcgtcca
    89041 tgtcgccaca agtaattagt gtgacaattt cttttccctc gacttcattc agtaattggg
    89101 tatctgtagg agcaactttt tctttaaaaa atgttttgta tgtatacaca ttttctaaat
    89161 ctgtcaaata gatcttggca cccatctcta aattttctag cggtgtaaac agcaattcag
    89221 ggttcgttgc acgatggctt gctaaggcat aatttccttt tcccatttct tgatctggag
    89281 ataatgttcc tgctccgtaa agtagtgacg tattatccaa gcctttgaaa accggtaaat
    89341 tgattgaaac tgaagggact gcgatactag caatcactgg tagatctgtt cctctcattt
    89401 gcgacctcat gaccccttct gtcgtcattg gttctaccgc atcaaagtca aaactgacat
    89461 ctttatcgtt attctttttc agatcttctt ttgttacatt cgctatagca tatttatctc
    89521 ctgtctctct gacaaaataa tccttgatct gttcattgaa aatcaaagct agtcctacta
    89581 ataacaagag gaacaagagg atattgacta gccatctttt tgcttttcct ttttttttca
    89641 ttttctctcc ctccagactc attttactgt ttttcttttg ctctttccaa cagccgaatg
    89701 acacatttct acaactctgt gtattttttt cgtacacctg cgcttttttc tacaggatat
    89761 ttcaaattat ttttgatcaa tttttcttca atgatctcat ctagatccag gtgaaggttg
    89821 tctgccatca tgtatgaata gatcaataca tctgccagtt cttctttgat tcgttctaat
    89881 tgtgtttctt ttacttcttc cggcgttttc cattgaaaaa gttctaacaa ttcagatgct
    89941 tccaaagaaa tagagatagc caaatctttt tcgttatgaa attgtcgcca attacgttca
    90001 tctctaaact ggttgacttt ttccatacta ttcatttgct tgccttcttc caatcacttc
    90061 ttacttgatt aatcatacca tttttttaca taaaaaatag atgatgcttt taaaaaaact
    90121 cctgttttct tttatttaaa ataaatgtca tagaaaatca ataatttagg tagccaccat
    90181 gtgtatgcag tagcgtgtct tcttcccttg aggctttttg tttttcgata cgttcttctt
    90241 cccaaacaga caacgctact agaatctctc ttaatgattt tccttcttcg gtgagcgagt
    90301 actctacttt aggcgggact tggttatagg cttttcgatg gatactttca tcttgttcta
    90361 actctcttaa ctgctgggtc agcatttttt ttgtgatcgt agggatttgc ttgcttctcc
    90421 acgcctaaaa taagcgatat tgctcttccc tttatctggt gtaagcaaag cagttagcat
    90481 tttgacgatc gtgggttccg cttttcactt cttatctact tagtatcatg aagtatactt
    90541 tcgtactaat actaagtagc ggaagtgtca actactttgt ttaactacat agcaaaagaa
    90601 acagaagggt gacaacggtc atcacgcttc tgtttctttt gaatatgctg tgttgttgtc
    90661 tctatctccg tgtagacttt cctcaaactt atcctttgac agtaagtcag taggctgatt
    90721 tgctctttct agatgccaga tacatttcta caaaaaaata gtgattctgt tttgaaaatt
    90781 cgatttttag ttctgcattt tcgtaaacat ccaacatttt tttcgcactg tatagaccta
    90841 acccgccatt ttttttagag gtttttcctc ttttgaccat ctccgatagc gtcaaattcg
    90901 ttggatttgc cttgttcgct atactaaaac gtgatccctc tttttcttcc agcagattga
    90961 tcgtaatcgg actctgatcg gctacacgat gatcgaatgc gttagaaata aaaatagata
    91021 aacagcgaat aaaaacaacc agatcaatcg ggattgtttt cacttcgaca ggaataacta
    91081 cttggatcgg gattttctcc gattctgctt tttctagaaa ttttaaaaag acacccctga
    91141 cagctgggga tacaatattt gttagctgtt gtatttgcgg tttttcaaaa atagctgcag
    91201 aatgatcttc taatatttct aaaaattcca acgtttcttt tttattttcc tgaagagtaa
    91261 tttttaatga aaacagtaga cttttataat catgtttgaa ttcctctaga tgatggtaat
    91321 attcttcatt tatttgatac agttgaagcg attcctgatg ttgtctttct tttacttgga
    91381 aactgatcaa cataatggaa aaaaacaaag aaaaaaaact gatgactatc gacaaaagag
    91441 atagaagcag ataattagat gagcggaagt gaacacttcc caataccaag taaatcacgc
    91501 cccagcttaa taaaatacaa atacctccaa aatagtgttc agttggataa gtaaaaagca
    91561 agttgagaaa tctcgtctgt ttgtctatac tttttactaa tagatagaaa ataaatgcga
    91621 taagtagtgc aaaaaaactt gctgttccaa gatgtctctt tcctaaataa tccatcagat
    91681 caaaaaccag aaaaaagctg agtccagtga tagcaaaaaa aagattactg atcaaacagc
    91741 tgataaacag gtttttcgta gtaaaataaa aaagaaacat caataataag ccaactaggt
    91801 aaggaaagct cactttaggg ataggaagta aaagtgtcac caccatcaaa gctatacaac
    91861 caaagctgtt ttttactagc agttgtttcg aaaagaataa atagatacct aaatctaaaa
    91921 tcaaagtaaa ggtcaaataa agtgtcactg ttgcgaccat cttattcctc cattgttttg
    91981 tttcaaaatg tacacatatt tttttaatag gagcctgcat atgaaaaagt attttatcat
    92041 agaagataac cagtttcaca gcgaatggat catcagagaa ttggtcaatc atcatatcga
    92101 gaatatcgtg cagctcactt ctatctctca aatcaatcag ttgatccagt tgggcactat
    92161 tgataatcac gatgttttct ttatcgatat cgacttgaag caatcactca caggtattga
    92221 tgtcgccgaa caaatacgta aaaaaaatgc acatagtaat attgtttttt tcaccgctta
    92281 tacgaatttt gcagtagaga tcatcaatcg gaaaatcctt ccgattggat atatcagaaa
    92341 agacaggaat ttatctgaac aaattcgtca agtgctagaa cagatcaatt ggaaagaaca
    92401 gcagatcgta aatgtcgata aaatctttat tgatcaaaaa ggtggaagtc tcgtcctgta
    92461 tcctcgggaa attttatata ttgaaagtat caaaggtttg aaaaaccaag ttatggtcaa
    92521 aacaactaca gaagataaat tagtgaatac ttctttaaaa caattgaaga cagagttaga
    92581 aaagtatgct tactttttac ttttaaaatc ttactgtatc aacttatcgc aattgcagaa
    92641 aattgattct gctcattatg tgttagaatt tttcaacggt gactccttac tcgttggtcg
    92701 aaagatcttt gaaaagacaa aggaacgttt ccatgacttc caaaaaactg cttcgagttg
    92761 atgaataaga gctgagacgt aagcatagca gatttcaggg agtgggcctt gaatcggctc
    92821 cactccctga aatcgttgat atgattttac gcttcctctt ctgtaggagc atctgttaac
    92881 gaccatgtga atgttgaaac gtagttgtct acagcataat tggatacagg aacaaataat
    92941 tccactgatt gtctcccttg ttccacattc tctcctaatg aataaatcca tgtgccaatc
    93001 ccttcatttg ctcctgcagt tactagatct actgcctgat ctgctgggat ttcgattcct
    93061 tcagcgccag ttccagaaac ggtagatggc attccactgc tattagttgc gctatttcga
    93121 atcacggcgt ttttgaattt cacctttgaa ccagttagtt tattcgtatc agatgttgta
    93181 ccaataaatt ctgttcgttt gactgataat ttccaaccgg cataatttcc gcttttatcc
    93241 gttacttgta cgaaattctg tacttgtgtt tgtgctccag tactgtccgt tccgctttgt
    93301 aatgcagcat atactgtttt tgctgtacct aacttgattg ttccaaaatc aagatctgga
    93361 gcttggtcaa tcgtcagtaa tccggatgtt ccctgatttg gttcgtcagg tgtaaaagga
    93421 gtatcaggat catttggatc aacaggagga actggaacaa cgtcttcagg atttgcagca
    93481 tctggtttgg tcacagaaat cgttccttct gtagtggctg agctcttatt ctcagcaaaa
    93541 acactttgtg tacataataa tagacttcca gctagaacgg tacttaatgc aattgttttt
    93601 ttcatttctt tttccttctt tcttttaaaa taaataatcc agcaaataag ccaataagaa
    93661 tgactccaac aagtatcagc catcgccagt caaatgtttc tggctgattt tttaaatcaa
    93721 tatccttttc attcaattgg tccgctgcat catcagttat ttcaaaatcg gtatggagat
    93781 tccagcgttt ttctttccaa gcacccttca caaccgcttg ataatgtccc ggagctaatt
    93841 tttttccttc taatgggaca agtaaatcaa aatttgtatt gggtgctaca tctatttttt
    93901 ctttttttat tgaaaggtaa ggctcttttg tttctttttt gtagatttca accgatgtag
    93961 tcaagccgaa catataagaa gcatttggat tttgaagatt cgccttaatc acatttcttc
    94021 catcgctttg atccgcataa gcttttttta atttcaaatc tggggaagac tctttttcgc
    94081 tatttcaaat caagattgct ttcacttgag aaaactgatt gacgacacca actgtacttt
    94141 tgttagaaac gttctctttt ttttcttgaa acgtgatccc tcctgcgacc acaccagcca
    94201 gctctttttc tggcgctttc agtgaaagtg tctcagtgac cgcactttga ggaggaacag
    94261 ttatttctcc cactgttttt gctaagtttt tcatatcata acttaatgtt tcatcaaatt
    94321 tactttttga ttctccatat tcgatcacac cgtttgaatt cgtcgtagcg gagtggatca
    94381 atgtctcaac tgttactgat ttatctgttc ggttaaagac cgtgtagggg atttcctgtg
    94441 ttttcccagg ctccatcctt acatttatat atgttttgtt cttatcaatc tgattagccg
    94501 gtaattcaaa atctaccgca aaattgaatt cggaggcatg gacaggtgtc tgccaaagga
    94561 tgaatccaag gagaaggatc ataagtaaag agcagttgtt tcgatgactt ttattccata
    94621 ttcgattcat caatcatcct ccttgatctt tattctgtcg gcgcatctgc taatgtccat
    94681 gttatctctg ctgagtaatt accagctatc tgattttctt tggttaagac aagtttaaac
    94741 gcccgaccgt tatttaaata ttctgaataa tctgcttgca cattcgttga attttgtcct
    94801 ctgacaattt ccagttcttc ttcattcact aattgatact cctctccttt cttgaaatat
    94861 aggacatcct gcatagaaaa tcctgcatca tttgtgaaag gctgagataa ttttatcgtt
    94921 aagatccacg gatttttcgt ttctcgctga tcataaatcc ataatttttc ctgtatatcc
    94981 atttggccaa atgcttcttg tgtcaaacca gttgctttga tctgaccaaa atcgagtttg
    95041 gaaggggcaa gaaatgagag accaccttct actacgatct tcgtagcagg tgcaaaaggc
    95101 ggggcatcat gataaacaac tggcgtatca cttggattgt gattcccaat ttcatcgttg
    95161 gtacttggct tgttctgcac acccttagca gtagcttctc ctgcttggtc gtttaataca
    95221 atttctaata catctcctgc aaaaagttga ttttctggga tagaaagaac aaattcccct
    95281 gtatcatcaa tagttgtttc tgacaaaaca gcttgatttt ttttcaatga aaccaaggct
    95341 cctgcttcac ctgtacctgt gatcgtgcga gaagctgcat tgatcaatgt ggtacttaaa
    95401 ttagcaggtt caggaggaat cacatcgact gtcgttttgg cagagtccag ttctgtcact
    95461 tcttggccac ctcttacttt tttagcagca acaacttgca cttgatcctc cgtttttaat
    95521 ctctcatcat tgggtacttg aacttcgaac aagccgcctt gttcgccttc tccccatggg
    95581 ttatatcctg ccccttcatt ttcatctgtt tgtccttgcg tgagagcagt aatttctaca
    95641 gatgatttcg tcgtatctgc gtaagtaata cgtaaagtca cttctacttc gcctgtccaa
    95701 gcatctcttg gctttgcctc gacaccttcg ggaacaacga catgcccata gattttttta
    95761 tctgcattcg ttggcactct taaggattca atcatcggga gctgattatt ggcactgatt
    95821 cgattataat ttttcattcc ggcacctgtc tgagcaaatt catttgttaa acctgcacta
    95881 gggcttgata cgaaattgcc taagtcggtc ccattgatcg ataagtctgt catatcagaa
    95941 aaataatccg ggtctttttc aaagttatcg aatgttgcgc ctgtttttcg ccataaagcg
    96001 atttctgaat gggcgatctt taaagaagaa tctccatttc cttggaatac ccagccgcct
    96061 atgccattgg tctcgcttcc aatccgtttg ttttggaaat caaaataggt aggagattct
    96121 aagtccacat tcaacacatc cccactgatg atgccggtct tatcttcccc tgttttcccg
    96181 accatgacta aataagtttt ttcacctgcc gtaaaattta aatcaatatc tccagttgta
    96241 tctacagttg ctccggaatc tgcgcgtata tcgatatttg atttttctcc agtgactgta
    96301 aaatcagctg aacctggact ttctagaaaa ccagaagcgt cataaaattg gatcgcttga
    96361 tttccttgat tgagtctgtt cacgtaagat ttgccatcac ctgtatttct taccctcaat
    96421 gagcctccat tggttactgt cagcttttgc gtaccattct caaaacgaac tgcgctggaa
    96481 cgcccactct gcttatcgat gtcgataact gatccggctc cttctacgat caaagaaagg
    96541 ccttttccta caaaacgcat cccattgttc ccgggttgat cgtcatctcc agccattttg
    96601 accgagattt gtccaccact tgatacatgg atttgactag aatctccttg caagcgaaat
    96661 ccgcccgtag ttgctgtatt gatggtgatt ttcccaccat tcgatacagt gactttcgat
    96721 cctgaacctc tcatttggat gattccgcga ttgttgtcat tggtaatatt gatctttgta
    96781 tttaccgaaa attcgcttcc cgaaccactg acttcaaaca tgacattacc aggtagcgta
    96841 tcgactgcct ctccttcatc gctaacgcca tctgcccgat cataatcaag agagaccttt
    96901 gaaccgctta agatatggat tcctggcgct gtttttccac taccagtctc atctcctgaa
    96961 tctagaaaac cgtagaattt ccctagatac ggtccataaa gttgactacc attgatcaca
    97021 ttcaattgat agcttctaca aaaataggct ttacccggag cggcattcag tccttccttc
    97081 actggttgga catccaacac accggaaaca ccatctaatg tgacagttgc acgaggagcg
    97141 taaatcagtc ctaatttgga cccatcatac aaatcaagtg tcccggtaaa ttttatttct
    97201 cccgtaaaat gatatctgct tccccaaaaa tcggtcatat aagcaatgat aatggcattt
    97261 ccatcgatat ttggtgtcaa tccacctgga tgtgcagctg ttcctttaaa attctggaac
    97321 tcgatcgact gaggtatctc attgatataa atatggtgtg tgccgaaatt gtgagcgaat
    97381 ccaccaaaat caataacgat atctcgtttg atctctaatt ccctgtctaa agtcaaaggc
    97441 tgaatcagtc ggatcttagt tactgattcg tctgcgagaa gctgcgccag ttcttctgtt
    97501 gtggcagctt cgctgtaccc atccgctgaa acagctcttg ccagtgccgc ttcactgcta
    97561 tcagacgttt gttcagctac ttctgaggtc tcacttgtct tattttcttc ttgcctttta
    97621 ttagtagaag cctcactttg agattctgta gtttcagaag taatcgattc ttctgaaaaa
    97681 gtagtcttgt caaaactttg attctctgat tgcgttgatt cgtttgtttt ctctacatcg
    97741 gcaaaaacgg atgtcaccat caagagaggg aagacaaaca aaatcgacgc aatcagcttt
    97801 tttacacatt gattcattct ccaccatcct ctctgacgtt ttgacaattt tattttatcg
    97861 ttttaatcta tttccaattg tactttcata tttaaaatat gtatttttat ggaaaaattt
    97921 ttctattagg tgtaaatttc ttttttgtac aaaaaaaagg ctgtgacaaa agtcttgtca
    97981 cagtccctta tcccgaacaa gcggtgttct cccgccaact cctcggtcac aacctccatt
    98041 ttaacaataa acctgtaaat gtgagggcaa tactttgatt gtcacaggta atttatcccc
    98101 ttcatctcca tcaacattcg tcgtcaattc ttcctcatca gctaatgaca caactaattg
    98161 ctcacaagta aggtaggcta gatttttcgt agattcatct actcctttta ataggtctgg
    98221 caatgtttta agcgtatcga tcaaagaaga atcttttaaa taaaccaaat ggagcttacc
    98281 atcatccact tttgcatttg gaagtaatgt ttcaaaaccg ccgactgagt tagttagtcc
    98341 tatcagcaag gtactgctct caatttcttc cttctttcca tcaacttcta cgtgaaacga
    98401 gtaggattgt gtggaagcta actgtttgat tcctgacatg aaataagcta gttttccaaa
    98461 tttcgtcttc ttctccgcat ctacatcatt gattgattca gggatagagc caattgcgac
    98521 gacattcata aaatagtcgt catttatttt tccaatatcc aatggcttga ctgattcaat
    98581 cgaaaaatga ttgatcgctt cctcaggttc tagcggtatc cctaatgctc gagccaagtc
    98641 attcaccgta cccaatggga aaaatccgaa atttggccga tgttcttgtt cagcgattcc
    98701 actgattcct tcgttgactg ttccatctcc gcccatgaca aacacactat gatacccttc
    98761 cgtagccgct tcgcgagtaa agttttttgc atcccctgct ttttttgtat gcaagacgac
    98821 cacttcatca aacaccgatt ccaattttgc tatcgctaat tgctcaaatt cctttgcttg
    98881 ttcaccgcct gaactggggt ttaccactaa taaaactttt ttcatcactt tctttccctt
    98941 ctgattgatt tctttaatat aacgatagca taaagaaaaa aattaggctg tgatgaagta
    99001 ctgttcatga aacattcatt tattttttga caagataaaa catccatact atgaaactga
    99061 ggaaaattaa aacaatgata taggaaataa aagaaaatga taggcattgt atacctaata
    99121 aagtaaaaat cttaggtggc agataaaaac acaaaaatag caagagaatg taatagcgtt
    99181 tcttccactt tttgttatca gaccttatag cgatcagagg aataccaaat gcataactga
    99241 aaaactcttg atgtactgtt aatatgtcac ggccttgaat aataaatgcg acaatccccg
    99301 ctataattag tgtgcctaac accaaaacgg aaaattgatg aatatatttt atatacaaat
    99361 gatccattgt acttcctgcc tttttatttg tgagaaaaac aaatccgtat acgtgaataa
    99421 cttctagaaa taaaagaaat ttgcaaaacc actccattta tctacttcta cgtgcatttt
    99481 ttgatattgt tttctttatt taagattata cagaaaaata tctagggtcg ctggattcta
    99541 agtaaaaaaa atttttaaaa taaaaagaac taaatcaagt agtaattaaa ctactgttga
    99601 tttagttcta ataaaaagat ttagatgaac agttttattg atttaaatag ttcatatttt
    99661 ttatttatac cgcttattta ctacttgctg tactagaatc tgaagtgaat gtactcaaaa
    99721 cattttcaaa gtctttgtct ttgattttta cattagcgtc tttcaatact tcgccaatta
    99781 ctttattctg gaaggtactg tcacttaatt ttgtgtctgt tgcaatttct ttcaattctt
    99841 ttttgtattt atccatgtcg ttgcctttgt tttggttttt caccatttta accacataat
    99901 attccgtagt ataagttgaa gcattggtag aagtgatcac atcagaaact tgaccatctt
    99961 tcaacttaaa ggctgcttct ttgacttctg ccggaaccgt tgtagtagtg gaatcaaatt
   100021 tcacttttcc gccatcttct tttgtcgtag tatcagttga cttgtctttg gctaatttag
   100081 agaaatcgtc tcccttatca gcagctttct tcacatcttt tgcatcatct tcacttgcta
   100141 cttggataat ttgagcttcc acttctggat ggaaagattc ccaagccgtt tttaaatctt
   100201 catcggtcaa tttgatatgt ttctttagac cttcttgaaa ggctagactt tgtttgatac
   100261 tgtctttgaa tgtttgttct gtgtaacctg cagatttcaa ttgtgaatca aatgaatcac
   100321 ccaattgttt ctttgtttga tcatattgag aatcgatttc tttggttgat acgtcatctc
   100381 catatttatc ttcaaatact tgataaacga tcaactgttg cacagcggaa tctgctcctt
   100441 gcattttttt caagctatcc attacttctt gttcgctgat tgtaccgcct ttataactaa
   100501 cggctgtttt ttcaccggca ttcgtacagc ctgacaataa gccagtgcct agtacgatgg
   100561 caagtgctaa aaatcttttt ttcatttatc aattctccta cttttcgttt tttgcgaatt
   100621 gctaaaaaca aaggtgttca agatgaaaat catcaagaac acctttacta atctgctcga
   100681 tacgtcctga atagatataa ggctacacaa tccagaaagt tcgctcagca gaataaatgt
   100741 caagtagcac atcacacctt aatatactac ttcttttgtt accgtttttc aagtctatct
   100801 tgcgttccgt atttaacgta actaattctt agaacaaatg ttatcgattt tgtttttctt
   100861 cttccgctgc tgggatattc aaatcagaca cttttatgtt cgattttcca gacatttttt
   100921 tgttgttttt attatgatat atttttatac aactaaaaat gaatatatta aaactaccaa
   100981 aaataacttt taacgctgtt tcattatatg ttttttctat tttatataaa cttttggata
   101041 cttaatacca accggttgga ttgtaccaac atgagaatgt gtcagttcta tttcctgtcg
   101101 cacttgcata ggcccatact ccttttgttg catttgctga tcgaatagag tttccaactt
   101161 tcgcttttgc accgtgacga ttatagacac gactatagtg gttagaatga cctgtttttc
   101221 tcgtacaagt tgtatcacta taataaaccc atcttccaga gctatgatta attgctgttc
   101281 tagaaatttc ccggtctact ggtaaatttg acttaataat attaccttta gcatcaaaat
   101341 gaatctctcc tggctttaaa gatttgtaat agatttcacc gtgaactgaa tttgcttcca
   101401 cttcagatga catagcgttt cctccaacaa aacctatccc tagtgcacaa ataataactg
   101461 cttttttcat aaaacgacat cctttccttt ttgattttta aagcatgatc ttcttgtttc
   101521 ctattttggt cagatatgga tcatgcgtga ctacgataac taacgtacct tgggaagctt
   101581 gttgcgataa gatttcaact actttatcct tatttctttt atccaatgac ccagtcggtt
   101641 catcggctaa aatgattttg ctattcttca ataagactct cgcacaggcg attctttgct
   101701 tctcaccacc agataatgtg tagataggcg tacttagctt cttgtttata tttacttttt
   101761 tcaatgcctc atactttgct gttcgcttat ctgaaacatt taaattcgaa aacttcaatc
   101821 caatatctaa attaaaatcg attgtttcat attcaatcaa cccaaaatct tgaaaaagaa
   101881 aagatatggt atttcttttt atcaagcgct tctctctccc agttaattgt ttcgtctctt
   101941 gatcgaacaa atagaattgt cctgaactga actgatcatt caatcctaat atattcaata
   102001 acgtactttt acctgctcca ctttctccaa caacagtcac aagatcccca gcatccgcag
   102061 tgaaacgatc caaatgaaga atcgttttgt ttcgaatgat ttttttcgca ccttttatca
   102121 ctatcaacgg ctccaattgt taacctccat tcatcagcaa taactgactt ttcttttctg
   102181 tagacaaaat aaaaacaact gaaagaataa aatcaatgaa gctaaagaaa aaaatattaa
   102241 aaagatattt aggctctctt tctataagaa aaaggaagat tacacttgtc gttccccatt
   102301 tgatgaggtt catcatgaag aaatctttat acgtttcaaa gagcgaataa ccacttgaac
   102361 gaaagatagc gattttccga caattgtaga caaaatatat gcataacatg acatagctca
   102421 acaagaagaa agaaaagaaa gtgagtaaaa atatctgtat caagtaatga aatgatctgc
   102481 ttaacacttt gacagctatc tcttcagtct gagtatacgg aaatgataac tgcaagttat
   102541 cacgtaactg actttcttca atcagctcaa ataacctttc tttcgacaaa gagcctatct
   102601 tgatctttaa cggtggatag atttctccgc tgagaatatt tctttccaaa tacatactgt
   102661 ttttcaatgt cagtatttca acaaccggat aatgttcgat ccaggggata gaaggaataa
   102721 atgtataaat gggttgattg ttttttataa atagaaattt cggttctttt tcactgattt
   102781 cttgataata ttctgttatt tgcgccaggt cattttttaa atctgtatgt ctatatcgtt
   102841 cagggatcaa tataacaggg tgcgtttctt tttcggttat ttggatcaac cttccagaga
   102901 catcaatcaa aggaaatttc ttaagataat tcagatttat ttttatccct ctgccaaact
   102961 gttcattttc ttcattcaag taatctgata cattgactaa tatacttccg tttttattca
   103021 aagtttcata taattcctga tctgctgctg atgcaaaacg attggaataa ataaaatcca
   103081 cgtggttttt cccgatggtt actggataaa atacatgata gtcctcacca gcgatttctt
   103141 ttggtggttc atgggaaaca gctaactgtg aagaagaaaa aacgatagtt gctaattcga
   103201 cattggcaac ggtcaaaaga agagaaaaca acaatccaaa tgtaaaaaga atgtattgga
   103261 tatagttgaa cgcttctctt aacgaagcga ttttttcaac taataaaaga gaaatcaaag
   103321 agataccata tattccgctg aacaagaaaa cgtacatctg gaacaaataa agaaattgct
   103381 ccttataata cataaaaata aaaccaccaa tgattcctgc aattatgaat gtaataatga
   103441 ttttcttttt ccccaataaa caccacaata cttgatgtaa tgaaaagtca ttattcaaca
   103501 ataaactgat ttctttattc atcaataaga aaaaggatga taacaaaagg aataaaagaa
   103561 tcaatccaat agttaaatac actcgataat tatatacgtc tgtatttgaa ataaccgaat
   103621 acgaatagct attttcaaag tcttcaaaaa tattagtggc ataatcagtg tgaaatgctt
   103681 gattgaatcg atcagtgaat agtgaaatga accgttcata ggcttctttt tctctcgtca
   103741 tcaagaaaaa ttcaccttca aaatctttta ttttgatcga ttcttcaatg gacgttacgc
   103801 gaaaggaggt attagaatag ggtaccttct cgtgagaaga tttgacatcc ccttgataat
   103861 acaatgtcat ttcaagcttt ggctgtatct tgaagttcca agatgtatct acttcattgg
   103921 attcatttac tgtttttttc atataagaaa gctcagtttc tgatgagaca ttttcaaata
   103981 ccatgattag ctgctcatat tcttcttgcc caataaaatg ttgtggcaat cgaatcggag
   104041 tataagtatc tgtttgatgg aaaaattcat ttgtccgata gagtacctgt ttatttatac
   104101 taaccaaggc aaaaccactc atgataccgg tcactatgat tgtgagtaaa actgtgaact
   104161 tttttttgat tatcaccact ctcccttact taaatgattc tttgataaaa tcataccgtt
   104221 tttcatcaaa aaaacgtatc tatcaagtgt ataggtcatg tagaataaat ctatatactg
   104281 tagaatcgaa aggagaagaa gatgaacacc ggagaaaaaa tacgaactta ccgcagaaat
   104341 tgtaagctta cgcaaaaaga attaggcgaa aaaattggtg tttccgataa aacgatttct
   104401 agttgggaaa attctcgtac aatgccagac cttgaaatgc tgtcactttt acatcatact
   104461 ttaggtctgc cgattgattt tccttctatt gaatcagcta ccgaatcaat agaaccaaca
   104521 ttgccagatg aacctcttgt taacaagaaa tcttattcat caaaacaacc cttctcactc
   104581 aaaaagacac tatctttctt tctgctcgtc ttttgtttgg tctatctttt tcttatcaat
   104641 tatcaataca aaaatgaaac gatcgatcga ttcaatcctt ttttgaaacc acaaatcggc
   104701 tataccacac tccctgtagg atacgtggac aattccatcg aacgttgggt atgtaatgat
   104761 atctttggcc atggtacatg gctgtctttt cacgctggag aggtagaaaa agagaaaaac
   104821 catgctgtaa tcaaacacaa aggaaaacac gtcatgaacg ttcgcctgct cacttgggaa
   104881 gccattccct cagattacca agaaatcctc agccaagact atgacgacgc cttaaaaaga
   104941 agccctcagg agccagatag gcattaagta ggagatgaac gtagagatag aaaaaatcaa
   105001 tgcacatgat cttatccaaa aaacaggggt acaaattaat tgaaaaagcc ttgagatgaa
   105061 agttcctaaa taaaaaagtt cagaatgata ggtatttctg gcttatagta taatatttac
   105121 gccacagcct cccaatgatt ctttttggaa tcataatcaa ggatggacat tcacaaaaat
   105181 attttgctca ttcgatccga atagattaac gaccaatcta gtaagtaata tagattggta
   105241 cgtagtcaag ctgcacataa tttagatcaa ttttagttct tttttatatt tctatgatct
   105301 gctcatttac ttttatatga tggcttacag taacataaat aaatatgata atcgctggta
   105361 gtttatccaa gagtactcaa taaaatacaa aatggattgt tcaagcgcct atcataatga
   105421 taaaatgaaa caaataaacg atacaaggag gaagagactt tgagtaatgg cagaacaatt
   105481 ttttcattgt aaaaattgtt cagttgttaa tctatgggat ttcggtagca tgggcatttt
   105541 tttatagagg aatcacacca acctggccgt tttatctctt gttagtggga atattagtag
   105601 gaaattttgc ccctcttcac tatcgtacga acttttttcc aactgattct ggcctttttc
   105661 ttaaaacaaa gacgcgaaaa ttagaaaaca ttattgaaat gacaattacc tgtataatca
   105721 ttttcttagt tatattccta aacgtcttct aataagaatc tcttaaaatt agcttgttac
   105781 agttagttac ctagtttata taacacagtt atcttatctt ccttatcaac atcttttgcc
   105841 aactctttgt cttaaattcg aaacatataa gcagagctac gaacaccaag actgtttcgg
   105901 ctctgttttt tgcatcaaat tcactcttat atagctgata atctcggctc tttaaaagaa
   105961 attattttcg cttcatttta ttataaaaag gctaggaacc ttgatgttat caggttccta
   106021 gcctttattt aagtattacc ccacagaccc ttccatatag atttttaaat tacacactag
   106081 tttaaactaa aacaaacaag gaaaaactta ccatttaatc aaaaaattag ctcatatgaa
   106141 tatattagaa tgtaaattag actggtcaaa atctggtcaa aaaagaggac gaatacaggt
   106201 agtcctcttt tttgttattt tgatattcta aatcattttt tattggatta ttcccttgtt
   106261 cgatatagac ccaaattgat ctatcaataa ttatcaaact gagtcatata gagacaaaaa
   106321 tttcaaaaat gacctgttaa atggtcatcg aatatcattt aaaatcaata cgacggagtc
   106381 agacacggag tcaaaacaag cccgctgaga gtaaaaaata tacagtccca taggtaaagt
   106441 gccacaggac tgtatatttt tttatttgag attacctgaa atgattccat ttaattttta
   106501 tctcattttt tcctactagt tagtatcaaa tactctccta ttttctcaat aaactatttt
   106561 gggttacatg gtatctgaaa cagctaaaac cttgtcatta tagcaaaaaa tcactttatc
   106621 tccgtttcat ttcagataca tttcacttaa gcggattcca aaaaaacaaa agaaaaagga
   106681 acccctagtt agcctatgct ttttattggt tcctctcaaa acacggtcat ataacaacgg
   106741 ggtggggtaa atgaatgaaa aaggtggagg aaacattatt caatagctgt cttaaatact
   106801 tcgttataaa atgtgaacaa ataaaagaca tctatcccta ttttctacac ttccacttga
   106861 ctaccatctt tgaatcgtac aattttctta actcttctcg tttacctcaa ttcattctca
   106921 agtggaatca actcatcatc taacatttta cgccatgctc gcttcatttc ttgtgggtat
   106981 ttaaatggct tcgcttctgg atgtttggct ttatatcctt cgtaatcctt actgttgata
   107041 attgctttca tattaggaat tggatcttca cctggtagat attgcagatt agaagcatgt
   107101 aattctttct cagaaacaaa ccactcattg gaaaagacat gaatcaaatc atcactcgct
   107161 tgtgtaaaat agcgacgttt aatgacaaag atatcttcat tactatctaa agtccccaca
   107221 tcaaatgcat ttaaaatttg tcggtaaact tctttgactc cttctgtttt attcaattta
   107281 gccaaagctt tttcaatttc ttctcgtgca tctgaactat tcgcagcata agttcctagt
   107341 aattgatcaa tataagttga atcaatatca tacaacttag tggtattttc actataaaat
   107401 tcgatcgttg aaaaatcttc tccttctttt tctggttctt cctctattaa tgaacccttc
   107461 acggtctcgt acacgccaat ttgttcttcc aaaagttgaa tttgattgtt caaagttgga
   107521 gatttcacca tgtcatcatt gtaatcgtca taagtcacta acgattcata agcacgattc
   107581 aattcttgaa atgctttgac gtactcgata cggatttcaa gtggcgtatg ttcatcaatg
   107641 gattccatgg atgaaacctc ttttaactta ccatatgctt gtttgaatct ttttttggat
   107701 tcctgataac ttggataaat caaacctgat tcttcttttg cttttgaata cagttttgtc
   107761 gcatcctcga cattcttctc cattgtcgct ggcttacgaa acgtcacaat caatcctttt
   107821 gtcttatcag gatacgtacg attcgtacgt gaaaaagctt ggatcaatcc tgcatactct
   107881 agattacgat ccacaaatag ggtttgaatc gtaggggcgt caaaacctgt taatagacga
   107941 tccactacaa tcgctaaatc gatttgtttc ccaaattgtt tgaattccgc tctttttcgt
   108001 gccaaacgat tattgatgtc tccgttataa cgctcaatgt ctgctaatga ccaggaagta
   108061 tcataatact gattatattc ctcaataatt ttttgcattt cttcttgttg ggcacttgaa
   108121 tctcggctat tttcttctaa tgaataggta atagcgattc gtggaaaatc aggatcctcc
   108181 atcgttcgtc cttcacgaat gagctgattg ggaaactctt ttgttaacca atcaggctct
   108241 ttcgtcatct ctttgatggc ccgataatac tttttggcca tgtcaatgga actcgtggtt
   108301 aagattgcag atttcgttgg cctgccattt cgaaaatcaa attttgtata ggcattatct
   108361 ggacggaaca ttttatgaat gactttttgg atatgttcat ctgcttcaaa aacagctggg
   108421 tccaaataac tttctttttt gacaggttcc atttgatcaa tcatacgatt gatctgttct
   108481 gatgaatact ctgcatatgt ttctacttct tttaatttcc gatagatttt attctctagt
   108541 gaagtcggct ccacggtgtt ttcatgctct acttgaaatc ctaagacaga gccatcttct
   108601 agcgcattct taatggtata ggtatgcaag acctctccat actgatcatg ggttgtacgt
   108661 gccaattgcc ctttggcttg tttccgattc tcaggaaaaa tcggtgtacc tgtaaagcca
   108721 aaccaagtgg atttcggaaa catttttttg atttcttcca tattttcagc acttaacgct
   108781 cgatgacact catctactac aaacacgata tgttggccca ttaatttttg gaagcgattg
   108841 gttccttttt tctcttcttg tttctttgca tatttcaacg ccgcatctag tttttgacgg
   108901 gttgtaatga ttacaacatt tgcattggca tctgccaaca gggtttcact taattctttg
   108961 gcacttcccg ttccaacgat caagctattc gctttggcgt tcccggaaga aatcccagta
   109021 ttaaattccg aagcaaattt tgtaaactct gtggtggttt gattatctaa atctttgcgg
   109081 tccactaaca tgatcgttcg atcgatcccc gattttcgtg ctaaaagctt agtagataca
   109141 aaactagtca acgtctttcc tgaaccagtc gcatgccaca cataccctga ttgatgttta
   109201 tttgcggcaa taaacaaggc ttgaatcgca tgtacttgat aaggatgtaa caccatgagg
   109261 gttttattgt cttgatcttc actgacgatt gtataatccg caatcaaacg atgagcatcc
   109321 gggatattta acacttgttt acaaaactca tataagtttt ctactttttc attgtctttc
   109381 gttcgccaac taaataaaaa cttagggtgc aagtctttcg gtaaggcatt tgcaaaatac
   109441 cgagtggttt gttcattgga aaccacaaac aactgtaagg tcgaaaagat attgttccga
   109501 aacatacctt cttctgcata ttttttgatt tggttaaatg cctgataaac accatctttt
   109561 gcagttactt gtttcagctc gatttggaca atcggtaatc cattgattaa taaggtgacg
   109621 tcaaaacgtc gatcccgtgc ttctatgtta cttccacgtt ttgcaatttg atggaccact
   109681 tcataagtag agattcctcc accaatgtct tgattggaat aaaggataag tgagacagaa
   109741 cccaattgcg aatcttcccg ttcaatcgtg atccgagcca tcccattttc accttttaac
   109801 catttcgcag catcaaatgg ggttttcgtt cgcagcagta attccgtttt gatcgtttca
   109861 aattctttat cggtcaaagg aaaatcattc agttctgctt gattatttga gataattttt
   109921 tggcgtaaat tgacccaaag gtcttcttct gattttagat ccggacgata cgtccactga
   109981 ttatgccctt ctccaagtac acgaatcaac tgttcttcga cttctgcttc ctcacggtga
   110041 ctaattttca ttcttcttcc ctcctagaca aacatctttt gtaagaagct ttttttcgtt
   110101 tcttttagta aatctaactt acgttgatga agagcgatcg tatcatctag ctgtttgaag
   110161 aaagtaccga ttttttgttg ttcatccttt acaggaacct taacactgat attctcaata
   110221 tcagaactgt tataagaagg caatgcccct acttgggagt atcttggcaa atttatagtt
   110281 tcaaacaata ttttaccaaa atagatatcc caagtattat taaataaata agccatggta
   110341 ttgttatcta ttaagaaagg gcttgtgacc aaacgttttc tattaagcat tattgctgcg
   110401 ccaactttag caaatataat cgctggcaca tcttttattg gcttccattt tttacgctca
   110461 atttgctcat tagatacata atgatttgca tttctcattt catactcatt accaatgtta
   110521 ttcatgtcgg aaactttaaa aaatggagta ccttcagaac caccttgctc ggcatcagga
   110581 aatccaattc ccgattgagt tttacctaat tctcccaact tacgctcttc ccaatcttcc
   110641 gtaaaaccag gaaaccggat ttctggcact tttgctccgt tttcagggaa cattttttgt
   110701 aagaatcctt ttttcgtttc cttcagcaaa tctaacttac gctgatgaag agcgatcgtg
   110761 tcgtcaagtt gtttgaagaa ggtgccgatt ttttgttgtt cttcctcact aggaaacatt
   110821 gctactatat ttttaaactt ttttagagac agaacatgtt gacctgttcc ctctgcttga
   110881 atagataatt gtcgttggat tccattgtta atacgtctta agaagaagtc ggaatcagca
   110941 gattttaaag taaatacagg ataaaaatag cttataatcc ctctatcaat aatgtcatta
   111001 cggttaaaca caaacacatc attatcactt cgactacgga acgtgaaata tcctctaggt
   111061 agtacaaagt aaccaatatt attttctgta gttacttgcc gatttgcaaa atagtcttct
   111121 tgcaaaacta tccctttttg ttgagatgaa gttagcatag gaaattgatt ttgtacactt
   111181 gtcttttcaa cataatctac aacgaagtcc cccaacttcc tctgttccca atcatctgta
   111241 aatcctggga aacggatttc tggttgtgta tcattgctca tgtttgaaca cctccagcgc
   111301 accttgtaac cacgcttcat tttcaggtgt tgtttgcagg gaagagatag cttctaaaag
   111361 ttttttttct agcacttgct gttcttgtcg tatcttcttg atgtcatttc cgacatgaac
   111421 catatcaatt ggttcttctt cttcgaaagt atctacatag cgtgggatat ttaagttata
   111481 gtcattttct ttgatttcat caaaggttgc caaatgagcg tatttctcga catcttttct
   111541 ttcagcgtag gtttcgagga ttttttgaat gttttcttcc gatagtttat tttgattttt
   111601 ccctttaaca aattcacgac tagcatcaat gaataataca tctcgagttt gacgattctt
   111661 cttcaacaca atcaccgtcg ttggaatcga tgtcccaaag aacaagttcg caggcatacc
   111721 gatcacagca taaatactgc catcttctag taatttttga cggatgaccc cttctgcagc
   111781 gccacggaat aaaactccgt gtggtaagac gatggccatg gtacctgttt ccttcaaatg
   111841 atagaaccca tgaagaagaa acgcaaaatc tgctttggat tttggcgcta atttcccata
   111901 acgattaaac cgagaatcat ctaagaaagt cgtatctgct gaccaattag ccgaataagg
   111961 tgggttcatt actactgcat caaatgtgta aggttcatct gttggccagt ccttattcaa
   112021 cgtatcccca ttgcggagat tcatttcttc ggcgtcgaca ccatgtaaaa tcaagttcat
   112081 ttttgctaga ttgtatgtcg ttgtatttag ttcttgccca tgatatttaa cgttatctgg
   112141 gtgtgttaag tagttacgta cgtttaacat caatgaacct gaacccatcg ttggatcaaa
   112201 aacactaaag aatcgtcgtt ccttttgatc gagcgttacg atttgcgcca tcatatcaga
   112261 aaccatatga ggtgtgtaaa attctccggc tttcttccct gcttctgaag caaattgact
   112321 gatcagaaac tcatacgcat ccccgatcac gtccccatcg tgttctaaaa catctacatc
   112381 attcaatttc ttgatgactt cggtaatcgt cacatttcgt tgttgctcat ctgttcctag
   112441 tttttttgat tggagatcca catcatcaaa taacccatta aattgattgt aggtagatgc
   112501 caattgcaca aaggctttgt ttagatcgtt caattggaac gttgcttgtt ttgcttgatc
   112561 cgctaagacg ttgaaaagat actctggtgc aattgcatat cccaaaatat caacgattgt
   112621 ggcaatcaga tcttcttttg attcttcatc tgaaagtaat tcacgataca acattgtttg
   112681 tttgcttact gtgtcatatt cctctaagga ttcatctgct aaaagaacca cttgttccaa
   112741 caatcgatcg gataaatatt tatagaaaat cagtcccaat aaataatttt tatattctga
   112801 cgcatccatt ttactacgga gattatctgc cgcactaaat aatttttgat ttaattctgc
   112861 acccaaaaag atacactcct atctcttatt taaagttgct atataccctc ttattttagc
   112921 ataccccctt ttataaaaag caaaaaaaaa aagaaagaaa cttattcttg ctaataaact
   112981 acgcgaaagg agtctaacgt tgtttgatat aacttagact gatcgttaac ttatttttta
   113041 tatttctaca tagttatctt tagaaccatt tcagatacat atgaaacaat tacatatgat
   113101 tttttcataa aaaaacagta ctgtctagta gtataaaata tacaaatttc ttaaaaattt
   113161 tttcactaaa atcactacct gaaacagtct aaaacactta tatatcaacg tttaatgaaa
   113221 ctatctccgt ttcatttcag atacaaataa atatagtcgg gctccaaatc cgaaaaaaaa
   113281 tacccccagt agcttatcct aagctactgg agatgtataa gtacggtcat ataacaacgg
   113341 ggtagggtaa ataaatgaaa aagatggaag aatcattatt caataacttt tttaaatact
   113401 tcgttgtaat cagttacctg tccgttctta aataccacag aataatcttt tttcccattt
   113461 tcaagtaacc gaagtggaat gtctttacca acttgctctg tcactgcatt actgatacct
   113521 gttagattag ctaataaatc tgataaagat tgagcatctg tagaaatctc tgtatcagta
   113581 tttatcgtga ttcctttatc ttcatttaca agcttccaag taggtggtat agctgtgtat
   113641 gtttcgatga atgaatcaat tctttcatct atcgtcttcc cagtatctac ttctgtctca
   113701 ttactcttag atgaatcgat tctttcatcc atcgtctgct ccgtaactgc ttctgtctca
   113761 ttattctcac cggaaactct cttcacaccc aaacttatat attgtccatc tttagtgtgc
   113821 atagttaaaa ttcccacgct tttactaata aattcactat aaccagttct ttcaatagag
   113881 acttcaatgt atttcacact atcatactca aaagaaaaat agacatgtaa aagcattttg
   113941 ttgtttacat ctacataata atcctttgac tctacttgag accttcctat acggaataca
   114001 gctttactgt ctgatatctc tataccagca tccttattat taatctgcca atctccaagt
   114061 agctcttctg gcaagctagt agtttcttgt gaacacttta ccacttcact ggtagaggtc
   114121 gaaacattca cctccgcact gttagaagaa ttttccaagg aatcaggagc cttagcacaa
   114181 ccaacaagtc tttatatatt gtgcagccga tattttcttc attcagtaca cccttgaaat
   114241 atgcagtgtt agataaagca gttcgtcctg atttgctgag gcacccatct cttcttgaac
   114301 gtactccttg attttcaagg ccgcccgata ctctttcgga aaacgctgct tcactgaagc
   114361 cagcaaaaaa tcatccttct ccggcaactc ttccttctta atcattcggg caataaaaaa
   114421 ctgtaaatgc gtcacaaatc ttaagtaaag catcgaatct ttatcaacct ttttttcggg
   114481 aaacagatat cctagcatag ccaagatatc ctttagtgct cttaccgaga gcatggtttc
   114541 ttccatattt gcttgttcac tttgggcatt tacaatgtga aacgcaatgt ttccagcctc
   114601 ctcttctggc aactgaacat tcattttttt gttgatcaat tggatagcat agcagccgat
   114661 ctcaaattct tttgggtaat acttttgaat ttcaatcagc aggcgatttt gaaaaacaat
   114721 gcctttttga ctgcgttcaa tggcatgctt caagtgatca accaaggtaa aaaatagact
   114781 tttattcagc ttaaagtcaa acttggcgtt ggcataatcg ataattcgct tgacgcaaat
   114841 aattagatca tccggagcag cctcaaacag tttaatgtaa tcctttttta gagcatcaga
   114901 aagtgaatct aatttaaata ctttttggat tttgtcagcc ggaaccaaat cgcctttctt
   114961 tagttgaaat ccaatccctt tccccatcaa aattacttct ttgccttgct tatctaagca
   115021 aagaattaaa ctgttgttta acgttttaat tactctcatt agattcaccg ttctctctaa
   115081 gctaagccat ttcttgcgat tactttttga taccaaaaga agctttcttt ttttattcgt
   115141 tttaattctt taagattctc atcggttcgg tcaacatata taaaaccata acgcttattg
   115201 aagccttgat gggtactaac aacatcaatc gctgaccaag gtaaataacc aaaaacatct
   115261 acaccatctg ttaaagctgc ttgaatagcc agaatatgtt tggctagata atcaattcga
   115321 tcctggtcat taattcgtct atcttcctca actatgtcgg gtgccccata gccattttca
   115381 gtaattaaaa taggtaaatc atagcgatcg tacaatttcc tcaatgttaa tcgtagtcca
   115441 gtagggtcaa ctacccagcc atatggcgtt ttatctacat aaggattttc agctgcccgg
   115501 tagacacctt cttctcccaa catgatctgt tgatcccccg ctctagcgga aacatctgat
   115561 ccgtcatttt tgctggcagc gatggtggca gttgaataat aattaatcgc aataaaatcg
   115621 ggtttaccgg aggataaaat tgcctgatcc tctgcctcaa ttttaggata taatccacga
   115681 tcattcaaat agttttcaaa caaataattg tattttcccc gaactgctac atctaagaaa
   115741 gaccagcctc tcaatgtttc ccaattatgg gctgcaattg catcttctgg actggaggta
   115801 gcttgataca tagccgttaa attgattgct ggaccgattt ttcctttaag attcaactga
   115861 tgatacaagt gaattactct agcttgagct aataaaacat ggtgattaat ttgaaagagt
   115921 tctttttttg aaggcagctc tccacttggt ggcaaaccaa tcgctccagg atgaaggatc
   115981 atcgtgtttt gctcattaat tgtcagccaa tagttcacct tgtcaccata ctcagtaagc
   116041 aatacctgta catagtccac aaaataatcg atagttgctc gatttaacca accgccctgc
   116101 tcctctagaa aataaggtaa atcgaaatgg tacatggtga caatcggttc gattccctga
   116161 ttcaggcatt catcaattac ttgatgataa aaagaaattc ccttttgatt ggttttatta
   116221 ttttcaatat ccataattcg cgtccaagcg atggaaaagc gaaaagcttt taagcctaat
   116281 tctttaaaca gctgtatatc ttctttatag tgatggtaaa aatccgaggc aacttcaaaa
   116341 ttagctaccc cttctgtatg ctgatacttg tcaataatag acggttgttt cccatcctcc
   116401 tgataggcac cctctgcctg gtaggcacta atggaggcac cccataaaaa cttctctgga
   116461 aatgctgaaa gctgtttgta ttgcacaaaa atcctctcct taactaattt gcatcaatgg
   116521 ttcgtgttcc tttaagcttc cgcttgcttc actaattcct gcgtaatttt cactgttggt
   116581 cactaccaca atcactgtat catcgtagcc agcgtttcta atggcagcga aatcggcgtt
   116641 gattaataat tgtcctgctg acacctgatc tcctgctttt acaaatactt caaagggttc
   116701 cccttttaaa ttcacggtgt cgatccccac atggatcaat aattcaatac cttctttaga
   116761 acgtagacca atcgcatgtt tagattctaa aatagtaatg acttctccct caacaggtga
   116821 ataaatgctg ttttcttctg gatgaatggc tacgcccttt cccaatagtc ccttagaaaa
   116881 gacagcatcg ttcacttcag aaataggaca cacggttcct tttaccggat tttcaacaat
   116941 gactgcttcg gctattgccg tttgctctga ttcatcagaa gtatcgtcaa atccaattag
   117001 ataagcaatg ataaaggttg caacaaaggc gattacacac gtaataattg cgtgaacaat
   117061 attcattgga ttttctccaa taaaggctgg taaggctgct aagccagggg aaacaaaggc
   117121 atatctgact aaaccgctca agccagcata caaaccagca attgcaccac caatcatgga
   117181 agcaactaat ggttttttca gcttcaaggt tactccatag agtgaaggct cagtaattcc
   117241 cattaaagca gtgatcccag tggacacagc tagctgcttc aactctttgt ttttagtttt
   117301 cgcggctaca gcaaaggtag cacctgattg agcaatatta gaagccagca ttcctggccc
   117361 attgatcatt tcagaacccg tactgctcaa ttgcatagta gcgattggcg tcattgccca
   117421 agctgtaccc gtaaccacca aaaatggctg caaaccaccc atcaaaaatg gaattaacca
   117481 agctgctttg ccgttaatcc attcagcccc cattgctata agatcgttca gcaacgttcc
   117541 aaacggtcca atgacaacca acgctaaagg ccccataatt aaaaatacca gcatcggctt
   117601 agtaaagaat ttaataaacg aaggcgaaat tcgatccgca aagggctcta cataggacat
   117661 gatccaaaca gtaaagataa tcggtaaaac tgatcccgca taatctgcag ctaataccgg
   117721 aattcctaga aaagaaacgg catcgccagt agaaaaaagc tgtgaaatat ttggatgaag
   117781 caaaatgcca gcaacagtaa tcgccaaaat tgggttgaca tcaaacttta tggctgcccc
   117841 ataggctaat agtactggca taaaataaaa ggctgcatca gcaatagtat tcaaaatgat
   117901 gtaagtttga ctactgtctg atagtacacc agtcaaagtc aagacggcca aaatggcttt
   117961 taccatcccg gcacctgtta tagctggaat catcggtgta aaggtggtag aaatgacact
   118021 aatcaatctt gaaataatac tttcttgatt gtctgaagag gggccctttt taattgaagt
   118081 tcccacttcc ttttgaattt ccttgaaggc tgtttggact tcattgccaa tcaccacttg
   118141 gtattgtcca ccgctatcca ccacactaat tacaccaagt gttttttcta aggctttcgt
   118201 atcggctttc cttttatctt tgagttcaaa acgcagtctg gtggcacaat gggttaagct
   118261 gaccacattt tcttttcccc caacctgttg caatacattt cttcctactt ctctgtaatc
   118321 cattattctc tctccttaaa gcagtaaaaa agaccaagtc aaaccacgcc ccaatcaaca
   118381 ttgaagtgtt agtttgtctt ggtcttgcct gcataatcag taacatgcca ataacgttat
   118441 tttgctgtaa tccaataata gcacattatg atagcgcaat caacagggaa tttaattttt
   118501 tatcttttcg tttgaccgta tagggtaata taacagttag atcacatcaa ctagggtata
   118561 tctgcgtata cttactgcta aaataagtat atctaatatt aactatacca gtaaaaccat
   118621 tcctacataa ccagcgggaa attcccaaaa tcgtgaaagc atcaaaaatg catataacgg
   118681 atcacacaac ttcaacaatt ctgctcttgg gaaaaattga tgaatttggt gagtaaagga
   118741 tggaataaac agcattccaa caatataggc aacaatagtg atacttgttt ttttggtaaa
   118801 aatcagtaag caatagccaa tttcaatcat tccgagatat aaaggtatcg atagaatgat
   118861 ttgggctagt attcttaaaa attaactagc aaatgtttaa actgatttat ttctaatcta
   118921 catgtagtaa gtatttcaat tccaccaatt atcaataaaa ataccaaaca tatccataat
   118981 aattttttct ttttcatttc ttttatattg ttataacagt gaataacata aaaattcccg
   119041 acaatcattg taatattttt ttgtcacctg tattagtatt taatttatag gaaactataa
   119101 aaactcccta gtgaatttac acctttggga gttcctgttt ggacggtatg cataatacat
   119161 ttaaatcaat ttaacattaa cacctcagca tatggtatct ccacatatat acggtctttc
   119221 tttggatagt actcaatatt tatgttgata gcacttccat tgtctaatat agctttagcc
   119281 cggtatgtgt tatctccatt ttcatgagta atcgtcacat catcacatga tcgatttaag
   119341 ttgtactgtt cttgtaaaat ctgtgttacc aatggttttg atgacttctc taatccagat
   119401 gtacctgtca tttttgaaaa aataatacct ataatagcca agacaacaat acctataatg
   119461 acaaacttct tgttcatatt tactttattc aactttttat ctcctatctc caaaataatt
   119521 tgctcttatc cctcttatca gtacattgac atccctaaaa gtacaccaaa gattgctcca
   119581 gctacccctg ctatcatcaa aatcgtaccc attttctcat ctttttctcg atataagtag
   119641 cccataataa cagcaacagc accaaataca acaggaataa atgctaacga tactaccata
   119701 gaaatccaac caataatcaa atacagcttc cctgtctctt gtccatgtac gatttgttcc
   119761 ttcatttcat taattgtctg ctcagtactt ttctttactg tgtctgttcc ggttgtctca
   119821 tctgtttctt gtagtgctcc acaaaactgg caatacttac tttcatctgt tatttctttg
   119881 ccacattttc tacaatacat gtcttctcct cctaaaatca ttcaattgct tattagttaa
   119941 ctgtttactt tattaaatac atcagaaaac agctcattca ttggtacact taatatctca
   120001 gaaagcatct tcatttgata gggtttagga atcgttctcc ctgtttgcca cgaacttaca
   120061 cattgctgtg taacattcat tttttttgct aactccgttt gagaaagtcc tttttctttt
   120121 ctgtatcggc tgagattact tacttttttc atgattctcc cctcctgttt tattatatac
   120181 aacaatatga tgtatttcaa ctcataaaat ataaaaaact tcaaatacca ttacaaatac
   120241 aacaacttgt tgtactatac tgagatgggg gtgtattaat gaaacagaaa caatatgcaa
   120301 aggtcattag agaaaagcga aaagctctcg ggtggacaca aaaagaatta gctgaaaaaa
   120361 tcttctctac acaacaggca gtagcaagat gggaaaattc tgtaacagag cctaacctcg
   120421 atagcttaac agctctttct agagccctag gaacccctgt aagccatttt ctagacaatg
   120481 ttgtcgtaga ttatgaggaa gagtttttgg ctctctatcg ttctcttagc actgaagatg
   120541 cagttcgaac aattgactat atgaagctat taaaaaggca agaaaatgaa cgcaatcagc
   120601 tattgaaaga ttgaaaaata aataacccac cgaactttaa cggtgggtgt attgagatga
   120661 aataagatta agagctatgg ataacctgtc tagtttaata caattgggtc ttttctcttc
   120721 cttactctcc gtccatcatc tcttgttaca cttgtaaatt gatacagagg aactatccca
   120781 gctcctgata atatcaattc ttttattgtt tcatctatcc ctgccccttt cgctaaatat
   120841 attaaaaaac tactacttgc tttcacattg tatagtttct taatatcctt agaagctcgt
   120901 tcaagagtga atccttgttt aacaaagtag cctctgtttt ttattaaagc atttttcacg
   120961 ctgttatccc caacattacg taggcgttca caaacagtgt taataagttc tttctctgtc
   121021 attagatatg tcccttgcca cgataagccc tgtaaaactt catgtgcttc atctaaaatc
   121081 aattgttttt ccttgctgag ttctgatcgg gaattttgag gaaatactct tttagcctct
   121141 ttttcgttaa aacatatctt aacccattca taagaaaaat attgatatgt aaaatgaatt
   121201 ttttccagaa gtggaaggat atcttcattc aatttttgaa tcacagaagt tgcattaaaa
   121261 acgtctaatc gatagacatt gcttctctta tgattaaagt ttcctaacgc tttctcttcc
   121321 tttaacctat ttaggtattc gagcatcttc aaaggaacat cttcatcatc taattttttt
   121381 atcaatccta taaaagtcat aagtgatagt aacttattac atttatctat gtcataagtt
   121441 ttaccatagt aagaactcat ttttttagct aattgttccc ccgacaaaat acaagtcaca
   121501 ctttccttac tatcatcaaa aaaattaaca tcagaaagca agacatcata gacacgtcgc
   121561 atttcctcta gataattcct tattgtcctg aaaagtttgt ctttcattga ttcaagagtg
   121621 tcaaaaagta cttttgattc ctctttcaat tttcgaaaat gtgtaggata caagtctagg
   121681 ttctctaata gccaattaaa tgtttctacc cgactgtctt tctttaaaat tagcatcaca
   121741 actccaataa aattttcact gatatcacaa ttactggaat gacaataaaa aatggaatgc
   121801 ccagttttac tggtaaaaat actagcagag ggattatgat cgttgtgaaa cagacatctt
   121861 aagtttttac ttgacttaag taatttattc atatcaatgg ttaacaatct ttcataaatc
   121921 tcattaaaag acatgtcgga ggaatcaaac aggcagtctt taaaccactg tttcatttct
   121981 tctttgtcgt cattttttat caacttaaca aaatcggttt cattgactag ttcatactct
   122041 ctccctgaat cactttgaca atttttagaa catgtcatgg taaaatcaat cggaagctca
   122101 gtaatatcta acatgttgtc ttcattaatt attatcactg catctgactt atatcctcca
   122161 aaaaacattc tattcgaagc atttacattg tcatcacacc cggggatgtt ctttttcaag
   122221 tattcgtaaa cagcaaccat tttaccatag tcagtaattg cttcttttaa tagaaagaca
   122281 atcttgtatt tttctagatt ttcagagtac cgaatacttt tttgtatcgc aaacgcagaa
   122341 ttatgaacta aattgataga taaaaccttt tctacgctta agtatctact atcatcttta
   122401 ctcaaatcta ctaccttccc cttctcatct ttaacagtgt tatcgacatc cagaataatt
   122461 actctggttt ccacaaaact ctccttgtca ttgcctttat tctttgtctt gcttggaata
   122521 aatgattgtg atgaatttag tatctctaca aactcatctc ttgtaacttc tctatagtta
   122581 tctaaacacc tatctcttac atcagttttt tctttttttg taactggctt ctctaataat
   122641 ttttcttgat ctacataaat tggaatatta atcattgatg tttccatttg tgtttcctcc
   122701 ttataaaatt ctgttaaaga ctaaaacata cagataataa aaacaaactc tttattacta
   122761 tatgttttag tcttatttgt ttactcaacc tttataccgt tccatgaaac tatcaatatc
   122821 agatttttta aatctaatga ccgtatcaat ttttataaca ggtaacccgt attgagaaac
   122881 atattttcta atcgttccta ctgatacacc tagatattca cttgcctgct gatagtccat
   122941 atactctaga gtgactccag aaataatgtc ttctagcata ggctttagca ctgtaattat
   123001 ctctttcact tgttgttcaa cttgtataga aacaatgcta tcaatgattt gtagagacat
   123061 atttccccct cctttcgtga ttgcttgaac aaattttcta agaattatct tcgtattttt
   123121 tgctcaaaat aaaagtattt tgttattaac cgattaatca aacaaaaaat gattaccagt
   123181 tcatcgacat acacataata cctcgataaa aaaggggtgt caacagcttt gtaaaattta
   123241 tttgcaacac cccttttttg aggtatacta attttaggag gtgtggtcaa tatgataaaa
   123301 cgtccaccaa taaattattt agaacgaaaa aagatactcg gaacaaaaat aaaagctata
   123361 agaaagagta aaaaattaac acaaccagca tttggtctta tgattaacaa cggtcaatta
   123421 attgacaaaa aaactatcta cgaatgggag aagggaacct atttaccaat accagagcga
   123481 ttatcccgaa ttgctgattt gggaaatatg agtatagagg agttagtttg tggcaatgta
   123541 gaagaatata ttttgggaat tattctttat agagatagca ttgtactaga tggtataaca
   123601 tttccagata aaaacctgtt ccagcatctt cgtcaacagt tcccaccagt acactctaat
   123661 ttagatacat ggctggatag atattccaaa ttagaaccag agatgcaaga atttattgct
   123721 aacaagactt gtaataaagt taaaaatgaa aaaattagtc ttttcaatat cttaaaaata
   123781 gaagaattat tcatcaatgc tattgtagaa gaatttgaca ataacattct tttcctcact
   123841 agcagtatag aagaactact agaaagaatg gtagatgaat ggctacctat tcaattgaaa
   123901 gacatgtctt atcccgaaga agctgtgaga gaaattactg ataatataaa caagctagaa
   123961 caaacgattt catcaattgg gaaaaaatac actaagaaaa aaatgaaggg aggtgatacc
   124021 atctagtggt aagtactcct tgtaaactaa gaggagatga tttaatggca aaaatatcaa
   124081 agtacaaaaa gaaaaacggt caagtggcgt ggatgtataa aggacacatt gccaccgatc
   124141 cacgaactgg tgaaaaaata aatactacga gaagaggttt tagtagcaaa caagaagctc
   124201 aaaaagatta tgacgagtac agacatcaaa tattatacgg tgttaaaaag aaagcacctg
   124261 atatgacatt tgaagacctg tataatgaat ggataactca tcagcgtacc agtgttaaag
   124321 cttcaacaat cgcaatctca gtaagatacg caaataatca aatacttcca gcatttggta
   124381 agcttaaact atcgaatatc tctgtcccat attgtcaaaa agtggtagat gaatggcaca
   124441 gtaaatatga aagttacgat tatatgagaa aacagactgc acaaatttta cgctacgggg
   124501 ttgcaatgca atatattgac aataatccta tggaaaaaac acttcttcca cgaaaaaaag
   124561 agtatgagaa aaatcgtaaa ttttactcaa aagaggagct taataattta ctggatgctt
   124621 ttaaagattt tggtaacatg aaacaatacg ccttcttcag attattggca tacacaggta
   124681 tgagaaagag tgaagtactc gctttacagt ggaaagacat agacacgttt aataaagaac
   124741 tacatgtcaa taaaactctt gctgtagatg aatttggtaa agttataatt caatctccga
   124801 aaacaagagc ttctagacga gttatctctt tagatactga gactctttct atcctcaaca
   124861 actggaaatt acagcaaaaa gaagagtatt taaaacttgg atacaatact agctctaaag
   124921 agcaacatgt ttttacaaca gttaaaaaca ctctatacat tccaaacact gttaatgatt
   124981 ggttaagata tattttgaaa aaatataact tgcctcgtat tacacctcat gggtttagac
   125041 atacacatgc tagtttatta ttagaagcag gagaatctgt caaggtagtt caacaacgat
   125101 tagggcatga aaatagtaaa gtgacattag acatctatgc acatattaca aataatgcac
   125161 ctaagaaaac aggtcaagac tttgctgata tgatggctca tcagtaaaag agctggtcaa
   125221 aatctggtca tttctagtat acaggcaaaa caaaaaagct agaaccctta tatatcaagg
   125281 gttctagctc ctataacagg tttacccaac actgccttcc atctcataac tgatcaatcg
   125341 attgagttcg actgcatatt ccattggaag ttctttggtg aatggttcaa caaagcccat
   125401 gacgatcatt tcagttgctt cagattcggt caggcctcgg ctcatcagat agtagagttg
   125461 ttcttctgag atttttgaga ctttggcttc gtgttctaaa gagacttggc tgttgtggat
   125521 ctcattgaat gggatcgtat cggatttcga ccattcgtcc atgatgatcg tgtcacattc
   125581 gatatgagag atcgaacccg cactgttttt ggcaaaggtt acttgtcctc gatagtttac
   125641 ttctccgccg tcttttgaaa ttgatttaga gacgatagag ctagatgtat ttggtgcatt
   125701 gtggatcatt tttgcacccg tatcttggat ttggtttgct cctgcaaagg cgatggagag
   125761 catggtgcct cgtgcacctt ttccatctaa gtaaacactt gggtatttca tcgttgtttt
   125821 tgcacctagg tttccgtcga tccattcaac ggttgcgcct tcgtatgctt ttgctcgttt
   125881 ggttactaag ttgtacacat tgtctgacca gttttggatc gttgtgtaac ggcaatacgc
   125941 atcttttctt gtaaagattt ctacgatggc ggcatgcagg ctgttacttg aataagtagg
   126001 tgccgtacaa ccttcgacat agtggatgct tgctccttcg tcaacaatga tcagcgtacg
   126061 ttcgaattgc cccgtgtttt ccgcattgat ccggaagtac gtttgtaatg gaacatcgac
   126121 tttgacgcct tttggcacgt aaataaaggt cccacctgac catacggctg aattcagcgc
   126181 tgctaatttg ttgtcggttg gcggtacaag ttttgaaaag tactctttga ataattcagg
   126241 gtattctttc aaagcagagt cagtgtcagt aaagacgatt cctaactttt cgaattcatc
   126301 tttcatatta tggtacacga cttctgattc atattgtgcg gaagcaccgg ctagataagc
   126361 tcgttcagct tctggtatac caatcttttc aaatgtatct ttgatttttt ctggtacgtc
   126421 ttcccagtca cgagcgggtt tgtcacttgg tttttggtaa tatttgatgg catcgaaatc
   126481 gatgtctgaa aggtcggggc cccatgtttg cattggcatt ttgttaaatg tttctagaga
   126541 tttcagacgg aattcaagca tccattctgg ttcgcctttg attcttgaca tctcacggat
   126601 cacttcttct gtcaagcctt ctcctgtact gaaaaccggt tcgacatcat cgtgaaaccc
   126661 gaatttatat tcttctaatt ctggtacgcc catgttctca ctcctcttcc ttgtggtaat
   126721 gatggaaagc tgcggtacca tcgttttcca ttgcttttcc tagtgctttc caggcaagtg
   126781 tcgcacattt gatccgagcg ggaaatttag ctacaccacc aagcatagca gcatcgccta
   126841 attggtcggg atctttgact tcttttcctt ggacaagctg tagaaagtct acagttaaat
   126901 gttctgcttc ttctattgat tttccgatga cggcgtctgt catcatcgag gcacttgccg
   126961 tactgatgga gcagccactg ccgtcaaagg cgatattttt gatgattccg tcttttacat
   127021 cgacttgcaa ttcgatcaca tctccacatg tggggttgtt caattcgatc ttgcttgtcg
   127081 agtcagtcaa gcttccacga tgatgcggat gggaagaatg atccaagatc acttgacggt
   127141 ataaattgtc taatttagat agtcccatgt tggaaaaact cctttgttgc catgattgct
   127201 tcaactaaac ggtcggcgtc ttgcgttgtg ttgtagaaat aaaagctcgc gcgagcagtc
   127261 gctgatactt caaggtactt caatagcggt tgcgcacaat gatgacctgc tctgacggct
   127321 accccttcca tgtctaaagc tgtcgcgaca tcatggggat gtaacccatc caaattgaat
   127381 gcaacgactc ctgtccgaaa attcgggtct tgtggaccat aaacagtcaa tccatcaatt
   127441 gcttgtaatt ttggcaacac ataagcggct agttcttctt catatcgatg gatattgtcc
   127501 ataccgagtt tttgcaaata atcgattgca tggcctaatg cgatagcgcc ggcgatattt
   127561 ggtgtccctg cttcgaattt ccaaggtagt tctttccatg tactgtcgta taattcgaca
   127621 aaatcgatca tttcgccacc aaattcaaca ggttccattt cttctaacca ttttcgttta
   127681 ccatataaaa caccaatacc tgttggtccg cacattttgt gtccgctgaa agcaaagaaa
   127741 tcacagtcta gtgcttggac atcaatggcc atgtgcgggg cagattgagc accatctact
   127801 acgatgaccc cgccgttttc atggacaagt tcagctaatt cttgaacagg attgaagaca
   127861 cctaagacat tggatacatg agcaatggag acgattttgg ttttgtccgt aatctttttc
   127921 gctgcatcct ccatatccag gaagccttct tctgttagtt cgatatattt caagacagca
   127981 cctgttcgtt ttgccaactg ctgccacggg ataacattag aatgatgttc catgtaagag
   128041 agcactatct cgtctcctgc tttgatatag cggtcgccga aggttctggc tacccaattc
   128101 aagcttgtcg tcgtaccacg agtaaacagc acttctgctg tttcttttgc atggatgaac
   128161 gcccgtactt tttctcttgc ttgttcataa tcatgggtcg cgcgttctgc taacgtatgg
   128221 acaccgcgat gaacattggc gttatcgtgt tcgtaatagt acgctaatgc ctggagtact
   128281 tgttttggct tttgggtcgt cgctgcatta tccagataaa ctaaaggttc atcattgacg
   128341 tcttgaaata agataggaaa atcattgcgt agctttttag catcgatcat ccattcaact
   128401 tcccttcgat tacttcgact aattctttcc gaacttgttc tacagggatt gctgttaaga
   128461 cagaaccgag gaatcctcga atcactaagc gctctgcttc ttctttatgc aatcctctac
   128521 tcatcagata gtacatttct tctggatcaa ctcgtcctac actggcagca tgtccagcag
   128581 ttacttcgtt ttcatcgatc aaaagaatag gattcgcatc accacgtgct ttatcagaaa
   128641 gcatcaagac acgactttct tgttgggcat ctgcaccttt tgcccctttc aagatgtggc
   128701 cgataccatt aaaggtcaat gttccttttt ctcggatcac tccgtgttgc aaaatatgcc
   128761 cgatcgtatg aggggcttta ttggttactc gtgtatcgat tccttggatt tgtctgcccg
   128821 agctgattgc cacgattttg acttctgcgt gtgcaccttc tcctgctaaa tctgagtcaa
   128881 agtcagcgat cacatgaccg tcattcatga caccaattgc ccagtcgaca gaagcatctc
   128941 gtaaaatgtg cccacggcga ttcatataag aagtaatatt ttctcctaat tgatctacgg
   129001 ctgaatattt gatttttgaa ccagctttag cgatgacttc cacgatgata ttacctgatg
   129061 attttgctac ttgttctttg gttgtctgga aacgttccag ataagaaaat tcactatgtt
   129121 catctgccac gatcaacaca tgcttgaaga agtgctcatc ggatgcgcca tcttgaatga
   129181 ataaggactc gagtggttca tcgatcacga catttttagg cacatacagg aaaataccgc
   129241 tgttcataaa agctacatgg agtgcagtca attggtcttc gttcatgttt actgcttttt
   129301 tcatataata ttcttggact agttctggat attcgatcat tgcagtgaac aagtcggtaa
   129361 aaatgactcc tttttctgct aattccacag gcagttgttc aaaaatcgtc catgaacctt
   129421 gttggacaag cacaggattg tctttcattg catcaaatgc cggaatcgtg cctgtttcac
   129481 tgatggtttg tgtttctttc acatcaaata acgaccagcg atggaatttc acccgatcga
   129541 tatgcggcaa tggcagttca tctgctttcg ctagtgcgtc ttgacggaag gttctcatcc
   129601 aatcaggttc gcctttactt aaagaaaaat catttaccgc gtcaagataa tcgagccagt
   129661 ttttttcttt cattcttgct cccccttacg cttcttcttc tttatagtcg attcctaatt
   129721 ctttactgat accagcgtag ccttctgctt ccagacgttt agctaggtca gcattccctg
   129781 tcatgactac tcggccttcc atcatgatgt gaacgacatc aggtgtgata tagttcaata
   129841 atcgttgata gtgggtgatg atcaatgcac cgaaattgtc tccgcgcatt tcatttactc
   129901 ctttagcaac gactttcaaa gcatcgatat ccagacctga atcgatttcg tctaaaattg
   129961 caaatgtagg ttctaacatc aacaattgca ggatctcatt ccgtttcttt tctccgccag
   130021 aaaaaccttc gttcagataa cgttcagcca tttcttctgg catatttagt aaagccattt
   130081 tttcatctaa ctttttcaaa aatgccataa cagaaatttt gtcgttttca tcacgtttcg
   130141 cattgatcgc tgcacgcatg aattccgcat tcgtgatacc tggtatctca cttggatatt
   130201 gcatagctaa aaataagcca agtcgcgcac gttcatctac ttccaagtct aaaacatttt
   130261 tcccgtcaaa caaaatttcg ccttctgtta cttcatagtt tggattgccc atgatagcgg
   130321 cagataaggt agattttcct gtgccgttag gtcccatgat ggcatggatt tctccggttt
   130381 tcattgttag gtttacgcct ttaaggattt ctttttcctc gatcgacaca tgtagatttt
   130441 ttatttctaa gacagacatg cattataccc ccaaattctc tttcgttctc actcatttta
   130501 gactaattga gaatgaagcg caatgacctt ccttactaaa atcaaatatc ttttatttat
   130561 tattaaacaa ttttgatttc taaattagaa taggtataaa aaagatgaga aataagccat
   130621 tacagactta tttctcatct tttttgttcc ataccttatt ttgcatcagc ctttactggt
   130681 ttaacagagc cgccccattt gtctgtgatc cattcttgga cttctttgcg gtgaagcact
   130741 tcaatcagtt ttttgatttt gtcactgtct tcatcacctt tacgagtcgc aataatattc
   130801 acgtaaggag agttgtcctt ttcaagcaat acggcatctt ctaatggatt caaccctgcg
   130861 ccgtaagcga agtttgcgtt gattgcaact aggtcacctt catcattttg gtacgtagaa
   130921 gctaaaagtg atgggtcgat cgtatggttg aacttcaagt tcttagggtt ttcttcaata
   130981 tcatcgaatg ttgctgtttc cagatctact ccatctttga ctttgactaa gccagcatct
   131041 tgtagaattg tgatgatgcg tccccagtct gattcagaat ttgaagtaat gattgttgca
   131101 ccatctttta attcggaaac atctttgact tttttagagt acaagcccat tggttcgatg
   131161 tggacagcac cggcattgac aaagtcatat cctttttctt tgatttcttt gttcaaataa
   131221 ggaatatgct ggaagtaatt ggcatcaatg tcgccttctt ccaaagattg gttcggtaat
   131281 acatagtcat cgaatttgac gatttcaaga tcgattcctt catctttcaa taatggtttc
   131341 acatgttcca aaatctctgc gtgcggtgtc ggtgaagcgc cgactttcaa tgtcgtgtcg
   131401 tctgctttat ttgctgaatc tttgctgtcg ctgttgttac cgcatgctgc taatcctaca
   131461 gtcagcaaaa gcgctgctgc aaatccaaaa attttctttt tcatatccta catcccccta
   131521 tatcctatta tcttttgtct gttttcttag ttaagaaatc accgattcct tgaacgataa
   131581 atacgattag taaaataatt aaagtcgcaa cgaatgtcac tgttagatta ccacgctggt
   131641 agccttcctg ccaagcaaga cctccaagtc cacctgcgcc gatcgcgccg gccatcgcag
   131701 taaatccgat catcgagatc gttgtaactg ttaatccaga aatcaatgcg ggaatacttt
   131761 caggtatcaa gaccttccag atgatttccc agtaacttgc tcccattgca tctgctgctt
   131821 ccagtacacc agggctgact tcacgaaaag caatctctac tagacgggca taaaatggtg
   131881 ctgcggataa aacaagagct ggaatggctg cttgcgcacc tagcatagtg cctacgattg
   131941 ctttagtaaa tggaatgatc aaaaccatca ggatcataaa tggcgttgac cggaaaatat
   132001 tactgatgat cgatacgatt ccataaatga ttcgagcata cggtttgttt ttgcgtccaa
   132061 tagagtaaag gatcaatccc aaaactaatc cgatgatcgt cactaagacc attgagatta
   132121 atgtcatata tagtgtatca aggatagcct gattcatcgt ttccgggttg atttgtttaa
   132181 aatctagata tgtttctgaa aagcttttat ccacgatgga tcacctcaat tcctactcct
   132241 tgttctttga tgaacacttc tgcttgatct aattctttgg tttcaccagt catcaagaca
   132301 gtcaatgaac caaaggaacc ttctttcact tgtttgatct ttccatacac aacgttgaca
   132361 tcgatgttat agcgtcgaat cgcttgggaa atgactggct cgttggcatt tctttcgcta
   132421 aaggtcaaag ttgccaaacg gccgcttgga ttttctttga tgagttcagc taatagttct
   132481 gtcgaatcag cttctggttc gatgtcttgt tgtacaaagc gtttggttac ttcttttttc
   132541 ggttgtctga agacagtcaa tacgtctcct tcttctacga ctttccccaa ctccatcaca
   132601 gcgactttgt tgcagatttt gcggatcaca ttcatttcat gtgtgatcaa gacgatggtc
   132661 aaattcaatt tcttattgat cgctaacaat aagtctaata cttcttctgt tgtttgcggg
   132721 tccaatgcgc tagtagcctc atcacaaagc aggatctcag gatcatttgc caatgctcgt
   132781 gcgataccga cgcgttgttt ctgtccgccg gaaagttgtg aaggatatgc ttgtccgcgt
   132841 ccttctaacc cgactaaacg caacagttct tctgcccgtt ctttacgttt gctctttggt
   132901 acgccagcta attccagcgg cagctggata ttttcaagta cagttcttga ccataacagg
   132961 ttgaaatgct ggaagatcat accgattttc tttcggaatg tacgtaattc tttgtttttt
   133021 aattttgtaa tatcttgatc gtttacgatc acttcgccgc tcgtgggaag ctcaagtcca
   133081 ttcaggagac ggactaatgt actttttcct gcgccagaat agccgacgat tccataaata
   133141 tccccttttt caacgtttag tgaaacgtca ttgacggctt ggatgtttcc gtttttccca
   133201 ttgaatgtct ttttgacatt cgttagttgg attaatgaca tgccattcac atctttctct
   133261 tatgattttt caaacaaaaa agccttcgcc ctcttgcctt aagcaaaagg acgaaagctt
   133321 gaacttccgt gttaccacct tgatttaccg ttacttcgca ataacagcct cttccagtac
   133381 ttggacttag cttctttccg tatactgtcg cgctgtaacg ggcactcccg tagtaagttt
   133441 gcttacgctc gcttactctg ctctaagacc atcttccatc tcattttcgt tacccatttt
   133501 cagcaaacag ggctctcttc aaacgtccgt gacatgtact cttcttttca aagctttctc
   133561 gtttcatata ggattgctta tgattatagc aactggaaga atcagtgtca acttttttca
   133621 agtgattttc tgattttata attcgtcaaa ttcttttgga tcaacatctt tgaaacttaa
   133681 gatccacgca ttcatttcat ctgaatcatt caagatattg atatcttcac cgattttatc
   133741 attaacagaa gaaactgtac ccgttagcgg gctggcaaat tcattgaccg ctttttccgc
   133801 ttcaacttcg atcagaggtt catctttttt tagcgattgt cctgcttttg gcagattggc
   133861 aaacgtgatg tctccaagtt cttcttgcgc ttcatttgtc aaaccaacac agtattcttt
   133921 tccattaaat aagatccaaa ggttgtcctt tttcttcaaa tgcttgcctg ccattttata
   133981 tcagttctcc ttcataaatt ttttcattga atccaattgc aacaagctgg tcctctcgta
   134041 agatcagtgg tcgcttgatc agcataccat ctgtcgataa gacttggctc gcttttgaag
   134101 ccgtaaactc gcctacttga tctttcagtc caagttcccg atatttcatc ccgcttgtgt
   134161 tgaacaggcg acggattggt acttgactcg tttctagcca agtctcaatc gttgaggcct
   134221 tcggtacttc tttgatcata tcaacggttt gataactaac gccatgctgg tctaaccacg
   134281 ctttggcttt tttgcatgtt gaacatttcg ggtaccagta aaaggtgtac aattttctta
   134341 cctccttgaa gatcttgcag gtgcttcatt ttggtatctc atcgataata tcaacccgat
   134401 accgatcatg ttccctaaaa tcgaggatcc tccctgacta atgaaaggca atgggatccc
   134461 tgttaaagga agtaatccga tatttgctcc aatattctca aagacatgga acagcagcat
   134521 catgatcaaa ccgcttgcga tataggcgta aaattcatta ttcgtatcga aacacacccg
   134581 aatcatacga tagattaaaa taaaatacaa gaaaatgacg aatgcaccac cgataaagcc
   134641 gaagttttca ccgataacgg taaaaatcat atcggactca cgaacaggca catagatatt
   134701 gcttacgttg aatcctttac caaacaagcc accggtacca attgccaaaa gtccttgggc
   134761 cggctgataa ctcattcctg acgtatcatg gaaagggtcc agccatgaat caatacgtgc
   134821 aaactgataa gatttgaatc caactttata aagcaagtct cttccgcctt cagtcgtaac
   134881 caagaagatc gttcctgctc caacgactac tgccaagatt actacaggtg cgatgatgcg
   134941 ccaagagatt ccagacatca ggaaaatccc gccaaagatc gcaagaaaga caagcatcgt
   135001 tccaaagtca tcttgggcca tgatcaatgc taaaaccggt attgtcacga tcaagagctt
   135061 cccaatcagc caaaagtccg atttcatcgt ccgttctttt acttgcgtat tatgtttagt
   135121 tacgatcagt gccatcatca agatgtacgc aatcttcatc aactctgctg gctggaaagt
   135181 aaacgcaccg aatcgaaacc agtttttaga ccctgttgaa gcagctaaat tctgatcata
   135241 gaatttgagc aagagtccca ttaccactaa ccccaaacca taaaggtaag gagtcagttt
   135301 ccagatccat ttggaattca aatgcatgat gacaacgatt gctgccgctc caactaaata
   135361 ccagacaact tgcatcccta cgcctttcaa gactgctgga ttgttcggat catggctaag
   135421 tgctacatat aatgagagca gaccaatcaa acataacaga aagactggta aaatcacgcc
   135481 ataatcaata cgattatcgt tcgaagcttt taattttttt tccatgtttc atcccttttc
   135541 ttaaaaacca tactgctgat tattatagcg gtccagttag ataatggaaa gtctttccat
   135601 agacatttgg gaaaaattaa ctttccgttt gtgcagttgc ttgagcaaat gcagtacaaa
   135661 agcctactgc ttatctttag tgtatcatag aacttttttt gatacatgca attatctacc
   135721 agctgagatt ctcctcgctg tgagaagttt ctgtgctttc attgtatcga aggatctctt
   135781 cggcaatctc tagcagctga tcagaaacca gattggttcg ttggatgaaa taaaaatatc
   135841 gatggaaatt caacggagtc attggcacat tttctggaac agagcgctta gacaaaatcg
   135901 agcgaccgat tccttccttc agcaacgcaa caattacatc gttacttttc acgatcatct
   135961 tcttatcttg gatattcatc tcttcaaaat aacgctgcgt gtaatgaaaa acaccagagt
   136021 ttgcttctct tacaagccac aatttgcttt ttgggtcccc agcgatgact agctggtctt
   136081 ccatcaaaga gtatctttgt acgccggaag tctctaatgg cttttcgata aaaccaaaat
   136141 cggattcatg tttcgctact tgttctaatg attcatgaga attcgtgatt tcgatctcaa
   136201 aatgcacttt aggaaacctt ttgatcagat gtttcatcaa ctgaggcaaa agatagacag
   136261 caaaggtgtg ggaagcaacg atccgacata cttcttcttc attttgttca ttgagcaatt
   136321 tttgctgagt ttcttcccat tcttctaaca aaaccagtgt ccgttcatat aataagtcgg
   136381 cttgcttagt tggaagcatc tcttttcttc cgttacgttg aaaaagatcg atattcaatt
   136441 cccgctccaa ctgtttgatt tgattcgaga ctgctggttg agataaaaac agtacttctg
   136501 ctgctctgga aaaatttctt gtttcatata ctgctcgaaa agtcgttaat aacttaaaca
   136561 tggcaccact tccataactt tttatgataa ttactataaa aactatttga tttaaatata
   136621 caaaaaacca tctataatag aaatgtacaa caaaacagtt gctgatccaa ctagtaactt
   136681 agatgaagac aatcagctgt ctgcttgctc gtaatcatac attcctattc attttacaat
   136741 gtattcgttc atcttggtac taaaatctat taaataaaaa ggagaatatt atatatgaag
   136801 aactttttga aaattattcc gatcccgatc tgcgggttga tccttgggtt agcttccctt
   136861 ggaaatctgt ttaaagacta tcaccatgtt gctttaggca atctaactgg cggtatctct
   136921 atgattctta tgatattcat ttttgttaaa ttgtttatct tattcgaaca cacgaaacaa
   136981 acgttgcatg atccaatcat cgcctccgtt tcaccaactt ttacgatgtc actcatggtt
   137041 atttgtactt attttgtttc atatgaaagt attgcaccaa ttgtcaaatt catttggctg
   137101 tgcgctgtta tcttccaagt gatccttgtc ttttatttca attatcacca tgttatcaaa
   137161 gctgatcttt caattgaagc aatttatcca agctggttta tcatttacgt tggatttggg
   137221 gtcatcactg taactgccgg gaacttcttc ccacttattg gaaaaatttt cttttggatt
   137281 tcattgatct gctatgcgtt cttattgccg atcatcatca aacgtgtctt tttcgtaaga
   137341 aatatggctc atccgacacg tccgctgatt acgatcgttg cagcacctgg ttctctttgc
   137401 ctaacaggtt atcttaaaaa ctttgctgaa cctaattttt atcttgtcgt agcattgttc
   137461 gctttatctc aagtattgta tttcgttgtg ctgtctatgc ttccacgttt gttcaagctt
   137521 cgtttttatc caagctatgc agcctttaca ttcccgcttg ttatctctgc aacagctata
   137581 tttactactg ttcattactt caacagttta ggtatctata gtagcgtatt ggatttctta
   137641 aaagttgcgg aatgtctgtt tgcaagtgta gttgtcttct acgttcttgc tcattacgta
   137701 cgtttcttaa ttaaagaaca tagcaaacat ggaaaatttg tttctaaaaa aatcgcttaa
   137761 taatcgattc aataggttgt gaaaaagctg ttctgcatca agtaataaga acggccaaac
   137821 aaaaacagct gttcatgttt ttgtactggt tgggcttatt accgcagatg caagcttttg
   137881 aacaccgttt accaagaggt cgtaaaagaa gtggtcagct tcaagaaata agaaggaatt
   137941 tacgaaaatt gcttctcaaa tttttgtgaa atttcggctt atttccgtag aagctacttc
   138001 tggaacaccg tttattcgaa acaagaggtc gtacaagact tttgtcacga cctcttgttc
   138061 ttttgtcaaa atactggttt agtgctacta tgttcgagta tgcttttagt atttattgac
   138121 gaaaggaacc gatcaaatga aatcattgtc acatccaata aaatctgtaa aggattcagc
   138181 ggtccgcgaa atcttacccg gtttactgct ttcttttgcc atcgctgcct tcagcaggct
   138241 tcttgctatt tatctgccac agttaggtgg agcgactatt gctattttat tgggtatttt
   138301 attaggaaat acttttttta aacagcctgt tctagcaatc ggcacaaaat ttgctgaaag
   138361 caaactatta gaattctctg tttttttact aggtgcaact gtcacagctc aaacgattgc
   138421 tcaaattggt gtaaaaggaa ttgtttttgt gttactgcaa atgtcagtca ctatcatcgg
   138481 tgcttacttg attggtaaaa aactgcaatt cagcgatact tcttctttga tgatggctgg
   138541 aggaaacgct gtttgtggtt cttctgctat tgcttcaatc gccccttcca tccatgcgaa
   138601 agaggaagaa aaaggacaga tcatcacact agtcaatcta ctgggtactg tgatgatgtt
   138661 gactctccct tttcttggat tacagttgtt tggtggaaat ctcattgaaa aaagtgcact
   138721 tttgggtggt acactccaat ctgttggtca agtagtcgct ggggcaagct tacttgacaa
   138781 ttctgttgtc gaattcgcca tgctatttaa gattctacgt attatgatgc tcgttgttgt
   138841 tgtctatctg tttgaaaaat tcatgcacaa gaaacaacca gctctgtttg aaacgccaac
   138901 tgatacgcag aaaaaacgaa aattaccgct tccttggtat gtattagggt ttgtgatctt
   138961 ttgtttggtc aacagcttgt tttctttacc tgtagttatt gaccaatcgg cacattttat
   139021 cagcagctgg tttgagatca ctgctttagc ggcaattggt ctgcgtttag actttgcaaa
   139081 attcatgaaa gaaggtacac gcttcttatc ttatggatta gctgtcgggc ttctccaaac
   139141 agtgatggct ttactattta tttggctgat caaagtttag aatgaacatg aaaaaacagt
   139201 tgtgattctc gcatggaaca atgtccgaat cacaactgtt tttaattttg cagatatcct
   139261 tctaacaaca acaattggcg tttgatttca accccatcta ttgcgtttcc catatatcct
   139321 gtcagttttc cattttgccc gatcactcga tgacaaggaa taacgattgg caatggattg
   139381 gttcgatttg cctgcccaat cgctcgcaca gctttcggac tatcaacagc aacggctact
   139441 tctttatacg ttcggatttc tccataagga atggtcgaca aagctttcca gacattttgc
   139501 tgaaaaggcg tacctctctt tatagataaa ggaacatcaa attcttttct ttttcccgca
   139561 aaatattctt caaactgttc ttgtgtgcgc gttaaaattc gctgattcgc tgattcgctt
   139621 tctgaaattg gctgaaaaga tacagcagtc aatccggatt catctgcatc taaccataaa
   139681 aagccgatag gtgtgtgtat tttcattttg tttgctccta gtcaatagac tggaacttcc
   139741 tcctttttct ctatccaaaa ctacttgcca actaataaaa aatgttctta aaagtttgat
   139801 aaaccgagga aacatggtaa actaaatacg ttagtttaca aaaagaatac ctgttctttt
   139861 acgtaaaatc aaattttaaa ggtgttattt aaggtttatt aaattttgaa tgtaaaccat
   139921 atataaacat acgattgaaa ggatagaaac gatgaaagta tcaattgttg tcccatgttt
   139981 taatgaagaa aaaactgttc ctcttttttt tgatgaggtt gaaaaattca aaggtgccca
   140041 tgagttcgaa tatttgttta ttgacgacgg atcaaaagac ggtacgttag cagcaatcaa
   140101 gaagctagcc tcagcttatc cttatgtccg ctttgtctca ttttccagaa attttgggaa
   140161 agaagcggct ctgtacgctg gattgcaagc ttctacagga gatttagtga ccgtgatgga
   140221 tgttgatctg caagatccgc ccgaactgct tcctgagatg atcaaccgga tcactacttc
   140281 tgatatcgat tgtgtcggca cccggcgttc gactcgtgaa ggcgagcctg cgatacgcag
   140341 tttctttgcg aaaaaattct accagttgat caataaaatc agcgatacag aaatggtcga
   140401 tggcgctcgt gatttccgct tgatgactcg tcaaatggtg gatgccgtcc tagaattaac
   140461 agaatacaac cgattttcaa aaggattatt tagctgggtc ggatttaaaa cagaatatat
   140521 ttcttatgaa aaccgcgaac gtgtggctgg cgaaacatct tggtccttct ggaaattatt
   140581 caactactcg attgatggta tcgtgaactt ctcggatgca ccgttaacga ttgcttcatt
   140641 tatcggcgct ttgtcttgtg tcggttctgg tattgcaatt ttgttcatta ttttgcgggc
   140701 attgttgttt ggtgatccta catcaggatg gccttccatg gtctcgatct tcctgttcat
   140761 tggtggaatc caattgttat gtcttggaat catcgggaaa tatattggga aaatcttctt
   140821 agaaacgaaa cagcgtcctg tttatatcat aagagaaaca gaaaaagtga agccaaatga
   140881 attgaagcct caccaataat atacgtcaaa tgaagtaaac atttttttaa atgaaaaagg
   140941 actgtgacaa aagtcttgtc acagtccttt tttccgaata aatggtgttc cagaagtagc
   141001 ttcttcggaa ataagccgaa atttcacaaa aatttgagaa gcaattttcg taaattcctt
   141061 cttatttctt gaagctgacc acttctatca caacctcttt catatcttat tacttcttca
   141121 ttgctgcttt tagtgctgct gctaatgggc tttcttctgg ttcttctgct tgtgcatatt
   141181 ttttcagcaa gcgtcgttct tcatgtttgg tcattttctt cttctgctct tttttatcaa
   141241 gcaatttttc tgtcgttcca tcgtatttac aacggaaata agcaccgttt ttaccttcga
   141301 tgatttccat tttccggtga cattgcgggc atcgatgatt agagactttt ggatcttttc
   141361 ttctgcggta gttgcaatct gaattggtgc agacatagat tttgccgtca cgtgtgtttt
   141421 tctcgcgcag attggcccca cattctgggc atttcttttg ggtaatcgag aaatcttgat
   141481 attcttgttt gcttgatttg atctcattca ccaagcgttt tgtttctttt tcgattccat
   141541 tcaagaaggt tcctgcttgt ttctttcctg cagcaatagc ttcaaggtct ttttcccagc
   141601 tttctgtcaa atctggtgtt actaaagagg gatttaccaa tactaataat tgcttacctt
   141661 taggagatac ttgcagtcct tgaggggtac gttccatcag ttcagatttg attagtttct
   141721 cgataatctc tgcacgcgta gctggggttc ccagactgta tttttccatt tgaccaagca
   141781 agcttgcttc tgacaatggt ttaggcggtg cagatagttc ttttttgatg gcgaaatcgg
   141841 gattgattct cattcctttt tcccatttga ctgtttccgt ctcagtttca gtttcgcctt
   141901 tccatcctgc ttggacgaca cgattttgcc gaaaaatgaa tcgttcttta ccaaattcaa
   141961 ccgtaacttt tgtttggctg actttatggg gttgagcaaa cagtcctaaa aaacgagaga
   142021 caatcatttg atagattttt cgctcttcat tacttaattt ttcataacgt gctctgtttt
   142081 cagttggcaa aagtgcatgg tgatcggtca ctttactgtc ttggaatact tttgtctgtt
   142141 ggacgatggc tccgtttttc aaatactgct tgacctcagg agcaaagtcg ctgactgctt
   142201 gcaatcgttc tttcatcgta cttttcatgt catttgtcag atatttgctg tctgtacgcg
   142261 gataagtaac gatcttatgg gtctcataca aactttgtac aagacttaat gttttctttg
   142321 cagaatagcc atagcgttga ttggcttctc tttggatctc tgttaaatca taaggcaatg
   142381 gcgcagattc cgtttttaat ttttcttgga cttctgtgac agttcctttg cttgtactga
   142441 tcaattgaac aagtttttgt gcttcttctt gtgtttttaa agcaaattga tttttttgaa
   142501 gcattttggc ttgttctttt ccagcttgca acgtgatcgt gaaatacgtt tgtggacgga
   142561 actgctcgat tttcttttct tggtctctca ctaatgctag tgtcggtgtc tgcacacggc
   142621 ctgctgataa attatccgcg tacttcacag ttaaggctct ggtcacattc aatcctacca
   142681 gccagtccgc ttttgcccgg gcaagagcag attcatacaa tgcatcatat tctttcgcag
   142741 gtcttagttg cttgaatcca gttttgattg ctttatctgt ttgagaagag atccataacc
   142801 gcttcactgg tttattgaaa tgcacatact ctaagatcca gcgagcaaca agttcccctt
   142861 ctcttccggc atctgtcgca atgactgctt ctgagacatc ttttcgctga gccagctgct
   142921 tgattgcttt taattgatgt cctgttttag gaagtggttt gattcctagt tttttaggga
   142981 tcattggcaa cgtgtccatt tgccagctct gccattcctt attcagatct tccggcattt
   143041 tcaatcctaa taaatgacca agggcccaag taacgatcac cttgggtcct tcataataat
   143101 ttttgtgctt ttgattagca ccaagcacct tgcttaggtc tttcgcaacg cttggttttt
   143161 ccgctagaat tagttgtttc ataaaattcc tttctgcttt ctactgtgat gggctgatga
   143221 tttttcaaca acgcaagtcc cttgtattgc tgtaaatcat cgtcacactc ttcacaccag
   143281 cgctcatctc cgtcattcca aaaggcgctg agaaagttac tttttgcatt gatatattca
   143341 taattttgat ggatttcgtt ccattgtttt tcgtacgctt gtcgtgcttc ttcgaaagac
   143401 tcgaatgttt cttcttcttc aatatcttct tcccagtctt caaaaaacca ccaaggctca
   143461 ttgtccccga acatggtaat tacttgatac atactcgtgc tccttctttc agcttttcta
   143521 gactcatctt agtcactttt caagaaagtt gcaagaattt tctcttataa aagggtcatt
   143581 taatgaacat cttaaaattg ttacaaatga atctgattgt ttaaggcaaa gtagcgataa
   143641 agttattgat catccaaaaa tagctgtctt ctggatcgat tgttggttct tttgtaaagt
   143701 atcctaaaaa acgtaaggaa agataaccgt ggatcatact acgaagttcc ctgcttcgat
   143761 gaactttttc ttctttcgtt aattgaaaag cctcaaatat ctgcaaaatg atctgattcg
   143821 tttcatggat tccatctaat aaaacagctt cattggtatg cggtacactg atcagtaact
   143881 catacactgc ttgatttgaa aaagcaaagg atcgataagt ttctgcatag atctgcagtg
   143941 cttcctgacc actttttccc acaagagccc gtcgcagctg gtcattaagt tcctgcagaa
   144001 gtttcgctgt tagcgctgtt ttgacatcac gaatattttt gaaatggtta tacagcgagg
   144061 gagccttgat atccaagcgt ttggctaaat gctgaaaggt tatttgctga acatccatct
   144121 cttctgctag ttctcgaaag cagtcgatga ttttctcttg agacagattt ctttttttca
   144181 tttccatgtt tcctctcata aaataaatcc ttcactcttt cagtttacca cgaaaaagaa
   144241 agagaaaaaa gagactttca ttgataaact acatattgta gtttataata aagagctgta
   144301 gatgaaaatt ttcagaaaga aggagtctaa ttggctgcaa gaataaagga aaaattattt
   144361 aggaaaagag aaacttggga acaaaacttg tttgtcttat ggtttggaac atttatggct
   144421 ggtatcggat ttagtttagt catgcctttt atgtcacttt acatagatac gttaggtaat
   144481 tatagtacgt cacaattgaa cttttggagc ggattgactt tttcatctac tttcttggtg
   144541 actacgctta tctctccttg gtggggaaaa ttagccgacc aaaaaggaag aaaattgatg
   144601 ctgttgcgtg cttcattagg aatggcaatc gtcatttctt tgatgggttg cgtgactagt
   144661 gtgtatcaat tagtcgcatt gcgtctgctt caagggattt tttctggtta tatcagtaat
   144721 gccactgccc ttgtcgcaac tgggacgcca aaagaaaaaa gtggtcaagt tcttggaacc
   144781 ttagcaactg gttctgttac aggaacatta cttggaccgc ttcttggcgg gatctctgct
   144841 tcagcgtttg gctatcgccc tacctttttc attacaggga tcatcttatt tttcgtcttc
   144901 ttgttgagtc tgtttttcgt ccatgaaaaa tttgtcccag tgaaaaaaga aaacatggtt
   144961 tctgccaaac agattttcaa agacttgaaa tatccacacg tcgtcatcgg tatgttcgtc
   145021 acaacgatga tcatccaagc atccaacaat tccattagtc caatcatcag tctgtacatt
   145081 cgccaattgc tgcatggaca tgggaatgtt acgttgatca gcggaatcat tgcttcgata
   145141 cctgggattg ccactttgat agccgctcca agatttggtc gcttaggtga caagattggg
   145201 agtgaaagaa tacttgcgat tggtttgggt tttgcgattc tggtttatat cccaatggca
   145261 ttcgtcacga atgtttggca attagctgcc ttacgttttt tgattggtat ctctgatgct
   145321 tgtctgctcc ctgctgtcca agcattgatc acaaaatatt cgcctcatca agctgctgga
   145381 cggatcttca gttataacca atctttccaa gcaactggta acgtagtagg gcctatgatc
   145441 ggctccagtg tttccagtgc atttggttat cgtggcgtct ttctttcgac ttctcttctt
   145501 gttttggtca acttcttgct tgtacgacac aatacacaag agatccatca gcaagaaact
   145561 gcagaagaaa aagcttcagg agtctccata gcttcagggc actctccttc tcatagacaa
   145621 taagcgtaaa tgaaaaacac tgcttgaaca gttgtcacag actgtccttg cagtgttttt
   145681 gtttaggcct gttttttcaa gaactcagat aagaggaata atcccttttc caaggtttct
   145741 tttttacaac agtaacctag tcgggcatgc ttcggtaaat caaaggcact tcctggtact
   145801 aatagtactc ctgtttcttc taataaattg atacaaaatg tctggtcatc ttcttcgatg
   145861 gctaatttaa tgaaagaagt agagacgtaa ttgggagcga caagttcaac tttcggttca
   145921 ttggctaccc attgttttaa aatcacaagg ttctctgtaa tgatcttttt gtttcgcgcc
   145981 aatatttttt ctttattctt caatgcatgt acagcaagat catctgacag gactccgccg
   146041 caaatcatcg tgtagtctcg atactttcga aaaacttctg ccaactcagg tccagtagct
   146101 gtccagccaa tacgaatacc tggaatggag taggttttgg atagggaatt ggttgctata
   146161 cctttttcat agacatctac gatcgacaag aattcacctt tatccgttaa aggcgcatat
   146221 acttcatcta tcaatacata tgcatctact gtacgagcaa tttctgcgat tttttctaaa
   146281 gttttccgat ctagcaatgt gcctgtagga ttgtttgcac tattcaaaca gatgattttt
   146341 gtttcaggtt tgactaaggc ttctagctgt tctaaatcaa actgccaatc ttcttcttcc
   146401 ttcaaatgaa caaagtcaac agcggcacct aaagatttgg ggatgtcata taattgctga
   146461 taagtaggaa gcatcgaaac aacatggtct cctgcttcca ctaacgcgta tagagctaaa
   146521 agattagcac cagtcgctcc attcgtttgt aagatgtttt ccggatcgac ggtttgataa
   146581 agagaagcga cttcttgttt gaaaacatct gatccttcga tccagccata gtccaatggt
   146641 tcgtttttgc gtttttcgaa aaatttactt acgctagtcc catctaagcc aacgagttcg
   146701 tctaatgtca atgcttcgat cgagctttgt gcaatatcat ataccgcttt cttttcccat
   146761 ttatttagcc attcttctac accgaaattt gcgattttca tgtttctttc ccccttatat
   146821 cttgatccag cctacagcaa gcaacccgat tgaaagcagt gcggccacac aaatcagccc
   146881 aatcattact ttttcttttc ccgtaatttg atgtttggtt tcacgacaag cgatccagta
   146941 gaagcaaaaa ccaggaagat aggcaatact acataaaagg acataagaaa atcctgctaa
   147001 aatgatgccc atcaattcaa aaactacagc aatcccaccg atcactactt gttttttgat
   147061 tccttctttt aaagaaaact ggacttgata gatcccgaca agtagataac aaatcaaaat
   147121 tgccgctgta cataaagaat aggcaaattg ataggcataa tcagtaacta agaaagtcaa
   147181 taagaataga ttagtcaaga ttgcagtaac aactaacgaa taagttgggg catttttttt
   147241 attcgttttt ccccaacctt tcggcaacag accgtctcga gccatcaaca tcgttgtttc
   147301 tgctgggagc atcgtccaag acaaccaaga accaatgatc gaaataatca agccgatatt
   147361 gatcagcgct gctccccaat ttcccacgat tccttttaaa atatttgcca tagcaggttg
   147421 tgaaatagtt gctaactctt cttgagataa gacgccgtat ggcaagatcg aagcaaggat
   147481 atagataaac aataaacaaa gcagaccaat gatcgatgct tgctgggctt ctgaacgttt
   147541 cttcgcgcgg ttggctaaaa cacttgcgcc ttcgatccct acaaagaccc acatcataac
   147601 catcaaacaa cctttaatct gcaaccagat cgttccttgc tgccctgttc cattcactag
   147661 attatcagat acgttacccc aaaaatcagc agtgaatata ttcactttaa atgcgataaa
   147721 tacaaatatt aaaaatagga ataatggcac taatttacag atcgtcacga ttgtattgat
   147781 gacgctcgct ccttcaactc cattgtttac taataaagtc agaccccaaa taaaaatact
   147841 tgcaaataaa atagaaggaa cattttgtcc acctttgaat atcgggaaaa attctcccat
   147901 ggcgctcatc agcatcgttg caaaagcaac atttcccagc cacgcagaca accagtaacc
   147961 ccaaccactg atgaagccac ctaatttacc aaatgaagct tccgcgtaac cgaaaatacc
   148021 accatctaga tctggacgct tttctcctaa atgattcagc gataatacca aagctaaaat
   148081 accaatccca acgattatcc atgaaaggat cgctggccct ggagctgcag aactagccag
   148141 atcacttgtg atcccaaaca caccgcttcc gattgacgaa ctgacaacca gagcaaccag
   148201 tccccatttg ctaatacccg tcgatttctt ttccatttcc atcaccctca cttataaaat
   148261 tgaacaaatc acttgcaaaa gattcccttc tattttgcaa ctgctcttgc ttaacaaaaa
   148321 gcaactttat tcatattatt ggatattatg aactttttac acaagtattt caagtataaa
   148381 atgaataaaa attaatttta ttgcaaaaaa acagctatgc tatccaaaag aaaaggtgtt
   148441 tcttttgtta gccatagctg ttttgattca tttaatatcg cttaaacgta ttgtaaaact
   148501 tttgtttctt gatatgcgtg gtcgatcaaa ccaccaccca gacattccat tccatcataa
   148561 aatacaactg cttgtccagg agtgattgca cgtacaggtt cgtcgaatac gacttctgca
   148621 cgattatcat ccaataagcg aactgtcaca ggaacgtctt gctgacgata acggaatttc
   148681 gctgtacact tgaattcttt tggcattggt tcattcgtcg taaagtggat ttcacttgca
   148741 tccaagcttg tggcatacaa tgcaggatga tggaaccctt gccccacata taatgtgttt
   148801 gtcgctagat cttttcctac aacaaaccag ggttcttgcg aatctcctcc gccaccgatc
   148861 cctagtcctt gacgttgacc aatcgtataa tacatcaagc cagcatgttg tcctttgact
   148921 tcaccatcaa gagtgaccat atttcctttt tttgctggca gatagttgct caaaaattgt
   148981 ttgaagttct tttcaccaat aaagcagatt cccgttgaat cttttttctt agcagtcgca
   149041 aggcctgcgc gttcagcaat tgcccgtact tctgattttt ccatgccgcc tagagggaac
   149101 atcgtttttg aaagctgttc ttgtgataat tggctaagga agtaagtttg atctttatta
   149161 ttgtctactc cacgaagcat gtggaccacg ccgttttcat cgcgagtgac ttgcgcataa
   149221 tgtccagtag ctacataatc ggcgccaaga tccattgcat aatccaagaa tgctttgaat
   149281 ttgatttctt tgttgcacat cacgtctggg ttaggtgtac gtcctgcgcg gtattcggct
   149341 aggaaatatt caaatacacg atcccaatat tctttttcaa aattcacaga gtaataagga
   149401 atcccgattt gagcagctac tttcgctaca tccttataat cttccgtggc cgtgcataca
   149461 ccgttttcgt ccgtgtcatc ccaatttttc atgaatattc cgatcacgtc gtatccttgt
   149521 tcctttaaca aaagtgctgt tacagatgag tcgacaccgc cgctcatccc tacgacaacg
   149581 cgtgttttgc tgttgtctgt catgatatca ccatcatttc aattagattc tgaaacatct
   149641 acgagttgaa ggtcgcaact tttcagtatt ttacattata cgtcatattt ctgaaaattt
   149701 catcccttaa ttatcaagtc gtaaaaatac tgttctactg gacctattca gtacacaaac
   149761 atgatagtca accaaaaaat cactcatctt ttgagcattc gatataaaaa attggcatac
   149821 tcataaaaag ggccagcctt tcggcaataa ggaataattt tataaattgt gaaaaactat
   149881 ttttcaaaat tgcttcttat cgcttaaagc tgcacgtctc atttacaacc ttttcttttt
   149941 agatgttatg agttgctcag ataagtgatt ttgtttactc taagaaaaaa gggaggcaaa
   150001 agatacatga attactttat cagtgatatg cactttttcc atgaacgctt attaggaaaa
   150061 aacgactttg ctccgcggct ctttgcaaat gtggatgaaa tgaaccaaca gttgatcact
   150121 agctggaatg cagtaataaa agaaaccgac catgtttatc atctaggcga ccttgctatg
   150181 catccgcaat atgaaaaagc aaatgcagaa attttggaac tggtcactca actgcaagga
   150241 acaatccact tgatcaaagg gaaccatgac agtcgcgctt ttttccgtta tttagaaaaa
   150301 aatgatccta ggcttactga cggaagtccc aaattttatt tttatgatgt aggtgccatc
   150361 gttaaattcg accatcaaca atattacttg actcattatc cgatgttgct cgggcctaac
   150421 gacactatcc gcaatcttca tggacatatc catcattaca gcgtacctat cgagaacgat
   150481 gtcaatgtag gcgtagacgc accggaaaga gaactcttaa aggaaaaact accatttggc
   150541 actccgttat ctgagcaaca aatcaaagaa atctatgaag caaagaggaa gtacgatagg
   150601 tcgtgaaaaa actgccctat atcaagtatt aaacaaataa agataattac tcatattttt
   150661 catttgagta ggcttattgc cacaggaaca ttaggatcag aaatggagtt gtaacaagac
   150721 ttctgtcaca actccattcc atactattcg tctttttgct catggtgttc ttttaccagt
   150781 tttttctgta attctttatc ataatttcgg gcagctgacg ttaaaaataa aacgattccg
   150841 cataataaaa tcccgattcc ggaaaacaag aacatttttt ctacccctaa acgatcagct
   150901 aatggtcctg caaataataa tccaattgga cctgtcaggt tcatcaaaga gttgaagttc
   150961 cctaagacac gacctaaaac attcgattca taactctgct ggatcattgc catcagcagt
   151021 gtattgaaat atggcgttgc acaacctgct cctgcattca agataagaaa gtacaagaac
   151081 ccttgactat cgtttggaac caatccgctt gcaccgatcg tcaggcccat aatgatgtag
   151141 gctataataa taggtttcat tcggtctttc catgttccta taaaactgat cagtgcaccg
   151201 cctaagagca ttccgactgc gtagactact tccactgctc ccgcctgtcc aacggaacca
   151261 ccaaagtaat ctagcgtcat caatggatac agactgattg ctggcatgaa caacagcatc
   151321 acaaatgcac cattgatcgt tatgtaccat aaccctttat tatccatcaa ttgctgcagc
   151381 ccaaattttg catctttaaa aatagtaagc ggaacttcca atatttcagg agaaactttt
   151441 gggatcttca caaaaatcaa taagccgaca ccaaaaactg cccccaatac atctaataaa
   151501 ataaggtaat tgacaggaac gactgaaaac aaagccgcac ctagagctgg cgcaatgatg
   151561 aaattcgctg attggaccat tcctaactgt ccattcgtcc ttgttatgtg agaagatgga
   151621 acgattgtcg gcataatcga ctggatcgtt ggcatttgga atgtttgtgc gaccgaacgc
   151681 ataaacaaag acacaaaaac tagccaaatc gggaaggatt ctgaaatcgt tccgactact
   151741 gctaaaataa gcgcgaaaat cgctacaata atatccgtga caatcaaaag agctttttta
   151801 tcccagcgat ccactagcgg tcctacaaat gggcttagta aaaccatggg aatcattcct
   151861 agtaaagtag caaaactcaa aattgtcgcc gaaccggttt ctcttgtcaa ataccagatg
   151921 atcgcatatt ggacgaccat actagtgatt cctgacaaaa actgtcccga taaaaagaac
   151981 aaataatttc tcttccaatt cgttaaaacg accgactgtt cattttccat aaacatccct
   152041 cttttctttt tcattttgta tagcgtttgc acaacaatca ttatacggga ttcttttttc
   152101 aaaaaatacc tttttcccta aataacaatc gtttcaattt aaatttcttt acttagaaac
   152161 aataaatcag caaaatatta aaaataacaa catgtgttaa atttaaaaat aaaaaagaca
   152221 actaatttgc gtcgtcctta caaagttagt catctaacaa taggccgttt caaaactagt
   152281 tgtcttttta tatccacaga tcattcgact ttttcgaata acccacggct aatcatccca
   152341 tccatcagaa aataaaattt atcttggctc attttctgtt ttggatcttt gaaaatattg
   152401 actgctttca atccatttgc tgtagtgatc gacattttca atgtttgaat atcttttgat
   152461 acagtgattt ttaatttgaa tccttctttg ctttgcggcg tggcatctaa tggtcggatc
   152521 aattgataat tgcctccgtt tgtttctgta aatccattat cgcgcaatgt atatcttttt
   152581 gcatcaggat gcaaacgata gctaattggt gcgcccagta ccgttgctgt tgtttcaaat
   152641 gccatgattt tcacctcgat aaaattagtt tctctttatt ataaaagttt atcctgattt
   152701 ttactaggtt tttcagttaa gttccctaaa aaatgaaggc gttctctcag acatctgttc
   152761 atataagaat gacttcacta gttatttttt gacagcacga actaattgat caaggaaata
   152821 ctggatgtcg tcttcggtat ttcccaaacc aaagctgata cggatggatt cttttagtat
   152881 cggtgtatct tctccataca ttgcttccaa tacgtgtgaa ggctcgatat ttcctgctgt
   152941 acaagctgaa ccagtagaaa tagcgacccc attaagatca agcttcatca gtagcaaatc
   153001 atttgcttcg ccaattaagc gaagattcaa cacatgaggg agtttattcg ttaaatcacc
   153061 atttacttga taagggattt cgtttgcttc taatgtatca agaatcaatt gctggaatcc
   153121 tttatatttg ttgttacgtg cttcacgttc ttcaggtgac agcagttcaa ctgcttttgc
   153181 cataccccag atccctgcta aattttctgt acctgcacgc cgcttttctt cctgctcccc
   153241 gcctaaaagc aatgcaggga tatttactcc gtctcgttta tataagaaac cgacgccttt
   153301 tggcccattg attttatgag cagagatact taataaatcg attcctaatt cttctggatg
   153361 gatcgtttgg tttccgtacg cttgtaccgc atctgtatgg aaataagctg gatgatccgc
   153421 taaaagttca ccgatttctt tgattggcat caaatttccg atttcattgt tcccgtacat
   153481 cacagagacc aagatcgtat cttctcgtaa cgcttttttg acctcttcga tcgacagatt
   153541 ccctttttca tctacaggca gatatgtaac gtcaaacccc tttgtctcta aatatttcat
   153601 ggtattaaga acagcaggat gttcgatagc tgtagtgatt agatgtctgc cttctttttg
   153661 acgcgagaac gctgtttcta agatagctgt attatcccct tctgttcccc cactgttaaa
   153721 gatgatctcg tgaggttttg cttgcagact gtctgcaatg atttgtcttg ctgtttccaa
   153781 tttttcatgc gcgtggcgac caaatccatg tatgcttgaa ggatttccaa acgtgtcttg
   153841 catgattgcc gtcatcgctt caattacttg tggatgcatg ggggtagttg ccgcatgatc
   153901 cagataaata gttttcactt cattcatccc ttccaaatta ttgctaggca cattctagca
   153961 taaaatagcg aacaaaccaa agaagagctc atttttgtct agaggacgtt tcatgggcat
   154021 agaaaaaagg agctgtgaca aaagtcttgt cgcagctcct tgaagctgct cacttctgtc
   154081 acaacttctt t
//
LOCUS       DAJSBL010000002       130572 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.002, whole genome shotgun sequence.
ACCESSION   DAJSBL010000002 DAJSBL010000000
VERSION     DAJSBL010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 130572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 130572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..130572
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.002"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            211..603
                     /locus_tag="ONT85_000139"
     CDS             211..603
                     /locus_tag="ONT85_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HCR2377144.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
     gene            631..1971
                     /gene="glnA"
                     /locus_tag="ONT85_000140"
     CDS             631..1971
                     /gene="glnA"
                     /locus_tag="ONT85_000140"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HCR2377145.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            3164..4774
                     /locus_tag="ONT85_000141"
     CDS             3164..4774
                     /locus_tag="ONT85_000141"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HCR2377146.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            5066..5935
                     /locus_tag="ONT85_000142"
     CDS             5066..5935
                     /locus_tag="ONT85_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HCR2377147.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            6100..7371
                     /locus_tag="ONT85_000143"
     CDS             6100..7371
                     /locus_tag="ONT85_000143"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HCR2377148.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            7368..8663
                     /gene="rho"
                     /locus_tag="ONT85_000144"
     CDS             7368..8663
                     /gene="rho"
                     /locus_tag="ONT85_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HCR2377149.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            8762..9025
                     /locus_tag="ONT85_000145"
     CDS             8762..9025
                     /locus_tag="ONT85_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HCR2377150.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            9470..9919
                     /locus_tag="ONT85_000146"
     CDS             9470..9919
                     /locus_tag="ONT85_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCR2377151.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            9976..11394
                     /locus_tag="ONT85_000147"
     CDS             9976..11394
                     /locus_tag="ONT85_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCR2377152.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            11394..12254
                     /locus_tag="ONT85_000148"
     CDS             11394..12254
                     /locus_tag="ONT85_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HCR2377153.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            12428..13201
                     /locus_tag="ONT85_000149"
     CDS             12428..13201
                     /locus_tag="ONT85_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR2377154.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            13348..13713
                     /locus_tag="ONT85_000150"
     CDS             13348..13713
                     /locus_tag="ONT85_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HCR2377155.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(13861..15069)
                     /locus_tag="ONT85_000151"
     CDS             complement(13861..15069)
                     /locus_tag="ONT85_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR2377156.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(15088..15366)
                     /locus_tag="ONT85_000152"
     CDS             complement(15088..15366)
                     /locus_tag="ONT85_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2377157.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            15520..16215
                     /locus_tag="ONT85_000153"
     CDS             15520..16215
                     /locus_tag="ONT85_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HCR2377158.1"
                     /translation="MIFLDESLDGGASLDSRVLKEYLDKNDFPIIRKGYHKYLTFEGM
                     EDSHTYILKEGTVKASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESE
                     YAEFYRINRVRFWQNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLY
                     ELYDLFGVRTEEGMLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIV
                     ILNMDLLEDNIIF"
     gene            complement(16657..17454)
                     /locus_tag="ONT85_000154"
     CDS             complement(16657..17454)
                     /locus_tag="ONT85_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HCR2377159.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(17451..18635)
                     /locus_tag="ONT85_000155"
     CDS             complement(17451..18635)
                     /locus_tag="ONT85_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HCR2377160.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(18660..19907)
                     /locus_tag="ONT85_000156"
     CDS             complement(18660..19907)
                     /locus_tag="ONT85_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HCR2377161.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            20122..21867
                     /gene="dnaX"
                     /locus_tag="ONT85_000157"
     CDS             20122..21867
                     /gene="dnaX"
                     /locus_tag="ONT85_000157"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HCR2377162.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            21887..22201
                     /locus_tag="ONT85_000158"
     CDS             21887..22201
                     /locus_tag="ONT85_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HCR2377163.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            22226..22822
                     /gene="recR"
                     /locus_tag="ONT85_000159"
     CDS             22226..22822
                     /gene="recR"
                     /locus_tag="ONT85_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HCR2377164.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            22886..23530
                     /locus_tag="ONT85_000160"
     CDS             22886..23530
                     /locus_tag="ONT85_000160"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HCR2377165.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            23545..23874
                     /locus_tag="ONT85_000161"
     CDS             23545..23874
                     /locus_tag="ONT85_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HCR2377166.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            23888..24826
                     /gene="holB"
                     /locus_tag="ONT85_000162"
     CDS             23888..24826
                     /gene="holB"
                     /locus_tag="ONT85_000162"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HCR2377167.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            24862..25686
                     /locus_tag="ONT85_000163"
     CDS             24862..25686
                     /locus_tag="ONT85_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HCR2377168.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            25679..26026
                     /locus_tag="ONT85_000164"
     CDS             25679..26026
                     /locus_tag="ONT85_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HCR2377169.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            26095..26967
                     /gene="rsmI"
                     /locus_tag="ONT85_000165"
     CDS             26095..26967
                     /gene="rsmI"
                     /locus_tag="ONT85_000165"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HCR2377170.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            27076..28197
                     /gene="dinB"
                     /locus_tag="ONT85_000166"
     CDS             27076..28197
                     /gene="dinB"
                     /locus_tag="ONT85_000166"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HCR2377171.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(28251..28853)
                     /gene="nrdG"
                     /locus_tag="ONT85_000167"
     CDS             complement(28251..28853)
                     /gene="nrdG"
                     /locus_tag="ONT85_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HCR2377172.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYAFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(29168..31357)
                     /gene="nrdD"
                     /locus_tag="ONT85_000168"
     CDS             complement(29168..31357)
                     /gene="nrdD"
                     /locus_tag="ONT85_000168"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HCR2377173.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKNQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGSFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            31656..32450
                     /locus_tag="ONT85_000169"
     CDS             31656..32450
                     /locus_tag="ONT85_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR2377174.1"
                     /translation="MKNERGGEELEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGE
                     FVGIMGPSGAGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQ
                     EFNLIDTLNAKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQ
                     RIAAARALVVQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTAS
                     YCDRILFIKDGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            32440..34482
                     /locus_tag="ONT85_000170"
     CDS             32440..34482
                     /locus_tag="ONT85_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR2377175.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
     gene            34763..34912
                     /locus_tag="ONT85_000171"
     CDS             34763..34912
                     /locus_tag="ONT85_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HCR2377176.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            34923..36437
                     /gene="dltA"
                     /locus_tag="ONT85_000172"
     CDS             34923..36437
                     /gene="dltA"
                     /locus_tag="ONT85_000172"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HCR2377177.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            36434..37639
                     /gene="dltB"
                     /locus_tag="ONT85_000173"
     CDS             36434..37639
                     /gene="dltB"
                     /locus_tag="ONT85_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HCR2377178.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            37684..37917
                     /gene="dltC"
                     /locus_tag="ONT85_000174"
     CDS             37684..37917
                     /gene="dltC"
                     /locus_tag="ONT85_000174"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HCR2377179.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWSTPNKIIQQATALKG"
     gene            37920..39188
                     /gene="dltD"
                     /locus_tag="ONT85_000175"
     CDS             37920..39188
                     /gene="dltD"
                     /locus_tag="ONT85_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HCR2377180.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            39329..40411
                     /locus_tag="ONT85_000176"
     CDS             39329..40411
                     /locus_tag="ONT85_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HCR2377181.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            40411..41214
                     /locus_tag="ONT85_000177"
     CDS             40411..41214
                     /locus_tag="ONT85_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HCR2377182.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            41284..42024
                     /locus_tag="ONT85_000178"
     CDS             41284..42024
                     /locus_tag="ONT85_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HCR2377183.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            42035..43039
                     /locus_tag="ONT85_000179"
     CDS             42035..43039
                     /locus_tag="ONT85_000179"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HCR2377184.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(43084..44928)
                     /locus_tag="ONT85_000180"
     CDS             complement(43084..44928)
                     /locus_tag="ONT85_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2377185.1"
                     /translation="MSFFNNIYNKKESFYLRIKWSIIIILSLLFLTGIAYFFYFIFSP
                     ADSNQMKEIKDSWVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLEKPLDEANLL
                     IKGNHQWITVYLGEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTLTVSSPYQNY
                     AGLPPKVYLGTTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMYKQKRLEYSL
                     ILLSCFALALGFEAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYYWSRMFYYKK
                     WYGIFCTFQLLIFIGVLLYATFTPAELPELMPIANIASVISTLVTSLACIGEAYKNNR
                     FYVACTPWIVLVAIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISYNLIDHILNT
                     DKDRRQVDFLKIKNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLMHTDQTKAKN
                     YVEKILEEAKNFEMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLPEELPINDDD
                     LAQLLIHALEHSFRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYDTNLFSRGIT
                     EELDDQERFDLMMMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
     gene            45140..45658
                     /locus_tag="ONT85_000181"
     CDS             45140..45658
                     /locus_tag="ONT85_000181"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HCR2377186.1"
                     /translation="MPDYKKITFISSITLIIFLILILLVVLVPRIFGLTPYIIEDKEM
                     SPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTK
                     TYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVE
                     LKKKRRRTQKFL"
     gene            45786..46469
                     /locus_tag="ONT85_000182"
     CDS             45786..46469
                     /locus_tag="ONT85_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HCR2377187.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            46778..46851
                     /locus_tag="ONT85_000183"
     tRNA            46778..46851
                     /locus_tag="ONT85_000183"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:46812..46814,aa:Arg,seq:tct)
     gene            47010..47369
                     /locus_tag="ONT85_000184"
     CDS             47010..47369
                     /locus_tag="ONT85_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 family protein"
                     /protein_id="HCR2377188.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            47492..49123
                     /locus_tag="ONT85_000185"
     CDS             47492..49123
                     /locus_tag="ONT85_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HCR2377189.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(49185..49826)
                     /locus_tag="ONT85_000186"
     CDS             complement(49185..49826)
                     /locus_tag="ONT85_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HCR2377190.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     misc_feature    50012..50088
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            50108..50416
                     /gene="rplU"
                     /locus_tag="ONT85_000187"
     CDS             50108..50416
                     /gene="rplU"
                     /locus_tag="ONT85_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HCR2377191.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            50443..50787
                     /locus_tag="ONT85_000188"
     CDS             50443..50787
                     /locus_tag="ONT85_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HCR2377192.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            50800..51093
                     /gene="rpmA"
                     /locus_tag="ONT85_000189"
     CDS             50800..51093
                     /gene="rpmA"
                     /locus_tag="ONT85_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HCR2377193.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            51527..54334
                     /locus_tag="ONT85_000190"
     CDS             51527..54334
                     /locus_tag="ONT85_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HCR2377194.1"
                     /translation="MYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNSSKDLNQLVR
                     EGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDTKDIFAKIIG
                     ANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAHHVIDEETEL
                     IVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLDEIHRLPPEG
                     QEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRIPINIKLPAF
                     EKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQLKSNIQLVCA
                     RGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKITVAPNDTIMK
                     IQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFYKDHGFSFNA
                     DSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKKINLGEERHT
                     NDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLRANQASGRIG
                     VVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNVQEVDEGSGV
                     ILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDLDMLHESLKN
                     FRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNKRQETELEVL
                     ALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVILDQLLMESE
                     LEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTTGIGDEEYGF
                     KINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLEDQIKVKVPIE
                     EMYYLLRLVHNQLDKKEYTVP"
     gene            54525..55016
                     /locus_tag="ONT85_000191"
     CDS             54525..55016
                     /locus_tag="ONT85_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCR2377195.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            55119..56108
                     /gene="manX"
                     /locus_tag="ONT85_000192"
     CDS             55119..56108
                     /gene="manX"
                     /locus_tag="ONT85_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HCR2377196.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            56141..56944
                     /locus_tag="ONT85_000193"
     CDS             56141..56944
                     /locus_tag="ONT85_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HCR2377197.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            56965..57879
                     /locus_tag="ONT85_000194"
     CDS             56965..57879
                     /locus_tag="ONT85_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HCR2377198.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            57962..58336
                     /locus_tag="ONT85_000195"
     CDS             57962..58336
                     /locus_tag="ONT85_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HCR2377199.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGDNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            58475..58720
                     /locus_tag="ONT85_000196"
     CDS             58475..58720
                     /locus_tag="ONT85_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HCR2377200.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            58892..59956
                     /locus_tag="ONT85_000197"
     CDS             58892..59956
                     /locus_tag="ONT85_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HCR2377201.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            60202..60627
                     /locus_tag="ONT85_000198"
     CDS             60202..60627
                     /locus_tag="ONT85_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HCR2377202.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            60620..61069
                     /gene="nusB"
                     /locus_tag="ONT85_000199"
     CDS             60620..61069
                     /gene="nusB"
                     /locus_tag="ONT85_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HCR2377203.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            61166..62020
                     /locus_tag="ONT85_000200"
     CDS             61166..62020
                     /locus_tag="ONT85_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HCR2377204.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            62021..63358
                     /locus_tag="ONT85_000201"
     CDS             62021..63358
                     /locus_tag="ONT85_000201"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HCR2377205.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            63363..63587
                     /locus_tag="ONT85_000202"
     CDS             63363..63587
                     /locus_tag="ONT85_000202"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HCR2377206.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            63589..64479
                     /locus_tag="ONT85_000203"
     CDS             63589..64479
                     /locus_tag="ONT85_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HCR2377207.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            64488..65306
                     /locus_tag="ONT85_000204"
     CDS             64488..65306
                     /locus_tag="ONT85_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HCR2377208.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            65413..65862
                     /locus_tag="ONT85_000205"
     CDS             65413..65862
                     /locus_tag="ONT85_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HCR2377209.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            65880..67562
                     /gene="recN"
                     /locus_tag="ONT85_000206"
     CDS             65880..67562
                     /gene="recN"
                     /locus_tag="ONT85_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HCR2377210.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(67617..67733)
                     /locus_tag="ONT85_000207"
     CDS             complement(67617..67733)
                     /locus_tag="ONT85_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HCR2377211.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(67806..68762)
                     /locus_tag="ONT85_000208"
     CDS             complement(67806..68762)
                     /locus_tag="ONT85_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HCR2377212.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            68933..69301
                     /locus_tag="ONT85_000209"
     CDS             68933..69301
                     /locus_tag="ONT85_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HCR2377213.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            69471..69902
                     /gene="mraZ"
                     /locus_tag="ONT85_000210"
     CDS             69471..69902
                     /gene="mraZ"
                     /locus_tag="ONT85_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HCR2377214.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            69923..70882
                     /gene="rsmH"
                     /locus_tag="ONT85_000211"
     CDS             69923..70882
                     /gene="rsmH"
                     /locus_tag="ONT85_000211"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HCR2377215.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            70887..71288
                     /gene="ftsL"
                     /locus_tag="ONT85_000212"
     CDS             70887..71288
                     /gene="ftsL"
                     /locus_tag="ONT85_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HCR2377216.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            71289..73481
                     /locus_tag="ONT85_000213"
     CDS             71289..73481
                     /locus_tag="ONT85_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HCR2377217.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKNGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            73507..74469
                     /locus_tag="ONT85_000214"
     CDS             73507..74469
                     /locus_tag="ONT85_000214"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HCR2377218.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            74483..75844
                     /locus_tag="ONT85_000215"
     CDS             74483..75844
                     /locus_tag="ONT85_000215"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HCR2377219.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            75870..76958
                     /gene="murG"
                     /locus_tag="ONT85_000216"
     CDS             75870..76958
                     /gene="murG"
                     /locus_tag="ONT85_000216"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HCR2377220.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            76973..78193
                     /locus_tag="ONT85_000217"
     CDS             76973..78193
                     /locus_tag="ONT85_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HCR2377221.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            78349..79674
                     /gene="ftsA"
                     /locus_tag="ONT85_000218"
     CDS             78349..79674
                     /gene="ftsA"
                     /locus_tag="ONT85_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HCR2377222.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            79697..80938
                     /gene="ftsZ"
                     /locus_tag="ONT85_000219"
     CDS             79697..80938
                     /gene="ftsZ"
                     /locus_tag="ONT85_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HCR2377223.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            80955..81632
                     /locus_tag="ONT85_000220"
     CDS             80955..81632
                     /locus_tag="ONT85_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HCR2377224.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            81646..82245
                     /locus_tag="ONT85_000221"
     CDS             81646..82245
                     /locus_tag="ONT85_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HCR2377225.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            82260..82547
                     /locus_tag="ONT85_000222"
     CDS             82260..82547
                     /locus_tag="ONT85_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HCR2377226.1"
                     /translation="MYTIALLLHLLQRAAYFYSIILMVYALLSWFPGGYQSGLGRLLA
                     KICEPYISLFDRLPLRIGPLDFTILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            82596..83378
                     /locus_tag="ONT85_000223"
     CDS             82596..83378
                     /locus_tag="ONT85_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HCR2377227.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            83399..84106
                     /locus_tag="ONT85_000224"
     CDS             83399..84106
                     /locus_tag="ONT85_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HCR2377228.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            84368..87166
                     /gene="ileS"
                     /locus_tag="ONT85_000225"
     CDS             84368..87166
                     /gene="ileS"
                     /locus_tag="ONT85_000225"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HCR2377229.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(87215..88741)
                     /locus_tag="ONT85_000226"
     CDS             complement(87215..88741)
                     /locus_tag="ONT85_000226"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HCR2377230.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(88756..89403)
                     /locus_tag="ONT85_000227"
     CDS             complement(88756..89403)
                     /locus_tag="ONT85_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HCR2377231.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(89587..89916)
                     /locus_tag="ONT85_000228"
     CDS             complement(89587..89916)
                     /locus_tag="ONT85_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HCR2377232.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            90093..90821
                     /gene="sfsA"
                     /locus_tag="ONT85_000229"
     CDS             90093..90821
                     /gene="sfsA"
                     /locus_tag="ONT85_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HCR2377233.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            90837..91850
                     /locus_tag="ONT85_000230"
     CDS             90837..91850
                     /locus_tag="ONT85_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR2377234.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            91850..93127
                     /locus_tag="ONT85_000231"
     CDS             91850..93127
                     /locus_tag="ONT85_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCR2377235.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            93190..95892
                     /locus_tag="ONT85_000232"
     CDS             93190..95892
                     /locus_tag="ONT85_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HCR2377236.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            96044..96361
                     /locus_tag="ONT85_000233"
     CDS             96044..96361
                     /locus_tag="ONT85_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCR2377237.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            96391..96711
                     /locus_tag="ONT85_000234"
     CDS             96391..96711
                     /locus_tag="ONT85_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HCR2377238.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            96819..98279
                     /locus_tag="ONT85_000235"
     CDS             96819..98279
                     /locus_tag="ONT85_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCR2377239.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGTLPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGPNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            98347..98568
                     /locus_tag="ONT85_000236"
     CDS             98347..98568
                     /locus_tag="ONT85_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2377240.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            98599..98781
                     /locus_tag="ONT85_000237"
     CDS             98599..98781
                     /locus_tag="ONT85_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HCR2377241.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            98781..99194
                     /locus_tag="ONT85_000238"
     CDS             98781..99194
                     /locus_tag="ONT85_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HCR2377242.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            99317..100498
                     /locus_tag="ONT85_000239"
     CDS             99317..100498
                     /locus_tag="ONT85_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR2377243.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            complement(100569..100733)
                     /locus_tag="ONT85_000240"
     CDS             complement(100569..100733)
                     /locus_tag="ONT85_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2377244.1"
                     /translation="MNDKPKEKVYAERLDSSKKNREKITKEAIQDHKKENPKTKENKK
                     FKFTKKEDLL"
     gene            100887..100960
                     /locus_tag="ONT85_000241"
     tRNA            100887..100960
                     /locus_tag="ONT85_000241"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:100921..100923,aa:Arg,seq:cct)
     gene            complement(100997..101197)
                     /locus_tag="ONT85_000242"
     CDS             complement(100997..101197)
                     /locus_tag="ONT85_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR2377245.1"
                     /translation="MKINYTVDLVSFSLVINFSLFSLRLFIDGLRHTHASILLYQGVN
                     ILSVSKRLGHSSLETTMSTYTK"
     gene            complement(101502..102734)
                     /locus_tag="ONT85_000243"
     CDS             complement(101502..102734)
                     /locus_tag="ONT85_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HCR2377246.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            102988..103557
                     /locus_tag="ONT85_000244"
     CDS             102988..103557
                     /locus_tag="ONT85_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCR2377247.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(103735..104175)
                     /locus_tag="ONT85_000245"
     CDS             complement(103735..104175)
                     /locus_tag="ONT85_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HCR2377248.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            104333..105097
                     /gene="map"
                     /locus_tag="ONT85_000246"
     CDS             104333..105097
                     /gene="map"
                     /locus_tag="ONT85_000246"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HCR2377249.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            105129..106052
                     /locus_tag="ONT85_000247"
     CDS             105129..106052
                     /locus_tag="ONT85_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HCR2377250.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            106128..107267
                     /locus_tag="ONT85_000248"
     CDS             106128..107267
                     /locus_tag="ONT85_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HCR2377251.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIVLIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            107260..108060
                     /locus_tag="ONT85_000249"
     CDS             107260..108060
                     /locus_tag="ONT85_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HCR2377252.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            108060..108887
                     /locus_tag="ONT85_000250"
     CDS             108060..108887
                     /locus_tag="ONT85_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HCR2377253.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            108865..109599
                     /locus_tag="ONT85_000251"
     CDS             108865..109599
                     /locus_tag="ONT85_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HCR2377254.1"
                     /translation="MLRIGRCEFVRSVCIATFNGGRYLREQLDSILSQLSAEDEVVIS
                     DDGSVDDTWDILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWL
                     ETKVKKITDYFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYI
                     GAGMAFRSSLKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIET
                     HSKLRQKISWRVHLITALIKRKWFKR"
     gene            109699..110565
                     /gene="rfbA"
                     /locus_tag="ONT85_000252"
     CDS             109699..110565
                     /gene="rfbA"
                     /locus_tag="ONT85_000252"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HCR2377255.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            110579..111151
                     /gene="rfbC"
                     /locus_tag="ONT85_000253"
     CDS             110579..111151
                     /gene="rfbC"
                     /locus_tag="ONT85_000253"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HCR2377256.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            111173..112201
                     /gene="rfbB"
                     /locus_tag="ONT85_000254"
     CDS             111173..112201
                     /gene="rfbB"
                     /locus_tag="ONT85_000254"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HCR2377257.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTSILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWQPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            112299..113150
                     /gene="rfbD"
                     /locus_tag="ONT85_000255"
     CDS             112299..113150
                     /gene="rfbD"
                     /locus_tag="ONT85_000255"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HCR2377258.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLPYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEALGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            113185..115218
                     /locus_tag="ONT85_000256"
     CDS             113185..115218
                     /locus_tag="ONT85_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2377259.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFSLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMIAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFVALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            115262..116542
                     /locus_tag="ONT85_000257"
     CDS             115262..116542
                     /locus_tag="ONT85_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HCR2377260.1"
                     /translation="MKIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGVGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LVILFCLVIKWIEWTKWDRGIYLIVFTVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNTIGSSLMTLALLISGFASSFERWYVRIIGGIAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTDREDIWHKFY
                     QTLGEKSEQVLLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVAEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            116752..117558
                     /locus_tag="ONT85_000258"
     CDS             116752..117558
                     /locus_tag="ONT85_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR2377261.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            117570..118790
                     /locus_tag="ONT85_000259"
     CDS             117570..118790
                     /locus_tag="ONT85_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR2377262.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            118780..120366
                     /locus_tag="ONT85_000260"
     CDS             118780..120366
                     /locus_tag="ONT85_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HCR2377263.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAEPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSETT
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKELPEFVLTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSREEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            120405..122543
                     /locus_tag="ONT85_000261"
     CDS             120405..122543
                     /locus_tag="ONT85_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HCR2377264.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRKENGHISLATNSALEIAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDVIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYSNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTVMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPAYIAHDPFYNDNLTKDGIGDFSLRPD"
     gene            complement(122911..123894)
                     /locus_tag="ONT85_000262"
     CDS             complement(122911..123894)
                     /locus_tag="ONT85_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HCR2377265.1"
                     /translation="MNKQELHDFIGFRQPNICQIVAYKDGVEVYTDEWNGYKKDDTCH
                     IMSATKSIVSLLIGIALEQGLIKSIDDKVLDYFPEYKVKRGEKTIYAITIKHLLTMKA
                     PYKCKGDPWTKVCSSEDWTKTSLDLLGGRKGITGEFNYQTVCLHILTGLLYQTSKLTT
                     VDFANKYLFDPIGIPNHVNYYAETAEEHKQFTMDKSPKTNVWFCDPLNIGTAGYGLCL
                     SASDLAKIGVLCLNKGMYKGKQIVPPMWINEMFKPRTSALEDLQNMRYGYLWWVLDSE
                     NEIYSAIGNSGNVLYIDAKNNLVISITSYFKPMIIDRVAFVEEYLKPFILK"
     gene            124272..125663
                     /locus_tag="ONT85_000263"
     CDS             124272..125663
                     /locus_tag="ONT85_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HCR2377266.1"
                     /translation="MNKNGEWSAARRIFIILMDVIVYNAAIYSSFLLKFRGEIPLRNL
                     ETFQHSAIIISVIFIALNILLGAYVFYNRMISDIVFVTVIGQVLMTLGIMVVTFAGRW
                     FAFPRLVILLSFVLGTLFLSIYRILVYKLYLRVSRDKKVVIIGLEKDVAPAIQNFGSK
                     KNNKHKVEAVILDNYYANIKNMIKDIDIVYLASHIEETEKIKIYQLVTKNEKKLFLNT
                     TFENLTLINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLVLLVIASPFMLFT
                     AILVKVTSPGPIIYKQVRITKNQKEFSIYKFRSMTATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERSFFVDQFNAENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLMLDLKILLQTIKILFDKMSSQGLEEDENVIS
                     LAEIAFPNEKIFR"
     gene            125679..126719
                     /locus_tag="ONT85_000264"
     CDS             125679..126719
                     /locus_tag="ONT85_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HCR2377267.1"
                     /translation="MNNPKFSIIVPVYNAEATIGRTIDILKELDYEEYEVVLVNDGST
                     DQTQEIIQELIENDERFELITTTNQGPGLARNEGIRQSKGEYLLFFDADDTPVKTILS
                     DYDHLLEKSPKADLVVSSFVFRTMDEGRIVSEKENLVTECHYLTHEEFIKDMYQLMNE
                     QLMYVVWNKCYRSDILKNNQILFKTYNSCEDRIFNLHYYEYCHQVIMNPKVEYIYEFE
                     GGKGITNQYRPNKFETFKEFYSLANTVTDDINKDGMAALLLKGTTSVIFSIYSTSART
                     SKEKKDEAKQILSDPVIREAKQIAKTDSSAKKITKLLYNMPAPLFLSAVKVGSFVEVK
                     MPGLMAALKRVY"
     gene            126738..127823
                     /locus_tag="ONT85_000265"
     CDS             126738..127823
                     /locus_tag="ONT85_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HCR2377268.1"
                     /translation="MKRILHFQGRMGLGGAESFMMNLYRKIDRTNYQFDFLIYEDYAD
                     VQDYHSEIERLGGRIFVVPNPKKNIFKYLIEVNKLLKKESFSIVHNQVYFGGGINLWL
                     AKKNGIRQRIAHSHATEDGKSQNIVMNVLRKFLTKLLLQNATDYLAVSQEAGESLFQN
                     HPFEIVHNGIDLELYSKNSEAKVNKRKELDISMSTFVVGNIGRLEKQKNQSYLLEIFL
                     EILKMNSDSYLILVGEGSLRNKLKAKAVELDIEKRVKFLGERNDIPELLSVMDVFVMP
                     SLYEGLPMVGLEAQAAKLKLVLADTISKDTKLTNNVDFVSLDETPQEWAKKVIKEPYA
                     NKITQELKSYDVSYTTEQMESIYSRDH"
     gene            127856..128818
                     /locus_tag="ONT85_000266"
     CDS             127856..128818
                     /locus_tag="ONT85_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HCR2377269.1"
                     /translation="MKICYLILAYNDIGNLKRLVNRLNENAVFIIHIDKKTDIEPFIE
                     AFKSYDNVEFLCNKNRVKIYWGGFSIVQAEINLVKRALQNEKYLKYVLLSGADYPIKD
                     NEYIYNYFKKNSSVEFIRGIDLDQIKHKEFYYKHIDVYQKHDYPRINRNNTTAFKIFR
                     AIINRCLRMIKLPPKIRHHKFDLYHGSQWWALSKECLTELIQMYEQNQQDYLNFKIGM
                     FAPDEKFFHTLFFNSSFKNKNVIGGPDLPLELKNIEETTLQTSKLANIHIIDPSMNKW
                     FDERDIDQIKESEKLFVRKVKSDYSDKLLNRIDKEILKVGNIDE"
     gene            128811..130238
                     /locus_tag="ONT85_000267"
     CDS             128811..130238
                     /locus_tag="ONT85_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HCR2377270.1"
                     /translation="MNRIVPVLEKSLLSRDAFRVINLIICLLYFMPSTFNVTNIPMKI
                     AFAWGGIIVLYEVFIKKNFFKMRFSWLLIGAMISFILTIIINRENFLIPSIYNFGYLL
                     ITLIVIFPADFSQSEEEKKKKMVYFNNVFIIIIFLAALISIYQFIFLISYHVPTGTPG
                     LLARQGFIEHRLFGVYTSPNVGAMFGYTSIILSLISVLISKITKTIRVFYMMNFIVQI
                     FYITLSSSRGAQVVICSFLVLFLLLFANSKFRKSIREVLPVKIKWMVSILLVLLVYFS
                     FGTNYAKDFLATIPVSIDRKVEVIESNESDKTPLPEKEVILQHSSDNSEISAGRFTIW
                     KAALKVIRPTLLFGYGETDFYGNSDAKFLKKVDLDTTDVNELKRAHGNMHNGFLQVLV
                     SSGIIAFICIYAFYVLNIFSLLKTFFRSKVNYAFLGIVLIFILSIFIDEMVEAHVLFN
                     KRDVISIVFWYYLGSISYLYLKKEN"
     gene            130240..>130572
                     /locus_tag="ONT85_000268"
     CDS             130240..>130572
                     /locus_tag="ONT85_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2377271.1"
                     /translation="MKTAFIAWTPLHLINILNVYQNFYRESKGDLFIYSEFENARRYS
                     ERIKELGLFNDVVFVNHRKIGSSFKRKINLLTNHNQFFSDDLSVYDEIFIQGENYFTK
                     ILFSNVYKK"
BASE COUNT        42546 a        22091 c        27325 g        38610 t
ORIGIN      
        1 ataaaaacac taataaagga tagtacagta taataaaaaa taaaaggtat ctggtacgaa
       61 aatcaactag ataccttttt tgtgttctta tttttttgtt attttataaa aaatagagat
      121 ctcatgttag aaaacctaac atatgcagtt gacaatattt tttttaattg gtacacttta
      181 gccaacttat aaaacgaaag gggacgtctg atgggagaaa aggaattgcg ccgatctatg
      241 tccgtatttc caattggtac agttatgaag ctgactgatt tgtctgctcg gcaaattcgc
      301 tattatgaag aacaagattt agttcatccg gaacgaagcg aaggaaaccg acgtatgtat
      361 tcgctgaatg atattgatat gttgcttgag attaaagact atctttcaga gggattgaat
      421 atggctggga tcaaacgtgt atacgaaatg gcacaggcga aaaaacaaga agcaaaaaac
      481 aagaagccgt tgacagatca tgacgttcga caaatttttc gtgatgagat tttggcgcaa
      541 ggtgggttaa gcaaaacagg tcattatcag tcgcaaggat tgcaaagaca attctttgac
      601 tgaataaatt aaaaaaggaa gagggatacc atggtaaaga aacagataac aggtgaagaa
      661 atcaaacgaa tcattgagga agaaaacgta cgatttttac gattgatgtt cacagacatc
      721 ttaggtacga tcaaaaatgt agaagttcca gtgagccaga tcgacaaagt attagaaaat
      781 aaaatgatgt ttgacggttc ttcgattgaa ggatttgttc gaattgaaga aagcgatatg
      841 tatttgtatc cagatctctc cacttggatg atcttcccgt gggaaagtgc tcatggaaaa
      901 gttgctcgtt taatttgtga tatctataat ccggatggga caccatttgc cggcgatcct
      961 cgcgggaatt tgaaacgagc attgaaagat atgcgcgacc ttggttttac ctctttcaat
     1021 ttaggacctg aaccagaatt cttcttattt aaattagacg aagacggtgg gatcacaacg
     1081 accttgaatg ataaaggtgg ttattttgat tttgcaccaa cagatttagg tgaaaattgc
     1141 cgccgtgaca tcgtgttgga attagaaagt ctaggctttg aagtggaagc ttcacatcat
     1201 gaagtagcgc caggacagca tgaaattgac tttaaatatg cagatgtggt agatgcttgt
     1261 gataatatcc aaaccttcaa gctcgttgtc aaaacgattg cacgcaagca tggacttcac
     1321 gcgactttca tgccgaaacc gttatttggg atcaatggtt ctggtatgca ttgcaatatg
     1381 tctttgttca atgatgaagg aaatgttttc tatgataaag acggtgaatt ggaactaagt
     1441 gaaacagctt atcatttctt aggtggtttg ctgaaacatg ctcgtgccta cacagccgtt
     1501 tgcaatccaa cagtcaattc ttataaacgt ctcgttccag gttatgaagc gcctgtttat
     1561 gtcgcatgga gtggccgtaa tcgttcgcca ttagtacgtg taccagaatc tcgcggttta
     1621 tccacacgtt tggaattgcg gtcagttgat ccatctgcaa acccatactt ggcaatggca
     1681 gtattgttgc aagcaggact agacggtatc cgtaatcaga tcacaccgcc agcagctgtt
     1741 gaccgtaaca tttatgtaat ggatgaggaa gaaagagaag ctgcacatat ccaggactta
     1801 ccttcaacta tccataatgc tatcaaagaa ttgcgtaagg ataatgtaat gattgacgca
     1861 ttaggacaac atatcttctc aaattttgtt gaagcaaaac gtctcgaatg ggcggcattc
     1921 agacaaactg tttccgagtg ggaaagagaa caatatctag aactttatta aaacaggaat
     1981 accgtgaatt agaggctgtg aaatcagcgt tttgacctga gtattagaga aactaacgga
     2041 aaaatagttc ctcatatttt gccgtttttt gagctaatac cgagggtcag ctgattgaac
     2101 accgtttagt ggagaggttg tgaaaaagct gccctgcaac aagtaataag aatagccaag
     2161 caaaaacagc ttttcttgtt tttgaattgg ctgaacttat taccgcagat gcaagctttt
     2221 gaacaccgtt taccaagagg ttttgaaaaa gacgtttagt ttcgagtatt aagaaaaatt
     2281 ttgtgaaaat ggctttctac atttttgcat aaatttggct taataccgag aaactggctt
     2341 ttgaataccg tttatccaga ggttgtgagc ctaccattta actccgaatc acaagaaata
     2401 atttcaaaaa cagcttctca tgtttgatgg atattgtgac ttgtgaccga ggagttggca
     2461 ggagaacacc gtttatttag aaataaggag gcacgacttt tgtcgcatct ccttattttt
     2521 tacttatgtg attcgttacc ttcaatgaat ccctacaagg cacttttgtt gaaaaaaagg
     2581 tcatctttga aaaaattagt ggaattgttt tgtttttgat attatttaat agaaaagata
     2641 tgccaaaaat ccgagaactg tggactacgt tttttacgta aaggaggtaa ataagggtga
     2701 aagaaaaaga ttgtttctac gataagctat ataaactgtt gctgcaagta ggaaaaagta
     2761 gttctttgtt aggtgggata ttatctatca taatgtttgt tttatttttt tatctattat
     2821 ttcacttaaa atttttcagt aacgattttc tgatttgctt aggtttaacg ttagctatgt
     2881 ttatatcaag cttattatgt gggttaaatt ttttttgcac tcataaagga cttaagaaat
     2941 aatttctcaa aggatatcga aaaagaaaaa attgtaagcg gatagtattc atttgagtac
     3001 ttgactttcc cttagtcagc ttgtattata caccttgggc acccttttag agggtcagaa
     3061 gtttacagaa atttcggctc cctattctta ttgaagaata gggagctttg tttattgtct
     3121 gagacttttc ctgcacataa attaaaaagg agtttttgaa cttatgacaa aatatatttt
     3181 tgtgaccggc ggtgtcgttt catcaatcgg aaaagggatt gttgcagctt ctttaggacg
     3241 cttattgaaa aaccgcggct taaaagtaac tatccaaaaa tttgatccat acatcaacgt
     3301 ggatccgggg acgatgagcc catatcagca cggagaagta ttcgtaacag atgatggtgc
     3361 tgaaacagat ttggatctgg gccactacga acgttttatt gacatcaatt tgaacaaata
     3421 ctccaacgtg acgacaggaa aaatctattc tgaagttttg cgtaaagaac gtaagggcga
     3481 atacttggga gcaactgttc aagttattcc tcatattaca aatgagatca aagaaaaaat
     3541 catgcgagca gctaaaatga ctgattcaga tgtgatcatt acagaagtag ggggaacagt
     3601 aggggatata gaatctttgc cattccttga agcacttcgt caaatgaaag cagatgtcgg
     3661 tacagataat gttatgtata tccatacaac attgattcct tacttgaaag ctgctggaga
     3721 aatgaaaact aaaccaacgc agcacagtgt aaaagagcta cgtggtttag gtatccaacc
     3781 gaatattttg gtggtacgta cagagcaacc tgtttctcaa agcgtgaaaa acaaattggc
     3841 acaattttgt gatgtcgaac cagaagcagt tatcgagtcg ccagatgtag atacacttta
     3901 ctccatccca ttagttttac aagcacaagg aatggatcaa attgtttgcg accatttaaa
     3961 actagataca ccagaagcag acatgacaga atggaaagaa ttagaagaac gtgtcttgaa
     4021 cttgaagaaa aaagtacgga ttgcgcttgt cggtaaatat gtcgagttac cagatgcata
     4081 tatctcagta gttgaagcat tgaaacattc tggtttcgcc tataattcag acatcgagat
     4141 cgattggatc aaagcacaag aattaacgag agaaaatgta gaagaacgtt tgaaagacgc
     4201 agatggaatc ttggttcctg gtggatttgg tgatcgcgga gtagaaggga aaatcgaagc
     4261 gattcgttat gctcgcgaaa atgacgttcc gtttttaggt atttgtcttg gtatgcagat
     4321 ggcttgtgtc gaatttgcac gtaacgtcgt tggattagaa gatgcgtcat cagctgaaac
     4381 gaatccagat acagcaaaca atattattga tttaatggct gatcaggaaa acatcgagaa
     4441 tttgggtgga actttacgcc ttggattata cccatgtaaa atcaaaaaag gaacgaagac
     4501 ggctgaagca tatgatggag ccgatgttgt gcaagaacgt caccgtcacc gctatgagtt
     4561 caataacaaa tatcgtcaat tgtttgaaga acaagggcta gtcttttcag gtgtctcacc
     4621 agataaccgt ttagtagaaa tcgtagagct gccagagaaa aaattctttg tgggatgcca
     4681 attccaccca gaattgattt ctcgtccaaa tcgcccgcaa aaattaatca aagcatttgt
     4741 cggagcatca ttagcagatc acgaaacaaa ataatttttg aacttgatag aaagagaaaa
     4801 gtagcaagag aaggggataa gcaattttct tgtcactttt ctctttttct gatttgttat
     4861 tatttgtagg aaaaaagata gaaagcaaaa gacttttttc gattaaagcg ctattggata
     4921 ttttattttt tgcaaaaaac ttcttgaaaa ttttcatttt cattgatttt agtagaaaac
     4981 taccgaaatg tttggtaaaa tagggtcgtt ggttaagaat tgtcttaaca aatttagttt
     5041 tacaaaactt aggaggaatt ttattatgcc agtagtatca ggagctgaat ttttaaaagc
     5101 tgctcgtaaa ggcggttacg ctgtcggcgg attcaacaca aacaacttgg aatggactca
     5161 agcaatcctt gaagcggctg aagcaaaaca agcaccagta cttatccaaa catcaatggg
     5221 tgcagctaaa tacatgggtg gttacaaaat ctgtaaagat ttaatcactg atttagttga
     5281 agcaatgaac atcactgttc cagttgctat ccacttagac catggtgatt acgaagctgc
     5341 tttggaatgt atcgaattag gctatacttc agtaatgttc gatggttcac atctaccatt
     5401 tgaagaaaac ttgaaattag ctaaagacgt tgttgaaaaa gcacatgcaa aaggtatctc
     5461 agttgaatgt gaagttggtt caatcggcgg agaagaagac ggaatcatcg gtaacggcga
     5521 attagctgat attgaagaat gcaaacaaat ggttgctaca ggtatcgact acttagcttg
     5581 cggtatcggt aacatccatg gtcaatatcc agaaaactgg aaaggattag cttttgacca
     5641 cttacaagca attgctgatg ctgtaggtcc agacatgcct ttagtattac atggcggatc
     5701 aggtattcct caagatcaaa tcgaaaaagc tatctcaatg ggtatctcaa aagtaaatgt
     5761 taacactgaa ttccaattat catttgctgc agctactcgt aaatatatcg aagctggtaa
     5821 agataaagaa ggtaaaggat tcgaccctcg taaattatta gctccaggta aagcagcaat
     5881 cgttgaagat gctaaatctc atatcgactg gttcggttca gctaacaaag cgtaagcatc
     5941 cacataacag atacgatata taggaatgga gaacgacttt tgaagtcgct ctccattctt
     6001 ttttacttaa aacggcagac catgctcgtt tctttttttt atctatgata aaatacaagc
     6061 atatccatga aaaaagatta ataaacaagg tggaaacaga tgaaaaaaat cgtaatcaaa
     6121 ggaaaccgac ctttagctgg tgaagtaacg atcagcggtg caaaaaatag tgcagtcgca
     6181 ttgattccag ctgcaatctt agctgattcc ccagtgatct tggaaggtgt gccggacata
     6241 caagatgttc attcattaat tgaaatttta gagatcatgg gcgcaactgt ccatttttcc
     6301 aacaacatac tagaaatcga tcctcgagaa gtcgtatcga ttccgatgcc gcacggaaaa
     6361 atcaatagtt tgcgtgcttc ttattatttt atgggaacac tgcttggtaa atttggcgaa
     6421 gcggtagtag gcctgccagg tggctgttat ttaggcccta gaccgattga cctacacgtc
     6481 aaaggatttg aagctctagg tgctgaagtg acaaatgaac atggtgccat gtatttacgg
     6541 acagaaaata aaggattgcg tggcaatcgg attttcatgg acgtggtttc agtcggagca
     6601 acgatcaatg tgatgttagc tgctgtaaaa gcaaaaggac agacagtaat tgaaaacgct
     6661 gcacgagaac cggaaatcat agatgtggca actttactga ataatatggg ggcaaaagtt
     6721 cgaggagcgg gtacagatgt gatccgtatc gaaggagtcg aaacattaca tggctgtcgg
     6781 catttcatga ttcctgaccg catcgaagca ggaacctatc tagcattagc agctgcagta
     6841 ggaaatggta tcaaagtgaa aaatgtgatc tttgaacatt tggaaagctt tatagcaaaa
     6901 ttgcaggaaa ttggtgtcca tatgaaaatc agcgaagatg aaatcgaagt ttacccctcg
     6961 aaagaattaa aacccgcaaa catcatgacc tatccttatc caggatttgc cacagatctg
     7021 cagcagccac ttactgcgtt actattgatg actacaggca cttcagaaat catcgatacg
     7081 atctatgcga aacgtgtgaa ccatgtgccg gaactagcac gaatgggcgc tgatattacc
     7141 gtggaaggaa atatgatcat cgtgaatggt ccaaataaac ttcacggcac agaagtcgtg
     7201 gcttctgatc ttcgggcagg tgcttgttta gtgattgctg gactattggc agaagggaca
     7261 acgacgattt acaatgtaga ttatatctta cgcggttatg atcatattat cgagaaacta
     7321 acagccttag gggcatcaat tgaaatgata gaggaagtgg gagctgaatg agcgattatt
     7381 tgacaatggc agaactagag aacaaaacgt taaaagaagt ttatagttat gccaaagaat
     7441 ttaaaattcc ttattacagt aagatgaata aaaaggaatt atctctagct gtgattcgtg
     7501 cacaagcaga gaaacaagga ttttattata tggaaggaat attggatatc gttggtcaag
     7561 atggttacgg ttttttacgg ccaatcaatt atggaccaag tgcagaagat atctatattt
     7621 ctgcctcaca aatcagacga ttcagtttga gaaatgggga caaagtggca ggaaaagccc
     7681 gtccacctaa agaatcggaa cgttattatg gtttgatgca cgtggaaagc gttaatggaa
     7741 aagatccgga agaagcaaaa gaacgtccgc attttccagc cttaacacct ctttatccgc
     7801 aaagacagct tcaactggaa acaacaccgc aaagactatc tacccgaatg atcgatatct
     7861 tttctccaat cggttttggt cagcgtggat tgatcgtcgc accgccaaaa gcagggaaaa
     7921 cttctgtatt aaaagaaatc gcaaatggta tcaccgaaaa ctatccggaa gtagaattaa
     7981 tcgttctact gattgatgaa cgaccggaag aagtaacgga tctagaacga agcgtgaaag
     8041 gcgacgtggt atcttctaca tttgatctgc agccgcaaaa ccatactcga gtttctgaat
     8101 tagtattgga acgcgcaatg cgtttagtcg aagataaacg agatgtcgtt atcttgatgg
     8161 atagtattac tagacttgct cgggcctata acttagtcgt tccaccaagc ggacgtacat
     8221 tgagtggcgg gattgaccca gccgcattgt ataaacctaa gaaatttttc ggggcagcaa
     8281 gaaacatcga agaaggtggc agtttgacga ttttagcgac cgctttagtc gatacaggaa
     8341 gccgtatgga tgacgtgatc tatgaagaat tcaaaggaac tggaaatcta gaattacaac
     8401 tttcacgtga attatctgaa cgtcggatct tcccagccat cgatatcaaa aaatcaggta
     8461 cccgaaaaga agaattattg atgccaagtg aagaattaga agaaatttgg aaacttcgaa
     8521 aaaatatgac tggagattca ttagaatata cggaacaact gatccgcttt ttgcgtaaaa
     8581 caaaaaacaa tcagcaattt ttcaaagaat ttcaagatgt ctcctttggt aaaaaaaatc
     8641 cgaccagaaa tctgaaaaga taattgcaac cttgactgaa acatgttaag atgttaatgt
     8701 tgtaaatcga caaatcaact ctggttcagc aaatggagca gggcaaagga gcgaaaaaat
     8761 catgaaagaa aacatccatc cagattacca cccagtagta ttcatggatt caacaactgg
     8821 tttcaaattc ttgtcaggtt caacaaaaac ttcacaagaa acagttgaat gggaagacgg
     8881 taatacatac ccagtcatcc gtgtcgaagt aacttctgac tcacatccat tctacactgg
     8941 acgtcaaaaa ttcacacaag cagacggacg tgtggaccgt ttcaacaaaa aatacggtct
     9001 caaagacgca aacgctgcgg aataataaaa acgctgataa ctcaacgttt agcagactat
     9061 ctgataggga tagtctgttt ttttatgttt tttgccaatt taaaacttcg tttgatgctg
     9121 aaaaagtcgt aggatcataa tgcacaaaac aatgaaaaac tagtatatag ttttaattat
     9181 ccacttgata aaaacacctt atattaacga aatgaagagg ggttatttcg aagtaaaaaa
     9241 ccatttacct aaaattagaa gctaagcttt cctttcaagg aaggcttttt gttttgttat
     9301 agacttgcga ttgtttgtga gctagatcgt tcattttaat gacttataca cgcggatgat
     9361 gggtcttttt cctctgttca ttcataatat tgaacggtac agctagcgat gctatactga
     9421 cctcagttcc aagaaaacgt taacattttt tgtgggggag aggtttttta tgaggtttga
     9481 tcaattgatc gcagaagaac tgattgtttt cgatccatcg atagattcaa aaggatcatt
     9541 atttgagcga ataggggatg tgttgaaaaa aacaaataag gtaagaaatg ttcaaaaagt
     9601 cgttaaggca ctattcaaac gagaggagga gatctccaca ggaatagaag atggatttgg
     9661 gattccccat gcaaaaagca aatatgtgac aaaaccagca ctgtgttttt ttcatacagc
     9721 caaattaacg gactatagcg ggatagatga aatgccaatt gaatatattt ttgccatcgt
     9781 agttcctgaa aaatcagcag atatgcatct agagattctc agcacactct ccagacgatt
     9841 gatgaatgca gattttagaa agggaataaa agaagccact acgccagcag atatcatcag
     9901 acagattagt caagaatgaa aatagtagag tctttgtccg agtatcgata gtgaagaaaa
     9961 aggaggaaaa taatcatgaa aatcgttggt gtaactgctt gcccgactgg aattgctcat
    10021 acttatatgt cggcagaaaa attagaagta acagcaagaa atctaaacca tcaagcaaaa
    10081 ttcgaaacac aaggtgtaaa aacagaaaat cagttatctg aacaagaaat caaagaggca
    10141 gatgcaatca ttcttgcagt agataaagaa atcgaattgg accgatttgc cgggaaaaaa
    10201 gtaaagcgtg tctctacaag ccgagcaatc aaagaaccgc aagtagtaat agacgaagca
    10261 ttgagagata ttggcgtatt cgttgtgtct aatgaaaaag aaccagctgc aaatgagaaa
    10321 aagccgacaa tctataatca tttcatgaat ggtgtgaatt acatgcttcc atttgtgatc
    10381 gcaggcggaa tcatcatcgc actgagtttt gcctttggga ttacagcggc tgatcccaac
    10441 tcagcagact ataatgtgtt agcagctgca ttttcaagga tcggaggaga tacagcgttt
    10501 gccatgatgg ttcctgcgtt aggcgcgggg attgctacat cgattgctgg aaaagcaggt
    10561 tttgccccag gaattgttgc cggattactg gcctctacag gtggttcagg ttttcttggc
    10621 ggaatgattg gtggcgtgct agcagggtat atctgtgatt ttctggcaaa taaaattagc
    10681 gtaaaaaaag aattttctgc tatttatcag ctgattgtcg tccccttttt atcgatctta
    10741 attattgggc ttttgatggt atttgtgatt gaacaaccga tcacatgggt gttggatgct
    10801 ttgacaaatt ggctgaatag cttgggaaat acatccgggc tattgttcgg attacttatc
    10861 gggatcatga tggcagctga tatgggtggg ccgatcaata aatcgacttc gactttctcg
    10921 atcggtttga tgtcagctgg agtaaatgca ccgattgctg cttgtatggc tgcaggaatg
    10981 acacctcccc taggtattgc tttagccact ttattattca aacataaatt taccaaagaa
    11041 gaacggactt caggtcaatc atgctggatt ttaggcgctt cgtatattac agaaggtgct
    11101 ataccgtttg cggtatctga cccgatccgc gtgataccta gtttgatgac aggttcagca
    11161 gtcgctgcag ggatttccat ggcagtagga gttacttcaa tggcacctca tggagggatt
    11221 tgggtgatgc taattccaaa tgttatgaac aatttaccta tgtatgtctt ggcaattgca
    11281 tcaggtacag tcactacagc ctttatgctt ggactattga aaaaaacagt gaaagagaaa
    11341 aaacaagaca agtggacgaa aaatgaaaat ctaacaaatg aggtggaagc gtaatgttat
    11401 atacaatgaa agagttattg aaagtggcta aagagaatca atttgcagta ccagccttca
    11461 atatttgcag ctatgatatg atgaaagcaa tcatcgaaga agcagagaga ttagaagcac
    11521 cagtaatctt agaaattcat cctgatgaaa tcgcctattt aagtgatgag tttgttgcgg
    11581 cagtaagagc ctatgcttat acaagtaaac tgccgatcgt tatccacttg gatcatggcg
    11641 gtacgataga agatgtctta cgggcgattc gaaacggcta ttcttccgtg atgatcgatg
    11701 cgtctttgaa atcattcgac gacaatgtac agattacgaa aaaagtggtt gcgttagctc
    11761 atgcggtaaa tgtctcagtg gaggctgagc ttggaacgat tgggaataat ggttctgctg
    11821 aaggaggtgc tgaaacaatc atttatacag atcctgatca agcgaagatt tttgtgaaag
    11881 aaacaggaat cgatacactt gctgtagcaa ttggaacagc gcatggcttg tatccgaaaa
    11941 acagaactcc aaaattaaat atcgacttac tgaaagaatt gaaccaacaa atcgatcttc
    12001 catttgtttt acatggcggt tcaggtaatc cggacgcaga agtcagcgaa gcagtaaaat
    12061 acggagttgc caaagtgaat ttgagctcag atcttaaaag cgtctttttc aatgctttga
    12121 gagaaatatt gagtgaaaat ccatcgatgt acgagccgaa tgcaatttat ccagcagcta
    12181 ataaaaaggt acaagaagtg gtcaaacata aaatggaaat tttgaacaca gtaggcaagg
    12241 ctagtttata ctaaaaataa aaatgtgtgg tcaagagggt atgacaatag tcttgtcata
    12301 ccctcttaac cacacacgat gttcagaagt agttttttcg aaaaatttct tcttatttct
    12361 cgaagccaat cacttctgtc acaacctctt ttttcctgtt actatataaa gtaaatgggg
    12421 tgattttatg ttgaatgaag tggagaatag acagcaggag attgtgaatt atctaaaaaa
    12481 caaccaattt gcaagagtca ccgatttgat cgagttagta aattatagcg aagccacgat
    12541 taaacgtgac cttgtttttc tggaaaaaag tggattgatc cgtagagttc gcggtggcgc
    12601 catgcttatt gacaatcgaa aaattgacgt gccttacctg atgaagatca ccaaattaga
    12661 agaagaaaca gaaaaacgta agattgcaga tacagcttct tctttgattc gagatgatat
    12721 ggtcttattc ttggattcca gtacaacctc tttacactta gtcagaaacc tagcaagatt
    12781 tgatggtttg cagatcatta cgaatggcat cgtcacagct gctatgttga gtgaatatac
    12841 aacggcaaaa gtatctgtat tgggaggggc aattgttccg aaacgagcaa caatcaacgg
    12901 ttcaaaagcc tataacgatg cattgtcata ttatgcggat ttagcgataa tgagttgtcg
    12961 cgggctagat ttcagccaag gagtaacaga aactcatgaa ggagaggcaa tggtcaaaaa
    13021 agcttttaga aaacaatcag accggcttgc cgttttagtt acacagcaaa aaattgcaca
    13081 taaattcatg catcaagcca ttgcttttca tgaaatcgat tatctggtga caagtaaaga
    13141 actgacaaaa gaacagtatg cgaaagtaaa ggaacaccat atcgaatgtc tgtacggata
    13201 aattattacg tgaatgttct aagtggcttc ctcattttta gataggacct tatctttgct
    13261 cgcttctctc agccgatggt ataataatga aaaggttatc ttgttagata atcttacttt
    13321 tttatccagg agaaaggagt gcagaaaatg aaacatgtga aggcgttggt agtgaaggca
    13381 atcatgatct gggcgattct gtgggtcgtg ctgacaggtt tatacggtgt tagctttatg
    13441 gattcaacga ttgttggggt aattattgta gtcatgatct atgtgctagg ggatctgttg
    13501 atcttacgta aagtagggaa catcgcagcg acgatcgcag atgctggttc agctgcagtt
    13561 atcttgtggc tctatctata ttttatgaat gatacagtcg atatttggat gaaagtattg
    13621 attcctgctc tattgatcgg tgtggcagaa tggttcttcc acaagtggtt gctaagtcaa
    13681 ggcatcgtac cagatgaacg gaaaatggaa taaatcaaca gttaaactat tcttatcgat
    13741 aaatgaaagc tttaaaaata aatagatgtt taattaagaa taatcataaa ctatttttac
    13801 tcaaaaaacc gctcagatta ttttcagatc tgaacggttt tttttatcta ttcatcaaga
    13861 tcaaatttca tttttcaatg attcaatcaa ttcgccctct gcttcatgac tatgttttcg
    13921 gccattaaca agatagagtg ctgtacatat caatggaatg actccggcaa tcacatacaa
    13981 tcctctaaat gaagtaagag attttagcat ccccatcaaa taaggaccaa cgcctaagcc
    14041 taggtctaaa ccaataaagt aggtagataa agcaatactg attcgatgtt cgcggacgat
    14101 ttttaaacaa acagcctgtc catttgacat aaacgtacca taacctaggc cgactaatgc
    14161 tccagagata agcaatgtca cactattcgt cgttacactc agtaaaaata atcctgctgt
    14221 taagaataaa tagctaggat acatcacgta ttgttctcct ttgacatcga aaatccttcc
    14281 agtcaatgga cgtgtaaatg tgatgaccaa agcatatacg acgaagaaaa atgacccagc
    14341 agcaactaag tggattactt ttgtataaga agataaaaat gataaaacac ttgaataaga
    14401 aagacccatc aaaaaggcaa ttcctgtaat gaaaagtgct ttcttttcaa taaaactatt
    14461 gatattccat gagcgaagag atattttttg ttcttcagat aatggaatat tttttacagg
    14521 aaagacaagg caggcaatcg taacaaagaa gaccagtaca attgaaaacc agataatgaa
    14581 acgaaaatca gtcatattca gcaaaatcat tcctaaaaat ggcccaacag cggcagctaa
    14641 actagtactt aatccataat aattgatgcc ttcccctttt ttagatgcag ggatatatgc
    14701 agtaacgata gcatttgtag ctgtagaaac tgtaccgtag ccgaatccat tcaagaatcg
    14761 aactaaatac aatgccccga tagttggcgt ataaagatat gctaatgttg aaaggagata
    14821 gaagatcgcg ccgcctctta aggtaaactt ccgaccaaat agttctaact gtttccccat
    14881 gaataagcgt gccagcaatg ttccgatgat atagatccca gaagcaaaac ctgcttggct
    14941 caagctggcg tgtaaggtgt cttgcgcaat gactgccatg atgaccatca acagataata
    15001 aatcaggtag acaataaaat tgatcaatgt gattccaata aaatatttat taaataattt
    15061 cttttccatg tatgttatac attcccctta agctgaatat gcttttaatt tttgtatttg
    15121 aacttctaca tcgtgataat tggatggata gcgatcaaca agttggcgaa taaataaatc
    15181 tcttgtagac ataaacagat ttaaaaattc ttcgcattcg tttgttggat tctcatccac
    15241 tgctttgatc aattcgatcg agttttgata cgcatcattt aaaaagacta aagaagcttt
    15301 catatcaaat cttgtttgat atgctgtttc taatcgatta atgattgtgt tcatccagta
    15361 attcatgttt tttccctcta ttcttaaaaa attttacttc tgcagtataa cgaataaaaa
    15421 aaaggaaaaa aatcgcataa gtgatggaaa aaggtactta acaaatgtta atatcattag
    15481 taaaatacct ttggacttta aaaagtttta aagtactgta tgatcttttt agatgaaagt
    15541 ttggatgggg gagcatcatt ggatagtaga gtcttaaagg aatatttgga taaaaatgat
    15601 tttccgatta tacgcaaagg atatcataaa tatttgacat tcgaagggat ggaagattcc
    15661 catacgtata tcttgaaaga gggaacggta aaagcaagtg ttatttcaaa tgatgggaga
    15721 gaatttaacc ttcgctatat caaagatctt gaaatcgtct ctcttttaaa agacgaatat
    15781 tcacaattca tcgattcgcc gtataatatt cgagttgaat cagaatatgc agagttttat
    15841 cgaatcaatc gtgtgcgctt ttggcaaaat atcaaccaag acccaaagct acaacactat
    15901 gtaaaagagt attaccgcta tcgtctgctt tattcgatga aaaaaatgca gcaaatgcta
    15961 ttaaacggaa agctgggagc tgtttgtacg caactttacg aattatatga tttattcggt
    16021 gtgcggacag aagaagggat gctgatcgat ttttcgatca cgaatgaaga aatcgccaaa
    16081 ttttgtggca tctcaactgc tagcagtgta aacagaattt taaaacaact gaaaaaagat
    16141 ggtattatca ccgttaaaga ccatcatatc gttattttga atatggactt gttagaagac
    16201 aatattatct tttgatgaac aaagaggttg tgaaatcagc gttttgacct gagtattaga
    16261 gaaacaaacg gaaaaatagt tcctcatatt ttgccgtttt ttgagctaat accgagggtc
    16321 agctgattga acaccgttta ccaagaggtt gtgagcctgc cgtttaactc tgagtcacaa
    16381 ggaacaattt caaaaaacag cttttcatgt ttgatgaata ttgtgacttg tgaccgagga
    16441 gttggtagga gaacaccgtt taccaagagg ttgtgaaaaa gctgttctgc atcaagtaat
    16501 aagaagagcc aagtaaaaac agtctcttat gtttttaact tggctggact tatcaccgta
    16561 gatgtaagct tttgaacgcc gtttatttgc aacaaaaggg gctgtgacac gatttttgtc
    16621 acagcccttt ttttgcgaac attcatttag caatttttag aaacttctgc cgccaccgcc
    16681 aaaagtcccg ccaccgctag aatgggtagt acttccgcca ccgccgcttc caggaggtgg
    16741 gcttttgggg attcttcttg tcgtgacaaa agaatttgtc agacgatcgg ttttttcagt
    16801 taatttgata ctggattttt ctcggaatgg atatttgtag atgcctgatt tcaattgata
    16861 acgcgaaaca gtcacaaaat aaaagacaag actaaggata agagccaaaa caacagcaat
    16921 gatgatttct actgtcgtca aaaccttata gcgtgtaatt ttgccagttt cttcgtctac
    16981 acgataatgt cctcctggaa ctccgtcttc cacatatgtt gctgatttag acaggtaagc
    17041 ttttgcagca ttgtaatagg cagcatttgt catctgatca taaacatcat ctaagatcga
    17101 atcgattctg ctatcagtca aataatcgat catattacca gaagtcgaaa tgtagatttc
    17161 acgctgtccc atatctaata gcaatactga gccattttga tcatttccga ctgcgtctct
    17221 taagtaatta tcagcaaagt ctcttggttc ttctgaatta gatgtagtcg ttacgatcaa
    17281 gactcgtcct ttgatttttt cattcagctt ttctgcctgt tgttccaaag attggatttg
    17341 atctgttgtg aataacccag cctcatcatt taccgtagct gtatctgcat gagtagccac
    17401 cggccagcca acacttacta gcaaaagaaa gaataaaaaa aactttttca tcatatcaag
    17461 taacctccga tcagaaagag gatcgctagc ccagcgaaaa tcccaagagc ggtcagtccc
    17521 aattttttct ggctgatcgg caggacaccg ctgacctttc ctgtttgtcc attcattgcg
    17581 tagtagtaga cttttttact tgagtcattg cttcgataag taacaagcca taccggcagt
    17641 aatacgtaat tatttacttc gtttgtgatt gaagcagaag ttcgcacacc ggtcaatgtt
    17701 gtatatccat ttgcggtatc ttttaacagc ttctctgaat agtcttttaa ttcagattga
    17761 atctgtgatt tgattgcctc atattcgata tctctttttt ctgcttggaa cccggctaaa
    17821 tactggcttt taaaatcaat tgctttatct aacgggaaag gttgcacgcc ttcgaccatc
    17881 ttttgttgcg tattttttga taaggcattt tttactagtt ctttaaaaga aagcttgcct
    17941 gctcttgaaa cagcaaattg cttcgtttct gtgtattcga tgtcacctat ccgccatatg
    18001 cggataactg taccattcgc ctgcatggca ccatctactt cagcatctgt agcccaataa
    18061 gggaaataca cacctgtcaa cttatcgatt tgatccttat tgaaaaaagc tttgggaaca
    18121 aaccattttt tcttggtcca agcgagaaat ctgttgattg cttcttcttt ctcgacagca
    18181 aaaggaagta ctttatttgg caagaattct ccgcttaaac gtcccgatag tacgacggga
    18241 ttatggcagt agtaacaata agtcgcagca gttgtcgcat cagtcacgat ctctgcacca
    18301 cagttgggac aaagaaaaag atctatcgct gcggtattat ctgcagattc gtccgctgta
    18361 tctgaaaaac cgcctgcctc gtcaaccgct ttgcgctcgg cttcattcat atcggcaagc
    18421 tgttcttggg ctgtaaaagt aaaatcttcg gtaggtgaag gttcttcttt gctttcttcg
    18481 ccagccatac gcgcttcttt ttgcttttgc tcatattggc tgacttcttc ttctgtatag
    18541 atattcaggc agtattcgca atgaaatttt tggtctttgg gatcaaaagt caacggaccg
    18601 ccgcaattag gacatttatg tgtaagaaca tccatcagct gcacaccttt ctaaataaat
    18661 taattaacgg ggattccttt aaacggttta ccacagtttg gacagaattt agggatgccg
    18721 ttggaaagat ccacgatctc attgcattca ctgcatcgca tttgtagctt aggctgaacg
    18781 ttttctacag gtttggcagc tccgcagttg gaacagaatt ttccggaatt ttctgtcccg
    18841 caaactggac atgtccaagt atccgcattt ccttgtgcag tttgttgatt ttgtttttct
    18901 gcttgttgtt tgatttgttc ttggttattt tgagctgctt gagaaagata agaaccattt
    18961 gcattcattc ccatacctac acccataaag ccggtcattg ctcctgcttc attttttccg
    19021 gctgcttcca taccacgtgc aatggaacct tgcacgtatc cttcacgtac gctaggatca
    19081 cctagcatcg ccccttcgtt acgcatattg atcagtttca cagaatcatc ggtgtaagaa
    19141 atgctggcta cagccacaga aacaatctcc atcccacgta attcacgcca agaatcatcc
    19201 aatgcagtat ccatgtattt gcttaattcc aaactcttag atggtacata agaaatccgt
    19261 tgaccatctg cagacatctg attgattgct gattgtagcg ctgttaggaa ttcagctaaa
    19321 tactgctcat tgatatcatt gatttccact tgtgttttgt ttttagggat tgcgtttgta
    19381 taaaataaaa tcgggtcaac gacatgaata gaataagtgc catgtgcccg taagaataat
    19441 tctgcgttat agaaattgtc aaagtagttc aacggcgcgc ttgtaccgaa tttgattccc
    19501 tttatttctt ggagattgat ataaaaaact tgttgtttct gcggtgtgac gccgccaaat
    19561 ttgaatcggt caaaagtttc agcaattgct tctttcagtg ttccattgaa catggaagga
    19621 gctgcgtcat ttttaatggt gtaataacct tcctcagcgg tataatcgat gatttttcca
    19681 ccatcgacca aaagcatcat catgtttgga tagacatgga cgactgttcc gtctgttaaa
    19741 acatcttctg ttcctttacg attggagcca cgtttgtcat ctttgcggac tttgacacct
    19801 tttgtcatga ccgttgtatc actcatattg tctggttcga tgacttctag ccattgatct
    19861 gccagaccgc cgccaaccat acttgttgct gctttgataa gacccataaa aaattcctcc
    19921 tctgattcag ctagtgccaa acaattattg tttatttatt ataccatagg aagatgtgaa
    19981 acttctcgga ctagagacgg aattgaaatg gaaaattgcg tattgtttaa atacctgaaa
    20041 aagccgagaa ggatagtagt ttttttacct aatatcacgt ataataagag atagtgaatt
    20101 ttttaaaaaa gggggatcct tatggcatat caagcacttt atcgagtctg gcgttcgcag
    20161 cgtttcgaag acatcgttgg acaaaaagct gtgacacaga cactaaaaaa tgctattgtt
    20221 tcccacaaaa cctcacatgc ctacttgttt acaggaccca gaggaaccgg aaagacgagt
    20281 gcagcaaaga ttttcgcaaa agcaatcaac tgtccgaaca gtaaagacgg tgagccatgc
    20341 aacgaatgcg aaatgtgtcg atcaattact gctggtacgc aagaagatgt gatcgaaatc
    20401 gatgctgcca gcaataacgg agtcgaagaa atccgattta tccgtgatcg ggcaaattat
    20461 gcgcctacaa aggcagcgta caaagtgtac attattgatg aggtacatat gctttcaaca
    20521 ggagctttca atgccttgtt gaaaacattg gaagagccaa aagaaagtgt catattcatc
    20581 ttagcaacga ccgaacctca caaaatacca gctacgatca tttcacggac tcagcgattt
    20641 gattttaaac ggatcaatac ggcagatatt gtcgaacatc ttgaatttat cttgaaagaa
    20701 tcgactatca gctatgaacc agctgcattg aatgtgattg cccgttctgc agaaggcggg
    20761 atgcgggatg cattgagtat tcttgatcag gcaatctcgt ttagtgaagg aacaattact
    20821 ttagatgatg ccatgcaagt gacaggtagc ttaactgacg aaatgatgga cagttacttg
    20881 agtgcttgcg tgaaaaaaga agtaccagaa gcattggaaa cattgaacca aattctcgca
    20941 gccggcaaag aatcacgtcg ctttctagag gatatgttac aatactgccg tgacttgctg
    21001 atgtatcagc aagcacctaa cctagttgag gaacaatcga gtacgatcag aagtacattt
    21061 acagaattgg cacaaactgt ttcagcggat cagctgtttt caatgataaa gattttgagc
    21121 gatactcaaa atgaaattcg ttttaccaat agtgccgcta tttatttgga agtcgctaca
    21181 gtcaaacttg cacagctacc gtctgcttct gttccttcag cagatgtagc atttgcagat
    21241 gatcagatag taaaacagct acaacaagag attaaggaac taagaaacga aatcaaggaa
    21301 ctacgcagta atacaggaat tgagaataca aaagtacagc cggtacagca aacaaatcat
    21361 aaaaaacaga caagtaccta tcgtgtaccg acggaacgag tatataaagt tctgcgggaa
    21421 gcgacaagac agcatctgaa ccaagtaaaa gaagtatggg aagacttgct gatgagtcta
    21481 tctgtcactc agcgagcaat gcttaaagca agcgaaccag ttgcagctgg acctacagga
    21541 ctagtgattg cgtttgatta tgagattgtt tgtcaaagag cagcaaatga cgaagaattg
    21601 cagctttcgg tacacaacaa cttaagccga atgatcaaag attatgcgcc tgatcctgtt
    21661 tatattacaa gagaaagctg gccagaactt cgaagaaatt atttgacgca ggcacaagaa
    21721 gagggaacag aaaactcttt tgcttctgaa gactcagaag gagaaatcga gttgcttcct
    21781 caagaacaag aagtaccaga agaaacactt gtagtaaaag cagaggaact gtttggctcc
    21841 gaagctgtta cagtggtaga tgattaataa aaaggagtag ataattatga tgcgtggaat
    21901 gggcaatatg caaggcatga tgaaacaagt acaaaaaatg caaaaagaaa tggcagaagc
    21961 acaagagctg ctaaataaaa cagagtttac aggtaaagcc accaatgaat tggtcaaagc
    22021 ggtttttaca ggcgatcgtc gcatgaaaga tatccaaatc aatgaagaag tcgttgatcc
    22081 tgatgatatc gagatgcttc aggatcttgt cgtaatggca gtcaatgatg cgatcgcaca
    22141 aattgatgtg gaaactgaga aaaaattagg gaaatacgca aaaggtttgc caggatttta
    22201 gttttaaact gaaggagtaa tgaatatgca atatccagaa ccaattgcga aactgatcga
    22261 cagctacatg aaacttccag gaatcggtca gaaaacagcg acaagactcg ccttttatac
    22321 gatcgatatg aaagatgaag tagtaaatga atttgctaaa tctttgttaa gtgtaaaacg
    22381 cgatttgcat ttttgtagta tctgcggaaa catcacagaa gaagatccct gcgaaatatg
    22441 tagaaatcct tcacgtgatc gaagcgttat cctagtggtc gaagagccga aagatgtaat
    22501 ggcaatggaa aaaatgcgag aatatcacgg actttatcat gttctccatg gtgtgttatc
    22561 accgatggaa gggacaggtc cagaagacat caatatttct tctctgttgc agcggctgca
    22621 tgatgcagat gtaaaagaag tgattatcgc aacaaatgca acaacagagg gagaagcaac
    22681 agctatgtat ctctcacgtc tgatcaaacc cgcaggtatc aaagtgactc gtctagcaca
    22741 tggtctgtct gtaggctctg atatcgagta tgcagatgaa gttactttat tgaaagctgt
    22801 cgaaggaaga cgtgagttat aaatttctaa ttaagtgttt tttcagatac aatataaaag
    22861 cagcacaaga gatgggggca acaatgtgaa cggactattt attacaatcg aggggcctga
    22921 tggtgcagga aaaaccagca tcttgaatga actttaccct cttttgaaag aagtagcaaa
    22981 aacagaaatc attcaaacaa gagaaccagg aggcattccg attgctgaag agatccgagc
    23041 agtgatcctt gatccaaaaa atgatcggat ggatgaacgt acggaagcac tgctttatgc
    23101 agcagcaaga agacaacatc tagtcgaaaa ggttctgcct gctctggcaa aagggaaaat
    23161 cgttttatgc gatagatttg tcgacagttc tcttgcttat caaggtgcag gccgacgcat
    23221 cggcgttacc gagattgctc ggttgaatga gtttgctaca gaaggtacta cgccggattt
    23281 taccctttat cttgacgtag attcagacac cggattaaga agaatcaaga aaaacagaca
    23341 aaatcaaatc gaccgtttag attcggaagg tttggaattc caccagcgtg tacgtcatgc
    23401 ttacttaaag ctggcagaag agaatccgga aagaatccac aaagttgatg caagaaaaag
    23461 ctttgaagaa gtgctgcaaa caagttatca tacaattatt gaacagtatc cgcaattttt
    23521 tgaaaactag gaaggtgaac gtttatgaaa attattttag cgattatcca agacaaagac
    23581 agcaaccgtt tatcaaatga attgattgat gcaaatatcc gtgccacaaa actatcatcg
    23641 acaggcggct tccttaaggc tggaaacagt acgtttatcg tgggtattga agatgaccgt
    23701 gtagaagaag cattggaaat catcaaaaaa acgtgtgaat caagaaagca atttgtatct
    23761 acacctgtta cactggatat ttcgatggat gggggcgtcc cttatccagt agaagtagaa
    23821 gtaggcggtg ccacagtctt cgtcttacct gttgaaggat tccatcaatt ttaggaggag
    23881 ctgagctatg aatcaagaaa taaacttggc tcaaatgcaa ccgatcgtgt atcagcagct
    23941 tcaacggagt tttgagcatg ggcgtatcgc tcatgcttat ctttttgaag gggagaaagg
    24001 aacaggaaaa catgaagtag gtatatggct tgcacagcac ctgttctgta cccaaatgaa
    24061 agacaatctg ccttgtggaa aatgcaacaa ttgtcagcgg atcgaaaaga aagaacatcc
    24121 agatgttttg gttattgaac cggaaggaca aaccatcaaa gtcgatcaga tcagacgttt
    24181 gcagacggaa ttcagtcgca gcggttacga atcaagaaaa aaagtttttt tgatcaaaga
    24241 agctgaaaaa atgaatagca gtgcggcaaa cagtttgttg aaattcttgg aagagccccc
    24301 tggagatttt ttagcggtgt tagaaacaga tgcagtagga agaattctcc ctactattca
    24361 atctcgttgt caagttctgc attttcaaga actttcgaaa gaagcactga tccatcgatt
    24421 gcaagaagag cagattccat tggaaaaagc aaaacttttg gctttcttaa caaacagttt
    24481 aggaaaagca gttgaaatct ctcaagatga atggtttaat gatgctaagg acttgattta
    24541 tcaatggttc gtttacttgc aaaagcaaga tactcaagcc tttatctatg tccaaaaaaa
    24601 attggtaaaa tcatttaaag aaaaaacaca acagtttaca ggattatctg tcttgatgtt
    24661 ttattatcag gaagcacagc gtcaagcagt agcaaacggc gaatatggca aagtgaaaca
    24721 gataaaccag ataattgaac ggatattgat cgcagagcag aaactacgca gcaatgtcag
    24781 ttttcaggct gttgcggaac agttcgtttt gcatacgatt ttttagcttg ccgcaaagga
    24841 tgatgatcaa ggaggatgga aatggtagaa gtagtaggtg tccgatatag agatgcaggt
    24901 catatctatt attttgcacc aggagactct gaatatgttt atagtgacaa ggtattagtc
    24961 gaatctcagc aatccttaca tattgctact gttgtccttc ctaaaaaaga gattgatccc
    25021 aatgaccttc ctgaagctat ccgtccgatc ttgcataaag cgtcacaagc tgaattagaa
    25081 aaagacaaaa aaaacaaaaa agatgcttat gatgcattga aaatcgcaaa agaaaaagtg
    25141 cagttgcgtg agttggaaat gaaaatggtc aaagccgaat acacatttga tcgaaataaa
    25201 ttgatttttt ctttcacagc ggacgggcga attgacttta gagaattggt aaaagatctg
    25261 gccgctgtat ttcgtactcg tattgagctt cgacaaattg gtgtccgcga tgaagcaaag
    25321 ataataggcg gaattggtcc atgtgggcgt ccattatgct gttcttcttt tttaggcgat
    25381 ttcgtgcctg tatcaatcaa aatggcaaaa gatcaaagtt tatctttgaa tccaacaaaa
    25441 atttccggtt tatgcggacg cttgatgtgc tgtctgcaat atgagagcga agaatacgag
    25501 acagcgaaaa aagaactccc tgactttgga aaagtaattg ctacacctga tggtaaaggg
    25561 aaagtcgtag ggttgaatct gcttagccgt atcgttagta ttcggctgac tggacgggaa
    25621 aatcctgtcg aatatgaatg ggatgaattg aaagaattta tccaagtagg tgagatgaat
    25681 ggataagaga tcattatacg acggattgaa tcaattagaa tcagagttga gaaatacatt
    25741 aacagaactt gttgaaatga agcatgcttt gcatgagatc gtagaaaaaa atacaacttt
    25801 ggagatggaa aatcaacgtc tgcgggaaca cctgcgggaa ttgaaccaag ttgcacaagc
    25861 tccagctgaa aatgtgaagc aggaactttc taagtctcgt atgaatcttg aaaaaattta
    25921 tgaagaagga ttccatgtct gctacgattt atatggatca agaagggaca atgacgagcc
    25981 ttgtgcgttt tgtctagaag tgatctatcg cgaaagcggt cgatgaccaa taacaagagg
    26041 agactggagc ggagaaatac tgctccagtc tttttattaa ggagattgtg acagatgtat
    26101 aatcaaaaaa gtttcaaggg aaatccggta tatggggatt tatatcttgt accgacgcca
    26161 attggaaatt tagaagatat gagctacaga agtgttcgta tgctgcaaga atccgatctt
    26221 atcgcaagtg aggatacgcg taatacacaa aagttattga atcattttga aatccataca
    26281 ccccaaaaaa gtttacatga acataattat aaagaacgtg tccctcaatt gattgctttg
    26341 ttaaaagaag gaaaaacgat tgctcaagtc agtgacgcag gaatgccttc catcagcgat
    26401 cctggacatg agctagtttt agcctgcata aaagaaggga tttctgtgat ctctatccca
    26461 ggacctacag ctgggatgac ggctttgatt gcttcagggc tagtgcctca accattttta
    26521 ttttatggct tccttccacg taagaaaaaa gaacagaaag aaacattgaa aaagctaaaa
    26581 gaacagacag ttacgctgat tttttatgag tcaccttatc ggatcactgc tacattgacg
    26641 aattttttag aaatttttgg aaatcgccaa gtcgttttgt gtcgggaatt gacaaaggtc
    26701 catgaagaat accttagagg aagtacggaa gagctactcg cttatatcga agaacatcca
    26761 gtaaagggtg aatgttgtct gcttgtagaa gggaatttgt tcgaaatgcc agtgacggaa
    26821 gttgatgaag agttgggaac gttaaaggaa caagtagaag caaagatcgc ttcaggcgaa
    26881 aaaccaaatg ctgctatcaa agcagtagcg ctgaaaaatg ggctgaaaaa acaagaagtc
    26941 taccgtcagt accatgaatt agactgagat agtataagtt gtctataatg aaagactaat
    27001 gaggaaatca gctactaact tttcaaatct cttgaacgcg aacgaatgtt cttgtataat
    27061 gaataagagg tgaggatgat ggagctgcaa tttcctttac ataatgatac atcaagaaaa
    27121 atcattcata ttgatatgga tgcttttttc gcttcggttg aagaacgaga tcatcctgag
    27181 tttcgcggac atccattagt aattgccaga caccccagtg acactggtgg gcgtggagta
    27241 gtgactacag caaattatga agcaagaaaa ttcggaattc attcagcaat gagtgcacaa
    27301 aaagcgtatg agctttgtcc gcaagccatc tttaagcctg gcgaccatca taaatacagc
    27361 gatatttcca aacaagtacg ggaagtattc tatcgttata cagatattgt ggaaccagtt
    27421 agtatcgatg aagcgtatct tgatgtgacc gaaaataaaa tcaattgcaa gtcagccatc
    27481 aaaatcgccc gaatgatcca agcagatatt tgggagagtg tccagctaac atgttctgcg
    27541 ggagtaagtt acaataagtt tttggccaaa ttagcatcag actatcaaaa acctagagga
    27601 atcacggtag ttgcccctca agacgctgtg ccatttttaa aagctttgcc aattgaaaaa
    27661 tttcatgggg tagggaagaa aactgtacct agaatgcatg agctagggat ttatacagga
    27721 aaagacctat atgaatgcac agaaatgatg ttgattcgaa attttggaaa aatgggctat
    27781 tctctttatc gtaaagtcag aggaatccat gattctccag taaacgttac aagagagcga
    27841 aaatctgtgg gaaaagagca tacttatggg cagccgttgc aaacggaaga agcagtgctg
    27901 actcagttgc gccaactagc tgaaaaagta gaagaagcac tgagaagagt ccaaaaacat
    27961 gggaaaacag tagttttgaa ggtcaggtac acggactatt ctactgtgac caaaagagtg
    28021 acattgccag aatatatcta taaaaaagaa gcactctttt atcaagcaag ccttatatgg
    28081 gaggaaatct tgggagtaga aaaaggaatc cgattgttag ggattacttt gacgaatctg
    28141 gacccgatga cttatgaaaa catcgtcctt ccattatggg aaaatcaaga aatataaaaa
    28201 cagctttgag agttgaaacc ggaaatccgt tttctctccc aaagctgttt ttatatcaag
    28261 tcttgctttt tgatttgttc ataggtttct gttgcatccg tacatttttc ccaaatcaca
    28321 acttgattgg attctaaaga tttttggacg tcaatgatcc gctggttgct acttccgcga
    28381 aattgtagtt ttaggtcacg tttgcttaat tcaaagcgtc cgtcaacaag gatatcgatt
    28441 tgcgaaagga gttctaattt atcttctgaa tcttgtagca attcatcaaa ggcatagcct
    28501 gtccatgacc agatatcttt tgtcgtaccg aactcttttc ggatacgtcg tacgacttgc
    28561 agacaaacat ctgtattcaa aaaaggttcg ccaccaagta aagttaatcc ttgcacgtaa
    28621 tcgttgcgca agtcagcgat gatttgttct tctaattcag gtgtgaatgg tttgccatat
    28681 cggaaatttt gtgcagcttt gttgaagcag ccttcgcagg caaataagca gccactaacg
    28741 taaagactgt tgcggacacc ttcaccatcg acaaaattaa acggtttgta gtcagcaata
    28801 tagttttggc tgtagtcttt tgctaaccat tcttttggtt ttggatttct cataaaaaaa
    28861 cttctttcat ttaaggttca aagatatagc tattgaagat aaaatctact cgaccacata
    28921 aataagaaca aactgaccta aatccattgt tcaagaaagc ttgttcttat tttgatagag
    28981 ggcgttcaaa gatacaaagc catcgaaaga gggtggtgaa aaaagacttg tcatagcctc
    29041 ttattctgaa taaacggtgt tcaaaagctt gcatctgcag taataagccc aactaaacga
    29101 aaaatatgag gaactatttt tgtttagcta ttcttattac ttgatgcagg acagcttttt
    29161 cacaacctca tttcatgtgt ttcacacgtg aggaaatttc tttatggcgg ccatgtacca
    29221 tcggacgagc ttgcggattt cctaaatagc cacaagtacg tttgactaca tcacatgttt
    29281 ttggatcatg atttccacat tctggacatt cgaaccctcg ttctgtcggt ttgaaatcac
    29341 cttcgaaacc acattgatag caatgatcga ttggcgtatt cgtaccaaga tacccgattt
    29401 tgtcatatgc aaaatcccat acagcttcta aagctttcgg attttgttgg agcatagggt
    29461 attcacagta gtggatgaat ccacctgaac aatattttgg ataatccttc tcaaattcca
    29521 atttttcaaa tggcgttggg tttttacgca catcatagtg gaaactgttt gtataatatt
    29581 ctttatctgt gatattttcg atgatgccga atttttctgt atccaaacgg cagaaacggt
    29641 cagttagact ttcacttgga gttgaataga cactgaaatg ataaccatat tcatcccccc
    29701 atgcatctgc attagctttt aattctttga cgatatcaag tgtgaaagct tttgcttctg
    29761 ggtttgtttc ccattcgcca ccgaagaaag ctgaagcaac ttcatacaat ccaatgtagc
    29821 ctaacgatac agtagctcga cggtttttga ataattcatt gacagaatcg gtacgtgaaa
    29881 gacgtttccc gaaagaacca tacatatata aaattggcgc atttgctgga atggcttctt
    29941 tacaacgttc tacacgatag agaagcgcat ctttcatgat ttctaagcgg tcagacagta
    30001 actgccagaa tttattctga tctccttttg attcgatcgc gattcttggc aaattcaatg
    30061 taacaacacc taaattcatt cgtccgacat ttacttcttg accattttcg tctttccacc
    30121 cttgcaagaa tgaacgacag cccataggta ctttgaaact tccagttaat tcaacgattt
    30181 tatcgtaatt cagaatatca ggatacattc ttttcgttgc acattccaat gctagttgtt
    30241 tgatgtcgta attaggatct tcagggttta agttcacacc ttttttcaaa gtgaagatca
    30301 gtttagggaa gatagctgtc cgcttttcac tccctaatcc attgattcga atctgtaaaa
    30361 ttgctttttg aatttctctt tcaaaccagt tttctcctag tccaaatcct aatgaagtga
    30421 aaggagtttg tccatttgaa gtaaataagg tattgatttc atattccaaa ctttgcatag
    30481 catcgaagat atcttttttg gtttttgctt ttgcatattc tttttggcgt tctggcgaat
    30541 cgatccattc ttccgcgtct tttagatgtt ttttatagtt caattctgca aaaggagcaa
    30601 gcaattcatc tgttcggtcg gcagaacagc caccatattg acttgacgct acattggcaa
    30661 taatctgtga gatttgtgct gtggcggtct ggatagattt tggcgattcg acttcggcat
    30721 tgccgatttt gaacccattg ttcagcatgc ctttgaaatc aatcaagcag caattcgtca
    30781 tcggtgtata aggatgataa tccaaatcat ggtaatggat atcgcctttt tgatgcgcat
    30841 ttgctatatg tggtggcaac attttcagtc cgattgattt tccaacgatc ccggctgtca
    30901 gatctcgctg tgtattgaaa acattactgt ctttatttgc gttttcgttc acgacagttt
    30961 ggtccttgtt gattagcttt tcaatcgaaa agttgatatc tgttgcttta ctgcgagcaa
    31021 aatctcgttt ggtacgataa ttgatgtact cttctgctaa ttcatattcg tttttttcta
    31081 gtaatatatg ttccacgatg ttttgaatct catagatttt gacattttca gcaaaccggc
    31141 ttttgatttc ttggacaacc aagtctgcaa tcatttgatt tttttcataa gtaagtggtg
    31201 ataatgagcc gtgaattttc ttttctgctt tgatcagtgc ttcattgatc ttttgttcat
    31261 cgaaatcaac atggcggcca tcgcgtttga tgactttcat ctgggatgtc tctggctgct
    31321 gagtaacgac agttttttct tcataattta ctttcattat cttcaactcc tgctaaattg
    31381 ttcttgtcta tcataaaaca aaatcgggga aggagcaact aaatatcggc tatatatagt
    31441 attcaatgtg ctgtataatt ggatttctat actatatgta gtgtatattt tattgtgaaa
    31501 aaaggtgaaa acttgtagct ggtggatttg ttttttgcat atttaatttt gctgaataaa
    31561 ataattgaat aaaatttttt attcagtcca agtaaaagtg acaaaaacat aagagcaaat
    31621 tattcgtaga tatgctacta tgcaaataaa tagaaatgaa aaatgaaaga ggaggagaag
    31681 aattggaaaa gatgatcgaa gtgaaacatc taagtaaaag ctacggcagc aaactttctc
    31741 ccgtcaaagc cttagataat atctcattca cagtagaaga aggcgagttt gttgggatta
    31801 tgggcccaag tggtgcagga aagacaacgc tgatgaatat cttgtccacg atcaatttgc
    31861 ctacgatggg cagtgtctcg atccaaggaa aagaaatcac aaaaatgaaa aacagtgagc
    31921 tcagtgactt tcgtcgaaaa aaactaggat tcatttttca agagttcaat ttgatcgata
    31981 ccctaaatgc aaaagacaat atcctattgc ccctagcagt tgaacggatg actaaagagg
    32041 aaatggaaaa gagagtaaga catgtagctc aattgttgaa tatagaagaa ctgctcaaac
    32101 ggtatcccga tgaactttct gtcggacaga gacaacgaat tgcagctgct agagcacttg
    32161 ttgttcagcc acaagtgatt tttgcagatg aaccaactgg ctctttggac tcgaagtcgg
    32221 ctacagaatt gttgaactac ttgaagacca tgaatcaaaa agaaaaagca acgatactgt
    32281 tagtcaccca tgatccatat acagcaagct actgcgatag aattttattt attaaagatg
    32341 gtgtgatttt ttctgaagtt gtcagaagag ggacaagaag agagttcttt gagaaagtaa
    32401 tagacatgca ggcgacgatt ggcggaggag gaaaaatgaa tgctgtataa actagccgtt
    32461 aaaagttttt ttcggcagtc acgaggctat cttgtttatt ttttcagttt gactttatcc
    32521 gtaatgatct attattcctt cagcgcaatg acctacgatc aatctttagt cagaagagca
    32581 agtcaagata cttcgataga tggtggattg agttttggtg gatttatcat tactgttgtt
    32641 ttgctttttt tcgtattcag tgccaaccga tttttcttga atagaaggca aaaggaaata
    32701 ggcatctatc agctttttgg tatgaataaa ctgcagatat caggtatata tgtgttagaa
    32761 atcatgataa ttggattgtt cgcctgtatt tctgggatat tactcggtat tattttttcc
    32821 aaactctttt caatgatttt ggttcgaatg atggatatgg atcttactag tccatttttt
    32881 atctccattc catcagtagt agatacttta tttgcttttt tcatcatcct tttagcagta
    32941 tctgtatatt caatatggaa aatatggcgc tatccaatga ttcgaagttt tggagaaaaa
    33001 gaacaagcag atagttcaac aatgcgcttc aggacaaggc accgattact cgcagtcatc
    33061 gggttactac ttttaacgag cggatatttt ggagctagtc attttaggga aatcacttca
    33121 tggatgatca gttatcatta tcttgataca ttagttttct tttttccttt tttaattttt
    33181 ttcgtttgtg tggtcggtac ttatttgttt ttctgttata catttcggtt atttttgatc
    33241 atattgagta aatggaagat ttattactat ggtatcaatt ttttgataat gggtaacacg
    33301 caagttcatt tgctcaaaag ttggcgtaca aattctctga ttacagtcat tctcggaatt
    33361 tctttaatga caattggcgg cacggctagt atctctacta ttttatccta ccaggagaaa
    33421 gtttcctccc cgatggacta tcagtttgat gttgagacag agaaaaaaat caagccgatt
    33481 ctagcagagg aagggcagaa gataactaaa gaaatcacct tgcattataa agtcattggg
    33541 agtatctata gtcaaaatct cgaagaacct atgtttcaaa ctgctaatct gatcacggaa
    33601 acacaatatc aagccttcag aaaagttaat ccagatttgc cgaacatttc tttgaaatca
    33661 cctggtcata ctgttttgct tgatcaaatg caaactcttt ttcggaatat ttcattatat
    33721 ggatctagta ttggactgcc agataatcaa gaattgaaga ttcaacaaat gcttccaagt
    33781 gtattaggtg acgaaacaat ggtatatcta agtcctgtac tgattgtaga tgataccgtt
    33841 tttgaaaaag caaaaggtgt ggattatcaa gtagtcaatg tagatgtatc aggtggaaat
    33901 aatgaaaaaa tagatgaacg actgagcaat gacctgtcga taaagtggct caatgctgtt
    33961 tattattctt atgatatcgt aaatggaagt ctacaaggag aaattagcac aaagaaactt
    34021 ccggatgacc aaatgaagga agcacaattt agccaagaaa gctctcgttt gaatttctct
    34081 tccagctatt cgaaattgcg tgcagttaat cggcgaatcg gtatcaatat ttttgtcaca
    34141 ctatttgtcg ggatgatcgt gattattgct actggtagta ttttgacagt ccggcaatta
    34201 gcagaagcag aggaagaaaa agaaaattat cacctattga ctaagctggg gattccacaa
    34261 agaaggatca gacggatgat cttcgagcaa aacgcactta ccttctttcc acccatgata
    34321 ttagggatca ctcacgcagt atttgcaatc aatgtcttca ctcagtatat aaaaactgcc
    34381 gattactggt tggcttatct tgtgagtggc ttattgatcc ttatctatct tttgctttat
    34441 gtcattacga gtcagttata ttgtcggatt atcgaggaat gatatacgga ataagacaag
    34501 ataataaata gaactaaatt ttgaatacca tttatttgga ataagaggtt gtgacacgat
    34561 ttttgtcaca acctctttat tttttatatt ttttaaaagt acgagaaatt ataaagaaaa
    34621 aagcagtgat ttgatgtgct atttttataa aagctatgtg acaaaactgt tagtaaaaaa
    34681 acggtttctg taagctagac acatgtttct ttttgtcaaa attagttaca ctttgtgtga
    34741 ggttatatga ggagggaatc caatgaacaa atggcgttct aatccaaaat tgaattattg
    34801 gggaaaattc atcattcgaa caatttttta tagtgcagta ctgctgttcc tgatttatct
    34861 gtatcactat aaaaatattc aaggaggaac ttttatctat aacgaatttt aggaggaaga
    34921 gtatggaaat caaaacgatt attgaagcaa tcgatgattg gggaataaat gaacctgatc
    34981 gagttgctta tcaggcggat gagacgcata cttttggtga attgaaacaa gcatcagacg
    35041 cacttgctta ttatttagaa aaaaaggtag aaggcgatgg accaattgtc gtttttggca
    35101 atttggagtt tgaaatgatt gtctcatttc taggggttgt caaatctggc catgcctatt
    35161 tgccgattga agagcataca cccaaggagc gtatcctatc gatctttcgg gtggctaaac
    35221 catctatggt catcagtatc ggggactgga tagaagatat tccttcggtt cctgtcataa
    35281 caaaaaaaga atttcaagaa atcaagaaga taccagtagg attttatccc agaaatagcg
    35341 taaaaggtac agaaaatttt tatattattt ttacttctgg tactactgga gaaccaaaag
    35401 gcgtacaaat cagtcatgat aatctagtca gctttgttca atggacgatg gaagactttg
    35461 ggatcaagcc tggtatgcat ttcttagccc aagcgccatt ttcttttgat ttatcggtat
    35521 tttctattta tcctgcatta gtttctggcg gaatgctgaa gccgttaaat aaagcagttg
    35581 ttcaagactt tcgtcaatta ttcgcgacat tgccagcctt gaagttgaat gtgtgggttt
    35641 ctactccatc atttatggat atttgcttga tggaacctac attcaatgga gaaaatgtgc
    35701 cggaattgga catgttttta ttttgcggag aagaattgac gaaaaaaact gcagaaagtc
    35761 tacttgatcg attcccaaat gcacggatat ataatactta cggtccaaca gaagcaactg
    35821 tagctatttc aagtattcag attgaccagc atgtactaga tagttatgac cgactaccga
    35881 ttggctacgt aaaaaaagac acgaaagttt ctattgtcca agaaggtaaa cctgtttcaa
    35941 aaggagaaac gggtgaaatc attattgcag gaccaagcgt atcaaaaggt tacctgaaca
    36001 atcccgaaaa gacagctgct gcgttctttt gtcaaaatga cttagcaagt taccatacag
    36061 gggatgcagg tagactagat gaatatgggc ttttatttta tgaaggacgt atggatcagc
    36121 aggtcaaatt acatggatac cggattgaac tcggggatat cgaacattat ctgctccaag
    36181 acaaccgaat caaacaagct gtcgtcgtac caaaatacca aggaacaaaa gtacagcagc
    36241 tggttgcttt tgtcgtatta gaaaacaaaa cgaaacagcc ggattttcag ttgactaaat
    36301 ctatcaaaga acaattacta gaagttgtaa tggattatat gattcctcag aagttccatt
    36361 atgtcgatct gctgcctcag acagttaatg gaaaaatcga tcgaaaaaaa ttaacagctg
    36421 aggtgaatcc tgaatgatgt tacctcatat gatcccttat gcagagccgt tttatttttt
    36481 tcttttagcc attgcgcttg taccgatcgt tctatcttta ttgatatggg aaaaaagatt
    36541 accagtttat caatcagcag tgacactttt ctttttattt gtcagctttg gaggtaatta
    36601 ttggcaacag ggagcggcgc tgattgggta tgttttgtgg cagacgatac ttgtatgggg
    36661 ctattttgtt tatcggcaaa ggaaaaatca gacaggatgg ttttttgccg ccgttatttt
    36721 agcgataatt ccactgtttt taacgaaagt gactcctttt tttgaacatg gaaaagcatc
    36781 gttacttggt tttttaggaa tctcttattt aacctttaaa tctgtacaaa tgatcatgga
    36841 aataagagat ggcatgatca aaacatataa gccatttcgt tatatccagt ttctgctgtt
    36901 ctttccaaca atctcttctg gaccaatcga tcgctatagg agatttgaaa aagatcagct
    36961 ccatccgcct gttcgtgata aatatgttga tcttttggga aaaggcgtcc aaaatatttt
    37021 tgtcggtttt ttatataaat tcattatcgg ttattatttt ggtcaagtac tgcttcctgt
    37081 agtgggacgc attgccttac agcatggtgg tatttcatgg gcattagtag gttatatgta
    37141 cgtgtacagt atgtatttgt tttttgattt tgctggctat agcttatttg cagtcggtac
    37201 aagctacatg atgggatatc agacaccaat caatttcaat aaaccttttt tatcatggaa
    37261 tatcaaagaa ttctggaatc gttggcatat gacgctgtcc ttttggttcc gtgattatgt
    37321 atatatgcga ttgatgttct tcatgatgaa gaaaaaattt tttaaaagca agatcgtaat
    37381 gtcaaatatc ggttattttg ctttgtttct tctaatgggt atatggcatg gattgacgtg
    37441 gtactacatc gtatatggac tgtatcatgc ttgtctgatc tgtctgacag atgcatggat
    37501 ccgatttaag aagaagcata aagagcagtt gccttctaac aaatttacgc atggcttagc
    37561 cgtatttttg acatttcaag cagtatgttt tagcttcctg attttttcag gatttttaga
    37621 taaacttttt tttagctaac gaatagtgac atattaattt atataacaaa ggagaaaagg
    37681 aaaatggaag aacaaatttt atcaatctta gaagaaataa caggaacaga tgaagtaaga
    37741 aaaaatcgtg atattgatct atttgatgaa gggttgcttg attctttagc atctgttcaa
    37801 ttattagtcg agctagacgg agagctggat atccaagtcc ctgtatctga attcgagcga
    37861 gaggattgga gcacaccgaa taaaatcatt caacaagcaa ctgcattgaa gggttgagta
    37921 tgaaaaagaa actttttgct attttcggcc cggttgtttt agctgttggc ttactgtttg
    37981 tgttttttac ctcctcctat caagtagata tctctaatcc agaaacgatt agacgtgcct
    38041 cttcttccat gtcagtgaac gtgttgaaag gagatgcgat aaaaaattat gcattatctg
    38101 aaaaacaata tattcctttt tttggctcaa gtgaattaag cagaatcagt ccatttcatc
    38161 cgtctgttct ggcagaaaaa taccagcgaa actatcgtcc atttttgctg ggagcacccg
    38221 ggacacaatc gttaagccaa tatatgatga tgcgttctgc aggagatgct atgaaaaaca
    38281 agaaagtcgt ctttattatt tctcctcagt ggttcgttaa aaatggtgta aaaactgatt
    38341 actttaatac gtattattct gaacttcaaa catacgactg gttgttttca atgaagaaag
    38401 taacgcctgc tgatcgctat ttagctagaa gattattaac tttctcaaaa gtaaaagaga
    38461 atgatacgtt gacggcgatt ctgcaaacga tcaaaaaagg gaagctacct ttacctgaat
    38521 ctttgaatca gctgcgcagc caatggaaca tgctgaagag agaggatgaa gtattcagca
    38581 atattgggtt gaaagatcga cagcaaaaaa tcgatcatga gtcaaaacga ttaccaaaac
    38641 aataccaaga aacagaatta tccattttag caaatcaaat tggagaacga gaaacgacga
    38701 ataatccatt cgggttgaaa aacgattttt atacgcatcg tatccgagcc cacgagccag
    38761 agctcaaaca gtcacaaaaa aattgggatt atcgtttttc gccagaattt tctgattttc
    38821 agctggtctt ggatcaatta gcaaaaaacc ataacgaagt attgttcatc atcccaccgg
    38881 ttaacgagaa atggagcgat tataccggac tatctcaaga aatgttacaa ggatttgcca
    38941 aaaagatcaa atttcaatta aatagccaag gattcaatcg aatagctgat tttgtcaatc
    39001 aagcgggtac caactatttt atggaagaca cgatccatct aggctggaaa ggctggttgg
    39061 ctgcagatca acagatcaga ccgtttttag aagaaaatca tattactgct agtaagtatc
    39121 atcttgacga tgcttttttt agcaaatctt ggcaacatca aataccagat aaactacaat
    39181 taaaataaag aaaacaggcg agtgttgctc gcttgttttc tttatttttg aatattctga
    39241 ataaaagata aaaataccaa gtttgcctga acaaaaatca taagcaatgg tataatcaca
    39301 atatgaatat agaaatgggg gaaaacattt ggatacaaaa gaagaacagc tacttattga
    39361 tttaacttct gcaaaaggag tacctggaaa tgaagaagag gtaagagaag tattcagaga
    39421 atatgccaaa ccattcgctg atgatatttt ttatgacggt ttaggcagtg tgattgccaa
    39481 acatggtgct ggcggaggcc ccaaggtctt tatttctgga catatggacg aagttggttt
    39541 tatggtaaca aaaataaccg aaaaaggatt cttggaattc caaactcttg gaggttggtg
    39601 ggggcaagtc atgcttgctc agcaagttga aatcaaaaca cgtgaaggga aaatcgtcca
    39661 cggtgtgatt ggttccaaac caccacatgt tttaacacct caagtaagaa ataaacctta
    39721 tgaaataaag gatatgttca ttgatattgg cgcatcaagc caagaagaag ccaaagagtg
    39781 gggcatacgt cctggagata tggtcactcc atatattgaa tacaaacgaa tgaatggcag
    39841 taaatattta ctggcaaaag catgggataa tcgtatcggg acagctgttt ctctgcgagt
    39901 attagagaat ctatctaaag aagcacaccc aaatgtttta ttcgcaggaa gcgatgtgca
    39961 agaagaagta ggcttgcgag gtgctcgaac aagtacacac cttgtaaatc ccgatattgc
    40021 ttttgcttta gatactggta cagcaggaga cactccagga atgacaccaa aagaagcaga
    40081 ttcgatttta ggaaaaggcc cacagatttt gatttttgat gcatctatga tcccgcataa
    40141 aaaattattg aattttgtta ttgatgtagc agaggaaatg gatattccat tccaatatac
    40201 agtgatttct ggcggaggaa ctgatgcagg acaaatgcat ttgacaagag atggtgttcc
    40261 gtctgtagcc attaccgttc ctgttcgtta tctgcattcc catacatcag tgattcatga
    40321 agatgattat ttgaatacag tgaaactagt cacagaatta gtgaaacggc ttgatagtga
    40381 aacagttgaa aaaattcgga gttatgaata atgaataata gaatacctat ttctgaatat
    40441 cggaaaaaag cacgagtttc tttggaaaat caatggggaa tcaatacttg gcttgttttc
    40501 ttatctataa tatttacgag cgtcatccag aacttttttg acggtttcag tccttttccg
    40561 gaagaaagca tgcagcagca cttattgagt tttctagtcg ataatttact tttattcgca
    40621 ttcaactatg cattgtttta cattgcgcta gtagttgtac ggggtggcag accaaaaagt
    40681 aaccttttat tttcagtttt ccaaagaaag tattttggtt cgatacttgg catcaatttg
    40741 atcaattcag tcatcaactg gctactcaac agtttaatct ttctgcccat gtttttaatc
    40801 agcggagtga gtacttatat gcagctgctt ttcagttcaa atgcggtacc tacgggtgtc
    40861 atgccagaag attttcttga tatcagcttt gttttatcga tgatagggat gatttttgtt
    40921 tcagcactca tcatgagtat tgttggcggt ttattccaat ttgctgcatg gacaaagttc
    40981 gactatcctg atttgtcggt tattcaatgt ttgaaatatg gctggttttt attgaaagat
    41041 agaattggta cgtatatttt gttgcagctg tcttttatcg gctggtattt gctaggagca
    41101 atcgctttgt tcatcggtct tttgtgggtc attgtttatg tgaatgtgtc agttgcagaa
    41161 ttttatgaac aagcacggat tgaaaaagga ggtccagacg cctattttac ttagcatgta
    41221 agcagtttat tcatcaaaaa aataactttt gtggtaggat aaatctttga gggggaatgt
    41281 acgatgaaat taactgtttt aggatgctta ggagcgtatc cttataaaaa tcaagggaca
    41341 acaggttatt tgcttcaatc tgacgggttc aatttgttga tcgatgcagg tagtgctacg
    41401 ctgatcaaat tacaagaaca ccttgatcca cttgatctag atgctgtgat ccttagccat
    41461 tatcatcatg atcatattgc tgaccttggt gtattacagt attactggca acttcatcct
    41521 aagagaaatc cggagacatt gctttcaatt tatggacaca caaaagattc cattcacttt
    41581 gaagaattga cgttggaaaa tgtctcaaaa ggacatcctt attttgaagc tgaagaatta
    41641 aaaattggtc cttttctgat tacttttatg gaaacaatcc atccagtagt atgttacgcc
    41701 atgcgtatcg tagaagaaac aacggggaaa gtattcgtat ttacaggtga ttcaggttat
    41761 ttggaaagtt tcgctgattt tgcgaaagat gctgatttgt ttctagcaga tacctatctt
    41821 tttgaaggaa atgaacatca tcatgcccat ttcacttcaa aagaagctgg agaacttgcg
    41881 aaatcagcta acgtaaagaa acttgtactt acacacctac ctcagtttgg tgatttagaa
    41941 caattgaaaa atgaagcgca aaaggcagca ggcaaaggga ttgatataga acttgctgca
    42001 gttgacaaag tgtttaacat ttaggaggaa gaacatgatt tttgtaccaa atgaaaacca
    42061 cgacccaaga atcaatttag caattgaaca atatttgctg caagaaatgc ctttagatga
    42121 acctattttg ctcttttata tcaatgaacc ttcaatcatc atcggcagaa accaaaatac
    42181 gcttgaagaa atcaatcgag attatgtgga agaaaatggc attcatgttg tccgtcgctt
    42241 aagtggaggc ggagctgtct accatgattt tggaaacctg aactttagtt ttatcatgcc
    42301 tgatgatggg gattcattcc gtgactttgc gaaagtaaca cagccaatca tccaagcact
    42361 tcatgaattg ggtgttgctg gggcggaatt gaaaggccga aatgatctag tcattgatgg
    42421 catgaaattt tctggcaacg cgatgtatgc taccaacggt cggatgtttg ctcatggaac
    42481 aattatgttc gactctgata tcaatgaagt agtcaatgca ttaaaagtaa aaaaagacaa
    42541 aatcgaatca aaaggaatca aatcgatccg ttctcgtgta acgaatatca aaccatttct
    42601 tccagaagaa aagcaaaaaa tgacaacaga agaatttcgt caagaaatct tgttgaaaat
    42661 tttcggcgta gagtcagtag aagaagtaaa aacgtacgaa ttgactgatg aagattggaa
    42721 aaaaatcaat cgaatctctg aggaatatta tcgaaattgg gattggaatt acgggaagtc
    42781 tccagatttc aatttctctc gccaaaaacg tttttctatc ggttcgattg aagtccattt
    42841 gaatgtctca gaaggaatga tcaaagaagc aaaaatcttt ggcgatttct tcggactagg
    42901 agatatctct gacgtggaaa aaaaattggt tggacagaaa tatgataaag catctctatc
    42961 aaaaatagtc agccaaattg atgtgaagaa atattttggt gcgatagaac cagaagaatt
    43021 gcttacctta ctttattgaa aataaaaaat agcggaatcc acatatatgg attccgctat
    43081 tttttataca ggattcgggt aatttcgaga gagttgaaca gtgatccgat caatgaaagt
    43141 atctttttcc tgcgttagtg ttcctgtgta acgatccgct acgtctttca tcatcattaa
    43201 gtcaaaacgt tcctgatcat ctaattcttc tgtgatgcct cgactaaata ggtttgtatc
    43261 gtaatgtgca ctatgttcgc aacaaatgac gaaatgttcc tcttgttcgt gtatcgacaa
    43321 ataaattttt cgatacagtg gattttcgat ggcatgagtt tctctaaagg aatgctctaa
    43381 agcgtggatc aaaagttgag ccagatcgtc atcgttgata ggaagttctt cgggaagatc
    43441 tgcttggaag gttatctgaa tattccgttt ggcagctaac ttctggtaac gcgctaagat
    43501 aagatttgtc acctggtggt tgcaaaaaga acacagcatt tcgaaatttt ttgcttcttc
    43561 taatatcttt tccacatagt ttttagcttt tgtttggtcc gtatgcatta aatcttgcag
    43621 attctccatc tcttggacaa attccaggcg aagtgtattt acttcttgga tatgctgtcg
    43681 aagctgctca taatgttgct ccaacaaatc atttttgatt tttaagaaat ctacttgtct
    43741 ccgatctttg tcggtattta agatgtgatc gattagattg tagctgataa taacgattag
    43801 aatgataata tataaaatcg tagaccaatt gatcgttgtt tgatagatac ctaatgccgt
    43861 ttggatgtaa atcaagcaat ggataatggc taccaagacg atccaaggtg tacaagctac
    43921 ataaaaacgg ttattcttat atgcttcgcc aatgcatgca agtgaagtga ctaaagtgct
    43981 tataacacta gcaatattag caatcggcat taattcagga agttctgcag gagtaaaagt
    44041 tgcataaagc agaacgccga taaaaatcaa aagttggaac gtacaaaaaa ttccgtacca
    44101 ttttttatag taaaacattc ggctccaata gtagcagatg atgaaagctg acgttaagat
    44161 aataaataaa tgagataaaa aagaatgaac gaatggttca aaaagaatac cacttaaaat
    44221 atcttctgat acagcttcaa atcctaatgc caaagcaaag caacttaaga gaataagtga
    44281 gtattcaaga cgtttttgtt tatacatcaa gtagatcgta tagaaaaagg tccctatagc
    44341 gataaaaaca gcgattatca tcgtcaatac ttgcggcact gattgtgaat aaataaaact
    44401 aatcaatggt ccagttgttc ctagataaac tttcggtggc agtccagcat agttctgata
    44461 aggagatgag actgtaaggg tcaatttttt tccgatataa tttgctggta aatctatcac
    44521 agccaatgag agtccgggat tgctttcttc ctgataatct ttccgttggt ataaaatctt
    44581 ttcgcctaga tatacagtaa tccattgatg gtttcctttg attagtaaat ttgcttcgtc
    44641 caacggtttt tcaagtgtcg tttccataat gaatgtttcg tctttatcca cggtaggaat
    44701 cgtacgagtg tacttgctgt caaacttaaa atcaggatca tcttttgtat agaaaaccca
    44761 cgaatctttt atttctttca tttgattgct gtccgcagga gaaaaaataa aataaaagaa
    44821 ataagcaatt cctgttaaaa ataacaatga taatataatg attatgctcc actttatacg
    44881 taaataaaaa gattcttttt tattatatat gttattaaaa aaagacataa aggtattcct
    44941 ttctaaaact tgtaatgtta gggtaaacgt tttttaaact aacgtcaatt atttataata
    45001 aaaaatgaga taaagatgag gaaatagaat tagaaacata taattattaa agggaaacat
    45061 ttttacagct ataatttgaa aaaaatttat cgttattata tgattaagaa ggattgaata
    45121 aagaaagaag ttgaaaacga tacctgatta caaaaaaata acttttataa gttctataac
    45181 gttgattata tttttaattc ttattcttct cgtcgtcttg gttccccgaa tttttggttt
    45241 gactccttat attattgagg acaaagaaat gagtccatat tatgcacaag gaaatctgat
    45301 atatgtacga ccgaaaaata caaatgagat tttagtcgga gatattatta catattatga
    45361 aaattcagga aaaaaaatct cgacgcgtcg cgtaatagcc gttgaaaaca accacgaaga
    45421 tttttataca aaagctgata caaagactta tattgagcct ggagtagtaa aaaagagaaa
    45481 tattatcggt agtcccatct ttcagattcc atatattgga ctagtccttt caaaaacggc
    45541 atttgcatgg attcaggggt tattctttac gattgctttt tgcctaacag gaatcacagc
    45601 atggaatacc tttgtagagt tgaaaaagaa gcggagacga acacagaaat ttttgtaaga
    45661 atgttaaaaa cataaatgta ttccagagat ccaacgactc ttctgaacat gaaacacgag
    45721 aaaacactca tgcagcttgt agatttagtc agttgttcgt ataatgtgtg aagaggagga
    45781 gtagtatgcg caatgaaatg atgcatcgtc ctgtagtcaa agaagaacta ttggatttta
    45841 tgcgtaccca gcaaaaaaaa ttaccgggtg agctaggcaa actggaagaa gaagctcatg
    45901 tagcagaagt accagttatt ccccatgaaa cagttgtatt tttgaaattt ttacttggac
    45961 agcttcagcc tgaacgaatc ttggagatcg gagcagctat cggtttttca tcaagtgtca
    46021 tggccacgac aatcggagaa aacgctcatg ttacgacaat tgatcgtttc gatgtcatga
    46081 tcgagaaagc taaaaaaaac tatgaacgtc taggactgac agataaagtg actttgcttg
    46141 aaggtcaagc agcagatatc ttgccggagt tgtctggtcc gtatgacttt atttttatgg
    46201 atagtgcaaa atcaaaatat attgaatttt tacctgaatg cttacgtttg ttaagaaaag
    46261 gtggcgtttt gatggtagat gacatcttcc aaggaggaac gatcttgctc ccagatgaag
    46321 agattccccg tggcaaacga gctatccatc gtaaattaaa tgaatttctt cgtgttgtga
    46381 tggatcatcc cgatttgaca tcgacaatct tgccattagg tgatggtgtg atcttaatga
    46441 caaaggaatc agaaacaatc gagttgtgat acatgattat ggcagcctgt agataatcga
    46501 ttgaatcgag ggctcaattc gaacttgatg taaaagtttc tatatttgta agtcaattaa
    46561 aggatacagg aaatctaaat aaacaggagt agcatgaagt caatgtagct cctgttttca
    46621 attgataacc ttagaacagc tgaaggagaa tgtctcgttt taaaagattg atttagtagt
    46681 tgggccaaaa taattcatta tttgctaatt aaataactat ttcgtattga cagaagcaat
    46741 ttaattatct ataattattg atgttccgct gattcttgtc atagtagctc agctggatag
    46801 agcatccgcc ttctaagcgg acggtcgggg gttcgaatcc ctcctgtgac gctaaaaaac
    46861 cgttagaaac tgaggtttct aacggttttt ctttgttttt tgtgccaata ttgtttgttt
    46921 tcaaaggcaa ataaaataaa aaaacatcct tatcgagtac aattataagg agcaaaaaaa
    46981 taaaactcat cagaaaagag gaagaagata tgacgatact atctagaatt ttagtgactc
    47041 tagttgcaat tgaatttttt tatatcatgt atatcgaaac ggtgagaact gattctgaca
    47101 cgacaagtag ggtttttaaa atgagtaaag aagagttaag cagaaaatct gttcaaacat
    47161 tgtttaagaa ccaaggagtg tacaatggac tattaggagt gggactactt tatggagctt
    47221 atctttcttc cgcatctaaa gaaatcacaa gtatgctatt aatatctatt ttttttgttg
    47281 cattatatgg cagtttgact agcgatcgtt ggattttact caaacaaggt gggctagctc
    47341 tgttggcatt gctttctatt ttcttataaa tatagaaaaa ataagaattt atctaacagg
    47401 aaactttgtg aaaaatatta ctttatctca tgaaatatga tattctaatg aatggtattt
    47461 tagaaaattt catgaaaaag gagttagata ggtgttcaaa aattatgttt cctcactaaa
    47521 aaaagaattt tcaggttatg atctttcaag atttcagaaa gatcttttgg ctgggatcac
    47581 agtagctgct gtagcattgc ctttagcctt ggcttttggg gtatcaagcg gagctgatgc
    47641 ggccgcaggt ttgattacgg ctatcattgc cggtatcgtt attggcggtt tatcaggcgg
    47701 tttttatcaa atatcaggtc cgacaggtgc gatggcagct atcttgatgt ctattgcggc
    47761 aacaaaggga atgcaaggtg tattgcttgc tactttttta gcgggtctat ttttacttgc
    47821 agcaggactt ttccgattag gaacactgac ttcttttatc ccagcacccg taattacggg
    47881 gtttacttca ggtattgcta tcattattgc attaggtcag atcgataatt tatttggtgt
    47941 tcactcggaa ggtacaaacg ttattgaaaa actaagtagt tatcaaacga taggatttcc
    48001 gataaacaca gcctctttga tcatgggagg gctagtcatt ttagggatgt ttatctatcc
    48061 aaaaaaatgg gcgaaacgaa tgcctagttc gctgctggct atcattttgg caacagcagt
    48121 tatgctattg ttccatttac cagtagcaac tgtgggtaag attccacaaa cattgatcag
    48181 tagtaatcgt ttgaatatgg gagattttag cttttctgcc ttgcaacaag tggcagttcc
    48241 agctattagc attgctttat taggaatgat tgaaagcttg ctatgtggtg cttcggctgg
    48301 ccgaatggca aataagccct tagatagcaa tcaagaatta atggcgcaag gaattggaaa
    48361 tctgttgctt cctttctttg gcgggattcc tgctactgcg gccatcgccc gtacaagcgt
    48421 ggccatcaaa tctggtgcac aaactagatt agctggtatg atacatgcag ttgtcttact
    48481 gctctccatg cttgtttttg cgccagtgat gtcacagatt ccaatgccag cacttgcagg
    48541 tgttttgatt gtgacggctt ggcgtatgaa tgaatgggaa acgatcaagg agctatttac
    48601 taataaatat tggtcagcag ttctattgtt tatccttacg atgatttgta cagttatctt
    48661 tgatttaagt attgctatcg tgatcggggt gatcggcggc tgtgtatttt ttgttgtcaa
    48721 aagcgctgct atcacgattt cattagaaac gatcgattgg caaagaatgg ggctgccaga
    48781 aacggaaaag ctgaataact ggacagtagt gtatattagt ggtccactgt tttttatgtc
    48841 ttcagaaaaa ctcaaaaata aattggaaga actaaatcat cacgatggtg tcattttctc
    48901 catgcgaggc gtaccaagta ttgatttgac ggcacgtgca gtttttgatg aatttcaaga
    48961 aaaagcagag caaagaggac aaacgattat ctatacttcc ttgcagccag aagtagaaaa
    49021 acaactttct cacttgtggg aacatacatt cacgcatcaa catcaaacag ttgctcatgc
    49081 gttgacagct ttacatgcga aatatactgc aattagccat taaaacaatg aaaaagaaca
    49141 gacaagactg taaactgaag attttatggt cttgtctgtt ctttttaata ttgctgtaaa
    49201 agtgaagcta gtacttgcca gtcttgatgg ataacaggta caagggagcg attgtagaaa
    49261 atagctgctg gatgaaaaag tggaagaatt cgatagtttt tttctgacca ttcgtaggca
    49321 tccttttgtt ctgttaattg taagacaggc cctgtataga gttgaccatg atttttggta
    49381 accgtatatt ctttgccaag taaacgttga agaccaatgt ttccaagtgt cgcaattaat
    49441 tttggctgga ccttcatcag ttcgtaatcc aatattggtg catgcgcaaa tacttcggaa
    49501 cgagtaggtg tccgatttgg ataaacagtt tctgactgtt gtgtccgttt attgatccga
    49561 tgagtcacgc gatacgggcg gctccttaca gcactggtga tatacacctc ttctcgtgtt
    49621 aagccagttg aagaaagtgc ctgcatcaat tcttttccag cttgaccgct aaaaggaaca
    49681 tgcgtatcaa tctctgtttt ccctggggct tctcccacga gcatcagttt aggatgaaga
    49741 ggtccttgcc cagcgataaa tccttctaca ggacggccgt tcattcttaa agaagcttct
    49801 tgtatcagtt cttttgggta ttccatttat gctcctcctt tttcttttat tataagaata
    49861 aaagaaacac aatgcgacta ttttatttgg tagaactgtg aggatagaaa agcagaagta
    49921 ttgcttgaat ttttgtttta ctttttgatt ttaaatttag ttgactaacg gttgctgttc
    49981 tggtaagcta gtactgttgt aattgtggac tccacagcta caaccgctca gaacaagtaa
    50041 taagcacagc atatgctgtc acttcggatg gcgagtctaa gtatatcaga aggaggtgct
    50101 ttatagcatg tacgcaatta ttaaaactgg tggtaaacaa gtaaaagttg aagtgggaca
    50161 agcaatctat gttgaaaaac ttgacgttga agcaggagaa aaagttgttt ttgacgaagt
    50221 tatcttagta ggcggagaat ctacaaaagt aggcgctcca actgttgcag gtgcaactgt
    50281 tgaaggtact gttgaaaaac acggcaaaca aaagaaagtc gttactttca aatacaaacc
    50341 taaaaaacat tcacaccgta aacaaggtca tcgtcaacca tatacaaaag tggttatcga
    50401 cgcgatcaac gcttaatagc gtttgagaaa gaaggcctat atatgatcaa aggaacattt
    50461 aagcgaaatg acgcaggtca gatcgtttca ttcactttga ccgggcatgc tgatgctggt
    50521 ccttatggaa gtgatatcgt ttgtgcgggt gtttctgcgt tagcgatcag cactgtcaac
    50581 gggattgcct cattagccgg ttttgagccg attgttgaaa tgaatgaaga agaaggcggt
    50641 tatctttata ccgaagtcac ttcaggaatg acacaagaac aaaacaatat tgctgagatt
    50701 ctattagaaa atcttttatt aggattacaa tcgatagaag ctgaaaactc agaatatatc
    50761 caaattaaaa caattaatga taaataggag gtgcagacca tgttgttatc tatgaatcta
    50821 caattatttg cccacaaaaa aggtggcggt tctacatcta acggccgtga ctcagaatca
    50881 aaacgtctag gtgctaaacg tgctgatgga caaacagtta caggtggatc aattctttac
    50941 cgtcaacgcg gtacaaaaat ctatccaggt gtaaacgtgg gtatcggtgg agacgacact
    51001 ttatttgcaa aagttgatgg cgtagtacgt ttcgaacgta aaggccgtaa caaaaaacaa
    51061 gtgtctgttt atccagtagc tcaagaagct taattgaatc ttatgattaa agaggttgtg
    51121 acaagacttt tgtcacagcc ttttttgttt ataaaaataa aagttgtagt ggaggctacg
    51181 aagtttactt tgtcttttta cacttaatat ttttttgcca atttggaatc aggatgtttt
    51241 gataactatc gttttcaatt gatggaagct atattgtttg ctatcagtga aataattata
    51301 tggtaaacag tgttcttttt cttgtttcca ttataaaaat cgtatcggga tttttcgttc
    51361 ttttaaattg aacatttcta tctatccgcg tgttatcaac aaaacaaaat caggtaaata
    51421 gttgacgaaa cagaaataaa tacatagtat tatgttttga agcctaagta caatagtcta
    51481 tagaaaagga gcgataccat tggcaaaacg cattgaccga atttatatgt atgtcaaaga
    51541 aaaaaccgct catttaacaa aggcagaata tgatcaagga gtaacaacac aggaaattgc
    51601 tgaagtgtta gggattcagc gaacaaatag cagtaaagat ctgaatcagc tagtgcgcga
    51661 aggaaaactg ataaagacag acggacgacc agtacgttac atatatcaaa cactcgttac
    51721 gcacgtaaag ccagttacaa aacatgtagt cagttacaaa gaaacgccgg tctctgaaga
    51781 aaaaaataca ctccaaatag atactaagga tatctttgca aaaatcattg gagctaacgg
    51841 cagcatgaaa aattcagtgg aacaggcaaa agctgcgatt ttgtatccgc caagaggatt
    51901 aaattgtttg attacaggac ctaccggatc aggaaagact tattttgcac acgcgatgta
    51961 tcattttgca aaagcgcatc atgtaataga tgaggaaacg gaactgattg ttttcaattg
    52021 tgctgattat gccaataacc ctgagctttt gatgagtcat ttatttgggt acgcaaaagg
    52081 tgcttttaca ggcgcagatg aagaaaaaat ggggattatc gatcaagcgg atggcggtat
    52141 gctgtttttg gatgagattc accggttgcc gcctgaaggt caagaaatga tcttttactt
    52201 tatggatcat ggaacttaca gtcgtcttgg ggaaacaacg aaatcccatg aagcaaatgt
    52261 acggatcgta ggtgcgacca cagaagaccc aggttcttct ctattagaaa cttttgttcg
    52321 gcgtatccca atcaatatca aacttccggc ttttgaaaaa cgtcctgcaa atgagaaagt
    52381 cgatttagta aaaatcatga ttgcccacga agcaaaccgc acgcagcgaa agatttcttt
    52441 gacagaagat gtagcaaaag cattgatcgg cagtgtgacc tatggaaata taggacaatt
    52501 gaagtcaaat atccagttag tatgtgcacg aggatttcta aatcatatgc aatcacccga
    52561 aatctcgatc acaatcgatg atttgacaga agggattcgt agcggattga ttcaattagc
    52621 aagcaaccgc agtgcaatgg cagagctttc taaaatattg gagccaaaga taactgtagc
    52681 accaaatgat acgatcatga aaatccaatc ggactcctat gagttacctt ataatctata
    52741 tgatatcatc ggagataaag cagctttact gaagtcggat ggtttggatc aagaagcaat
    52801 caatcatttc atttctacag atatcaacat ccatctgaag tctttctata aagaccatgg
    52861 tttttctttt aatgcagaca gcaaattagc agaatttgta gatccgaaaa tcatagaagt
    52921 gaccaatcag atttatgtaa tggtcaaaag atctttacca tatgagtttc agcaaaactt
    52981 tatttatgct atgagcttgc atatcagttc ttttttgaaa aaaatcaact taggagaaga
    53041 acggcatacg aatgataaca tccgtgagat ggcttttgat tttccagatg aatatgctgt
    53101 tgcgaaagaa gtgcgtagtt atatcgaaac gtattttcaa gtggagattc cagaaagtga
    53161 agactattac ttgaccgttc tactcgtctc gttacgggca aatcaagcaa gcggcagaat
    53221 cggtgtagtc gttgcagctc acggaaacag cacagcaagc agtatggtcc aagttgtcca
    53281 acagttgtta gatgcagaga acgtacgagc agtagacatg cctttggata tggatccgaa
    53341 aacagcattg gaccgaattg aacgcaatgt acaagaagta gatgaaggaa gcggggtcat
    53401 actcttagta gatatggggt cccttgcgtc gtttaattcg caaattcaaa gagacactgg
    53461 aattccagta cgtactgttg atatggtaac aacttctcta gttttggaaa ctgtacgaaa
    53521 ggtctcagta ttagggacag atctagatat gttgcatgaa tcattgaaaa attttcgagg
    53581 ttacgcagaa atatcttctg tagaatctgt tcaggaagca aaacctattc ataagaaaaa
    53641 agctgttttg gctatctgcg catctggcga aggtacagca caaagaatca aagaattgat
    53701 cgaacgagcg gtaaacaagc gtcaagaaac agaattagaa gttctagctt tgtcgattgt
    53761 ggaagtgaaa gaagaactgc cagttatcca aaaagaatat cagatcatag ctactactgg
    53821 gattactgat ttgaatatcc aagccccatt tattccttta gaacgtttta ttgatcaaaa
    53881 tatcgaagtc attttggatc agttgctgat ggaatcagaa ttggaggaaa ctgaattcat
    53941 ctccctagat gaagagtcag ctaaaaatac gtgtgttgag tttatctctg ataacttcat
    54001 ctttattaat ggtagtaagt taatcgatcc aatgtggcaa ttttctacac aaatctctca
    54061 gacgacggga atcggcgatg aagaatatgg attcaaaatc aacctagtga tgcacacagc
    54121 aggaatgatc gaacgtatta ttcggaatga accattgact gtggaagaaa acgaattgac
    54181 aaatacaacg aatgatcctc tttatagcca attggcagcc tcagttgttc tgttggaaga
    54241 tcagataaaa gtaaaagtgc cgatagaaga aatgtattat ctgctacgtc tagtccataa
    54301 tcagttagat aaaaaagaat acactgtgcc atgaatacac aaaaacgata cacttttata
    54361 gtgtatcgtt tttgtgtatt ggttaagaat cctttaaatt caattagtgt atcgtaaaaa
    54421 agttggcacg ctttttgctt atagtagtgt gtgctaggag gtggttgttc acacagaaat
    54481 gataggggtt tctgtgaagt agtgaggaag aaggagtttt gcagatggat attcgattgg
    54541 cacgtataga tgatcgttta atccacgggc aagtcgcaac agtttggtcg aagcaaacag
    54601 gtgttgaacg tatcattgtg atcagcgatg tagtcgctca agataaattg agaaagtttc
    54661 tactaaaaga agctgcaccg ccggggatca cagcaaatgt aataaccgtg cagaaaatga
    54721 ttcaaatcta tcatagtgga ttattatcac gagcaaaagt gatgctttta ttcaccaatc
    54781 cccaagatgt tgaagcggtt gtccgctgcg gtgtacagtt gaagtcgttg aatatcggcg
    54841 gtatgagttt tactgaaggc aaatcaatga ttaccaattt tgtttcagta aatcaacaag
    54901 atattgattc ctttcgttac ttgaacagtc aagaagtgga gttagagatt cgaaaagtac
    54961 ctgctgacag gaaaatcctt ttgatggaac tactagcgaa aatcaaaaag gtgtagcaag
    55021 aatggttgtt ggaaaaggtc aaataattct gttgtaatgg ttagcaatta ttaatatttt
    55081 tgaatttgtt acaacaaaag taaataggag gtataaatat ggtagggatt atcctagcaa
    55141 gtcatggaca atttgctgaa ggaatcttac aatccggttc aatgatcttt ggcgaacaag
    55201 aaaacgtcaa agccgtgatt ttgaaaccaa gtgaaggtcc agatgacttg agagccaaat
    55261 tggaagaagc ggttgcttct tttgataatc aagacgaagt gttattctta gttgatttat
    55321 ggggcggaac accgttcaat caatcaaaca cattattcga agagcacaaa gataaatggg
    55381 caatcgtcag cggtttgaat ttaccaatgt tgattgaagc ctacgcatca cgcttttcaa
    55441 tggaatctgc tcatgaaatt gcagcacaca tcattgaaac agcaaaagac ggtgtaaaag
    55501 taaaaccgga agaattagaa ccggcagaag caccgaaagc agcagttgaa gacgcacaac
    55561 ctaaaggagc acttccagaa ggaactgtgg tcggagacgg aaaaatcaaa tatgtattag
    55621 cacgtgttga ctcacgtctg ttacatggac aagttgcgac agcttggaca aaagcagtgc
    55681 aaccaaaccg tattatcgtt gtttcagatg cagtatcaaa ggatgatctg cgtaagagac
    55741 taatcgaaca agctgctcct ccaggtgtaa aagcaaatgt tattcctatt agcaaaatga
    55801 tcgaagtagc aaaagaccct cgttttggaa ataccaaagc attgctttta tttgaaaatc
    55861 ctgaagacgt attgacagct gttgaaggtg gcgtagacat caaagaatta aatgttgggt
    55921 caatggcgca ttcggtcggt aaagtcgttg taagtaaagt attgtctatg ggacaagaag
    55981 acgttgaagc gtttgagaag cttgaacaaa aaggtgtgaa attcgatgta cgtaaagtac
    56041 ctaatgattc acgcgataac atggatgata ttttgaaaaa agcaaaagct gaattagcaa
    56101 aagcgtaata aataatatta aaatttatag gaggcttatc atgtctatta tttcaataat
    56161 tttagtcgta ttagttgcct ttctagctgg tatggaaggt attcttgatg aatttcaatt
    56221 tcatcaacca ctagtggcat gtacattgat cggtttagtt accggtaacc tcgaagctgg
    56281 tatcgtactt ggcggtacgc ttcagatgat cgctttaggt tgggcaaata tcggtgccgc
    56341 tgtagcacca gatgctgcat tagcatcagt agcatcagca attattttag ttttaggtgg
    56401 acaaggtgtt aaaggtgttc cttctgcgat cgcgattgcc gttcctttgg ctgttgcagg
    56461 gttgttctta acaatgatcg tacgtacaat cgctgttcca atcgttcatt tgatggatgc
    56521 ggctgctgaa gaaggaaaca tccgtaaggt tgaaatgtgg cacatcattg ctgtatgttt
    56581 acaaggggtt cgtatcgcgg tcccagcagc agcgttgcta tttatcccag cacaaactgt
    56641 gcaatcattc cttgaatcaa tgccagcatg gttaacagat ggtatggcta tcggtggggg
    56701 aatggtcgta gctgtaggtt acgctttggt aatcaacatg atggctacaa aagaagtatg
    56761 gccattcttc gttattggtt tcgttgtagc tgcaatttct caattaacat tgattgctct
    56821 aggtgcttta ggtgttgcat tagctcttat ttacttgaac ctttctaaaa tgggtggttc
    56881 atcaaataat ggaggcggaa gcaactctgg tgacccttta ggcgacattt tgaatgacta
    56941 ttaatcttga aggaggaaaa taaaatggca gaagaaaaaa tcaaattatc aaaaaaagat
    57001 cgtttagctg ttgcgtggcg ctctaccttc attcaaggtt catggaacta cgaacgtatg
    57061 caaaacggtg gctgggcatt ttctatgatt ccagcaatca aaaaattata caaaacaaaa
    57121 gaagaacgtt ctgcagcatt aaaacgtcac ttggaattct ttaatacaca cccatacatt
    57181 gcttctccaa ttttaggggt tacattggct ctggaagaag aacgtgcgaa tggcgcgcct
    57241 gttgatgatg tagcgattca aggggttaaa gtaggtatga tgggtccttt agctggtgtt
    57301 ggtgacccag tgttctggtt tacagtacgt ccaatgctag gtgcactagg tgcttcatta
    57361 gctatgggag gaaatatcct tggaccaatc atcttcttct taggttggaa cattattcgt
    57421 tgggcattca tgtggtatac acaagaattt ggctacaaag ctggttctaa aattactgat
    57481 gacctttctg gcggattgtt acaagacgta acaaaaggtg cttcaatttt agggatgttc
    57541 gtattagctg cccttgtaca aagatgggta tcaatcaaat tcttaccagt tgtatcaact
    57601 gtaaaattag ataaaggtgc ttacatcgaa tgggataaat tgccttcagg tggtgaagga
    57661 atcaagagtg catttgaaca agttaataat ggcttagccc tttctcctga aaaggtaaca
    57721 acattacaag acaacttaga ccaattgatt cctggtttag ctgcattagg attaacattg
    57781 ttatgtatgt ggttgcttaa gaaaaaggtt tctccaatcg ttatcatcct tggtttattc
    57841 gtagtcggtg ttgttggaca cgtcatcgga cttttataat ccataaaaat atagctcggg
    57901 cgttgtcccg agcttttttt atatagtata ctgttcataa acaaatatct ggagggagaa
    57961 aatggttcaa tcaatcaata caaaagtaga cttggtaatt gatgctactt cgtttacggg
    58021 tctgacagat tacgggcaaa tcatgatagg tgataatggt tttgagtttt accatgcgcg
    58081 agatagacgc aaatatatcc aaattccatg ggaagaagtg gattatgtac tagcatcagt
    58141 catgctaaag ggaaaatgga ttcctcgata tgccattcaa acgaaaaaaa atggtacata
    58201 cacattttct tccaaagaac ctaaaaaagt gcttcgtgcg atcagagaat atgtagatga
    58261 agatagaatg gtacaatcat tgagtttctt tgatgtagtg aaacgtggta ttcgtagtat
    58321 gtttaaacgc aagtaaaaaa taaagaaaac ttctaagagg aggttttctt tattttttta
    58381 ctttgcagaa gaaaatatga aatagcaaaa ccaattattt gtccccttta aatcccatat
    58441 gctaaaattg attagcaaag aaggaggtta tattatgagt tggttatgga cattgattgt
    58501 tggagccgtg atcggagcaa ttgcaggtgc gatcaccagt aaaggaaatt cgatgggctg
    58561 tgtaacgaat atcattgctg gactagtcgg ttcaagtatt ggacaagcga ttttcggcga
    58621 atggggacca aaagctgcag gtatggcact tattccatct attttaggcg cagtgatttt
    58681 ggtagcagtt gtctcatttt tctttggaaa acgatcataa gtttttacaa aatagtaaac
    58741 taaaaggagt tgcagcgaga ctgtgactcc tttttggctt tatttattta aaataaaaag
    58801 aagttttccg gaaattttta tttaactgtc gtgtacgaag aaaagtttgc tataatggaa
    58861 aaaagcgtgc aaagaaagag ggagtttcat tatgatgcta cgtgtagaaa aattgcgcaa
    58921 aaaaatgcaa gaagaaaatt tggattcatt tttggttacc agtccgtaca atcttcgtta
    58981 cttaacgaac tttacaggta cgacaggatt agctgttatt actttggaaa aagcattttt
    59041 tattactgat tttcgttata ccgagcaggc ggcagcgcag gctcaagggt ttgaaattat
    59101 taaaaatgtc ggacctattt ttgatgaggt agcaaactta gtacgcaaag aagaactgca
    59161 ctcattagct tttgaggaaa caacagtctc atttttagaa tattcggttt tagaagaaat
    59221 catcgatgcg gaattagtac ctgtttcaca gatgatcgaa gaactcagag aagtgaaaga
    59281 tgaagaagaa atcgctatta tcgaaaaagc atgtcatatt gcggatatgg catatgatca
    59341 catcctgaaa atgatccaac cgggcatgac tgagattgaa gtcgcaaatc aactagactt
    59401 ttatatgcgt tctcttggcg cttcaggtgt ttcttttgat acgatcgttg ctagcggatt
    59461 gcgttcggct atgccgcatg gtgtagcaag caaaaaaatc atcgaacaag gtgacttgat
    59521 cacattggac tttggttgct attacgaagg gtacgtttcc gatatgacac gtacattcgc
    59581 tattggtgat cctggggaaa aattaaaaga aatttatcaa atagtcttag atgctcaatt
    59641 agcagttatt gatgctgcaa aaccaggaat gactggtgtc caattagatg cagttgctag
    59701 agattatatt tcaaaacatg gttacgggga agcatttgga cattctacag gacatgggat
    59761 cggtttagag atccacgaag gaccaaacgt ttcttctcgt gcagaaaagc aatttgtacc
    59821 aggaaatgtg atcactgatg aaccagggat ctacttacca ggaatcggtg gagtacgtat
    59881 tgaggatgac ttgttgatca cagaagatgg aaatcgtgtg ttgactcgtt caccaaaaga
    59941 attgatcatc ttatagtttc atcaatagaa gaagtaagcg taaaaatcag tgttcacaga
    60001 ctttgttata gaaaatcact cgatttaatg atacactata gaagaacttt taagctgtaa
    60061 gagataataa agttgggtgt tttctccaat tcatagcagt ttgcagctga tcggcggcag
    60121 ttttcttacc acgtgatcac tatgccgcat catagagaat tcggatgtat ctcgtgctta
    60181 ggcaaaggga ggaataaata aatggcagat gaaaaaaatt tgattttaaa cgcaaaccaa
    60241 gatttaggag agatcgtgat cgctcctgaa gtcatcgaag taattatcgg gattgcagct
    60301 tctaaagtag aaggcgtata cggaatgcgc ggaacttttg ccagcaatgt cactgaatta
    60361 ttaggacgtg cagctcacgg aaaaggtgtt tacttagcaa acgaggaaga tggcttgaaa
    60421 gtagatcttt actgttatct agaatatggt gtttcagttc ctaaagtagc aatggaaatg
    60481 caggaacggg tcagccagca agtccttttc atgacggata tcgatttatc tgaagtcaat
    60541 atccatgtag tagctgttgt tcctgaaaaa ttagaacaac caacaatcga agaacttttt
    60601 gaagacgaag aggaagagaa tgaataaaga attatcaaga cacgagattc gggaaatggc
    60661 attgcaggct ttatttccat tagacttcaa tgcagaccta acaaaagaag atgcgatttt
    60721 caacgcaatc gaattagacc accgcgacat gatcaacgaa gatgaatcag agtttgttcc
    60781 tgtttatctt gatacgctag ttggcggtgt ctgtgcaaaa aaagatgaat tggataaagt
    60841 gattgaaaaa catttaaaaa acaattggca catcaatcgg atctcaaaaa tggatttggt
    60901 gatcttgcgt atcgcgattt ttgaaatgac ttatgtgaca gatgtaccag cggctgttgc
    60961 tttaaatgaa gcaatcgagt tggcaaaaac atttagtgat gaccgttcac gtaaattcgt
    61021 caacggtgtt ttatccaatg tattgaaaga attagaagct ggggcgtaag tcctagcttt
    61081 tttctatgaa tcatcataaa cctgttataa atcgggaaaa ttctatggta gaatgaaaac
    61141 acaaaaaata tctgggagtg gttagatggc agaattgatt aacggaaaag aattagctga
    61201 aaaaatgcag gcagagattg ctgaaaaaat ccaaaaattg aaagataatg gtatccatcc
    61261 cggattagtc gttttattgg taggggaaaa tccagccagc caaatctatg taagaaataa
    61321 agaacgtgta gcaaaagaaa tcggaatcca ttcattggta gagcgttatc ctgagacgat
    61381 ttctgaagac gaactacttg cagaaattga aaaatacaat caagatccta gttttcatgg
    61441 tatattggtc caattaccat tgccaaagca tatagatgaa gaaaagatcc tgttagctat
    61501 ccgccctgaa aaagacgtag acggttttca tccgatgaat ttaggcagac tccttgcggg
    61561 taaaccggac aagattcctt gtactccata cggcatcatg aaaatgtttg aagcttattc
    61621 tatcgatccg gcgggtaaac gagcagtagt tatcggacga agcaatatcg tcggtaaacc
    61681 gatggctcag ctactcttga tggcagatgc aactgttact gttgctcatt ctaaaaccga
    61741 aaatctggct gaactggcac aagaagcaga tattttagtc gtagcaatag gacgtggaca
    61801 ttttgtcaca aaagaattcg taaaaccagg tgctgtagtg atagatgtag ggatgaatcg
    61861 tgatgctaac ggtaagctga tcggtgatgt gaaatttgat gaagtggagc ctattgcagg
    61921 ctggatcact cctgtgccaa agggtgtagg gccaatgact atcacgatgc tgatgtatca
    61981 aaccgtaaaa tctgccgaag cttttggagc aggtgaatag atggcacaag aatatttaac
    62041 tgtcactaca ttaacgaagt atttgaaaag aaaatttgat gccgatcctt atttagaacg
    62101 cgtatattta acaggcgaaa tatcgaattt ccgtttacgt gccaatgccc atcaatattt
    62161 cagcttaaaa gatgaccatg caaaaatatc tgctattatg ttcaaaggag cattccaaaa
    62221 attacgattc cagccaaaag aaggaatgaa agtccttgtc gttgggcgta tttcattata
    62281 tgaagcggga ggttcttatc aaatctatgt agagcacatg gagcctgatg gaataggtgc
    62341 tttatatcaa gaattggaag aacgaaaaga aaagctgatg aaggaaggcc tttttcaagg
    62401 gcctaaaaaa atgctgccac gtttcccaaa gcgaatcgcg gtccttacca gtccaagcgg
    62461 agcggttatc cgcgatatca tcacgacggt gaaaagaaga tatccaattg tccaattggt
    62521 tttgttccca acagttgtac aaggaaacca agcagcggac aatgttgtca ggaatatcca
    62581 gagagtagaa gagatgggca atttcgatac aatgatcatc ggtcgtgggg gtggttcgat
    62641 tgaggattta tggccgttta atgaagagcg ggttgctcgg gcgatttatc aagctaatac
    62701 gccggtgatt tcatctgtag gacatgaaac agataccacg atcgctgatt tagtagcaga
    62761 tgttcgggct gccactccaa cagcagctgc agaattagct gttcctgttt tgtcagaaga
    62821 gttgatgcgc attcaagaac ggcaagctag attgcagcag gcgtttgtac gccagatcca
    62881 gcgcaaacaa gaacggttcg agcgtgctcg acgttcctat gtatttcggc agcctgaaag
    62941 attgtatgaa ggtcagacca tcaaattaga ccagttgaaa cagcgattat atcaagctac
    63001 acagcaaatt tatcatgcga aagaaaaaca aacgatcgca ttaagccacc aattggaaca
    63061 agtggctccg ctttatcgag ttaaagcagc tcgccaagaa actgcttatc tagaaaaacg
    63121 cttagaagag aaaatccaac aatatatgaa acaacagcag cagcgttttg ctcaagctgt
    63181 tcaatctctt gatttgttga gtccattgaa aatcatggga agaggctaca gctatacgac
    63241 gttaaatgat caagtagtaa aaagtgtcac cgagattcat caaggggatt tgctgacgat
    63301 ccattatcaa gatggaatcg ttaaaggaca agtacaagag atagacaaag gagaataaaa
    63361 atatggaaaa tccaactttt gaagagtcac tgcaagaatt agaaaaaatt gtcatgcagt
    63421 tagaacaagg agacgttcca ttagaatcag ctctagattc gtttaaacga ggaatggaat
    63481 taagcaaaca ttgccaagat acattagcta aagcagaaga gaccttgacg aaaatgatga
    63541 cagaatcaaa tgaagaagtt gattttgacg gaaacgagga aagctgagat gagattaact
    63601 gacttttcag ctattcatct acctgctgta gaaaaagaaa tcttgtcttt tttagatgag
    63661 catacaacgg atagaggact ctatgatgcg atgagttatt cagtgaaagc aggcgggaaa
    63721 agaatacgtc ccttattact tttaacggcc gttgcttcct ttgatgagcc tattgatgtt
    63781 cccgtctatc aagtagcagc tgctttagaa atggtgcata cgtattcctt gatccacgat
    63841 gatttgcctg cgatggacaa tgatgatctt cgtagaggaa aacctaccaa tcataaagta
    63901 ttcggcgaag cattagctat attggccgga gatggtttgc ttacaggagc attccagcta
    63961 atcagtatgg cacacttagg taattctcct aagcttctgc tgcttcagca attagctgtt
    64021 tgtgcgggat cacaaggtat ggttgctggg caagctgcag atatcgaggg cgaatcgaaa
    64081 aagctttctt tagaagaatt agcatttatt catgaaagaa agacagggca tttgattcgt
    64141 tatgccttgc tggctggtgg catattagcg aagcaacctg aagaaatctt attgctactt
    64201 caacgattag ccgaacattt aggtttagct tttcagataa gagatgatct actcgatgtg
    64261 attggtacaa cgaaaacatt agggaaaaca gctggtaaag atgaacgtat ggaaaaaaat
    64321 acttacccgc gtttattagg cctagaaaag acacgtgagg cgttggagat tgagctgtta
    64381 tccgccaaca agatcataga caaattagaa gaggacgttt tttcttttga tggcgggctg
    64441 atgcgccaga tgatcaagca atttgatgtg gagaaatgag ggaaagaatg gaaaaagaac
    64501 gcgtagatgt attagcggtc aaacaaggat tatttgaaac aagagaacaa gccaaacgtt
    64561 cagtgatggc tggtttgatt tacaacgaaa aaaacgagcg gtttgataaa ccgggagaaa
    64621 aaattccagt gagtagtgaa ttaagagtca aaggtaaaaa acttccttat gtttctcgag
    64681 ggggattaaa attagaaaaa gcactcaaag catttgatct atctgttgaa ggaaaaacat
    64741 tgttagatat tggtgcatcc actggcggat ttactgatgc agctttgcaa aatggcgcaa
    64801 aaatgagtta tgctttagac gttggctaca atcagctagc ttggaaaata agacaagatc
    64861 ctagagtcgt cgtaatggaa agagtgaatt ttcgttatgc aaaacccgaa gactttacct
    64921 taggaattcc agaagttgct gttatcgatg tttcttttat ttcactaaaa ctgatgctgc
    64981 ctccattaca tgctatttta aaagaagagg gagaagtgat tgctttaatc aaaccccaat
    65041 tcgaagcagg cagagaagct gtagggaaaa acggaattgt tcgtgaccct cagacccatc
    65101 aaaaagtttt agaagacatt ttgtcatttg cggcacaagg tggatatgat gtgctggaat
    65161 taagttattc tccgatcacg gggggagaag gaaatatcga gttcttggct cacttgagga
    65221 aagtacccga aagcggaaca atcaattcag ctattaatat ggcagaagtg gtttcaaatg
    65281 cacacgaaca gtttgaccat aaataaattg taactgtgaa aatcaagtga gaagtatgcg
    65341 gattcttctt tattatacca aggaaataag ctatcattga ttaagaagaa tatgctaagg
    65401 agcgaagacg taatgagaaa aaaagatcgg catcgtttga ttactcgatt gttgagtgag
    65461 caagatatcc gaaagcagga agaatttgtt gaaatcctga aaagcaaagg aatatctgtt
    65521 actcaagcta cgatttccag agatataaag gaactgaaac tgatcaaggt accggcttca
    65581 gatggcggct accgttatag cataccagct gaaacaaacg aagatgtaaa tactaaattg
    65641 gaaaaactat taaaagacgc tttcgtggca gttgaccaaa tggaaaaatt tgttattttg
    65701 aaaacattgc cgggaaatgc ttcggcagca gcaaatttga ttgataaacg ttacaagaaa
    65761 gaattatttt cgattatcaa tgatgacgat aatgttttga tgattacacg aactgaagac
    65821 caggcagttt tattgaagaa agacttcctg cattaccttt aagttgagga gcgaatgtga
    65881 tgctacaaga aatcagtatt actaactttg cgattattcc ggaattgcgg ctttcttttc
    65941 acgaaggaat gaccgcgctg accggagaga ctggtgcggg taaatccatt attatcgatg
    66001 ctttaggtct gttagcagga ggaagaggtt caagcgatta tatacgtcaa ggagcagaaa
    66061 aatgtgtgct tgaagggttg tttgaactac caaaacaaga aggattctct gaactgatgg
    66121 ttgaattagg gatcgaaaca gatgaggaca atctgattgt tcgacgggat atgtccttaa
    66181 ctggaaaaaa tgtttgtcga gtgaatggtc atattatcac attagctaat ctgagaagaa
    66241 tcggcagtta tttagtagat atccaaggac aaaacgaaca tcaggaactg ctccagcctg
    66301 aatcacatct tgctttattg gatcgttttg gagatgtagc tttccaacag aagaaaaaga
    66361 gttatcagca agaatatatc tcttatcgtg agctggaaaa aagggtacga aaagtgcagc
    66421 aaaatgaaaa atcctatgtt cagagaatag atatgctcca tttccagcaa gaagaaatag
    66481 ctgctgctga tttgcaagtc ggagaagaag agaaattaaa agaagaaaga gaaaaactta
    66541 gcaattatca aaaaattgct gatggattag ctgcaggtta cggagcactg acgaataatg
    66601 aacaaaacag tgtagatggt gttggcttgg ccgtttcaga actgcaaggg atcgcccatc
    66661 tagatgtgga gtatgaagca atttatgaaa atatccaaag tgcttactat ttgctgcaag
    66721 atgcaatcgg cgatatgagt cgtcagattg atttattgga gttagatgag agtcgtttag
    66781 aagaagttac gcaacgttta gaattaatcc gacagttgaa acgtaaatac ggcgaatcca
    66841 ttgagtcaat attggcttac tatgacgaaa tcaccgaaga gctagcttct tctgattttt
    66901 cagaaggtca attggacaaa atgaaagagg aactagagca aaaagagctt ctacttcagc
    66961 aacaagccgc agatcttcat gaagcgcgta aaaaaatcgc aaaagaattg gaaaaatcca
    67021 ttctgcatga attgaaaagt ctgtatatgg aaaacactga gtttgaggta cgtttcttaa
    67081 aagaagaaaa tagacaattg aatcatgatg gatttgacca aatcgaattt tacattacga
    67141 ccaatccagg agagccactg aagcctttgg tgaaagtcgc ttctggtggc gaactgtctc
    67201 ggatgttgct tgctttgaaa actatttttt cttctgagca aggtgtgaca agtattattt
    67261 ttgatgaagt ggatacaggt gtgagcggaa gagttgcaca ggccatcgca gataaaattt
    67321 ctcaaatatc gaaatactct caagttttgt gcatcactca tctgcctcaa gttgctgcag
    67381 tcgccgacta tcaatattat attgtgaaag cagtgattga tggacgtacg caaacttctg
    67441 tttcagagtt gaaaacaaaa gaaagagaag aagaaattgc acgtatgctg gctggaagtg
    67501 agattacaaa actcacactc gaacatgcaa aagaactatt gaaactcgca aaaaggacct
    67561 gagccacctc gcagtggttc aggtcctttt cagcagctga tagctatatt ttatttttac
    67621 atgacactca ttacagcagt caatacaaca gatccaatcg ttaaaatcag cattgtccaa
    67681 atgacaaatt ttgttacttt agcaaaagta cttgtttgtt ttttttcacg cattgttttc
    67741 aacctcgtca ttaattagtt gattcaagtg tacaatcttt tttccatttc cacaagaggg
    67801 cttttttata aaaacttccc tttttttaaa taataggtag ttacgataca aataaataat
    67861 gttaatagaa ctagccatat aaaggcatca tccctttcag caataggtaa cttaatgttc
    67921 attccataga tactgccgac gattgtcggt atagttaaaa taatcgttaa agaagttaaa
    67981 attttcataa tattattcag attattcgat acgattgctg aaaaagtctg attcatttgt
    68041 tctactagtt ttaattgaat gcgagaagta gtcattgcct gctctgtttc caccaataca
    68101 tcacgtaaat gatttttcaa agcatgattt tcttggaaaa gtttattttg cgctagttct
    68161 ttcaatgcat ctaaattagc agaagtcgct tctctgaaat aaacgaggct tttttggata
    68221 tccatgattt gataaagttg tttgttttca gtggatactt gaagtttggc ttccaaagta
    68281 tcgcattgag attgaagcgt agctaaataa ctgttgtaag agagtgtgac ctgccaaatg
    68341 aattgaaaga agatattgag attaggcgaa atatcacttt ttggaaaggt ctggctaaag
    68401 actttcttta aaaacagtgg ttcatgattc gtaattgtga taagcttctt tttgggcgtc
    68461 aaaataatag agagcgggta tgtatccact tgaaaataac cgctaggact tgttttagca
    68521 aaaggatatt gtaacaataa taacgctggc tttgtcaact ttgattgatt gaattcttct
    68581 gtacgggaat tttctgcatc gtccaatatg gaagtgatgt aatctgacgg aatttcaaat
    68641 tgatgtgtca gctgatgaat ctcttcttga ttaggacgtt ctacacaata ccagatcgtt
    68701 tggtcagttt cctttccggt attacgcagt ttctcattat ctaatgtaaa atatttgatc
    68761 atgattttcc tcctgttgcc tgtttctttt attataacgg atagggaatt ggaatacatg
    68821 gaatgtgttt gtaagatata gaatataact gcataaattt aaataaaatg aaacagttgt
    68881 gtaaagtata tacagtatac ttgaagcaac gataaaaaag gagtttacgt tcatgggcac
    68941 attcacacca cttatcttgt tttggtatat ttacccggta atcgttttgt ttggttgcca
    69001 atttcttgta aaattatttt cgttaaatcg tcgttttaaa ataaaagccc ctgatttggc
    69061 gattcctttt ttgtggggcg gtatgcatgc attgtctaga aatacaggga caatttctat
    69121 cttaccgttt ttctttattt ctgttttgct aatgggaatt tgtattgctg ttttccaagc
    69181 atattattat gaagaaatta tttatccgcg ttatttcaag atgacttggc gtttggtttt
    69241 tctgttgaca atcgtcttgt acgctgtctt gatcattgtg aacatacttt cttacctatg
    69301 aaaaaaatag aaaagctgta aaaagtcttt taaaaggctt tttgcagctt gatttttaaa
    69361 aaaatgttta tgaataaaaa aagttgttcc cggatttccc agaaaaggtg gtagaaagtg
    69421 ggggattgtg gtagactaat tttagttagt ggaggaatgg ggggctttca atgttgatgg
    69481 gtgaattcca acataatatt gatgcaaaag gacgtttaat cgtgccctca aaacttcgtg
    69541 aagaacttgg cgaaaagttt gttctgacac gcggattgga tggctgtctt ttcggctatc
    69601 cgatgagcga atgggaaaat ctggaagcta aactgaacga aatgccgttg gctaaaaaag
    69661 acgcacgtac ttttgtgcga ttcttctatt cggctgccac tgaatgcgaa cttgataaac
    69721 aaggacgaat caatataccc agcacgttaa gaaattatgc agctttgaca aaaggttgcg
    69781 tagtcatcgg tgtatctaac cgaattgaaa tttgggatga gacacgatgg caagattttt
    69841 ctgcagcagc agaagagaac tttgacgaaa ttgctgaaag tatgattgat ttcggactat
    69901 aagaatagga agaggctgac taatgactga aacatttcaa cactatacag tcatgttgaa
    69961 agaaacggta gatggattga acatcaaacc ggatggcgtt tatgtagact gtacactcgg
    70021 cggtgccgga catagtgaat atttattaag ccaactaagt tctaaagggc atctgtatgc
    70081 gtttgaccaa gatcaaaaag caatcgatca cgccaagctg cgtttagctt cttatgtcga
    70141 acaaggtatg gtcacattta tcaaagcgaa cttcagagaa ttagaagaac gcttgagtga
    70201 gcatgtagat aaggtagacg gcattttgta tgatctaggg gtttcctctc ctcagctaga
    70261 tgaagcagaa cgtggattta gttatcatca agatgcaccg ttggatatgc gcatggatca
    70321 gtcagctcct ttatccgctt atcacgtaat taatgaatat tcctaccatg aattagtaaa
    70381 gattttcttc cgctatggcg aggagaaatt ttccaaacaa attgctcgtg aaatcgaacg
    70441 agtacgaaaa acaaaaccaa tccaaacaac tggtgagctt gtcgaaatca ttaaacaagt
    70501 catcccagct cccgcaagaa gaaaaggcgg gcatcctgca aaaagaattt ttcaagctgt
    70561 gcggattgca gtaaatgacg aactcggtgc cgaagaggcc tctttagaac aggcaatccg
    70621 gttattgaaa gtcaatggaa gaatcagcgt aatcacattc cattcattgg aagacagaat
    70681 tgtcaaaagt atgtttaaag aatatagtac ggtacaagat ctaccaccgg gacttcctgt
    70741 catacctgac gaattccaac cagaactgaa agtcattact cgtaaaccaa tccttccttc
    70801 agaggaagag ctaacagaaa acaatcgatc acgcagtgca aagttacgta ttgcagaaaa
    70861 aataagaatg aaagaggagt agaatgatgg cagaattaaa gaaaatgcaa gattacgaat
    70921 atgatttacc tgaattggtg gatcagccgg aacaacaaat gcctgattct tcttcgaatc
    70981 aagaaatcat tcttccacaa tcaccggcaa aacgattaaa gcagatttct gctttggaaa
    71041 aaattgctgt ggcaagcgtg gcgattgcta ttgttgcttt atgtgtttta acagttatgc
    71101 tgcgcacaaa tatcagcgga gctgaaaaag aaatcacgtc aatacaaaca gaaatcaatg
    71161 ataaaaagca agaaaaaacg agtttagaac aagaaaaaaa cgaactttct cgtactgaac
    71221 ggattaaaca aatcgcagaa gaaaaaggat tgtctattaa tgacgacaat ttaaggaaag
    71281 tgaagtaaat gagtcttaaa aataaaattc ggcggtttat ggaaaagaaa aatctaaatc
    71341 cgatgaacaa tcgcaaaaaa gtaggtatca ttttatttgc tacgagtatt ggattgttct
    71401 ttttatttgc cgtccgtttc tcgtatatcg ttattggagg tcatgttgct ggtacctcat
    71461 tagctgaaaa aacaaaacag ttgtaccaag gaagtgaagt agtaaaagca aaaagaggaa
    71521 cgatttatga tcgtaacggt gtggcgctcg cagaagatgc ctcctcttat tcaattaaag
    71581 cgatactctc aaaaacatat acctctgggg ataaaaaact gtatgttgaa gaaaagaatt
    71641 ttgataaaat agcagaaatc ctgcataaga atctttctat agacaaaaaa gatgcactga
    71701 atattttaga ggacggcgca aaaaaagagc tgtatcaagt tgaatttggt agctatggga
    71761 aaaatatctc ccaagaaacc aagcaaaaca tcgaagcgga catgaaaaaa gaaggtgtcg
    71821 ctggtcttta ttttgtcgac catcaggctc ggatgtatcc aaatggcgtc ttttcttctc
    71881 attttattgg atacgcagtt ccagacaaag atgagaatgg cttagttggt aaactaggtt
    71941 tggagtctgc ttacaatgat atcttgagcg gtaaagatgg taagattatt tatcaaaaag
    72001 ataattttca aaatcctctg ccaggtacag tggcagaaga agaaaaagct gtcgacggac
    72061 aagatatcta cacgacttta gacagcagac tacaaagtta tttagaaaca ctgatggatc
    72121 aagtaaatga agaatatcag ccagaagaat tgacagcagt cctgatgaaa gcaaaaaccg
    72181 gagaaatctt ggcaatggga caacgaccta ctttcaatcc tgaaacaatg gaaggattga
    72241 ctggaaaaga tgctatctgg cgtaatttct tggtacaaga tagttatgaa ccaggatcaa
    72301 ccatgaaagt tttcactact gcagctgcta tcgaagaagg agaattcaac gagaatgaaa
    72361 ccttccaatc tggaaaaatc caagtagaag atgctacgat caatgaccat gactttggtg
    72421 aaaaaggtgt gctgaccatg cgtcaagctc tttcatggtc aagtaacgtc gggatggtca
    72481 tactggaaca gcgattaggc ggacgatggt ataactattt acaaaaactg ggatttggac
    72541 aaagtactca ttctggatta gatgatgaag taaacggtgc tttacctaca ttaaatattg
    72601 tcgaccgagc aatgagtgct tatggacaag cggtaggtgt gacgaatttc caaatgatga
    72661 aagctttcac ttctattgcg aataacggta caatgattca acctcgatat atcagcaaag
    72721 tggtggatcc ccaaactgga gaagagcgaa caactcaaac agaagttcta gggcaacctt
    72781 tttcaaaaga aacaacagaa aaagtccgtg aatatatgag agatgttgtc gaaagcgaaa
    72841 actacggtag tgcttatggt gtatatagtg tacctggata caatgtatcg gcaaaaaacg
    72901 gaacagccca aatcgcttct gatacagggg gatatcaaac aggagataca gcctacctgt
    72961 attcgattgt ggagatggtt ccttctgaag atccagacta tgtcttgtat ctaacaatga
    73021 aacaccctaa gacatatgat cgaatggcat tggctaaaat cgcaaatcca ttgatgaaac
    73081 gggcaatgga ttttaaagaa actgaagaag attcagatac agaaacaaaa acagaaaaag
    73141 tatccgtagc cgattatcgt aatcttgaag cggacgttgc agcagctgat gctcaaaaaa
    73201 gtggactgca acctgtagtg atcggtaatg gtaaaaaagt acaaaaacaa tctactgcca
    73261 acggtgatca gttgatctct ggagaaaaac tgatcctata tactggtggt gataagctga
    73321 tgcctgacgt aacaggatgg tcaaaagcag atatcatgaa gctggggaaa atcctcggta
    73381 ttgaagttag ttttgatgga gatggttatt gtgtcaaaca agaacttgct ccatatgaaa
    73441 aaatcactga agacaaactt agttttactt tagaagaata agaagagaaa taggagagaa
    73501 aagagcatgg aatggacaca agcattgatt cccattgtca gcagttgtgc aatgacaatt
    73561 gccgcaatgc cgctatttat tggttatttt caaatgaaaa agcaaggtca ggcgattcgt
    73621 gaggagggac caaagtggca caatagcaaa gccggaactc ctacaatggg cggcttagtg
    73681 tttctgatcg gctctatttt gacaggaatt tgggttggag cttggcaaaa acagctaact
    73741 cctactcttt tcatcttgct ttttgtatta gcgttatacg gagtaatcgg ctttcttgat
    73801 gattttataa aaatattcaa gaaaagaaac atgggactga actcgaagca gaaattgatt
    73861 ggacagattc tagggggaat catcttttat cttgtttacc gttctgaagg ttatccaggt
    73921 gtattgaatt tcttcggact agatctacct ttaggaatca tttatggtat tttcgctctc
    73981 ttctggcttg ttggattttc aaatgcagta aatctgacag atggcatcga tggattagtt
    74041 gctggcttag gaagtatctc ttttgcagct tacggtctaa ttgcttggca tcagcatcag
    74101 tatgatgtat taatcatttg cttaagtgtt ttaggaggtt tactgggttt cttcgtctac
    74161 aacagaaaac ctgccaagat ttttatggga gatgtcggtt cattagcgtt gggcggactt
    74221 ttagcggcaa tttctataat gctaaaccaa gaatggacat tgcttttgat tggtttgatc
    74281 tatgttatgg aaacagcaag tgtgatgctt caagttactt cctttaagct tacaggaaaa
    74341 cgaattttta aaatgtctcc aattcatcat cactttgaaa tgtgcggatg gtcggagtgg
    74401 aagattgata cagttttttg gttgatcagc attgtgactt cgttgatcac attatggatc
    74461 gtttggtagg agatgacggg aaatgaaaaa aatatcaaca tatgaaaata agaaagtgct
    74521 tgtcttagga ctcgcaaaaa gcggagtaag tgcggccaaa ttattacatg aattgggcgc
    74581 acttgtcacc gttaatgatg gaaaaccttt tgacgaaaat ccggaagcac aagagctgct
    74641 ttctttaggg ataaaagtaa taacaggtag tcatccaatc gaattgttgg atgaagaatt
    74701 ttcattgatg gtcaaaaatc cagggattcc ctacagtcat cctttagtag caaaagccca
    74761 agaaatgggt attccggtaa ttacggaagt tgagctagct tacgaagtag cggaatgccc
    74821 aattattggg attacaggaa cgaacggaaa aacaacgaca acaacgatga caggactact
    74881 actgaatgca ggagcagatc aaggaattgc acgtttagcg ggtaatattg ggtatccagc
    74941 aagtggtgta gcgcaagaag caaaatcaga agataaaatt gtgatggaat tgtctagctt
    75001 ccaactcatg ggaatcacag atttccgccc tcatatagcg gtgattacca atatctatga
    75061 agcacatatt gattatcatg gtacaagaaa agaatatgtc aaagctaaat ggaaccttca
    75121 aaaaaacatg acagaaaaag attatctgat ccttaactgg aatcagtcag aactacaaga
    75181 attagcgcaa agaacaaaag cacgcgtact gcctttctcg acaaaagaag tgttggaaga
    75241 cggtgtgtat gctgacgatt actcaatata ctataaaaaa gaaaaaataa tggaaatatc
    75301 tgaacttggc gtaccaggta agcataatgt agaaaatgca ctagctgcta tttctgtagc
    75361 aaaattgtat ggtatatcaa atgaagctat acgagaaacg ttgcattttt tccatggcgt
    75421 accacatcgt acgcaatatg taggagaaat ccaaggcaga aaattttata atgattcaaa
    75481 agcaaccaat attttagcga ctaaaatggc tctaagtgga tttgaaacgt ctaaggtcgt
    75541 gttattggct ggcggattag acagaggaaa tacatttgat gaattaattc cttcattaaa
    75601 aggaatcaaa gcaatggtcg tatttggcca aacaaaagaa aagttgatgg atgctggaaa
    75661 aaaagcaggc atcgagacaa tagtaacagc cgattctgta gaacaagcag ttcctttagc
    75721 attagaaaac agtacagatg gtgatgtagt tttattatca ccagcgaatg ccagctggga
    75781 tcaataccca aattttgaaa ctagaggaaa cagattcatg gaagctgtga accgattaaa
    75841 atagaaaaag tagaagcgag cgataaaaaa tgaaaatttt ggttacaggc ggaggaacag
    75901 ggggacatat ttatccagct cttgcctttg tcaattatgt aaagactcaa gaaccgaatg
    75961 ctgaattcat gtatgtggga gcaaaacgag gtctagaaaa caaaatcgtc cctgatacag
    76021 gaatgccttt ccataccttg gagattcaag gatttaaacg aaaaatttcc atgcataaca
    76081 tcaagacggt ccgattgttt cttaagagta tccgtcaagc aaaaaaaatc ctcagtgatt
    76141 ttcaaccaga tatcgttatc ggtactggag gatatgtttc cggagcggta gtgtacgctg
    76201 cagccaaaat gggtattcca acagttatcc atgaacaaaa cagtgtacca ggtgttacca
    76261 ataaattttt aagccgatat gtggatcgga tcgctctttc atttaaagat gcggcttcct
    76321 tttttcctga aaacaaagca gttcttgtag gtaatcctcg agcacaagaa gtagcagata
    76381 ctaaaaaatc agatattttg aaaacatttg atttggatcc agaaaagaaa acagtcttga
    76441 tttttggcgg tagtcaagga gcacttaaaa tcaatcaagc agtatcagtt tttttaagtg
    76501 aatatattcc ttcttcttac caagttttgt atgcttcagg agatagatat tatgaagaga
    76561 tcctaaataa aataaatcct gaaatacaaa atattagtgt ccagccttat atcaaaaata
    76621 tggcagaggt catggcttcc agtgatctgc tggtggggcg tgctggtgca acttcgatcg
    76681 cagaattcac agctctgggg ttaccggcag tgttggttcc cagcccatat gttacgaatg
    76741 atcatcagac taaaaatgca atgagtcttg ttcacgcagg agcagcaaaa atgattgcag
    76801 ataatgaact aacaggtgaa tcactaagtc agacagtcaa cgaaatcatg ggtgatgaag
    76861 aattgcaaaa gcaaatgtgc cgagcttcta aagaacaagg aatccctgat gcttcaaaac
    76921 gtctttatga cttagtaaaa caaataatca aaaaataaag gcggtggtct tagtgagtaa
    76981 gaaaaaagaa ccaaaaaaat tgtctcatcc gtcttctgat gaaaacaatt tgactccttg
    77041 gcaaaaggct aaccgaaaat atctggcaga acaagagatg aaaaaccaag agccaaaaaa
    77101 agaagacata acattagata cacccctttc agattctgaa gaagaaaaaa cggttcaaga
    77161 ggaacagcaa gaagaaagcc agtccgagaa aaaaatcgaa ttttttgaag agatcaagca
    77221 aaagaaaaac ggtggaccat ataacggttc ttttttgaac cggctgccca atttaaaaag
    77281 tcaacggcac aaagtactat ttcgtcggtt agctttgatc atcactgtat tgggtatacc
    77341 gctgattttt ttgatctatt atgtttctcc ttatagcaaa ttacaagcag ttgcagtatc
    77401 aggcaataag acagtcaaca gccaagaaat catctctgat acaaaattgt cattaggaga
    77461 aaatgtttgg ggacagtatt ttcatcgatc tacttatatc gatcgcctta aaaaagcaca
    77521 gccaagaatc gaaacagcaa atatacattt caaagggatg aatgaattcg atttggatgt
    77581 aacagaatat aaagagattg ctttgatcgc aaaaaacaat caatactacc cagtgattga
    77641 aaatggaaca gtattagatg aaaaagtagc aaatcctaca aaaaatctcc ctatcttgga
    77701 agagttcaaa gactctgcaa aaatcaaaga attaacaaag caatacaatc agttatcatc
    77761 agaattacaa aaagcaattt ctgaaatcaa gtacacgcca agagcaagca ataaaaatct
    77821 gatccaattg aatatgaatg acggcaatca agttattgta aatatcaata atcttgcgaa
    77881 ccaaatgaaa tattattcgc aagtagccaa agatatggat gaaaaaggtg tgatcgacat
    77941 ggaagttggt atcttctcgc atccttacgg tagcactcaa gaaacggaag aatcgggcca
    78001 atcggaagaa acaactgaaa gtgaagaaaa tgcggatcaa acaaacgaat caagtgaaga
    78061 ggcagaaacg caaaaagcac aaacacctgg atcgtctagc acaaacggat catctgattc
    78121 aaatgattca agtgaaacaa gtcaaataga agataatcca agtacaactc cgtcaagttt
    78181 aaatgaaaat tgataaaaca actttaaaaa gtaagaatgt actttctaaa aaccagtaat
    78241 ttatggtaga attggaagca gtgaatatat ctattaataa gtatatttta aaacggacgc
    78301 tagttgttcg cttgtatatg gaatagtaag taggagggaa tcccattcat ggcaaaaaca
    78361 ggaatgtatg taggccttga tattggaacg acatctgtta aagttgtcgt ggctgaatat
    78421 atcgacagcc aaatgaatat tattggagta ggaaacgcaa aatcagaagg gattaaccga
    78481 ggcattatcg ttgatatcga taaaacggtc caagcgatac aacgagcagt acgacaagca
    78541 gaagaaaaag caggtatcca gatcaaaggc gtaagtgtcg gtttgccagc aaacatgctt
    78601 gaagtagaaa attgccaggg aatgattgca gttaacggag attcaaagga aatcaccgat
    78661 gaagatgtca gaaatgtggc ttcagctgcc cttgttcgtt caatacctcc tgaacgtcaa
    78721 atcgtgtcaa tattgccaca agattttact gtcgatgggt tcgagggaat caaagatcct
    78781 cgtggaatga tcggcgtaag attagaaatg tacggtctgt tgtttacagg acctaaaacg
    78841 atcatccata acatacgtaa atgcgtggaa aatgccggat taatcgtcaa cgaaatggtg
    78901 attactcctt tagcgctgac tgaatcaatt ctttctgacg gagaaaaaga ttttggtacg
    78961 atcgtcattg atatgggtgg cggccaaaca acaacggctg ttatgcatga taagcaattg
    79021 aagtttacca atcttgacca agaaggtggc gaatttgtca ctaaagatat ctctattgta
    79081 ttaaatacgt catttaacaa tgcagaagcg ttgaaaataa actacgggga tgcataccca
    79141 gaacggacat caccagatga agaatttcca gtagatgtga ttggtcagtc agaaccagtg
    79201 aaagtcgatg aacgctactt gtcagaaatt atttcagctc gaatggaaca gattttcaac
    79261 aaagcaaaag atgcattgga ccaaatcgaa gcattagagt tgccaggtgg cgtcgtattg
    79321 acaggcggag ctgctagtct tcctggtgtt gtggacttgg cacaagaaat cttcggcgta
    79381 aatgtaaaat tgtatgttcc aaatcacatg gggctgagaa acccggtatt tacaaatgta
    79441 ataagtatcg tggattattc agcaaacttg agtgaagtgt atcaattggc aaaaattgct
    79501 gttacgggtg aaacctcagc agctcgtcaa atggtagttg aacaagaaac aacaaacaca
    79561 tatgagagct atgaagcacc tgaagaaaca atctacgatg agccggaacc aaaagagtcc
    79621 ggagaaaatg taaaaaataa aatcaagggt ttctttacaa atatttttga ttaattcatc
    79681 ctgggaggaa taagatatgg aattctcaat tgataataat atcaatgacg gcgcagtaat
    79741 caaagtcatc ggagtcggtg gtggcggtgg aaatgctgta aaccgaatga ttgaagaaaa
    79801 cgttaaaggt gttgaattca ttacagccaa cacagacgtg caagcattaa aaaattcaaa
    79861 agctgaaaca gttatccaat taggccccaa atatactcgt ggtttaggtg ctggttctca
    79921 gccagaagtt ggacaaaaag cggcagaaga aagtgaacaa tccttacgcg aagctttaga
    79981 cggtgcagat atgattttca tcactgcagg aatgggtgga ggaaccggta caggtgctgc
    80041 accaatcgtt gcaggtatcg cgaaagaatt gggcgcattg actgttggtg tcgtgacacg
    80101 tccattcact tttgaaggac cgaaacgtgg ccgttttgct gcagaaggta tcgctcgttt
    80161 gaaagaaaat gtggatacat tattaattat ctcaaataac cgtttattag aagttgtaga
    80221 taaaaagaca ccgatgcttg aagctttccg tgaagcagat aatgttttac gacaaggtgt
    80281 acaaggtatc tctgatttga tcacagcgcc aggttacgtt aaccttgact ttgctgacgt
    80341 gaaaacagtg atggaaaacc aaggtacagc attgatgggg atcggtgtgg ctagtggaga
    80401 agatcgcgta gtagaagcaa cgaaaaaagc aatctcctct ccattgcttg aaacatctat
    80461 tgatggagct gaacaagtac ttcttaacat cacaggcggt ttggatatga cattgttcga
    80521 agcacaagat gcttcagata tcgtagcaaa tgcagcaact ggcgatgtaa atatcattct
    80581 aggtacatct atcaatgaag aaatgggcga tgaaatccgt gtgacggtca tcgcaacagg
    80641 tatcgatgaa tcaaaaaagg aacgcaagtc tagccgacca gctagacaag cacaaatgca
    80701 atcacctgct caaaagactg tattagatat ggatcaagct aaaccaactt cttcagaaga
    80761 agaaaacagt tttggcgatt gggatatcag acgtgaacaa aatgttcgtc ctagagttga
    80821 tgattccaat tttgacaata tcgaaaaaaa agaatttgat acattcaacc gcgaagaaac
    80881 aaaatctcat ggcgatgatg aattaaatac gccaccgttt ttccgtcgta aaaaataaag
    80941 gagaggaaga gcacatgatt gctgacaact tgcagaaaat caagcaagaa attcaggatt
    81001 cgtgtgctct tgttcatcgt gatccgaaag aagttacatt agtagctgta acaaaatctg
    81061 ttgaatccgc tcaagctgaa gaattggtta gagctggcgt tatgaattgt gcagaaaacc
    81121 gagtagataa attattagaa aagaaacaag atttatttga ttttcctgaa ataaagtggc
    81181 atttaattgg taatttacag cgcagaaagg taaaattgat tgtaaatgaa attgattact
    81241 ttcatgcgtt ggacagttta agattagcag aagaaatcca gaagcggtta gagaaagaat
    81301 tatcctgttt tattgaagta aatgtttctg gtgaggaatc aaaacatgga atttcaccag
    81361 atgagctttt accgtttgtg gaatctttag ctgcctgcac aaaaatcaag gttgttgggt
    81421 tgatgactat ggcgcctaaa gaagcttctg atgagcaaat ccataaagtg tttggtacgc
    81481 tgaaagaatt gcgtgatctt gttcaagcaa aaggttatgc ccatgcacca tgtagtgaat
    81541 taagtatggg gatgagtcaa gactttcaaa ttgcgattga agaaggtgca acatttatcc
    81601 gtgtaggaac agcgctattc aaagacgaat aaaggaggaa caaaaatgcc gcttttaaac
    81661 agaggagcaa ttgctaattt tttcggatta gctgatgagg aagagtacga ataccaagat
    81721 tatcctgaaa caacaaaaaa ggctgtacag ccaactaaaa aaaatacaac acagaatcaa
    81781 tcaactcatt ctgtatctgc tgcgaatact catgaaaaac aaaaaacggc gacttcacgt
    81841 cctgtaacgc atgaaagtat tcagccggct gaaaagaaaa aagtaattga actacatcaa
    81901 tcacctacaa aaactgtagc gaaagaaaga acagcttcaa gaacctctac acaacctaaa
    81961 gcaaatcttg ctggaaaaat cactgtaatg gaaccaagag cttattctga agctatggcg
    82021 attgctaaac gaatcattgc tggagaagca accttagtga attttcattt agtagaagaa
    82081 aaacaagcga gaagaatcgt tgattttcta acaggaacag tttatgcact agatggagat
    82141 atcaaacgtg ttggagatga gatttttctt tgtacaccga ctgggatgga gattgacagt
    82201 tcaaccgctc aatcttttgc agatgcgaat ttgtttgatc tgtaagagga ggaactacga
    82261 tttatactat tgcactatta cttcatttac ttcaacgggc agcctatttc tatagcatta
    82321 tcttgatggt atatgcattg ctctcttggt ttccaggcgg gtatcaatca gggctaggac
    82381 gattattagc taagatctgc gaaccgtata tcagtttgtt cgatcgtctg cctttgagga
    82441 ttggaccctt ggatttcaca attttagggg caatcctgat cttgaactta gccgtacaag
    82501 gtttagcaca gatcgttata tggataactg tccatttagt ttattgattg aagcaacaaa
    82561 atctttattc tcagaataga taggagggga aaattttgga tgcaaatgta taccagcact
    82621 ttcgggcaga tgaacgtaca tttatcgatt cggtaggcga ttggattgaa caggttcaag
    82681 gacaatatgc accctatctg accgaatttc ttgacccaag acaatcctat attttagaaa
    82741 cactgattcg acaaagcagt gatttgcgtt tcatgtttta tggcggatat gaacaggcag
    82801 aaagaaaaag atgtttgatt ttccccgatt attacgaacc tgtagaggaa gattttgaaa
    82861 cagcattgta tgaaatccat tatcctagca aatttgccag cctaagtcat gggaaaatcc
    82921 ttggaacttt agtgggaacg gggatcaaac gctcttactt tggcgatatc atctcggacg
    82981 gtgatcgctg gcaagtattt attgcaaatg aggtggatca ttttgttgct gcacaagtaa
    83041 caaagattgg aaaggttgct gtacgcttgg aaccaatcaa atacacagaa atgattatgc
    83101 cgaaagatag ctggacacaa gaaagaggaa cagttagttc catgcgacta gacagtgtga
    83161 tctctgagat ttataatatt tccagacagc ggtccaagca gctgatcgaa gctggtaaag
    83221 taaaagtgaa ctggacagaa aatacaagac ctgattttgt acttgaacta ttagatatta
    83281 tttcagtcag aggttatggc agagtccaga tccaagaact tgaggggaag acgaaaaaag
    83341 aaaagtacag ggtactgttc ggtgtattac gtaaataaaa taaaaaggtg gtttactcat
    83401 ggctttaact ccattagata tccaaaataa gacattcccg acaaaaatgc ggggatataa
    83461 tcaagacgaa gtagatgatt tcttagattt agttgttcgt gactatgagg aattgactca
    83521 aagaaatcga gaattagaaa aagcagtcaa acattctgaa gaaaaacttg agtacttcaa
    83581 tgaattaaaa gatgctttga accaatcaat catcgtagct caagatacgg ctgataaagt
    83641 aaaaacaagt gcaagcaagg aatccgaagt gatcgttact tctgcacaaa acaaagcaga
    83701 tgagttagtt gcgaacgctg aaaaacgtgc acatcaatta acaactgatg cagaagaaaa
    83761 agcaagaaaa atcttgacag atgcaacaga aaaagcaaga cagcttgcga cagaaactga
    83821 agatcttaag aaaaaaacac gagtattcca tcaacgtatc agcttgatgc ttgagtctca
    83881 attagaacaa gtgaaaagtc cggaatggga tgaaatttta caaccattct ccagctacgt
    83941 gactgacagc catgaagtga tcaaagaagt tttagcaaaa cagcttgaca atgaaaatga
    84001 tactgatgta aactcagaca caggagtcat agaagcacct atgacggcat ttgatacaca
    84061 ggctattgat atctcgatcg tgcctggtga aaatgaaaac gagtagaaca gaaaagcact
    84121 ttgttattct taaagcgagc cgatgagagt ggaagttcgg taagacactg catggtgaaa
    84181 gatcctctac ttgagacctg cttttccgaa attagagtaa gaagcagcgg ctgacctcgt
    84241 tattggtcac agagagtttg cactagatag atgcaactaa atttgggtgg taccacggca
    84301 tgttcgtccc ttgcggctga acatgctttt ttgtgtccgg aaaacaaata aaggagtgta
    84361 tggttgaatg aaaatgaaag aaaccttgca attaggaaaa acaaaattcc ctatgcgtgg
    84421 taatttgcca aaccgagaag gcgagtggca aaaagaatgg gaagaaaatc gaatctacca
    84481 aaaaagacaa gagttgaacg aaggaaaacc tagctttgtt cttcatgatg gacctccata
    84541 tgccaatggt aatatccatt taggacattc attgaataaa atcagtaaag acattatcat
    84601 tcgttcaaaa tcaatgtctg gctatcgtgc accatatgtt ccaggatggg atacacatgg
    84661 attgccgatt gaacaagtat tggctaataa aggcgtgaaa cgtaaagaaa tgtccatggc
    84721 agaatacttg gaaaaatgtc gtgaatatgc actttcccaa gtagataagc aacgagcaga
    84781 tttcaaacgc ctaggagttg caggagattg ggaacatcct tatgttacat tagatccttc
    84841 ttatgaagct gcacaagtac gcgtatttgg taaaatggct gaaaaaggct atatttacaa
    84901 aggattgaaa ccaatttact ggtctccgtc aagtgagtct tctttagcgg aagctgaaat
    84961 tgaatataaa gatgtaaaat ctccatctat ttttgtagct ttcaatgtca aagacggcaa
    85021 agatattctt gatgaagata cagcttttgt catttggacg acaacaccat ggacattgcc
    85081 agcaaaccaa ggtattgctg taaatccaaa atttacgtat gtattggtgg aagccgatgg
    85141 ccgtaagttt gtcgtagcga aagatttgct agaaacagtc caatcagaaa tcggttggga
    85201 aaatgtaaca gttctaaaag aatttgctgg acaagaaatg gaatatatga ctgcaagtca
    85261 tccattctat gaacgtgaat cacttgtgat tttaggcgat catgttactt tagacgctgg
    85321 tactggatta gtccatactg ctccaggaca tggggaagat gactatatcg ctggtagcaa
    85381 atataaacta tctgtagtgt cacctgtaga ctcaaaagga ttgtttacag aagaagcacc
    85441 tggatttgaa ggtatctttt atgacaaagc aaatcctatg atcactgatc tattaaaaga
    85501 aaaaggagct ttgctgaagc tagatttctt tactcatagt tatcctcacg attggcggac
    85561 gaaaaagcct gtgatctatc gggcaactcc acaatggttt gcatctatcg ataaattccg
    85621 tcagaatatt ttggatgaag tagaaaaagt agagtgggtt atcccatggg gtaaaacacg
    85681 tttgtataat atgatccgtg accgcggcga ttgggtaatt tcccgtcagc gtgcatgggg
    85741 agtaccactt ccgattttct atgctgaaaa tggagaagca atcatcacac cagaaacaat
    85801 cgaacatgtg gcaaaattat ttgctgaaca tggatcaatt atctggtttg aaaaagaagc
    85861 gaaagaactt cttcctgaag gatttaccca cccaggttct cctaatggcg aatttacaaa
    85921 agaaaaagat atcatggatg tctggtttga ttctggttct tctcatgaag ctgttttacg
    85981 tcagcgacca gaattatcat tcccagcaga tatgtattta gaaggatcag accaatatcg
    86041 tggctggttc aactcaagta tcacgactag tgtggctatc aatggtgtag caccatacaa
    86101 atcagttatt tctcaaggat ttgctttaga tggtgaaggc cgtaagatga gtaaatcttt
    86161 aggaaacatc attgcaccag aaaaagtgat caaacaattt ggtgcagata tccttcgctt
    86221 atgggtatct agtgtagatt atgaagcaga tgttcgtgtt tctatggata tcttaagtca
    86281 agtggcagaa gtatatcgta aaatcagaaa tacgatgcgt ttcttattag cgaatacaga
    86341 agatttcgat cctaaaaaag atactgtttc gtacaatgat ttacgttctg tagacaaata
    86401 tatgaccgtt cgattgaacc aagtaatcaa agaaatccga gaagaagggt acgacaaata
    86461 taacttcatg catatttacc gtacagttat gaacttcttg acggttgatc tatcgtcatt
    86521 ctacctggat tttgctaaag atgtagttta tattgaagca gaagatgatt atcaacgtcg
    86581 ttgcatgcaa actgttttct atcaaacagc tgtagctttg acaaaattat tgactcctat
    86641 cattccgcat acagcagaag aaatctggtc attcctgaaa gaagaagagg agtacgttca
    86701 gttaagcgag tttcctggtt atgaagagtt tgcaaaccaa gaagaattga tggatacctg
    86761 gactgctttc atggacttcc gtgataatgt cttaaaagca ttggaagaag caagaaacag
    86821 caaattgatt ggtaaatctt tggaagcaaa actaactgtt tatccaaaac aacaagttcg
    86881 tgaaatgtta aaagctttgg atgcagatat tgcacaatta ttgatcgttt caccagacta
    86941 ctttgaagtt atggcagcag atgaacaagt gccagataac gctatggtat ttgatgatgt
    87001 tgctattaca gtggaaaaag cagacgggga aacttgcgat cgctgtcgcc aagttcgtaa
    87061 agatgtagga gtagatgaaa aacttcctca tctatgcggt cgttgtgcaa aaatcgttga
    87121 agacttctat cctgaagctg tagctgaagg attcgaagaa aagtaaaagg gaaaaggggg
    87181 tgtgacatgt tctttgtcac accccctttt gttatcaatc gtttaatttt ccacgttcac
    87241 gataccagtt atctggttgc cagatccatt cgaatccgtc tttttcaagt agttcgaaag
    87301 ctgctttagg tcccattgat ccagctttgt agttgggaaa atccggttgt gtttggtccc
    87361 aagcttttcg aatgacatcc acgatatgcc aagattgagc gacttcttcc caatgagaga
    87421 agttcgttcc atcaccattc aacgcatcta atagtaattt ttcgtaagct tctgggctat
    87481 tttcaaccat ttctgcacta tttctaaatt ccaatttgat tggttccgtt tcaaatcctt
    87541 gccctacttc tttcccattc aaggataaag aaaatccttc agttggctga atatagatcg
    87601 ttagaacatt aggtggaagt gtcgtatcgt cacatggttc atcgacagat gttttgaata
    87661 cattaacagg aacttgtttg aatacgatgt tgatccgagt ccctttttca gtcaaacgtt
    87721 tgcctgtacg tacatagaaa ggaacacctg accaacggaa gttatcgata gtgaatttgc
    87781 ctgcgacgaa tgtttcagtc tctgattgag catccacttt gtcttcttcg cgatagccct
    87841 tgaattgtac accatccaat tcaccttgag catattgccc gcgaacgaag ttttctaaca
    87901 cttcttcttc gttataaatg cggatagctt tcaatgcttt gatcttttct gtacggattt
    87961 ctttttcgga aaatgctact ggcggttcca ttgcaagtaa tgctacaacc tgtaaaatat
    88021 gattttgcac catgtcttta agtgcgccac tatgatcgta gtagcccccg cgatcttcta
    88081 caccgaggtt ttcgccaaaa gtaatttgga tattgtcgat atagcgattg ttccattgag
    88141 cctcaaaaat attattggca aaacggatag ctgagatgtt ttggatcatt tcttttccta
    88201 aataatgatc aatcctgaaa atatcttgtt ctgggaatac ttctctgatt tgctggttca
    88261 attcaaaagc agactggtaa tcagaaccga acggtttttc tatgatcaaa cgattgaagc
    88321 cattttttgt cataatattt tgtgatttca aatgagaaac gatcgttcca aaaaattgag
    88381 gtgccatagc caaataatag acacggttac cgtttaattg atagtgtcca tctaatcggt
    88441 cagacaattc ttttaatgtc tgataatgcg ttgaatcatt tacatcatgg gattgatagt
    88501 aaaaatggct tgcaaaggta gttgcttctt catctgaagg attcaatgca cggattgttt
    88561 ctttgacaac ttcacggtag tgctcatcgc tccagggacg gcgagcggtt ccgattacag
    88621 caaaatgctc gcctaaattt ccttttctgt aaaggcggaa caaagacggg tacaatttac
    88681 gctgggctag gtcacctgta ccgccgaata tcgtaaacaa tacatttttt tcattagaca
    88741 tgtggataca ctcctttata ttttatcgac aaaaatcgtg ctggccgcct taaagctgac
    88801 ggggattttc tttctatcat tttggatagt gatcggtcct tcataagctg cgatgtcaac
    88861 gatctcatat tcttcgttga tattcaggtc aatagtgact aagtaatcca acaattcttt
    88921 ttcatcaagc actcgtgcga tacgcacttt tgtaccgaca ggataggact ccaatgtttg
    88981 tcggcgttct tcatggacaa cttcctcttt tttaggaatc gatcctccat gaggacaatg
    89041 ttcaggatga ttcaaatagt tttctaaatg atctgcaaga tgttccgaag tcacatgttc
    89101 caatacttca gcatcatcat ggacttcatt ccagctatag tctaaatgtt caactaaaaa
    89161 aacttcccat aagcgatgtt tgcgaatcag actgctagct ttttgcagtc cgcttgcagt
    89221 caattgtaca ccttgataag gggaatgttc tacaagctct tctttcacaa gtttagaaat
    89281 catttcactg acagatgcag ctgagacatc tagactagat acgatttgtt tattgttgac
    89341 tttcgttgta tctccaccta gttctaaaat gattttcaaa taatcttctc gatttggtgt
    89401 catattcgat catcctttca tggtaaaaaa ccatcattag taagtgtaac aaaaaaaacg
    89461 ttaattgact atttttctaa ataaaaaaca aaggattttt agacgttttt acaacaaaaa
    89521 acgaaagatg atagagaaga ctcattcttt cgtttataaa ggatatatat atacattggg
    89581 agggatctat ttttcagata acaacttata tgaagtgtct gcagattcgt cataaagagt
    89641 ttgttcggga taagaaaaga taagataaaa accatactga tcacgtacga ctaaacgata
    89701 ttcatctatt tctaccacag atgtgccaag agactgaccg ttgatcaaaa acgtgtaatc
    89761 agatgtaatc tgtaattgtt gtttttgctg gctatcacta aagaaccatg taccttgata
    89821 ctttgagagt ccttctgact tttccgcttg ttttttcttt ttcaagtaaa taaaacttga
    89881 gagaacggta atgagtgaac caagaaaata gatcattttt tttatcacca aatcactcct
    89941 ttatgagaaa ataatcgcat agacatattc tttcttcttt atttcattgt accgaataaa
    90001 agctaaaatt tctctaagac tttcgactgg tttttttact gtttttctac aaattatgat
    90061 aggatacata gtaggaagaa aggtgacata atttgaagta tcagcaagtg gaaatagctt
    90121 tttttattga ccgcccgaat cggtttatag cacattgtat gaaaaaagac ggagaaataa
    90181 ttattgcgca tgtcaaaaat acaggtagag gaaaagaggt cctgatacca ggcgctgaag
    90241 tggctattgt atttgctcct ggacctaaac ggaaaaccgc ctatgatctg attgctgtaa
    90301 aaaagaaggg tgactggttt aatatcgata gtcaattgcc taatcggctg gcaattgatg
    90361 gtattttaga cggcacgatc cttttaccag gattaactgg tccatttacg gtatacaagc
    90421 gtgaagtaac ttatcttcac tccaaatttg atatttattt agagacggtg gtcggacaaa
    90481 aagcatttgt tgaagtcaaa ggtatgacac ttgaaaataa agcgattggt gcttttcctg
    90541 atgcgcccac actcagagga ttgaaacata taggagaatt aacgtatgcc gcacaagatg
    90601 gttatgccgc ttatgtcttg tttattgctc agttcgaaca tcttcatctg gcaacgatcc
    90661 atgaagaaat gcaaccagca ctagctgata tggttcgaca tgcgcagcaa agcggagttc
    90721 aaatcctcgc ctataattgt caagtatcgg cagatgaggt aactgtcaag caggccattc
    90781 catttgatgt agatatccct tttgaagatc ctatacaata gaaagaaggt aaaataatga
    90841 tcaacttagg tattatcgga acaaattgga ttactcatca atttgtccaa gcagctttaa
    90901 gcacaaaaaa atatgaattg acagcggtat attctagaca tttggaaaaa gcacaggaat
    90961 ttggttctca ttatgaaggg gatatcgaat tcgcaacaga tttgaagact tttttcaata
    91021 ttactcatat ggacaccatt tatattgctt ctccgaatag tcttcacttt agtcaagcta
    91081 agcaagcaat tttggcagga aaaaatgtga tagttgaaaa gccagctttt tcgacgccaa
    91141 aagaaatgga tgaaatcatc catttggcaa atgaacacaa tgtgtatttt tttgaagcag
    91201 caagaaatat ccatgaatca ggttttaaaa agattgccga ttacttacca ttaaaagacg
    91261 agataatcgg tgcaaatttc agctatatga aatattcttc gcgctatgac caagtattag
    91321 atggagaaga accgaatatc ttctcgactc atttctcagg tggtgcattg atggatttag
    91381 gtgtttacct tgtatatgca gctgttgctt ggtttggaat gccaaaagaa gtccattatt
    91441 tcccaagaaa gatttctact ggtgtagatg gtcttggatg ggggatctta cgttatgaga
    91501 attttgatgt atcgatccag ccaggtaaaa tcggtgattc ttatttaccg tcagagattt
    91561 attttgactc aggaacgcta gtgatgaatg gagtcaatgc aattgaacaa gcacaatttc
    91621 atgatcgcgc gcataaagaa atcattgatt tggcggtaac agctgcagaa aatccaatgg
    91681 cagatgaagc agaggatttt gccaatgtca tggctcatcc gaaagatcca gcttggggat
    91741 tgttgtatga agagtggatc gagctagcta gaaatgtgaa ccaagtgatt tatgatttaa
    91801 gaaaaaatgg cggcatcaaa ttcgatgcag ataatgagga agatttttaa tgaacgaatt
    91861 tgtacaagta tttcccgaaa attggcagaa aaaatatgag gataaagggt ttatctcacc
    91921 ttctcttatt caacgagcag tttttcagcc actaagtaat aaacaaagta tagtagcggt
    91981 ttctcctaca ggttcaggta aaacactggc ttatctttgg cctctactac taaacgtgga
    92041 accaggtgaa gcaagtgcgt tggtcatttt tgcttcttct caagaactag ctattcaagt
    92101 agcagatgtt gcacgtgaat ggggaaaaga caaagagttg aaagtccaat ctttggtggg
    92161 cggagccaac gtcaaacgtc agattgaggg tttgaaggaa aaaccagaaa tcttgattgg
    92221 tacaccagga agaatattgg aattgatgaa agcaaaaaaa ataaaagcac atcaagtgaa
    92281 aacaatggta tttgatgaag cagaccagct atttgacgct gggaacagcc aaatcattga
    92341 tcaaattctt catcaagctc caacagagta tcagctagct tttttttcag cgacagcaga
    92401 tcgttccctt gaaagcattg aaaaaatcac tgggaaaacg tttgagacaa ttgacgtgac
    92461 agcagaggac gattctcgaa aaggactgag acattacttt ttacgtgtac cgatacgaaa
    92521 gaaagatgac tacttgagac gtctcacgca tatagaagat tttcatggtc tgattttctt
    92581 taatcaactg actgaattag gcgtaatgga agaaaaattg ctgtatagag gtgtaccggt
    92641 aggaagtctg gcctctgatc agaacaagct actccgaaaa gcagcgctag aacaattcaa
    92701 aaagaaaatg gtcagagcac ttttgaccac agatgtggcg gctagaggtt tagatattac
    92761 aggtcttcct tatgtagtga atgcagaagt tccgttaagt gaagaagctt atttgcatcg
    92821 ttcgggaaga actggtcgaa tgggtaatga aggaatagta attacactcg tacaagataa
    92881 taatctaaga gatttaaaac ggcttttacg tccatcaaat ataacacttg aagagatttt
    92941 tttatacgga ggtaagctgc aaactgagca gccaaccaaa gaagatgcag ctcctgcagt
    93001 aaataaaaag tatgcgaaaa ctttttctgg aaaggagcag aacccagaat ataacaaaga
    93061 aaaaataaga aaaaaagtta aaaataaatc aaaaaaagag agaaataaag gagcaaggcg
    93121 caaataagcg cctttttatg ctatgattat gaaaacggga acaaaattgg ttgccagagg
    93181 aggggttatg tgaaagaacg tcaagaagag atcttgcagc tattgacaga aaaccaacaa
    93241 ggattgacag caagtgatgt agctgagcgc ttaactatcg atcgaagcaa tgcaagccga
    93301 tatttaagtg aactatacaa ggcccatcat attgtaaaaa ccgctggccg accagtcgtt
    93361 tactctttgc caacggaaaa gtcaaaatct gatgaagtgc atgtcgactc ttctacgcaa
    93421 gtgacttttg aaacactcgt tggagaaaat gattctttaa aagtcagcat acagcaggca
    93481 aaagctgcga ttctttatcc accgcggggg ttgcatacga ttatttttgg agaaactggt
    93541 acagggaagt cgatgtttgc tgaatgtatg tatcattttg caatcgattc agagatgctt
    93601 tctgcagatg cgccatttgt ttctttcaat tgtgcggatt atgctcaaaa tccgcaactg
    93661 ctctttggac acatctttgg gatcaaaaaa ggggcttaca caggagcagc tcaagatagt
    93721 cccggtctaa tagccaaagc agacggcggt atattgtttt tagatgagat ccaccgttta
    93781 ccgccggaag ggcaggaaat gctgtttact ttcattgata aaggtattta ccgtccgctt
    93841 ggagaaagtg cacaagtaca tgaagcatct gttcagatca ttggggcgac gactgaatct
    93901 tcagaaagtt tcttatctac gttcaatcga cggattccta tggcaatcac cttgccgaat
    93961 cttgcttcac gttcactgga tgagcgttat gaaatcatct ctctttttat caaacaagaa
    94021 gcgaatcgtt tgaatcaacg tatcgatatc gaaaaagaag cgattcttgc cttcatgctt
    94081 tatgatgcgg aaggaaatat cggtcaagtg aaacgagact tgaaactagt ctgtgcaaaa
    94141 tcttttttac attaccgtac tcatcatgag aaaaagctaa tcattcgaaa agaagaactt
    94201 tcccttcaag tacaaaaagg attactgaaa atcaaggaaa taccggaacg tctcgaccga
    94261 ttcatggaca gcaaaagtca gtatttgaca tttgaaccag gatttgctga tgttgtctgg
    94321 tcacaagatc cagaacgaaa catgcaggtc tataacgata ttgaggaaaa agtcacttct
    94381 ctttccgaaa ccggtgtgga gaacattgat ctagagaccc tcatttcaaa agatgtggat
    94441 gcttatttcc agacatatgt caaagaattg actaaaagtt cgattcccaa agatcttttg
    94501 ccagatgaaa tctggcggtt aaccaatcaa ttgtatgatg tggctgaaaa acggttagac
    94561 cgtatttata atgaaaaagc tcgttttgct tttgctttgc atctgcaaag tacattggat
    94621 cgtgtgagag aaggtcatat gatcgtccac ccagatttga ataacgtacg gaaaaatttt
    94681 aagagggaat tccaggtagc aatcgatctt tcaacgatca ttgaagaaga agaacaagtt
    94741 gagattcctt ttgatgaaat cggctttatt agtatgtttc tttctatcaa tctaggagaa
    94801 agtgagcctg ttaaagaaaa caaagtagat gttctcatat tgatgcacgg cagacagact
    94861 gcttcaagca tgttggagat ggcacaagaa ctattaggaa caaaagtagg gaaagctttt
    94921 aatatgccac tggttatgga agttcaaaca atgtatgaag aagtgttaca atatgtcaaa
    94981 aactatcaag agcatttgac gaatggccta ctcttgttaa cagatatggg atcattaaac
    95041 accttcagtg atttgattta tgaagagact ggcattcgga cgaaagctat cacgatgaca
    95101 agtaccatga ttgtgataga agcgatacgg atggcaagtg ttggaagaag cttagaagat
    95161 atttatcaaa atatccagct ttcctttgaa tcgatcgtac gtgatcagtt caaagcggaa
    95221 aagtctgaaa ctttacagcg taaaaaagcc gtaatcgtga catgctttac tggtgaagga
    95281 gtagctgcca aattatatca gcgtattatg ccagtgatta atcaaaataa ggtagaactg
    95341 atccagatgc agtttttaga acgtgaatca ttcaaaaaac acatcgatgc cttattggaa
    95401 gaatatgaga tcaaagcaat tgcgggaaca gttgatgttg attatcaaaa tatcccattt
    95461 ttttcagcct atgacgtttt tgatgatgaa aaactaaata ttttaaagcg tatagctgat
    95521 gacgagatcc cgacagctaa aattgtcgat tcacttgctg gatcattaag cgatgtcttt
    95581 tctattgatg aactgatccg ttcattgcaa aagacagttc agcagatcca gaccgatttg
    95641 tcgattatcg tggaacctgg agtagatact ggaatcgtta tccatttagc cttcatgtta
    95701 gacggattga tcaaacaaga aagccatcgg caatttgaga aactgtctat tttctcaaaa
    95761 atttatcgtt tggaaatgga tatcgttcgg gcaaacttga tggcactaga acgaaaatat
    95821 caggtaaaaa ttccagaaga tgatatcgca tttcttacac aaatgtttct tgaaaataaa
    95881 ataaaaaaat gaacacacta atcatactgt ttacacacta aacatctgtg tgcgcactat
    95941 aaaaaagagc taaaaagcat gaaaacgcta tttcagctct tttttttatt ggcatgcttc
    96001 ttgcgttata taaaagtgta aaaaaataaa aggagtgtta aaaatggcaa aaaaaacaat
    96061 catgttagtt tgttcagcag ggatgagtac gagtttattg gtcacaaaaa tgcaaaaagc
    96121 agcagaagaa aaagggatgg aagccgatat ttttgcggtc tctgcctcag atgcagataa
    96181 taatttagaa tctaagaatg tcgatgtatt attattaggt cctcaagtac gtttcatgaa
    96241 agctcaattt gaacaaaaac ttgcgcctaa aggtattcca ttagatgtca tcaatatgca
    96301 agactatggc atgatgaacg gagaaaaagt tcttgcacaa gcagaaaatc taatgaaata
    96361 aatcaagtat gcagttgggg gtataacaac gtggaagatc aacaaaatct agaagccatc
    96421 atggggttaa tcatgtttgg cggtaatgca aagagcgatg caatggaagc gattgcggct
    96481 gctaaaatcg gtgattttga gttagcggat aggaaaatcg ctgatgcaga agaatcactt
    96541 atacaagcgc atcatgcaca aacagacatg ttgacacaag aagcacaagg aaatcatatg
    96601 caagtgacat tattgactgt tcacagtcaa gatcacttaa tgacatctat tgcgtttact
    96661 gatttagcaa aagaaatcat cgaactttat cgtcgcgtat acagcgaata aatacaactt
    96721 cttttttgaa cggaagaaga aaaatttttg aacggatttt ttattttgtt ataaggagaa
    96781 ctctctttat ataaaaaact tataggagga agtaaaaaat gaacggacta tcaagttgga
    96841 tggagaaata catcctgccg gttgccgcaa aaatcggagc gcaaaaacat ttagttgctt
    96901 tgcgtgatgc ttttatcggc acactgcccg caacaatggc gggatcagtc gctgtaatga
    96961 tcaatgcaat tattcgtgat ttaccgcaac agtttaacag tacctatgct gacagtttag
    97021 cagcagacaa aggaatcttt gccgtattga atgtcatcat cggaatcaat ggttttgtct
    97081 ggaacggtac actagcaatc gccggattga tctttgcctt ttcttggggg tataatcttg
    97141 cccgagctta tggagtaaat gatctagctg gggggatcgt tggtttagct accttgattc
    97201 aaggtatttc ctttggtcca gcattatcag cagcattaga tgtgaatgtt ccttcaaatg
    97261 tcgcagatgc aatcaatagt tctgagattg gtgcaacgat tgcagatggc acactgtcag
    97321 ttggattatg gggttggcta aaactagatc atttgaatgg aaacgcttat tttacagtta
    97381 tgatcatggg agctatttca gtcattattt tttgtaaatt gatgcttgcc aacattacga
    97441 tcaaaatgcc tgattcagta ccaccagcag tatctaaagc atttgcagcc attttaccag
    97501 cgacgatcgc tttatacgtc attgctatta tcaattatat cgtaggtcgt gtagctgatg
    97561 ggcaactgat cattgatttg gtgcaaaaat atattgcaga gccattccta ggattatctc
    97621 aagggttggg agcagtattg atcgtcacag tatttgttca agtattctgg ttctttggta
    97681 ttcatggacc aaatgtattg gcaccagtgc tagagggaat ctggggacaa gcacaactaa
    97741 tcaatattga tattttccaa aaaggttata acggaaaaac tggtacacca gccgtgttag
    97801 atgcaatcga agatggaaaa gcttatatgt gggtccgcgg atcgttcgat gcttttgcat
    97861 ggtttggcgg atcaggcgga acgatcgtct tactgatagc tatcttgatt ttctctaaac
    97921 gtgcagatta tctaacagta gctaaattat ccattgggcc tggtatcttc aatatcaacg
    97981 aaccagtgat gtttggtctg ccaatcgtat tgaatgcaat catgtttatc ccattcttga
    98041 ttgctccagt agtagcaaca gcaattggat ggacagctac ttatcttggc ctagtcgctc
    98101 cggtatcgca gcaagtcaca tgggtagtac cgccgttctt attgtcattc ttagcaacag
    98161 gagcggattg gcgggcaccg atcgtaacat tagtatgtat ggtcgtgact ttcttgattt
    98221 gggcaccatt tgtcatctct gcaaacaaaa tggaccctgc cgcaggcgaa caacaataag
    98281 atgaacgtga ggagcgggac gctttaagtt tcgttcctca taatgaattt tcgaggagga
    98341 actgaaatga ttacaattgc cagtgcaatg caacaagatg aaatcaaaga attgttagaa
    98401 ggatacaatg aagaaggaat gacttttaca tttaaagaaa aacaaggaat caagctgttc
    98461 tttgaaacaa gtgcagaaga tccggaagtt gcggctaaaa aagcaaagga actaatcaaa
    98521 gcacagcctt ggggaacagt cctttatttt caatcaacag ctgtttagaa aatggtttaa
    98581 aaagatagga tggttgagat gataaaaaac ggtttatttt tatgtagtat tggttctttg
    98641 atcgtgttgt atagtttgaa tccacattcg caaaggtatg atttgtatag tatggtcaca
    98701 ggtctttttc taacaggatt aggaatctat ttcttcatga aggggaaaaa aagagaagaa
    98761 gcaaaggaga aaaataacta atggaaatca cgaaaaaatt aaatattcca gcatcgtttt
    98821 tttacgataa agttatggac tctgtccttt tcgatgtacg taaagcgacc ggcaaatcag
    98881 taactagaaa acagctgaaa aatttggagt acgtcaaaca attctcgcaa aataaccggg
    98941 ctcgcataaa aatcgaagaa gtcattgaaa accagtctta taagtttcgt acatcgacaa
    99001 cgaagaatga attcgtcgtt cattatcaaa tcaaaccatt ggacgattcg acctgtgagg
    99061 ttcattacac agaaaaaatg gagtcttatg gcatgcttca aaagttgaat gatatgcttt
    99121 taggaacaat cttgatgtat tttaaacgga gacgtttcaa aaaaatgcta gatatgatcg
    99181 aagcaacgta ttaaatcaat gaaacatggt actatttagt tattgaaaaa tgaagtaagc
    99241 tagaaggaat ttttcatagg aagatagatc tttcctgcga atcctcacta gcttttttgt
    99301 aggaaggggc agcaaaatga caaacaaagt aagagagcta ggtgtatctt cgattgagga
    99361 aatgtttgat ttagccactt atgcatttaa tggggcagat acacctgaaa ggcgagaacg
    99421 ttttcgtaca cttgcggaga attcttggaa ttacggattt tttgatgagg aggatcggct
    99481 aacaagtcaa gtcatggcga ccccttttcc agtgaatttt tacggacaag agtacctaat
    99541 ggccggcatt ggctatgttg cttcttatcc agaagcaagg ggacaaggcg ggatcaatca
    99601 gatcatggaa aagatccttg aagattgccg tgatcgtaaa gtaagcttat cttatctggc
    99661 gccattctcc tatcccttct atcggcgcta tggctatgaa caatcatttg ataaaatcag
    99721 ttatcaacta aacagtcgtg atgtgccgtt tatcaaaaaa aaatctggaa aaataaagcg
    99781 tatggatttt gaagatgcaa aagctgcaat cagaagaatt tatgaacaga tgccggaaaa
    99841 ccaacgagct ggtctgaagc gcgaagactg gtggtatacg tataaattca agcaaaaagg
    99901 caattaccag tttgctttgc attttgatga taatgaggaa gcaacgggat atatcgttta
    99961 tcagcttgca acgtcttcct ttattattgc ggagtgggga tttttggatc atgatgcttt
   100021 ttgcagcctt gttcgcttcg taagttctca caatggtgct tttgaaacat ttgaatattc
   100081 atgcggctac ggcggtaatg atcaaaatta tctattagaa aatccatttg cttctgtaat
   100141 gatcacacct tatatgatgg cgaggatcgt agatatttct ttgtttttgg aaaattatcc
   100201 atttaaaaag agacaagctg catttgccct acaaatagaa gaagatcatt atgcgaaatg
   100261 gaatgaagga atcgtggaag taacaatcga aaatggaaaa aatcatctct caaaagtaga
   100321 acaaacaaaa ctcccaatcg ttcgaggaag tatccaacat atcacgcagc tattattagg
   100381 gtatcgtaaa gtggaagagt tggtattcca agaaagaata caggtgacaa aagaatatga
   100441 agaaacgtta agctcaatct tgccacagca acagccaatt cttaatgact atttttgata
   100501 aaaaacgggt gatgacaata tttttgtcac cacccttttg tttttcattt ggcatttgaa
   100561 ggaaaatgtt aaagtaaatc ctcttttttc gtaaatttga attttttgtt ttcttttgtt
   100621 ttagggtttt cttttttgtg gtcttggatt gcttctttcg taatcttttc ccgatttttt
   100681 ttagatgaat ctaagcgttc tgcgtatact ttttcttttg gcttatcatt cattggaaag
   100741 gacctcccta cattatttca ttctacctct agtttatcat ctgttaagga ctagtggaaa
   100801 cgttttgcct aacttataag ctgatttttt tagcagataa gcttgcaagt aaaaaacaaa
   100861 caagctacaa ttgatttatt gaaattgttc ctgtagctca gtaggataga gcaatcgcct
   100921 cctaagcgat aggtcgctgg ttcaattcca gtcaggaacg tacaaataac ccctgtatcc
   100981 tttgcggtac agggggttat tttgtataag tagacattgt agtttctaaa ctgctatgtc
   101041 ctaaacgttt cgatacgcta agtatattta ctccttgata aagtaaaata gatgcatgcg
   101101 tatgtcttag tccatcaata aacagtcgaa gagaaaatag ggaaaaattg attactaaag
   101161 aaaaacttac taggtcgacg gtgtagttga ttttcatact tcaatagatt cgaattaaac
   101221 gatttcgatt ggcttatacc agtcctatcc aacgaaagat atatcgttct taactctagc
   101281 ggtataactt gaagctttac gtaaagccaa tgggaatgaa ctggagatga ctttttatta
   101341 tttttagtaa tagctgatgg gttaatactt acaactttcc ttttcaaagt accatataaa
   101401 aaaacggtgg ggttgtttag cgaaataaag gatctaagcc aatagcttag atcctttatt
   101461 ctatgcttta tattattttt tgcatgtgtg cgaaagtata ctcaagccca atcgccattt
   101521 cggaaaatcg gtactgtaga gccatcttca cggattccgt cgatatccat cttgtcagat
   101581 cctaccatga aatctacgtg ggtttgactt ctattcaatc cagcttctgc taattcttca
   101641 tcggacattt ctgtaccgcc tttcacactg aaggcataag cagaacctag tgctaagtga
   101701 ttcgatgcgt tttcatcaaa taatgtattg aaaaagatga tgccagattg agaaataggt
   101761 gaagggtcag gtacaagtgc gacttctcct aaacgacgcg caccttcatc tgtatcaagc
   101821 agttttgcta agacatcttc tccttgttcg gcagaaaaat caacaacttt tccatcttta
   101881 aaagtaaatt tcatacccga gatgatggtg ccagcataac tcagtggctt tgtacttgaa
   101941 atgtaaccat ctactcgatg actgtcaggt gctgtgaaca cttcttccgt cggcatattt
   102001 gccatgaatt tttctccacg agcattatag cttccagcgc cttcccaaag atggttttta
   102061 ggcaatccga taatgatatc tgtaccggga gcagtatagt gaagggcaga aaattgttct
   102121 cgattcaatt cttctgcttt ttttgctaat ttttcatcat gcttcttcca tgctaagaca
   102181 gggtcttctt catagacacg agtcgttttg aaaatctgat cccataatgc ctcaacttgt
   102241 ttttcttctg gcagttctgg gaagactttt gctgcccatt gtttaccagc cgcagccacg
   102301 acagtccaac tgactttatt tgcttgagtt gccttgcgta aattcattag tgctttgcca
   102361 ttcgctgatt gataagacgc tactcgatgg ctatctacac cagcaaatgc atcgggatcg
   102421 gcagaaacaa cgctgatcct gcttgctcct ttttcaagcc attcatccgc ttgatcgatt
   102481 ttagattgtg gtacattctc aatacgatcc gttgcagcat gtaaaagaaa ttctctttgg
   102541 atctggtcgt cagtccattg aacgataacc tcagcggctc ctagtttata agcttcttgt
   102601 gtgatcaatc gagcaagggg tgcctgttcc acgctgatct gtaaaacaac tgtgtgtcct
   102661 ttttctgtgg ctacgccagt ttctgcaatt aagcgtgcat atttttttag aagttcatta
   102721 aaatcagata acataataaa aattcctcct caccattttt tctggtaact attagcatga
   102781 taccatttaa ttatgattca aacaatgagg ctttaaataa aaaaaggagg ttattttttt
   102841 aaggttaagt ttcatgatat atgttactac ttgctatttc agcttgtgaa gaaaatcaaa
   102901 aactaatttt gcatatcaaa aaaacatgtt acaatgaaat taatcaatgg tggaagcgtt
   102961 ataaaaaaat taaggagatt aaaaaatatg gcaagaaaaa agacgattac tagagaacag
   103021 attttggcag ctgcttatga agtagctgcc accgatggat ttacacgatt tactgcgaga
   103081 aacatcgcga acaaaatgaa ttgttccacg caaccgattt atcttgagtt taaaaatatg
   103141 gatgacttga gacaagctct ttttgagcag atctatgatc atttacaata tgaagtgttc
   103201 actgttgagc atacagggaa tacgattatc gatcttgctt ttaattatat ccattttgca
   103261 agaagagaga agaagttgta tcgttcactg tacttagaag aatatggtgg aggaaaagag
   103321 atgcagaatt tctcttatac ttactttgtg aatacagtga aacgcgatcc agcttatgtg
   103381 aatctttcag aagagcagat cgaatctttg tataatggga catggatcat agctactggg
   103441 atcgcagctt tgatgtcttc aagcatcatc catccaactg atgaacagat tgaaaagatg
   103501 atccaagaca gtatagatgc tatcttgaaa ttcaaagaac caatcgaaat cgattgaata
   103561 tactcaaaag ttccacaaat cagtaggtaa caagagatgt gtcaaaataa ggaaaagttc
   103621 gtttttctta cagcgtatgc atttttaact cccgaagaac tcttccatga atgcaatgaa
   103681 taaaagagcc atacagattg aataaatctg gatggctctt ttattgcaag tatattaaat
   103741 ttttgcgact agttctttag cgaaagcttc taaattttcg atatcttctt cttcagcatt
   103801 cagatccact ttaacatttt cggctccttt tgatgcgcct gtttttgcaa aagcagcatc
   103861 gaaatcatcc accgatttgc agaattcatc gtaaaaagta tcaccagatc cgcagacacc
   103921 gtaaattttt cccaatagat ccaattcttg caagtcttca taaaagtcta cgatctcatc
   103981 aggcagatcg ccatcatcgt atgtataagt agctactacg caaatatctg cctcctcgaa
   104041 ttcatctgca tctacttggg tacattcgtt gatttcgact tcgatatcca gattttcaaa
   104101 tgcttctgca acaatatcgg cgatttcttc agtgtttcct gtcatgctag catatacgat
   104161 ttttgctaaa gccatgatgt gcctcctcgt ttataattat ttaacaatta tgaattatac
   104221 caaaacatca ttgaaaagaa aataaaacct aaagacacct ggggaatgtc atttggctaa
   104281 atgtatgccg ctatgttaaa atgagaggga tttgtaaaaa ggagacgaaa atatgattac
   104341 tttaaaatca gcacgtgaaa ttgaaatgat ggcagaatct ggagctttat tagcagatgt
   104401 acataaaaat ctgcgtgatt ttataaaacc aggaatcaca agttgggata ttgaggtatt
   104461 tgtcagaaac tatatcgaga gtcatggcgg aattgctgcg caaattggct ttgaagggta
   104521 tgaatatgcg acttgtatca gtataaatga tgaaatttgt catggattcc ctcgtaaaaa
   104581 gcctttgaaa aacggtgatt tggtaaaagt cgatatgtgt atcgatttga aaggcggcat
   104641 ttctgattct tgttgggcct atgtagttgg agaatctaca cctgagatcg accgattgat
   104701 ggaagtaacg aagaaagctt tgtacatcgg tattgagcaa gcacaagtag ggaatcggat
   104761 tggtgacatc ggtcatgcta tccaaactta tgtagaaggc gaaaatctag cgatcgttcg
   104821 tgattttatt ggtcatggcg ttggtcctac gattcatgaa gaaccagcag ttcctcatta
   104881 cggagaagct ggaaaagggt tgcggttgaa agaaggcatg gttatcacga tcgaacctat
   104941 ggttaatacc ggaacttgga aaatgaaaat ggatcctaac ggctggactg cgtatacaag
   105001 agacggcgga ctttcttgcc aatatgaaca tacattggct attactaaag atggtccaag
   105061 aattttgaca tcacaaggag aagaaggcac ttattgatct atccagtcaa taggtacggg
   105121 agaggtacat gaaagtttgg aacaaaataa aacaaaatga aaatctgatg cgctttattg
   105181 agacgactca aaagcgcatc actgattcag aaatgagtac gacctctgtc gtcgttgctt
   105241 attatttact cttgtcttta tttccattga ttattgcttt tggtaatatt ttaccgtttc
   105301 tccacatcga tcaagagact gtactgacct atttacgaga agtgattcct gagaccgttt
   105361 accagtttat cggtcctgca atcaaagacc ttttgacaca aagttcagga ggcttgttat
   105421 ccgtttctgc attagcggct ttatggtctg caagtcaaag tatcaatgct ttgcaaattg
   105481 cgatgaataa agcgtatggc gtagaaaata gaaaaaattt tttgatcgtc cgtttcttct
   105541 cactaattgt cattgtcttg ttcatgattg ctattagtgg ggtaactatc gttcttgggt
   105601 tgggacagct gattttggat gctattcagc caatcgtaca aatacccgtg aactttatcg
   105661 atcagttcca aactttgaag tggccaatca cattggtagc tttatttgtc atcatgttct
   105721 tgatttattt aattgtccct aatgctcagt tgaagttgaa gacgatcatt ccgggcacga
   105781 tcttcgctac tgtcggctgg atgctgttat cacaagtgtt tagtatctat gctaggtatt
   105841 ttgcaacgag gatcagcggt tatcagatca taggaagttt tattgtactc atgctttggc
   105901 ttaattttgc tgctacgatc atcattttgg gcggaatcat caatgcagtt gttcaagaat
   105961 acgtgacggg tcatcaaatc aaaaaacgtc gagatccaag ccgaaaatgg ttcaataaat
   106021 tgaaaactaa attttctcat aaaaaaaagt agaaacaaga gttcttattg taaatttata
   106081 tcagtaattt ataataatta tgactcttaa ttaaagtagg tgcccatatg cttgtttctt
   106141 ttagtatggt tataagattt tttctgacaa ttttattatc actgatatta acacctgtat
   106201 tcaaaatcat ttcagtacaa acaggtatgg tcgacaaacc aaatgaaaga agaataaata
   106261 aagttccaat gccttcagcg ggcggactgc ctatctttat ttcatttgtt atatcgactt
   106321 tattcttatt ccgtaaaatt atccctcaat cgtatattgt accgatcttg attgcttctt
   106381 tagtaatcat agtcacagga gtgctcgatg ataaatatga gctgacacct aaacaaaaaa
   106441 gtatagggat cttgattgct tctcttatta tttattttgt agcagatata cgtattgatt
   106501 ccttcacgct tccttttatt ggatatatcc aactaggctg gcttagtttt cccgtaacga
   106561 ttttttggat cttcggtatc acgaatgcgg tcaatctgat tgacggtttg gatgggctgg
   106621 cggcagggat ttcattgatc ggtttaatta cgataggaat cattggatat tttttcctcc
   106681 atgcttctac agtgtatatt ccagttgtta ttttctgtct tgtagcaagt attatcggtt
   106741 tttttcctta caatttttat ccggcaaaga tttatttagg cgatacagga gctctattcc
   106801 tagggtttat gatggccgtc ctttcactac aagggctgaa gaacgtgact tttgtttcat
   106861 cgatttcttt gctgatcgtc atgggagtgc cggtaacaga cacgtttttt gctatcatta
   106921 ggagaaaagc aaaccgtgtt tctttttcta cagcagataa aaaacatctt catcaccgac
   106981 taatcagttt aggctttact cataaaggtg ctgtcctgac gatttatgcg atggctttga
   107041 tgttttcttt cacagctatg gtcatgaatt atactggaag aatcggaacg atcgtattga
   107101 ttattgccat gttgtttgct gcaattttat tatttgaatt gattggtctg atcaatgaga
   107161 aacatcagtt tattttaaaa acattgcgat ttttagggaa taaagagtac cgtacgcaag
   107221 taaaacagaa atatttgaag aaatggaacc gtcatataga tgaataacaa attaaaccag
   107281 ccgatcctta ttagtattgt gacggcaaac agtgataaaa tttttcggac actagataat
   107341 tttatttcaa ctgtaaaaga tgaaacagca gtaaaaatcc gaatttttga caacaattca
   107401 cttcccgagt acaaaaaaag actaacagag tataggaaat atccctacat cgatatctat
   107461 tttcatgatg aaaattccgg atttggttat ggacataatc acaatctgat agctagcgat
   107521 tctcgctatg gcattatttg taacccagat attttagtgg atgaagagac agttacagca
   107581 ttggtcttgt tattgaaaga acatccggaa tgtgcgatgt tagcacctaa gattcttaat
   107641 gaagatggaa caactcagca cctcatccgt gagcagctgg ctgtttttga ttacgttttg
   107701 cgctttgtgc ctttcaaatg ggtcaaaaaa aagtttgaca aacgactggc tgtgtttgaa
   107761 tgccgaggat tgcctactga tgctaatagt tatgtacgga tgatctcagg tagttttatg
   107821 gtagctgata ttgataaatt taaagagata aaggggtttg atgagcgttt cttcatgtac
   107881 tttgaagaca acgacttgtg tttgagtttt gaaaaagcag gttacaagtt attgtacaca
   107941 cccttttatg cagttatcca tttttacggg aagggtgcgc atcgaaatgc aaaactgttt
   108001 cgcgtgtttt tgacttctat gatgaaattt ttcaataagt gggggtggcg ttttttctga
   108061 tgaaaccaga aatattagta gtcgttgtgc tttatcagca actattcagt caatctccag
   108121 cttatggacc attaagcaaa gcacttagcg aaaagcaaat tcagcttatt atttacgata
   108181 atagtcctca aaggcagcaa aatgagctgt ttgaaaaaga aaatatcgtt tatcatcatg
   108241 atccgcaaaa tcctggttta gcggtggctt ataattttgc gttgtctcaa gcagatgaag
   108301 gaacacgttg tctcgtcact ctggatcagg atacgaggct agtcgacgat tattttgaac
   108361 tattgcgtaa agtaaccttt acagatgaat gtgttgctgc agttccgatg attttttctg
   108421 ggagccgaca agtttctccc ctatattcag atcgttatat caatcgtaca gctcaagcag
   108481 tagaagttga aggcccaaca gctgaacgaa tcatggcaat caattcaggt gttgcctggt
   108541 ctgtcaaatt tctaaaaaag attggcgggt ttaatcctgc tttttcttta gattttttgg
   108601 atcattggct gttttggaaa gtggatcagt tggaaaaaac tgtggagatc cttcctgtac
   108661 gaatggaaca tgatctttcg gttttagact atgaaaaagt ttcaagtaat cgataccgtt
   108721 ctattttaca agcagaaaat cgattttatc atgaatatga tcaaaggcat ttatccagtc
   108781 accgacgaca attattgtta agaacagcta aacagttctt gaccgtcaaa aaccggcata
   108841 tctggcggat gactttgcgc tcatatgtta agaattggaa ggtgtgaatt cgtgcgttcg
   108901 gtttgtatag ctacctttaa tggaggcaga tatttaagag agcagttaga tagtatttta
   108961 tctcagcttt cagcagaaga tgaagtagtc atttctgatg atgggtcagt agatgataca
   109021 tgggatatat taactgaata cgcagaaaaa gataagcgaa tccagctttt ttcggggcct
   109081 cgtcaaggat tgatcgctaa ttttggttat gcgatccagc agacgaaagg agatctaatc
   109141 tttttggcag accaagatga tgtttggttg gagacaaaag tgaaaaagat cactgactat
   109201 tttgagcaga atccggatca gctagtagta gtttctgatt taatcattgt ggacagcgag
   109261 ctgcaagaga tccatgattc gtatttttcg tttcgaaatt ccaaagaagg atggcttaat
   109321 aatgtgataa gaagtcacta tattggtgct ggtatggctt tccggtcttc tttaaaaccc
   109381 gatattttgc cgatcccgtc agaagtgccg atgcatgata tgtggattgg tttgctggga
   109441 ggaaagaaga ctgggttcct aagagaaccg ttaactctgt accgaagaca tgatttaaac
   109501 gcgagtgaga ttgaaacaca ttctaaatta cggcaaaaaa tttcttggcg tgttcatctg
   109561 attacggcgt tgatcaaacg aaaatggttt aaacgttgat aaaagatgtt taaatcctta
   109621 atatttccat gacaaatata acaattgatg agataataac aaagtgaaat ttgaaatcaa
   109681 aaaggagagc agatatccat gaagggaatc attcttgctg ggggcagcgg aacacgtctg
   109741 taccctttaa caaaggcaac atcgaaacaa ttgatgccga tttatgacaa accaatgatt
   109801 tattatccaa tgtctactct gatgttggcg ggaatcaaag aaattctgat tatttcaacg
   109861 ccacaagata caccacgttt caaggaatta tttggaaacg gaaatgattt aggactgcag
   109921 atcgaatatg cagtacaaga aagtccagat ggattggcac aagcgtttat cattggagaa
   109981 gaatttattg gagatgatag tgtctgcttg gtattagggg acaatattta ctatggtggc
   110041 ggtctgtcta aaatgttgca acgagcagct tcaaaagata caggcgcgac agtatttggc
   110101 tatcatgtca atgatccaga acgctttggt gtagtagaat ttgatgagaa tatgcaagct
   110161 ttgtcgattg aagaaaagcc tgctcagcca aaatcaaatt acgctgtaac tggcctttat
   110221 ttttatgaca atgaggtaat ttctatcgct aaaggtatca aaccttcaga acgtggagaa
   110281 ctagagatta cggatgtcaa taaagcatat ttggataaag gaaaactatc cgtagaactt
   110341 atgggacgcg gatttgcttg gcttgataca ggaacacatg aatcattgct tgaagcatct
   110401 acctttatcg aaacaattga aaaacgtcag aacttaaaag tggcttgttt ggaagaaatt
   110461 gcttaccgta tgggctacat cgataaggaa caattgctgg cccttgcaca gccattgaaa
   110521 aaaaatcaat acggtcaata tttattgaat ttggccgctg aataaggagt ccttacagat
   110581 gaaagtaaca gatacaaaat taacagatgt caaaatcgta gaaatggacg tctttggcga
   110641 tcatcgcggt ttcttcacag aaagttattc aaaagcaaaa tttgcagaac atggattgaa
   110701 atatgacttt gttcaagaca accattcact atcagctgaa gctggtgtcc ttcgggggct
   110761 tcatttccaa aaaggagaag ctgcacagac aaaattgatt cgggtagtca ctggagcagt
   110821 tttagatgta gtagtggata tccgcaaagg tagcccgact tatggtcaat gggacggatt
   110881 tatcttatct gaacataatc atcgacagtt gcttgtacct aaaggatatg cccatggatt
   110941 cgttactttg acaccaaatg tgaacttcct ttataagtgt gataattact ataatgcaga
   111001 agcagatggg ggcatcgcat ttgatgatcc agatttagcg attgattggc caattgatcc
   111061 atcaagagcg attatgtcgg aaaaagatca ccgtcatccg acattaaaag aatttgaagc
   111121 agaaaatcca tttgtttacg gagaaatcta agaattaagg agaaattttc ttatgaaaaa
   111181 catcattgtt accggaggtg ccggattcat cggctctaat ttcgttcatt acgtggtaaa
   111241 caatcatcca gaagtgcatg tcactgtttt ggataaactg acttatgcag gaaacaaaga
   111301 gaacttggcc ggacttccta gcgatcgcgt agaattggtc gtaggagatg ttgcagatgc
   111361 agaattagtg gaccgtctag taaaagaagc ggacgcagtt gttcattacg cggcagaatc
   111421 ccataacgat aattcattaa aagatccttt cccatttgta caaacgaata tcattggtac
   111481 gtatacactt ttagaagctt gccgcaagta tgatgtacgc tatcatcacg tatctactga
   111541 cgaggtttac ggcgatcttc cattacgtga agacctgcca ggacatgggg aaggcgaagg
   111601 agaaaaattc actgcggaaa caccatataa tccttcaagt ccatattctt ctacaaaagc
   111661 tggttcagat ctgttagtaa aagcatgggt gcgttctttt ggcttacgtg caaccatttc
   111721 aaactgttca aacaactatg gtccttatca gcatattgaa aaatttattc ctcgccaagt
   111781 aacgagtatc ttaagcggta ttcgtccaaa attatatggc gaaggaaaga atgttcgtga
   111841 ctggatccat acgaatgatc attcttcagc agtgtggctg attttgacga aaggcagaat
   111901 tggcgaaacc tatctgatcg gtgcagacgg tgaagaagat aataagaccg tgatggaatt
   111961 gatcttagaa atgatgggcc aaccaaaaga cgcatacgac catgtaaacg accgcgcagg
   112021 gcacgatttg cgttatgcaa tcgattcgac aaaattgcga gaagagctag gttggcagcc
   112081 tgagtttacg aacttccgtg atgggttggc tgacacgatc aaatggtacg aagaacatga
   112141 agactggtgg aaaaaagaaa aagaggccgt tgaagcagca tatgccaaaa atggacaata
   112201 atacagagga aatttcactg tattaatgga aaaacacatg tgaggcaggc gctcgcctgt
   112261 ctctttttta aaataaaaat caaggagcgg ataaccatat gtttttatta actggaggaa
   112321 atggacaatt aggtacagaa ttgcgccatt tattagatga aaaaaatctg ccatatgttt
   112381 caacagatgc aaaagacttg gatatcacag atgctgagaa aacaatggct tatattacag
   112441 aattaaaacc cgatgttatt ttccattgcg cagcttacac agcagtagat aaagcagaag
   112501 aagaagcaaa agaattagat gagaaaatca atgtcgatgg cacaagaaat gtagcacttg
   112561 cagcaaaagc tgtgaatgca caattcgtct atatcagtac agactatgtt ttcgatggaa
   112621 aaaacaaaaa tgtagaatac gaagtagacg accaaacgaa tccactaaat gaatatggtc
   112681 gtacgaaatt attaggcgaa aaagcagtag aagagatttt agaagactat tatatcatac
   112741 gaacttcttg ggttttcggg atctatggac acaatttcgt cttcacgatg caacgcctag
   112801 cggaaacccg cgacaaactg actgtagtaa atgaccagtt tgggcgacca acttggacac
   112861 gtactttggc agaatttatg gtatatgtca tcgaagaaaa agcaccattt ggtatttatc
   112921 atttgtcgaa cgaaaatagc tgttcttggt atgagttcgc aaaagaaata ttgaaagata
   112981 cagatgtaga agttgcacct gttacgtcag aagaatttcc gcaaaaagca acacgtccgc
   113041 agtattcagt catgagcctg aaaaagacag aagcgttagg ttttgtcata ccaacttggc
   113101 aagaagcact tgcgcaaatg ctggaagctg ccaaaaaaat gaataagtaa tttccttttt
   113161 atcaaataga atttggagca tgatatggaa aaacaaacaa gaagtgcacg acgaaaagaa
   113221 aagccaaaag cgaatcaggc agattcgaat ttggtgcttg acctgtttct aaaactccgc
   113281 tatcttattg gcgttttagt catcgtggtc atcgttactt ttaatttaaa cggaagttca
   113341 cttggtgttt gggataaata tgtgtcgcaa agagacgatg ggaaaaaaac tgatgtgatt
   113401 tttggacaga atcgggaagt gcggtcagac gaatggctgg tgcagacacc tttttacctc
   113461 tcacaagctg aagaggactt ttcgttggtg aacgaggatt attcattaaa cggacaaaac
   113521 atgatcattg cgtataattc tcctgtaaag gatattacgg ttatcggaaa accattcaac
   113581 tggggattct ttttcctcgg aagagatcga ggactttcct tttattgggc gatgaaatta
   113641 gtcgtcatgg tcttgcttgg attcgaagta ctgatgatat taacgaagag gaaaaaagct
   113701 ctatcgctaa tcggtgcatt tgcgctgaca ttttctccag cagtccagtg gtggttcatg
   113761 caacatgtgg gcgatttgat tttctttact ttgggactga tgattgcttt ttatcattat
   113821 ttctacaatc atgataaaaa atggctacga acgttgatga tgcttttggt ggttattttc
   113881 ggtattgggt ttattttagt gatttaccca gcccatcaag tgatgctggc gtacttgctg
   113941 attttttatt ttgttggcct gcttatttac tatagcaaga aagtttcatg ggatctgttg
   114001 gatgtagtgt tgattggagg ggcagtttta tttattggcg gagtcatggt tcatttctgg
   114061 ctaacgtcaa aagacgcatt gttagcttct ttgaatacct tatatccagg aaaacgggta
   114121 agtactggag gaaagtggac gattggcgaa tttttttctt tccttacaaa ttggaaaatt
   114181 ccttttgaag atatcaacta ttcgaataac tctgaagtgg ccctgttcta tcactttttt
   114241 ccaactgtat tcctagcaag tccatttgtg ttatttggca aaaaagatag cgaacaaaag
   114301 ttactgggac gtattttgat gattttttgt ctgttttcta tcttttggat taccgttggg
   114361 ttgccaaaat ggctagcaga aatcactctg ctgtcttatg ttcctccatt aagagccatc
   114421 cttactttta gttttgcggc ttgtttatta tcgatctggt ttatcgctta tatttggaga
   114481 gaagatgtta ttcctatttg gtataaattc attttgttgg cagtaaatgc atggtcttat
   114541 ttctatgcga ttacgcattc gaaaatggat aattattttt cggattttga aaccatcgca
   114601 gttgctgtgt tgggaacagt gattttagct tttgcattat ttggctggag acgcttgttt
   114661 tacctcgctt ttgtcgcctt agttatcgta tctggctttt tcgtcaatcc agtcgtagaa
   114721 ggtacaggag cgatttttga aaaaacactg gcaaaagaaa tccaaaaaat cgatcaacaa
   114781 gatcctggta aagtttggtt gtctgaagat gaattatata acttcacccc aacattggga
   114841 gtgaaatcct tcaatacagt acgcttttat ccagatatag ctgcatggaa gaaaatcgat
   114901 ccgaaagaag aatacgagaa gtattataat cggtatgctc atacaagagc ttttatcgct
   114961 aaggaagata caaagttcat tttagatcgt ccagatatgt tttcggtcac gttgaatttt
   115021 gaagacgcag aaaaattagg gatcgactat gtgatcagta aacgaccatt aactgattac
   115081 aaccaattat atgaagcgca gttcacgcag ctatatggac cggataaaga cggttatatg
   115141 atttttaaag tagaatatcc ggattatcca gatgtaccac aaacacaaac tcaggaaaat
   115201 cagctagtcg tacagtaata ggctagctga gaataatcct gaaatattag tagaaaggag
   115261 agtgaagata attcaagcaa tcgtacaaaa atttgtcaac aaaatgagtg tcggtttctt
   115321 gcttttgttg ctgacgctag tcttttttgt ttggacagca ggggtaggtt ttccagcaac
   115381 tttgtttata tataacaata gttcattact tatcgctttt agtgtggcta ttttgctcat
   115441 tttaaatgtt tataaagcaa aaggcgtcga ttttctttat ctaggtgttg cgcttgccct
   115501 tttcattttc ttttcttact ttcgggaatt aagaaatgag tctttatttg gagacttact
   115561 gtttcctttg gttattttat tttgtttagt aattaaatgg atcgagtgga caaaatggga
   115621 tcgcggtatt tatctgattg tctttacggt cttcctcgga atcacgttat atcgtgtatt
   115681 tacagagatc caagtaccag aaggacaaag catctgggct ccaggcaatt cgctttctga
   115741 tatttggatc aatacgaata caatcggaag ttctttgatg actcttgctt tattgatcag
   115801 tggatttgcc agttcttttg aacgatggta tgttcgaatc atcggtggga tagcagtcgt
   115861 tgcagccttt ttaggtattt gggtctgcca atctagaggt gcattagtcg cgttgattgt
   115921 ttttgcgata ttggatcttt tacctaaatc gttgatgcgt gtcattcgtg cgccatttat
   115981 tgcctacacg gttacgattc ttttagcttt gccgatttct tatctggcgg ctgtctctga
   116041 aaaagtcaat ttgtttaccg atcgagaaga tatttggcat aaattctatc agacgctagg
   116101 agaaaaatct gaacaagttt tactaggaat gaaaacattt atcttccagc gaggaaacca
   116161 atttttagga aaccacaata gttataattc tattttgaat atctatggat tgataggatt
   116221 tgggattgct gctttgctgc ttatcctttt tattggtcgc ttaacgttga aagcagattt
   116281 atccaacggg cagatgactt ttatctgggc cttttttgcg gtgatgatgc agtcatttat
   116341 ggaggataca ttgacttcat acccatgggt tccgattatt tatatcctgt tggctatggc
   116401 cgttcatcga tatgatgaac cagaagttgc ggaggaattc tatgttatgg aagaggcata
   116461 tgacgatatg acttatgatg atacatctta tgaggaacaa ccagatagcg ctcattcttc
   116521 caggatccaa cgctatcatt gataggcaca aaatacgaga gaggctgtga caaccgtcgc
   116581 agcctttttt ggtttattgc aattgtctag catcttctgt aaaatagaaa cgttagagaa
   116641 aatcttgtat catagaaaat taagaaagaa ataagaacgc agctcggtac caaaaatatg
   116701 gtaaaatggc acttgactgt aattcactga aaatcgcttg gaggaacaca aatgtttaaa
   116761 gatttgtatt tatttatgaa ggacatttat cagaacagaa aattattgct acagttttcg
   116821 atcaatgact tcaaatcaag atatgcagga tcgtttttag gaatcttatg ggcgtttatc
   116881 aatccgctat ttactgtatt gatttattgg ctggtatttg gtgttggact gaaagcacaa
   116941 atgatcgatg caaaataccc atttatcgta tatttagtta ccgggatgat tccctggttc
   117001 ttcttctcag atgcatttgc atcaactacg gttgtttttc gggaatatac ttatttagta
   117061 aagaaagtcg tattcaatat tcgtatcttg cccacgacga aaatcctatc caatctgtac
   117121 acccatttat tcttcattct gattggcata gctgtcgcgc ttataaatgg tatatatcct
   117181 tcagcaatgt ctttacagct gatttattat ttattttgca tgtgtgcttt cttgacagga
   117241 ttgacttggc tgacggcatc gatccaacca tttttacctg atatcatgca attaattacg
   117301 atcgtgctgc agttgatcat gtggacatta ccgatattat ggagccctaa tcagtttgat
   117361 tcaggtcttg taagcatatt gaaattaaat ccgctgtact atgttgttca aggttatcgt
   117421 gagtcatttt taagtgaagc ttggttctgg gagcatggaa catatacttt gtatttctgg
   117481 gttttcacgt tagtaatgtt cgtaatcggc tctgcagtgt tccgtagatt gaaaccacat
   117541 ttttcagatg tgctgtaagg agaatagcta tgacagaata tgcaattaga ctaaaagata
   117601 tcacaaaatc ttataatatg tacgcaaaac cttcggatcg atttcgtgaa gccctcaatc
   117661 cttttaagaa atcctatcat gatgtatttt atgcgttgaa aaacgtgaat gtagaaataa
   117721 aaaaaggtga gatgatcggt ttcgtgggag aaaacggatc gggaaaatct acaatgttga
   117781 aaatcattac gggagtactg acaccaacat ccggcacgat ggaaatcgaa ggaaaaatct
   117841 cagctttgct agaactagga tcaggattca atcctgagta ttctggctat gaaaatatct
   117901 atttaaatgg aatggttcta ggattttcaa gagaagaagt cgatgaaatg gtcgatgata
   117961 tcattgaatt tgctgacatc ggagaccata tttatcaacc tgtaaaaact tattcaagcg
   118021 ggatgttcgt tcgtctagct tttgctgtag caatcaatgt gaatcccgat attttgattg
   118081 tggatgaagc attggcagta ggcgatcttg aatttcaact aaaatgtatg gaaaaattta
   118141 cagaaatcaa gaactcggga aagacgattt tattcgtttc tcatgatatc agttcgattc
   118201 gtcgtttttg tgatcgttcc tattggttga aaaacggaga agtcgtcgaa tatggcgata
   118261 cgatggaagt aacgacgaat tatgaaaact ttttaaagaa aaaatcagtt aaaacagtgg
   118321 atcggaataa aaaccaagta gaacggttcg ccgctccaga tatcgtcgac gtgacgaaag
   118381 cggaattgtt ggataaagac aaaaatccgg tggatatgct ggaacaagga gaaaaactat
   118441 acgttaaagt aacttatgaa gtaaaagatg atacaatcaa aaatccggtt ttaggaattg
   118501 cgctacgaac agtcgacaat ttttatgtat gcggacttaa tactctacta gatggtatca
   118561 agattccttg gaaaaaagga gagaatatct tttatctgga gtatgaaaag ttaggtttgt
   118621 tagctggtga atattatttt gacgttgcgg tctttgaaga aaatgcaacg gttcctttgg
   118681 tatataagac aaagtacatg aacttgtttg tcagcggttc ttatatcgga gaaggcatcg
   118741 tggtcttaga ccataaatgg gaagaaggga ctcatagcaa tgaaatatga ttttgaaatg
   118801 gacctagatg aacaaagcag tgtgggaaaa attgccgcac agatcaaacc aggaagtaaa
   118861 gttttagagt ttggtcccgg aaacggccga ttaacgaaac atttaatcgg agcaaaacaa
   118921 tgtgaagtga gcattgttga gttagataag gaattgttcg attttgtcag cgaatttgct
   118981 caagatggat tttatggtga tatcgaaagt tttgagtggg caaactatta tgctggacag
   119041 acatttgatt acgtcttgtt tgcggatgtt ttagaacatt tggtagatcc tggaaaaaca
   119101 ttgaagaaag tcagagaatt tttgaatgaa gaaggagaga tcctgattac attccctaac
   119161 ttggcgcata attctgtcat gatcgatttg ttcaataacc aattgccgtg ggcttcttat
   119221 ggtctcctag atgaaaccca taattctttt tatactcatg atggcttcca aaaagttttt
   119281 gaaaaagcgg gattattcat taatattgaa gattatttgt acttagctgt aggtgataca
   119341 gaattaaaat caacttatga agaattgccg gaagctgtac gctatgattt caaaatgcga
   119401 ccatttggtg aagtctacca atactttttc tcattgatga aacatccagt agcacaaagt
   119461 tccattgcag agccacaaaa ttctaattat gtaaaagtgt tagaagtaac ccagcaaacg
   119521 aaacaagatg aaacaatcca acaaatacca ttcaataatt tcacagggga aaatgagaca
   119581 ctaagtttcc ccgtatcaga aacaacagaa agaatggtgt ttcgcttcgc gcaacaacct
   119641 agctttatcg aattcagcgc agaagctgcc ggagaaaaac taacatttat cgattcgaat
   119701 gctgtagtca aaacagtaaa tgattgttat ttgtttgatg ggaaagaatt accagagttc
   119761 gttctaacgg atatttctgg gaaagaagtg actatccatt gtcactatcg ctttatcggg
   119821 gaattgacac caacaatgaa agaattgttg gaaacgattc gtcccatggc tgaagtcaca
   119881 aagcagcttt ctgagaaaaa tgacgaatta gaaagagaaa atcatcatct tcttgaggaa
   119941 aatacaagac tagaccatac gttaaaaaca accacaaatc gctattgcac attgttggaa
   120001 agtgatgaat ggacaataaa acatagacta ggacgacgta agaaagaaac ttcgaaaaaa
   120061 atccaagaaa aagaactttc tatatgtatt gacgagaaaa tctgggatgc agaaacaaaa
   120121 atattaaaaa tcatcggatg gggaatcgct aattcggatc gccagccctt gtcctataag
   120181 ttatcagctg accaatcgcc atttttcgaa gcaattcctg tttcacgaga agaagtcaat
   120241 caagcagaac agctcgcaaa aggaacagaa gcgggatttg agttacgaat cctttgcgag
   120301 caagaacgtt cattcttagt ggaagcagta gctcaaaatg gccaatcatg gatcattgaa
   120361 atgtaaataa tgattaactt ataaagataa ggagtaaacg aatcgtgcaa aaaaaagaaa
   120421 ttgccgtatt tatcgatcaa atcactcgtg aacgggccac tggcgatttg cttattgtgg
   120481 gctgggcaat tgatgaagtg accaaagaaa tccctacgat aaaggtagaa aaagaaaatg
   120541 tgattgctga agcaacacat gtcgtaagat tggatatcaa tcatttatat aatcttgatg
   120601 ttaagaccca aagcggtttc aaggtacgtt tatcagggaa aatgagagga aaagccatct
   120661 tagacttcca aacagcgaga catcaaaatg gcatagctgt gaaattaaac ggtaaatacc
   120721 cttatgatga cggcattgaa tcttcatggg aaagaaaaaa aagattactg aaaaaaggga
   120781 taaattatgc aaggactcat ggcgtaaaaa aagcgatccg tcgggtcaaa ttggaattga
   120841 agccaggatc gatcgactat gcagagtgga tcagtcgtca tgagaaacca gatttgaaag
   120901 agcaaaggaa agaagcacaa ggatttgcgt accgtccatt gatttccata cttatgcctg
   120961 tctataacgt tgaaatcaaa tggctcgaaa aatgcattga ttcggtactg gatcaaacgt
   121021 atgatcattg ggaactatgt atcagtgatg atgcttctac tgatccagct atccgaaagt
   121081 gtctggaatc ctatcaagca aaagatgata gaatcaaagt cgttttccga aaagaaaatg
   121141 gacacatcag tctggcaact aattctgcat tggaaatagc agaaggagaa tttatagcac
   121201 tcttagataa tgatgatgaa ttaccgccat ttgcattata tgaagtggca aaagtgctaa
   121261 acgtacatcc agaactggat gtgatctaca gtgacgaaga taaaatcgat gcagatggca
   121321 atcgctttga tccgcatttc aaagcagact ggtcgcctga tacattgatg ggaaataatt
   121381 atatctctca cttaggcgta tatcgaacaa gcattgttaa agaacttggc ggtttccgta
   121441 aaggctatga aggttctcaa gattatgatt tggtccttcg tgtgacagaa caaatccctg
   121501 cagatcatat ttaccacatt gaccgagtct tgtatcattg gcggacgatc ccaggatcaa
   121561 ctgcaagtaa tggcgaagcc aaaagttata tctatgattc cggagtgaaa gcattgacag
   121621 atgccctgtc tagaagaaac atcaaaggaa gcgtacgtcc tggcagaatt tctggatttt
   121681 atgagattgc ttatgacgtc ttgcaagagg atttggtcag tgtgatcatt ccaacgaaga
   121741 atggctatga agatctaaaa acttgtgtgg attcgatcat cgaaaaaacg tcttattcaa
   121801 attatgaaat cattattgcg gataacggaa gtacagatcc aaaaatgcaa gaattatttg
   121861 cagaatacaa gcatcagctg aaagaccgct ttatcgtaga attgattgac attccattca
   121921 attattctcg aattaacaat ttagcagcag aaaaagcaaa cggcaaatac tttttattct
   121981 tgaataacga tacagaagtg attgagccag attggatgac ggtgatggtt tcttatgcac
   122041 agtttgatcg aatcggctgt gtaggagcaa aactgtttta tccagatgat acaacgcagc
   122101 atgcaggagt attattagga atcggtggtg ttgcagggca tgcattgaat aactatgatc
   122161 gaacacattg tggttatttc ggccgattgg tcattgacgt caattactta gcagtcactg
   122221 cagcttgtat gatggtcaag gcagctgact tcaatgctgt taacgggttt gatgaaacat
   122281 tagaagtagc attcaacgat gttgacttgt gcttgaaagt ttatgaactg ggtcgttaca
   122341 atgtctatgc acatcaagca gaattgtacc atttcgaatc taaatcaaga ggttatgaag
   122401 atactccaga aaaacaaaga cgttttgctg gtgaaatcaa aaaaatgcaa gataaatggc
   122461 cagcatatat tgcgcatgat ccattttaca atgataattt aacaaaagac ggaatcggcg
   122521 acttttcgtt gagaccagat taaactataa taaaaagctg ttatgttgtg attcgctcgc
   122581 acataacggc tttttatagt tttttgtcaa aaaaaggcag aaggtgccga ctcaaaaaaa
   122641 taagtcgagg ttgggacaga agtgtttaac tccgagaaat aagaagaaat tcacgaaaat
   122701 tgcttttcaa atttttgtga atttcggctt atttaagaag aagttgcttc tgctcccacc
   122761 gtttattcgt ttttaaagaa tgagacaaaa gtgtttctta cttttgtccc acgctcttat
   122821 tcatgctcag agcgccaata gatacaaaat catagccaaa gtttcacgaa ccctagctgt
   122881 caaagaatcg atattttatt ttaatatagg ctattttaaa ataaatggtt taaggtattc
   122941 ctctacaaaa gccactctat caattatcat gggtttaaaa taagaagtga tggagataac
   123001 cagattgttc ttagcgtcaa tataaagaac atttccacta ttaccaattg ctgaatatat
   123061 ttcgttctca gaatctaata cccaccataa atagccatat ctcatatttt gtaagtcttc
   123121 taaagcactg gttctaggtt tgaacatctc gtttatccac atgggtggta cgatttgttt
   123181 tcctttatac atccctttat ttaggcataa tacaccaatt tttgccagat cacttgccga
   123241 taaacacaaa ccatatcctg ccgtcccgat atttagtgga tcacaaaacc agacattggt
   123301 tttaggagac ttatccattg taaactgctt atgttcttca gctgtttcag cataataatt
   123361 gacatggttt ggtatcccta taggatcaaa tagatactta ttggcaaaat caacagttgt
   123421 tagcttactt gtctgataca acaagccagt taatatgtgt aaacagacag tttgatagtt
   123481 gaattcgccg gttatacctt ttcttcctcc caataaatct agtgatgttt ttgtccaatc
   123541 ttcacttgaa cagacttttg tccatggatc gcctttacat ttgtagggtg ctttcattgt
   123601 tagcaagtgc ttgattgtaa tagcgtaaat agttttctcc ccgcgtttca ctttatactc
   123661 tggaaaataa tctaatactt tatcatcgat acttttgatt aatccttgct ctaaagcaat
   123721 gccaatcagc aaagatacaa tacttttcgt tgctgacatg atatgacaag tatcgtcttt
   123781 tttataacca ttccattcat ctgtataaac ttctacccca tccttatatg cgacaatttg
   123841 acatatattt ggttgtcgaa aaccaataaa atcatgcaac tcttgtttat tcataagata
   123901 ttacctcact ttcttctata agcataatgt cattaaaaat cgatttcaat tttttcgatt
   123961 cttcacgtaa tacaggtatt cttatggact agacagcgct ttgggaagat ggattctctg
   124021 aaaaatataa aggttcactt tatgagtgga taaaaaaagc agcaaatact tcgacttctt
   124081 taatttacca acattagtga aaatcgattc atctggagat ttgtaattta gagtccttgt
   124141 cttgattaga ggtgtataaa gctttttatt agacattttt aaattccatt acaatttgct
   124201 tataaactag gtttttaaag gtgactatgg taagataata gagatattat tttgtgatta
   124261 ggagtgtata tatgaataaa aatggagaat ggagtgctgc acgacggata ttcattatat
   124321 taatggatgt tattgtatac aatgcagcaa tatatagtag tttcttactg aagtttcgtg
   124381 gagagatccc attacgaaat ttagaaactt tccaacactc agcaattatt atttcagtga
   124441 tttttattgc gttgaacatt ttgttgggtg catatgtttt ttataatcga atgatcagtg
   124501 acatcgtgtt tgtcacagtc atcggtcagg tattgatgac actagggatc atggtagtga
   124561 cttttgcggg ccgctggttt gcttttccac gtcttgttat cttactttca tttgttctcg
   124621 gtacgctttt cttaagtatc tatcgtattc tcgtatataa actttattta cgcgtaagtc
   124681 gagataaaaa ggtcgtcatt attgggttag aaaaggatgt cgcaccagca atccaaaatt
   124741 ttggttcaaa gaaaaacaac aaacataaag tcgaagcagt cattcttgat aactattatg
   124801 caaacatcaa aaatatgatt aaagacatcg atattgtcta tcttgcttct catatcgaag
   124861 aaactgaaaa aatcaaaatc tatcaattag ttactaaaaa tgaaaaaaaa ttgtttttga
   124921 atacaacatt tgaaaacttg acattgatca atccaaatat tatgaatttc gaagatgaaa
   124981 gtattattga agcatctgga tttagaatcc aacctgaata tgaactattc aaacgtttgt
   125041 ttgatatcat cgtttcactt gttttattag ttattgcttc accatttatg ttattcacag
   125101 caattttagt caaagtgaca tcaccgggac caatcatcta taaacaagtg cggatcacaa
   125161 aaaatcaaaa ggaattttca atctataaat tccgctcgat gaccgccaca gccgaagcaa
   125221 aatctggacc agttttagct aagagcaatg atgcacgagt aacgcctgta ggtaaattta
   125281 ttcgagctgt ccgctttgat gaattaccac aaatcttcaa tgtgctgaaa ggcgatatgt
   125341 cgatcgtagg accacgtcct gaacgttctt tcttcgtcga tcagttcaat gcagaaaacc
   125401 cttattacta cttacggcat aatgttcgtg ctggtatcac tgggtatgca caagtctatg
   125461 gaaaatatgt ctctgattac aacagtaaat tacgttttga tttactttat atcaaaaaat
   125521 attcattgat gcttgattta aagatcttgc tgcaaaccat caagatccta tttgataaaa
   125581 tgtcctctca aggacttgaa gaagacgaaa atgtgatctc attggcagaa attgcttttc
   125641 cgaatgaaaa aatctttcgc tagttgggag gcgtaaaaat gaacaatccg aaattcagca
   125701 taatcgtacc agtatacaat gcagaagcaa ctattggtcg aacgattgat attctaaaag
   125761 aattggatta tgaagagtac gaagtcgttt tagtaaatga tggatcaaca gatcaaacac
   125821 aggaaatcat tcaagaactg attgaaaatg atgaacgttt tgaattgatt actacgacca
   125881 atcaagggcc cggattagca agaaacgaag gaataagaca gtcaaaagga gaatatctct
   125941 tattcttcga tgcagatgac acgccagtaa aaacgatctt gtcagattat gatcatttgc
   126001 tagaaaagag tccaaaagcg gatcttgttg ttagttcgtt tgtttttcgt acaatggacg
   126061 agggaagaat cgtttccgaa aaagaaaact tagttacaga atgtcactat ctaactcacg
   126121 aagagtttat aaaagacatg tatcagttaa tgaatgaaca gttgatgtat gttgtatgga
   126181 acaaatgcta tcgtagcgat attctgaaaa ataaccaaat tctatttaaa acttataata
   126241 gttgtgagga tcgaatattt aacttacatt attatgaata ctgtcatcaa gtgatcatga
   126301 acccaaaagt agaatatatt tatgaatttg aaggtgggaa ggggatcact aatcaatacc
   126361 gacctaacaa gttcgaaaca tttaaagaat tctattcatt agcaaatact gtaacagacg
   126421 atattaataa agacggtatg gcggcgcttc ttttaaaagg gacaacttca gttatttttt
   126481 ctatttatag tacctctgca agaacttcaa aagagaagaa agacgaagca aagcagatat
   126541 tgagtgatcc agtgatccga gaagccaagc aaattgctaa gacggattct tcagcaaaaa
   126601 aaataacaaa gttgctttac aatatgccag ctccactatt tttatctgct gtaaaagttg
   126661 gaagcttcgt tgaagtgaaa atgccagggt taatggctgc tttgaagcga gtatattagt
   126721 taatggagga aaatataatg aaaagaattt tgcattttca gggacgaatg ggtttaggtg
   126781 gagcagagtc tttcatgatg aatttatatc gtaaaattga tcgtacaaac tatcaatttg
   126841 attttttgat ttatgaagat tatgcagatg ttcaagacta tcattcagag attgaaagat
   126901 taggaggccg tatttttgtt gtacctaatc ctaagaaaaa tatttttaaa tatttaatag
   126961 aagtgaataa gttactaaaa aaagaatctt tttctatagt tcacaatcag gtttattttg
   127021 gcgggggaat taatttatgg ttagccaaaa agaatggaat cagacagaga attgctcata
   127081 gccatgcaac tgaagatgga aaatctcaaa atattgtaat gaatgtattg cgtaaatttt
   127141 taaccaaatt gttgttacaa aatgcaacag actaccttgc cgttagtcaa gaagctggtg
   127201 aaagtctgtt tcaaaaccat ccctttgaga tagttcataa tggtattgat ttagagcttt
   127261 atagtaaaaa ttcagaagct aaggttaata agagaaaaga attggatatc tcaatgagta
   127321 cttttgttgt gggaaacatt ggacgattag agaaacaaaa aaatcaatct tatttgttag
   127381 agattttttt agaaatacta aaaatgaatt ctgattctta cttgatttta gtaggtgaag
   127441 ggtcacttag aaataaactg aaagccaaag cagtagaatt ggatatagag aaaagagtaa
   127501 aatttttagg agaaagaaac gatattcccg agttattatc tgtgatggat gtttttgtaa
   127561 tgccttctct ttatgaaggt ctgccaatgg ttggattaga agcacaagca gcaaaattaa
   127621 aattagtctt agcagatact atttccaaag atacaaagtt aacaaataat gtggattttg
   127681 tatcattaga tgaaacacca caagaatggg caaaaaaagt aataaaagag ccgtatgcta
   127741 ataaaataac acaagaacta aagagttatg atgtttcgta cactactgaa caaatggaga
   127801 gtatatatag tagagatcat taaaaatgtt caagatattt taggagtgat tatttttgaa
   127861 aatatgttat ttgatattgg catacaatga tattggcaac ttaaagagat tggtcaatcg
   127921 attgaacgaa aatgcagttt ttattattca tatagataaa aaaactgata tagaaccatt
   127981 tattgaagca tttaaatcat atgataatgt tgaattttta tgtaataaaa accgtgttaa
   128041 aatttattgg ggtggttttt ccattgtaca agcagaaatc aatttagtaa agagagcatt
   128101 acaaaatgag aaatatttaa aatatgtatt attatctggt gctgattatc caatcaaaga
   128161 taatgagtat atttataatt attttaaaaa gaatagttca gtggagttta ttcgtggaat
   128221 agatctagat caaattaaac ataaagaatt ttattataaa catatagatg tatatcaaaa
   128281 acatgattat cctagaatta ataggaataa cacaactgca tttaaaattt ttagagctat
   128341 aattaatcgt tgtctgagga tgattaaact tcccccaaaa attagacacc ataaatttga
   128401 tttgtatcat ggttctcaat ggtgggcttt gtcgaaagaa tgcctaacag aattaataca
   128461 aatgtatgaa caaaatcaac aagactacct gaattttaaa ataggtatgt ttgccccaga
   128521 tgaaaaattt tttcatacac ttttttttaa ttcctcgttc aaaaataaaa atgttattgg
   128581 aggaccagat ttaccacttg aattgaaaaa tattgaagaa actactttac aaacttcgaa
   128641 attagcaaat attcatatta ttgatccttc catgaacaaa tggtttgatg aaagggatat
   128701 agatcaaata aaagaaagtg aaaaattatt tgtaagaaaa gttaaatcgg attactctga
   128761 taaactatta aatagaattg ataaagaaat tttgaaagtt ggaaatatag atgaatagaa
   128821 tagtacctgt tttagagaag agtttattaa gtcgagatgc gtttcgagta ataaatttaa
   128881 tcatttgtct gctttatttt atgcctagta cttttaatgt aactaatatc ccgatgaaaa
   128941 tagcttttgc ttggggtgga attatagttt tgtatgaggt ttttataaag aaaaattttt
   129001 ttaaaatgcg tttttcttgg ctactgattg gagcaatgat tagttttatc ttaactataa
   129061 tcattaacag agaaaatttt ttaattccat ctatttataa ttttggatat ttgctgatta
   129121 ctttgatagt aatttttcct gcagactttt cacaaagtga agaggaaaaa aagaaaaaaa
   129181 tggtgtattt taataatgtt ttcattatca taattttttt agctgcgcta atatctatct
   129241 atcaatttat ttttttgatt tcttatcatg tcccaacagg aacgccagga ttacttgcaa
   129301 gacaaggttt tattgaacat cggttatttg gtgtctatac aagtccaaat gttggagcta
   129361 tgtttggata tacttctatt attttaagtt taattagtgt attaatttca aaaataacaa
   129421 aaacgattcg tgttttttat atgatgaatt ttatcgtaca aatattttat attacacttt
   129481 cttcgtctag aggtgcccag gtagtaattt gttctttttt agtattattt cttttgttgt
   129541 ttgccaattc aaaatttaga aagagcatta gagaagtact acctgtaaaa atcaaatgga
   129601 tggtaagcat attattagtt ctattggtat atttttcttt tggtactaat tatgctaagg
   129661 attttttagc aacaattcct gtatcgattg atagaaaagt agaagttata gagtcaaatg
   129721 aatcagataa aactccactt ccagaaaaag aagtaatatt acagcattct agtgataact
   129781 ccgaaatttc agctggtcgt tttacgattt ggaaagctgc gcttaaagtt ataagaccaa
   129841 ctttgttatt tggttatgga gagaccgatt tctatgggaa tagcgatgca aaatttttga
   129901 aaaaagtaga tcttgatact acagatgtga atgaactaaa aagagcacat ggaaacatgc
   129961 ataatggatt tcttcaagta cttgtatcaa gtggtataat tgcttttata tgtatctatg
   130021 ctttctatgt gttaaatatc tttagtttac tcaaaacatt ttttagaagt aaagtgaatt
   130081 atgcgtttct tggtattgtt ttaattttta tactatctat ttttatagat gaaatggtgg
   130141 aggctcatgt acttttcaat aagcgcgatg tcatttcaat tgttttctgg tattatttgg
   130201 gaagcatttc ttacttgtat ttaaaaaagg agaattaata tgaagacagc tttcattgct
   130261 tggactcctt tacatttgat taatatatta aatgtttatc aaaattttta ccgagaatca
   130321 aaaggagatt tatttattta ttctgaattt gaaaacgcta gaagatatag tgagcgtata
   130381 aaagaattag ggctatttaa tgatgttgtt tttgtgaatc accggaaaat cggttcttcc
   130441 tttaaacgta aaattaactt gttaactaac cataatcaat ttttctcgga tgatttatcc
   130501 gtttatgatg aaatttttat acagggggaa aattatttca caaaaatact tttctccaat
   130561 gtttacaaaa aa
//
LOCUS       DAJSBL010000003        87928 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.003, whole genome shotgun sequence.
ACCESSION   DAJSBL010000003 DAJSBL010000000
VERSION     DAJSBL010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 87928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 87928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..87928
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.003"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(553..2166)
                     /locus_tag="ONT85_000269"
     CDS             complement(553..2166)
                     /locus_tag="ONT85_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCR2377272.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
     gene            complement(2307..3215)
                     /locus_tag="ONT85_000270"
     CDS             complement(2307..3215)
                     /locus_tag="ONT85_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR2377273.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYTGLSPSKYYLSLK"
     gene            3398..4522
                     /locus_tag="ONT85_000271"
     CDS             3398..4522
                     /locus_tag="ONT85_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HCR2377274.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
     gene            4749..5666
                     /locus_tag="ONT85_000272"
     CDS             4749..5666
                     /locus_tag="ONT85_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCR2377275.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            5659..6492
                     /locus_tag="ONT85_000273"
     CDS             5659..6492
                     /locus_tag="ONT85_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCR2377276.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVLATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            6575..7693
                     /locus_tag="ONT85_000274"
     CDS             6575..7693
                     /locus_tag="ONT85_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HCR2377277.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            7687..9585
                     /locus_tag="ONT85_000275"
     CDS             7687..9585
                     /locus_tag="ONT85_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HCR2377278.1"
                     /translation="MVVKRGENTMKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQ
                     IDYIHQGVGYGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYW
                     GEIHERDQRMVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQISEYECADGNWQF
                     FKIIVGTIIQKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYG
                     LIYSVLEPEKPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAML
                     WADLLPFKLGEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLW
                     LNKIFLLLALPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPN
                     FHTLTNEKYLKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKT
                     YGQFIVRNNVRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLY
                     ETGFAIECSPEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLM
                     TWENTFIPGLEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKN
                     NKKRLKLC"
     gene            9579..10802
                     /locus_tag="ONT85_000276"
     CDS             9579..10802
                     /locus_tag="ONT85_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HCR2377279.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(10888..12822)
                     /locus_tag="ONT85_000277"
     CDS             complement(10888..12822)
                     /locus_tag="ONT85_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HCR2377280.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            13048..13704
                     /locus_tag="ONT85_000278"
     CDS             13048..13704
                     /locus_tag="ONT85_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HCR2377281.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            complement(13778..14131)
                     /locus_tag="ONT85_000279"
     CDS             complement(13778..14131)
                     /locus_tag="ONT85_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HCR2377282.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            14323..15252
                     /locus_tag="ONT85_000280"
     CDS             14323..15252
                     /locus_tag="ONT85_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HCR2377283.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            15263..16099
                     /locus_tag="ONT85_000281"
     CDS             15263..16099
                     /locus_tag="ONT85_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HCR2377284.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            16347..17123
                     /locus_tag="ONT85_000282"
     CDS             16347..17123
                     /locus_tag="ONT85_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR2377285.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            17299..18042
                     /locus_tag="ONT85_000283"
     CDS             17299..18042
                     /locus_tag="ONT85_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR2377286.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            18035..19615
                     /locus_tag="ONT85_000284"
     CDS             18035..19615
                     /locus_tag="ONT85_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HCR2377287.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(19866..20345)
                     /locus_tag="ONT85_000285"
     CDS             complement(19866..20345)
                     /locus_tag="ONT85_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HCR2377288.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(20361..21113)
                     /locus_tag="ONT85_000286"
     CDS             complement(20361..21113)
                     /locus_tag="ONT85_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HCR2377289.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            21230..21300
                     /locus_tag="ONT85_000287"
     tRNA            21230..21300
                     /locus_tag="ONT85_000287"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21262..21264,aa:Gly,seq:ccc)
     gene            21661..22386
                     /locus_tag="ONT85_000288"
     CDS             21661..22386
                     /locus_tag="ONT85_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HCR2377290.1"
                     /translation="MSKKTTKAIVGIVAAVIAGAFGVSTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            22641..22907
                     /locus_tag="ONT85_000289"
     CDS             22641..22907
                     /locus_tag="ONT85_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HCR2377291.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            22904..23335
                     /gene="ruvX"
                     /locus_tag="ONT85_000290"
     CDS             22904..23335
                     /gene="ruvX"
                     /locus_tag="ONT85_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HCR2377292.1"
                     /translation="MRVMGLDVGSKTVGVAISDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            23360..23665
                     /locus_tag="ONT85_000291"
     CDS             23360..23665
                     /locus_tag="ONT85_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HCR2377293.1"
                     /translation="MTEHNHSHDHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEEE"
     gene            24234..25071
                     /locus_tag="ONT85_000292"
                     /pseudo
     CDS             24234..25071
                     /locus_tag="ONT85_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325086.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
     gene            25136..25570
                     /locus_tag="ONT85_000293"
     CDS             25136..25570
                     /locus_tag="ONT85_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR2377294.1"
                     /translation="MLREMRLEDAEALREINADQLGYDIPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKYAEKRGIGKALMTGVEEE
                     AKKRGITTIRLNSAEYRVEAHQFYEHIGYHSDKMQKRFLKIL"
     gene            complement(25638..26960)
                     /locus_tag="ONT85_000294"
     CDS             complement(25638..26960)
                     /locus_tag="ONT85_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HCR2377295.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFWDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRTVKPSIPQLGVGLMVSL
                     TFFILGQIGNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(27114..28754)
                     /locus_tag="ONT85_000295"
     CDS             complement(27114..28754)
                     /locus_tag="ONT85_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HCR2377296.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAAFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAAAILEKYEDQITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQIILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     EGLTPGQIPFARKRSDFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQNLNREEITDIKEAVESAKWVPEYKSLED"
     gene            29084..29554
                     /locus_tag="ONT85_000296"
     CDS             29084..29554
                     /locus_tag="ONT85_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HCR2377297.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLDSNQAIISELTV
                     GGLVDSLALAEGFTGEDIYEMFSEVRQLKKGEKCIEVHHESPNVRFKRGDNEMIFKNH
                     VSDHTELYEIVNQILSGIFELGPYEFKEENGNPKLNEEWQETIETQRNNPHLFQ"
     gene            30183..31286
                     /locus_tag="ONT85_000297"
     CDS             30183..31286
                     /locus_tag="ONT85_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HCR2377298.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHRLIIPHVLRDVELPDTTTHFDEEMLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDNGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
     gene            31570..32250
                     /locus_tag="ONT85_000298"
     CDS             31570..32250
                     /locus_tag="ONT85_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HCR2377299.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGSLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            32340..33374
                     /locus_tag="ONT85_000299"
     CDS             32340..33374
                     /locus_tag="ONT85_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HCR2377300.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTIPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNEFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            33388..33723
                     /locus_tag="ONT85_000300"
     CDS             33388..33723
                     /locus_tag="ONT85_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2377301.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPVPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEEKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            33707..36187
                     /locus_tag="ONT85_000301"
     CDS             33707..36187
                     /locus_tag="ONT85_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2377302.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLADIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISTSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSVQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYEAKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            36243..37802
                     /locus_tag="ONT85_000302"
     CDS             36243..37802
                     /locus_tag="ONT85_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR2377303.1"
                     /translation="MRRKLLLLKSEMLGGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     ISVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQICYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFTITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDCPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            37799..39163
                     /locus_tag="ONT85_000303"
     CDS             37799..39163
                     /locus_tag="ONT85_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCR2377304.1"
                     /translation="MKIVIIGASHAGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQAIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEHTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            39334..40404
                     /locus_tag="ONT85_000304"
     CDS             39334..40404
                     /locus_tag="ONT85_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR2377305.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAVGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            40419..41087
                     /locus_tag="ONT85_000305"
     CDS             40419..41087
                     /locus_tag="ONT85_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR2377306.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEAKELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            41107..41685
                     /locus_tag="ONT85_000306"
     CDS             41107..41685
                     /locus_tag="ONT85_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCR2377307.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            42088..42999
                     /locus_tag="ONT85_000307"
     CDS             42088..42999
                     /locus_tag="ONT85_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HCR2377308.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(43255..44172)
                     /locus_tag="ONT85_000308"
     CDS             complement(43255..44172)
                     /locus_tag="ONT85_000308"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HCR2377309.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELHKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            44374..44811
                     /gene="zapA"
                     /locus_tag="ONT85_000309"
     CDS             44374..44811
                     /gene="zapA"
                     /locus_tag="ONT85_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HCR2377310.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            44869..45399
                     /locus_tag="ONT85_000310"
     CDS             44869..45399
                     /locus_tag="ONT85_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016630349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HCR2377311.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELW"
     gene            45531..47891
                     /locus_tag="ONT85_000311"
     CDS             45531..47891
                     /locus_tag="ONT85_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HCR2377312.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKLENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNAYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            47978..48292
                     /gene="trxA"
                     /locus_tag="ONT85_000312"
     CDS             47978..48292
                     /gene="trxA"
                     /locus_tag="ONT85_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HCR2377313.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            48474..50252
                     /gene="uvrC"
                     /locus_tag="ONT85_000313"
     CDS             48474..50252
                     /gene="uvrC"
                     /locus_tag="ONT85_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HCR2377314.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKKFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELANKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMAKNDKHKTNELLFGIDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            50397..51296
                     /locus_tag="ONT85_000314"
     CDS             50397..51296
                     /locus_tag="ONT85_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR2377315.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            51289..52551
                     /locus_tag="ONT85_000315"
     CDS             51289..52551
                     /locus_tag="ONT85_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR2377316.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPVRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            complement(52645..53937)
                     /locus_tag="ONT85_000316"
     CDS             complement(52645..53937)
                     /locus_tag="ONT85_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HCR2377317.1"
                     /translation="MSYTHLIKETLDILDLSVTFNENCLTKEKYKGQICHIYRGNLIY
                     TAQECIHCKHQIASDIVRWGTTTVRLLMNDVSECRTYLELKKQRFKCKACQRTFVADT
                     SVAKKHCFISEKVRWSVVTRLKKNTSMTEIAAQKNLSVSSVYRIMKRFYRPLNPFRTP
                     LPKVLCFDEFKSVRGVSGAMSFIMMDGQTQRLLDIVENRQLPFLKRYFSHFSREIREA
                     VEWIVIDMYAPYVSLVKKLFPKAQLIIDRFHIVQHIGRTFRNHRIKETNQLLKSKEQK
                     HYQLGKQLKRYWKLLQKDERKLDYTRRLWRPGFKAHLTETDIVDRLLKGSPALRVGYQ
                     LYQDFLYAVKERDYVSFEELLTNNIMLPEGYQTTLRTFQKFLPQIKNALQQSYSNGPL
                     ECLNNHIKVLKRNAYGFRSFYNFKLRIMIRHGNALIFN"
     gene            54221..56119
                     /locus_tag="ONT85_000317"
     CDS             54221..56119
                     /locus_tag="ONT85_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HCR2377318.1"
                     /translation="MDRYLDLLSEKFPTTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETEISQFTIFTAYPEYVLTTDWYKKQDKS
                     QLIHRLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFTP
                     KNRKDLSEREQEELEKIHQALSILQFKSEHQLLNRRPEFKMDDRKLLEKINYEESTIV
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTANEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDAKEEGSAIINGHTPVKVKKGESPIKANGKAFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRNTTKGMELQQQITQLQR
                     LLDYRKDD"
     gene            complement(56418..57137)
                     /locus_tag="ONT85_000318"
     CDS             complement(56418..57137)
                     /locus_tag="ONT85_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HCR2377319.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIEKIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFATHEVKIVSD
                     SLLSDLLPDSYFVNSYHHQAVKDLSPLLKAIAFSNDGLIEAVQSKDNMHKILGVQWHP
                     ELSHRVDSSEQRLFDFFVNEF"
     gene            57474..57806
                     /locus_tag="ONT85_000319"
     CDS             57474..57806
                     /locus_tag="ONT85_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2377320.1"
                     /translation="MKQVLEALEQIRKAEEENEKRARTLRQQLKTYAEEKEQELEIIQ
                     TNSRQQLKQLVEEKEQFEKQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            57793..59796
                     /locus_tag="ONT85_000320"
     CDS             57793..59796
                     /locus_tag="ONT85_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HCR2377321.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFMNKLEEVLDLKEKWEQLTEKRDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAAIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKVKERFIHATYLIVLQGWVDAEEKQNLMHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLIEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLFVLPRGMKRFVKFFEILSVPSIIWGLIYSSFFGQALPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALIGVMLIKNTAFVYLGAGLSIIAALCILIVPIIQSPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            59815..60285
                     /locus_tag="ONT85_000321"
     CDS             59815..60285
                     /locus_tag="ONT85_000321"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HCR2377322.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            60306..60890
                     /locus_tag="ONT85_000322"
     CDS             60306..60890
                     /locus_tag="ONT85_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HCR2377323.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQIETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAIAEMENWDESEQ
                     IQFIKNSLYSLPLTGKVTFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            60905..61891
                     /locus_tag="ONT85_000323"
     CDS             60905..61891
                     /locus_tag="ONT85_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HCR2377324.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNLDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFEPETIRKRVRQVYDA"
     gene            61881..62192
                     /locus_tag="ONT85_000324"
     CDS             61881..62192
                     /locus_tag="ONT85_000324"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HCR2377325.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            62214..63995
                     /locus_tag="ONT85_000325"
     CDS             62214..63995
                     /locus_tag="ONT85_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HCR2377326.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            63988..65364
                     /locus_tag="ONT85_000326"
     CDS             63988..65364
                     /locus_tag="ONT85_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HCR2377327.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            65371..66006
                     /locus_tag="ONT85_000327"
     CDS             65371..66006
                     /locus_tag="ONT85_000327"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HCR2377328.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            66036..67391
                     /locus_tag="ONT85_000328"
     CDS             66036..67391
                     /locus_tag="ONT85_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HCR2377329.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(67583..68977)
                     /locus_tag="ONT85_000329"
     CDS             complement(67583..68977)
                     /locus_tag="ONT85_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HCR2377330.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            69069..70040
                     /locus_tag="ONT85_000330"
     CDS             69069..70040
                     /locus_tag="ONT85_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HCR2377331.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            70037..70819
                     /locus_tag="ONT85_000331"
     CDS             70037..70819
                     /locus_tag="ONT85_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HCR2377332.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(70988..71359)
                     /locus_tag="ONT85_000332"
     CDS             complement(70988..71359)
                     /locus_tag="ONT85_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCR2377333.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            71493..71936
                     /locus_tag="ONT85_000333"
     CDS             71493..71936
                     /locus_tag="ONT85_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HCR2377334.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(71979..72788)
                     /gene="yidA"
                     /locus_tag="ONT85_000334"
     CDS             complement(71979..72788)
                     /gene="yidA"
                     /locus_tag="ONT85_000334"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HCR2377335.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(72807..74174)
                     /locus_tag="ONT85_000335"
     CDS             complement(72807..74174)
                     /locus_tag="ONT85_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HCR2377336.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(74158..74988)
                     /locus_tag="ONT85_000336"
     CDS             complement(74158..74988)
                     /locus_tag="ONT85_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HCR2377337.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLKDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            75151..75588
                     /locus_tag="ONT85_000337"
     CDS             75151..75588
                     /locus_tag="ONT85_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HCR2377338.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTGGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            75664..76284
                     /locus_tag="ONT85_000338"
     CDS             75664..76284
                     /locus_tag="ONT85_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HCR2377339.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            76473..76979
                     /locus_tag="ONT85_000339"
     CDS             76473..76979
                     /locus_tag="ONT85_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HCR2377340.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            76996..77895
                     /locus_tag="ONT85_000340"
     CDS             76996..77895
                     /locus_tag="ONT85_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HCR2377341.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            77982..78866
                     /locus_tag="ONT85_000341"
     CDS             77982..78866
                     /locus_tag="ONT85_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HCR2377342.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNYTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(79058..79129)
                     /locus_tag="ONT85_000342"
     tRNA            complement(79058..79129)
                     /locus_tag="ONT85_000342"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(79094..79096),aa:Arg,seq:ccg)
     gene            79189..80523
                     /gene="rpoN"
                     /locus_tag="ONT85_000343"
     CDS             79189..80523
                     /gene="rpoN"
                     /locus_tag="ONT85_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HCR2377343.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            80672..81763
                     /locus_tag="ONT85_000344"
     CDS             80672..81763
                     /locus_tag="ONT85_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="central glycolytic genes regulator"
                     /protein_id="HCR2377344.1"
                     /translation="MGRFKSTLDVKCPTKEIAMLDEIKMIESVAPDIIEVLQERYKIL
                     RNIYWMQPIGRRSLSESMGITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSL
                     ENFMDQLLGTHQTEQQLAEYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEG
                     ENIIAVMGGTTMAVVAEQLSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKAN
                     GNHRALYVPEQLSLATYNSLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEK
                     ELVMLKQANAVAESFGYFFNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAI
                     RAYMKNAPKQTWLITDEAAANEILKGVTL"
     gene            81804..82805
                     /gene="gap"
                     /locus_tag="ONT85_000345"
     CDS             81804..82805
                     /gene="gap"
                     /locus_tag="ONT85_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HCR2377345.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            82915..84105
                     /locus_tag="ONT85_000346"
     CDS             82915..84105
                     /locus_tag="ONT85_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HCR2377346.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            84209..84964
                     /locus_tag="ONT85_000347"
     CDS             84209..84964
                     /locus_tag="ONT85_000347"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HCR2377347.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            85080..86378
                     /gene="eno"
                     /locus_tag="ONT85_000348"
     CDS             85080..86378
                     /gene="eno"
                     /locus_tag="ONT85_000348"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HCR2377348.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
     gene            86777..87916
                     /locus_tag="ONT85_000349"
     CDS             86777..87916
                     /locus_tag="ONT85_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HCR2377349.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
BASE COUNT        28900 a        15226 c        18142 g        25660 t
ORIGIN      
        1 gttaaataaa taggaacaaa aaaattaaat tatttaatac acacattagg gatcattcat
       61 tgaaggatta gaagacaacg aggatggcga aaaatatgat tacaatagaa aagtcgttct
      121 aaaccaatca gttcctagta tcacacaacg caagccagtc gatttggtga gattagagga
      181 gtgaaagcaa actttattat ataagaaaaa atattcgaaa gggatgctag tttaggtact
      241 agtgctgata gattaatatg atgcctcgta atattaggcg tagttactct agagacatca
      301 ctatggacta tgatttgtct cagagtcaca acatgttttt ttatgaaaag aaacagctaa
      361 aataattaaa ttgcgcaatg tttaaacttt cttaaactat aacctaataa atgtgaagaa
      421 ataatgcaca atttcttcac atttatcttc tattctatat acataccaac caacaaccct
      481 atatactttt tcatttttac tcctcaagta aaaataactc ctagaccgcg gctacccacc
      541 gcggtcgttt ttttattctt caatcaaaat cgctttgata cccaaacctg ccaagattcg
      601 ggcagaagta taagtattca atcctcttct gaaagtaatg taaacttttt tatcttttgg
      661 gatttcattg attcgatctc gtaactcatt cattggaata ttttttgttg ccgagatact
      721 gccagaaggt gctttattag gttcacgtat atctaaaaag aacgcttctt tcaaatcgtt
      781 ttcgggtata tcacttgctt tgatcgtttc tacgatattc gtcatttgat tgattgcgac
      841 ataaccagct atattcaacg ggtcacgggt agtagaatac ggtggagagt aaggcaactc
      901 caaatcaggc aaatcaaaga ctgtcaagtt tccggtgatt gctacagaaa gttctcccat
      961 ccgtttatcg acaccttttt ctccaaatgc ttgtccacct aaaatacgac cgctatctgc
     1021 atcaaaaatc agctttagat ttaatcttgt agcttctgga tagaaatagg catggtcaaa
     1081 gggagtgata aaaacagttt tataatttgt tatgcctgat gcttttaatt catgttctgt
     1141 cattcccgca taacttgctg tatgatcaaa gatttttgcg acacctgcac caatatatcc
     1201 gcgatagcgc ataggcgtac catttaaaat atctgccagc atatgtcctt gacgattggc
     1261 cgcactggaa agcatacttt ggatcggctg accagtcact acactagtcg tttcgataat
     1321 atcgccaatc gcatagatgt ccggcagatt ggtttgtaac tggtcatcaa caatgatctg
     1381 acctgtatct gataattgta tgccggttgc tttcaccact tcattcttcg gagaaacacc
     1441 aacagcaaaa attagcatat cagcagaaag gacggagcca tctgataata ctatttctcg
     1501 acctttatca cggatctcag taactcttgt ttctaaatga acggctaatc cttctttcag
     1561 caatttgtca taaactaaat tagcaatttc tggatcataa gggaaagcga cttgtggcaa
     1621 ttgatcgatt aaagtcacgt ctaagccgcg atgcttgaaa ttctcagcta attccagacc
     1681 cataacacct gcaccaagaa tgacgacatg ttgtggattt ttttcttcaa ggaacgattt
     1741 gattcgatcc gcatccgtca cactacggag gacaaagccg ttttctgctt caaaaactcc
     1801 gggaatatcc ggataatctg gtctagcacc agaagagatg atcaacttat cgtaatctgt
     1861 ttttatttct tcatttgtgg ataggtcttt aactgttaat cgcttagtag aaggatcgat
     1921 tgctgttact tcgtgtttag taaagacatc gatgttgttt tttgttttca atatttctgg
     1981 tgtacgttcg atcagcgagt cacggtcagt gatcacacct cctaaataat aaggcaaagc
     2041 acaacttgca taagaaatat tttctccgcg ttcatagata atgatttcgt gcgcttcatt
     2101 caatcgacgg aaacgagtgg caaatgaagg accgccagct acaccgccaa tgacaacaac
     2161 tttcataaaa acatctcctt ttctacgttg atcgtactat gttatggtaa aaaacagcaa
     2221 ataattgtgc cggaaaatta ttgtttattt tgaatagaca gaaaatatag agaaataatt
     2281 tgtggataaa acctaaaaat ttttttctat tttaaggata gataatactt tgaaggggat
     2341 aaccctgtat attttttaaa agctgaagaa aaatgatgga tcgaatgaaa tcctaatata
     2401 tctgcaattt ctgtataggt taattcattt tctagcacga gacgttttgc tttttccatt
     2461 tttagttctt taaaatagct aataacactt tttccattca cttttctaaa atgttttttt
     2521 aagctggctg gactgataaa aaaaatttgg gctaattgtt caagcaaaaa attttctgaa
     2581 agatgttctt ctaaatatcg tatgattgga tcaactaatt catctgaata aagaatatgc
     2641 ttttttttag gaatgacttt ctcatttgaa ggtattgtca ataaagcaag ttcctgttga
     2701 taatgccttg gtaaggaaga caattgaatt ttttgcagac aataagtaac taataaggaa
     2761 agcaaatgaa tgctcatctg ttggcatagt atgaaaaaag aattgatttc ctgatcatag
     2821 gaaatagctg gcttactatg ttttagataa tcaattttga actgttcatt tttcagaaaa
     2881 gtaatgatag atgttatttc ttgatctata gaaaaagatt gtcccgtaat atgtgctaat
     2941 aattgctcat caaaataaaa ctggataatg ataaaagttt gtgtttcctt ttgagtagaa
     3001 aagatttgga cttcttgctg tttatcaatg aataaaaggg aaggattatc tagttctata
     3061 gatttatgtg caaattcagc atataaatta gcgctttcat ttattagtag tcgaaaatag
     3121 ggagcagcag ataaatgaag atgggtatct atggaatagg ttagtgtttt tttgacaaga
     3181 ttggaggatt ggttgtttgt taacaaaaga accactttcc tttctctttt tatataaaaa
     3241 gctttctatt tataattaat gactattata acaaaaatga aaagccaaaa cgacaagtaa
     3301 aatatttttt tatataaata tttgtgttac taattccgtt aatatacata agtgaaagcg
     3361 ttttatataa gaacttgaaa agaaagaagg gatagacatg aaaaaaagat acaaattggt
     3421 gatagctttg ctaaacatta ccctattatt cagcttaagt gcatgtggaa aaactaacga
     3481 agaagtaaca gcaaacatcg cagatccaat gataaaaaaa gccactacat tagaagtata
     3541 tgtcgcaaac ggcaaactga caaactatgt tgaaaatgct gcaaaaatct ataaccgcaa
     3601 aactggagca aatattgact taaaaattgt gaatgtagct tcgggaacag caacagtaca
     3661 aatgatcacc ccaaaattgg taacacacga agatatgccg gatattatct ccatggcaga
     3721 cacttcagca gcaggtgtgc tggaaaaatt tcaagatgct ttttatagtg ctgatgaata
     3781 tggattttat gacaaatatg gcgatcagtt ttatgatcaa aaattgaata ttttgcgtca
     3841 acaaacgagt aatgaaacga ttattccttg ggctagtgat tttaccccag cactttgtta
     3901 ttatcaacca aaaaaatttg ctgaaatagg tgtagatttt gaagaaatca gttcttggga
     3961 agaatatatc caggtggcaa aagcattaaa agaaaaaact ggtagttttg gaattgcctt
     4021 gccagaatct ggggaccaag aattgtttat caacatgcta gcccagcaga atcaatcttt
     4081 gcttgatgaa gaaggaaata tccgtctgaa tactaaagaa gcgaaacatg ctgcaaagtt
     4141 aatcaaagaa atgatcgact cagatattgt acatttctac ggtgcacaag atgctgagaa
     4201 agcatttcaa gaatctgcaa tgtttgttgc tggcggctgg tatgctacga atatgtcttt
     4261 gaattttcct gatgcatctg acaaatggcg catggctcca ctgattcctt tttcaaaaga
     4321 aaatccagga aaaggtgcag tatcaggagg atcaagtttt tatgtcccta aagatgccaa
     4381 aaatccacaa gtggcacaac aatttcttac atttatgctg acagatgaag aatgcttagc
     4441 caacgcactg gagctgggcg tggctacatc taactcattg gcctatcaaa cagaagcagc
     4501 tgagaaaaag tttgactact aaggaggaca aaaatactat gaactattga ataaaatcaa
     4561 tgaaaataca tcaaatactg ttttaccagc cacttattca gatgctacgt cttatttgac
     4621 taccgctgca tataactatt ggcaaacagg tgattttgaa aattcatatc taaaagaagc
     4681 agaaaatttt gctcataagt acggcgtaaa attaactggt gattaaaaaa tattaggagg
     4741 acttaacgat gatgcaatct tcgttaccaa gcaagacaat aaagaacagc aagaaaaaag
     4801 caacagatac gtctcataaa aaaagtgctt atggattggt cacacctttt gttattttct
     4861 ttattgtgac gattttgatt cccatcatta tctcgtttgc ttttagtttt atgaaaattg
     4921 gctactcatt tgaatttgtt ggatttgaca attatctagc tatcttccga gatccattgt
     4981 ttattaaatc ttacacgaat gttttgatta tcatggcagg atcaattcca ataacgatgc
     5041 tattagctat ttactttgct gttttattga attcagcaca gttaaaagga aaaggatttt
     5101 ttagaactgt ctattatatt ccaaccatta catcaacggt ggctgtagct tccgtattca
     5161 tgactttttt caatccgact gggttattca atggattttt gaatgcgcta ggaattaacg
     5221 gtgtacagtg gttaacaaat cctttttgga tacgaatatc aatgatcatc acgatgattt
     5281 ggcttagtgt aggatataac actattttat ttttagctgg tcttcaaggg atttctaaag
     5341 agatatacga aagtgctgaa atagatggag catcgaaatt tcggcaattc tttttattaa
     5401 cgatccccat gctaaagcca atcattttaa tggccacagt tttagccact atcaatggct
     5461 tgggtaattt caatatccca aatatattct ttgggacttc caatggacca gaaaattcag
     5521 ctttggtcgt aggagtcaat ttatataaaa caagttttga aatggttgat tttggaaaag
     5581 cctctgctat tgcctggacg atggtgtttg tatctgttat attatcggct attcaattta
     5641 aattcggagg tgataaaaat gactaaacaa aagatacaaa gcagaatttt tacgtacgtc
     5701 attattgtgt ttggtgcctt aatatttttg atgccatatt tatatatgat tgtagcttca
     5761 acgcaaagta atcaagaaat tttaagtggc ggaatcaatt tcaagtttgg ggatatgcta
     5821 ttaaaaaact ggcaagccat catggcgaga tataattatc ccttgattat ttggaattcg
     5881 attgttattg ctgtaatcag tacagtttta gctacagcca gttctacaat ggcgggttat
     5941 gcattagcaa agtatcgttt tatggggaat aaactgttgt tcaatacagt tatgctatct
     6001 aggatggtgc ctttttttac cacattgatt cctttatttt acattatgag tcgtttagga
     6061 ttatcgaata cttatgcagg tgtgatcatc ccttcgattg ccagtacaac ttcagtgttt
     6121 atgatgcgac aatatagcag tcaatttcct acagcactga tggaggctgc cagaatcgat
     6181 ggtgccaatg agtgggtgat ttttacgaaa atcgctgtac cgattttatc acctacgatt
     6241 attactagtg gtttacttat ctttatggga agttggaatc aatatctatt tccattagtc
     6301 atgctttcag ataaagataa atatacggtt cccttaatta tcaaaaatat gacggtttct
     6361 agtacaggtg agccaattaa ttatggcgca attatgctaa ttttagcaac ttcagtgatc
     6421 ccaatggtct tgatttatgt gtgggcgcag tctaaattta aggagaatga cattggagct
     6481 gcaaataagt aaaaaaaata acgaatgtta accatctagt tcgttctagg agcaactcca
     6541 cattcataaa aattaaaaga atggcgggat atccatgaat gaacagacga aggaatggtc
     6601 aagaaaaatt ttgaatgtag cttctgaaaa gatagatgcg gaattagaca gaatggggaa
     6661 taaaattcca tatatttctt acaacggaaa atatacagac gtaataaagg acagaggaat
     6721 cgactggtgg accaatggtt tttggagtgg tattttatgg cagctctttc acaagacaaa
     6781 acgagaaaag ttcaaagagg cagcaataca acaagaacaa ttattagaat ctgccctgta
     6841 ccatttttca gacgttcatc atgatgtagg ttttttattc ttaccatcgg ctattgcaca
     6901 ttatcaacag tctcaaaaca aacatagtcg acaactaggg ttacatgcag ctacggtact
     6961 tgctggacgc tataatattt cagggaacta tttagtagca tggaatgacc ataaacctgg
     7021 ttggatgatt attgatagta tgatgaattt accattactg tattgggctt cgagggaaac
     7081 tggtgatccg aggttctcta aaattgctcg gcagcatgca gatacaacag ctaagtatct
     7141 agtgaggaaa gatggatcaa ccgggcatat cgcagaattt gatgcagaat cagggaaata
     7201 tattcggtta cttggcgggc aaggatacaa tgagaattct tgttggagca gaggaaatgc
     7261 atgggcttta tatggattcg cgttaagtta tcgttacaca aaaaatccgt actatctagc
     7321 tatagcagaa aagatagcag taaattttat tcagaaaatc gaaaaaactg gttatattcc
     7381 tctcattgat tttgctgtaa aagattcgaa tattgatacc agtgctggag tatgcgctgc
     7441 atgcggtctt ctggaattag caggatatgt ttcctctgta cgatcaaaaa tgtatcatga
     7501 agctgctatc aatatactcc aaaagacaga aaagcactat gcaaattgga atatggatga
     7561 agatgggatc attggaggcg gagcagaagc ataccataga gaactgactt ataatgtgcc
     7621 actgatctat tctgattatt tttttattga aggcttactt cgattagaac agtcggaatt
     7681 agctatttgg tagtaaaaag aggagaaaat acaatgaaac atccagtacc actattaagg
     7741 accagacagg attactttga cttatttaaa aagataaatc aaccactcca gccgttttat
     7801 cgagcaagtg gcgcacaaat tgattatata catcaagggg taggatatgg aaataaaatt
     7861 gcaggaatgg aaggattctc tcgggtttta tggggagcgg ggcccgcaat tgaccaatta
     7921 gatgaacgat ggttgaaatt aattctatca ggaataaaag ctggaacaga tccttcccat
     7981 cccgattatt ggggagaaat acatgaaaga gaccagcgta tggtagaaat gcccgcaatg
     8041 ttattagcgt tatatcatca tgaacaatta ttatggaaaa aattagatac aggagaaaaa
     8101 agccagatta ttcattggtt ctcgcaaatt tctgaatatg aatgtgcaga tggtaattgg
     8161 caatttttta agataattgt cggaacaatt atccaaaaat ttggttatcc agttaaagaa
     8221 tcagcactga aagaagcatt tgataatata gaaaattgtt atcttcaaaa tggctggtat
     8281 cgagacagtt caagaggtcg ccaagattat tataatcctt ttgcatttca ttattatggt
     8341 ttgatttata gcgtactaga accagaaaag cccattagcc agatatataa gaaccgagct
     8401 attgattttt ctagagacta tattcatttt tttgcggaag atggtgcaaa tgtgcctttt
     8461 ggtcgaagca tgatttatcg ttttgcagtt tcaagctttt ggtcagctat gttgtgggcc
     8521 gatcttttgc cttttaaatt aggtgaaatc aaggggttag tcaatcggaa tataagatgg
     8581 tggatgcaaa aagaaatatt tgatgaaaaa ggtatcctga atattggtta tacctaccct
     8641 caattgacat tgactgaacc ctataatagt acgttatctc cattatggct gaataagatt
     8701 tttttattgt tggcgctacc tgaagaccat gagtattgga cggctaaaga aatgcctatg
     8761 cctattcgtg aaggaactaa acttctttca gaagccaatt tgctcataca acatgataat
     8821 ggacatacat tttttttgaa tgcgggacaa ttagggccaa atttccatac attgaccaat
     8881 gaaaaatatt taaaatttgc ttattctagt caatttggtt tttcgatacc gcgagcgaat
     8941 caattgaaag cagaaatggc aatggattca atgctaggga ttcagcgagc ggacacatat
     9001 atcacgacca gttttaagaa tgagagcatc aaaacctatg gacagttcat cgttcgaaat
     9061 aatgtacgag acattgtatg taagaaagag gtacttgcct ctacatggga acctacttta
     9121 caggcaaaaa tcagaacttg gcttattcct tttgaaggct ggcagattcg cgtacacaaa
     9181 gtagaattag atacagaata tgttctatat gagacagggt ttgctattga atgttctcct
     9241 gagaaagaag gaataatcat agaagaagac aaggaaaact atagggtgag tgaagctggc
     9301 ttttcaggta ttatctgttt aagtgacgat actattaaac gaaaatccac agctattatg
     9361 ggtttgccca atacaaactt gatgacatgg gaaaacactt ttattcctgg attagagggt
     9421 acatttggaa aaggaaccca ttggctcgga acaggagtgg ttgctcatca agatcgggat
     9481 tatagtttag aagtatggaa caatcgtccc aaaattgatt ttgatgggac aaccgggcta
     9541 actatccaaa ataaaaacaa taaaaaaaga ctaaaattat gctaaaggct ggtggggaaa
     9601 tgcttgataa agtgatgttt gaagagataa tacatccaga aaaatggata aatgaatgtt
     9661 ataaagaatt aatggaaaaa gaatggaata atgagatcga gcgttgtcgc agacacacgg
     9721 agaggttaat caaacgtatc agtaacagtc agtatcaatt cccaagcgag tgtcctataa
     9781 aaggacgtta tccattaaga gacaatattg gttggacaga aggattttgg acaggaatta
     9841 attggttgtt atttgagaag acgggtgatc ctttatttca acaaacagct gaaaaagcag
     9901 atatagattt tatccacagg attgactgtc aaattatcac taatcatcac gatcttggtt
     9961 ttttgtattc tttatcaacc gtagcagatt ataaaataac gggaaatcga aaaatgaagg
    10021 aagcttcaat tgatgctgca gaaacattac ttcagcgttt ttgggagaag gcaggagtta
    10081 tacaagcttg gggagatcct agtgatcctt cacaacaagg tagaatgatc atggattgtt
    10141 gcatgaatct tccattattg tattgggcga gtgaagaaac tggaaatcaa aaatataaaa
    10201 aagctgctca ggctcatatt caaaatgcag cagattattt agtaagagaa gatggttcaa
    10261 cttatcatac attttatatg gatgtcaaag atggaaaacc aagatatgga agtacgcatc
    10321 aaggttatag tgataattcc tgttgggcta gaggccaagc atgggggatt tacggttttg
    10381 cattaagtta tcgttatttg aaagaacaaa aatattttga tttaagcaaa atattagcca
    10441 attattttat gaatcggttg ccagaagata ccatttgtta ttgggatttg atctttgatg
    10501 attctagcag agaagaaaaa gatacatcta gtatggtgat catggtttgt ggattattag
    10561 aattaataga atcaggtcta ttagacaaaa cagaaaagaa cttataccag tctgctgttt
    10621 ggaaaattat gcgacaattg aaagaaagat atgctacaac ggaagaagaa gatggctttt
    10681 taacacagtc tgtttataat aagcccaaaa ataatggggt aaatacacct tgtatttggg
    10741 gtgattacta ttatttagaa gctttagtga gaatgactag taattggtct tcttattggt
    10801 gaaaaaggta aatatggaat ttttgtttga taaaataaaa aaagaaaaac caaaacagca
    10861 tgaatggttt ctgttttggt tttttcgtta attttcatat tcttctaatt ccaagcttag
    10921 ttcttcccat tccgtcaatt ttgtttcttg atcttgtcta gcagcttcta aatcttggtt
    10981 cagctcattg agttttacgt gatcttccag gttttctggc gcgaccattt cttgttccaa
    11041 ctgatcgatc cattcatcga tccgcgccat ttcttcttca acctgagtaa tttttcgttg
    11101 aaggctacgg ataagctttt gctgttcttt gctttggtaa taatcagatt tgttcgtggg
    11161 agcaggagca gattctggct catcttgtgc caacaattcg gcgatttctt cttcttcctg
    11221 ctttttctct aaataataat catagtctcc tagatagact ttgctgccat tttcagagag
    11281 ttcgacgact tttgtcgcga tccgattgat gaaatatcgg tcatgggaaa caaaaagcag
    11341 tgtgccgtca taatcgatca atgcattttc taatacttct ttgttgtcaa tatctaaatg
    11401 gtttgttggt tcatccaaaa tcaaaaaatt ttctttgttc atcgctaact tggctaaagc
    11461 aacccgtgct ttttcaccgc cacttagcag agggattggt ttttctacat cttcgccaga
    11521 aaataagaaa gcacctaaag cacttcggat ctctttttct ggagtggtag gatgctcatc
    11581 ccacaattct gctaggatcg tcttgtttgt atgcagatca gcttgacctt gatcgtaata
    11641 gccgatggtg acatttgtcc ctagcgtttc ttttccacgg ataaaaggga tctggtgaat
    11701 gattgatttc agcaacgtcg atttcccgat accattcgga cctactagag caatcgcttc
    11761 ttgacgacgg atatctagat tgattggttc agataaggtg acattatcat atccgatagc
    11821 ggcatcttcg atttgcaaga ctacatttcc agaaggcttt tctatactga atagaaagtt
    11881 tgcttgtttt tcatcccctt gaggacgatc cagccggtcc attttttcta atgtctttct
    11941 gcggctttgc gctcgttttg tggtagaggc gcgaacaagg ttccgagcaa cgaagtcttc
    12001 taatttatgg atttctgttt gctgcttttc ataagccttc cattcacttg ctaactgttc
    12061 tgctttcaaa tctagatatt tcgaatagtt acctttgtaa tggcgcattt ttcttctgct
    12121 taattcatag acttcattga ctactttatc taaaaaataa cggtcatggg aaacaatcaa
    12181 taatgcacca gtatagcttt gcagataatt ctcaagccag gaaagtgtct cgatatctaa
    12241 atggttcgtt ggttcatcta gaatcaaaat gtcagggtgt tgcaaaagca tgcgagctaa
    12301 agctaaacgt gtgcgctgtc cgcctgataa agtatcgatt ttctgatcat aaaatgaaga
    12361 atcgaattta aacccatgca atacggaacg aatttcattt tcataaccat agccgtttcg
    12421 atctgaaaaa tcgtgttgca gccggtcgta ttcttttaaa taagaactat aagcaggtgt
    12481 gtctggcgta ccttcgctaa tgaaaatctc tagctcgcgc atccgctctt ccatttgtct
    12541 aacttgttca aaagctttca gcatctcgtt ccaaactgtt tcattggaat caagtcctgt
    12601 gtcctgagcg aggtatccga gagtcgctgt tttattttta gcgatctttc cttcatcagg
    12661 tgcttcaatt ccagcaatga tctttaataa agtcgatttt ccagcgccat ttctgccaac
    12721 taaagcgaca cgagcgcctg tactgatttc taaatgtatg ttttcaaata agacttctgc
    12781 accaaaatgg cgagcaatct gattggcttg taataatatc atggaatcct cttttctact
    12841 ttgtgaattc tcctcaaata aaaaactatt tttcaattag tagtctacca taaattccat
    12901 tatactgcga gatttgcaga aatgataaat aaatgaataa agaataaaat ttcacaatat
    12961 ctgagcaatg gaattgattt tttcctataa atactgctat gatggagaaa tcaaagatgt
    13021 aattagacaa tacgtggagg tcatttcgtg aaagatcata ttattccaaa ggcgacagct
    13081 aaaagactgc ctttgtatta tcgctattta aggattttaa ataatgctgg aaagaaaaaa
    13141 gtgtcttcaa ctgaattaag tgaagccgtt caagtagata gtgcaacgat ccgtcgagat
    13201 ttttcttatt ttggcgaatt aggaaaacgt gggtatggtt atgatgtaga agatcttatg
    13261 cacttttttg caaagacatt aaacgacgat caattgacaa atgttgcgct gatcggtgta
    13321 gggaacttgg gaagtgcttt actaaaatac aaattccatc aaagtaatag catacgtatc
    13381 agttgtgctt ttgatgtgaa tgaggaaatc gttgggcgca tcgtagacgg tatccccgtt
    13441 tatccaatgg aagatatgtc agaacaaatc aagatccaac aaatcgaagt agcaatttta
    13501 acgatacccg ctgaaagagc acaagaagta gtcaatcagt taacggaagc aggaatcaaa
    13561 ggaatcttga actttaccgc tgctcgattg aatgctccgt cagatgtatt gatccaaaat
    13621 gttgatttga cgaatgaatt gcaaaccttg atctatttct tgcattctga cgcaaatgcg
    13681 cattccgaaa atacagcaga ataaaaataa aaagcgattg aacaaatgaa ggcaaaactt
    13741 tcacttgttc aatcgctttt ttctgtttgg acaacgatta ttttgttcgg aagatttctt
    13801 cataagaaag ggaatcatct tctgctgtca gttcatctag tagataacgg atttttttaa
    13861 agtcgatgaa ccgtgcttgc cgaaagactt cttttccttg atgaaaaatc aggacaactg
    13921 gcgcagtcag cacttcaaat ttactagcta cttctggtgt ttcaaatgca tccaaatgaa
    13981 gtgccggaat atcataatgt gtgagaagtt cctctagtcg tggacgaaca gcgtgacaaa
    14041 cactgcaatt aggcatcgaa agataaatga cggcaacgga tgactgttgc agtatgtctt
    14101 gaacttcagt caagttagtg gtttgtttca tttttattct cccttttatg atagaagtga
    14161 tatgtatgag accttaattt aatgggaata acagaaaaag tcaattgaag tgctttggta
    14221 aaaaataaga gttgtgtaaa aatttacaga gcaacttgtt ttttttgaat aaaagcgtat
    14281 atttgcaaag cgaatgaact agaaataaaa aggaggaaac tcatgttttc ttttattcca
    14341 aattcggttt tgcagatgag tacgattttc ttatcgatcg ttattgaagc attacctttc
    14401 gtattattag gctgtcttat ctcaggcgcc ttacaagtat ttttgacccc agaacgtgtg
    14461 aagagatggc ttcctgaaaa tcgtatcttc tctattttta ctggttcgat cttaggattt
    14521 ttctttcctt catgtgaatg cggcatcgta ccgatcgttc atcaatttgt taaaaaaggc
    14581 gtaccagttc acacagcgtt tgcttttatg ctgactgctc cgatcatcaa tccaatcgtg
    14641 attttttcca cttttatcgc atttggcaat tcttggcaga tggcaagctg gcgggtgata
    14701 ggcagctttt ttgttgccct tgtgattggt gtctggcttg cctattttca aaaagacgct
    14761 gtattgaaag aaaaaatcac gtctaaagta gcagaaaatc accatcatca tgtccatgaa
    14821 gaagaacaaa gtgagaagaa tgaaccaaaa aatcagctga tccatcaaac tgggcatgtg
    14881 ttgacacatt caatcgacga attttttgat acaggtcgtt atttgatcat tggcggatta
    14941 atcgcagctg cgatgcagac ttatttacca actagaatta tgctaacgct cggctcaaca
    15001 aagattttag ccattatcat catgctgtta ttggccttta cgatgtctct ttgctcagaa
    15061 gcagatgcgt ttataggatc ttctttgtta agtctgttcg gcacagcacc tgtagctgcc
    15121 tttttggtct ttggcccaat ggtcgatatc aaaaatctac tgatgatgaa acgatatttt
    15181 acaggacgct ttattttatc gctgatcgga ctgattgccc ttagcgttat tggattcaca
    15241 ttatttattt aggagggaaa atatgctgcg gtttttgatt ttatctgggt atttcgaatt
    15301 gatgatgtac ttgcaagttt ctggcaaact cgatcaatat atcaatgtcc attatcgcta
    15361 cttagctatt ctttcaatga ttttatctgc tcttttggct ttagttcagc tgtatttgtg
    15421 ggtcaaaaac ggaaaagcag aagaaaaggc acacgagcac gatcatgacc atgggttgtc
    15481 aaaaccttat caacgtggaa tcgcttatgt actgctagct ttgccagtaa tcgtggggac
    15541 gttgtttcca acagttagtc tcgacactac gatcgtagaa gccaaaggat ttaatttccc
    15601 agtaagtaaa gaatcagtag gggatccgga gatgcagaca caatacttaa agccggatac
    15661 cagtatttat ttcaacaaat cagactactt agaccaaatg aatgagttga aagaaaagta
    15721 tggggataag caaactatca agatcacaga tgaaaactat ttagaagtta tggagctaat
    15781 ttataattac cctagcgaat tcattggcaa aaaaatcagc tatgaaggat ttgtttacca
    15841 aacaccgaac gatagcaata cagatatctt cttgttccga ttcggcatta tccattgtgt
    15901 ggcagattca ggagtctttg gtttgttgac gcatatgccg gaaggagtaa cggttaaaaa
    15961 tgatgaatgg tataaaatag aaggaaccat ccaatcagac tattatcaac cattcaagcg
    16021 tgaaatccca tctgtagttg ttacaaacgc tgagaaagtc tctgctccga aaaatcagta
    16081 tgtgtatcgt tctttttaaa agaacaaaat aaataaacat agctcagaca gctgacacgc
    16141 aaggacgtcg gttgtctggc tttttttgca tgatgtattc atttgtttga tatttatttt
    16201 agaagcagca tcctgaaagt atttgatatc ggtttcaaag gtgttggaaa agattgtgaa
    16261 gagaattaca atttattcct aacatgtatg ttgaaaagtt gtatgaaatg attttgatag
    16321 taaaatatcg agtggaggta ctttttatgg taacgcgtat atttattttg gatgatagtt
    16381 tggtttttga aaaaatgcta gtagaaattt tggaagaatc cagaatttta ttaagtcctt
    16441 ggaaatttga gatcgttcag ggattgaacg ttatacaatt tgttgaattc attaaaaata
    16501 atgaaatcta ttcctcagat atttttttcc tagacatcga tttgaacgcc tattatagcg
    16561 gtatcgaact aggcgaaatg cttagagaaa agaatgaaaa gtgtaaaatt gtttatctaa
    16621 cgtctcatcc gaataaagca ataactgtta ttaatcgaaa gattacgcct agtggttatt
    16681 tgataaaaga attcacttat gaaaaaaata aaaaaagcat ccaaagttac ttattgaaag
    16741 aaaaagaaaa ctatcctgtt caacatgcta cgaaaaaaag gtggacaact gtccgttatg
    16801 gtaatagcga tcactatatc cattacgatg aaatccttta tctaatgagc atgccaggtt
    16861 ataaaaataa aataggccta gtattgaaag atgaagagat tctcgtaaac ggcacaatta
    16921 aaaaatataa aaaatcactc gaatatgagt atttatgtaa agatttaaaa gcttatatca
    16981 ttaatactga aaacattaca tctattagca gagtaaacga aatagtaaca tttgcaaatg
    17041 gagaacaact agagatggga aagagaagta ttgataaact aaagaatttt atgcaagaaa
    17101 agatagaaaa tgaatcattt taaagaaaac aaagactcat atcttacctg ttagaagagt
    17161 gttttcctgt agaagagaac aacccaagta gcagcctagt cgtttctatc cttttttgtt
    17221 atgcactctt tttgacattt actccaccaa taacgaaggg tacaataaaa agtaataaca
    17281 aacaaaagga gtacagaaat gattgaattc atcaatgtaa gtaaaagtta cgggaaaaaa
    17341 caagcattaa aagacttgtc tctctcgata agacaagggg aaatttttgg atttttagga
    17401 cataatggtg ccggaaaatc aactacgatt aaaagtttag ttagtattat tgaaccatca
    17461 tcgggaacga ttttagtaga tggaatgaaa ttgacggaaa atcgattgag tatcaaacaa
    17521 aaaatcggtt atgtgcctga ttccccagat atttttttac aattgacggc tggagaatat
    17581 tgggacttga tttcagctgc ctatgaatta gacacacaaa aaaaagaaaa gcgtttagca
    17641 gaattgacag cgctttttga tatgtatagt catcaaaatg aaacaatcgc aagtttttca
    17701 catggaatgc gtcaaaaaac gattttgatc ggcacgttgc tgccagatcc agatatttgg
    17761 gtattggatg aacctttgca aggacttgat cctcaagcag catttgattt gaaagaaatg
    17821 atgaaggctc atgccgcaaa aggaaaaaca gtcatttttt ctacacatgt attggatacc
    17881 gcacaacagt tatgcgataa actggctatt ttgaagaaag gtgaattgat ttatcagggg
    17941 gcagttagtg aattattgaa tggctcacct gatgaaacgc tagaaaagat ttatttgaaa
    18001 atggctggaa ggcaagcgac aggagatgga ccgtatgtat aagcgacaat tattagaatt
    18061 aattcgagtt aatcttttat atgttaatcc acagctcacg aataaagcaa gagagaaagg
    18121 aaaaaaggga aagcgattaa ctatctatct tttgtctcaa aatctttttt cagggctgat
    18181 ttttttactg atttacggat ttgctatgtt tgctatgaac tttgttaaat tgccaggttt
    18241 ctttacttat tacgtggcac tttttggtat gttaggttta actcaaagta tatcagtcat
    18301 ttataatatc ttttttgaag gaaatgacct gcaagtattt ttgcctctgc cttttggaca
    18361 agaacaggtt ttttcagcaa agattttggt agttgctttg accatcattc cttttgtttt
    18421 tccgcttcta gtcatgttta ttttaaccgg atggcgagct gatctaggga ttttgcttac
    18481 tatcattatc tcaatccttt tgttttctct atttttgatt attctttttt gtctggctag
    18541 tttgattgtt tttggtttgg ctcgaacagc agtgtttaaa aaacatagaa aaacagtttc
    18601 tagtatcttg cttggcagct cgatgctgat cgcatttgct ggaatcatgt ggatgaatca
    18661 tcaaacgggg actattgaaa atgagttaac agatcggcat ccgatttcga tattattgcc
    18721 attctatgat gctgccagtc agccactttc tcaacaaggt ttattgggtt ggggaatact
    18781 agctatcacg gctctgggat ttttccttgc tgtaaaacag ctgatagtac caaagcttac
    18841 agagcaactg actgattcaa acagtgagtt caaaataaaa agaacccata aaaagaacca
    18901 aaatgtgcat cagcttttat ttagttacaa tgctcaatta gtaaaagaac ctaatttgat
    18961 tatgcaagtt ctttcttctt ccttgttaac tccaatcatt tttattcttg cttttgccct
    19021 aggaggcgaa ataaaattaa ctgatttaga taataagtta atcggtgttg ttttcctggt
    19081 agggatcgca ttggctttct taacagttaa tcagacttct tttatcagca atcttatttc
    19141 tttggatcaa gaaaattttc tgtttatccg ttctttgcca atctctatga accaatattt
    19201 aaaagaaaag ttcagattcg gatggatatt gcagagtttt ttaactggtg ggatcgcatt
    19261 attaagcgga ctgtcttttc agctgccttt gttttttatc cctagcttgc tatttggttc
    19321 tttattcggc tgctacttat tgtcactacg ttatttcgct agagattatc ggttgctgct
    19381 gctgaactgg accaatatta accaattatt taacagaggt tcaggaagtt tgggtttagt
    19441 cgcaactatg atgggttcat tgatcatcag tattattctt atcgtcttat acggaatgtt
    19501 ggctttgttt tatccatttt ggttagtgaa tattcctgtg atcggtttag ttctaatact
    19561 ttccgctcta tggatcgttt attatcaacg aacgttttgg aaaaaatttg aataaagaag
    19621 aggggccgtg acaaaaatca tgtcgcagcc ccttttttac agatagaggg tgttcaaaag
    19681 tcagtttctc agtattaagt caattttatg taaaaatgtg agaagctatt tttacaaaac
    19741 tttttttaat actcataatt acttcttatt gctcctgcgg ttacttgtcg caatgtttgt
    19801 gagatatttt gtcatcgctc aaggctgaac gtctctttca caaccttttc tttgttacac
    19861 ttcgtttatt taaaaagccg tttttttcgt ttcatcatga ttgcgtcacg ctttactttg
    19921 aaattacgga tgttatggtt aaagacttct gataatacaa gacctaaagc gattgctcca
    19981 gcaatcataa ttgcttggac cgcataatga gccgcttctc gataggattc agtaacgaga
    20041 tttcgaaccg cttgataagc taaaccacca gggactagag gaaccatccc gggaatattg
    20101 aaaatcgtta cgggcatttt caaacgttta gagaaaagat cgctgatgaa tgctactcct
    20161 aaagaaccaa gcaatgaacc aaaagcagca gatccgccta tttgaacaca gatccagtag
    20221 atcatccagc caaaagcacc agaaagacca caacaaatca atgaacgcct aggtacattt
    20281 gtaataacgg caaatgccgc agtcgctaaa aaactaaaag aaaactgaat aaatatcgtc
    20341 cccatacttg tgttcctttc ctaataaaaa agctggaaaa taaaagcaat cgcaaaaccg
    20401 atcatcgtag cagtcatcat cgcttctgtt ccgcgagaaa ctcctgagac ataatgtcca
    20461 gctagcaaat cacggatagc gttcgtaatt tgaactccag gaactaaagg catcacacat
    20521 ccgataatga ttagatcctg attgcttcct aagccaaaac gtacgctgta gatagcggct
    20581 atgccaatca ataaagaaga taaaaattcc gataaaaatt tgattctaaa aaatttcaaa
    20641 ctatatagat aagctgcgta tcccaatcct ccaatgacta aagtcacagg taaatcggtc
    20701 atctttccgc caaataacac cataattgtt ccactgatca ctgccgcgct aacgatttgc
    20761 aaccagacag gaaagaaaga acggtctcgg tcgattgctt gcagttcgca gtaaagttct
    20821 tctaatgtta gctctgaagc aacatattta cgtgaaagat catttacttt tactactttt
    20881 tctaaattaa tcgaacggtt ggtaatttgt tccattcgga tggtcgaggt acgatcaaga
    20941 ccaataaaga gaccggtttg agtgacatag ctgaccaaac ggaaatttcc agaagctaaa
    21001 gcaattcgac tcatcgtatc ttctacccga tacatctccg cattgctttc catcatgatt
    21061 ttcccagcga gtaaacaagt gtccagcaac agatctaatt cttttcccgt catagcatct
    21121 tctccaataa ttgattgatt agttttattt tactacaaaa cataccagaa gcgtaaagaa
    21181 taaaaaaact tgaggaagga ataaaagagg cgtatactgt ttttgtattg cgggtgtagt
    21241 ttagtggtaa aatcccagct tcccaagctg atgtcgcggg ttcgattccc gtcacccgct
    21301 taaaataggt tgtgaaaaag ctgctctgca acaagtaata agaacagcca agcaaaaaca
    21361 acttcttatg tttttaactt ggatggactt attaccgcag atgcaagctt ttgcgcaccg
    21421 tttaccaagc ggttgtgaaa tcagtgtatt gtcttgagta tgaagaaaat ttttgaataa
    21481 taataaatac cttacagata gagtggtgac tgattagaga cgaggaatcc gtctcttttt
    21541 tgtattttta agccatctta aaacaggttt tattttttgc tgtttctatt tctttctagt
    21601 atttatttat aggggaacat atgttaagat ttagggagct gttagtaaag gagtaaagaa
    21661 atgagcaaaa aaacaaccaa ggcgatcgtg gggatcgtcg ctgctgtgat tgcaggggca
    21721 ttcggtgttt ccaccacaca aaaagattca gatatctttc agcagattac aactattttt
    21781 gattccacac cacaagcttc tcaagtagcg gatgtgccag aatatgatgg aaaacatcaa
    21841 gaaatagaaa tcaatgggaa tcagccggaa tttacaaaag aggatcttag cctttccaaa
    21901 ggaacatggg aaagctattc aaatattgat aaattgaaca gagtaggaca agccaatgct
    21961 atgcttggaa aagatttgtt ccctactgaa aagagagagc ctttgtatat tgacccgaca
    22021 ggttggaaac aaaagaaatt aagtgacggg caatggttat ataatcgcag ccatttaatt
    22081 ggtttccagc tgactggtca aaataacaac attaaaaact tgatgacagg gacacgttcg
    22141 ttgaatgctc catacatgct tgctcacgaa aatgatattg cggcatatat caaagaaaca
    22201 aatcatcatg tacgctaccg tgtcactcct cattttgagg ggaatgaact ggtagccagg
    22261 ggagtgcaat tggaagcaga aagcattgaa gacaaaaaaa ttgaattcaa tgtctttatc
    22321 tacaatgttc aagatggcta cacgatcaat tatgagacag gacaagcaac aaaaacaaaa
    22381 aattagtagc agctattcga attttttatg aatattatga aaaaattgtg taaaaacata
    22441 cattcaaaga aatatagcgt atactttatg aaaagagtac ttttgataaa gctacagaaa
    22501 aaaggaaaaa aacatacaaa tagtgtttct taatttattc ggaagtttgg aatatttgtt
    22561 tgtagttttc cctttcttcg agtaaaataa ggttgtagaa ggtaagctgg aattaccttg
    22621 cgggaaagag gtgtagaatc atgggtttta cagatgaaac cgtacgtttt ggttttgacg
    22681 atagtcgcaa aaaagaagtg agcgaaacat tagcgattgt ttatcgggct ttggaagaaa
    22741 aaggctacaa tccaatcaat cagattgtcg gctacttgct ttctggagac ccggcctaca
    22801 ttcctcgtta tcgtgatgct cgaaatttga ttcgccgtca cgaacgcgat gagatcatgg
    22861 aagtaattgt caaggactac ctctcaaatc atggagtgaa tttatgagag taatgggctt
    22921 ggatgtcggt tctaagacag taggtgttgc tatcagtgac ccctttggct ggacagctca
    22981 aggagtagag atcattcgga tcaatgaagc agaagaagag tttggctttg atcggttgaa
    23041 ggaattagta gcacaatatg aagtagaccg gtttgtagtt ggattaccta aaaatatgaa
    23101 caattccatc ggaccaaggg cagaagcttc catgtcatac ggagaaaaga tcaaagaact
    23161 attctccttg ccagttgatt atcaagatga acgattaacc actgttcagg cagaacgtat
    23221 gctagtagag caggcaaata cttcaagagc caaacgaaaa aaagtcatcg ataaattagc
    23281 agctgtgatg atcttacaaa attatttaga cggctcaggt aagcaattat tttaataata
    23341 aaagaaaaga ggtaatctta tgacagaaca taaccattcc cacgaccatg aaggacacga
    23401 acacatcaca ttggtggatg accaaggaaa tgaaacatta tatgaaatcc tactaacagt
    23461 tgacggacaa gaagaatttg gacgtaacta tgttctttta tatcctgctg gtgtttcaga
    23521 agatgaagat gtagaattac aagcttatgc atacatcgaa aacgaagatg gaacagaagg
    23581 cgagttagaa caaatcgaaa cagacgcaga atgggacatg attgaagaag tattcaatac
    23641 attcatggcg gaagaagaag aatagaacaa aagaatgaat taataagata taaaaaaagg
    23701 gattgtgaca aacgtcttgt cacaatccct ttttccaaat aaacgatgtt caatcagctg
    23761 cccctcggca ttcgctcgaa aaacggcgaa atatgaagaa ctattttgcc atttgtttct
    23821 ctaataatca ggtcaaaacg ttgacttcac agtctcttcc ttaaacggtg ttctcctgcc
    23881 aactcctcgg tcacaagtca caatattcaa caaacatgag aagctgtttt ttgaaattgt
    23941 tccttgtgac tcggagctga acggcaggct cacgacctct ttcttaaacg gtgttcaaaa
    24001 gcttgcatct gcggtaataa gttcaaccaa acgaaaatta tgaggagcta tttttgtttg
    24061 gctgttctta ttacttgatg caggacagct ttttcacaac ctcttttcgt ttagcactaa
    24121 atttccttaa aaagtagaaa agctttcgaa gcctcgtttg tttcttttac tgtataataa
    24181 aagaaaagag taacaatcgc taaaagatat aaacaagaga aaggaaatac aagatgattg
    24241 gtctgattta ttttgtggtg atcgttctgg cgaacacagt tggcgctatt tccgggatgg
    24301 gtggaggcgt gttgatcaaa ccgattcttg atcttatcgg cgcccattct gttgcaggga
    24361 tttcttttta ctcaacagtt gcggtattca cgatgtccat tgtttcgact gtgcgacaag
    24421 tatcaagcgg gaaatcactg aattggcaga ttgtcggttg ggtatctagt ggagctgttg
    24481 ttggaggagt agtcggcaac attgtattcg aagttttttt acagttattt gaaaacgaga
    24541 agcatgtgca gctgatccaa atttttttga cggtattgac cttggttttt gcgttcttct
    24601 acaccaagca tcatcagcca aaatttcatt tgacgtcatg gacttggtac cttatatgtg
    24661 gggggggttc tgggcttttt agctagtttt ttaggtattg gtggtggacc agtcaatgtt
    24721 tctttgttga tgctgatgtt tgcattgcca ataaaagaag caacactata ttctctatct
    24781 acgattttct tttctcaact ggcaaaattg gtaacgattg ctttaacaag tagctttatg
    24841 cgctttgatt tatctatgtt gttttatgtt atccccgctg ctgttattgg aggtttgtgg
    24901 ggagcaagat ttagcagggt tttgtctcca aaaaaagtga cctttatttt tcaagtaatt
    24961 gtcctcgtgg ttcttttggt caattgttac aatgcctttg tcatcttaca aacattagga
    25021 gaatctagac taacgcaagt caaaatcact gaaacatatt gttctaaatg aaaaaaagag
    25081 tatactatga taaaaaaatt cattttaaaa aatcagaaat tggctggtga tttggatgtt
    25141 acgggaaatg cgtttagaag atgcagaagc actacgtgaa atcaatgcgg atcaattagg
    25201 gtatgatata ccattaaccg ttacggtgag acaaatgaag aaactgctga aggagccaga
    25261 gaaaaatttt ttcttggttt atgaagatcc tacttctaaa aaagcagctg gctatgtaca
    25321 agcggagtcc tatgaatcga tttttagcga tccaatgttc aatattatgg cacttgctgt
    25381 gtctaaatat gctgaaaaaa gaggcatcgg aaaggctttg atgactggcg tagaagaaga
    25441 agcgaaaaaa cgaggaataa cgacaataag gcttaattct gcagaataca gagtagaagc
    25501 acaccaattt tatgaacaca tcggttatca ttccgataaa atgcaaaaaa gatttttgaa
    25561 aatcctgtaa atctttatca aaaaaatacc cgatcaccaa gccgatactt gatgatcagg
    25621 tatttttgtt tttttgacta agaaagtaat tgcatcaaga aaccagagat gatcaaaacg
    25681 atggcaccac caagtcgatt gcccatttga gcaaaagcaa taagttccat ccgattagct
    25741 gcagataaga cggctacatt tcctgttccg cccatcgagt tgttgcataa gccagcagta
    25801 atagttgatt caattggata taaaccaaac aaacgaccga caaatcctga aacaactgaa
    25861 atcgcgataa cacttgtcag acataagaca acaaattgcc atgtcaaagc agaagctaat
    25921 acattcagat tcagcaacgc aataccgata ccggcaagaa cagctgctgt caagtttttg
    25981 acaatcaagt tattgaacat gatagctgca tcttcataat actctggtaa taggttggcg
    26041 attttacaga tgacaacgat aataatcata aatgcgtaag catggacttt aggaacaaaa
    26101 tagttaccaa tttgtcctaa gatgaagaaa gtcaatgata ccatcaaacc aacacctaac
    26161 tgtggaatgg aaggtttcac agttcgaggt tttccatcgc caagatcgcc tttttccatt
    26221 aatttaccat tgccgttgaa ttttggcatt gattgtccag cacgagcaac taatgctgca
    26281 ccgatgatag ctaagacatt tgtcattgct gtcgcaggaa tcaggcggga aatgatagaa
    26341 gaagcatccg tacctaaaac atttgcataa atcgttgata aaggaacggc tccggctcca
    26401 acaccaccag ccatcgcagg taatgagatg tataaaacgg aatttgcaaa accatttccg
    26461 ataagcatgc ccacgagtcc tacggcaaaa aaggagacga ccattgaaat aagtgcaacg
    26521 ggaataaaac gaacagaagc tttcatcaat agattacggt tcataccaag gatgctacca
    26581 gtaatcaagg cagcaatata aaagtcccaa aagcccatat tgctgacaaa gttttctgtg
    26641 gatttcacta catattcagg taagatccca aatgtagcaa tcgcagcaga agcgaagatc
    26701 gcaaaaacgg aaccaccacc caaataactt ttgacgatag ggatttttgt cccgataaaa
    26761 tgaaataaat taccgaaaat aaccaaaact gcgattggtc caatcatatt cgtcggtaat
    26821 ttccctaaag ccattgcaat gatcaggaca aggaccatga tcaaataaag tggcaaactt
    26881 acgccggtga tttttgtgga ccaaaaacct tcttttttct tttcctcaac tgcagttgca
    26941 gttgttgttt tttgtgccat ttttacagat cctttcttat tctgtatcat atgaaaaaag
    27001 ccaaagaaaa gcgacaaaag agcaggagtt ggctggccgc tgttttgccg cagtcgcttt
    27061 tttcttttta gacgtcatac aaagtttctg tatgttcttt tactataacg atcttaatct
    27121 tctaaagatt tatattcagg aacccattta gcagattcaa ctgcttcttt gatatccgtg
    27181 atttcttcac gattcaagtt ttgtgcgaca actgattttg cgacagtttc agcaatcgtt
    27241 tgagaaaatt cagtgatttt tgctactgga ggcaagatag ctgctcctgg ttttgttaca
    27301 tcaacgattc cgcctaatgc tcgactagct tgagaaatga tttcagcatt tactcgtgta
    27361 gctgttgaag caatcaaacc aagtcctaaa cctgggtaca tcaaagcatt atttgcttga
    27421 ccgatttggt aagttacacc attatattca acatcagcag cagggatacc tgttccaact
    27481 aatgcttttc cgtccgtcca ttctattaaa tcttttgctt ttgcttctgc caattttgtt
    27541 ggattagaca aaggcatgat gatcggacgt ggtgtatgag cagccatttc tttcacgatt
    27601 tcttcagtga atgctccagg ttgggtagaa gtaccaatca tgattgtcgg atggatcgct
    27661 tttacaactg cttctaagtt tgtcaattca tcagcattag tgaaatctga gcgtttgcga
    27721 gcgaatggga tttgaccagg agtcaatcct tctgtatctt caaacaataa accttgttta
    27781 tctacttgat agaaatgttg gcgtgcttct tcttcaggta caccttgacg gatcatctca
    27841 tctaaaagga tgtttgcgat acccacacca gcagtaccag caccaaatgt caaaattatt
    27901 tgatctttca attgttcacc ggatatattc aatccgccta atacacctgc taggacaaca
    27961 attcctgtac cttggatatc gtcgttgaaa gtagtgattt gatcttcgta tttttctaag
    28021 atcgctgcag catttcctcg tccaaaatct tcccaatgca ataacaattc tgggaataat
    28081 tcggtagctg attgaacgaa ttgatcgatg aattcatagt aagtttcgcc ttctacacga
    28141 gcatgacgat tgcctaagta taagggatta ttcaataatt cttcattatt agtaccagcg
    28201 tcgatcgata caggcaaaac ttgtgctgga ttaataccag cagcagcagt atagaccatc
    28261 aatttgccga tagcgatgtc aacaccgttc acaccccagt cgcccatacc taagattcct
    28321 tctgcatcag tcacaacaat caaacgaatg tctcgtccat ctgccgcgtt cttcaaactt
    28381 gctttgatgt tttctggttc gtcgattgaa agaaatgcag catcttgcgg tttcaagaaa
    28441 atttcgttgt attgttcgat tgaatccgca acgacaggat cataaacgac aggcatgaat
    28501 tcaactaaat gttcgcccat caatttataa aacaatgtcc gattcgtatt gaatagattc
    28561 attaagaaga tacgtttttc caaatctgtt tgtttgctta agtattgacc ataagcttga
    28621 acagattgtt gttccagtgt ttggacagta cttggaagca taccagtgat tccatatttt
    28681 gcccgttctt cttttgtaaa agctgttcct ttatttagaa aaggattatt taataagttt
    28741 aaccctgtaa gcatgaatga ttcctcctaa gttttaatac aaacgaagta tagaagtcag
    28801 atgatgatat agtcaaaaag agcaggtatg ataaatttta tttatatgta atatagccaa
    28861 aagaaatcaa aatagcagta tttgttcttt ttatatcgac aatccttgct tgaatactat
    28921 gcgtatctct tatatgctag agaaagaaaa gtagaagaat aagggataga tggaatggat
    28981 aaataggatt tctgaggaaa aaagcacatg atagcgttga taggagtcac ctatgtcagg
    29041 tgataaaaat acataagtag aaaattaaga ggaggaagag aaaatgaaag aagttcaatt
    29101 gattagaaaa agcgaactat cagaaggcgg ttgcaatgca tgtggcgtag tagaagcgac
    29161 aagctataca ttaaaattag attccaatca agcaataatc tctgaactga cagtaggcgg
    29221 tcttgtggac agtctggcgc ttgctgaagg ttttactgga gaagatattt atgagatgtt
    29281 ttcagaagtc cgtcagttaa aaaaaggaga aaaatgcata gaggttcatc atgagagccc
    29341 gaatgtgagg ttcaaacgtg gtgataatga gatgatcttc aagaatcatg tgtctgatca
    29401 cactgaacta tatgaaatcg tgaatcaaat actgtctggg atttttgaat taggacctta
    29461 tgagttcaag gaagaaaacg gtaatccaaa attaaatgaa gaatggcaag aaacgattga
    29521 aacacaaaga aacaatccac acttatttca atagtcttcc acagtatttc gtaaaaatgt
    29581 tgatgaaaaa gaaacaccgt gaatcagagg tcgtgaaaaa cctgccctgc atcaagtaat
    29641 aagaacagcc aaacaaaaat agctcctcct atttttcgtt tggttgggct tattaccgca
    29701 gatgcaagtt tttgaacacc gtttaaggaa gagactgtga agtcaacgtt ctgacctgag
    29761 tattagagag acaaatggca aaatagttcc tcatattttg ccattttttg agctaatacc
    29821 gagggtcagc tgattgaaca ccgttttatc gagaggttgt gaaaaagtcg cttagtttcg
    29881 agtagtaaga aaaattttgt gaaaatggct ttccacattt ttgcataaat ttgacttaat
    29941 accgagaaaa tggcttttga acaccgttta ttcaaaaaaa gggactttga cactactttt
    30001 gtcaaagtcc catcggtttt ttatctattt ttattacaat catttcttcc agagtagtga
    30061 catctggaat ggctgaaatg taaacagatt atttgcctgt atcaatgtcg aagagtaata
    30121 taaaaagtgt aaacgctatc atattgttca tcattcaaat tttatagaga gaaggcattg
    30181 agatggaaaa aacatatcaa gcaggtacaa atgaaggaat agtagatttt atcaatatgg
    30241 aagatttaga aatcgcggct tcacaagtga ttccagctgg tggttatggt tacatcagca
    30301 gtggtgctgg cgacttattt acttatcaag aaaatgaacg ggcattcaat catcgtttga
    30361 ttattcctca tgtattaaga gatgtagaat tacccgatac aactacacat tttgacgaag
    30421 aaatgctgac tgcaccaatc atcatggcac cagttgctgc tcatggatta gctcatgtga
    30481 aggcggagaa agcttctgca aaaggtgtag cagattttgg gacgatctat acagcaagtt
    30541 cttacgcttc ttgtacttta gaagagataa gagaagcagg cggggagaaa gcaccgcaat
    30601 ggtttcaatt ttatatgagc aaagataatg gaatcaattt ggatattctt gaagtggcca
    30661 agcgtaatgg agcaaaagca atcgtattga cagcggatgc aacagttggc ggaaaccgtg
    30721 aaacagaccg cagaaacggg tttaccttcc cgctgccaat gccgatcgtt caggcttatc
    30781 aatcaggagt aggccaaacg atggatgctg tatataaatc ttccaaacaa aaacttagtc
    30841 caaaagatgt agaatttatc gctgctcatt ctgatttgcc agtctacgtg aaaggtgttc
    30901 aatcagaaga agatgtctat cgatcattag aatctggtgc tggtggaatt tgggtatcta
    30961 accatggtgg ccgtcaatta gatggggggc ctgctgcgtt tgattcgttg caatatgtgg
    31021 cagaagcagt agataaacgt gtaccaatcg tgtttgacag cggtgtacga cgtggacaac
    31081 atgttttcaa agcaatcgca tcaggtgccg atcttgttgc tattggcaga cccgttattt
    31141 atggattgtc tttaggagga agtacaggag tccatcaagt gtttgatttc tttaaaacag
    31201 aattggaaat ggtcatgcaa ttagctggaa cacaaacagt agaagatatc aaaaaaataa
    31261 aattgcgtga gaatcgtttt atttaagaaa taaatgacga aaaaaatagg agtgcggcag
    31321 atgatgccgt actcctattt ttatagttca aggatgtcac taatagaata atttgaaacg
    31381 aaaaaataaa attgaaatat tttttcatac ttaataaaaa aggaagaata ttcatgtaaa
    31441 cgattttatt gtttttttct cttttataca aaaagggtca taatttcaag taagcggatg
    31501 aattcatgag gattttttgc atactgtctc tactatactt aggtcactaa gaaaaaggag
    31561 tgatttatta tgaaaaagaa catgattggg acattattac tttcaacact tcttctaggc
    31621 ggcttggcaa caccagcttt tgctgagggt caagcgactt caaaaggaga tattaccttt
    31681 actgaaccaa caaataccgt tgagcctctt aaccctactg atccttcaaa accagtagag
    31741 ccagcagatc cggaaaatcc agcaactggc cagacaggtt ctttaacatt ggatgtagtt
    31801 cctgaattgc catttggtac acacgagatt gaaagcggaa caaaaacgta tcaagtagac
    31861 gcttcaaaaa acgatacacc ttatctccaa gtatcagatc gtcgaggtgt aggtgcagat
    31921 ggccaagcac aagggtggaa cgtgacagtt tcggtttcag attttgtaaa cggttcacaa
    31981 gttttgcaag gcgctgaatt agattttgga acatccactg ttaaaagtac gtcagataac
    32041 gaatccactg gaccaactag tcaaactgta actgggttga gtaaagcaag tgcagcgaca
    32101 ccaatattta cagcagcaaa agatcaaggt ctgggaacat ggctttctgt ttatgatcca
    32161 gcaaatatca cactgaaagt gccgaaagca gcagctggaa cattcacggc tgatttgact
    32221 tggaacctcg tggctggccc agtagcatag agtatgaaat aaattcaaca aactgcggtg
    32281 aaaatactat aggtgaagga ttttttaccg cagttgttgt tgaaaggatg tggtggacaa
    32341 tgaatcaaag taaacgactg tttgtttcgt ttttttctat tttaagtcta tttttcattt
    32401 ttccgtcaat cagccaagca aaggattcag caggagactt tggaatcaaa ccggtatttc
    32461 cagagaacca aatagataaa gcaatcggtt attttgattt attagtggca ccggaacaaa
    32521 atcagacact agaagtaatt atcagcaatt cttcagatga agaacgaaca tttgaggtct
    32581 ctgtcaatcc ggcggtaacg agtgatggtg gaacgatcga ttatagtcaa aaaaacccaa
    32641 cgttagatga gacattgccg tttgatgtcc gtgacgtttt gctcattgcg aaaaaggaaa
    32701 tcaatgtatc agcacatgca gaaacaacta tacctataga agtgaaaatc ccagcaaaaa
    32761 gctttaaggg acgtgtatta gcgggtattc atgtttcacc taaagaagaa gctgaaacag
    32821 aaaatgctac agaaggagca caaatcaaaa atcgtattgc ttataatctc gcagtcgttt
    32881 tacaagaaag tcaagagacg atagaaccgg atctgaaact tttatcagga gatttggacg
    32941 aggtcaacgc aaagccaact gttcagcttc gtttccaaaa tcctcagcct aggattatca
    33001 gcaatttgat atttacgagc aagatctttt atgaaaatca gctttatatt gaaaatacgt
    33061 ctaatgaatt tttagttgca ccaaattcga attttcactt gaatcttgat ttagctggtg
    33121 ataaagcaaa agccggcgat taccgcgcag aaatcatagc aaaatcaggc gacagtaatg
    33181 aatggcgatt tacacaaaac tttacgatca aaaaagaaaa agcacaaaaa gtaaatgaaa
    33241 attccgtttt tgcagtacaa gaacaatctt tcccatggct ttatgcagga ttggttgctg
    33301 cagttctagt gttatggttg attttgctgt ttttgtgggt ccacaagaaa aagaaaaaag
    33361 gagaaacaac atgatgaggt gatttcgttg aaaacatcga tcttattggg agggattatt
    33421 gcaagtttgc tgttcttcag ctggcaagag ccagtccaag cagataccaa aagttcaggt
    33481 acgattacat tttatgatct aagtccagtt ccgcatccaa gtggttcagt caatggcgca
    33541 tcaaaacagc agacacagct accgatttat aaagaatcta atcaacatgg cgtattcccg
    33601 gagacgaatg aagaaaagat ccgttttctt tatttgatca ttggtataga attatggctt
    33661 cttgcgtggt tgttgttatg gagaagaaag gaaaaggagg atgaacatgt tgctacaaaa
    33721 taaattttca ttttttcgct ccttcttttt tacttcgctt tttgttttaa gtatatttat
    33781 cttttcacct tcggcagaag ctgttggagc aacgcctcct gccatttctt atgatccata
    33841 cacgtatgaa aatatcgtac ctccaaatgg tggacgtagt gcagtcaatg gcgggaagct
    33901 tgtccaacta actggggcta accaattgcc taaattgact gctacttatt tgaacgatgg
    33961 ttcaaatgat ccttggaaag gtggcacggt ccgttttcat tgggaaagcc atgtgaaact
    34021 aacaaatgta cttgatttgg tgaatatcac tgcacaagga tttgcatttg tggcgatcat
    34081 caatttgccg gaacatgtat caagcagtga tgtattaaaa gcaattgact ggaacaacgc
    34141 ctacttgtat attgccggtg cccccatcaa ggttactagc ggtagtttat tatcagcagg
    34201 aagccatacc ttgcgttttg ggatggggtc gtgggataca aatactctat tggcagatat
    34261 tattgctatt attacgcgag gcgaattcaa cgtggacgat atcccgcttc aatttgatgt
    34321 tacagctaat gtcgcgaaga tgacagaaaa cggagataaa tatgatacga gtccaaacaa
    34381 aattttgacc aagggtaaat tacaaccttc tatgtctaaa aaggccgttt tttctgttga
    34441 tttttatgat tcgaataata ttatccaaga tcgtatgttc aattcgattt tagctccagc
    34501 tgcaggtaga ttttatccta aattaactgg cggaaacttg aatccaggag catatgcatt
    34561 ttctgcaagt gcagagataa atacgtggaa tagttacatc tcaacaagtg attcaacagg
    34621 aaaatataat acatttgctg ataccacaga atacgtggat ggaaataacc agaaccttcc
    34681 aggctctacc aatagtcgga cattgacgat ggggctgact gaaggacgtt ttgatcagtt
    34741 gccatttagt cgctttgatc gcgtagtcaa ctattttaca ggaaaaaacg ttacacaagg
    34801 agcaatactg actcatactc cagcaagcac aagtggattg aatcagacag ccaatatcgt
    34861 gtacagtggg aaagatgcgc aaggcggatt gctttctcct gttgctttaa aggtattgga
    34921 gcagtacaag ctagacggaa cggttttacc aacaaactta acagccgata gtcagtgggg
    34981 aaagctcctt tcttctgcat cgtaccaagt cacggccaat tggtcagcaa ctgaactaaa
    35041 aaagggttcg atccattaca ggctttattc gaaaggaacc caaaaattag cgacaggatg
    35101 gacagatcaa cttttccaaa cgatccaatc aaataacggg ggtgtaaata cagctacttc
    35161 tacactacct gcattggctt cagggcaata ttattttgat tatcgcttgg tggacgatgt
    35221 acttactaac atgtatccgt cttttgcgta caaatggcag tctgaacaag ctggaatcac
    35281 cactcttcct caaataacag tggctaattt tccatctgtg agtgcagata gtcagctaaa
    35341 aaaccttagc agacaacctg agacaggaga tcccctgtct gctctatcag gagatacgat
    35401 tcaagaaaat gtgacattca cgttaacaaa atctggggat gcgataaccg acaaaaaaat
    35461 cacgatctca cttcctgaaa atactactta tatcaatggt tccttaaaat taaatggtac
    35521 aactatcgca gataccggca ttcaacaagg agtttctgtc ccagcagatc tattgagcaa
    35581 aataggagat accattcatc ttacttacaa ctatcaatta aatactgtag atacttctgt
    35641 acaatccgtc agcatactga caaaggcagc tgtactttcc agcaatatta cattagcaga
    35701 tggtgctaag cttcctaacc cagttgtcca aacatctgct aaaacaattc ttgtccccaa
    35761 acaagaatta acattagtta atgtaccaga tgattttaca tttggtaacg atctccccaa
    35821 accgttgaag acaagttatt atgaagcaaa aggcgatttt tcttttgatg tgcgggatac
    35881 tcggcttcca agcacgtctc cttggcaact gacagggaca ttgacttctt tatttaagaa
    35941 tgatcaaggt caagaattaa gcggaacaaa attatacttc aatcattctg gatcaaaaca
    36001 attgattcag caaggtcaaa acacgttgat ttatgagagt gatggaacag ctaaaggaga
    36061 ggttctcgtt gactttcccg ataccgatgg tttactctta gaggtaaata gcagtacgaa
    36121 tgctcaacct ggcgcaacgt atcaaggcat ggtcacatgg gaactgacag ctgggccaac
    36181 cagctaagtg attagaattt gtatggatct gtgataactg tcacagatcc tctttttcaa
    36241 ggatgagaag gaaacttctg ctgttaaaat cagaaatgct tggaggagtg gacttggaac
    36301 ttttcagttt gcttgaaaaa aatgaacaac ggcaaatcaa acttttccaa cttttattag
    36361 aggaaaaagg agagatttcc gttaataacg tacttacccg cttatcagtt gatcgggcta
    36421 ccttgaaaga agatctgttt tatttacaag ctaacttgga aaatatcaaa caattggaaa
    36481 ttgttatcca agaagggaat attttattaa tcagacatgg aaatatagca gcagtcgatg
    36541 tttattatca ttgctacttg cgctattcta caaaatatca gatacttctt tatctgttgg
    36601 aaaatggctc atttgatcgg cagaaacttg tcatggaact taatatcagt tcggctactt
    36661 tgtctcgcag agtgaaggaa ttaaatgatt ctttaaatga gtttcatctg aaaataaaaa
    36721 gcgggcggct gaccggtcca gaaagtcaaa tttgttattt ttatttccaa ttattttggt
    36781 ttggacgtcc ttatcttgaa aacaaaaaag aatacagtga tttatcagga aacacttttt
    36841 tagatctgct gcaaaaagag tttagtttta ctattacaga agaaggacga ttgaaatttg
    36901 aaatatggct ttccattatg aagaagcgaa tgcgctgtac gaaaaaatcc aaaccagatt
    36961 atagcaatct tcctgttgga ttgtttcaaa cgacacaatt tttattggac ctccatacat
    37021 ttttgacccg ttacttcttt caatatgcct ttccttggaa tcaaaatgaa acgttggttt
    37081 tttatctttt catgatcagt aactttacat tagatacaca taactcgcat gtccaggagt
    37141 ttttgataag cgaaaaatac aaagacgacg tagtagctca attgaatcga acgtttaaac
    37201 aatcgataga agaatatttc tctttttatg tttttccaga agagatttcg caaaagattg
    37261 cgttgacagt gatgcaaaat cacgctcatt ttgcctattt tagaggatgg atgaatattt
    37321 ttggccaaga aggattaaaa cagcggttaa ctgaagtaga agataaacaa ttcctggttt
    37381 tatgtgaaag attggtcaga caaacatttg ctattgttga ttttactgaa gtagacgagg
    37441 aaaatgcccg ctttgaatta tttgctcgct atgcatctat tttgcggacg ctgatcaagc
    37501 agttagaatt acgtctgcaa attgcttatg atcttcctta tgaaaggcta atggcggata
    37561 tcgtgaaaga aatcatccag gaacaattgc tgaatacgat caaatatgat ttagtcgaat
    37621 acgagaaaga aaagcagtat gatatcattt tgactagcca attgaaagag tacccgtcac
    37681 agaagtccgc taaagttttt gtattcacta gcaacgaata taattatgat tgcccctatt
    37741 tgaaccaatt tttaaaagaa tgttatttag aaaaattgaa tttaaggatg aataaaacat
    37801 gaaaatcgtg atcattggtg cttcccatgc aggcatcaca gctgccctaa atctaagaaa
    37861 acttcaaccg aaagcagaag ttttactgat cgatgaagat cataaagatg gactaggcta
    37921 tgtttcaaat ggaatcaacc tttatttaaa aggaaagata cgtagtttag ctgaagttgc
    37981 ccataacatg cggactcttc aagaaagtgg ggcaaaactg atcaccgaat ggagagtaac
    38041 tgaacttgat ccagacaaac atcagctcat cctcgcatcc aaagaaggaa aaaacgagac
    38101 cataacctat gataaattaa tcgtagcaac aggttcctct cctgtcacct tgtataaaca
    38161 aattgaagct gaaaatgtct acacctataa aaatctagtg caatctaagc aagtacttgc
    38221 cgctttaaaa gaagcaaaag aagtagtgat cttcggtgct ggttatattg gattggaatt
    38281 agcagatgct ttgagaaata aaggatacat tattcatcta gttgattata tgccgaatgt
    38341 tttatcaaga tatttcgata aggatatgat caacagtttt caaaatcagc tgcaaactaa
    38401 gcaaatcaat ttttatccaa atgaattctt gatcgattgg aaaaaatcag aagagaaagt
    38461 agtatccgta caacttctat ctcaagcaat aaaggcagat atggtcattt tttctgccca
    38521 aactcgtcct aatacgacat tgttaaaaga aaaagtggct ttgtatgagg atgagactgt
    38581 gatagtcaat gaatatctgc agacaagtga tccggacatc tatgcgattg gagatatcgt
    38641 cccagtcagt tttgacaaaa ataaacggca tctcttttta cctttagtta ctcgagctgt
    38701 acatatggca agagcagtag cgctaacttt gagtggtcag ccaaccgctt acgacctcag
    38761 acaaaagata acagcagcag tcattataga ctacttttta ggaactgtag ggctgacaga
    38821 agatgaagcg ccattcttgg aacaaaatac cggttcgtgt tcaggagaat ttgatctatt
    38881 tccacaatac gatgaagaaa acaaaacagt aaatgcaaag ctgatctacc atccggatac
    38941 attagaaata attggcggac agctgataag ccaagaattc ttattatcag atttgaacct
    39001 gcttgcggat atcgtgaaac acaaaactac gattccacag ctagcagttg aggattttgg
    39061 atttctgtct gaacacacct ctcgttttca ctacctcaat gagttagctt ttaaagtact
    39121 agctaagaga gcaaatagca atagagtaga taaaagactg tgagaaatat cacggtcttt
    39181 tattgtgtgg ggatataata aatattaatc aatcgagaat aaaaaattca tataatgaaa
    39241 tagcggaaga attttttgag cataatattc gccaaaaatt tatcaagtga taaaattgtt
    39301 gtataaaatg attatttcag gaggaggagt taaatgtttt tagcattaaa tgaaattatg
    39361 cattcaaagt tacgttatgc gttagttgca ggggtgatgt ttttgatcgc ctacctggta
    39421 ttttttttga ctgggttagc ttatggctta gctcaagata atcgaacagc agtcgacaag
    39481 tgggaggcag attcgattgt gctttcgaaa gatgcaaatt caaatctagg gatgtccatg
    39541 attactaaga agatagcaga agaagtcgaa ggtgggaaag tggcgtatct tgcccagaca
    39601 cctggagtcg tgactagtaa agacagtaca gaagaaggaa aaatcaatgt tagcttcttc
    39661 ggtatcgata aaaatcaatt catcatgccc aatcttgttg aaggaaaagc ttttgataac
    39721 gatgatgagg cagtaggcga tattagtttg aaagaagaat atggtctagc cgtaggagat
    39781 acagttaagt tatctggtag tgacaagacg tttaaactaa ctggctttac ggatcatgcc
    39841 aaattcaatg tctcaccagt tctctataca accatcaatg cttaccaaga gatccgcttc
    39901 gaaaaagaag atacaagtga aaatgcacgg attaacgcaa tagttgtcag aggtaaaatc
    39961 agtaatttac ctgaagattt agaacagatc aaaatttcta aattcatcaa tgaattacct
    40021 ggatataatg cgcaagtatt gacttttgga tttatgatcg gatttttgat cgttattgcg
    40081 gctattgtga tcgggatttt catttatgta ctgacgatgc aaaagattaa tatctttggt
    40141 gtaatgaaag cacaagggat cacaggagga tttattgcgc gttctgtagt ggcacaaaca
    40201 ttcattttat catttgttgg tattcttctt ggtctattag gaacagtggg aacatccctt
    40261 gttctacctg atgcggttcc attccaaagc aattggctgt tttttggcgt cattagcttg
    40321 ttgatgctgg tagtagctgt gttaggcgca ctattttctg ttcggacaat cgtaaaaatc
    40381 gatccgttga aggcaattgg ataagaagga ggaagagcat gaaagcaata gaatttaaag
    40441 aagtagaaaa aagtttccaa gatggcgacc aagtagttaa agcattgaaa gaaacaacca
    40501 tgagtatcga taaaggggaa tttgttgcaa tcatcggtcc ttctggttca ggtaaaagta
    40561 cttttttgac tttggcaggc ggattgcaga cgcctagcag tggctctgta tttatcaatg
    40621 atcaagaatt cagtaatcaa aaagaaagtg aacgcgttaa actacgtttt aaagaaattg
    40681 gctttatttt gcaagcttcc aatctggttc cttttctgac agtaaaaaag cagttattat
    40741 tagtagataa gatcaaacat gaacatcgtc aaaaagaagc aaaagaatta tttgagcagc
    40801 ttggcgtgga taagttgatc aacaagtatc cagaagaact atctggtgga gaacggcaaa
    40861 gggtcgcaat cgctcgtgca ttgtacaatg atccgtccat tattctagca gatgaaccaa
    40921 ctgctagttt ggattcagaa aaggcacgag aagtagtcga tatcttggca aaagaatcaa
    40981 aagacaaaaa taaagcaacg atcatggtta ctcacgatac acggctgatt gacaaatgcg
    41041 atagaatatt tatgatggaa gatggtgtat tgaaagaaaa agaataacca aaaagcaggt
    41101 ggaaaaatgg tccatcgata caacaaagat acacagcaaa aaattttgaa agaagcgact
    41161 gacttgttta tgtccaaggg atatctcggc acttcaacaa gagagatcgc acaaaaagtt
    41221 gggatcaccc aacccaatct ctatcattat ttcggtgata aagaaaaact atatacggca
    41281 gttttagaaa accacttaaa agatgttgga aaagctttga gagaaatcgt tcaaacaagc
    41341 gaaacgggat ttcaagtaac cttgacaaaa atggcgcagt ttttgattga gacacatctg
    41401 gtcgatttgt ttctgatgct tcacgatctt gaaagcaatc tttcaaagga aacaagagat
    41461 catcttttct ttctatggaa gaaaaattat cgtgaacctt ttgaagaaat cttttcagca
    41521 aaccaatctg tgctaagaaa tgggatttca aaagaaatcg cagcaaggca ttttttctta
    41581 gtgttagcac cttacatcac tcggtcgaaa agacctgtgg aagaaccgct aaatgtggcg
    41641 caattgatcg atttatattt gaatggtgta ttaggagaag cgtaaagctg ttatataggg
    41701 agaaaaatag ttaggtgaag aagcaagata tgacgaagat cgttatatct tgtttttttt
    41761 atataggtag tttttcataa atacaaattt gttattctct catgattatc agggtatatt
    41821 tttatttatt atgtcggtgt tccaggaagt gttttaattt aataacgaag aaattaattg
    41881 tttgtagaat gaaggaaact atttattggg tagatgaatc accacgaata gtcaaaagga
    41941 gatttcagga tatgccaaaa atgaaaaaaa tcattttagc agtaatgtta ttttctacgg
    42001 ttatatcttt tacagcccct tttttgcgta cttaagctga ttttaagttt tgacccttag
    42061 tctggatcta cgaggtgaga gtcaagaatg aaaaaaataa ttggagcttt tgtatttgca
    42121 atcattatca ttattatctg ttttcaaata gatgtttttg gtcaaaaaaa gatagttgga
    42181 caaacggtca gcagtacgaa cagtacggtt acaaagacaa aggaatcatt tgatgataca
    42241 gaacaagaga cattaatcaa aatcggaaaa aatgaccgta cactatatac agatgactcg
    42301 ctaactaccc cacttgcaac tataaacggc ggagagctgg cggagtttct ttctgaaaca
    42361 aaaaatgctt atcagatcaa gacaaatgat ggctatactg gatatttagc cctcgaagat
    42421 ggaaccaaag tcacaaagaa tatacaaaca aaaccggaag agctatctga tgcagtgatt
    42481 gtattagatc ctgggcatgg cggaaatgat actggggcat taagcaatga tgaacaaaca
    42541 gaagaaaaag atataacatt aagtacagcc ataaaagtaa aaaaggcgct agaggatgct
    42601 ggagcaacgg tttatttgac ccatactaca gatgaattag tacagcttgg agatatatgc
    42661 gactacagtg aagaaaaaaa ggcagatgtc tttatcagtt tacacgcaga ctctacggaa
    42721 tatgcgaatg aagcaactgg aatcacgaca tattattact atggacaaga ggaaacatta
    42781 gcacagacaa ttgctgactc cttcactgat ctgcctctgg attcaagagg aatctccact
    42841 gggaactatc aagttcttcg agaaaatctt caaccttcta ttttaatcga gatgggttat
    42901 atgaataatg atagtgattt aaaggaatta gtaacagaca gttatcagca gcagattgct
    42961 gcaagtttga cacaagcatt gaccacttat tttcagtaat aaataagccc agccaaacaa
    43021 aaatagctcc tcatattttt cgtttggccg ggcttattat cgcagatgca agcttttgaa
    43081 caccgtttaa ggaagaggtt gtgagcctgc cgtttaactc cgcgtcacaa ggagcaattt
    43141 caaaaaacag cttctcatgt ttgatgacta ttgtgacttg tgaccgagga gttggcatga
    43201 gaacaccgtt tattcggaac aagggactgt gacatgactt ttgtcacagt tccattattt
    43261 ttttagcagc ttcaaagctt tttctgtatt ggcaaaatga agtttggcat atttttcaag
    43321 cataggcatg ccgcctttct ccaatagttt tgccgctact tggtcagatt tcgttccagc
    43381 tcctgatgga agggtgattc cagcttcagc tgatgctttg tgtaactctt ccaaaaagct
    43441 ggcacgactg atgatagaag cagcagcaac agctaaatga tattgttctc ctttagtaac
    43501 gaagtacagt ttttcttgta attgattttt ttctaaacgc gcatattttt taaaatttgc
    43561 ttccggcgta aactgatcga tcaaaatcgc ttctggtttt tctggctcga ttttttttag
    43621 cagcaaatgg atcgcttgat tgtgcaaagc gactttcatc cgaacggcat tgtattttgg
    43681 ttggatctca ttgtattttt ctggagataa gacaagcagt ttatagggga tggttttttt
    43741 taagatgtca gcaagctgga tgatttggct atcttttaac tcttttgaat ctctaacacc
    43801 aagtttacgt aattctgcaa gctgatcacg tttcacataa gcagcacata ctgttagcgg
    43861 accaaaatag ctgccatttc cgacctcatc actacctaaa acggataatt gactgatatt
    43921 ttttggcaaa gaagaggctg tttttgctgg cttggcttgt ttatcctgtg cctcccccca
    43981 tttatcagct tctattgctg cattcgtacc ttgaaacatc acttttccgg atgtataagc
    44041 ggtaatggtc gtcccgtctt tttttgcagc aaagctagta taaggaatct gtttatttaa
    44101 caaataagag cgatagtatg ctttcatttg actaagttgg tcgtttgttg ctttgataat
    44161 gacgttgctc ataaaattct cctcgttttt atacataaaa ctataccgca actcacgact
    44221 tttcgttttc taagagattg cgagacatag cattttttta aagtttatca tataatagtt
    44281 aagtatgtga aaaatatttg tggagaacgg tgaaagaacc aacttcatgg tacactaaat
    44341 aaaactttca tcaaatagga ggaaatgatt cgtatggcgc acgaaaaaac acgctacaaa
    44401 gcagtgattg ccaaccaaac atatactatt atcggacggg aaaccaagca tcatatggat
    44461 atcgtaacga agctgatcaa tgaacagtta gcagaattga aacaattatc accacaaatg
    44521 gataatgaac aagcagcaat cttgatggct gtcaatgcgc tttcggatca attgaagaaa
    44581 caagaaagaa tcttggagct ggaagaagaa acagcagaat taaagaaaaa aatgatcaaa
    44641 tttacagagc ttgaaaatcg tgtgaaacgc attgaagcaa tcgagaacga agctcgggaa
    44701 gtattgaaag aaaatggtca agcagatcat accattaaaa atcatgtgga agcacaacag
    44761 attttaaacg agaaacgaaa aggacaaatt aaacaaaaat cttctaatta agatcctttt
    44821 atcaaaataa gaaaaattta aaaaagttag aagagaaagg aacgtagcat gctgagtttg
    44881 ctgattttat ttttattgct aattgcgttt ttttctggag cacgtcgcgg gtttgctttg
    44941 caaatcatat tcgcgattgg atatgtattg tcatttattg ctgcccagca tttttacaaa
    45001 ccattagcaa gtcatttgga attatacatt ccatacccag ctgtcacccc aacatcaaag
    45061 ttagcatttt ttgatcaggt atttgctttt cacctagatg aagcttttta tgcaggaact
    45121 gcttttctga ttattttgct aattggctgg ctgcttaccc gatttgtggg ggtttttgtc
    45181 catggattga cgtatgtgcc gatcttgaga caggtagact ggattgcagg aggcatcctg
    45241 tcactaatca tggcttacgt tactatattt ttgattttgc agttacttgc ctttgttcca
    45301 ttagatgtgg tccaaaatca attcggcgag catagtttcg ctcgttttat cgttgagaga
    45361 acgccatttc ttaccaacaa gatgtatgag ctttggtaac gaatattttg aattaaggaa
    45421 aatagtgctg gaaaatattt cgttttttgt acagagtagt ttatttaaga aggctgcgta
    45481 gcgtatgtcc ggcacagctc ttttttcata gtgaaagata aggtgataaa atgaataacc
    45541 gaatccttga aacattggaa tttgaaaaag tcaaacagat ggtcagacaa tttgtagtga
    45601 ctgcacaagg aaaagaagaa ctggcagaat tggtaccagt gagtgagaaa caaacgatca
    45661 caaactggct tcaagaaact gaggatggat tgaaagtgca acgtctgcga ggcgggatcc
    45721 ctatacctaa acttgaaaat atccgtcccc atatgaaacg gatcgagatc ggagcagact
    45781 tgaatggagt ggagcttgca caagtttcca gagtattgtc tacgacaagc gaattgaatc
    45841 gtttcattga tgatctttct gacagtgaaa tcgaatttgc acgtttgtat atgtgggcag
    45901 atcagttagt cacgatccct gttttgagcc gccgtttaaa ggaagcaatc gatgaagacg
    45961 gacgcgtgac tgatgacgct tctcctgagt taaagagcat aagacagaac attcggcgaa
    46021 gtgaacaagc tgttcgtgaa cagttagatg ggatcgtccg tgggaaaaat gcaaagtatt
    46081 taagtgatgc gatcatcacc atgcgaaatg accgctacgt tatcccagtc aaacaagaat
    46141 accgcggtgt atttggcggt gtagtccacg atcaaagtgc ttcaggacag actttattta
    46201 ttgaaccaaa acaagtggtg gatttgaata atcgtttgcg tcaataccaa atcgctgaac
    46261 gaaatgagat tcaacgtatt ttaagtgaac tttctgctga acttgttccc catcgtcaag
    46321 aaatcatcca caatgcttat gtgattggta aaatggatct gatgaatgca aaagcacgat
    46381 ttggtaaaga agtaaaagcc atcgtgccgg gaatcagtga agacaatcat gtcgtcttga
    46441 aacaagcaag acatccactg atcgatcagg aaaaagtagt ttctaatgac atcacaatcg
    46501 gaaaagacta tcaagccatc gtcattacag gacctaacac aggtgggaaa acgatcacgt
    46561 tgaagacttt aggtctgtta caactgatgg ggcaagcagg attaccgatt cctgcaggtg
    46621 aagaaagtca aatcggtatt tttgaggaag tatttgcgga tataggagac gaacagtcga
    46681 ttgagcaaag tctatcgacc ttttcttctc atatgacgaa cactgtagat attttatcaa
    46741 aagtcaatga aaaaagtctg gtattatttg acgagctcgg tgcaggaaca gatccgcaag
    46801 aaggtgctgc tctagcaatc gcaatcttag atgatcttgg taaaaagtct gcttatgtga
    46861 tggcaacaac gcattatcct gaattgaaag tttatggata caatcgggca aatacgatta
    46921 atgccagcat ggagttcgat gtggatacac taagtccaac gtatcgtttg ctgattggcg
    46981 ttcctggacg aagcaatgcg tttgaaatct ctagccgatt aggtcttgat acagaagtta
    47041 ttgatgaagc gaagcagttg atgaatgatg agagccaaga tttaaacgaa atgatcacag
    47101 atttagagaa tcgccggaaa atggcagaga ccgaatatct ggaaatgcgt cattttgttt
    47161 ctgaggcaca agaacttcac gatgatttga aagaagctta cagctatttc tttgaagaac
    47221 gtgaaaaaga aatggaaaaa gcaaagaaaa aagcaaacga agtcgtgtca gaggcggaag
    47281 aaaaagcaga aaaaatcatt gctgatattc gtaaaatgca acagcagatt gggcaaggaa
    47341 atgtgaaaga gcatcagttg atcgatgcaa agacccaatt agcaaatcta catcaagaag
    47401 aaactttgaa gaaaaataaa gtattgaaga aggcgaaaga acaaaaaacg ttgaaaccgg
    47461 gagacgaagt gttggttacg acttacggcc aaagagggac gcttttacgt aaaaacggga
    47521 atcagtggca agtagaaatc ggtatattaa aaatgaatgt gtccgaagac gagctgacgc
    47581 ctgttgctcc tcaaaaagag ccgacacaac gagttatcca tgctgtacga tctgaatcta
    47641 gtagtcacgt gccgaatcaa ttggaccttc gaggaaaacg atacgaagaa gcgctaagtg
    47701 aggtagatca atatttggat tctgccattc tagctggtta tcctcaagtg actattgtcc
    47761 acggaaaagg aacaggcgca cttcgcaagg gaattactga ttatcttaaa aaccatcgga
    47821 gcgtcaaaag ctttgagttt gcaccggcaa accaaggcgg aaatggtgcg acaattgtta
    47881 aatttaagta aagcttacta aaggtaagca gatggttcgc tatccaagta tagaaatgct
    47941 atactaatca tagattttaa aaatggaggt ctttgatatg tcacaagtaa ttacagataa
    48001 gacatttaat gaagaaacag ataaaggact tgtattgatt gatttttggg caacttggtg
    48061 tggtccatgt cgtatgcaag caccgatttt ggatcagttg gaacaagaat atgatgaaga
    48121 agaattcaga attgctaaaa tggatgtgga tgaaaatcca gaaactcctc aacaattcgg
    48181 tatcatgagc attccaacat taatgttgaa aaaagatggt caagtagtag aaaaagctgt
    48241 tggtgttcat tcaaaagaac aactacgtca aatgatcgat caatatttat aaaaacttac
    48301 agcaaaaata agctgagggt gggactgaat ccagttccgt cctcggtttt tttgcggttg
    48361 agttaaggtt attcaaatta atattttttg agtataatta cgttgtcagg ctggattgtt
    48421 tttttgacag acatgctata ataactttaa ttgaatatga aggggagtac cagatgaacg
    48481 aacgaatcaa aaacaaatta gctttacttc ctgatcagcc cggctgttac ttgatgaaag
    48541 ataaaaatgg aacgatcatt tatgtcggca aagccaaaat attaaaaaat cgtgtccgct
    48601 catattttac tggcagccat aatacgaaaa cggaacggct tgttagtgaa atcgaagact
    48661 ttgaatacat cgttacagaa tcgaatatcg aagcattgct cttagagatc aatctgataa
    48721 agaaaaatga tcctaaatac aacattatgt taaaggatga taaaacctat cctttcctta
    48781 agataacgaa tgaaaaatat ccccgtttgg tgattactcg taaagtacta aaagacaaag
    48841 cactgtattt tggaccatat cccgatgtcg gtgcggcgaa tgagacaaag aagatcctcg
    48901 atcgattatt tcctctgaga aaatgcaaac ctagtcagac gaaggagcct tgtctttact
    48961 atcatttggg acaatgtctg tgtccttatt atttcgacgt agatccgaaa gtctatacga
    49021 aaatcgttga tgaagtgaaa aagtttttaa acggaggaca cgaaaaaatc gagtcagaga
    49081 tccatcagaa aatggaaaaa gctgctgaaa acatggagtt tgaaaaagct gcagaatacc
    49141 gagaccagat ccgtgccatt gagacaatca tgactcgcca aaaaatgaca aatactgatt
    49201 tactggatcg agatgtattt ggttatgcga ttgacaaagg atggatgtgt gtccaagtgt
    49261 ttttcgtaag acaaggaaag ctaattgaac gagatgtttc aatgtttcct ttctataacg
    49321 aagcagaaga agatttcttg acttacctcg gtcagtttta tcaggaaaat gagcatttca
    49381 ttcctaaaga agtcttgatt cctgatacga tcgacaagcc cagtgtagaa gctttgttag
    49441 ctacgaaagt gctgcaacca aaacgaggag aaaagaaaaa attagttgaa ttggcgaata
    49501 agaatgcctc agttgctttg aatgaacggt ttgatctgat tgccagaaaa caggaacgga
    49561 cgatcggagc agttgaacgt ttaggaaacg cgatgaatat cccgacccca atacgtatcg
    49621 agtcttttga taactctaat atcatgggga cgaatcctgt ggctgcgatg gtcgtattca
    49681 ttgatggaaa accggcaaga aaagaatatc ggaaatataa aataaaaaca gtcaacggac
    49741 cggatgatta tgcatcaatg agagaagtca tctatcgcag atattcacga gtgattcgcg
    49801 aagggttgcc tttacctgat ttgatcttga tcgatggagg aaaaggacaa gtagatgtgg
    49861 caaaagatgt cttagaaaat caattaggaa tcgatattcc tattgctggc atggcaaaaa
    49921 acgataaaca taaaacaaat gagttgctat tcggtataga tttggctgta gtaccgcttg
    49981 agcgaaattc accagagttt ttcttactcc agcgaatcca agatgaagtc catcgattcg
    50041 caatcacttt ccatcggcag ctacgcagta aaaacagttt tgcttcccga ttagatgaaa
    50101 ttgaagggtt aggaccaaaa cgaaaaaaaa tgctgcttaa agagttcaaa tcgttgaaaa
    50161 atatcacagc agccacgaca gaagaattac aagaaatcgg actgccgaaa aatgtcgcac
    50221 aaaacgtatt tgataaatta catcaggaat gaggaaaggg cactttacaa agaaagtaaa
    50281 gtgtcctatc attttatgta caacaaaaag agcgtttacg ttttgttggt aaataataaa
    50341 tcgcttacgt ctggtgtcta aggatatatg tgatttcttg aagaaggagt gaagaaatgt
    50401 tagaagtcaa ggatcttgtt aaaacttttg ggaactacac agctgtcgat catgtctctt
    50461 tccaaatccc cgatggaaaa atcatggggc taattggcca aaacggggct gggaagacaa
    50521 cgacttttcg tttgatcttg gattttttga cacaagacca aggagaagtg ttatggaatg
    50581 gccatcatct tggggagaaa gattatgatg tcatcggtta tcttccggag gaacggggac
    50641 tttatccgaa agtaacgatt caagaccaac tgctttattt tgcagaactt agagggaaat
    50701 caagaaaaga gattgaacca aaaatcgatt tttggatgga aaaatttcag gtaaaaggga
    50761 aaaagacgga taaagtaaaa tctttgtcta aaggaaatca gcaaaaagtc cagctgatcg
    50821 ccacgttaat ccatgaacct aaattgatca ttttggatga gccattcagc gggttagatc
    50881 cggtaaatgc tgaattgcta aaagatggaa tattggaatt gaaaaaacat ggttcatgtg
    50941 tcattttttc tagccacaat atggataatg tagaaaaaat ctgcgatcat ttgatcatgt
    51001 tgcgaaacgg aaaaatggtt ttgaatggaa aagtacatga gattcgtgaa tcatttggcc
    51061 gtacgaagtt attcttggaa tctggtttaa gccaacagga agtagaagaa atcgaaggcg
    51121 tgaaaaaagt gatccttcat gaagacaata cgttagagat taccttagaa gatcctgaag
    51181 ctgggaaaga aatctttact cgtgcgactc agttcggcta cattcctatg tttaaccagc
    51241 aaccgccaac tttggaagag attttcaaat tgaaagcagg tgaaatcgat gcgtaagttt
    51301 tggattatta ccaaagatgt ctatttaaaa aatgttaagt cgatttcttt cttgatcatg
    51361 atattagtcc cttttatcat gctgggtatc atttatgtag caggaaactt tgctcaacaa
    51421 aatagtgatg tcaataaaat cggcgtattg gctgataatg agcaaatcag tcagcaaatg
    51481 agccaagtca aaacagatga ttttgagttc aaagcagttg attctgaaga aaaagcacaa
    51541 aaagagcttt cggaagaaaa aatcgatgcg tatttagtag tctcaactga gaatgataag
    51601 ataaatggga tgctttatag cgaaaattca ttaggacaaa gtacacaact actgatacaa
    51661 caacaactga ctggcttgca atcgatgctt cgagcaagtc aattaggcat ttcaccagaa
    51721 gaagtagcta gcttgagcca acaagcaagt ttgactcgac aaaaagtcaa ttttgattca
    51781 aaaggaaaaa tgacagttgg agaagataac tcgacagtcc aatatgtcgt aagttatgta
    51841 gcaacgattg tcttgttcat tattattttg acgtatgcgc agatcattgc acaagagatc
    51901 gcttctgaaa aaggcacgcg gatcatggaa gtgatcctgt ccagtacgac tgcacaaaag
    51961 catttttacg gaaaactaac aggtgtttta cttgttgcat taacgcaaat ggcattatac
    52021 ggtattattt ttggtatagg atacagtcaa ttcaaagata tggaaatggt caaaaatata
    52081 ttagacggta tctcattgga taacttgatt ggaccattct tgtggtattc tcttctattc
    52141 atgatctttg gcattttgat tttttctgtt ttagcagctt tatgtggctc tttggtcaac
    52201 aaagcagaag atacagcaaa agcaattttg ccagtgactt atctatctct aggcggatat
    52261 atgcttggat tgatactagg agcttcagat ccaaacaata tcgttattcg gatcacttcg
    52321 tatattccat tcttatcttc atatattatg ccggttcgct tagcaaatga gacagtcggt
    52381 atgggtggag cattgatttc cttgttggta cttgtagccg taacattctt attgatgatc
    52441 ttgtctgcaa acatgtacaa atcgaatgta ctcgtgtata gcgaaggcgg attatggagt
    52501 tcattgaaac aatcgatttc aattatgaga aatgaacgaa aaaaagcata aaatatagaa
    52561 aaatggctct ttgtcaaatg atgttggtaa agagaaatct tagaataatt ggagatcaag
    52621 tggatcagtt cctcttgatc ttttttagtt gaaaatcaat gcgttgccat gacgaatcat
    52681 gatgcgcaat ttgaaattat aaaagctacg aaagccatat gcatttcgtt ttaacacttt
    52741 gatatggttg ttcagacatt caagtgggcc atttgaataa ctttgttgta aagcattttt
    52801 gatttgtggc agaaatttct ggaacgttct caaggtcgtc tgatagcctt cgggaagcat
    52861 gatattattg gtaagtagtt cttcaaacga tacgtagtct cgttccttta cagcgtaaag
    52921 aaagtcttga tataactgat agcctacacg caaagcaggg ctccctttca gtaagcgatc
    52981 cacaatatcg gtctctgtta aatgagcttt gaagccaggt cgccaaaggc gtcgagtata
    53041 atcaagcttg cgttcatctt tttgaagaag tttccagtat ctttttaatt gcttacctaa
    53101 ttgatagtgt ttttgttctt tgctttttaa tagttggttc gtttctttga ttcgatgatt
    53161 gcgaaaggta cgaccaatat gttggacgat gtgaaaccgg tcaatgatca gctgtgcttt
    53221 tggaaacagc tttttaacga gcgatacata aggagcgtac atatcaatca cgatccattc
    53281 gaccgcttca cgaatttctc gtgaaaaatg actaaaataa cgttttaaaa aaggcagttg
    53341 tcggttctcg acaatgtcca acaagcgctg tgtttgtcca tccatcatga tgaaactcat
    53401 agcacctgaa acaccccgaa ctgatttgaa ttcatcaaaa caaagtacct taggtaacgg
    53461 tgtccgaaaa ggattcaacg gacgataaaa tcgcttcata atacggtaaa cagaagagac
    53521 cgatagattt ttctgagcgg cgatctctgt cattgaggta ttttttttca gacgagtcac
    53581 gacagaccag cggactttct cactgatgaa acagtgtttt ttcgcaacag aagtatctgc
    53641 gacaaaggtc cgctgacaag ctttgcattt gaagcgttgc tttttcaatt cgaggtaggt
    53701 acggcattca gaaacatcgt tcattaacaa ccggacggtt gttgtacccc aacgcacaat
    53761 atctgaagca atctggtgtt tacagtggat gcattcttgg gctgtataaa tcaagtttcc
    53821 acgataaata tgacagattt gccctttgta tttttctttt gtgagacaat tttcgttaaa
    53881 agtaactgat aagtctagaa tatctaatgt ttctttgata aggtgagtgt aagacatctt
    53941 gattcctcct tttgggttgg ttcgccaaat taattctaaa ggatttcaag gtgtcttttc
    54001 tatatgtttt ggaaaataaa aaagatgctg gtgtcatcca ccaacatcaa aaagtataga
    54061 gctagaaaaa tatagaagga agctggacat ttattgttcc cagcttcttt tttgtataga
    54121 cctatttcta tttttttatt atattggata tatatattca ttgaaaatag agcataacaa
    54181 ctttataatg ttgagtatac caatttttgg aggcgtttta atggaccgct atttagattt
    54241 actttcagag aagttcccta caacggaaga tgtcatcacg gaaatcatca atctcgaagc
    54301 aattttgcag cttccaaaag gaacagagtt atttttgagc gatatacatg gcgaatttcc
    54361 tgcttttgat catattctgc gtatcggttc aggaaatctt aaagaaaaga tcaaagacct
    54421 tttctctgaa caaatgacgg aaacggagat cagccaattc actattttta cggcttatcc
    54481 agaatatgtg ttgacgactg attggtacaa aaaacaagat aaatcccagc tgatccatcg
    54541 tttgatcgat cttttacggt ttacgacagt caaatacact cgatcaaaag ttcgaaaagg
    54601 attgccgaaa gaatatagct atatcattga ggaactcttg tatatcgatg atcgtatcaa
    54661 cgggaaaaaa gactatgtaa aaaaaatcat tgaacaactt gtcttgatga aggaagaaga
    54721 aaaattttta acaaaacttg cgcagacgat ccaaaaatct gtcatcgatc atttgcatat
    54781 cgtgggtgat atttttgatc gaggaactca agcagccaaa gtaatggatc gaatcatgga
    54841 tttttcctct gtagatatcc agtgggggaa tcatgatatt ttgtggattg gtgcgtattg
    54901 cggctctgag gcatgtttat tgacgctttt acggattgcc gcacgctaca actatttatt
    54961 tgaattagag aaagaatatg gattaaattt acgtccatta ttttcttttg cccatcaaac
    55021 ctatcagaag aatccagtat ttacccctaa aaacaggaaa gatctatctg aaagagagca
    55081 agaagaatta gaaaagatcc atcaagcact ttctatcctc caattcaaat cagaacatca
    55141 gttgttgaat cgacgacctg aatttaaaat ggacgatcgt aaattgttgg aaaaaatcaa
    55201 ctatgaggaa tcaacgatcg ttttagaagg tcagctctat gagttgaaag ggacttgttt
    55261 tcaaacgatc cagccagatg atcctaaaaa attcttggaa gaggaacaaa aagtgatcga
    55321 tagtttgatg tattcattcc agcactcgtt aagaatgcaa aaacacatga cgtttttgat
    55381 cgaaaaaggg ggaatgtatc tcacgaacaa tcagcatgta ctgttccatg gctgtatacc
    55441 tgtagatgaa aaggggcagc tcttagcatt tgaattagac caatcttatc gcggaaaaga
    55501 actgctggga ttttttgaaa aacagataac tgaaagcgca aaagatttga ctgcaaatga
    55561 agatttggcg accgatttga tttggtatgc gtggagcggg ccattttcgc ctttattcgg
    55621 caagagtaaa atggcaactt ttgagcgcta ttttctcaca gagcctcata cgcacgtcga
    55681 aaaaagcaat ccctattatc acttgcgaga tgaagagtgg ttcagtgaaa aaattttggc
    55741 agaattcgat gcaaaagaag aaggttcagc aatcatcaat ggacacacgc cagtaaaagt
    55801 aaaaaaagga gagtcaccga tcaaagcaaa cggaaaagca tttgttattg acggaggtct
    55861 atccaaagct tatcaaaaaa caaccgggat tgctggctac tcattactat gcaattctta
    55921 cggtttccaa atcgtgacac attatccgtt cttgcctatt gaacaacaat ttgcaaaaaa
    55981 atctgatcaa acgaatgtaa aaaaagtgat cgagcagcag ctcccacgca aacttaatcg
    56041 gaatacgaca aaaggaatgg aattacagca acagattact caactccaaa ggctgttgga
    56101 ttatcgaaag gacgattgag aaaaatgcta gccaacattg ctcaatagac taaaaagcga
    56161 ctgcaacaaa aggcgtgtcg cagtctcctt cggaattagc tcaaaaaacg gtaaaatatt
    56221 agaagctatt tttctgttta tttctctaat actcaggtca gggcgctgat ttcacgacct
    56281 cttccttaaa cggtgttctc ctgccaactc ctcggtcaca agtcacaata tccaacaaac
    56341 atgagaagct gttttttgaa attgctcctt gtgactcaga gttgaacggc aggctcacga
    56401 cctaatcaga aaaacgatta aaattcattc acaaagaaat cgaataaacg ctgttcagat
    56461 gaatctacac gatgagaaag ttccggatgc cattggacac caaggatttt gtgcatatta
    56521 tctttacttt ggactgcttc aatgagtcca tcgttggaaa aggcgatggc ttttaacaaa
    56581 ggagaaagat ctttgactgc ctgatgatga taggaattca caaagtatga atcagggaga
    56641 aggtcactta ataaactgtc cgaaacgatt ttgacttcgt gagttgcaaa ctgcggggca
    56701 gtgggctgtt gttcgtgttt tacggaccat tttggatata atgaaagatc ttgataaagt
    56761 gttccctcca acacgacatt caatagttga agtcctcggc agactgcaaa aatcggtttt
    56821 ctttgtttca atgcttccaa gatcaatgcc tgttcgaatt ggtctcgttg aatatttgtt
    56881 tccgtcagtt ttggatgagg gtcttccatg taaagttgcg gactaacatc ctgaccgcct
    56941 gcaagaatga gtttgtcgat tttctcaatt agttgaggcg ctgaatctgg agacatgact
    57001 ggcagaataa gaggtaatcc ttgagcatct aaaactgcat ccacaaatcc ctgcggtgta
    57061 tagcttactt gatttccttg gaaagtatct gtcgctcgaa tcagttgatt cccggcaatg
    57121 ccgatgattg gtttcatttt tattccccct aaagtagact aattgtaaca aggatttaaa
    57181 aaaatttaaa ctatgtaaga cttgccccta tacttatctg ttcttgctgt gttactatct
    57241 gtaaagagaa ttttcgtgtg aaattcatga cgatagttga aaatattgct taatttgtta
    57301 taataaaaag taagcagaaa taaaaaggtt gggcgtaagc ttcaaggatc tagttcttca
    57361 aggggtgaga aggacatttt attgaagggg cctgcctata ctaagttatc tttagaggta
    57421 catactttca atgaaaaaag aaagcatgta cctctttttt aaatcgaaag gaagtgaagc
    57481 aagtgttaga agctttggaa caaatccgaa aagcagaaga agaaaatgag aagcgtgctc
    57541 gaaccctacg gcagcagttg aagacctatg cagaagaaaa agagcaagaa ttggagataa
    57601 tccaaacgaa cagtcggcaa cagctgaaac agttggtaga agaaaaagaa caattcgaaa
    57661 agcaagaatt agaaaaagaa aaaatgaaat tgaaagcaga agcagacaag gtgcaagcaa
    57721 ctttgaaaga ccaatatcaa aagcatcaga gtcaagcaat cggtgcaatc attgaaaggg
    57781 tgaaagaaac ttatggccgt cactaagatg gaaaaagtga ccctcatttc agataaaaga
    57841 aatcaggaga ggattttaca agcagtacaa ggaatccaaa atgttgaaat ccgggatctg
    57901 tatcaaaata gaacaaacaa ccagtgggta gaaaaatatt ttccagatac cacgattatt
    57961 gataaagaag caaaattaag cgctttaggc aatcggctga aagacatcca agaagctatc
    58021 caattcatac aacaccacgg cgatagccgt caaaagaaac tccatttgaa acgtcaagaa
    58081 ctttccttac aagacttgga agcaaattat tccgaagaaa atttcatgaa taaattagaa
    58141 gaagtccttg atttaaaaga aaagtgggaa cagctaactg aaaaacgtga tcgtttagaa
    58201 gaagaggaag aatggcttct tcattggcaa cagctggata ttgctccaag tatgtatgca
    58261 agtaagacga ccatttttga gatggggact gtgagtgcag caaattttga aagtttcaag
    58321 gaagaactaa gtaagcttga tgacgtttat atggaagaaa tagattatac gccaaaacaa
    58381 gtttatcttt cgtatgttgc tttaaaaaaa tcagttgctg ctatagaaga aattgctggg
    58441 aaatacgggt tcaataaaga aacgtatcct tatgaacaaa atcctaaaga aaaattgcaa
    58501 gaaaccaaac agcaattatc cattgttcac gagcagcaaa aaaagataac gacagcgtta
    58561 ggtcagtgca gcggctatat ccaagaattt gaatgggtag aagaagtcac acttgccgtt
    58621 gcagaacggg aaaaagtaaa agaacggttt atccatgcca cttatctgat cgttttgcaa
    58681 ggttgggtag atgcagaaga aaaacagaat ttgatgcatg tgttaaacga aactgtatca
    58741 gaagaagata tatacgttag tttcgaaaca ccaaactcag aagagattca gcaggaagtc
    58801 cctactaaat tgaaaaacca tccgcttatc gaaccttttg aattgctgac tgaaatgtac
    58861 agtctgccaa aatatgaaga agtagatcca acgccttgga tgacaccatt ttatcttgtc
    58921 ttcttcggga tgatggtcgc agatgttggt tacggattgc tgatgctcat tgggacaata
    58981 ctagctcaaa aactatttgt tttaccccgc gggatgaaac gtttcgtaaa attctttgag
    59041 attctctcgg taccatcgat catctgggga ttgatctaca gttcattttt cggacaggca
    59101 ttaccaaaag aactatttgg tatccgcttg cctttcccaa tcctatctac gacggatgac
    59161 gtcaatacga ttttgatttt atccgtgatt ttcggtctga tacagatctt ggttggatta
    59221 ttcgtggcag caaaggaaaa tatcaggagg aaagattact tgagtgctgt cagtgatggt
    59281 tttgcctggc aagggatcct gattgggatc gttattgcat tgattggtgt catgttgata
    59341 aaaaatacag catttgttta tcttggcgcg gggctatcga tcattgctgc tttatgtatc
    59401 ttaatcgtgc cgattatcca atcgccgtct aaagtcaagg gtgctgccaa aggagcgtat
    59461 aatctttacg gattgactgg ttatatcggt gatttagtca gttatacacg tttgatggca
    59521 ctaggtattt caggcggaag tattggcgcg gcattcaata tgctagttgc tttcatgccg
    59581 ccagccgctc gattcagtgt agggatcctt ttgatcatcg ctttacacgc actgaatatg
    59641 tttttgacac tgttaagtgc ctatgtccat ggtgcacgat tgcagtatgt cgaatttttt
    59701 ggaaaatttt atacaggagg cggacgtgcc tttgacccgc tgaaaacggc ggaaaaatac
    59761 gtcaacatca atcataagaa aaaaaacaat aaataattgg aggattttta tacaatgatg
    59821 gattacttaa ttagtcaaaa tggtggaatg gtatttgcag tattagcgat ggcaacagcg
    59881 acgatttttt cagggattgg ttcagctaaa ggtgttggaa tgacaggtga ggcagcagca
    59941 gcgttaacaa caagtcaacc cgaaaaattc ggacaggctt tgattctgca attgcttcca
    60001 ggtacgcaag ggttgtatgg attcgttatc gcctttttga tttttatcaa cttaggaaat
    60061 gatatgtcag ttgtacaagg attgaacttt ctaggcgctt ctctgccgat tgcttttaca
    60121 ggtttgtttt caggtatcgc ccaagggaaa gttgcagcag caggtatcca aattttagcg
    60181 aaaaaacctg aacatgctac aaaaggaatc atttttgctg cgatggttga aacatatgcg
    60241 attttaggtt tcgttatttc cttcctatta gtattgaatg cctaagcata aaggaggaaa
    60301 agcagatgaa cgccattgaa aaaatcatct ctcagatgaa tgaagcagca gagcaagaaa
    60361 gagcggcgtt agagcaagaa gagcgaatga agatcgatca aaactttgaa caaaaacgta
    60421 cgcaaatcga aacagaacat caaaaacaaa aagaaaaaca aatcgaatta cttgaaaaaa
    60481 aataccgtca gcttaggaat cgccaacaag tagaagtacg acaagaaaat ttgaatgcga
    60541 agcaggaatt tttacgacga ttgtttgcag atgcaatagc tgaaatggag aattgggatg
    60601 aatctgaaca aatccagttt atcaaaaatt cattgtattc attacctttg acaggcaaag
    60661 taacattcat tgctggagaa aaatcagcag cttatctttc tcagacattg ctggatgaat
    60721 ggaacaatga acttcccttt atgatggttt taagtgatga aactgttgct gatcaagcgg
    60781 gttttctgat aaacgatcaa ggagttcagt ataattttct tttcagcagc cttgtacaag
    60841 atatccaagg aacgatgagt tttgagattg ccaatcaatt attcgaataa ggagggatca
    60901 gtgtatggaa taccacgaac taaatccctt gattcgcgga agagagcttg aacttttgtc
    60961 caatgagaca tttgaacgga tgattcaaac aaattctgtc gaagcacttg gtgaaatatt
    61021 gaaatccacg atttacagtc catatattta tgacggatat gaatatgact tcgaacagaa
    61081 tcttttaaag gagcagagcc gtctatttcg ctggttaaag gaatcagcac cggagcctga
    61141 aatcgtttgg atctatacga tgcgctatac tttccataat ctgaaggttt tgacaaaagc
    61201 cgagatgaca aatcaaaatc tggaccattt gtatatcgac gatggatttt attcattaga
    61261 aaccttgaag gatgccattc atacgcaagt ttcaaatgaa ctccctgaaa gcattatgga
    61321 cagtatacga gaagtacgtg attattttga agagtctacc attcttcagg gaattgacgt
    61381 gatttatgat cgacagtttt tgacagagca gcgacggctt gccgataagc taggatatca
    61441 agaattgctg acagaagtca ttgcttttat tgacttgacc aatatcacaa cgatggctcg
    61501 aggaatattg cagaaaagaa catatgggtt catgacgacg gtactttcca gctcgggaag
    61561 tatcgaaaaa gaagtatttt tgaactttgt agataaagat atggaagatt acacgaattt
    61621 tctattagag acagattatg ctgattttct gcgccctgtc atccgtgagg gaaatcttga
    61681 tctcgtaaag cttgaacagt tgaaagatga ttatctaagt tcattgtatc aagaagcaca
    61741 aacccaggct ttcggaccac tgccactact agcctttttg aacgcaaaag aggtagaaag
    61801 caaaaacctt cgtttactgg ttatcggcaa aagaagccat ttcgaaccag aaacaatcag
    61861 aaagcgggtg agacaagtct atgacgcata agatcggtgt agttggcgac aaagattctg
    61921 ttttgccatt caaattgttt ggctttgatg ttcgttatgg tacaaccaag caaaccgttc
    61981 gtcaggcaat tgaagaaatg gcaaagaatg agtatggcgt gatttatgtc acagagcaat
    62041 gtgcagcatt gagcccagaa acgatcgaac gttacaagga acaaatgata cctgctatcg
    62101 tattgattcc caatcatcaa ggatcattag ggatagggct ggaagaaatt caaaaaaacg
    62161 tagaaaaagc agtaggtcaa aatatcttat agtatgaaaa ggagatagaa attttgcaaa
    62221 ttggaaaaat cataaaagtc tccggacctc ttgtaatggc tgagaacatg tctgatgcca
    62281 gtatccaaga catgtgtcta gtgggagatc taggagttat cggagaaatt atcgagatgc
    62341 gtggcgatgt ggcgtctatt caagtatatg aagagacttc tggaattggt ccaggcgaac
    62401 cggttcgctc tactggtgaa gctttatctg ttgaattagg accagggatc atttcacaaa
    62461 tgtttgacgg aatccaacgg ccattagata cctttatgga aatcacacaa agtaatttct
    62521 tagggcgcgg agttcaattg cccgcattgg accatgagaa aaaatggtgg ttcgaaccaa
    62581 cagtagaagc cggagaaacg gtgagtgctg gagatgttat cgggatcgta gaagaaacaa
    62641 aagttataaa acataaaatc atggtgccaa atgggataaa aggaacgatc aaaacaatcg
    62701 aaaaaggttc attcactatt gatgaaacag tatgtgtcat tgaaaccgaa aatggtgaca
    62761 aaaatttgac gatgcttcaa aaatggccag taagacgtag tcggccaatc aaagaaaaat
    62821 taaacccgga tgcacccatg atcactggac agcgagtgat tgatacattc ttcccagtga
    62881 caaaaggagg agcagcagct gtacctggtc cttttggtgc agggaaaaca gtcgttcagc
    62941 atcaaattgc aaaatgggcc gatgtggaca tggttgtcta tgtgggatgt ggcgagcgtg
    63001 gaaatgagat gacggatgtt ttaaatgaat tccctgaatt gattgaccct aatacaggag
    63061 aatcgttgat ggaacgtacg gtattgattg ctaatacatc aaacatgcca gtagcagctc
    63121 gagaagcttc tatctatacg ggaatcacaa ttgcggaata tttccgtgac atgggttatg
    63181 acgttgctat catggcagac tccacttcac gatgggcaga agcacttcgt gagatgagcg
    63241 gacgtttgga agaaatgcct ggtgatgaag gttatccagc ttatcttggt tcacggcttg
    63301 cggaatacta tgaacgatct ggaagagtca ttgcattagg atcagaaggt cgcgaaggaa
    63361 gtataaccgc aatcagtgct gtatccccgt ctggagggga tatttcggaa ccagtcacac
    63421 aaaatacatt gcgggtcgta aaagtattct ggggtcttga ttccagttta gcccaaaaaa
    63481 gacattttcc ttcgatcgat tggatacaaa gctattctct ttattcaaca gaagtaggga
    63541 agtatatgga tcatatcctt caagctgact gggcatccat ggtcaccgag ggcatgcgta
    63601 ttttgcagga agaacagcag ctgaatgaaa tcgtgcgttt ggtagggatt gattcccttt
    63661 cagataatga ccgtctgaca ttagaagtgg caaaatccat tcgtgaagat tatttgcagc
    63721 aaaatgcttt tgatgatgtc gatacattca cttcaagaga gaaacaattc aaaatgttga
    63781 agatgatctt gacctttggt gaagaaggac gccgtgcgtt gtcgttaggt gcttatttaa
    63841 gtgagatcat ggacggtacc gtatcgattc gtgaaaggat cggacgaagc aaatatatcc
    63901 cggaaacaga attagaaaaa ctagatcaaa ttattggtga aatcaagcaa acgatgcaaa
    63961 cgatcgtttc agaaggaggc atgagcaatg attaaagaat atcgtacgat cggtgaagtc
    64021 gtcggtccat tgatggcggt tgaaaaagtt gctggtgtca aatatgaaga attaatcgaa
    64081 gtccgtatgc aaaatggcga gattcgtcgt ggacaagtat tggaagtcca agaagataaa
    64141 gcaatggtcc aaatattcga aggaacaagt ggtatcaatt tacgtgactc ttctgtacga
    64201 tttttaggcc atcctttaga actaggtgta tcggaagaca tgatcggtcg agtattcgat
    64261 ggtttgggac gaccgaaaga taacggacca gagatcctgc ctgaaaagca tttggatatt
    64321 aacggagaag taatcaatcc agtagctcgg gattatccag atgagtttat ccaaacaggt
    64381 atctctgcaa tcgatcattt gaatacatta gttcgtgggc aaaaattacc agtattttct
    64441 ggctctggtc ttcctcataa agaattagct gctcagatcg cgcgacaagc aacggtactc
    64501 gattcatcag atgatttcgc cgtagtcttt gctgctattg gaatcacttt tgaagaagct
    64561 gaatttttca tggaagattt tcgtcaaacg ggcgcaatcg accgttctgt catgtttatg
    64621 aatttagcaa acgaccctgc aatcgaacgt atcgctactc ctcgtatggc tttgactgct
    64681 gcagaatatc ttgcttatga aaagggaatg catgttttgg ttatcatgac agacatgacc
    64741 aactatgcag aggcattgcg agagatttct gcagcacggc gagaagttcc aggaagaaga
    64801 ggatatcctg ggtatcttta cacgaactta gctactttat tcgaacgagc aggaagaatc
    64861 cgcggactaa aaggatcggt tactcaaatt ccgattctaa caatgccaga agacgataaa
    64921 actcatccaa tccctgactt gactggttat attacagaag gacagatcat cttgactaga
    64981 gagctttaca agagtgggat tcagccacca attgatgtac tcccgtctct ttcgcggtta
    65041 aaagataagg gaactggacc cggaaagaca agagaagacc atgcagctac gatgaatcag
    65101 ctttttgcag cctatgcaca aggaaaacag gcaaaagaat tagccgtagt tttaggtgaa
    65161 tctgctttat ctgatgtaga caagatctat gcgaagtttg ctgagcggtt tgaaaaagaa
    65221 tatgtcaatc aaggattcta cacgaaccgt tcgattactg aaacacttga tctagggtgg
    65281 gaactgctat cgatgttgcc aagaaccgaa ttaaaacgaa tcaaagacga catgctggac
    65341 aaatatctgc cagaagggaa gtgagttctg atggcacgat tgaatgtgaa tccgacccgc
    65401 atggaattga ctcgattgaa aaaacagttg acaactgcca caagagggca taaattatta
    65461 aaagataagc aagatgaact gatgcgtcaa ttcatcctgt tgattcgaaa gaacaaccag
    65521 ctgcgtcaag aaatggaaga gtctatgaca gaagcaatgt ctgattttgt attggcaaat
    65581 gcttcgatca cggaaccttt catcgaagaa ttatttgttt tgccggcgga aaacgttgat
    65641 ttgtctatcg tagacaaaaa tatcatgagt gtaaaagtac ctgtgatgaa ttttgaatat
    65701 gatgaacagc tgacagacac acctttggaa tacgggtatt tgaattccag tgccgaactg
    65761 gatcggtcaa tcgatcggtt tactaccctg cttccaaaac ttttggaact gacagaaatc
    65821 gaaaaaactt gtcaactgat ggcgagtgaa atcgaaaaga cacgtcggcg agtcaatgca
    65881 ttagaatata tgacgatccc tcaattagaa gaaacgatct attatattcg tatgaagctg
    65941 gaagaaaacg agcgcgcaga agtaacaagg ttgatcaaag taaaaaatat gggtaccact
    66001 gactaagtgg tatctttttt taaaagagga aaacgatgat taaacaaagc aaacaaataa
    66061 agaaacgttt gccggctgtt cagcttctcg cattaggatt ttttctcctg attttagttg
    66121 gcggcggcct acttacccta ccatttttta gtaatagtgg gcatgggaca aattttatcg
    66181 atgcgctttt cactgcaact tctgctgtct gtgtaacggg attgacaaca ttgaatacag
    66241 cagagcattg gaacagtgct ggtcaatttt tgatcatgct tttgattgag atcggcggtc
    66301 ttggttttat gatggttccg attctatgct ttgcattgat aaagaagaaa gtcagtctca
    66361 gcacacgtat tatattaaaa gaagcgctga atctagaaga agtatcaggt gtgatgaagc
    66421 tcatgcttta catcctaaaa ttggcctttg ttatccaatt gattggtacg gttgctttat
    66481 cttttgtatt tgtgcctgaa tttggctggt tgaaaggtat ctggtacagt ttgttccatg
    66541 caatctctag tttttgtaat gcagggtttg atttgttagg agacagttta gccaatcacc
    66601 agcaaaatgt ttatttgatc atggtggtat ctgctttgat catttctggt gggttaggct
    66661 tcatcgtatg gcgtgattta ctggaatatc atcagaaaaa gaagatgacg attcattcaa
    66721 aaatcgcatt aaccgttaca ggtacccttt tattaggcgg attcgttgtg ttctttttca
    66781 cagagcgcaa tggggcaacg ctagtggaag ggaatatgtt tcaacgattg gcaaacacct
    66841 tttttatgag tgtgacacct agaacggctg gatattattc agttgattac ctccatatga
    66901 gccatgcagg tctgatttta acgatgtttt tgatgtacat cggcggaaca tctgggtcga
    66961 cggccggcgg attgaaaaca actacgttag gtgtgttgct gatccagatg cgttcgatgt
    67021 tgaaaggacg tacgagagcg gaagcatttg gccgtaccat ccgacaaggc gcagtgttgc
    67081 gggcacttac tttatttttt gttacgttga cgttgtgtgt agtcgccatc atgattttat
    67141 cggtgactga aacgatacct aaaacttctg gcatcgaata tattgctttt gaggtcttct
    67201 ctgcgtttgg tacagtaggt cttacgatgg gattgacgcc agacttgact gcaataggga
    67261 aattgatcat tatagcgctt atgtacattg gacgagttgg tgtattgact gtggtcttct
    67321 ctctgatgac aaaagcacaa agaacacaat caaaatacaa atatccagaa gaaagtgtca
    67381 tgattggata atttcatctt ctgacagaaa cctagtgatt tagtaaaata agaaaataaa
    67441 ataatttttc ttgcttcttg gatactttaa gtgcaaaatg ttcgtttatt ttttgaggca
    67501 ctacccgttg ttgaacctcg ggtagtgcct cttttttatt gaaaggaatc aataagattt
    67561 gattccttta tttatcaata ggttataatt catatttttt tattttttct tttacgatac
    67621 ttagtccaga agctaaaatt tcaaggttat tagaggatag ggaaattcga acgaatgcat
    67681 ctggttgcgc acgtgtagta aagcgtctgg aatgataaag tcggataccg ttagcaagaa
    67741 agtcattctc aattatctta gggtcaatct caggatcaac tggaagaatt cgatagtaag
    67801 ggacacgatt cgttggtttt tctactttga aaagagaatc aaaaagctga ttggctcttt
    67861 ttgtaagact taatttagtt tctaagatcc tatttgcttc atttgaatgg aggatttgag
    67921 tgatgacttc cacatctaat ccagaagttt tcacatttat attaaaaatt gcttgttcta
    67981 tcttgctttg gacatgtact ggaactacaa gataagcaac tcgcaatcca gaacaaataa
    68041 attttgtcat tccagctaag tagattgttt ggttaggtaa taaattttgg aaaggaggaa
    68101 tgatcgtatg ttgatagtaa gttgttaaaa atgaatggat atcatcttca ataacccata
    68161 gaccttcttt ttcaatcact tcggctaatt gctctcgtct tttgtgagag atttgaaacc
    68221 ctataggatt attacaagaa ggcataagaa agacaccatg aatcttcttt tttctacatt
    68281 cctgaatcaa tatatcaggt cgcattcctt gctcgtcaaa atcaattgga acgatctcta
    68341 aatgatataa ctgagccaat tcaatgaaat tagcgtatgt atatcggtct gttgctattc
    68401 tatcacctgg agagaaaagt gcagaaagtg tgatagccaa tccattttga acaccggaaa
    68461 caatagatag agattctaag gacggaatga ctccttgagt ttttaaccaa tttgctgcag
    68521 tagtcaattg aaaatttgtg cctttgggat cttggtaatt aagcaaacca acaagatttg
    68581 ggttattact agttgattga ataaacggga caacgaattt attacattct tcaaaagaac
    68641 taaccagacc aaaatcaatg atatgttcgg ttacttcatt ggtagagatg gtgatggact
    68701 caaagacatt gggagaaata aatgtgccac ttccaatatt cgtatacagc aaacctttct
    68761 caattccata tttataagcc ttgccaacgg tagtaaaatt taaatctaaa taatcagcta
    68821 gctctctttg ggagggtaac tgagtgtttt tctgtaattt attctttaaa atatcttgtt
    68881 ctaattgttg aatcaacgat cgataataag gacgcttcaa tttttgtttg tctggtcgcc
    68941 aagttaacgg gtaattgtca taagaattaa taggcatttt ctccactcct taaaattgta
    69001 taccatacaa ttttaccatt gattgtattg attttagaag ttaatctttt gtgaggaggg
    69061 aattgtctat gaaaattatt aattttgagg aagtagtagc ttcttttagt tttgaagaag
    69121 cgattcaagt aatgaaaaaa tgtttttttg attatgaaag tggtaaaatc agccaaaatc
    69181 ctcgattggt ggaaatttta cctgacggaa aaggcaaaaa tatgtttgcg atgatgcctg
    69241 cttatttggg ggagaacaga tattttggca caaaagtcat taccgctttt ccggataatc
    69301 atcagtatca tttaccttct catttaggag agattttctt gtttgatgct caaaacggac
    69361 tacccaaagc attgataaac gcaaatgcag tcacctggat tcgaacagct gctgtatctg
    69421 cgctggcaac agattactta gcaaatccgc atgcagaaac acttgcttta attggagctg
    69481 gtcaacaagc attgtcgcat ttgactgcga tttctaccat tcgtcccatc actacagtgt
    69541 ttgtttacga tattttagaa gaaagacgaa atacttttat cgctaaagct aaaaaacagt
    69601 atcctaaaat aacgtttata aatgcaatat cagtagaaga tgcagtacgt ccagcggaaa
    69661 tcatttgtac gttgacctct agtaaagagg catttcttca aaaagagtgg gtaagggaag
    69721 acgcacatat caatgctgtg ggcacattta ctcctgatac aagagagata agtacatcgt
    69781 tgatggaaac ttctcaaatc tatgtagatg attactcagc tgccttaaca gaaagcgggg
    69841 acctgcttat agccatggca gaaggagatt tagaggaatc ttgtattgtt ggtacattag
    69901 gcgaattagt ttctgagaaa aaaacatttt ataaaggaga atcgaggccg acgattttcg
    69961 acgcagttgg tctagctatt gaagatcttt gttgtgcaga atatatttat aacaaattag
    70021 agaaaggggc tttaaaatga acttagatta ttacgtagtt gatgcatttg cagatgaggt
    70081 gttcaaagga aatccagcag cggtttatgt gctagaagaa tggctgccag aaggtacgat
    70141 gcaaaaaatt gccattgaaa acaatttatc tgaaacagca tttacagtga agaaaaacca
    70201 agaatttgag cttagatggt ttacgccaga tcgagaaatc gatctttgtg gtcacgcgac
    70261 attagcaaca gcatttgttt tatttaacta ttacaaaata cctgatgaaa cgattaaatt
    70321 ttctactcaa agcggtaatc tatttgtgac aagaattaaa gattattatt acatggattt
    70381 tcctagtatt atgccgaaaa aagtgccgat attgtctgaa tatgaagaag cgattggggc
    70441 taagattaaa gaagcctact tagcacgtga tttatttttc gtattagaag atgaaaaaac
    70501 ggtggctaaa ttgcagccag attttacagc tatcaaaaat tttgaattag gaataggagt
    70561 catcgtgaca gcagaaagtg tgcaaaaaga ctttgtttct agaaccttct ttccaaaatt
    70621 aacaatcaat gaagatccag tatgtggatc agcacattct aatcttattc cttattgggc
    70681 agaaagaatg aataaaacaa aattgtcagc ttatcaaatg tcatcaaggg gcggggatct
    70741 agaatgtgaa ttaaaagaag atcgtgtgat aataggagga aaagcagttt tgttttctaa
    70801 aggtacctcg tttatttgat ttctttcaaa tgaattaaaa ataaattggt ggagagtggc
    70861 aaccttatag ctacaatggt tgttaaaaaa agacgcaaac tttttcaatt taatgaaaag
    70921 tttgcgtctc attttaatta ttggtgtttg ttttttattc aatcaagagt aaaatgcccc
    70981 atactaacta tcctcggatg acttcgattt tataaccatc agggtcaatg acaaagtagt
    71041 aagaaggcgc agtgccaggt aatcctttta agtcagtaat ctcaagaccg gcagctttat
    71101 gttgctcgtg taatttttct agatcatcag ctgctatagc gatatgacca tagccgtctc
    71161 ctagatcata cgcaccatga tcatagttgt aagtcaactc taattcatag tcatccccag
    71221 gaagtgtcaa ataaacaagt gtaaatttgt tttctggaaa atcacggcgg cggctttctt
    71281 caaaaccaaa tgccttttga taaaattcga ttgatgcttc aagatctttc actcgaacac
    71341 atgtgtgagc cattttcata gattcatctc tccttttcct tttactcaat cataacaaaa
    71401 agaagtaagg aaatagttga tttgtgcttg ttcatgagaa atttctcatg taaaatggat
    71461 agaaaataaa actgttaagg tggaaactat tcatggataa accaattttt ggtgaaaaaa
    71521 agcaatttgc tgattatcaa acccgttatg cagcatatat tgtcatagaa agagatcagc
    71581 agattgcagt aatcgaggct ccaaatggtg ctttttttct tcctggcggg gaaattgaag
    71641 gagaagagac gaaagaagaa gcgattgtcc gtgagctgat agaagaaatg ggaattgtgg
    71701 cagaaatttc ttcttatctg ggagaagctg atgagtattt ttactcgaat tatcgtgcaa
    71761 cttattatta taatccagga tatttctttg ttgctgattc ttggaagcgt ataggagaac
    71821 caactgaaaa gaccaataaa atttggtggg tgagccctca agaagcgctt gtgaaattga
    71881 aacgtggtag ccaccgatgg gcagtcgaga gatggatcga aaaaaataaa aactgaaaga
    71941 aagcgagtag atcagctttc tctcagtttt tttatttatt acagtacata ttttttgatt
    72001 gcttcagcta caccgtgctc gtcgtttgat gaagtgatga catttgctgc ttctttgaca
    72061 agagaaacgg catttcccat tgctactcct agaccagcat attcaatcat ggataagtcg
    72121 ttctcgttat ctccgatagc catgatttca tcttgatcaa taccaaggtg ctgtgcgaga
    72181 ttcgcaactg ccgcaccttt acttgcttct ttatttagta cttcgaagta atagggcgca
    72241 cttttgaccg ttgtgtattt atcacggaaa gtttgcggta aacgcgcaat tgctgcatct
    72301 aaaatttctg gttcgtcgat catcatcatt ttgacgatgt tcatgtctgg tgtcatttct
    72361 tcaggtgttc gatattttag cggcatcttg actaaacttg cttcgtggat cgtataagcg
    72421 ctgatatctc gattaggcgt gtagatcgct ttgtcatcga tcgaatggag atgactgccg
    72481 acacggcgtg ccatcacttc gatctctagg aaatcttcat gtgtcagtcc gtgacgggcg
    72541 atgattttac ctgatttcgt ttgttgtacg agtgccccgt tataagtaat aacgtaatca
    72601 ttatcttgaa aaaggttcaa ctcgtttaac tgttcctgga cacctggtaa gggacggcca
    72661 gtgcataaaa cgatcgaaac accttttgca acagcagcgg caatcgcttc ttttacttct
    72721 tttgttactt ctcttttctc atttaataaa gtaccatcga tgtcgatagc gactagtttg
    72781 atagacataa tttcctccaa tatattttaa tttatttcaa tcaatgcgcc attacggata
    72841 tggctggcaa attcttgata agtttcgtca aataaatcat aatgatcacg caaggacgga
    72901 tcaaccattt cttttggaaa gaaaaatcgc tcatctcctt gttcttgtcc cgctaatgca
    72961 gcaacaagct gactaacctg tgataattct atcaaagttc cgtcatttcg caaaatctcg
    73021 atttgtgtgc gatgtctgcc ttgttctggg cgataaaaat cataaggtag atcgtagcta
    73081 gaattcacag cagtgtaata aacaggatta tatccgacct tttctactaa caaagtgagt
    73141 tcttggacta attttgaatc ccggttgcca gaaaaagtag cagatttcag tggtttacga
    73201 ttcaaaaaac gtttagctaa atcatttaaa ataggatcag cagattcaga ccattgagta
    73261 aagtaagtgc ccaagacccc atcatcaagt ttgaggtatt cttctaaagt aaagcttccg
    73321 tcaagaaatg gcagaagtaa ttgagaatgg agttcaaaac tgctggcatc ttcttgatac
    73381 aactcgtgtg cgcgatgaag cagatgctcc aaaattactt ccataccacg ggaaacagga
    73441 tggaagtaca cttgcacata catttgatat cggctaacga tataatcttc cactgcatgc
    73501 atgccactca tggaaaaagc gatcccgcct tcataaggac gaatcacacg taatattcgg
    73561 gtaagatcaa atgttccata ttctgttcca gtgaaataag catcccgaag caaatagtcc
    73621 attcgatctg catcgatctg actagaaatc atttggacaa cttgtgggtt ggggtaagtt
    73681 ttttggatca cactagctac cttttcagga aaaccttctt ctactcggtt aagaatctga
    73741 tacacttctg tttctggaga agtgatgatc tctacagtga tggcttcatg attcgtatgg
    73801 aagatatgtt caaatgtatg agagtaagga ccatgaccga catcatgcaa cagtgccgca
    73861 cataaagtca ccagccgttc ctgatcatcc cagcctccgt tgcctatttt ttcttttgaa
    73921 aaatttcgag aaaagatatc gcagattcgt cttgaaatct cataaacgcc taatgaatga
    73981 gtaaaacggc tatgttctgc tccgtgaaaa gtgaaagaag aagtgcctaa ttgtttaatg
    74041 cgtcgtagac gttggacttc tttcgaattg atcaaatcta agattacttg atgctggaca
    74101 tggatatagt tatggactgg atcacgaaaa actttttcca taggcagcat ttgatattta
    74161 taaggggttg tcataatgta gctcctttat gttattacgt tgttccattc tctggatttg
    74221 gttgttccat tgctccagct ggatttcttt ttttagccag tcggtagaat cctgtaaatc
    74281 acttgcagaa tacagctttt caaaagcttt tgcaaatgct tctttgactt cttttacaaa
    74341 aaggggttgg tcaagcagtt cttcaagtgt tgccattgta gcaggatcca ctgcaggata
    74401 gccatttgta ccaaagtctt cttttaaagc agattgataa aatcgcttca tgacttgccc
    74461 gcgtttttcc tgattaccgt tgacacttaa gtacatcata acagaaatcc catttttgat
    74521 ccgacgctga gcgataccgg caaatttttt gccatggatg cttaaatcat aagttccagg
    74581 acaataagaa tgggtgattt cgaatgcttg tatcggtgcc tttggaaaag cgtatttagt
    74641 caataggagc atttgctcgt aagcgtcatc tatggttatt ttttttgtgt cactttgggg
    74701 gaaaatcaaa gaaatattca aaattccttc atctgatacg atccctaatc cacctgaatt
    74761 tctgatcact ggagcgtaac ctgcatcttt cagtgtttgg atcccgtcac ttagaaaagg
    74821 agttcgaaca tccttcatac ctaagatgac tgtgcagtca tattgccaga aatgtaaaaa
    74881 gatttgctgg tgttctttag aatattccgt taatatatct gttaatgcaa aaggggaaaa
    74941 gtcgtttttt tgataaatgt gctgatctag tcgaatcgca gggttcattc aaccggctcc
    75001 tttgtataaa ttcatatttt ctacaatatt atactctaaa atcaaagagt tgttttggaa
    75061 aaaaggtttt ctgtaaaaat ggacaagttg gttgacaaaa caaaagaaac taggaacaat
    75121 gtagctatga gaagtaaagg agaatgaata atggaattat cacaaggaac accgatttca
    75181 atcaaattgc gtacaaaagt aaaacagcaa ggggaagtgc aagattttta ttttgatttg
    75241 aaagggcaaa tggtgacaat tggcgatacg ctgtacatac gttataaaga gattcaggag
    75301 gagacgggcg atgagattcc tgtaacgatc aaattagttc cagatggtca tgtacagctg
    75361 atccgtgcag gagaaatgcg gatgcgtttg aaatttggct ataaagaacg gttagaaaca
    75421 tcctatcgaa caccatacgg tatgatccag attgctactt ttacgaaaga actgcatgtt
    75481 agtttgaaag accgtcccac aggagggaag gttcgaatcg attatgatct gttcatggga
    75541 ccggaaaaaa tcggcgaata ttatttgacc ttggatttta cagcgtaaat ataaaagaaa
    75601 agtttgattt tttgtgtctg attttgtatg ataaggagta gactgtgaaa ggacgtgtcc
    75661 tgattggaaa ttagtgtatt tgaaggtgca aacaaaagcg aattatcaat gatcgaagta
    75721 gcacacgcga tcttagaaca acgtggcgat gtcatggatt tttctgattt agctaaccag
    75781 atccaaaact atcttgggaa atcagacagt gaaattcgtg attcactcgc acaattttat
    75841 actgacttga atattgacgg cagctttatc tcattagggg ataatcgttg gggcttgcgt
    75901 tcatggtatg ctatcgattc gatcgatgaa gaagtaaacc atggaatgga cgaagaggat
    75961 gaagatactc cacgtcgccg taaacgcaag aaagtcaacg cctttatcaa tgatgatgaa
    76021 gacgcgatcg attacaatga cgatgaccca gaagatacag atctgacaga agaagatgac
    76081 gatgatctgt ttgatgacga tgatgatgaa gatgaagaaa tcgctgctta taattctgac
    76141 ttgcaagaaa tcggagcaga tgacccgaca gatgatgaag aagacttgcc aggaaatatc
    76201 gaagaagatt taagtatcat agacgaagac gatgatgatg aagattatga agaggaagaa
    76261 ttttctgact ctgaaaaaga ataaacatta ttttcttacg agaggttgtg acagagacga
    76321 tcagcctatc agaataagga ggtgtgacac gatttttgtc acaccttctt ttttgtatat
    76381 aaaaatccca agtaaataca taaaaaataa aaaaattgaa acttttagcg caccttgtac
    76441 gtcatattaa gtgaggcaga aaggagtgga gaatgatttt tactggatat aagaaaaaac
    76501 ggcagatcga aaaataccgt cacctgctga atggactcat cgaaactgat tatcaaaaaa
    76561 tgttccgtat cacgatgaat tacgtacatg ataaagaaga ggccttagat gtgatgcaag
    76621 atagttttca taaagccctg aactattttg aaaaagaaaa agagatcgag cattttagtg
    76681 catggtttta tcggatattg atccgcacgg cattagatag ttggcgcaga caaaaaagga
    76741 cgcctgtgga cttatttgaa attcaagata tcccccaaca gttaccttta gaaacttccg
    76801 ttgcagagct ccatatgatt ttagataaaa tagacagtcc ggaaaaagag attctgatcc
    76861 tctacttttt tgaaggattt cgtttgaaag aaattgcgac tatcttggat atgaatgaaa
    76921 acacagttaa aacgaaaatg taccgctcat tgaatgcttt acgtcaaatc ctagaatgaa
    76981 ggagagaaga gtgccatgta ttcccaaaaa gaaaatttaa aaaaactaaa gaaaacttat
    77041 aaagaacaag caattcccaa agggcttaaa gaggagatcc gccagcgttt tattatcgaa
    77101 gagcaatcat ttataaaagc aaagcggaga aagagacgtc tccaagggtt cggagtatct
    77161 ttggctatcg taatgattac aacaagtagc cttcttttta atagtcaagt tcgaagcttt
    77221 gcagaagatt tacccatctt aggatcagtc attgaattga tcttaggcga acgctttact
    77281 gatcgatctg aaaaaatcga tattcaggta ccgaagatca atacacaaaa tgagaaagaa
    77341 aataaaacaa ttcatggatt aaatcaaaaa tactttcgcg aaggacaagc agattttgaa
    77401 aaagctgaaa aagaatatgg aaattttgaa acggatcatt accaagtttt gggagattat
    77461 cagaaaatcg tagacgataa ccggtttctc gtgattgaaa ggcagatcac ccaaacagca
    77521 gcagatagcc atgttgaaaa aagatacgac acaatcgaca agaaaaacag tgtccagctc
    77581 tcacttccac ttttattcaa agatgacacc tatctatctg ttttgacaaa agaagtcaaa
    77641 cggcagatgg ccgaacaagt caaggaagat ccatctaagt actattggac tgagcaagat
    77701 attcaagaag ggacgataga aaaaccaaca cttgttacgc ctacacgaag tttttatttg
    77761 aacaaagagc atcaattagt tttgactttt tctcaatacg aaattgctcc tggttatatg
    77821 ggtacaccag aatttgtcat tcctaagtcc gtgacaaaaa cgattttggc atcggaagat
    77881 tatttggatc attagacgag aagtagttgc gactagcagt ttcaattttc aagtaagtac
    77941 aagtaaataa taagaagaga aaaagagaaa gaagagatat agtgaaaaga aattatttag
    78001 ttatcagtat tattgcttta tccgtgcttt tgtttttcga aattacttcg gtggcctcct
    78061 tgatcaccga agtaaaagga caaacacact cttcggagca ttcgtcacaa tctttcaaac
    78121 aatcagaaca atcaaccatt aaaagccaat caactgaaac agctaacgtc aaagaagcca
    78181 ttcaagaatc agcggaaaag gtacaaagtc agtcatcaca atcaacacaa gacagtacaa
    78241 aaacgagccg agatccggct gtcaaaagcg tcgcaatcag ctttgatgat ggaccaggag
    78301 cgaccacaac accgcagctt ctgcgaatct taaaagaaaa aaacgtgcat gccacttttt
    78361 tcgtcttagg agaaaatacg gcgcagcatc ctgaaatcgt gaagcagaca gctgaagcag
    78421 ggcacgagat tggcaattac acatacgatc atcagaatct ggccactctt tctgcccagt
    78481 cgatgacgga ggaagtgacg aaagcagaca ctgagatcaa aaaagtcact ggcaaaaccc
    78541 ccacttttgt tcgtccgccc tatggttctg tcacgaatgt aggagcaacg atcatccagc
    78601 gaccgatcat cgaatggtct gttgattcag aagattggaa gacaagaaat cctgatttga
    78661 ttttgcagaa gatccaagca acagtatacg atggggcaat tattttattc catgatatct
    78721 atcctgaaac gattcgtgcc gttcctcaag tgatcgatta tctgaaagag caagggtatc
    78781 gcatcacgac agttggggat ttattagggc atccaacggc tgtcgagaac tattatggca
    78841 gaaacgatca tcggccagtt cagtaagtga gacaagtatc tttaataaat aacacatttt
    78901 aataagtagt tgaacacata actaccttac ttgcaaaggc ttaacaatat ctatacaggt
    78961 acaacaaaaa actgtccacc aattttcttt ggcggacagt tttcacttaa aaataaagaa
    79021 gcaactattc gtaaattatg ctaaaatagc agtagaacgc gtcctgaggg aatcgaaccc
    79081 ccgtctcaag aaccggaatc ttacgtgata tccactacac taaggacgca agcactggga
    79141 taattgtaac taaacagata aaaaattaca aggggattgg acaaaaaaat gaaatttgag
    79201 caaaattttt cacaaaaaca gcagcagaca cagaaactag ccatgacgca acagttgcaa
    79261 cagtccatcc aagttttaca atataacagt gaggagctgt tggcattcgt tgagaatcaa
    79321 gcaatggaaa atccattggt ggaagttgtt gagccggaat ggcagccaga ttacataaaa
    79381 gcttcttcat cttcttatga aggagaagag acaaattacc taaatcagat tcctgataca
    79441 aaagggtctt tatttgattc attgatcgaa caagtccatt tgaattaccg agatactttt
    79501 ttgaggaaaa tcgttcttta tcttgtagaa tacatcgatt tgaacggatt tttgacgatt
    79561 agtttagaag aagccggaaa agagattgga gctacgccta tccaaatgct cgatgcgttg
    79621 actctgattc agcaattaga accagctgga gtaggtgcac gtagtttgca agaatgtctc
    79681 atgcttcaaa cagaacgaga tgattatgcg ccggaattag cttatatcgt gttggaagaa
    79741 tgttttgagg aactggtgga gcgaaaatgg aaagaaatcg cacaacgttt ttctgttgat
    79801 ctacatgctg ttcagcagat attcgactat ttacaaacgt tgagtccaag ccctggccgg
    79861 attttcgacc gtagcagcga attgtacatt attcctgatg tccgggtatt agtggacgac
    79921 gataaaaatg tacaagttat ctcaaaccga aaaaatcagc caaatatacg ctttcaagag
    79981 agttacttca aacaaatgtc acaacaagca gataaagaaa ctgaaaatta tttaaaagaa
    80041 cggaaacagg aattcgaatg gttgaagaaa acgatcctac aacgtgggga tacaattttg
    80101 cgggttgctc aagtcattgt ttcccggcaa aaagctttct ttatcgacaa ggaacgtcca
    80161 atcaagcctc ttacgttaaa ggaagttgca actgagatag atgtgcatga atccacggtc
    80221 agtcgtgcag tcaacgggaa atatttggaa acagatttcg gtgtatttga attaaaaaaa
    80281 tttttcacta cacgaattcc tacgaatagt acagaacaga cagaagacct ttcagcagat
    80341 acagcgaaga aaaagctgca ggaattagtc gatcaagaag acaaaaacaa gccgttgtcg
    80401 gaccagaaat tggttgaatt gctgaaaaaa gatgagatag cgatctcaag acgaacagta
    80461 gcaaaatatc gtgatttatt aggaatcccg agttcatcaa aaagaaaacg ttatgataac
    80521 taatcagtca aaagactaga aaaagtattt cactttttct agtcttttta gttgaaaaac
    80581 gagagcgaaa ctgttagact tacagtcgtg aggttgagat ttccctttat tgatgtgaaa
    80641 ttcgaatagt taatttcttt ttttatcgta tatgggtcgt tttaagtcta cattggacgt
    80701 aaagtgtcca actaaggaga tcgctatgct ggatgaaata aaaatgattg aatctgttgc
    80761 accagatatt atcgaagtgt tgcaagaacg ctataaaatt ttacgaaata tctattggat
    80821 gcagccgatt gggcgtcgaa gtttatcgga aagtatgggg atcacagaac gagtcttacg
    80881 aacagaaaca gaccttttga aaaatctgaa tttgatcgac acctcaaaaa gcggtatgac
    80941 gctgacaact aagggcgaag aagtttatca aagccttgaa aacttcatgg atcagctttt
    81001 aggtacgcat caaaccgaac aacagttggc agaatatttt ggtaaccaac gctgtatcgt
    81061 tgtttcagga aacagcgaag agcagaccaa agtggctgat gcattcggcg aagcgctgag
    81121 cgaagcctta gaccgactac ttccagaagg cgagaatatc atcgctgtga tgggaggaac
    81181 tactatggct gtggtagccg aacaattgtc taatctggaa aataagaaac ggcataacct
    81241 gtttgtccct gcaagaggcg ggattggtga agcgataact gtacaggcaa attctgtcag
    81301 tgcaagaatg gctgctaaag caaacggtaa ccaccgcgca ctttatgttc cggaacaatt
    81361 aagtctggca acttacaatt ctttactcaa tgagccatct atccaagaag tcttaaactt
    81421 gattagtgaa gcgaattgtg ttattcatag tattgggcgt gcattacaca tggcagcacg
    81481 tcgtaagatg actgaaaaag aattagtcat gctgaaacaa gcaaatgctg ttgcggaatc
    81541 ttttggctac tttttcaatg aaaaaggtga agtcgtttat aaggtcccta gaatcggcat
    81601 agaattgcaa gatttggaaa aagtcccgat catcatggca atcgcgggcg gaaaatcaaa
    81661 agctaaagca attcgagctt atatgaaaaa tgcaccaaaa caaacgtggc tcatcactga
    81721 tgaagctgct gcaaatgaga ttttaaaagg ggtaaccctt taaaataaat atttttttga
    81781 ttttcataag gaggaaatct taaatgacag ttaaagtagg tattaatggt tttggacgta
    81841 ttggacgtct agcattccgt cgtatccaag atgtagatgg aatcgaagta gtagcaatca
    81901 acgacttgac agatgctaaa atgttggcac acttgttaaa atacgacaca actcaaggac
    81961 gtttcaacgg aacagttgaa gttcatgaag gttcattcaa cgtaaacgga aaagaagtta
    82021 aagtattagc taaccgcaac ccagaagaat taccatgggg cgaactaggc gtagatatcg
    82081 ttctagaatg tactggtttc ttcacttcta aatcagcagc tgaaaaacat ttaacagctg
    82141 gtgctaaacg cgttgttatc tctgctcctg gcggaaacga tgttccaaca atcgtttata
    82201 acacaaacca cgaaacatta actggtaaag aaacagttat ctcaggtgct tcatgtacta
    82261 ctaactgttt agctcctatg gctaaaacat tgaacgacaa atttggtgtt gttgaaggat
    82321 taatgacaac tatccacgct tacacaggtg accaaatgac tctagacgga cctcatccta
    82381 aaggtgactt ccgccgtgca cgcgctgctg ctgcaaacat cgttcctaac tcaacaggtg
    82441 ctgctaaagc tatcggttta gtaattcccg aattgaacgg taaattagac ggagctgctc
    82501 aacgtgttcc tgtaccaaca ggttcattaa cagaattagt aacagttctt gaaaaagaag
    82561 taactgttga cgaaatcaat gcagcaatga aagaagcttc aaacgaatct tacggataca
    82621 acacagacga aatcgtttct tcagatatcg ttggtatgac ttacggttca ttatttgatg
    82681 ctacacaaac taaagtaatg acagttggcg acaaacaatt agttaaaact gttgcttggt
    82741 acgacaacga aatgtcatac actgcacaat tagtacgtac tttagaatac ttcgctaact
    82801 tataagatag acaaacgtac aagtgtaaat tatagacatg tacatttaaa agcggggaag
    82861 caatcgcgct tcttccgctt tttttgataa taagtttatt caggaggaaa taaaatggct
    82921 aagaaaacag tgaaagatat cgatttaaaa gacaaaaaag ttcttgtccg tgttgacttc
    82981 aacgttcctt tgaaagacgg cgtgatcact gacgacactc gtatcaaagc agcattgcca
    83041 acaatcaact acgttctaga acaaggcgga aaagcaatcc ttttctctca ccttggacgt
    83101 gtaaaaacag aagaagataa agaaggaaaa tctttagctc cagttgcaaa acgtctaggc
    83161 gaattattag gtaaagaagt aacattcgta cctgaaactc gcggagaaca attagaagaa
    83221 gcaatccgca acatgaatga cggcgacgtt gtcgtattcg aaaatacacg atttgaagat
    83281 attgacggta aaaaagaaag caaaaatgat acagaactag gtaaatactg ggcttcatta
    83341 ggcgatgtat ttgttaacga tgctttcggt acagctcacc gtgcacatgc ttctaacgta
    83401 ggtatcgctt caactggtat cccaacagtt gctggattct tgatggaaaa agaaatcaag
    83461 ttcatcggtg aagcagtaga agaaccaaaa cgaccaatga ttgcaatcct tggtggcgca
    83521 aaagtttctg ataaaatcgg cgtaatcgaa aacttgattc ctaaagcaga taaaatcttg
    83581 atcggtggcg gaatgactta cacattctac gaagcaaaag gaatcaagat cggtaactct
    83641 ctagtagaag ctgataaagt ggaactagca aaagaattaa tcgaaaaagc aggcgacaaa
    83701 cttgtcttgc caatcgacaa tgtctgtgca ccagaatttt caaatgatgt tgaaacacaa
    83761 gtaatcgaag gagacattcc tgatggtttg atggctttag acatcggccc agcatcagta
    83821 aaattatttg ctgatacatt acaaggtgca aaaacagttg tttggaacgg acctatgggt
    83881 gtatttgaaa tgagtaactt tgctaaaggt acaatcggtg tttgtgaagc aatcgctaac
    83941 ttagaaggcg caacaacaat catcggtggc ggagactcag ccgcagcagc agaacaatta
    84001 ggatttgctg acaaattcac acacatctca acaggtggcg gcgcaagctt ggaattgctt
    84061 gaaggtaaag aattaccagg actagcagca atcaacgata aataaacgac caagtttgct
    84121 caattctgac gagtaatcga acaaaaggtg aagggagttt tcatgactcc ctagccattt
    84181 ttcaataaat taaggagtgt gttcctcaat gcgtaaacct attatcgcag gtaactggaa
    84241 aatgaacaaa actgcatcag aagcaaaaga atttgctgaa gcagtaaaaa acaaaatccc
    84301 ttcaaatgac aaagttgatt cagtcatcgg atctcctgca ttattcttac aagaattaat
    84361 gacatcagct gaaggtacag aactaaaaat ctcagcacaa aactgctact gggaaaactc
    84421 aggtgcgttc actggtgaaa cttcaccagc agctcttgct gatctaggtg ttcattatgt
    84481 gatcatcgga cattcagaac gtcgtgaata tttccatgaa acagacgaag acatcaataa
    84541 aaaagcgaaa gctatctttg caaatggtat gacaccaatc ctatgctgcg gcgaatcttt
    84601 agaaacctat gaagcaggca aaactgctga atggatcgaa ggacaaatta cagctggttt
    84661 agctgatctt tcagctgaac aggtgtcaaa catggttatt gcttatgaac caatctgggc
    84721 aatcggtact ggtaaatcag ctgacgcaca aatcgctgac gaaatttgtg gagttgtacg
    84781 tcaaacagtt gaaaaacttt atggaaaaga agtatcagaa gcagttcgta tccaatacgg
    84841 cggttctgta aaaccagaaa atattgctga atacatggca aaagaaaatg ttgacggtgc
    84901 tttagtcggt ggcgcaagct tgcaagcaga ttcattcttg tcattgttag acgctgtaaa
    84961 ataattgttg aaaatttgcg aaaaatgcaa gaaatgtttg cactaatgag caaaattaac
    85021 taaaatcgta tatgagggaa atcccttagg aaaaaacaaa ctcaaaggag agacaaaaca
    85081 tgtcaattat tactgatgtt tacgctcgcg agatcttaga ctcacgtggt aacccaacaa
    85141 tcgaagtaga agtatacaca gaaagcggag cttttggccg cggaatggtt ccttcaggtg
    85201 cttcaactgg tgaatacgaa gcagttgaat tacgtgacgg agacaaagct cgttatggcg
    85261 gtaaaggtgt aactaaagct gtcgacaacg taaacaacat cattgctgaa gcaatcattg
    85321 gctacgatgt tcgtgaccaa atggctatcg acaaagctat gatcgcttta gacggaactc
    85381 ctaacaaagg taaattaggt gcgaacgcta ttttaggtgt ttctatcgct gttgcacgtg
    85441 cagctgctga ctacctagaa gtacctttat accactacct aggcggattc aacacaaaag
    85501 tattgccaac accaatgatg aacatcatca acggcggatc acatgctgat aactcaatcg
    85561 acttccaaga attcatgatc atgcctgttg gcgctccaac attcaaagaa gctctacgta
    85621 tgggtgctga agtattccac gcattagctt caatcttgaa agctcgcgga ttagcaactt
    85681 ctgttggtga cgaaggtgga tttgctccaa accttggttc aaacgaagaa ggttttgaag
    85741 taatcatcga agcaatcgaa aaagctggct atgtacctgg taaagacgtt gttcttgcta
    85801 tggatgctgc ttcttcagaa ttctacgaca aagaaaaagg tgtttacgta ctagctgact
    85861 caggcgaagg cgaaaaaaca actgacgaaa tgatcaaatt ctacgaagaa ttagtttcta
    85921 aatatccaat catctcaatc gaagatggat tagacgaaaa cgactgggac ggattcaaga
    85981 aattaactga cgtattaggc gacaaagttc aattagttgg tgacgacttg ttcgtaacaa
    86041 acactcaaaa attatctgaa ggtatcgaaa aaggaattgc taactcaatc cttatcaaag
    86101 tgaaccaaat cggtacatta acagaaactt tcgaagctat cgaaatggct aaagaagctg
    86161 gctacacagc agttgtatct caccgttctg gtgaaacaga agattcaaca atctctgata
    86221 tcgctgttgc aacaaacgct ggccaaatca aaactggttc tctatcacgt actgaccgta
    86281 ttgctaaata caaccaatta ctacgtatcg aagaccaact tggtgaagtt gcagaataca
    86341 aaggtttgaa atctttctac aacttaaaaa acaaataatt cttttctaat gaagatacat
    86401 gatcgactat tgaaagatag tcgagtatga tcacttagtt aagtaatcga actctctact
    86461 tgctgaaata tcagcaggta gagagttttt ttgcaactag cttctgtata tgatgtttac
    86521 aaagaatggt acaagaaaag atcatccttg aaaaaggaaa gctaacaata actagcatca
    86581 tataagagct tttaaggata gaacagtaga ataggaatct atgggcaagt cctctcgtat
    86641 aaataaaata ggagggaagt agcagcatat ttttcataaa gtggctctct ttcttcattt
    86701 ttactttggt tgctcctatt tgaaaattcg agcacactca aatacaataa aaagaaatgg
    86761 caagggagag gattagatga aaaaagaaga aaaaatcgcg attttacaag agattatccg
    86821 aattaaatca gtgaatggaa acgaaggtga agtagcagcc tatttgaata agcttttggc
    86881 aaagcatgac ataacaggag agattgtttc ttacagagat ggtagagata atttgattgc
    86941 tcgttaccaa aagggccaat caggaaaagt gttaggccta tctggacata tggatgtcgt
    87001 ggcggctggt gatgaatcgt cttggaccta tgcacccttt gcagcagaaa tccatgggaa
    87061 ccgtttgtac ggacgtggtg ctacagatat gaagagtggc ttggcagcaa tggtgattgc
    87121 catgatcgaa ctgaaagagt caggaaaacc atttaacgga acagtcaaat tattagccac
    87181 tgtcggagaa gaagtgggtg aactaggtgg ggaacagctc acgaaagcag gctatgtaga
    87241 tgacttagat gccttgatca tcggagagcc aaccaattac agtttgatgt atacgcacat
    87301 gggatcaatc aactatacag tcacctcgca cggaaaagaa gcgcatagct ctatgccaga
    87361 ccaaggttat aatgcaatca atcacctaaa tgaattcatt acgaaagcaa atgctgaaat
    87421 gaatcacctc gctgaaacaa tcgagaatcc tgtcctagga aaaacaatcc ataatgttac
    87481 attgatcagt ggcgggaatc aagtcaacag catcccaagc catgcgcagc tccaagggaa
    87541 cattcgatcg attccagaat acccaaatga taaaatcatt gcattgctgc aatcaatcgt
    87601 caatgaattg aatcaagaga cagactatca tttggaattg atgatcgatt acaacaaaat
    87661 acctgtcaaa gctgatccag attctccttt gattcattgc attcagcaac aattcagcca
    87721 gccattacct ttagttggag cggcagcaac aacggatgca gccgaattta cgaaagctaa
    87781 ccattccttc gactttgtcg tctttgggcc tggtgtcgtt actttgcctc atcaagtaga
    87841 tgagtatgtt gaaatcgata attatttgga tatgatcgaa aaatatcaag gaattatttt
    87901 atcctactta gcgtaagatt tttttaat
//
LOCUS       DAJSBL010000004        80436 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.004, whole genome shotgun sequence.
ACCESSION   DAJSBL010000004 DAJSBL010000000
VERSION     DAJSBL010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 80436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 80436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..80436
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.004"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..235
                     /locus_tag="ONT85_000350"
     CDS             <1..235
                     /locus_tag="ONT85_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HCR2377350.1"
                     /translation="SGKSLEALELEQEESIRFQNCSLFPLYHGSAKSNIGIDNLIEVI
                     TNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAY"
     gene            470..706
                     /locus_tag="ONT85_000351"
     CDS             470..706
                     /locus_tag="ONT85_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2377351.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            713..2665
                     /locus_tag="ONT85_000352"
     CDS             713..2665
                     /locus_tag="ONT85_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HCR2377352.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(2737..3234)
                     /gene="dfrG"
                     /locus_tag="ONT85_000353"
     CDS             complement(2737..3234)
                     /gene="dfrG"
                     /locus_tag="ONT85_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HCR2377353.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            3596..4636
                     /locus_tag="ONT85_000354"
     CDS             3596..4636
                     /locus_tag="ONT85_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HCR2377354.1"
                     /translation="MYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLPQRK
                     KIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLGKVQ
                     MEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVSPLP
                     LGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYSPVS
                     TPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIVNTQ
                     LKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRLNSR
                     IDKVRYMFNKIT"
     gene            4734..4922
                     /locus_tag="ONT85_000355"
     CDS             4734..4922
                     /locus_tag="ONT85_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HCR2377355.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(4980..5333)
                     /locus_tag="ONT85_000356"
     CDS             complement(4980..5333)
                     /locus_tag="ONT85_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR2377356.1"
                     /translation="MRKKEDKYDFRAVGLAIKEARMKRGLTREQVGTMIEIDPRYLTN
                     IENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLM
                     EALAKGINEARNIEG"
     gene            5864..6286
                     /locus_tag="ONT85_000357"
     CDS             5864..6286
                     /locus_tag="ONT85_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006733729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HCR2377357.1"
                     /translation="MKPSSFQETIENQFDYICKQVIEDERKDYFKHLDRLSAKEVSFS
                     DIDNYLVNSFSTVDTYVTDFQLFKLFGLRINVESDLLSEALRHLSEKKRNIILLHYFM
                     DMSDVEIAELLNLNRSTVYRHRISGLAMIKEFMKECKE"
     gene            6283..6513
                     /locus_tag="ONT85_000358"
     CDS             6283..6513
                     /locus_tag="ONT85_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR2377358.1"
                     /translation="MKTAYPRVPFPLIVKATDGDVEAINQIVKHYRGYTSKRSLRRMT
                     DEYGNSHMVIDETLRGRMETKLISKILSFEIK"
     gene            7011..7211
                     /locus_tag="ONT85_000359"
     CDS             7011..7211
                     /locus_tag="ONT85_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008475635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HCR2377359.1"
                     /translation="MITVTKKDLVELGYGTSFSADIIKKAKELMITKGHIYYQSRKLD
                     RVPREAVEEILGIRFSNENRSE"
     gene            7238..8431
                     /locus_tag="ONT85_000360"
     CDS             7238..8431
                     /locus_tag="ONT85_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR2377360.1"
                     /translation="MVKDPIKKAKNGTYYFRANLGYDSKGKKIQKYRSGFTTKKEARE
                     EYSKLLLMKPEELSENKDKMTFKHYIFEIFLPWYKTQVKLRTYENRLPTIKKHFSYFD
                     KMAVSDIEPIDVQNWQLKLSKKCKSSYVRAVQGLFSVAMDRAIVLGITTTNPSKIIGN
                     VKKQKSKIEFWTKEEFEKVISLIYKEDYYQHFLFISLWFLFMTGMRIGEATAIQWEDI
                     DFDSGVLTINKTLYYKNQSNYRFVEPKTKASVRHIVLDKCTLTYLSEWKEVQQNLIQT
                     DFVMSYNGIPTQKHTLSYAIERYAKLAGIHRIRIHGLRHSHASLLISMGENPLIIKDR
                     LGHEDIETTLGTYGHLYPNSNFEVANKLNGAISFKEAKQNYDTSPKNQFTVDYLRNKE
                     MKKVQ"
     gene            complement(8499..10064)
                     /gene="guaA"
                     /locus_tag="ONT85_000361"
     CDS             complement(8499..10064)
                     /gene="guaA"
                     /locus_tag="ONT85_000361"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HCR2377361.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVDARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(10123..11046)
                     /locus_tag="ONT85_000362"
     CDS             complement(10123..11046)
                     /locus_tag="ONT85_000362"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HCR2377362.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            11242..11847
                     /locus_tag="ONT85_000363"
     CDS             11242..11847
                     /locus_tag="ONT85_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCR2377363.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            12155..13171
                     /locus_tag="ONT85_000364"
     CDS             12155..13171
                     /locus_tag="ONT85_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HCR2377364.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            13312..14613
                     /locus_tag="ONT85_000365"
     CDS             13312..14613
                     /locus_tag="ONT85_000365"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HCR2377365.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            14659..15321
                     /gene="deoC"
                     /locus_tag="ONT85_000366"
     CDS             14659..15321
                     /gene="deoC"
                     /locus_tag="ONT85_000366"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HCR2377366.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            15342..15740
                     /locus_tag="ONT85_000367"
     CDS             15342..15740
                     /locus_tag="ONT85_000367"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HCR2377367.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            15806..16888
                     /locus_tag="ONT85_000368"
     CDS             15806..16888
                     /locus_tag="ONT85_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR2377368.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            17100..18662
                     /locus_tag="ONT85_000369"
     CDS             17100..18662
                     /locus_tag="ONT85_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR2377369.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            18655..19788
                     /locus_tag="ONT85_000370"
     CDS             18655..19788
                     /locus_tag="ONT85_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR2377370.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            19788..20744
                     /locus_tag="ONT85_000371"
     CDS             19788..20744
                     /locus_tag="ONT85_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR2377371.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            20843..22009
                     /gene="deoB"
                     /locus_tag="ONT85_000372"
     CDS             20843..22009
                     /gene="deoB"
                     /locus_tag="ONT85_000372"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HCR2377372.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            22033..22851
                     /locus_tag="ONT85_000373"
     CDS             22033..22851
                     /locus_tag="ONT85_000373"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HCR2377373.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            22868..23575
                     /gene="deoD"
                     /locus_tag="ONT85_000374"
     CDS             22868..23575
                     /gene="deoD"
                     /locus_tag="ONT85_000374"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HCR2377374.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(23873..24559)
                     /gene="gpmA"
                     /locus_tag="ONT85_000375"
     CDS             complement(23873..24559)
                     /gene="gpmA"
                     /locus_tag="ONT85_000375"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HCR2377375.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(24731..25312)
                     /locus_tag="ONT85_000376"
     CDS             complement(24731..25312)
                     /locus_tag="ONT85_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR2377376.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(25445..26128)
                     /gene="rpiA"
                     /locus_tag="ONT85_000377"
     CDS             complement(25445..26128)
                     /gene="rpiA"
                     /locus_tag="ONT85_000377"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HCR2377377.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            26447..26860
                     /gene="rpsL"
                     /locus_tag="ONT85_000378"
     CDS             26447..26860
                     /gene="rpsL"
                     /locus_tag="ONT85_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HCR2377378.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            26944..27414
                     /gene="rpsG"
                     /locus_tag="ONT85_000379"
     CDS             26944..27414
                     /gene="rpsG"
                     /locus_tag="ONT85_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_466177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HCR2377379.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            27508..29595
                     /gene="fusA"
                     /locus_tag="ONT85_000380"
     CDS             27508..29595
                     /gene="fusA"
                     /locus_tag="ONT85_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HCR2377380.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            29767..30954
                     /gene="tuf"
                     /locus_tag="ONT85_000381"
     CDS             29767..30954
                     /gene="tuf"
                     /locus_tag="ONT85_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCR2377381.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            31361..31669
                     /gene="rpsJ"
                     /locus_tag="ONT85_000382"
     CDS             31361..31669
                     /gene="rpsJ"
                     /locus_tag="ONT85_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HCR2377382.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            31700..32329
                     /gene="rplC"
                     /locus_tag="ONT85_000383"
     CDS             31700..32329
                     /gene="rplC"
                     /locus_tag="ONT85_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HCR2377383.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            32357..32980
                     /gene="rplD"
                     /locus_tag="ONT85_000384"
     CDS             32357..32980
                     /gene="rplD"
                     /locus_tag="ONT85_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HCR2377384.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            32980..33270
                     /gene="rplW"
                     /locus_tag="ONT85_000385"
     CDS             32980..33270
                     /gene="rplW"
                     /locus_tag="ONT85_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HCR2377385.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            33309..34142
                     /gene="rplB"
                     /locus_tag="ONT85_000386"
     CDS             33309..34142
                     /gene="rplB"
                     /locus_tag="ONT85_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HCR2377386.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            34185..34463
                     /gene="rpsS"
                     /locus_tag="ONT85_000387"
     CDS             34185..34463
                     /gene="rpsS"
                     /locus_tag="ONT85_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HCR2377387.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            34486..34833
                     /gene="rplV"
                     /locus_tag="ONT85_000388"
     CDS             34486..34833
                     /gene="rplV"
                     /locus_tag="ONT85_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HCR2377388.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            34847..35503
                     /gene="rpsC"
                     /locus_tag="ONT85_000389"
     CDS             34847..35503
                     /gene="rpsC"
                     /locus_tag="ONT85_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HCR2377389.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            35506..35940
                     /gene="rplP"
                     /locus_tag="ONT85_000390"
     CDS             35506..35940
                     /gene="rplP"
                     /locus_tag="ONT85_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HCR2377390.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            35930..36118
                     /gene="rpmC"
                     /locus_tag="ONT85_000391"
     CDS             35930..36118
                     /gene="rpmC"
                     /locus_tag="ONT85_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HCR2377391.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            36143..36409
                     /gene="rpsQ"
                     /locus_tag="ONT85_000392"
     CDS             36143..36409
                     /gene="rpsQ"
                     /locus_tag="ONT85_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HCR2377392.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            36467..36835
                     /gene="rplN"
                     /locus_tag="ONT85_000393"
     CDS             36467..36835
                     /gene="rplN"
                     /locus_tag="ONT85_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HCR2377393.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            36872..37180
                     /gene="rplX"
                     /locus_tag="ONT85_000394"
     CDS             36872..37180
                     /gene="rplX"
                     /locus_tag="ONT85_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HCR2377394.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            37208..37747
                     /gene="rplE"
                     /locus_tag="ONT85_000395"
     CDS             37208..37747
                     /gene="rplE"
                     /locus_tag="ONT85_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_466143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HCR2377395.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            37766..37951
                     /locus_tag="ONT85_000396"
     CDS             37766..37951
                     /locus_tag="ONT85_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HCR2377396.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            37987..38385
                     /gene="rpsH"
                     /locus_tag="ONT85_000397"
     CDS             37987..38385
                     /gene="rpsH"
                     /locus_tag="ONT85_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HCR2377397.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            38417..38953
                     /gene="rplF"
                     /locus_tag="ONT85_000398"
     CDS             38417..38953
                     /gene="rplF"
                     /locus_tag="ONT85_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HCR2377398.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            39114..39470
                     /gene="rplR"
                     /locus_tag="ONT85_000399"
     CDS             39114..39470
                     /gene="rplR"
                     /locus_tag="ONT85_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HCR2377399.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            39491..39991
                     /gene="rpsE"
                     /locus_tag="ONT85_000400"
     CDS             39491..39991
                     /gene="rpsE"
                     /locus_tag="ONT85_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HCR2377400.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            40006..40185
                     /gene="rpmD"
                     /locus_tag="ONT85_000401"
     CDS             40006..40185
                     /gene="rpmD"
                     /locus_tag="ONT85_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HCR2377401.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            40229..40669
                     /gene="rplO"
                     /locus_tag="ONT85_000402"
     CDS             40229..40669
                     /gene="rplO"
                     /locus_tag="ONT85_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HCR2377402.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            40669..41964
                     /gene="secY"
                     /locus_tag="ONT85_000403"
     CDS             40669..41964
                     /gene="secY"
                     /locus_tag="ONT85_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HCR2377403.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            42026..42673
                     /locus_tag="ONT85_000404"
     CDS             42026..42673
                     /locus_tag="ONT85_000404"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HCR2377404.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            42864..43082
                     /gene="infA"
                     /locus_tag="ONT85_000405"
     CDS             42864..43082
                     /gene="infA"
                     /locus_tag="ONT85_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HCR2377405.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            43115..43231
                     /gene="rpmJ"
                     /locus_tag="ONT85_000406"
     CDS             43115..43231
                     /gene="rpmJ"
                     /locus_tag="ONT85_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HCR2377406.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            43249..43614
                     /gene="rpsM"
                     /locus_tag="ONT85_000407"
     CDS             43249..43614
                     /gene="rpsM"
                     /locus_tag="ONT85_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HCR2377407.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            43642..44031
                     /gene="rpsK"
                     /locus_tag="ONT85_000408"
     CDS             43642..44031
                     /gene="rpsK"
                     /locus_tag="ONT85_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HCR2377408.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            44111..45049
                     /locus_tag="ONT85_000409"
     CDS             44111..45049
                     /locus_tag="ONT85_000409"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HCR2377409.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            45077..45457
                     /gene="rplQ"
                     /locus_tag="ONT85_000410"
     CDS             45077..45457
                     /gene="rplQ"
                     /locus_tag="ONT85_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HCR2377410.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            45729..46568
                     /locus_tag="ONT85_000411"
     CDS             45729..46568
                     /locus_tag="ONT85_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HCR2377411.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMIFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            46544..47413
                     /locus_tag="ONT85_000412"
     CDS             46544..47413
                     /locus_tag="ONT85_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HCR2377412.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            47403..48200
                     /locus_tag="ONT85_000413"
     CDS             47403..48200
                     /locus_tag="ONT85_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HCR2377413.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            48403..49149
                     /gene="truA"
                     /locus_tag="ONT85_000414"
     CDS             48403..49149
                     /gene="truA"
                     /locus_tag="ONT85_000414"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HCR2377414.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKIRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            complement(49482..52151)
                     /locus_tag="ONT85_000415"
     CDS             complement(49482..52151)
                     /locus_tag="ONT85_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HCR2377415.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDGGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEISFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEQPRNTHRRRRRK"
     gene            complement(52389..52577)
                     /locus_tag="ONT85_000416"
     CDS             complement(52389..52577)
                     /locus_tag="ONT85_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HCR2377416.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            52847..53209
                     /locus_tag="ONT85_000417"
     CDS             52847..53209
                     /locus_tag="ONT85_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HCR2377417.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            53261..54943
                     /locus_tag="ONT85_000418"
     CDS             53261..54943
                     /locus_tag="ONT85_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HCR2377418.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKEIPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            55060..57096
                     /gene="recG"
                     /locus_tag="ONT85_000419"
     CDS             55060..57096
                     /gene="recG"
                     /locus_tag="ONT85_000419"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HCR2377419.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKQFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LITGSTKAKERRELLEKLEQGEINVVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            57145..58146
                     /gene="plsX"
                     /locus_tag="ONT85_000420"
     CDS             57145..58146
                     /gene="plsX"
                     /locus_tag="ONT85_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HCR2377420.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            58268..58510
                     /gene="acpP"
                     /locus_tag="ONT85_000421"
     CDS             58268..58510
                     /gene="acpP"
                     /locus_tag="ONT85_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HCR2377421.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
     gene            58778..59782
                     /locus_tag="ONT85_000422"
     CDS             58778..59782
                     /locus_tag="ONT85_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR2377422.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            59783..60727
                     /locus_tag="ONT85_000423"
     CDS             59783..60727
                     /locus_tag="ONT85_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR2377423.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            60727..61689
                     /locus_tag="ONT85_000424"
     CDS             60727..61689
                     /locus_tag="ONT85_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR2377424.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            61716..62630
                     /locus_tag="ONT85_000425"
     CDS             61716..62630
                     /locus_tag="ONT85_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR2377425.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            62656..64437
                     /locus_tag="ONT85_000426"
     CDS             62656..64437
                     /locus_tag="ONT85_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR2377426.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            64785..65471
                     /locus_tag="ONT85_000427"
     CDS             64785..65471
                     /locus_tag="ONT85_000427"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HCR2377427.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            65491..69072
                     /gene="smc"
                     /locus_tag="ONT85_000428"
     CDS             65491..69072
                     /gene="smc"
                     /locus_tag="ONT85_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HCR2377428.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIETALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            69081..69890
                     /locus_tag="ONT85_000429"
     CDS             69081..69890
                     /locus_tag="ONT85_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCR2377429.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            69901..70899
                     /gene="ftsY"
                     /locus_tag="ONT85_000430"
     CDS             69901..70899
                     /gene="ftsY"
                     /locus_tag="ONT85_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HCR2377430.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            71086..73353
                     /gene="gshAB"
                     /locus_tag="ONT85_000431"
     CDS             71086..73353
                     /gene="gshAB"
                     /locus_tag="ONT85_000431"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HCR2377431.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(73442..73894)
                     /locus_tag="ONT85_000432"
     CDS             complement(73442..73894)
                     /locus_tag="ONT85_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HCR2377432.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            74044..74994
                     /locus_tag="ONT85_000433"
     CDS             74044..74994
                     /locus_tag="ONT85_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HCR2377433.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            74987..75946
                     /locus_tag="ONT85_000434"
     CDS             74987..75946
                     /locus_tag="ONT85_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HCR2377434.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            75943..76698
                     /locus_tag="ONT85_000435"
     CDS             75943..76698
                     /locus_tag="ONT85_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR2377435.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            76721..77674
                     /locus_tag="ONT85_000436"
     CDS             76721..77674
                     /locus_tag="ONT85_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR2377436.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(78318..78614)
                     /locus_tag="ONT85_000437"
     CDS             complement(78318..78614)
                     /locus_tag="ONT85_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HCR2377437.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            78969..79325
                     /locus_tag="ONT85_000438"
     CDS             78969..79325
                     /locus_tag="ONT85_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HCR2377438.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            79335..80234
                     /locus_tag="ONT85_000439"
     CDS             79335..80234
                     /locus_tag="ONT85_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HCR2377439.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
BASE COUNT        26317 a        14464 c        17098 g        22557 t
ORIGIN      
        1 gtccggtaaa tcattagaag cattggaact cgaacaagag gaaagcataa gatttcagaa
       61 ttgttctctg ttccctcttt atcatggaag tgcaaaaagt aatataggga ttgataacct
      121 tatagaagtt attactaata aattttattc atcaacacat cgaggtccgt ctgaactttg
      181 cggaaatgtt ttcaaaattg aatatacaaa aaaaagacaa cgtcttgcat attaagtagt
      241 tcagttttgg agtacaaaaa gtgaaaatgt aaacctaaat gagtatcaaa ttgcaactga
      301 atttgatact cgttttttat tgttaaatca acgtttgttg ttaatttaca ccacaaaatg
      361 taagttaaaa tgtatataaa aatgcgaacg tgaaataatt attgtaggaa tgcaaattgg
      421 tgtgttaaaa tggttacaat ttactaagca aaaaggggtg cattttccta tgcaatttct
      481 tgagccaaaa aataagaacg ctaagtctgt ggattgggaa atatcagagc aagtaagggt
      541 gatagttaag caatacgctg aatatgcaga gcgtactgaa agcgaagcag tggatgaatt
      601 cttattgaac attttagacg ataagaagtt tattgaatgg attgccaaca agcgaagtaa
      661 taagcggatt gttgaaaaaa tgggtatcaa agatagagtg ggttgataga caatgaccaa
      721 gcttaaaaga ttagccacga aggaagatga aattgtagat gttaaaattc cgatttcaaa
      781 cgaagaatta aaagataggg ctaaacaata tgatttatta actccaaaac gatttgcaac
      841 aaggtataac aaaatgttgt tcttgccaac gtcttttaaa tggaacggtt ctgaatatcc
      901 cattcaatat aattactgca tcaatccgtt ttgttgtaat tttgggaaag aacagcacaa
      961 attcaaagat gtaaaaggga aaccaagtcg atataaaatg actggtagtt ccaaagataa
     1021 aggtcataaa ggtatgtact gcaatgacaa tcctattggc agaggtgtat cgcaaaactg
     1081 tacagtaacc cctttatcta attggtctgt tgttgaagaa attaaacgcc ttatagaaat
     1141 caatagcatc caagatgttg agccagatta tcaatttcat aaagaaggct gctcggaaga
     1201 agaatcaacc ccattcaatg agccgaagca attttacaaa agggggaaaa gtaggggcaa
     1261 atctcaacgc tatcaatgta aggcgtgtaa gaagttcact aatgttctac caaaaagaga
     1321 agaaaccact acttatcacc aacaaaagaa caccattctt ccaatgttcg ctaagatggt
     1381 tgttggaagg gtttcagtca gtcgtacttg tgatatttta ggaattggag ttggaaccta
     1441 ctatcataag ctggagtggc tgtataggcg ttgtttggag tttttagaac gatatgagac
     1501 acaacccctt caaacaaaga aatttaacga gatgtggctt aatacggaca agatgcacta
     1561 ctacctaaat aacgtccgta agaaaggaca aggttcaaag aaatatacgg ggtttgaaga
     1621 ccttaatatg caaacgtata tagttgtatc tgctgaggtc ttatcaagat atgtgttccg
     1681 ttctgatgtt gcctatgatt ggaatatttc aatggatgaa ttaaatgaag atacaagaaa
     1741 attcaaagaa gaccatctca atacttttag tcgtaaaaat gataggttag attggtctta
     1801 ttatccacaa gaaccatccg ctaacgatag cgaaaatagg aatgcttatc ttcacgaatt
     1861 agggaaaatt actaatagga gtagatttgt cgatggttta aatgttgatg ctccttatac
     1921 tacaactgct cactattggt taattaaaca aatggttaat gccgacgaat ggcgaatgat
     1981 tagtgatgat gacttctcta ttagaaacgc cttttataga gtattcacga aagaattaag
     2041 gttgagcgat gcacatcatt ttatttgcca agttaacaag acgaaaagca gaaaacaatg
     2101 cttaaaagaa ttcggacaag caaaagcaga actgctcgat tggggagata tcagaggatt
     2161 caaaacaaag tttctacgga ctttggcatc tcattatcta actgaattac taacaagtca
     2221 tcaattccac gaagaggcta ttagtaaaga tggagaaaga tatcgcaaat atgccgataa
     2281 ccctatcaaa catccattag ccactaagga taaaggattt tattcagttg attgtagaac
     2341 ggatttatct gctctggaac caaacgaaat cgcaaagatg ctattaaatg ttaatgacca
     2401 ttcgaccaat agctttattc aacagataag acgttatata tcatctcttg aaagaccatt
     2461 aacaacagct cgtggtgata agaagagcta tatttacgct aacttcaatc cgaagtatgc
     2521 tcaatttgcg attacaattc taagaactta ctacaacttt tgtcgccctt ttaaatcagc
     2581 ggataaaaag gttttgacac ctgctcaaag attagggatt acagataaac aatttgattg
     2641 gaaagatata atttatttca aatgacaaaa agagcttgga gtaatcccaa gctcttgtgc
     2701 tttatcttta ctgttcttct tcaattaacg cacccgttaa ctcaataagt tttttctttc
     2761 atatacatga aaatagtagt tatacggatt tttatcattc tttatccctt tttgggcaaa
     2821 tacctcattc cattcctcat aattcacttc tggaaaaaaa gtatctcctt cgaattcatg
     2881 atgtattttt gtgatgtaca ttttctcaac ataagggaaa aacaaattat aaatctgttc
     2941 tcctccgaaa ataaaaattt cttcttcgtt tttacataac tcaaaaacat cttctattga
     3001 atgaacaatt tcacaaccat taaaggtaaa ccccttatct ctcgtcagaa taatatttct
     3061 tctgtcaggt aaggctcttc cgattgattc aaggttcttc ctacctaata ttatcggatg
     3121 tccctttgta gtatttttaa catattccca gtccttggga atcctccaag gaatgtcatt
     3181 ctctttgcca atcactctat tcttatccat cgcagcaatc aaagaaactt tcatttataa
     3241 cacagccttt ttaactaatt taccatctaa tttcttattt ctatcaaaaa accttttctc
     3301 attatcctgc ccgttagttg aataagcaat tttttctata agttatatta tcataatacc
     3361 acaaaatcac aaattttcat tcaattatca ttttcaattg cattttataa atcattttgt
     3421 atgtaatttg gtatgtaagt taaaatgaaa aaagtagtcc attttgaagt acattttgtc
     3481 attttgtact ccaaaactga actacttact tgcatatata cgcctttata gtggagtact
     3541 acatttacga gattcggtta gagtatcaga aaaagaaaaa ataaaagtta cagaaatgta
     3601 tacttcaata aatggtgaat tatgtaagat tgatagagct tattctggag aaattgttat
     3661 tttgcaaaat gagtttttga agttaaatag tgttcttgga gatacaaaac tattgccaca
     3721 gagaaaaaag attgaaaatc cgcaccctct actacaaaca actgttgaac cgagtaaacc
     3781 tgaacagaga gaaatgttgc ttgatgccct tttggaaatc tcagatagtg atccgcttct
     3841 acgatattac gtggattcta cgacacatga aattatactt tctttcttag ggaaagtaca
     3901 aatggaagtg attagtgcac tgttgcaaga aaagtatcat gtggagatag aactaaaaga
     3961 gcctacagtc atttatatgg agagaccgtt aaaaaatgca gaatatacca ttcacatcga
     4021 agtgccgcca aatcctttct gggcttccat tggtttatct gtatcaccgc ttccgttggg
     4081 aagtggaatg cagtatgaga gctcggtttc tcttggatac ttaaatcaat catttcaaaa
     4141 tgcagttatg gaagggatac gctatggttg cgaacaagga ttatatggtt ggaatgtgac
     4201 ggattgtaaa atctgtttta agtacggttt atactatagc cctgttagta ctccagcaga
     4261 ttttcggatg cttactccta ttgtactgga gcaagccttt agaaaagctg gaacagaatt
     4321 gttagagcca tatcttagtt ttaaagttta tgcaccacag gaatatcttt cacgggcata
     4381 taacgatgct cccaaatatt gtgcaaatat cgtaaatact caactgaaaa ataatgaggt
     4441 cattattatt ggagaaattc ctgctcgatg tattcaagat tatcgcaatg atttaacttt
     4501 ttttacaaat gggcttagtg tttgtttagc agagctaaaa ggatatcagg ttaccactgg
     4561 cgaacctgtt tgccagaccc gtcgtctaaa tagtcggata gataaagtaa gatatatgtt
     4621 caataaaata acttagtgcg ttttatgttg ttatataaat atggtttctt attaaataag
     4681 atgaaatatt ctttaatata gatttgaatt aaagtggaaa ggaggagatt gttattataa
     4741 actacaagtg gatattgtgt cctatttgtg gaaataaaac aagactacga atacgagtgg
     4801 atactatact taaaaatttc cctttatact gccccaaatg taagaacgaa actttaatta
     4861 atgttcaaaa aatgaatata ataacaatca aagagccaga cgccaagacg cagagccgat
     4921 aatttgagaa atgaaactct catcttatcg gctctttttg tttatctgaa ttttactgac
     4981 tagccttcaa tatttcttgc ttcattgata ccttttgcta gtgcttccat taaggatagt
     5041 tctttgtctg taaagctatc catgtatttt tctacctgta accttcgagt gcttttaatc
     5101 aaattatctg taggtaagaa aaattcatca atagatacat ggagtaatga tacaagatca
     5161 tacagcactt gtgtacttgg gtgttgtcct ttgttttcta tatttgttaa ataacgtggg
     5221 tcaatttcaa tcattgttcc cacttgttca cgagtgagac cccgtttcat tcgagcctct
     5281 ttaatcgcta aaccaacggc tctaaaatcg tatttatctt cttttttacg catactaaat
     5341 cacctctata cattttactg ttcctatata accataaaca ggtatagaaa aacatgttat
     5401 atagtttatg agttcctata gaacactgtg gaatttatta atgcgatgaa tccaatttca
     5461 aatagaaagg tcgctgattt tatttaccta tacaacttta taaaatttag tcatactcct
     5521 agaaagtaga taaaattggc gacttaatgg agggaaaagt cgccatgaga aagataatct
     5581 gtcatctagt tctcttaatt aagttcttat caaaaaaact tatccacctg tactggatag
     5641 atatgaccat caatgtggtt ttgcagatta catagataga aaaatcaaag catatgaaca
     5701 gagaaaacaa tctgtttatg tgcttttttg gtcacttaat aaatatcttt taggatttct
     5761 cttcatttat gcaacaaatc ccccgttcgt gtcggggttt agtatgaagg gagataaaga
     5821 agcacatgcc actccctttc acgacagaaa gggggtgaaa agcatgaaac catcttcttt
     5881 tcaagaaacc atagaaaatc agtttgacta tatctgcaaa caagttattg aagatgaacg
     5941 aaaagattac ttcaaacatc tagacagact atcagcaaaa gaagtctcgt tttctgatat
     6001 agataactac cttgtcaact ctttttctac tgttgatacg tacgtcactg atttccaact
     6061 atttaaatta tttggattaa gaatcaatgt tgaaagtgat ttattaagtg aagccctgcg
     6121 tcatctttca gagaaaaaac ggaacattat tttacttcat tactttatgg atatgagtga
     6181 tgtggagatt gcggagttac taaatttgaa tcgttcgact gtttatcgtc atcgaataag
     6241 tggactagcc atgattaaag aattcatgaa ggagtgtaaa gaatgaaaac agcttatcca
     6301 agggttccct tccccctcat tgtaaaggcg acagatggcg acgtggaagc aattaatcag
     6361 attgttaaac attatcgagg gtatacctca aaacgttcat tacgtcgcat gacagacgaa
     6421 tatggaaatt ctcatatggt tattgatgaa accctacgtg gcagaatgga aacgaaactc
     6481 atctctaaga ttttatcgtt cgaaattaaa tagtcccatt ttttcgtgga agcgtgtcga
     6541 atcgtatcca cgcttcccga tttgggaggt ttgttattcc atcaaagcat attgtgtgtt
     6601 cagtaggttt tgataggcta acaagccaaa cgttctttga caaccaaata aaggcaatcg
     6661 aatacgtttc ggtaagaaaa gagctgatga actaacacgc catgacctac aaaattagtg
     6721 agcgattcat gttagtaatt cgaaagtaat ctttagcagg attgctgcca tgactttctt
     6781 atcgtgataa tgatactccc gtatagccat agtccgagcg ttagaagcgt cacaggcaat
     6841 gggtacggct acatgagaac catgcagaag tgaaattcct gtgagctttg ctagagctgt
     6901 tcgattgcta agatattctt ctcttgtcag tgtattatta taaaactatt tgatataatt
     6961 ataggtgaat attggagttt tcctagccta taattgggaa aggagtaata atgataacgg
     7021 tcacaaaaaa ggatttagtt gagctaggat atggaacttc tttttcagca gacatcataa
     7081 aaaaagcaaa agaattgatg attacaaaag ggcatatcta ctatcaatct cgaaagctag
     7141 atagagtccc tcgtgaagct gttgaagaaa ttctaggaat tcggttttca aatgaaaaca
     7201 ggtctgagta gtttttgcac agtaaggagt aaaaactatg gttaaagacc caataaaaaa
     7261 agcaaagaat ggaacctatt attttcgtgc aaatctaggt tatgattcta aagggaaaaa
     7321 aattcaaaaa tatcgtagtg gattcactac taaaaaagaa gcaagagaag aatattcgaa
     7381 actgctattg atgaaaccag aagaattaag tgagaataag gataaaatga cgtttaaaca
     7441 ttatatcttt gaaattttcc ttccatggta taagacacag gtaaagctca gaacatatga
     7501 aaatcgctta ccaactatta agaagcattt ctcttatttt gataaaatgg ctgtttccga
     7561 tattgagcct attgatgtgc aaaattggca actaaaatta tcaaagaagt gtaagtcttc
     7621 ttatgtaaga gctgttcaag gactattttc agttgctatg gatcgagcaa ttgtccttgg
     7681 gataaccacc acaaatccat ctaagattat tggcaatgtg aaaaagcaaa agtctaaaat
     7741 tgaattttgg acaaaagaag aatttgaaaa agtaatctct ctaatttaca aagaagatta
     7801 ctatcaacac tttttattta tctctctgtg gtttttattt atgactggaa tgcgaattgg
     7861 tgaagctact gccattcaat gggaagatat tgattttgac tcaggagtac taaccatcaa
     7921 caaaacactc tattacaaga atcaatcgaa ttatagattt gttgaaccaa aaacaaaggc
     7981 gagcgttcgt catattgtat tagataaatg cacattaact tatttatccg aatggaagga
     8041 agtacaacaa aatctaattc agacagactt tgtaatgagc tataacggta ttcctaccca
     8101 aaaacataca ctatcatatg ctattgaacg ctatgctaag ctggctggta tccatcgaat
     8161 cagaatccat gggttacggc attctcatgc ttccttgtta atcagtatgg gagaaaatcc
     8221 acttatcatt aaagatcgtc taggacacga agatattgag acgactttag gaacgtatgg
     8281 acatctttat cctaacagta atttcgaggt agctaacaaa ttaaatggag ccatttcctt
     8341 taaagaagca aaacaaaatt atgatacttc accaaagaat caatttactg tagactactt
     8401 gcgaaacaaa gaaatgaaaa aagtgcaata aaagtgcagt aaaaagataa gaagagccag
     8461 aaacccttgg tataagagga tttctagctc tcctacaact attcccactc cactgttgct
     8521 ggtggtttag aagtgatgtc atacacgata cggttgacgt gggctacttc gtttacgata
     8581 cgtacagaga ttttttgcaa gacttcccat gggatacggg caaagtcagc ggtcattccg
     8641 tcgattgaag ttacagcacg gataccgact gtgtagtcgt aagtacgtcc gtcacccata
     8701 acaccgacag agcggatccc tggaagaaca gtgaaatatt gccagatatc acggtcaagt
     8761 ccagcagctg cgatttcttc acgcaagata gcatcagatt cgcgtacgat ttctagcttg
     8821 tcttcagtga tttcgccaag aacacgaata ccaagaccag gaccagggaa tggctgacgc
     8881 caaacgattg aatcaggcat gcctaactga gtccctaatt cacggacttc gtctttgaat
     8941 aatgtgttta atggttcgat caattcaaat tgcatatctt ctggcagtcc acctacatta
     9001 tggtgagatt tgatcgtctg tgctgtttct gtaccagatt cgataacgtc agtatacaac
     9061 gttccttgtg ctaggaaaga gacgccttct tcaccagcaa gttttgttgc ttcgtcatca
     9121 aaaacatata cgaattcatt cccaatgatt ttacgttttt gttctgggtc agatacacct
     9181 ttcaacttgt ctaagaagcg atctcttgca tcgactttga tgatgttcaa gccgaatttt
     9241 ccgcctagac tttccattac ttgttccgct tcgcctttgc gtagcagacc gtggtcaaca
     9301 aagatagatg tcaattggtc accgatcgct ttttgtaaaa gaaccccaac gacactagaa
     9361 tcaacgccac ctgaaagacc aagcaagact tttttgtcac caacttgttc acggattttt
     9421 tctacttgca tatcgataaa gttatccatt gtccagttac cttcacagtg acaaacatca
     9481 aaggcaaagt ggcgcagcaa ttcgattccg tattcggaat gacgtacttc tgcatggaat
     9541 tggataccat aaaaattacg tgttgtgtct tggatagaag caatcggaca atctttgctt
     9601 gttccgactg tttcaaagcc ttcaggaact ttagttacca agtcaccatg gctcatccat
     9661 actgtttgtt gttttggtgt ttctttaaac aagacagctt gatcgcttgt tacttctaac
     9721 aaggctttcc catattcgcg gtttttagca ggctctacgg aaccgccaag tttgtacgtg
     9781 atcaattgca taccatagca aatccctaat acaggaatac ctaaattgaa gatctcagga
     9841 tcgatcccaa atgcattctc atcatataca ctgtttggtc cgccagaaag aatgattccc
     9901 tttggattca tcgcttttac ttcttctgca gtaatgcggt ggctcaataa ttcggaaaag
     9961 actccaagat cacgaatacg tcttgtgatc aattggttat actgactacc gtagtctaat
    10021 acgataatct tttcaacgtt cgtcatatcg gcaacgtttg tcaaatttat tacccctatc
    10081 cctttaaatt ttatctcaaa atagtcaaac tattttaaaa tgctaatatt ttcgcatgaa
    10141 aatcgaatcg atgatatgat tttcagtttt atggagaatt atatcggctc ttcctcgtgt
    10201 aggcaagata tattcctgta aatttttcaa attcacattt ttccatacat cttttgccat
    10261 tttaaaggct tcttcacgat ctccgatagc aaaacgataa taataattct ctggattttg
    10321 gaaagccgta tccagtaacg caccaaaacg atccaaatac catttttcga tcagcacggg
    10381 atcggcatcg acatagacag aaaaatcaaa aaagtcgctg acgtagattt gctgattcgc
    10441 aggtaattga agcgtattga tcccctcaac gatcaagata tcaggctgct ggatcaattc
    10501 gtacttgtct ggaatgatat catacacatc atgtgagtaa acaggtactt ttatctcttc
    10561 ctgaccagat tttacacggt tcaagaaatc gatcagcatc tccatatcat aactttcagg
    10621 aaatccttta cggtccatga ttccacgctc ttttaatata cgatttggat aaagaaatcc
    10681 gtcagtcgtg atcagctgaa cgtttcttcg tttgaaggta cgagacaaaa tcgtctgcag
    10741 cagtctggct gttgtacttt ttccaacggc tacacttccg gcgatcccga taataaaagg
    10801 cggtacttct acgtatttat gaagaaataa acctttactc aagaacaaag attcaaattc
    10861 tttcatataa agataaatca gatgagcaag aggtacatag atatctcgaa catcctgcat
    10921 ggaaatgcgg tcattcaaac ttttgatatt atctagttca tcttgtgtca aaggtgcttg
    10981 cccattacga taaaattctc gccattcttc tcttgccaca cggtagtagt tcattggatc
    11041 attcatatta gcctcctgcg ttctcccatc aatggaacta ttttaacaca aaataaacgc
    11101 aaaccccacc acttaagaga gattgccgat ctttttgtat aatctttata atagaaagaa
    11161 acggagtctt attcttgact tgcgcacgga tacataaaag gatagactgt tgtcatgaaa
    11221 gaaatgagaa ggatggaaga catgacaaat cattactatt ctgaacgccc agatacggcg
    11281 cacgattttg aacaatgggc ttttgagtta aaaggcagaa cctttcaatt tgtaacagat
    11341 tctggtgtat tttcacgtga aacagtggat tatggttcac gtgtcctgat tgatgctttc
    11401 gattgggaag agttacctga tgaaggaaag atcttagatg tgggatgcgg gtatggaccg
    11461 attgggttag ctttggcatt tgcgacacag cggtttgttg aaatggtcga cattaatgaa
    11521 cgagcggtaa gtctagcgca aggaaacgca aagcgtaacc aaatcgaaca tgttgatatc
    11581 catcaatcga atatctatga agcagttcat gaagaaacct atgcagctat cgtaagcaat
    11641 ccgccgatcc gtgctggaaa aaaagttgta catgaaattt tgacaggcgc atatcctcgg
    11701 ttgaagaagg gcggtacact gacaattgtg atccagaaga aacaaggagc gccgagtgcg
    11761 caaaagaaaa tggaagaaac atttggtaat gcagaaatcg tcacaaaaga taaaggatat
    11821 tatatcatca aaagtgtgaa agaataacta gctgaaaaat agtggagcga cgaaagagtg
    11881 gaactgggcg tgcatgtacg ggtacgtatc agttcttttt ttgttgagaa gtttttaaaa
    11941 acaaagaact attttttctt caaaaagaaa tagtaggaac agagtcgttg ttttttacga
    12001 taaaataaat aagatacaga acttgtatag aaggctgcca agtcggtaga taaaggaggt
    12061 ttttatcaaa aaacatactt tccttttttc tctattcttt tcttgggggt tttggtacac
    12121 tgatagggaa tttagataga agggagccaa taggatgaag ctgaccatca gagatatcgc
    12181 agaaatggca ggcgtatcag taacaactgt ctcacagatt ttgaataaca aaggaagcag
    12241 atttagtgat aagacaagaa aaaaagtgtt gtcgatcgtg aatgaacatc attataagcc
    12301 cgattatttc gcttcaaatg tgatcaatcg acattcaaag accatcggaa tgatcgtccc
    12361 tgatgtcact gatttcttct tctccaaact gatcgaaggt gtagagagct atttgaatcc
    12421 tttgggctat gtgatcatgt tatgcaattc tcgtcatagc caagaaaatg agatcaagta
    12481 tttacaagag ctttcccacc gctcagtgga cggaatcttg ctggccacac caaatatttt
    12541 acctgagaaa tatgcattag acagcggttt ttatcaaaaa atgccaatca ttttaatcga
    12601 tcgcggattg aatcgtcggg ataacggacg gcttatcgta aaggaatatg agggcgccta
    12661 tcaagctgtt tcgctgttta ttgaaaatgg ccatacgaag atcggcatgc tgaaggaaac
    12721 cactggatac tatcaattag aagaacgttt caacggatat cgtcatgcgc tgaaagatca
    12781 tggcctgcct tttaatggga aatttgttga acatggagag ttgactgtcc aaggtggata
    12841 tactgcatca aaaaaattac tcaaacataa agatatcacg gctatttttt gcgggaatga
    12901 tgccatggcg atcggatgct atcaggcgat cgatgaattg ggcaaaaaaa tcccagaaga
    12961 tatctcagtc ataggattcg atggattaaa gctgtcagaa tacatgattc cgcaattgac
    13021 gacagtgcag cagccaagtt ttgatattgg attttatgct gcacgtttcc tgatcgatac
    13081 gatcgaattt cctcaaagaa aagttccgaa caaagtgttc gaaacaaaat taattattcg
    13141 tgagagcgta aaagtattga caaagggcta actcggcggt atattgctta tgtaagcatt
    13201 tacaaaggct agttagtgct ttttttattt ttagtaaacc agtttactaa agtggtttac
    13261 ctttcctaaa aataatgtat aataccgaat gaagacaagg aggatcaggc aatgcgaatg
    13321 gtggatctga ttgaaaaaaa gagggatgga aacgagctgt cgaaagagga aatcgagtac
    13381 atcgtcacga actatacgaa tggaaagatt ccagattatc aagtcagtgc attattgatg
    13441 gcaatttttt atcaagacat gacaaatgaa gaaatcacga acttaacatt ggcgattgct
    13501 aattctggtg atgtcatcga tttgagttct cttgaaggaa taaaggtaga taagcattcg
    13561 acaggtggcg ttggtgacac gaccacattg attttagccc ccctagtagc tagtgtcgga
    13621 gtgacagtgg ctaaaatgtc tggacgcggt ctaggttaca caggtggtac actagacaag
    13681 ctagaagcaa ttcccggatt tcagatcgag ctttcggatg aagaatttgt acgcattgtt
    13741 aatgaaagta aagtagcggt aattggtcaa tcaggaaact tggcaccggc ggacaaaaaa
    13801 ttgtatgcct tgcgtgacgt cacagcgacc gttgattctc ttccgctgat tgccagttcg
    13861 attatgagta aaaaaatcgc ggcaggagca gatgcaatcg ttttggatgt gaccactgga
    13921 gacggtgctt tcatgaagaa catcgaagat gcaagacgtc tggcaaagac catgacgagc
    13981 attgggaaac tggctaaccg agaaacagtg gcggttatct cagatatgtc agaacctctt
    14041 ggcgaagcta tcggaaatag tctagaagtt gtcgaggcaa tcgaaacatt acaaggaaat
    14101 ggtccagaag atctagtaga aatgtgctac gccttaggaa gccaaatggt tgttcttgct
    14161 ggcaaagcta agacaaccga tgaagcaaga acgttgcttc aagaagctct ggaatcaggt
    14221 aaagcgttag caaaattcaa agaaatgatc caaaatcaag ggggagaccc agcgatcgtg
    14281 gaacatccag aacgaatttt gactgcacgc tacacaatgg agcttcctgc taaacaaagc
    14341 ggagttgtgt caaaaatcgt ggcaaatgaa ttaggaattg ctgcaatgat gctaggtgct
    14401 gggcgtaaaa ccaaagaaga agatattgat cacgctgttg gattgaaact gcataaaaaa
    14461 attggtgaca ctgtgacaaa aggtgagtca ctattgacga tctatagtaa cgacgaagaa
    14521 atcacttctg tcattgaatt attatataaa aatattgaaa ttggtgaatc ggcaatgaag
    14581 ccaacgttga ttcatgacat catcacagaa taaaggaaaa aagcggactt taccgtttta
    14641 aagaaaaggg gaaaagaaat ggaactaaac cgaatgatcg accatactat tttgaaagca
    14701 gacgcatcta aagaagctgt gatgcaaatt atcgaagaag caaaaaaata ccatttttat
    14761 tctgtctgca tcaatccagc atgggttgcg ctagcgaaag aacaattaca aggtgagcca
    14821 gtagccgtat gtacagtaat cgggtttcct ctaggagcaa acacacccga gacaaaagca
    14881 tttgaaacaa cagatgccat caataacggt gcagatgaag tcgatatggt catcaatatc
    14941 ggtgctttga aatcaaagga tgatcaacaa gtccaaaaag atatcgaagc agttgttgaa
    15001 gcagcaaaag atcgtgcgct tgtcaaagtt attattgaaa catcgttatt agatagagaa
    15061 gaaatcatcc gagcttgcga aatagcaaaa gctgcaggcg ctgattttgt caaaacatca
    15121 actggtttct caacaggcgg agcaaaggta gaggatgtcc gtttgatgcg tgaaactgta
    15181 ggaccagaaa tgggtgtcaa agcttccggc ggtatccata acgaagaaga agcaatggca
    15241 atgatcgaag ctggtgctac aagaatcgga acaagcgcag gagtagcaat cgtatctggt
    15301 tcaactggtg aaggctatta attttttcaa aggagaagct catggcagaa ctcaaacaag
    15361 aatggatcaa cacagctatt gaagcgttag ataaagccta tgttccttat tcccattttc
    15421 cggtaggcgc ttgtctcgta acggaatcag gcaagattta tcaaggcatc aacattgaga
    15481 atgcatcatt tggattaacg aattgtgccg agcgcacagc ttttttcaaa gcagtatctg
    15541 aaggagaaaa aacatttacg catctagtcg tagcggggca cacccccgat cctatctctc
    15601 cttgtggtgc atgtcgacaa gtaatggcag aattctgttc ccctgatatg ccggttacgc
    15661 tagtaggaga caatggtgtg acgaaggcaa cgaccgttcg tgcactgctg ccttatgcat
    15721 ttactgaaaa agatctataa ctcatgttca ttcagtcagc tgactgttga aaaataaaac
    15781 aattatttta ggaggcttta cagaaatgaa aaaagcaaaa ttatttggtt taggtgctgt
    15841 cgcactagca gcagggttat ttttaggtgc atgcggcaac aacggctcta ctgattcatc
    15901 cggtggcaaa agctcatcag acacaacaac tgcagcattg atcacagaca caggtggtgt
    15961 agacgaccgt tcattcaacc agtcagcttg ggaaggacta gaaaaatggg ggaaagatca
    16021 aggactttca cgaggaaatg atggcttcca atatttccaa tcttcaaatg aatcagatta
    16081 catcccaaat attgaccaag cattgaatgc tggattcaaa acgatttttg gtattggtta
    16141 taaactaaaa ccagcgatcc aagaacaagc aacaaacaat acaggtacga actttgttat
    16201 tatcgatgat gtcatcgatg gattagataa cgttgtatct gcaactttca aagacaacga
    16261 agcttcttat cttgcaggtg ttgcagcagc ttatacgact gaaacaaacg tagtcggctt
    16321 tatcggcggt gtaaaaggtg aagtaatcga tcgttttgat gctggattca aagccggtgt
    16381 tgatgcaggt gcgaaagaat taggaaaaga aatcaaagta ttgaatcaat atgctggtga
    16441 cttctctgct ccagataaag gacgttcaat tgctcaaggt atgtatgcac aaaacgcaga
    16501 tatcatcttc catgcttcag gcggtacagg taacggtgta ttccaagaag caaaatcatt
    16561 gaacgaatca ggcgacaaaa aagtttgggt tatcggtgtt gaccgcgacc aatcagacga
    16621 aggcgaatac acattaaacg gcgaaaagaa aaacttcaca ttgacatcta cactgaaagc
    16681 agttggaaca gtagtagaag atttggcaca aaaatcagcg gacggtaaat tccctggcgg
    16741 cgaacacact gtttatggat tgaaagaaga cggtgttggt ctaacagaag gacaactttc
    16801 tgacgaagcg aaaaaagcag ttgatgaagc aaaagaaaaa atcatttcag gtgacgtaaa
    16861 agttccagaa actcctgaag aaaactaaaa ccatttctat agccttgtta gacacatgtt
    16921 tgacaaggct atggtttgat tagaacaaga attgttcatt tatccaagct gccttgtgac
    16981 agtttggata agggaacaac tccctgaagt aatgtttgtt tcaaaagaag aacgctcact
    17041 aagcgtgaat cttagtcaat cgttgcccta gatagaaatc gttaggatgt gaaaaaaagg
    17101 tggttcaaga aaatattgtc atagaaatgc agaatattac caaagaattt ggaacattca
    17161 aggcaaatga caacatcaat ttacaagtaa aggcaggaga aattcatgcg ttgcttggag
    17221 aaaacggtgc tggcaaatct acattgatga acgtgctttc cggattatta gagccgacat
    17281 cagggaaaat tttgatgcgt gggaaagaag taaagatcac aagtccgacc aaagccaacc
    17341 aattagggat tgggatggtc catcagcact ttatgcttgt tgatgccttt actgtaacag
    17401 aaaacatcgt gttgggaagc gagcctagtc gcgcagggat gcttgaccat aaaaaagcac
    17461 gaaaagagat ccaaaaagtt tctgaacagt atggattgtc agtcaaccca gatgcttacg
    17521 ttcgtgatat ttcagttgga atggaacaac gggtagaaat tttaaaaaca ctttaccgag
    17581 gagcagatgt actgattttt gatgagccga cagctgtatt gacccctcaa gaaattgatg
    17641 aattaatcgt gatcatgaag gaattagtca aagaaggcaa gtcgatcatt ttgattacgc
    17701 ataagttaga tgaaatcaaa gcagtagctg accgctgtac agttatccgc cgtggaaaag
    17761 gaatcggtac agtcaacgtt aaagacgtaa cctcacaaca attagccgat atgatggttg
    17821 gaagaacggt ttcattcaaa acgatgaaaa aagaagcgaa gcctcaagaa gtcgttttgt
    17881 ctattgaaaa tctagtggtg aaagaaaatc gtggattaga agccgtgaaa aacttgaact
    17941 tagaggttcg tgctggcgaa gtacttggta tcgctggaat cgatggaaac gggcagtcgg
    18001 agttgatcca agctttgact ggtttgcgaa aggcagaaag cggacatatc aagctaaaag
    18061 gggaagacat cactaataaa aaaccccgaa aaatcactga acatggtgta ggacacgtgc
    18121 cagaagaccg tcataaatac gggttagtcc tagatatgac attatctgaa aatatcgcac
    18181 tacaaaccta tcatcaaaaa ccttacagta aaaacggcat gctgaattat tcagtaatga
    18241 atgaacatgc cagagaattg atcgaagaat atgatgttcg aacaacgaat gaacttgttc
    18301 ccgcaaaagc tttatcaggc ggaaatcagc aaaaagcaat tatcgctcgt gaagtcgacc
    18361 gagatcctga tctgttgatt gttgcaaatc caactcgtgg actggatgta ggtgcgatcg
    18421 aatttattca taaacgtctg atcgaacaaa gggacaaata caaagcagtg ttactgatca
    18481 gtttcgaatt agaagaaatt ttaaatgttt cggatcgtat tgctgttatc catgaaggag
    18541 aaattgtcgg tatcgttgat ccgaaagaaa catctgaaaa tgaattaggt ctattgatgg
    18601 cgggctatac cttagaagaa gcacgaaaag agttagaaaa gggggctggc gcacatgaat
    18661 gatcgcagcg aaaaattccg taatttatta gtgcctgtac tttcagttgt tttaggattg
    18721 atcttaggcg cgatattgat ggctgctttt ggttttcagc ccatccaagg ttattcttct
    18781 atgctagatg cagcccttgg ttcgcaaaga gggataggtg aaacgttaag gcaagcaacg
    18841 ccgttgattt ttacagcatt aggtttttct gtagctaact cagctgggtt tttcaatatt
    18901 gggctttctg gacaagcact ttgcgggtgg atcgtcagtg tctgggtggc acttgcgttt
    18961 ccagatctgc caaaagttgt tttgcttccg ttatgcgtga tcttaggtgc cttagctggt
    19021 gcgatggcag cggctgttcc aggtttactt cgagcatttt ttggaacaag tgaagtgatc
    19081 gtaacaatca tgatgaacta tatcttatta tacgtaagca catttgcttt acatgaagtg
    19141 atgcctgaaa gctatcgctc aagtttggac tcttccaata tgatcagtca aaatgcctca
    19201 ctgcggttac catggttaac acaagctttt ggcggctctc gcgtaaactc aggattattt
    19261 ttagcaatca tcgcgcttat tttcgtttgg ctgctgatga agaaaacgac tttaggattt
    19321 gaaatccgtt ctgttggatt gaatcctttt gcttctgagt atgcaggaat gagtagtaaa
    19381 cgtacgatcg ttttgtcgat ggtcatttct ggcgctttgg cagggcttgg cggtgtggtt
    19441 gaaggattag gaacgtatca aaacttcttt gttcaaacga cttcgctatc tattggattt
    19501 gacggaatgg ctgtttcctt gcttggttct ggatcagcaa tcggtattct tttatcagcg
    19561 atcttgttca gtattttgaa gatcggtgga cttggcatgc agacaggagc aggtgttccg
    19621 tttgagatcg tcaatgtatc gattgctttg attatcttct ttgtcgggat caacttcctg
    19681 attcgtttcg tcatggctaa agtcttccaa gggaaaaaac aagaagagat cgttgcgaca
    19741 atagaatcga aaccaacgaa tcaaaaccat gaagggggcg aactataatg tcgactatcg
    19801 aattaattgc cttgatcctt acttctacac tggtttattc aacaccgcta attctaacgt
    19861 cactaggcgg aactttttct gaacgaagcg ggatcgtaaa cgtagggcta gagggaatca
    19921 tggtcatggg tgcttttagt tccatcgttt tcaacttgac gatgagtcca tcacttggtt
    19981 ctatgacacc atggatcgct attttagtgg gtggcgtcgt aggtatccta ttttctctga
    20041 ttcacgcagt agcaacagtc agtttgcgtg ctgaccatat tatcagcggg acagtaatta
    20101 atctgatggc acctgcttta ggtgttttct tgatcaaagc atggtatggt aaaggtcaaa
    20161 ctgataacat cacgcagaat ctggggtatt tttctttccc aggattagca gatattccgg
    20221 tcattggacc aattttcttt aaaaatacat tcttacctgc ttattgtgct attttgattg
    20281 ctgtcctagc ttgggtgatc atcttcaaga cgaaattcgg cttgcgcctc cgttcagttg
    20341 gtgaaaatcc tcaagctgcc gacacattag gtatcaatgt atatgctatg cgttattcag
    20401 gcgtactgat ttctggcttg ttaggcggta tgggcggtgc tgtttttgct cagtcgattt
    20461 ccggtaactt ttcagctggt acgatcgttg gacaagggtt catctccatg gctgctatga
    20521 tctttggtaa gtggaatcct ttaggggcaa tgggtgcctc attgttcttt ggttttgctc
    20581 aaagcttgag tatcattggc gcacaattac cagttatttc ttcgattccg ccagtattgc
    20641 tgcaaattgc tccatatttg ctgacaatca ttgtgctggt tgttttctta gggaaagctt
    20701 ctggtccaaa agcaaacggg aagaactata tcaaatcaaa ataatctttt agcgaataaa
    20761 aagggtgggt tacgggaagt gtttttcgtc tcgccctttt tgttttaaag gcaataaaaa
    20821 taaaaagaaa gaaggaataa tcatgtttaa acgcgtacat ctgatcgtca tggattcagt
    20881 aggaattgga gaagcaccag atgccgataa gttcggtgat gttggttctg atacattagg
    20941 acacattgcg aaagaagcag gattgaacgt tccacattta gagcagcttg gtttaggaac
    21001 gatccgtcct cttgaaggtg tggaatcagt gaaagatcat caaggctatg cgacgaaatt
    21061 agaagaagtt tctgtaggga aagatacgat gacaggccac tgggaaatca tgggattgaa
    21121 tatcaaaact ccatttcgtg tgtttcctaa tggattccct gatgatctga tcaaacaaat
    21181 cgaagaattt tctggccgta aagtcgtatg taacaaacct tatagcggaa cagctgttat
    21241 tgacgattac ggtgaacacc aaatgaaaac aggcgattta attgtatata cttctgccga
    21301 tcctgtcctt cagatcgcgg cacatgaaga aatcattcct ttagaagaat tatatcggat
    21361 ttgccaattc gttcgtgata taacaaaaga tgatccgtat atgattggcc gaatcattgc
    21421 acgtccgtac gtgggtgaac caggagactt cacacgaaca agcaatcgcc atgattatgc
    21481 gttgaatcca ttcggacaca cagtccttga ttctttgaaa gaagcaggaa aggacgtgat
    21541 tgctgttgga aagatcaacg atattttcaa cggacaagga atcactgaat ctgtccgcac
    21601 gaaaagcaat atggatggcg ttgaccagct gctgaaagtt atgaaaaacg atttttcagg
    21661 aatcagcttt accaatttag tagattttga tgcattatac ggtcatcgtc gggatgtagt
    21721 cggctatgcg catgcgattg aagaatttga cttacgaatt ccagaattgc ttgaagcgat
    21781 ggaagaagac gacttgctaa tgattacagc cgatcacgga aacgatccga cgtttactgg
    21841 taccgatcat acgcgagaat atgtaccgtt attggtttac agtaaaaaaa tgaaagaaca
    21901 aggggcattg cctcaaggat attattcaga tatttcagca acgattgctg agaactttag
    21961 tgtttcttcg actgaaaatg gcgaaagctt cttgaaatta ttaaaataat ctttttaaat
    22021 aggagaaagc aaatgaccaa gttaagcgag atgttacaag aaacgaccca gtttatcctt
    22081 gataaaggag taaaagaaat tgattttggt ttgattctag gttcaggatt aggtgaatta
    22141 gcagatgaaa tcgaagatgc tgtaatgatt tcttatgatg agatcccgtt ctttcctgtt
    22201 tcgacagtag ttgggcatgc aggaaagcta gtttatgggt ctctggcagg caagaaagtg
    22261 ttagccatgc aagggagatt ccactactat gaaggtcatt ccatgcaaac agtgacctat
    22321 ccagtacgtg tgatggccgg actaggggcc cattcagtca ttgtgactaa tgcatgtggt
    22381 ggtgtcaacg aatcatttgt accaggagat ttgatgctga ttaccgatca tatcaatttt
    22441 actggtgaaa atcctttgat cggtccgaat gaagatgcaa tgggccctag atttccagat
    22501 atgagtcaag cttatacgcc tgactatcaa gcaacagcaa aagcagcagc agaaaaacta
    22561 ggtttggatc ttaaagaagg cgtatatatg ggctattctg gaccaactta tgaaacgcct
    22621 gcggagatcc gaatgtctcg gacaatagga gccgatgcag tagggatgtc gactgttcca
    22681 gaagtcatcg tagcagctca cagcggatta aaagtgttag gaatctcttg tattaccaac
    22741 ttagcagcag gtatgcaagc gaatctgagc catgaagaag ttgtagaaac aactcagaga
    22801 gtcaaacaat cttttaaagc attggttaaa gaaacattga cattattgta ataggaggaa
    22861 caataaaatg agtgtgcata ttgaagcgaa agaaggcgag atcgccgata agattttact
    22921 tccgggagat cctttgcgag ctaaatatat tgcggagact tttttggaag atccagtttg
    22981 ttacaatcag gtacgcggta tgttaggctt taccggcaaa tataaaggag aacgtatttc
    23041 tgttcaagga acaggaatgg gaatgccatc agccaccatc tatgcccatg aattgatcca
    23101 gtcgtatggt gtaaagaaat tgattcgagt aggtacatgc ggtgcattgt caaaagacgt
    23161 ccatgttcgt gatttagtac tagcacaagg tgcagcgacc agttcttcga tgatcgaaaa
    23221 aaatttccaa gcctttcatt ttcctccaat cagtgatttc aatctattac tgaaagcata
    23281 tgagatagct aaagaaaagg gctatacggt tcatgtagga aatgttcttt cggaagattc
    23341 tttctacaaa gatgatttga cagagacgtt ccaattagct gaacttggcg ttttaggagt
    23401 ggaaatggaa gcagcggctc tttattactt aggcgcgaaa taccatgtgc agacattaag
    23461 tttgatgacc gttagcgacc atttgatcac cggtgaagaa acaacagctg cagaacgtca
    23521 atctacgttc aatgaaatga tcgaagtagg attggaaaca gcgatagcag aataagaagg
    23581 aaatgaaaaa gctgccctgc attaagtaat aagaacatcc aagcaaaaat agctcctcat
    23641 gtttttgact tggctggact tattattgca aatgtaagct tttgaacacc gtttaccaag
    23701 aggttgtgaa tctgccgttt aactctgagt cacaaggaac aatttcaaaa aacagctttt
    23761 catgtttgat gaatattgtg acttgtgacc gagaagttgg caggagaaaa ccgtttatac
    23821 gaaaaaaggg actgtgacaa atcgtgtcac agtccctttt ttatagtgtt ttttataggt
    23881 agtatttttt agcaactgtt aagtcatcat ttaattcata aacaagtggt tgacctgttg
    23941 gaatttcaag atccatgatg tcttcgtcag agataccttc gatatgttta gctagggcgc
    24001 gtaatgagtt accatgtgca gctactaaaa cagttttgtt gtctagtaat gctggagcaa
    24061 tttcatcttg ccagaatggc aaagcgcgtt ctaatgtcac tttcaagttt tctccaccag
    24121 gaatatcgcg tttgtctaac attgcgtaac gacggtcatt tgctgcagaa ccttcgtcag
    24181 tttcttccat taatggagga agagtatcgt aagaacgacg ccagatgtgc acttgttcgt
    24241 cgccgtattt ttcagctgtt tcttttttgt tcaaaccttg taatttacca tagtgacgtt
    24301 cgtttaagcg ccatgatttg atttgtggta cccaaagttg atctgaatat tctaagatca
    24361 agttacatgt tttgattgca cgagttaaaa cagaagtgta agcaacatca aattcgattc
    24421 cagcttcttt gattttgcgt ccgccttctt ttgcttcttc gattccttct ggtgctagat
    24481 ttacatcagc ccaaccagta aattggttca atgcattcca ttcactaaga ccgtgacgag
    24541 aaaaaactaa ttttggcatg tttagctctc cttttaagat aggtttatat ttctgaaatt
    24601 ttcagttctg ttttattgta caattttttg gaagagattt ccatcgaaaa attagataag
    24661 attctatgag aaaacaagta gagaatataa aaaaacgaac agtgcccacg caactgttcg
    24721 tttttaggta ttagatgcat aattgctcga tttctttttc aggtaaattg atatatttat
    24781 aacatgttcc aactgaaaca ccgcattttg aagaaataaa ttggatcgtt tctttttttt
    24841 cataatagag acgtcggatt tttttgatcg tttttgcatc gatttttggt cttccgccga
    24901 tttttccttt tttacgagct tcatctaatc caactaatgt acgttctttg atcaatgcgc
    24961 actccatgtt tgctaggttt tccattaatt ggaaatagat ttttcccatt tcgcgcgtat
    25021 cgattccttc atcaagacta atcaagtgca gcccgcgatc tttgaataat tgcgtgagtt
    25081 ctgttaattg ccgcgtagat ttgcctaatc gatgaagttg gcaaatgact agtgcatcgc
    25141 cttgttgcat gtcagcgatc actgactcca aatgaacagc agtagaatca ttttgttcca
    25201 cttcatatgt ctcatggaaa atttcttgac aaccaaagtc agataattga tcaacctgag
    25261 ttgttaaatc aacgtcattt atggttgtgc gtgcataacc aataattttc atggtgattc
    25321 ctttctagca gtgattttct aactggatat aacagtaaaa atgtagcata aagtacgtca
    25381 aaaagatagt tttttcgcaa attttccgct gtcttttttt attggatgat caagcagttt
    25441 ttctttattt acgacggatt gcgtcaagaa tctcaacgcc tttttctgag ccgacaacta
    25501 ctttagaaac catgtttagg aaaagcccat gttctactac cccaacaagt tcatctaaat
    25561 aggtagctaa gctttctggc tgttcgatga cttctagatg cagatcaatg atgtaatgac
    25621 caccatctgt ggttaagact tctccgtttt cgtctgtacg caagacgggt tggaatcttt
    25681 tttcttcaaa aatatgaagt agctgttggg agccataagg gatcacttca actgggagtg
    25741 ggaacttccc aagtttatcg actaatttag aagaatcaac gatccaaatc gtttcttttg
    25801 agtacgtagc gactattttc tcgaaaagca aagcagcgcc gccgcctttg atcccttgaa
    25861 aatcagcact gatttcatca gcaccgtcta ttgtcaaatc gactacgggg acttcatcaa
    25921 tactctttaa cggtattcct aatttttccg cttgttcttt tgtcgcattt gaagtagtaa
    25981 ctcctgtgat acgtaaacct tcttcacgca tacggcgacc aatctcttct accatataat
    26041 aggcggttga accggtcccg agacctacga tcatcccgtc ctttacgaat ttcgcggctt
    26101 caatgccagc taattgtttt aaattcatct ttgcgtccac ctttttttag tttgagcatc
    26161 gtgctttcct cactatttta gcgtgattta cggtaaaaag ataccattct tttttattcc
    26221 aataaagctt tttcaaaaaa aattgcatct cttgttgaca tgttttttca ttcgtgatat
    26281 tctaacaaag gtgctttatc tacaggtttc tgtgaagaaa cgtgctgact gtttatcaaa
    26341 gcatatttga taaggatgca gacattgcat ttatttttta tcacaaaaaa agaaccacct
    26401 ggatgtgtgg aactagacgc aaatcgtggg aaggaggaaa caaggaatgc ctacaattaa
    26461 ccaattagta cgtaaacctc gtaaatcaaa ggtggaaaaa tctaattcac ctgctttgaa
    26521 caaaggatat aacagcttta agaaaactca aactaacgta aactcacctc aaaaacgtgg
    26581 ggtagcaact cgtgtgggta caatgacacc taaaaaaccg aactcagctt tacgtaaata
    26641 tgcacgtgtt cgtttgtcaa acttaattga agttactgct tatatcccag ggattggtca
    26701 taacttacaa gaacacagtg ttgtattatt acgcggtggt cgtgtaaaag acttaccagg
    26761 ggtacgttac catatcgttc gcggtgcttt agatactgct ggtgttaacg accgtaaaca
    26821 aagccgttct aaatacggaa ctaaaaaacc taaagcttaa ttttaattat taaacaaatg
    26881 aaacttgctt ctactatata gaagtaatta ctatgaacaa tatttcggaa ggaggagtta
    26941 cggatgccac gtaaaggtcc tgttgcaaaa cgtgatgttt taccagatcc tatttataac
    27001 tcaaaattag taactcgctt gatcaaccgt gtaatggttg acggaaaacg cgggattgca
    27061 gctaacatta tctataatgc ctttgacttg atcaaagaat ctacaggtaa cgatccatta
    27121 gaagtgtttg aacaagcaat gaaaaacgtt caacctgttc ttgaagttaa agcacgtcgt
    27181 gttgggggtt caaactacca agtaccagtt gaagttcgcc ctgaacgccg cgttactcta
    27241 ggtcttcgtt gggtagtaaa ctacgctcgc ttgcgcggtg aacacacaat ggaagaacgc
    27301 ttagcaaaag aaatcatgga tgctgcaaac aacacaggtg cttctgttaa aaaacgtgaa
    27361 gacactcata aaatggctga tgccaaccgt gcgtttgctc actatcgctg gtaagattct
    27421 ctctgtttgg tcggctttta taagtcgacc tcagatagtg tccttatttt tagcaactag
    27481 atagtaaaga gaggagaaac aaggagaatg accagagaat tttctttaca aaacactcgt
    27541 aatatcggta tcatggccca catcgatgct ggtaaaacga ctgctactga acgtatcttg
    27601 tactatactg gtaaaatcca taaaattggt gaaactcatg aaggagcttc acaaatggac
    27661 tggatggaac aagaacaaga acgtggtatt acgatcactt ctgctgctac tacagcacaa
    27721 tggaagggtc accgaatcaa catcatcgac acaccaggac acgtagactt cacagttgaa
    27781 gttgaacgtt cattgcgtgt acttgatggt gctgtaactg ttcttgatgc acaatcaggt
    27841 gttgaacctc aaactgaaac agtttggcgt caagcaacaa cttatggtgt acctcgtatc
    27901 gtatttgcaa acaaaatgga caagatcggt gctgacttct tatattcagt atcaacttta
    27961 catgaccgtt tgcaagcaaa cgctcaccca atccaattac caattggtgc ggaagacgac
    28021 ttcacaggaa ttatcgacct tgtaacaatg aaggctgaaa tgtatacaaa cgatcttgga
    28081 acagagatcg aagaaacaga aattcctgaa gaataccgcg aattagctga agaatggcgc
    28141 gaaaaattgg ttgaagctgt agctgaaact gatgaagaac ttactttgaa atacttagaa
    28201 ggtgaagaaa tcactgaagc tgagttaaaa gaaggtatcc gccgcgcaac agttaacgta
    28261 gaattctacc cagtactatg tggttcagca tttaagaaca aaggggttca attactattg
    28321 gacgctgttc ttgattactt gccatcaccg ttagatatcc cagctatcaa agggattgac
    28381 cctaaaactg atgaagaagt agaacgtcct gcagacgaca gcgctccttt ctcagcttta
    28441 gcatttaaag ttatgactga cccattcgta ggtcgtttaa ctttcttccg tgtgtactct
    28501 ggtgttttac aatcaggttc atacgtacaa aacgcaacaa aaggcaaacg tgaacgtgtt
    28561 ggtcgtatcc tacagatgca tgccaactct cgttcagaaa tctctgaagt ttacgctgga
    28621 gatatcgctg ctgctgttgg tttgaaagac acaactactg gggacacact atgtgatgaa
    28681 aagagtctag ttattcttga atccatggaa ttcccagaac cagttatcca agtcgctatt
    28741 gaacctaaat caaaagctga ccaagataaa atgggtgtgg ctttacaaaa actatctgaa
    28801 gaagatccta ctttccgtgc tgaaacaaac gtggaaacag gcgaaacaat catcgctggt
    28861 atgggtgaac ttcacttaga tatcatcgtt gaccgtatgc gtcgtgaatt caaagttgaa
    28921 gctaacgtag gtgcgcctca agtatcttac cgtgaaactt tccgtgctgg cactcaagcc
    28981 gaaggtaagt ttgtacgtca gtctggtggt aaaggtcaat acggtcacgt atggattgaa
    29041 tttacaccaa atgaagaagg tgctggtttc gaatttgaaa atgcgatcgt cggtggggtt
    29101 gttcctcgtg aatacattcc agctgttgaa actggtttga aagatgctat ggaaaatggt
    29161 gtcttagctg gctatccatt agtagatatc aaagctaaac tttacgatgg ttcataccat
    29221 gatgtcgatt caaatgaaac tgccttccgt gttgctgctt ctatggcatt acgtgcagca
    29281 gctaagaaag ctaatccagt aatccttgaa ccaatcatgg ctgtagaagt tgttatccct
    29341 gaagattatt taggtgatgt tatgggtcac gtaactgctc gtcgtggacg tgttgaaggt
    29401 atggaagctc gcgctggcgg ccaacaagtt gttcgtgcga tggttccact tgctgaaatg
    29461 tttggttatg ctacaactct tcgttctgct actcaaggtc gtggtacatt tacaatgaca
    29521 tttgaccact acgaagatgt accaaaatca gtacaagaag aaatcattaa gaaaaacggc
    29581 ggaaaagctg aataatttct taaaaccgga tagtcctgca aagagattat ccaaagagac
    29641 tatattttta atatagtttc gtgtaaaata attatcgatg atatgggcat gtggtgtacc
    29701 ttgtgtacat catataccta tcagacaaat aaaaaatata ttgtattttt aggaggacat
    29761 tttaaaatgg caaaagaaaa atttgaccgt tctaaaccac acgttaacat tggtactatc
    29821 ggacacgttg accatggtaa aactacatta acagctgcaa tcacaactgt actatctaag
    29881 aaaaacggcg gccaagctat ggcttacgat caaatcgatg gtgctccaga agaacgcgaa
    29941 cgcggaatca caatctcaac agctcacgtt gagtatgaaa cagatacacg ccactacgct
    30001 cacgttgact gcccaggaca cgcggattac gttaaaaaca tgatcactgg tgctgctcaa
    30061 atggacggag ctatcttggt agtttctgct gctgacggcc caatgcctca aactcgtgaa
    30121 cacatcctat tgtctcgtca agttggtgtt ccttacatcg ttgtattctt gaacaaagta
    30181 gacatggttg atgacgaaga attactagaa ttagttgaaa tggaagttcg tgacctatta
    30241 acagaatacg aattccctgg tgacgatgtt cctgtagttg ctggatcagc tttgaaagct
    30301 ctagaaggcg acgcttcata cgaagaaaaa attcttgaat taatggctgc agttgacgaa
    30361 tacatcccaa ctccagaacg tgacaacgac aaaccattca tgatgccagt tgaagacgtg
    30421 ttctcaatta ctggacgtgg tactgttgct acaggtcgtg ttgaacgtgg acaagttcgc
    30481 gttggtgacg aagttgaagt tgttggtatt gctgaagaaa cttcaaaaac aacagttact
    30541 ggtgttgaaa tgttccgtaa attgttagac tacgctgaag ctggagacaa cattggtgct
    30601 ttactacgtg gtgttgcacg tgaagacatc caacgtggac aagttttagc taaaccaggt
    30661 acaatcacac ctcatacaaa attctctgca gaagtatacg tgttgacaaa agaagaaggt
    30721 ggacgtcata ctccattctt cactaactac cgtccacaat tctacttccg tacaactgac
    30781 gtaacaggtg ttgttgaatt accagaagga actgaaatgg ttatgcctgg cgacaacgta
    30841 acaatggaag ttgaattaat ccacccaatc gctatcgaaa acggtactaa attctcaatc
    30901 cgtgaaggcg gacgtacagt tggtgccggt gtcgttacag aaatcaaagc ttaattcact
    30961 taagttttaa agtaaagcaa catttattcg gacgtaatcg ctcggaacga aggtcttagg
    31021 atctttgttc cgagcttttt tatttattgc aggatcagga aaaaagacag gagtattata
    31081 aatagaagaa acacgaaata aaagagtcag tattttctaa atgaaacttt atgaaaacaa
    31141 aatgtttttt acaagaaaaa gccttgcttt gtgagtttat atatattata atgacaaagg
    31201 tgcgtttact ttagtgaatt gcacagacgt aatcagaatg atttcgtcgg ctaagaaacg
    31261 agaggttgcg acacgcccgg acgctttgcc acgaacgagc gtgacggaaa ttttcgtgga
    31321 gctatgtcta cttttcaaaa taggcgaagg agggaacaag atggcaaaac aaaaaattcg
    31381 tatccgttta aaagcgtatg aacaccgtat tttagatcaa tcagcggata aaatcgtgga
    31441 aactgcaaaa agaactggag ctagcgtgtc tggtccaatt ccattaccaa ctgaacgcag
    31501 tctttacaca atcatccgtg cgactcataa atacaaagat tcacgcgaac aattcgaaat
    31561 gcgtacacac aaacgtctta tcgacattgt gaacccaaca ccaaaaactg ttgatgcttt
    31621 gatgaagcta gacttaccat ctggtgtaaa catcgaaatc aaattataaa actaaaacaa
    31681 aaataatgga ggtgtactca tgaccaaagg aatcttaggg aaaaaagtgg gaatgacaca
    31741 aatcttcact gaatctggtg aattaattcc agtaactgtt gtagaagcta ctccaaacgt
    31801 agtactacaa gtgaaaacaa tggaaacgga tggctacgaa gctattcaag ttggttacca
    31861 agatatgcgt gaagttttat caaacaaacc tgcgaaaggt catgttgcaa aagcaaacac
    31921 ggctcctaag cgcttcattc gtgaattcaa aaatgttgag ctaggagaat atgaagtagg
    31981 aaaagaaatt acagtagatg ttttccaagc aggagacatt attgatgtaa caggtactac
    32041 gaaaggtaaa ggattccaag gggttatcaa acgccacgga caaagccgcg gaccaatggc
    32101 tcacggttct cgttaccatc gtcgtcctgg gtcaatgggt ccagttgcac ctaaccgtgt
    32161 atttaaaaac aaacgtcttg ctggccgtat gggtggcaac cgcgtaacta tccaaaactt
    32221 ggaagttgta cgtgtcgatg cagaaagaaa tgttatcttg atcaaaggta atgttcctgg
    32281 agctaaaaaa tctttaatta ctatcaaatc agctgtgaaa gctaaataat tgtaggaaga
    32341 aaggaggaac taaggaatgc cgaatgtagc attattcaaa caagatggaa gccaaaacgg
    32401 tgaaatcaca cttaacgaag aaatcttcgg aattgaacct aatgaaagtg ttgtttacga
    32461 tgcaatcgta atgcaacgtg cgtcattaag acaaggaaca cacgcggtta aaaaccgtag
    32521 cgctgttcgc ggcggcggac gtaaaccatg gcgtcaaaaa ggaacaggtc gtgcacgtca
    32581 aggatctatc cgctcaccac aatggcgtgg aggtggcgta gtctttggac caacaccacg
    32641 ttcttacagc tacaaacttc ctaaaaaagt tcgtcgttta gcaatgaaat ctgtattgtc
    32701 agaaaaagtt gctgaaaaca acttggtagc aatcgaaggc ttgaacttcg acgcaccaaa
    32761 aacaaaagaa ttcaaacaag ttcttgctaa cttatctatc gactctaaag tattggttgt
    32821 tctagaaaaa ggtaacgact ttgctgcttt atcagcacgt aacttaccaa acgtttctgt
    32881 agtaacttct gataatgtga gtgtgctaga cgttgtatca aatacaaaag tgttagcaac
    32941 acaaactgct cttactcaaa ttgaggaggt gcttgcataa tgaacttact agacgtaatc
    33001 aaacgcccag tgatcactga aaaatcaatg cttgccatgg atgacaagaa atacactttt
    33061 gaagtggaca ctcgcgcaaa caaaacttta gtaaaacaag cggtagaagc tgcttttgac
    33121 gttaaagtca aaaacgtgaa catcgtgaac gtacgtgcga aattcaaacg tatgggcaaa
    33181 tatgcaggat atacaaaaaa acgtcgcaaa gcgatcgtta cattgactga agattcaaaa
    33241 gaaatccaat tattcgaagc tgctgaataa gcactaatag ataactcatt aaattaggag
    33301 ggaaaaacgt ggcgattaaa aagtacaaac ctacctcaaa tggtcgtcgt aacatgacag
    33361 gttctgattt tgctgaaatc acaacatcaa cacctgaaaa atcattgtta caaccattga
    33421 aaaaccatgc tggacgtaac aacaacggcc gcatcactgt acgtcatcaa ggtggcggac
    33481 acaaacgcca atatcgcgtg atcgacttca aacgtaacaa agataacgtt gttgcaactg
    33541 ttcaaacgat cgaatatgat ccaaaccgtt cagctaacat cgcgttagta cactatgaag
    33601 atggagtgaa agcttacatc ttagcaccta aaggactaga agtgggtatg aaacttgttt
    33661 caggtccaga agcagatatc aaagtcggaa atgctcttcc attagaaaac attccagttg
    33721 gtactgttat ccacaacatt gaaatgaaac ctggtaaagg tggacaattg atccgttctg
    33781 ctggtacaag tgctcaagta cttggtaaag aaggcaaata cgtattgatc cgcttgaact
    33841 ctggtgaagt tcgtatgatc ttagctgcat gccgtgctac aatcggttct gtaggtaacg
    33901 aacaacacga attaatcaac atcggtaaag ctggacgctc tcgttggatg cgtaaacgcc
    33961 caactgtacg tggtagcgta atgaacccta atgatcaccc acacggtggt ggtgaaggta
    34021 aagctcctat cggacgtaaa gcaccagttt ctccttgggg tcaaccagct cttggttaca
    34081 aaactcgtaa caaaaaagct aaatcagaca aacttattgt tcgtcgtcgc aaaaataaat
    34141 aattactttg accatgtgtc gcacattttg agaggaggtt caccatgggt cgtagtttaa
    34201 agaaaggacc ttttgtcgat gagcatttaa tgaaaaaagt tgaagctcaa gcaggggttg
    34261 aaaagaaaaa agttatcaaa acttggtcac gccgttcaac agttttccca agttttgttg
    34321 gatataccat cgcagtttat gatggacgta aacatgtacc agtttacatc caagaagaca
    34381 tggtaggaca caaattaggt gaatttgcac ctacaagaac ttatcgtgga catgctgctg
    34441 acgataagaa aacgaaacgc taatttgagg ggaggataaa tccaaatggc agaacaaatc
    34501 acatcagcta aagcaactgc aaaaacagtt cgcacttcac ctcgaaaagc ccgtttagta
    34561 attgacctta tcagaggtaa aagtgtcgca gatgcaattt caatcttgaa attcacacca
    34621 aacaaatctg ctggaatcat tgaaaaagta ttgatgtcag cagttgcgaa tgcagaaaat
    34681 aactttgact tagatgttga aaacttggta gtaagcgaag cttttgttaa cgaaggacct
    34741 acaatgaaac gtttccgtcc tcgtgcaaaa ggatcagctt ctccaatcaa caaacgtaca
    34801 agtcatatca cggtagtcgt atcagaaaaa taaggaggga taatcagtgg gtcaaaaagt
    34861 acatccaatt ggaatgcgtg taggcatcat ccgcgactgg gatgctaaat ggtatgctga
    34921 aaaagagtat gctgagttct tacacgaaga tttaagaatc cgtaaattta tttcaactaa
    34981 acttgctgat gctgctgtat caacagttga aatcgaacgc gcaacaggtc gcgtaaacat
    35041 ttcaattcac acagctaaac caggtatggt tattggtaaa ggcggttctg aagtcgaaag
    35101 cctaagaaaa gaattaaaca aattaactgg taaaagaatc catatcaata tcgtggaaat
    35161 caaaaaacca gatctagatg caaaattagt aggtgaagga atcgcacgtc aattagaaaa
    35221 ccgtgttgct ttccgtcgcg ctcaaaaaca agccatccaa cgctcaatgc gtgctggcgc
    35281 tcaagggatc aaaactcaag tatctggtcg tctaaacgga gcagatatcg ctcgttcaga
    35341 aggatactca gaaggaactg ttcctcttca tactttacgt gcggacattg attacgcatg
    35401 ggaagaagca gatacaacat acggaaaact aggagttaaa gtgtggatct atcgtggaga
    35461 aattcttcca actaaaaaaa acactgagaa aggagggaaa taatcatgtt agtacctaaa
    35521 cgtgtaaaac accgtcgtga attccgcgga aaaatgcgcg gggaagctaa aggcggaaaa
    35581 gaagtagcat tcggtgaata cggtttgcaa gctgttgatt cacattggat cacaaaccgc
    35641 caaatcgaag ctgctcgtat cgcaatgact cgttacatga aacgtggtgg gaaagtatgg
    35701 attaaaattt tccctcacaa atcttatact gccaaagcaa ttggggtacg tatgggttct
    35761 ggtaaagggg cacctgaagg atgggttgca ccagtaaaac gtggtaaaat catgtttgaa
    35821 atcgcaggcg ttcctgaaga agtagctcgt gaagcgttac gtctagcttc tcacaaatta
    35881 ccaatgaaaa ctaagatcgt aaaacgtgag gaaatgggtg gtgaatcgaa tgaaggttaa
    35941 agaaatcaga gaattaacca ctgccgaaat gcttgatcaa gaaaaacaat taaaagaaga
    36001 attgtttaat cttagattcc aattagcaac aggtcaatta gaaaacactg cacgtattaa
    36061 agaagtacgt aaatcgattg cacgcatcaa aactgtgttg cgtgaacaag ctaagtaatt
    36121 atggaaggag gccattaagc gtatgactga aggaagaaat caacgcaaag tttatcaagg
    36181 tcgcgtggtt tcagacaaaa tggataaaac aattaccgtt gctgtcgaaa caaagaaaaa
    36241 ccaccctatc tatggcaaac gcatgaacta ttctaagaaa tacaaagtgc atgatgaaaa
    36301 caacacagca aaagttggcg acatcgtgag aattatggaa actcgtccat tatcagctac
    36361 aaaacgtttc cgtttgttgg aagtagtcga agaagcagtt attatctaat aacacgagat
    36421 tttcctatat agattgaatg caaaatctga aaggaggata cacaacgtga tccaagcaga
    36481 aagtcgttta aaagtcgctg ataactcagg cgcacgtgaa attttgacga tcaaagtcct
    36541 tggtggatct ggtcgtaaaa ctgctaacat cggtgatgtg atcgttgcta cggttaaaca
    36601 agcaacgcca ggtggggttg tcaaaaaagg tgacgtcgtt aaagccgtta tcgttcgaac
    36661 aaaatcagga gctcgtcgta cagacggttc atatattaaa tttgatgaaa atgctgcagt
    36721 gattattcgt gatgataaaa gccctcgtgg aacacgtatc tttgggccag ttgcacgtga
    36781 actacgtgaa aacaacttca tgaagatcgt ttcactagca ccagaagttt tataatcttg
    36841 agacacttat caaaggaggt gcgaaacagt tatgtttgtt aaaaaaggcg ataaagttaa
    36901 agttatcact ggaaaagaca aaaacaaaga aggcgttgta ctagcagcgt tcccgaaaaa
    36961 agataaagtt attgtcgaag gtgtaaatgt cgttaaaaaa caccaaaaac cttctcaagc
    37021 tgctcctcaa ggtggaattg ttgagatgga agcgccaatt catgtttcta acgtgatggt
    37081 ggttgactct actggtgtag ctggccgagt tggctacaaa gaagtagacg gcaaaaaagt
    37141 ccgtgtttct aaaaaaaccg gtgaagtttt agataaataa aagtttaagg aaggagggtc
    37201 ttactgaatg aaccgcctaa aagaaaaata tcttaaagaa gtaactccat cattgatgga
    37261 aaaatttaac tatagctctg ttatgcaaac acctaaagtt gaaaaaatcg tcatcaacat
    37321 gggtgttggt gatgcggtat caaacgctaa aaacttagat aaagcagttg aagaattaac
    37381 tttgatcact ggtcaaaaac caatgattac aaaagctaaa aaatcaatcg ctggattccg
    37441 tttacgtgaa ggaatgccaa ttggtgcaaa agttacattg cgcggagaaa gaatgtacga
    37501 attcttagac aaattggttt ctgtttcact accacgtgta cgtgacttcc atggtgtaag
    37561 taaaaaagcc tttgacggtc gtggaaacta tactttaggt attaaagaac aattgatctt
    37621 cccagaagta gattatgatt tagtagataa agtacgtggt atggacatcg ttattgtaac
    37681 aacagcaaac tctgatgaag aatctcgtga gttgttgaca caattaggca tgccattcca
    37741 aaaataatta aaggaggcga actacgtggc taaaaaatca atgattgcta aaaacaaacg
    37801 tcctgcaaaa ttttcaacac aagcttatac tcgttgcgaa cgttgcggac gtccacattc
    37861 agtttatcgt aagttccatc tttgccgtat ttgcttccgc gaacttgcct ataaaggtca
    37921 aattcccggc gtgaagaaag ctagctggta aaaagtaaat tcgcataaga aggaggtaaa
    37981 agatcaatgg tcatgacaga tccaattgca gattttctaa ctcgtatccg taacgcaaac
    38041 atggttaaac atgagtcttt agaagtacct gcatcaaaaa ttaaacgtga catcgctgaa
    38101 atcttgaaac gtgaaggttt cgttcgtgat gttgaataca tcgaagatga caaacaaggc
    38161 gtgatccgtg ttttcctaaa atttggtaaa aacggcgaac gtgttatcac aaacttaaaa
    38221 cgtatctcta aaccaggttt gcgtgcttat gtcaaagctg acgaagtacc aaaagtattg
    38281 aacggtttag gtatcgcaat catttcgact tctgaaggtg ttgtcactga taaagaagct
    38341 cgtgaaaaaa atatcggcgg cgaagttatc gcttacgtat ggtaatcaag tagaatctac
    38401 aaggaggtgt ctttaagtga gccgtatcgg aaataaagtc gttgttatcc ctgaaggagt
    38461 aacagttact caagacggaa acaacgttac agttaaagga ccaaaaggtg aattgactcg
    38521 tactttttca gcagatatca aaatgaatat cgaaggaaac gaagtaactt tcactcgtcc
    38581 aaacgacagt aaagaaatga aaacaattca tggaacaact cgtgcgaact tcaacaacat
    38641 ggttgtaggt gtcagcgaag gtttccaaaa agcgttagaa ctaatcgggg ttgggtaccg
    38701 tgcacaatta caaggatcaa aacttgtatt gaacgttggt tactcacatc cagtagaaat
    38761 cacaccacca gctggagtaa ctgttgaagt accagctaac acacaagtaa ttgttaaagg
    38821 tgcgaacaaa gaagaagttg gcgaattagc tgcaaacatt cgtggaactc gtcctccaga
    38881 accttataaa ggtaaaggta ttcgttatgt tggcgaatat gtacgccgta aagaaggtaa
    38941 aactggtaaa taatcgttta gaaaaattgc atagaagcca gcagcaggaa aaaggcattt
    39001 ggctgaagtt taccttcaaa aaaatacatt tttacttgtg ccgcagcttc tatttttcga
    39061 aacgtattta ctgcttgaaa tagtagtaca ataaaaataa agaggtgaca attgtgatta
    39121 caaaaccaga taagaacaaa acacgtcaaa agagacatcg tcgtgtacgt aacaatatct
    39181 ctggtactgc tgagcgccca cgcttgaacg tttttcgttc taacaaaaac atctacgctc
    39241 aaattattga tgacgtagcg ggtgtaacgc tagcaagtgc ctctaccttg gataaagaaa
    39301 tttcaggtgg aacaaaaaca gaagcagcac aagctgtcgg taaactagtt gctgaacgtg
    39361 cagctgaaaa aggcattaaa gtagtagtct ttgaccgtgg tggatacctt taccatggcc
    39421 gtgtgcaagc tttagctgaa gctgctcgcg aaaatggact agaattttag aagaaggagg
    39481 aataccattc atggtttata tcgatccaaa acatttggaa ttagaagacc gcgttgttgc
    39541 aattaaccgt gtaacaaaag ttgttaaagg tggacgtcgt ctacgttttg ctgctttagt
    39601 tgttgtcggt gacaaaaacg gacacgtagg gtttggtaca ggtaaagctc aagaagttcc
    39661 tgaagcaatc cgtaaagcaa ttgaagatgc gaagaaaaac ttagttgaag tacctatggt
    39721 tggttcaaca atcccacacg aagtaatcgg tgcttttagt ggtggtagaa tcctaatgaa
    39781 acctgctgta gaaggttctg gggttgctgc tggtggacca gttcgtgccg tattagaatt
    39841 agctggggta gcagatatca cttctaaatc tttaggctca aatacaccaa tcaacgttgt
    39901 tcgcgcaaca gttgaaggtt tgaaacaatt gaaacgtgca gaagaagttg cagaacttcg
    39961 cggcaaatca gttgaagaat taatcggata aggaggacaa caataatggc tgaattaaaa
    40021 gttactttaa aacgcagtat tatcggacgt cctcaaaacc aacgcgatac agttaaagcg
    40081 ttaggtctta caaagattaa cagttcagtg gttaagcctt ctaatgaagc aattaaaggc
    40141 atgatcaata ctgtttctca cctagtggac gttgaagaaa tttaatttag aaaaatcata
    40201 actagaacat taaggaggtg ccaaacctat gaaacttcat gaattaaaac ctgctgaagg
    40261 ttcacgccaa gtacgtaacc gtgttggtcg tggtacttca tctggtaacg gaaaaactgc
    40321 cggacgcgga caaaaaggac aaaaagctcg ttcaggcggc ggtgtgcgtt taggatttga
    40381 agggggacaa acaccattgt tccgtcgttt accaaaacga ggattcacaa acgtgaaccg
    40441 taaagactat gcagtcatta atcttgatgt attgaaccgc tttgaagacg gagctgaagt
    40501 aactcctgtt gctttagttg aagctggaat cgtgaaaaac gaaaaagctg gaatcaaagt
    40561 attagctaat ggggaactaa acaagaaatt aactgtgaaa gcagcaaaat tctctaaagc
    40621 agctcaagaa gcaattgaag cggctggtgg atcaatcgag gtgatctaat gtttagacta
    40681 ttaaaagatg cttttaaagt aaaagacatt agatcgaaaa tcctatttac tgtatttgtg
    40741 ttgtttgtat ttcgcctagg tgcacatatt actgtaccag gcgtaaatgc tgcttcattg
    40801 caaaaccttt cttctttgcc atttttcaac atgttgaact tggtaagtgg tagtgcgatg
    40861 caaaactttt ccattttctc aatgggggtt tcaccataca ttactgcttc tatcgtcatt
    40921 cagcttcttc aaatggacat cgtgcctcga tttgttgaat ggtcgaaaca aggggaagtt
    40981 ggtcgtaaaa aactgaatca agcgactcga tatctgacat tagtattggc atttatccaa
    41041 tctgtcggta tcactgctgg attcaatcaa tacgctcaat taggtttcgt gaataatcct
    41101 aacgtagcaa cttacgtaac gatcggtttg attctaacag ccggtacgat gtttgttact
    41161 tggctaggag aacagatcac agaaaaaggt atcggaaatg gcgtatcaat gatcattttt
    41221 gcaggtatca tttctagatt gcctgaaagt atcagagaaa tttatgaaga ttactttata
    41281 aatattgatc cttcagacat atggaaatct gctatttttg tggccatttt actagtagcg
    41341 gtcttgttga tcgtgacatt cgtaacattt ttccaacaag ctgaacgcaa gatcccgatc
    41401 caatatacga aacgcgtagc tggcgcgcct acaagcagct atctgccgtt gaaagtaaat
    41461 gcagctggag ttattccagt tatctttgct agttcgttta ttgctacgcc aaatgctgtt
    41521 ttacaagcat tgagcggtag attcggttct gaaaactggt acaaagtgat gatggagatc
    41581 tttaactata atacagcacc tggtgctacg atttatacga ttctgatcgt tgccttcact
    41641 ttcttctacg cctttgttca agtaaaccct gagaaattag cggaaaactt gcaaaagcaa
    41701 ggaagttata taccaagcgt acgccctggt aaggggactg aagagtatgt ctcaagatta
    41761 ctgatgcgtt taagtacagt tggtgcagtc ttccttggtt tagtggcatt gcttccaatc
    41821 attgctcaaa tggtttggga tcttccacaa tcaatcggat taggcggtac aagcttactg
    41881 atcgttatcg gtgtagcttt agaaactgcg aaacagttag aaggcttaat gctgaaacgt
    41941 aaatatgttg gattcattga gtaagaaagc gtgttgaggt ttcccctcaa cacacgttct
    42001 cacacattta ttaggaggga aaacaatgaa cctcatttta atggggctgc caggcgcagg
    42061 taaaggaaca caagctgaaa aaatcattga tgcttatggt attccgcaca tctcaacagg
    42121 agatatgttc cgcgcagcga tgcaaaacga aacagctctt ggcctggaag caaagtctta
    42181 tatggataaa ggtgcattag ttcctgatga agtaacaaat ggaatcgtga aagagcgact
    42241 agctgaacca gatacagaga aaggcttctt attagatggc tttccacgta cgctagatca
    42301 agcaaaagct ctggacgcaa tgctaaaaga tttgaataaa aaaattgatg ctgtcatcga
    42361 tatccatgtt ggtgaagaaa tcttggtcga acgtctagcc ggccgattta tctgcagcaa
    42421 ttgtggagca acttaccata aaattttcaa ccctacaaaa gttgaagaca catgtgatcg
    42481 ttgcggtggg catgaattct atcaaagaga agatgataaa cctgagacgg ttaaaaatcg
    42541 tctggctatc aacatcaaaa atagtgaacc aatcttggct tattataaag aacaaggttt
    42601 gctaaacact attgatggtg ctcgtgaaat cgatgctgta tttgcagatg tacaaaaaat
    42661 cattgaaaaa tagcacgatt gcccgtagcc gacttgcatt atgcagttac tcttgtcaag
    42721 ttcaataaat tatgatagaa tatgtcagtc cgacttctag agaatcctct agatgatcca
    42781 ttttgagaat tttgaggtgg gaactgcagt ttccgccatt ttatcgtgtg aaaatgcgaa
    42841 acaagtacaa ggaggtactg tgcgtggcaa aagaagatat gattgaagtc gaaggtacag
    42901 tcgtcgaaac tttgccgaat gcaatgttta aagttgaact agaaaacgga caccaagttc
    42961 ttgctactgt ttcaggtaaa attcgtatgc attacatccg tatcttacct ggtgacaaag
    43021 taacggttga attgtctcca tacgatctta accgtggccg tattacttat cgctttaaat
    43081 aattgtactc cgtaaaacta caggaggtat tatcatgaaa gtaagaccat cagtaaaacc
    43141 aatgtgcgaa cattgtaaag taattcgtcg taaaggacgc gttatggtga tttgcccagc
    43201 aaatccaaaa cataaacaac gtcaaggata attggaggtg ctataaatat ggctcgtatt
    43261 gcaggagtag atattcctcg tgataaacgt gtagtagttt cacttactta tatctatggt
    43321 attggtaaca ctactgctaa aaagatctta gctgatgctg gtgtatctga agatgttcgc
    43381 gttcgtgaat taacgaatga acaaacagat gctatccgtg cagaagtaga taaattaaaa
    43441 gttgaagggg atcttcgtcg tgaagtgaac ttgaacatca aacgtttgat ggaaatcggt
    43501 tcttaccgtg gaatccgtca tcgccgtgga ttgcctgttc gtggacaaaa cacgaaaaac
    43561 aatgcacgta ctcgtaaagg cccagctcgc gctatcgctg gcaagaaaaa ataattctta
    43621 agtgaaggag gttaaaactt catggcagca aaaaaagtga atcgtaaacg ccgtgtgaag
    43681 aagaatatag aagcaggtat tgcacatatc cattctacat tcaataacac tatcatcatg
    43741 atcactgata ctcacggaaa tgcattagcg tggtcatcag ctggttcatt aggtttcaaa
    43801 ggaagcaaaa aatcaactcc ttttgccgct caaatggcag cagaaactgc tacaaaagca
    43861 gcaatggaac acggattaaa gactgttgaa gtaacagtta aaggacctgg ttctggacgt
    43921 gaagcagcaa ttcgttcatt acaagctaca ggtttagaag tgactgcaat tcgtgacgtg
    43981 actccagttc ctcataatgg tgcccgccct ccaaaacgcc gtcgtgttta atgagtgccg
    44041 ctcattttga gttttatcag taacgtcatt gaacaagaca cttttcgttt tgaaaggggt
    44101 aaagataaga atgattgagt tcgaaaaacc aagaatcgca aaaattgatg aagaaaaaga
    44161 ttatggcaag ttcatcgttg aacctcttga aagagggtat gggactactt tagggaattc
    44221 cctacgtcgt attttattat cttctttgcc aggtgctgcc atcactaata ttcaaatcga
    44281 tggtgtactg catgaattct ctaccatcaa aggtgttaga gaagacgtca ctcaaatcat
    44341 cttgaacatt aaaggcttag cgctgaaaat gtatggccaa gaagaaaaaa cccttgaaat
    44401 cgatattacc ggacctgcta cagtaactgc tggcgatatt atcgttgata gtgatgtaga
    44461 aattcttaac aaagatatgt acatctgtag tgtctctgaa ggagctacgt tccatgctcg
    44521 cttaacagtg aaacctggac gtggttatgt tcaagcagat gaaaataaaa aagaagatat
    44581 gccaattggc gttcttccag tcgattcaat ctacacacct gttcgtcgtg tgaactatca
    44641 agttgagaac acacgtgttg gacaccgtga agattttgat aaattgacga tggagatttg
    44701 gacagatggt tcaattgaac cgttggaagc aatgagtttg tctgcgaaaa tcatgactga
    44761 acatttggat atttttgtga acttaactga tgaagcaaaa aatgctgaaa tcatggtcga
    44821 aaaagaagaa acacaaaaag aaaaaatgtt ggaaatgacg atcgaagagt tagacttgtc
    44881 tgttcgctct tacaattgtt tgaaacgtgc aggaatcaat actgtacaag aacttacaaa
    44941 taaatctgag cctgaaatga tcaaagtccg taacttagga cgtaaatcat tagaagaagt
    45001 taaagcaaaa ttgcacgact taggtttagg tttacgtaaa gaagattaaa accttttacg
    45061 aaggagggaa accaacgtga gttatcgtaa actaggacgc acatctagcc aacgtaaagc
    45121 gatgttgcgt gatttaacaa ctgatttatt aatcaacgaa cgtattgtga ctactgaagc
    45181 tcgtgcgaaa gaagttcgct caactgcaga aaaaatgatc actttaggta aacgcggaga
    45241 tttgcatgca cgtcgtcaag ctgcggcttt cgtacgtaat gaagttgcca gcgtgcgtga
    45301 agaaaatgag gacatcgtca ttgaatcagc tttacaaaaa ctgttcaacg atattgcacc
    45361 tcgttacgca gaacgtcaag gcggatacac acgcattttg aagacagaac caagacgcgg
    45421 tgatggtgca ccaatggttg tcttagaact tgtctaaaat cacactaata acatcacttt
    45481 atagatgatt cttttagagt gttatgatgt tggaagatat ttttccgagt ctagctcagc
    45541 gctgctcctc aagcgaatat cgcttgggga gtaagcactc atcataaagt gtttttttat
    45601 accttcaatt acctgtcatg acagatatgt ttgaaggcat aaaaaaacat tttaagcgag
    45661 aaataattga aaattttgtc tttttttgat agtgttagta ggaactttag gatgaagaag
    45721 gagtaatcat ggagccaatt attgaattag aaaagataaa ttataaatac cagccagatg
    45781 atcttcgccc cgcattgaaa gatgtctcct ttacaattga taaaggtgag tggatcgcta
    45841 ttattggaca caatggttca ggaaaatcga cacttgccaa aacgatcaat ggattgcttc
    45901 tgccagaatc agggatcgtg aaagtgggaa atcaaatatt ggatgaagaa aatatttgga
    45961 cgatccgaca gatggttgga atgatttttc aaaatccaga caatcagttt gttggttcga
    46021 cagtagaaga tgacgttgct tttggtcttg aaaatcaagg gattcccaga gaagaaatgc
    46081 tagtgcgtgt gaaagatgca cttgagaaag ttagaatggc tgagtttgct tcacgcgagc
    46141 ctgctcgttt atcaggaggg caaaaacaac gtgttgccat tgccggcgtg gttgccttac
    46201 gacctgatat cattatttta gatgaagcaa ccagtatgct agatccagaa ggtcgtgaag
    46261 aagtgatctc tacgatcaag aagatcaaag aagaaagtca attgaccgtt atatctatca
    46321 ctcatgatat tgatgaagca gctaatgcca atcggatact agtgatgaga caaggcgaac
    46381 ttgtccgtga aggtacacca aaagaaattt tttctgctgg tcctgaactg atcgacttag
    46441 gtctagatct accatttcct gaaaaactga agagcgcact gaaagaacgt ggggttgatg
    46501 tgcccagtga atatatgaca gaagaaagga tggtggattg gttatggaca tccgttttga
    46561 acaagtagac ttcacttatc agccgaatac accatttgaa caaagagcct tatttgacat
    46621 caatatgacg atcaaagaaa acagttatac tgcattagta gggcataccg gaagcgggaa
    46681 atcaacctta cttcagcatt tgaatgcgct tgttaagcca acaagcggta cagtccatat
    46741 tggagaacga gatattcagc cggatacgga taataagaat ttaaagccca tccgaaaaaa
    46801 agttggcatc gtctttcagt ttccagaagc acagctattc gaagaaacgg tagcaaaaga
    46861 cattgctttc ggtcctaaaa actttggtgt cagcgaagaa gaagcactag tcctagcaaa
    46921 agaaacatta gaacaagttg ggctggatga aagctatttg gaacgttcgc catttgaact
    46981 ttcaggaggg caaatgcgta gagtagcaat cgctggtgtg cttgccatga ggccggaagt
    47041 acttgtattg gatgaaccaa cggcaggact tgatccccag ggcagaaaag aaatgatgga
    47101 gatgttttgg cgtcttcata aggaacagca gatcactatt gtattagtta cgcatttaat
    47161 ggatgatgta gccaattttg cagattatgt ctatgtgttg gaaaagggac gaatcgttaa
    47221 tagcggggag ccgcaagagg tctttcaaaa cattgaatgg ctaaaagaaa aacaactagg
    47281 cgtacctact gccaccgagt ttgcagaaga attaatggct aaagggatga actttgctac
    47341 cttgcctttg acagctgaag aattagcaga cgctatcgtc cattatgctg gaggaaatgc
    47401 ccatgatgaa taaattgata tttggtcgtt atatgcctgg agattccttg atccatcgat
    47461 tggatccccg agctaaatta ttagccagtt tttattttat cggaatcatt tttttagcaa
    47521 ataattggga aacgtacctt ttgttggcat ttttcacatt actttctgtc ttcttgtcaa
    47581 aagtagattt gaaatttttt atccgaggca ttcgcccatt gatctggcta atcctcttca
    47641 ctgtagcttt gcaggtcttg tttacaagtg gcggagaaac ttattggagt tggggtgttt
    47701 tccatatcac tgaatttggt gtgatgaacg gcctctttat tttctgtcga ttcgtgttga
    47761 tcatttttat gtcgactcta ctcacgctta ctacaccgcc tttggaatta tcggatgcga
    47821 ttgagtactt gcttcgtcca ttgaaagccg tacggttccc agtccacgaa atttcattga
    47881 tgctgtcgat tgctcttcgt tttgtgccta cattgatgga cgaaacagaa aagatcatga
    47941 acgctcagcg cgctcgcggt gttgattttg gtgaaggaaa cctagttcag aaaatgaaag
    48001 cagtcattcc tttgctgatt cctttatttg tcagcagctt caaccgtgcg gaagatcttg
    48061 cgactgctat ggaagcaaga ggctacaaag gtggggaagg aagaacgaaa tatcgtgtat
    48121 tgcattggca taatagagac acgatggtta tcattgcttt tgtattatta actgtgctac
    48181 tgatcttttt aagaggataa acgttcagtg ggccgctatc tattcttttc ttgctgatct
    48241 gtttgcaaga aaggatgcaa gcagtgtatg acaagaaata ttagggtctg tggcagacta
    48301 aggtcacagg cctctttctt ttatctgaaa agaaagaacg aaaaaatttt atagaaaaat
    48361 cattattcca gataaccttt taggaacaaa ggagagaaaa acatggtacg ttacaaagca
    48421 atcattgcgt acgacggaac aaattttaat ggattccaaa aacagccaaa tgggcgtact
    48481 gttcaagaag aagtagaaaa aacattacaa aaaatggcaa atggaaaaga aatcacggtc
    48541 tttggttcgg gaaggacaga tgccggagta catgcgatcg ggcaagtaat ccattttgat
    48601 tatccagagg agcgtccgtt agaacgaatg cgtttcgcgc tagataccca gtcaccagag
    48661 gatattgctg taagacaagt agaaattgtt tcagaagact tccacgcaag atatttggtc
    48721 aaagaaaaga cgtatcaatt tcgagtggat atagggaaac cacgcagtcc ttttcgacgc
    48781 cattatgcaa gttattttcc ttacccactt gatttggaaa aaattcagcg ggcgcttcct
    48841 gatttattag ggactcacga tttcacctct ttttgtgcat caggaagctc gatagaggat
    48901 aaaatacgta cgatctacga ggctaagatg gaggtaaatg aaacaaaaga cgaattgttg
    48961 tttacattta ggggaaatgg cttcttgtat aagatgatac ggatcttagt aggaacgctt
    49021 ttgaaaattg gcaatggccg attgcctgaa gatagtatcc cagatattat tgcgaaaaaa
    49081 gatcgtaatg cagcaggacc tacagcacac cctgaaggac tttatttgta tgaagtgaaa
    49141 tatgaataag aggttatgat agaagtggtc agcttcaaga aataagaagg tatttacgaa
    49201 aattacttct caaaattttg caaaatttcg gcttattttc gaagaagcta cttctggaac
    49261 accgtttaag aaagaggtcg tgagcctgtc atttaactct gagtcacaaa gagcaatttc
    49321 aaaaaacagc ttctcatctt tgatgaatat cgtgacttgt ggacgaggcg ttgataagtg
    49381 aatcatcttt ttgttaccta ttttttctgt aaccaaaaaa cgatggaacg ttgttttgat
    49441 tgcccttggt gcggacagtc tgaacaggtt ccatcgtttc tttattttct acgtcttctt
    49501 ctgtgtgtgt ttcgtggttg ttcttctatt gctggatcaa tcgtgccata aaaggggcga
    49561 ttgcggtcaa ggtaccagac ataaagaata aacaatgcaa tacataggac aaaaaggatt
    49621 gcacctatcg taaatccttc aggcaagtaa ctgaaagtga cagtatgttt acctgctgga
    49681 acttttatca tcaagaatgc gtctttgaaa ttgctgacct tgactggttt cccatcaatt
    49741 ttagctttcc atcctttgtc ataaggaata gttgtcacca acatggaatc ttcagcggta
    49801 tttacagtcc cagaagcttt tctgccagtg gcttccattt cgacaccttt ttctttgatc
    49861 tgattcattg cggcttgata ggctacaaca tctagaccaa cgactttagg ctccatgaaa
    49921 cttatttctt tcgtcccata aaaacttgca gtaaatgtca ctgttgtctc ttgaggataa
    49981 tagccaagat catagtattg accagaaatg ttgatttgcg tttttcgcga tgtaccattg
    50041 acagtgatcg tcgcactaga actttctaat tgcgcaaaat tggttgggaa caaacttaag
    50101 taagcttgtg tatttgcagg cacatggact tcccaagtga gctcttttgg ctggttatct
    50161 gctgtttctg agtaagtgac gccagtcgtt gtctttgtga tcgtcgtatt caactgattc
    50221 ttcaaagtag gtgtatagaa tgtaaaatac tgttcattta gaccagacaa tgtattgatc
    50281 aagtttgtct gactggtcaa attatctgtc actggctgct caacatcatt aacggacaac
    50341 ggtgcagtaa atgctagtgg caatgcattg acattctgat acagtgtata gtcgccgctt
    50401 tgaccagctt ggcggaagcc atatttactg aacgtgcttt cgtttttaga aatgttatat
    50461 ttgattcctg taaacccatc catcagcaaa gtattattgt tataacggat attcaaattt
    50521 gtccctcttg aacggaaacc taatttatcc aggtaagagg aagaattacg atttcttata
    50581 gaagaaaaca aactgatacc actgtaacca tagttgatcg aatcgtttgc agatactgga
    50641 tccagatttt ccaagcggta aaacgagtcg gaatcttttt ttgtttcatt tacaagagct
    50701 ttcaagtcag gataaggatc agtatataaa ctgcgggaag cataattcca atcatcaaga
    50761 atgccatgga tcatactgtt cgtattgacc agtgcttcag cagacatgat caataataac
    50821 aatatactca gatgattgaa tgaaagtttt ttatgctgat aaaatcccac agcaaaagca
    50881 taaatcccta agaaaaccac tgtcaataca aaggaagtca tcgtcacata ggtgtagctt
    50941 ccttcaggtt tgatgcccca tgccaccgca aagagagcag ctacgaccaa aatgattcct
    51001 aacaacagcc caccatcgtc ttgttctatc ttttcccatc cttttcctgc aagcaggagc
    51061 actaagaaag agaacagata ggcataacgg aataaaaaca tattcggtgc atgcatacca
    51121 tgccagaaca aattcaatgg aacaagataa aaacttgcga taagtatggc gaaaagactg
    51181 ccgtacaaaa atttattttt caacggtact ttccgactgg caaaataaaa cagacagagg
    51241 actaatggta gcagacctgc gtaaacgaac ggaatcgatc cgtattttgt cgtgtcataa
    51301 acaccaatca tatttttcat gattagatcc agataagaag tcgcttctgt tttcaacgta
    51361 gtgatctgac tcagtgcctc gccatttgac cgaagatcca gcactgctgg caatacaatg
    51421 atcattgaag cgccgcctgc cagtaaagaa gtgataccat aaggaacaat cgttttttta
    51481 tattctgacc agtttgtcaa tagacgggcg ataaaatata agaaagaaaa tacgccaatc
    51541 ataaaaccca tataaaaact ggataaaaac aagagtaaat aactgataaa taaaagtttc
    51601 ggccgtttaa gatccataac acgatgaatc cccaaaatca ctaatggaag ataaacaaat
    51661 gcatccagcc acatgatgat ctccgaatgt gcagtgataa atgagatcaa tgcgtaactg
    51721 acgctaagtg caacatgccc ccagcgagga atcttgaacg tacgtgcaag aaaccaaaaa
    51781 gccaatccgg cacagccgac cttaagcaaa gtcagaacat acagtgcatc tggcatcatc
    51841 tggtttggaa aaaagaacac cagtggcgtg aagatcccgc ctagatagta agaaattaaa
    51901 gataaataat tcaatcctag agaagcattc catgtataaa aaatactttg ctttccaaga
    51961 agaacatttc ggaagctggc atgaaaatta gaaaactgtg aaaaggcatc acttgccaaa
    52021 atacttctgc tgcttccagg atagattccg atacttagat aaacaagtgc taaaatcaaa
    52081 aacggaatca ggaaactagc tgctgcgtag cgtccgtttt ctctaaagaa tgactttaaa
    52141 ttttttttca tatttatttt ccaccaaatt tctatgaagt tgtattcagt ttatcatatc
    52201 cttgtttttc tctgcttaaa aagagtctag ttttcacaaa aaaataaaat ttagaataaa
    52261 taacttcata atttgttcgt ataacaaaaa taaacgcaaa aaaagggtag gaatcaaaag
    52321 tcccaccctt aaaaattttt attaaataaa aaaccgttca ctatctgaac ggtccaagta
    52381 tcattcaatt aaacgcgttc aacttttcct gatttcaaag cacgagttga cacccaaact
    52441 tttttaggtt ttccgtcgac cataacgcga actttttgta agttaggttt aacagtacgt
    52501 tttgttgagt tcatagcgtg tgaacggttg tttccacttt ttgtcttgcg acctgtaaag
    52561 taacaaactt ttgccatggt aatttcctcc tttgccttga tttggagcaa tagaataatt
    52621 cagctcatac cagactaaca tatcatatag aatgggaatt tgcaagtaca ttttcataga
    52681 tacaattgac aattgagaag aataattgta aattaaacaa ggttatacct gaaattttgc
    52741 aaggtttttg agtactttgg gtaactgcct tttatttcaa gttgcgctat gataaaatat
    52801 gatagaagaa aaatgggagt tttttacccg ttaaggaggc tcattcatgg ctgtaaaaat
    52861 tcaaacgtca gctggaacca ttgaaattac caatgatgta attgcaacag ttgttggtgg
    52921 cgccgcgaca gatgtgttcg gtgttgtagg aatggccagc aaaaatcaaa ttaaagataa
    52981 tattactgag attttgcgta aagaaaatta ctcaagagga gtagttgtcc gccaagaaga
    53041 taatggtatt gctgtagatg tttatacgat tatcagctat ggtacgaaaa tctctgaagt
    53101 ttcccgcaac gtccaagaaa aagtgaaata caatctagag gcattattag gcgtaactgc
    53161 caattcagtt aatgtctttg tacaaggtgt tcgtgtactg ccagactagg caggagactg
    53221 gctggtaact cagcagtcga tctgaaggag gattatctag gtgagtgtaa cagaaataag
    53281 tgcaagccag ttccaggaaa tggtccaggc aggtgcgaat cgtctgcaag taaatgcaga
    53341 atacgtcaat tcattaaatg tattccctgt gccagatggc gatacgggaa caaatatgaa
    53401 tctatctatg actagtggag ctaaggcagt agtagattca acctcagaaa aagtaggaga
    53461 attagcagca atgctttcaa aaggattgct gatgggtgcg cgcggaaatt ccggcgttat
    53521 tttgtctcaa ttgtttcgtg gattttctaa gcaaattcca gatgttgtct cattaaatgc
    53581 ttctgatctt gctgcagcct tcactcatgg agttgaaact gcgtataaag cagtaatgaa
    53641 accagtagaa ggaacgattt tgacagtagc tagagaagca gcgaaagctg gagaaaagaa
    53701 agcaaaaagt actgatgatg tcgttgaagt aatgacagct gttgtaaaag gagcaaagcg
    53761 tgctttagct aaaactccag acttgcttcc agtcctaaaa gaagtcggtg tcgtagacag
    53821 tggagggcaa ggactgctgt ttgtttatga gggcttttta agtgcattga atggagaatt
    53881 ccaagcggat gaaacatatg aaccttctcc agcagaaatg gatgatatgg tcaatgcaga
    53941 acatcatcgc agtgtccaag gtcaaatggc gacagaagat atccattttg gttactgtac
    54001 agaaatcatg gtaaaaatcg gagaaggacc tacagtcgat agtacattcg attacgaaga
    54061 attccgtaac tatttagatg gtataggaga ttctttactt gtggtcaatg atgacgagat
    54121 catcaaagtc cacgtgcata cagaaaaccc aggagaagtc ttgaattatg gacaaaaatt
    54181 cggttcatta gtcaaaatca aagtcgacaa catgcgcttg cagcatgaga cgattttgga
    54241 acacgatgaa gaagtgaacg agtttgcgaa aaaagaaata ccacgtatcc cgtatggtat
    54301 cgtagcgatc gctgctggta aaggcgtcca agagctgttt gaaagtttag gcgctaatta
    54361 tgttatcagt ggtggacaaa caatgaatcc aagtacggaa gacattgtta aagcgattca
    54421 agaagtaaac gcagataaag tgattgtctt gccaaacaat aagaacatct tcatggcagc
    54481 tgatcaagca gcagaagtag cagatatccc agtagcagtt gttccttcaa gaacgatttc
    54541 ccaaggattg acagctatgc tttcattcaa tgatcaagct tctttagaag aaaacaaaga
    54601 aacgatgaca gaaatgttgt ctagcgttgt gagcggtcaa gtcactcacg cgatcagaga
    54661 tacctcaatt gacggtgtag agatcaaaga aggagacttt ttgggcatga tcgatggaaa
    54721 aatcctgatc tctaacccag atatcacagc aacagcgtta agtacactag aacaaatgat
    54781 caatgaggac acagagatcg taacgatctt ggcaggtgat gaaggaacag ttgaacaagc
    54841 aaatgaattt gctgagaaac taacagaaaa acatcctgat ttggaaatcg agatccacca
    54901 aggagatcag cctgtctatc cttatctatt ttctgctgaa taatttctgt gtcagaatat
    54961 ctttctaaca cagtattcat tcataaaagg ccttcggtta gtgatcaggt atgatcgctg
    55021 accaagtcct ttttttgtta aaggagggag gaaaaattta tgcgttcttt agatgatcca
    55081 atcactatgc tggcaggagt cggagaaaaa agagcagcta gtcttgccag cttgggcatc
    55141 caaacgattg aagacttact gacttattat ccttttcgct acgaagatat tcaggaaaga
    55201 aatctaaacg agatccagga tcaagaaaag gttactttga aaggaatcgt agtctcgcct
    55261 cctgtgatga atcgatttgg ttataaaaaa agccgattgc aatttcgtat gatgcaagaa
    55321 catgatgtgt tcaacgtttc tttttttaat caaccttatc taaaagaaaa agtcatttta
    55381 tcggaggaaa tcgccgtata cggtaaatgg gatgcaaagc gtaaagcctt gaacgggatg
    55441 aagattttag gttctcaaac aatggatgac ttttcgccga tatatcacgt caacaaatca
    55501 attcgtcaaa cgacattagt tgatctaatc aaaaaagggt ttcaagaata cggcacattg
    55561 attgaagaaa atctgccaaa caaccttgtg gagaaatatc gactgcttga ccgagcttct
    55621 gctgtaagaa gtatgcattt tccaaaagac cacgaagaaa accatcaagc caaacgacgt
    55681 gtcgtttttg aagagttctt tttatttcaa atgcgtcttc aaggattgaa aaaagcagaa
    55741 aaagcagaaa cgaatggttt ggagatcctt tatgatgtcc aaaaattgaa acaatttact
    55801 caaaagctac cttttgaact gacaaatgca caaaaacgtg taacgaatga aatctgtcgg
    55861 gatcttcgca gtccgcagca tatgcagcga cttttgcagg gagatgtagg tagcggaaaa
    55921 acagtggttg ccgcgattgc gttatacgct accgttacag caggtttcca aggtgcgttg
    55981 atggtgccta cagaaatctt ggcacaacaa catatggaaa gcttgcaaca gctgtttgat
    56041 cccaatgaag taaccatcgc attgattacg gggtcaacga aagcaaaaga acgacgtgag
    56101 ctccttgaaa aactagagca aggggaaatt aacgtcgtga tcggcacaca tgcattgatc
    56161 caagatggag tcgaatttca gaatttaggt ctagttatta cggatgagca gcaccgtttt
    56221 ggcgtgaacc agcggaaagt acttcgtgaa aaaggctggc gaccagatgt cttatttatg
    56281 acagcgactc cgattcctag aacattagct attacagcat atggtgagat ggatgtatct
    56341 atcattgatg aacttccagc tggacgtatc ccgatcgaaa cacgctgggt aagaacacct
    56401 caattggaca gtgtactgga ttggacgaaa aaagaactgg caagaggaca tcaaatgtat
    56461 gtcatctgtc cgctgattga agaatcagaa gcattagatg taaaaaatgc agtggaaatc
    56521 tatgaaaaac ttcgtgaact atttgcacca aaatatgaag ttggcttgtt acatggtaaa
    56581 atgaagaatc aggaaaaaga agtaatcatg gaagccttca aagacaacca gatgcagata
    56641 ttagtttcga ctactgtaat tgaagtaggg gtaaacgtgc cgaacgcgac cgtcatgctg
    56701 atcatggatg cggatcgatt tggattggca cagctgcacc aacttcgtgg acgtgttgga
    56761 cgcggaagtg atgcttccta ctgtatctta gttgctaatc ctaaaaatga actaggtgtg
    56821 gaacggatga aaatcatgac agagaccacc aatggttttg tactaagcga aaaagatctg
    56881 gaattacgtg gtcctggtga agtatttggc ttccgtcaat ctggtctgcc tcaatttgcg
    56941 attgctgact tggtgacgga tggaaatgtt ttggaggtag ccagagaaga ggctgcagcc
    57001 ctttggcaaa taaaggattg gcagatgctt cctgagtatc agcctttagc gcaaagcctc
    57061 caaatagaaa atcaggataa tcgttatttc gattagcaga ctgtgctata ctttatactg
    57121 atgacggaaa aaggagtgga aacaatgaaa attgccgtag atgcaatggg cggagatcac
    57181 gcacctcaag caattgtaga aggcgtggct ttagcgcagc aagattttcc agaagtagaa
    57241 ttcttacttt acggaaaaga agcagaaatc aagaaatact taacaaatga aaaaaacatc
    57301 acgattatcc atacagacga aaaaatcaat agtgatgatg agccagtaaa agcaattcga
    57361 cgaaaaaaga ctgcatcaat ggtccttgct gcacaagcag tgaaaaacgg tgaagcagat
    57421 gctgtatttt cagcagggaa tacaggcgcg ctactagcag cagggttgtt catcgtagga
    57481 cgtatcaaaa atatcgaacg ccctggcttg atgtctacct tgccagtagt agggaaagaa
    57541 ggtgccggtt tcgatatgct ggatcttggc gctaacgcag aaaacaaacc agaacatttg
    57601 ttgcaatacg gtattttagg ttctttttat gcacgtaaag tccgaggtat cggacgtcct
    57661 cgtgtcgcac tcttaaacaa cggaacagaa gcaacaaaag gtagtgaagt gacgaaaaaa
    57721 gcctatgaac tccttcagaa tgaaacaagc ttgaatttta ttggaaacgt agaagctcgt
    57781 gagctactca atggtgtggc tgatgtagtg gtcacagatg gatttacagg caacgctgtt
    57841 ttgaaatcta ttgaaggaac agcaatgaat atgatgaatc tattaaaatc tgctattcta
    57901 aatgaaggga tcaaaggaaa aatgggcgct atgcttttaa aagacggctt acgttctttg
    57961 aaagcagaaa tggattattc caaacatggc ggtgccgttt tgtttggctt gaaagcacca
    58021 gtcattaaaa ctcatggagc aacaggcccc gatgctgttc gctatacgat ccgccaaatc
    58081 cacacaatgc ttgagacaga tgtagttgga caattagtcg aacaatttga aaaaagcgaa
    58141 gaatagtatt tggatatctg aagcgatccg ttccattgaa aaaaaggtgg acattcctga
    58201 aaaatgccag taaaattagc catggtgtag gtaattgaca agtgtatgat tcttggaggt
    58261 gaaaaagttg actcgcgaag aagtatttaa caaagtagcg aaaattattt caaatcattt
    58321 tgagatagat accgataaag tgacggatga attgaacatc aaagatgatt taaaagctga
    58381 ttcaattagt attatggaat tcgttttaga gcttgaagat gaattcggta cggaaatttc
    58441 agatgaagac gctgaacaaa ttgaaacagt cggtggcgca gtagattata tcagcagcca
    58501 tcttaaataa tttcaaataa aagcaaagaa ggataaaaag ctgcatgcag ctttttatcc
    58561 ttcttttttt aatctttttg acacgtaaaa gtcatttttc tgataattct ggcgataaac
    58621 agaaattatg ttgcaaagta aagcggtttt ctatataatt attaataatt cattacacaa
    58681 agataagaat aacctttgtt ataaaacgat ttattctgtg taatgtcctt ttttattaag
    58741 aaaaacttcc atttaggaag cagagagggg tattcgattg tccgataacc aattattaga
    58801 agttcagaac ctacatacag gattccgact caaagatgac tattacgatg cagtagatga
    58861 cgtttcattt actttagaca aaaacgagat tttagcaatc gttggggaat ctgggtgcgg
    58921 gaaaagtacc ttggctacta cgattatggg attacttgat cctaataaca caaaaattac
    58981 aggtgagatt ttatataacg atctgaattt aatcgacctg aacgaaacgc tctataataa
    59041 aatccgcgga aatgatatcg gaatgatttt ccaagatccg ttgtctgcat taaatcctct
    59101 catgagaatc gaagaccaga tcaaagaagg actttcctat cacacaaaga tgaatgcaga
    59161 acagcgtcaa gctcgtgcac tagaattgct agaacaagta gggattccta atcctgcgag
    59221 agttggacgc caatatcccc atgaactatc aggcggtatg agacaacgtg tcatcatcgc
    59281 aatcgcaatc gcctgcaaac ctccaatcgt cattgcagat gaaccgacga cagccctaga
    59341 tgtaaccatc caagcacaga ttcttgattt actgaaagac cttcaggaag aaacaaaaac
    59401 agggatcatc ttgatcactc atgacttagg agtcgtagca gaaatggctg atcgagtggc
    59461 agtgatgtat ggcggacagt tcgttgaagt agcaggtgta aaagaactgt ttgatcatcc
    59521 taagcatcct tatacacgct ctttactgaa ctctatacca caagaaggaa atcatgatgc
    59581 agaactccat gtgatcgaag gtgtcgttcc ttctctgaaa aacatgcctc gtaaaggttg
    59641 tcggtttgca ccacgtattc catggatacc agcaagcgat catgaagaag agcctatcct
    59701 tcatgaagtc gcaccgaacc acttggtacg ttgctcctgc tataagcatt tccactttag
    59761 agacgaaaaa ggagaggtgt agatgacaga attaatcaca atcaaagacc tgaaagttca
    59821 ttaccctatt cgcagtggat tcttcaatcg agtaacggat catgtatatg ctgtagatgg
    59881 tgtggatttt attatcgaaa aaggaaagac atatggttta gtgggagagt ctggctctgg
    59941 aaaatctact acgggaaaag ccgttgtggg attagaaaag gtaacagccg gacaaatcat
    60001 gtatgaagga aaagatgtaa cgaagcgaag caaccgcaaa aaaatgggtt ataacaagga
    60061 tgtgcagatg atcttccaag attcaatgtc cagtttgaat cctaaaaagc gagtgttgga
    60121 tatcattgct gaacccatcc gaaattttga acggttaagc gatcaagaag aaaagaaaaa
    60181 ggtaaaagga ctgctagata tcgttgggat gccggaagat gcgctatata aatatcctca
    60241 tgaattttca ggcggacaac gtcaacgtct aggagtagcc cgtgctgttg ctaccaatcc
    60301 aaaactgatc gtagccgatg aacctgtatc tgctctggat ctatctgttc aagcgcaagt
    60361 attgaatttc atgaaacgaa tccagcaaga atacggcctc agctatttat tcatttcaca
    60421 tgatttaggt gtagtaaaac acatgtgcga caatatcgca attatgtaca aaggtcgatt
    60481 tgtagaaatc ggtacaagag aagacattta taatgacccg cgacatatct atacgaaacg
    60541 tctgctttca gctattccgc aaatcgatgt agaaaatagg gaaagccaca aagaaaatcg
    60601 acgaagagta gaacaagagt atatccaaaa ccaaaaagaa tactatgatg cgactggtcg
    60661 agtctacgat ttgcgtacga tcacaactac acacaaagtg gcgttgaaag atggaggtgc
    60721 aagctaatgt ggaaaacgat tctgcgacgt gttttattga tgatcccgca agttattatt
    60781 ttaagtgttc tgatcttctt attagcccaa gcgatgccgg gggatccatt taccggtttg
    60841 atcaatccaa atcaggatcc ggcagtgatc gaacagatgc gacaagcagc tggcttgaat
    60901 gacccatggt atgtccagta cttccgttgg atcggaaatg ctttgcaagg cgattttggt
    60961 caaagtttct tatacaaacg ctctgttgct gacttgatcg gcgaacggat catcaacact
    61021 ttatatctat cgatcttgac agttgtcctt acttatttga ttgctgtgcc tctggggatg
    61081 cttgctggcc ggtatcaaaa ttcaatcttg gataagattg tcgtaatcta caacttcttt
    61141 agttttgctg ttcctttatt tatcttcggc ttgatcatgc tatttgtttt tggttatcgt
    61201 ttaggctggt tcccaacaag tggatcacaa acactaggtt tgcaaggtgg atttatcact
    61261 caatggatag accgtttgaa atatattctg ttaccatcaa ttacccaagc atttttggcg
    61321 actgccgtca caatccaata tctgcgaagt gaagtaatcg attcgaaatc gctcgatttt
    61381 gtcagaacag cccgttccaa aggggtaccg acgaataaag tattcagtcg acatattttc
    61441 cgaaatgcag cgttgccgat ggcttctcaa atggggtatg aaatcacttc gttgattgct
    61501 ggttctgtag ttatcgaaaa gatttttggt tatccgggag tcggaaaact atttattgat
    61561 tccatcggcc aacgggacta taccgtgatc acatccttag tattgatttt aggaatcgcg
    61621 acgctagtgg ggacattact ttcagatatc atcatgagta tcgttgaccc gcgtattcgg
    61681 attcaataaa accaatcaga aaggagaatg gatcgatgag tgaaaaaaac aatacagcag
    61741 ttcaagaaag tattcctcct atggggatac gaatgattgc tcgagaattt aaaaaagata
    61801 aaattgctat tttttcatta ggtttgctag tggttttatt attagtgatc tttatcggtg
    61861 cgatgctgac cgatcaagac aaagtgatgt atgttagtat cttcgacaaa tacgctgcac
    61921 caggaacagt ttcgacacaa ggacaaaagt tcattctggg agcagatgaa ggcggacgtg
    61981 atgtattagg acaattgatc ataggtgcta gaaactctgt tacgatcgga tttgcgatca
    62041 cgatccttac ttcgatcatc ggggttggat tagggatcat ctctggctac tacggaggga
    62101 tcattgacaa catcttgatg cggatcgttg attttatcat gattctaccg atcatgctga
    62161 tcattatcgt cttcgtatcg attatttcca gatacaatgt ctggtccttt atattcatca
    62221 tgagtgcatt ttattgggta gctaaagccc gattattccg aagcaagaca ttatctgaag
    62281 caagaagaga ttatgtcagt gcctcaaaaa caatgggaac caatgatttt aaaatcatgt
    62341 tccgcgaaat catgccgaat ctaagttcat taatcattac taatttgacg atgaactttg
    62401 cggcaaacat cggtatcgag acaactttga ctttcttagg tttcggcttg ccggcaaatg
    62461 tacctagttt aggtacattg atcggttatg caagtaacgg agaagtctta gtgaatcggc
    62521 aatggatctg gttacccgca tcaatcctta ttttagtttt gatgttgagt atcaattatg
    62581 ttggacaagc attcaagcgt tctgcagatg cgcgccaacg gttaggctag tatagaagcg
    62641 aagggggaaa taggaatgaa gaaaacgatt tttggatttg cagcgctgat gtctgctgtc
    62701 gttcttgcag catgcggggg cggaggtaca gatacctctg gaaacaactc tggaggcaac
    62761 ggcaataaca aagaggaatt aacttttcca attgcaacaa aaaataatga tgatgcaatc
    62821 gaagacggcg aattagaagc tgcggtcgca acggacagtc agtttaaagg attgttccag
    62881 tgggagtttt accaagattc atatgatgca gcttttatgg ctccttctca tgaaccgcta
    62941 tttgcaaacg atgacgattt cagaatcaca gacgacggag ctgcaacgct tgatctagat
    63001 gaagatacga aaaaagcgac aatcaaagta aaagacaacg tcaaatggtc agatggtgaa
    63061 gatgtaacgg cggacgatgt gattttccct tacgaaatca ttgggaataa agattacact
    63121 ggtatccgtt atgacgacac tttccgtaac atcgtaggta tggaagaata tcatgatgga
    63181 agtgccgaca caatctccgg aatcaaaaaa atagacgaca aaacagttga aatcgaatat
    63241 aaagaagtaa acccaagcat gcttcaatcg ggtggtggta tctggtcata tgcagcacca
    63301 aaacatacgt tagaaggtgt agcaatcaaa gatatggaat caagcgatca agtacgtaaa
    63361 aatccagtga cttttggccc ttattacatg agtaatatcg ttgcaggaga atcagtagaa
    63421 tacttgccaa acgagcacta ctggaatggt acgcctaaat tgaaaaagat cacgatgaaa
    63481 tctgtaccga tggcaagtgc aactgaagca ttgaactcta aattatatga tatgattttc
    63541 caaatgccaa cagatactta cgatacctat aaagaaattg ctggctatac aaacttaggc
    63601 cgtgaacaaa cttcttatac ttatctagga ttcaagttag gtaaatggaa tgcagataca
    63661 aagagtgtgg aatacaatcc agattcaaaa atggctgata aatcattacg tcaagcaatg
    63721 ggctatgcat tagataataa tgcagtaggt gaacgtttct atgcaggttt gagaagcaat
    63781 gcgacttcat tgattcctcc agtattccag tcataccatg atgatgagat gaaaggattc
    63841 acacaagatc tagacaaagc gaaaaaacta ttagatgatg ctggctacaa agatgtggat
    63901 gacgatggtt taagagaaga caaagatgga aataaactaa caatcaactt tgcttcgatg
    63961 tccggtgggg aaacagcgca accattggca gattactata tccaacaatg gaaagaaatt
    64021 ggtttggatg tccaatacac aacaggtcgt ctgatcgaat tcaattcttt ctatgataaa
    64081 ttagaaaacg atgatcctga aattgatgtt taccaagctg cttggaatac aggaactgat
    64141 ccaaatccaa gtggactatg gggaccaaat gctgcgttca actacactcg ttatgaatca
    64201 gaagaaaata caaaattgat caacgatatc agttcaagca aagcatttga tgaagagtac
    64261 caaaaagaag catatcaaaa atggcaacaa tatgcatttg aagaagcatt tgcgattcca
    64321 acgttgttcc gtaatgaagt attgccagtc aacgaccgtg tcaaagactg ggattggtct
    64381 tatgatgtac caaacccatg ggcaaatgtt tctgttacag cagattcacg ttcataatca
    64441 ctaaaaatga ccgaaaaagg gcgatgtcct ttttcggttt tttgatattt ttctgtttat
    64501 ttctctaata ttcaggtcaa ggcgctgatt tcatgacttc tccaataaac ggtgttcaaa
    64561 gtccagtttc tcggtattaa gtcaaattta tgcaaaaata tggaaagcca ttttcacaaa
    64621 attttttctt catactcgaa actaaacgaa tttttcacaa cttctcgtca atcactagga
    64681 ttattaaagc tttacttacc ttttgaccga accatttcaa atttgacaaa aaaccagtat
    64741 aataaggaag tatcgaaaaa atatccataa aaggaatgtg gaaaatggac aaccagctga
    64801 caaaagaact aaaagaaaac tataacatcg tttttcacga tctaaatttg cttgaacaag
    64861 cttttaccca ttcatcctat gtgaatgagc atcgcaatct gcaattatcg gataatgagc
    64921 gacttgaatt tttaggagat gccgttttag aactgatggt ttctcagtat ttattccgtt
    64981 tatatcctca tttgccagaa ggaaaattga caaaaacacg agctgcgatc gttcgcgaag
    65041 acagcttgtc gaaatttgcc aaagaatgtc actttgatca atatgttttg ttgggcaagg
    65101 gagaagagaa ttcaggagga cgtacgcgac cagcattgct ttgcgaccta ttcgaagctt
    65161 ttttaggcgc gttatatctt gaccagggat tcgacacgac ccatgcattc atcgaaaaag
    65221 tgatttttcc taaagtcaaa gcaggtgctt tttcacatga gatggatcat aaaacaaaac
    65281 tacaagaagt cttgcaaaaa tcaggtgatg tatcaatcga ataccgattg atcaatgaag
    65341 aaggtccagc tcatgaacga gtattttgga tcgaagttta tgtagatgat caattgattg
    65401 gcactggaca agggaaatcg aaaaaattag cagaacaagc tgcggcggaa aatgcattgg
    65461 cagcactgta aaagtaagtg agggaaagct ttgtatttga aacgaattga aatagccggt
    65521 tttaaatcat ttgcagacaa gacagtcatt gattttgaaa atagcgtcac agcagtggtt
    65581 ggaccaaatg gcagcggaaa aagcaatatt acagaggcta tccgctgggt attaggagaa
    65641 caatctgcta aaagtctgcg aggcggaaaa atgccggata tcatctttgc cggttccgat
    65701 acccgtaagc agctgaatat tgcagaagtc acagtgattt tagacaatag tgatcattat
    65761 ctgccattgg agtacaatga gatcagtgta acccgccgtt atcgacggac tggggaaagc
    65821 gagtttttca tcaataaaca aagctgcaga ttaaaagata tccaagaatt gttcttagat
    65881 tctggattag gaaaagaatc gttctcaatc atttctcaag gaaaagtaga agcgattttc
    65941 agtagtaaac cggaagaccg tcgtgggatc tttgaagaag cagctggtgt cttgaaatac
    66001 aagcagcgga aaaagaaagc agaacagaag ctttttgaaa cagaagacaa cctcagccgc
    66061 gtccaagaca tcatccacga attggaagaa cagttaactc ctttggccgc ccagagtgag
    66121 gcagcaaagg agtttttacg gctaaaagaa acactgactc aaaccgatgt ctctttgatg
    66181 gttgctgaaa tcaagacagc aaaaaaagat tgggacaaca aacaagcaca gcttgcgaaa
    66241 ttcaatctag aattaggcaa actatctgag tcgatccaag aacaagaatc cattttggcg
    66301 aagcagcgta aagaaaatgc gcaagccgat cgtctgatcg aaaaaaatca gcaagtatta
    66361 ttagatcttt cggaaaaact gaagcagaca gaaggacaaa aagatgtttt gcaggagcgg
    66421 acaaagcata cacaaaaaag ctcgcaagaa tatcaaactt cattagcaga agcacaaaaa
    66481 aaggttaaac attttgaaaa gcttcaagaa agcttgatga aagcagcagc agaaaaagaa
    66541 acagagatcc aaaaagctga agcaaatctc ataaaaacac agcaagaatt agaaaaatac
    66601 caaaaatcaa cgaaagaatt attggcagaa ttaagagatc aatatgttga tttgatgcaa
    66661 gaacaagcag ctgtaggaaa cgaactgaag tatctagaac gtcaatatat acaagaaaca
    66721 gcaaaaagca agcagacgtt agccaagcaa tcggaagtag aagcatcagt agaccgattg
    66781 atcttacaaa aagaagaatt gacacaaaag caggcgcagc taaaatcatc tttaactgaa
    66841 acaaaagaga agttagaaat gatccaacaa aacgggaaaa agttccaaga agcattagca
    66901 aaagaacagc ctaaaatgta tcaattaatg aatcaagtcc agcagctacg tgctcgtaag
    66961 aaaagccttc aagaaatcca agaaaactat tttggttttt accaaggggt tcgattggtc
    67021 ttgcagcata aacagcagct ttcgggtatt gtcggagcag tggctgaact gatcgatgtt
    67081 cctgcagatt tcacgttagc tatcgaaaca gctttaggtg gggcagccca gcatgtcatc
    67141 gttgaaaatg aaaaagatgc tcggcaagcc atcacgtatt tgaaacagca aagaggagga
    67201 agagcaacat tccttccact gacgacaatc aaaccaagac aattaccggc tcatatattg
    67261 acacaagcag cagctgtaga agggtttata gggattgcca gtgaacaagt cagttatccc
    67321 gatcaaatcc aaactgtggt tcaaaacctg ctaggaacga tccttttagc caaagactta
    67381 actagtgcca atgcaatcgc gcaaacgatc cgctatcaat atcgagtggt ttccttagaa
    67441 ggtgatgtga tgaatgccgg aggatccatg acaggtggag cgaataaacg aggaaaccaa
    67501 ggcagtttgt ttgtacaaaa tcaagaattg aaacagttga ctagtgaatt tgaagaagca
    67561 gataaacaac tccaagcaca agaaaaaaaa gttcaggagc tgcaacaaga aacagctcgt
    67621 ttagccgaag aacaagaagt tttgcgtaca cgaggcgaac aattacgttt tgaagaacaa
    67681 gaagcgacaa atcagctaca aaacatcata aatgaacttg aacgttttga aaaagaaaag
    67741 caaatctcaa cttttgagac acgtgaactg cagcagttta tcgaagatta cgaaaagcaa
    67801 acgaacgaat tgaaagacaa acaaacagat cttgaatcgc aacgacaaaa aatcgacgaa
    67861 gaaatcaaat cgttaagtca agaaagcgac cagatggaag ccagaagagc acaagtacaa
    67921 agtcaaaaag cacaagaaca agcagatctt gctgtcttga aagagcagtt taatcatctg
    67981 cagatccagc tccgcggtgc ccgagtccaa aaagcagagg caaccgaacg tcaagaagcg
    68041 atagaaaagc agttggcgac attgacagca gacttttctg atcatgaagt cacagaagaa
    68101 agtttggaaa aacaaattaa cgaattatct gctcagcgag aaacattaaa agccgagttg
    68161 gcaaaagcca aagagcagcg agatagaagg caaaaggaga tcgataagct tgaagcagtc
    68221 ttggcagagc gtaatcgtga gcaaaaagca cgactgtcgg aacaatccaa attagaagtc
    68281 caaaaagacc gagctgaaat gattttagat aatcatttga cctatctgca gtcagaatat
    68341 cagctgacat ttgagaaagc ttcccaagac tatcaagaaa caaccgatat cgaagacagt
    68401 agaacaaagg tttcatcctt aaaagaacag attgaaaaac tgggaccagt aaacttaaat
    68461 tcaatcgaac aatacgaaca agtcagtgaa agacatacat tcttagcaac acagcgagat
    68521 gatctgttag cagcgaaaaa ccaattgttt gaaacgatgg atgagatgga tgatgaagtg
    68581 cggacaagat tcaaagaagt atttgaagct atccgtcaag aattcaaaat tgtctttcca
    68641 aatatgtttg gcggcggtaa agctgaatta gtactgacgg aaccaactga tttgctgaaa
    68701 acaggtattg agattgaagt acaacctcct ggcaaaaaac tgcaaagtct aagtttgctt
    68761 tctggaggag aacgagcact tacagccatc gcgttgctct tttccattat ccgtgtctgt
    68821 cctgttccgt tttgtatctt agatgaggta gaagcagcgt tagatgaagc caacgtgaaa
    68881 cgttttggtc gttacttgag cgatttccaa gatgacacac aatttatcgt agtcacacac
    68941 cgcaaaggaa cgatggtcgc agcagatgta ctgtacggag tgacgatgca ggaatccggt
    69001 gtatcaaaaa tcgtttctgt acggatggaa gatatcaatg aagaggcgaa aatcaacgaa
    69061 acgaaagtgt aggaacgaaa atgatcaaat tagtagcaat cgatttggac ggtacattat
    69121 taaatgaaca aaagctgatt tcagatgaga ataaacaagc attagcacaa gcaaaacagc
    69181 aaggggtcaa gatcgttcta tgtacaggac gccccttagc tgcgatggcg cattatctgc
    69241 aagaattagg actggtagat gaaggtgact acagcatcac tttcaacggc ggtttggtac
    69301 aaaaaaatga tactggtgaa atcatggaaa agaaagtgat ggaagtatca gatatccaac
    69361 gcttatacaa actggctcag caattggatc tgccactaga tgttttgtct gacaatgtgg
    69421 ttttacagct gcctagtgcc ccaaggaagc aatccttgta caatgtgttg aataagctgc
    69481 tgcaattcca accagcggat ataagcgata ttacagatga atttgtgctt aataaggcag
    69541 taatcgctta tgaccaagaa gaattagatc ctaaaatcaa agaaatacca gcagaattcc
    69601 atgaaatgta tgagatcatc aaaacaagaa gtatgctttt ggaatttatg ccaaaaggtg
    69661 ttacaaaagc ttatggtatt tctttattgg caaaagatct tggtttgaaa caagaagaaa
    69721 tcatggcgat cggtgatgag gaaaatgatt taccgatgat ccaatatgct ggactaggcg
    69781 tagcgatgga gaatgctgtg cctttcgtca aagaagcagc agatttcgtg acagccagca
    69841 atgaagaaaa tggcgtagcc aaagtgatcc aaaaatttgt attagagtag ggggagtcaa
    69901 atgggatttt ttgataaaat caagcgtgct tttactggcg aagagaaaac agaagaagtt
    69961 gaagtacagg agaagtacga caagggttta gaaaagacgc gtaaaacatt tggtgaacgg
    70021 atgaatgagt tgtttgccaa ttttcgaaca gtcgatgaag acttttttga agaattagaa
    70081 gaaacactta tcggtgcaga tgtaggtttt gaaacagcga tacgaattac cgaagcatta
    70141 cgtcaagaag tgaaactcag aaatgcgaaa aaatcggcag aagtccaaaa tgcgatcatc
    70201 gagaaacttg tggatctcta tgaagcggaa ggaatcaatg aagtaaatga actaaatatc
    70261 caagaagacg gactaacagt tattttgttt gtcggtgtta atggtgtagg gaaaacaaca
    70321 agtattggta aacttgccca tcagttcaaa aacgaaggta aaaaagtatt gatggcagca
    70381 gcagatacct tccgcgctgg agcaatcgac cagttagttg tctggggcga acgcgctggt
    70441 gttgaagttg ttcgtggcaa tgcaggaggc gatccagcag ctgtcgtatt cgatgctttg
    70501 gaaagagcaa agacagagca ggcagatgtt ttactagttg atactgcagg ccgactgcaa
    70561 aataaagtga acttgatgaa cgaattagaa aaaataaaac gagtaatcca gcgggaatta
    70621 ccagaagcac cccaagaaac acttcttgta ttagatgcaa ccactggtca aaacgcgatg
    70681 gttcaagcaa aacaattcaa agaaacaaca gatgtgaccg gtcttgtttt gacaaaatta
    70741 gatggtacag caaagggcgg tatcgtactt gctatccgta atgaattgca tttaccagtg
    70801 aaattggtcg gtcttggtga aggaatcgat gacttggagc cgtttgatcc aaatgatttt
    70861 gtcgttggat tgttcaaagg attactaaaa gaagaataac ccatgtctaa aaagcaggag
    70921 ctgtgacaaa agttgcagct ccttgtttga atgattgttt ttaacatagc tgtgtttttg
    70981 atgccttcat ttttatactt atttgtaagc ggttctcaaa caatttcgtt ctttttctta
    71041 gactttttga ttataattag attcatatga attggaggtc acttaatgat gaattttaag
    71101 caattattat tgcatgtcaa tgcgcgccct tttatcgacc aagctcgatt tggtatagaa
    71161 cgggaaggac agcgggttga tcttgcagga aatctagcaa aaaccgatca tccagcaatc
    71221 tttggcgatc gatcctatca cccctatatc caaacagatt ttagcgaaac acaaacagag
    71281 atgatcaccc ctgttaccga ttctattccc gaattatttc agtatctcgc tgctgtttat
    71341 gatgtgactg ctcgttctat accgaaagaa gagatgatct ggccattaag tatgccacct
    71401 gccttaccgg aaaaagacga agagatcatt attgcaaaat taaaaaattt cgaagatgtc
    71461 ttgtatcgac gttatttagc aaaagaatac gggaagcgaa aacagatggt aagcggtatt
    71521 catttcaatt ttgaatttgg cgatgagttg ctaagaacat tgttcagcca tcaggaagaa
    71581 tttcaagatt tttccgaatt caaaacagaa ctttatttga aaacagcgag aaactttatg
    71641 cgctatcgct ggatgatcac ttatttattc ggtgcttcac caatgagtga gaagaactac
    71701 tttttagatg aatcacatcc gcaagaacct gttcgcagta ttcgcaacag tgcgctgggc
    71761 tatacgaatc atccaaatgt gaaagtttcc tatgcttcta tgaaacagta tttagcagat
    71821 atcgagcgaa tgatcgaaga agggaaactt tcggaggaga aagaatttta tacaccgctt
    71881 cgtttccgag gcggaaagaa agtcgcagat ttagcaacaa caggtgttcg ttatattgaa
    71941 ttgcggaata tcgatttgaa tccttatgca agattgggta tcaatccaga gcaagttcgg
    72001 ttcttacaat tattcctgat gtacatgtta tggacagaag aaaaagaaga ctgtgaccaa
    72061 tgggtagcag aaggtacgac tcgaaataac aaagtagcac tagaacaacc atcagatcaa
    72121 acagaatttc atcaagaagg tagagaaatt cttgaaggaa tgaagcaaat gctggttgaa
    72181 ttggattggt tggattctct ttatttagta gaggaagcat tgactcaaat ggatcatcct
    72241 gaacaaacgt tagcagcgaa actctatcaa gaagcacagc tatcgagtca gcaagaagtt
    72301 gctgtggcat tgggacatca atattataaa gaaagccatg aacgtcccta tcagttggcc
    72361 ggttttcgcg aaatggaact ttctactcag atatttatgt tcgatgccat ccaaaaaggt
    72421 gtccaagtca aagtattaga tgagtcggat caatttttgc gtctgcaatt ccaagaccat
    72481 gtagaatatg tgaaaaatgc aaacatgaca agtaaggaca gctatatcgt cccacttatc
    72541 atggaaaaca aaacagtaac gaaaaaagtc ttaaaagaag ctgggttccg ggtaccagga
    72601 ggcgcagaat tttcatccat ggaagaagcg gtaaaagctt atccgagatt tgccgagcag
    72661 gcatttgtga tcaaacctaa atcaaccaat tacgggttag gtatcacgat tttcaaggaa
    72721 ggagctagtc tggaagatta tcaagcaggt ttagccattg cttttcgtga agacagttcg
    72781 gttttagtag aagaatttat gccgggaacg gaatatcgtt tctttgtgat cgacggagaa
    72841 gtccaagcca tcatgttgcg agtccctgct aatgttattg gtgacagtat ccgaacagtg
    72901 aaagaacttg ttgaagaaaa aaatagtgac cctttgcgtg gaacgaatca tcgtgcacct
    72961 ttagaattga tccaattagg tgaattagaa caattgatgt tgaaagaaca agggttgacg
    73021 atcgaatcag ttccccaagc caatcaaatt gtttatctaa gggagaactc aaatatcagt
    73081 acaggtggtg attcgatcga tatgactgat gaattttctg aagcttacaa aaaaatcgcc
    73141 gtttctgcgg tagaagcact aggagctaag attagtggta tcgatttgat tataccagat
    73201 aaagaaatcg atcctacaac tgataaaaaa gcatatggga tcatcgaagc aaatttcaat
    73261 ccagccatgc atatgcatgt ttatcctttt gcaggaaaag gcagaagatt gacgatgaat
    73321 gtattgaaat tgttatatcc tgaagtattt taaaagaaaa agcttttgac agattgtcct
    73381 atttgatgtc aatctgatca aaagcttttt ttgcatgtaa aaatttatgt gttttttcgt
    73441 tttaaaataa acgatcccag aagctttttt tcttttttgg tttttctttt tcagctggcg
    73501 aagagttttc ttctgggtat tttttttcga tatcaatcac aggttcattt actgcttctt
    73561 ttgctgttaa gagcaatccg aattcctcag gatttgcagt gacgtaatcg ttgactacag
    73621 tgaaacggct attgtttttc gttgctagct ggatatattg gttttggaca gaaaggggta
    73681 gagccccatt caagagtatg gaagcatctg gatgtttttt gagttctttt aaaaagtttt
    73741 ctttgttttt tggttggatc atttcagcaa cagtcatact aagatagcag cgttccctga
    73801 atgtccctaa atatttatgc tgttcttcag ggttcactaa aggtgtacca taaagtccct
    73861 tatccaaatg gctttttaca tcatcttgtg ccataaaaaa taccctcctt cttctctttg
    73921 tctttcttac gattatagca tattcccaat aatacctaaa acatatgaaa aagcggttgc
    73981 ttttgttcaa cgattcactt aaaataagaa tgaaaatcat tctcaattag aaaggtggat
    74041 tggatgcgct acggacgact agtagcatta ttagttattt taagtagtat ttcattgttc
    74101 atcggagtat cgaatgtaca attgtcagaa ctgctccagt tagatgggca aagcttacaa
    74161 atcttgctag tgactcgttt gccacgaacg atttgtttga tccttgtggg agcgaccagt
    74221 agtatttgtg ggttgatcat gcaacatttg acccaaaata aatttgtttc accaacgact
    74281 gcgggcacga tggatagtgc gcgtttaggt attttagtgg ctatgatttt cctacctggt
    74341 gcatccttga ttgtccgatc cttgacagca ttttgctttg cttttgcagg aacattgctt
    74401 tttttatccc ttacgcgatt gttcctccag aaaaatcaat tgatgcttcc gttgattgga
    74461 gtcatgttag ggaatatcat tggttcagtt gctacttttt ttgcttatca attccagttg
    74521 gtacaaaata tgacttcatg gcttcaaggg aattttgcga ctgtgatacg aggaaattat
    74581 gagttattgt atgctgttgt tccattgttg ctcttggttt ggctatttgc ttaccgattt
    74641 acgatcattg gtctgggcga atcgtttgca gtaaatctgg gaatagatta ccagaagatg
    74701 cagtttttag gcattggctt agtatcattg gctagtgcag tcatgttgat catggtagga
    74761 agcattcctt ttatcggagt gatcattccg aatcttgtct ctagaatata tggagatcaa
    74821 gtgcacaaaa caattggcgt aactgcttta gcaggtagtt tgttcttagt attttgcgat
    74881 cttgtcgctc gcgtgttgat ttttccttat gaaataccag tcagtgtgat cgttggaatc
    74941 atcggtgggg ctatcttcct ttatcttctg ataaggggac gacgatatgc ttaaaaaaaa
    75001 gccatctatt tttattggta tgcttgttct tgttttattt gttacaggaa tgtacttgac
    75061 gatccataca tatggaaact ggcattttgc atgtgcattg aggagtaaga aaatttttgc
    75121 atttatcctt gtctcgctag ctgtcagcag tgccacaatc agctttcaga caattacgca
    75181 taaccagttt ttgactcctt cagtattggg acttgagtcg ttatatgtcc ttgtccaaac
    75241 ggtccttttc ttctttatcg gaggagtgac gatgcttcag cagacaggta cagttttttt
    75301 cttattgaac gttgggctga tggtcgggct gagtctattg ttatttttct ttcttttgaa
    75361 aaaaatgaga gcgaatcttt ttttattact gatgtcaggg atgattttag gtacgctttt
    75421 tggtagtgtc agtacctttt tacaagtcat catggatccg aatgaatatg atttgcttca
    75481 aggcagatta tttgcaagct ttggcaatat ccagagtcag tatcttttgc ctgcttcttt
    75541 gatcattatc ggctgctggt acctgttatt tcgatatcga catgtgctag acgttttcca
    75601 tctcggaaat gaacaagcag taaacctcgg gatagacaca gatcgttttg aatttttgat
    75661 tttatgttgt gtcagtcttt tgacagggac atcaacggct cttgtgggtc cagttacttt
    75721 tttagggttt attgtcgcaa atatcagtta tcgtgtcttt tctacttatc gtcatggtca
    75781 gctatttatc ggaagctttc tggtagctat gctgtttctg acaggaggac agctcgcagt
    75841 agagcagatt ttcggctgga atacaacagt gagtgtcgtc gttgaatttg tcggaggaat
    75901 ttattttgtc acaaaattga ttcgcgaaag gaagagtatc ctatgattga tttacaaaag
    75961 gtcaataaac tgtaccatgg aaaatcagtg gttaaagatg tacagttaac gattcctgaa
    76021 aagaaattga ctgcttttat cggtccaaat ggtgctggaa agtcaacggt gttgtcgatg
    76081 atcagtcgtt tgataccaaa agatacaggt gaaatctatt tagaccataa cgaagtaaag
    76141 gcttggaaat ccaatgaatt agccaaaaaa ttatcgatcc tacgacaatc gaatgaaata
    76201 aatgtccaaa tcaccgtgaa agaattagta gaatttggac gatttcctta tacgaaagga
    76261 aaattgacga caaaagacga ggaaatcgtt caggaatcac tggaacacct tgggttagaa
    76321 gaaatagcgg atcaatcgat tgacactctt tcaggcggtc aactccaacg tgcgtatatt
    76381 gcaatggtgc tagcgcaaga cacggattat attttactgg atgaaccttt gaacaatcta
    76441 gatatgaatt ttgcggttca aatcatgcag attttaaaaa atttagtaca agaaatcggg
    76501 aaaacaattg ttattgttct tcatgatatc aactttgctg caagttatgc agacgaaatt
    76561 gttgcaatga aagatggaca gttatttacg caaggaacga cccaagaaat gataaaaaaa
    76621 gatgtattgg atgaattata cgaaatggac atacggattt gtgagattga aggccgcaga
    76681 ttctgtcttt atttctaaaa ctaacattag gagagaagag atgaaaaaat tagtagtagg
    76741 tatcttgatt ctagcatcat ttggtttagc agcatgtgga aatagcaaca acaccagtca
    76801 ggcagacaca aaaaaaagca gcacacaaac tgaaactaca ttgacgataa cagatagtaa
    76861 tggagaccaa atcgaggtgc caaataatcc agaaaaagtc gtcgtattcg acaatggttc
    76921 tttagatacg atggatgctc ttggtgttgg agataaggta gttggagcag ctacaagcag
    76981 ccttcctgag tatctttcat cctataaaaa agtagaatca gcaggtggaa taaaagaacc
    77041 agacttagaa aaaattaatc agcttcagcc agatctaatc atcatttctg gccgacaaag
    77101 agattttcaa aaagatctaa gtcaaattgc tccgaccatt ttcctttctt tagatgcgaa
    77161 aaatccatgg gaatctttcc agcaaaatgt cactgcttta ggggagatct ttggtaagca
    77221 agaagaagct aaaactcaat tagaggaatt atctagtgca atcgatcaga cgaaaaagaa
    77281 agcagaagct actgacaaaa aagcactagt cacacttgtg aatgaagggc aattatcagc
    77341 ctacggatct ggttctagat tcggtttcat ccatgattta tttggctttg aacaagcaga
    77401 tgatcaaatc gaagcatcca ctcacggtca aagtgtttct tatgaatatg tgctggaaaa
    77461 aaatccggat atcttatttg tagtagacag aacaaaagct attggtggag acgacagcaa
    77521 agatgatatt tctgccaatg aactggtagc acaaacgaat gctggtaaaa atcagcagat
    77581 catctccctt gaaccagatg tctggtattt aagcgggggc ggtttagaat caatgaaact
    77641 gatgatcgaa gatgtgaatc aagcatttaa atagcataaa taacagagga ttaaactctc
    77701 atactatgta acaataatgt ttcttacatt cttaaatagc aagagcctat cctcaaagaa
    77761 gttatcaaaa accatccgta aaatcgtatt gatgatttta cggatggttt ttatttattt
    77821 gagaaatctt ttttcaacgt gaattcatta tctacatcat acgggttgat tctagaatgg
    77881 ggagtggcaa aaaatagtgt gatccatttt ttcagcatca agatcgcctc ctttgatttg
    77941 aatcaaaggt acaaagcgaa acttagataa aacttaaatt tagatatttt tattttgtac
    78001 cgatctcttc tttaaacgat gttccatctg ctgcttctcg gcaataagtc cactagacta
    78061 gaaaatatga gaagctattt tctgtccagc gttcttatta ctcaaagcaa atcgcagttg
    78121 tcacaatctc ttctaaacga tgttccatct gctgcttctc ggcaataagc ccactagact
    78181 agaaaatatg agaagctatt ttctgtccag cgttcttatt actcaaagca aatcgcagtt
    78241 gtcacaatct cttctaaaca atgttcaatc agctgacctt cggtattagc tcaaagcact
    78301 gatttcacaa cctctattta ggcatgtgtc tcagcaagcg gttgatagtt gatgatctgc
    78361 atttttcgat aaaaatcggc gggaagctta ctccaccgtt ttaataattg atagaaataa
    78421 ctctcttctg ctttgatttt atactcgctt aatatcaatc caaaactatt cgtttttttt
    78481 gaagctaatt ttttataaat atccaatgct ttggttttat cttcattgac aaagagtgtt
    78541 tttacttcaa tataagaacc ctcgatattt tgtgtttctt cgataaaata gatgtttttt
    78601 atatattcct tcatagtgat tcctccaaag aagtctttta tttgataagt ttactctacc
    78661 gaagagttgt taataatccg tgaacaaaaa gtaattcttt ttccaatagt tgttaaaggt
    78721 tttttatttt ttatagtatt ttttgttatt taagaaagga ataaggttaa acaaataaca
    78781 ggttgctttt tgggtctttt tccgttcaag aaactgaacg aagaaaaggg aaagttgtct
    78841 cttttgtgat aagagaaatc ataataaacg aatgacttac tttccaataa attgagaaca
    78901 gtcagaacca gacaaattag ttgcatctga tgaataaagt gattattcta tgacatgagg
    78961 tgatacagat gaaattagaa gtgacaaaag aagcacaaga agtattgaag aataaattag
    79021 aagcagacga tcaactttta ttagatatcg aaaacggtga cggtccattt gcagaaagcc
    79081 aagtcagttg ccaattggat acggcatttc gattgatcat tgtcaaaaaa gatacacaaa
    79141 cagatctaag tctttattct gtagtggctg atacaccagt tggaccaatc aaaatcaaag
    79201 acagtgcaat actttacatg gatgatccat ccacacttgc tttagaacca acttataata
    79261 gtttacaatt aaaaggacca agcggcttga gaaaaggcaa cttgcaagtt gttcgaaaag
    79321 actagaggga taaaatggga aaattggcga tcatcagcga cctacatgtg gacatcaata
    79381 aattcggcga atcagagctg atgcagcttt ggcaagtttt gcaaaagcac aagatcactc
    79441 ggcttcatct agccggagat acggctaata aagtagaccg ttgcctagct gttgtcgaat
    79501 ttttcgtcaa taaaggatta tctacgacat ttcatttcgg caatcacgag ttggcagatg
    79561 tgcccaatga acagatgatg gaccattttc cagacccaca ttttttaaat gaacggtata
    79621 ttgctttaaa tgagcagacc gttcttttag ggatgaatgg ctggtacgac tatcagtttt
    79681 ccgaattgaa agattacgag gcaatcgtac gaatgaagcg tttgtattgg tatgatcggt
    79741 tcatccagcg tgggaaaaca gatctcgagg tcaatgcaga aatgcttgca gctgcaaaaa
    79801 atttgctaga ccagctggaa gcaaaaaaac ttgaggtgat tttatccact catttcgttc
    79861 ctaaacgggc atttatcatg tatcaatctg ctccttatga gcgttggaac aaattaaatg
    79921 catttttagg atcagaatca ttcggagaat tacttgaccg ttattcaaat atcaaacaag
    79981 tagtttttgg ccatacgcat cgtcgctttg aagataaatc catcggtcac acgatttaca
    80041 gctgcagacc ttttggttat tattatgaat ggcagctgac ccgagatttt gtcttgaaag
    80101 aacagttgct agacagctat gatcctatga aattacgtgt cctcttgcgt gcccatcacc
    80161 cagcttttca ggcctatcaa acacaacatt tgcaagagga atttgaacaa gcgatgacaa
    80221 tcgttccgta ttgagacagc attagtaatg tgtaaaagaa aataccttgt aaaacataag
    80281 gataagtaag agcagtagat ccatttatcg tggatatgct gctttttttg cggaaaataa
    80341 tcagcgaatg tttatttgat cggatgattt gtaactcgca gagtactaga tgcaagcgtg
    80401 tattcttttt ttgaacgata atcacaaaaa aaaata
//
LOCUS       DAJSBL010000005        72629 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.005, whole genome shotgun sequence.
ACCESSION   DAJSBL010000005 DAJSBL010000000
VERSION     DAJSBL010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 72629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 72629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..72629
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.005"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..791)
                     /locus_tag="ONT85_000440"
     CDS             complement(<1..791)
                     /locus_tag="ONT85_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HCR2377440.1"
                     /translation="MFPFFKKKPLKLQNTIILVVCFSTFVSLIVSAILIRNFILSMNR
                     SSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIMIASNLDFIVVMDPEGIR
                     YSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFRYFVPIYYDQEIIGAVCV
                     GLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMKKVLLGLEPQEIAEQLSE
                     KTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDFEVGK"
     gene            966..1490
                     /locus_tag="ONT85_000441"
     CDS             966..1490
                     /locus_tag="ONT85_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HCR2377441.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            1506..3005
                     /locus_tag="ONT85_000442"
     CDS             1506..3005
                     /locus_tag="ONT85_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HCR2377442.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(3093..3695)
                     /locus_tag="ONT85_000443"
     CDS             complement(3093..3695)
                     /locus_tag="ONT85_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HCR2377443.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            3799..4755
                     /locus_tag="ONT85_000444"
     CDS             3799..4755
                     /locus_tag="ONT85_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HCR2377444.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            complement(5060..6016)
                     /locus_tag="ONT85_000445"
     CDS             complement(5060..6016)
                     /locus_tag="ONT85_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR2377445.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            6190..6750
                     /locus_tag="ONT85_000446"
     CDS             6190..6750
                     /locus_tag="ONT85_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2377446.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            6743..7567
                     /locus_tag="ONT85_000447"
     CDS             6743..7567
                     /locus_tag="ONT85_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HCR2377447.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            7577..8194
                     /locus_tag="ONT85_000448"
     CDS             7577..8194
                     /locus_tag="ONT85_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HCR2377448.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            8212..8895
                     /locus_tag="ONT85_000449"
     CDS             8212..8895
                     /locus_tag="ONT85_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCR2377449.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            9578..10813
                     /locus_tag="ONT85_000450"
                     /pseudo
     CDS             9578..10813
                     /locus_tag="ONT85_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
     gene            10821..11708
                     /locus_tag="ONT85_000451"
     CDS             10821..11708
                     /locus_tag="ONT85_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCR2377450.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            11725..12561
                     /locus_tag="ONT85_000452"
     CDS             11725..12561
                     /locus_tag="ONT85_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCR2377451.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            12564..13187
                     /locus_tag="ONT85_000453"
     CDS             12564..13187
                     /locus_tag="ONT85_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2377452.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQPDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            13184..14476
                     /locus_tag="ONT85_000454"
     CDS             13184..14476
                     /locus_tag="ONT85_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR2377453.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     regulatory      14835..14932
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            15048..15629
                     /locus_tag="ONT85_000455"
     CDS             15048..15629
                     /locus_tag="ONT85_000455"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HCR2377454.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     gene            15632..16939
                     /locus_tag="ONT85_000456"
     CDS             15632..16939
                     /locus_tag="ONT85_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HCR2377455.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            17232..17720
                     /gene="purE"
                     /locus_tag="ONT85_000457"
     CDS             17232..17720
                     /gene="purE"
                     /locus_tag="ONT85_000457"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HCR2377456.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            17713..18837
                     /gene="purK"
                     /locus_tag="ONT85_000458"
     CDS             17713..18837
                     /gene="purK"
                     /locus_tag="ONT85_000458"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HCR2377457.1"
                     /translation="MVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVSDCP
                     AAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTDLLA
                     ITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKGQYV
                     LKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHNNIL
                     HETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRPHNS
                     GHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENKPDW
                     QFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            18850..20145
                     /gene="purB"
                     /locus_tag="ONT85_000459"
     CDS             18850..20145
                     /gene="purB"
                     /locus_tag="ONT85_000459"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HCR2377458.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(20325..20702)
                     /locus_tag="ONT85_000460"
     CDS             complement(20325..20702)
                     /locus_tag="ONT85_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2377459.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            20958..21686
                     /locus_tag="ONT85_000461"
     CDS             20958..21686
                     /locus_tag="ONT85_000461"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HCR2377460.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            21686..21940
                     /gene="purS"
                     /locus_tag="ONT85_000462"
     CDS             21686..21940
                     /gene="purS"
                     /locus_tag="ONT85_000462"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HCR2377461.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            21942..22613
                     /gene="purQ"
                     /locus_tag="ONT85_000463"
     CDS             21942..22613
                     /gene="purQ"
                     /locus_tag="ONT85_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HCR2377462.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            22614..24836
                     /gene="purL"
                     /locus_tag="ONT85_000464"
     CDS             22614..24836
                     /gene="purL"
                     /locus_tag="ONT85_000464"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HCR2377463.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            24821..26260
                     /locus_tag="ONT85_000465"
     CDS             24821..26260
                     /locus_tag="ONT85_000465"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HCR2377464.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            26283..27335
                     /locus_tag="ONT85_000466"
     CDS             26283..27335
                     /locus_tag="ONT85_000466"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HCR2377465.1"
                     /translation="MNSMANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGF
                     GGCFDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEP
                     LYFLDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFA
                     VGIAEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKK
                     TLGEELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSW
                     PILPIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVEESKVKEIVHEIEQAGEKVYEIGK
                     VIAKEKHRVVFIGEER"
     gene            27332..27910
                     /gene="purN"
                     /locus_tag="ONT85_000467"
     CDS             27332..27910
                     /gene="purN"
                     /locus_tag="ONT85_000467"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HCR2377466.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            27907..29442
                     /gene="purH"
                     /locus_tag="ONT85_000468"
     CDS             27907..29442
                     /gene="purH"
                     /locus_tag="ONT85_000468"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HCR2377467.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            29458..30732
                     /gene="purD"
                     /locus_tag="ONT85_000469"
     CDS             29458..30732
                     /gene="purD"
                     /locus_tag="ONT85_000469"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HCR2377468.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            30854..31768
                     /locus_tag="ONT85_000470"
     CDS             30854..31768
                     /locus_tag="ONT85_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HCR2377469.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(31901..33466)
                     /locus_tag="ONT85_000471"
     CDS             complement(31901..33466)
                     /locus_tag="ONT85_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HCR2377470.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(33459..34910)
                     /locus_tag="ONT85_000472"
     CDS             complement(33459..34910)
                     /locus_tag="ONT85_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HCR2377471.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITA
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(34924..35940)
                     /locus_tag="ONT85_000473"
     CDS             complement(34924..35940)
                     /locus_tag="ONT85_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR2377472.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            36160..37248
                     /locus_tag="ONT85_000474"
     CDS             36160..37248
                     /locus_tag="ONT85_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HCR2377473.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            37302..38828
                     /locus_tag="ONT85_000475"
     CDS             37302..38828
                     /locus_tag="ONT85_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HCR2377474.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            38830..40035
                     /locus_tag="ONT85_000476"
     CDS             38830..40035
                     /locus_tag="ONT85_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCR2377475.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            40292..41269
                     /gene="guaC"
                     /locus_tag="ONT85_000477"
     CDS             40292..41269
                     /gene="guaC"
                     /locus_tag="ONT85_000477"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HCR2377476.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            41533..42957
                     /locus_tag="ONT85_000478"
     CDS             41533..42957
                     /locus_tag="ONT85_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HCR2377477.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            43418..44527
                     /gene="pdhA"
                     /locus_tag="ONT85_000479"
     CDS             43418..44527
                     /gene="pdhA"
                     /locus_tag="ONT85_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HCR2377478.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            44530..45507
                     /locus_tag="ONT85_000480"
     CDS             44530..45507
                     /locus_tag="ONT85_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HCR2377479.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
     gene            45532..47175
                     /locus_tag="ONT85_000481"
     CDS             45532..47175
                     /locus_tag="ONT85_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HCR2377480.1"
                     /translation="MAYQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAAESQTPAKPAA
                     EPTVDTESAGSSSEGVFQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSAEAPKEKVETS
                     GSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKEDIENFLAGGPS
                     SAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPTRKAIAKAMVN
                     SKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALTATVKKYPVLN
                     ASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEINEKAKLAHDG
                     KLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIVNAEGEIVVGR
                     VMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            47183..48589
                     /gene="lpdA"
                     /locus_tag="ONT85_000482"
     CDS             47183..48589
                     /gene="lpdA"
                     /locus_tag="ONT85_000482"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HCR2377481.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            48751..49026
                     /locus_tag="ONT85_000483"
     CDS             48751..49026
                     /locus_tag="ONT85_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HCR2377482.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            49032..49799
                     /locus_tag="ONT85_000484"
     CDS             49032..49799
                     /locus_tag="ONT85_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HCR2377483.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            49967..51796
                     /gene="typA"
                     /locus_tag="ONT85_000485"
     CDS             49967..51796
                     /gene="typA"
                     /locus_tag="ONT85_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HCR2377484.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            52057..53829
                     /gene="recQ"
                     /locus_tag="ONT85_000486"
     CDS             52057..53829
                     /gene="recQ"
                     /locus_tag="ONT85_000486"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HCR2377485.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            53943..54245
                     /locus_tag="ONT85_000487"
     CDS             53943..54245
                     /locus_tag="ONT85_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HCR2377486.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            54248..55411
                     /locus_tag="ONT85_000488"
     CDS             54248..55411
                     /locus_tag="ONT85_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HCR2377487.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            55437..58865
                     /locus_tag="ONT85_000489"
     CDS             55437..58865
                     /locus_tag="ONT85_000489"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HCR2377488.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            58886..60010
                     /locus_tag="ONT85_000490"
     CDS             58886..60010
                     /locus_tag="ONT85_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2377489.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            60000..60437
                     /locus_tag="ONT85_000491"
     CDS             60000..60437
                     /locus_tag="ONT85_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HCR2377490.1"
                     /translation="MIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESAKVC
                     QLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQLQS
                     LLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            60421..60759
                     /locus_tag="ONT85_000492"
     CDS             60421..60759
                     /locus_tag="ONT85_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HCR2377491.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            60878..61438
                     /gene="rsmD"
                     /locus_tag="ONT85_000493"
     CDS             60878..61438
                     /gene="rsmD"
                     /locus_tag="ONT85_000493"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HCR2377492.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            61435..61926
                     /gene="coaD"
                     /locus_tag="ONT85_000494"
     CDS             61435..61926
                     /gene="coaD"
                     /locus_tag="ONT85_000494"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HCR2377493.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            61916..62959
                     /locus_tag="ONT85_000495"
     CDS             61916..62959
                     /locus_tag="ONT85_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HCR2377494.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            63118..63846
                     /locus_tag="ONT85_000496"
     CDS             63118..63846
                     /locus_tag="ONT85_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HCR2377495.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            63962..64207
                     /locus_tag="ONT85_000497"
     CDS             63962..64207
                     /locus_tag="ONT85_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HCR2377496.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            64204..64422
                     /locus_tag="ONT85_000498"
     CDS             64204..64422
                     /locus_tag="ONT85_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCR2377497.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            64558..65097
                     /locus_tag="ONT85_000499"
     CDS             64558..65097
                     /locus_tag="ONT85_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HCR2377498.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            65090..67363
                     /locus_tag="ONT85_000500"
     CDS             65090..67363
                     /locus_tag="ONT85_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HCR2377499.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            67401..68447
                     /gene="holA"
                     /locus_tag="ONT85_000501"
     CDS             67401..68447
                     /gene="holA"
                     /locus_tag="ONT85_000501"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HCR2377500.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(68536..68790)
                     /locus_tag="ONT85_000502"
     CDS             complement(68536..68790)
                     /locus_tag="ONT85_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HCR2377501.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(69190..69459)
                     /locus_tag="ONT85_000503"
     CDS             complement(69190..69459)
                     /locus_tag="ONT85_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2377502.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            complement(69929..70384)
                     /locus_tag="ONT85_000504"
     CDS             complement(69929..70384)
                     /locus_tag="ONT85_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HCR2377503.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            complement(70522..72450)
                     /locus_tag="ONT85_000505"
     CDS             complement(70522..72450)
                     /locus_tag="ONT85_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HCR2377504.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
BASE COUNT        23651 a        12522 c        15835 g        20621 t
ORIGIN      
        1 ttttttccta cttcgaaatc caatagctgc tctgtttggt agaaaatgtt ttttgcaggt
       61 gtattgatca agaggatagt tttatcagaa gagatggcta agattccttc atctacttca
      121 ttttcgataa tcgttttttc acttaactgt tctgctattt cttgtggttc aagtccaagc
      181 agtacttttt tcattttttg ggcaagaaaa acggcgccta tgatgcctac gctaagtcca
      241 ataatcaatg aaaaaacaat cattttttgt ccatctttga cttgattgtc aatcgtgttg
      301 tttgttaagc caacacaaac tgcaccaata atctcttgat cataataaat agggacgaaa
      361 tagcggaagc cgaaccctaa cattccttct ttttcagaga aaaattcttg tcctccaatc
      421 acttttttca catcttcatg acttgagaaa ggaagaccga tgtaacttgg gtctgcgtga
      481 gaatatcgaa tgccttcagg gtccatgaca acgataaaat ctaaattcga tgcaatcatg
      541 atttcatttg ttattccttg aatcgcatgt gtcgggtcac cagtctgcag ctttttttgg
      601 atgagtttat cattggcaac gattttagca atgctagaga ttttatctga cacactagat
      661 cggttcatcg aaagaataaa attcctgatc aatatggcag aaacaatcaa agaaacgaat
      721 gtggagaaac aaacgacaag gatgatcgtg ttctggagct ttaatggttt tttcttaaaa
      781 aaaggaaaca taggctacct cgaaagaatt tattgtgaaa attttaacat gaaaacaaaa
      841 atttaacaaa ttgtcaacga taaacacaac cttcctataa aaaacaccac tgagtcggta
      901 aaagaagcta tacagaattt tcaaacattg gtatgataga cgtagaaatg gaaggaggcg
      961 tgtatatgat agggggaatc gcacttgaga cgatttattg gtatacattg ctgatttgtg
     1021 caggattggc agtcctattg attatttttg gagatgtatt cgattttgat ggcccgcttg
     1081 atccgatgct tattatccct tggttaactt tcacaagctt atttggtttt ctaggtgagc
     1141 gtttaacaga tcaatcatct ttatttttat ttattgctag tggaggcata tcgatagtgt
     1201 tggtattttt gcttaatttc tatatattga tgccgatgaa acatgccgaa tcaacgatgt
     1261 cgtcttctga aaaaactctg gaaggacaag tagctgttgt gatcacaccg atccctgtac
     1321 gaggtatggg agaaattcag ttgaaaagtg ttaccggttc cattagtcgt ccagcttgtt
     1381 tttacagccc ccaagaacag acagctgagc ggggaacgaa agtattgatc attgagatca
     1441 aggaaagaat ctgttatgtc atgccttatg aaagcatgat aaaagcttaa aggagcgtgt
     1501 gttaaatgga aataccagtt gtttttatcg cgatcgcaat cgcagctttg attttactga
     1561 tgttgctgat cgtctttgta gcgaagtacc agacagcaaa accggatgaa gcgttgatca
     1621 tcagtggaag ttatttagga agcaaaaatg tgcacattga tgaaagcaat aacaaaatca
     1681 aaattgtgcg tggaggtggc gcattcgtct tgccagtttt tcaacgttcg aatcggatca
     1741 gtctattatc gagtaaattg gacgtctcca cccctgaagt gtataccgaa caaggcgtac
     1801 cggtgatgtg tgatggtact tcgatcatca aaatcggttc atctgtagaa gagattgcta
     1861 cagcagcaga acagttttta ggcaagacaa gagaagaact ggaaaatgaa gcaagagaag
     1921 tattagaagg acacttgcgc tcgattcttg gttctatgac tgtagaagaa atctatcaaa
     1981 atcgcgataa gttcagtcaa agcgtacaag aagtcgcttc tgtagacctt gcaaaaatgg
     2041 ggttgatcat cgtttctttc actatcaaag aggtcagaga caaaaacggt tatcttgact
     2101 ctttagggaa accaagaatc gcccaagtca aaagagatgc agatatagca gaagcggaag
     2161 ctctaaaaga aacgcgtatc aaaaaagctc aagcagaaaa agaatcacaa gcagctgaat
     2221 tacagcgaca aacagagatc gcagaatcat taaaagaaaa agaactgaaa ttagcaacct
     2281 acaaacaaga acaagatgtt gctaaagcaa aagcagacca agcctacaat ttagaaagtg
     2341 cccgagcaca acaacaagta atcgaacagg aaatgcagat taaaatcgta gaacgccaaa
     2401 aacaaatcga actggaagaa aaagaaatca cacgtcgtga aaagcaatat gattcggaag
     2461 tgaagaaaaa agcagatgcc gatcggtacg ctaaagaaca agaagcacaa gctcaaaaag
     2521 tcaaagaagt gactgaagcc gaagcagaac gattccgtgt ggaagcactg gcagaagcgg
     2581 aagccaataa gacacgttta gctggacaag cagaagccga agcagcacta gcaaaaggaa
     2641 aagcagaagc cgaagcaaaa caaaagatcg ccaatgcctt caaggaatat ggcgaagcag
     2701 ctgtcttgag tatggttatc gatatgctac cgcaattgat gcgcgaagca gcacagccgc
     2761 taggaaatat cgaaaaaatc tctgttgtcg atacaggaag cagttcagga gaaaccagtg
     2821 gagcgaaccg agtgacaaat tatgccacaa atttgctttc ttctactcaa gaaacactaa
     2881 aagcgacaac aggacttgac ttgaaggaaa tcgtagagaa tttgtcgaca cgtgggacta
     2941 ctagccaagt aaatatgtat gaagcgccaa aagaggcaga acaaaactcg atgaaggaag
     3001 aataaagtag tacacttcac cgttcaatca aaaatggagg aagacttttt aaagttcttc
     3061 tctccatttt tttattttat tgcaaaagaa aattagtcct cgctattttt cttttgtttt
     3121 tggatcttag cttctatctc ttgtaaattt gttcttagca atcgttcttt cttttggagt
     3181 tcgaccaaat cttctgatag ttcttccttc ttttctgttt caggaggacg aagaaagaaa
     3241 ttgataccga ttgcaatgaa tgtccctata aaagtatcta gtacacgttg gatagcatac
     3301 aaagcggatt cgccttgagg gatgctcaac gcaatcagca acaacgtagc tattgcggaa
     3361 atgataccgg aattattgtt aataccatct gacacgacga tcacaataat aactagaata
     3421 ggaagcaaga tcaattctac caaaaaatca ttgtgaaaat agcttttgac aaaaaaataa
     3481 atgatagcta atgtaccacc taatgtattg ccgatgatcc tagactttcc aaaagaaacg
     3541 gttgtagtca agtcctgacg caaagaaaaa acagcagata atgcagcaat catcgggatt
     3601 cctcgttgag tcaaatgaaa aaacaagata cataaaaaaa cagctaaggc acttttgatc
     3661 gtacgcatac ctagtcgaaa acggccaaat tgcatagaga ttctcctttt tggtcttttt
     3721 actatcatac ctaaactttt gtctgatagc tagtttttca ttattcaagt ataattaaaa
     3781 taaaaaaggg ggaagtaaat gcgtatagga atgcgaacag tcaaaacagt gatcagtgtc
     3841 ttcttttcga tggtcacagc gggtagctta tcgctgttgt attggccagc tgcagggatc
     3901 attgcattat taagtgtagg aaataccaag cgctctacat tgcgtacggg catgaatcga
     3961 atcgcagcct ttatattagc tacggcaaca gcactgttca gtttttttgc tgttgggtat
     4021 actatttttg gcttcagtct ttttttattc ctgttcatcc cgcttgcctc tcgcttcaaa
     4081 ttaaccgaag ggatagtagt aaattcggtt ttggtcactc attatttagt ggaggaaaac
     4141 atgtcttggc agctgatcgg caatgaagcg ggattaatgg cgattggatt agtatttgct
     4201 ttgcttgcaa atgtttacat gccagatcgt acgaagcagc tgaaggaaaa ccaaatacag
     4261 atcgaaaaag aatttcgaaa tattttgaga caaatggcag aatttctttt atcagaaaat
     4321 aaagaagatg tgcagatcaa atgcgaacat ttactgacat tcattcgcga aagccaagaa
     4381 gatgccagag aacatcaaga aaactactgg ctcagacagc cgctatacta cgaaacttac
     4441 ttctctatga gaagagcaca ggccaatgtg attaaagaca tgctggaaaa tctagagaga
     4501 atccagcaac cagcttatta tggaaaacat atctatggtt tattgattta tactgcagaa
     4561 acattttcag agtcaaatga tggcagacaa attttaacac gtattgaaga agtttatgta
     4621 ttgtatcggc aaatgcctct gccgactagt cgccctgaat tcgaggatcg agcagaattg
     4681 ttccagtttt tgcagtcttt taaaagtttt attgaaatca aggcagaatt ctcccaacaa
     4741 aaaatccaaa aatagaggtt gtgaattcag ctaatattca ggtcaaaaag ctgatttcac
     4801 aacctcttct ttaaacggtg ttctcctgtc aactcctcgg tcacaagtca caatattcaa
     4861 caaacatgag aagctgtttt ttgaaattgc tccttgtgac tcggagctga acggtaggct
     4921 cacaacctct ttcttaaacg gtgttcaaaa gctttcctct gcggtaataa gtccagccaa
     4981 acgaaaaata ggaggagcta tttttgtttg gctgttctta ttacttgatg cagaacagct
     5041 ttttcacaac cttttaaaat tacatatttt tccatagaac ttgcatttga tccaagattt
     5101 cgatgacatc ttttgtcaag aagagagagc gattgggcaa gtcaccttca atcatgtgta
     5161 ccatattctt tatttcgtaa ttcattgctt cagatgtatt cccgctttcg atgaattcct
     5221 ttgtaccatc tgtaaataag atctccgcac gatcagcacg cgggtaatca gcaatcgtca
     5281 cataaccgtt ctcaaaggca acgataccga cttttggcat ttttgcctga aaagtcaagc
     5341 tgacagcagc aagttcattt tctttgttac gcaagatagt gacggattgt tcgtccacgc
     5401 ctgtttcaaa aggaaccatt gtagaggcaa ctacttcagg ttgagagctc aaaaagaaac
     5461 gagcgaaaga tacagcatac gtaccgatat ccaacagagc tcctccagct aattctggat
     5521 tgaagaaccg gtttttaggg tctggttctt tgtaactgcc gaaaggtgct tggatcattt
     5581 ttaaagcccc cagttttcct gtatccatta ttgagcgtaa ctgctgataa agtggcatgt
     5641 tgaagattgt catcgcttct gctaagatca aattcttttc ttctgctaag cgcatagcgt
     5701 cagccagttc ttttttattc atcgtgatgg ctttttcaca tagaacgtgt ttgcctgctt
     5761 ccaatgctgc caagatatgg tcgatatgct gtctatttgg tacagcaatg tacacgatgt
     5821 cgatttcagc atcgtctaac atttcttgat agcttccgta agcttttgga atattgtaac
     5881 ggtgagcgaa gttttccgct ttttctgaag ttctggaagc aactgctgtt agttcgctcg
     5941 tctcttgatt gaagctttcg gcaaactcat gtgcgatggt accaagacca acgattcccc
     6001 attgatattt tttcatctga aaacgctcct tcatagttgg attgagtttc ttgtctgtct
     6061 attattataa caaaaatcac gaagaataaa gaggagagga cttgaaacat taaaaaaaat
     6121 gaaagcagcg atttaagagg tgctttttga tcaaaactta agtagaatag ttaaaaggag
     6181 gggataaacg tgcatcaagt atatattgat cttattatga gtgtattatt agaacagttt
     6241 gaaactgaaa aaaatttttt agatgaatac ttgctgatca gtccagaaca atggcaaaaa
     6301 tggaaagatg gcgagaaaaa tctgccatct gaagcgatgc agaaagtaaa aaatcttttc
     6361 actgattatg aatggatgct aacgcaaaag attttgcgtc aaacgattat ttttcccgaa
     6421 aaacgaaacg ttgccgttgc agagtacaag caaatcaaaa cacgcatcgc gcaaaaatgg
     6481 cttagcagca aagcagcaaa agtcgagctg atccctttga aaaataatca gcaggctggt
     6541 agataccttg acttgaaagt aagtattcaa tatgatgaat ggggctatga tgatattttg
     6601 aattttcgtc tgccagcaga tgttcaaaaa cagatcgaag gagaaaaaat cgaacttctc
     6661 gattgggtga atgaaaatct ggaagaaact tacgttcatg aggcgaatgg ggaacgtgaa
     6721 gaacaacgaa aggatttttc aagtgaataa atttttagga tttggagcag ttgtgattat
     6781 gatcgcagca atgggctatg ttatttttag cgatgatgat tcgcatgttc aagccggcaa
     6841 tcaagcagtc gaacgaacaa gcaccaaaac aactgctagt aaagaaaaga acagtcaaac
     6901 aaccagcagc aaggtaaagg acaaagaaac aaaagatcgt acgtccaaac aattgccaga
     6961 tgttaaaaca gacgattgga agctagtttt ggttggtcca gatcacaaat tagaaaaaga
     7021 aattgatgag tctactcaat tggcatcatt aagtaacgga tacttgatcg acaaacgcat
     7081 tgctccagaa tatgaagcat ttgccaaggc tgctgaagca gctggatttc cattagtgat
     7141 ggtctcagct taccggtctg tatcatccca acaacaagtt ttcgaacaaa atgtccaaga
     7201 tgtgatgagt cgtcaaggtg tatcagaaga ggaggcgaca aagattacta aagaaacgat
     7261 tacggagcca ggatacagcg aacaccacac aggtctggca gtcgatgtcg tcgatcaaaa
     7321 ctggtacaac agttatcctt ctactgtatt ggatgcaagt tacggagatc agcctggagc
     7381 taaatggatc gcagataatg ctgcgcagta tggatttatc gttcgttatc caaaaggaca
     7441 cgaagatata actaagatca catatgaacc ttggcactta cgttatgtcg gtaaagaaaa
     7501 cgctgagtat atcaccaaac accaattgac gctagaagaa tatctgaatg aattaaatga
     7561 aaagtaggaa ttgatcatgg cgaaaaaaaa gtggaaaaag caaaagcgca agaatatccg
     7621 ttggccagca gtggttgccg gtgtagcagt gatactctta gcctttgttt tttccttgaa
     7681 gaatctgtct tctcctttta ctgataatca gcaagaaaat gaagcgcaat cccatcagca
     7741 atttattgaa cggcttgttc cgcatgccca agaactacaa gatggttatg gagtgcttcc
     7801 tagtatcatt ttaggacaag ctatattaga atcaaattgg ggaaaaagta ccttggctag
     7861 caaatacaat aatcttttcg ggatcaaggc ttatggggat cagaagaaag taagcctgga
     7921 aacaaaggaa tttgttaacg aagaatggat aactatccaa ggagacttca aagtgtatga
     7981 ttcatgggaa cagtccatgg atgaccatac acaactgttc gtccaaggag tagattggaa
     8041 tcctgcgctg tatgaaaaag tgattacggc caccaactat caggaagccg cacaagcatt
     8101 gcaagatgca ggttatgcga cagaccccgg ttatgcgcaa aaaatcatcc aggtcatcga
     8161 gacgtatcaa ttgaatcagt atgaccggag atagcaaagg agaagtggct gatggcagaa
     8221 caatttacgc cagaaatcat gacggaattg agaaaagata tcgtaagagt accggaaatc
     8281 attttagaag caagcgggat caagatattt ggaaaattga tccgttcgat catctttact
     8341 acggatattg cgatcatacg taatacaaat gcgaatgcag taattgctgt gtatcctttt
     8401 acacctcatc cagcgattac taaaagcatt attgaggcag ctgacattcc agttttctcc
     8461 ggtgtaggtg gtggtttgac acaaggtgct cgttcttcct acatgagcat gttcgcagaa
     8521 gcgcaaggtt cgttaggggt cgttttgaac ggtcctacgc ctttagagac agtaaaagaa
     8581 gtttgtcgag tagttgatat tccagttatc agtacggtaa cgtcgaaata tacagaaata
     8641 gatgaaaagt tagcagctgg tatttcgatt atcaatatca gtgcaggtaa agaaactgca
     8701 gaagcagtac gccattttcg taaaaaatat cctgaacttc ctatcattgc gacaggtggt
     8761 ccaacggaag aaagtattgc tgagaccatt caagcaggag caaatgccat cacatatacg
     8821 ccaccaagta gcggagagct ttttagtcgt aaaatggaaa aatatcggga aactgagaaa
     8881 aagaatcatg aataacaggt gagaatcaaa agacgaggaa gagcagatag tggctcttcc
     8941 tcgtcttttg tactccagta ctatttgttc agaattcaaa aaaaagagaa atttttttgt
     9001 ttttttacct gtaatgtaca taagaagagt taaaaccttt tgttattatt agcattctaa
     9061 caaaaaaatg tcttgattta ttgaagcgct ttctatagaa tgaaaaacga ataaaaacta
     9121 aaaaaggcac ccgacaattt agtagcagct aaattgtcgg ggcttgaaaa gtcagcggga
     9181 actgaccaat caagttgcca tagtcaatgc ttgtgcatcg acgtgtatat tgtacctaat
     9241 ttcatacaga attactagtt atttttctgt tttttaggga agagcgcaca cgaagaagca
     9301 tgcttattta gctatgtcaa ggatattggg cagttgtctg atatcctttt ttctttgcgt
     9361 aattatcctc ccatcattga agcaagaagt gtttttagcg atgaaagaag aatcagcact
     9421 atctaatttt tcttgaaagt attgagttgt tcatcctagg cagaacgata aggcaagtct
     9481 tggtttttat tccccttgaa aaagatcgtt taataaatat gttctgtata aaaatacagt
     9541 atattgaaaa ataacaatgg aagtagaggt gcagagaatg aagaaaagaa tgagaatcat
     9601 cttagcttta agtgttatgt tattcttctt gacggcttgt gggcaagaag atgacaaaaa
     9661 aatcacgctg aacatggctt ggtggggttc gcaagtccga cacgatgcaa cagtaaaagt
     9721 aatcgagctt tatgaagaaa aaaatcccca tgtgacgatc gattatgaat tttatgatta
     9781 tgaaggatat tacacaaaat taggtacact agcagcatca gatgatatct gggacatttt
     9841 ccaactgaac aatcaatttc ctcaatacat caatcagatt gaaccattag atgaatatgt
     9901 ggagcaaggg ataattgata tttctaatgc agatgaatcc tatttagcaa cgacgactta
     9961 cgacgggcaa ttagttggac tgtctaacgg ggtgaattcg tttgcgattg cttacaataa
    10021 aaatatattt gaacaattaa atatccctga accagcttca aattggacat gggatgaatt
    10081 tgaacaaatc agctatcaaa tcactgaaga attaggaatc tatgctattt caagattcga
    10141 agattttata gctggctgta ttattcaaat accacaagtt gagaaaggat tgaattttta
    10201 taatcgggaa gatctatcta acagcttggg atttgaaaat ccagactatc taacagattt
    10261 tttccagctg cgtaaaaatc tggtagacaa aggggcccat ccagaaccag gtggtggagc
    10321 tgcaagtacg acggatctgt cttttcaggc ggttagggat agagaagcgg caatggtgtt
    10381 tctatcaagt aaccagttga cagaaatatt gtcaggggct cctgaagata tggagattca
    10441 gttgattaat ccacctcgca gaaaagcaga tggagaatct ggtatccctt tacgttcttc
    10501 gcaaatgctg agtatggcaa aaagctcagc ccataaagaa gaagctgcta agtttattga
    10561 tttctttcaa aacagtgaag aagccaatga aattttaaaa ggagaacgtg gtgtaccaat
    10621 catgtcgaat attcgagaac agctcgctcg tgcagatgat gcaactttgc aagcgacatt
    10681 tgattattta gaaatgattt ccgatatgga caacggcgaa gtgaatgtct ttgatagtgc
    10741 tttgaatccg gaaattcaag accaatacaa tcttttagtt gaaaaagtca tttatgaaga
    10801 agtaacagca gaaggaaggc atgaaaaaag aaaaactaaa acggttcttg gatcaagaca
    10861 atgtggtcgg ttacgtattt gcttctccat tcatcctagg atttctattg ataacagtga
    10921 taccgatgat cttgtcactt tattattcgt tcaccgatta tcgtttggga aatccagtct
    10981 catggatcgg cttggataat tatataaaga tgctgagtga tgcccgtttt ttgaactcca
    11041 tccgagtcac ttttctctat gtatttgcat ctgtacctgt gaaactgatc tttggtctat
    11101 tgattgctta tcttttaacg aggaatatca aaggaacgac tttctttcgt tctttgtatt
    11161 atgtgcctag tttgatcggt ggcagtattt ctatcgctct agtttggcgg gagttgtttt
    11221 ctaaaaacgg gttagtgaat attttcctgg aacgattagg cgcacctgcg ctttcctggt
    11281 ttagcgatca aaaactggtc atgatccctc ttgttttgat gtctgcttgg caattcggtt
    11341 cggcaatgat catctttgct gcaggtatca aagagatccc aggctcgtat tatgaagcgg
    11401 cagagatcga tggagcgaac aaatggcaga cgttttacaa gatcacgtta cctcttcttt
    11461 cgccgatcat tttgtataac ttggttatgc agacaatcgc ggcgtttatg acttttacgc
    11521 aggcatttat cattacccaa ggtggtccga atgatgccac gaacttttat gctctttatg
    11581 tgtataacca agctttttct tttaattcta tggggtatgc tagtgctatt tcgtggatca
    11641 tgctgttgat tatgggagct gtcacctttc tgattttcaa atcatcaaaa aaatgggtcg
    11701 ttgtgtgaat gagaggagga agaaatgaat aatacagtaa cgaaatggtt tcaatacggt
    11761 tttattttgc tattaggtct agtcatgatt tatccggtct tatggatgat cgcaggttct
    11821 tttaaaacgg atcaagagat tatgagtggt tcgctgaatt tgattccaga gacttttcaa
    11881 tgggaaaatt acgcaaaagg atgggcgggg tttgctggaa tttcattttt tactttcttt
    11941 aggaattcgt ttgtcatctc tgcagtttcg acgataggga cagttctgag ctcgacttgt
    12001 attgcttacg cattatcgag aatcgatttc aaaggaaaaa gattctggtt cgtcgtcatg
    12061 ttagtaacga tgatgattcc tgcacaagtg attttgattc cgcagtttat tatttacaat
    12121 cggctgaatt tggttgggac gtatgtgccg ctgattttac cacatttttt tggacaagca
    12181 tttttcattt accagatcat gcaattcatg gtcagcatcc ccaaagagtt agatgaatca
    12241 gcgattattg acggatgcag caagtatagt gtctttacca aaattatttt tcctttgctg
    12301 aaaccttcga tcatcacgac gatcatcatc cagttttatt ggaaatggga cgacttcatg
    12361 ggaccattga tttatttaaa caagccgaga tcttacacgg tatcgattgc catcaaatta
    12421 tttgcagacg ctggatctag tacgacttac gcgagtatgt ttgcgatgtc tacgttgtcg
    12481 ctgctaccgg tttttctgat tttcttattc ttcaataaat atcttgtcca ggggatcagt
    12541 acaagcggtc tgaagggctg aatatgaaaa agccaataga agagatgttt ctttacaagc
    12601 tcaactacta tgtctgtcgg atttttttaa ccagtcttat cagtaatgct cttttcgctt
    12661 tagttctagg atgtttgttc actttaggat tagtgctgtc tgctcgtgtt gtctatctga
    12721 tttatttttt gatgatgtat ccttcttatt atctatctgt ccattacttg gcagaaaaaa
    12781 aggtatgttg gaggaaaggg aaaatcaaac ggatgtgttt catcattacg ttgtttcagc
    12841 tgtttgtcat tatttttgca gttgattggt atttattagt gggaaagatt ggagcttggt
    12901 atctgattcc ttttctattg tgtctgttgc tttttgtatc ccatagtttt tttagtacgc
    12961 taattgatat ctgcaaagaa gataaagcag atagtcttgt ttatttgatc aaaaggaata
    13021 tctggctgtt gtcaaaaaac gtcaaggggt ttctgtggta tttatgcctt ttgtatggac
    13081 tgttctggtg tatacagcct gattttttac tcactgcatt actgtatcca gggctgccat
    13141 ttgtcacagt attttgctta aaagaaaagg agaatgactt taaatgaaga aaaaatatgt
    13201 acaagtcgga cttggtggga gagcgcgttt tttttatgag gcaattgcct ctacgtatca
    13261 tcaaacttgt gaactagtcg gtttttgtga tgtatctcaa acaaggatgg attatgcaaa
    13321 tcggatactt gtgaacaagt atggacagaa aaaagtaaag acatgtatgc catcacaatt
    13381 tgagcaacta ctggatgacg tacggccggc tgaagtcatt attacgtcag tagatcgaac
    13441 gcatcatgat tatatcattc gtgcaatgaa aaaaggatat gatgttattt gcgaaaaacc
    13501 tctgactatc gatgaggaaa aagctcaaga aataataacg gtccaaaaac agacgggaag
    13561 aagcctgcga gtcactttta attatcgtta tgcaccgtat cacacgaaga taaaggaact
    13621 gatagaaagt gatgtgattg gtgatgtgtt ttctatccac ttcgaatggc tgttaaatac
    13681 gcaacacggg gcagattatt tcagaagatg gcatcgaaat aagcacaata gcggcgggct
    13741 tcttgttcat aaatctaccc atcattttga tctagtgaat ttttggttga acagccgtcc
    13801 ccaaaaggta tttgcattcg gtggattaaa cttttatggg atgcataatg cggagaaacg
    13861 cggagtagaa atattttaca gtcgatgcta tggaagcgaa ctagctaaag ctgatccatt
    13921 tgctatagac ctgtcagcca atgaggaatt gcgagaaatg tatttagaag cagaaaaaga
    13981 cagtggatat attcgtgacc gcagtgtatt ttccgatgat atctctattg aagatacact
    14041 aggcgtaacg gtcaaatatg aaactggtgc gattttgacg tattcgctaa acgcgtatat
    14101 gccgtgggaa ggattcaatg tttctttcaa tggcagcaaa ggacggatcg aaatgaaagt
    14161 gatcgaaaag tcttacatca atgcaggtgg agataaaaag gatgaaggag cagccgttct
    14221 tcagtccatc catgtctatc cgatgttcgg tgagccatat gaggtaatcg tcgaacaaga
    14281 agcgggcgga catggcggag gagatccgcg tttattagat gatctatttg gtgaagaaaa
    14341 agaagaagat ccgtggaatc gggcagcaac tcacgtagat ggtattttgt cgattttgac
    14401 aggaatcgca gcaaatcatt ctattgcttc aggaaaagca gtagtgattg acgagcttgt
    14461 cagcttaaaa gaataagaat agatttacgg aaattacttc ccgtagttta taaaatcttg
    14521 ccttattgcc gataggctgt cttttgaaca ctatttattc ggaaaaaggg gctatgacat
    14581 gctttttgtc atgccttctt tttatgtggg ctgctttttt agataggaga aaataaaaat
    14641 aactatttaa acccgaacaa aaaggaataa tgcattaaaa atcattagat tttacaaaaa
    14701 aataagtaaa ccacgttgtc agtttcagaa agtagtgtta gactgttttc aatatttgaa
    14761 acaacatttt ttattcaaat atggcaaact gccttcacgt gcaaaacagt tgcaagaaaa
    14821 tgaatttaca ctattattag ttttacatac ctacttatat atcgtcataa tatggatgac
    14881 agtttctagc cagtaccgta aatgctggac tataagtaaa agattggcta tttcaccctt
    14941 cttttcttat gataaagtgg agggtgtttt tctttaacag aaacatagta ggtttttgca
    15001 caaaagcggt tatttcggta ttaataaatg ataaaggagc aaataaagtg aaagaattag
    15061 ttgaacgtat tcaaaaagac ggacgtatat taggcgaggg cgtattaaaa gtcgacagct
    15121 tcgtcactca ccaagtagac cctgtattga tggaacaaat gggaaaacgg tttgcagaag
    15181 tatttaaaga acaaggaatc acgaaagttg tgaccattga agcttctggg atcgcaccag
    15241 ctttatttgc tgcaagagaa ttagatgttc caatgatctt cgctagaaaa gcaaaaagtt
    15301 tgacgatgga tgaagaatta ttgacagctt ctgtttattc ttttacgaaa caagtgacaa
    15361 gtacgatttc tatttctcgt aaatttttat cttcagaaga caaagtattg attatcgacg
    15421 atttcttggc aaacggacaa gcggcaaaag gattgatcga attatgccag caagctggag
    15481 cgcaagtaga aggaatcggg atcgtcatcg aaaagtcttt ccaagatgga cgtcagttac
    15541 ttgaagaaat gggattgaga gtcgtttctc ttgcgcgtat tgcttccttg acgaatggac
    15601 aagttgagtt tcttgaggag gacgcataat cgtggaaaaa gaaacacaaa atggaaaagc
    15661 tgcggtttta gggctgcagc atctgctagc gatgtatgca ggagcagtag cggtgcctct
    15721 tttgatcggt acaggactag gctttaatca agaacagatg acttatttga tttcaattga
    15781 tatctttatg tgtggattag cgacattatt gcaattgaca gttaaccgat ttttcggaat
    15841 tggcttgcct gtcgttttag gatgtgcgat ccaagcagta gctccactga tcttgatcgg
    15901 aaccaatgaa ggagtaggag ctatctacgg atctattatc gcttctggga tctttgttgt
    15961 gttagtttct ggtgtctttt ctaaaattaa aaaactattc cctcctatag taacaggaac
    16021 ggttatcacc gttattggtt tgactttgat tcctgtagca atcgagaaaa tgggtggagg
    16081 taatgcaaca gcagctgatt ttggtgacaa aacaaatctg ctacttgcat ttgtgacgat
    16141 tctcctaatt gtaggcgtgc aaatgctggc aaaagggttc attcgttcaa tcgcggtatt
    16201 gattggttta gtcggaggaa gcatcttagc tgctttcatg ggaatgattg atctgagcgc
    16261 gattggagat gcgccgttgt tccatgttcc acaacccttt tattttggta aaccaacttt
    16321 cgacgtatgg tcgattttac taatgatcat catttccatg gtcagcatgg tagaatcaac
    16381 aggtgtatat tttgcacttg gtgacattac tgggaaaaat gttggagaag aagaattgaa
    16441 aaagggatat cgtgcagaag gacttgccgt tatcttaggc ggtattttca atacattccc
    16501 ttatactggc ttttctcaaa atgtcggatt ggttcaactt tcgggaatca aaacaagaaa
    16561 acccatctat ttctctgcat tcttcttgat tgttttagga ttattcccta aaatcggtgc
    16621 gttagcacaa atcatccctg agccggtact tggtggagga atgttagtca tgttcgggat
    16681 ggtggcagtc caaggaatcc gtatgctgtt ggaagttgat ttcagcaatg ataaaaatct
    16741 attgattgtt gctgtttctg ttggatttgg gctgggtttc aatgttacgc cgacattatt
    16801 cagtcaaatg ccgcaaacat tgcaaatgtt cactggaaat ggcatcgtaa tgagtagcat
    16861 tacggctatc atattgaatt tgatctttaa tggattgaaa aaagatgaag aaatattgga
    16921 agaagccttc aagaagtaat agagcaaaag aaaggttgtg aaatcagcgt tttgacctga
    16981 ttgttagaga aacaaacgga aaaatagttc ctcgtatttt gccgtttgtt tgagctaata
    17041 ccgagggtca gctgattgaa caccgtttat ccataaaaag gaatgtgacc taacttttgt
    17101 cacattcctt tttttcatct ttaataaaca cgaacaataa aatgtttttc tttgttaaca
    17161 ttcgaaattt ctttgacatc aaaaaaagtg attgttaaaa tatgaatgta gaaaaactgg
    17221 gaggaaaaac aatgacagca aaaatctctg tgattatggg gagtacttca gattgggaaa
    17281 caatgaaaga agcatgtcaa atattggatg aattcggtgt gtcttatgaa aaaaaggtcg
    17341 tttctgctca tcgaacccca gatttgatgt ttgaatatgc acaaaacgct cgaaaaaaag
    17401 gaatcaaaat cattattgca ggagcagggg gagccgcgca tctgccgggt atggtcgcat
    17461 caaaaacaac tttgccggtt atcggtgtac cggtccaatc acgaacatta aatggtttgg
    17521 attcgcttct ttcgattgtc caaatgcctg gaggagtacc tgtagctact acagcaatcg
    17581 gaaaagcggg agcaattaat gctggactac ttgcgataca gatgctttcc atgtatgatt
    17641 tagaattgga ggctaaattg gctgaaagac gttcaatgtt ggctaaaact gttatagaaa
    17701 gtagtgatca acttggttaa acctttgttg cctggttcaa cgatcgggat cgtcggtggc
    17761 ggtcaattag gtcgtatgat ggcgattagt gcaaaagaaa tgggctttaa agtaggtgtg
    17821 ctggatcctg tgagcgattg tccagccgca caagttgcag attggcacat tgaaagtgtc
    17881 tatgatgaca cctttgcgct gaaagagctt gccagaagaa cagatgtcat tacctatgaa
    17941 ttcgaaaacg tcagtgtaga ggcgttaaat gcgattttac ccatgtcatt tattcctcaa
    18001 gggacagact tgcttgcgat tacgcaagac cgcctgttag aaaaatcttt tttggaaacg
    18061 aataacatcg tgattgctcc ttatgcaacg atcgtcagtc cgacggatat ccaagatgcg
    18121 attgacggga tcggctatcc ttgtgttttg aaaaccactc gaggcggcta tgatggcaag
    18181 gggcagtatg ttttgaaaag tcgggcggat ctagctcctg ccatggacct tttaagagaa
    18241 ggaacctgtg ttttagaagc atggatcccg tttgaaaaag agatttcgat catggtggca
    18301 ggaaatggtc aaggagattt cacgactttt ccggtcgtgg aaaacatcca ccacaacaat
    18361 attcttcatg aaacgattgc tccagccgcg attgatcagg atgtgatcga ggaggctgag
    18421 cgtattgccc gtgtgattgc agaagcagtg agcctttcag gtgttttagg ggtagaaatg
    18481 tttttaacaa aaacaggcgg gctatatgtg aatgaattag ctcctcgtcc tcacaattct
    18541 ggacattatt ctattgaagc ttgttctatg agtcagttcg atgcccatat tcgaggtatt
    18601 tgtggttggc cattaggaga aaaagtacgt ttgataagca atgcagtaat ggtcaatgtt
    18661 ttgggagaac aactgctgga aagctatcgc ttgattgaga ataagccaga ctggcaattc
    18721 catttctatg gaaaaaaaca agcaaaaaaa ggtcgcaaga tgggacatat cactatcttg
    18781 acagaagaca tccagcaaac gctggaagaa atcaaacaaa cgaatatttg ggattagagg
    18841 agaaaaaaca tgttagaacg ttatacacgc cctgaaatgg ggaatatctg gacggaagag
    18901 aacaaatacc gtgcgtggct cgaagtcgag attttagcag atgaagcttg ggcagaactt
    18961 ggtgaaatcc cgcaagaaga tgtgaaaaaa attcgtgagc atgcatcatt tgatatcgat
    19021 cggatcttgg aaatcgaaaa attaacacgc catgatgtcg ttgcgtttac acgagcggtt
    19081 tctgaaacat tgggagaaga aagaaaatgg gtccattatg gtctaacaag tacggatgtc
    19141 gtagatactg cttatggcta tctcatcaag caagcaaatg atattttacg ggaagacttg
    19201 aaacgtttta cggaaatcat tggcgaaaaa gcaaaagaac acaaatatac agtgatgatg
    19261 ggtcggacac acggtgtcca tgctgaacca actacttttg gattgaaact tgccacatgg
    19321 tattcagaaa tgaaacggaa catcgaacga tttgaacatg cggcaaaagg agtagaagca
    19381 ggaaaaatct caggagcagt gggaacgttc gcaaatatcc cgccatttat tgaagagtat
    19441 gtatgtaaac aattggggat ccgtccacaa gaaatctcta ctcaagttct ccctcgggat
    19501 ctacatgctg aatatttctc agcgatggcg cttattgcta caagtattga acgttttgca
    19561 acagagatac gcggattgca aaaatccgaa acacgtgaag tagaagaatt ttttgcgaaa
    19621 ggtcaaaaag gttcttctgc tatgccgcac aaaaggaatc cgatcggttc agaaaatatg
    19681 gctggtctag ccagagtggt gcgcggacac atggtcactg cctatgaaaa tgtcacatta
    19741 tggcatgaaa gagatatttc tcattcttca gcagaacgaa tcatcttgcc tgataccacg
    19801 attttattga attacatgtt aaatcgtttt ggcaatatcg ttaaaaatct aacggtcttt
    19861 cctgaaaaca tgaagcggaa tatgtctgca acatttggtt tgatttatag ccagcgcgtt
    19921 atgctgaaat tgatcgataa gggaatgaca agagaagaag cttatgatct tgttcagcca
    19981 aaaactgcgt acgcttggga caaccaagtg cagttccgcc cgctgttaga agaagatcca
    20041 accatcacat cggtattatc taaagaagaa atagacgatg cctttgatta tcatcatcat
    20101 ttgaaaaacg ttgacgtgat attcaaacga gtaggtttgg aataaagaag agtgagttgc
    20161 aaaattcaat taaaatagaa tagcaaaaat aaccaaaaaa gaagcagatc cgttagcggt
    20221 tctgcttctt ttagtttatt atattttctt atcaataata ggaacctgat tttaaaagcg
    20281 gctacctctt taccgtgcct ctttacatac aaaaaataga ggaattaagc gtaaatggct
    20341 tggctattat aactaaaatg ctggttatgg agatgttgca gcaagttttc gagaaaagcg
    20401 tattccatct cttgtccatt gaataagatc tggatacgat aatgattttt gaaacgcttc
    20461 tgttcatgaa tctgactgat tgaaagttcg cctttgtgtt ctgtctgata aagaaaagtc
    20521 cttttacggc attcagcaca gcgcttggtt gctcggcgga tttttcggaa tgtataaaaa
    20581 ctagtatctt tattgcataa ataaacgtct ttgatcgaca gcaaatcatt atatagacag
    20641 gaacgttctt cagctgttaa ttctgataaa aaaagctgac cttgttttat tgcatcttcc
    20701 atcggcaagt gcctcctcga acccattatc caaattttta gttaattcta atataccatc
    20761 attaagtata aatgtaaacg cttgttgatg gtataagatt ttcgtttctg ataaaacacg
    20821 aacattaaaa gaatctaatg ccttaaatgt acatattttt gttgatttat aaaaagatga
    20881 ttgctaaaat ttaaagtgaa aaaacgaaca aaaaacgatg tatttgcttt taatgtaaat
    20941 aaaaaggaga gacgtacatg gagaagaaag aacttttgta tgaaggaaaa gccaaaaaaa
    21001 tatatcttac ttctgatgag aacttgattt ggatagaata tttagatcaa gcaactgcgt
    21061 tgaatggcgc gaggaaagac cagataagtg gaaaaggaga attgaataat caaatcacaa
    21121 gtgcaatttt ccgttatctg actcaaagag gaataaaaaa tcattttatt gaacaattgt
    21181 ctaaaaccga acaattagca aaaaaagtac aaattatccc tctggaagtg gttatccgaa
    21241 atattgcagc aggaagtttc tcaaaacgtc taggtgttcc cgaaggtaaa gaattcatca
    21301 aaccaatcat tgaattctac tataaggatg accagttgga tgatccattt atcaatgaag
    21361 agcatatcta tttcttacaa cttgcaaaac cggaagaaat cagtgagctg aaagcccaag
    21421 cgttggctat taatgaaatt ttgaccgaat tatttgctaa ggtcggtttg gttttagtag
    21481 atttcaagct agaatttggc cggtcgaatg gacaaatttt gcttgcggat gaagtaacac
    21541 cggatacttg ccgattatgg gatgaacgta cgaatgaaca tatggacaaa gacgtttacc
    21601 gcagagatct aggtgatttg atcccagtct atcaagaagt atataaccgt ctatcggaac
    21661 taactgaaga aaaggagcga gtatgatgta tttcgtaaaa gtatatgtaa cctataaaga
    21721 ttcagtgtta gatcctcaag gggaggcagt aaaaaaagca gtccatcgta tgggatatga
    21781 aacaatcgaa gatatccgaa taggaaaata ctttgagata caacttgctg atgcagaaga
    21841 accaatcgat acagtgattg aaacgatttg tgacaagctg cttgcaaatg taaatatgga
    21901 aacataccga tatgaaatca aaaaaatgga ggaagtataa catgaaattt gcggtcatcg
    21961 tttttccagg ttcaaattgt gacgcagata tgttatgggc agttcgcgag atcatgggcg
    22021 ctgatgccga atttgtacgg catgatgagt cctctttaga aggatttgat ggggttttac
    22081 tgccgggagg attctcctat ggtgactatt tgagatgcgg tgcgattgct cggttttcta
    22141 caatcatgga agaagtgatt cgttttgctg aagaagggaa accagtattc ggtacatgca
    22201 atggattcca gatattgaca gaagcaggac ttttgccagg tgtactgaga cgaaatgagt
    22261 ctttgcattt tatttgcaaa actgtaccat tgaaagtcaa ccccaagacg gcctttactt
    22321 cagagtatga agccgacgaa ataatctatc ttccagttgc tcatggggaa ggaaattatt
    22381 tctgtgacga agaaacactt caagaactaa aaaaaaataa tcagattgcc tttacttata
    22441 atgacaacat caacggcagc gtggaaaaca ttgcagggat tacgaacaaa gcagggaatg
    22501 tgttaggaat gatgcctcat ccagaacgtg cagtagaaga cttgctagga tcagaagatg
    22561 ggaaacggtt ctttgcttcc attttaaaaa acttcggaaa ggtgaccgta tagatgaaaa
    22621 cattgaaaga accgacagct gaagagatca agaaaaaaag aatctatgcc gaatggggat
    22681 tgactgatga agaatatcga atgatcgaag aagatatctt aggaagaatg cctaattata
    22741 cggaaactgg actgttttct gttatgtgga gcgaacattg ttcctataaa aattctaaac
    22801 cggttctacg caaatttcct actagtggac ctcatgtgtt gcaaggccct ggagaaggcg
    22861 caggcatcgt cgacatcggt gatggactag cagttgtctt caaagcagaa agtcacaatc
    22921 atccttcagc cgtggagcct tatgaaggag ctgcgacagg agttggcgga atcatcagag
    22981 atattttcag catgggggct cgtccaattg cgttgctaga ttcattgagg ttcggcgaat
    23041 tagataatcc aagaacgaaa tatttactag aagaagtagt tgcaggtatc ggcggatacg
    23101 gcaattgtat cggcataccg actgtaggcg gcgaaattgc atttgatcct tgttatgaag
    23161 gaaacccatt agtcaatgca atgtgcgtag gtttgattga acacaaacat atccagaaag
    23221 ggcaagccgc tggcgtcggt aattcgatca tgtatgtagg tgcaaaaaca ggtcgcgacg
    23281 gtatccacgg agcaactttt gcctcagaag aatttaatca agaagaagaa cagcaacgtt
    23341 ctgctgtaca agtaggcgat ccttttatgg aaaagctgct gcttgaagcg tgtttggaat
    23401 taatcttgca tcattcggat tctctaatag gtatacagga tatgggagca gcaggacttg
    23461 tttcatctag tgcggaaatg gcttcaaaag ctggctcagg acttatattg acattagatg
    23521 aagtcccaca aagagaaact gggatgacac cttatgaaat gatgttatcg gaatcacaag
    23581 aacggatgtt gatttgtgtt aaatcaggag aggaagaaaa aatccaagaa ctatttcaga
    23641 agtatgatct agatgcagtc actatcggta aagtaacaga cgacgggcaa tatcggctat
    23701 atcatcaagg agaagaagta gcaaatctgc cagtcgatgc attagctgaa gatgcaccag
    23761 tttatcataa agaaatgaaa gaaccagctc gaatcgtaga atttcagcag ttagctcctt
    23821 atcagccagt tatcgaagaa cctggacaag tattgcttga tctgctgcag caaccgacga
    23881 ttgcttcaaa acgttcaatc tacgaaactt atgattcaca agtacaaacc aatacagttg
    23941 tccgtccagg aagcgatgct gctgtgatga gggttagagg gacgaataaa gcgttagcta
    24001 tgaccacaga ctgcaacgcg cgctatctgt atttggatcc aaaaatcgga ggacaaatag
    24061 cagtggcaga agctgctaga aatattattg ctagcggtgg taagcccttg gcgatcaccg
    24121 actgcctgaa ctatggttct cctgataaac cagaagtttt ttgggaatta tcgacttcag
    24181 ctgacggaat tgctgctgcg tgtgaaacgt taggaactcc tgttatttca ggaaatgtct
    24241 ctttatataa tgaaacggat ggtcaagcaa tctatccaac accaatgatt ggtatggtcg
    24301 gattgattga ggatcataaa catatcacta ctcaggaatt caaaaaatca ggggatctaa
    24361 tctatatatt aggtaagacg tttgcagatt ttgatggcag tgaattacaa aagatgcagc
    24421 ttggaagaat cgaaggtgtg atccgaaact ttgatctatc cattgaaaag agaaaccaag
    24481 agttagtatt gacagccatc caaaacggat tgattgaaag tgctcatgac tgttcagaag
    24541 gtggcttggc aattgcactg gcagaatcag ctttcaaaca tcagctaggg atatctgtac
    24601 agtttgaatt gtcctctgct caattatttg cagaaactca atcaagattt gtgttgacag
    24661 tcgcaccgga aaataagaca cgattcgaag aaatgatggg agatgctgct gttttagcag
    24721 gtaaggtgac ggatgaagcg ataatcgaaa tttcagcaac tgacggtcag atcaaaatag
    24781 aaactgcagt agcaagaaag tgctgggagg atgcaatcgt atgtcttatg aagtgaagag
    24841 tttaaatgaa gaatgcggga tcttcggtgt ttggggtcat ccagacgccg ctagtgtggc
    24901 atacttcggg ctccatagtc ttcagcatcg aggacaagaa ggtgcaggta tcgtgtccaa
    24961 tgatcgagga aagcttaagg ggcatcgaga tttggggctg ctttctgagg tattcaaaga
    25021 tcaaagaaaa ttgcagcagt taacaggaga agctgcaatc ggacatgtac gttatgcaac
    25081 agcaggcaat ggaagtgtgg acaatattca gccttttcta ttcaaatttc atgatcagtc
    25141 gatcggctta gcgcataatg gtaacttgat caatgctcgt tcccttagaa aagagttaga
    25201 ggaaaatggg gcgatcttcc attctaactc cgatacggag atattgatgc atttgatccg
    25261 acgaagtgaa gaaacagatt ttttaaataa attaaaaagt gctttgaatg aagtcaaagg
    25321 tggctttgcc tatttattaa tgacagaaac ggcaatgatt gccgctttgg acccaaatgg
    25381 ttttcgtcca ttagcaatcg gacaaatgaa aaacggagca tacgtgattg cctcggaaac
    25441 ttgtgcatta gaagttattg gggcacgttt tataagagat gtccagcctg gtgagatagt
    25501 gatcatcaat gacgaaggaa tccagattga ttgctttacg aaggacacgc agctttctat
    25561 ttgttcgatg gaatatatct attttgccag accagattca aacatcgcag ggatcaatgt
    25621 gcatactgca cggaaaaata tgggacggaa tttagcgaaa gaatcgccag taaaagcaga
    25681 tatggtcgtt ggcgtaccta attcttcttt gtcagccgca agcggttatg cggaagctag
    25741 tggaatccct tatgaaatcg gattagtaaa aaatcaatac attgcccgaa catttatcca
    25801 gccaacgcct gagctaagag agcaaggtgt tcgaatgaaa ttatctgctg tacgaggagt
    25861 agtggaaggt aaaaaggtga ttatggtcga tgattcaatt gttcggggta cgacaagcag
    25921 acggatcgtc catcttttga aagaagcagg ggcaaaagaa gtccatgtga ggattgcttc
    25981 tcctcctttg aaatatccat gtttttacgg gattgatatt caaacgcgta aagaactcat
    26041 tgcggcaaat cacacgattg atgaaatcaa agaatgtatc ggtgcagatt ctttaggttt
    26101 tttaagtgaa gagggtctga tcgaatcgat cggtctggaa aaagatgctc cgtattcagg
    26161 attgtgtatg gcttatttca acggagatta tccgacgccg ttatatgact atgaagaaaa
    26221 atatcaggaa tcactaaaag aaaaagtatc gttcttttaa aagcagacca agaagggagt
    26281 atatgaattc aatggcaaat gcatacgcaa aagctggcgt agatgtcgaa gctggatatg
    26341 aagtagtaga acggatcaag aagcatgtca aaaaaacgga acgtttaggt atgatgggcg
    26401 ctataggagg attcggcggt tgttttgatt tatcggtctt tcaattaaaa gagccagtcc
    26461 ttgtctcggg gaccgatggc gtggggacga aattagcagt ggcaatcgaa gcagacaaac
    26521 acgatacaat cgggatcgac tgcgtagcga tgtgtgtcaa tgatatctta gcacaaggcg
    26581 cagagccgtt atatttctta gactacttag caacggggaa aaatgatccg ttacgtttgg
    26641 aaaatgtagt aaaaggtgta gcagatggat gtgttcaagg aaatatggcg ctgattggcg
    26701 gagaaacagc tgaaatgcct ggtatgtact caggtaatga ttatgatcta gccggatttg
    26761 ctgtagggat agctgaaaaa tctcaattat tgacaggaga aacgatcaac ccaggagatg
    26821 tgttgcttgg aatagcatct agcgggattc attcaaatgg gtactctctt gttcgcaaaa
    26881 ttttctttga gcagcagcag ttttctttat catcaaaact gccagaattg aaaaaaacac
    26941 ttggcgaaga attgcttgag ccaacgaaaa tctatgtttc ttccttgctt ccgctgatca
    27001 aagaaaagaa aataaaagga gctgcacata tcactggtgg agggtttgta gaaaatatcc
    27061 cgcgaatgtt accagaatct atggctgcac aaatcaaact tggctcatgg ccgattcttc
    27121 caatattcgg ctgtttacaa aaatatggtg aaattcctga gatggagatg tatgaaatct
    27181 tcaatatggg catcggtatg gtcctggcag tcgaagaatc gaaagtaaaa gaaatagtac
    27241 acgaaattga gcaagctggc gaaaaagttt atgaaatcgg gaaagtgatc gccaaagaaa
    27301 aacatcgtgt ggtgtttata ggagaagaac gatgagaatc gctgtttttg cttctggaaa
    27361 cggaagcaac tttcaagcgc tagccgatta tctatcgaaa aaaggattgg aatcttcgat
    27421 tgactggttg ttttgtgatc agcctgaagc atatgttttg aaacgtgcaa ctgcgttgag
    27481 tgtaccggca gattgttttt cacctaaaga atttgattca aaaaaagagt atgaagaagc
    27541 aattttgcat aaattgaaag aaaaaaagat cgatttgatc gtattagctg gatatatgcg
    27601 aatcattgga ccggtattgc tgaaaaatta tgataaaaga atcatcaata tccatccgtc
    27661 tcttttacct gccttccctg gtttgcatgg catacgtgat gcctttgaag ctggagtaaa
    27721 agaaacgggt gtcacaattc attatatcga ccaaggtgta gatactggac caatcatccg
    27781 gcaggaaaaa gtgagaattg aacaggagga cacgttcgat tctttagaag aaaaaatcca
    27841 tagagtagag catcgaatct atccagaagt gattagtgaa attattgaaa atggaggcta
    27901 tctgaaatga caagagcatt gatcagtgta tcagataaaa ctgggattgt agcattcgcc
    27961 caaggtttga ttgaacaagg agtagaaatc atttcaacag gaggaacaaa gcatgcgttg
    28021 gataaagctg ggatcaaaac gctttcgatc gaagaagtga cagggtttcc tgaaatgatg
    28081 gatggccgtg tcaaaacact tcatcctaaa atccatggtg gtcttttagg aagacgcgat
    28141 ctccctagcc atatggaggc aatggaaacg gaaaatatcc aaccaatcga ctttgtttgt
    28201 gtcaatttat atcctttcaa agaaacgatt atgaaaccag atgtcgaaat agcagaagct
    28261 attgaaaaca ttgatatcgg tggtcctagt atgctccgta gtgcagcaaa aaaccatgaa
    28321 tttgtcacct ctgttgttga tccatctgac tatgaagaag tactttcaga attacgtcag
    28381 tatgggcata cttcactttc tttcagacaa aaactagcgg caaaagtctt tcgtcatact
    28441 gcggcatatg atgcactgat cgctcaatac ttaacggatt gggtggaaga aaaagaaccg
    28501 gaaaaattga cacttacgta tgaacgaaaa caaacgcttc gttacggtga aaatagccat
    28561 caagccgctg ccttttatca gtccgctctt ctggaaaaat attcgatcgc agcagcaaaa
    28621 caactccatg ggaaagaact ttcttataat aatatccgag atgcagatgc tgcgctacgg
    28681 atcatgaagg aatatgacga gccaacggta gtcgccttga aacacatgaa tccttgtggt
    28741 attgggacag catctacgat tttagaagcg tgggaagcag cgtatgaagc agatcctgtt
    28801 tcaatctttg gtgggatcat cgttttgaat cgagaagtag atctgccaac agcagaacag
    28861 atgcataagc tgtttctaga aatcatcatt gccccttctt atacacaaga agctttggaa
    28921 ttactgcaaa aaaagaaaaa catccgatta ctgacagtag actttgcgca ggaagaaaat
    28981 caaaatcgag aagaaacggt ctcagtctta ggtggacttc ttgtacaaga tcaagatctg
    29041 gcaatagaaa atgaagaaga gtggaaagtc gtgacaaaac gtaagccgac tccagcagaa
    29101 gcaaaagcat tagcgtttgc ttggagagct gtcaaacatg tgaaatctaa tgcgatcgta
    29161 ctggcaaacg aacgtcagac agtaggaatc ggagccgggc agatgaatcg agtcggttca
    29221 gtaaaaattg cgattgaaca agcgcaagag aaaatggaag gtgcagtttt agctagcgat
    29281 gcttattttc caatggatga ttcggttgaa tatgcagcca aacatggtat aaaagcaatc
    29341 attcagccag gaggaagcat caaagaccaa gattctattg atatggcgaa taaatacgat
    29401 ctagcaatgg tctttactga tgttcggcat tttagacatt agagaggagt cggataaatg
    29461 aacattttgg tgatcggtgc aggtggtcgt gaacatgcaa taagtaaaaa acttttagcc
    29521 agtccgaagg tggatacggt gtattgtgcg ccaggaaatc cggggatgac aaaagatggt
    29581 atcaattgtg tcgctatcag tgagcgagat cattcagcgt taatccattt tgttaagaaa
    29641 gaggctattg attggagtat cgttggtcct gaagttcctt tgttaaatgg gatcgtcgat
    29701 gattttatgg cggaaggatt gaaaatcttc ggtccgacaa aagcagcagc tctgatcgaa
    29761 ggttcgaaaa gttttgctaa acaattaatg gatacttatc aaatcccgac agctaaatcc
    29821 agaagttttg aatgttacga aaaagcagca gcgtatatca aagaaaatgg tgcgccaatc
    29881 gtagtgaaag cggacggctt agcagctggg aaaggtgtag tcgtggctac cacagtgaag
    29941 gaagcgctgg atgcggcaga acaaatgttg aaccagcatc gatttgggga tagtagcaaa
    30001 aaaatcgtcg tagaagagtt tttggcagga gaagaattct cccttttggc gtttgtccgt
    30061 aataccaagg tgtatccgat ggtcatttcg caagatcata agcgggcatt tgataacgat
    30121 aaagggccaa atacaggcgg aatgggtgct tattcaccag taccgcaaat ttcaaaaaag
    30181 atgattgaaa aagctgtgga agaagtttta aaaccagcgg ctttgggaat gaaagaacta
    30241 ggacgctctt tcacgggtgt tttatatgcg ggtttgattg ctacgaagga aggaccaaaa
    30301 gtgatcgaat tcaatgcgcg tttcggtgat cctgaaacgc aagtcgttct ttctcggttg
    30361 aagagtgact ttgcgcaagt aatcgatgat ttattagaga atcgtacagt agagttaaag
    30421 tggcagcaag aaggatacaa tgtaggcgtt gtggttgcag ctgccggtta ccccgaagaa
    30481 tatgagacag gaatggttct accagatttt ctggaagaag agctttctgt ttactatgct
    30541 ggagtttcct caaataacgg caaactagtt ggagcaggag gacgtcttta tttagtagaa
    30601 gcatacggtg agacgatcaa agaagcacag aaaaaaattt acaccgcgtt aatggataaa
    30661 acgacagatg gaacatttta tcgtacagat attggagaaa aagctgccaa tagcgaactg
    30721 atcgtgaagt aaatcagctg cttccattta taaaaaatac aacaaacata atcggaagcg
    30781 ccgattttag tgaagccttt tcataaagag tgtggtacac ttttgttgtg tatataaata
    30841 aggaggagtt cagatgaaaa aaattcaatt aggtacttct gatcagaaag cagctgcagt
    30901 gatcttaggc tgtatgcgta tcaacaatgc ggaagatcca gtaaaagtga ttgagacagc
    30961 ttatgaaaac ggcattgatt tttttgacca tgcagatatt tatggaaacg gtgaatgtga
    31021 agaaatcttt gcagatgctc tagcaaaaac atcaatcaaa cgagaagatc tttttattca
    31081 aacaaaatgt ggaatcgttc caggtgtgat gtttgatttt tctaaaaaac atatcattga
    31141 atctgtagaa aaaagtttga cccgtctgaa aatggattat gtggatgctt tacttcttca
    31201 tcgtccagat acattggtag aaccagagga agtagctgaa gcgtttgatg aattggaacg
    31261 gtcaggaaaa gttcgttatt ttggcgtcag caatcaaaaa ccaatgcaaa tcgaattgct
    31321 gaaaaaatat gttcgtcaac cattagtagc taatcagctg caatttggct tgaaacatac
    31381 tggaatgatc gatcaaggga ttcatgtcaa tatgacagat gaagcaagta tcgatcatga
    31441 cggtagtgta cttgattatt cccgattgca tgatatgacg atccaagcat ggtcaccata
    31501 ccagtatgga ttttttgaag gtgtatttat cgggaacgaa aaattccctg aattaaacca
    31561 aacattagaa gaactagcag ctaaatatca aacgacacca actggcttgg cttctgcttg
    31621 gattttacgc catccagcca atatgcaagt gatcgccggt acgatgaacg tagggcgaat
    31681 caaggaaatc gctcaagctt cagaaattaa tatgtctcgg gaagattggt acgaattgta
    31741 ccgtgctgct ggaaacattt taccttaaag attgtcataa gcaggtcaaa agggctataa
    31801 attaaaaaga cagaataact tgaaagctgt tttcagcttc gagatattct gtcttttttt
    31861 attgcaaatg gaaagagtat cattgtacgg atttcgttta ttagttattt ttgtttttaa
    31921 ggaaaagctg tgtccgatat tcttttggag aaatcccttg ttttttctta aataaataac
    31981 tgaaatagtt agggtcatta taaccaattt caaaagcaat atctgaaata cgatagtctg
    32041 attgctgcaa taattttttt gctaattcga ttctttgttc tgtcagataa tcgatgaatg
    32101 tttggttaaa cgattgagaa aaaatcgtac tgaaatgaga gggacttaat gcaacttgtt
    32161 gagcgaccca agttaaggac atatctggat cggtaaaatt ttctttgata aaatccaatg
    32221 catggttcaa aattggctga tatttgttcc ttgaaggctg tgagggcaga gcagttaaga
    32281 aagaaatgaa catataaact gtcgaacggt aaagcgagtc gtcggcgctg attgctaagc
    32341 gttgactggt ggatgtgcaa tgaaccattt cttgcgggat tttcttttct ttcaggcaga
    32401 catgctggag tagcttagtc aagacaaata aacgatataa tcgtgttcga gttggtgtgc
    32461 cttgtggttt cgtcaagtgt tcgatgtact tagatacttc gtttttcgat aatgaagaca
    32521 aatcaagtga gaggtcaaag taaaagacag aatcaactgg aagtttcgct tcttgaatca
    32581 aatcctcata gctgataatt tgttctttaa gggaactggc ggtttcaagt atcatctgcg
    32641 tgatctcaaa agatgcaggt aattcgctga ttctttctgc gacaggacca atggcgacag
    32701 aaaaattatt tttttgtccc ttttctaatt catggaccaa cgtttgggct acctgataac
    32761 tggtctcgat cacattttct tcaagagaat caactattaa aaatttgatg aatcgagagg
    32821 aaacagcaga gaagatcact tgttgattct ccctaaataa aaacttcaaa taatcactga
    32881 gatgagcgta atcttcaaag tctgtactga cttggtttgc tgctaataat acggtcattt
    32941 ttttccctgc taaagagcga ttgaaagatt cagcaagttt caatgtttca ctgatcgtaa
    33001 gtgagccttt gaacaaaccg ttcaagaaat gatttttttt caattcgaaa acaagatcag
    33061 aagaagtgtg cgtttctttt tgctgcaaat ctaaagcagt tactgctttt tctattgctt
    33121 gtttcaattc gctactttta atcggtttaa gcagataatc atctgcctgt acctggattg
    33181 ctcctttcat ataatcgaaa tcgtcgaatc cactaataaa tatgagacga gtccaaggaa
    33241 gtagtttcct tgcttctttt gaaaaagtca acccgtccat gaaaggcatt tggatatcag
    33301 tcagaatgat gtcgggctgt aagtcgataa ttgaagacag tgccatctca ccatctccag
    33361 cttcgccgac ataagtgatt ggataataag aaccaaggac agttatttgt tcccgcaagt
    33421 tattgcgaat gaggtattcg tcttcgatga taaaaacttt atacatgttt gtttcctcca
    33481 attgttttgg gagatagagg gatcatcagt gtgacagttg ttccttctcg gtatttgctg
    33541 tcaatcgtga taggtttttg atcatttccg taataaagaa ggagacgctt gtttacgttg
    33601 taaagtccgt agccgatact gaaatctgaa tcgatccctt tggctaactc tttacgtatt
    33661 tctgccagtt tttctgcagg gatgccaatc ccgttgtcta taactttgaa ttggatcatc
    33721 tcatcaattt tgcatgcggt aatacgaata aatcctttgt gacggacgta cttcgttcca
    33781 tggtatacag cattttcaat caatggctgc aagatcattt ttaaaataga aacaggaaaa
    33841 agtgtttctg gtatatcgat cttatagtca agcatatggc cgtatcttat ttttaagatg
    33901 actagataat cattgatatg gctaatttct ttttcaagag gaatccagtc ttgtcctttg
    33961 cttaaagacg tacgaaaaaa gccggaaagt gcatgggtca tagtcagagc ttcttcaaag
    34021 cagtcttgtt cgatcaaaga aataatggca tccaaactgt tataaataaa atggggagta
    34081 atctgagctt gcaatacacg aacttcactt tgtgctaaat ggtattgttt caaagcgttc
    34141 tcccgtaaag actggtttag atcttccgcc attttattca tgctagcggt aagtgtttgt
    34201 aattccagtg tttccggcag ggttaaacga aagccaaggt tccctgaaga aagttgttct
    34261 gacatctcga tcagctgatt gatcggttct tggacctttc tctctaaaaa gcgacgattc
    34321 tttttcgcat agaaaaaaat ggtactaaga actccaagtt ccactattgt taatagaatg
    34381 agcgagcgga caagtgtcgt attttttttg cttgctaagt cgatctccac acgaacaaaa
    34441 tcttgtaaga tatctgacag cagttgagtg acagaatcta cctgattgat gatctcttca
    34501 ttggtatcca taaaatcttt tgccaagata ttattgatca gctcgttttg ataatcttcc
    34561 agtgtatcga tcgtacgtag cgatacatct aacgtactaa tttctttaag tgtatttgta
    34621 ttatttttga tctctcgaat atcattccta agttcttcta ggatattttc ctcattgaac
    34681 tcatctacgg ttacttggcc agaaatcaag tcccacatac tttccaaaac tttttgatca
    34741 accttcaaag caattgcatc tgctttttgg atatttgcga tcgtctgttg ataaatcaaa
    34801 agctgttgcg aatataacag tatgctcagt agcaaaggga taacagcaaa tagaagcatg
    34861 gctttgtttg cttgggcaaa aatctgtttt atgcttaaat tttttgtcat gatacttctc
    34921 cagtcaataa tttctggttg ggatgttagc tatattctgc tgggaaaaga cagtctcgga
    34981 aataaaaata tctttaggta atgtttttcc tgtttgaata tttccaaaat aacgagtaac
    35041 ggtattggct acataccagc cgacatcagg tttacactcg actacacaat tgacgattcc
    35101 tgagcgtagc ttctcgatca tatcttcttg tccatcaata gttacaatga tcaaatcttc
    35161 tccaggtttg attgatgttt cttttatcgc agctaaagca ccgtgagtca tttcatcact
    35221 atggctgtat aatacatcga tttcggataa atcatttgtt tctaaatatc gtttcatctg
    35281 gtcttttcct ttcatccgaa taaaatcacc gacaattcgg tgaacaatag tcatcggagg
    35341 tttatggtcg atggttccat ctttttttag ggttggttta cgattgattg attccaaaaa
    35401 tccttcggag cgtagcttgg tcggtgtgga gttctctgaa ccagcaagtt ctaggattct
    35461 gatttcctgt ttctttgaat ttgtgaagtg attgctgaca aataaacccg cccgatttcc
    35521 ttctgctttg aaactggggc caatgtgggt gacgaaaagc tcgggatcat tcgtttgaat
    35581 atgtctgtcg acaagtatga cagggatccc tgctcgcttt gcttctttta atacagagtc
    35641 ccacccggat tcttgcaaag gagtaaaaat gatcatatcc actttatagg caatgaacgt
    35701 ccgtatatct tggatttgcc gttcctgatt catgtagcca tttcggtaga gaacttggta
    35761 atcttctttt tcaagcgcag tgataattga ttcagtatgc tttttccgcc agctactctc
    35821 cgttcctgat tgcgaaaaac caacgactag tgggtcaggt gcttcctttg tattaggtgt
    35881 acaccccatc atttggtaag cgattacaac aagggctaaa aaaaataaaa cacgtttcac
    35941 tttaaatcac tccatctgga tttgttagta ctatcttatc agataaaagg gaaaatcaaa
    36001 gtcaaaagga atcaatcgaa aaaaatccaa gaaacttaaa aaaatccaag acaaaaaaag
    36061 tacacctaaa aaatttaaag taaatcccat aaaaaaatca atgttaacgc tttctttatt
    36121 agctatactc ggtgtatcaa ttgaaatggg ggataaaaaa tgaaaaacaa atttagaaag
    36181 attgttggga ctatcgctat tttgaccgca agcattctat taagtgcgtg tggcaattca
    36241 ggatctgcag atgatagtac ggggtatgta ggaatcgcta tgccgaccaa atcagcagaa
    36301 agatggatcg ctgatgggaa caacatggtc tcggaattag aaaaactggg ttataaaaca
    36361 gatcttcaat atggagaaga taaagtagaa aatcaagtag cacaaataga aaatatgatt
    36421 acaaaagggg tagatacact tgtgattgct tctatcgatg gttcggcttt aactgatgtg
    36481 ttagctaaag caaaagaagc agatatcaag gtgatcgcct atgatcgttt actgatgaat
    36541 tcagaaaatg tcgattatta cgcgactttt gataattttg gagtaggtgt atcgcaagca
    36601 gcttatattg aagagcactt aggattgaaa gaaggaaaag gaccattcac gattgaatta
    36661 tttggtggct cgcctgacga caacaacgca ctaatcaatt acaacggagt gatgtctgtt
    36721 ttacaaccat atatggacaa cggtcagttg gtcgtgccat ctggacagac gagtttcagt
    36781 caaatcgcta cgcttcgctg ggatggttct acggcgcaag caaggatgga taatctgtta
    36841 agcgcaaatt atactgacca aacattggat gctgttttgt cgccatatga tccaataagt
    36901 ttaggaatca tttcgtcatt aaaaggcgtg gggtatggga gtgaaagtaa acctttacct
    36961 gtgattactg gacaagatgc gacagttgct ggcgttaaat cgatcattgc cggggaacaa
    37021 acacaaacga tctttaaaga cacacgtatc ttagcaaaaa atacgattga aatgatcaaa
    37081 gcaatcagtg atggagaaga ggttccggtc aatgatactg aaacttatga taatggggtg
    37141 aaaactgtcc ctacgtattt agcaaataca gtatctgtcg ataaagataa ttatcaagca
    37201 gagctgatcg acaccgatta ttataaagaa tccgatctga aaaattaaga ggaaccaaac
    37261 aaggaactaa agagatctaa gtcagagaga ggataagaat tatggcggat tgtattttaa
    37321 aaatggcaga tatcaccaaa aaattttctg gaatccgagc gttagattgc gtgaatctga
    37381 tgattgaacg tggggaaatc catgcattgt gcggagaaaa cggagcggga aaatcaacat
    37441 tgatgaatgt cttgagcggc ttgtatccat atggctccta tgacggggaa atcttttatg
    37501 atggaaagct gtgtcaattt aaagatttga aagatagtga agaaaaaggc atcgtcatca
    37561 ttcatcaaga attagcacta agtccttatc tttcaataaa agagaatatt tttctcggaa
    37621 atgaacaagc agaaagaggc gtgattgatt gggaactgac agaaaagaaa acatatgaac
    37681 tgctgaataa agtcggtttg tctatcgacc cgaatcttct agtatcccag attggcgtag
    37741 ggcaacagca actagttgaa atagctaaag cattatcaaa atccgtccgt ctgttgattt
    37801 tggatgagcc taccgctgca ttgaatgaag aagaaagcgc gaatttgctg agattgatcc
    37861 gagagttgaa agagcaaggt gtaacctcca tcattatttc acataagctg aatgaaatcg
    37921 tggatatcgc agatcgaatc accattttac gggatggaca gacgattgag acactagaaa
    37981 atgaaatgat cagcgaagag catattattc gcgggatggt gggcagagat ttagttgatc
    38041 gttatcctga aagaaaaccg gatattggag aagtttattt taaagtagaa gactggacgg
    38101 tctatcaccc tcttgatcac gagcgaatca taaacgacca catcaacttg catttgaaaa
    38161 aaggagagat tgtaggaata gctggactga tgggagcggg cagaactgaa ttcgctatga
    38221 gtctatttgg acattcttat ggaagcaaga tatccggacg tatattaaag gaaggggcag
    38281 agatatcagt taagaccgtt ccttcagcca ttgcaaacgg actcgcttat gtctcggagg
    38341 accgaaaatc tttaggactg aatctgctga tggatattcg ggaaaacgtg acgctgagca
    38401 gtttggataa aataagtcaa aaaggagtat tagataaaga aaaagaagta actgaggcag
    38461 aaaatttccg gaaaaagatg cgtataaaaa caaactccat ccatcaaatt gtcagtagtc
    38521 ttagtggtgg gaatcaacaa aaagtcgttt tatccaagtg gctgatgaca caacccgata
    38581 ttcttttttt ggatgagcca acccgtggga tagatgttgg ggcaaaatat gagatctaca
    38641 cgattatcga agaaatggca gcaaatggca aagctgtctg tcttatctct tctgaacttc
    38701 ctgaaatatt agggatgtgt gatcgaatct atacgatgaa cgaaggacgt attacaggag
    38761 aagtctcgcg agaagatgca gatcaggaag tcttgatgaa attaatgacg aaagaagagg
    38821 tgggttaagt tggaacaggc aaaaaatctt acggataaaa aagagaaaaa actaaatttg
    38881 aaaagtagag cagctgatct ttttggtcgc tacagcatgg tgatcatttt agcgggacta
    38941 cttattgcgt ttcaattgat gacggatggt attttctggc gtccattgaa tataactaac
    39001 cttgttctgc aaaatagcca tatattagta ctagctgcag ggatgttatt agttgtgctc
    39061 ttaggtcatg tggatttgtc ggtagggtca gtgatggcgt ttgttggtgc aattgcgggc
    39121 atgatgatgg tcaatcgcca aatgaacccg tggatgacag ttatcgcatg tcttgcagta
    39181 ggcgggctga tcggcgcatg gcaaggtttt tgggtagctt atgcagggat accggcgttt
    39241 atcgtgactt tagcaggatt gctgatgttt cgtggactga cacaagttgt tctaggaggc
    39301 caatctctag caccttttcc agctgttttc caaaaaatgt ctacgggtta tttaccagac
    39361 ttaagcgaag gaagcagcct tcacttcttg acattgattt taggaatgat catcacgatc
    39421 gtgttagttg ttggtcaatg gcgaacaaga aatcgtcgga aaaatcattt atttgaagtg
    39481 gaatctccaa cagctttctt ggtaaaggcg gtatttacag cagtcgttgt actaggaata
    39541 acttatatct ttgcctctta tcaagggtat ccgattattt tgatcattct tggtttgatc
    39601 gttgcagcat atgggttttt gacaaataaa acagtagcag gtcgacagat ttatgcgacg
    39661 ggcggaaatc gaaaagcggc tgaactatca gggatcaaaa caaagcgaat cacgttctgg
    39721 gtattcgtca acatgggagt catggcagct ttagctggat tagttttagc tgctcgattg
    39781 aatgcggcaa cacctcaagc aggtacatcg atggaactgg atgcaatggc ggcagtatat
    39841 tttggtggtg cttcgacttc cggcggcatt ggaacaatca tgggtgcgat tgtcggtggc
    39901 ttggtcatgg gtgtattgaa caatggcatg tcgattttag gcgtcggggt agactggcag
    39961 caggcaatca aaggattgat cttgctttta gccgttgtgc tagacttgta taataagaaa
    40021 aaacgaacaa cgtaggagaa cgccggttat tcagaaaaaa gactgtgaca agacatttgt
    40081 cacagtcttt ttttcgctaa aagcgaacat tcattgtttc gtttgtatag actaaaaata
    40141 atatatgttt ttttaccatt cgtataaatt ctctttttgc tcttgatttt tttgcttaat
    40201 tgagttaaat tacatagggc gctaaaaaaa gccttgatta aattaataat gttcgtgttt
    40261 tgctggatgt gaacatcagg aggataatgt aatgaaagta tttgattacg aagatattca
    40321 attaattcct aataaatgta tcgtaaatag ccgttctgaa tgtgatacaa cggtcacatt
    40381 aggtaaacat acattcaaaa tgccagtagt gcctgccaac atgcagacga ttattgatga
    40441 aacaatcgca gaattcttag cggaaaatgg ttatttctat atcatgcatc ggtttgatga
    40501 agcagctcga attccgttta tcaaaaaaat gaaaaagcgt ggattgatta cctcgatcag
    40561 tgttggcgtc aagaaagagg aatattcctt tatagagaag ttggcagaag aatcgctaaa
    40621 tcctgattat atcactatcg acatcgcgca tggacatgct aactcagtta ttgatatgat
    40681 tcagcatatt aaaaaatatt tgcctgaaac ttttgtgatt gccggtaatg ttggtacacc
    40741 ggaagccgtc cgtgaattgg aaaatgcggg cgcagatgca acaaaagtcg ggattggtcc
    40801 aggaaaagta tgtattacaa aaatcaagac aggctttggg actggaggct ggcaactggc
    40861 agctcttaga tggtgtgcga aagcagctcg caagccgatc atcgctgacg gtgggatcag
    40921 aacacatggc gatattgcaa aatctgtccg ttttggtgcg acaatggtca tgattggttc
    40981 attgtttgcc ggtcacgagg aatctcctgg cgaaacaaaa gtcgaaaatg gaatcgtgta
    41041 taaagaatac tttggttctg cttcagaatt ccaaaaaggc gaaaaacgaa atgttgaagg
    41101 aaagaaaatc tggatccaac acaaaggatc tttgaaagat acactagtgg aaatgcaaca
    41161 agaccttcaa tcatctattt cttatgctgg aggaagagat ctggaagcca ttcgaaaagt
    41221 tgactatgtc atcgtgaaaa attcgatttt taatggagac acaatctaaa aaataaataa
    41281 aatattagga tgttgtgaaa aagttgtcct gcattaagta ataagaacag ccaaacaaaa
    41341 atagttcttt atatgttttg tttggctgga attattatcg cagatacaag cttttgaaca
    41401 tcattattca gaataaggga ctgtgataag atttttgtca cagtccctta tgttttgcaa
    41461 aaaaagtttt tcttttttct cgtaaattcg gtaaaatcaa aagaaaaaat agacaataga
    41521 aaagagaagc ttatgtggga aaaagtagag aaaaaacaag gaccgattta tcgtcaaatt
    41581 atggaacaga ttatgagaga aatcgaaaac ggaaggatca atcctggaag tcaacttgaa
    41641 tcagaacgaa aactagggca actttttggt gtgaatcgtt ccaccgttgt ccatgcattg
    41701 gaagagctta gggagcttgg ggtactaacg agcaaacgtg gcagcggatg gtttgttaat
    41761 cagactgaat gggggaaatt cgctgttcca agaatcgatt ggcggcaaat gatctctcct
    41821 cgttatgagc agacggactg gtatgagcaa aaaataagag aagcaaagca gatccaaaaa
    41881 acatcgtttc ttgatttata cgctagcgag atgccagatg agcttttgcc gaattttgag
    41941 tttccttctt attctcttga agaaatctta gcggaagaaa aagaaatgga tcttttaggc
    42001 tatactccgt tacgtaacaa aatcaaacgg tatttggaaa gtaaatttga attggcattt
    42061 tcccccaacc agcttcttgt gactggtggc ggtcagcaag caatcttttt gattttacaa
    42121 acgattcttt cagctggaga ggggatagct gtagagtcac cttctttttt ttaccgatta
    42181 cctcttttca aagcttcagg aacgcgttta tttgggatac cgatggatga agaagggatc
    42241 gacttagaaa aactgaaaga atcgatccat aaaaataaat tgaaagcagt gcttatcaac
    42301 ccgaattttc aaaaccctac aggaaaagtg atgagtcaaa aacgacgaga aaagctagta
    42361 gaattgtgtc gacagtatcg gcttccaatt attgaagacg atgtatttac ggatctttca
    42421 tttctaaacc aagaagaagt ccttcctata cgaacgattg atccggataa tgtgttatac
    42481 gtcggttcgt tatctagggt tttaggaaaa acaacgaaaa tcggttgggt gataggacct
    42541 gagactttta tctcgcaact agctcaagcg caagaaatga tggagttttc gatgaatatc
    42601 cttacacaaa tcacagtggc taatgtattt aatgaagcaa ttgatttgaa aatgcaagat
    42661 gtaagaaaaa agttgaaaga aaaaagccga ctgataagcg cttggggaga ttcacaatcg
    42721 tttttcaagc ttcattcaat tttaggtggt tactatgcct ggattacttg ggatgggaaa
    42781 aaactagacc ccgaactagc agaaaaagta gtagatatgg gtcttggaat tgcaccaagt
    42841 ctactttttg ggcaagatat aaatggaatc agaatcaatt acagtaggct ggatgaaaac
    42901 caaataccgt tgttttattc aaagatgaaa attctagaaa agtggttaac agaataattt
    42961 cacaaagtaa atagattaaa gaatataaca gcttggattt aaatttcaag ctgtttcttt
    43021 tttttcggaa aaagaatacg atataaagaa actaagaaaa aaccattatt tgtacgtccg
    43081 ttcacgatgt gtaaataaaa taaatgtttg caacaaaact gtataacacc aacgttttta
    43141 aatagaaaca gatggttgta agtttgatta gaaaatagaa aaaatgcaga tgaaaaataa
    43201 aacgctttcc ttattgtgat tttattcact aatgctgtta atatgcggat tggcataatt
    43261 tcctttcatc gtgaaaattt gtgcttttag cttgtatttc tcataaatcc atgataattt
    43321 attaatgaag acggttaaat agtgatacag ttttattcac tgtttcaatc gcagaatgaa
    43381 gagattttct tatagatgag agaggaatgt gtgacaaatg gcaaacaaaa aagcaccgat
    43441 cgacttccaa gcactgctag atgaagtcaa tgcagatttc ccagtatatc aagcacttga
    43501 tcaagatgga aacgtagtta acaaagattt agtacctgat ttatccgatg atgaactcgt
    43561 tgaattaatg actcatatgg tatggtctcg tgtattagac caacgttcaa cagcgctgaa
    43621 ccgccaagga cgcctaggct tttttgcacc aactgctgga caagaggcaa gtcagctggc
    43681 tagtgcttac gcatttgata aagaagatgt tcttcttcca ggttatcggg atgtacctca
    43741 actagtaaaa catggtttac ctttatctca agctttttta tggtctcgag gacatgctgc
    43801 aggtaattct tatccggaag aattgaaagc tctaccacca caaattatca tcggtgcaca
    43861 atatgtacaa gcagccggtg ttgcattagg tttgaagaaa cgaaacaaga aaaatgtcgt
    43921 cttcacttat actggtgatg gcggttcatc acaaggtgat ttttacgaag caatcaattt
    43981 cgctggcgct taccatgcaa atgccgtatt ttatatccaa aacaacgggt atgcgatctc
    44041 tacacctcgt gaaaaacaaa ctgcagcaaa aacacttgca caaaaagctg tcgcagctgg
    44101 gattcctggt attcaagtcg atggaatgga tccgcttgca gtatatactg tatctaaaat
    44161 ggcacgggaa tgggcgatca gtggaaatgg accagtccta atcgaaacac tgacttatcg
    44221 ttatggacca catacactat ctggtgacga tccaacccgc taccgtacaa aagacacaga
    44281 tgacgattgg cagaaaaaag atccattgat ccgcttccgt aaatatttga cggaaaaagg
    44341 actttggtct gaagaaaaag aagaacaagt catcgaagca actaaagaag aaatcaaagc
    44401 agctattgct gaagccgaca aagtaccaaa acaaaaggtt tcagatttct tgaaaaacat
    44461 gttcgaagta tctccacaat caatcaaaga acaaattgca atttatgaag cgaaggagtc
    44521 gaaataatca tggcacaaaa aacaatgatc caagcaatca cagatgcctt agcacttgaa
    44581 ctagaaaatg acgagaatgt cgttgttttt ggtgaagatg ttgggaaaaa cggaggagtt
    44641 ttccgggcaa ctgaaggatt gcaggaaaag ttcggagaag accgagtatt cgatactcct
    44701 ttagctgaat ctggaatcgc aggattgagt ttcggtcttg cgttagaagg atttcgtcca
    44761 gttcctgaaa tccaattctt tggttttatt ttcgaagcaa tggacgaagt agtagctcaa
    44821 atggctcgta caagatatcg aatgagcggc actagaaatc tacccatcac aattcgttcg
    44881 ccatttggtg gcggtgttca tacaccagaa ttgcattcgg ataatttaga aggtttgatc
    44941 gctcagtctc ctggtattcg agtagtgatt ccttccaacc cttatgatgc aaaaggacta
    45001 ttgatttctt ctattagaag taacgatccg gttgttttcc tagaacacat gaaactatat
    45061 cgctcattcc gagaagaagt gccggatgaa gcttacgaag ttcctttgga taaagcagca
    45121 gttactcgtg agggaacaga tgtatctatt atcacatacg gagcaatggt gcgtgaagca
    45181 atcaaagcag cagacaatct tgcaaaagaa aatatatctg ttgaaatcat tgatcttcgt
    45241 acagttgcgc cattagatgt agaaacgatc attcaatccg tggaaaaaac tggtcgcgta
    45301 gttgttgtcc aagaagcaca acgacaagcg ggcgttgccg cgcaagtagt ttctgagatc
    45361 tcagaacgcg ctatcctttc attagaagcc ccaatcggac gagtttctgc acctgataca
    45421 gtcttcccat ttggacaagc agaaaatgtc tggttgccaa atgcaaaaga tatcgaagac
    45481 aaagtaaaag aaatcgcgga attttaagaa aagaagggac gaaacaaagt catggcttat
    45541 caatttaaat tgccggatat cggtgaagga atcgcagaag gtgaaatcgt caaatggttc
    45601 gtcaaaccag gagatacgat caatgaagat gatacattac tagaagttca aaatgacaaa
    45661 tcagtagaag aaatcccgtc acctgttaca ggaacggtta aaaatgtgat tgttccagaa
    45721 ggtaccgtag caaatgttgg ggatgtatta gtagaaatcg atgcaccagg acacgaagac
    45781 aatgaagggg acagtggtgt agccgcagaa tcccaaactc cagctaaacc ggctgctgaa
    45841 cccactgttg atacagaatc agctggatcg tcaagcgaag gagtcttcca atttaagtta
    45901 ccggatatcg gtgaaggaat cgcagaaggt gaaatcgtca aatggttcgt caaaccagga
    45961 gatacgatca atgaagatga tacattacta gaagttcaaa atgacaaatc agtagaagaa
    46021 attccgtcac ctgtcacagg gacagttaaa aacgtgattg ttccagaagg cactgtagca
    46081 aatgttgggg atgtattagt agaaatcgat gctcctggac acaatagtgc accgtcaact
    46141 agtgcgccat cagctgaagc accaaaagaa aaagtggaaa catcaggttc agcaagtgtc
    46201 gttgaagctg ccgatccaaa taaacgtgta ttggctatgc catctgttcg tcaatttgcc
    46261 cgtgaaaaag atgtagatat ctcacaagta actgcaactg gaaaaggtgg cagagtaaca
    46321 aaagaggaca tcgaaaactt cttggcaggt ggtccaagca gtgcaccagc gaaatcagaa
    46381 gcgccagaag cggcagcacc gaaagaagca gctcctgcag cagaaagcaa accagctgca
    46441 ccagcaaaac cattcaagtc caacttagga gacttggaag aacgtgtagc aatgacgcca
    46501 acacgtaaag ctatcgctaa agcaatggtc aacagtaagc atacagctcc acacgtgaca
    46561 ttgcatgatg aagtagaagt gtctaaacta tgggataatc ggaaacgatt taaagaagtt
    46621 gctgctgcaa atggtacgaa acttactttc ttgccatatg tcgtgaaagc tttaacagcg
    46681 actgttaaga aatacccagt attgaatgca tctatcgacg atgcaaacca agaaatcgtt
    46741 tataaacatt actacaatat cggtattgca actgatacgg atcacggact atatgtacca
    46801 aatgtgaaag atgcagaccg caaaggaatg tttgctattg cagacgaaat caatgagaaa
    46861 gcaaaattag ctcatgacgg caaactatct gcagaagata tgcgcaatgg aacaattacg
    46921 atcagcaata tcggatctgt aggtggcggt tggtttacac cagtcatcaa ctatccagaa
    46981 gtagctattt taggtgtagg aacgattgca caacaaccaa tcgtaaatgc tgaaggcgaa
    47041 atcgttgtcg gtcgtgtgat gaaactgtca ttaagtttcg atcaccgtat cgtagatgga
    47101 gcaactgcgc aacaagcaat gaataatatc aaacgtcttt tagctgatcc agagctatta
    47161 atgatggaag gatgataaac aaatggtagt tggagatttt gctgtagaat tagatacagt
    47221 tgtaattggt gcgggacctg gtggctatgt agctgccatc cgcgctgctg aaatgggaca
    47281 aaaagtagca atcattgaaa gagagtatat tggcggtgtt tgtttgaatg tcggctgtat
    47341 tccttcaaaa gcactgatag ctgcaggaca ccattatcaa gagtcactag attctgaaat
    47401 gtttggagta actagtgaaa acgtcaaact agattttgca aaaacacaag aatggaaaga
    47461 aaacaaagtt gtccatacgt tgacttctgg tgttggtttc ctattaaaaa aacataaggt
    47521 tgaaacaatc gaaggagaag cgttcttcgt agatgatcac acactacgcg tcattcatcc
    47581 agattcagct caaacttatt cgttcaataa cgcaatcatc gcaacaggtt cccgtccaat
    47641 cgagattcca ggatttaaat tcggaggacg tgtgttagat tctactggcg gattagcatt
    47701 gaaagaagta cctaagaaat ttgtgatcat cggtggcggt gtgattggtg ccgaattggg
    47761 cggagcgtac gcaaacttag gagcagaagt aacgattctt gaaggctcac cacaaatttt
    47821 accaacttac gaaaaagatc tagtaaaatt agtagaagat gatttcaaga aaaaaggcgt
    47881 aacagtcgta acgaatgcaa tggcgaaaga agcagtcgat aacggtgaca gcgtgacagt
    47941 gaaatatgca gtggacggga aagaagaatc cgttactgct gattatgtga tggtcactgt
    48001 aggtcgtcgt ccgaatacag atgatatggg cttagaacaa gcgggtgtcg aagtaggaga
    48061 acgaggattg atcacggtgg ataaacaagg acgtacgaac gtacctaaca tctatgccat
    48121 cggtgatatc gttccagggg cggcattagc acacaaagcc agttatgaag caaaaatcgc
    48181 tgctgaagca atctctggca aaaaagtagc tgtagattat aaagcaatgc cggcagtagc
    48241 cttcactgat cctgaattgg cttctgtagg tatgacgatc aaagaagcaa aagatgctgg
    48301 tatcgaagca aaagcatata aattcccatt ttcagggaac ggtcgggcat tatcgcttgg
    48361 taaaacagaa ggattcatcc gtctagttac gacgatcgaa gataacgtgc ttattggcgc
    48421 gcaaatcggt ggagtcggcg caagtgatat ggtctctgaa ttagctttag caatcgaatc
    48481 aggaatgaat gctgaagata tcgccttgac gattcatcct catccatctc ttggcgaaat
    48541 cgtcatggat gcttcggaat tagcattagg tttaccaatc catatttgat ttgaataata
    48601 caggcgaaac gcaactgaaa aatcagttgc gttttgtttt ttagaattat caataatatt
    48661 attatgtaaa cttaaaaata tgacaacttc ttggtttatt accacttcct atgctacact
    48721 gaagaggaat atgaaatagg agagttgaag atgcaagaat atcagtatcc acttgattta
    48781 gattggacaa cagacgaaat ggtcattgta atgaagatgt gggaagatct ggaacgtgcg
    48841 aatgaaaaag ggataaacaa tgaagctttt ttgaagactt atcagcagtt taaaactgtt
    48901 gtcaaatcca ttggtgagga aaaacgattg ggacgggaat ttgaaaaagc ttccggttat
    48961 tctttatatc gtacagtgca gcaagcaaaa aaaaatccaa acaaaacttt gagaatgaac
    49021 gggtgagata aatggaacag atgattgatc atatcaagtc ctggcttgaa gaagctggtg
    49081 agaaaatcaa atcttctcaa caaacattaa caatctcgca aaaatcaaat cgaacggatc
    49141 tagtaacaaa tgtcgatcga aacatccaga attttctaat cgaaaaaatc cattcctttg
    49201 atccagaggc gaaaatattg gcagaagaag aagggtgtaa tcaattatcg gatctcgatg
    49261 gaagagtctt tatcatcgat cctattgatg gcaccttgaa ttttgtgatt gaaggggaaa
    49321 atttttgtat tatgctcgct gtttatgaat ccggcgttgg aaagcttggt ttcatttatg
    49381 acgtgatgag aaatgaactt ttttggggtg gaaaaggaat aggcgtgtat aaaaatgatg
    49441 agccattgcc tagaccagca aataaatcct tatcggaagg cttacttggt gtgaatagtt
    49501 atcttttcgg tcataataaa tatggaatcc gagaaattgc ggaaagcagc atggggacac
    49561 gtatgtacgg atgcgctggt cttgaactag tggcaatctt gaaaggaaac cacattgctt
    49621 atatttcaaa ccttagccct tgggattatg cagcgggaaa tgtcttattg gaagagtttg
    49681 gtatggtaac aagcggtctt tttggtactc ctttgacatt tgaaaaaagg gaatactatt
    49741 tagcagggac ttcttgtgca tatgcagaaa tcgcagacct tttgaaaaaa gaaggctgaa
    49801 aaaaaggtag aaatcattca caaaaatgat aaaaacgagc aaaagattaa ttttctcgaa
    49861 aaaatgaaaa cattttacta tatttcatga aaaaagtctg ctataattag cagtcgagta
    49921 aaatttgccc tgtcataaag aaccaataaa aaaggagtta gttacattga attatagaaa
    49981 cgatatccgt aacgtcgcaa ttattgccca cgttgaccac ggaaaaacaa cattagttga
    50041 tgaactatta aaacaatcag atacattaga cgcacatacg caattgcaag aacgtgcgat
    50101 ggactcaaat gcgctagaaa aagaacgtgg tatcacgatt ttagccaaaa acacagctgt
    50161 agactataaa ggtattcgcg taaacatcat ggatactcca ggacacgcgg acttcggtgg
    50221 cgaagtagaa cggatcatga aaatggttga cggcgttgtg ttggtcgtag atgcgtatga
    50281 aggaacaatg cctcaaacac gttttgtatt gaaaaaagca ctggaacaac atatcacacc
    50341 aatcgttgtc gtaaacaaga ttgacaaacc ttctgctcgt cctgagcatg tcgtagatga
    50401 agttcttgaa ctattcatcg aattaggagc agatgatgat cagttagact ttcctgtgat
    50461 ttatgcatct gcactaaatg gtacgtcaag cttgtcagat gatccagcag atcaagaacc
    50521 aaccatggca cctatttttg atacaattat tgaaaaaatc cctgctcctg tcgataacag
    50581 cgacgaacca ttacaattcc aagtttcatt acttgactac aacgattatg taggtcgtat
    50641 cggaatcgga cgtgtattta gaggaacaat caaagtagga gaccaagtag cattaattaa
    50701 attagacgga acagttaaga aattccgtgt aacgaaatta tttggtttct ttggactaaa
    50761 acgtctagag attcaagaag caaaagctgg agacttgatt gctgtctcag gtatggaaga
    50821 tatctttgtc ggtgaaacag ttactcctgt ggatcatcaa gatgcgttac ctatccttca
    50881 cattgacgaa ccaacattgc aaatgacttt cttagttaat aattcaccat ttgccggtcg
    50941 agaaggtaaa ttcgtaaccg ctcgtaagat tgaagaacgt ttgatggcag aacttcaaac
    51001 agatgtatcc ttacgagttg aaccaacaaa ctctccagat gcatggacag tttctggtcg
    51061 tggagaattg catttgtcta tcttgattga aaacatgcgc cgtgagggat acgaattgca
    51121 agtatctcgt ccagaagtta ttgaaaaaga aatcgacggt gtaaaatgcg aaccgtttga
    51181 acgcgtgcaa atcgatacac cagaagaata tatgggtagt gttatcgagt ccttgagctt
    51241 acgtaaaggt gaaatgcaag atatggtcca tacaggaaac ggacaaatcc gtttgacatt
    51301 cttaacacct gcccgcggtt tgattggtta ttcaactgaa ttcctttcaa tgacacgtgg
    51361 ttacggaatc atgaaccata cttttgacca atatctgcca atgcttcctg gtcaaattgg
    51421 cggacgtcac caaggggcgc ttgtttcgat cgatacagga aaagcaacga cttattctat
    51481 catgagtatc gaagaacgcg gtacagtctt cgtagaacca ggtacagaag tatatgaagg
    51541 aatgattatc ggggaaaaca gccgtgataa tgatttgact gtcaacatca cgaaagcaaa
    51601 acaaatgaca aacgtacggt ctgctactaa agaccaaaca tctgtcatca aaaaaccaaa
    51661 acaattgacg ttggaagaat ctttggaatt cttgaatgat gatgaatatt gcgaagtaac
    51721 accagaaagc attcgcttga gaaaacaaat tttagagaaa aatgctcgtg aaaaagcatc
    51781 taaaaagaaa aaataatctg taaaaggatg aagagagaaa ccagcttgtt ataaaaaata
    51841 gctggtttct ttttttgtcc aactcttttc tgtggagaag tcatctttgt caatggacat
    51901 aaattcagaa aatacgccga aagacgttta aaagactttt aaaaaactta tttctagaga
    51961 gtttttgttt cataataaga taatgtttca cgaaaggaga tcaacttgtc cttcgaagaa
    52021 aatcgaagta aaatggcaag agaaaggagc gggaagatga acgacttatt aaaacaatac
    52081 tttggttatg atgagtttag acctggtcaa aaagaaatta tccagaaagt cattgatcag
    52141 gaaaatgtat taggaatcat gcctaccgga agtgggaaat caatctgtta ccagcttcca
    52201 gctttactat tagatgggct tactgtggtt gtgtcccctt taatctcttt gatgaaggat
    52261 caggtggacg cggcgaatca attaggtata cccgccactt ttatcaatag ctcgttagat
    52321 ggttacgaaa cagctagacg cttccaagaa atcgatagac agcagtaccg tttactttat
    52381 atcgctcctg aacgttttat catgccggat tttatccaag caatgaagcg atggaacgtc
    52441 cgtatgatcg ctattgacga ggcacattgt atctctcagt gggggcatga tttccgccca
    52501 agttatttac agatggcgaa ccagctagat caactgccaa atcgtcctgt aattgttgct
    52561 ttgacagcta cagcgactgt gcaagtagct gctgacataa aaagactttt aaaaattcca
    52621 gaaaacaacc atattcagac tggttttgaa cgagaaaatc tacgatttca agtaattaaa
    52681 gatcaaaaaa aagaacaata tctaatcgaa tatttaaaga tcaacaaaaa tcaatctggt
    52741 atcatttatg cagcaacgag aaaagaagta gaccgtctct atcatttgct taaaaaattc
    52801 gatttttccg tgggtagata tcatggtggt ctaaatgaga atgagcgaac agaaatgcaa
    52861 gaagcctttt tatacgatcg cttgcagctg attgtagcga caaatgcatt tggtatgggt
    52921 ataaataaaa gcaatgtccg ttttgttatc cattatcaaa ttcctggctc tctagaagca
    52981 tactatcagg aagctggtcg ggcgggaaga gatggtcttt ccagtgaagc catcctgctt
    53041 ttctctcctc aagatatcca agtccaaaag ttttttatcc agcagtccca aagagaagaa
    53101 gggcaaaagc aaaaagaata tgaaaaatta aaagcgatga cagaatacgt gtatattgag
    53161 tcctgtcttc agcaatacat cctaaactat tttggcgaga caagcagtcc gtgcaaccgt
    53221 tgtggtaact gtttagatga cagagaaata gtagaagtaa cgacagaagc acagatggtt
    53281 ctttcttgct taaaacgtat gggtgagaac tatggtaaac aaatgctgat gaaagtctta
    53341 gcaggatcga aagaacaaaa gctgcaagct ttaggttttg gacatttatc tacctatgga
    53401 ctgatgaaaa accaatcgca aaaagaaacg atgcagttaa ttgaatattt gatatcgaat
    53461 ggctatcttc tgaccatcaa tggtgagtat cctgttttga aagtaacaga aagagggata
    53521 caagtattga aaggacaaga atctgtttat cgaaaggaac ccaagaaagt ccagcagcta
    53581 tcggatgaag aaaccgatac actatttgaa gtgctgcggg aactgcgaac ggatctagca
    53641 tcagaagcag gcgttccccc gtatgtcgta ttttcagatt ccacattgaa agaaatgagc
    53701 cgaatacgtc catcaagcag attggagatg cttcagataa aaggagttgg ccaaagcaag
    53761 cttgataaat atggcgaagc ctttttatct cggataaaaa atgcggactc taacgttctg
    53821 gataagtaga aagaacgaat acgtaaaaat gaataaaaac aactggttaa atctgttttt
    53881 tttgctcctt atgaaaatca aatatgttat aattaaggaa ttagaatggg aggtaagcac
    53941 agatggattc tttgtcgaaa aaacttgcga tccaagtatt aaatgatgaa gcagaacgga
    54001 tcaaacgatt aatccgaaat caaaacaaca gtctctgtat ctcacaatgc aaagccttcg
    54061 aagaagtggt agatacacag atgtacggct tttcccggca agtcggtttt gctgtccgta
    54121 taggagtaat cggaaagaac gagggccagc tgatgttaag tgagctagaa cgagaattga
    54181 atcagttata tagtgaggtt taccaagaaa tttatgataa aaaagaagtt ggcaaggagg
    54241 aatagacttg cctaagaaag tgaaaaaaag gcatctatta gactatagca tcttgattcc
    54301 ctatttaatt ttatgtgtat tgggattgat catggtatat agttctacgt cgtatctatt
    54361 attagaaaac ggacaaaatc cgtccgcatc agtcattaac cagagtattt tttgggtgct
    54421 tagcttgatt gctatcgcac tactttataa aatgaaaaca gatgttttga aaaaccagcg
    54481 tttgatcatg gcagccatcg cagttttgac gattttgctg ctgattgttg tgttttttgg
    54541 taaggagatc aatggggcga aaggttggct gcaaattgca ggattctcga tccaacctgc
    54601 cgaataccta aaaatcattt ctatctggta cctgtctctg actttatcta aaagacaaaa
    54661 cagtgtacaa aaagattttc ttgggactgt gaaacgtcca cttgcaatgg ttatcggact
    54721 tactgcatta gtcgctattc ttcccgattt tggtaatgcg gcagttatct ttttgattat
    54781 tttggttctg cttttagcca gcggagtcaa ttatgtgtat actttgattg taggagtagg
    54841 aggcttttgt ctaagtactt ttacgatctg gctgatcaat atcacaaacg gaaagatctt
    54901 acccggacgt ttacagtata tctataatcg atttgctatt taccaaaatc ctttctcaga
    54961 cgaattaaat aaagggcatc aactagtcaa cggctactat gccatgttca acggaggact
    55021 atttggcagg ggattaggta acagtatcca gaaaaaagga tttttacaag aagcacaaac
    55081 cgattttatt tatgcaatcg tagtggaaga actgggagtg attatgggga ttttgatatt
    55141 agcgttgcta ttctttatga ttgtgcgcat tgtccttgta ggtatccgtt caaaagatcc
    55201 gttcaattcg cttttgtgca ttgggatcgg cgcaatgttt ttgatccaag tatttgtcaa
    55261 tcttggagga attactggcg ttattccttt gacagggatc acgttcccat ttttaagtca
    55321 aggaggctcc agtttgctga tgctttcgat ttgtgtcggc tttgtcctca acatcagtgc
    55381 ggatgaaaaa cgcaagagct taggtttata ataaaaaaag atgtaggagt ggttcgatga
    55441 aaaaagtttt agttgctaac aggggtgaaa tcgctgtacg tgtatttcgt gcttgtacag
    55501 aattaggaat taaaactgtt ggaatctatg cagaagaaga tgaatattcc gtacaccgtt
    55561 tcaaagcgga tgaagcatat ttagtaggac aaggaaaaaa accgattgat gcgtatctag
    55621 atattgaagg gatcatttct atcgcgaaag aatgcggagc tgacgctatt catccgggtt
    55681 acggtctttt gtcggaaaac ttgaattttg cgcaacggtg cgaagaagaa ggcatcattt
    55741 ttgttggacc caaactgcat catttagata tttttggaga taaaatcaaa gcaaaagctg
    55801 cagctatcga agcggggatt gcttcgattc caggcacaga tggaccaatc gcttctatag
    55861 acgatgcctt ggagtttgcg aaacaatacg gttatccgat tatgatcaag gcagctctag
    55921 gcggcggcgg acgcggcatg cgtgtagctc acgatgaaaa gagtgcaaga gagggctacg
    55981 aacgtgcgaa aagcgaagca aaagcagcct ttggtagtga tgaagtgtat gttgaaaaat
    56041 acatcgctaa tcctaagcat atcgaagtac agattctcgg ggatacgcat ggaaacgtca
    56101 tccatctttt tgaaagagat tgttctgttc agcgccgtca ccaaaaagta gtagaagtag
    56161 cgccttgtgt ctctatgaat gaacagcaac gtcaaaaaat ctgccaggca gctgtccaat
    56221 tgatgaaata tgtcgggtat gtcaacgcag gtacagtaga atttcttgtt gaaggagatg
    56281 atttttactt tatcgaagtc aaccctcgcg tccaagtgga acatacgatc acagaaatga
    56341 ttacagatat tgatatcgta acaacgcaac tgttgattgc tcaaggtttg gatctgcata
    56401 aagaaatcgg attgcctcag caagaaggaa ttaaattaaa tggttcagcc attcaatgcc
    56461 gaatcactac agaagacccg ttgaataact ttttaccaga cacagggaaa atcgatacgt
    56521 atcgttcacc tggtggtttt ggcgtacgtc tggatgtcgg aaatgcttac gcgggctatg
    56581 tagtgacacc gtattttgat tcattgctag taaaagtatg tacgcatggt gctacctttg
    56641 aaacagccat tcagaaaatg gaacgttgct tgaaagaatt tagaatccgt ggagtgaaaa
    56701 caaacattcc atttatgctc aatgtcatca cacatcctga attccaatca ggaaatgcaa
    56761 aaacaacctt cattgatagt acagcaacat tatttgaatt tcctcgacta cgagaccgcg
    56821 gaaacaaaac gatgaaatat atcggtgaaa ttactgtgaa tggttttcca ggtatcgaaa
    56881 gtggcgaaaa accattttat gaagaaccac gtatgcctaa agatctaatc acaagaactg
    56941 attacgtaac ggctaagaat gtattagatg cgaagggtgc cgatgctttg gtcgaatgga
    57001 tcaaaggaca ggaaaatttg ttgttgacgg atacgacatt tagagacgcc catcaaagtc
    57061 tgctagcaac tcgtgttcgg acaaaggatt tcaagcagat tgcacgtttg actggagaag
    57121 gtcttcctga actcttttca agtgagatgt gggggggagc aacatttgat gtggcttacc
    57181 gctttttgaa tgaagatcct tggcaacggt tgagaaaaat tcgttcttta atgcctaata
    57241 cgttgctgca aatgcttttc agaggttcaa atgccgttgg gtattctaat tatcctgaca
    57301 atgtattggt ggaatttgtc aaagaagcag cagcgcaagg aatcgatgta tttcgaatct
    57361 ttgatagttt gaactggact ccacaaatgg aaaaaagtat ccaagctgta cgggatactg
    57421 gtaaaatcgc agaggcagct atttgctata ctggcgatat caacgatcca agtcgggcaa
    57481 aatacaatgt tcagtattat aaagacatgg caaaggaact agaacaactg ggtgcgcata
    57541 tcattgcaat caaagatatg gctgggctgc ttaaaccgca ggcagcttat cgtttgatta
    57601 gtgaattgaa agaaacgaca gatctgccaa tccatctgca tactcatgat accagcggga
    57661 atggaatcat cacttattct gctgcttcaa aagcaggagt agacatcgtt gacgtggcaa
    57721 tgagtgcaat gagtgggaat actagtcagc ctagcatgag cagcttgtat tatgcactgg
    57781 tcaatggtcc acggctgcca gaaatcacga ttgaaaatgc acagaaatta aaccattatt
    57841 gggaagatgt gcgcatgtat tacaaaccgt ttgaaaacgg cttgaatgct cctgaaacag
    57901 aagtatatat gcatgaaatg ccaggaggac agtattcgaa tctacagcaa caggcaaaag
    57961 ctgtcggatt aggtcatcgc tgggatgaaa tcaaacaaat gtatcatact gtcaacttga
    58021 tgtttggcga tatcgttaaa gtaactccat catctaaagt agttggagat atggcactat
    58081 ttatggtcca aaatgatctt acagaagaag atatttatga aaaaggcgaa acactaagtt
    58141 tccctgaatc tgttgtgact ttcttccaag gagaactagg gcaacccgtt ggcggatttc
    58201 ctaaaaaatt gcaaaagatt attcttaaag gcagacctgc attgaatgaa cgtccaggtc
    58261 ttttggctga atcagtcgac ttcaacgaag taaaaaaaga attggcagaa aaaattggct
    58321 atgaaccaaa acaagacgaa gtattaagct atttgatgta tccgcaagtc tttttggact
    58381 atcaaaaagc atataatcaa tttggcgatg ttacattact ggacacacca acatttttcc
    58441 aaggtatacg tttgggtgaa acaatcaatg tacaaatcga acgcggaaaa acgcttatca
    58501 ttcgtttaga tgaaatcgga gaacccgata tcgaagggaa tcgcgtactg ttcttcaatt
    58561 tgaatggaca aagacgtgaa atcactatca aagataattc gatcatcagt acggtccaaa
    58621 cccgattgaa agctgaaccg acaaaccgag aacaaattgg agcaacgatg tctggttctg
    58681 ttttggatgt attagtgaaa aaaggcgata acgttaaaaa aggtgatacg ttgatgatta
    58741 ctgaagcgat gaaaatggaa acggccatcg aagcacgttt tgacggagaa gtcgcacatg
    58801 tatatgtgtc ttcaggagac actatttcgt ctggtgacct tttgattgaa gtgactgaga
    58861 aataataagc gaaaggtggg gaaacatgaa aagactgggt ttgttttggc tgattctgct
    58921 attagttatt gtggcattct acatggaacc agtcttgttt ccgccaaaac ttgaagtgaa
    58981 ggaaaactct gatcaacaaa tatcaaacca aatgacaacg tggaaatacc aggagcttaa
    59041 agcaactggt ttttctactt atatcggaaa accgatcgat cagctggaaa aggattttgg
    59101 ccaagcctat gatcgattaa ctagcggatt tggttttgaa acaagatatt atgaaaataa
    59161 agctagtcat ctcacatttg aagcgaacat ccaagacagc agagtcacaa ctgttaaagt
    59221 actgcaaaca gggcctgctg atatcgcgcc gttttattta gggatgacga tgcaagatct
    59281 tacgaaaatc actactatct atactaattt cacttttgag taccatgata cagaagttgg
    59341 aatcgaacta atggaagagg atatgaatta tcgtccgcta attgcttttg ataacgacac
    59401 ttttgctatt ttattttttg atcaaacaac aaacggccta ttttcagttg tgtatttaga
    59461 taaagatagt cttttgaaac tattgccata tcaagtgttt ggtgaaggcc ttccacatta
    59521 tcaagcagat aaatcggctg attgggaatc aatcaatcgc gttaaagaac gaaaaagtac
    59581 gactttatta aatatgctta gaagagaaga cgaacttccc atatacaatc aaacacttgc
    59641 ctttcaaaac cacagtcagc tgctactaca tgattttttg acaaggcctg aggaaatatt
    59701 gtcagaggaa cgtatggctg attggcaaaa atcgctaggc agtgctcaaa cgacagtgaa
    59761 attctcactg acgaatgatg aattggagaa gttaatcagt aaacaaaaat tagaaaatac
    59821 tagtgggcta tttattcatc cggcgattga cgctactttt tcgtttttat attggtacag
    59881 cgatccttac aaccatgacc gtttcatgga cccgggaacg gattctgtag gcattgcttt
    59941 ttctaaagaa aatatgttag tcttattgca agaagaaaac ggagaaagta gtgatagcag
    60001 tgatcgttaa cgaagagctg tttcaactgg aggatcaatg cctccaagtc gctgaaatga
    60061 taaaaaagag taaaacgatg gcttcttatc ttgaacataa acagtcaatg gaagaatctg
    60121 caaaagtttg ccagttgaaa gaagcgttct tgtctaagaa agaagcattt gaaaatgtag
    60181 cggcatatgg tgagtatgca cctgattatc gtgaaaaaaa acgagctgtt cgaaaagcaa
    60241 aaagacagct tgatatggat gagcatgttg cagctttccg atatagtgaa acacagctgc
    60301 aaagtctgtt ggatcaaatt gtccaagtac ttgccgaaga agtttctaat gaaatcaaag
    60361 tagatgcagg tagcccgttt tttgaaacga aaaaacactt tggatgcgga ggtaattgtc
    60421 atgcaattag acaatagtga atttgaaata acaaaacgac gcgggctgat cgtatgggtc
    60481 tacagcttaa aacaattaaa gaatctgaaa aagttcggct tgttgcatta tgtctcaaga
    60541 agaatgaaat atgtcgtgct ttacttaaat gaagagacat tggaagaaac agaagaacga
    60601 atccgtaaat tacattttgt tcgaaacgta gaacgttctt accgaccgga tatcgaaatg
    60661 aattttgccg aaaaaatagg aaacaaaaca gtattaaaag acgaaggttt cgaaattgaa
    60721 gagctaaata cagaaatccg attagctgaa catctttaaa aatctcttat gagcagtttt
    60781 taaataaaaa ataacttgga taacgatcga tttttgatca ttgtccaagt atttttttgg
    60841 tatactagta ccagtaccat ttctaaggag tgagtagatg cggataatat caggaaatta
    60901 tggcgggcgc cgtttaaaaa gtttagcagg ggcgaacacc agaccaacta cagataaagt
    60961 aaaagaatcc atattcaata tgattggacc atattttgat ggagagacag tattggatct
    61021 gtttgcagga agcggtggtt tagctatcga agcagtatct agaggctgca gtcatgcggt
    61081 atgcgtagac aaaaattacc aagcgctaaa aatcatcaaa gaaaacatcg aaattacgaa
    61141 agaaccagag aaatttacca caataaaact tgatgcggac aaagcaatcg aacggctagc
    61201 agcggagaaa caacaattcg actacttgtt tcttgatccg ccatatgcaa aacaaaaaat
    61261 aatcgatcaa atccataaaa tggaagaact gggacttttt tctaaaaccg cagttatcgt
    61321 atgcgaaacg gataaaacag tcgaattgcc agaaactgtg gctggattcc atcaaataag
    61381 aaaacagatt tatggcataa cggccgtaac tatctataga aaagagggaa atcaatgaaa
    61441 cgtgcattat tcccaggaag ttttgatcct tttacaaaag gtcacttaga tacggtggaa
    61501 cgtgcggcta aattattcga tgaggtagta attggtgttt ttatcaatac ttcaaagaaa
    61561 agtttgtttc ccccagaaga aagaatgaca ttgatcacaa aagctgtttc tcatcttcct
    61621 aatgtaaaag tcatgcatca agaaaatcaa ttgacggtag aaacagcaaa agagattggt
    61681 gcagatgcgt tgatcagagg catacgaagt atcaaagact ttgaatatga acgagagatt
    61741 gctcagatga atcatcatct atatgatgaa ttggaaactg tcttcttatt agcaaaacca
    61801 gaatatagtc acgtgagttc cagtatttta aaagaagtac tgcactttgg cggagatgtg
    61861 agcagctact taccgccagt gatcaacgaa gctttagctg gaaagcgtga atcaaatgaa
    61921 agctaaacaa tggttgaaaa cagtactgat tatcatattt ggaatgttga tcattggatt
    61981 tgtagtagtt cccttgccgt attatgtaga atcaccggga gccacgatcg atctgaaaga
    62041 actaattacg gtcaatgata aggaagataa aggttctggg tctttctctc ttacctctgt
    62101 tggtatcagg caagctacag gtttcttagc tttaaaagct aaaatgaccc cttttgaaga
    62161 attgatcagt gaagaagaat tgactggtgg ggcgacaaat gaagaatata atcaaatgca
    62221 acagtattat atggattctt cccaaaatgc agcaatcgaa caagcgttaa aacttgcaga
    62281 caagccttat aaaatggaat tcaaaggcgt atatgtcatg agtgtagaga aaacatcgaa
    62341 tttctttggc aagctttcag ttggagatac agtaacaaaa gtagacggga aaagctttca
    62401 aagtacagaa gcatttatgg attatgtaaa aagccaaaaa gttggacaaa caatcgaaat
    62461 cagttatttg cataatggtg aagaaaaaac agcttctggt gatctgatcg agttaccaac
    62521 agataaaaag gcaggaatag gtatcacgct tactgaccat accgagatcg aatcagatac
    62581 agatgttcgt attaattctg gctctatcgg tgggccatca gcgggattga tgttcacttt
    62641 agaaatctat gaacaagtca ctggtaaaaa cttgcgacat ggtaagcaaa ttgcaggaac
    62701 tggaacgatc aacagtgaag gagaagttgg ccgaatcgga ggaatcgata aaaaagtggc
    62761 aagtgcggat aaagctggcg tagagatttt ctttgcacca gatgatacga tttcagacga
    62821 tgttaagaaa gaatatcctg aaataaaaac caactacgaa gaagcaaaag ctgccgctaa
    62881 aaaattgaat tcctcaatga aaatcgtacc agtaaaaaca gtacaagaag cgcttgacta
    62941 tctagagaaa atgaaatgaa actaaataat taaagagggg ttgtgacacg agttctgtca
    63001 caacccctct ttttcgtcaa aaatcttcac gataaaaata aaaacaaaag aaaaaattga
    63061 cgtaattaga tgttgaaggg acgattttgc cagagctcac tgtttcttca attttaaatg
    63121 aaaggagtaa tgaatgattt ttactggtca ttctttgaaa aaagaacaat gatagaaaaa
    63181 atcagcaaga aaatactagc ttcacccaaa ttgatcattt ggatgtctat gatagttctt
    63241 atcttttgta tcgcaagtgg ttttttatta tcaaatttgc tattttatag agaaacagaa
    63301 gagactgctg caaaagaaat actttcttcg tttagttcca gtggagaaga tccatcagct
    63361 gaaggaaacg atgtagaaga ggatgctcgt ccaaaggtca tgtacaccga tatcaaagga
    63421 agtgtgaagg agcctggtat ctattccttt tcttcagaag aacgggtcta tgatgtacta
    63481 aagcgtgcag gtggattatt agaagaagcg gatagcgatc gaatcaactt ttctgcgaag
    63541 atagaagatc agcaagtcct atatatccct gctgtaggtg aagagctgcc agaacatctt
    63601 gatcagttgg catcctcgga aggaaaacag cctacagcag attccgagcc tcccaaaata
    63661 aatatcaata cagccactcc atcagaactt cagcagattc ctgggatcgg atcagtcaaa
    63721 gcgcaagaaa tcattcgatt tagagaagaa aacggatcct tccagaaagt ggaagacctg
    63781 caggagattt ctggtatcgg tgaaaagaca gttgaaaaac taaaaaactt cgttacaatt
    63841 aaatagaatg attcaaaatc gactggcacg agcaataaat ggacgctaat ggatactaag
    63901 aataatccaa ttctaagagc ttggcaggac gaggaggtag tatttttgga taggaaatag
    63961 cttgattgtt cgcaaagaaa cattaaaaaa accaatgctg aatgtctatc tccaaaataa
    64021 aatatctggg attcatatta tgaatacggc tgtcagcggc aataacagtc aagctctacg
    64081 agaacgcttt gccaaagatg tacttagcta tacagcagat aaagtattta ttctcattgg
    64141 gacgaatgat ctagcagagc ataaacaact ttctaaagaa acgtaccaaa aaatctgcag
    64201 tggatgatag ttcagctgaa aaaagactat agttctgatc atgctttctt tatcacgcca
    64261 ccagcagtcg atgagacaaa acaaaaatat cggaataaca agttgatcaa agaatatatc
    64321 tctgttctca cagaagtagc aaaacgccat gattgtgaag tatcgatttt tatacggaat
    64381 ttcttgaaca aatggattcg aagaattttt ataatgggct aatggatgac ggacttcatt
    64441 tcggcagtga agggtaggat cttttgtcag acaggatgat tcgttatatt taaagaatat
    64501 tttgtttaag caatgagtac tttttaatca acttgaaaaa acggaggaat ataaaacatg
    64561 ttagaaaaat cttcaaaagc atgcgatacg aataaagaaa aagttactga taagtcacat
    64621 aatgaacgca ttccttggga ccagtacttt atggctcagg cagtactgtt atctttacgt
    64681 agtacgtgta cacgtttaga agtaggagcg acactcgttc gagaaaaaag gatcattgct
    64741 ggcggctata atggagcagt cagtggagat gtccactgca tagatgaagg gtgttacgtc
    64801 gtggatggac actgcttgcg aacgatccat gcagagatga atgcactcct acagtgtgca
    64861 aaacttggta tcccgacgga aggttcagaa atctatgtca cacattttcc atgtttggcc
    64921 tgtacaaagg ctttgttgca agctggtgtg aaagggatca attatctcca tgactatcga
    64981 aatgatcctt atgcccaagc gttgatcgaa caattaggtg tatctgtaca ccaagttgct
    65041 ttagactcac agcatttttc aaaacttcaa aatgaattaa caggaaaaca tgtctagcgt
    65101 tttagaaagg catgttcgaa atcggttgat ttttccggca attacgtcga tgattatctg
    65161 tttgttgtat tacactgaca attggggtta tcgtttgctt cttgccggaa tttttttatg
    65221 gttttcgcta aaagaaggac gttttatcgc gtacttgtgt attttgtgta gcataacggt
    65281 atccatttct tataggctac aagagtattc atttttagag gaaaaaacaa catctgcgca
    65341 aattactgta tttacagata cgattcgtgc aaatggtaat tttttgacaa tggaaggaag
    65401 gttatcagag aataaacaaa agctccagct ttcttatcaa gtaaacacag aagcagaatt
    65461 gaaaacatgg cttaacttcc gaggaaaggg aatagaattg actgctgaag gcgagttcat
    65521 ctctccagca aaaaagcgga atctttataa ttttgaccaa gaagcttact tcaggaacca
    65581 tagaatttct ggacggttcc aaattcaaaa gattgaacag atcaccatgc aaaatcactg
    65641 gcgaaactgg cttcagagaa aaagaggatc ttttatctcc tttgtccaat ctgaatttcc
    65701 gcaaaaaaca agtgtatata ttaattcttt attatggggc tataaagatg caggattcag
    65761 agaaccagaa gaaattttta gggagagtgg tttgctgcat ctgttcagtc tatcgggtct
    65821 acacatccaa ttttatttag ggtggctgta ttatttattt cgacgtatcg gatggccact
    65881 acacgtcagt attattccgt tgagtctact aactatctgt tatgtttcta ttgcaggaag
    65941 ttctatttct gttttgcgtg ctagtttgct gtttttattg cgattgctag tcaaattgct
    66001 gaataagcat tattccacca tggacttatt tgcagttgtc ttttggcttc ttttactatt
    66061 tgatcctcga tttctttttc atgcgggtgg acagcttagt tttttcatga gttttttgtt
    66121 tatcctgatt tcttttgatg gagattggtt gaaaaaaacg gcctctcacg tcctgtttac
    66181 tatgatcacg atgccgttga ttgtatggca tttttatgag tggcctattt taggtagtct
    66241 ttttacatta ctgattgcac cggtattcaa gtttcttttt cttcctggtg tgttatttct
    66301 cacactcttc aatcgctggc tgccggaaac tttacttttg ctgatagaag agggattaat
    66361 cttatttgag cgaataatcg atttttcttc agggtcgtct ctcattatag gacgtttgcc
    66421 ccttgtgcta tctgggatgt atattttcgg attactgatt tgtatggata ggttgaaaga
    66481 acaacctgtg aaacacttgt tttatgttgg tcttttttct tttatttttt tgactttgcc
    66541 atttttacgc ttttcttcaa cgatcgcttt tatcgatgtc ggtcaaggag acagtatctt
    66601 tttgcaaagt ccttttcgaa aagaaacgat tttgatcgat actggaggtc agttacactt
    66661 tgaaagagaa aagtgggcgc aaggtatgat acgtcctcct gctgaaagaa atatcgttcc
    66721 atatctaaaa ggtcagggga taagtgtaat cgataaactc atcttgacac acgacgatac
    66781 ggatcatgtg ggagaattac ccacgttatc tcagcatttt actatcaaaa aaatttatat
    66841 cggttggggc gctggacgaa atgagccatt acaaaggcat ttatctgaag ctcaaaaaaa
    66901 tggtactgag atcattgaaa tcaaacatgg ggacaggatc agcggttatt atgatcttta
    66961 tgtattgact ccttttgaaa agggagaggg aagaaatgaa gattcgattg gtctatggat
    67021 ggaatataac aataggcgct ttttattttt aggagatttg aatcaagcaa tggaaaaaca
    67081 attgcttttg atttatccta atctgaaagc cgatgtagtc aaattaggtc atcacggtag
    67141 ccgtacgtca agtaacacag attttctttc ccatattgaa gcaaaacatg ggattatatc
    67201 ctgcggcgta aataatcgtt atggtcaccc gcacagcgaa gtattggaga cactagaaga
    67261 aaatcagata aaaacatggc gaacggatca acaaggaatg atcacctatc gctggcatcc
    67321 agtttttcat cctcatgggc tagtggaaac aatgatagac tagtaattct catctgctca
    67381 tggtaggata atttttgaaa gtggtgagta atttgagtat acaagcagag ttacaaaaaa
    67441 tacgtaaaga aaaacttgca tcttgttact tgattttagg aacagaaaag tttctccaag
    67501 atcaagttcg gacagaaatt gtcaaaaaaa tccaattaga gggcgaagat gacttaaatt
    67561 ttttaagttt tgacatggaa aatgcaagtt tagatgaggt agtagctgaa gcagagacgt
    67621 tgccgttttt cggagatcaa cggttagtat tcgtagagaa tccttatttt ttaacaggtg
    67681 aaaaagtaaa caatggaatc gaacaaaata ctgatttatt gacggcttat ttgaaagaac
    67741 cattagaatc gactgtatta gtattctttg cgccatatga aaagttggat gaacggaaaa
    67801 aagtaacgaa acaattgaaa aaaacagcaa ttatcatcaa tgtccaacag ttgaatgaaa
    67861 aagaagtacg tcaatatctg atgaatacct tagagaattc ctcaatcaaa atggaacgaa
    67921 gtgcaattga tttattcttg aggttgaccg acttagattt atccaaaatg atgagagagt
    67981 tacagaaatt ggtcctttat ggacaaaatc aacagcaaat cacggtaaga gaagtagaag
    68041 aattagttcc taaaacgtta gaacataata tttttgacat gacccaatat attttaaaag
    68101 ggaagacgga acaagctttg cgtttatacg aagatttagt tatgcaaggg gaagaaacaa
    68161 tcaaaattaa tgcgattttg ctttctcaat tacgtttatt tttacagaca aaatttcttg
    68221 taaagattgg ttatcagcag gcaaatattg ctgaaacatt gaaaatccat ccatatcgtg
    68281 taaaacttgc catgcaggaa gtaaaaaaat tcgatgaaca tttgctggta cagttgtttg
    68341 atcaattagt tgagatggat tatcaaatca aaacgggaca aatcgaaaaa gaattaagtt
    68401 ttcaactatt cgttttgcgc acagggcagt tgttaagaaa tcgataagaa gaaagacgag
    68461 ggcaaagcat gcgggaaggt cgtttgtttt tgtccttttt ttgacaaata aaaaaatcgg
    68521 ccgaagccga ttcttttatt tagcgatttt tttgcttaaa cgagatttgt cgcggttagc
    68581 tttgtttttg tggattagac cttttgattc agccatatca atagctttaa cagcttcttt
    68641 atataacgca tctacgttgt cagcacctga agctacagca tcttcaaatt ttttgattgc
    68701 agtacgcata gcgcttgttt gagatgaatt tttcacatta gcgttttcgc ttgtacgaac
    68761 acgtttgatt gcagattcaa tgtttggcat tagtttcacc tccgataact taagttctta
    68821 cgcgtgttac cgcataattc aacattcatt attataccta aaggcagcgt gctttgcaat
    68881 atgaacatga caagtttttt tccttaacag tcttttatta ggattttttt tcagtaaaaa
    68941 cggatagtac aagctgaaaa aaatcaaaaa gacgctattg tgggaggcaa tagcgtcaaa
    69001 ccaaactaga tttttattta tctatttaaa caaagtttga atgggaatgt ttggtactaa
    69061 gttattatat aacaaagaga accgtttgca aagttttttt tgaaaaggga tacgattccc
    69121 tgttgttctt tgtttttcta ttaagaaact gcagttattt tataaccagt atcctttgat
    69181 cagtaaaggc tattctggtg taccgattat tttaatgtat tcatcgatat tttttatcaa
    69241 catttttttt atgaccaact ggttccgacg gttccatttg ccacttactt ccagcacata
    69301 tttatttagg ggcaaacgta agatatattt tgccaaatag cgatctttaa gcaggccatt
    69361 gatcacacgg tcctcaagct gcaagttgat cccagtcagc atcattggct gttcttgtaa
    69421 aacgtacgta tcaattaaaa gaccttcaaa agtttccaca agcgtcttcc tttcttttca
    69481 aagtaacctg aaaacaggca ctaattcttc cattttgata ttcgttgaag tggatgcgat
    69541 cattctttct tttgtccact gtaatctagt ataacaaaaa agagcagaag agccaaaaag
    69601 aaaaaagagc atcgaattct ataggaagtc tcattggcac ggcctaaatt attacaaaac
    69661 attgattaaa aaaagcccct gcatcgcagg agcatcccct atatcgtttt aataggtcaa
    69721 tcaaaaagca gtatcaaaca cattttgtat caaatcagga aataatagat aagctaagcc
    69781 cagaatcaaa agtaccgcta gaagaaacca gccgattgtt cctatgaaat actttaattt
    69841 ttcgaacatt gtatcacctc gttgtcagta tatcacggat cagtaaattg ggggtgttaa
    69901 gaatctccta aaatagacaa aatttgttct attttcctaa agcaaatact tgtaagatac
    69961 agtaagtgat accagaagca atgaccggtc cggcggcgat tcctcttaag aagacaacac
    70021 ctaaaatcgt gccgaaaaca agtgcaacag tgatttgtgg atcagaagca atcaatccta
    70081 cacccttgga agataagaca gccactagcc caccgcaaaa aacagctacc cagccaatag
    70141 gggatttaaa ggcttctaat agatcacgta gaccgatttc tcctgttgcg atagggatta
    70201 gaatagccgc tgtgatgatc gtcaccccaa tattgattcc tttgccttga agccaaggga
    70261 aaaaggaacg ggaggctggt atgagcttca taatcaaaac aaaagctgcc gctatcatca
    70321 gactttgatt tttagctaaa attgctacaa gtaaaataat tcctaaaaaa agccaacttt
    70381 ccatgaaata tattcctcac tttcttcgaa acaatcataa catgttttta gaaagttaca
    70441 aaagtaggaa ttcatccttc aagacctatt cataagctgt ttttttcata aaataaacaa
    70501 aatcataaaa aaacactttg cttatttgac agaagacagc cgcgctttgt atagtggaca
    70561 agacttacat ttttttgaag aatcagaaag atcaggtaaa ggtcttagta aacgcaagcc
    70621 attcagaata accaatattg tgcttccttc gtgtcctaca actccaagag gtagatttaa
    70681 aaattgcagg aagttagaag caatcaacaa gagaataaca agaatcgaaa aaataatatt
    70741 ttgtttgatg atccgccgca tttttaaaga tagccgatga ctgtagacaa atttatttaa
    70801 gtcgcttttc attaagacga tgtctgcgat atccatagcg gcatctgtcc cttgccccat
    70861 agcgacacct aaagaagcgg ctgctaaagc aggcgcatca ttgactccat caccaaccat
    70921 cgcatttact tggtaaagtt cattctcttt tttgactagt tgtgtttttt cttccggagt
    70981 acagcttgca tagtaatgtt caatccctac ttgttcagca atggcctttg ctgtgccaga
    71041 atggtcgccg gttatcatac ttgtatggat gttttgggat ttaaagtagg aaattacttg
    71101 ttgggtattt gcttttggaa tatctagcag acctaaaaca gcaattaact gctgatcttt
    71161 agaaagataa atcacggttt tgccttgatt ttccaaacgt tcgatcattt ccgcaatctc
    71221 tttgctgatt atcatcgtct caggatcata tgcgtgcttg ccaactttcc attgcgcacc
    71281 taaataggta gtttgtaatc caaaaccagt aatttcttct attggtaagt attggatttc
    71341 ttcaggtatt gtgcaatcga aatattgcaa aatcgcatga gcaagtggat gcgtcgtttt
    71401 ttgttccata gcaacaagaa gttgttgtgc caaatccttt tcatcaaaga aaaagctgtc
    71461 agttacaact ggttttcctt tagtgagcgt tcctgtttta tcaaaggcaa tcgcttttaa
    71521 ttcagaaagc ttttctaaat ggataccgcc tttgattaaa atcccatttt ttgcagaatt
    71581 tgaaagagca gctagagaag ctggtgtagc agatgcgaca agcgcacaag gagaagccac
    71641 aactaataat accattcctc gataaaagct ttcatcccaa ctccaaccta ggaaaaagtg
    71701 ggggagaagg atcattagag gaacagcaag caaaatcaat tttacataat tattttcgat
    71761 tcgttcgata aatgaagctg tttgagttgg cgtattctgc gcttcttcta ctaattggat
    71821 aatcttagca aagacagttt cggaactcgt ttttgttact cgaatcgtga tgggatttcc
    71881 ttgattcagc gttcctgcat acatctcatc gcctaaatat ttttctacag gcacagattc
    71941 accgcttaat gcagactcat cgatcgttgc ctgtgtgctt tctaaaaagc catctatcgg
    72001 aacagctgct cctttagcga caaataccaa gttatccacc tttagttgag aaacatctac
    72061 ttcttgcatt tgtccatttt cttttaataa ttgggctttt tgtggttgaa gattcatcaa
    72121 agcagtaatt tctttttgac ttttattagt tgtgtattcc tctaatgcac cgcttaaaca
    72181 aaagatgaat gtcaacattg ctccttcaaa gtaattaccg atcaaacagg caccaatagc
    72241 agctaaagcc atcaataaat ccacattcag tgtccgatca ttccacaatt ctttcaagcc
    72301 ttccgatgtt tgtttatatc caccgatgat aatggcactg atataaaaaa gtggggtgat
    72361 ggtaagagag aatcggtcga agataaaacc gaagatcata aataaaaggc agccgattgt
    72421 tgctgcaaaa gcttttttct gattagtcat cgttattaga cctccttgtc aaaacaagga
    72481 tagcataata aaaatgtaat tgataataat aacagctaaa tgagaacgat aatggttatc
    72541 atttggtgat taagcttgat gttgttttta ttatttataa tgattattat taaagaaaat
    72601 aaaaaaccag aacatttctc aaataagag
//
LOCUS       DAJSBL010000006        71080 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.006, whole genome shotgun sequence.
ACCESSION   DAJSBL010000006 DAJSBL010000000
VERSION     DAJSBL010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 71080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 71080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..71080
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.006"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            161..391
                     /locus_tag="ONT85_000506"
     CDS             161..391
                     /locus_tag="ONT85_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HCR2377505.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            488..1789
                     /gene="murA"
                     /locus_tag="ONT85_000507"
     CDS             488..1789
                     /gene="murA"
                     /locus_tag="ONT85_000507"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HCR2377506.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            1802..1978
                     /locus_tag="ONT85_000508"
     CDS             1802..1978
                     /locus_tag="ONT85_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HCR2377507.1"
                     /translation="MSNQRYILLTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            2113..2409
                     /gene="yidD"
                     /locus_tag="ONT85_000509"
     CDS             2113..2409
                     /gene="yidD"
                     /locus_tag="ONT85_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HCR2377508.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFQLTRNKKDKKEPDYKKVVKNTSSTER"
     gene            2593..3060
                     /locus_tag="ONT85_000510"
     CDS             2593..3060
                     /locus_tag="ONT85_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR2377509.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVAPTYQGKGIGGKLLETAFQKLSQLASSQTQIIIRTGSTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            3145..4029
                     /locus_tag="ONT85_000511"
     CDS             3145..4029
                     /locus_tag="ONT85_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HCR2377510.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKAQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLN
                     GDKTFSKLGTAVQMAWRYCERKGLDKNSYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            4143..4379
                     /locus_tag="ONT85_000512"
     CDS             4143..4379
                     /locus_tag="ONT85_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HCR2377511.1"
                     /translation="MMSKKNKDVEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYG
                     PKEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            4467..4547
                     /locus_tag="ONT85_000513"
     tRNA            4467..4547
                     /locus_tag="ONT85_000513"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4501..4503,aa:Tyr,seq:gta)
     gene            4554..4625
                     /locus_tag="ONT85_000514"
     tRNA            4554..4625
                     /locus_tag="ONT85_000514"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4586..4588,aa:Gln,seq:ttg)
     gene            4750..4920
                     /locus_tag="ONT85_000515"
     CDS             4750..4920
                     /locus_tag="ONT85_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2377512.1"
                     /translation="MKWKEKVDEAAEKLYDLVKSEKYNIEVNIPKKGGKAVQVKSKRP
                     TNHTKKWMAKNR"
     gene            5140..5847
                     /locus_tag="ONT85_000516"
     CDS             5140..5847
                     /locus_tag="ONT85_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR2377513.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(5907..6551)
                     /locus_tag="ONT85_000517"
     CDS             complement(5907..6551)
                     /locus_tag="ONT85_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HCR2377514.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGPVSYHEQLVTDHI"
     gene            6678..6965
                     /locus_tag="ONT85_000518"
     CDS             6678..6965
                     /locus_tag="ONT85_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2377515.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            7073..7393
                     /locus_tag="ONT85_000519"
     CDS             7073..7393
                     /locus_tag="ONT85_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCR2377516.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            7474..8787
                     /locus_tag="ONT85_000520"
     CDS             7474..8787
                     /locus_tag="ONT85_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HCR2377517.1"
                     /translation="MNHFWGRRLLTRDIKAIDCEEVNLPNYEKIAAVERLGNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEIAKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKML"
     gene            8784..9503
                     /locus_tag="ONT85_000521"
     CDS             8784..9503
                     /locus_tag="ONT85_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HCR2377518.1"
                     /translation="MRCSCCSKDIVQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVKSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQVAQPKIIRTISLAR"
     gene            9631..10182
                     /gene="raiA"
                     /locus_tag="ONT85_000522"
     CDS             9631..10182
                     /gene="raiA"
                     /locus_tag="ONT85_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HCR2377519.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADATALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            10404..12938
                     /gene="secA"
                     /locus_tag="ONT85_000523"
     CDS             10404..12938
                     /gene="secA"
                     /locus_tag="ONT85_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HCR2377520.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPHHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKSFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            13064..14165
                     /gene="prfB"
                     /locus_tag="ONT85_000524"
     CDS             join(13064..13135,13137..14165)
                     /gene="prfB"
                     /locus_tag="ONT85_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139124933.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HCR2377521.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRRSLDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            14270..14956
                     /gene="ftsE"
                     /locus_tag="ONT85_000525"
     CDS             14270..14956
                     /gene="ftsE"
                     /locus_tag="ONT85_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HCR2377522.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            14940..15833
                     /locus_tag="ONT85_000526"
     CDS             14940..15833
                     /locus_tag="ONT85_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR2377523.1"
                     /translation="MDTMIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFM
                     AVIMNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELK
                     KIQNEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSD
                     RIFQIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRW
                     PFFLEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMV
                     GIGVIIGSLGSIISMRRFLKF"
     gene            15985..17433
                     /gene="cls"
                     /locus_tag="ONT85_000527"
     CDS             15985..17433
                     /gene="cls"
                     /locus_tag="ONT85_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HCR2377524.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWVLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHSTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
     gene            17770..18627
                     /locus_tag="ONT85_000528"
     CDS             17770..18627
                     /locus_tag="ONT85_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HCR2377525.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            18742..19662
                     /gene="pstC"
                     /locus_tag="ONT85_000529"
     CDS             18742..19662
                     /gene="pstC"
                     /locus_tag="ONT85_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HCR2377526.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            19662..20546
                     /gene="pstA"
                     /locus_tag="ONT85_000530"
     CDS             19662..20546
                     /gene="pstA"
                     /locus_tag="ONT85_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HCR2377527.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            20561..21367
                     /gene="pstB"
                     /locus_tag="ONT85_000531"
     CDS             20561..21367
                     /gene="pstB"
                     /locus_tag="ONT85_000531"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002408906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HCR2377528.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            21383..22141
                     /locus_tag="ONT85_000532"
     CDS             21383..22141
                     /locus_tag="ONT85_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HCR2377529.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            22154..22831
                     /gene="phoU"
                     /locus_tag="ONT85_000533"
     CDS             22154..22831
                     /gene="phoU"
                     /locus_tag="ONT85_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HCR2377530.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            23069..24100
                     /locus_tag="ONT85_000534"
     CDS             23069..24100
                     /locus_tag="ONT85_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HCR2377531.1"
                     /translation="MQKKISIITIFFICVLTSWVIQHQAALYEVPVIEIEKVRIKEDH
                     QLITGKLMNTDQKGESIYLSVPYQKNQIETANIKARQMYLVQMHGDEWQLVAKKNDGW
                     LFFFTSIFIGAMLLIGGKQGLFALGSLIINIVLLLLLLLIFTKTEGISLLNISILFVV
                     IGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYLTRP
                     YRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVIGSM
                     TNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPISVLF
                     FLTVRRLKR"
     gene            24097..24864
                     /locus_tag="ONT85_000535"
     CDS             24097..24864
                     /locus_tag="ONT85_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HCR2377532.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            24964..26532
                     /gene="liaX"
                     /locus_tag="ONT85_000536"
     CDS             24964..26532
                     /gene="liaX"
                     /locus_tag="ONT85_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HCR2377533.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEIAQVQL
                     STTTGSIYLKDTDK"
     gene            26829..27149
                     /locus_tag="ONT85_000537"
     CDS             26829..27149
                     /locus_tag="ONT85_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HCR2377534.1"
                     /translation="MMKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVA
                     FGSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDE
                     WSDF"
     gene            27149..27499
                     /locus_tag="ONT85_000538"
     CDS             27149..27499
                     /locus_tag="ONT85_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HCR2377535.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            27666..28604
                     /locus_tag="ONT85_000539"
     CDS             27666..28604
                     /locus_tag="ONT85_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HCR2377536.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            28619..29449
                     /locus_tag="ONT85_000540"
     CDS             28619..29449
                     /locus_tag="ONT85_000540"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HCR2377537.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            29493..30518
                     /locus_tag="ONT85_000541"
     CDS             29493..30518
                     /locus_tag="ONT85_000541"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HCR2377538.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGIGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQMMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            30533..31471
                     /gene="galU"
                     /locus_tag="ONT85_000542"
     CDS             30533..31471
                     /gene="galU"
                     /locus_tag="ONT85_000542"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HCR2377539.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYILSLGKTLEAEGSKIDHNPKGITEIKEEKKK"
     gene            31596..32036
                     /locus_tag="ONT85_000543"
     CDS             31596..32036
                     /locus_tag="ONT85_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HCR2377540.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTKDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIATKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            32039..32641
                     /locus_tag="ONT85_000544"
     CDS             32039..32641
                     /locus_tag="ONT85_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HCR2377541.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSTELTGIAKEKAQDLSQQAGDLAGSVKQKVSESADEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDMKKPADELGSIAKDTSEDIMIDVKDAAGKAGDTVNEG
                     MSEAKPVADDSKEMMKMAKEDAKEAKKEIKDTMTDSSTNH"
     gene            complement(32700..33806)
                     /locus_tag="ONT85_000545"
     CDS             complement(32700..33806)
                     /locus_tag="ONT85_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HCR2377542.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVERFDAISDILTGTDSYTDLTSIIQRLQLIKTLEEKDKLIEAGKWADVAF
                     EIGFKAVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGNTKISP
                     NELVLFDLGVVWNGYCSDATRTIAYQKPTDFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLEK"
     gene            34016..35017
                     /gene="ccpA"
                     /locus_tag="ONT85_000546"
     CDS             34016..35017
                     /gene="ccpA"
                     /locus_tag="ONT85_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HCR2377543.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            complement(35354..37699)
                     /locus_tag="ONT85_000547"
     CDS             complement(35354..37699)
                     /locus_tag="ONT85_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HCR2377544.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLMFAA
                     LGIGIGAGYFAYLVEDTQLPTKKALQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIILSAQVEKYFSKDEIIATYLNVSPFGRNNKGQNIAGVQEAAK
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSDAYYQKAQQTIQNKGYTIHSTIDKDVYAALQNGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLIGSES
                     RVSDYATTWQEGENAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINAKITTPFKDAISSLNGNLGKA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSKARIRSANYLANLINQAY
                     QAKPDIFGTDEKFELSSDVMKKKVSGFTGQTPGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANQATKESDEKDD"
     gene            37978..38880
                     /locus_tag="ONT85_000548"
     CDS             37978..38880
                     /locus_tag="ONT85_000548"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HCR2377545.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAYMAEKGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGTEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKIIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     IAMFKAQTFDPEVFPELLAEEVIL"
     gene            39093..40868
                     /locus_tag="ONT85_000549"
     CDS             39093..40868
                     /locus_tag="ONT85_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HCR2377546.1"
                     /translation="MLNTLKPLINQLKKKHTYFILGIALLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIRYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(40934..42520)
                     /locus_tag="ONT85_000550"
     CDS             complement(40934..42520)
                     /locus_tag="ONT85_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HCR2377547.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVSQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQNEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHAALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQLLQETVEEIE"
     gene            42771..42995
                     /locus_tag="ONT85_000551"
     CDS             42771..42995
                     /locus_tag="ONT85_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HCR2377548.1"
                     /translation="MLSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            43137..44888
                     /locus_tag="ONT85_000552"
     CDS             43137..44888
                     /locus_tag="ONT85_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR2377549.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGVFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSIKIIFGWIFLLIIAAFLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     QMWEKQQLEAKIEGGEA"
     gene            44888..46672
                     /locus_tag="ONT85_000553"
     CDS             44888..46672
                     /locus_tag="ONT85_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR2377550.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGMYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYIRVKLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFLAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     VLTLFGIDALHSPVEVGLIYAFTTYVQGFFNPMSQMMDFLSIFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQMITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGI"
     gene            46998..47849
                     /locus_tag="ONT85_000554"
     CDS             46998..47849
                     /locus_tag="ONT85_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCR2377551.1"
                     /translation="MLIEKVLNNNVVITRDERNQEMIVMGKGLAFKKKVGDPIFDSCI
                     DKKFQLTNDGLSQQFQELAQAIPLEYLEISCEIIEYTKEALQVQLNDSVYIALTDHLY
                     TAIERHKMGIFVPNVLLYDIKQLFPKEYAIGKKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQDNTYLFTITIQDILNIVRYYYKITFDEDSVHFFRFVTHLRFFLTRVLD
                     GEKSEVGEVSEELFQVIQKKYKDTFGCVEKIADFLAETFNYQMSTDEKMYLTIHTARL
                     VHENRCK"
     gene            47903..49654
                     /locus_tag="ONT85_000555"
     CDS             47903..49654
                     /locus_tag="ONT85_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCR2377552.1"
                     /translation="MAKPSTNKLEGIEMGKYQELARKIVENVGGKENINGLTHCITRL
                     RFKLKNEEKANDEILKNMDGIVTVMRAGGQYQVVIGNHVPVVFEEVIKAGNLTFDEAV
                     STEKMRPFDMLIDGISGCFQPFLAILAAGGMIRGLTAFLVFLGAFDRGSGTFVMFDNI
                     GDSVFQFMPVIIGLTAARKFKVNEFVGMLLGAALMNPSLSLEALSGAAEAPLTTIFSG
                     TIFEAPIYQTVFGIPWIARNYASSVIPIIFIVLLASQIQKPIKKLVPEMIANFFVPFF
                     TVLITMPLGFLLVGPVFTFATDILMAGFETLLALSPVIYGAIVGFFWQILVMFGLHWA
                     IVPMGLMQFSVNGWQNIMTPVAVVSFGQTAALTALYFKLRNPKDKAIAIPAIVSGIVG
                     ITEPAIYGFTLPRKKIFIFTCVGGALGGAYSGLMNLTSWNQGGLGIFTIPNYIRPDGD
                     LTDVINVLIGIAIAMVVSFTLTFFFWKDEAGETDDIQKKSGKEIVKTPIQGQIAPLNA
                     AKDAAFAQGTLGRGILIYPEKGEVRAPFDGTVMTLFPTKQAIGMVSETGLELLIHVGL
                     DTVQLEGKYFESLVQQG"
     gene            49658..49828
                     /locus_tag="ONT85_000556"
     CDS             49658..49828
                     /locus_tag="ONT85_000556"
                     /inference="COORDINATES: protein motif:HMM:NF012578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCR2377553.1"
                     /translation="MKQGDLLLRFDKKKIEKEGYSLETPVIVTNYTDYLDILENQGET
                     VGPDDALITALT"
     gene            complement(49868..50770)
                     /locus_tag="ONT85_000557"
     CDS             complement(49868..50770)
                     /locus_tag="ONT85_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCR2377554.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQAIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIHDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSAMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVE"
     gene            51001..52404
                     /locus_tag="ONT85_000558"
     CDS             51001..52404
                     /locus_tag="ONT85_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HCR2377555.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPAMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEENGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKEEGIEVQ
                     DELARELSGNTHIMEEV"
     gene            52404..53837
                     /locus_tag="ONT85_000559"
     CDS             52404..53837
                     /locus_tag="ONT85_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HCR2377556.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGKDRFPV
                     ITGKKKMFEFDEDHFYPAKIAIDMYHRYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNEAGLKFYEDAFKECQKHGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEKGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIEMEPGDAALLKEHTVDFISFSYYASRVSTTDNELLEQTAGNIFASV
                     KNPYLQASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNQGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            53982..54908
                     /gene="trxB"
                     /locus_tag="ONT85_000560"
     CDS             53982..54908
                     /gene="trxB"
                     /locus_tag="ONT85_000560"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HCR2377557.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYESVSRFGTENAYGIVQDIKDHGSHKEVICEDKSYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNMPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            55122..56849
                     /locus_tag="ONT85_000561"
     CDS             55122..56849
                     /locus_tag="ONT85_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HCR2377558.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAKHSGLITIIGEEVDAAYLEEIKAV
                     TIDRELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPDGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYFAEKTISVTMSGQEGSAKISALMEKFRKEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGSSTEDAAEKVASYE
                     AIIKKMTEE"
     gene            56864..57214
                     /locus_tag="ONT85_000562"
     CDS             56864..57214
                     /locus_tag="ONT85_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HCR2377559.1"
                     /translation="MEKVVNFRSQSFFKLEVNGLPDTQKSKVEKVSQLFKMLSDPTRL
                     KILLYLKDGEQNVTAITQAVEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHV
                     LDILNQTLKHVEHQ"
     gene            complement(57306..58166)
                     /locus_tag="ONT85_000563"
     CDS             complement(57306..58166)
                     /locus_tag="ONT85_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HCR2377560.1"
                     /translation="MSSDSTEIVTFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLRQALQDALEEKALVLHTLINQELIEITRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEKSD"
     gene            58258..58686
                     /locus_tag="ONT85_000564"
     CDS             58258..58686
                     /locus_tag="ONT85_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HCR2377561.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            58848..59024
                     /locus_tag="ONT85_000565"
     CDS             58848..59024
                     /locus_tag="ONT85_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HCR2377562.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            59051..59497
                     /locus_tag="ONT85_000566"
     CDS             59051..59497
                     /locus_tag="ONT85_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HCR2377563.1"
                     /translation="MSLLSRLNDDMKAAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
     gene            complement(59651..61030)
                     /gene="brnQ"
                     /locus_tag="ONT85_000567"
     CDS             complement(59651..61030)
                     /gene="brnQ"
                     /locus_tag="ONT85_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HCR2377564.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGSNWI
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYDSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFQPMGNLNQSAADSYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMPLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKSPFVYQVTMFFSAVPAVLDM
                     LANSPALVSQQTVVASLLGFYHHHVPFAALGLGWMVPTLLGYAGSLLFYYAYRLSGYK
                     QEANELPEE"
     gene            61736..62707
                     /locus_tag="ONT85_000568"
     CDS             61736..62707
                     /locus_tag="ONT85_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HCR2377565.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            62731..64923
                     /locus_tag="ONT85_000569"
     CDS             62731..64923
                     /locus_tag="ONT85_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HCR2377566.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIICGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQKRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDAINKSYDEKIDKAKEDENIPKPTTEERIAALKKNFENVNAENVAFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEEQIQYLNVTPAQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSIV
                     LMKFFQLFQTEQATYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTIILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIVYQGMSFGD
                     ATTWLMLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICKQHHGTTLMRYFYIKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKTEVEKIKEEQEE"
     gene            64940..65416
                     /gene="ybeY"
                     /locus_tag="ONT85_000570"
     CDS             64940..65416
                     /gene="ybeY"
                     /locus_tag="ONT85_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HCR2377567.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLKLPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISIER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            65400..65795
                     /locus_tag="ONT85_000571"
     CDS             65400..65795
                     /locus_tag="ONT85_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HCR2377568.1"
                     /translation="MDSKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFGIL
                     ALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKDMA
                     AGAVLVTACFAVIIGLILFVPKIYHLLFN"
     gene            65810..66709
                     /gene="era"
                     /locus_tag="ONT85_000572"
     CDS             65810..66709
                     /gene="era"
                     /locus_tag="ONT85_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HCR2377569.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            66852..67664
                     /gene="recO"
                     /locus_tag="ONT85_000573"
     CDS             66852..67664
                     /gene="recO"
                     /locus_tag="ONT85_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HCR2377570.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRNIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKSGGVICEKHWPMDEHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRDLIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKTPEKNMPEKEIPEKEE"
     gene            68048..68965
                     /gene="glyQ"
                     /locus_tag="ONT85_000574"
     CDS             68048..68965
                     /gene="glyQ"
                     /locus_tag="ONT85_000574"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HCR2377571.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPENMKEAE"
     gene            68967..71042
                     /locus_tag="ONT85_000575"
     CDS             68967..71042
                     /locus_tag="ONT85_000575"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HCR2377572.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEVIPFEILDVKTGRASRGHRFLGEDVTFAHAKEYEEKLNEQFVIASPQKR
                     QEMIVTQANELAEKNHWVIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGELPQSTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISD
                     VISKDTDRFGIGLSEGQQQVIDFIKGRLRQLLTTKNIRHDVIEAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAELEKQSEVDPELFENEAEKELHKAVEA
                     MKKSFADQTINENYDQLVSLRPLIENYFDQTMVMAEEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
BASE COUNT        23266 a        12442 c        15206 g        20166 t
ORIGIN      
        1 tttgtctcag tccctttttt tgcatatttt tagcatttat ttcctaaaaa atgtaagggg
       61 attcctatat ttttaagtat acttgttttt ccttttatac attttatggt attatcattt
      121 tattagttat tataaaacaa tcgttagaaa ggacgtcatt atgcaagtat acggaattga
      181 tgcggtgctt cgaatcgtga gtcatttggc gtttatctac ttagccttct ggtctttacg
      241 ttcgttacgc attgaaactt ttttcagacc aatgcataat acgcaaatta gaatggcaat
      301 cgtacttttt tcaatttttt tagggtacac tgcaagtaat tttttcttag aagttatcgc
      361 tttatgccga aatcttttcg ttagcctgta aattttaaaa aaaggtacta ttttaaaagc
      421 aaggatgtta taatagaaaa gattttgagt aagtgtatgc tcattggaat tttttggagg
      481 gaacaacatg gaagagatca tcgtaagagg tggcaatcaa ttaaatggaa cagtaagaat
      541 tgaaggagct aaaaatgctg ttttgcctat tttagctgct agtttgttag cagaagaagg
      601 tattacgaca ttagacaacg ttccgattct ttctgatgtc ttcactatga atcaagtgat
      661 tcgtcactta aacgtagatg tagattttga tgaacaaaag aatcaagtaa caatcgatgc
      721 ttcaagacaa ttagaaatcg aagcgcctta tgagtatgtt agtcaaatgc gtgcttcaat
      781 cgttgtgatg ggtccgctac ttgccagaaa tggacatgca aaagtagcta tgcctggtgg
      841 atgtgcgatt ggtaaacgac caatcgattt gcatttaaaa ggatttcaag ctttaggagc
      901 aaaaattatt caaaaaaacg gctatatcga agcaattgcg gatgaattga tcggtaacac
      961 gatttattta gatttcccta gtgtaggtgc tactcaaaat atcatgatgg ctgccgtaaa
     1021 agcaaaagga acaaccatca ttgaaaacgt agctcgcgaa ccagaaatcg ttgatttagc
     1081 aaacattttg aataaaatgg gcgctcaagt ctatggagct ggtactgaaa caatgcgtat
     1141 tgaaggcgtg gatcatttac atgcagtcaa ccactcaatc gtacaagatc gtatcgaagc
     1201 aggtaccttt atggttgctg ctgcaatgac acaaggcaat gtgctgattg cagatgctat
     1261 ctctgaacat aaccgcccat tgatttctaa attgatcgaa atgggtgcag aaatcattga
     1321 agaagaaggc ggagtccgtg tcatcggacc aaaacatatc ttacctacag atgtaaaaac
     1381 gatgcctcat cctggtttcc ctacagatat gcaagctcaa atgacagcta tccaattagt
     1441 tgcagaaggt acaagtgtag tgactgaaac agtgtttgaa aatcgtttcc aacatttaga
     1501 agagatgcgc cgtatgaacg ctcatgtcaa aatcgacgga aatgtagcaa tcatggatgg
     1561 aaaccatgaa ttgcaaggtg cagaagtata tgcaacagac ttgcgggcag cagcagcatt
     1621 agttttagct ggcttgaaag caaatggaat cacacgtgtg cgtaacttga attatctaga
     1681 ccgtggatat tataatttcc atatcaaact acagcaatta ggtgcagacg ttgaacgtgt
     1741 agacatggat caaacttctg ctgaaaaaac agcacaaacg attgcttaag gaagtggctg
     1801 aatgagtaac caacgatata tcttattaac tttgataaag attttagtgg tgatcttatt
     1861 gcttatcctt ctttttgtgg ccggaacgat gatcggatat ggtgtcatcg gtggcggcaa
     1921 tccatttaaa gtattccagc caagtttgtg gattcatatt cgagactttt ttcattgata
     1981 aataagaatc acagtggttc tagtcgatat gaagtgagcc atgaagcaga acatgacaaa
     2041 agaaaacaga agggaacgtt gacgttagtc gcgatccctt tttttagtaa aagaaaagaa
     2101 ggaatctaat tgatgaagaa gatattgatt tatttagtta gaggatatca gcgctttatt
     2161 tcgcctgcat ttccacctag ctgccgttat tacccaacct gttccaatta tatgataaaa
     2221 gcaattcaaa cccatggtgc actaaaaggg acggtaatgg ggactgcacg gatcttacgc
     2281 tgtcatcctt ttgtcaaagg cggtatcgat tatgtaccat tgacattcca attaacgcga
     2341 aataagaaag ataaaaaaga accagattat aaaaaggttg tgaaaaatac gagtagcact
     2401 gagcgataaa gaaacaattt ggaaaatagc ttcttatatt ttgcaaaatt gtgagttatt
     2461 gccgaagtgt tggattttga acaccgtcaa aaaaaggttg tgaaaaatac gggtagcact
     2521 gagcgataaa gaaaaagagg aaaattattg tcaaagtaac aactccgcaa ctacttatat
     2581 gggggacaaa aaatgatcga agaactaaac aagaaagatt taccatggtc cttgttatta
     2641 gatgcagatc ctgataaaga aaaagttcaa gtatatgttt ccagagggag tggctggata
     2701 tggaaagaaa aagaaaaaac aataggtgta ctgatctatg tggcaaggga aaaagaatac
     2761 gaaatcgtca atgtagcagt agccccaacg tatcaaggaa aggggattgg cggaaaatta
     2821 cttgagacag cttttcagaa gctttcacaa ctcgcttcca gtcaaactca gatcatcata
     2881 cgaaccggca gtactagcag tgcggcgtta catctctatc aaaaaatggg ctatgtggag
     2941 attggtcgag tcaaagacta ttttatccat cattactctg agcccatttt tgaaaatgga
     3001 gatcaattaa gggatcaagt aattttagca attcacttac ctaaaaatag ctttaactaa
     3061 taaactttca attcgagaaa atgctttcaa atgctaaata gatacattac aatagcttta
     3121 gcaaacaaaa aggagaatat ttttatggga atattagttt ttgattttgg tggatcggct
     3181 gtcaaatatg gttgttggga tggcaaagag attaaaggta agggacaatt tgcaacgcct
     3241 gaatcttggg aagaaatgaa agcgcaatta tttcaagttt ataaaaaaat actcgtttct
     3301 tttgaagtcg aaggaattgg aatcagctct cctggagtag tgaataatga gaaacaggtg
     3361 attgagggaa tcagtgcgat tccttatatt catggattta atattttccg agatttggaa
     3421 actctgtttc aactcccagt aacgattgaa aatgacgcca actgtgccgg aatggctgaa
     3481 ttttatgaag gtgctgcccg tgattatcaa gatgttgctt ttgttgtggc aggaactggg
     3541 attggcgggg cgttgttcac gaaaggacaa atcaataaag gggcacatct ttatggtgga
     3601 gaatttgggt tgatgttctt aaatggcgat aagacattca gtaaacttgg tacagcggta
     3661 cagatggctt ggcgttattg tgaacgcaaa ggtctcgata aaaattctta taccggaaaa
     3721 gatgtctttg aattagcaga aactggagat gcaatagcaa aagaagaagt tgagagtttt
     3781 tatacgtatc ttacaaaggg attattcagt attcaatttt cgtttgatcc agaagtgatc
     3841 gtattaggcg gcggtgtttc tgcaaaagaa gggctcattg atgagatcaa aagccgaatg
     3901 aagaagctga cagaacaatt cgatctgcat gatttcgaac cgcagatttt attatgtgaa
     3961 tacagaaacg atgctaatct tgtaggtgca gcagcaaact atctcgctgt acgtcaaggg
     4021 gagaattaag agctttagag aaggtgcagc ttgcataaaa gaagtgaatg aaatgaatag
     4081 gagattttta cctaaggaat gttttatttg ctgcagtatg ataaaatatc tgagaggtga
     4141 aaatgatgtc aaaaaaaaat aaagatgttg aagtgcgtat agaagaagtg aaaaaagata
     4201 tcaaaggtaa gaattatgaa gtcactcagt tatttatagg gaaaaaaatg attggtgaga
     4261 tcttagccta tgggccaaaa gaatatgaaa ttttctttgg tgaagaagat tttggaaaag
     4321 aaaaaagtct tgaaaatgcg atagaaacag tgattcgaca ctggaatctg cacgaataaa
     4381 aaaatatcaa aaaaactttc aataaagggt tgcaaaaatc tggaaaatta ggtattataa
     4441 ctaagtcagg ttgatacaaa cctttcggag gagtagcgaa gtggctaaac gcgacggact
     4501 gtaaatccgt tccttagggt tcagtggttc gaatccactc tcctccattt cattggggta
     4561 tagccaagcg gtaaggcaag ggactttgac tccctcatgc gttggttcga atccagctac
     4621 cccagtatac cacaatcgta agctctcgtt agttcgagag ctcttttttt tgctttgcta
     4681 ccttgttcct tgaagattct gctgatttaa gatagagtta agttaactac aagaattgga
     4741 gggatccaca tgaaatggaa agaaaaagta gacgaagcag ctgaaaagtt atatgatttg
     4801 gtcaaatcag aaaaatacaa tattgaagtg aatattccga aaaaaggcgg aaaagcggtt
     4861 caggtaaaat caaaacgccc aacgaatcat acaaaaaaat ggatggctaa aaaccgctaa
     4921 taataacaaa agatccataa gtcggcattt tcatcgattt tagggtcttt tgttattttc
     4981 taggttgaac atatctaaaa aattgtttct gacaatagga gtaggaagaa cgatgtgtgt
     5041 ctaaatcctc tgttgatgga tagaaacctt tatctttttc agttgtgaat caggtatata
     5101 ccagttataa tagaagaaac gaaaggacgg gtgtacgtta tgtcgaatca attacctgta
     5161 tatatacaga ttcatgacca attaaaaaat gaaatcgaac aaggtatatg gaaagtcgga
     5221 gaccgtcttc cttcagaaag agaattagca gtgaaattca atgtcagccg tatgacgttg
     5281 agacaagcaa ttcaaaactt ggcagatgaa ggaatcttag aaagaaagat cggttcgggt
     5341 acatatgttg cgcgagaaaa agtacaagaa aaaatgtctg gtgcaacaag ttttacagaa
     5401 attatggaat cgcaaaatcg agtaccgtct agtcggacaa tctcttattt cttgacttcg
     5461 ccaagctcaa gtgaaatgga aaaactaaac ttgagtaaag atgacacgat cttgcgaatg
     5521 gaacgtattc gtttcgcaga tgatgtccct atttgttttg aagttgccag tgtgccacaa
     5581 aagctgatcg atggctacag caaagcagag attactgaat cgttgtatcg cacgctagaa
     5641 gaaaaaggcg ggaaaaagat cggtccagcg aatcaaacga ttactgctgt gttggcttct
     5701 gagaaaattg ctgactattt agaggtaaaa cgaggagatg cagttcttcg tttgcgccag
     5761 atttcttatt ttgaagacgg tacgccgttt gagtatgtgc gtacgcaata cgtaggaaat
     5821 cgttttgagt tttatttgga aaaataaaaa acaggtgatc cctgattcct tacttagaaa
     5881 taaaagagaa tcagaatcac ctgtttttat atatgatcag ttaccagttg ctcgtgataa
     5941 gaaacaggac cctcttcaaa ttggacttgt ccgttcagca gctcaatgat ggattcttta
     6001 aagtggtcag cacttttttc atctacagca cagatgacgc taacttgatc agtatattgg
     6061 atttctttta taaaaaaatg ttggagttca agataattct gtaattttcc tagttgcgga
     6121 tacgtgatca tcacagagat ctcctgttgc aaggtacctt ctacgattcc aatgtcagac
     6181 aatgcttgag agacagcatg agtgtatgct cgtattaatc cgcctgtgcc gagtttcgtt
     6241 cctccaaaat atcgagtgac tactgcagct acattgatca gttgattttt tttgaggact
     6301 tccaacatcg gtacgccagc cgttccactt ggttctccat catcgctgct tcgctggatt
     6361 tcattttttt ctccaatgat gaaggcgctg cagttatggt tcgctttcca atgttctttt
     6421 tttatttctt gaatgaaact ttttgcttgg tcctctgatt ctattcgtct aagagaacag
     6481 ataaagcgtg attttttgat ctcaatctca ctttgtccat tttcttttat tgtacgatag
     6541 cgctcgatca tcttgtcacc tctttttttc attttcttta ttcagtataa ataaagttct
     6601 aggttgtttc aacactgtaa cggttgcgca atcgctgtag tatgcgataa taaagacaaa
     6661 ggtaggtgga tctggggatg caggaacgaa tgaaaaagta cgatgcaatt acgcattatt
     6721 tgaagaacaa tggcggctct caagtaacgt tgacctttac ccaatttgac gaactgctgt
     6781 ttcccagtaa cggacttccc aaaacagcaa gggaaagtac agattggtgg gcaaatgatt
     6841 ataaacaccc tgaaaaaggt gcctatggct ggataaatgc aggctatgaa gtagttgtca
     6901 tcaatttgga caaagaatat gtcgtgttca ataaattagt gaaatctagt tggctatttg
     6961 attaaataaa ccgcgtttag aaaacgttga caaatttctt tctttttata taatagagta
     7021 acagacgcga agaaaacgtt tgcgtagttt gttttaaaaa ggagagaaaa gtatggctaa
     7081 aaaaacaatc atgcttgtat gttcggctgg tatgagcaca agtttactag taacaaaaat
     7141 gcaaaaagct gctgaagcaa aaggaatcga ctcggatatt ttcgctgtgt ccgcttctga
     7201 cgcagataac aatcttgcaa ataaagatgt agatgtactg ttattaggac ctcaagttcg
     7261 ctttatgaaa gctgattttg aaaaacgtct ggaaccaaaa ggcattcctt tagacgtcat
     7321 caatatggct gattatggta tgatgaatgg cgaaaaagtg ttagatcaag caatcacgct
     7381 cataggtgaa taaatagaac agtataacta tcgaacaagc gatccaaaaa atagatcgct
     7441 tgttttttcg tatatttttt tagggcgaat aatatgaatc atttttgggg tagaaggctt
     7501 ctgacgagag atattaaagc aatagactgt gaggaggtaa atttaccaaa ttatgagaag
     7561 atcgcagcag tggaacgttt gggcaactgg attctttgtc atcgctgtgg aatgaggacg
     7621 tccgtcattg atggacagct tgcagattac ggttattttt gtacacattg tctatattta
     7681 gggcgatgcg acactcaaca agatttatat ttgtttgatc agcctgaagc aaaacctcga
     7741 gaagtcgtct ttttttggac aggacagctt actgaaaaac aaacggaaat cgcaaagaga
     7801 atactttgcc attcagaaaa aagacatcat cttatttggg ctgtaacagg tgcagggaaa
     7861 acagagatgc tttatcctat tttagtggaa actttaaaag ctggaggaag agtagcgatt
     7921 tgtacaccgc gagtcgatgt ttgtaatgaa ttgtttttac gctatcgtta tgtttttcca
     7981 gaagaaaaaa tcacgttgct ccatgggaat acagcaacgg cttatacctt tacttctttt
     8041 gttatctgta cgattcatca gctataccgt ttttatcggt cctttgattt aatagtgatc
     8101 gatgagatcg atgcgtttcc ttataacggg gatgcaggat tagctcatgc tgtgcaaaca
     8161 gcgattaaac cagacggacg attcatctac ttaagtgcca cacctgataa aaagttacta
     8221 aaggaaatca agcagacgtt tgacatccac aaacttgctt tgcgctttca tcggcgttta
     8281 cttcctgaac ccgtcttggt tttcatgaat aattggcgac aaaaatgcaa gaacaagaag
     8341 aagataaaat ctttggtgcg gatcattcat caattaatta aagaaaacca tatcctttta
     8401 ttttgtccaa gtatcacgat gatgcatcgt ttgaaagcca cacttcaaga ggctcttccg
     8461 agatatgaga tcacagatgt ttcttcaaga gacaatcagc gggcggaaaa agtccagaaa
     8521 atgcgagaag caaaatatga catactgtta accacgatga tccttgaacg tggagtcaca
     8581 tttgaaagaa tatccgttat cgttttagga gcggatcatc cggtttttac taaatcttct
     8641 ttggtacaaa tcgcaggaag agccgatcgt aaagggcctt atacgaacag tcaagtatac
     8701 tttttctatg aagaaaaaac tgcagctatt cgaaaagctt gtcaggaaat caaagaaatg
     8761 aatgaagaag ggaagaagat gctgtgaggt gcagttgctg cagtaaagat atcgtccaaa
     8821 atttaacatt cagcgagatt attggtgttt cgaaatacag attacctcag acttgttcag
     8881 tttgtcgcgc acaatttcat ttattggaaa caagaggctg ttctggctgt caaaaaccaa
     8941 tccatttcaa tctagagagt acgaaaaaga aagtaaagtc tattcccgtt tactgcagtg
     9001 actgtcaaga atggaagaag aaatatccga cctatgattt tcagcaccgt gccttttttg
     9061 tgtacgatga aaatatgaag gcctggctca atcaatacaa gtttatggga gatatccgac
     9121 tagcaggcac ctttgcatat aaatggacgc agataaaaaa agaatacccg acatacatct
     9181 attgtccaat acctttatct gaaaaaaggc gaacagaaag aggattcaat caagtaagcg
     9241 agatgttgaa agcagcaaga attccttttc agatgctact caaaagagag aagcatctgc
     9301 tggctcaagc aaaaaacacg cgaaaggaaa gattggagat gcctcaacct tttgaacttg
     9361 cagtcacctt ttctaagata caaaataaaa aaatattgat tattgatgat gtctatacaa
     9421 caggacagac tcttttccac gcagcagatt gtttgcaggt cgcacagccg aaaatcatcc
     9481 gaaccatttc tttagcaaga tgaattagat gtggaaattt gatagcgatt taatttgcaa
     9541 ataacctaga gttcgatata atagaattaa gaagagggaa agagtggtcc ttctccttct
     9601 tgattgtggt agacgaaagg ggtaattttt atgtttagat ataatgtacg cggcgaaaat
     9661 atcgaagtaa ctgaagcgat tcgcgactac gttgagaaaa aagtaggcaa actagaacgc
     9721 tatttcagtg acgcacccga tgcaacggcg catgtgaact taaaagttta tacagaaaaa
     9781 acagcaaaag tggaagtaac gatcccatta ccttatcttg tattacgtgc cgaagaaact
     9841 tctccagatt tatacgcaag tatcgatttg gttgttgaca aattggaacg ccaaatccgc
     9901 aaattcaaaa caaaaattaa tcgtaaatcc cgtgaaacgg gcatgaatac tgcagatgct
     9961 actgctttat tcaatgaaga ggacgaaaac gaaaatgatt ctgaattaga tatcgtacgt
    10021 acgaaacgcc tttcattgaa accaatggat agtgaagaag cagtcttaca aatgaacatg
    10081 ctgggacata acttctttat ttttgaagat gcagaaacaa atggtacaag tatcgtttac
    10141 cgccgtaaag atggaaaata cggactgatt gaaacagact gatcagcctt acttaaataa
    10201 caacgaaaac ttgcagcagc tactgacagc tgccgcaagt ttttttgagt ttcctcctct
    10261 tttagatgaa atatgaataa attatgaagt tcttacttta atacgcttaa gatatttcaa
    10321 ttatctataa ctaatgataa aatgaaaagt aaagtctaca tagtctggat aatacacaga
    10381 aggaaaagga gattacacaa cgcatggcca atttgattcg ttcacttatc gaaaacgata
    10441 agaaagaact aaaacgttta gatagcattg caaaaaaagt tgaagcatac gctgaccaaa
    10501 tggcagcatt aactgatgaa caattacagg caaaaactgc tgaattcaag ggcagatacc
    10561 aaaaaggaga aacattagat caattacttc cggaagcttt tgcagtcgtc cgagaagcag
    10621 caaaacgtgt attggggtta tatccacacc acgtacaatt gatgggtgga atcgtgcttc
    10681 acgatggaaa tatccctgaa atgcgtactg gtgaaggtaa aaccttgacc gcaacgatgc
    10741 cagtatatct aaatgctctg actggcgaag gggtacacgt agttaccgtc aatgaatatt
    10801 tagcaacccg tgactcgacg gaaatgggcg aactttataa tttcttagga ttaacagtgg
    10861 ggttgaatat caactcaaaa tcttcggatg aaaaacgcga ggcttataat tgtgatatca
    10921 cttatagtac gaataatgaa ttaggatttg actacttgag agacaatatg gtcgtttacc
    10981 gccatcaaat ggtacaacgt ccgctgaatt atgccattgt cgatgaagtt gactcaatct
    11041 tgattgatga agcgagaacg ccgctgatca tttcagggca agcagaaaaa tccacagctt
    11101 tatatacaag agcagacaac ttcgtgaaac gtttgaaaga agaagaagat tataaaatcg
    11161 atgtacaatc aaaaacaatc agtttaaccg aagcagggat cgaaaaagca gaagaaacat
    11221 tcggtttgga taacttatac gacattgaaa atactgcatt gacccatcac ttggatcaag
    11281 cattgagagc caactatatc atgcttttag atatcgatta tgttgtccaa gacggaaaag
    11341 tactgattgt tgaccagttc actggtcgga tcatggatgg acgccgttat tcagatggac
    11401 ttcaccaagc aatcgaagct aaagaaggcg tagagatcga agacgaaacg aaaaccatgg
    11461 cgacgatcac tttccagaac tatttccgta tgtacaagaa acttgcggga atgactggta
    11521 cagctaaaac agaagaagaa gaattcagag aaatttataa catccaagta taccagattc
    11581 cgacaaatcg tccggttatc cgtgatgatc gtccagattt gctttaccca acattacaaa
    11641 gcaagttcca cgctgtcgtg caagatatca aagatcgcta ccgcaaaggt caacctgtgt
    11701 tagtcggaac tgttgcggtt gaaacatctg agttattatc agatatgctg aatcaagaac
    11761 gtattcctca tgaagtgctg aatgcgaaaa atcactttaa agaagcagag atcatcatga
    11821 atgctggtca aaaaggtgct gtgacgattg ctaccaacat ggctggtcgg ggaacagata
    11881 tcaagttagg acttggggta agagaacttg gtggtctagc agttatcggg acagaacgac
    11941 atgaatctcg tcgtatcgat aaccagcttc gaggacgtgc aggacgtcaa ggagaccctg
    12001 gtatgtcaca gttctacttg tcattggaag atgatttgat gaaacgattc ggttcagaac
    12061 gaatcaaatc tttcttagat cggatgaaga tcggtgagga agatgctgtt atccaaagca
    12121 agatgctgac gaaacaagta gaatctgcac aaaaacgtgt agaaggaaat aactatgaca
    12181 cacgtaaaaa cgtcttacaa tacgatgatg tgatgcgtga acaacgtgaa gtaatctata
    12241 aacagcgaca agaagtaatc atggaagaaa aaagcttatc caagccacta atgaacatgg
    12301 tcaaacggac aatcagtcgt gtagtagatg cgcatacaca attagagaaa gaaaattgga
    12361 atttggaaag tatcatcgat tttgccggca gtactcttgt ccatgaagat agtattactc
    12421 ttgcagatat cgaaggaaaa acagctcagg agatcaaaga gtatctaaac gaacgagcac
    12481 aagaagtatt taataccaaa gcggctcaac taaatggtcc agagcaactg ttggaatttg
    12541 aaaaagtcgt gatcttgcgt gttgtggact ctaaatggac agaccatatt gatgcaatgg
    12601 atcaattacg tcagtcaatc ggcttacgtg cttatggaca gaacaatcca ttagtcgaat
    12661 atcaaacaga aggctataag atgtttgaag atatggtagg cgccatcgag tatgaagtca
    12721 ctcgaatctt tatgaaagct gagatccgtc aaaacgtaca gcgtgaacag gtttctcaag
    12781 gaacagcttc tcactcagaa gacggagatg ccaaagaaaa tagtaatact agcgcgaaaa
    12841 aacagccaat ccatgtgaac aaggtcggaa gaaatgatcc ttgtccttgt ggaagcggga
    12901 aaaaatataa gaactgtcat ggaaaaaatg caaactaatt tctgacagca ggattggtga
    12961 gacaaattct ttttttaaga attcgtctca ccttctttct gttatttgct gattctgtta
    13021 gactagatat aatgaaaaaa gtatagtgaa agaaggaaaa aacatggaaa tatccgaaat
    13081 tcgaaatctc ctgacagaaa tgcaggaaaa ggtcacaagc ttcaggaggt ctctttgact
    13141 tagaccagct ggaagaagac attgcggaag cagaagcaaa aatggctgaa cctggttttt
    13201 gggatgatag cgaagctgct caaaaagtga tcagtgaaaa caatgcaaat aaagaaactt
    13261 atgatcaatt ccattctctt gctgaagaac tggaagaaat cgaattgatg gtggagatgt
    13321 tgcaggaaga acctgatgca gccatgcaag aagaagcaga aacacgtatc aatgcgctag
    13381 atgaaaaaat gaagacatat gaattatcta tgttgttaga tggtccttat gatcgaaaca
    13441 acgccatcgt tgaattgcat ccaggggctg gcggaacgga gtcccaagat tggggcagta
    13501 tgcttttacg tatgtataca cgctgggcag agcagcatgg gtatcaagta gagacactag
    13561 attatcaagc aggtgatgaa gcaggaatca agagtgtgac cctactgatc aaaggacaca
    13621 acgcatttgg ttatctgaaa tcagaaaaag gtgttcatcg ccttgtacgt atctcgcctt
    13681 ttgactcagc gaaacgacgt catacttctt tttgttcggt agatgtgatg ccagagcttg
    13741 atgaaacgat cgatatagaa atcaatccag atgatttgaa aatcgatacg taccgagcaa
    13801 gtggtgcagg tggtcagcac atcaataaaa cagaatctgc tgtacggatc actcacttgc
    13861 ctacaggaac agtagtagct agtcaggcac aacgttcaca attgaaaaac cgtgagcaag
    13921 cgatggggat gttaaaagct aagctttatc aattagaagt ggaaaagaaa gaacaagaag
    13981 cagctgcact tcgaggagaa caaaaagaaa tcggctgggg ttcacagatc cgttcttatg
    14041 ttttccatcc atattctatg gtaaaagacc atcggacaaa cgtagagaca ggaaatgtcc
    14101 aagcagttat ggacggtgac cttgatttat ttattgacgg ctatttaaaa atgcatctac
    14161 aataagaacc cattgttaaa ttgaaataaa attgtaagat tttgcaagca tgttgaaaca
    14221 ttgccatgct ataatagcta aagactgaaa aagatatgga gagaacgcca tgattgaaat
    14281 gaaggatgta atgaagaagt actcaaatgg tacgaccgct atccgcaatc tctcaattgt
    14341 catcgaacaa ggagagtttg tctatgtggt tggtccgtct ggagccggta aatctacatt
    14401 catcaaattg atgtatcgag aagaaaaagc ttcaaaagga actttgaacg tcgcaggtca
    14461 tgatctaata gcaatcaaaa accgcgaagt tccttatcta cgcagagaaa tcggcgtggt
    14521 gttccaagac tataaattat taccaagaaa aacagtatat gaaaatgttg cgtacgctat
    14581 gcaagttatc ggcagaaaac ctcgggatat caaaaaacga gtaatggaag tgctggacct
    14641 tgttggtttg aaacacaaag ttcgagtctt tcctagcgaa ttatccggtg gggaacagca
    14701 acgtgtctct attgctcggg caatagtgaa tacgcctaaa gtacttatcg cggatgaacc
    14761 gacaggaaat ctggatccag aaaactcatg ggaaatcatg aaattattag atcggatcaa
    14821 tgcccaagga actactgtcg tgatggctac acataacagt acgatcgtaa atacgattcg
    14881 gcaccgagta attgctatcg aaaacggccg cattattcga gatcaagcgg aaggggaata
    14941 tggatacgat gattagaacc tttttccgcc atttattaga aagtatcaag agtttgaaac
    15001 gtaacggctg gatgacagtt gcttcagtca gtgcggtaac gatcacattg actttagtcg
    15061 gtgtattcat ggcagttatc atgaacgcaa ctaaattagc tcaagatata gaaggaaacg
    15121 ttgatgtctc tgtattcgta gatatcggta caaaagacga agagatgaaa aagctagaaa
    15181 aagaattgaa tgacttagat catgttaaaa aagttgaatt ttcaagcaaa gaacaggaat
    15241 tgaaaaaaat ccaaaacgaa atgggtgatt cctggaaatt gtttgaaggt gacaataacc
    15301 cgttatatga tgtgtatgta gtaagtgcta ctgatccttc ttatacaaaa caagtagcag
    15361 aagaagcagc agatttgaag aatgtttctc gttctgatta cggtggagct tcctcagatc
    15421 ggattttcca aatcgctggt gcagttcgga catggggatt agttgcagcg gcattgctgc
    15481 tgttcgtagc gatgtttctc atttcgaaca cgattcggat cacgattttg tctagacagc
    15541 gtgaaattca aatcatgcgt ttagtcggtg caaaaaatgg atatattcgc tggccgtttt
    15601 tccttgaagg tggctggatc ggtctgatcg gtgcgattct tccgattttg atcatcacat
    15661 ttggttatgc atggtttttc caattgatca atccatcatt attacgttcc cattattcat
    15721 tgattcatcc acaaaatatc gtttggaaaa tcaatctttt aatggtcggc attggtgtga
    15781 tcattggttc acttggctcg atcatttcta tgagacgttt cttgaaattc taataccaca
    15841 atataaataa aatgagaaag ttggacagtt tcgttccagc tttctttttt tattggctag
    15901 gagaatagaa ggaagcggct tttttccctt tactttaaaa aacaacttgg gtattcttaa
    15961 gttggtagaa gaagaggtga agccgtgaaa attatcgttg acaatttttt tacgatcctt
    16021 ttgattatga atattttgct ttcctttatc atcgtttttc gagaaagaaa ggaaactgct
    16081 caaacatggg catgggtgct cgttttgatg ttcattccag ttgtaggatt tattttatat
    16141 atttttttag gaagagggat ttccaaagag aaaatctttg atttgaagat ccaagggaaa
    16201 atcggtaaga acctggaaat tgaagaagac aaacaggcat tgatgcgagg gctttatcct
    16261 catccgccta ctggacaagt agatgtgaaa caattgatct atatgctcac ggtatttgaa
    16321 agtacgctct atacgactaa aaatgaaatc actttattta cagatggtcg tgaaaaattc
    16381 gatgcgctga ttgaagatat caagcaggca gaagaccata tccacttcca gtattatatt
    16441 tatcgcagtg atgcattagg tgaagaagtt cgagatgcat tgacagaggc ggcaagacga
    16501 ggggtaaaag tcagagtcct tcttgatgca tggggttcca cacaagtttc ttccagcttt
    16561 tttcagaatt tgaaaaaagc aggcggagaa gtcgcatttt tctttcctct atttgttccg
    16621 tatattaatc ctcggatcaa ttatcgcaat catcgtaaaa tcgtagttat tgatgggaaa
    16681 atcggatata caggtgggtt caatgttgga aatgaatatc taggattggt aaagaaattt
    16741 ggttattggc gagacaatca tttgcgtatt tacggagaag cggtgtatac cttgcaaaac
    16801 cgttttttga tggactggaa ttctcagcat acaaaagaag taggctattc tcccaaattt
    16861 tttccgtcca ttcactctac tggtgatata gctgttcaaa tcgtgacaag cggaccagat
    16921 accgaacatg aacaaatcaa aatgacttat ttgaaaatga tttcgttagc caaacgggag
    16981 atattgatcc aaacgcctta ttacataccg gatggatcga ttcatgaagc actgaagttg
    17041 gctcttcttt caggcgtaaa agttcatatc caaataccta acaaaccaga tcatcttctc
    17101 gtttactggg caacgtattc tttcgctgca gagctaatcg agtatggagc aaggatcgaa
    17161 acatatgaga atggatttat tcatgcaaag acgatgatta tcgatggtgg cattgtttca
    17221 gttggctcag caaatatcga tgttcgttca tttcgcttgg actttgaggt caatacactg
    17281 gtttatgatg aacgaatggc ttcacgtgta agaaaagctt tttttgaaga ctcgaaaatt
    17341 tctacgcatc tcacaaaaga aatgtatgaa aaccgaggaa taatcatcaa gatgaaagaa
    17401 ggattagcaa gattgatttc gccgctcctt taaaacataa aaaggacatg agaaccatca
    17461 acagatggag atctcgtgtc cttttctaac ggggccattt ctgagagtac gtcagaaatg
    17521 gagaaagcgt ctcgcatgag cgaaattaac tttggggtat ctcttgtttt ttggggaaag
    17581 caagaaataa gctaatatat gctattaaaa gagacacatt tcaaagccag tatagcatag
    17641 aaacttttaa tgagatagtt ttttttacaa ttatgagaat ttattttgtt atttaatgat
    17701 tttgattcgt tttcatcaag taatgaaaat gataaaataa acatacttta ggattaaagg
    17761 agatttctaa tgaaaaaact taaattattc ttaccaataa taggattagc tattctttta
    17821 tcaggctgtt caaagtggat tgatcgagga gaatccatta cggctgttgg ctcttctgct
    17881 ttacagcctt tagtagaaac agttgctgaa acttaccaga cagaaaatcc tggaaaattc
    17941 atcaatgtac aagggggcgg aagcggaact ggtttgtctc aagtacagtc aggagcagtc
    18001 gacatcggaa actctgattt gtttgcggaa gaaaaatcgg ggatcgatgc ctcagcatta
    18061 gtcgatcatc gagtagcagt tgttgggatc actcctattg tgaacaagga tattggcgta
    18121 aaagacatca gtatggaaaa cctgaaaaaa ctatttacag gcgagatcaa aaactggagt
    18181 gaagtcggtg gaaaagatca gccagtagtt atattgaacc gcgcttcagg aagcggaacc
    18241 cgtagcacgt ttgaaaaatg ggtattggac ggagaaacag ctgttcaggc acaagaacaa
    18301 gattcaagcg gaatggtgcg ccagatcgtt gccgacacac ccggtgcaat cagctatgtt
    18361 gctttttctt atgtttccga tgatgtagat acgttgaatg ttaacggtgt agaaccgaca
    18421 gatgaaaatg tgacaacgaa caaatggacg atttgggcat atgagcatat gtacacaaga
    18481 gatcatccaa aaaaactgac aaaagaattt ttagactata tcttatctga tgaagtacaa
    18541 gataacatcg ttggagaact aggttatatc cctgtttctc atatgaaagt cgaacgtgat
    18601 tggcaaggaa atatcgttaa agagtaatct ttacacaaaa tttacaaact tagctaaact
    18661 tttacatgtt attaaaacca aattgatgtt caatttgtac actgagtgag tatgttaaac
    18721 ctgattaagg gggagtcatc tgtggaagat gtgaaaaaag tattattaac aaaatcaaaa
    18781 cgttcaaaaa tggaacagcg tggaaaattc atcagttttt tatgtatcgc gctgatcgtg
    18841 ttggttgtat tatctatttt ttattttgta gctagtaaag gattggccac cttttttact
    18901 gataagatca atgtatttga ttttctgttt ggtacagatt ggaatccaag ccaaatcgga
    18961 gcaaatggga aaccaatggt aggggcattg cctatgatca caggttcgtt tattgtgacc
    19021 tttttatctg ctatcgttgc cacgccattt gccattggtg cagcagtatt catgacggag
    19081 atctctccta aaaacggaac gaagatctta cagccggtca tcgagctgct tgtaggtatt
    19141 ccttctgtcg tttatggttt tatcggttta tcagttatcg tgccttttgt cagaagtatt
    19201 tttggcggga ctgggtttgg tatcttagct ggtacatttg tgttgttcgt tatgatcttg
    19261 ccgactgtca caacgatgac tgttgatgca ttaaaggcag tgccacggca ctatcgggaa
    19321 gcttcattag cgcttggtgc cacaagatgg caaacaatct acaaagtcgt gctacgagca
    19381 gccgtgcccg ggattttaac agcagtcgtt tttggtatgg ctcgtgcgtt tggtgaagcc
    19441 ttagctatcc aaatggtcat cggtaatgca gcattgatgc caacaggtct tactactcct
    19501 gcttctacat tgacgtccat cttaacaatg ggaatcggaa atacaatcat gggaacagtt
    19561 gaaaataatg tgttatggtc attagcgctg atcttgctat tgatgtcatt attctttaat
    19621 atcgtgatcc gtatgattgg gaagaaagga gagctgaaat aatgaacgca aaacgctcag
    19681 ataaaattgc tacaggtgtt ttatatatag tttcggggat catcgtattg attttagcag
    19741 ctttactact ttatatctta gttcggggaa taccgcacat ttcatggtcc ttcttgaccg
    19801 aaccttccaa agcctaccaa gaaggcggtg gaatcgggat ccagctattt aactcgattt
    19861 atttgctttt gatcacgatg atcatcagtt ttcctatttc tttaggtgcg ggtatctatc
    19921 tttcagaata tgctggaaaa aactggctaa cagatgtgat tcgcacgtcg attgaaatct
    19981 tgagctcgtt gccttctgtc gttgtcggtt tattcggttt cttggttttc gttattcaat
    20041 tccagtatgg tttctcgatt atttctggtg cattggcttt gactgtattc aacttgcctc
    20101 ttttgactag aacagttgag gagtctttac aagcggtcca ttacacacaa cgagaagcag
    20161 gattggcctt aggtttgtca cgctgggaaa cagtgataaa agtggtggtt ccagaagcga
    20221 caccagggat tttgactggt gtgatcctaa gttctggaag aatttttggt gaagcagctg
    20281 cattgatcta tacggctgga caaagtgcac ctgcgttaga ttttacgaac tggaatccat
    20341 taagtgtttc aagcccgatc agcattttcc gtcaagctga aacattagct gtccatattt
    20401 ggaagatcaa tacagaaggc acgatgcctg atggtgtttc tgtttcggct ggcgcttctg
    20461 ccgtgttgat tattgctgtt ttactgttca acttcggcgc tcgtgcgatt ggcaaacgat
    20521 tataccgaaa aatgacttca gcctaaggag aagaagcaaa atgaaaaaat ataactggaa
    20581 tgaaacaaat gtcatcacca tgccaaaaga gaaagaagta gcactatata cggatgatct
    20641 acatgtctgg tatggcgaca atgaagcaat caaaggagtg gatctggaat ttgaaaaaaa
    20701 taagatcact gcactgattg gtccttccgg ctgcggaaaa tctacgtatc ttcgttcgtt
    20761 gaatcgaatg aatgatggga tcgctaatac gaaaatcact gggaaaatca tgtatcaagg
    20821 cgtagatatt aatgcgcctc aagtggatgt ttatgagatg cgcaaacgga ttggcatggt
    20881 ttttcaacgt cctaatccgt tcagcaagtc gatttatgac aatattacat ttgctttgaa
    20941 acagcacggc gaaaaaaaca aaaagaaatt agatgaaatc gttgaaacaa gcttgaaaca
    21001 agctgccttg tgggaccaag taaaagacag cttggacaaa agtgccttgg ctttatctgg
    21061 cgggcaacag caacgtttat gtattgctcg tgcgatcgcg atgaaacctg atatcttgct
    21121 gctagacgag ccggcaagtg ctttggaccc tatttcgaca ggaacggttg aagaaacact
    21181 tgtaaacttg aaagaagact atacgatcat catcgtgacg cataacatgc aacaagcagc
    21241 tcgtatcagc gactatactg cctttttcta tttaggaaaa gtaatggaat atgacaaaac
    21301 taacaagata tttaccagac caaaaattca ggcaaccgaa gattatgtgt ccggtcattt
    21361 tggttaagac ggaggcctca acatgacaaa agagattatt tcatctaaag atcttcatct
    21421 gtattatgga aagaaagaag cactaaaagg gatcgatcta agcttcaatc aaggggaaat
    21481 cactgcgatg atcggtcctt ctggttgcgg aaaatccact tatctgcgtt ctttgaaccg
    21541 gatgaatgac ctgattccga atgtaacgat caccggaagt gttttatata aagacaaaga
    21601 catctatggt ccaaaaacag atgttgttga actaagaaaa gaaatcggca tggtattcca
    21661 gcagccaaac ccatttcctt tttctatcta tgaaaatgtc atctatggat tgaaattaaa
    21721 aggggaaaaa gataaaaaag tccttgatca agtagttgaa gaaagcctga aggcagcgtc
    21781 tgtttgggat gatgtgaaag acaaattgca taaaagtgct ttatctttat ccggtgggca
    21841 gcagcagcgt gtatgtatcg cacgtgtttt ggcagttaat ccggaaatca tcttattgga
    21901 tgaaccaaca agtgcgcttg atcctgtttc tactgggaaa atcgaaagta tgctgttaga
    21961 attaagagag cggtatacga tgatcatcgt gacccataat atgtctcaag cttcacgtat
    22021 ttcagataag acggcattct ttttagatgg tcacttgatc gaatacaatg atacgaagaa
    22081 gatattcatg aatccagaaa aacaagagac agaagattat atttcaggtc gatttggata
    22141 aggaggaaac ggaatgctac gtacacaatt tgaagaagaa ttgctgaatc tccataatca
    22201 gttctacgaa atggggatga tggtgagcag cgccgtgcac aagtcagttc gtgcatacat
    22261 caaacacgat aaaatcttag cccaagaagt tatcgaaaac gatataaata tcaataacat
    22321 ggaaacacgc ttggagaaga aaagttttga aatgatcgca ttgcaacagc cagttacaac
    22381 tgatttgcgt atgatcatta cggtcatgaa agctagctcg gatttagaac gtatggccga
    22441 tcatgcggtg tctgttgcta aatccacgat tcgcgtaaaa gggcagacga gaataccgga
    22501 aatcgaaaaa gagatctccg atatgtccga ttatgtgaaa aaaatggtgg acaacgtcct
    22561 agttgcttat gtcaaaacag acgaaaaaga cgcacgtacg atcgccaata tggatcaaag
    22621 agtcaatgac tatttcaatc gaatctataa tgcaacaatc aaaaatatgc aggaaaatcc
    22681 tgagacagtc atcagtggaa cagactactt gaatgttgcc agttatctag aacggatcgg
    22741 agactatgtg acaaacatct gtgaatggat cgtctacttg gctacaggga aaatcactga
    22801 gttaaatacg aaccacaatg agattttcta aactgaagct gtgattatgc cgtttgttta
    22861 ggaaaagagg ttgtgaaatc agcggttgtg aaaaagctgt tctgcatcaa gtaataagaa
    22921 cagtcaaaca aaaaatagct cctcatattt ttcgtttggc tggacttatt accgcagatg
    22981 caagcttttg aacaccgttt attcgggaaa gggtttgtga caaaacatgt cacaaaccct
    23041 tttcatcatt tcatagaaag gataaagaat gcaaaaaaaa attagcatca tcactatatt
    23101 ttttatttgt gtgcttactt catgggttat tcagcatcaa gccgctttat atgaagtacc
    23161 tgtcatcgag atagaaaaag tacgaataaa agaggatcat caattgatta caggaaaact
    23221 gatgaacacg gatcaaaaag gagaatcgat ttatttatcg gttccttatc aaaaaaatca
    23281 aatagaaaca gcaaatataa aagcgagaca aatgtatcta gtccaaatgc atggagatga
    23341 atggcagcta gtagcaaaaa aaaatgatgg ctggcttttc ttcttcacaa gtatcttcat
    23401 tggcgccatg ttgcttattg gtggaaaaca gggtctattt gctttaggaa gtctcatcat
    23461 caatatcgtt ttacttttac ttttgttgct catctttact aaaacagaag ggatttctct
    23521 attaaatata tctatcctgt tcgtcgttat agggatgatt attagcatat tggctttaga
    23581 tggatggaat agaagcagta taatcaaaat ttcagctgca gttacgagtg tttttttagc
    23641 attttttatt tgtcttttga caatgaatca ttggaaagac caaggactgc gctatgagga
    23701 actgaattat ctgacacgac cgtatcgttc tgtttttctt tctagcgtac tgattggaac
    23761 agctggagga acactcgata ccgttatcac agtcattgcg acattagaag aattgactaa
    23821 gcgacaacca gaaataacag cgaaacagtt atggctttca ggaaggcaag tgggtcgaga
    23881 tgtgattggc tcgatgacca atatcctttt gttcgtctat atcagtgggt cagttccatc
    23941 gcttctttta tatttaggaa atcaatggtc gttcaaagaa acgatagaac agcatttatc
    24001 attggaattt ttacgcgtca ttgctggcag cttaggaata gtattatcga tccctatctc
    24061 ggtcctcttt tttctgactg taaggaggct gaaacgatga atgtacttat tatacttgtt
    24121 gggattttag caatcagcgt gctacttatt ggcggtacgc aaggtatgta tattctttta
    24181 ggcttattca tcaatttagg gatctttttc ctgttgttat tcggttatca tcagaagtgg
    24241 ccgatactgg cattgtcggt cataggattt ttattgattg ctgtggttat cttatttttt
    24301 atcaacggtt ataatctaaa aatgcgtgca gcttttgtga gtatactgat ctttttattt
    24361 tgtttcctac tattgcttcc tatcactgat tttttagcaa tccaaggttt tacatcggta
    24421 gaactagaag aattatctgg cttagataag actttagcga ttgattttag attacttacg
    24481 agatcgttgc ttctgattag tcttagtggt gctgttttag atgcgagtgt agcaatttct
    24541 agtgggacat tcgaagtata tcaggcaaat ccgcaattat ctttttacca attgcgccat
    24601 tctagtttcg ctattgctaa aaaaatattg gcgtcaacag taatgacact tctttttgca
    24661 ttcatgggaa gttctttagc gttgatcctc tggtttattg atttagacat tccttttcag
    24721 caagtgatca atgaaaaatc atttgttcta gaatatacga tggctattct taccacattg
    24781 tccgcactac ttgttcttcc tttaacctgc gtgactacag catatctctt tacaaaaaag
    24841 aaagaacaac tcaagatgga gtaaaagtta caacttaagt cctatgacat ttttactgaa
    24901 tcagtgcata atatggatgt aaggaaaagc ttgcaaacaa ttttgcttaa ggaggcaatt
    24961 tccatgaaag aaagagaacg tattttagat ttagtgaaaa aaggaatcct ttcgactgaa
    25021 gaaggcttag atttattaga aagcatggca actgaaaaag acgaaaagca aatcaaaaaa
    25081 gaagcagaca aggtaactgc tagtcataaa gaaaaagatc aggcaagtca attgatcgat
    25141 aaacttgaaa acggcgaaga agaaatttct gagccagttg atccaaagga aagagaaaga
    25201 caagatcaag aaaacttaga aaagatctta gatgaactag caactgaagc gaataaaact
    25261 tcggcgcgtc tagatgaagt aaatgctcaa ttcgctgaca ataaggcagc acgcaatgag
    25321 aaacaggaag cattgatgca gctgaataca aaagaagaat taggcgagtt gacagaagaa
    25381 gaattagcac agcgtcagac gttagaagca gaaatcaaag aattagaagc tagtggcgat
    25441 acactgttag aagaacaatt gaaattagaa gcagaattaa aagatatccg taagaatcaa
    25501 tggtctgaga aaaaagaaac gtttactgat aagtttgaat tgccagatga ttggaaagat
    25561 caagcaacag atacactgaa tcaagtaggc gaaaaaatgt cagaagctgg atcacagtta
    25621 ggcaaattct tgaagaaaac attccaaact gtttcagaaa cagtgaatga caacatggaa
    25681 tggaaagatg tcagcttacg tgtccctggg attgcgacaa cgaaatttga gcatgaattt
    25741 tactatgaag ctcctgcagc aagtatcctt gatatcaagg cagcaaacgg aaacgtgacg
    25801 ttaaaaacgt gggacagcaa cgatgtgaaa gttgaagcta agatcaaact ttacggaaaa
    25861 atgggtgctg aaccatttga agcattctct gaacggagcc agattgaggt taatgaagat
    25921 catatctcct tccaaattcc aaataaacgt gtgcgtgcag accttgtttt ctacttacct
    25981 aaacgagtat acgaccacgc agctattaaa cttctaaatg gcaacatcat gattgaaaca
    26041 ttggaagcta aagatattta tacaaaatct acgaatggta acatcatcgt ggaccaatta
    26101 actgcaacga tgctggaagt agaaggtgta aacggcaata tcgatattcg aaacgggaac
    26161 atcttagatt ctattatcga aacagtaaat ggtacagtga catttggtgc gacagcagaa
    26221 aacctttctg tgtctttggt aaatggtgat gtacgtctta cgattaaaga agataatttg
    26281 aaaaaagtag aagcaagttc agtgaacgga aatgtcaaag tcgctttacc agatggcatc
    26341 ggtttggaag gacatgctaa aacaagtctt ggaagtatca acagccgtct gtccaattac
    26401 gaagtagttc gtgagaaaaa agaacggaca aaccaaatgt tgcaattccg tcgtgtttca
    26461 gatggtgaaa tcgcgcaagt gcaactttca acgacaacag gaagtatcta tttaaaagat
    26521 acagataaat aaagaggttg tgaaagagat gttcagcctt gagcaataag aagcgatttt
    26581 gtaaaatagc ttcccctatt ttacaaaatt gtgccttatt gccgatagtc tatctcttga
    26641 acaccgttta ccaagagatt atgaacctgc caattatcgc cgagtcacaa ggaataattt
    26701 cagaaaaagc tcgtcacgtt ttatgaatat ggtaatttgt gaccgagaat tttgcagaaa
    26761 gacaccgatt aagaagaagg ttgtcacaac gattttcttc atttaaaata gtagaacaga
    26821 ggtgagacat gatgaaaaaa aagttgacaa aatctaataa aaatgttgtt ttgacaggga
    26881 ctttagcagg aataggagaa tacatcggga ttgatccgac tgttatccgt gtgctttatg
    26941 tattcctgag cttagttgct tttggaagtc caattatttt atacatttta ttagctttga
    27001 ttatcccatc tgcgcgttct gcacgaacaa actatggaca taacaatgca tattatgaaa
    27061 aaaacaatta tcgtgaacaa actcgtgaac aaacaaaaaa acgcaaagaa gcggaaaaaa
    27121 tcgatgatga cgaatggagt gacttttaat gtcgtacttt caacgtattt tagttaatac
    27181 gttaacattc atatcattgg ctgttctttt tcctgcgatg gtccatgtga gcagtatttg
    27241 gacggccgtt cttgccagct tcgtattgtc tgtgttgaat atgtttgtca aaccgatttt
    27301 gacgatactg tccctgccct ttacattagt cacattggga ttgtttacgt ttgttatcaa
    27361 tgcagcgatt cttaaaatga cctccttttt tgtgggagca gaacatttta gtttttcttc
    27421 attcggtgca gcgttgttag tagctatctt gatgtcgctt gtcaatgtaa ttgtcagcga
    27481 acataacagt agaaaataaa agaaagctgt gccgaactat cggcgcagtt tttttattta
    27541 gaatcaggaa tgcgttttct attacattgg taattaaata aaaacgtttt ttttaattgg
    27601 tagtactttt ggctagacaa tgataaaatg atagagaaca ctctaataga aatgaggtag
    27661 catgaatgtc agaagttgta aaagttagcc aattagtgaa aaaattatct ttggaaatcg
    27721 tgacaggcga cgagaaaagc ttagaccgtg tcatcgtaac tggggacatc tcacgcccag
    27781 gactagagtt gactggttat tttaattatt attcccataa tcgtttacag ctatttggca
    27841 gtaaagaaat cagttttgcg gaacggatga tcccagaaga gcgtttgatc atcatgagaa
    27901 gattatgtag tgaagatatg cccgcattta ttatttcaag aggattacca gctccagaag
    27961 aaatgatcca ggcagcaaat gaaaatggga tagctgtact gcgttctccc atctcaactt
    28021 ctcgtttatt aggagaatta tccagttatt tagatggtcg ccttgcccct cgaacaagtg
    28081 tccacggtgt tttagtggat gtttacggat taggtgtact gattcaaggt gacagtggga
    28141 taggaaaaag tgaaacagcg ttggagttga tcaaacgcgg ccatcgattg atcgcagacg
    28201 atcgagtaga tgtttatcag caagatgaat taacggtgat cggagaaccg cctaaaatcc
    28261 tggaacatct gattgaaatc cgaggaatcg ggatcattga tgtaatgaat ctttttggtg
    28321 cgagtgcggt acgtggctct atgcaagtcc agttggctgt ttatctggaa gcatgggcaa
    28381 aagataagaa atacgaccgt ctgggctctg aagacacgac cgttgaaatc gcggaagtgg
    28441 gcattccaca aataaaaatc cctgtcaaaa caggacgaaa cgttgcaatc atcattgaag
    28501 tagccgcgat gaatttccgc gcacgtacaa tgggctttga cgcaacgaaa acatttgaag
    28561 aaagattaag tcgcttgatt gaagaaaact caggcaacga ctagcaagga gtgacaatat
    28621 gttagcacaa atcaatcgag ttgcttttga gcttgtaggg attcctatct actggtatgc
    28681 attgatcatc gtatcaggta tcattatcgc tatgtggctt agcagcagag aggctgttcg
    28741 ggtaggatta aaagcagatg atgtgacaga ttttatgttg tggggattgc ctatctccat
    28801 tattggcgct cgcttatatt atgttttatt tgatcttccc cagtatatag ctgatccaat
    28861 ccaaatattc aatatccgtt caggaggctt agctatttat ggcggactga ttgcaggcgc
    28921 gatcacgctg tattttttta ctaaatatca ttttatttct ttgtggactt ttttagatat
    28981 cgctgcacca agtgtattat tagcacaggc aatcggtcgt tggggaaatt tcatgaacca
    29041 cgaagcatat ggaccagaca cgacgcgaag cttcttagaa agtctgcatt taccgcaatt
    29101 catcattgat aatatgtata tcgaagggat ctatcgtcaa ccaacatttc tttatgaatc
    29161 actttggagt ttgattggat ttattttgct tttgttttta cgaaagaaac cacacctttt
    29221 gaaaaaaggc gaagtccttt tatgttatct aatgtggtat agtttcgggc gtttctttat
    29281 tgaaggatta cgtacagaca gtctttactt tttaggtgtg atccgtattt ctcaattatt
    29341 atctgctgtg ttatttgtcg gtacattgat tctatttatt tggcgcagaa aaaaaggaaa
    29401 tttaccggat tatgaccgct cattaggaaa aaatcaacaa attatctgat ttttagagcg
    29461 ttaaaaatga aaaaaatttc aggagtggta aaatgaatta tagagtagcg gttttaggac
    29521 caggctcttg gggtactgct ttggcacaag tcctcgccga aaacggacat gacgttcgga
    29581 tttggggaca tcgtgcttca caagtagaag aaatcaacac ccaacacacg aatcaacgtt
    29641 atttaccaga tcattatcta cccgattcca tccaagcatt caccaatatg gaagatgcag
    29701 taaaagacgc agatgcggtg ctattcgtca ttccaacgaa agcaatacgt tctgttgcaa
    29761 aagaactagt gcctaaattg aatacaaagc cagtgatcat ccatgcaagt aaaggattag
    29821 aacagggaac acataaaagg atctctgaca tcttgttaga ggaaatccca gcagaaaaaa
    29881 gaaaagctgt tgttgttctt tctggaccaa gccatgcaga agaagtagca aaacatgaca
    29941 tcactacgat cacagctgca agtacagatg caaaagcagc aaaatatgta caagagctgt
    30001 tcatgaataa ttattttcgg atctatacca atcctgatgt gatcggtgta gagatggggg
    30061 cagctttgaa aaatattatt gctattggtg ctggcgctat tcacggctta gggtttggcg
    30121 ataatgcaaa ggcagcaatc atgacgcgcg gattagctga gatcagccgt ttaggtgtag
    30181 caatgggtgc caacccgctg acctttatcg gattaagcgg aataggcgat ctagtcgtta
    30241 cttgcacaag tgttcattct aggaactggc gcgcaggcaa cttgttagga caagggcagc
    30301 ccctagaaga ggtgctagcc aatatgggaa tggtcgttga aggtgtgaat actacccaag
    30361 cagcgatgga actagcccaa atgatggaaa tcgaaatgcc catcactcaa acaatctatg
    30421 aagtgctgta tgaacaaaaa gaaatcaaaa cagccgcaaa agaaatcatg ttacgtgatg
    30481 gaaaaaaaga aaatgaattc tcattaacaa ttttttgaag gagcgaatgt atatgaaagt
    30541 taaaaaagca gttattccag cagcaggatt aggaacaaga tttttaccag caacaaaagc
    30601 tatggcaaag gaaatgttgc caattgttga taaaccaaca atccagttca tcgttgaaga
    30661 agcgttgaaa tcaggtatcg aagacatctt gatcgttaca ggaaaagcta aacgaccaat
    30721 cgaagatcac tttgattcaa atattgaatt agaaacaaac ctaagtgaaa aaggaaagac
    30781 cgaattattg aaattagtag aagaaacgac agatgtcaac ttgcatttca tccgtcaatc
    30841 tcatccaaag ggtttaggac atgccgttct tcaagcaaga gcctttattg gaaatgaacc
    30901 atttgtcgtt atgctaggcg atgatataat ggaagacgaa gtaccattga cacagcaatt
    30961 aatcgatgat tacgaacgta ctcatgcttc tacgatcgcc gttatgcaag ttcctcatga
    31021 tgaaacttca aaatacggaa ttatcaatcc aggagaagta ttggaagatg ggttatacaa
    31081 tgtcaaaaac tttgttgaaa aaccagatcc aagcaaagca ccaagtgatt tagctattat
    31141 tggacgttac ttattgactc cagaaatttt tgatgtattg tccgagcaac aaccaggagc
    31201 cggaaatgaa atccaactaa ctgacgcaat cgatacattg aataaaacac aacgcgtatt
    31261 tgcccgtaaa ttcacaggta aacgttacga tgtcggtgac aaatttggtt ttatgaaaac
    31321 aagtattgaa tatggtctaa cacatccaga agtaggtgca cctttacgtg agtacatcct
    31381 gtctttaggt aaaacattag aagcagaagg aagcaagatc gatcataatc caaaaggaat
    31441 aacagaaatc aaagaagaaa agaaaaaata accctttttt ctaacgagct tcagctgcta
    31501 attgaataaa aagtagttga agctcgtttt tatttgttta aaaaacacga aaatgctatc
    31561 cttatagaaa agagagacaa gaagggagtt tgatcatgtc aggaggagaa attgcaggat
    31621 tgatcgcagc aatcgccttt gcgatacttg tcgtttttat cgttcgtgtg ttgttgcaag
    31681 tttctaaaac tttagaaaaa gtagatcaga ctgtagcaga agcaaatacg acgatcgaaa
    31741 tcgtcacaaa agacgtagat cttttgtcta gacaagtaga aggcttactg gtcaaaagta
    31801 atgaactgct cttggatatc aatggaaaag tcgcaacgat cgatccattg ttcacagcag
    31861 ttgctgattt aagtgaaagt gtatcagaac ttaatcattc tagtaaaaac atcgcgacga
    31921 aagtcggcgg aatcggtaaa acgacagcta aagcgacggt tgccggaaaa gtcggtggga
    31981 cagcgatgaa attttttaaa aacaaaaaac atactgaaac gacaggagga aaataaaaat
    32041 ggctaaaaaa ggtggatttt tattaggtgc agtagtcgga ggcacagccg cagcaatcgc
    32101 cgcgttattg ttagcaccaa aatctggtaa agaacttcgg gatgaattag ctgcccaagc
    32161 ggatgatttg atggatatgg cttcagatta cacagatatg gcgaaagaaa aatcgactga
    32221 gttaacaggt attgcaaaag aaaaagcgca agatctttct caacaagcag gtgatctagc
    32281 aggtagtgtc aaacaaaaag taagtgaatc agcagatgaa gcaggaagtg aatctgacga
    32341 tatcttgtta aatcttaaag aacaatcagc tgacttatct aatcgtttca aaaaaacggc
    32401 agaagatatg aaaaaacctg cagatgaatt aggaagtatt gctaaagata cttcagaaga
    32461 tatcatgatt gatgtaaaag acgctgctgg taaagcagga gatactgtca atgaaggaat
    32521 gtcagaagca aaaccagttg cagatgactc aaaagaaatg atgaaaatgg caaaagaaga
    32581 tgcgaaagaa gctaaaaaag aaataaaaga tacaatgaca gattcatcta caaatcacta
    32641 aaatcacaaa tttatagaat aaaaaggtgg gagcggacta gctctcacct tttttactct
    32701 tatttttcta aaacaagtaa ttcttttggt gtagtcgtga atggcacaca gccatcgctt
    32761 gtcacatata cacagtcttc gatacgtacc cctacttttt caggaatata gattcctggt
    32821 tcaagagaga agcacatccc ttcttcgatt actaagtcat ttccttctac aagggaaggg
    32881 aattcgtgga cggttgttcc gatcccgtga cctaaacggt gattgaaata ttcgccgtag
    32941 ccatagctgt tgatcacgtt tcgtgcgatt tgatcaagtt cacctgctgt cacacctgga
    33001 cggacggctt cttgtgcggc aagctgtgct tcaagagtga tgttgtagat tttttcttgg
    33061 aagtcggttg gtttttgata agcaattgta cgagtagcat cactgcaata gccattccaa
    33121 acaacgccaa gatcgaataa cacaagttca ttcggtgaaa tcttcgtatt tcctggtgta
    33181 ccatgagggc ttgcagcatt tgctcctgca aggactaatg tgtcgaaaga catttgtgaa
    33241 acaccttgtt ttttcaattg gtattcaatt tctgcaatga tcgcttgttc agtcacgcct
    33301 tctttcactg ctttaaagcc aatctcaaat gcaacatcgg cccatttacc agcttcgatc
    33361 agtttgtctt tttcctctag agttttgatc agttgtagac gttggatgat ggaagttaga
    33421 tcagtgtaag aatccgttcc cgtcaagata tcagagatag catcgaagcg ctcgacagtc
    33481 aaggcgttct tttcaatccc tacattgcca ggctgaccat agcgagcacg aagttctttt
    33541 ccgatgatct cccaagggtt ttcgctgtct aaatagccac ggacatcata cgcccaacta
    33601 ctgttttgag catcttcaac ttccaaagca ggagtgaaaa ggaaaggatc ttgttcaaga
    33661 gggataaaaa gagctagtac acgctcatga ggatcacttt cgtatccgga aaagtaagcg
    33721 atgtggccag ggtcgctgat ataagccaat tcgacttggt gttgtgccat ccattgacgt
    33781 agttcattga tttttacgtt attcatttgg caacctactt tctaaattaa ttttgtattc
    33841 aataattgta ccactgattt attaaaagac caacgttcta aaaactgaaa aaagtaaaaa
    33901 ttttcatgaa aacagagata aaacggttgc aattcttgag attatttgat attctatttt
    33961 agaaatgtaa gacgtatttt cagaaaacat ataaaatatg aataggagaa cacaaatgga
    34021 aaagcaaaca atcactatct atgatgtagc gagagaggca aatgtttcga tggctactgt
    34081 ttcacgtgtc gtaaatggca atccaaacgt taaaccagca acaagaaaaa aagtcttgga
    34141 agttatcgat cgcttggatt atcgtccaaa cgcagttgcg cgtggcttgg caagtaagaa
    34201 aacaacaaca gttggtgtaa tcatccctga tgtcagcaat atgttctttg cttctttagc
    34261 acgtggtatc gatgatgtgg caacaatgta caaatacaat attatcttag caaattcaga
    34321 tggcaacgac caaaaagaag tcaatgtatt gaataactta ctggctaagc aagtggacgg
    34381 cgtgatcttc atgggtcacc atatcacaga cgaaatccgt ggagaatttt cacgttcaaa
    34441 aactcctgtt gtattagctg gctctattga tccagatgaa caagtaggta gcgtaaacat
    34501 tgattatgta tccgcaacaa aagatgcagt aaacaaattg gcaaaaagcg gcaataaaaa
    34561 aatcgctttt gtcagtggtg cgttgatcga tcccatcaat ggacaaaatc gtctgaaagg
    34621 ctataaagaa gcattaagcg aaaataacct accatatagc gaaggattga tctttgaagc
    34681 acaatacaac ttcaaagatg gtctatctct agtcgatcgg atccataata gcggtgcaac
    34741 agctgtctat gtttcagatg atgaattagc catcggtatt ttggatggtt tattagaccg
    34801 tggcgtgaaa gtaccggaag aattcgaaat catcacaagt aacaattcac tactgacaga
    34861 agtagctcgt cctcgtttaa caagtatcac acaaccatta tacgatttag gggcggtatc
    34921 tatgcgccta ttgacgaaaa tgatgaataa agaagaagtc gaagaaaaaa cgattatctt
    34981 gccttatgga atcgaagaaa aaggatctac aaaataataa aaggacaacc attcagaggt
    35041 tgtgtgcctg ccgtttaact ctgcgttaca aggagcaatt tcaaaaaaca gcatctcatg
    35101 tttgatgaat attgtgactc gtgattcgga gttggcagga gaacaccgtt tatttgggaa
    35161 aaggggttat gacaaaattt tgttataatc ctttttttgt gtcttaaaaa agagagaaag
    35221 tggttgaaat tacatttatc gtatataaaa caatccgtgt atagatgctg cattttgttc
    35281 accaccatca aaaaaataag ggactgtgac aatactattg tcacaatccc ttattactgt
    35341 gtattctatt aaatcaatca tctttctcat ctgattcttt agttgcttga ttcgctcttg
    35401 cataagctga agcggtgttc cagtaatctg tatagttttc atcggtaccg ccaacgccaa
    35461 atttaaaggc gcttttcttc ggtccatttt tcgcccatag cgattcaact gtttttcctg
    35521 gtgtttggat ggatttttta ttgacagtaa cttttccagg tgtttgacca gtaaatccag
    35581 atactttttt cttcatcaca tcagatgata attcgaattt ctcatcagtg ccaaaaatgt
    35641 caggttttgc ttggtaagct tgattgatca gattggcaag ataattagcc gaacgtatcc
    35701 ttgctttgct gtccattcca gtattgtcat cgtgccctgc ccagctactg atggtaatag
    35761 acggagtaga gacaattagc catgaatctc ggtattcatt ggtagaacca gtttttccga
    35821 cccaatctgc ttttcctaga tttccgttta agcttgagat ggcatctttg aatggtgtag
    35881 tgatttttgc attgatcaca ctacgcatca tgtcattcat gatcgatgcc gttgcttccg
    35941 aatagatccg gacagagttg ctttcgtgtt tataaattac atttcctgcg ttatctgtga
    36001 tggaatcgat gatatagcct tcttcataga cgccgccgtt tgctaaagcc tgaaatccgt
    36061 tggtctgttg aagcgtcgtg acatctactg tacctaaagg agcagattca acgccccaat
    36121 tgtcatttgc cgggtagttc atctttgcca agtagtgctc gtaagcatat tgtttcgatc
    36181 caccggaatt caacacatct tgatataagt gataagccgg gatattgttg gaccattcta
    36241 gtgactcacg gacggtttgg aaggtgttcg atccttcgtt agtagcattg acgattttct
    36301 cgccagcatt ttctccttct tgccaagttg ttgcataatc tgatacacgg gattctgatc
    36361 cgataagccc catgtcgatt gcaggtccat aaacaagaac tggtttgatt gatgagccag
    36421 cttgacgttc tgtatcgaag gcatggttat tttgattctg actgtaattc cgtccgccaa
    36481 cgaatccgta gatgcggcca gttctgttat ccatcaatac atttccggtt tctacttgac
    36541 tgccagcacc atcatctaac atgtatccat aattttcaac gccattttgc aatgcagcat
    36601 agacatcttt atcgattgtc gaatggattg tatatccttt attttggatc gtttgctgtg
    36661 ccttttgata ataagcgtca ctgaccgatg aatcagacat atcagcgttg tctttttcag
    36721 ccaattgttg tgcaatgatt cttgttgctt cattcatcac agtataatac aagaattctc
    36781 tgtcagtctg ttctgcgatt tgttgtggta gaaagtcttt tgtaaggtca taggcttttg
    36841 cttcttcgta ttgttctttc gtaatttggc cttcacgata catactgaat aaaacaaaat
    36901 cttttctggc aaggccggca gaaagatctt cttttagcgc gcctgtgttc gtgtatggac
    36961 tatacgtgat cgggctttgg ggtaatccag cgatataagc agcttgcggc agcgtcacat
    37021 cttttgcatc tacaccaaag attccttttg cagcttcctg gactccagca atgttttgtc
    37081 ccttattatt acgaccaaat ggtgagacat tcaaatacgt agcaatgatc tcatcttttg
    37141 aaaagtattt ttctacttgt gcagaaagaa tgatctcgtt tgctttacgt ttgaatgttg
    37201 tttcatctgt aaggatctgc tgtttgacta attgctgtgt caaagttgat ccaccagaag
    37261 agccaatacc agtagcttca gatactaatg ctcgtaaaac agctttagga acgtaacctt
    37321 ggtgtttatc gaagtattca tcctctgtgc tgataattgc agttttcaat aaaggtgaaa
    37381 tcttatcaga tgaaatagtc gtgcgcatca gatctgtctg gatttttgat atctctgtat
    37441 tatctgcata aacaatcttt gacgtttctg taatgtttcc tagttctgtc tgcagtgctt
    37501 ttttagtcgg caattgtgtg tcttcgacga gataggcaaa atagccagcg cctattccaa
    37561 tacctagagc tgcgaacatc aaaagaagaa cagtaataaa aacagtcaat gattgaaaaa
    37621 ctctaaaaat aatcttagga acaaaccatt tttctttttt tcttctattc tgtttttttc
    37681 gagtattttt gcgtggcaaa agggtcacct caactaatgg ttttatttcg ctaattatag
    37741 caaataatag aacaaaaaaa cagacaaggc tgtttttggc aggaatttct tttttaaaag
    37801 agttaaaacg tgagaaaatg atctttatta taaaagagtt cttaagcttt tctttagaga
    37861 aaatttcagt tactatggag aacagggcaa agaaaaatca atcattttct ttgtatatca
    37921 tcttaccaat atataggaat tgttagtaac aattgccttt caaggaggaa tccatccatg
    37981 ttattaggct cacacgtcag catgagcgga aagaaaatgc tgttaggagc agcagaagaa
    38041 gcagccagtt atggatcttc gacttttatg atctatacag gcgcccctca aaacactaga
    38101 agaaaagcca tcgaagagat gaatattgat gccggaaaag cgtacatggc agaaaaaggt
    38161 ctaacaaata tcgtcgttca tgccccttat atcattaatt tggggaatac gatcaaacca
    38221 gaaatgtttc cttttgccgt ccaatttctg agagaagaga ttgttcgtgc ggaagcttta
    38281 ggcgctactc aaattacact tcacccaggt gctcatgtag gggcaggaac agaagccgga
    38341 attgctagaa ttattgaagg cttgaatgaa gttttgacaa aagaacagac ggcacaaatc
    38401 gctttggaga caatggcagg taaaggtacg gaaatcggtc gaacatttga ggaattagcg
    38461 caaatcatcg atggagtaaa attgaatgaa aaattatccg ttacttttga cacctgtcat
    38521 acgaatgatg ccggttataa tattaaagaa gacttttcag gtgtaatgga agagtttgat
    38581 aagatcatcg ggttagatcg gcttaaagta ctccatatca atgattctaa aaatccgcaa
    38641 ggttcgcata aagaccggca cgccaatatt ggttttggaa caatcggatt cgacacgctg
    38701 aataagatcg tccatgatcc aatgtttgac aatatttcga aaatcttgga aactccctat
    38761 gtaggcaaag ataaaaagaa agcttacgca ccatatggaa aagaaatcgc catgttcaaa
    38821 gcgcaaacat tcgatccaga agtgtttcct gagttattag cagaagaagt gatactctaa
    38881 gattttctat ctgacagcag caaaaaagga ccaggcacat tgccttgtcc ttttttgttt
    38941 aagaaacaaa ggagtacttt ttgcagtctt tttcatcatg catcttctcc cccctctttt
    39001 taaatttaga caaacaaggt atactggtag aaaagtaaaa aaacgaaaag tttcacaagc
    39061 aagtggcaaa acgtgattgg aaggacgaaa agatgctgaa cactttaaaa cccttgatca
    39121 accaactaaa aaagaaacat acatacttta ttcttggcat agccctatta tttacaatta
    39181 ttcgtttgta tttatatagt aaagcaatct atggaacaga catgtcggca acctatgatg
    39241 accagctgct gattcgctat gctcaaaata ttttggacgg taaatggcta ggggagtata
    39301 cgactacaac actttccaaa ggaatctcct acagcttatt tttagtactt gcacataaac
    39361 tgcatctttc ttatgcagtt ttgttcggca tgttcagtat ttttgccagt atattggttg
    39421 cactcgcgtt taaaccgatt gtgaagaaca agtatcttct tttaagtatc tatttttatt
    39481 ttttgttttc tccgattagt tttacacatg aatattccac tcgaacatat cgtaatgccc
    39541 tcgtgattcc ggctgttgag atcattatcg cttgtttatt agctatttat taccgcaaat
    39601 acggttctag cagaaaattg ctgccatggt ttttagggct ttctgtcgct tttcctttct
    39661 tttggttcat tcgcgaagat tctatctggc ttcttccttt tactgtagta gctttgggta
    39721 tcagtttcgt ccagatcttg ctagggcaag acttttcttt caaagaagta aagaagttaa
    39781 aaaaacgact ctttcttctc tcgaaacggg aatggctgaa actttcattg tttttacttc
    39841 ctatagttgg cttttccatc gtctatcaat ggatcgaata taaaaatgag acaacatacg
    39901 gtattgcaac catcaatgac cgttccagtg gagaatttgg acgtttgatg aaacaactga
    39961 tccgaataga tgatgaaacg aacttgaata aagaaaacag tgagatatgg gtctcacatg
    40021 ctgcactaaa taaagcatta gccgcatctc ctacattcca tactgtttct acccgtattg
    40081 acgaattata tacgactcac gcgtggactc aaggtggaaa agtaaaagaa ttacccggag
    40141 atatcatctt ttgggcattg cgagatgtgg taagcgaatc tggttattac caaaacaatg
    40201 caaaagaaac caatgatttt tggaaaaaag tcaaccaaga attagaagaa gcctatcaca
    40261 agggaacaat caaaaagaaa aaagagttct atctgatggg atcaggagat ggaaagacga
    40321 tgagtgatct gccaatcgta ttcgatttcc ttcaatcagg atacgcttat gcatggcgat
    40381 ataaagattt ccatcaagga ggaaattata gtttcggaac gcaagaacaa gtcaaacagg
    40441 ctcaagaatt attaaacctt ccgctaatgg agaattggat cgatagtaat gaaccgagaa
    40501 cacctaattt ccaattaaca aaatcagcaa aagtcgcgaa tgtcatcata aaaatttacc
    40561 aagttctggc tattcccgtg ctgattgctt caatagcagg gatattactt atgatactag
    40621 gaaccgtatt tgcaaaagct gatcgtgcct attatagttc ctctttgatt gtggtcaccg
    40681 gattaatact gacacaattg gtttttcttt tcggtgtctc atggttttgt tcttatgcgc
    40741 cagaaaaaaa ggactatttt ttaagtgtct acactggtgc aggcgtgcca ttaacgcaat
    40801 tcatcgtagt aacaagcatg ctagttttca gtaaattaaa tctgaaaaaa gctttatctt
    40861 ctaaataaaa agacccttgg tagctagatt tttaacctaa ctatcaaggg tctttgtgtg
    40921 gaaaaatcag tttttattcg atttcttcta ccgtttcctg caaaagctgt tcatattttt
    40981 tgtttccttt atataaatca ccaatcgtcc atgccaacag gagaaggaca agaacaatga
    41041 ttacataagc gatgtgatca gccaatgcta gttgagcgga agtggcttgc tcgccgaaga
    41101 aactttcgat ctgtcctggt aatcctcgta agttcaagta agttagacta atcacagaga
    41161 accagccaag aagttttacg atgaatgaat ttttaaatcg gctacccatc tccgcttggc
    41221 tgttggtcat catcagtaaa ggaatcatcg aaaacggcaa cgcaaaagcc aagaacacct
    41281 gggattcatt cattaagtta ttcaaagcgg catgttcttc gatcgttcct tttgtacttg
    41341 taaacaaaac acaaatcaat acaggaatca cagaaagcaa tcgtgtgacc agtcttctta
    41401 gccaaatcgg gatacgcata tggatgaatc cttccatgat gacttgaccg gtcaacgtac
    41461 cagtaatagt agagttctgg ccagaagcaa gaagtgctac agcaaataaa gtggacaaga
    41521 taccagattt tgctacagca atcaaaatac cattgctcat agctgatgga tcagataaag
    41581 cttcatacag accaaaaaag gaaggatctt ggacagaacc ggttttgaag acagcgacac
    41641 ccatgatcaa tagcagtgca ttgactagaa aagccattga caactggata ttagaatccc
    41701 aagtagaaaa acgtacagct tgtgctacat cttcttcgtt ttggtgatcg atcttccgag
    41761 tttgtgaaac tgctgaatga agatataagt tatgcggcat gactgttgca ccaataatcc
    41821 ctaaagctcc agtcagcgga gtttcgccgc cgatgaccgg tgagtctgag aatgtatgac
    41881 tgttagggat aaagccagca aataaactgt gccaatctgg gttagacaag ccaacttgat
    41941 agacaaaaac gaataaaatg acgaaaatta agcagacaac cagtgcttcg atcttacgaa
    42001 acccgacttt tgtcagtagt aagagaacga aaacatctaa tacagtaata aagacggcaa
    42061 tcaccaatgg aatatcaaaa agtaagtaga gggcgattgc tgcaccaatg acttctgcaa
    42121 tatctgttgc cataatggct aattcagtca agatccataa aatgacacct aatgtcttac
    42181 ttgtgcgtgc tcggattgcc tgtgccaaat ccatttggct cacgattcct aattttgctg
    42241 ccatatactg taaaagcata gcaatcagac tagacatcaa aataactgac atcaataaat
    42301 attggaaatt ttgtcctccg gtgatcgaag tagaccagtt acccgggtcc atatacccaa
    42361 ctgctactaa cgcaccaggt ccagagtaag caagaagtgt tttaaaaaaa cttttcccct
    42421 ttggtacatc aactgttcca tttacttcag ctaaagataa accatttgta tgttcaataa
    42481 gtcgatgctt atgaaattgt ttattggatt gattattcaa gaaaaaacca cctttcctat
    42541 ttatctacga ttacattcta cacctcaaaa agaaaaaatt aaagaaaaag attcggtacg
    42601 cctaattttt tttcatggcg cgtcttgcta gatgacgaaa gaaattttgt tctcctctaa
    42661 aaaaaccact attttttgca tgaaactaaa gaatacgcct ttcttttttc aataaacttt
    42721 agtaaaataa gtcattgaga gcaattcgga aatagaaagg agagatttat atgttatcaa
    42781 caggtattgt cgtacttatt gctattattg ctttgctaat cggcgcagta ggtggctttt
    42841 tcctagcgcg taaatatatg aaagactatt tagaaaaaaa tcctcccgtt aatgaggaca
    42901 tgttgcgatc aatgatgatg tcaatggggc aaaaaccatc tgagaagaaa attcgccaga
    42961 tgatgcagca aatgaagaac caagggaaaa aataggttct gacccaacga ctttttacgg
    43021 gctgattttt tcagtccgtt ttttatttcg taatttgtta gactattcag attattcagc
    43081 aaaaaagaca agcacagaca atcctaggct cttgataaag acaaaggagg agtaaaatgt
    43141 cgatttttag aaaattaggc tggttttttc gtcaggaaaa gaaacattat ttaattgggg
    43201 ttttctcgct attcgcagtg gccttggtac agttagttcc tccaaaagtg attgggatca
    43261 tcattgatga gatcgccgat aaaaatttat caataaagat cattttcggg tggatcttcc
    43321 tattgattat cgctgctttt ttacaatatc tttttcgtta tatctggcgg acgaatattt
    43381 ggggaagtgc agctcgattg gagaaagaac ttcggcagca gttattcaaa cattttacag
    43441 aaatggacaa tctcttttac caaaaatatc ggactgggga tttgatggct cacgcaacga
    43501 atgatttgaa tgcgatccag aatgtagcag gagctggtat cttgaccttt gcagattcag
    43561 tgattacggg cggaacaacg atcattgcga tgattttgtt tattgattgg cgcttaacac
    43621 tgattgcgct gattcctctg cctttgctgg ccgtcacttc acgagtgctg gggtcaaaac
    43681 tccatgatgc ttttcgtgat tcgcaagaag ctttttctag tatcaatgat aagacacaag
    43741 aaagtatcac tgggatcaaa gtcattaaaa catttggaca agaaaaagaa gatctagcgg
    43801 attttgaaga aaagattgaa gatgcaatta ttaaaaacaa acgagtcaat tttttagact
    43861 ccttatttga tccattcatt accttgatta tcggtctgtc ttatgttgca acaattattt
    43921 taggtggaaa gtatgtcttg gaaggcacga tctcgatcgg gcagatggtc tcatttatta
    43981 gttatatcgg tatgcttgtt tggccgatgt ttgcgattgg tcgattgttc aacgtattgg
    44041 aaagaggaaa tgcttcttat gatcgcgtca atgaactttt gcatgaaaag acgcatatcg
    44101 tggaacaact gggtgcagtg cagaccccgg caaaaggcga gctgaatgta gctatttcct
    44161 cttttaatta tccgaaagaa gaatcaccag cattagaaca aatcaagttt gcgatccaag
    44221 aaggagaaac attaggtatt gttgggaaaa ctggtgcggg aaaaacgacg atcatcaaat
    44281 tactgatgcg tgaatacgat gattatcaag ggaaaatcca atttggcgga cgttcgatca
    44341 aagaatattc attgaatgct ttattaggat caatcggcta tgtgcctcag gatcacttct
    44401 tgttttcgat gaccattcga gataatatcc gtttttctaa tccaaaactt tcagaagaag
    44461 aagtagagca tgcggcttct ttagcattta tcaacaatga aatcaaggaa ttaccaaaag
    44521 gttatgatac cttagttgga gaacgtggag tctctttatc tggcggacag aaacaaagga
    44581 tttcgattgc tcgagcattg atcaaaaatc ctgaattatt aatattagat gatgcactct
    44641 ctgcagtaga tgccaaaaca gaagaagcta tcttgcataa tttaaaagaa ataagaaaag
    44701 agaaaacaac tatcattgca gctcaccgtt taagtagcgt gatgcatgca aaagaaatca
    44761 ttgttgtcga tcaagggaaa atcattgaac gaggaaccca tcatgagctg cttgcactag
    44821 gcggctggta tgaacaaatg tgggagaaac agcaattaga agcgaagata gaaggaggcg
    44881 aggcctgatg gatgaaaatt atcaatcgga atggacaaaa tcgattccac ttaaagaaca
    44941 gaaaaagatc atcaaacgac tgatgaagtt cgccaaacct tttagaagaa cgttcatcgt
    45001 tgctatctta tttgcgtttg ctttgtctgt gatcaatgtc ctattgccaa ggatcatcca
    45061 aacttttatg gatgatcatc tggctaaaca gtcggctacg acacaagtta ttttgttttt
    45121 tgctggtatg tacttatttg gagtcatagt aaaaagtatc atttggtttt tccaatggta
    45181 tttgtattcc atggcctcat tgaagaccta tcagtatatt agggtaaaat tatttgaaaa
    45241 gctgcataca ctaggtatgc gttattttga tcagacaccg gcaggatcaa ccgtttctcg
    45301 agtgacgaat gacacagaaa cgctatttga attttggtac gtctttttga tggtattgac
    45361 tgggatcttt gcagtcgttt cttcattcct tgccatgttt caaatcaatg gagaaattgc
    45421 cttgtggact ttggtgtttt tgccgatttt aggaatcgtc atctggtatt accagaaatt
    45481 cagttctcgt atttaccgaa atatgcggga aaaattaagt cagctgaaca cgaaattgaa
    45541 tgaatcgatt tccggcatgc gtattatcca gcagtttcgc caagaagctc gtttagctaa
    45601 agaatttgaa caagtgaaca atgagtattt agacacgcgt tatgcgatga tcaaaacgaa
    45661 ttcgattctg ttaagtccga tcatcaattt tctatatgca ttggcgattg cgttggtgtt
    45721 gactttgttt gggatcgatg cgttgcattc tcccgtagaa gttggactga tctatgcatt
    45781 tacgacctat gtccagggat tttttaatcc aatgtctcaa atgatggact ttttgagcat
    45841 cttcacagat gggatggttg ctggtagtcg tatcttgaag attatggata cggaagaata
    45901 tgcaccacaa caaaaccctg gagcagatca aatgattact tcaggtaaga ttgagtttcg
    45961 caatgtaagc ttttcttatg atggagagca caatgtatta aacaatatct cttttgttgc
    46021 taatccggga gagaccgttg cattagtggg acacaccgga agcggaaaaa gctcgatcat
    46081 caacgtattg atgcggtttt acgaatttta tgaaggtcag atattgattg atgatcatga
    46141 cattcgagaa tatccaatgg aagagttacg taagaaaatg gggctggtct tgcaagatgc
    46201 tttcttgttt tatggggata tcgcaggaaa tattcggatg atgaatccag ctatcacaga
    46261 cgaacagata cgccatgcgg ctgagtttgt tcaagcaaac aaattcatcg atgaattacc
    46321 aaaaggatat cactcaaaag tgatcgaaca tggtgccagc tattctagtg gacaacgaca
    46381 gctgatcact tttgcgcgaa caatcgtgac tgatcctaaa attctagtat tggatgaagc
    46441 aacagcaaat atcgatactg aaacagaagg attgatccaa gaaggattgg caaaaatgcg
    46501 tcaaggacgg acaacgattg cgattgccca tcggctgtca acgattcgag atgcagaatt
    46561 gattttagtt ttagacaaag gtcatatagt agaacgcggg aatcatgaaa cacttctagc
    46621 gcaaaatggt ttgtatgcag atatgtacaa actgcaaagc agtggtatat aaaagatgtt
    46681 gtgacagtag aattttgacc taagcactag agtgaaaata aaacatagct tctcatattt
    46741 ttcatctgtg agagttagtg tcgtagtcac cagatttaaa aaaagattcg acagctagtc
    46801 agaagtgcct aatagccact aagaaagcgt tgacaaaagc atttgctttc gataatataa
    46861 agaaaacgat acaacttgga tagtgattgc ctgatgcaag ctaaacctga tgagtctccc
    46921 atggtttcat ggacggcttt ttgggttttt ctttttgttt tggatacagc cctatacatc
    46981 atggaaagga gcaaaacatg ctgattgaga aagtactgaa taacaacgtc gtaataacca
    47041 gggatgaaag aaatcaggaa atgatcgtga tgggaaaagg tttggcattc aaaaagaaag
    47101 tgggtgaccc gatttttgac tcatgcatcg acaaaaaatt tcagttgaca aatgacgggc
    47161 tttcacaaca gttccaagaa ttggcacaag cgattcctct tgagtactta gaaatttctt
    47221 gtgaaatcat tgaatataca aaagaggctc tacaagtcca gttgaatgat tctgtgtata
    47281 ttgcattgac ggatcatttg tatacggcta tcgaacgtca caaaatggga atatttgtac
    47341 caaatgtatt gctgtatgat atcaaacaac tatttcctaa agagtatgcc atcgggaaaa
    47401 aaacagtgaa gaaaatctcg gataaatacc acatcaagtt atcagaagat gaagccggat
    47461 ttatcgcatt gcatttagtc aatgcacaag gcattcaaag ccagcaagac aatacctacc
    47521 tgtttactat aacaatacag gatattctaa atatcgtccg ctattactat aagattacat
    47581 ttgatgaaga ttcagtccat ttttttcgat tcgtcacaca cttacgtttt tttctgacac
    47641 gcgtcctaga tggagaaaaa tcagaagtgg gagaagtttc tgaggaattg tttcaggtta
    47701 ttcaaaaaaa atataaggat acgtttggtt gcgttgagaa aatagcagac tttttagccg
    47761 agacgttcaa ctaccagatg tccacggacg aaaaaatgta tttaacgatc catactgcgc
    47821 gtttagtgca tgagaatcgt tgtaaataaa aaaaggagga taaattaaca agaacgtatg
    47881 aaataagggt agtgacattc agttggctaa accttccact aataaattgg agggaataga
    47941 aatgggaaaa taccaagaat tagcgagaaa aatcgtagaa aatgtcggcg ggaaggaaaa
    48001 tatcaatggt ttgacacatt gtatcacaag gcttcgtttc aaattaaaaa acgaagaaaa
    48061 agcaaatgat gagatcttga aaaatatgga cggtattgta acggtaatgc gagcgggagg
    48121 gcagtatcaa gttgtcattg ggaatcatgt gccagtcgtg tttgaagagg ttataaaagc
    48181 aggaaacctt acttttgatg aggctgtttc aacagaaaaa atgagaccgt ttgatatgct
    48241 tattgatgga atatcgggct gtttccagcc attccttgct atcctagctg ctggcgggat
    48301 gattcgtgga ctgacagcat tccttgtctt cttaggcgcg tttgaccgcg gttctggcac
    48361 atttgttatg tttgataata tcggtgattc tgtattccaa tttatgcctg ttattatcgg
    48421 tttaacagct gcccgtaaat ttaaagtaaa tgaattcgta gggatgttgc ttggcgcagc
    48481 attaatgaat cctagcttgt cgctggaagc tttgagtggt gcagcagaag caccactaac
    48541 gaccatattt tctggcacta tttttgaagc acctatttat caaacagtat tcggcattcc
    48601 ctggattgcg cgtaactacg cttcttctgt cattcccatt atctttattg tgttgttagc
    48661 tagccaaatt cagaaaccaa ttaaaaagtt agtacctgag atgatcgcga atttcttcgt
    48721 tccattcttc accgttctta ttacaatgcc tttaggtttt ttactcgttg gaccagtatt
    48781 cacattcgca actgatattt taatggctgg atttgaaaca cttttagcac tttcgccagt
    48841 tatttatgga gcaatcgtag gcttcttctg gcaaattctg gtgatgtttg gtctccattg
    48901 ggcaatcgta ccaatgggat tgatgcaatt ttctgtgaat ggttggcaaa atattatgac
    48961 acctgttgca gtagtgtctt ttggacaaac tgcagcactt accgcactct actttaagct
    49021 tagaaatcca aaagacaaag caattgcaat accagccatt gtttctggta ttgtggggat
    49081 tacagagcca gctatttatg ggtttacatt accacgtaaa aaaatattta tttttacctg
    49141 tgtcggtggt gcgttagggg gcgcgtatag tgggttaatg aatttaactt cttggaatca
    49201 aggtggacta ggcatcttta caattcccaa ctatattaga ccagatggtg atttaacaga
    49261 tgtgattaat gtattgattg ggatcgccat tgcgatggtg gtatccttta cgctgacttt
    49321 tttcttttgg aaggatgaag caggtgaaac agatgatata cagaaaaaat caggtaaaga
    49381 aattgtaaaa acaccgatac aaggacaaat cgctccacta aatgcagcaa aggatgcagc
    49441 ttttgcacaa ggaacactag gtcgaggaat cttgatctac cctgaaaaag gagaagtacg
    49501 tgcccctttt gacggaacag tcatgaccct gttcccgacg aaacaggcaa ttggaatggt
    49561 ttctgaaaca gggctggaat tgctgattca tgtcggattg gatactgtgc agttggaagg
    49621 gaaatatttt gaaagcttag tgcagcaagg ataaagagtg aaacaaggcg atctgttgct
    49681 acgcttcgac aaaaagaaaa tcgaaaagga aggatacagt ttagaaacac ctgtgatcgt
    49741 caccaattac acggattatt tggatatttt ggaaaaccaa ggggagacgg tcgggccaga
    49801 cgatgcactt atcaccgcac tgacctgaac ggttaggtga agaatggact aggcttaact
    49861 attcttttta ttccacttct ttattttcaa aattgaaagg ctctttcatg atggctgagc
    49921 tgatagggcg tttatcgaat ttttgaccga cagaaattag atggactaaa ttctgttgat
    49981 aattttctgc aaaaacacaa ctatataaca cgtctagaag ataacaaatt gatgtattga
    50041 tcgtaaaatt accaattttt gaatacagtc gctcgcgtgt cgtaagtcgc aaagcggctg
    50101 tactcattgc agatagcgtt gaatcaccga tactggtcaa agctaaaatt ggaacatgat
    50161 tttgtttcaa tatttctgcg gtacgaagga tcacattgtt ttcacccgta taagaaatca
    50221 gaatcccgca catatcactc gtgcaattgt acgcttcata gaatgattca ccaaaagtgg
    50281 tgacaatcgt gacatccatt ttgatgcgct tcattttcag catgaaatct tgagaaatca
    50341 aagtattcga gttactggcg aaaatcacga ttttttttgc ttgaagcatt agttgtttgg
    50401 ctttttgcag ttggtcatga tgaatgagag aaagtgtatc atgaatcgtc atctgttcta
    50461 aagaagccat tttatttgcg atcgtcataa taccgtctgt ggtggaaaaa ggaagattcg
    50521 catctacgtc ttgaaaattc ccttcaagat aggctgtttc agctaaaaaa gcattacgaa
    50581 aactgaccca gccatcaaaa cccatttttt ttgctacacg gatgagtgta gaggggtgga
    50641 cataacatcg ctcagcaatc gcttggatgg tcatttcgcc aagtgctgga gaatcgctta
    50701 agagaaactg gatcacttca ttttctgaag gagaaaagga gatttctttg attttttcac
    50761 gtaataacat gtagatacct tcttcttatt tttttacgta aattataact tttttgaaaa
    50821 tgatttgtaa acggatttat actatataac ttaaaataga cattttttga caagatattt
    50881 acaaaaatcc actagaaaac agtaaatttt gtaaatgtta ctggaaagaa acagatttct
    50941 attgtttgtt ttgtttacaa aaaagataat gaaagtgtaa aaaagtaaag gagtgagaac
    51001 atggcaaaag gaatcaaaat cgctacgatt ggtggaggca gcagttacac ccccgagttg
    51061 atggaaggat ttatcaaaag atatgaggaa ttaccgatac gagaaatctg gctcgtagat
    51121 gtagaagaag gaaaagaaaa gcttgagatc gttagtcaga tgtctcagcg tatgtgggat
    51181 gcttctccgt atgaagtcaa gatctatgca actctggatc gggaagaagc attgaaagat
    51241 gcggattttg ttacgactca gtttcgtgtc ggtcaattag atgcacgaat caaagacgag
    51301 cggatcccta gttattatgg gatgattggg caagaaacaa atggtgctgg cgggatattc
    51361 aaagcttttc gtaccgttcc agtcgttttg gcaatcgttg aggacatgaa gcgtttatgt
    51421 ccaaatgctt ggctgatcaa tttcgcgaac ccaagtggga tggtgacaga agcagttatc
    51481 agatatggca aatgggataa agtgattgga ttatgcaatg tgccagtgat ggcgatgatg
    51541 acagaaccag caatgcttgg caagaaaaaa gaagagctga tctacaagtt tgccggtcta
    51601 aatcatttcc attggcacaa agtagcagat aataaaggaa atgaccgcac ggcagaattg
    51661 attgatcgtt tatacgatga agagaatgga ttaccgaaaa atatctttga tatcccgttt
    51721 ttcaaagaac aattaaaaca aatgaacatg atcccttgtg gctaccaccg ctattactat
    51781 cgtgaagaag aaatgctgga acatgcttta gaagaatatc aaacgattgg tacaagagcg
    51841 cagcaagtga aagaaacaga agcagagcta tttgaattat acaaagatcc agaattagac
    51901 cataaacctg aacaattgca aaaacgaggt ggcgcttatt attcagacgc tgcctgtgaa
    51961 acgattgctt caatctatgg aaataaagaa acacagatcg tggtatctac aaagaatgat
    52021 ggagctgttc ctgacctgcc ggcagattgt gtagtagaag tcactgccta tgttggcgca
    52081 caaggagcga gaaatgtagc gtttggttct cttcctgctg ctgaaagagg ctggttgcaa
    52141 gtaatgaaga atatggaatt gctgacgatc gaagcagctg tgactggtga ttatgggaca
    52201 gctttacagg cgtttacgat caatccactt atacgatcag gaaagacagc aaaacggatc
    52261 atggatgagt tgtttattgc acaccagcag tatttaccgc aattcaagga gacaatcagc
    52321 cgattaaaag aagaaggaat cgaagttcaa gatgaactgg caagagaatt atcaggtaat
    52381 acacatataa tggaggaagt ataatgggat tcagaaaaga ttttttatgg ggcggtgcta
    52441 cagcagccaa tcaatgtgag ggcggttatg atgaaggtgg acgcggacta gcaaacgtag
    52501 atgttgtgcc aatcggaaaa gaccgttttc ctgttatcac cggaaagaaa aaaatgtttg
    52561 aatttgatga ggatcatttt tatccagcaa aaattgcaat cgatatgtat catcgttata
    52621 aagaagatat tgctttgttt gcagaaatgg ggttcaagac ttatcgaatg tccatcgcct
    52681 ggagtcgtat ttttccaatg ggggatgaga aagagccgaa tgaagctggc ttgaaatttt
    52741 atgaggatgc ctttaaagaa tgccaaaagc acggaattga gccacttgtg acgattacgc
    52801 attttgattg tccaatgcat ttgatcaaag aatatggtgc gtggcgttcc agagaactcg
    52861 ttggttttta tgaaaatctg tgtcgagtca tcttcaatcg ctataaagga cttgtcaaat
    52921 attggctgac attcaatgag atcaatatga tccttcatgc accattcatg ggcgcagggc
    52981 tgtattttga aaaaggagaa aatgaagaac aaatcaaata tcaagcggca catcatgagt
    53041 tggttgcaag tgccatcgca acgaagattg ctcatgaagt agatccggag aacaaagtag
    53101 gctgtatgct ggcagcagga gcatattatc catacacttg caatcctgaa gatgtttggg
    53161 cttctagaaa atcagatcga gaaaattatt tcttcattga tgtccagtca cgaggagaat
    53221 atcccgctta tgcattgaaa gaaatggagc gaaatggcat tcagatcgaa atggaaccag
    53281 gggatgccgc attgctgaaa gaacatactg ttgattttat ctctttttct tattatgctt
    53341 cccgagtttc gactaccgac aacgaactct tggaacagac tgcaggaaat atctttgctt
    53401 ctgtcaaaaa tccatatctg caagcaagtg aatgggggtg gcaaattgat ccattaggct
    53461 tgagaataac gatgaatgat atttatgatc gttatcaaaa accattgttt attgtagaaa
    53521 acggcttagg tgcagtcgat acgccagatg aaaatggtta tgtggaagat gattaccgaa
    53581 tcgattacct ggcagcgcat atccaggcga tgaaggatgc agtagaacta gacggagtcg
    53641 atctattagg ctatacgact tggggatgta ttgatttagt atcagctggt accggtgaga
    53701 tgaagaaaag gtacggattc atttatgtcg atcgtgataa ccaaggaaat ggcacattag
    53761 cccgttcaaa gaaaaaatca ttcaactggt ataaaaaagt aattgattca aatggagaaa
    53821 atctttcgaa cgattgatca atgatttata aaagtctatt gtttttcagc ggatttttcg
    53881 agtataataa agctattcga gaaacgcaag cagaacaata gacttttttt cgcattggct
    53941 gtaaagtatg cttacttgat taagtaaagg agaatctaca tatgtatgat gtaatcgtta
    54001 ttggcgcagg tcctgctggg atgactgctg ctttatatgc ttcacgttca aacttatccg
    54061 tattgatgat cgaacaaggt gcccctggcg gccaaatgaa caatacggca gaaatcgaaa
    54121 attatcctgg attcgattcg atcatgggcc ccgatctagc ttataagatg tatgaaagtg
    54181 tctcaagatt tgggacagaa aatgcttatg gaatcgttca agatatcaag gatcacggca
    54241 gccataaaga agtcatctgt gaagataaaa gttacgaagg aaaaacagtc attatcgcaa
    54301 caggatgtat ccaccgtaaa ttaggcgtac ctggcgagga agaatttgct ggacgaggcg
    54361 tttcttactg tgcagtctgt gatggtgcgt ttttccgtaa caaacgtcta gtagtcatcg
    54421 ggggtgggga ctcagcagta gaagaagcca tctatttgac acaatttgct tctgaagtag
    54481 tgatcgtcca ccgtagagat gaattacgtg cacaaaagat catccaagac cgtgcatttg
    54541 caaatgaaaa aatttccttt atgtgggata cagtcgttga cgaaatcgtt ggaaacgaaa
    54601 tggtagtgac aggtgtgaaa gcacgtaatg tcaaaaccgg caatgaattt gatttagcag
    54661 cagacggagt attcatctat gttggactgg atccattaac tgcacctttt agagaagcag
    54721 gtttgacgaa cgaagctggc tggattccaa cagacaacga aatgaaaacg aatatgccag
    54781 gcgtttttgc catcggagat gttcgtgaaa aagatttgcg acaaatcact acagctgttg
    54841 gtgacggcgg tattgctgga caacaagtgt ataaatactt ggagtcttta gaagaaacaa
    54901 cagcataagt ttaaaaagag tagaagaacg ggttcaattg ttcttttact cttttttttc
    54961 gcgtgaaatt tactgaatct gatttatgcg aacacgtcca acaccttttg ataaaagaat
    55021 cctttcttga tttaagcgtt ttttggtcct catactttca tatctaataa gtgaatgcta
    55081 tacttattaa gaaccatatt tcagaaagag gtgttatttt catgtcttgg gaacaagtgt
    55141 atgaacaatg ggtacatgaa gaaaatttag aagaaaactt aaaaaaacaa ttaaatgatc
    55201 tgggtgaaga tcctgaaaaa cttgaagatg ctttttatgc accgttggaa ttcggtactg
    55261 caggtatgcg gggaatctta ggacctggga tcaatcgaat gaacatctat acggtaagac
    55321 aagcaacaga aggactagca cgtttcatgg atacacaagg tgaagaaaca aaacgccgag
    55381 gtgtcgctat cgcttatgac tcacgccatc agtcaccaga atttgcaatg gaagcagcca
    55441 aaacactagc acatcacaat atcccgtctt atgtattcga aagtctgcgt ccaacaccag
    55501 agctttcttt tgcagttcgt catttgaaga cgtttgcggg aatcatggta accgcatctc
    55561 acaacccagc ggcttacaat ggatataaag tctacggaga agatggcgga caaatgcctc
    55621 ctgcagacgc agatgcattg acgaaatatg tccgtgaagt cagcaatccg ctaaaagtcg
    55681 aagtattatc tgacgaagaa gctaaacata gcgggttgat cacgatcatc ggagaagaag
    55741 tggacgctgc ttatctagaa gaaatcaaag cagtgacgat tgatcgtgaa ttggttgaaa
    55801 caatggggaa agatttgaaa cttgtgtaca cgccacttca tggaacaggg aaaatgttgg
    55861 gcgaacgtgc gttgaaacaa gctggattcg aacaattcgt actagttccc gaacaagctg
    55921 tggcagatcc agatttcact acggtcaaat cgccaaatcc agaagaacat tcagcttttg
    55981 aatatgctat acgacttgga gaaaaagaag gagctgactt gctgattgca acagatcctg
    56041 atgcagaccg tttaggcgca gcggttcgtt tgccagatgg ttcttatgaa gtattgactg
    56101 ggaaccaaat cggagcattg tttgctcaat atattttgga agcacatcaa tcaagaggca
    56161 ctttaccaga aaacgcagcg gtattgaaat caatcgtttc cagcgaattg cctacagcaa
    56221 ttgcggaaca ctacggtgct accatgttca atgtgttgac agggtttaaa ttcattgctg
    56281 aaaaaatcca acaatttgaa gaagaccatt cacatacttt tatgtttgga ttcgaagaaa
    56341 gttatggtta tttagttaaa ccttttgttc gcgataaaga cgcgatccaa gcattgcttt
    56401 tattagcaga agtagcagct tactataaaa aacaaggcaa aacgttgtat gacggcttgc
    56461 aaacgatttt tgaaacctat ggctatttcg cagaaaaaac gatttctgtg acaatgagcg
    56521 gacaagaagg ttctgcgaaa atctctgctt tgatggaaaa attccgtaaa gaagcaccga
    56581 cagaatttgc cggagcaaaa gttattcaaa cagaagactt taaagaattg acgagaacag
    56641 accgtgaagg aaacgtagaa aaactaacaa caccgccatc cgacgtattg aagtatacat
    56701 tggaagaaaa tggctggatc gctatccgcc cttcaggaac tgagccgaaa atcaagtttt
    56761 atattggtgt aaaaggaagc tctacagaag acgcagccga aaaagtagcc tcttatgaag
    56821 cgattatcaa aaagatgaca gaagaataaa aaacttgaga ctgatggaaa aagtggtaaa
    56881 ctttagaagt cagtcttttt tcaagttgga ggtgaatgga ttgccagaca cgcaaaaatc
    56941 aaaagtagag aaagtcagtc aattattcaa aatgttgagt gatcccactc gtcttaaaat
    57001 tctgctatat ctgaaagacg gggaacaaaa tgtgacagca atcacccaag cagtggaaat
    57061 ggagcagtct gctgtatcgc atcaactacg tttattaaga gaaaatcatg tcgtcaagtc
    57121 tcatcgcgag ggaaaagcga ttttgtatag tcttgatgat tctcatgtac tggatatttt
    57181 gaaccagaca ctcaaacatg tggaacatca ataacgatag gtgaaaatag ataaacactg
    57241 gagtgtgaca acgaagaaat ttcgttgtca cactccagtg ttttttaaga aaaagacaaa
    57301 aatgattaat cgcttttttc tgttgtttca gttccataat aactatgatc ttctaggtct
    57361 aaagcattca aaataagcga agccgtttct tcaatagata aagatgccac attgatcact
    57421 acacatccta gtttttcata aagatcattc gcaaaatcga gttcagctct aattttttcg
    57481 atatcggaat aagcagtatc agggtttaat ccgtaagagc gcatccgttc tttacggata
    57541 ttatttaaaa tatctgggtt attcgttagt cccacgattt ttttaggatc gatttcccac
    57601 agctgtttgg gaatatgtgc ttggggaatc aaaggtaaat tagcaacttt taaattttta
    57661 tttgctaaaa acaagctcaa aggcgttttg gaagttcggg atacgcctag caaaacgaca
    57721 tctgcttcta aaaaaccacg aggatctttc ccatcatcgt attttacagc aaattccatt
    57781 gctttgatcc gtttgaaata gttctcattc aataaatgga gggcaccagg ttgccgaatt
    57841 ggcttgatac cagttaaacg ttcaatttct gatatcggtg gcgtcatcac atcgaaacta
    57901 aataattgat gttcttcaca aaaagaacgt gttatctcga tcagttcttg attgattaat
    57961 gtatgcagga caagtgcttt ttcttctaat gcatcttgca atgcctgcct taatgttttt
    58021 tcatctgtca cgaacgttct gcggaatagg ttgaactcaa cggtaggata ctgagccata
    58081 gtagcttgcg ctagttttgt cgcagtttct ccagcagagt cggagatgac aaaaaaagta
    58141 acgatttctg tagaatcgga agacatggat aaaacctcac ttgcattttt tttatgttta
    58201 ttataccata ctctagaatg cgtacttcgt aatcattttg atttggagga ataagtcatg
    58261 aaaaaacaga cattgatcga acaatcatta gaaacattga aaaataacgg ttttaaatat
    58321 actaaaaaaa gagaagcatt gttaacttat ttagtgaagc gtaaccgcta cgtatctgct
    58381 aaagaagttt ttgactatat gaatcaggaa ttcaaagggg taagttatga tacgatttac
    58441 cggaatctcc atgattttgc agagatcgat ttactggaag aaaccgattt aaatggtgaa
    58501 atgaagtttc gttttcattg ctgtcagggg gaaatcggcc atcaccatca ttttatctgt
    58561 acagtgtgcg gaacaacgaa agaaatccat atgtgtccaa tgaattattt cgaagaacag
    58621 ctaggtggat gcacgatcga agggcataga ttcgagattt tcggaagatg tgaaaattgt
    58681 caataaggat ttaaataaaa taacatttga taatttaaaa aaaaagcaag atttccttga
    58741 cggtcaaaac agcattagat ataatattta caggacagtc ttgttttttg agatatattt
    58801 caataacaat tacggttact ttttaagctc ggagggaggg aattaacatg tcaaaaacag
    58861 tcgttcgcaa aaacgaatca cttgacgatg ctcttcgtcg cttcaaacgt tccgtgtcaa
    58921 aagcgggtac tttacaagaa tctcgtaaac gtgaattcta cgaaaaacca agtgtaagac
    58981 gcaagaaaaa atctgaagca gctagaaaac gcaaaaaatt ctagttctgt gatgaattgg
    59041 agttggtagt gtgtcactac taagtagatt aaacgatgac atgaaagcag cgatgaaagc
    59101 gaaagataag gaatctttac aagtcatccg aatgatcaag tcatctatac aaaacgagca
    59161 gattaaagaa ggtcacgact tgacagaaga agaagaacta acggtgttgt ctcgcgaaat
    59221 gaaacagcga cgtgattctt tgcatgagtt tgaagaagca ggacgtgacg atttagctga
    59281 aaaagtaaaa agtgaaattg taatagtcga aaaatatatg ccggaacaac tttccgacga
    59341 agaaattcga caacttgttc aagaagcaat cactcaaact ggtgcttcat caatgaaaga
    59401 atttggcaaa gttatgggtg caattatgcc taaagtcaaa ggaaaagcag atggaaacca
    59461 agtgaatgcg attgtgaaag aactattaca atcttaattt tatcatttaa actgcggaca
    59521 gtgattgtcc gcagtttttt gtgttcgtaa aaaaagaact aaaaaacagc tagaagaaag
    59581 aataagattc tttcttctag ctgttttttt cctgtttaat atctcttatg ttaaattgtg
    59641 tttgatttgg ttattcttct ggtagttcat ttgcttcttg tttataccct gataggcgat
    59701 aggcgtaata aaagagtaag ctgccagcgt aaccaagtaa cgttgggacc atccagccaa
    59761 gtcctaatgc agcaaaagga acgtgatgat gataaaatcc tagtaagctt gctacgacag
    59821 tttgctgact cactaatgca ggcgaatttg ccaacatatc caatacggct ggaacggcag
    59881 aaaagaacat cgttacttga tagacaaatg gactcttttg gaagaatttt gctgttaagg
    59941 aaagcaagat taacgcaagt gacaatggat acaaaagcat caatactggt aaagaccatt
    60001 ggatgatatt atccaatccg gcattcgcaa cgacaaaaga gacaaaagtc gttaaccgca
    60061 gccatgtcat ataactgact ttagggaaca gtttatggaa atcttgtgca aaagaaacaa
    60121 cgagtcccat agcggtagtg aagacaccta atgtgaccat tacccctaag aagctagcac
    60181 cgaagttacc taaataaaaa ttagtgatct gagaaagtgc agttccgcca ttttctgata
    60241 aaggcatctt attcaaactc atcgcaccga gaaggaccag accaaaataa atcatgactt
    60301 ctaacgcaac gctcaggcta ccagcttttg ctgttaattc tgtgactttt ttatctttaa
    60361 aaccaagatc acgaactgca tggacaaaag tgacacttaa agcaagtaaa gctactgcat
    60421 caaccgtgtt gtatccttct aaaacgccat ttgtcaaagc gttcgtctga taagaatctg
    60481 cagcagattg gtttagattc cccatgggtt ggaataaaga aacgacaaaa atgatagcta
    60541 ataatgcaag aaacaaggag tttaaatact ttccgacata tttgaccaaa cgtgttggat
    60601 gtacagtaag gaaataagca agtaaaaaga aaagagcaga gaataaaaac atacctaatg
    60661 aatcatattt ttcaggcaaa aagggtttag cagccatttc aaaagcagtt gctgctgtcc
    60721 gcggtgtacc aaagaatggt ccgatcgtta aatgaacaag gatcaagaac aaggcagcat
    60781 accattttcc gacaggttta gctaagtcat acagaccatc actttttgtc actactacag
    60841 ctaagatagc taataatggg aaaagagcgc ctgagatggc aaatcctaca ccagcgctga
    60901 tccagtttga tccagccatt tgacctaagt ggacaggaaa gatcagatta cctgacccga
    60961 agaacatccc gaagataagt gaactgacca tcaataattg cttccatgaa taagtttttg
    61021 tttcttccat aaatttttcc tccaataatt atcattttga gtaacaaaat aaacgttgca
    61081 gaaactagaa atgccactaa tatcagattc catgaaaacc accaccttaa aatataaaaa
    61141 aaagcccttt tccacttggg aaaagggctc tagattctag aacggtacca ccttttttcg
    61201 cttaaaaaag cttcttcatt cacggacgct tttcgataaa gttatccgct agcctgataa
    61261 tggaggcagc cacaatgatc tacgttttcg acattgactc ggaaatgatg ttcgtctatt
    61321 cgatttattg tcatttctca cttacatgac tcactgggaa taaatcgctg aatagcctac
    61381 tgttttcctc gacgctttgc tgtatattta tttgttgacg ttataataca cctgtttttt
    61441 ttgaaatgca agaatatttc aaatattttg ttttcttttt ttgaaacaag aaaaataccc
    61501 agagaatact tgctaagtta gacggaatac ttgtcctcta tcttatgcat agcttacatg
    61561 atcagctttt tttattcaaa cgagaccttt agagaatgga agcagctatt ttttaacgtt
    61621 tcttggtact tatggttaag caactctttt agaacctttt cttatttgct atatagtttc
    61681 ttctgagtat ggtaaaatag gaaagaatga atcgaaaaaa aggggatgtc tatttttgac
    61741 ggaagagtct agttctttag agattagatt gacggctgct gatgatgtca gcatgctttt
    61801 aggtgcacac gacaagcata taaaactgat tgaagaaaca acagaaacaa ccatccatac
    61861 acgtggagaa atcattcaga tcattggcga acaatcccag atttccttgg ctgcctcagt
    61921 cattcggaca ttgcaggaat tgatcaaacg aggaatccat gtaagtacac cagatgtcgt
    61981 gacggcactg aaaatggggc aaaaaggcac attagattat tttgttgaaa tgtatgagga
    62041 agagatcgtc aaagaccgta atggaaaacc aatacgggta aaaaacagcg gccaaaagaa
    62101 atatgtcgaa tcggtagcca aacatgatat cgtatttggc gtaggaccag ccgggacagg
    62161 aaaaactttt cttgcagtag tattagcaat cgctgccttg aaaaaaggtg aagtgcaaaa
    62221 aatcattttg acgcggcctg ctgtggaagc aggggaaaat ctaggctttc ttcccgggga
    62281 tctaaaagaa aaagtagatc cttatctgcg tcctgtttat gatgctttat accaaatttt
    62341 tggattagat catacgaacc gattgatgga acgaggcgtg atcgaaattg ctcctttagc
    62401 ttacatgcgg gggcggacat tggatgatgc attcgttatt ttggatgaag cacagaacac
    62461 gacagttgct cagatgaaaa tgttcctgac acgtcttggt tatcaatcaa aaatgattgt
    62521 caatggagat acgagtcaga ttgatctgcc tcgagggaca acaagtggtc ttgttcatgc
    62581 agaacgaaca ttaaagcaaa tcaagaaaat cgattttgtt aattttgaag caagtgacgt
    62641 tgttcggcac cctgtagttg ccgaaatcat caaagcttat gaaaaggcag atctccatca
    62701 agaataagct ttgcttagga ggataagcac atgttgaata aaacactgca gaaactacaa
    62761 gaaaaacttg gaaaggcagc attgcctttc ctgctgttct tgttttttat cattatctgc
    62821 ggcgttacat ttagtagtgt caaacaaaag gccaatgatt tcagagaagg ccaagtagca
    62881 gaagaaagta tccgtgcgaa caaaacgatc gagaacacgg aagaaaccga acaaaaaaga
    62941 aaactggcag cagaagcagt cactccagaa tacacttacc aaaaagatct tgcagatgac
    63001 cagaacaatc ggatcaagca actatttgaa ttgatcgata aaacgaatga tgccatcaac
    63061 aaatcttatg acgaaaagat tgataaggca aaggaagatg aaaatatccc aaagcctact
    63121 acagaagagc ggatcgccgc gttgaagaaa aattttgaaa atgtgaatgc agaaaatgtt
    63181 gcgttttatc aaaaactacc gaacaatttt tattcgacga tatttgaaat gactgaaact
    63241 gagatccata ctgtgaggga tgagagtctg aagctcattg acgaacagat gagcaaacaa
    63301 gttcgtgaaa gtgaattgga agcttttaaa caagaagcag aagagcaaat ccaatatttg
    63361 aatgtcacac cagcacaaca acaaatgatt cgctacttag tggatcaagg tatcgtagtg
    63421 aatgacgtat tgaatgagaa aaagacagaa gaattgaaac aatccgcacg agaagctgta
    63481 caaccagtga tgatcttcca aggagaaatc attgttcgtg aaggtaatca aatcgatgct
    63541 tcagctatga aaaaattgga attactagga ctgactaatc aaacaacttc gatttttccg
    63601 ctaattgcca tggttttagc tgttttgctt caaatcggcg tactgattta taattcactt
    63661 caattccaaa atgcaggaga aagaacaaaa tacgtgcttt tctatgtgac agctatgtca
    63721 gtcagcatag tgctgatgaa atttttccaa ttattccaaa cggaacaagc tacttatatc
    63781 ccgctgtttt atcccgctgc ttttgcacca cttgttttaa gctttttctt gaaccgaaga
    63841 gcagggatca tggctggtat tttccaagca gtatctgcac ttttcatttt ttatgattcg
    63901 attggcacga actttctaac gattatcttg atgtcttatg ttttctctgg gctcatggca
    63961 acgattgtca gacgcaaacg gatctcagaa caaggattat ctgctgccat gtgggtcatt
    64021 atttttcctg tgatgatgga tgtcattttg atcgtctatc aaggaatgag ctttggtgat
    64081 gcaacgactt ggttaatgct tgtctgcggg ttcactggtg cactcttctc attcttgctg
    64141 acgatcggtc tacacccata tattgaattg atggtcaatg atgacagtgt aattgtatta
    64201 aatgaactca gcaatccaaa tcatccttta ttgaaacagt tgctggaaga agcgccaggt
    64261 acttatcatc atagtatgat ggtagctaat ctaagtgcta acgctgtagc agaaatcggc
    64321 ggacgttctt tgctaactcg agtagcttgc tattatcatg atatcggcaa gatcaaacat
    64381 gctagctttt tcgttgagaa cttgccttca ggagcggaaa acccgcacaa tttcttattg
    64441 ccagaagaca gtaaacatat tatctttgga catgtgacag atggagccaa aatattggaa
    64501 gattataata tgccgcaaat ggtgatcgat atttgcaaac agcatcatgg aaccacgcta
    64561 atgcgttatt tctacatcaa agcgaaagaa cgcaatccgg aagtaacaga agagcaattc
    64621 cgttatcctg gaccaaaacc gcaaagtcgt gaagcaggga tcgtcaatat tgctgacagt
    64681 tgtgaggctg cagtacgggc aatggatcat ccgacgagcg aaaaaatcga aagttttgtc
    64741 cataatctta tcgaagagcg tatttccgat ggtcaattag acgatagtgg actgaccttg
    64801 aaagaaatcc gaaaagtgga aaaatcattg atcagcggtt taagtagcac cttccattct
    64861 cggatcaaat atcctaaaat gaaaacagaa gttgagaaga taaaggaaga acaagaagaa
    64921 tagaaagaag cgatgtataa tggatattac ctttatggat gaaacaaacc aagtctcaga
    64981 agagaaaatg aaagaaatcg atgacttgct gcaatttgca gcatcttatt taaagctgcc
    65041 agaggaaaca gaaatgtctg ttactttcat ggacaatgcg gcaatccaag taatcaatcg
    65101 tgattaccgt ggaaaagatg caccgacaga cgtcatcagt tttgctctgg aagaagaagg
    65161 cgaagacgaa atcccagtga tttttgaaga tggagagaat ccattgcctc gtgtactagg
    65221 agacatcatg atttctattg aacgtgctgc tgaacaagca gaagcttacg gccattcata
    65281 tgatcgggaa cttggctttt tagcggtaca tggattcttg catatcaacg ggtatgacca
    65341 tatgacacca gaagatgaga aagaaatgtt tggtttacag aaagagatct tggatgccta
    65401 tggactcaaa agataaaaac gtagagaaaa acaaacattt cctgatgtct gtggaatttg
    65461 ctgtccaagg agtcaaaacc gtttttgagg aagaacgcaa tatgaggaaa catgttgcgt
    65521 tcggtatcct tgctttattt atgggcgtga ttttccagct tgatcggata gagtggttat
    65581 ggctgctttt agctgttttt ctcgtttgga tagtagagat catcaatact gtttttgaaa
    65641 atgtagtgga catgtttacg gattttcatt ttcatccgat tgggaaaaaa atcaaagata
    65701 tggcagctgg agcagtttta gtgaccgctt gttttgctgt catcattggg ttgattttat
    65761 ttgtccctaa aatatatcat ttgttgttta attaaaaagg agaacgtata tgccagaaca
    65821 taaatcaggg tttgtcgcaa tcgtcggccg cccgaatgta ggaaaatcca cattattgaa
    65881 ccgaatcgtg ggtcaaaaaa ttgccattat gagtgacaag gcacagacaa ctagaaataa
    65941 aatccaagga gtatatacta caccagaagc tcagctgatt tttatcgata caccaggaat
    66001 ccataaacca aagcatcgtc taggagattt tatggtagaa gcagcttaca gtgcgttgaa
    66061 agaagtggat gcagtcttat ttatgatcag tgctgatcaa aaacgcggcc ggggagacga
    66121 ttttattatt gaacgtctaa aaaacgtgca ttctccagtt tatctgatta tcaataaaat
    66181 cgataagatc catccagacg agttgctggg aatcatcgaa gattattcga cgcagatgcc
    66241 ttttgcacaa gtgattccga tctctgctac agaaggaaac aatgtcgaac gcttaatgga
    66301 tgtattagtc agtgaaatgc cagagggccc acaatatttt ccagatgatc aagtgactga
    66361 tcatcctgaa tactttatcg tttctgaatt gatccgtgag aaagtcttgt ttttaacacg
    66421 agacgaagtg cctcattctg tcgcagtcgt tgttgattcg atgaagcgaa acgagaatga
    66481 taaaatccat atccaggcca cgatcatcgt cgaaagagat agccaaaaag gaatcattat
    66541 cggcaaaggc ggaaaaatgc tgaagcaagt aggaacgaaa gcaagaaagg atatcgaaaa
    66601 tcttttagga gacaaagtat ttctagaact ttgggtcaaa gtccaaaaag actggcgaga
    66661 caaaaaagtc tatctgcaag actttggcta ccgaaaagac gaatactgat attctgaact
    66721 tcttataggt taagtataga aataaactgg ataaaaatcc gcacgttgag aaaaacggcg
    66781 gaagatcgaa acataaataa tctggatcgg cagaatctgc ttttccagat ttatttttta
    66841 ggagggagaa tatgagccag cttgcagaat cgaaaggctt gattttatct agccgtaatt
    66901 acaaagaaaa agataaatta gtcaaaatct ttacggaatc ttctggcaaa atgatgtttt
    66961 atatcaaagg aatccatcgg caaaatcagc cattagctcc ggctttgctc ccctttaccg
    67021 aagcagtcta tatcggtaat tttcgaagtg aaggtttgtc gtttttaagc agtgtcaaag
    67081 acgtccatgc gtttcggaac atccaagaag atatcttttt gagcggttat ggtacctatt
    67141 tgctaaatct agtagatgca gcaatcgaag atcatcaata tgacccgcat ttattccaat
    67201 ttacgcaaca agctctgcag cggatggatc aaggagatga tgcagaaatc atcacgaata
    67261 tctttgaagt tcaaatactg caacgcttcg gaatcgcgcc aatttggacg cactgtgtcg
    67321 tatgcgggga aacaaaagga aaatttgact attcttccaa atctggcggt gtgatatgcg
    67381 agaaacactg gccaatggac gaacaccgtt atcatgcaga tcctcgcgct gtttatttcg
    67441 ttcgaatgtt ttcggctatt tcttatgata agatcagtgg aatccacttg aaagaagaaa
    67501 cgaagcaggc aatccgtgac ctcattgacc agttgtatga tgaatatgtc ggtattcatc
    67561 taaaaagcaa aaagttcatt gatcaaatga agtcttgggc acacgtatta aagacgccag
    67621 agaaaaatat gcctgaaaaa gagatacctg aaaaagaaga ataaatgaaa gatacaacaa
    67681 aaaaatcaaa actatttgac aaatacaggg acttccgtct attattaacc gtagattaaa
    67741 tagatcgttc actgagagga tctattatat aaacaagaaa actgcaaaat gaatacgtac
    67801 attgaccaag atcagtaaaa aacacgtggt ttagcgagtt cgggagagtg gaagccggac
    67861 ccaaacgatt tttgaagagg cacttgcgag tacgataaat gaagctgccg aatctttatt
    67921 cggaagtagg gtggaaccgc gaaattattc gtccctatgt ctgtaatcag acatagggac
    67981 tttttgtcgt tttctttcct gtattcttat tgacagcaac gcttaaaaat tgaaaggggc
    68041 aaaacagatg agtaaaaaat tgaccgtcca agatatgatt ttaacattac aaaaattttg
    68101 gtcatcaaac ggctgcatgc tgatgcaagc ttatgacaca gaaaaaggag caggtacgat
    68161 gagcccatat acattcttac gggcaatcgg accggaacca tggaatgctg cttatgtaga
    68221 gccatcccgc cgaccagctg acgggcgtta tggagaaaac ccaaaccgtt tgtatcagca
    68281 tcatcaattt caagtcgtaa tgaaaccttc acctgaaaat atccaagagc tttatttaga
    68341 aagcttgcgt cttttaggaa ttgatccatt agaacacgat attcgttttg ttgaagacaa
    68401 ctgggagaac ccatcgatgg ggtgtgccgg tgtgggttgg gaagtatggc ttgatgggat
    68461 ggagatcaca caattcacgt acttccagca agtcggagga ctaccttgta aaccagttac
    68521 ttctgagatc acttatggac tagaacgtct tgcttcatat attcaagaag ttgaaagtgt
    68581 ctatgatctg gaatgggctg atggtgtgaa atatggcgag atcttcaaac aaccagaata
    68641 tgaacattcc aaatattctt ttgaagtaag tgaccaagac ttgctactaa gcaactttga
    68701 ccgttttgaa aaagaagcta aacgctgtat cgatgaaaat cttgttcatc cagcgtatga
    68761 ttatattttg aaatgcagcc atacattcaa tttattagat gctcgcggtg cagtatccgt
    68821 aacagaacga gcaggttatc tttcccgtat ccgcaacatg gctcgtgctg tagccaaaat
    68881 ctttgtggca gaacgcgaaa aattaggatt ccctttattg aataaaaaca cagcaaatcc
    68941 agaaaacatg aaggaggctg aataagatgg caaaagactt attacttgaa atcgggctgg
    69001 aagaaatgcc tgctcatgtt gtcacaccaa gcatgaaaca attagaagaa aaaacagcaa
    69061 aatttttaga tgaaaaccaa ttgacttatg atacgatcga aacgttttct acaccgcgcc
    69121 gcttagcaat caaagtaaca aacattccag aacgtcaagc agacagtgaa gaagaagtca
    69181 aaggaccagc caaaaaaatt gctttggacg cagatggaaa ctggacgaaa gcagctgaag
    69241 gatttgtccg cggacaaggt ctgacgacag atgctatcac ttttagagaa ttaaaaggcg
    69301 tggaatatgt ctatgtaacg aaatacactc acggcaaaga agcgaaagaa gtattgacag
    69361 gtttagcaga tgtcattaaa agcctcactt tcccagtaac gatgcattgg gcagaatacg
    69421 attttgaata tatccgccct attcactgga tcgtggcatt gttagatgac gaagtaatcc
    69481 cgtttgagat cttagatgtc aaaacaggca gagcctctcg aggacatcgt ttcttaggcg
    69541 aagatgtgac atttgcgcat gcaaaagaat atgaagaaaa attgaatgaa caattcgtta
    69601 ttgcttcacc gcaaaaaaga caagaaatga tcgttacaca agcaaatgaa ttagccgaaa
    69661 aaaatcactg ggttattgat ctagacgaag atttactaga agaagtcaac aatttagtcg
    69721 aatatccaac tgtcttttct ggcgacttcg aagaaaaata tctttctgtt cctgaagaag
    69781 tactagtcac ttctatgaaa gaacatcaac gttatttcga ggtacggaac gatcaaggta
    69841 tgctgcttcc tcattttatt tctgctcgta atggtgatcg ggtaaaattg gaaaatgtgg
    69901 ttcgaggcaa tcaaaaagta ttgacggctc gtttagaaga tgcggaattc ttttataatg
    69961 aagacaagaa agcttcaatc gaccaatacg ttgaaaaact aaaaactgtt actttccatg
    70021 aaaaaatcgg tagtatctat gagaaaatgc agcgggtaca agtcattgcc cgtatcattg
    70081 gcgaacaagt aggactatct gaggaagaat tgatggatct gcaacgagct gctgaaatct
    70141 ataaatttga tctagtaacc aacatggtag gggaatttcc tgaactacaa gggatcatgg
    70201 gcgaaaaata tgctctgctg aaaggcgaga aacctgctgt agcaacagcg attcgtgagc
    70261 attacatgcc tatatccagt gaaggagagc tacctcagtc tacagtaggt tctgttttag
    70321 cgattgcaga taaattagat agcatttttt ctttctttgc agttgggatg attccaagcg
    70381 ggtcaaacga tccttatgcc ttacgtcgcc aagcatacgg tgtcgttcgg atcatcgaat
    70441 cgaaaaaatg ggcctttcct ttgtctgttc tgcaagaaac gattagtgat gttatcagta
    70501 aagatacgga tcgtttcggc attggtttat cagaagggca acaacaggtg atcgacttta
    70561 tcaaaggccg tctgcgccaa ttattgacaa cgaagaatat tcgtcatgat gtcatcgaag
    70621 ctgttttgaa tgcagaacaa aaagatctga caaaagtatt tgcagcagca caattgttca
    70681 agcagcatct tgcagatgaa gacttcaaac catcgatgga agctttgaca cgtgtcgtga
    70741 acttagcgaa aaaagcggag cttgaaaaac aatcagaagt agaccctgaa ctgttcgaaa
    70801 atgaagcgga gaaagaactg cacaaagcag tagaggcaat gaaaaaaagc tttgctgacc
    70861 aaacgatcaa cgaaaattat gatcagcttg taagccttcg cccactgatc gaaaattatt
    70921 ttgatcagac gatggtcatg gcagaagaac cagcagtacg taagaaccga ttaacgcaat
    70981 taagtcaaat tgctgccatg gctttgtcgc tagcaagttt agatcaaatc atcacaaaat
    71041 aatttctaat atgtaaataa accagagaat aggcacaaaa
//
LOCUS       DAJSBL010000007        69518 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.007, whole genome shotgun sequence.
ACCESSION   DAJSBL010000007 DAJSBL010000000
VERSION     DAJSBL010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 69518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 69518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..69518
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.007"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..2387)
                     /gene="esp"
                     /locus_tag="ONT85_000576"
     CDS             complement(<1..2387)
                     /gene="esp"
                     /locus_tag="ONT85_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HCR2377573.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLIGVGLVLGTT
                     ELVKAQDEISPSSPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEKPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSSSAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGTKVADLAIERREGHENYGIGSITNNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSISDVYNKYFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDIYENPGENIPAGYHKVTFTAGEGTSIESGTTVFAVKDGVS"
     gene            complement(3004..3438)
                     /locus_tag="ONT85_000577"
     CDS             complement(3004..3438)
                     /locus_tag="ONT85_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2377574.1"
                     /translation="MRLYHLRSLEELRKLDFNKKFFWEISDLSCFVESERLSNGNNSV
                     LNEYDKVVINKVKSLCEEARESISHINGSNFKQTLFNLIKLDAKISSYLFFLIHDEFM
                     DYQKLDQLIERESWEDYYVGLTFSEKLNNYKLIDYKYLSESN"
     gene            complement(3455..4660)
                     /locus_tag="ONT85_000578"
     CDS             complement(3455..4660)
                     /locus_tag="ONT85_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HCR2377575.1"
                     /translation="MFSDKEVLLKSLHVCLKLPIVVFNSEFSIIKEYRSDRTTYFFYD
                     YKKLLTDNIKKTKDFHFFTGDFNEMFLLYMFKTRYYLFGPFRANNIDKVFFKLKMNNL
                     NVAMADRERLYNSLQNLTLYSLGDIRDILILVHYFFTGKIEDLFHEPLIEYTENLSKT
                     IEQIKIDNLLSQNYDPEIYLFLYENKILEYVKNGDIRNLENMVFNLSNGIIPSVSGDT
                     IRSEKNYSIIVFEKLAQTSITLGMDIIEAYQSRDALIQENELAVSLPEVLKVRDSGIV
                     YYTKEIGKTKIEHLSPLISSVVQFIGLNIYKRITVKEIANYFSVSETKLRESFKNEMH
                     ITIYNYISKRKISTAKIMLKSNHTISEVSLGLGFSDSSHFSRVFKKYAGVSPKQYQLG
                     LVDNFNNIS"
     gene            5354..5740
                     /locus_tag="ONT85_000579"
     CDS             5354..5740
                     /locus_tag="ONT85_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HCR2377576.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFDKTDKEIG
                     KVLGITRQGATNLKHRLYKKIQKMLV"
     gene            5834..6025
                     /locus_tag="ONT85_000580"
     CDS             5834..6025
                     /locus_tag="ONT85_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR2377577.1"
                     /translation="MNSLKEDFILAKAGNEEAVEAILKRFSSLIHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
     gene            6539..6871
                     /locus_tag="ONT85_000581"
     CDS             6539..6871
                     /locus_tag="ONT85_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR2377578.1"
                     /translation="MRKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            7167..7322
                     /locus_tag="ONT85_000582"
     CDS             7167..7322
                     /locus_tag="ONT85_000582"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HCR2377579.1"
                     /translation="MPTVIHSDRGSQHRSFIYQELLNDHHITHSVSQTGTPVDNAVIE
                     AFHRSIK"
     gene            7598..8938
                     /locus_tag="ONT85_000583"
                     /pseudo
     CDS             7598..8938
                     /locus_tag="ONT85_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(9179..10795)
                     /locus_tag="ONT85_000584"
     CDS             complement(9179..10795)
                     /locus_tag="ONT85_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HCR2377580.1"
                     /translation="MKKIPITILTGYLGSGKTILLNHILYGDHGKEIAVIVNDIGEVN
                     VDGVLVDKKGFKRTGEKLVSMPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGIS
                     KPIPIIQAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVV
                     EEEQDIRGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVD
                     LSKILNTNLFNIDETSYSVGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFA
                     HLTDKNFPGNITRAKGFLWFAQHSDFSTIFETSGSYKSIESLNYWVASLPKIECQMII
                     QKNPELKEAWHPLFGERINKLVFIGMKMDQQCIKEQLEGCLTDSLNADNFRKISPFPW
                     KKSNMFSQISLMKFLRKAGVKIGDVIIIGILIITSFIPLFVFSIEKENETTEDAIKYA
                     IVRIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVRTGW
                     ISKPGQTSICLPHKLVISIEKKESTDYYIY"
     gene            10913..11131
                     /locus_tag="ONT85_000585"
     CDS             10913..11131
                     /locus_tag="ONT85_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR2377581.1"
                     /translation="MSQYRHLTILERERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTIHLPKRKKIMLIEEKNADVITF"
     gene            11326..11787
                     /locus_tag="ONT85_000586"
     CDS             11326..11787
                     /locus_tag="ONT85_000586"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HCR2377582.1"
                     /translation="MHHRGKTRHTKNHVESRGKICLTHSIHGRPKSADDRSDFGHWKA
                     DIVAGKTGGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEALNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(11980..12219)
                     /locus_tag="ONT85_000587"
     CDS             complement(11980..12219)
                     /locus_tag="ONT85_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR2377583.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYRFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(12243..13766)
                     /locus_tag="ONT85_000588"
     CDS             complement(12243..13766)
                     /locus_tag="ONT85_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HCR2377584.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLKQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKINKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNELHNWPTFYPASLSKHEIAQEMMAH"
     gene            complement(13784..13906)
                     /locus_tag="ONT85_000589"
     CDS             complement(13784..13906)
                     /locus_tag="ONT85_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HCR2377585.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKRNKMKKLA"
     gene            complement(13936..14919)
                     /locus_tag="ONT85_000590"
     CDS             complement(13936..14919)
                     /locus_tag="ONT85_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HCR2377586.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVMADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLKKFNHPLVFESDSSENH
                     YNGKYYHQHHQVHEFKSILLTSDQDIKKDFLLQWIDWLILTNQGSLYRFKGLIALQEQ
                     DFIVAMQEVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            complement(14943..15092)
                     /gene="rpmG"
                     /locus_tag="ONT85_000591"
     CDS             complement(14943..15092)
                     /gene="rpmG"
                     /locus_tag="ONT85_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HCR2377587.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            complement(15113..15490)
                     /locus_tag="ONT85_000592"
     CDS             complement(15113..15490)
                     /locus_tag="ONT85_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2377588.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            complement(15522..15701)
                     /gene="rpmF"
                     /locus_tag="ONT85_000593"
     CDS             complement(15522..15701)
                     /gene="rpmF"
                     /locus_tag="ONT85_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HCR2377589.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            complement(15716..15985)
                     /gene="rpsN"
                     /locus_tag="ONT85_000594"
     CDS             complement(15716..15985)
                     /gene="rpsN"
                     /locus_tag="ONT85_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HCR2377590.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            complement(16508..18274)
                     /locus_tag="ONT85_000595"
     CDS             complement(16508..18274)
                     /locus_tag="ONT85_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR2377591.1"
                     /translation="MQMIGGLTMKSFYKNKFALSDQGAEALTKASISSFFVYCINMVP
                     AFIIMMLIDELVLENTKPRWLYFAVSFVTLLFMYWLLDREYENLYNSTYKESAHLRVQ
                     IADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVMLL
                     VGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEINS
                     FNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLLTG
                     EVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSPLK
                     SFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDYDE
                     GCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAARL
                     ANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLDAE
                     NEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKLVDQGTHNELYRRSEIY
                     KNLIKKTKLSEKFVYEKEAQSR"
     gene            complement(18234..19988)
                     /locus_tag="ONT85_000596"
     CDS             complement(18234..19988)
                     /locus_tag="ONT85_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR2377592.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLQ
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQHLMKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHQVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            complement(20098..20667)
                     /locus_tag="ONT85_000597"
     CDS             complement(20098..20667)
                     /locus_tag="ONT85_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HCR2377593.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVASWALMQLGSAAGQWIPIWFFTDRFRQDWIDKGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
     gene            complement(20685..22100)
                     /locus_tag="ONT85_000598"
     CDS             complement(20685..22100)
                     /locus_tag="ONT85_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HCR2377594.1"
                     /translation="MFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSGKST
                     LLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQDPRGQFFTTNTTS
                     ELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLSLNS
                     KLIILDEPSSNLDFKATKKLADLLCDLKNKGFTIVIAEHRFYYLKELIDQVLFVKNRT
                     VKTTTIQLLKTENNNALRKFDIFNSSIEVPYIEKVSADVLKVEEITYENILKDINFDV
                     KVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIENKKNLSSPFLVMQEMDYQFFTE
                     SVRSELALGNEIVSKDQQEKILKKMELYKMKDQLPFDLSGGEKQRLLVSIASLSKTNL
                     FLFDEPTSGLDYINMERIADLIKDLKEKGAVVIATHDPEFLYKTCNRIIYLKNGGIKK
                     DIKLRVEDSTLIEQIFNDITK"
     gene            complement(22111..22779)
                     /locus_tag="ONT85_000599"
     CDS             complement(22111..22779)
                     /locus_tag="ONT85_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HCR2377595.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFATSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            complement(22909..23493)
                     /locus_tag="ONT85_000600"
     CDS             complement(22909..23493)
                     /locus_tag="ONT85_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCR2377596.1"
                     /translation="MESGLSHFEKEGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLI
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
     gene            complement(23824..24057)
                     /locus_tag="ONT85_000601"
     CDS             complement(23824..24057)
                     /locus_tag="ONT85_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dependent repressor"
                     /protein_id="HCR2377597.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLAKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            complement(24072..25022)
                     /locus_tag="ONT85_000602"
     CDS             complement(24072..25022)
                     /locus_tag="ONT85_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HCR2377598.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSINSKDKDKVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            complement(25019..25861)
                     /locus_tag="ONT85_000603"
     CDS             complement(25019..25861)
                     /locus_tag="ONT85_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HCR2377599.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVIVVLFFKELLIT
                     SFDPLMAKAIGMNVSFYHYLLMVLLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGASSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERSL"
     gene            complement(25863..26582)
                     /locus_tag="ONT85_000604"
     CDS             complement(25863..26582)
                     /locus_tag="ONT85_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HCR2377600.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKKLKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFIGEGE"
     gene            27274..27432
                     /locus_tag="ONT85_000605"
     CDS             27274..27432
                     /locus_tag="ONT85_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR2377601.1"
                     /translation="MNKKQTCAFLGIDNNTLDKWIRIYNFPVIRVGGVVRFDVEDIKD
                     FMNTFKIT"
     gene            27504..28730
                     /locus_tag="ONT85_000606"
     CDS             27504..28730
                     /locus_tag="ONT85_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR2377602.1"
                     /translation="MPTKLSNGKYKTNLRYPKRFKEITGIASEKYQKTFPNRQLAIKA
                     ENDMKKKIEKVLREENANSLELKGKITFKKFYESKWLPRYELGQTIRSNRPPSDITIS
                     NTKDIFRLHILPMFGEYAMNYLNINTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILNYIEFNRTTKIIQSITAPKKNALEEKRIQEGKQALSSKELTNWIEAVNDDFNNHLL
                     TFHDYTLFMITLYLGDRKSETYALQWKYIDFEKQTVRLKHTLDKYQRKKFTKGRKDTV
                     IQVPEVVMTLLSEWKSVQADQLLKLKIKQTLDQYLFTYTKPSGEVNCPVHADYLNYRI
                     NSIKRRHPDLVHLSPHKLRHTYATIARQGGADMNQISNALTHSDISTTKIYVNTPDIV
                     DKAVFEAFQRGLNKCD"
     gene            complement(28818..29210)
                     /gene="rpsI"
                     /locus_tag="ONT85_000607"
     CDS             complement(28818..29210)
                     /gene="rpsI"
                     /locus_tag="ONT85_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HCR2377603.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(29226..29669)
                     /gene="rplM"
                     /locus_tag="ONT85_000608"
     CDS             complement(29226..29669)
                     /gene="rplM"
                     /locus_tag="ONT85_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HCR2377604.1"
                     /translation="MRTTYMAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPT
                     FTPHLDTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLI
                     ETSVKGMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            complement(30188..30655)
                     /locus_tag="ONT85_000609"
     CDS             complement(30188..30655)
                     /locus_tag="ONT85_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HCR2377605.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(30775..32313)
                     /locus_tag="ONT85_000610"
     CDS             complement(30775..32313)
                     /locus_tag="ONT85_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HCR2377606.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            32393..33079
                     /locus_tag="ONT85_000611"
     CDS             32393..33079
                     /locus_tag="ONT85_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HCR2377607.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(33280..33807)
                     /gene="msrA"
                     /locus_tag="ONT85_000612"
     CDS             complement(33280..33807)
                     /gene="msrA"
                     /locus_tag="ONT85_000612"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HCR2377608.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(33891..34568)
                     /gene="pgmB"
                     /locus_tag="ONT85_000613"
     CDS             complement(33891..34568)
                     /gene="pgmB"
                     /locus_tag="ONT85_000613"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HCR2377609.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(34565..35695)
                     /locus_tag="ONT85_000614"
     CDS             complement(34565..35695)
                     /locus_tag="ONT85_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HCR2377610.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            complement(35695..37878)
                     /locus_tag="ONT85_000615"
     CDS             complement(35695..37878)
                     /locus_tag="ONT85_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HCR2377611.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(37875..38900)
                     /locus_tag="ONT85_000616"
     CDS             complement(37875..38900)
                     /locus_tag="ONT85_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR2377612.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(38945..39772)
                     /locus_tag="ONT85_000617"
     CDS             complement(38945..39772)
                     /locus_tag="ONT85_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HCR2377613.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(39769..40836)
                     /locus_tag="ONT85_000618"
     CDS             complement(39769..40836)
                     /locus_tag="ONT85_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HCR2377614.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKHGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLIGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(40862..41698)
                     /locus_tag="ONT85_000619"
     CDS             complement(40862..41698)
                     /locus_tag="ONT85_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCR2377615.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(41695..42564)
                     /locus_tag="ONT85_000620"
     CDS             complement(41695..42564)
                     /locus_tag="ONT85_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCR2377616.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(42634..43917)
                     /locus_tag="ONT85_000621"
     CDS             complement(42634..43917)
                     /locus_tag="ONT85_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HCR2377617.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(43931..45616)
                     /locus_tag="ONT85_000622"
     CDS             complement(43931..45616)
                     /locus_tag="ONT85_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HCR2377618.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGARVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            45891..46916
                     /locus_tag="ONT85_000623"
     CDS             45891..46916
                     /locus_tag="ONT85_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR2377619.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            complement(47511..51185)
                     /gene="rpoC"
                     /locus_tag="ONT85_000624"
     CDS             complement(47511..51185)
                     /gene="rpoC"
                     /locus_tag="ONT85_000624"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HCR2377620.1"
                     /translation="MKWREPLLIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYR
                     TLKPEREGLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHI
                     ELAAPVSHIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTER
                     EYREKREQYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLD
                     ILEAFRASGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLK
                     RLLDLNAPGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRF
                     RQNLLGKRVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKN
                     AKRKIERQEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVC
                     EAYNADFDGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYY
                     LTMEEEGREGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMI
                     TTVGKIIFNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKK
                     KNLGNIIAEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIEN
                     AHKQVETITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSG
                     ARGNISNFTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTA
                     LKTADSGYLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTR
                     KEVRHPETNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNL
                     ATGSDVEVGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNP
                     KGQAVITEVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGA
                     PLTEGSIDPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRV
                     MDPGDTDILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAAS
                     FQETTRVLTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVY
                     SISDIEAQMAAEDAMNNQTK"
     gene            complement(51243..54860)
                     /gene="rpoB"
                     /locus_tag="ONT85_000625"
     CDS             complement(51243..54860)
                     /gene="rpoB"
                     /locus_tag="ONT85_000625"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HCR2377621.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            55588..56556
                     /locus_tag="ONT85_000626"
     CDS             55588..56556
                     /locus_tag="ONT85_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR2377622.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            56574..57479
                     /locus_tag="ONT85_000627"
     CDS             56574..57479
                     /locus_tag="ONT85_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR2377623.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            57476..58282
                     /locus_tag="ONT85_000628"
     CDS             57476..58282
                     /locus_tag="ONT85_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR2377624.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            58418..59407
                     /locus_tag="ONT85_000629"
     CDS             58418..59407
                     /locus_tag="ONT85_000629"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HCR2377625.1"
                     /translation="MGKELDAMTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKA
                     IKELEKAGYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDAK
                     LAAAQGDSVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTII
                     AASACVKAIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLN
                     FSVPQNHFPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKS
                     FVLGKTVSFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(59724..60248)
                     /locus_tag="ONT85_000630"
     CDS             complement(59724..60248)
                     /locus_tag="ONT85_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HCR2377626.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            60535..62346
                     /gene="scm"
                     /locus_tag="ONT85_000631"
     CDS             60535..62346
                     /gene="scm"
                     /locus_tag="ONT85_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HCR2377627.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSSTSSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDT
                     SASSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDTS
                     ASSDSTDTSTTTSDSTDTSASSDSTDTTSTTSDSSDSSTTTSDSTDTSASSDSTDTSS
                     TTSDSSDSSTSSDSTDTSSTTSDTRHASVSFSKTSSDNRTNFNLTNKTSNDNGNKSKR
                     ALPKTGSQSNNWITLAGVILLVIGLRITFMSYKKSKR"
     gene            complement(62501..63487)
                     /locus_tag="ONT85_000632"
     CDS             complement(62501..63487)
                     /locus_tag="ONT85_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HCR2377628.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(63510..64076)
                     /locus_tag="ONT85_000633"
     CDS             complement(63510..64076)
                     /locus_tag="ONT85_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HCR2377629.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKQDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            complement(64090..64506)
                     /locus_tag="ONT85_000634"
     CDS             complement(64090..64506)
                     /locus_tag="ONT85_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HCR2377630.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGNYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            complement(64963..66897)
                     /locus_tag="ONT85_000635"
     CDS             complement(64963..66897)
                     /locus_tag="ONT85_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCR2377631.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            67178..68134
                     /locus_tag="ONT85_000636"
     CDS             67178..68134
                     /locus_tag="ONT85_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HCR2377632.1"
                     /translation="MEWWGIKMKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSES
                     STAQSSTAKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKY
                     DNVNENGESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSS
                     ESFQNYAQQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGK
                     VTESNFDYIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPAN
                     VEVVTGATHSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            68224..69327
                     /locus_tag="ONT85_000637"
     CDS             68224..69327
                     /locus_tag="ONT85_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HCR2377633.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            69340..>69518
                     /locus_tag="ONT85_000638"
     CDS             69340..>69518
                     /locus_tag="ONT85_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HCR2377634.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAIN"
BASE COUNT        21190 a        14021 c        11826 g        22481 t
ORIGIN      
        1 aagcttacgc cgtctttcac tgcaaagact gttgttccac tttcaatact tgttccttcc
       61 cctgcggtga aggttacttt gtggtagcct gcaggaatgt tttctcctgg attttcataa
      121 atatctttac ttacagttac tgctaaatcg gtcgtgccta cagaaccatc ttggtaaaca
      181 attttaatag gaattgttcc agtaatcggc tccgattctc ctacaccttt agctggagtg
      241 aatgtaacaa taccagtgtt tgcatcaaca cttgcattac cgaagtactt attatagaca
      301 tctgaaattg aaaaggcatc tttagctaaa ggaacatctg gtttatcaaa tgtaggtttt
      361 ccactttgaa ctgtcgtttc tggagttgtt gttataggta cgtatgttgc atcattttcc
      421 atagcttgag aagcttttac agcaaactct acatccacgt cgaaagttcg atttccgatc
      481 ttaacagtta caataccttt cgtactatca gtaacgttag aagtatcagg ctctgttttc
      541 catgaatact ctgcatcttc aggtaagttt ttattattcc gaattgattc tttagcatct
      601 ggtttaaatc caactaccac ggtttgttta tcaaccgctt ttggtgattc cttaataacg
      661 gttgaacctt cttctggttt atcaaaacct ggagaaacga tttggatttt taattcatct
      721 ttcgcgatta atttacttga atctacaccc gtaaattcaa attctacagt atctgaaatt
      781 ggagccccat ctttttcatt tggagcgata gttttttctg ctaatacagt accttcttta
      841 tcaacaagtt gggctttgtg acctgttccc gctaactcgt ggatgaatac tttcccctta
      901 actgttgtgt caacatcata tacttcttct aattcaggtt tcccgatata tggattcgtg
      961 tctccgctct cttctttatc gtcagggttt ccatcaccat caatatcttg caaataataa
     1021 taacctgttc cataagtgtt ctggattaat tttttattcg tatccgacaa ccaagaatca
     1081 aagatgaaat cttcaccaaa tggtttattt tcttcaactt gtgctgttgc tttcatatct
     1141 tctaagattt catctaatgg cttagtcaat tgtaaaacat atcgaataac gactggacgt
     1201 ggacttgcat tagcaaaatc gataagttta gttaaatcat tattagttat tgaacctatg
     1261 ccataatttt catgaccttc ccgtcgctcg attgctaaat cagctacttt tgttccggat
     1321 tcaaacgaaa catcatatgt tactttgtca gcctgaacca tgtgcatctg tatacccgct
     1381 acatatggaa ctaatcgttc atcaattttg taattagcat accaaggcgt gccctttgcg
     1441 gaagatgaat atgagaaatt ataatttttc gttaacttat gatctaagat tatagctcca
     1501 ttagcacctg tggatcctga accatctttt gtttgagtat taggtttctc atattttgca
     1561 cttactgatc catgtttaaa ctggtcagga ttattttcag atacatctaa gctattatag
     1621 aaatcatctg aatctgttaa aaagtagccc gtactatcaa tacccggtac aattttatca
     1681 tcgtcttgat gacttaccaa gtagattcga tacattaaac gatcggtctt gagattgtct
     1741 atgcctatct ctttaaaaat ttcatctagg ctcttttcaa acgtaattac cccattagga
     1801 gcggtttgcg tatcggttgt ttctgcccca gcaaataatc catcacttgc acgaatataa
     1861 ttgaccttcc aaatatttgt aggtctaccc agcgaatcat taattcttac aaaggtccgt
     1921 ctagaagatc caattccttt ctttggatcg acctgaattt tttctacata tttagctaat
     1981 cgttcatcta attgaagacg gatttcccag tttttagttg atgctgcaat attatgtttt
     2041 ccgtaaactt caaatccaat tgtattgcct tttagtgtgt ctccagttag ttcttgagga
     2101 ctaaattgta aatccgaaaa gttataattt ttcgtataac caggattctc ctgaaaagta
     2161 ttccccgaag ctactttgtc acctacttgt acggagctta tcgctgtttc aagtgggctg
     2221 cttggagaaa tttcatcttg tgctttaact agttcagttg ttcccaagac aagaccaaca
     2281 ccaatcaaaa cagatgcaac cccaacactt aattttttga tactgtactt caatactcgt
     2341 ttctttgttt tttctagaaa tactctctta ttattcttgc taaccattat tcaaatcaac
     2401 tcccttaata attactttcc tagtaagaat aaaagataaa tctgatacgt attattcaca
     2461 gaataggaat cttactaacc ttatgttaac taatttactt agagaatttc atgaacatta
     2521 caacaaaaaa agtacatttc ttcatgtttt tttcggatat ttgtgctttt taaccattca
     2581 gcagctgaaa acatataaaa agatatgtat gtattctttc tgaaattaag ttcatttcta
     2641 tgcgatgaag catgttttca aaatattacc ctttcttaga ttaaacttca gagtattcaa
     2701 ataacggaat aaggagcctt atcccgcatt actgaatgag actgtgacat aagtaaaaag
     2761 gtgacttcaa attccttctt cggaatttga aatcaccttc tttataatta taaagtatcg
     2821 gtttcacagc atttctgaat gattcctaga atcttaaacg aatgattgga gttatcaaca
     2881 ttttttcact cgtttttagc gaaacaaaaa atgacatttg tcacagcctc attaaattat
     2941 tgattctttt tctttataat tgaattgtcc atatttaaag tgtctagtac tctacatgaa
     3001 taattaattg ctttcactca aatacttata atctattaac ttgtagttgt ttaacttttc
     3061 tgaaaaagtt agacccacgt aataatcttc ccagctttct ctttcaatta actgatccaa
     3121 tttttgataa tccataaatt catcatgtat caaaaagaat aaataacttg atattttagc
     3181 atctagctta atcagattaa ataatgtttg tttaaaattg ctaccattaa tatgactgat
     3241 agattctcta gcttcttcac ataaactttt tactttatta attactactt tgtcatattc
     3301 atttaacaca ctattattac cattacttag acgctcagat tctacgaaac aactcaaatc
     3361 agaaatttcc caaaaaaatt ttttgttaaa atccaatttc ctcaactcct ccaaactacg
     3421 taaatgatat agtctcataa taaaataaaa cctctcagct aatgttgttg aaattgtcca
     3481 ctaaacctaa ttgatattgc ttaggagata cacctgcgta ctttttgaat actctcgaaa
     3541 agtgtgaaga atctgaaaat cctaaaccta aagaaacttc actgatagta tggtttgatt
     3601 tcaacattat tttagccgta gaaatttttc gcttagagat ataattataa atcgtaatat
     3661 gcatttcatt cttaaagctt tctcttaatt tagtttcact tacagaaaaa taattagcta
     3721 tttcctttac agtaattctt ttatagatat ttaagccgat gaattgaacg acagaagaaa
     3781 ttaagggaga aagatgctct atcttagttt ttccgatttc cttagtataa taaacaatcc
     3841 cagaatccct cacttttaag acctcaggta acgatacagc taattcgttt tcttgtatca
     3901 aagcatctct actttgatac gcttctatta tatccattcc taatgttata gacgtttgtg
     3961 ctaacttttc aaaaacaatg atagaataat ttttttcaga ccgaatcgta tctccactaa
     4021 cacttggaat tattccatta ctcagattaa aaaccatgtt ttctaaattt cgaatatctc
     4081 catttttaac atattccagt attttatttt cataaagaaa cagatatatt tctggatcgt
     4141 aattttgtga taacagatta tctattttga tttgctcgat agttttactt agattttctg
     4201 tatattcaat taacggttca tggaatagat cttctatttt tccagtaaaa aaataatgaa
     4261 ctaaaatcag aatatctcga atgtctccta aagaatacaa agtcaaattt tgcaatgaat
     4321 tatagagtcg ttctctatca gccattgcaa cattcaaatt gttcattttt agtttaaaaa
     4381 aaactttatc aatattattt gctctaaatg gaccaaataa gtaatatctg gttttaaaca
     4441 tgtatagaag aaacatctca ttaaaatcac cagtaaagaa atggaaatct ttagtttttt
     4501 taatattatc agttagtaac tttttataat cataaaaaaa ataagtagtt ctatctgagc
     4561 gatactcttt tataatactg aattcggaat taaaaaccac gataggtaac tttaaacaaa
     4621 catgaagaga ctttaataag acttctttat ctgaaaacac ttttgtttct ctctttcctt
     4681 tctacatcat taaatagtat tttatacgtt atctaaaata ctattcctct gtcaaaataa
     4741 gctaacttac ttcattataa aatatccact tatcgctttc atgtatattc caccgtaaaa
     4801 agtacatttt atcaccgaga actcatcatt tttcatcatg atgaatacta tttacatata
     4861 tctagttcaa agacaataat tattatgatc cgttattatc taagcactat acagataata
     4921 ggaatttttc gtttctaaat attccaattt gggaatccaa taaaaatttt cgttcaaata
     4981 cgtgtctatt cgaactcttt tctagttgtt aattatgaag ggggaagaaa taaaaaatac
     5041 aatctataca cgaataagaa tattatccaa aaggaggttt tagaaaagat ttattatacg
     5101 taaaaagctc aagacatttt cttagccata aggacatgtt tgatatttaa gaagatatat
     5161 gaattttctt ttctaagtat aaagataact atccattatc aactaaaaaa acatttttga
     5221 aaataaacat cagcagtcct atcaatgcat gtgagcttat agaagttatt cgcagcgcag
     5281 cataattgca ttagcttctt ttcgtgtttt ttgcctcttt tccctttaaa tcttaatgga
     5341 ggaggtgtgc aatatgtcag ataacgacga acgcgtaaat gcccagttga ttcgattctt
     5401 tgaaaaggtc attttgaaca ccgctaatac ttattataaa aaacaagaag aaattcaaaa
     5461 acatgaacaa cttgatcaac tcccaccata tttgtttact aaagatacta taaaatttaa
     5521 acaaccgatt aatgttttaa atgtgactct ccttgttgaa gatataaaat tagcaaaaat
     5581 ccttcctttg ttgaaagaac ctgaacaaat ttttcttatc gaaaaattta tctttgataa
     5641 aactgataaa gaaattggaa aggttctggg gattacaaga cagggtgcga caaatttaaa
     5701 acatcgactt tacaaaaaaa ttcaaaaaat gctggtttaa tatctgcata ataactaatt
     5761 ttgtatgctt ctttgctcgt tctcctcctt cccaaagaaa tagattataa gtccatggga
     5821 agaaggtaga tttatgaatt ctttaaaaga agatttcatt ttagcaaagg ctggaaatga
     5881 agaagctgtt gaggcaattc tcaaaagatt tagctctttg attcataagc aatcctggag
     5941 aactggaaaa tatgatcaag attgttatca agaatgtatg cttgcaatat atctagctat
     6001 ctcgaaattc gaaattaaag aataactcaa ctaagggagt ctgtgacata agtaaaaaga
     6061 tagtttcaaa tttcgaacaa taaatttgaa actatcttct ttataattat aaagtatcga
     6121 ttttacagcg ttctataatg attcatagaa tctaaaacga atgattttaa ttatcaacgt
     6181 tttttaattc gtttttagct taaaaataac ttttgtcaca gtctcactcc taaaattact
     6241 ttatttacgt atgaagtggg caggaggtcg aatccagaac tgggctatgg tacttcaacg
     6301 aggacttctc ctctagaatt gaaaagcatg cgatttacct tccgtaacga cggtagattt
     6361 attcaactat gtgtccaatc aaaatttgtc tgatttcaaa aatagtcaag agaattttgg
     6421 ttgttaaaaa ctcttatcta tcaacttcca cagtttagtt gacaaaccct gatactaagt
     6481 tgggctatga tacacttttc ttgactatca gttacgttac cggaaggaat gaactctatt
     6541 gagaaaaaca tatgatcgta aatttaaact aaagatttca caggatatcc ttgagaaaaa
     6601 agtaactacc aagaaaattg ctgaagaata caatatctct cgtccaacaa tttcccgatg
     6661 ggtttcagaa tatcgtcgat acgggaaaaa tgcttttgct ggacaaggaa aaagattacc
     6721 tgataaagca gatttctaca ttcttgaaca ggaaaataaa cgactaaaag aggaagacga
     6781 gtgttctacg aacacaaaga tcgatacgga tctccaggga tcactcaaaa actttacgat
     6841 gaaggaatcg aaatcaataa acgtgttgta gctgttctta tgcgtgaaat gaatttatgc
     6901 acaagaggat ttcatcgacg caaatcttct catggtagag aaaaaacaat caaagaaatg
     6961 gtaaaaaaat gttgaatcga taatttgaac agaaccaaat tgatgctgtt tgggtgatgg
     7021 atattactta cattccatgt aatgacggtc gcctctattt gtccacctat attgacttag
     7081 caactcgtat tctacgttac tatatggtag attcacacat gaaaaaagag attgtcgtcc
     7141 gtccattaca aatttattaa ggagaattgc ctacggtaat tcattccgat agaggtagcc
     7201 agcatcggtc ttttatttat caagagctgt tgaacgacca ccacatcaca cattcagtga
     7261 gccaaactgg gactcctgtt gacaatgcag taattgaagc gtttcatcga tcaatcaaat
     7321 gagaattaat cgactccaac aaatataaaa gtaaaataga gatgaaggtg ttaatccaag
     7381 attatttaga agactactac ccaaacaaaa gaatccatac aaaatttatg atgactcctt
     7441 gacaatttga agcgaagaaa ataagggatc tataatatct agtgttggtg acgccttctg
     7501 ttgccaatac tttttctttt ttctaataaa cgcaaacaga caccttaaaa tcctttagaa
     7561 taaagtcgac gaaaaccatt caaaggagtg tttcaagatg tcctattcag agtttaccaa
     7621 agagttacta gatattctag acctaaatct tatctttcat gaagatgcct ttcgcaaaga
     7681 gcgaatcaat gacgaaacat gttttgtttt tgatggcaca ttgacctatc aaccggagga
     7741 atgctttcat cgccactatc aaaatgaaca aacgatcatt aaatagggct ggaaaaaagt
     7801 atccatctta ttgaatgatg tcagtaacta caagaccatt ctgcggatta acaaacagcg
     7861 cttcaaatgt aaacactgtg gaaagacttt tttagcagaa gattcagtta gtgatcgtcg
     7921 ctgctccatt gctcgcagag tcaaacaagc cattcttgag ctattaagcg aacctctttc
     7981 tatgtctttg atcgcacgta tgaagcacat ttctccgacc actgtcattc gaatacttcg
     8041 cagccttcgg cctaaaacca tttctttgag ccagctgtta ccagaagtgg tgtgtttcga
     8101 tgaattcaaa tcagtgaaaa atgtctcggg agccatgagc tttgtcatga tggacggcaa
     8161 gacccatcaa ctgattgata ttgtggaaaa tcggcaactc aatcctttac gcgactattt
     8221 cttgcgctac cctataaaag tccgtgaaca agtgcgtcta gtggtttcag atttctatac
     8281 accctatcgc acattggtga aagagtgttt tccgaatgcc cgaatcgtcg ccgatcgctt
     8341 tcatattagc caacacattg gtcgggcttt taccaaccac cgaattcaag taatgaagac
     8401 ctttaaaaaa ggggatcgtc ggtcgaagca cttaaagaaa tactggaaac tacttcaaaa
     8461 gaatgcgtgg gaattgaaag gtcagcatcg ctactggcgt ccgtcatttc gagatcatct
     8521 gacagaagca gagatagtcg atcggctatt atcttatgat gattccttaa agcgaggcta
     8581 cgaagtctac caggattttc tttccgttat cagaagacaa gatgttcctg aattcgtcgc
     8641 gctcctaaaa gaagactaca aggagttgcc agagcactac caaccagtgt tcacgacctt
     8701 caagaaatac caaacagaaa tcaaacgagc gttgcgcgtt ccctattcca acggccctat
     8761 agaatgtttg aataaccata tcaaagtact aaaacgaatt gcctatggct atagaaactt
     8821 tcaaaactac cgagaacgaa tctttttata tcgaggaaaa tattttaaga aaactaagaa
     8881 tactacccaa ctgactaaag ccagaacaac aacacggcta gacaagatag ctgtctagcc
     8941 gtgtgagtat ctcgattatt ctattgaatt tcttcaccaa cactcattga cgaagagcca
     9001 aaataagtaa tctaaccgac tgttaactaa accattgcag gtcccgttaa agatacttgt
     9061 gcctgttgtc accgcttctt tttcttgtcc acaagcgctt aacacactta ataaagcccc
     9121 tgctattatt aataataata tctttttctt attaaaaacc tcctcatcta tttccagatt
     9181 agtaaatgta gtaatctgtg ctttcttttt tctcaatact aataactaac ttatggggta
     9241 aacaaatact cgtttgaccg ggttttgaga tccatccagt tctaaccgcg atttgatcag
     9301 gactattatc ttctttaact cgaattcttc catctttaat ctcaacaata ttatattgtc
     9361 ccttcgcagg atgataattc acgagtttat gattgatctt atccaaatcg aagcgatcca
     9421 tttccttgcc atcaattcga acgatggcat atttgatagc atcttcagtt gtttcattct
     9481 ccttttctat agagaataca aataagggaa taaaagaggt aataatcaaa atgccaataa
     9541 ttatgacatc gcctatcttg acaccagctt ttcttaaaaa tttcattaac gatatctggc
     9601 taaacatatt agactttttc catggaaatg gtgaaatttt tcggaaatta tctgcattaa
     9661 gagaatcagt taaacaaccc tcaagttgtt ccttaataca ttgttgatcc atcttcatac
     9721 caataaaaac tagtttatta atgcgttcac caaacaacgg atgccaggct tccttcaatt
     9781 cgggattttt ttgtataatc atttgacact caattttagg taaactagct acccaataat
     9841 ttaatgattc aatactttta tagctacccg atgtttcaaa gatcgtcgaa aaatcactat
     9901 gttgggcaaa ccaaagaaac ccttttgctc ttgtaatatt gccaggaaaa tttttatccg
     9961 taagatgggc aaatttttgt gcgtcaagga gtaaccgatt tcgatagata aaacttgaga
    10021 taccatattc ttctgtttca ggaatatgat tttgatagcc aagctctaac tctttgaacc
    10081 agcccactga ataggaagtc tcatctatat taaaaagatt tgtatttaga attttcgata
    10141 agtctacttc acctttaatt gtcctaatta ttttggcatt ggactgtaac ttagcaatta
    10201 gaccgttaat tttgtaaatc gtcttggttg acactaagtc actattattt aaaagtaata
    10261 tgttacaaca ctcgaattga tcaattaaca agccacgaat atcctgttct tcttcaacta
    10321 cattaccttc agcttttctt tcctgaatct tttcacccgt ttcgaaatta tcccaaaaac
    10381 gttttacatc cactactata ataatggcac caatctctaa tcgttgggtc aaatcaatgt
    10441 ctaaagggtt tttagctaat ataattgctt gaataattgg aattggtttg ctgattcctg
    10501 aagcttcaat aacaatttga ttcaagtcag aaaattgcac taattgatct agttcaacaa
    10561 tcaaatcttc gcgtaatgta caacaaatgc atccattcgg catcgaaaca agtttttctc
    10621 cagttctttt aaaacctttc ttgtcgacta ataccccatc aacatttact tcccctatgt
    10681 catttacaat gacagcaatt tcctttccgt gatcaccgta cagaatatga tttagaagta
    10741 tcgtttttcc tgaaccaaga tagccagtta agattgtaat aggtattttt ttcataggga
    10801 acctctttct ataccttgaa tttacacttt aagtgcaaca cataaaaatg agtcataatc
    10861 gatatccaac tagtataagt ttgtcgagaa cactcctaga aggaaaataa ttatgagcca
    10921 gtatcgtcat cttactatac tcgaacgaga aagaatcttt ctgcttcacg aagaaggatt
    10981 ttctattagt ataattggca acgacattca gcatagcccg tttactattt tacatgaact
    11041 gagaagaaac aagaaaaaaa ctattcacct tccaaagcgg aaaaaaatta tgcttatcga
    11101 agaaaaaaat gcggacgtta tcacattcta aatgatccgt gagtttggtc aattgttcgt
    11161 catttatttg tcgattttca gtggtcgcca gaggaaatct cgaatcgttt gaagcatgaa
    11221 catatggact tttcaattag ttttatcacg atctaacgtg caatttgcaa tgatcttttc
    11281 gatacagaac ccctctcaca cggcaataaa ggcctgatcc gttcattgca ccatcgagga
    11341 aaaacaagac atacaaaaaa tcatgtcgag tctcgtggaa agatttgcct tactcattcg
    11401 atccatggcc gtccaaaatc cgcagatgat agaagtgatt ttggtcattg gaaagccgat
    11461 attgtagctg gcaaaactgg tggtacttgt ttagtgactt tgacagacag aaaatcatgc
    11521 ttccttttag caggaaagaa ggtccccaga aagaaagccg cttatgtgag cgagaaaatg
    11581 attgaacttt taagcagtat tccaaatgag gcggtcaaaa cgatcactcc agatagagga
    11641 aaagaattct ccaatcattt cgcagttaca gaagcattga ataatatgcc cttttatttt
    11701 ccagatcctc atgcccttgg caacaatgaa caaattaaaa tacgaataga ctcattcgag
    11761 aatacttgcc aaaatcgaaa gagatagcta cggtagataa ctcaacgata aatcagatga
    11821 ttgaaaaact gaataacaga cctagaaagt gtttagattg gagaacacct tatgaagtct
    11881 tctttgataa agtgttgcgc ttgatttgac aattcaagac ataataaaaa gcagatcaaa
    11941 tccaaataag ggaaaaatga tctactctgg tattatcaat taattggcca agctattatg
    12001 ccgttttgct aaacgtctac ttcttccagt attgaccttg ataaaacaac aatcaggatc
    12061 ccgttttaat actctatgaa ctgtgtcaat actaacatgt aaagttgcag caatatcagc
    12121 taatgtactc ccatttaatc tcatttgctt tgcaaatcga taacgccgat aagcatcaat
    12181 attcttggaa tccatagaca ataatacttt tcttaccatg tatttcctcc aaaaattgaa
    12241 ttttaatgag ccatcatttc ttgagcaatt tcatgtttag acaatgaagc tggatagaaa
    12301 gtaggccaat tatgcaattc attatagagt ttttcttgac tttctccacc aaagaaaata
    12361 tggaaatgct ctgccttact tggtgcaatt ccatgatcac taaattgaat atatttaaaa
    12421 cgaccagcac ctgcatcatc agtttcaaaa ttaaaacgaa caccacggtt tcctttttca
    12481 taattcaaaa ttttgtatcc aacatatttg tatgtgtatt gatgatgttc accgttgact
    12541 acaaaatcaa tggtgttgtc tttaatgttg atagtatcaa tatctgtctt atagccagta
    12601 gtataataat ctttgtactc ggcagctgtc atgtctttat tgatttttgc tttgtaatcg
    12661 aaaacttcat ccaaaattcc atcttttaac aacggataaa cagattgcca ttcgcctgta
    12721 taatcactca attcgcgatc cttcacatcg gcatctttaa agtacccatt agcaactgtc
    12781 ttttcatcct tcgcttcttt ttctggaact acctcttcac cagcaaccat agttgttttt
    12841 tctaacgctt ttagattgtc ttccattact gaaacataat cttcaccatt atccatttgt
    12901 tcttttgtaa gactttcaag aggattcagt acttccaatt ttatcccagc ttcgttggcc
    12961 aaagtttttg caattttatc attagcattc ttttcaaagt aaatataatt gatcttattt
    13021 tttttaacat actcttttaa ttcagctaat cgaccagaag atggctcttc ttcaggactt
    13081 agtccagcaa taggaacttg tttcaatcca taatccaaag ctaaatatcc aaaggctgcg
    13141 tgttgcgtca caaaactttt ttgtttcgct tctttaagac tagtggtata gtcagcatcc
    13201 aaccgtttca gtttagtcaa atacttttca gcatttgtct caaatacttt tgcttttttc
    13261 ggatacaatt taactaactg atccttgata tttgaaactt ccttaattgc cattttgggt
    13321 gaaacccacg tatgaggatc tagttcatga tggtgatctt cttctccgtg atcatgatct
    13381 tcttcttcac tacctggcaa taaaatcatg tcttttgttc cttctactac attaggccca
    13441 gctttcttcc aactttcttt agcttttggt acccatgatt ccatgttttc gttatgatat
    13501 acaaaaacat cagcatcatg agcttttgcc atatccttag ctgagggctc atagtcatga
    13561 ggttctgatc ccgctggaac cattaaatcc acgttcgctt catcgccgac gatgtttttg
    13621 gtgaaatcat acattggata gaatgttgtt acaatttcta attttccatc tttatcattc
    13681 gatacttttg catcattagt gctggagcaa gctcccaata atcctagagc tgctatccca
    13741 ataagtccta cactgatttt cttcattctt tttccttcct taattatgct aatttcttca
    13801 ttttatttcg tttatgttct ttgataattt ttaatgccct ttttctagtt ttaatattcg
    13861 ggcttttaag tcgtcgatat gcactagata tatccgtttt ctccattaga aattaccccc
    13921 aatcactact tatcattatt tgctttccgc cagtaattta tcaaaactct gtttgatcct
    13981 ttcttcatcc aacatttttc cgattagaat aatagtggtt tggtcttttt gtcgagtggt
    14041 gtattgaaaa ttaacctgtt cgtttacctc ttgcatggca actataaaat cctgctcttg
    14101 taaagcaatc aatcctttaa aacgatacag gctcccttgg ttggttaaaa tcagccagtc
    14161 aatccattgg agtaaaaaat ctttttttat gtcttgatct gaagttaaga gaattgattt
    14221 aaactcatgt acttgatggt gttgatgata gtatttgcca ttatagtgat tttctgatga
    14281 atcactttca aaaactaagg gatgattaaa ttttttcaaa ttataaaaat cctttttttg
    14341 aatcgtttca ctatgtgtga acattcgaat atcggataaa gggttaatac ttttgatttc
    14401 tctctgtaat ttttttagtg cagataaatt caagttttgt tttctagaca caaaaatgcg
    14461 atctgccatg acaagttgct taagggcttg tggttctctt tcaataagtt caaaactgtc
    14521 actatcaata acagtcaata aactatcgat atatactctt cctcttagtt gaggcgctgt
    14581 taaaagtgtt tgaatgattg gttggggatc tgctatacct gtagtttcaa tgatgatttg
    14641 tgaaattggt tgttgatgtt cttctattgt ttccagtaaa gaaatcaata ttgctaataa
    14701 atcactgcga agactgcaac acatacatcc attatttagt tgtaacaccc gttcctcgct
    14761 atgaactaat aatttgtgat caatcccaac ttcaccaaac tcattttcaa ttataataat
    14821 ttcttccctg gggtagggtg agacttgcaa ggcttgattg attagcgttg tttttccagc
    14881 ccctaaaaac ccagaaacga ttgtaactgg tatgcccatt ttgtcacctt tttaccactt
    14941 agctatttca cttctttaaa aattgcccgt ctgcgcaatt ttggcgaata cttttttaat
    15001 tctaaccgtt cagggttatt ccgtttattt ttgctggtga gatacagacg ttctccagtt
    15061 tcgacacatt ccaaaataat attttgacgc ataaatcttc ctccttcaat acttatcgat
    15121 cttcaagata tgcttgaatt tgttgtgcat cttcaaaagt taactcgcca gattccaatt
    15181 gtgaaactaa attttctttc atatcaacag gtatctccca ataaccatcg atacattgaa
    15241 ataactcctc gtcatactct tcaaaaattg gatagattct taaaaaatca tcaaatgata
    15301 agccttggtc ctcatagtaa tctttgacta atccattttc aattgcctgt tgggtcggta
    15361 aacccgtttc agtaataatt cctctctcta ccatcgaacg ataaaaagtg catttatcta
    15421 gcgattgctg ggtcgtttca agataatact ttaatccttc ttcaactaaa ggtgggtaaa
    15481 taatttccat tttgattccc tcctaaaagt tatatattaa actattttga agctccttta
    15541 attactttct ttccattata gtaccctttt aaagatacat gatggctacg tctataatca
    15601 ccattcgctt cgtcaaatga aatttctggt tttactacag tttgatgagt tcttcttaac
    15661 cgtttcttcg ccttagatgt ttttcttgct ggtactgcca tgatatttcc tccttttacc
    15721 aacttgcttt ttttactcca ggaatcaacc cttgatgggc aagttctcta aacttaatac
    15781 gggacatacc gaactttcgc atataccctc ttggacgacc atccgtctga tcacgaagcc
    15841 tcagacgatt cggatttgaa tctcttggca atttagcgag agcggtgcga tctcctgcgg
    15901 cttttaattc ctgacgttta tctgcatact tttcaatcat cgccatttgt ttctttgctt
    15961 tagcaatttt cgactttttt gccatatacc tttatactcc ttttattttg atttagacac
    16021 ctcaaatcgt aatcgttacg ttttgacact gatactttac caacaagagt tgctcttgtc
    16081 aacaataaat aacatgaaat aaaactcata tgaatagata actatttgaa aacgaattta
    16141 tcggtacata gagataatca aatcatttaa aagaatatgc agtttaggct ctgaaaaata
    16201 agaattcact tttattagtc caatcaaaca caaataccgt tttaagtagc ttttcctcta
    16261 cttaaaacgg caatatgaaa gaaactataa ctttttattc aatcttttat gtttaactgg
    16321 tttatcgcca attttttaaa actttgatta ttccttatat tataagaata gacattaagt
    16381 gaactaattc aatcatactg attctttcgt gtattcaaaa ttagatgctg tttaataaaa
    16441 ttattctttt tgaaaaagaa ttataactat atcccaaaaa ttgtaggtta atttaaagcc
    16501 tatttcatca tcgtgattga gcttcttttt catagacgaa tttctcagat agtttagttt
    16561 tctttatgag atttttgtat atttctgacc ttcgatacaa ctcattatga gtaccttgat
    16621 caactagttt tccttgatcc atcactacaa ttttatttac cttttcaata gacttcaagc
    16681 gatgcgatat aattagtact gtcttatctt gtattaaacg atttaaactt tcctgaatac
    16741 gtttttcatt ttccgcatct aaactggcag taatttcatc taaaattaaa attggcgcat
    16801 ttttcaaaaa agcccgtgct atggataggc gttgtcgctc tcctccagaa agtgaacttc
    16861 cgttttctcc gaccaatgta tgataaccgt caggtagttt ttcaataaag tcttcgcagt
    16921 tagctaaacg agctgctttt ttcacttcct catctgacgc ttgactattt ccaatacgta
    16981 tattttccaa aatacttgta ttaaacaaag ttacctcttg aaaaacaata gcgattttgc
    17041 taaacaaaga agctggagat acacgtttaa tatcataccc atcaattaaa atacagcctt
    17101 catcataatc atacagtcta gacactaatt tcaagatact agttttcccg gaaccactag
    17161 cacctactaa cgcagtcact tctccttgtt tagccgtaaa agagatatga tctaaaattg
    17221 gggtattgtt atcatatgag aaagagacat ctcgcaactc aatgtcaaaa gatttcagag
    17281 gactatctga tccctcttgg atactggttt ccttcatagc tctaattcgc tgtattttag
    17341 gagctaaata aaatatttca agcactgctt ctttcataga atcgaaggaa tcctttattt
    17401 ttattgcggc tagtaagtaa ccaacgacgt agagtatagt cacttcaccc gttaacagta
    17461 agtgaacacc tactagaatg actactgcta aactaacata tgagaaaata gaggataagg
    17521 ccatgactga aaaagtactt aattctacct ttaaatgaat cctttcactc tcttccattt
    17581 ttttgaaaag tctgtcttga acttttttag atagattaaa gctattaatc tcttgctgca
    17641 attctatagt ttcttgaaat tcttccgagt tttctctcaa agtatcgtaa tatttagtat
    17701 tggctttcgt ttgggatttc tttgataaca agattaagac aaaactaaat aacgttggca
    17761 aaataacagc taatcccatt ttgacattac caaccaaaag catcactgaa ataaaaggga
    17821 aaaacagagc cataccgccg gctttaggta ttgcatgact catcgcatgc tcaatacctt
    17881 caacgtcaga catgatagtt tgagataaat ctgataaatt atgttttgaa aaataggata
    17941 atggtaaatt tgacaaatcg tctgcaattt gcactcttaa atgcgcactt tctttataag
    18001 tactattata taagttttca tactccctat ctaacaacca atacatgaaa agcaaagtaa
    18061 caaacgatac ggcaaaataa agccaacggg gctttgtatt ttctaaaact aattcgtcta
    18121 tcagcatcat tataatgaat gctggtacca tattaatgca gtatacaaaa aaactagaaa
    18181 tagaagcttt agttaaagct tctgcgccct gatcacttaa agcaaatttg tttttataaa
    18241 aacttttcat tgtcaagcct ccaatcattt gcattctcat aattagtgac caattgttta
    18301 tacacttgat gtttagccat taattcatta tgatttccac gttctactat tttcccttct
    18361 gacaagacaa gaatttcatc tacagattta atgctggata atcgatgagc tatcataata
    18421 actgtttttt ctttcatcag atgttgaaaa gccttttgta gttcatactc attatctgga
    18481 tcaatagatg cactggcttc atccataata acaatctttg catcttttaa aattgctctg
    18541 gcaatagcta tacgttgttt ttctcctcct gacagataaa ctcctttact accaattaag
    18601 gtatgctctc tttcgggaaa tttttctaaa atcgattcgc aaccggctaa tcttagcgcg
    18661 tcaaatactt tacttgctgg cgcttgagga tttgctatag aaacgttatc gtaaatactc
    18721 tgattgaata gtttcgtatc ttgaaagaca aaagcaattg tgttaataat tgtgtctttt
    18781 gaatagtgat ttaaaggctt accaccaatt ttaatctgtc ccttatccac attgtagaaa
    18841 cctgaaatta gtttagctat cgtactcttt ccgcttcctg attttcctac taatgcataa
    18901 attctttttt gttctaaaac taagttgaaa ttttctaaaa cacactcttc tttgtatgaa
    18961 aagctgacat tttcaaatgc aatgtcacaa tttttaatct gtgtctcgtt cccatatttt
    19021 agttggtctt gcttcatttc taaataaagg gagtctagtg tttcgactgc atatcttgct
    19081 tcaaacaagt gcatacttac atacattatt ctcatgaaag aaacaaaaat aattccactt
    19141 aaaaaaaaca acatcaacaa gtcaacagct aatatcttgg gagtggtaag agaagttata
    19201 aaaaaggtta taggaactat caaaatgcaa actatgccaa aaaatagcca ttgaaaaaga
    19261 acaaaaggtg ttttcccact caaagaatac ttatatgcaa acttagcata atcgttaatt
    19321 gctttattca aggctttaaa tgatgttact ttagcaccaa atatctttat aacttgaatt
    19381 ccgcggatat attctaccgt ttcagagctt aacacatcta acgattcttg atatttttga
    19441 ataaaatttg tattctctgt cattttcttt aacaagaata caccaattac taacatggcg
    19501 attagaacca atccaatttt aaagttaatg aaaaagccta aggcaactga taaaacaggt
    19561 gttactatgg cttgagcact atctggaatc aagtgagcaa ttgccatatg tgttttagcc
    19621 gcgttgtcat caattgtctt tcgaatatat cccgaagaat ttaaatcaaa aaaacgaaaa
    19681 cttgcttctg ttaaaccgct aattcccttt tttctcaaat tagtctctaa gcgaaaccca
    19741 aggatatgag aaaataagcc agaaaaaata taaaataaag ctcctaaagt taaccaagca
    19801 acagtttgaa ccgcataact cttagctaaa tattcatctc ctacaataat tagagactga
    19861 agaaatttgt aaatactgta atatccatag actgttaata cgacagaaaa atcagaaaat
    19921 aaagtggcta accatccgta gtatttcatt ttagggacat aactaaataa ttttttgtat
    19981 actttcattt tttcctcccc cgcattcaat ttcttctctt attcgtttac aactgttaat
    20041 gctttccgta atagcgtaaa ttagagatgt acaaacagat aaaaggtaaa ctacgttcta
    20101 ttttttgtat ttttttaaga cttttcttcc aatcaataca ccaatcatag ataaacttgc
    20161 tacaactaac actgaaatga ttccccatat acctaccgca tagtggatca ttgcatccat
    20221 tgttgcagca gattgtcctt tatctatcca atcttgacga aaacgatctg taaagaacca
    20281 tataggaatc cactgtccgg cagcactgcc taattgcatt agagcccaag acgcaacttg
    20341 aggaattgct cgatcatatc caaatacaga tagaataata tcagctaaga tcgccatact
    20401 aatcgaactg ataaaccaag gtaagtacgc cccgcccatt aacgtaaaaa ataacaataa
    20461 aacaattgtg taaatagtaa aaattccttt tctagggtag ttatagctaa tataaacaaa
    20521 tatgattcca gataataatc caaaaatccc gggggatatg ctgtgaccaa acgccccacc
    20581 aatactacta accattattg aaaaaaagta aattactagg gaaataactg tagctaaacc
    20641 aataaaaata taatcttttg ttttcatata tattctcctc aattctattt cgttatgtca
    20701 ttaaaaatct gttctatcaa agttgaatct tctactctaa gttttatatc tttttttatt
    20761 ccaccatttt ttaaataaat aattctattg catgttttat aaagaaattc cggatcatga
    20821 gtagctataa ctactgcacc cttttctttt aagtctttaa taaggtcagc aatcctttcc
    20881 atattaatat aatccaaccc acttgtaggt tcatcaaata gaaataaatt agtttttgac
    20941 aatgaagcta tagaaaccaa taatctttgt ttttctccac cggataaatc aaacggtaat
    21001 tgatctttca ttttataaag ttccattttt tttaaaattt tttcttgctg atctttacta
    21061 actatttcat tccccagggc taactcgctc ctcactgatt ctgtgaaaaa ttggtaatcc
    21121 atttcttgca ttactaaaaa cggcgatgac aaattttttt tattttcgat tatctttcct
    21181 ttggtagggc gaataatctt catgagtatt cttagtaagg ttgttttccc cacaccgttt
    21241 ttccctacca aaccaataac atctccaact ttgacatcaa aatttatgtc ttttagaata
    21301 ttttcataag taatttcttc aacttttaat acatctgcac ttaccttttc gatataggga
    21361 acttcaatcg aactattaaa aatgtcaaat ttccttaatg cgttattgtt ttcagttttt
    21421 aacaactgaa tagttgtagt ttttacagtt ctatttttaa caaatagaac ttgatcaatt
    21481 aattctttca aatagtaaaa acgatgttca gctataacaa tagtaaatcc tttatttttt
    21541 aaatcacaaa gtaagtcagc aagtttctta gtcgctttga agtccaaatt tgaagacggt
    21601 tcatccaata taattaattt agagtttagc gataaactac atccaataga aattttctgc
    21661 cgttcaccac ttgatagcga aaaaatgttt ttatctaata tatttgaaag atctaataat
    21721 tcagctaatt cattgattct tttatccata tgctcttttg taaatccaaa gttttccatc
    21781 gaaaatacta actcactcgt tgtatttgtt gtaaagaatt gtcctctagg atcttgactt
    21841 acgtagccaa tattttttga tatactttct tgtgttaagg attgtagcga gttattttca
    21901 aataaaattt ccccaaccaa ttttccgtca taaagtcttg gaataagtcc attcattaac
    21961 tttaatagtg tggatttccc actcccagaa gaaccagtaa tcaatgtaag actgcctttt
    22021 tttattgaaa aattaaggtc aacaagcgct tgttccttaa catccttata tgaaaaagaa
    22081 acattgttaa attcaaacaa ttctttcacc tcaaatcaaa atacttaaga aaaaaatagt
    22141 aacggaaaac aggatgatat agtctaataa gctcatgttt gttttatagt aagaacttct
    22201 tttcacagga gattctatcc cctttgttat tgcattagcg gtcatttctt cagcaatcat
    22261 agttgcacgt ataagaacgg gaactaacag aaattccaaa tattgcagag gctgaaaaaa
    22321 agacaagcga atgtttctca tttttaaaga ttctttaatc attcttattt cagaacgata
    22381 agtcggaaag aatctgatca ctactgtaat acttaacatt atttttttcg gtaaatgtat
    22441 tttttctaaa gaggataata attcattgct agtatagaca ctaaccaaca accatgagat
    22501 cattacgacc ggcaacattc gaaatacaat atataaggat gtcgcaaaaa atatagtaaa
    22561 aaactctatc ttgatagcat caataatttt cattactaaa aacagaactg aataaatcaa
    22621 gatgaaataa atacatgtct ttattttacg aaacatcaat actaaaactg tagcaataat
    22681 gaaacacccc acttgaaata tatttccctt tcctaaaaaa ataatgaccg gaaaaaaaat
    22741 atttaaagta atcaaggtta acggattgat ttttgacact ctttcacctc caaaaaataa
    22801 gatcgcactg ttatcttaag ctgataaaaa tgcaccgtta ttcagataac agtgcgatct
    22861 tatcaaaaag cataactgag aacaattctc aatgcaagct ttttttaatt aactcagtaa
    22921 ttcaatccac ccaggaacag aaaattgata caatcttctg gttaatagca tagtcttttc
    22981 ttttgaatag ctatgcttca atatatcata gaatgtagcg tagaatgagt aaatgtagaa
    23041 gtggatttct tcttcattaa agactaaatt ttgattgatc attccgatag tctttaactt
    23101 atctagacaa cagactgtct ttttcgtcac aaatgatgta attttttcaa caatatcttg
    23161 atatttggtt ccagctgatt ggaataaaat caaatcgaat atacgtttat tctcaaaaat
    23221 ataattaata aatggatcta attcattgaa gttctctctc caacttttct ctatatctat
    23281 attttctaag tttaagaggt atttggtaaa ctcttcatta tacatattaa taagcgcatt
    23341 aatatgcggc tgaataattt cttcaaataa cgcttcctta gaagtaaaat gtttataaaa
    23401 agcacccgta gttacgcctg cggtcaggca cagttgcctt atcttagttt gctgaaatcc
    23461 ttctttttca aaatggctta gtccactttc tattaacttt tggtgagtta taatataatc
    23521 tctggtttct atgggtactt cctcctaata agatgaatta actttattat cttacacagg
    23581 acttgaagtt tcaatcgtta acgtttatta ctgaactcat tccttagagc taaactaatg
    23641 agaaagagca gaataactga tacaaaaagc aatcctacaa gaattaatag actcatctaa
    23701 atcattcacc ttttctaaaa taatccgaga aagatttaaa aaaattatat ccagctatta
    23761 ggataagtcc tatcataatc actccaatag ccattttctt atcctcccta aaaataaagt
    23821 cttttactta aaaccttttt tgtaatcgtc aataaatttt ttccctttat cagttaattg
    23881 aaatcctata tctaaatagt cctcatatct aagtttagcc agcatctcac ttacagatgc
    23941 tggactaaca cttagaactt ttgctaaatc attatttttt aatttctttt ttctttttga
    24001 taattcctga attgctagta aataaattag tatagtagtc gttttaattt gtaccatacc
    24061 taccctccaa attatttcga aaggccttca gcaattttat ccaggttcca tttcatcatc
    24121 gcatagtaac tatcaccatc ttgtcctttt tctgcaattg aatcagtaaa aatcgttgaa
    24181 tagataggaa tattggtatc ttttgatact gttttcattg gtctatcatc cacactgctt
    24241 tctacgaata aggagggaac ttttgttgtg cgtaattttt caactaagtg ttttatttga
    24301 tctggtgttc cttcttcttc agtgttgatt tcccaaatgt aagcagaagg cacattatac
    24361 gctttcgaga aatatttaaa gcatccttca cttgtaacaa tcattttttt atcattcgga
    24421 attgaagcaa atttagattt agcttctttg tctagagaat ccaatttttc aatatactta
    24481 tctagatttt ctttatagaa ttttttatta tcaggatctt tttccgctaa ctgtttttca
    24541 atatttttag cgtaaataat accgttttct aaatttaacc aagcatgagg atcttccttc
    24601 cctttctcac tctgaccctc taagtaaata acatctatgc catcacttgc tgcaaaatag
    24661 tctttgtttt cctctttgtt cgcattttta actaattttg taaaccaagc atttcctcca
    24721 gtttccaagt taacaccgtt ataaaaaatc aaatctgctt ttgaagtttt ttgaacatct
    24781 tcaggcaaag gttcatattc gtggggatct ttcccaatag gtacgatact gtgtaaatca
    24841 attttatctt ttgctatatt ttcggtaata tctgctaaaa tcgaattagt aactactact
    24901 tttaattttt cgttcgaagc cactttatct ttgtctttac tatttattga acatgccccg
    24961 aatataaaaa gagcaatcaa aatccccaga cttcccagta ctgctttcca ttttctcatc
    25021 ataacgatct ctccttcttt cttaaaaatt tttgttttgg tgagattaga aaactaatta
    25081 caaataagaa tgccgctgtt aaaacaatac ttgatcctgc tgctatgtta aaactataac
    25141 caataaacaa tccaagcaat gatgaacttg ctccgactgt tgcagaaata aatatcatag
    25201 ttttcaagct atttgcatat aaatatgctg tagctgctgg tgtaattaac attgcaacaa
    25261 ttaatatagt tcccacactt tgcatagctg ttactgctac taaagttaat agaaccatca
    25321 atagataatg ataaaaactg acattcatcc ctattgcttt tgccattaat ggatcaaaag
    25381 aagtgattaa taattcttta aaaaatagaa ctactataac caacactaaa accgaaacgc
    25441 caattgtaat ccacttatca atatcttgta cagctaaaat atttccaaat aaaatatgaa
    25501 ataaatccgt tgagctattt gcaactccta taaggataac tcctagtgct aaaaaagaac
    25561 tgaaagtaat accaattgct gtatctcctt ttattacact attgcttttt ataaaagtaa
    25621 taatgataga agctaataga ccaaatacaa tcgcaccaat aaaaaaatta atacctaaaa
    25681 tataagaaat tgctactcct ggcaaaacag catgagaaat agcatcaccc ataagagaca
    25741 ttcctcttaa aataataaaa caacctactg ttccagaaac aatccctata actacagatg
    25801 taattagagc attctgtaaa aagtgaaagt catacagacc attaataaaa ttttgaagca
    25861 tattattccc cctctccaat aaatatagta tctccatagg ctttttttaa attttcttca
    25921 ttaaatacag attctacact tccgtgagca attagatttc gattcaaaag aataatatta
    25981 tcaaaatact tctttacctt gcttaaatca tgatgcacaa ttagcactgt ttttccctga
    26041 tgtttcaatt tttttaacgt atccataatt attcgttcac taactaagtc tatccctaca
    26101 aatggttcat ctaaaaatat gaaatccgca ttttgagcaa gacatcttgc taaaagtaca
    26161 cgttgaaatt gtcctccaga taattctcct atttgatgat tagaatattt tcccatatcg
    26221 accttttcca aagcctttga aactctttgc cattctgcat ttttaattct ttgaaaaact
    26281 ttcattccgg catatgttcc catagataca cattctttta cttttattgg aaaagtaaaa
    26341 tcaatatcag ctttttgctc tacatatgca atcttcacca gttttttatt gatatcttca
    26401 ttattaatgg agacctctcc ctgatgaggg ataatattta acattgcttt aagcaatgtg
    26461 gattttccag caccatttgg accaataata cctgttaaag caccttcttg tatttttaaa
    26521 gaaatctcgt tcaatgctaa aaaatcgtta taacaaacat taagcttttt cacttctaac
    26581 ataactttct ccttctaaat gataatgatt atcacgatca tatggtatac aaaaattata
    26641 ttttagtcaa ccctaaattt tatagtttag atttttttct gttgctgaag attagaatga
    26701 caaaactatt tgaaactacg cgtatagtaa gtagctttca aaatatataa aaaaggagcg
    26761 ttctcaaatg agaacatgct cctaaaacta ctttattggc gtatggaaaa attatacttg
    26821 aagtggatag cgggttgaat aagttgataa gcatcaaaac atttataaaa attattcaag
    26881 actatgtctg ttccatatac aaatatgaaa atcagtaaac tccatttaac ttttttcgta
    26941 gtgtgtcttt ttatacatgt ttatcgttat taatagtgaa taggaaaaaa actatttttg
    27001 atcattgaca attaaataca atttttgaat aacgatccct gctattcgag ctagaaactc
    27061 aagtacgcca tgacctgatt agcgacaaat acttagagat ttctttttgt tctgggaaaa
    27121 caaaatgcca tgacaatagc agcaaataat gatacttccg taaggctcgc cgtaaactgg
    27181 ggagaggtga aattcctatg tgcttaccca aagcaattca aaaatttcat aactactatt
    27241 ttaggagatg attttataga atccagagtc tacctgaata aaaaacaaac ctgtgcattt
    27301 ctaggaatcg ataataatac tctagataag tggattcgta tatataattt cccagtaata
    27361 agagttggtg gtgtagttcg ctttgacgta gaagatatta aagactttat gaatacattt
    27421 aaaataactt gaaattttct tctcgccgca tatactaaaa ttagtgaaat atactgcctt
    27481 acttttttta aggagtgagg attttgccta caaagctttc aaatgggaaa tataaaacta
    27541 atctgagata tccaaaaagg tttaaagaaa taacaggaat tgcatcagaa aagtatcaaa
    27601 aaacatttcc aaataggcaa ttggcgatta aagccgaaaa tgatatgaag aagaaaatag
    27661 aaaaagtttt gagagaggag aatgcaaact cattagaatt aaaaggaaaa ataacgttta
    27721 aaaaatttta cgaatctaaa tggcttccta gatatgaatt aggtcaaaca attagatcaa
    27781 acaggcctcc tagtgatata accattagta atacaaaaga tatatttcgg ttgcatattt
    27841 tacctatgtt tggtgaatac gcaatgaatt atctaaatat caatactgag ataatatcag
    27901 atgaattaac caaaaaatcg aaagaatatg caaatataaa aatcataaaa ggatatgttc
    27961 gttccatgtt cgatattgca gaaatactaa attatatcga atttaatcga actactaaaa
    28021 ttattcaaag tatcactgca cctaaaaaaa atgctttaga agaaaaaaga atacaagaag
    28081 gtaagcaagc actatcatca aaagaactta ctaattggat agaagcagta aatgatgatt
    28141 ttaataatca tttacttact tttcatgatt acacactttt tatgattacc ctgtatcttg
    28201 gtgatagaaa aagtgagact tacgctttgc aatggaaata tattgatttt gaaaaacaaa
    28261 cggtccgtct taaacatact ttagataaat atcaaagaaa aaagtttaca aaaggtcgaa
    28321 aagataccgt cattcaagta ccagaagttg taatgacatt gctaagtgaa tggaaatcgg
    28381 tacaagctga tcaattgttg aaattaaaaa ttaagcaaac gttagatcag tatctcttta
    28441 cttatactaa accatctgga gaagtaaatt gtcctgttca tgccgattac ctaaactatc
    28501 gaatcaattc aattaaaaga aggcatcctg acttagttca tttatctcca cacaaactcc
    28561 gtcatactta tgcaactata gctagacaag gtggagcaga tatgaatcaa atttcaaatg
    28621 ctttaacaca ctcagatatt tctacaacaa agatatatgt gaacacacca gatatagttg
    28681 ataaggctgt ttttgaagct tttcagagag gattgaataa gtgtgattga tatgtgttct
    28741 taaatgtgtt cttaaaaaca aaagaagcac tttccaaata attgaaaagt gccagaaacg
    28801 ttgatatata aaggatatta acgttttgag aattgtgaag ctttacgtgc tttcttaaga
    28861 cctggttttt tacgttcgac catacgtgcg tcacgagtaa gtaagccagc acgtttaaga
    28921 gctaaacgga agtcaggatc aacttctagc aatgcacgag cgataccatg acggatagct
    28981 cctgattgtc cagcgtatcc tccgccgttt acgttaacaa atacgtcata tgcgcctttt
    29041 gtttcagtaa cagcgaaagg ttgattgatt acttcacgca agtcagcatg tggaatgtat
    29101 tcttcaacat cttttttatt tacagtaatt ttgccagttc ctggtactag acgtacgcga
    29161 gcaactgcgt ttttacgacg gcctgtgccg atatattgaa cttgtgccaa tgaaattccc
    29221 tcctattaga ttaagtttgt gatgtctaaa acttctggtt gttgtgctgc atgtggatgt
    29281 tctgctccac cgtatacgtt caattttgta aattgtttgc ggcctaaagt gtttttagga
    29341 agcatacctt ttacagaagt ttcgatcaaa cgacgagaat ttttagcacg caattcgcca
    29401 gcagtgattg atttcaatcc tcctgggtag ttgctgtgac ggtaataaat tttgtcagtc
    29461 gcttttttac ctgttaattt cactttgtct gcattgatga cgatgacgaa atcgccagta
    29521 tccaaatgag gtgtgaaagt tggtttattt ttaccacgta gtacagatgc aacaactgtt
    29581 gaaagacgtc ccaatggaac atcagttgcg tctactacat accatttacg ttctacttcg
    29641 cctggtttgg ccatatatgt tgtacgcacg ttattttcct ccaatttaag ttctgatgtt
    29701 tgacatacac ttcgagtttc cggggctctt tgtggggcaa acaataccat ttaacatatt
    29761 acccgctaat ctagtcaatg tcaacgtttt tctgcttgat tgaaaaattt atatgaaact
    29821 ttacttcttt tctttctttt atataaaagg gtcgtttcct gtcaaaatga aaaacaaaaa
    29881 gaggctgtga caaaagtctt gtcacaaccc cttattttga ataaacggtg ttctcctgcc
    29941 aactcctcgg tcacaagtca caatatccat aaaacataag aagctgtttt ttaaaattgt
    30001 ttcttgtgac tcggagctga acggcaggct tacaacctct tggtaaacgg tgttcaatca
    30061 gctgaccctc ggtattagct caaaaaacgg caaaatatga ggaactattt tgacgtttgt
    30121 ttctctaata ctcaggtcaa aacgctgatt tcacaacctc tttttcggtc aagttcaaca
    30181 acagttttta cgcgtcgatc tctggcgctt tgcccaaatg agcttggatc atccagatac
    30241 gtttttctgt ggctgttttg aattcgataa agatatcttg tgttggatcg tcgccttctt
    30301 ctccagaaac ttcgattcct ttttggtaaa gatctgctag ataacgatag cctgcaacaa
    30361 gtgtttccaa acgttctgga atagtacgat cccagcggcc aatttcatca gggatttttg
    30421 tgttatctgc aaattcacgc aatgtagaat aaggtgaacc gtctaatgtg atcaagcgtt
    30481 cggatacttc atctaactga gcgttcaaat catccatgaa ctcatccatc attgggtgca
    30541 atgtaaggaa acctggtcct ctcatgtacc aatgtgtttg gtgaacaaca acagacattt
    30601 ggcttagatc tgcaactaat tgatttaata cttctttggt ttgttggaat ttcatctttc
    30661 ttgcctccta acattttctt acacttaaaa taatagtaga ctttcaatga aaagtacaac
    30721 aaaatcattt acctgagaac gattttcagt tcgctttttt atccgtaaag ccaatcattt
    30781 ttcagaatca gctaattctt ctcgcagttg agcaaattgc tgataagtct gtgctaaatg
    30841 cttttgtgtc gaaacaaata atggatagat tttctgatat tttttatgat cagtttcttg
    30901 tactccaact tgctctttca attctatcaa ttcctgtgct tcactgatac tttggatctt
    30961 tccgatgctt ctccagccta aaatcacagc tcctaatgcg gatgcttctg tgacagtagg
    31021 gaaaacaagc gggtaaccca gtacatcagc catcatctga cgaaaaatcg gagattgagc
    31081 aaacccgcct gtagcacgga tctctgtgag ctgattttcc tggcaaactc cttttaaaat
    31141 ccgataaagg ttgagacaga tcccttctag aatcgccttc atcatttctt tttctgtatg
    31201 ttgccgctgt aaaccgataa aactgccaaa cgcatctgcg tcccaaagcg gtgctcgctc
    31261 accaagtaaa tagggatgaa aaatcagacc attggcaccc gctggcacaa aagcgatagc
    31321 cgaaagcagt gcatcatatg gattttctcc tttttcttta gcagattgaa cgacttcctg
    31381 taaaaatgta tgccctgccc aatcaaagat gccagcacca ttgctagtcg ctcctcccac
    31441 tacccaatgt ttctgatcca gaacataaca aaacgtctcc atttctggat ggaactgagg
    31501 ctgagtagta ataaaacgaa gtgctccgct cgttcctacg gtcaaagcgg cgatcgtatc
    31561 accaaaagca cctaacccta gattagaaag cggcccgtct gcccctccca aaacaaatga
    31621 tatatcattt ggcaaaccta gttctgctgc tgtttttgat ggtagacctg ttacttgata
    31681 ggtggaagga aagacttgcg gcaattgctc tattcgaatg ttcaagtagt caagcacttc
    31741 ttctgcccat tggaattcat ggatatcaaa atatccagtt cctgaagctg ttccgtagtc
    31801 gctgatcagt tcgccaaaca atctataaaa gatatagtct ttgataccga caaaataagc
    31861 agtttttttg aataattcag gttggctgtt ttgcagccac ttgattttgt tcaatggtgt
    31921 cattggatga atcggcgtcc cagtctgccg ataaaattct ttgccaagca cggtatattt
    31981 cagtttttct gcttcgtccg ctgctcggtt atctgcccaa gtgatcattc gagataacgg
    32041 tgttttctct tcatccaaca atatcaggcc atgcatcgca gaggagaagg agacaaattg
    32101 aatgattgtg tctgaaggca actgtttaat tacttctttt ataacggtgc agacaccttg
    32161 aaagatcaat tcctgatctt gttctgccat cccttgtgtt tcttggatca aagggtagcg
    32221 aaaataagcg gatgctgctt cttgggcttc atctgtgaaa gcaactgctt tggtttgtgt
    32281 ggttccgata tctaatccga ttgaaacgtt cattttttca cctcaaattt tatgtatgta
    32341 caaaaattta gtttaagctt gcgtatcttg gttttgaaaa gggggagatg gcgtgttact
    32401 ttattttatc attggttgct gtatcggatc atttttatgt ttaaccgccc agcggattcc
    32461 gttaggtcac tctattattt atcctagatc tcattgtgtc aaatgctgtc gttcgctttc
    32521 ttggtatgaa ttgattccga ttctctcgat catcgtccaa cgatttcgct gccgctattg
    32581 caggtgtcgg ttgcccttct actatttgtt ggcagaagcc ttatgtggtg gtctttttgc
    32641 ctggttcttt actttttctt ccgcaagaaa tttttcgacg ttcatatgga tgctttcggc
    32701 tttgctattt tcgttgatgg atttatttta cttcatggtc cattctcaca cgctattctg
    32761 ttcttgggca attttatgga ttttttggtt gcaggccggc gtcttccaat ggcaaagtgt
    32821 gctgttaacg tttatcgtag gcggtgtgtg ccttcgctat ggtcaatcct atctcggagc
    32881 tggcgatact ctgttgatgc tttcttggag tggtggcttg tctctagaag agttgcttca
    32941 gatcttgttt cttgctagtc tgtttggaat atccgttttt tctgtttact ttatctgtca
    33001 taaaaaaaag ttggaaaagc tgcctttcat tccttttttg agcagtgcgt tgctgattgt
    33061 ccttcatctt aggtattgaa aaaaggttgt gacaaacgac ttgtcacaac ctctgatata
    33121 tagtgttctc ttaccaaatc cttgtgactt ggagctaggc gacaggttca caacctctcc
    33181 actaaatggt gttcaatcag ctgaccctca gtattagctc aaaatgctgg ttttacaacc
    33241 tctgatatac ggtgttccat aagtagcttt ttcggcttct tatttcttga cgccccaatg
    33301 tcgacgaagg aaacctgcac gtccagaacc ttctcgataa agattgtaat ggagcttgtt
    33361 tttcttgtaa taatcctgat ggtaatcttc tgcaggataa aattcgcttc ttggttcgat
    33421 ttttgtcacg attggcttat cgaatcgccc gctgtcttgc aatgcttgtt ttgaacgctc
    33481 tgcaatattt cgttcttctt catcattata gtagatcact ggacgataag aatcaccgcg
    33541 atcagcgaac tgtcctaatg cgtcagtcgg atcggtttgc tgccaataga tttccaccaa
    33601 ctgatcataa gaaataactt ctggatcata ttcgatctct actgcttctg tatgccctgt
    33661 tgtgcctgtt gttacttgtt cataagttgg atttggtaca tgcccgccag tataaccgga
    33721 tgtcacagaa atgatccctg gctgctcttc aaatggtttg accatacacc aaaagcatcc
    33781 gcctgcgaaa actgcttttt ttgtcatata aaatccccct tttttctcat cataccttga
    33841 tttccaaaag gaggataacg ttttgcttgt gacagaaaaa acggaaacta ttatatacgt
    33901 gatacggctt ctgataaatg ccaaaacgaa cgtacttgat caaaaacggt taaatcagga
    33961 atcacatatt cagcagaagg aagatttatg ttttcaccta ttcccaatac atgcatgtta
    34021 ccagccaaag ctgcttgaca accagcttct gaatcttcaa tgaccaagca tgcatttggc
    34081 ggtacattca attgctgtgc tcctttgaga aaaacttctg gatcaggttt tgcttttgat
    34141 acttgcgtgc cgtcaataag gacatcaaaa taacttgtca aattcgtttt ttctaaaatg
    34201 aggcgtgcat ttttgcttgc tgatcccaat ccgatcttga tgtttgtttt tttcaagtac
    34261 tcaagaacag ctattgcacc aggcaaaacg gatgtttcat ccattttttg aatcgcttga
    34321 agatatagat tgtttttttg ctcagcaaag gctgatttct ctttttctga gaatttctgt
    34381 tccatttttc caaatgacag caatcgctct agtgactcgg ttctggatat tcctttcagt
    34441 tgctcgtttt ctttctcagt gaatggaatg gacagctggt tagctaaaac cagccacgct
    34501 tcgtaatgat aacgagctgt gtctacaaga acgccatcta aatcgaatag tgctgctttg
    34561 attttcatcg attacctcct tgaaaatgaa cgacttgaag taaagatgcg atgtttttag
    34621 ctgtgcgatg aagattatca gtggcttgct gtaaagcttc ttgtaaaggc acagctgcag
    34681 gaaggatcgg aaaaacggcc gaaatgccaa catctgctgc tttttcgctt cctttaccaa
    34741 cacttccgca aatagctatc acaggaacgc cctctggtac tcttctagca atcccaactg
    34801 gcgcttttcc ttttagactt tgtccatcta gacgtccttc gcctacgatt acgaggtctg
    34861 cttgctgtac ccgtttatcg aactccagta aatccaatac gtagtcgatg ccagagacta
    34921 ttttccctga agcaaatgct aacagtcctg cacccattcc accaccagca ctactgcccg
    34981 atgcctcgat tacttgaggg gcaaatctca tataaaaatc atgcatggct tggtcagcca
    35041 ctgctaattc attttctttc aagccttttt gtggtccgaa aacaaaagaa gcgccttctt
    35101 ttccgcataa aagattagta acatcagtaa tgacagtgat tttggcttta atcagctcaa
    35161 taggcacgtt cgttttatca actgtagcaa ttttacctag attttctccc agtgcttcta
    35221 ctttttgatt atcttgatcg taaaaagcat aacctagacc ttctgccatg ccgatgccgc
    35281 catcgttggt actacttccg ccaacgccga tgatgatgtg tcgaacacct tgttgcaaca
    35341 attgtcgtat cagttctcct actcctcgcg tggataacct aagtggctgt tgttggttta
    35401 gtgggacatc tttcaatccg acgatttctg ccatttcaaa aatcgcagtt tgaccgtctg
    35461 aagcgtactg gatttctttg tttttgccaa atgcattagt aacgactgct gtttgccgct
    35521 tcaaccttaa actgtctgct aatgatgaaa ccgttccttc tcctccatcg cctataggta
    35581 gcagatcata ttcagcctga acaaaaactt cttgaaaacc tgattgaatg gcttgtgctg
    35641 cctctaaggc tgtcagactt tctttgaaag aatctggcgc aatcagaata cgcattattt
    35701 tcccctccgt tccaagatca gttctttttc cagctggtat tggtctccat tgacaaaaat
    35761 aggtaatgtt tctttcgtct ctttttttat tactatttgt ttttttgtca aaacaaatag
    35821 caacctgtga tttttccaaa taaaaggaaa agaaattttt tcccatgctg aaggaagtct
    35881 tggagaaata gaaagagttc cattcgttac tgtaatacct gaaaaaccaa atataactgc
    35941 tagccatagt gctcctaatg aagcagcatg aattccgtca tctgatgaat gcggatctgg
    36001 tcctaaatca atcaggcacg cttcttggaa taaatgatac gcccattcca tttcttggat
    36061 acggcaagcc tctatggcgt gaatcgcttt actcaacgat gagtcgtgga ttgttttgtt
    36121 ttcatagtaa tgcaaattct tagaaacgat tggttgagaa aaatactcag gtaataagta
    36181 taacagcatg acaagatctg cttgcttgct gacttggagt tcatttactt cgctacgggc
    36241 ataatcaagt aaaatagact ggctgccagg acgttctttg taagtggtca aatcaataac
    36301 tggcttactg agatacgtgt catcttgcgg aatcagctgt tctttgttag gcactggcaa
    36361 atataactta tctaaaaaat tttgacattc tttcaaaaaa agcggatcgc cagcttgata
    36421 tttaacagct aaggccacat tataatgcgc cagatagttc gtatatgcat tattgtcgat
    36481 atattctgtg tattcatctg gaccaatgac tctgtgtatc tctaaatgat catcgacttt
    36541 tacagctctg ctcagccaaa aagctgctgt ttcatgaatg agcgtttttc cctttgtttc
    36601 taaaaacgat tgatcctctg ttgcttggac atactcttga acggcataag caaggtctgc
    36661 actgatatga tgttctgcgg cagctgaagc aacttcttgt cttgttccgg ttttgatatt
    36721 caatgcagca aatttgggtg tctcttcctg accagtgtgt gcgctttccc atgggaaaag
    36781 tgcaccttga tagtgataat ctcttgcttt ttgccgtgcg ccttctaatc gatcataacg
    36841 gtacagcagc aaattctttg ctacttcagg aaactgatat aaaaagaaag gcaggataaa
    36901 aatttccgta tcccagaaaa catgcccttt ataaccttcg ccagttagtc ccttcgcccc
    36961 tacactaaca cttggatcat tgcggggagt cataatctgt aaatggtaag cagcgaagtc
    37021 catcgctaac tgatcgaaag aagaatcgcc ttggatcgtt atccgatgct gatgccaaaa
    37081 atctgtccaa taagacatcg actgctctaa aatttctgta taactaacgc ttttctgggc
    37141 ttgggcaata cttttcttaa acgggtctgt ttgctgaaga gaggtagaga gcacgatgat
    37201 cttctccagc gtaaaggacg tattttttct gatcttttct gaaaaggaag cggtgatccg
    37261 gcgatttttt gcttggaata cacctgcttt attgaattta gctgcaatcc caatatcaat
    37321 tttactttct gttgtctggt aggatgcact gatcaaatcg tcaccaaata ctcgcagttc
    37381 tttttcaacc aactgctgtg taccaaaatt ggtttgctgt ccatcgatac ccgttttcac
    37441 atctacttgg cagtcttcgg tcaacggctt gatgaccact ttcagggctg cctgatggag
    37501 attattttga tcagcaacac gacaagtaat gacttccact ttttttcctt gagcagtttc
    37561 aatgagcgca tagcgtttaa gctctcccat ttctaaatct agtgcccgct cgtatgcaag
    37621 aactttcatc gtctcaagag aaaaatgttc accgtcgatt tttatttccc atctgatgac
    37681 atctgggaga ttcactaact cggctgattc tgctcctaaa ggtcggttat atacaccagc
    37741 tgcaaacatt ccccttactt gattccgata gtcttcctcc tggcttcctc tgataccaaa
    37801 aaatccattt ccaatggtca tctgactcgc atacttattg atatactcta aatcaaactg
    37861 tttttcttct actttcataa ggtaataact tctcctgttt ctgccgaacg atataaattt
    37921 tccaccagat tctggatgac atatccttgc cagccatctg caatcatctg agtctggtca
    37981 ccgagcgccc gattcacaaa atgagccata cttagttcat gtgggtcatt aactggcgtg
    38041 gtcttttgta aggaaaccag ctttccatct acatcttcga atagttcgcc tggatacaga
    38101 ttagcacctg cctgatcgcc acaaaaagcc acattcagtt tgtcttctct gatattcaaa
    38161 gcaaacgaag tattcagttg gatgatcttg ccgttttcta attccagtgt gccaaaaatt
    38221 gcatcttcta ctgtgaattt gcttggatcc catttcccga atttccctga attttttttc
    38281 gtaccaattt tttggaagct gtacgcccga acgcttttta ctttaggaaa tccagtaata
    38341 tacatcgctg tatcaagcat gtgaatgcca taatcgatca aaggcccgcc tccttgaagt
    38401 tctttgtcga taaacgttcc ccagcctgga acaccagacc gacgaagcgc attcgcttct
    38461 acgtaataaa tatccccgag aacatcaaga tgattcttga tcattgttgc ttcgtcagaa
    38521 aaacgatgct ggaatccata agccaaaatc tgcccgtttt tatcggctgt ctctgccatc
    38581 tttcttgctt gatcagctgt catagctggt ggtttctcgc acatcactga agcattagct
    38641 tcgagggcag ccaatacatg ttcgaagtga aagcgattgg gtgtacagac actcacgata
    38701 tcgggagaaa tctcattcag catttgtgta actgatgtat acgcatgaga aaatcctgta
    38761 cgtcggcaaa agtcttctgc tcgctggctg actggatcaa ccacagcggc caattctaag
    38821 acatctgatc ttttttgata ataagcagca tgtacttttt ctgctacttg tccagcgcct
    38881 atgattgcta ctttcttcat ttatttctct ctcctttgct tatttactta ttgttattct
    38941 ttctctattc tttctctaaa tcatttaaat gacgcgctgt ttccagacag tttttcaagt
    39001 attgcaatga ttcctgataa attttcactt cattttctcc tcgcaaacga cattcatata
    39061 ccatataacc ttcatactca ttttgaagta gcgtttgaaa atggcgagta aaatcaattg
    39121 cgccagatcc tggttggtag cgatgattat ctgctaaatg gatatgaccc acatactctt
    39181 tttgcgtagt caatgctttt gaaatatcat cttcttcaat attcatatga taaaaatcag
    39241 cgatgatctg cacatgtttc aattgattag agtcaatgta atgacgcgca tccgctaaca
    39301 aattgatcat atggtcttgg tatcggttca aaggctccaa ataaataacc gtacctgttt
    39361 cttcggctac tttatctaaa taacgaagtg attcgctgac tgctttatag tctccttctt
    39421 tgcttcgggg tgaaaccatc ggcggtaatc gataagtgaa cattccccaa gctgctggga
    39481 taacgattcc tttgcctcca acctcgctta gtgcacgcaa aatctcagca atttgacgta
    39541 caccattaag acgccgttct tcgataaagt caccgatcca cccatcgtag ccgttacaag
    39601 ctgtcactac tgggatccct gtttctttga ttgcctgttt gactgtgtcg atattttcta
    39661 ttaacaactt accgtcgatt tcgtattctt caaatccaca gtctttgata aatctaaatt
    39721 tttctaaaat gtctcttggg aaaaatgctt gatcttgcgt accgaatttc attgttctcc
    39781 tcctcggact tgaacgccca ttttgatact ttcttctggg tgctggtcta cataacgcat
    39841 gtaagcttct gcgctttctg caaatggcac aactggatca atgatttctt cacaatccaa
    39901 gtaaccgttc atcaataatt cccaacatgt gtcctcgatt cgtttacggt tccatcttgg
    39961 gtaatctggg tttggttcac tagatgctcg agcaaagacg atttttgcat tattgaaatg
    40021 tgcttcgcga ccaagattaa acccagctgg aaaaggttta gcaaaggcaa cataagaaat
    40081 gatcccgcca tagccgatcc ctattaaaga agcttgtaat gcggcagcac tgccactcgt
    40141 ttcaataact gaatctactc cacgtttatc tgtcaattcc tttagacgaa aaccgacatc
    40201 tgctttcata ggatctagcg tgtaatcggc accgtgtttt tctgctaatt ctctacgctg
    40261 cgctatggga tcgatcccaa cgatggtagt agcacctgct tttttagcta attgaatcgc
    40321 aatttgcccg atagcgccta aaccgaagat tgctacagca tcacctgctc gaacatgtgc
    40381 ttcccggata ccacctaaag cgaattgagc aggatcgtaa catactgcat ttttccattg
    40441 atccaaccga tcaagtttac gtagtttata attatcaaca ccgtttgtaa tcagcgtttc
    40501 acgaatgggt ccataagtgc agactaactc tcctttttga tactctgtca ccaaactgcc
    40561 cgtttcttcg atcgtaccga caatcatatt tcctaattga aattcgccaa ataccacacc
    40621 gcgttggctt ccttcttctc ttggcaagaa taggcgccat tcttctgaaa attcttcatc
    40681 gataaatgga cttaatcctc taaagtcgac aatttctgtt cctggttttg gagtagcgaa
    40741 aattactcgg atacgtactt cgttcttttc gatctcacgg tcttcgtaat ctttgaatcc
    40801 ggctattctt ggttctaatg caactaattt tttcatcttt tctttcctct ttcttttctt
    40861 tttatccttt tacgccgcct tcgactaaat cacctttaat aaatcgttca gataacgcat
    40921 acataatgat gactggtaaa gcggtcacca atgaagcggc catcattcgt ccccaaatgt
    40981 agtctggtgt actgaataat gtgttcaaac cgattggtaa agtaaatttt tcggaggtcg
    41041 acaggaaaat cgaagcaaat aaataatcat tccacgaaac cataaagcaa taaacgaaga
    41101 cagaaatgat tccagaaata gccaatggta taatgatccg aataatgatc tggatccggc
    41161 taagaccatc gatcattgct gcttcttcaa tatcagtcgg aatcgtgtca aaataacttt
    41221 ttagcatgaa tatcgcagta ggaagtgttt gaacgaccat cgtgataatc agtgctgtat
    41281 tggtatcata caaacctaga ccggaaatga ttttaaataa tggaacaacg atcaaaatac
    41341 cggaaaacat ataaacggtg taaaagctag cattgatttt cgagcgagct ttgaagcgta
    41401 gttttgacaa agcgtatgcc ccgctgatcc caatcactac tgcagttacg gaggctatca
    41461 aggagatcac taaactattt ttgaaataca tgatgaatgg aaaaatctca gcattgaaaa
    41521 tatcctgata atgttcaaaa gtccattctt tcggaaaaat agtttgtgtc gttgcaattg
    41581 cttcttgtga atccttgaat gatgtcatca gcatgatcaa aaatggaaac aaattgacag
    41641 ctaatagcaa caaaatcgtc gcgtaaaata agattttcct gctcttttta tttttcattt
    41701 caagtccacc ttctttcgtg agagcaagac gaccgctgca ataatgatga acaatagaat
    41761 cgatatagct gctgcttttc caagatcatt gaacgcaaag gctgtctgat acaaataaac
    41821 gcctaagata ttgactttat tcgtcagtag atagacatct gtaaacatat agaacatcca
    41881 gatagatcgt aaaacaatca cagttgctag tactggtttg attgccggca gtgtcacgat
    41941 cttgaacttc tgccaagcat ttgcaccgtc catttcagct gcttcataaa gcgttttgtc
    42001 gactgtttgc aaaatagcca aaaatgaaat aaacgcatac ggatagtatc tccagatagc
    42061 aaacaaaacg accataaaga agctagcaac tggctggtcg aaccacagcg gtgcttcgtt
    42121 gaaaagctgc aacctatcta ctaacagatc attgacgatt ccataactat tattgaacat
    42181 atatttccaa gcaaaaatca aagaaataga tggcgtaaca taacttaata aaatcagtga
    42241 acgcaccgtt ttcctaaatc tgaacttgcg gttaagcaaa atagctacgg ctaatcctaa
    42301 agctgtactg cctgctacaa ctaagaatgt gtaagcaact gttaaaccta aagaacgata
    42361 aaattcacta tcacttaaaa tttcaagata attgcttaat ccgataaaaa cggcgtctga
    42421 gttaggattc agcggcatat cgaaaaaact gatctgaata ttggaaagca ttggaatcag
    42481 cactaaaaca gctaataaaa gcatgcttgg tgctaaaagt cccatggcta attttaagtc
    42541 agaatgtttc cttggacgat tcattgaaac ctccccctga taatctgcca atagctatct
    42601 acttttttcg aacaccattg gcagaacttc ttgttatttc gtattttttt gtgcgtcagc
    42661 taaagcttga tcgacatctt cgtttccgac agttacttgg ttcactgctt ttccgatcac
    42721 acctgagctt gtgatatctc ccattttcgt gaaattcttt tccccgacta gtccaaagac
    42781 ttgtacttca tcaaaagcac tagcaatctc agatggcagc tcgccaaaag acttgatgac
    42841 ttcattttct ttgtaaccat ccagttcgac tacttgttta ttcactggtt gagcaccacc
    42901 aggagacatc aagacccact tttccatgtt tttcggttga gataagaaag ctgtgaatgc
    42961 ttcagcagcc tctttttgtt tctcgtctag accagaagaa atcgtcaatc cagatactgt
    43021 gccatagaca gcttgatctt tttgtgttgg gatagcaaaa ccgatatttt ctgagttccc
    43081 cgcttcataa acagaaggca aaatgtaagt tgaatagatt gccattggtg ctgttccatt
    43141 catgaaagca tctttgattt ctgtcacatc attagagcct ggcatcgtgt attgggataa
    43201 gttttgataa aacgacaatg cttctctcat cttttccgta tcgatcgtta cctcgccttc
    43261 atcatccaaa acatttgctc catttgacaa agcaaattga gagaacgctt gctcactcat
    43321 tgttccttca gcagtaggca tagcaattcc atattgttta ttctctttat ctgtgaaatg
    43381 ttgagcaatt tttaaaatat catcccaatt ttctggttct gaaaaaccag cttctgataa
    43441 tttttctttg tcataccaaa taccttgaac ccagccgcta attggtgcag caatatagct
    43501 ttttccatct tctgaacgaa caaggttttt ggctccgtca taataattat cttctccagc
    43561 ttcctcgata acgttttgga ctgccgtctg atcaatcagt tcatctttat ccatcacttt
    43621 agcaaaatcc tgactgactt ccattacttc tggtaattta ccggaacgag ctaacgtaac
    43681 gatttttgta ttgaacgctg attcttctac cggcacttgc ttgattttga tgtctggatt
    43741 ttctttttca aaatcagcaa ccagttcatt gatcacactt aatcgttcct gttcgacaga
    43801 agagtgcatg aattgaatcg ttgtctgttg tgattcgtta gagctttgac tttcaccaca
    43861 agcagtaaga agaaatccag tagctagcag acaggtagta acaataattt ttttcatttt
    43921 tttccacctc ttattttatt attttcagcc acatatattc atacggctgg aatatcaact
    43981 ttcgttcttg actgcaatgg ataatttgtc cagaaaacag gtctaggtaa tctccttttg
    44041 gtaaaagata acctgctgat tgattggaaa aattatggat aactaagact ttctcttcct
    44101 tcgcttcgcg aataaaactg ataaaagaat ctgctgagtc cagtaagtaa atcaaactat
    44161 tcggatggaa ggctttttct ttttttcgaa tagatagcat ctgggtcaat tcaccatata
    44221 ctgctgttct taatggatct gtctgcaact ctttctcaat atctgctaac aaaaattttt
    44281 gccgattgat ttttcgattc tctcctgttt tttctacgcc tttgtaatca tttcgacttc
    44341 ccagcacact ttgaatataa attgccggca cgcctggtaa cgttaccaaa aatgaatgag
    44401 cagctaaaaa gcgtttgctt ctccattccg tcgtatctgt tggagaactt aacgcatcta
    44461 gatacgtagc gtttatctca tatggactgg tcgttccatc tgggtttttc ttgtagttta
    44521 ctcgggcacc ctctttcttc agctgttcca ctaattcaag gatcgttttt tccggcagaa
    44581 tacctcgtac cggattcaat ccgataccgt catgggaggc taaaaaatta aaaaaagttg
    44641 tttttttcga tgcgaaatcg atggttttta accattcttt caacttactt gtattacccg
    44701 aacgaatcgc ttgaagggtc aaaggtggta gcgaaaactg atagaccatc tgtgcttcat
    44761 tctcgccatt cccaaaataa ctgatattat ctttgtgtgg cacatttgtc tcagtgatca
    44821 ataccgttcc tggagctgct tcatcagcga ttgcacggaa taattggatg attttatgcg
    44881 tttctggcag gtgtatacaa gaagttccta gttctttcca taaaaatccc acagcgtcca
    44941 agcgaataaa tgaagcgcct ttttctaagt aaaacaaaag aatatcgatc attttcaata
    45001 aaacttctgg atttttaaaa ttcaagtcta cttgatcttc gctaaaagtc gtccatacgt
    45061 gtttttcttt cccgtttttg tcaatgaacg ttgtaagtaa cgggctggtc cttggtctag
    45121 tcacgctgct tgtatctgta tccagtggca aatcaataaa aaaatctttg tacgtatcat
    45181 tcaagtttaa atattcctga aaccaagtac tttcggcaga catatgattg cagacaaagt
    45241 cgaacattaa ccgtgtttcg cttctcattt cttctatatc ttcccatgtg cccaaacggt
    45301 cgttgattgc ctgataattt acaacggaga aaccatcatc agatgtatat gggaaaaatg
    45361 gcaagaaatg tacaactcta aatgtatctg ccaaatactt ttgataaaat ttcttaaatg
    45421 tacgcaaagt tggttgctgc tcttcaaaaa attgatcccc ataagtaatc aagaaaatat
    45481 cttcttcgtc ccaatgcagt ttgcctgcct ggcctttctt ctgctgataa acactgactt
    45541 tttgttcaat catagaggcg gttttctcag acaaatctga gtcgcgataa atcgcttgta
    45601 aaagttttgt atacatcgga ttcccctttc ttttttggga gcgttcccaa cgatttgatc
    45661 atattatgga aaacgcttac tgtcaatagc atttttttaa ataattttga tatacacaaa
    45721 aatacaaata agaaagctta tgtatcaata attaatagct tcttaaaaaa atatggtctc
    45781 tcatgatcat gcaagctatt cctaaatttt caaaatcata gacaaaaaag aatgtctatt
    45841 gtactatgga attattctaa aacgctccca aaaaggaggt gcatataaaa ttgaaaccta
    45901 ctatttatga cattgcgaaa aaagctaatg tctcaaaatc aactgtctcc agagtattaa
    45961 ataaccagcc aaatatctct gataaagcaa aagcaagtgt acttcaagca atcaaagaat
    46021 tgaattatga accgagcaaa attgcaagag gactgtcagg aaatggtttt gacgcgattc
    46081 tagcattatc tcatcgtact tctcatacga cctatggcaa tccttttttt tcagatgtta
    46141 ttcaatccat ttctgccgtt tctgaacaaa ataacttcga cttgattcta caaacggcaa
    46201 aaacaccaga agaagagcta gaaaaatgcg cgaaaaagat ccaagaaaaa atgatccgag
    46261 gaattattat tttaagctct acgatggatg aaacacttct ttttgaattg gatcaatata
    46321 atattcctat tgtggtaatc ggaaaagtcg gaaacgacta cacgaacatc tactcagtgg
    46381 acacggataa tttccaggat agttacgatt tggtggaaac actgatcaag aatggacacc
    46441 gtcatatcgg ctgtctccat gctccgacag aagtttatgt atccaatgat cgtctcaatg
    46501 gttaccgtca atgcttattt gatcaccacc tagatatccg taatgaatgg attatagacg
    46561 gcggctttcg cttagaagat agcatggcag caatgaatga tttaatgaag ctacgagaaa
    46621 gaccaactgc gattttttct accgatgcat taaaatcatt gagtatttac aaagtggcag
    46681 aacaatctgg aaagcagata cctgatgatc tttcgatcgc aggattcagt gatcccttat
    46741 tgtcctccct attttcgcca cagttgactc ggatagacat tccgacaaat cagttaggaa
    46801 tcaaagccac agaactttta ttccaactaa tccaccacca ataccctgaa gaaaaaagaa
    46861 tcattctttc aaccgagatt actctcacag attcgatcca acaacaagaa atgtagaaca
    46921 gaattataaa agaacagaaa cactgaggat aagtcagtta ctctgttctt ttttcaatca
    46981 ctcatccctc gttaccccct cccggtattt tagcgaattt ttcttatttt ccatctgttt
    47041 tagatagctg tggataacta taatgtttgt gttaaactat tttttccaca gggaagaaaa
    47101 aactattttt aaccacgttg tgtataagtt tttatagttc gtttttttaa acacatttac
    47161 ccttatttca atccatgtta tttggggata cttatctcta aaacataaat agaataacag
    47221 acatgtctac tggttttgtt ttattcgagg aattattcaa taatagctag caaaatatta
    47281 aaaataaccg ctctatcttc ccatcaattt attttgcatt caaaaaatga tctatcttta
    47341 cttttgttga ttgacttctc ttggaagatg atcgttccga taccttaatt ggatacgaac
    47401 cgatgttgtt aagatccttt cccacttttc ttctagttag acaaattatt ttagacaaac
    47461 caaaaaagag gttgtgacaa taatttgtca caacctcttt tttaaaatat ctattttgtt
    47521 tgattgttca ttgcgtcttc tgcagccatt tgcgcttcaa tatctgaaat actgtagaca
    47581 ttttcgcttg ctacaccgac ttcttttggt tccatgtcac gataacgttg cataccagta
    47641 ccagctggga tgatcttacc tataataaca ttttctttca aaccaagaag aggatctttc
    47701 ttaccgcgga tcgcagcatc cgtcaagaca cgagttgttt cttggaatga tgcagcagat
    47761 aagaaactat ttgtttctaa tgatgctttt gtgataccta gtaagacagg tcgagaagtt
    47821 gcaggaacgc ctcctgagac tagcgtattg tagttttgat ctttaaagtc tgcgatgtct
    47881 aataatgtac ctggtaagat atctgtatca cctggatcca ttacacgaac tttacgcagc
    47941 atttgacgaa ccattacttc gatatgcttg tcgccgattt ctaccccttg catacggtaa
    48001 acacgttgta cttcacgaag caagtagttt tcaacagata atacatcgcg aacttgcaac
    48061 aattgttttg gatcgattga tccttctgtc aatggtgcac cacggtggat catatcgcct
    48121 tctgctactt tcatacgtgc tgtataagga acagtatacg tacgtgtatc tgtttttcct
    48181 ttgattgtta cttctttggt acgtgttgca ggatcttctg agatatcgat gacatcccct
    48241 gttacttctg tgatcactgc ttgccctttc ggattacgtg cttcaaagat ttcttggaca
    48301 cgaggaagac cttgagtgat atcgtctccg gcaaccccac ctgtatggaa tgtacgcatc
    48361 gtcaactgag taccaggttc accgattgat tgagcggcga tcgtaccaac tgcttctccg
    48421 acttcaacgt cagagccagt tgcaaggttg cgtccgtaac aatgtttgca gacaccatgt
    48481 tttgtattac atgtgaagac agaacggatt gtcactgttt cgacacctgc atcaacgatt
    48541 tgttttgcga tgtcttcgga aatcaattgg tttcctgcaa tgatcaattc atttgtttca
    48601 ggatgacgta cttctttacg ggtgtaacgt cccactaaac gatcttctaa tgattcgatg
    48661 atttcatttc cctcgcggat ggcttggatt tcaagacctc ggtcagtacc gcaatcttct
    48721 tcacggatga ttacatcttg ggccacgtca accagacgac gagtcaagta acctgaatcg
    48781 gcagtcttca aggctgtatc ggtcatccct ttacgagccc cgtgagttga gataaacatt
    48841 tccaagacgg atagtccttc acggaagttc gaaatgattg gcaattccat gattcgacca
    48901 ttaggagcgg ccatcaaacc acgcattccg gcaagctgtg taaagttgga aatgttacca
    48961 cgagctccag aatcagacat catgaagatt ggatttttcg cttccaaacc ttccatcagt
    49021 ttttgttgga tgctgtcttt tgcagcattc caaacagcaa tgacacgttc ataacgttcg
    49081 tcatctgtga tcagaccacg acggaattgt tttgtaatag tttcaacttg tttatgggca
    49141 ttttcaatga tttcttgttt ttcattcagt acactgatgt cagcgatccc tactgtgata
    49201 ccggcatgtg tcgaatgttt gtaccctaag tctttcatac ggtcaagcat cttagaagtt
    49261 tctgttactt tgaatcgttt gaagacttct gcgatgatgt ttcctaagtt tttcttcttg
    49321 aacggtaata ctaattcttg ctctttgata tgtgcaggaa tatcagtacc agcttcaaca
    49381 aagtatttgt ccggtgtttg gacggtcaag ttgaagtctg ttggttcatt cagataaggg
    49441 aattcaggag gcatgatctc gttgaagatg atcttaccaa ctgttgtgat catcgtacgt
    49501 tctttttgcc attcagtaaa tggtttttca cctaatgagt tagggttcac accgatacgt
    49561 gaatgtaaat gaacatatcc gttgcgccat gcgataacgg cttcgttttg atctctgaag
    49621 accattcctt cgccttcgcg tccttcttct tccattgtca agtagtagtt acctagaacc
    49681 atatcttgag aaggagtaac aactggttta ccatctttag ggttcaagat gttttgagca
    49741 gctaacatca acatacgtgc ttctgcttgt gcttcttcat tcaatggaac gtggacagcc
    49801 atttggtctc catcgaaatc ggcattataa gcttcacata caagtggatg caaacggatt
    49861 gcgcgtcctt gtactaaaac tggttcgaat gcttggatac caagtcggtg aagcgtaggt
    49921 gcccggttca gcaatactgg gtgttccttg attacgtctt ctaaaacatc ccatacttcg
    49981 tcttcttgac gttcgatttt acgtttcgca tttttgatgt ttgaagctag ttcgcgttga
    50041 acaagctcac gcatcacgaa tggtttgaat aattcaatgg ccatttcttt tggaagtcca
    50101 cattggtaca tcttcaagaa aggtccaaca acgataacgg aacgacctga gtagtcgaca
    50161 cgtttaccta gcaagttttg acggaaacga ccttgtttcc ctttcaacat atgagaaaga
    50221 gatttcaatg ggcggttacc tggtccagta actggacgtc cgcgacgacc gttatcgatc
    50281 aatgcatcta ctgcttcttg cagcatacgt ttttcatttt gaacgatgat tccaggtgca
    50341 ttcaagtcta acaaacgttt caaacggtta ttacggttga ttacacgacg gtaaagatca
    50401 ttcaagtcac ttgttgcaaa acgtccacct tctaattgaa ccattgggcg taaatctggt
    50461 ggaataacag ggataacgtc cataaccatc cagcttggtt gatttcctga agcgcggaat
    50521 gcttctaaaa tatccaaacg gcggattgca cgtgtacgtt tttgtccttg tgcagatttc
    50581 aattcttctt tcaattcggc tgcttctgca tctagatcaa cattatcaag taattgttta
    50641 atcgcttctg cacccatagc agcttggaat tcttgtccat attgttcacg tttttcgcga
    50701 tattcgcgtt ctgtcaacaa ttgttttttc tctaaattcg tatttcctgg ttcgatgact
    50761 acatatgaag caaaataaat gatttcttcc aaagcgcgtg ggctcatgtc taatacaaga
    50821 cccatacgtg aaggaatccc tttaaagtac cagatatgtg aaacaggagc tgctaattca
    50881 atatgtccca tgcgttcacg gcgaactttc gaacgagtaa cttccactcc gcatcgatca
    50941 caaacgattc ctttataacg gatacgtttg tattttccgc aggcacattc ccagtctttt
    51001 gtaggaccaa aaatccgctc acagaacaac ccttcacgtt caggttttaa ggtacgatag
    51061 ttaatcgtct caggtttttt tacttcaccg taagaccagc ttctgatttt ttcgggagaa
    51121 gccaaaccta tttgcatact ttcgaattta tttacatcga tcaaaagggg ttccctccat
    51181 ttcatttaat ggcaaaccgc aagacggctg atcttcgacc gccttgcagc tcatagaccg
    51241 gattagtcta aattatcttc tgcggtttca aagttttgga tgacatcgtc tttttcatct
    51301 tccacttgtt cagcagcttt cttttctaat tctttggcag tttgctgttc ggcaaatttt
    51361 gtcaaagcat caacagtgat caaatcgtcg tcttcatcgt ccatgtcgcg aagttcgatc
    51421 tctgtttctt cgatatccaa gacacgcatg tccagaccta gtgattgcaa ttctttcacc
    51481 aatacgcgga atgattccgg tacacctggt tttggaattg gttcaccctt gacgattgct
    51541 tcgtatgttt tcacacgtcc gactacgtca tctgatttgt atgtcaagat ttcttgtaaa
    51601 gtataagccg caccgtaagc ttccagtgcc catacttcca tttcaccaaa acgttgtcca
    51661 ccaaattgag ctttacctcc caatggttgt tgtgtaacca atgagtaagg tccgattgaa
    51721 cgtgcatgca atttgtcatc gaccatgtga gcaagtttga tcatgtacat cacaccgaca
    51781 gacacacgtc catcaaatgg ttcacctgta cgtccgtcgt acagaacagt tttcgcatcg
    51841 ctggccatac cggcttcacg aactgtttcc catacgtctt catcacttgc accatcaaat
    51901 actggcgttg ctacgtggat acctaactga cgtgcagcca tacccaagtg taattccaat
    51961 acttgcccga tgttcatacg tgaaggcacc cctaaaggat tcagcatgat atcgattggt
    52021 gttccatcag gtaggaatgg catatcttct tccggcataa tacgggatac aaccccttta
    52081 tttccgtgac gaccggccat tttatcccct tcatggattt tacgtttttg aacgatataa
    52141 acacgaacta acatgtttac gcctggtgac aattcatctc cagcttcacg agtaaagatt
    52201 ttcacatcgt gaacgatacc gccgccgcca tgtggtacac gtaatgatgt atcgcggact
    52261 tcacgagctt tttcgccaaa gatcgcatgc aatagacgtt cttctgcaga taattctgtt
    52321 accccttttg gcgtgacttt ccctacaagc aaatcgccat cttggacttc tgcaccaata
    52381 cggatgatac ccatttcgtc taaatctttt aatgcgtctt ccccgacatt agggatttcg
    52441 cgtgtgattt cttcaggtcc taattttgta tcgcgggctt ctgattcata ttcttcaata
    52501 tggatagaag tgtatacgtc atcttttacc aaacgacggc tcatgatgat cgcatcttca
    52561 tagttgtacc cttcccaagt catgaaacca actaaaacgt tttggcctag agccatttcg
    52621 ccttgttcca tagatggtcc gtctgccaga gtatcccctt tttcgacttt ctcacccaaa
    52681 tgaacgattg gacgttggtt gtagcttgtt ccggagtttg aacgacggaa ttttgttaca
    52741 tcgtatttat ctaatgcacc gttgtcgcgg cgtacgcgga tttcagaagc atcaacgtat
    52801 tcaacgacac cgtcatgttt acataacaaa gcagctcctg agtcatgcgc tgatttgtat
    52861 tccataccag taccaaccca tggtgcttga ggattgatca acggcactgc ttgacgctgc
    52921 atgttggcac ccatcaaggc acggttggag tcatcgtttt ccaagaaagg aatacatgct
    52981 gtcgcaacgg caactacttg tttaggtgat acgtccatgt agtctacttt atcaatagat
    53041 acttctaagt tttcactttg agcacgagcc ataacaacgt cttgagcaaa tgttccatct
    53101 tcattcaatg ggctgtttgc ttgggctacg atgtaatggt cttcgatatc agctgtcaag
    53161 tagtcgattt ggtcagtaac acgacctgtt tcacggtcta cacgacgata aggtgtttcg
    53221 ataaatccga atttatttac tttcgcataa ctggacaaac tattgatcaa cccgatgttt
    53281 gggccttcag gagtttcgat cggacacata cggccatagt gggagtagtg aacgtcacga
    53341 acttcataac cggcgcggtc acgagtcaat ccgccaggtc ctaaggctga aagacgacgt
    53401 ttgtgtgtca attcgccaag agggtttgtt tgatccatga actgtgacaa ctgagaagaa
    53461 ccaaagaatt ctttgatact tgccacaact ggacggatat tgatcaattg ttgaggtgtc
    53521 aatgtttcag tatcttggat cgacattctt tcacgaacca cacgttccat acgtgctagc
    53581 ccgatacgga attggttttg taaaagttca ccgactgaac ggatacgacg atttcctaag
    53641 tggtcgatat catcgacatt gccgatacct tccatcaagt tcaagaaata gctcattgaa
    53701 gcaatgatgt cggctggacg aaccgttttt acagctgctt ctggatagcc gtttccgatc
    53761 acgtttactt cacgttcagg gtctcttggt gaaaatactt tgatgacttg gacagtcatt
    53821 ggatctgtga ccacaccgtc ttctgatgga tagtaagtca ctgaatttag gccattatca
    53881 atgaaaggag ctagtgtttc catgacttgg tgagtcagaa ctgttccttt ttcaacgatg
    53941 atctcgcctg tttctggatc aactaatgtt tcagctaatg tcagattcaa tagacgtgtt
    54001 ttcaaatcta attttttgtt tactttgtaa cgaccaacgt ttgctaagtc gtaacgtttt
    54061 ggatcgaaga aacgagcatt cagcaagtta cgtgagctgt ctgctgtttt tggttcgcct
    54121 ggacgcaaac gttcgtaaac gtctttcaaa ccttcttctg ttcttgagtc gcttgcgttt
    54181 ttatgcaagt ctttttcgac tgtattgcgt aatgtttcgc tgtcgccgaa gatttcaaag
    54241 atcgtatcat cggaaccaaa tcctaacgca cgaactaata cggttaaagg aatcttacgt
    54301 gtacggtcga tacgtacata agagatgtct tttgcatcag tttccatttc tagccaagct
    54361 ccacggttag ggataacagt agaaccgaat ccttcttttc cgtttttgtc gacttttcca
    54421 tggaaataga cacctggtga acggactaac tgagaaacga ttactcgctc tgccccgttg
    54481 atgatgaatg ttccttgttc tgtcattaat gggaaatcac cgaagaatac ttcttgtgct
    54541 ttgatttcgc cagtctcgcg gtttgtcaaa cgcaatgtta catgtaatgg tgcagagtag
    54601 tttgcatcat gcgcacgagc ttctgctact gtatactttg gttctttcaa ttcataatct
    54661 acaaattcca aagatagatt gccattgaag tcatcgattg gcaaaatgtc ttcaaacatt
    54721 tctctaagtc cctcatctaa gaaccattgg taagagtctg tttgaatttc gatcaaattt
    54781 ggtaattcca acacttcact gattcttgcg aaacttctac gttcgcgatg ttttccgtat
    54841 tttactacgt gtccagccaa gctcttcacc cctctaaaat gtcattcaaa aagactaccc
    54901 gatcaattgt tacaataata tttcaaatat tatttttagg taacaacaga cattttttgc
    54961 ggcttttagg caaaaaaaaa ccaaaaatag aacattctct tgaaacctta cttcgtgagc
    55021 ttctactttt gtttccttat attaaagccg aaacggacaa tatttcgttt ctgccttttc
    55081 ttcatcagat agtttttgca acttcatata ctacacttat tttctataaa agtcaacact
    55141 tcctggcgtt tcttctgctt tttcgcaaca ttttcttaat gttttcaatt ttatgttttc
    55201 aattatctga caattcttaa ttgacagacc tttttatctc ggttatactt tagaaaattc
    55261 attttgtgga ggaacttcta tgcgaaccaa catcaaccaa atcgcaccaa cacaactgaa
    55321 taacttttac tttagctact ttagataggt ttgtattcat catgatggat acaaacgtta
    55381 aacgacagtt tgtatctatg atggctaaag aactttgtat tgcagcaaaa agccacgtag
    55441 acaaaatgct aagtggctga gcagtgaaaa tacaaagtaa aagtatcaag cattctactt
    55501 tttcaattta ttttccgact tttcaatcca tttggacatt ttgtctgaat ggattttttg
    55561 tttcccaaaa ttaaggagga aaaagaaatg aaaaaaaata taatcatcgg attaggcgct
    55621 gccctgctac tagctttagg aggttgcgca caaaaagaca attccgcaga ggcatcagat
    55681 ggacaaaaaa caattggcgt attgcaactt gtacaacata actcgttaga tgctgcctat
    55741 aaaggattca aagaaggatt agcagaagaa gggtataaag aaggcgataa tctaacaatc
    55801 gattatcaaa atgcccaaaa caatcaagac aacctgaaaa gtatgagtga gaaactcgtc
    55861 aaagaagatc ctgatttgct acttggaatc gccactccag cagcacaatc tttattaaat
    55921 gaaacaacca ctatcccgat gactgtcaca gcggtcacag acttggaaca agcccattta
    55981 gtcacatcca atgaaaaacc tggcggcaat gtgactggca cgacagatat ggtgccaatc
    56041 gaaaaacaaa tcggcttact gttaaatatc gtgccagacg ccaaaacaat cggcatcatg
    56101 tataacaacg gtgaagcaaa ctctaagatc caagctgatt tggcagaaaa agaattgaaa
    56161 aaagcgggcg tcaaagtcaa agtactgaca gcaaattcca caaatgatgt ccaacaagtg
    56221 acgaaaagtc tagctaaaga tgtagatgga atctatattc ctactgataa cacgttcgca
    56281 agtgcggcaa gtgttgtcgg tgaagtcgca aaagaaacaa aaacacctgt cgttgctggc
    56341 gcagttgatc aagtcaaaga cggcggtcta gcaacagtag gaatcgacta cgaaagcttg
    56401 ggcaaacaaa cagggatcat ggcagcaaaa atcttagacg gcaagaatcc aagtgaactt
    56461 cctgtcgaaa cagcgaatga tttggaactg tatgtcaata aagacatggc caaagcatta
    56521 ggcatcaatc cagacagcat caaagaagct gaataaaaca caggaggatt ctcatgacaa
    56581 atttgctctc tcttttctta tcatctgcat cgcaaggggt cttatgggcc ctacttgcga
    56641 ttggtgtata cgtcactttc cgtatcttgg acattgccga cttgactgct gaaggaagct
    56701 ttcctttagg tgcagggatc gcggcagtaa gtattacaaa tggctatcat ccattgacag
    56761 cttgtcttct aggcttttta gggggtgcgg cagcagggct tgtttccgga ctgcttcaca
    56821 caaaattgaa aattcccgcc cttcttgcag ggatcatcac catgactggt ttatattctg
    56881 tcacatcccg tgtgatgggt gcgccgaaca tctctttatt gggtcagcca acgatctttc
    56941 attgggtcga gtcattcggc ataacaaaag acaacgctgt actgatcgtc ggtctgcttt
    57001 ttgctttgat cgttgtatgt ttacttgttt tgttcttacg tacagaaaca ggacttgcta
    57061 ttcgtgcaac tggcgataat ctggcaatga gcgaagcaaa cgggatcaac acagataata
    57121 tgaagatcat cggttatatg atttctaatg gatgcattgc tctttctggc tccttacttg
    57181 cgcaaaacaa cggctttgct gatttaaatt ctggtattgg aacgatcgta attggacttg
    57241 cttctattat catcgcagaa gttttgttac gtaaccagcc actatttctc cgtctcttga
    57301 ctgtcgtttt aggtgcggtc atttatcgtt ttattttagc gttagtcttc gaactgaatg
    57361 ttgaaccaag tgattcgaaa cttgcttctg ctttcgtgct agtgatctgt ctttctttcc
    57421 cgcagatcca gcaaaaattc catttacctc aaaaaaagaa aggcggtagt gtcaaatgaa
    57481 tgacgtctta acattggaaa atctccatca tacctttgaa aaaggaacga tcaacgaaaa
    57541 tcatgtcctg aaaggcatta atctttcctt agatcccggc gacttcgtga cgatcatcgg
    57601 tggaaatggg gctggtaaat ctactttgct gaacagcatc gccggtacat tttctgtcga
    57661 tcagggtcaa attttattga atggaaaaaa tatcacgaaa aaatctgttg tcgaacgttc
    57721 gaaatccatc agtcgcgtat tccaagatcc taaactaggg acagccgttc gtttaacggt
    57781 agaagaaaat cttgctcttg ctatgaaacg aggaaaaaaa cgcggcttct tccgtggtgt
    57841 caaaccacaa gaccgaacat ttttcaaaga ccagctggtt cggctaaatt tggggttgga
    57901 aaatcgttta tcctcagaaa tcggtttgct gtctggagga caacgtcaag ctattacgtt
    57961 attgatggcc acattacagc gtccagaact gatcttatta gatgaacaca ctgcagcgct
    58021 tgaccctgct acttccaaaa cggtgatgga attgacagaa aagctgatca ctgagcagaa
    58081 tctcactgct tttatggtca ctcatgacat ggaagacgcg atccgctatg ggagtcgact
    58141 gatcatgctg catcaaggaa ggatcgtcgt agatatccag caagaggaaa aacagcagct
    58201 cacagtgccc caattattag cgttgttcaa acaaaacagc ggcacggcat taaaagacga
    58261 tcaagtactt ttatcttcat aatcagaaag aggatgggac aaaaaatgaa cgcccaccct
    58321 cttctttttt ctctttcctt ctttcgtttt tgctttatgt atcaaccgtt gtttcttcga
    58381 aaactatttt gttctctcct tcttcctgat agaatggatg ggaaaggagc ttgatgccat
    58441 gacaacgaaa gacaaggtac ttcaattatt gaaaaagaca ccagattttc tttccggaga
    58501 aaagttggca cagcaactgg aagtttcccg gacaagtatt tggaaagcca tcaaagaatt
    58561 ggaaaaggca ggctatcgtt ttgagcacgg gcctacaggg tatcgttacc tgccctctga
    58621 cgtactagat gcaaaagaaa tccagcaaga gctacggtcg tttattcctg atttgaaagt
    58681 acaaacgatg ttgacttctg aatctacgat gaaagatgcc aaactggcag cagctcaagg
    58741 agattctgtt tcgaagttat ttgttgctga tacacaagaa caagctcgtg gacgttttgg
    58801 acggcctttt ttcgctgaac ctggacgagg aatctacatg agtctcttgc tccatccaga
    58861 caaacggttt gatgaattgc ctcaatatac gatcatcgca gccagtgcct gtgtgaaagc
    58921 cattgaatct gtaaccggtc gttctgcttc gatcaaatgg gtcaatgata tctacatgga
    58981 tggaaaaaaa gtctgtggga ttttatctga agcagtcagt gatatggaaa gcggcaggat
    59041 cagtcatatc atcattggga tcgggcttaa tttttctgtt cctcaaaatc attttccagc
    59101 tgatctccaa gaaaaagcca tctctgtctt tcccgacgga caagctagtg tcacaaggaa
    59161 tcagctaatc agcaaaatct ggcaagaatt gttcacttat ctttctcacc cagaaaaagc
    59221 gttggacttc tataaagaaa aatcattcgt attgggaaaa accgtgtcgt tcaatcaaaa
    59281 aggaacgact tatgtcggtc ttgcaaaaga aatcggtgat catggcgaac tgatcgtcca
    59341 aacaaatgaa cagaagatga gtttgtcttc tggcgaaatc agtctcacat ctatccgtac
    59401 gcaataatta tcagatttca caaaaggaca cctgatctca tacagtgtcc ttttttctga
    59461 attctcttca aaagactctc cttattctat cagctgacag tttctctatc ctctagaaaa
    59521 aggtattcta aaaatatcta taaagcatgg tacactttct ttaaatgaat caatgcaaaa
    59581 aacaagagag tcatcagttt acacgatcaa aagagatagc aggccgcttc ctttttcaat
    59641 cttatagata gaccatgggt aacgataaaa acaaaaaaga acttgcgact tattttgccg
    59701 taagttcttt tttagctagt gccttaatct cttaatccca acaaacgtct cgcatagatc
    59761 acctgaccag agtgcatagc agcatcatca ataatagaaa cgagacgcat tcctcgtgtg
    59821 actggtggtg tccagttctc gtcaattact tcatctagct cggaaggttc cagccttctt
    59881 aagtaagaaa tagcaaaatc agcagctcct cgcaagtatc ctagcagatc ttctatttga
    59941 ttagcttgga tctgctttgc ttgttccaga ctatgcttcc aatcttgttc gttatctgct
    60001 atcgtcatcg gaaaccggct ttcccagtct tgactatgcc aaattggaga agttcctgct
    60061 aaatcagcaa tctggaaatc taattctcga gcggtatgcc aagccagcca agtcattgat
    60121 ttgatctgcg gggctctatc ttccactgga aaacgattgg cctcctcaat agagacgcct
    60181 tcaaacaaac gttcaaaccg atcgtatgcc cgatccacac tctcgcatag catatctaaa
    60241 taatccattt attttcctcc tttttattta tcctacttac tttttcctaa ttctaggata
    60301 cctacttata tgttactaca cacccttatt ttttacactt taacagtcac catcatcaat
    60361 agtttggaga ttgaaagaaa aagatagtgt cgaactatca attgaacaag aagcaacaat
    60421 atgaacgttt cattaatatt ctttaaataa acaatgattt ttcaaacgaa cagtttaaga
    60481 ctaggagaaa aagagtataa taggaaaatg tttgaaagga aaggaagggg aaaaatgaaa
    60541 aaaagggcaa gtttactagt cctagttgct ttgttgttca atatcttttc gtatggtgtg
    60601 gccgtttcag cggaaacata cattcctaaa gaccctggga atatttataa tgatctaact
    60661 ggtaactatg gcttgttagg aattgctagt caattccacg tatttgctaa aaataagaca
    60721 actgtgaatg cacatacaga cggaaatata gcaacacatg aacttgatgc aaaaaataat
    60781 tttggtacag agcttcatga aggtttattg aaccaagaaa taaattatat acaaaaaact
    60841 gattctttcg ctaattcttc aggcatacct acaggtagca caagaatgaa taaatttgtc
    60901 gttggcagta acgtggatgt agcacttcaa gatggtcatc ctacagtcaa tggatccagg
    60961 cttgatcact taactccaga tgatttttat aaagatcgtg ctgggcatga atatattgat
    61021 ttcgagcagg aatttgctaa attgaatgta gcagcaaatg atcttgcaac gatcactcca
    61081 gctaaaacat atactgctgc agattttcca gacatgaaca atcggacaat cgatttaact
    61141 gatttatcag ataccgggtt tttattggta aatatcgatg cagaagtgtt aacgatgaat
    61201 acgccacttc aaatcattaa tccaaatgat caagtagttg tatttaatgt catcaacagt
    61261 tcttctgcat tgaacgtaca gtcaccaatc aaatacaatg atcgttcaaa tcatgaaaca
    61321 gaagattttt cggatgcgaa tatctcttgg aattttggaa atgagatgac tgatttgaca
    61381 atcagtgctc cttttcaagg aacaatcctt gcgcctaatg ctactatccg cattactcaa
    61441 aaccaagacg gaacgattat aggaacaaat gtgattttag atgcagctac caatcgttgg
    61501 gatcctaacg aaatatttat cagcgacaat ggtacagata cgacagacac aagcgacagt
    61561 acagatacat ctaccacaag tgatagctca gattcatcaa cttcaagcga cagtacagat
    61621 actacctcta ccacaagtga tagcacggat acatcagctt caagcgacag tacagatact
    61681 acctctacca caagtgatag cacggacaca tcagcttcaa gtgacagtac agatactacc
    61741 tctaccacaa gtgacagcac ggacacgtca gcttcaagcg acagtacaga tactacctct
    61801 accacaagtg atagcacgga cacatcagct tcaagtgaca gtacagatac tacctctacc
    61861 acaagtgata gcacggacac gtcagcttca agcgacagta cggatacatc taccacaaca
    61921 agtgatagca cggacacgtc agcttcaagt gacagtacag atactacctc taccacaagt
    61981 gatagctcag attcatctac cactacaagt gatagcacgg acacgtcagc ttcaagtgac
    62041 agtacagata catcctctac cacaagtgat agctcagatt catcaacttc aagcgatagt
    62101 acagacacgt cgtctaccac aagcgataca cgtcatgcaa gtgtctcctt ctcaaaaaca
    62161 agttcggata acaggacgaa ctttaactta actaataaaa caagcaatga caatggaaac
    62221 aaatcaaaaa gagcacttcc taaaactgga agtcaatcga ataattggat aacactcgca
    62281 ggcgttatat tacttgtaat cggtttaaga atcactttta tgagttataa gaaatcgaaa
    62341 aggtaaaaaa atattcaaga taaaaaagag caacgtcgct aacggacgtt gctctttatt
    62401 tttgcttttt atcataaata caagaactac aattcaattt gtcaaaaaac acgtagattt
    62461 ccttctgaga aatttattta cgtatcgaac ctttccctat tcagtctttg cgatccaaaa
    62521 tcagttgggt aagggaaaag agattctctt ttgttcgaag agaggtggca ggtaatttct
    62581 ttatttcctt caaagccttt tctgtgtaag aggccgctaa cttcctagct tcttccacgc
    62641 ctcctgtatg ttcaacgatt ttgcgtacgg cgtcgacatc ttcttctgtc attgccgctt
    62701 tcttctctaa ataaggtagt agctttggtt tgtttttttg caaagagtag atcaacggta
    62761 aagaatagac cccttgacgc atgtcttcta gtacaggttt gccgatttcc tggctctctt
    62821 ttgtataatc taaaatatca tcgataatct gaaaggccat cccgatagaa agtccgatat
    62881 ctcctgcttt ttttgcgaag cgctcgcttg ttccactttc aaaagcgcca acagaacaac
    62941 ttaattggaa cagttcagct gtcttacctg aaatattttc caaatattct tttacagaaa
    63001 gttcataatt atagcgatca tccatctgcc ctaattcgcc attcaatact ttttccatac
    63061 tgcgagaatt caattggaga cttttcaagg aagtggagta atcagacaat agtttaaaac
    63121 agcagacaaa taaatagtct cctgcataga cagaaacgac attaccaaac tgactacgga
    63181 tattaggctg accacgtcgc agatccgctt catcaacgat atcatcatgg atcagtgtgg
    63241 ccgtatggag catttcgata gaagctgcca aagcgatggc tttgtctcga tcttgctctg
    63301 gtccaaattg tgagaacaat agctgatagg caggacgcaa cagctttccg ccggcatgga
    63361 tcgtttcttt gatggcttgt tcgacaggtt tgtttttcag gcggacagtt tcttccatcc
    63421 gttttagtgt ttctgtcaat tctttactta aagcaggata gttttcccac attggatgta
    63481 gcttcataaa aaaactcctt gtgtgacgat tattttttgg taaacattgg gctccatttt
    63541 tgtttggttc cctgctcata atctttttgg aatcgttgaa gtgtctgcac atgtcgtaaa
    63601 agataattcg ctgcgatccc gattgcaatc cctgataaaa tccctaaaac tgaaaggact
    63661 ggcaagtaca acatgactgt ccatgattgt gcaatgaaag aagccatcaa aagctgtccg
    63721 acattatgca tgaaaccgcc ggccgcacta attccgatga tactgacacg ttttggtcct
    63781 aattgcttga ccaataacat tccaaaataa ctaagcaaag caccactcat actataaagg
    63841 aatgtggaaa tcgttccgcc tagcaaagta gtcaggaata gtcgtagcca aagtagtaag
    63901 aagctgtctt gttttttcat ggtaaaaatg gcaacgatcg tgattagatt ggctaagcct
    63961 agttttgcac caggcgcaaa agaaaaagga taagggatca tattttccaa taaaccaatg
    64021 atcactcctt gtgcaacaag tagcgaaata taaatcattt tttgtaattt actcatggtg
    64081 tgtgaaggtc taataaatca gactgtcttc gtcacctcca tctgttgaac ggacttcgat
    64141 cactaattta tgtggcaaac agacgatggt ttcgccattt ttcgttgccc agcctcgacg
    64201 tacgcagatc tggtcatcgc aatctgcttc ttttatccgt actcgatcgc catctatctc
    64261 gatcaagtta taatttccgt gttcatcttt gtaagtatat gtgttgctct cttctccagc
    64321 catcaaaggg aatgttttga tttctgtgcc atccacacgc agtaccgctt ctttttctgc
    64381 tgttgcggtg ttttgctgaa gaccaaaaat catgactggt aaaaaagaaa gaacagtcaa
    64441 aagcaaaata ataaaaacat cccaaggttt gagcagactt ttttcagcaa attctttaag
    64501 gctcattttt gttctccttt ttccagatta actcacccaa ttttagcata aaaacaagag
    64561 gttgtgacac ctgtgtcttg ctttgagtaa taagaacgaa gaatagaaaa cagctttcca
    64621 tgttttctaa ttctttgggc ttattaccga gaagcagcag gtggaacacc gtttataaca
    64681 agaggttgtg acagaagtgg gcaacttcaa gaaataagaa ggaatttacg aaaattactt
    64741 ttcaagtttt tgtgaaattt cggcttattt ttcgaagaag ctacttctgg aacaccgttt
    64801 ataacaagag gttgtaacag aggttgtgac acctgtgtct tgctcatgga ggcttatctc
    64861 gggcatctta ttcctttaat tttacataaa aaggaggctg tgacggtttg tctcagcccc
    64921 tttctattga aatcttgcat tcaaataacg ttctttatct atttatttct tgccgtactg
    64981 ggaacttggt ctaccgtacc accaattacc tagtgtacgt tgtagccatg gaattaccca
    65041 gcgatccaac ccaatcgcac gtccggaacc gttcatcaat gcgaaagcaa caaaaatgaa
    65101 ccaaatgttt acccagtaaa acatgcctga taagcagaac gtgatcacca atacaatcgt
    65161 tgtcgcactg catagccatg tgaataatcc agcaatcaat gctaatgcca aggcaatttc
    65221 aaagaatacc atgaattttt ggaagaacat tgctacatca ttgtttggca tgatcacttg
    65281 catgatcttt gtgaaccagt ctggtgactt ctcaatcaca gccattggtt cttcaccgta
    65341 tgcataactc aaaccaaaat gtgcagcttg agtcgctgct tctccggcac ttgcccctgc
    65401 tccgctggca gctgtggttg catcaccagc agcagaagca ccactggcaa catctgttac
    65461 attgtattcc catgtccaag ggaacacact gtttccagtg aaccatgatc cttcaccaaa
    65521 ccaagtactt ggtttgaaca cttcaccatt cccaatgatt tttttggaag cttcaacgaa
    65581 ccaaactaaa ccatagaaaa tacgaagtgg cacactccat aagacattgc cgtaacgaga
    65641 agaatgtcct cgagtcacgt tgcgatcatc tttgatatgg aaaaactcat gcattaaata
    65701 ttggaacata taataaccag aacgaatatc aaagaagtat ttcaaattca cgatatgttt
    65761 cattacgata gctaaaaatc cgcttaaatg gatcttatca aataggttag ctacccccca
    65821 tttggcacca atagaaacca taaatccttg atagtttcct ttaaatttat gcttttctcc
    65881 gcctttgatc gaagccacga tattcgctgc ggctgtgtgt cctgtttgtt cagcagcttg
    65941 aacgatctgc ggtgtcggtg ttcctgggaa ttcttcataa aagactaaat caccaatgat
    66001 atagatgttt ttatcttcgt agccttttgc ctgcatatat tcattggcga tcaaccgctg
    66061 tccgcgtgct gattctaatc caaagtcagc agcgtcagaa gttcctttga caccagctgt
    66121 ccaaatcagt gtgtgagtcg gaatgttgcg tccgtctttc aatttgatat gatcagcagc
    66181 cacttctacg attggtgcat tcaataataa ttccacgttg tgcttacgca aatagcgttc
    66241 agcttttgca gcatcatttc gatccaacat attcaagatc gtcggcattg cctctacgac
    66301 catcagtgtg atttcgtttg gatctaattt ataatctttg gctaggcgat ttttccagtc
    66361 gatcagttcc ccaatcattt caataccggt aaatccagaa ccacagacaa cgaaagtcag
    66421 catggcttta cgcagttcat catctggttc caatgcagct tttgctactg tcttctcaat
    66481 atgttcccga atccttacag aatcttcaaa tgaccaaagg gtgaatccgt gtgctttgac
    66541 accaggtgta ccaaagtcat tcggctctcc gcccattcca aggatcagct gatcaaaagg
    66601 ataggaacct aatttggtct tgacgatttt tcgttcttta tcgataccga ttacagtgtc
    66661 tgtgacaagt tgtacattct tttgacgagc aaacaaatgc tgcagatcgt attggattgc
    66721 ttctggttct acacgtccgc cagctacttc atgcaactcg gtcatcatag tatggtacga
    66781 atgacgatcg atcagagtaa ttgctacatc ttcttttttc agtttctttg ccaagtattt
    66841 tgtagctgaa acaccggcat atcctgctcc tacaacgaca atattttgct ttgccattga
    66901 tcatccgctc cttatagact atctatataa tgaatatatg ataattcttt tagtttgata
    66961 cttattacac aatctccata tattatatag atgttctgtt cttttgtcta actccttttg
    67021 gatttttttc aaattttaaa ataaagcgtt ttttcacgaa atttgctgaa agaaaacgtt
    67081 tgaaatatta catgggttat ttatcaaaaa tagttgactt ataggctaaa aagattagaa
    67141 tgtataatga atcacaaaga aaaattgtgg actagaaatg gaatggtggg gtattaaaat
    67201 gaagaaaaaa cgttttgtaa ctggatttgc cgtattagca ttttcagctc tagtgttagg
    67261 cgcttgcggc gcagatgaca acggaagctc aaactcttct tctgaatcat ctacagcaca
    67321 atcttcgact gcaaaaagca caacagaatc atctgcaaaa gttgtagctg gcggagattt
    67381 gcaagacggc acatataaat tagaagaaaa aaattattca aatggttacc gtgcagtctt
    67441 tgaaatggtt gtaaaagacg gcaaaatcac tgaatcaaaa tatgataacg tcaatgaaaa
    67501 cggcgaatca aaaacaaaag atgcagaata caacaaaaac atggaagcaa aatctggcac
    67561 aaacccagaa aaatacattc ctgagttgaa tgaccaattc ttaaaagcac aaagcgcaag
    67621 cggtgtggaa gtcgtaactg gagctactca ctcatctgaa agcttccaaa actacgcaca
    67681 acaattgatc caagctgctc aagctggtaa cacagataca atcgaaatcg acaatggtgc
    67741 tgatttgaaa gacggcactt acaagcttga agaaaagaac tactctaacg gctaccgtgt
    67801 gcaattcgaa atgacagttg ctggcggcaa agtaactgaa tcaaactttg attatatcga
    67861 caaagacggt aaatcaaaac aagatgatac agaatataat gaaaatatga aagcaaaaag
    67921 cggtacagaa ccaaaaactt acatccctac tctgaacgac gaattcgtca aagcaatggg
    67981 cgaagaagat ggctcacctg ctaatgtaga agttgttaca ggtgctacac acagctcaca
    68041 ttcattcatc atgtacgcac aacaattagt gaatgcagct gaaaaaggcg acactcaaac
    68101 aatcgaagta gataatatcg taacagaaaa ataatcacat aatcatacta ataaaagttg
    68161 gagggtggga cgatagccag attaggcagt cgtctcacct ttcaaaatga agagaggaaa
    68221 aagatgcgga aaaaactact attttcctta atcacactgg gcatctcttt tatggtgcta
    68281 actggctgca gcaactcagc aaataaaaca accactgaaa gttcagcagc taccaagata
    68341 ttgaaagatc catattctga tgaacagttc ttgttaggga cttacgttcg tatccgtgtg
    68401 tatgatgaag gcaaagaatc agcattgaaa cctgccttcg atcgagtaaa agaacttggt
    68461 gataaaatca cgatcaatca aaaaggttct gagatcgatg aagtgaatga gcaagctggg
    68521 atcaaaccag taaaagtttc tgatgacgtc tacacgttag tcaagcgggc ctacgaatat
    68581 agccaagatt ctcaaggcgg attcgatatg gcgattggtg caatcacaca attatggcga
    68641 atcggttttg atgacgccag aaaaccttcc caagaagaaa tcgatcaggc attgaaatta
    68701 gtggattatc ataagatcga actaaatgac aaagaaaaaa ctgtttatct aaaagaaaaa
    68761 ggaatgatca tcgatttagg tgcaatcgcc aaaggttaca ttacggacga agtagtgaaa
    68821 gtcttgaaaa aacaaggagt gaccactgcc atcgttgact tgggcggtaa tgtctatgtc
    68881 ttaggccaca gcccaagagg cgaaaaccaa gactggacag tcggcatcca agatccgaac
    68941 atggctcgcg gatctgtttt aggttctatt aaagaacgga acaaaacatt agttacctct
    69001 ggaatttacg agcgttattt agaagtagat ggcaaaaaat accatcattt gtttgatcca
    69061 aaaacgggtt atccgttcga caatgatatc gcaagtgtca cgatcataac agataaatca
    69121 attgatggtg acgggttgtc tacagcagtc ttttcaatgg gtgtgaaaaa aggactggaa
    69181 tacgtcgaaa gcgaattgaa taacgggacg gaagcgatct tcgtgacaaa agacgataaa
    69241 gtatatgtga ccgatccaat caaagacacc ttcaaattat cggaggactc ccattacacg
    69301 atgggtgacc gttctgaact aaaataagga gtacgaacca tgacactaaa aatcttttta
    69361 gaagtagttg aaatacgaac aaaggtggct agtgtatttc cttttgcggt aggggtatta
    69421 ttctctatcg ctttttttca tgagatacat tggctaaaca cttttttatt ttttattgga
    69481 atgctagttt ttgatttagc gactaccgct atcaataa
//
LOCUS       DAJSBL010000008        68432 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.008, whole genome shotgun sequence.
ACCESSION   DAJSBL010000008 DAJSBL010000000
VERSION     DAJSBL010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 68432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 68432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..68432
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.008"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            93..974
                     /locus_tag="ONT85_000639"
     CDS             93..974
                     /locus_tag="ONT85_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR2377635.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTVVEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            987..2597
                     /locus_tag="ONT85_000640"
     CDS             987..2597
                     /locus_tag="ONT85_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetronasin resistance protein"
                     /protein_id="HCR2377636.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRMGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(2674..3333)
                     /locus_tag="ONT85_000641"
     CDS             complement(2674..3333)
                     /locus_tag="ONT85_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HCR2377637.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(3616..4281)
                     /locus_tag="ONT85_000642"
     CDS             complement(3616..4281)
                     /locus_tag="ONT85_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HCR2377638.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(4428..5033)
                     /gene="dhaL"
                     /locus_tag="ONT85_000643"
     CDS             complement(4428..5033)
                     /gene="dhaL"
                     /locus_tag="ONT85_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HCR2377639.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(5061..6071)
                     /gene="dhaK"
                     /locus_tag="ONT85_000644"
     CDS             complement(5061..6071)
                     /gene="dhaK"
                     /locus_tag="ONT85_000644"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HCR2377640.1"
                     /translation="MPERKDGNKLKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRV
                     VAKNQKAEQVGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEAD
                     SGKGVLLIVKNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAG
                     TVLVHKIVGDAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDE
                     IEYGVGIHGEPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQ
                     FVFMNDVLALLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTI
                     SW"
     gene            complement(6046..6492)
                     /gene="dhaM"
                     /locus_tag="ONT85_000645"
     CDS             complement(6046..6492)
                     /gene="dhaM"
                     /locus_tag="ONT85_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HCR2377641.1"
                     /translation="MKAKPIVSNYVKERKILVKKSILLISHSQKVTDGIKEMIEQMQK
                     SEEVQIFSLGGINGEIGSDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEE
                     EQQQRYHLVDAPLVEGAFAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            6595..6915
                     /locus_tag="ONT85_000646"
     CDS             6595..6915
                     /locus_tag="ONT85_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HCR2377642.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(7027..7896)
                     /locus_tag="ONT85_000647"
     CDS             complement(7027..7896)
                     /locus_tag="ONT85_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HCR2377643.1"
                     /translation="MGKVMKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTI
                     SSSGAENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELE
                     EALEAAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQ
                     QAKEFNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAAS
                     FQRIKDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGV
                     LVAAAYGIIRKKK"
     gene            complement(7992..9295)
                     /locus_tag="ONT85_000648"
                     /pseudo
     CDS             complement(7992..9295)
                     /locus_tag="ONT85_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
     gene            complement(9292..9801)
                     /locus_tag="ONT85_000649"
     CDS             complement(9292..9801)
                     /locus_tag="ONT85_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2377644.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(9976..12111)
                     /locus_tag="ONT85_000650"
     CDS             complement(9976..12111)
                     /locus_tag="ONT85_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR2377645.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(12194..13849)
                     /locus_tag="ONT85_000651"
     CDS             complement(12194..13849)
                     /locus_tag="ONT85_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCR2377646.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(13851..14165)
                     /locus_tag="ONT85_000652"
     CDS             complement(13851..14165)
                     /locus_tag="ONT85_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HCR2377647.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            14335..14595
                     /locus_tag="ONT85_000653"
     CDS             14335..14595
                     /locus_tag="ONT85_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HCR2377648.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            14609..14923
                     /locus_tag="ONT85_000654"
     CDS             14609..14923
                     /locus_tag="ONT85_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HCR2377649.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(15653..16207)
                     /locus_tag="ONT85_000655"
     CDS             complement(15653..16207)
                     /locus_tag="ONT85_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HCR2377650.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIRLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(16333..18261)
                     /locus_tag="ONT85_000656"
     CDS             complement(16333..18261)
                     /locus_tag="ONT85_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HCR2377651.1"
                     /translation="MDNEMISQLQFDRIKKEIQARAIGNYSKKRISDMTVSTNLQTVL
                     DRQEETREARLILESSQHVPFMGLPRIDALTEQVKKGLILQPTDLIEYADFLRSSRMI
                     TKFFDKNQYQTPLLFAYSKHLPDLINVEELIDQKIKNNKVSDDASRNLRKVRKQLQLI
                     EKEIQSKLLKFLRHPKNKEMIQEAMIVQKGEYYTIPIKASYKNKIDGTIIDESNKRTT
                     VFIEPTVISKLNEHYQLLKAEEISEEYQVLAALTGAIAENEEVIDLLIETITVLDIIF
                     ARAKFSREINGSTPRINKSEHIIIKQGRHPFLPEHAVPLDVEIGKDYRGLIITGANAG
                     GKTVVLKTVGLLTLMAMFGMQVPAKEGTELAVFDEIFVDVGDHQNLENALSTFSGHMQ
                     NIAAILKKIKRNTLVLLDEIGSGTEPNEGAALAIAIMESMYEQGALIIATTHYGEIKK
                     FAGDHEDFVPAAMAFDREALRPKYQLRVGETGESQALWIAHKMDMSMKLIHQAERYLA
                     EKAYRTQKKEFLPKTAVTNRKENKKERQLFAKGDRIFVNEYQKEALVYEDIGEDTIDV
                     YLDKKIIHVPRQRVRLVRSAEDLYPTGYDLDSLFIDYKTRKRQRDLERGSKKAHKVLV
                     KEMRKRQEERRVNDENSK"
     gene            complement(18567..19283)
                     /locus_tag="ONT85_000657"
     CDS             complement(18567..19283)
                     /locus_tag="ONT85_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HCR2377652.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(19284..21107)
                     /gene="glpO"
                     /locus_tag="ONT85_000658"
     CDS             complement(19284..21107)
                     /gene="glpO"
                     /locus_tag="ONT85_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HCR2377653.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(21121..22617)
                     /gene="glpK"
                     /locus_tag="ONT85_000659"
     CDS             complement(21121..22617)
                     /gene="glpK"
                     /locus_tag="ONT85_000659"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HCR2377654.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(22753..24213)
                     /locus_tag="ONT85_000660"
     CDS             complement(22753..24213)
                     /locus_tag="ONT85_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR2377655.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(24203..25498)
                     /locus_tag="ONT85_000661"
     CDS             complement(24203..25498)
                     /locus_tag="ONT85_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCR2377656.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(25830..26507)
                     /locus_tag="ONT85_000662"
     CDS             complement(25830..26507)
                     /locus_tag="ONT85_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HCR2377657.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(26537..27628)
                     /locus_tag="ONT85_000663"
     CDS             complement(26537..27628)
                     /locus_tag="ONT85_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HCR2377658.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEEAYKELYLPSPLYNFLKARYQ"
     gene            complement(27729..28163)
                     /locus_tag="ONT85_000664"
     CDS             complement(27729..28163)
                     /locus_tag="ONT85_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCR2377659.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(28747..30204)
                     /gene="ascB"
                     /locus_tag="ONT85_000665"
     CDS             complement(28747..30204)
                     /gene="ascB"
                     /locus_tag="ONT85_000665"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HCR2377660.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(30201..32096)
                     /locus_tag="ONT85_000666"
     CDS             complement(30201..32096)
                     /locus_tag="ONT85_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCR2377661.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPITLLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSPVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(32154..33929)
                     /locus_tag="ONT85_000667"
     CDS             complement(32154..33929)
                     /locus_tag="ONT85_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HCR2377662.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARLPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(33979..35475)
                     /locus_tag="ONT85_000668"
     CDS             complement(33979..35475)
                     /locus_tag="ONT85_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HCR2377663.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(35669..37291)
                     /locus_tag="ONT85_000669"
     CDS             complement(35669..37291)
                     /locus_tag="ONT85_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000415199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HCR2377664.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESINAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTVPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(37740..39053)
                     /locus_tag="ONT85_000670"
     CDS             complement(37740..39053)
                     /locus_tag="ONT85_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HCR2377665.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGIAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(39298..39522)
                     /locus_tag="ONT85_000671"
     CDS             complement(39298..39522)
                     /locus_tag="ONT85_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2377666.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(39542..39964)
                     /locus_tag="ONT85_000672"
     CDS             complement(39542..39964)
                     /locus_tag="ONT85_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HCR2377667.1"
                     /translation="MESVYRQERKSNRKKGQMMIQKTRVMLYVNDVEMICRFFVEKIG
                     AEISETIELPEEFKSIVLSISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETI
                     YENMEEPGEITDNNGILTFNFSDPEGNYFVIGKAESSD"
     gene            complement(40108..40818)
                     /locus_tag="ONT85_000673"
     CDS             complement(40108..40818)
                     /locus_tag="ONT85_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2377668.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(40836..41333)
                     /locus_tag="ONT85_000674"
     CDS             complement(40836..41333)
                     /locus_tag="ONT85_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR2377669.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(41370..41822)
                     /locus_tag="ONT85_000675"
     CDS             complement(41370..41822)
                     /locus_tag="ONT85_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCR2377670.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(41957..42562)
                     /locus_tag="ONT85_000676"
     CDS             complement(41957..42562)
                     /locus_tag="ONT85_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HCR2377671.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(43012..43632)
                     /locus_tag="ONT85_000677"
     CDS             complement(43012..43632)
                     /locus_tag="ONT85_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR2377672.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLCQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(43634..44503)
                     /locus_tag="ONT85_000678"
     CDS             complement(43634..44503)
                     /locus_tag="ONT85_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HCR2377673.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(44526..44933)
                     /locus_tag="ONT85_000679"
     CDS             complement(44526..44933)
                     /locus_tag="ONT85_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HCR2377674.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(44952..46241)
                     /locus_tag="ONT85_000680"
     CDS             complement(44952..46241)
                     /locus_tag="ONT85_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCR2377675.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFINATATDVDTVAAALLYAWILSPVLKAF"
     gene            complement(46268..46570)
                     /locus_tag="ONT85_000681"
     CDS             complement(46268..46570)
                     /locus_tag="ONT85_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCR2377676.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            complement(46595..48688)
                     /locus_tag="ONT85_000682"
     CDS             complement(46595..48688)
                     /locus_tag="ONT85_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HCR2377677.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKVFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            complement(49177..49941)
                     /locus_tag="ONT85_000683"
     CDS             complement(49177..49941)
                     /locus_tag="ONT85_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HCR2377678.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            complement(50052..50921)
                     /gene="mdcB"
                     /locus_tag="ONT85_000684"
     CDS             complement(50052..50921)
                     /gene="mdcB"
                     /locus_tag="ONT85_000684"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HCR2377679.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            complement(50946..52022)
                     /locus_tag="ONT85_000685"
     CDS             complement(50946..52022)
                     /locus_tag="ONT85_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HCR2377680.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            complement(52046..52639)
                     /locus_tag="ONT85_000686"
     CDS             complement(52046..52639)
                     /locus_tag="ONT85_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HCR2377681.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            complement(52643..54319)
                     /gene="mdcD"
                     /locus_tag="ONT85_000687"
     CDS             complement(52643..54319)
                     /gene="mdcD"
                     /locus_tag="ONT85_000687"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HCR2377682.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            complement(54309..54608)
                     /locus_tag="ONT85_000688"
     CDS             complement(54309..54608)
                     /locus_tag="ONT85_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HCR2377683.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            complement(54710..56356)
                     /gene="mdcA"
                     /locus_tag="ONT85_000689"
     CDS             complement(54710..56356)
                     /gene="mdcA"
                     /locus_tag="ONT85_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HCR2377684.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            complement(56363..57274)
                     /locus_tag="ONT85_000690"
     CDS             complement(56363..57274)
                     /locus_tag="ONT85_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HCR2377685.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            57394..58251
                     /locus_tag="ONT85_000691"
     CDS             57394..58251
                     /locus_tag="ONT85_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR2377686.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            complement(58438..58800)
                     /locus_tag="ONT85_000692"
     CDS             complement(58438..58800)
                     /locus_tag="ONT85_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HCR2377687.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            59146..60585
                     /locus_tag="ONT85_000693"
     CDS             59146..60585
                     /locus_tag="ONT85_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR2377688.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            complement(60592..63144)
                     /locus_tag="ONT85_000694"
     CDS             complement(60592..63144)
                     /locus_tag="ONT85_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HCR2377689.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMAFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(63258..64589)
                     /locus_tag="ONT85_000695"
     CDS             complement(63258..64589)
                     /locus_tag="ONT85_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR2377690.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            complement(64555..66033)
                     /locus_tag="ONT85_000696"
     CDS             complement(64555..66033)
                     /locus_tag="ONT85_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR2377691.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITLLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            complement(66338..68173)
                     /gene="tdc"
                     /locus_tag="ONT85_000697"
     CDS             complement(66338..68173)
                     /gene="tdc"
                     /locus_tag="ONT85_000697"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HCR2377692.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
BASE COUNT        19472 a        14592 c        11707 g        22661 t
ORIGIN      
        1 taaaaaaagt tttataaaaa ggtataaaac ctttttatta aactatttaa tcttgtttag
       61 tatatatttt tagaaaaagg agtggggttt ttatggcaga aatcgtcaaa gtcacacatt
      121 tgaaaaagag ctttggtcgt ttcgaagctt taaaagatgt ctctttcacc gtacattcag
      181 gtgaagtcgt tggatttatc ggaccaaatg gttctggaaa atccacgacg attcggacac
      241 ttttagggat tataaaaaaa gacggcggaa cggcaacgat ttttgggcaa gatgtatggg
      301 aagatagttt atctatccat gaacgtcttt cttatgttcc aggcgatatc tctttgtggg
      361 gaaatttgac tggaggagcg atcatcgatc tcttcatgaa gcttcatgga aaagggagtg
      421 ctcaaaaaaa agatgaactg attcaacgtt tctctcttga tcctaagaaa aaagcaaaaa
      481 actattcaaa aggaaatcgc caaaaagtgg gcttgattgc tgctttggct gttgattcgg
      541 atctctattt atttgatgag cctacttctg gtttagatcc tttgatggaa acgattttcc
      601 aagaagaagt tgaaaaattg aaagaacagg gcaaatccat cctactttct tctcatattc
      661 tcagtgaagt cgagcgacta gcggataaag tcgtgatcat acgcgaagga acagtcgtag
      721 aagaaggaac actcgatgaa ttgcgtcatt tgactcgttc gaccatcact atagaaacag
      781 aagcagagaa tagtcactta gcacaattga acggtgtcca tgactttgtg caaaaaggtc
      841 acctcgctca atttgctgct gataacgaag ctttcgatgt gattttagct gaagcgtcca
      901 aattaggtgt gaaaaaattt gaatctattc caccgactct tgaggattta ttcatgagac
      961 attatgaagg atgaggagga agcgaaatga ctgataaatt ttcacgctgg tccacccttt
     1021 tttctcaaaa ccttagccgt gactggaaaa agatcctgat atggctagtc ggtgtcactg
     1081 ctttttctgg tggttttgtc cctgcgtttg aagaattgac ttccggacaa ggattagctg
     1141 gattatacga aaccttgcaa aacccggcaa tgattgcaat ggtcggacca actccagctt
     1201 cgaatgctgc tgagtatact ttaggagcga tgtatgcaaa tgaaatgttg cttttttgca
     1261 gtttgtttgc tgctgtgatc tctgttttac acgtggtgag ccaaacgaga aaaaaagaag
     1321 aacttggtct gattgaactt attcgttcct ttcgagtcgg acgtcaagca aactctttag
     1381 ctgtcatcgc tgaaagtatt ctgatcaatt tgcttttagc aatatcgatc agctgtacga
     1441 tgattttttt ccaagcagat tcgatcactg cagaaggtgc ttggcttttt gggctatctg
     1501 taggtagtat ggggattttg gggagtgccg ttgcactgtt tgctgcacaa ctgatgccaa
     1561 gttcagccag ttcgaatggt ctgtctttag cgtttttagg tggactttac cttctacgag
     1621 caataacaga tgtagctgat tcctcccttt cgatgtggaa tcctttaggc tggtcttatt
     1681 tgacctatcc atttacaact aacaactgga ttcctttgct ttatttgttt ttgttcagtc
     1741 taatcttgat aatcagttca tttgttttag aggaacatcg agatttgaac accggctata
     1801 tccccgaacg tgaaggaaaa ccgtatgcta aacgttctct tttatccttg cgtggtttat
     1861 tattctatct aaataaaggg atgacaatca gttggcttat cacttttggt ttgatggggg
     1921 cggcatacgg ctctatctac gggaatatgc aacagttttt agaaagtaac gacttaatga
     1981 aacagatgtt tatccaatct ggcgtctcaa ttgaaaaaaa ctttacaagc acgatcatgg
     2041 tcgtcttgat ttgtctagct gctattttac caattgctag catcaatcgt ttatttacag
     2101 aagaaaccca cggacgtatg aatcagctgt tcgcaacaaa aatttctagg aaccaacttt
     2161 attggaccaa tatgttacta gctattctat ttgctatatt ggcaatcctg attagttctg
     2221 gtgcactagg gctttctgct ttggcttcga tgaaggatgc gaaagacatg gatctattga
     2281 tgtttcttaa agccgggttc aatttcctgc ctgccgtttt agtgtttatc ggaattagtg
     2341 gctttctatt cggatggctt cctcgaatgg gtaaacttgc ttatatctac ttagcttact
     2401 cctttgcact caattacttt ggcggaattt tggatttacc agaatggttt tccaaaacag
     2461 ctgtccctag ctgggttcca cggatgccaa acgattcttt tgatctttcc gtgttcctta
     2521 cgttatcatg tatcagcctt gcactgttcg tccttggttc tattggttat aggcgaagag
     2581 acttactaga aggataataa aaaagcatgt acaatttcat gctagcctcc aaatctgggt
     2641 caaaaatcca acatttggag gtcatttttt tatttacaag tcttctaact gctctttatc
     2701 aatgatccag acttctcgat tctttctttt tacataacca ttctcctcta ataatttaaa
     2761 ttttctagat aaagtttcgg gggtcgtacc gataaatgca gcaagttctt tcattttcat
     2821 tggcaggaaa aagtgatcct tccctgtcgc tttagctaag tcaagcagat agacagcgac
     2881 tcgttcttct actttttcca tcatcaaaaa agttgtctgc tgctgtactt ttgttaattt
     2941 ctcaacgttg attgctaaaa gtttcagact aagggcggga taatgcagta agagttccga
     3001 gaaatctttt tgtctcaata tgcaaatttc cgttttttct agtgcttcgc caaataaaga
     3061 ctcatttgga acgcctagca actggctctc tccctcatat ccgcccggct ctattactcg
     3121 taaaagctgt tcctttccgc tagcagataa ttgatacact ttcatactgc cggaggccac
     3181 gatcactaat tgaggttctc cattcggcgc aagaatctgt tctccttttt ccacatagtg
     3241 atgtttgaca agtgcgtgta ctttttcttg atcttgctgt actaagtgat tgaacaaagg
     3301 aacaaatgaa acacataaat gatcatgact cataatctct cctccttcta aacagctatt
     3361 tctaataaag aaacataact tcccttattc caactgataa ttttctttta tcatagcata
     3421 aacgtcaagc gaaccagata ccagcaaata gagtggattt ctttttgtcc cttgctttat
     3481 tttccttctt ttgagacctt aagtaattga ttcctatcaa ttttttaaat atttactttt
     3541 tgaataatca aaaacaaacc ttcactcgca agccgagagt aaggtttgtt tttcgcattg
     3601 cttatttcgt gttgtctatt gaaatttttc cgcacctagc ttttgatata gtggtgctag
     3661 ttcttgtaag gtttcagcgg ttatttgctc aacgtgttcg atttcttgcg gatagaagaa
     3721 tagtttaccc atttgaaagg ttcggtctct catcgctttt ttcaactctt cttgtttctt
     3781 tgtatcggac aaatagtcag acaaagctaa gtggtcgtca agttcatgtt ctggttgcgg
     3841 ccatatgtaa taatccttga tatcgactgt tttgctccac tctttcagta tcgcaagcaa
     3901 ctggttatac gctgcaatgc taccatatcg ttcttcgctt ttataatgtt ggtacatcaa
     3961 atagatttgg tattgttttt gattcaacaa aactgctaga caggcattct cgtcttgacg
     4021 agattcgctt cggtacgcgc tccaaaaatg actgcgtaaa ttccagccat tcgtccagct
     4081 ttcgatttta ggtttgctca tgccgtacat atcaggcaaa agtgcagctg tctggagctg
     4141 aatctctttc catttttgcc agtgttcttt atattctttc ttgatttgtt caatcttatc
     4201 tgttggatgg ctctctttga gctgtttgaa tgcgtagatt ggtcgatcaa acaactgata
     4261 atctgatttt ccaagttcca ttgtttcgcc tctttccgtt atcttttgta ttctacctga
     4321 attttgcgat atggacaaat agaggttgtg aaaaagctgt tctgcatcaa gtcataagaa
     4381 cagccaaaca aaaaactgcg atgagcatca ccgcagtttt attgaactta aggttgattg
     4441 atcacatcta aaagcgttga aaataaaatg gcactagaag cagctcctgg gtcgatatgt
     4501 ccgattgcac gctctcctag ataggaagct cgtccttttt tagctaccaa gactttggtg
     4561 tgttcttttg cctggtcaat gatttctttt gttaaattcc ctgccttcaa tgcctctgtg
     4621 actggcagcc aaacatctaa catcgtttta tcgtcagctt ctgcctttcc tcggctttga
     4681 atacccgctg ttccctgctc gatcacttcg cctaattttt cttgagaatc aatagaatcg
     4741 acctcttttg tcgctttcat catgttcatg aatgcagatc cgtataacgg accagaagcc
     4801 ccaccgactt tcgatatcat cgccatagag aatactttga atgtatcgct gattgtttca
     4861 ggatgatcgg cttgaaaagc ttcttcataa gctgaaaccc ctcttcccat gttatttcca
     4921 tggtctccat cgccaatcgg ggtatctaga tcactgagat attgtttgtt ctgattgatt
     4981 gcctctgcaa attgcgtgag ccattcttgg atttcttgaa cggttagttt catattatca
     5041 cccttttatc ttgagatttt ttaccatgaa atcgtctgta cattgctttc taaagaatct
     5101 ttccattgtg tctccgtcaa gtcgatcagt gttagagaaa gaccttgcat atctagtgag
     5161 gtcataaaat taccgacttt tcggaaaact acattgacgc ctctttcttc caacagagct
     5221 aaaacatcat tcatgaacac gaactgttcc atcaaaggcg tcccgcccat tccgttgact
     5281 agtacacctg cttcttttgg taaattcgag taatcatcta aaatcttagt aacaagttcc
     5341 tttgccagta ctttagaagg ctgcatcttt tctcgacgat agcctggctc accgtggata
     5401 ccaacaccat actcgatctc atcttctcct agttcgaatc ctggtttccc tacttctggt
     5461 acagttgctg ctcgaagcgc tagaccgatc gtcttagttg cctgtacgac cttttctccc
     5521 agttctttca actcagctag ggaagcaccg tttcttgctg catcgcccac gattttgtgt
     5581 acaaggacag tacctgcaac tccgcgtttt ccagcagtat acgtactatt ttcaacggcg
     5641 atatcatcat ctacgatcac actttctact tcaatatcgt ccatagcagc taattccttt
     5701 gccatatcga agttcaatgc atcccctgta taattcttaa cgatcagcaa aactcctttt
     5761 ccactatccg cttccttaat agccgtctga atctgatctg gagttggaga agtaaacaca
     5821 tcaccaagca ccgctgcact taacatccct tcccctacaa acccggcatg ggatggttca
     5881 tgtccgcttc ctccaccaga aaccaaacca acttgttccg ctttctgatt cttggctaca
     5941 acacgggaag attctacacg atgaacaagt tctggatagc tttttactaa tccttccagc
     6001 atttcttcga caatattgct tggatcattc atgatttttt tcaatttatt cccatccttt
     6061 ctttccggca ttttgtgctt ctgttaagat ttgctgagta tcatctgtta taccggcagt
     6121 gatggctgct gcaaaagctc cttccactaa aggcgcatcc accagatgat aacgttgctg
     6181 ctgctcttct tccaacatgt cttttgccag ttccgagttc aataccgcgc taccgatatc
     6241 cgcaaaaaca aaataactag ctgcatcgct tgattcattg actgcttcta cgattttcat
     6301 cgggtctgat ccgatctcgc catttattcc gccgagagaa aaaatttgca cctcttcgga
     6361 tttttgcatc tgctcaatca tttctttaat accatctgtt actttttgac tatgagaaat
     6421 aagcaaaata ctttttttca caagaatctt cctttctttt acatagttgc taacgattgg
     6481 tttcgctttc attaatgaac aattcaagta tataataaat ttctaatttt gggaagatat
     6541 gtgtagtgct tcttgaagtt tgttatactt taattaaaag agaggagtga tcgaatgatt
     6601 acaacaacaa cgaacagtgt agagggaaaa gccgtagtag cttataaagg gatcgttttt
     6661 ggtgaggtca ttactgggat ccatgcattt aaagacttgg aggcggggtt gcgaaatatc
     6721 tttggcggcc gctcaaaaag ctatgaaaac gaactaatgg gtgcaagaga agaagcactt
     6781 gccgaaatgg ccgaacgtgc aaaagcatta ggagctgatg cgatcattgg cgtaaaaatg
     6841 gactatgaag tattaggcag cgacaatggc atgctgatgg tcacatgtag tggaacagct
     6901 gtctccacga gataagtagg ggacatctgt tttaataact aaagagagat gggacatgta
     6961 gtggcactca agaattagat ttttcagtct aacttcgagg tactctacct tcccatcctc
     7021 tcttttttat ttctttttgc ggatgattcc gtaagctgct gcgactaaca cacccgcccc
     7081 tgctaaggaa taactgacag aagctgtttt ttcatttgtt tttggcaaag tctgacttgt
     7141 tgatttggat ggctctgtcg tttgctgaac tgtttctgtc gtgcttccag aggtcactga
     7201 tttttctcct gccgaatgca aaatatcttt tattcgttgg aaactagctg ctaagtcagc
     7261 tttttttgct tcaaaatcat tagcaactgt ttgtgcttcg ctgatctttc cgtggattgc
     7321 ttgactggcc gctgtgatat ccgcttgagt ttgacttagc tttccagaag ctgtttgagc
     7381 aacctgattt aattgctgat tgaattcttt tgcttgttgg atcttcccgt caagatctgt
     7441 ttggacagct tccgcttttg ctttcgcttc ggcaagcttt ttctcaagtt gtgcacggtc
     7501 atttcccact ttatttttaa gatcagctaa atcttgatcg attgctgctt ggtcttcttt
     7561 caatttgttt tttgcagctt ctaatgcttc ttcgagttct tttcttttat tttccaagtc
     7621 ttgcaaatga tcgatttggt cagaagaggt agaactattt attgatgtat cttctgaaga
     7681 tgaaatcgtt tctcctgaat tatctgtcgg tacattggaa gtcatttctg tttccgaggc
     7741 accgttttca gcaccagaag agcttatagt tgttgaaggt tctgtttgag caggttcaga
     7801 agtttccgca aaagctgtct gtcccaataa gaaaatactg gaaacagcaa ttggccagat
     7861 taacggcttc attctttttt tcatcacttt tcccatcctt actacttttt aactaatttc
     7921 ttattataag caaaaatgtt tggaagaacc ataaacacaa caggctgttt gtctaaaaaa
     7981 cggtgcatct tttatttttt tgtacgttcc gggcttttcc atgtcccgct tccataacct
     8041 aaaatcgttt tgactgctgg aatgaaagtc gtcacgatcg ttatcgcgtt cactatccag
     8101 aacaaaagca tatatagtgg tgcgaaaaga aggtatttca attttcttcg tcgatcatct
     8161 acaatcagcg aagctagcaa ctggaaaaat cctgcgacca tttcaaagca gatgaacaca
     8221 aaactgaatg taaaagtaat ataaatctgt tcgtagtttc cttgatatac ataatgcccg
     8281 attaatccag caaaaagaac tacggaaata caaaagaata ttgaccagac aatactcaat
     8341 gtttgatcaa tgaagatgat cgttcttcca atatgtttaa aaggatggag catcactttt
     8401 ttgaaattcg tcagccagac ttctgttcca ccttttgccc aacgttttct ttgtcgatat
     8461 agcatcttca acgtcacagg tacttccatg aaaaagataa tctcaggagc aaaaacagat
     8521 aaccagtcat ttaactgatg atcccaagca atactgatgt cttctgtcgc tcgatcttgt
     8581 ctgaatccgc ccacatcgat caaggcttct tttcgataca tcgtatttgc tccactgtac
     8641 gcataaagac caccgaaaac agctgactgt gttcgtttaa tgatgcccac gatacttgag
     8701 aattcaaccg tttgagattt tgcaatcaat ttggtacgat tttggacatc catatttgca
     8761 gtgaccgcag caatatgtct tgcaccaggg cgaatgaaat aattcacata cctaatcagt
     8821 gcatctggct ccggtaccgt gtctgcatca ttgcttaaaa tcaatttccc ttttgcaaag
     8881 gcaagtccga tattaaacgc gtgggctttt cctttatttt tttcgatccg aaccacacga
     8941 agattagcgt attttttcat cagtctagct aatatttctg gtgtttggtc cgttgaacca
     9001 tcgtctgtca ctaatacctc atagttgtga taattcaatt tcgtcatcaa atactcgatt
     9061 gtatcttcga tcacaatttc ttcattatgg gctggcacca tgatcgtgat gaacggttcg
     9121 acactcaagg gaacatctac ccattctttt tgcttatgtt tgtataaaaa cacataacac
     9181 cagacaccaa taaaccatac aaatccgcct aaaatcggat aagaaacgag aaaaaataca
     9241 taatagacaa acaagtatct aagaataaat tacctgtatg ggtgattgag atcatttcag
     9301 ttccacccca ttttctttat acaaatctgc aatgaatgtc gtatctagat ttttctcttc
     9361 ggagacagaa taaaaacgga ccgtttctct ctcttctttt gttccaaaac gttccgtata
     9421 cacttcttca agtaattctt gtcttttttt caatttttca tcttcatatt caaaagattt
     9481 atttactttt ttgtatagat ttcgattgtt ccacctagtc aatacaaatg aggtaatcag
     9541 gaaactgaaa aaaatcaata caaaaaagcc gattaagaaa tacagcattt tcttttctga
     9601 taaaaaagcg tacaaggaaa aatccttggc tctttctggt ctcgttaatg ggaaaaagac
     9661 ccatataaaa ggaagaacaa cgatcatcca tcctaaaaga gccactaacg tttgccaaat
     9721 ctttaaccag taattacctt ttgaaaaata cttatctttc accaaaattg atgcaacttc
     9781 ttctcttgtt acttttttca tatcctcacc tcacgttttt ttgttacgac gtaggagttt
     9841 accatacatt tcatttcttt tcacataaag tgttcctaaa tttttcctaa gaaaaagaac
     9901 aaaacatttc tcatcagcaa atgaaaaata ttttgttctt tttatcagtt gttcttttta
     9961 ttttttatct cttaattatg ttgctctttc caatacgaca atgcttttca ttcctctaga
    10021 tatcgccgtg tacatttgtc tgcgtcttct ttgctcatct ccatcgtttt caaatggata
    10081 taagattaca tgatcaaatt ccagtccttt tgctagactc aaaggaatga tttggcagtt
    10141 ttctaatcca tatgaatttt ttttaatcga ttcttgtagc gtttgggttt cctcttctgt
    10201 ttgggtgata actgccgctt tttctccttt tagagaaacc aaaatctcag ctagtatttt
    10261 caacaattct tcagaggatc gaaaagagcg gtattcgggt tcttttcctt gcggtcggat
    10321 cgatacgatg tcgatcttct cttgatccac agcataggtt ttgaaaagct ctgtgattgc
    10381 tccgctagaa cggtaactat ttctaagatc ataccgatgg atcggcagat ggtagtcgtc
    10441 gaaacaacgc ataatatcag caaaagtagt ggaagaatta aagattgcct gattctcgtc
    10501 acctactaaa gtaaagtgag tttttggaaa taattcgatc aatagaccta attgtgccag
    10561 ggtataatct tgtacttcat caatcaatag atagcgaaat gctggtacat ttaccttttc
    10621 aacaagtaaa tgacgaatag ctaatataat gactgcttca tcaacagtac gtgtgggttg
    10681 ccaagcataa gctgagccat atcgtttttc ataaattctt tcaaacaact gatgctcatc
    10741 cacccatgcc atttcttcga tttgtctggt tatttttcga tatttttttt gtaaaagttt
    10801 ccttgcataa gctacaatac tttgttccga atcatcagaa atcaatttac cgaataattc
    10861 caactgccta tcttctgtca acgagagtac ctgattctgc acagcaggac tttttgcttg
    10921 tttcaaaaga tgattcttcc attcgctcat cagtgccttt ttcgttgctt gaagcctatc
    10981 gatcaattta ggatgagcag gaactttctc atatattttt tcgatcgttt ttttagaaag
    11041 gactgttttt ccatttttag taagtccacg gataaaattc ggatgatcta aaaacaaagg
    11101 atctgattgt tttagattat cgataaactt tttgctacgc aaacgttctg tctgctccga
    11161 tacattttct cccgtgatac gtttgaaata agcctcttca ccttctattt cctctgctga
    11221 aagctgtgaa accagctgta tgatggttag attcagtgga tttctttcac ctaatgatgg
    11281 taggacattc gaaatatagt cgataaaccg atgattaggt gatagaatca gcaagtcatc
    11341 ggatgtcatc gtatctcgat ataaatagag cagatacgca atacgctgca tgatagcaga
    11401 ggttttacca cttcctgcta cgccatttac caagatattt tgacttttca catcccgaat
    11461 gattgtgttt tgttccgatt ggatcgtagc agtgatagcc ttcatttcat tggtctcatc
    11521 ttggcctaag acttctaata atacgtcatc ttgaatagct acagacgtat caaaaaactt
    11581 gaccaaacga tctctttcca aaagaaattg tcgtctttct ttaataaccg tatcgatttt
    11641 attttgacga accgtataag aagatggacc aatcatgtta ttgtaaaaaa gttcggcaat
    11701 tggtgaacgc caatcataaa caagattttc atctgcatca tctgtgaaat ttgctgtacc
    11761 gatataaaag gattcttttg gctcattatc cagaaagtct acaacgactt ttccaaagta
    11821 gggacttttc aataaacgtt cgatctttgc tagctgattt tctgcgtttt tctgtttgat
    11881 attcatctga tcgatttctc tatttttcat ttcaatcatc gaaaaagtat ccaggttatc
    11941 taagtaactg tcaaaattca atcgaagctc gccagccatt tctttcaacg actggtttcc
    12001 ttctattttt gccgtatcca tcgattcccg cagttcgttt tttttatcaa ttagcttttg
    12061 atacacgttc ttcagatatg cttcttctaa ctgtttttgt gtgttgacca tacacattct
    12121 cctctttttt taaagtggtg gacccgatta gtttacctct tttaagagtc gaatgcaagc
    12181 tttttaacga gtatcatttg ttaggatata tcagttcttc aggtcggaca gtttgatata
    12241 aagaaaatgc accatcaagg ttttttacgt ggtagccaga ctgcttcaag attctttccg
    12301 ctagatagct acgtaatccg ctatgacagc tgacgatata ttcttgttgg ctgtctaatt
    12361 catttaatcg gtcccgaagt tcattcaaag ggatatgttt ggcttccttg aatttgccct
    12421 tttccaattc ttctgctgtt cgcacatcta ataaaatttt tccttttgct agttctgtcg
    12481 gcaattcatg ccattgtacg gtttcactaa gcccttctgc aatgttcatt gctgcatatc
    12541 ctagcatgtt tactggatct ttggctgagc caaatggcgg tgcatacgtc aattctaatt
    12601 ccggtaaatc gaagatcgtc aaatgccctt tgattgctgt tgctaaaata tcgatccgtt
    12661 tatccacacc ttttgcccca acaccttgtg caccataaat ctcgccagtc tctggatgga
    12721 agatcaactt cagtaaaata tctgttgctt ctggataata gcttgcatga tcttttcctg
    12781 atacatgaat gacttcaaaa gacagttcgt tttctttggc tgttctttca cttaaaccgg
    12841 tggatgcggc agtcattcca aacgttcgta taattgctgt tccaataccg ccttgatttc
    12901 ttcgagaaat tccggaaatc gtgtccgcaa cttgtctacc ttgacgattg gctggtgaag
    12961 caagagaaat aagtgcatct tttccagtga tttgctgttt tacaacgata gcatccccca
    13021 ccgcataaat atcaggaata ttcgtttgat aacgttcgtc tactaggata cccccgcgta
    13081 tgcctaattc gattccagcg gtcactgcta gctggttatc aggacgaaca ccaatggata
    13141 aaataactaa atcagcaggg atggtacgcc catcatccag tcgcaatcgt ttcccgtggt
    13201 cttcaaatcc agcgacagca tgagaagtaa tcacttctac tccttgatga gacaattctt
    13261 tttctataaa agctgccatt tcttcatcca gtgttggaag aatatgtggt gcttgttcga
    13321 tcaccatgac ttctaaacct cttcttttta ggttttctgc catttccaat ccgatgaatc
    13381 ctgcaccaac gatcacggca cgctttggct gtttttctaa tgcatgcatc actttatcga
    13441 tatctggtac atttctaata gaaaaaacat tgtcggcttc tgttatccct ggaatcgatg
    13501 gaacaactgg ttttgcacca ggagataaaa ccaatgcatc gtactgttca atgtgttttt
    13561 gaccctcatg tacgacttct accgttttgt tttcgggaaa gatggctgtc acttcatgat
    13621 ttggacgcac atctaaacaa aaacgagcgg ataatgattc tggcgtttgg acaagaagat
    13681 tttcccgttc actgatttct cccgaaagat aataaggcaa gccgcaattt gcaaaagaaa
    13741 catacggtcc tttttcaaaa acaacgattt ctgcatcttc cattaatcgg cgaagccgtg
    13801 tagcagcaga cattccacct gctacacctc cgacaataac gattttcatc ttatttccct
    13861 cctactgttc gatcaaacca ttgattcatg ccaccacgta cattcactac atcataacca
    13921 gatttcttta attctttagc tgcttgttta ctcctcatcc ctgattgaca aatcacatag
    13981 acggtctttt ctttgtctcc atcatatctg tttatacttt gtaatggaac atttttagct
    14041 ccttttatat gcccgccccg gtattctgca ggtgttctaa catcaagtaa cgctggattt
    14101 tccaaaagct tttcctttaa ttctgggact gttatcgatt ttattgattg aaatagcaaa
    14161 ttcattttct gcacctcatt tacattttta taaatacctc atggggtata ttaaacagga
    14221 gctttattaa tatgtcaacc agaatgactc ttcatttttt tcactacttt ttatatttgc
    14281 ataaaatacc gataaggggt attatctaat tataaaaata gaggagaatg tgttatggag
    14341 attgataata aaaatatcct gaaccgtttg aaacgtacag agggacaaat tcgtggtatc
    14401 caaaaaatga tcgaagacga aaaagaatgt atggatgtca tcactcaatt aagcgctgtt
    14461 cgttctagta ttgatcgcgt aatgggcatg atcgttgcag ataatctgaa aaattgcttt
    14521 gagaaacctg atagtaatcc agaagaacaa gcggctaaac tagaacaagc gatcaagatg
    14581 attattaaaa agtaaaggaa tgatttcaat gaaagtcgtt ttccatattg atgaattaga
    14641 aaaatggtca gaaacaggga aaaacgtaaa aaacctgata aaagcttctc cagaaacgac
    14701 gattgtcgta tctgtaaatg gcatcgcgat taccggttat ctagactctg ccaatgcaga
    14761 attcttagat ttgcaaggag tcgtttttca cgcttgcgcc aatgctatgc gtgctaatca
    14821 tatttcagaa agttctttac ctgaacaagt aatcgttgta ccagcaggtg tccttgattt
    14881 agttgaactg caatcccaag gttatgctta tatcaagcct tagtcagtag aggttgcgac
    14941 agaagtgatc agcttcaaga aatttcggct tatttttcga agaagctact tctggaaaac
    15001 cgtgaatcaa aggttgtgaa aacgctgtcc tgcatcaagt aataagaaca gccaaacaaa
    15061 aatagcttct catatttttc gtttggttgg gcttattacc gcagatgcaa gcttttgaac
    15121 accgtttaag gaagaggttg tgaaatcagc ggtttgacct aagtattaga gaaaaaaacg
    15181 ggaaaatagc tcctcatatt ttgccgtttt ttgagctaat accgaaggtc agctgattga
    15241 acaccgttta aggaagagat tgtgaaaaag tcgcttagtt tcgagtatta agaaaaattt
    15301 tgtgaaaatg gatttctaca tttttgcata aatttggctt aataccgaga aactggcttt
    15361 tgaataccgt ttaaggaaga ggttgtaaaa tcagcggttt gacctgagta ttagagaaac
    15421 aaacggaaaa atagctgctc atattttgcc gttttttgag ctaataccga aggtcagctg
    15481 attgaacacc gtttaccaag aggttgtgag cctaccattt aactccgaat cacaagaaat
    15541 aatttcaaaa acaacttctc ctgtttttgg tttggttggc ttattatcga gaagtagcta
    15601 ctgggagtct atttatcaaa aaaaggaatg cgacataggt cacacccctt ttttattttc
    15661 tttgaaacat ctttcggtac tcgacagcat acaagacaaa agattgtaaa agaccgtaag
    15721 tgatgacggc aaaaatgact ctcacccaca gcgttggtaa ttctgggaaa agcagcaaaa
    15781 ggatcatgcc aacatagatg acagcagtcg ctactttgcc ataccacttc gctccactta
    15841 atctgatatt tttagatagc ccgattaatc cagctacaaa cataaagcct tcttttatca
    15901 agaatagacc aaacaacagc caaagccacg gtcgatacca tgccatagac aaaatcagaa
    15961 aaagttgtgt caatttatct gcaaacggat caagcacctt ccctaagtct gttacctgat
    16021 tatatctccg agcaatgacc ccgtctagat aatcagacaa acctgagaca acaatgaccg
    16081 ctcctgctat ataaaatgac tgtctcagca caaaataaag ataaatcggc agcaaaaaaa
    16141 tacggaatat cgataaaaga ttcggaatcg tttgtatttc tttacgccag tctgtgctta
    16201 atttcatttt atcgccctac ctcctcagca ctcttttctt tttattttag cttacacggg
    16261 gaataaaacc aatgatttca ctgtttttat ttttctttac agccactgat attatgagct
    16321 aatttgagat aatcacttgc tgttttcatc gtttactctt ctttcttcct gtcgtttgcg
    16381 catttctttt actagtactt tatgagcttt ttttgatcct cgctctaggt cacgctgccg
    16441 tttacgtgtt ttataatcga taaacagact gtctaaatcg taacctgtcg ggtacagatc
    16501 ttctgctgaa cgcaccagac gaacacgctg acgaggcacg tggattattt ttttgtctaa
    16561 atatacatca atcgtatctt cacctatatc ttcataaact aacgcttctt tttggtattc
    16621 attgacaaat atccgatcac cttttgcaaa gagctggcgt tcttttttat tctccttcct
    16681 atttgtaact gctgtcttag gaagaaattc ttttttctgt gtccgatagg ctttttccgc
    16741 caagtaacgc tctgcttgat ggatcaattt catcgacata tccattttat gagcaatcca
    16801 caaagcttgg ctttctcctg tttctccaac ccgcaactga tatttaggac gaagtgcttc
    16861 ccgatcgaaa gccatagcag ccggcacaaa atcttcatga tctccggcga atttcttgat
    16921 ttctccataa tgggtcgtgg caatgatcaa tgccccttgc tcatacatag attccatgat
    16981 tgcgatcgct agtgcagccc cttcatttgg ctctgttcca ctgccaatct catctaaaag
    17041 taccaacgta ttccttttta tctttttcaa aatagcagcg atgttttgca tatgaccaga
    17101 aaatgtactc aacgcatttt ctaaattttg gtggtcaccg acatcgacga aaatctcatc
    17161 aaagacagca agctctgtcc cttcttttgc cggaacttgc ataccgaaca tcgccatcaa
    17221 agtcaataaa ccgactgtct tcaacacgac tgttttgcct ccggcatttg ctccggtgat
    17281 aatcaagcct cgatagtctt taccgatttc aacatccaat ggtaccgcat gctcaggtaa
    17341 aaatggatgt ctgccttgct tgataatgat atgttccgat ttattgattc taggtgtgct
    17401 tccattgatt tctcggctaa attttgcccg agcaaagatg atatctaaca ccgtgatcgt
    17461 ttcgatcaat aaatcgatca cttcttcgtt ttctgcgata gcaccagtca atgccgccag
    17521 gacttgatat tcttcactga tttcttcagc ttttaacaac tgatagtgtt cattcaattt
    17581 agaaatcaca gttggttcaa taaaaaccgt cgttctttta tttgattcat cgataatcgt
    17641 tccatctatt ttatttttat agctggcttt gataggaatc gtatagtatt cgcctttttg
    17701 aacaatcatt gcttcttgga tcatttcttt gtttttcgga tgacgcaaga atttcagtaa
    17761 ttttgactgg atttcttttt cgattagttg taattgtttg cgtactttac gcaaattacg
    17821 tgacgcgtcg tcactgactt tattattttt tattttctgg tcaatcaact cttctacatt
    17881 tatcaaatca ggaagatgtt tgctataagc aaacaacaag ggtgtctggt attgattctt
    17941 atcaaaaaac tttgtgatca tccgactgct tctaagaaaa tccgcatatt cgatcaaatc
    18001 ggttggctgc agaatcaacc cttttttgac ttgttctgtc agtgcatcga tcctcggcaa
    18061 ccccataaat ggaacatgct gactgctttc taagatcaag cgtgcttctc ttgtttcttc
    18121 ttgacgatcc aagacagttt gtaggtttgt cgagaccgtc atatcgctga ttcgtttttt
    18181 gctatagttt ccaattgctc ttgcttggat ttcctttttg atccgatcaa attgtaattg
    18241 actaatcatt tcgttgtcca tattctctcc ttttcttgaa gagatacctg tcccaaaata
    18301 aataagggcc tagagataaa taaaaggata gaacacagct taaaaaagtc atgtcctatc
    18361 cttattagaa tacaaagata ttgttttcct gacaggtacc gtttcataag cacaccaaat
    18421 aaactttgtt tattggctat tcgacttact gattagatac cagctacact cacaaaacat
    18481 accccattct tctaggtctt atttattttc cacttaacta tagcaagaca cattttggtt
    18541 gtcaaacttt agtatgtttt atagatttac accattaatg aatagacacc taccgcgata
    18601 attgctccga tcattggacc tgcgattggt acccatgaat atgcccaatc tgaagttcct
    18661 ttattagcta ttggcaatac cgcatgtgct aatcgaggac caaagtcccg agcagggttg
    18721 attgcatatc ctgttggccc tcctaatgaa agaccgatgg ctaagatcaa tccacctaca
    18781 gcgaatacgt tcgttccagg tgcaaattcg ttttgtccaa aagctagcaa cccaagaact
    18841 aatacaaatg taccaattaa ttctgtgatc acatttgctg gataattccg tatagcagga
    18901 cctgttgcaa acgttcctaa aatcgcgctt tcatctttcg ttgcattcca atgtggcaaa
    18961 taagaaagcc aaacaagaat ggcccctgca aaagctccta aaacttgcgc aatgatgaaa
    19021 ggaacgacaa gattccaact aattgcccct gtcatcgcca tagctagaga gactgcagga
    19081 ttcaaatgag ctgggcccat gaatccagaa atatagacag ccattgttac agcgagtccc
    19141 caaccaaatg cgatgacaat ccagccagaa gcctgtgctt tacttttgtt taaattcaca
    19201 gccgcacaca caccatctcc aagcaaaact aggatcagtg tcccaataaa ctctcccatg
    19261 atttgagtca tatcactgtt catttatttc tcccccttta gttcccgcag atctgattct
    19321 gcaatcaatg cttctaaacg tttagtctgt tgcgccttct cctcttctgt ccattcaaaa
    19381 tatccagcaa ttgcatcgat cacaggttgc ttgatctcat caagctgatc tcgttcaaac
    19441 aacagatgat ttgtacgacg aatgagataa tcgcctgggg ccaaaaccat ttcttcttct
    19501 aaaccataac gtaaccgagc tgactcagcg agactcaaac caggataagg tgccatttct
    19561 tttgctaatt caaagatacg tcgggcatta gtcccgtaaa aatcagcgat ataagtagca
    19621 tcttcttcct ctaaaccggc tgctactcct tcttttgcta attctgtcac tgtttcttct
    19681 aatttagttg gatcgaagtt tccacctgaa atctgatatt ttttagagtc gatttctttc
    19741 gtctctattc cgtattcttc ttttaataat tggcgtatca atcgcaaagc tccttctgcc
    19801 atcttacggt aatcggtgat tttcccacca gataaagtca gcaaaccatc tggttctctt
    19861 tctaaagaac tacctctgga aatcgtagaa ggtgcttttt catcacggct gttttccaaa
    19921 tggttcaaca cgtcttctac ttcagcacga gaaactttgt tttccttata ttcacttact
    19981 gtatcgacaa ctttattgaa gctcttgtct gaaatcgatc cattatctcc accattgtaa
    20041 tcagaaccag aattaccaat caacagtgga cgaagtcctg cccagcttgc ttcgatatca
    20101 ttcaatgtga tatttgcttc tggatagcga tgattgatca catccaatag ataatctaca
    20161 tcttcttgtg tgactttcgg atccgtaaaa tctccttggt aatccgtatc tgtcgtacca
    20221 aagtaagtct tgttttctct tggaatagca aaaaccatcc gcccatcttg ctttcctgta
    20281 tcgaagtatg tgggttgcgg tacaggcagt tttttcgcat ctacaactaa atggatccct
    20341 ttagttggac gcattttagg agagactgga cgcgtaaaat tcaagtttcg tactttatct
    20401 acccaaggac cactcgtatt gatcactaat tttgctttga tttcgatcac ttcatctgtc
    20461 agcaggtcac gagctttgac gccaacgatt tgatcgccat catataaaaa gccaaccgct
    20521 ttcattttac ttactagata agccccatct tcggcagcct ttttgatatt atcaatcact
    20581 aaacgggcat cattgttgcg gaaatctaga tagacacccg cgccttttag cccttctttt
    20641 ttcaaaaacg gttctctttc caatacttct tctggtgtca gcgtataatt ctcatatttg
    20701 gttcctgtca catttgccag tttgtcataa aggtccattg ctactttgac cgagaacata
    20761 ttgaaggttg ttgctccttc gccctcatag attggcaaaa gcattggatc tggttttggg
    20821 atgtgtgggg caatactttg tacgactgca cgttcaccaa ctgtgtcagc tactacttcc
    20881 acatcaaatg tcttcagata acgaataccg ccatgaacaa gtttggtcga gcgagaggaa
    20941 gtcccttctg caaaatcctg catttcgatc aatcctgttt tgattcctga tgctgctgac
    21001 tggactgcca caccggcacc agtgattccg ccaccgatga tcaacagatc caattcttct
    21061 tgctgcattt tttgaatgct atcttgtctt gtcttgtttg aaaacatatg gatttcctcc
    21121 ttattcaaat gcttgggtag cttttactgc ttttttccaa ccgttgtaaa gtttttctcg
    21181 gcgctcttcc tccatttgaa cttcaaatag cttgccagct tcataaaatt cgcggatctc
    21241 gtctgtatct ttccaataac cgacagctaa tcctgctaaa aatgctgccc ctagcgccgt
    21301 tgtttccaag ttttctgctc gttggatcgg tacattcaag atatctgttt ggaattgcat
    21361 cagatattca ttgtttgctg caccgccatc tactttcaat acaggaatct tgattccagt
    21421 gtcatcttgc atcgtatcga tgatatctct cacttgataa gcaatcgact gtaaagtagc
    21481 tttaatgata tcttcttttg tcgtaccacg tgtcaagcca aacatagccc ctcgtgctgc
    21541 ctgatcccaa taaggagcac ctagcccaac gaatgcagga acaacataaa cctcatcttc
    21601 gcttgtagag cgtttcgctg cgtcttctga atcgctagct ttttgcaaca tttgaagtcc
    21661 atctctcagc cattgaatcg aagaaccagc cacaaaaata cttccttcta aagcatagta
    21721 aacctttcca tttatgccgt aaccaattgt cgtcaataaa ttatttttgg ataattgcgg
    21781 tttttcaccg gtattcatga caataaatga acctgtacca taagtattct tgaccatgcc
    21841 tggttcgaat gccatttgtc cgaataatgc ggcttgctgg tctcctgcca tccctgaaat
    21901 tggtgcttcg cttccataaa aatgataacc ttgcgtataa ccataaactt ctgagttaga
    21961 agtgactttt ggtaacatca ctcgaggaat atttagtaaa tctaaaatct cctgatccca
    22021 atctagctga tggatattga acaacatcgt ccgactggca ttcgagtaat cagtaacatg
    22081 tgcttgtcca tcggtcagtt tccatactaa ccaagaatcg atcgttccaa acatcaattc
    22141 gcctttttca gctcgttctt gcgcgccttc tacatgatca agaatccagc gtatcttcgt
    22201 tgctgagaaa taggcatcga tcactaaccc tgttttttcg tggaaaaatt cttgatagcc
    22261 ttcatttttt aagttatctg caattccagc tgactgtctg gattgccaaa caactgcatg
    22321 atagattgga cgtcctgtat ctttttccca tattactgtt gtttcacgct ggttcgttat
    22381 gccaatccca gcaatctgat ttggtttgat ccctgattcg atgaacgctc cggcaataac
    22441 tgactgtaca gaattccaga tttcatttgc atcatgttcc acccaacttt cttgcgggaa
    22501 atattgcgtg aactcttttt gagaactgcc gatgtgtctt cctttcttgt caaagataat
    22561 tgctcgtgag ctagttgtac cttggtcaat cgccatgata tacgttgatt ctgtcattgt
    22621 gattccctcc attatatgca aacgctttct ttaactactc ttatcatact actgttttaa
    22681 aaaaagaaac tgtcaaaatt ttattctttt tttctttttt tgacagtttt ttattatgaa
    22741 gctatttttt aatcaattct tttctttttt cagcgtctct attcttcttt tcaatcgaat
    22801 aatgtcgtaa ggtgacgcaa attctgacag ataatattca cactgttttt tgtttctctg
    22861 gcaaaatgtg atcaccaaat catattcttt accttcttca taacttccaa tctcaatacc
    22921 gccgattccc ctcagttctc tgatcagata ttgttgaaaa gcaatacgat aaatcggcag
    22981 actttctaaa tcgattccaa tcactactgg ttttgcaata taaaaatcga tcatcaaaag
    23041 aacattggca tactggatca tcaggtaact aagctcacta tcttcagctc ttttcctcgg
    23101 caattgctgc accgctgttt cctgtaattt cgccagtaac tgtgttaagg aatgcccgag
    23161 cattttctgc tgctgttcca aaagccgatc acgctcatag acttcgatct ttccaacaaa
    23221 gaaaaaccat tcattgttga tctgggaaat ttggtagcct actgcctttt cttctttgat
    23281 tttcaaacga cgaggacgat aatgccataa catcgtttcc cgaatgtaag tatctaaaac
    23341 agcggttggt aactttttgc tccgtgtcaa atcatactga taaaatattt cttcgtctac
    23401 tatcgaaaaa gaaacaaaaa agctgtaaaa catcattgtt tcgtatacat tcagttctgc
    23461 tgctgttcga ctaagatgca tcacgatgat tggatcgatt gcttgataaa gccggtccga
    23521 ctgatagagt aatttctttt ctttcaaagt tgcgtacgta cttttttcat tcaatagtct
    23581 ttttttcgta atcccgagcc agcaagcaat ctgtgcttct gctgatgccg taaacgttgt
    23641 ttctaataca cggtccaatc ctagaatgaa cggtcttttt tctggtgtat tttgcaaaaa
    23701 aagcggtcgc tgatcttccg ggatatattg gaatagttca taataaaaat atcgtatctg
    23761 tagctcttct ccgtataatt gaccgttttt gatttgtaat ccaaattcct ctaagagctg
    23821 gttcaattct ttgatctttc gaaaaacaga agactcactg atattaaacg ctgtcgctaa
    23881 tcgaacgatg gatacttgct gatgttctaa taaatagacg agcatcttaa acttcaagct
    23941 ttcctgcaat aaaaacaata gaattttttc caaacttaca tcttcgtcca acacaagcag
    24001 cgcctgatac tcatttcgct gtacttctac ttttttttcc atcattcggc caatcaattc
    24061 aatatcttcc aaatattgat caaacgaggc ttttgacagt tggacatgct ctctcaaacc
    24121 attgatagag gctgttcccc cttgcagaac aaggtttcgc aaaatcagga tctgtaactg
    24181 ctcttttctt tctaataatg cttcaatctt cataggcagt attcgccacc tttgaccaca
    24241 ccatatctgg cacacggtat tctggctgga aaaatagctc atgttcttct aactgttcaa
    24301 acgtccatcc ttcttctact gcaattgtca gttggtcaag aaggtggaac atctcttttt
    24361 tgctttggag ctgtgctcca agaatctttt tttcagaatc aaatagaaat ttgagtttaa
    24421 ctttgctttg atttattgct gtaagcttgt agtctacgta tcctgtttca attttttggg
    24481 gataaaaaat gctctcttct tctgtcaaac ctacgcttcc taagaaatag ccaaaccact
    24541 gattgccgac tggacgatag gttccctgat cctttgtctg tgagccacta attgtcttgc
    24601 tgacatttcg tgctgtacgg acagcgttat ttactagaga aacatacatt ttttcatttg
    24661 ttggacgaaa agttacttgg atcgcatcgc caatcgcata aacgtctttt tgagacgttt
    24721 gcaagtagtc atctacccag atcgttccat catcattcaa tgttaattgt tcttcccaca
    24781 tctgattgtc ggggcgagaa tgattagcca gcaaaacaag atcatagttc tcctttcttt
    24841 tttccgtctc tacaatagcg gtttcatttt cggtcaaaga cacaacttgt tcttctaaaa
    24901 aaagtgaaag cccttgattt ttcatttcct ttactaacgg ttctgtcatt tcaggatcta
    24961 gataacgaaa aagaattgac tttcggcttt cgtaaagatg gatttgtttt tttcttgatg
    25021 ccaacccttc agctatttcg attcctgctt gtcctgcacc aataattgca attttctttg
    25081 cattcatgat cttttgctga agagccgtta cgttttgtat ctttttaact gggtgaataa
    25141 gcgttgattc tgcagaaata tttcgaaact tttgatttga accagtcgct agaatcaaac
    25201 gatcaaaatc caattttttt cctcctgcta aaacgacttt tttttctttc aatcctatta
    25261 ctgtcttttc tgtgtaaaca tggattcgat agcggtcagt cagttcttct ttcgtgatcc
    25321 aagaaagttg tttttcactg gtaacctttc cttttaatat caaattgaac ccgctgggaa
    25381 tccatcctaa cttcgattgc ttttcaatca aatatatttc acttgttggc atcaattggc
    25441 gaagataaat agccgcatga atccctgcaa acgacccgcc gacaattaca atgttcatct
    25501 gcattcctct ttcttataag gtgttctact gctaacttct caatcagaaa acacagtatt
    25561 cataactcgc tttcaacctt atgatacata gcgatgcagc agaatcaacc ctctccatca
    25621 taccgtgttc taggtctgct tctcaataat gtactagtgg acctaattga agagtatcat
    25681 ttctgaagag atgtttcaaa aaatgtactc ttataaaaga aaaaaactcg ccttttacgt
    25741 acctctgtcc aaataggaaa cagaaaagaa cgaaaagacg agttttagcg tgtgtaatgt
    25801 aatagttaga atagacacat catttatttt taattcttta atgcttcaac aactgcttcg
    25861 cctaattgat gagcagattg aggattttgc cctgtaagaa gccgcccatc aatttgaacc
    25921 acttctgaaa aagctgctcc agactgatag tttgctcctt tttctttcaa agcatcttct
    25981 gctaagaatg gtacttcatt cgttgtacca tttgcttcct cttcttcatt actgaaccca
    26041 gtcactgtac gtccagctaa aatagatttt ccagactcat ctgtaactgg caacaatcct
    26101 acaacaccgt gacaaacagc tgagaccagc ccttcatttc gatagatttg tgaagcgact
    26161 ttcccaagat ctattgcttc tggaaaatcc cacattgttc cgtggccacc agcataataa
    26221 accgctgcat aatcatttgc atccacatca tcaggacgta aggtatttcc caaggcccga
    26281 tttctgaaat cttcatcttc ataataggcc caatcgattt catccatatt caaaacactt
    26341 tgcggttcta aaggtacata tccgccttta ggactgacaa aatctgcatc gatcccattt
    26401 tttactaata catcataaaa atgagtcagt tcacttaacc acaaacctgt aggacgctcg
    26461 atcttttcgt acttttctac atttgttaat acgatcaata ctttcatttt cccatcctct
    26521 ttctataaaa attgcgttat tgataacgtg ctttcaagaa attatataat gggcttggca
    26581 aataaagctc tttatatgct tcttcaaaag gtttcgttac aaattcatcc tcacggtttt
    26641 ccaataattt ctctgcccag tcaggatcga cgatcatttg ctgtccaatt gctgcaagtt
    26701 ctgcatattt caatacctgc tctacatctt cgcgattgcg gatcccaccg acaccaacta
    26761 aagggatctt tccttgaatc gtgttatgga ccacttcaag aatcgattta tcgttgtatt
    26821 tatcagaacg agctattcga tcatacatat ttaatgaaac atgcagataa tctagttttg
    26881 actcacgtaa tcgttcaaga agccagatcg tgtcttcaaa acggatacct ggtgtttcat
    26941 attcttctgg tgagaaacga taccctacga tgaatggttt tttagcatat tgttcaaccg
    27001 ctgagaaaac cgttgttaat aattcttcaa taaaacgata acgattttcc aagctaccgc
    27061 cccattgatc ctctcgtcta ttcgaatgcg gagagaaaaa ttgttggatc aaatacgtat
    27121 ttgctccatg caactcgatt ccatcaaatc ctgattcaat cgctctacgt gtggctttgc
    27181 caaatgcttg gattgtttca acgatttctt cttcactcat tgctcgtggt gtttccgcat
    27241 caggacgttt tgctgcaact gcacttgcag atacaggctg ttctccagat aaagttgctc
    27301 gacttgtcat tcgtccaccg tggaaaatct gaataatcgc ttttgcgcct tctccttgga
    27361 ttgctttcgc tagctcactt aatcgaggaa tcatttcatc atgagcgatg ctgagttcac
    27421 ctggccagcc ttttccgata tcttggacat ttgcagcacc agtaataaca gcaccaagac
    27481 ctttagaacg ttgtttataa taagtgatct catcttcggt gatcactcca ttgaaaaagc
    27541 tgagttgtgt cgtcataggc gacagcatca atcggttctt caattgaact cctgaaggta
    27601 aagtaattga ttgatcaaat tgtgacattg tcattcctcc cataattcgc tctgattaac
    27661 ctatcgagaa tcatctatca ttaaaccact cgattagttg tacatgcaac taatttgctt
    27721 aaaaaatttt attttttttg taacttatct gttgcatcat tcattgtctg ggctaattct
    27781 ttacttgctt catttcccaa aactgctttg tatgactgat gaagttcttc ccagctacca
    27841 tcgacttcct tagcaaattg gcgtccttgt tctgtaagat aaactaatga caagcgacct
    27901 tcgctaactg ttttgacgta tcctttatat tctaattttt caacaaaacg agcaatcgtt
    27961 gatggggcgg aatagagcat ttgtgcaata tctttttgtg gtattccatc tttttctttt
    28021 aatatcaata aaataaaacc ataagttggt gccagacctg tccttctaaa cgcttcatca
    28081 gctaatttat tcaacatgcg atctagcttt ttcacgctaa agaaaagaca ttcatctaac
    28141 gctttttcaa ttggcttttc catttcatcc cctccattgg cgtacatatc atagtatgat
    28201 ttttattttt tgtcaaaaaa cacggaataa tgtccgcctc ctctgtctag atttgaaaaa
    28261 aagaagtagc catcttcaaa gaatttcggc ttgttttcga caaaactcct tctggaacac
    28321 cgtgaatcag aggttgtgaa aaagtcgttt agttttgagt atgaagaaaa attttgtgaa
    28381 aatggctttc cacatttttg cataaatttg gcttaatacc gaaaaactgg cttttgaaca
    28441 ccgtttatat cagaggttgt gaaaaagccg tcctgcatca agtaataaga acagccaaac
    28501 aaaaatagtt cctcatattt tgtcgttttt tgagctaatg ccgatggtca gctgattgta
    28561 caccgtttat tgagaggtcg tgagcctgcc gttgaactcc gagtcacaag gagcaatttc
    28621 aaaaaacagc ttctcatgtt ttatgactat ggtgacttgt gatcgaggag ttggcaggag
    28681 aatactgttt attcgtaaag aaggggctgt gacaagactt ttgtcacagt cccttatttc
    28741 cttttattat aggatttcgc catttgattg gatgacttgt ctgtaccaat caaatgattt
    28801 tttctttaac cgtttctttg tgccattccc ttcgtcatct tgatcaacat agatatatcc
    28861 ataacgtttt gacatttgtg aagtagaagc gctgacgata tcaatgcagc cccacgacgt
    28921 ataaccaaat aaatctactc catctttgat agcttccttc atctgctcaa tatgctgttt
    28981 caaatagtca atgcggtaat cgtcttgaac ggttccatct tcttctactt tgtctatagc
    29041 tccgagtccg ttttcagcta tgaacagtgg tttttgatac ctatcatata attgattcaa
    29101 agcaattctc aaacccttag gatcaatctg ccagccccat tcacttgatg gaagatacgg
    29161 atttttaatt cctccagtta ttaagtttcc ttggacccct tcccaatttt ctggatgagc
    29221 actgactgca ctagacatgt agtagctaaa agaaacaaaa tcaacagggt aggctgctaa
    29281 gatttcttca tcacctactt ctatttttaa gttgacacct ttttcttcaa acattcgttg
    29341 cgtatacgct ggataatatc ctcttgcttg aacatcacta aagaacaaag aagttctttg
    29401 ctgctcaatt gttttttgaa tatcatccgg atcacaagaa tacggaacaa gaagctgata
    29461 gctgatcata caaccgactt gtgaaccttt gataatttca tgacaagctt ttgtagctaa
    29521 agcacttgct ataaactgat ggtgtgcacc ttggtatttc gcttgttcca aatggggatg
    29581 gttttcttca attactccta cgctaacaaa agggtgatgt tttacacagt tgatctcatt
    29641 gaaagtcatc caatatttta ctttattttt atagcgtttg aatactgttt cagcgaaacg
    29701 aacgtaaaac tgaataagct ttcgattagg ccatccacca tattctgtaa ccaaatataa
    29761 cggcatttca taatgggaaa gagaaacgac tggctcaatc ccatatttta gacattcgtc
    29821 aaacacttga tcataaaaaa ataatcccgc ttcattgggt gtttcttcat caccatgagg
    29881 aaaaattctt gtccatgcga tagacatccg atagcattta aaacccattt cagcaaataa
    29941 acggatgtct tctttaaagt gatgatagaa atcatttccg tgtcttttag gataataata
    30001 cttgctgttg ggatcctgcg ccgcttttac ttgctgatgc gtcatcatat gccattgatc
    30061 tgtccatttt tctttaggta aatcgctgtc gaatgtatac acatcagaca cagataatcc
    30121 tttttcatct acatcccatg caccttcaca ttgattggcc gctgtcgctc cgccccataa
    30181 aaatccttct ggaaattttt tcatatcgat ccccctaaac ttgctagttt tcttgtttga
    30241 ttatttttct tatgactagt aatgatattt ttgaatctac tactatttgt aacaataacc
    30301 attgtggtcg gatcatatcc ctctttgatt aattcttgca aatctacttc catcaataaa
    30361 tcgcctttac gtactttttt tccatcagat actgcacttt gaaagtattt gccatttaaa
    30421 ttcactgtat caataccgat atggatcagt atctctgctc cttcttcact gactacccca
    30481 attgcatgtt tcgttggaaa aactgttgaa acttccccgt ttacaggact gtagattttt
    30541 ccgtccgttg gaatgatcgc aaacccttct cctaaagatc cactagcaaa tgttggatcg
    30601 tttacttcag ataaatcaat tatttcacct gctgcaggag tacttaacac tatatccttt
    30661 gaggattctt cttcgatttc atccttgtag ccgaaaagaa aagttagcat acaggaactg
    30721 atcatcgcta agaaataact agcaactagc caaacaaatc cttcaccaat ataaatcggg
    30781 aaagacaaaa tacttcttgt agcaactgcc aaagcaccgg ctcctgcggc accagctact
    30841 ccaccaaata tagctccaca tatacaagca atgtaaaatg gtcgtttata tttaagagtc
    30901 acaccataaa cagcaggttc agtgattcca aataatgctg tgataaatgc agataaagat
    30961 aaacttttta ctttcttatt tttcgttttc aaaaataccc caagtactgc tcctgcttgc
    31021 gcagctacgg cagtcataca tgctggacct agcgtatcac gaccgtagaa agaaagattg
    31081 ttggtcatta ctggtacaag tccccaatgt aacccaaaaa tcactaacac ttgccaaaat
    31141 ccgccgataa cagcaccaga taaaacaggg ctaaagttat aaacagctaa atatccagct
    31201 gcaatcagct gacttgcata atctgcaatt ggacctacaa ctaataaagt aattgggacc
    31261 atcacgatga tacaaatgaa tggtgtcaat aaatttcgaa tactttcgtg caagcttttg
    31321 cgaatcttag gctcaatata agaaagaacc catacagcta aaattgctgg aataaccgta
    31381 cttgtatacc ttgctagaat cacgggcatc cctagaaatc gcaaggttat gctatcacta
    31441 tatgcactga caatcgtagg ataaacgaga gctgctgcaa taaccatagc gatgaaacga
    31501 tctgtattga acttttttgc agcagtatat gccaaaaata taggcaagaa atagaaaaat
    31561 gcatcagctg ctgcattcaa aatgatataa gttcctgatt cagtagttaa taaattcaaa
    31621 ctcgtacata aggcgagtac tcctttgatc aaaccacttc ctgccaagac gcctaaagtc
    31681 ggggcaaaaa cagcggcgat catgtctata aaagagttta aaatgccttt tttctcagtt
    31741 cctctctttc cactagatgc atcatcttct agtttcattt gtttggataa tacgtcatac
    31801 acttcattta ctgcatttcc tactacgact tgaaacattc ctccagcttc gactacagtg
    31861 atcacacctt ctagttgctc taccttctcc ttatctgcct tcttattatc ttttagggta
    31921 aagcgtaacc ttgttgcaca atggtaaaca ttgctgacat tttcttcgcc tccgacaaat
    31981 cggacaatat ctttcgctaa ttgtgaatag tccattttta cttccccctt gttaattaat
    32041 gccgtagcaa tccttcttac aagataagta tagttcttag aaaacggttt ctccattcaa
    32101 ttagtacccc ttataatagc gaacgaaact gtacaatagt gtgtatggtt tacttagccg
    32161 agcaaatcac tcactgcttg aaagaaaaca agtatatcgt ttctcttata gtcaaaggag
    32221 acaaaaaaaa cacgcttgat cttcctttgc tgatgtataa gtggtatctc aagctgatac
    32281 tctgtgattt ttgtttctac ttggcttttg ataagacagc acaacaaagt ctcggcattt
    32341 aacggaagca taactaggtc agaactagga gagattagtt gctgaatgga atctatcttt
    32401 ttatggattt ttatttgatt ttcttctttg tagtaatgat caagcagttt tgttttcttt
    32461 ttttcttcaa ctatggtcag cttttcacgc aaaatctgat aaatctccca agattcaaac
    32521 tcatctagaa cactaggtag ataaatcaaa tcatcccact caaaaataag ctcttgctca
    32581 gtagtcaaaa ttgcatcata ttccgttatt ttgtttgatt gatttttgaa aagatacact
    32641 ggttctatcc ggcattcttt gatccacatg ttcatacttt gttgaatctg tttttttagt
    32701 gtattcaaaa tactaattgg ctggttacta acaatcagta tctccccttg ttttcgaaaa
    32761 ggattcaacg cttcggcaag atacaaaaca aaataagcgc tttcagctaa attgggacgt
    32821 aattgaaaat gtattgggaa gaaacgccga attaaataaa tttctaaagg ataacgggag
    32881 atgatctttt gtacaaaatg attcatggta ttatgtccgg catccatcct cgttttcatt
    32941 tgtttgatat gggttagcaa aaattctgtt tcatggaaca ataacgaaac aggaagatct
    33001 aaaaaacgaa taatcgtttg ctcaaatgct cttagattct tctctatttc tttgtttttt
    33061 tcatcattaa tcatcaaata atttaactct aatagtcgat tttcaattgc ttgtttttct
    33121 tgattggaaa aagaataggg tagttctgat gataactttt ttgtgagggt ctctaccatc
    33181 ggaataaata atgtatacct gtttgttttt tctaaaagtt cttctcttcg aagaatggtc
    33241 aaaacgagat accgagaaaa tcgtatgaag tcctctcctg aaacaatata attatatgat
    33301 ataagaacct cttttaatgt cttttggaca gtcggcatga tattggaaaa aattgtactg
    33361 atttgttgag gcaaccttgc gtgttggatc accgtttctg ttccaacaag agataagaaa
    33421 attctttttg aattttcttc agcaatgatt tttagtcctt ttgtcgacga aacttccagt
    33481 tctaatttag gattgcgctc gatccaacgc aaaatcgttt ttatttgtaa tgatacggct
    33541 gtttttgaaa gaaagaaaat gtctgctatc ttttgcatac tgacataccc tttttgaaac
    33601 aataagtacg tcacaatagt tgaagctcgg aaatccatgc tggaatagat tttatgtttc
    33661 gtcacctctt gtatgatttg ctgtttagtt tcagattcaa ttgattctaa aaaataacct
    33721 gaacgtttat ttgactggat aatgattttc ttctcgtaga tagcaagcat atttttgatt
    33781 tcagtttgga tcgttttctt gttggtacct aaagcttcag ctaattcttc gcttgttatc
    33841 caatgattct tatttcttaa tactacaaga atttggagtt gtcgtttatt taagtccatt
    33901 tgcttacacc tcctctttgt tataaacata caggaaaaac ctctcaaatg acaatagtca
    33961 cttgcagagg tttaaaaatc atttgaagaa agatctatat tcaaggtttt taccattaga
    34021 ctggataatt tgtttataat attcaaaact atcttttttc aatctcctaa agcttccgct
    34081 cccatcattt tgtctatcca cataaataaa gccatatcgc ttcctcattt cccctgttcc
    34141 tgccgatacg atatcgattg gtccccacca taagtaacca atgatttggc aaccatcttt
    34201 tattgcctca gcaatttcct gaagatgtat ttttagatac tcacaacgtt ttggatcgtg
    34261 gattcgattc aattcatctg gtgcttcatc tagtccaatg ccattttcaa ctatgaataa
    34321 tggtaattca taacggtctg tcaattcatt catgacataa cgaaacccta gaggatcaat
    34381 tggccaactc caaggttcgg gagatttttc cttcaaaaag ggattatcga aaccatttgc
    34441 tccgccagta tctacgcgca tttctgcttc ttttttatag acagcgctac gataataact
    34501 aaatgcaata taatctgccg tgttagcttg caagcactgt tcgtctccat ctaacattac
    34561 tggtgcacag tcttcttcct tccatgtcct gtaaatataa gctggataat gccctcggca
    34621 catgacatcg gagaaaaacc atgaattgcg tttaaattgc agtgaaccaa atacattctc
    34681 tggatcacag tcatatgcat aagtcgctaa tgcagaaaag ctacacataa tacccatctt
    34741 agcatttgga tcgattttct ttaataattg agtagcacgg gcgttcgcta gaaacatatg
    34801 atgacaggct tgataaatca ctttttttgt tagtgtagca atgggttcaa gtttatctct
    34861 agcagggaat gtaaccaatc cagcagctgc ataaggcata cgaaaagcat tattgatctc
    34921 attaaaggtc atccaatatt tcactttccc ttggtagcgt tcaaagacgg tctgcgcata
    34981 aaattcaaaa aagtcgatta attttcgatt ttgccacccc ccatactctc ctaccaatgc
    35041 taagggggtt tcataatgac taagagtaat tactggctcg atatcatatc gatttaatgt
    35101 attgattagt ctgtcataaa atacgagacc agcctcattt ggcttctgtt cgtctcctct
    35161 tggaaatatt cgtgcccatg agatgcttgt tcgaaaagct tttaaaccta actccttcag
    35221 taaacgaata tcctcttcaa atcgatgata aaaatcaatc gcttcatgag aaagataatg
    35281 gagtgattcg tctaaagcaa tctcccaaat ctttttttct tcattccact gaatactagg
    35341 atgctggttc atgatcccga taagcgtatc tgtcacatta ggtaatttgc cgtcttccaa
    35401 ccatgcacct tcacattgat tagcagctac tgctccgccc cataaaaaat tttctggaaa
    35461 tatttcctga tccataacta tctctcctcg ctatcaacaa tttcttactt gaagtatagc
    35521 tcgattcatc aaaacaagat attctaacta tttcggcaga agagaagaaa aaaattgaat
    35581 gctactcttc atttttaata tttatatata acaaatacat aagacgatcc ccgaaagaaa
    35641 acactcgtta accatctaag caaacgattt attttacttc caacacaaat gcttcataag
    35701 gtcgtaaatc aactttgccg tctggtaact gtttcagagg ataatttgac acaataaccc
    35761 ttaaagcttc acgtggaaca gtgaacgtct gcttttcact ggacatatta caaataacga
    35821 gccattgctg gtcttcatat atgcgagaat aggcaaagac atgctcaggc gtcttttcca
    35881 ctaattcata gtctccccag acgatgatgt catgttttct acgcaaatcg atcaatttct
    35941 tgtacatata aaaaacagaa tcttgatctg ctaacgcttc ttctgcattg atttctttat
    36001 aatttggatt gactcgaagc catggaattc cagtagtaaa cccagcatgt tcacgggcgt
    36061 tccattgcat aggcgtccta gcattgtctc ttcctttcgc attgattgat tccaagattt
    36121 cctcttttgt aaatccagaa gaaatccttt cgttatacat attgattgtc tcaatatctt
    36181 ttgcttccgc gatatcatcg attgggcaat tcgtcatgcc taactcttct ccttgataga
    36241 tataaggggt gcctttcatc atatggagca agatagcaaa catcttggca ctttcttttc
    36301 gatacacatc gtcatttccc caacgagaaa caattcgcgg aagatcgtga ttattccaaa
    36361 ataaactatt ccatccttca tcacctaaag atgtttgcca tttggataat acttttttca
    36421 gttcagagat actcaagggt ttcaaatccc atttgtcttt gccttcttgc tgatctaatc
    36481 ctatgtgttc gaattggaag accatggata attcatttct tttaggattg gaataaagtt
    36541 tagcgatttc ggtagttgct ccccatgttt cgccaacagt catcagattt ttgtcaccaa
    36601 aagttgcttg attcatttcc tgaagatact catgcagttt aggtccatta cttgtgattt
    36661 ctttatccgg ttctttgcca atcatatcga tcacgtccat acgaaagccc ccaatacctt
    36721 tatcgatcca gaaattcatc atttgataaa tctcttggcg aacctgttta tttttccaat
    36781 tcaaatcagg ttgacgcttg ctatacaagt gcagaaaata ttgccccgtt ttttcatcca
    36841 actgccaagc agaaccactg aatattgagc gcaatctatt aggctctttt ccattgaccg
    36901 gatcacgcca aatataatag tcccgataag gattttcttt atttttacgg gcctcaatga
    36961 accacggatg ttcatcagaa gtatgattca ccaccagatc catgatgata cggatatttc
    37021 ttttctttgc ttcttcaatc agttcttcca tttcttccat cgtaccgaat tctgccatga
    37081 ttgcttgata attactgata tcatacccat tgtcatcatt cggagactcg tatactggag
    37141 aaagccaaat tgcatcaata cctaaatacg ccagataatc caacctggaa ataatccccc
    37201 gcaaatctcc aataccatct tgatttgaat cttgaaaact tcttggatag atttgataaa
    37261 caactgaatg gtgccaccat ttttgttcca tgattttcct cctataaatg gatgtttcta
    37321 ttttttgctt aatcatttat tttagtctct ctcactaaaa attgtaacgc tttcaaatag
    37381 ctattgcacc ttgttatcgg taacagaata aaacaaaaaa acagctgagg aaattccctc
    37441 aactgttttt tgaaaggatt gatttatgca gggaagaatg ctgatttcac aacttcttta
    37501 taaacggtgt tcactcagct gtcccttggt attagctcaa aaaacggcaa aatatgagga
    37561 actatttttt cgtttttttc tctaatactc aggccagaac actgatttca caacctcttt
    37621 ataaacggtg ttcactcagc tgtccctcgg tattagctca aaaaacggca aaatataaga
    37681 aactattttt tcgttttttt ctctaatact caggccagaa cgctgatttc acaacctctt
    37741 tatttcacat tcaatatcca catgaatccc acaaatacaa agaacagacc atacatgatt
    37801 ggagatactt cttttcctct tttagctgct aacatggtaa ttggatagaa gataaaacct
    37861 aatgcaatcc catctgaaat actataagtc aaaggcatgc cgatcaagat caagaatgaa
    37921 gggatcgcaa tctctaagtt tccccattcg atttgtcgca aagattgtgc catcaatact
    37981 cctaccataa tcaatgctgg tgcagttact tgtgaagtaa caactgatag taatggagaa
    38041 aagaacaagc ctaacaagaa gaaaatccct gtagtaatag ctgtgatacc tgaacgtcct
    38101 ccaacggcga taccagctga tgattctaca aatgcaccaa caggagaagt accaaataag
    38161 gaacccgcaa gcatagctgt tgaatcagaa gctaaagctt tccctacacg aggcatcttg
    38221 ttgtctttca tgaaacctgc ttggtttgct aatccaacta aagttcctgc tgtatcaaag
    38281 aaagtcacaa gcaagaatgt cagcactacg acccacattt gaagagaatt gatgtcccct
    38341 acatgtttta atgcaactaa aaatgttggt ttcaaacttg gtgctgcggc aacaatttca
    38401 gaaggcattg gaatcaaacc tgtgaccaga cctaaaatcg ttgtagctgc tatcccgatg
    38461 aagatgccac ctggtacacg ccgaacaagc aagatagcag ttacgactaa tccaaaaatc
    38521 gtcaaccaag tagaaccaac gttgagtgat cctaatccta ctaatgtaga ttcattcgaa
    38581 acgatgatcc caccttcgct cagaccaagg aaagcgataa atagaccgat accgccagaa
    38641 atcgcatatt tcaaatctgc tgggatcgca tcaatgatca tttcccgcag cttgaagatt
    38701 gtgatcaaaa taaaaatcaa agaagcaacg aatacgccgg ctaatgctgt ttcccatgga
    38761 atccccattc ctaaacaaac agaataagca aaaaaagcat tgatcccaag agcaggtgcc
    38821 gttccaattg gataacgggc taataccccc attaaaacac agccgatcgc actggctaat
    38881 gcagtcgcag taaacactgc tccttcatcc attccagaag cacctaatac agttggattt
    38941 acaaataaga tataagccat tgagataaat gttgtaaatc ctgccagtac ctcacgtttt
    39001 accgtggtgt tcaatttttc tatttcgaag taggaaagta ttttttcctt catcttccat
    39061 ccccctaaat ccgaacatta taaataaagt ccgttaaaat ttcgatttat taataaaaca
    39121 acgtgtttta tcatatacta aaacaaaaca taagtaaaca aaaaattcgg attttaagca
    39181 aaatccgaac gttccatctg attaaatccc tttatctcaa agttcatgaa cattcacttt
    39241 ggattacaag aatttcttta ttgtgttaat aaagtttctt cattctagtc tcaaaaatca
    39301 atagtgtgat ttgattttac catttcgttt ctgttcttct ttgatgaatt cttttgtatc
    39361 gacaggattc agattattta gtccgccaaa acctgtatcg accatttttc cccagccttt
    39421 tgtcttcttt tttcctgatt tgagattctt tgctcgaagc ttctcactat gttttgctcg
    39481 ccgatacttc aaataacctt ctaatcttcc cattagatcc actgtttttc ctccttttta
    39541 ttcaatcaga cgactcagct tttcctataa caaaataatt gccttcaggg tcagaaaaat
    39601 tgaaagtcaa gatgccattg ttatctgtga tttctcctgg ttcttccatg ttctcgtaga
    39661 tagtttcaaa ttcatccgta aaaaatacta aagaaggcgg tggtcctaat acctctggtg
    39721 aaaacttctg cacaaatact tttgggaata cgcctaattc taattctttt gagatgctga
    39781 gtacaatact cttaaattct tctggcaatt cgattgtttc agaaatttct gcaccaattt
    39841 tttctacaaa gaatcggcaa atcatctcta cgtcattgac ataaagcatc acacgtgttt
    39901 tttgtatcat catttgaccc ttctttctat ttgattttct ttcctgtcta taaactgatt
    39961 ccattatatc acgaaagcgc aaacaccttg tgtttgatac tattcaagaa aaataaagca
    40021 atagtttcta ggattgcttt cctccacaaa ctttttttac aaaaaaaaaa aacggcaatg
    40081 aatgccgctc ttcttatttc gattctttta taattctgct tctgattcat ctttggttag
    40141 tgtgtgtcta acactagtta cgccaaatgt ttgatcgatt ttttccttca acgcttcgat
    40201 tgcctgtgcg ccacgtgccg cttgttcagc attgatggat tcaatgacca gcaccgctgc
    40261 ttgtgtttct tccgtcagca ttgttctttg ggcttcacgc cagttgagac agcgacagat
    40321 cgctccttgt tcatcatagt agatgatttc ttccggaagt gccggagcat cggaatctgc
    40381 acctaaaggc aggaatgatt cgccaccttg ggcttttcct aaatgcagat ttcctgcgat
    40441 cttttcgata tcttctccgc cgcaaggtac gccaaaagac aaggaaatac tattatagat
    40501 atctacaagc ggattgattg ggaaaaattc ccgttcttga tttgcacgtt tcagtaaagc
    40561 ttcaatagaa gaacgtgcac cttttttagt cttgaatgtg gtaaatgcct gacgccactg
    40621 agcaatgact tcgttttggc tgaatgtttc ttctgtaagg taatttcttg cttcttttgc
    40681 tgcctcattc aataattgat gatacgtttc ttcactttct tttgttacgt ggttatcgat
    40741 accatttacg gataagattt cgatggttgc ttcaggaaat acttcccaga aattggtatc
    40801 cactattact tttttcatct gttcacacct cttgattaga ataattgctt gatgaataga
    40861 tagtctgttt ggggatcatc tcccatataa aaaacatgtt cgctgtattt gataaatcct
    40921 tttttacgat aaaaagcgag cgcaacatcg ttgtgttccc aaacaccgag ccaaatggct
    40981 gttttttgct tttcttttgc tttttcaata gctgtatcta gtaaaaatgt accgatacct
    41041 tgatgtttaa atttttttcg tacatagatt cgctcgattt ctaaaaaatg atcagccatt
    41101 tcctcagatt gactcgttcc tgtatttagt ttcaggtaac ccgcaggctc ttgatcgata
    41161 ctcactagga aaaaatcggt ttctttatct tttagctctt ctgtaagttt ttctagtgca
    41221 taagcttgtg tcaagtaatc agctaaatgc tccggtgaat tatcttttcc aaatgtatct
    41281 gtaaatgttt cgatactaat ttgttgtagt aagtctacat ctgaaggcgt catttttcga
    41341 atttcgtatt tattcacggc ttcttccttt caatagatcc ttttattgcc ttttttgaca
    41401 aaattccaat cttcgctaac attcttgcat actttattta aaagatattc caattgtttt
    41461 gcttcttcgt cagaaagacc atttaatgcg actgcattgg agtagttgtt ttctcgaatg
    41521 atgaaaggat agatttctgc tcctttttta gtcggataga gtttcttgat ttttttattt
    41581 tctttatctt ccaaacgttc gatcatccca ttggattcta atttcttgat tgctcgggca
    41641 gtcgttgtac gatctacttt gatcatttct gctagttttt cttggatgat tccaggattt
    41701 tcgcaaatac gtactaaata aagatactgt cctcttgtta aatcgacttc tttgaattcg
    41761 atattgctaa tagaatctag cgctcgggca atcattccga tgtctcgaag gatttcagcc
    41821 atttcatccc acctttattt tgtttgtaag actagtttac ctctttttgt tgcaaatgca
    41881 actattttca ataaaaaaat caagttcttt tttaataact agtaaagtat attcttttct
    41941 tcttttacga aaaagctcaa tcacaataga tctggttttt gttttctaaa ataagtaact
    42001 gtttcttcgt tgttcgcttt tctgatagct ttggcaactc ttctatacta aaaaatccca
    42061 tgttggaagt ttcattattc tcaataaact tggcctcttc accgtggatc gcacaagcaa
    42121 aaacaagttt ataatactga aaaagctgtg gaatatcttt ttgtttattc gtatcaaaaa
    42181 ctgctcgcaa ctgagtagtt tctactgtta atcctgtttc ttcatagact tctttacgca
    42241 cattttcttc aggagacaat cctatttcag caaatccacc aggaagtgac cattctttcg
    42301 tccgtttatc ttcgattagt aggaccttgc catctttttt gatataggca cgaacatcta
    42361 cttttggtgt tggatgtcct tctccaatcg ttgtgagcgc ttttatttct tcgaaagttt
    42421 cttttcctat ttcactgatc agttccaatg aaatcgtccg gatctcctca aaacgctctt
    42481 tatcaaatac atcttttcca taatatagac cagcttgaga aagtgcgagt agtcttttgt
    42541 attgagccaa cttcttgttc attctcttcc cttctttttt agcaaatgta tgactatgta
    42601 atcaagtata gaagactggt ttaattacgg caaataaata taatgaaatc tgcaataaat
    42661 aaaagaatgt taaaaaactc gagaacaaaa gacattcgac ttctctgggg ctgtgaataa
    42721 atggcgttca aaagcttgca tctgcggtaa taagccccac caaacgaaaa atatgacgag
    42781 ctattcttgt ctggctgttc ttattaattg aggcaggaca gctttttcac aacctcccca
    42841 ctaaacggtg ttctcctacc aactcctcgg tcacaagtca caatattcat caaacatgag
    42901 aagctgtttt tttgaaattg ctccttgtga ctcggagctg aacgacaggc tcacgacctc
    42961 tcggtaaaca gcgttcaaaa gccaattttt cgatattaag ccatttcttt gttacttatt
    43021 gtcattgatt tctttttcta atttatccaa tagtggagtg taatccactt gagtatagtc
    43081 tgttacatca aattcatata gtgttcctaa ctggaatttg tcatattttc ggtcggcgat
    43141 gatctttttg aatgcttctt tcgtaatatg attggtccct ttcgttcgat cttccaatac
    43201 gtcaccatag tgataatgat ccatgatata gctcaaatga cgatctgatt cacgatcacg
    43261 tttgtaccga cgttgacata acacttcaaa gtctgctgtt aggcgaatcg tgatcacctg
    43321 atatccgtat ctatctgcta attcttggag tcgtgctttt tgcttatcac taaaaggata
    43381 ctcggacaca atcactttct ttcctgcttc catatacaaa tcaagtgctt tgtaatagaa
    43441 aggataaact ttcgtccttt cttgttccgc ccgttcttcc aaattgttaa acccgatgga
    43501 ttctgcatag tattccttga tttcatcgag cgtgatggca tacatatctg gaaatcgttc
    43561 ccgtagttta ttcataagat atgtttttcc tgtaccagga ctaccagcca gaagaatgaa
    43621 atactttttc atctcattta tttcctaagt taattggact ttccaagttc ataaacagtc
    43681 caatcttatg cttgacaatt tcttttactg cttcatttgc tggaatcaca ttatggcgaa
    43741 gagacttgtt gatttctgtt tcactaaagg tttctttagc tactttcgtc cagttgacta
    43801 aaagctccgt tccaacattg aatttgcgga tcccgttatt gatcaattca ggataatctt
    43861 cttctttcac acctgttcct ccatggataa ctagggggac atcaactact gcatcgattt
    43921 ctttcagcaa gggaatgttt acttctgttt ttgatttgaa ttgtccatga ttcgtaccga
    43981 tagcaatagc taatgcatcc acgcctgtag cttctacaaa tttcactgcg tcttccggtc
    44041 ttgtatatac cttgtcattt tcagcgacat ggattccttc ttctgtaccg ccgattgttc
    44101 caagttctcc ttctacggaa acaccgtgcg catgggcata ttctactact gcttttgtct
    44161 tcaggatatt ttctttgaaa ggcagatggg acccatcata catgacagaa gtatatccgc
    44221 tgtcgattgc ttctttgata tcatcaaagt ctcgggcgtg gtctaagtgc aagactgcat
    44281 caaccatttc ttcttctgca atagctgtca ttacattgac taacacatgc atgccgatat
    44341 atttagcggt atcgacactt gtttgaataa tgatcggcga acccatttct tttgctgcgc
    44401 ctagcatagc aggaagcatt tctaggttat gcgtattaaa agctccaact gtaaaattca
    44461 actcttctgc tgtttttgtt acttctttca atgttgtata catcttttat tgccacctta
    44521 tctttttatt tgtttccttt gatcgttact tgttttgcaa tttgtcgcat ctcatccatt
    44581 ttgcccatgt agtaatcgat cccttgatca tcaccagcac gagcagcatc tgcacgcttc
    44641 tggttataga ggctcatcag ttttttatac ccatctccga tcgtctgttt attttccaaa
    44701 ctcttttcta aatattggat ggcttgatcg ttcacataca attcagaaaa tttaagtcca
    44761 acttgttcat aaagtttcgc taatttatgc ttatcttggg tagtggaaat ctcttgtttc
    44821 aattgtgtaa tctctgattg aatcttttcg gtcgcttctg gcgatagggc tttttcttct
    44881 tttgccagaa cttttttcgg ttctttttct tttttcttaa aaaatccaaa catcattttc
    44941 cctactttca attaaaatgc tttcaaaact gggcttaaga tccaggcata taagagtgct
    45001 gcggctactg tatctacatc ggttgctgta gcattgatga atcccatcga attaaagatc
    45061 gttactagca aagcgggcaa taatgtaata aagaaaccat gagcaatacc gccaatgatc
    45121 gctcctcggc gtccgccaac tgcgttgcca aatatccctg ctgtccctcc ggcaaagaaa
    45181 ttggttagca taccaggtaa aatcatcgct aagccgaaca tgggcaaagt aaacatggca
    45241 ataatcgtac caatcgttgt cgtaatgaaa cctaaaatga cagcatttgg gctataagga
    45301 aagaaaactg gacaatctaa ggcagggatc gcatcaggaa ctaatttcat agctatccct
    45361 cgaaatgccg gcacgatttc acctaacagt agacgtacac cagctagcaa tacatacaca
    45421 cccactacga attggatggc ttgtaaaaag gcaaacatga tatagttttg atctcctgat
    45481 aattcagaag cgaatggttc gcctgcaaat aaaacagtga tgatatatag cgggaccatc
    45541 acgaccatga cagacaaata agtatcttgc agaaattcga aatgtgtcgg caacttgata
    45601 tcttcgatcg actttttatt ttcgcctttc tcaccaaaaa gcttagcaac accggcttca
    45661 aataagtacc cgatcgtaca aaaatgacct aaagcaatat catttgatcc agtgatttta
    45721 cgaatgatcg gttgcgctac tgccggcata gcaatggcga aacaagctcc gataaaacca
    45781 ccgaccaaga tcaatacgat ccctcgcaga ccggcaaaat agccaaagac tgtcgtcatc
    45841 gttgccatcc ataaaattgc ttgaccagtt aaaaaaatgt acttccattt tgtgaaacga
    45901 gccaaaataa tattgaatac aaaaatggct aagaacgtca gtgcaatatc tctacctaaa
    45961 cccaaatcat tcattgcttg accattgata gattcaatag aagggatgat cccttgcata
    46021 tgaaatcctt cagtaaagat tttcccgaaa taagttaaac ttccaacgat gatgctcgaa
    46081 ccggcactca acacttgaaa tcctaggagt gtctttaacg tgccagagat gacttgtcct
    46141 gctgatttct tctgcagtag tagacccaac atagcaatca atgcaatggt gatcgatgcc
    46201 tgagtcaaaa tattttcgat gataaaattg ataacgccca tgtttttacc ctcttttctt
    46261 tttgtattta gataactccc ttatcttgca gaactggggt gattttttct tcaatttcat
    46321 tttttgaaac aatatttttc aaatagatca tcgccgtttt aggatcaact gcgaactttt
    46381 cgaactgcga acgaaaattt tctgctgtga tgatcacatc tggtttcatg gaaactgcgg
    46441 aagaaatatc acaatggtcc agtttggctt ctactcctaa tttgttcaaa acatcctcag
    46501 cagacatctg tgctgcaaaa ctacttccta aacctgcacc gcatacgaat aaaatgttca
    46561 atttgttcat ctttgttctc ccccgtatca tttattattt ctcaactact gaaaaaagca
    46621 gttgtttgaa atcttcgata tttgtgcatt caatcaatcg atccaacttc tcttcgttgt
    46681 tgatcaattc aattagttct ttcatgatgt tcagatgcga gtaggagtct actgctgcca
    46741 aacaaaaaat aatctttact ggatccattt caggattacc gaaatcgatt ggttgctcaa
    46801 tcgtagctac gctgactcct aatttattga ccccatcttc tggtcgggca tgggctagtg
    46861 ccatgtgttt gccaatcacg atatacggtc cgtaatcatt cactgcacga atcatcgctt
    46921 cgacatagcg ctcttcgaca atgttttctt taaccaatgg tctagcagct agttcaatcg
    46981 attcctccca agtagtggct ttttctttga tcatgatatt gctgtccgtc aaaatatctt
    47041 ttaacatagg ctggatctcc cttttgttga tttctaaatt gtttgtttcg aataattttt
    47101 ctaacttgcc atagatgatt cgatctactc ggccaccact ttctttcatg acatctatta
    47161 tttgagtgaa taactccgtc gattcatgac tgttaggctc taaccgctgt agttcttcat
    47221 ttcttttcaa aaagtcttct ataaccaaga tattcgtctc agttaaaatc ggatcgacaa
    47281 ctaaaagcgg cttatttcga taattcaatt gaaaggtgct gaaaaccaga ttgacatcca
    47341 atttatctac taattcgatt tcttttgaac ttaaaactgc agtaatctcg atctgaggaa
    47401 acttttcttt cagattctct gctaacagat ttccagtagc catcccatgg ttacagacaa
    47461 cgactgattt ataaacataa ttcaagtctc gcttgattac actggcgatc gtcgaaaagt
    47521 ggatgacaag aaaagctatc tcgtcctcca gtatcttgcc accaattact tcttcgatcg
    47581 taggaacaaa atgagtgact gcgcggtaga ttgggaaata atttgttcgg atgttttctt
    47641 tcaatggatt catgacttgg atatgattct tgattcttac aatcagtgca gccatgtgct
    47701 tatagagatt ttcgaataaa gcttcttcct ttagatggaa aggtacgtgt gtctcgttct
    47761 ctacaaattg gatcaattgg atcgttagta attgagcttg tacccactcg attgaattgg
    47821 agatatctct agcattgaac gtctcaatag tgaaacgaag ataatttctt tctacttgcg
    47881 ggacattgtc caattgaaat tcctcgatca tgtggtcaat aaatgcagaa aagctactgt
    47941 cttttatttc tgtatttttc caattaatgg cagagataaa ttcatgtttc ctgattcgct
    48001 ggatccagat caatgtaaaa agcaggattt gttttttata aatatcatct tcttgtttca
    48061 acaacttatt ggaatagatc tcctgtattt ttttgatttc caccaaagga aaataatgga
    48121 gaaagatttt ttgagccggt gttaattgtg catcttgatt cataaagaag tcggtcattc
    48181 ccagattctg attgataaaa tcatagatca tcgtacggat agatcgttct gaacctttat
    48241 aaaccagtcc ttgctttcca taactgacga tctctatccc ataactgacc agtgttgctc
    48301 gtaaccgtct catatcttca tctaatgtac ttttagagat ttgaaattct tcttcttttt
    48361 tgtttagatg gatcgggatt cggctgtaag caacggctaa aactagatca aaaatacgtt
    48421 cgcgccgatt caaaaagccg tctttcacat cttttatttc agtttctaat cgagcttgct
    48481 tttccttttc cagcttcaac aaaaaaactt tgttgcgaat tgtacggatc tccggaagct
    48541 ttttactgct caaccagata ttcgcttcaa gaacttcctg acggatcgtc cgctgactga
    48601 cattgaactc acttgctaga tgttttgctt ttactggata ttgggctgtc atgattctgt
    48661 gaagtatttc aattgttctt gttttcatag aaatgatctc cttctgattt caagtatatg
    48721 gttccgctta cataataaaa agtagatatt tctgccactt gaagtggaaa gaaagtaagc
    48781 aaagcaaaca aaaaataagc catatgcttt ttcatctcct caacgaaaaa gacatacgcc
    48841 tattattaca aaaattttgc ccatctatac aaaaaagagg ttgtgaaaaa gctgtcctgc
    48901 atcaagtaat aagaacaact aaactaaaat agtgcttcct atttttcgtt tggttgttct
    48961 tattatcgca gatgcaagct tttaaacacc atttagtgga gaggttgtga cagagatgtt
    49021 cagccttgag cgataagaag cgattttgaa aaatcgcttc ccatatttta caaaatcgtg
    49081 ccttattgcc gataggctat ctctggcaca ctgtttattc agaaaaagga gctgcgacaa
    49141 ggcttttgtc acaactccgt tcataaatat ttcctgtcaa tccagcataa tcggctgaat
    49201 aaaatcggct tgccatcttt tttctttctc agaaaaatca aaataccata tctgagaaaa
    49261 atactgtcgt tgtgtcttac ttcctgagat caatcgccca tcttttcgat cgactgaata
    49321 atcacagcat ctgaaatcaa ttcggatggc aaaactattt tcgtggatct gtcgataatt
    49381 tcctacttcc tgaatagtta tttttttagt atgatttctt acaccgtctg ctttgttttt
    49441 ttgcagaact ttctgatgtt cggcaaacag cttgtctgta taacagcttc tggctttttc
    49501 aggagtttct tcttcccaag cggattggat cttcatgaat atcttttgga tttccagaaa
    49561 ctgtgctttc ttttctttag ccgttcctga catgcgggac aatatctccg ctttcagcct
    49621 tttcttttgt cttttattct tgacgataga aaaagcagaa acccctccta ctgacaaaac
    49681 agctaatgca agaaatcccg tttgctcata actagaagga cctgtacctg gaccataata
    49741 cccataagaa tctctgccgt aagaacgact gccaaaataa ctgcccccat atgaacgacc
    49801 accaaatgaa cctctgcccg aacttcgacc gctagatgta cctccaccat gaccacccgc
    49861 gacagcgaat ccttgggtcg gcagagcaaa caaacatagg cccactaaca aaatgattgc
    49921 taaataattg atttttttca ttgtcttcct cgttatcttt tattacaaaa aactattttt
    49981 atgatagcct tgctaaaagg aagagtcaat aaccagatgc ataaactgtt tttttagcaa
    50041 ttttgtctta tttagtcatt gagtaacatc ggacaaatgt atgtgtcggc agtccataat
    50101 tcctctatct tcagcatgaa caagcttgca gacaagagat ctgcgcttcc tccaggagag
    50161 atttggtttt cctgacaaaa gtgcaacagt gctgtaaatg gacgatttgg taaagcactt
    50221 tgactagcag catgagcaag tttttgcatt tccactaacg ttttctgatc actgcgatgc
    50281 aatacgcatg tgtcatctag cgaactcatc aaaactaaca acatatgcat ttgtttctgt
    50341 acatatgaat cattatctag ctccttagct gcttggattg caagcctgat atgcggatac
    50401 cctaatatcg cttcttcata tgcaccattt acgccatatt tttgcttcac cctatgacca
    50461 tgagtggttt ttttatgacg atattttgta tcaggaaaag cagcaagctt gctgacatca
    50521 tcaagtattt tcgataaaga aaaaggctgt tccatactga ttttttgtgc aatgacactg
    50581 atcaatagcc ccattgccca aatcgctcct ttatgggtat tgatgccatt tgtagcaatc
    50641 agcattgcct tttccgcttt tcgcccaatc tcagcaattc tttctcttaa ctcctgatca
    50701 atagcatgat gccatgctat ttgagccatt tcttcaaaaa aaggagttag cgtcttagca
    50761 gacttaagaa ataaagcata gtccatatcg gaatgtgcac ccgcatccag tgcatccact
    50821 aatccaggtt ttggacttaa tgttacttct gcgatcaatg cttcctctac caacgcagcg
    50881 agttgctgag tcactgcttt ttttcgttcg tcatttatca tgacactcac tccttcttcc
    50941 tactattact tgctgaaacc ttttttgata gctacccaaa tgatatgaag taaccagctt
    51001 aaagcaaatg cacaacttgc cgtcattaga taagctacaa gaatcatgac tcctgaaggt
    51061 aactgcagcc aactatcttt aaacaataat aacagtactt ggaaaacaaa tacattggct
    51121 aaatacgtac gataagcata aacagacaac catttgaaca atggcaattt tggcgaatcg
    51181 tttacaatca ttttggaagc gcctatgaag acagcaaaga tgatgactaa actgtataaa
    51241 ctttggatcg tattcaagta aggtgcattc gcaaagctca agtctcctgg atgtgcgagc
    51301 aatgaacgga cagaaaagaa cgcgataact aaggcaacag gtaagaaaag ataacgtact
    51361 ctgtccaaat attgatagac tttttcgtga taaacaaatg cggctacccc ataaatcatg
    51421 aaactagcaa caaaccgatc taataagtac cagctgtcat gataaggccc agtcaatact
    51481 tgtgtttgat agaacagata ccatgcgaca tataaaatcg ttgaaccaat caatactggc
    51541 catacatatt ttttattagc aaatactttg taaccgagcc aaacaaaaaa cggcatgaac
    51601 aattggatct gcagcatcat aacggtatac cataaatgag gcgctccatc acctgtgaca
    51661 tatttcagta aaaagcttgc agtatccgta tacggagttt gttgttggac tgctggaaat
    51721 agccaaagat aagccgtcgt ccatagaaga tacggaacga ctagttcaaa aaacttttct
    51781 ttcaaaaatt cccaataaga caaatgctga cttgttttta ccatattata aacaattgca
    51841 aaaataaaca ttggtgctgt gtatttaacg atgacgtaaa aggtaccaag aaaagctgta
    51901 tttgttgcgc tgtggctgtt tgccaaagca aagctcaata tcgtttgcat cataacagcc
    51961 gttacagctg atactttctg gaagtcactt acgttgattt tgattttttt gttttcaaac
    52021 atctctttct ctctcttttt ctttattacc aaagttcttt ggttaattta ggtccttcga
    52081 ctgttttcaa tagaattttt ttctctggct ctctcaaata ctcttttagt gaaaaaccgc
    52141 cttgccgagt cactacttgg gtatcgaatt gctcaaaaaa agcaggatgc gattgaatta
    52201 cctgaattgg caattcaatc ggtgaatttg cgtacagcag caaatcaaaa tcactggttt
    52261 cttttactgc cggaatccca gtcgataatt caaatcctaa gctgccgccg attccccatt
    52321 tacatcctga taatatgttt ctagcagcat gaaattgctg ataaacagga taatccatga
    52381 ttttctcttg tctatacgat tcttgttcga tgatgtccca cggatgaatg acttgtttga
    52441 cggcatgttt tgagatttct gttgaccatc tctgattgcg ggcaggtcca cgtaaaccaa
    52501 tcggtagtat ttctgcgtga tccgtcgacg cacgacgaac tgttccgaac atcttttcag
    52561 ttagaacgac ccattcaggc aaggtgaacc cttctatttc atgtttatca aaaaggacaa
    52621 tatcatgtgc ttgaatcatt ttttatgcat cccattcttc gtccattttg gcacggactt
    52681 tatttgtttc gacacgtccg aatgttttgg ctgtttcgtt tgtgtatcga tttgtcagca
    52741 tcgtgtcatt tgtattgcgg acatcttgaa tggcttcatt gattgcttcg atgactttgc
    52801 tgacattttg ttctgaagca gcttggtcgg tgatatcttc taggaaatga tagatcgctc
    52861 ctagttttgt ataattattg atatcgtaag cgatagctgg aactttttca gcagctgctt
    52921 cgatttcagc aaccgaacgt tttgtcacac gtgcagcact ttctttagac atcgcttgga
    52981 cggtaatttc tttattgttt aaagcaatca agcggcttga ttgtaatcca tgtgctaaaa
    53041 atgcacctga aatagcattt cctgcaatca atccaataac tggatgtcca gcttgacgga
    53101 gttttgcata agcagcagca ctattagcca aagccacatg gataccgatc aattcttcgt
    53161 tatagccgta tgcttggctt ggtacatcga tcacaacgat gattggccgt ttcacttttt
    53221 tgtctttatc ttcttcgtaa acgtgatcca aaatttgtcc catgcggaaa ccttcaacta
    53281 aaccaacttc accgtttcgt acacggtaca tcgggttttt aggatcaggt acgattgatg
    53341 tcacgatggc ttcttctatg aatttacttg atttaccata atacacagta ctaatagaag
    53401 aagttggatt tttcatgcca gttagtgccg caaaccaatc gtaaccaaca cttgctgcag
    53461 ctggttcatt tccttctgct acttctggta agtcaattga ttttgcttga tggttagcaa
    53521 ttgcttcatt gtaatcgtca atggccattg gttttgaaag atctaaagaa tcaagtaatg
    53581 ataaataaaa tgctgagctt tcactgcgat gcgtgtcttt tcgttcgtcc attgctgcga
    53641 tgacagcagc tttgattgct tctacactat cagtcactaa ttcatcaatg atttttgatt
    53701 ctacccgttg acgagatcct aatgtattcc aaatcaagtc tttgtcacta gaatcaaatt
    53761 ctcgaacgcc tgcttcttgt tcaataactt ctggtccatt caaaccgact ctcgcttttt
    53821 ttgttgcgat taaataactc attaatgctg aagtaatcga cataccgcca aatgatccta
    53881 cacgtcctgg aatcaaaccg atgactggta catattgttt tagagcaatg attacatttc
    53941 cgatttctga aatagataac agtccgtagt ttgcttcttg caaacgaaca ccacctgtat
    54001 ctagtacaat gactggaaag atctcatttc cttttttatt ttcttctaac acatgttcaa
    54061 gtgcagcaac gatttttgca ccggaaactt caccgatacc gccaccttgg aattttcctt
    54121 ccatagcgat tactactgct tgtttgccat tgatcgtgcc acggctgaca acgatcccgt
    54181 catcactttc agggacgatt ccttgtgctg gtaaacttgg cgcgatgatt tgatcgaatg
    54241 gatcaatcaa ttcacgtcca ttgtcaagta gagcgttcac tcgttctctg gcgtttaatt
    54301 ctacaaaact atttttcatc tgaaagttcc tccattgctt gcgtcaatcg taggtacaca
    54361 actcctggtg tagcaccgaa gtcattgatc acgatatctg ctgaaattgg ataacgatca
    54421 aaaaatctcg ttaatacatt gttccatact tcgccgaagc catcgcttcc tgtacaaatt
    54481 tttacagtag attctgtttt ttctgttgga aacatcaaga tttccaaatc tcctgaaccg
    54541 accactccta catggatttt cttattgatc ggctgtttcg ttggatattt ataagctaat
    54601 tgttccatgg atctctcccc tttttgtaaa acagagcata cacgttctgc ttattcgtct
    54661 ttcttcttcc atctgggtaa atggcgacaa aaggtttttc tcgccaattt tatgaccaag
    54721 ttctgaattt cattggtggt tcatacaggc catctgacca ttcgatcaaa tcatcgatgg
    54781 tttgtgcagc taataatgaa cgtttcgctt cactacgttt gatgcctaga tcttctggta
    54841 atttcacgat gccggctgca cgcaagctat ctagtgattc tttcgtgcta gtcattccaa
    54901 gaggtgtaac accgccaatt gctgcaattg ctgcttgtcg ttcttctttt gtttttgctt
    54961 tgtacaaata agcaattcct tcttctgtca cgatatgtgt agtatcttcg ctataaatca
    55021 tcactggtgc attggctaag cctgcttgtt tttgaacagc tagtgcatct aattcttcga
    55081 caaagactgg ttttttattt gaaccatatg tttcaaccat ttgaacaact aatttttggc
    55141 ctttccctaa tggatcatca ttgtcgcgaa gtgattgcca tgctggtgta gaatgacgac
    55201 gaccgccagg attatgtccc atgttaggcg caccgccaaa accagataaa cgtccttttg
    55261 ttacggtaga agagtttcct tcgtaatcta attgcaaggt tgaaccgata aacatatcga
    55321 tggcatattg tcctgcagct tgtgataatg ttcggttact gcgcatcgtg ccgtctttac
    55381 cgacaaaaaa gacatctggg cgagcagcaa tatatttttc cataccaact tcgccaccaa
    55441 aactatggat gctttcaacc caacctgatt cgatggctgg aatcatcgtt ggatgtgggt
    55501 tcaataccca gttcttcgcg attttccctt ttaaccctag tgattcacca taagttggca
    55561 gtaacaattc gatagctgct gtattgaatc caataccgtg attcaaagat tgtacgccgt
    55621 gttctgcgta gattcctttg attgccatca ttgccattag aatttgcaat tctgtgatgt
    55681 tttgaggatc tcgtgtgaat aaagcttcta attgataagg tttatctgct ttcacgacaa
    55741 cgtctaccca gtcaccaggg atatctacac gtggtaaaga atcgacaact tcgttgactt
    55801 gaacgatcac gataccatct ttgaaagctg cagcttcgat gattgttggt gtttcttccg
    55861 tattgaaacc tgtgtacaag ttgccgtctt tatctgcttt atctgcagct actaaaacaa
    55921 catttggaat caaatcgatg aataagcgtc cataaagttc caaataagta tggatatcac
    55981 ctaatttcat tgtgccgtct tcaatcattt gggcgacacg caagctttgt gcaccagcat
    56041 aagagaaatc gatcttgctt gcgattcctt tttcaaaaat attcaaatgt tctggtcgtg
    56101 agatactaga catgatcaaa tgcagatcat tcactttttc tgcatctact ttttcaagac
    56161 tttctgaaag aaaacttgct tgtttttgat tgtcgccttc taatacaact ttgtcgccgg
    56221 gagcaatcag tttttccatt acttctacga ttttttcagt ttctacatac ttgccattga
    56281 gcaagttgct gattttttcc aatcgctgcg cttttttttc gcgtttagaa gcccagttgc
    56341 ggtttggttt ttccataatt agtcatctcc atttttgata tagaaacgtg gcgtcatcaa
    56401 taccatgttg gtctagattg atcgtacgga tccctttatc tacaccaaat tttgtatgaa
    56461 tcagctttgt caaagtattg ccggggggaa attcaatcgt cagatccatt cctaattctt
    56521 ttgccaagtc catcatttgg ttccactgca cagggaaagc taggttttcc aacaaatctt
    56581 tacgaatctc ttctgcgtcc tgagtggctc ttccatccgt attttttata tacaaacatc
    56641 tagcatcaga tagatggaca gactgcatat acggcttgaa tttcttgacc gtatcattca
    56701 tcaagagaca gtgagatggc acagggactt tcagcagctt cgctgtttgc gcttggtgcc
    56761 gtttggcttc tgccaacgta acggataaac cttcgatact cccagataac gtatgttgca
    56821 tcggacaatt ttcatttgac agataaactg gatggtcagg atcaaatgtt tttgctacaa
    56881 tttcctcagc ttcgcttctc gtcacaccta ctactacgcc cattgcataa ccgcttggat
    56941 aagcttcttt cattaagact gcgcggtgat agactagttc cgctgcattc tcaaatctca
    57001 aagatcgaca agcaacagct gcacaaaaag caccaatcga atgtcctgct gcaatatcag
    57061 gctgaatacc gtttttttcc atttcatcta aataaaatac tgcttttgtc agtaatgcta
    57121 gctggataaa aaccgtatct tgatagtttt cttctttatc aactaaccga taccccgtca
    57181 cattttctaa acgttccatg tacatttcag ggatatccgc taacatccct aacttctgac
    57241 tgccttgtcc aggaaataag aaacctgtac tcatgagcct cgcctcctca gcacttactt
    57301 tttccactgc tatcttagtc aatttctgct ataataaaaa atgccaaaaa aagaaaaaac
    57361 cataacaaaa aggtatggat gggatggata attatgaaat tgcttcatct gcagtatttt
    57421 aaaaaagtat tcgaaacaaa aaatgttacc caagcagcca aagaattatt tatttctcaa
    57481 cctgccttga gccgtgcgat caaacatttg gaaaacgaat taggtgtccc tttgttttac
    57541 catagcggca gaaacattga gacgaccgtt tatgcagaag aattttatcc atatgcagtc
    57601 aagacattag acacattaga agaaggaatc aaattattgt cttcggtcaa tgagaagatc
    57661 gttacttccg tcatattgta tttagaggta gcctctgttt ctattccgaa cttggtccga
    57721 atttttatgg aaaagcatcc tgatatccaa ttaacgatca tgcaacatca tttaccagat
    57781 gatattaccg atcctgtact gtacatcact tcggagatga agcctggcat gaccaatatt
    57841 cctatcatga aagaacctgt ttatgtcgcc attcctaaga aacatcctct tgctaaaaaa
    57901 gaacatctgc aattagaaga tatccaacgc acccccatct tgatgctgtc aaagagcaac
    57961 gccttccgtc atacactgga tgcagcactt gaagcgaaaa gtatcgaatt gacgatcagt
    58021 tcgacgacag atgatcctgc gacattacgt tctattgtca agcaaggatt agctgttagc
    58081 tttttcccaa aagtttcttg gtcttacgat aaaaatgatc ctttcgtctt gcgggaaatc
    58141 aaagatttgc ccttgacacg aacaatttat ttatcttcta gcttcacaga agatcatcct
    58201 ttgatcaaaa tgattgcaaa aacgatgcgg gaattttatt taggaaaata aaaaaaaaag
    58261 agctgggaca aaattcttgt cgcagctctt ttttcgaaca aacggtgttc cagaagtaat
    58321 ttcttagaaa ataagccgaa atttcccaaa aatttgagaa gcaattttcg taaattcctt
    58381 cttatttctt gaagctaaac atttctgtca caacctcttc tctattgtcc tagacattca
    58441 ttgcgttgat ttaaaacttc catcaatcaa atctgctagt tcatctagct tttctatttt
    58501 acgtagatca atagagatcc aatgttcttt attcatgtga taggctggaa aatacccctc
    58561 ttttcctcgc aaaattccgc caagttcagg atcgaccttc aaatcaagaa tattgatgat
    58621 cctatggtca cttgcatcat catagatttt ttcttctgga acagacatca ggaccccaaa
    58681 ccattttttg cttttttgat gtctgaatac gcaataatca ggaaattttt taaaaatata
    58741 ttctggagac aaattgtatg tttcattgat gtgatcgatt agttcttttt tcgttagcat
    58801 ctgcttccat ctctttctta ttgattcgct ttattcaaag ataccacgta agaatagaaa
    58861 aatgaataca agtagttgct aatcaaatac ttcgatattt ttctttttta tcaactctta
    58921 aatgttcaat ccgcctgtgt tggtcttgct accatctact attaaaatgt ttattattgt
    58981 ttgcccacct attttttgat tttagtggta tcatttttca agttcatgat gaaattctta
    59041 tcatcagtaa atgctccaga aaaataaaca atgattgaaa aatcagttga aacttgacca
    59101 ccgtttattt tctccgtatt cttagaatag aagggagtct gattgatgta ttcactaatg
    59161 aaaaaaatca ttaccgaaaa agatattcga cgtcatgttt ctttagtaga acaattgctg
    59221 aatcatacaa agataaccgt aaatgaatta gctgaaatca tagggacaac cgaacgaacg
    59281 attttttctg accttcaatc tattcgctct cagctgcccg aaggctggga catcatcagc
    59341 gatcaagcag gaatcagttt gcaaaaccag caaaatttat taacaaatga cctttgggag
    59401 atttttttca aacaatcagt cagtgtcgaa ctgctgaaaa atctgctttt tacaaaaaaa
    59461 gttgctgtcc ctgattttct agccgaccat gggctttctt atgggacatt gaaaagacat
    59521 gtgactaaaa tcaatcagcg tttagcaagt tacgacttgc agatcgattt gacgaaatat
    59581 acagcttgta ttcttggaaa agagcgtgcg attcgtactt tttatcatcg attgctgatt
    59641 cctttcactc ataacaacta ttttttcgag gattattcga tccatgaatc ccattatttt
    59701 caatttttaa gaaacctcag tcaaactgaa ctagcggttg aaacagaaga gattttcgga
    59761 acatgttggt tctttatcaa tacgatccgt ataaaagcga attgccgatt agattcttct
    59821 atccatacca acagcacact ctcctccctg tatgactctg cattgaaaaa attgtattta
    59881 acggaaggta tttatcttaa agacactgag ctttcttttg cttctttctg ctttctggaa
    59941 agttggaatt acaacaataa ctatggacaa gaaaccgctc gctgcctgca tcattctcca
    60001 tttttagagg tcctcgaaac gtttgtagag gaactagctt cagagctgtc tcttgatcaa
    60061 ctaaaaaaaa catctctgac tgataacttg gctctcctca tattaaaata tcatgaatcg
    60121 ccgatattga ttgaagcatt ggaccgtcaa taccatcgtt ttttagaaac atatgatcag
    60181 caatatgcac acttgtatca acagaaagag aatttactga acgatctaaa aaaagaaata
    60241 gaaatcaatg aaccaaatta ctttctccaa ctcttgtctc tactgatcca acaaacgatt
    60301 ttttctatca aaccgaattt gttcaatgtc tattttattt tccaaggaga accttcatgg
    60361 aaggcgttct tgcagcagga attaaaagat tacttaggaa aacgtgtctg cttcttacct
    60421 atcgaactaa ccgatctttc cactatttct tttgaaaaaa cagatatctt gatttccaat
    60481 ttccctctag attctctaga tatccctatt gtatacattt cttctattcc aacgaaaaat
    60541 gagctcaatc gtttgactga actcactttc aaaacttttt tatagtaacg gctagcgact
    60601 gtattctcta gctgtttttt cttttttgcg aaacagtttg ttcatgatcc ctttttctat
    60661 ttctacaact aagaatagaa gaatcacatt tgctccaatc aataactgtt gtgtcatcgt
    60721 taaacttact gtaccgatca gctgctggcc gactggtaga aaaataacca tcccttgcaa
    60781 taaagcaagg atacctaatg aaagaaaaag cgctttgttt tgcaaaagac cttggttcaa
    60841 tgacggcttg gacaactctc tgcaattgat catgtaaaca gcttgagcta agacgatatt
    60901 ttgcagaaga atggtttgct gtaagctttg cccttcaaac tgcatagcca ataaataggc
    60961 aggaaccatg atcagtaggg agacatagat gatccgaaac aaatgataac cagataggat
    61021 tccttgcttt ggatctcttg gtgggcgctt catgatttct ggatctggcg actcgaaccc
    61081 tagtgcataa gataaagtga ttgtcgttac catattgacc cataaaatct gtactgggga
    61141 caaaggtagc gggcgattca gcataagtgc ccaaatcaag atcaatcctt gagctaatgc
    61201 agttggcaag aagaaattga tggttttttg caaattatcc aaaatccgcc ggccctcttt
    61261 tacggctttt gcaatcgtat ggaaattatc atctcctaaa accatatcag ctgcctgttt
    61321 gcttacttca ctacctttga ttcccatagc aattcctaca tcggatcttt tcaaagcagg
    61381 tgcatcattt acgccgtctc cggtcattcc gacgatttct ccatttttct gcaaagcttt
    61441 aacaattcgc agtttatgtt ctggtgtcgt tcgagcaaag acatcgacta ttggcacttg
    61501 ctgaatcaac tcttcatcag acataagatc caaatcgatc ccttctaaaa ctttggcagt
    61561 atgttttaag ccaatttgtt ccgcaatcgc ttttgctgtg tctttgtgat cccctgtaat
    61621 cattttcact gagatccctg cttgttggca ttcctttaca gctttgactg cactttcttt
    61681 tggcggatcg atcaatccgg cgatccctgc aaaagttaat tgggttaaag tctcatgcgt
    61741 gagttcctgt ttcgaatcta cttttttata agcaaatcct aaaacacgct gaccttttct
    61801 cgcaaattct gcagctgtct gactccatgc ttcttgttct gcagaagaaa gagttgattt
    61861 agcaaataat acttctggtg cacctttgac gtaaatcacc gctccttttt cctccgcatg
    61921 catcgttgcc atatatttgt attcagaact gaacggaatc tttgcttcta tttttttcaa
    61981 aggtagattg gctgtttctg tgtgtcgaat cagcgcaact tctgtcggat tgccacttaa
    62041 attttctgtt ttctgttctt cttttgtttt taattcctga cagtttttca taatggacaa
    62101 cacgcaggct tcttcttttg cagctacatc cacaactgtc atttcatttt ttgtcaatgt
    62161 tcctgtctta tctgaacaga tcactgtcat agaacctaat gtttctaccg aaggaagtcc
    62221 tttgatgatc gccttttctt ttgccatttc atgtactcca acggatagaa tcatggtcaa
    62281 aacagctggc aatccttcgg gtaccattga caccaccaaa gcaatcgtag ccgaaagaag
    62341 gatatgccaa tccataccat atcgaaatgt cgtaaaaata actaagaata ggctcaaaca
    62401 aagaatgcct tgaaaaattt gtttgtttaa tcggttgatt tttttaatca gaggcgtcgt
    62461 ttgttgttcg accgattgca atgcttgatt gattttaccg atttctgtcg tgtttcctgt
    62521 ttctacaacg acaccgactg cagaaccaga ttgtacaagc gttcctgaaa aagccatatt
    62581 tttctggtcg ccgatcgatt cttcttctgt caaaacggca tgactttttt cagatgcttc
    62641 tgattctccc gtcaaaatcg attcttccac taataaatca tgtgcttcga ttaatcggat
    62701 atctgctgga acgacatctc cggcacgtaa gacaatgaca tctcctttta ccagttcttt
    62761 cgcagggatt ttttcttgca tcccgtttga tagtactatc gcttcttgtc ccatcagctg
    62821 cttcaaacca ttcaatgatt cttccgcttt tctttcttgc cagtaactaa cgagtccatt
    62881 gacgatcact acgaataaaa taatacttcc ttcaatatat tcactagaag caaatttcaa
    62941 aaaagaggca atgatcaaca cgatcatcag cagatcagta aaatgctttg ctattttttt
    63001 ccatcgtttt gtctgctgcc ttgcttttat ttcattatat ccttgatcag tcaggcgttt
    63061 ctgtcgttct tcttgcgaca agccttctgt tgatgtttga tagtttttca atacatcttc
    63121 taccgtcaat ctgtaccagt tcatcctatc ccctcctgtt taattgatac tgttattata
    63181 cgcagggaag ttttttcagg cgtatcgatt atttcagggg ggtctgaaat aattcgtttt
    63241 gcaaaatgta tctgggccta actgatattt ctattgctta ttcttttata tacaaaattt
    63301 aagaatacaa accagataac caataaaaaa ggaataaagc gtgtcgtgct ttcgaataac
    63361 aagctgaaag caattgaagt aaaaaacacc aacaaagcaa gatctttata tctttgtgta
    63421 gtgttccgtt tcttttcttt aataaattga acatgtgcac acaagatcga agaccacaca
    63481 aatagaaaac aactcgttgt catgcctgcc aaaagctgaa aaaacaatcg actatcagct
    63541 acaaaaaaat gtagacaaag accggcaaaa aataacattc ctgtaattaa aataccgtgt
    63601 aaaggtacgc ctttcttaga aagcgcacaa aaccaatggg gcaaactttt ttcttttgct
    63661 gtttgatata aaatcctgct agtactgtag actgcactat ttgctgagga caaagaagca
    63721 accaaaatca ttagattgac aattgctgct ccgccttggc aatggatcac ttgcagcata
    63781 gtcactaaag gactttcttt taaatctaaa caatgccatg gaacaacaaa taaaatcgac
    63841 aaaatcgttc ctatatagaa aaagagaata cgccaaggca gacagttgat tgcttttttc
    63901 aaattctttt gcggatcact tgtctcgcca acggttaagc cgatcatttc aattccagca
    63961 aaactgaata aaaccatagg aaatgagact accattccag caaagccaaa cggtagtaat
    64021 tcatcgatct gtccaaaatt ttctttgatt ccgatagact gggtatgaaa atctgagaca
    64081 ctcaatccta tacccaataa gataaaaagg aaaatcccca agatcttgat gattgagaaa
    64141 aaagattcca attcaccaaa ccacttgact gcaacaaggt tgactgccat taagatcatt
    64201 agtgcgctac ctccgcttat caaagctggt acatgtacaa accagaaatg gatatacaat
    64261 cctattgctg ttaattccgc aagtccagct ccaagccagc aaagccaata agtccaattg
    64321 acaaaaaatg cccacttttt gcctaaataa tagtcaacga aatcactgat agagttaaat
    64381 gtactatttg ctaaaaataa atcacccatg gcccgcatca aaccataaat agccaaaccg
    64441 catatcaaat aaacgagaat aacagctggc cctgctgatg caattgcctt tgctgaacct
    64501 aaaaataatc ctgttccaat tgcgccacct aatgcgatta actggatatt tcgattagtt
    64561 agagtccgtt tcattgccgg tttttccatc aagttcacct ttttcttttt caagcagcat
    64621 ggcgacttcc tgttcccaag aaggtttctt tattttttca atacagagtg gtactacaaa
    64681 caaaatgaca ctgatggctg tgactgcaat tagattgatc catgttccag ctttcatcgt
    64741 cagtccgaca gaaaatccga ttgccgcaaa taaaacaata acgacagtcc aaactccaag
    64801 attgctcttt ttctcttttt ttccaattct aaatggtcga ttcaactttg catgtgtata
    64861 acgcaatctc aaaactgcga tccccatgat gacatagacg atacagtaga taacagtagt
    64921 tgaatttacg agcatcaaaa atgcttgatt gacgccagga atcagcaaat aaaaaagtgc
    64981 aaagattgag ataatgacag actgagtcaa aataacggat ttggatacgt caaattgatt
    65041 cgtcttccaa aaattgaatt ttggtggata cgcacctttt ctagcacttt ctgtcactgt
    65101 ctttgctggt cctgaggccc aagcagataa ttggactgct acaccaatga agacaagtaa
    65161 actaaacaga ttcacaataa gatgtggcaa accaagaatt tggcaataaa tcgaaataga
    65221 ttgcgcgata ttattcaatt ccatttgtcc ttttggtacg acatttgcta caactaaagc
    65281 attcaaaata ttgacgaaaa atataaaaat taacgcggca aatactccgc gaatatacgt
    65341 tttaactgga ttttccaatc gagtgatata cacagaggac atttcgatac cagtaaaaat
    65401 aaacatgatc ggcgcaaaat aaacaaatga ggatgcagtc gtagtatctg gtattaattt
    65461 gtcccatgaa aaactgccta atgttccggt tggtacgatc ccaactttga tccacgcagc
    65521 aaaccctaat aagagcatca tcgtcaaagg cacgtacaat cctaaccaaa caccgacttt
    65581 tccgccgatt tttgccatat caaatttcaa attcaaaaga gtgatgatcc aataaacgac
    65641 taaaatgaca actaatgtaa atttgctgtt caaaccaagc ggcacattat cgataatatt
    65701 accaaacaac ggagcgagtg ctgaagcgac catcaccatc ccaggaaaca tttggaccca
    65761 taacagccat gagacaacga acccccattt ttttcctaga gcattcgata cccaaagttc
    65821 tggaccacct ttttggggaa acattccagc aaattcacca gagatcaatg agattggaaa
    65881 agcgtacaat aatactgcaa ccagcatgta aaaaatcatc gtccagctcg ttgctgctac
    65941 atctggaata tttcgtacac ttgctacaag ggcgcaggtc attccgataa aggtcatggt
    66001 cgtgatttgc tttgatgaac cagtatcttt catctttatt ccttctttca atagcgacat
    66061 atgtttttgt tttcttttat ttttgttcaa gaacagaaat gacaaggttt catcgattca
    66121 gtacaaaacc cagtatcacc gaaacatcca ttgaacgatc ttagtgaact gggtttgata
    66181 catgaaacga acgatccaac ttatagaacg ttttataaaa aagaatatct tggcgaggaa
    66241 tatatgaaaa acagggggaa atacaattgt tttgatgaag caaataattg tctgttctcc
    66301 atatgtctct tgttattctt tcacacaaaa atcagttcta ttggatgatt tcgtttagtt
    66361 tcttttccat tgctttggcg attttttttg tataaaaatt gaaaattcga tcatcattca
    66421 aataaggagt catgatagcc gcacgtagca gagtgatttt ctgttctttt ttccattctt
    66481 ctttcgttag acccattctt tcaacaaaac gaatcggaga atcaccgtaa tcttcttgtg
    66541 taaatgtagt atgagaagta ataaaccgtt caccataaac atcgccatcc atataagaag
    66601 accgatcgaa cattttttca tttaattcat tgatagcgtt caaatctgta cagccttgtt
    66661 ctttgaatac ccaattgacc atattaaaat caggatgatt caatggatag acttcgattg
    66721 tttttccttt aacagtaaaa gtcagatgat cgagaaattc tctgaatctt tgagctgcct
    66781 cgattgaagc cccaatcaat tgaccatatc cggtcacatt caatggcaat acacgatgtg
    66841 ctgtccagac tgcggcggcg gttgctccag cttttgatcc ttccaaaata taagctccta
    66901 acatatctgg agcctttact gatttttcaa acacatatgg cgcgaaataa gaaatgatat
    66961 ttctcatatt tttatgtttg attacgattc ctccagcagc ataagggaca tagcccatct
    67021 tatgaggatc tatcgtgaca gaatccgctt ctgaaatagc tctgaaccct tcatatacct
    67081 ctttgtcaat cgttacgcag tggtggaaaa catgatgttc ttcaaagaat tcagctaagg
    67141 aagcatacgg cacaaattct cctgcttcgt ttagaaacag tgaacgcgca tagccgccat
    67201 atgcggcatc aacatgtaaa tagaaataga tcccttcatc ttttaacctt tctcttaatt
    67261 ggacgatttt atctacactg tctacttgac cttcttctgt cgttcctacg acagcaacca
    67321 cacctagaat tgggattttt tgtccagcta attcacgaat tgttttttcc aaagcattaa
    67381 tatccattcg ataatcctct tgaactggga tagcaaccat ctgatctaag ccgactccgc
    67441 aaatatctaa cgccttcatc caagaatagt gttttgtttg agggaccagc catttcccta
    67501 atctttggat attttttccg ctacgggaag aagctgcttt gacctcatcc atttcttcgt
    67561 ccgtaaagct ttcggtcatt tccaaaattt cttcaacaga taaattcaaa agttcccact
    67621 cagacatttt tttcactttt tcaggcagca cttccttgac agctaaagga atcgatttga
    67681 tgcaacgggc gtaccataat ccttcaagat tagcaataga accatctgct gcaatgtgtc
    67741 cccatccgtc tgtcatattg aataatgaag caaaatcctg tcctacttct gcttccattt
    67801 gcgaagttgc catagaagat tccaatgcaa cattatttgg gttccacagc atcgcatagt
    67861 tgtaagctaa taaagcaggc atcaatgttt cagcattcat ttgtccccag taacggcctg
    67921 ctgaatgcca tgggatcgat cctgcgcgta atcgctgact gacttcatcc aagacctcta
    67981 tcatatggtc gcgggtggct agataatctg gagtgagctt atctccttcg ctaattgcag
    68041 gcatatctga aggaatataa ttttctctcc atccaagatg ttcatctacc atcttgttca
    68101 acaacatttt ataaactggt ccattttcag ctttgtcgcc tataaagacg gccttgatat
    68161 ccatatcttt catttttctt ccctccatat tttgttggtc aatgtcttgc agatacatgt
    68221 gtttatctgt aacacctctt cactatagcg ccaaaaatga gaaatttttt tttataacat
    68281 caaatccacg tagattttta caaacaaaaa taaataaagt cggatttttc tgataattca
    68341 acgcaaaaaa gcgaacaatt tttcaataaa tcagtcttct aaaatagata acctaaaaaa
    68401 agcgaacaaa caaataaaat gactgtcctt ca
//
LOCUS       DAJSBL010000009        63475 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.009, whole genome shotgun sequence.
ACCESSION   DAJSBL010000009 DAJSBL010000000
VERSION     DAJSBL010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 63475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 63475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..63475
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.009"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(152..550)
                     /locus_tag="ONT85_000698"
     CDS             complement(152..550)
                     /locus_tag="ONT85_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HCR2377693.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(547..1320)
                     /locus_tag="ONT85_000699"
     CDS             complement(547..1320)
                     /locus_tag="ONT85_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HCR2377694.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(1317..2000)
                     /locus_tag="ONT85_000700"
     CDS             complement(1317..2000)
                     /locus_tag="ONT85_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HCR2377695.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(2167..3054)
                     /locus_tag="ONT85_000701"
     CDS             complement(2167..3054)
                     /locus_tag="ONT85_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCR2377696.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(3051..3920)
                     /locus_tag="ONT85_000702"
     CDS             complement(3051..3920)
                     /locus_tag="ONT85_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCR2377697.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(4057..5343)
                     /locus_tag="ONT85_000703"
     CDS             complement(4057..5343)
                     /locus_tag="ONT85_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR2377698.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            5616..6194
                     /locus_tag="ONT85_000704"
     CDS             5616..6194
                     /locus_tag="ONT85_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HCR2377699.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            6194..7123
                     /locus_tag="ONT85_000705"
     CDS             6194..7123
                     /locus_tag="ONT85_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR2377700.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            7120..8856
                     /locus_tag="ONT85_000706"
     CDS             7120..8856
                     /locus_tag="ONT85_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HCR2377701.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(8919..9755)
                     /gene="pheA"
                     /locus_tag="ONT85_000707"
     CDS             complement(8919..9755)
                     /gene="pheA"
                     /locus_tag="ONT85_000707"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HCR2377702.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(9752..10261)
                     /locus_tag="ONT85_000708"
     CDS             complement(9752..10261)
                     /locus_tag="ONT85_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HCR2377703.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(10254..11549)
                     /gene="aroA"
                     /locus_tag="ONT85_000709"
     CDS             complement(10254..11549)
                     /gene="aroA"
                     /locus_tag="ONT85_000709"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HCR2377704.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(11562..12653)
                     /locus_tag="ONT85_000710"
     CDS             complement(11562..12653)
                     /locus_tag="ONT85_000710"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HCR2377705.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(12670..13836)
                     /gene="aroC"
                     /locus_tag="ONT85_000711"
     CDS             complement(12670..13836)
                     /gene="aroC"
                     /locus_tag="ONT85_000711"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HCR2377706.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(13838..14902)
                     /gene="aroB"
                     /locus_tag="ONT85_000712"
     CDS             complement(13838..14902)
                     /gene="aroB"
                     /locus_tag="ONT85_000712"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HCR2377707.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(14935..15957)
                     /gene="aroF"
                     /locus_tag="ONT85_000713"
     CDS             complement(14935..15957)
                     /gene="aroF"
                     /locus_tag="ONT85_000713"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HCR2377708.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
     gene            complement(16616..17590)
                     /locus_tag="ONT85_000714"
     CDS             complement(16616..17590)
                     /locus_tag="ONT85_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HCR2377709.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            complement(17571..19454)
                     /locus_tag="ONT85_000715"
     CDS             complement(17571..19454)
                     /locus_tag="ONT85_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HCR2377710.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            complement(19472..21697)
                     /locus_tag="ONT85_000716"
     CDS             complement(19472..21697)
                     /locus_tag="ONT85_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HCR2377711.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            21812..22846
                     /locus_tag="ONT85_000717"
     CDS             21812..22846
                     /locus_tag="ONT85_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR2377712.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            complement(22913..23926)
                     /locus_tag="ONT85_000718"
     CDS             complement(22913..23926)
                     /locus_tag="ONT85_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR2377713.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(24004..24192)
                     /locus_tag="ONT85_000719"
     CDS             complement(24004..24192)
                     /locus_tag="ONT85_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HCR2377714.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
     gene            complement(24632..24760)
                     /locus_tag="ONT85_000720"
     CDS             complement(24632..24760)
                     /locus_tag="ONT85_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HCR2377715.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            complement(24763..26136)
                     /gene="rlmD"
                     /locus_tag="ONT85_000721"
     CDS             complement(24763..26136)
                     /gene="rlmD"
                     /locus_tag="ONT85_000721"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HCR2377716.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            complement(26247..27302)
                     /locus_tag="ONT85_000722"
     CDS             complement(26247..27302)
                     /locus_tag="ONT85_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HCR2377717.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            complement(27312..28742)
                     /gene="gatB"
                     /locus_tag="ONT85_000723"
     CDS             complement(27312..28742)
                     /gene="gatB"
                     /locus_tag="ONT85_000723"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HCR2377718.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            complement(28739..30211)
                     /gene="gatA"
                     /locus_tag="ONT85_000724"
     CDS             complement(28739..30211)
                     /gene="gatA"
                     /locus_tag="ONT85_000724"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HCR2377719.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKTTFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            complement(30211..30516)
                     /gene="gatC"
                     /locus_tag="ONT85_000725"
     CDS             complement(30211..30516)
                     /gene="gatC"
                     /locus_tag="ONT85_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HCR2377720.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            complement(30622..32658)
                     /gene="ligA"
                     /locus_tag="ONT85_000726"
     CDS             complement(30622..32658)
                     /gene="ligA"
                     /locus_tag="ONT85_000726"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HCR2377721.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTIKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            complement(32675..34909)
                     /gene="pcrA"
                     /locus_tag="ONT85_000727"
     CDS             complement(32675..34909)
                     /gene="pcrA"
                     /locus_tag="ONT85_000727"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HCR2377722.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLVNPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            35064..36623
                     /locus_tag="ONT85_000728"
     CDS             35064..36623
                     /locus_tag="ONT85_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HCR2377723.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQNVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            complement(36734..38665)
                     /locus_tag="ONT85_000729"
     CDS             complement(36734..38665)
                     /locus_tag="ONT85_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCR2377724.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAVAEA"
     gene            complement(38683..39597)
                     /gene="pfkB"
                     /locus_tag="ONT85_000730"
     CDS             complement(38683..39597)
                     /gene="pfkB"
                     /locus_tag="ONT85_000730"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HCR2377725.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(39594..40346)
                     /locus_tag="ONT85_000731"
     CDS             complement(39594..40346)
                     /locus_tag="ONT85_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR2377726.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLLNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            complement(40505..40714)
                     /locus_tag="ONT85_000732"
     CDS             complement(40505..40714)
                     /locus_tag="ONT85_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2377727.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(40725..42053)
                     /locus_tag="ONT85_000733"
     CDS             complement(40725..42053)
                     /locus_tag="ONT85_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2377728.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            42189..42344
                     /locus_tag="ONT85_000734"
     CDS             42189..42344
                     /locus_tag="ONT85_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2377729.1"
                     /translation="MGLKFERTDNLDKLFESFAVDPEKTVAPKEEIDRFLKPQPQNEK
                     KKDKKQK"
     gene            complement(42418..43005)
                     /locus_tag="ONT85_000735"
     CDS             complement(42418..43005)
                     /locus_tag="ONT85_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HCR2377730.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(43088..44338)
                     /gene="clpX"
                     /locus_tag="ONT85_000736"
     CDS             complement(43088..44338)
                     /gene="clpX"
                     /locus_tag="ONT85_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HCR2377731.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVFNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVELAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            complement(44585..45874)
                     /locus_tag="ONT85_000737"
     CDS             complement(44585..45874)
                     /locus_tag="ONT85_000737"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HCR2377732.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            complement(46028..46960)
                     /locus_tag="ONT85_000738"
     CDS             complement(46028..46960)
                     /locus_tag="ONT85_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2377733.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLKEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            complement(47117..47776)
                     /locus_tag="ONT85_000739"
     CDS             complement(47117..47776)
                     /locus_tag="ONT85_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HCR2377734.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSLYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAVFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            complement(47779..48804)
                     /gene="sppA"
                     /locus_tag="ONT85_000740"
     CDS             complement(47779..48804)
                     /gene="sppA"
                     /locus_tag="ONT85_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HCR2377735.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELAPNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAIR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            complement(48817..49107)
                     /locus_tag="ONT85_000741"
     CDS             complement(48817..49107)
                     /locus_tag="ONT85_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2377736.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAQAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            49516..51321
                     /gene="glmS"
                     /locus_tag="ONT85_000742"
     CDS             49516..51321
                     /gene="glmS"
                     /locus_tag="ONT85_000742"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HCR2377737.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDASDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            complement(51393..52748)
                     /locus_tag="ONT85_000743"
     CDS             complement(51393..52748)
                     /locus_tag="ONT85_000743"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HCR2377738.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(52772..53941)
                     /locus_tag="ONT85_000744"
     CDS             complement(52772..53941)
                     /locus_tag="ONT85_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2377739.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            complement(53938..54825)
                     /locus_tag="ONT85_000745"
     CDS             complement(53938..54825)
                     /locus_tag="ONT85_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HCR2377740.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            complement(55014..58706)
                     /gene="nifJ"
                     /locus_tag="ONT85_000746"
     CDS             complement(55014..58706)
                     /gene="nifJ"
                     /locus_tag="ONT85_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HCR2377741.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIFPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPTMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLDNANPN
                     LAKALFEKTVHDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARRAARAERRKKMEQATKEKKEK"
     gene            58905..59426
                     /locus_tag="ONT85_000747"
     CDS             58905..59426
                     /locus_tag="ONT85_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HCR2377742.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLACPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            complement(60125..60682)
                     /locus_tag="ONT85_000748"
     CDS             complement(60125..60682)
                     /locus_tag="ONT85_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2377743.1"
                     /translation="MRNLSNRNKILIIIVVIAVFHLGTNAVLSRIILGPKPPRPEITR
                     GEFDFRLEYEVDGERIVIEDTIVALFDGFSADAGSMAWYRTWRLHLASDRRSRNILLD
                     ELEDGRRIYYVPESANYSMGDVQKKREPNPNWYPFNGVTIEYPRNKTPEIGAKFISGL
                     EDLYDRFGIRLISWEHDPPIENRFE"
     gene            complement(60861..61472)
                     /locus_tag="ONT85_000749"
     CDS             complement(60861..61472)
                     /locus_tag="ONT85_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2377744.1"
                     /translation="MNSNRGNKMEYNGSMRIVQIIFWITSGIVIIGGVFLMLPSLIFP
                     FFALISPKIPEPEITYGEFPFRIEYELDGQINIIEDTVIAEFNGCEFSAGSMKRERRW
                     RSRLASGREDLPFGDDLGIYFSRGSAQYYMGENVQSMSLKPHIALRNLEEGFKREVDF
                     ILGESGYIRTVLNFGEAQEVLTQYGITLINWEISEPIVNNFGD"
     gene            complement(61585..61842)
                     /locus_tag="ONT85_000750"
     CDS             complement(61585..61842)
                     /locus_tag="ONT85_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2377745.1"
                     /translation="MRAVHVNPTIEITMIGHSKGGAEAANSVAINTNAIIFNPATVNL
                     SAYELDSNTYTANMVAYVVEGDALNVVFGGVYTYWRINHVR"
     gene            complement(62439..62717)
                     /locus_tag="ONT85_000751"
     CDS             complement(62439..62717)
                     /locus_tag="ONT85_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2377746.1"
                     /translation="MEKHNITGSMYVDDQWFISTDEVKIAAIRRDYLQKIHKIKDPNG
                     KKARLIITLITSNGKKLKLAGSYDNVKKFEHWYLQKDSISLNKQFEKN"
     gene            complement(62751..63002)
                     /locus_tag="ONT85_000752"
     CDS             complement(62751..63002)
                     /locus_tag="ONT85_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2377747.1"
                     /translation="MKEKPINKWSLYKNSMFGVVIFGVCAGLFLFILLQTVTLFSSNP
                     TYGWRDSIALGMFAGGYGFLLLFSILTLSPIHGLIQLWK"
BASE COUNT        17688 a        13738 c        11220 g        20829 t
ORIGIN      
        1 gcatattttg aaaagaaagt ctgatgctta gagtagaatt tcataaaaat ttactatttc
       61 ataagtattc acgagaaaaa tacaaacaaa aaagcggaaa taacgctcat ccgccttctt
      121 gtttgtgttt atttcctctt caaacgcttg tctatgattc agacttttct ctatcgttat
      181 tttcatctaa aagttcttgc attccgataa tttctcgata ttgatgaatc ttagtgatac
      241 caatgacttt gtctacatca atactaaaac aaataccatt acctggaata tcgatgttga
      301 tgccttgacc aatcgcatcc attactttat tcgtcaaact atcgggtact aatgtcaaca
      361 caatatcttt ttctggcgaa atgtccaaac ctaaaaattt ccctgaatca tgtacaccag
      421 aacctcttcc atgtaagatc gttccgcctg ctgcacctgc ttcttttgaa aagtcgataa
      481 catcttcgcc cattcctccg tcaacgatcg tcacaatcat gtgaagattc aatgctacaa
      541 gatctttcat ttgctttccc ccaatttttg caatctttct ttttcgattc tgtctttttc
      601 atcttttcgt ttataaatat atcctagtac taaagtggat aatattggtg tcaatgcaat
      661 cattgcaacc acaccaaacc cgtcaatgat cggatctctt ccttcaattt gtgcagcgat
      721 tgccacgctc attgataaaa taaatgtcga acacatcggg cccgttgcta ctccaccgtt
      781 atcaaaagcc attgctaaaa agatcttgtc cacttttcgt cctaaaataa atgctagccc
      841 gtatcctggg atcaagaaat aataaagaga gaatccgacg agcaaacgcc acatagacaa
      901 tcctactgct gcgccgatcc caattgaaat taccatcagc atgattttcg atcggattct
      961 cccctcgctc aattcttcaa cctgtttgat cattacatga atcgccggct cagcaaaccc
     1021 tactaagaac cccattaaaa agccaagggg aaccagtata taattgtaag gcaatcccgc
     1081 aatagcactg cctaaatgct gtcctaccgg tacataagcc atattgactc cgtgcaggaa
     1141 aaggatcaac ccaatagtcg ttatcaagaa acctttcata atgtcaaaga aacgcttttt
     1201 gcgcatacga aaactaatga tattcattaa aacaaatagg attacgatag gtgccactgc
     1261 caaaatgact tccatggtaa cgatttcgat tccatctatc acttgctgcc agaaactcat
     1321 tggaagatca cccccaaaat catcacggca agaatcggcc ctaacgaagc aataccgacc
     1381 attccaaaac tgtcattttc atttttcttt tgtttgatca tactagtcat ccctccggca
     1441 agggccaaga taaatggaac agttactgga ccagtagtca ctccccccga atcaaaagca
     1501 atcggcataa attcggaact ggtgaaaaat gaggcgatca aaacgcctat gtagcctata
     1561 gccatcaatt gataatagga tagcttgaat acgacccgaa gcagcgcaaa ggctagaaat
     1621 actccagtgc cgacgctgac gatcccaatc agcaacactc tgccaatctc tccgtcagat
     1681 atatcatata cttgttgtcc caaaacttgc acggaaggtt cagcaatcgt gatcacgatc
     1741 ccgatagcaa atcccaaact gaccaaaacg gtcaaacttt tctttttgat catatatttc
     1801 cctactagat ctccgacttc catcatagaa taatctgctc caaacaaaaa gagcgtcatt
     1861 ccgatcatca tgattgccgc accaaccaga aatgaagcga ggtcttctcc ttctaacggt
     1921 gcaaaaacaa acgttaaaat gacaatcagg acagtcatcg gcaatatagc agcaatcact
     1981 tctttaaaat tttttagcat gtatcctcct cctttactct ttaagtttac cttaaaatga
     2041 aagagttttc gagcacaaac ttaaaacgtt atcatttaat ttttctcaac ataaaataac
     2101 acacgtccca aaagtatctt tttacttgga aaaatgactt tgaaggaacg cgcttattgt
     2161 gtttttttat tttacagagc cgatcattcc ttgaacgaat tgcttttgca aaacaaaaaa
     2221 tacgatggca gttggtaaag tagcgatgac taatccagcc atgatcattc catagtctgg
     2281 tgtataggaa gcacccatag cagataaaac aagcggaacc gtccgtttat caggagattg
     2341 caaagctact aagggccata aataattgtt ccagctactc atgaatgtga tgattgcagc
     2401 cgcggcatag gtatttttag ccgtcggcat atacactcta aaaaaaatac ttagttcttt
     2461 tagaccgtct aatcgagcgg cttctactag atcttttgga aaagatttaa cattttgtcg
     2521 aaagaagaag atcaaaaaag cagtgctaac agatggcagg atgacaacca agaaactgtt
     2581 gattcccaaa accgttccgt tcaattgtga aaacattcga tataaaggga tcatcaatgc
     2641 cgcaaaagga accatcatcg atagcagtag aatattgaaa attaggtctt ttcgtttact
     2701 ttgataaatc tcaaatccat atcctgccat tgacgaaatc agcaatgcaa acacagtggt
     2761 caccaaagcg atcatcgctg aattccaaag ggaacgagta aaattcagct cattggaaaa
     2821 caaattttga aaattgatga tcaagttact gccaatcttt atttttcccg atgtgatatc
     2881 cacgggtgta ttcgtcattc ccaaaaccat ccagacaaaa gggaaaatgg aaataaatga
     2941 taggatgctt aatgctgtat actttaatac tgacccaatt gtcgaacgaa gttctaatgc
     3001 ttgttttttg acgttggggc gttcgattgt ttctttttct accatatttt tcatcgactt
     3061 tctcctccta ctttcgtttg gattatcgac aatatcacaa taaaggaaac aataacaaag
     3121 gaaactgctg ccgcatatcc aaaattaggc gtgaatttat aactcaaatt atagatgtat
     3181 tgagataggg tggtcgttgc atttgctggt cccccgccag taatattttg tacttcatca
     3241 aaaagctgca atgtcccgat cgtcgaagta atggaagtaa acaaaatgat tggttttaaa
     3301 tttgggatcg tcactttgat aaactgctgc catttagaag caccgtcaat agatgctgct
     3361 tcataaatct ccgaatcaat attttgcatg cctgaaagga aaaaaatcat attgtatcca
     3421 gtccaacgcc aagtgatcga aagaatgatc agaactctcg cccaaattgg atgagtcaac
     3481 cagccaatcg gtgtgtcaat cataccaatg cccaacaaca agttgtttac aaggccagat
     3541 tgagaaaata gacttttcat gatcagagaa taactgacca tcgaagtgat acatggaaga
     3601 aagatagcgg ttcgaaacaa ccctttatat ttcaactttt tatcatttaa aaggttggaa
     3661 ataaaaagtg ctagaaacaa catgatcggt acttgtacaa gtaaatacag gaatgtatta
     3721 aaaaaagctt ttttaaaagt tgcatctgtc aacatccgct gataattacc taacccgtta
     3781 aatgtcatat tgacaggggg accactttga aaagacgtga tcaaagccaa aaccatcgga
     3841 ataaaaacca tgattgatat caaaatgact ggcaagatta aaaaagcatg cccgtttttg
     3901 aataaatctt tctgcttcat tcttttccct ccaattctat cattgattat ctacatgaaa
     3961 agacaagaaa gacaatatct gtaaggcaga cgtgatcttt tttgtctttt cgtttgtctg
     4021 atgaaaaagc gaagaaattc acttttttac caccgatcaa ttagccaatt gtgcttctac
     4081 ttgtttttgt gtatctgcta gtaccttgtc tgtctcttct ccattgatga ttcgatgaag
     4141 agcttcagct acgactgctt caattgcata ggtctcatgt ccgtaattca cttctggtat
     4201 ttcagatgtc catgttgaaa aatcgtcaaa gatcttttga ccactgtaaa attcagaagc
     4261 ttcttgataa gcttcttgtt cattcgcgct aagcatcgta gatactagtc cgatttcttt
     4321 cgcaagagtg cccatcagtt cttcattcga agcaaacgtt gcttctagga aatctttcgc
     4381 tgaatcttct ccagctactc ctttgatcac ataccagcca gcgccgccaa ggttagaagc
     4441 ttgcgctttt tgcagatttt ctggaagtgc aggtattggt gcaatcttcc attttccaga
     4501 ttggttttct gctccttgga tcgttgaaga ataccaagcg ccagttgggc tagaagctac
     4561 tgctcctgat tgtatcgcag ttactccagc attccagtca gaggtttgtt cgactagatc
     4621 ttctttcaaa agagtggcaa acaattcaag accatacttt aaagatgcat tgtcttggat
     4681 cgtgactgtc tccccatctt cagatgtata ccattctcct gcctgttgca taatcatcct
     4741 tactcgacca aggtcagaag gattgacttc agcgattttc tttcccgtct tttctttgac
     4801 atctcgcgct acttgaatat actcgtccca cttcagattc tccatatcct cttctgtata
     4861 acctgcttct gccatcagat ccgtacgata gaagtttgct gttacgcccg aatcgaaagg
     4921 aacaccgtat attttatctc ctactttgtt taccgcaaat ttatacggtg caaaatgatc
     4981 ttcctttaca atagcagaca aattagcgaa tgcatctgga taagaagtca aatagccttg
     5041 tatcctataa tcttcaatca agacgatatt gggcaatcct tctgtattcc cgctcgctaa
     5101 agcggtgttc agtttctgaa cgatatcatc ctgagacatc gtaacaactt ctatctctgt
     5161 ttcttcgttc tcataaactt tttttgcttc attcactgct ttgatattga atgtctcatc
     5221 ccacgcccag acagtgactt tttcttcttt gtctgctgta ctactagcac ttccgcatcc
     5281 tgttaagaaa cctgatgctg ccgctaaaca aacgactcca gcaattaact ttctgatgtt
     5341 catttttatc cgctcctttt ccatccatta gttgtttacg tttgctacat tcatcatacc
     5401 aaaatttgaa aacggtttct ttattattca gatacttcaa aaatttctcc taaaataata
     5461 aaaaaataca aatcaactat gacaaaagga ataatcttag catctagacc aaagatcgaa
     5521 atggtgaggt ttctttaaaa taaaaatatc cttgcatttt ctcctagaaa taagtatttt
     5581 taaattacaa gactattctt aaggcgggta aaaaaatgcc aatacgcgta attcttatcg
     5641 atgatgaaca gacgatttta gatggaatga aacagttgat caattggtct gagcacgggt
     5701 ttcaattaat agggactttt tccagtccgc tgaaagcgct tgatttttct ttggtacatg
     5761 caatcgatat cgtggtcaca gatgtaatga tgcctgaatt aaatggaatc gaattatcgc
     5821 ggctattaaa acagcaacaa cctgaaacac agatccttat cctctctagc tatgatgaat
     5881 ttgatatggt taaagattct tttaaagaag gcgtcgctga ttatctttta aagcctcgat
     5941 tgagtcctga tttttttcta aattccttaa ctgccgtttc acaaaaaatc aaaaaaaagc
     6001 ccaacaaaag cccaatcacc aatcgttatg agcagatctg cgagaatctt tcacagcaga
     6061 ttgctggaca aactcgtcca ttgcttgacc ctcttctttt tgaacacacc ttgtttttct
     6121 gttatatacc gaaaaacggc cattcagttc aaaacaaggc tctaaacaaa aacagtcgat
     6181 cacccattca ttgatggata gcgacgattc gtttactatt tatcctgttt ctaccttttc
     6241 ggaagaatcc ggctatgtaa tcaatactga ttccaaagat acacttagga agtttgttca
     6301 aaagtttcgt caagaagttt ttccaggtga tttttttgtt gtttctgatt cttttgcaat
     6361 ccaagatatc aatgaaatat tccaagagat cagaaattgc accaaaggac aacgtttcta
     6421 tcataaggga aagaaccttg cttatcagca tgaacttctt ccgttattaa ccaaccacga
     6481 gcagcaagca agtagttatt taacaaaaat cgttcataaa gactacgctt tagctgtcaa
     6541 agaacttgag aatttcttca cgacgatatt ggaacagcct attgatccag cttatttgaa
     6601 acatgaggca atcaatcgtt tctatgcatt actcaacgct ttggctgacg agcatgttgg
     6661 gaatgaagaa ctttctttgc tcaagatcgc ccttccttct ttattagccg atgctgagta
     6721 tttcgaagat ttttctattc ttgtcaaaga aacacttgag aaactcaccc acttttctgc
     6781 aaatgcaaaa aaagacaatt tcgatatctt gactttgatc tacgagttcg tccaggaaaa
     6841 ttatgagaaa gaaatcaatt tacaactttt atccgaaaaa tatcattttt catatagcta
     6901 tctctcagcc atctttactg aaaaatttgg tatcaacttt tcaaaatatc taaaaaaagt
     6961 ccgtatcagt aaagccaagc tctttttgac ggaaacacgg cttactttga cggaaatctg
     7021 tgagaaagtc ggctataccg aactgggtta tttctccaga gtttttaaag aagagacagg
     7081 aatgacacct tctcaatacc gtaaaggaac ttttgtaaaa tgagaaaaaa aatcaaagag
     7141 catgaactct ttgccaaact tctgttcatt ttgttcatcg gtctttttct gcaagccatc
     7201 gtcatttctt tgtttattta tcatcgatca agggatgcct atatccagct gttcaatcaa
     7261 tcgaatgatg tggtactaaa aaagattcaa tctgaatttg aaagtttgaa tgatacaatc
     7321 gagaatacat tagccgcttt tgattctaat ccagctgtca aaagctattt ttccaatgat
     7381 ccagcccaac atatggaaca gcttcagcaa ctaagaacaa tccaaaaaat gaatgactct
     7441 ctttcaaaaa tccatccaat gatcgattac gatgtattga tctttggaga aaatggaaga
     7501 acttttgtcg gaaacgacat gctcactgcc gtatctgccg attctttttt tcaatctgcg
     7561 attgctcaga gagtgaatga acgtgctgct gatacacaaa tgctttttgc acaccacgga
     7621 ttgactcttc gcgataagaa agcacccagc gtcttctttg tcaaaaaact gaaaaataca
     7681 ttgaatcacg tttacggata tgctgtttta tcgatctcat ccaagcaact agcgaatctt
     7741 tttcaatctg tagtaaatcc tgagatatcg aagatctcct tcatgaacga agaacagacg
     7801 atcatcgcat caaatgaaga aaatacaatt gggagccgat caatgatttt caaaaaacta
     7861 acagtaggtg aaacaacgat ttcaaatgga cagcagttga cccgtcttcc tctgtaccgc
     7921 caggattgta cattactcag tcaagtcgat cttcattctt tatctaacca aatgggcgtg
     7981 atttttccga ttattttgta taatatcgtg acacttgctt ttgctggctc attggtattt
     8041 atgtatttgg atcgacacac aaaatcgatc taccgcttga ttgattcgct aaaacggcta
     8101 gaggaagatg gcttaaaagc tactgttcct gtacaaggaa gccatgaagt ccaattgcta
     8161 gggaatacga tcaatcaatt gctaagtaat gctcagcatc attatgaaca gacgatccaa
     8221 gacgaaaaga agaaacgttc gcttgaaatc caaactatgc aagcacagat acagccccat
     8281 tttatttata atacgctgac tagcttgaaa tttttgattt ggcagaaaaa aacagatgaa
     8341 gcagttcaag gaatcgacag tttcattact ttactaagaa gtacgattgg gaaaaaagaa
     8401 gaagtgattc cagtcaagga agaaatcaaa agcgtacaaa gttacatcga tcttttgagt
     8461 atgcgatatg gcgaaagaat ctcggctaaa ataatgattc ctgatgaact gcagtcatta
     8521 tctatgccga atatgatcct tcaaccaatt atcgaaaacg cctatcttca tgcttttcac
     8581 aataaaacgc aaggattcat cacggtgtac ggcaaaacaa aaaatcagct tcttttattc
     8641 gaagtgattg ataacggcga tggcttcgat actgaaaata ccaaacaaaa aaatgatcat
     8701 ttttcaggaa tcggcatcag ccatgtagat gagcggatac gcctaatgta tggcgatgca
     8761 tatggcttaa cgatccaatc agtgattggg acaggaacga ctgtaacgat ttgtctaccg
     8821 cttcttccta aaaatcaatc agaatatcaa aaataaagaa tagccaagcg ctgatttttt
     8881 tattttaagt taaataaatc aatcagtatt cttgactttc atctacgcgt ctcttctact
     8941 gtcagcactg gataacagcc caatatttga ctttctcctc cgagcatctt gatttcttgg
     9001 agcgcattct ctactaatgt cattggacgg tccaacaaca aatcgatcac gaaaaaatat
     9061 tctcccaagc tagttttcaa tggtcgggat tcaatcttac ttaagttgat ttcccgccag
     9121 ccaaaagctg ccagcatttt atgaagcatc cctggccgat tagcaggtag tgtcaagatg
     9181 acactcattt ttactggagc tgactgctgg ctggtcatct ttcggtcacc gacgatccaa
     9241 aaacgtgtct ggttcaactc attatcttgg atattctcag ctaagatctc caatccaaca
     9301 tgctgtgctg tttctaatga agcaatcgct gctgcgtctt ctttcggatg ttctgctaca
     9361 tacattgctg cagcagttgt tgattctgtt gctactaaag gaacattagg ataatgcgtt
     9421 tctaaaaact gttgtgattg tgctagtgct tgtggatgtg aaaggatttt tgtaatcttt
     9481 gttgctggat tgcctaaaag ctgctgcttg ataggaagaa cgatttcact tttaacttcg
     9541 acttctggat gtttggaaag aagatcgatt gtgtgatgaa cagctccttc caatgaattc
     9601 tctacaggaa cgacagccag atcgacttgt ttgcgaaaca aagcgcgcaa acaaacaggg
     9661 atggaagcat acgcagtcaa ttgttctgta ggaaatagct gacaagccgc ctgataagtg
     9721 aatgaacttt ctggccctag atagctgacg ttcatatgct tttgatcctt tctataatgg
     9781 tatcgatgat cttttctggt gtttgattgg tcgtgttgat tcgatgtgtc gcactttctt
     9841 cataccaaga aagtcgttgt gccaataatg cttttatttc ttctcttgtt tttccaagag
     9901 ctaaaggtcg aatagacgtt tcatcttgtt ggatacgatc aactagacag tccgcttctg
     9961 cctctaagta aagaacaatc ggctgtgttt ttaaaaaacg ccgattttct gcactttgga
    10021 caatccctcc accagtcgcg ataattttgt cttcatttga cagctttctt aaaatatctg
    10081 actccacttt tcgaaagctt ttttcaccgt aatagtgaaa atattcggca atcgacaatt
    10141 ggatatgttc ctcaatcttt gtatctaaat ctacatactc tttttgtaaa cattgagcta
    10201 accctttacc gattgttgtt tttccagcgc ccatgaaacc aatcagcaaa atgctagcca
    10261 tgagcttctc ctcctgacac aagttctgct aaatcgtcaa aaaatgccgg ataagagatt
    10321 gaaacagctt cagctccttc tagttcgcaa ggtccatcag ctatcaaagc agctacttgc
    10381 agcatcatac cgattcgatg atcgcctctg ctggacactt ttgcgccatg cagaggtgtc
    10441 ggcccatgga tgatcagacc gtctgctgtc gcttttatct ttgctcccat tttctgtaat
    10501 tcttctgcca ccgcatcaat tctgttggtt tcttttactt ttaattcttc tgcgtctttg
    10561 atgatcgtga ttccttctgc ttgagtagcc actaaagcta gaatgggcaa ttcatcaatc
    10621 agacgcggaa tgatttctcc accaatcgtt gctttcttca aatgactcgt cttgacactt
    10681 aaatcagccg attggttctg ttcattgtga ttcgtctggg taatcgctgc gcccatctcc
    10741 tccaacacat ctaagatacc agttcttgtt ggattgaccc cgacattttt taacaacagt
    10801 tggctttccg gaacaagaag tcctgctgct agaaagaaag cagctgatga aatatcacct
    10861 ggaacagtta tctgttgacc agttaatttt tgcggtccgg tcactatgat cgttttatct
    10921 tccactgtga tccttccgcc aaattgacga atcatttctt ccgtatggtt acgggaacgt
    10981 tctttttcaa cgattacgga tgtgccttct gcttgtaatg ccgcaaacag caaagcagat
    11041 ttgacttgcg cactggctac aggcatattg tatcggatag gtgataaaga gcgggttcct
    11101 gtcacagtga ttggtagata ctggtcattt tcttctcctt gcaagtcagc gcccatctcg
    11161 cgtaaaggtt ccatcactcg ccccattgga cgtttagaca acgaagcatc cccgaacaga
    11221 gtagtcgtaa aaggctgtcc agccaaaata cccatcaata aacgtatggt cgtaccggag
    11281 tttcccatgt caattgattc ggttggttgg acaaatgagt ggctaccgcg accatgaatc
    11341 ttgatcacct cttcctcttc ttcgatctcg acacctaatt gttggaaggc atgtaaagta
    11401 ctcagacaat cttctgcgcg caaaaaattt tgtatagtcg tcgttccttc agcaattgct
    11461 ccaaacatga tgctgcgatg ggaaattgat ttatctgctg gtatcctaac agtccctctt
    11521 aatccatgta tttgttgtaa taattgcatt tatccagcct cctatttgat ccagcaatga
    11581 taactggtat tagacataat gattcttttt gcttgtaaca aatcctgttg gtttttaaaa
    11641 ctgatttgca gtattcctaa aatatcttcc ctggtctctt ggatcttcaa attaatcaaa
    11701 gaaactcccg cttttccgat cagtccagtt acttctgcaa taacgccagg ttcatctggc
    11761 acatcgataa ataaatcaaa aaaggcagga atcgcgcctt gcttatggac aggcaactgg
    11821 tctcgacttt cttttgcccg attgaaaaaa gaaaagattt ccgattggtt ctcagtcatg
    11881 atccattctg ctatttgtgt catttcttgt tgccaatctg ccaatcgttc caataatatc
    11941 tgcttattgc tcaacaaaat atctgtccac atgacaggat ctgaggaagc gatcctagtg
    12001 atatctcgaa agcctcctgc tgccagttgt ttggctcgcg gatactcttg attgaattgt
    12061 tctgcctgat tgactaagcc agctgccaga atatgtggaa aatgacttag cattcctgtt
    12121 atacggtcat gttctcttgc tgtaagagtt acatattttg ctttggttcc gcaaaatagt
    12181 tcttgcagtt tttcaacatc ttttttcgtc ttttccaaag gtgtaaagat gtaataggca
    12241 ttttcaaata gtttagcatc tgctgcctgt atacctgatt tatgagaacc agccattgga
    12301 tgtccaccga caaaccgaag atttttctgc tcagcggatg tcacgacttg ctgcttcgta
    12361 ctgctgacat ccgtaatgat gacgtgatca gacaagttga ttctttcaag gacatcaaga
    12421 taagctaagg ttgtacggac gggaacggct aataagataa cgtctgcttg ttctgcacct
    12481 gtttcaaaat cacttgcaac gtaatcaacg atctgctgtt ctactgcttg ctcacgggtt
    12541 tttgactgac tatcccaacc aactactatc agttctggat tggtctgacg aatacaaagt
    12601 gccatagaag caccaatcag tccaagcccc acgataaaaa catgttgtgc catcttattt
    12661 gccccctttt tagtaatgtt tcaagtagtc acggtactcc ttcactgcag ctttcaactc
    12721 aacaagcgaa tcacttggga attttgacaa tagctctgtc gctaccacag tggctacaac
    12781 attttccgca acgacgctag ctgcaggaac tgctgtacta tccgaacgtt ctacacttgc
    12841 tttgtaaggt tcttttgtat cgatatcaac actttgaagt ggcttgtaca atgtaggaat
    12901 gggtttcatg accccgcgaa tgacaagtgg ttcgccattg gtcattccgc cttcgaagcc
    12961 gcctagatta ttggtacgtc ggaaatagcc tctttcttct tcccatagga tctcatccat
    13021 cacttctgag cctggtaatg aaccagaagt gaatccgacc ccaaattcta cgcctttaaa
    13081 ggcattgata ctaaccacag cttgtgcgat tttggcatct agcttcgtgt cccattgtac
    13141 gtagcttcct aatccagctg gaacgcctcc aacgagcact tctacaattc ctccgatcgt
    13201 atctccattt ttctttgtct gatcgatcaa atcttttatt ctttgctcaa ctgctggatc
    13261 aacaactcgt acttctgacg cttcagaaat ttctttgatt tcttgaatcg agatatttcc
    13321 aggtatttct gcttggattc caccaagaca aatcacgtgt cctgctacga tgatgtcaag
    13381 ttcttccaga attttcttgg caattgcgcc gattgctaca cgcatggtcg tctctcttgc
    13441 agaggaccgt tcaagcacat tccgaagatc tcgatgatgg tatttcatac cgcctaccaa
    13501 atccgcgtgt cctggacgcg gacgtgcaac tcgccgcagt gattggtctt tttcaggcac
    13561 ttctgctaca gacatgacag ttgtccaatt tttccaatct ttattttcta ccactaaagt
    13621 gacaggtgaa ccaattgttt tgccgtgacg gatgccagac gtgatccgaa cacgatcttt
    13681 ttcgatcttc atccttcctc ctctgccgta accagcttgt ctgcgagcga gttcgtgatt
    13741 gatttcttct tctgacagag gtaatcctgc tggtagtcct tcaataatgg ctgttaattc
    13801 tggtccatgg gattctcccg cagtcatata acgcatctta cagtcctcct aagtaatctt
    13861 tgaatgtctc tgtgggaaca ggaacgattt ttgcttgccc aatcttttct agcaagatga
    13921 tattcaattg gcttcctctt gctttcttat catgggttat cgcgtggaat aattcatctg
    13981 gatgacttag atcgtacgta atcggcagat ggaatttttg aagtatcctt tctaaagcag
    14041 ctgtcgatcc agttggagac aagccgattt tttctgcatg agaagtgatg gctaccattc
    14101 caatagctac tccttctcca tgactgacta ccccatatcc tgctgtattt tcaattgcat
    14161 ggccaattgt atgaccaaaa tttaaaataa gtcgatttcc ttggtcaaag acatcttctt
    14221 ccactacttt tcgtttgatt tccaacgtag cagtaatgat tggaacagca tgttccagaa
    14281 gatctttttc gtctttcagc tcgttcagct gtttccaaag cgttgcatct gctatagctg
    14341 ctgatttgat gatttcagcg attccttctc gtaaacgacg taccggcaaa gtttttagaa
    14401 cctcaggatc aatcaataca ccagctggct ggctaaatgt tccgacaagg tttttcgctt
    14461 tgtttgtatt gactgctgtc ttcccgccaa tactagaatc tacttgtgca agtagcgtag
    14521 tcggaatttg aacgaaatgg atcccacgca tgaacgtcga ggccacgaat gctgctagat
    14581 cacccaccat tcctccacca agtgcaagta tcccatcgct gcgggtaaaa tcattatctg
    14641 ctaaaaaatc gtataactca gctgcttgtt ctaaagattt actggtctca cccgccggta
    14701 cgattgaaag gactggttca aaattagctt gtctcaattg gaacatgatt ttttctgcat
    14761 atagtggagc aacgtttgta tcagtgatca cagctacttt ttgaggtctc caaatcgttt
    14821 gaagccatga tccagcctgt tctagtgcgc ctttttcaat atgaatatcg tatttttggt
    14881 tatcaacatg tacagtaagc attcgtttca cttctttctt ttaaaaagct ggaattaatc
    14941 gagtgtctga atcttttcca ttgcttcttt catgatcgca acttctttca tcatgcgtga
    15001 gtaagtcttt tcattcaacg attggggacc gtctgaccaa gcatttgcag gatcaggatg
    15061 gatctctacg atcatgccat ctgctccgct tgccacaccc gcacgagcca ttggtggtac
    15121 aagttcccaa attcctgtac catgacttgg atcaacaatc acagggaaat gagttaattt
    15181 tttcatcaat gggacagcac ttaaatcaaa cgtattacgt gtcgctgttt cgtaagtccg
    15241 aatcccacgt tcgataaaaa tgacaggaga tttgtcttcg actgcaatgt attctgcagc
    15301 attaagccat tcattgatcg taccggaaat tccacgtttc aatccaacag gtttacctgt
    15361 tttaccgaca gcagatagca gtttaaaatt ttgcatatta cgtgcgccaa tctgtaggat
    15421 atcgctgtac tcagccacca tatcgatatg cccttcatcc atcacttcag taatcacttt
    15481 cattccaaat tcatcagccg cttgacggat gtatttcaat ccttcttctt ctaaaccttg
    15541 gaaagcatat ggagaagtac gtggtttgaa ggcaccacct cgcagaattt ttgcgccccc
    15601 ggctttggcc attcgcgcac attctctgat ttggtctaat ccttcgatag aacaaggacc
    15661 agccatcgtc acgaaactgc cgtcaccaat ttttactccg tctacatcca cgactgtatt
    15721 gccaggatgg aattctcgag aagtcaattt gtatgtgttt aaaatacgca cagcattttc
    15781 tactccttca tagcgtgtaa atgctccttc ttgtaaactt cgcgtatcac cttttagtcc
    15841 aatcactact cgttctgttc cttgatcgat tgagactttg aatccttctt tttcgatatg
    15901 atcgacgact ttttttattt gttcttccgt tgcttctttt ttcataatta ctatcattgt
    15961 ttttcccgct ttcttttgtt tacttatcta tcttggctga ttttctctac tttttcaagg
    16021 tggttttaca ttaattccta ctaaatgacc gtgtcctgtt gttctcattt tcgctccttc
    16081 tttcctactt atgacccaga taatcaacaa aaaaagatgt ccctgtagga aattgcctct
    16141 acagtgaaca tcttcaacga gccttaaaaa gcgctgcaaa aatataaaat agccactata
    16201 gatgaatttc tctacgtggc tgtaagtcgt gtttaaaaat ctttagccat catagataca
    16261 aacctggttt gtttgtaccc acgaagtcat gaatacaaac ctatctaaaa tagctaaagt
    16321 aatacgtatt cagttttgta agttttctag atttcataaa aaatcgctcc aaactgatgt
    16381 aagaattgta cctagtatag acataaaaaa atcgactgtc aatcattttt atgaaacttt
    16441 ttttaatttt ctgatagtta actttgtttt tcagaaaaat aacaattgat tttattgaag
    16501 aaaatagtca tgaacaaaag tatctgagaa cttttttact ctcttgttca tgacatatag
    16561 aataaagcag ctttgtcttg taaactccgt ttttcaaata ataatctaaa gattgctact
    16621 gaataataaa gccgatttca tggtcctctt cgctgctgat tcggtaaggt gactcaacat
    16681 ccgctcccca gctattgata cccccgactc ctcttacttt tgcaaaaatc gtaagaaccg
    16741 ttcgtctttc tggcggtagt tcttctatat gtgttgcctg ttccagttct tctgctttga
    16801 atggtaaagc ggaaaaagca aatggcgtat cctgctgttc aaatcgcaac gaaaaatctt
    16861 cttgatcttt atccgtatta tccaacgttt gatttcttgt tagttctacc catttcgttt
    16921 ccatatgcat gccgcattct tggggaacaa ggtatgggga aacggggagt cctgccactt
    16981 tataaatacc ttcgacacca ccagccatcc gatccggata tgtttcccct gataggcctt
    17041 catactcaaa aaattttgct ttcgttggca taatgaacct taaacccaat gcgggtaatt
    17101 ccggtaatcc tttttgtcca taataatgca tattgacaag cattttaccc tctccagcga
    17161 tttggtagga aattgtcact tgggtttttg gtactgtgtt gatttccagt gtaaaaatga
    17221 tttcaaccgt ttcagcgtat tcattacttg aatattgatt atttactgga gcaatcggca
    17281 aatcgatctt tttattgtcc accagtactt ctatttcttt gacttttgga tacaatccgg
    17341 ctcctaacca cataccggat ttaaatccaa atccgctacc tctgtcatta tcggtcgttg
    17401 ctcgccaaaa aactggaagt gtttcccggt atagccattc ttttccgttt ttattgagcg
    17461 attccagtcc gcctcttgta taagaaaaaa tataatgcgt attccccgat ttgacaccta
    17521 atgtacagtc gccgaagacg attgcaactt tttccgtatc catatgactt ttatttactg
    17581 tatttttcat aataatatct tacctcctgc atggcgggtt tttcttgtcc atctgcaaaa
    17641 acgatcccat tccctgagaa ttcatagtca cttggtttat cgtcaaagtc accgccatag
    17701 cgcatcactt ctctgcctgt gattggatca gtcacttgga tcgcctgatc tttaaaatcc
    17761 catatatagc ctccttggta catttcataa cgatcaagca acgtcatgta agaatccatt
    17821 ccacctaacg aattccccat gtcatgcata tattcacata agatgaacgg tttttccgct
    17881 ttattttcaa ggtagtcaat gatctcttct ggtggcgcat acatgcagct ttttacatct
    17941 gaaatcgaat cttcgaatgc tggatttgga aaattccctt cataatggac aagtcgatcg
    18001 gggtctactt ttttgaagta atcgttcatt tgtcgaatgt tttcttccgc ataagattca
    18061 tttccaagag accaaaaaag aatacttgga tggtttttta atgtttcgaa attcgttttt
    18121 gctcgatcaa gaacagccgc ctgccactga ggtactgcac ctggtacgtt gtaagaaggc
    18181 tcaaatgcgc ccattttttg ccatgagcca tgggattcga ggttcgtttc cgccatcaca
    18241 tggattcctg cttggtcgca taaataatac caagtgatct ggttggggta atgagatgtc
    18301 cgtacgccgt tgatctttgc attttttatg atagccaaat ctttttccat atcttctata
    18361 gtgatggcgc gtcctttttt cggatgccac tcatgtcgat ttaccccgtt gataatcatc
    18421 cgttttccat tgaagtagat gattttatct ttgatttcga ttttacgaaa accgaaggaa
    18481 tagtcaatat actccgttac tcttttttct tcgtctatcg tctccaattc aagcttatat
    18541 aaattgggct gaaaatgccc ccacagtgcc acattttcaa atccatcgat gcgaatcgtc
    18601 atttgctctt ttaacggata ttgctgttga tagatttcct tcccatcagg atctagtatc
    18661 gctacattca gctgactgtt tttggctact tgctcaaaag acaaatctac ttcaagtttg
    18721 ccattttgac atttgtcatc taagcgaggt ttgatccaaa gatcttctaa atggttttgt
    18781 cctcgagcaa ctaagaagac atctctgaag atcccaaaaa atcggaaaaa gtcctgatct
    18841 tccaagaatg cagctgtact tcttttgtaa acctccacag ctagaatatt ttcttcttcc
    18901 aaataaggtg ttaaatcaaa ttcagacgga gtgaacgaat cttcggcata cccgataaaa
    18961 tgaccattca accaaataaa cattgcttgt tctacaccag caaaatgaat cgagatttgt
    19021 tttcccttta gaccttcttc taatcgaaaa gtcgtcaaat acgaaccaac cggattgtct
    19081 ttagcagctg aaaagatccc ttccgcctct ttctcagggc ttgtcgtgaa agggggacgg
    19141 cgaaatacat gtccctccca cggatacatc gtattgatgt aatggatctt gtcataattt
    19201 gctaattcaa tatgactagg aacagtgatt tcatcaaaag aacaggcatc aaaatctttt
    19261 ttataaaatg tttttggtcg taccgaagga tttggcgaat aacaaaaacg ccatgatcca
    19321 tttaaagact gtttaaggga ggatgtggtt tctacttctt cccttgttgc ataacaaaca
    19381 tgatcgctat gtgcatttga ttggttcact ctaaaaactt gtggatcatc taaccatttt
    19441 atatttgctt tcatactttt ttccttctct ctcataactt tttgaaatga agcagttctg
    19501 tttggaaatc accttgaaaa tcggggaagt aaagtccaac tgtcatcaat tcggcccccg
    19561 aaaatatccg cttcgaggtc tgttcctgat aaagtgcctc atcatctaac cccatcagat
    19621 atacttcatg gaacgctggt tgagctgaag ctagtatcct ccctaaaaac acaatagctt
    19681 catcttgttt aggagacaca aacatccatg cggcaatatt ttcttcaaaa ggactcgcca
    19741 aacgataata atggccatac tggatcaccg gacgaatcgt ttgataggat ataatttggt
    19801 cttttacttg ctttttttct tcaggagaca gctttgtcaa atccagttca taaccaaaaa
    19861 cagcgctcat agctgtttct cctcttgttt tcaacgaagt agatctgtcg gtctgatgat
    19921 ttggtacagc tgatacatga gctgtgatgg acgaaatcgg ataagcaagg cttgttccat
    19981 attggatttt catacgctcc actgcatccg tgttatcact tgtccatgac tgcggcatat
    20041 aataaataaa cccggcatca aaccgtccac ctccaccaga acagccttcc cataagattt
    20101 ccggataatc agttgtcaaa tgttcaagta gttcataaag cccgagcata taacgatgcg
    20161 ctacttctcc ttgtctctgt gggtcaaggg taatcgaata gacatctgaa agcgaacggt
    20221 tcatatccca tttgatgtag tcaagaggaa tcgtatctaa aatctttctc atttgtttct
    20281 caatcgtctg ccgaacatct aaccttgtaa aatccagtac atgttgtgat ctggaagcag
    20341 aaggcattct acctggctcc tgcataagaa aatcaggatg tgttcgatac agttcagaat
    20401 caaccgagat catttcaggt tcaaaccata aaccaaattt caatcctttg ctatgagcat
    20461 aatctgctat ttcacgtaat ccatttgtca acttgccttc atactcgaac cagtctccta
    20521 atgaagaatt gtccgcatct ctttttccaa accagccatc atctaatacg aacatttcga
    20581 tgcctagctc ctttgcttca tcaatgatcg ccttgatttt ttcactattg aagtcaaagt
    20641 aagtggcttc ccagttattg accagaatcg gccgttcagc aaattgatga cgacctcttg
    20701 cgattctttc acgtagcaac tcatggtgga tctgactcat tttgttcaat ccctgacttg
    20761 agtaagaaag gatcatttca ggtgtttgaa aagactggcc tgcattcaat tcccacgaaa
    20821 aacgatggct gttgatccca ccgacgactc tcaattgatt gatctgatct ttttcaagtt
    20881 cgaaagaatg gttgccggaa taaacaaatt gaagtccgat cgcttctcca gtattttctg
    20941 tcgtatgatg atgaactaaa gcaataaagt tattcatatg gtgactgctc gttcctcgac
    21001 ggctttcaaa tacttttgtt ccagataaaa cagattgccg tgaaatctgt ctttcattga
    21061 catgtgctcc tggcaaagca atgacttcat caaatcgcct agtatgggca aagtctagct
    21121 ggaaactagc cgctttttct agtttgattg tttctccggt ttcatttctt atctttacag
    21181 atcgtgtgat cactgctcta tcagtaaata tggtatagaa caaatcgaaa taaatcgctg
    21241 tttctctatc cttaagtgaa atcaccaacg tttgtgcctc tgacttgtcc atcacataag
    21301 tttgaggcaa gccttcgatc tctggcttcc catctgtaat ataatggctg tcatatctca
    21361 gatccagtaa gtctgatcca ttcttgcgct gaatgatcga tgcgggcgtt cgaaagtcca
    21421 tgctgccatg agaagagtat tcttgcggta gtgtatcttt cgacagagac cggtcaggat
    21481 ttaagggaac atttcctgaa aatccacgat ctcttcgggg ataattttta ttgtctttgt
    21541 actgcttgac tggtttgcca aaataaatat gcgccaatac attgctttct tctatcttta
    21601 tcagataaga aagaaatgta tttcgtaaat ggaagactcg gctcttttca tcaaataaga
    21661 tgaaattttt atctgttttt ctatgcgtaa tatccatcat ttcactccca acaaaacgga
    21721 aaccgtttac ttttttttat aattcctgta tactgtttac taaacaattt tgtcaataaa
    21781 aattttttat ttttttgagg agtgtgtaaa aatggttgga attagagatg tcgcacgaaa
    21841 ggcaaatgtc tctcctgcta cagtttcccg tgtgttgaat aacgatcaga cgatccatgt
    21901 ggctcagact actcggcaac ggatagtaga ggcagctgct gcgttggatt atgacatcag
    21961 tactcgtaaa tatatcaaaa agagaatgcc ttcgattggt gtgatctcta cgatcggcaa
    22021 agcgagcgaa gaacaggata tctattacaa acatttgcga gtagggatgg aagaagaagc
    22081 tcgacgtctg catttaggga tgaatcgaat ctataatcta tctgataatc ctaaacaatg
    22141 ggaagatcta gatcagttag gtgcggtgat cgttgttggg actgttacga aagaatcgat
    22201 taccgccttg ttaaaacaaa acatgcaaat agtcgtcgtg gataaccctg acatccagca
    22261 agaggtcgat atggtgtatg cagatcttga acggatgact aaaaccgtac tggaattgtt
    22321 cttagaaaat ggtcataaaa gaatagccta tatcggggga tataaagtcg atatggatga
    22381 attcggtggg aagcagattt ctataaatga aaagaggatg cgtgcctatc agcattttat
    22441 gatcgatcat cacttagaaa aatatatgtc ttatcatcta ggtggctgga cagaaaaaca
    22501 aggtgaggta cttgcgacta atctattaga aaatgaatcc cctcttccta ctgccattct
    22561 tgtcgggagc gatccaatgg caattggtgt ctatcgggca ttgaaacaaa aaaatatcca
    22621 aattggagaa gagattgttg tagcaagctt tgataatatc gagacaatcg caaacctctc
    22681 ccctggcttg accagtgttc aaatcaatgc gaaatcaatt gggcaagcag ctgttcgaat
    22741 ggcattggag cgaattgacg gtatacggga cgaaccaatc attttgactt atcctgcacg
    22801 attgattgtt agggaaagtt ttatcccaag aacaaaaagg tattgaaaga gaggttgtga
    22861 aaagccgctt cgtttcttca tactcaaaac gaaacggctt tttcacaacc tcttacttat
    22921 acttattcac aaaatccaat gtctgctggt aacaattttg cgattctttt agcgtgtaat
    22981 caaattggta ttcatgggta atctcttttt ttgtatctgt ataaaacaag gattgcacgg
    23041 gtacctttaa ttctgtcaac cgattttcta atgcttgacc ttgctcctgg aatgagtatg
    23101 catttccgtc agttatatac gttggcggaa attccttaga aacatggtca gccacactag
    23161 cttctttcaa ttgctcactc gttttccaat cttttgttcc aatcaacgac cacgcaacgg
    23221 ttttgacgaa aaacttcata aatcgatcat ccgaatgctg ctgtgctgtt agcttcaaat
    23281 caacaggtcc gcaataagaa atggttcctt tcagactatc ttttggaagt agctgtttga
    23341 ttttcatttg tttagcatac gcatcattcg tttgtatagc tgcatattgc aatgcgattt
    23401 gtgaacctgc actatctcca ccaaaaaata attgttccat gtctagcaat ttgtcgtctt
    23461 tttcttcagc taataaatct ttgatcagtt cattcacttg aagaacttga ttagggtatt
    23521 ctgaatcagg cgctaattca taattcatcg caaccactgc gatgttggcg tctgaaacta
    23581 attttgtcgc aaattctttg acacccgatt tatctccgcc gacgaatcct ccaccatgta
    23641 cccaaaatag aacaggaaca ggccccttgc tgttttttgg atagtagatg tcataagtat
    23701 tctctttata cttggactca tacgtcaaat ctgttttttt cgccacgttt ttttctgctt
    23761 gatcgaacgt ttttttatca gtgatttgaa cagtatttga aaacatatgg ttgatcaaga
    23821 atgctccagg tctgggggaa agctggaagg caagaaagat tgcaaacgcg atcccaagaa
    23881 tgattccgaa gaaccatccg ataaatttaa gaattttctt tttcataagg ctccctcagt
    23941 agttagtatt aagcattctg attataccat agtgttattt cctatcaagc tctcctagat
    24001 gatttaatga ttttccccaa acttcttctg caatgctttt gacaaaagaa attttttcca
    24061 ttgttcttca aacaaaacat tttcttcttc tgttgaagca aatccacatt gaggacttaa
    24121 gcacaaacga tccaaaggca gatatcgtgt cgcttcgtag atccggtcaa tcacttgctg
    24181 gtgctcttcc atttcgccat ttttcgttac agtcaaaatc taagacaact aatttatctt
    24241 cagagacttt ctctaaaaga acaaaactcc cagcacgttc actatcgtat tctaaataat
    24301 aggcacggac attttcttga gtaaaataat gggtctgcta cgtcgtcatc ccccctgatg
    24361 ccgccaaaat cgaataataa tttccgcgtc aaacatgtat atttagtgtt gaatcatttg
    24421 agaatatgaa aataaaaatc tttttgatca atcagttaat caaattgaaa taacgaaaaa
    24481 tctgagacag aatgacgttc attctgaccc agatttgatt ttgcacaact caacagtcct
    24541 tattggcaaa tcgccaaaat aatgtagact aactaaaaag actaagaaac aataacaaaa
    24601 gttaacactt cttagtcttt ctttagcacg tctatttatt aaagatttta gggccttttc
    24661 tcatgttttt acgatcattc ttaggtgttt ccatttgttt ggatcggcct ccaccttgtt
    24721 tctttttgat caattctttc attttttctt tttggtccat ttttattctc ctttaacgaa
    24781 tgaagcgact aattcgatat gatgggtttg cgggaaaaga tctacaggct gaactttctt
    24841 cacggcataa cctgcttctg caaataactt agcatctctt gcaaaagttg ccggattaca
    24901 tgagatatag acgattcgtt ctggtgacat ttcgattgct gattcaataa aatgatgatc
    24961 taatcctttt cgtggagggt caactacgat cacagatggt ttgatccctt tttcttgcca
    25021 ttttttcata actttttctg cttttccgac ttcataatga acattttcga ttttattatt
    25081 taaagcattg aactgtgcat cttcaattgc ttctggaata acttccatcc catacacctt
    25141 atccactctg tctgctaaag acaagccaat cgtacctatt cctgaataag cgtcgatcac
    25201 aatgtctttt ttatctaact cggcaaactc gatggctgtt tggtacagat actctgcttg
    25261 ttgagtattt acttgataaa atgactttgc tgagattcga taggtttttc ctaatagctg
    25321 atcttcgata tagtctctgc catataaaac tttttctttg tctcctaaaa tcacatttgt
    25381 tttttcttca ttgatatttt ggatgaccga aaccacttct ggcaattctt catgaatagc
    25441 ttcagcaatt tccttggatt taaagaattt ttctttacga gtaactaaaa cgaccatcat
    25501 ttcatgggaa taatgaccac gacggataat gatgtgacga agctgtcctt catttttttc
    25561 ttcattatat cctcgtactt ggaaccgttg caagatatct ctgactactt tgattgcttg
    25621 atcgatgaca ggatcttgga tataaaaatc ttcgataggg actagatcat ggctattctt
    25681 tttgtaaaaa ccggtggcta actgtccatc gatacggcga acagggatct gtgctttatt
    25741 acggtagccg aatggttctg ccattccgac cgttggaagt acaggaactt ctggcatctt
    25801 agcaatcttc gccattacat gctctacttg tttttgtttg aagacaagct gttgttcata
    25861 cttcaaatgg gctaaaggtg cgatacctat gcgaagtaat gcattgtttt ctacaggctg
    25921 acgatcaggt gattgtgtaa taaatgtttc gacttttcca tatccaaact tattgcctgc
    25981 ttttactact agtatttcta cttcttctcc agggagtgca ttttcgataa ataatggata
    26041 accatcgatt tttgctaccc ccattccttc ataactcaag tcgacgattt cgactgtata
    26101 tcgttcgttt ttctttacta tttgctgttc tgtcatattt tgctccttta agcctatttt
    26161 tcaatcctac tgaactctac tataaatcag ctaattacac aactaaaacc ggcactgacc
    26221 ttactacgtt cagcaccggc ttttgattaa ttcttttgat taggtgtttc gtctgctatt
    26281 tttccgtcac cgtcgatatc ttcatccgtt aaacgttcga cttcttttac gaattctttg
    26341 ctgacaacct ctaattcttc ttcatctgta cctgtgattg cttcatcagg aatctgatcg
    26401 gtattggcaa agaactcaat gtgttgatga agattttgga aatgcatcgg tgcttccccg
    26461 ccgtattcac catctaagtt gatcattact ggtcgattga tatcatcttc tgtttcaacg
    26521 aacaaactgc ttgttttggt atagattatt cttggatcat cgacatgtcg accgccattc
    26581 agcatgagtg ctgcaatatg caagatctca aagatatttg ctgtcttcac aatgattaaa
    26641 gaaaacttcc catcatccaa tttggcatct ggcgcgatct gttcgaatcc tccaacagag
    26701 ttcgtcagcc ctaggaaaaa catcgaagca tttccgatat attccccttc atcatacttc
    26761 aagcgcattt tgattggctt tactcgaggg agcatctctg ctcctttcgc aagataagca
    26821 agataaccaa aaatactctt tagttctgat ggaacttcat aagtcaattc tgtcaaatag
    26881 ccacctgcag cgatattgat gaagtaactg ttatcagaaa ggccgatgtc catttttacg
    26941 gtttggtgtt tttttatcac ttctgctgct gctttaatat tatctctagg aatttttaat
    27001 gctcgagcat agtcgttcgt tgtaccagct gggatgatgg ccattttagg acggtgttca
    27061 agcggagcga ttccattaac cacttcgttg attgtgccat cgccaccagc tgccactact
    27121 agatcaaaac ctagttctgc gactctacat gcttcattct tggctgaatc tggttctggt
    27181 gtagttgcat aggcgctggc ttcaaatcct gcttcttcta atgcctgtag gatatccgcc
    27241 aaattttttt tcaataattc ttttcccgag gtggggttat aaattacgcg tgcttttttc
    27301 attttcagcc tctatcgttt cgctaattca tctttaagta gcttattcac gacaccagga
    27361 tttgcttgtc ctttagttgc tttcatgatt tgaccgacta ggaagccaac cgcacgatct
    27421 tttccatttt tgaaatcatc gacagattgt tggttgttat ccaaaatttc attaatgatt
    27481 ggcagtagtt tgttcggatc agataattgc acccaaccat ttttttcaac tacttcttgg
    27541 gcttctccgc catttgtgat caattcacgg aagacttttt tggcgatttt tgagctgatc
    27601 gttccatcgg cgatcaaacg aatcattcct gctaagttct caggtgtcaa ttttgtctca
    27661 gctaattcca gtttctcact gttcaagtaa gcagaaactt cacccatcaa ccagttagat
    27721 gcttgcttgg catctgcacc attttccaaa acagcttcaa aaaaatcaga catttctttt
    27781 gtaagtgtca ataccattgc gtcatactct ggaagtccaa actcacggat atagcgttcg
    27841 cgacgagaag ctggcatctc aggcaaacta gcttttactt tttgaatcca ttcgtcatcg
    27901 atcacgaatt tcgggatatc aggctctggg aagtaacgat aatcactaga cccttctttg
    27961 acacgcatca aaactgtttt attcgttgtt tcgtcaaaac ggcgtgtttc ttgttggatt
    28021 tctcctcctg aaagaagaac ttttgcttga cgtttttctt caaaagctaa accttttttc
    28081 acgaaagata aagagttcaa gtttttcaat tctgctttcg taccgaactc ttcttgtcca
    28141 taagggcgaa gagaaatatt ggcatcacag cgcatagagc cttcttccat tttcacatcg
    28201 ctgacttctg taaattggat aattgatcgc aatgcttcta gataagcgta tgcttcttct
    28261 ggtgaacgca tatcagcttc agaaacgatt tcgatcaaag gcgtgccttg tcggttcaaa
    28321 tcaacgtaag aataaccgcc aataccatgc atgttttttc cagcatcttc ttctaaatgg
    28381 acacgttcga tccggatttt tttcttttta ccttctactt cgatttcgat ccatccgtca
    28441 tgaccgattg gttgatcaaa ttgggaaatt tgatatgctt ttggattatc tgggtaaaag
    28501 taatttttcc ggtcaaaatg agtatcttta gagatttcac agtttaatgc caaagctgct
    28561 ttcatcccga attccagcgc agctttattc attactggca gcacgcctgg ataaccccag
    28621 tcgatcacgt ttgtattgct gtttggttct gcaccaaaat gagctggcgc tggagagaaa
    28681 atttttgagt tggtttttaa ctcgacgtgg acttctagtc caatgactgt ttcaaaattc
    28741 attcgtcttt ccctcctaaa atagctggtt gttgtttatg gaattctgtt gcttgttcaa
    28801 acgcatatgc cgcttgatac atcgtttttt catcaaaata attaccgatg atctgcaatc
    28861 caactggtaa tccttctgat aaacctgccg gtacagacat acctggaagt ccagccaagt
    28921 tgacagggat cgttaagatg tcactcatgt acattgtgat tggatcgtta atgttctcac
    28981 cgattccgaa tgcaactgtt ggtgaagatg gcccgatgat caagtcataa tctgcaaaga
    29041 ctttttcaaa atctcgtttg atcaatgtac ggacttgtcc tgcttttttg aagtaagcgt
    29101 cataataacc cgcgcttaag gagaatgtac ctaacatgat acggcgtttt acttcttcac
    29161 cgaatccttc tgaacgtgag ttgacatata catcatctaa tgatttcaca ttttcagagc
    29221 gataaccgta gcggatacca tcgaatcgtt gcaaattgga acttgcttct gatgaggcaa
    29281 tgatataata aacagccaca ccgtattttg aatgaggcaa gctgacttct tccacaacag
    29341 cacctaatga acggaatgtt tctgctgctt tcaatacagc ttcttttacg ccaggggcca
    29401 caccttcgcc taagtattct ttaggtaaag caatcttcat tcctttgatg tcgcctgtca
    29461 gtccctctgc aaaatcagga actgaaacgc ctgaagaagt accgtctttt tcgtcataac
    29521 cgctgatagc tgtcaatgct aatgcattat ctttcaccgt acgggtcatt ggtccgattt
    29581 ggtcaagaga tgaagcaaag gcaatcaaac caaaacgtga gacacggcca taagtaggtt
    29641 tcatccctac gatgccgtta aaagctgctg gttggcggat acttccccca gtatcactac
    29701 ctaaagaaac tggaatttgt ccagctgcaa ctgctgctgc agaaccacca gaagagccac
    29761 caggtacttt tgttgggtcc caagcatttt ttgttttctt gaaataagaa gtttctgtac
    29821 ttccacccat agcaaattca tccatgttta gcttaccaac agggatcata tctgcttgat
    29881 agattttatc catgaccgtt gcatcataaa ttggttcaaa attgtaaagg atcttagatg
    29941 cagcggttgt caagatatct tttgttacga tattatcctt gataccaatt ggaataccag
    30001 caaggacgtt gctttcatca atcccttttt catctaaagc ttttgcctgt tccaatgctt
    30061 tttcttcgtt caatgtaatg aatgcctcga cctcaggctc tgtttctttg attcgttcaa
    30121 atgttgtttt tgtcaaatct tgtacagtga tttctttcga tacaagcata tgatgcagtt
    30181 cttctagaga acgattgtgt aattctgtca ttatgcgcca gcctccccat tatcgatgat
    30241 tgcgggtact ttgataaagc cgtcttctgt ttccggcaca tttttcaata attcatcgcg
    30301 gttccatcca gcttgcgccc gatcttgtcg catcacgttt actgtttcaa ccacattcga
    30361 cgtaaatgga acaccttccg tatctacttc ttccagcatt tcaaccatat cgatgatttt
    30421 acctaattgg tcggtaaaac ttgcaagttc ttcatctgcg aacgcaagtt ttgctaattt
    30481 ggcaacgtgt ttcacttgtt cttcactaat cgccatacta ccccttcttt ctaaacgagt
    30541 taaaaacttt gctctctatt ttaacacaag agtgccgtgt ctgaaattgt ttattgaaat
    30601 ttgtctgaaa aaactcattt ttcattcttc tactgagcga cttttagcca atgcatctgc
    30661 catttgatct tctgtccaaa cttcgattcc taattcttgt gctttggtca gcttgctgcc
    30721 agcatcttct cctgctacca cgatatccgt ttttttcgat acgctaccag tcactttccc
    30781 gccaagattt tcgatcgtct cttttgcttc ttcacgagta aatcgtgtta gtttaccagt
    30841 aaggactact gttttttctt taaaaggaga ctcgacttct tgcaattggg catttcgaag
    30901 ccctttgtat tcgaaattga cgcctgcttg ttttaattca ttcatcaatt catggacttc
    30961 ttcgttactg aaataagtga ctacactgtc ggcaatgata tcaccgatcg tatcaagcgc
    31021 aataatcgac tcatagtcac ttttactcaa tgtttctaga tcaccgaaat gttctgctaa
    31081 aatttttgct gctttcgctc cgacatgtcg gattcctaac ccgaaaatca accgctcgac
    31141 cgaattatct ttgctgttgt cgattgctgt caaaatatta tttgctgatt tttcttttat
    31201 tttgtccaac gtcaataact cttcttctgt caatttgtaa agatcagcaa catctgctac
    31261 tagtttttta tcatacatct gttctagtac gcgcggcccc aacccatcga tattcatcgc
    31321 atttcttgaa acaaagtggt tcaaaccttc tttcatttga gcagggcatt taggattgat
    31381 gcaccgtaag gctacttctt catccaaatg gacaagttca cttccacaaa ctggacaatg
    31441 tgtaggcaat tggtattctt cgctgtcttt aggtcgttta tctaacacga cttgcgaaac
    31501 ttctgggatg atgtctcccg ctttataaat cagaactgta tctttcaagc ggatatcctt
    31561 caacttaata taatcaccgt tatgcaggct ggcacggctt actgtcgttc ctgctactcg
    31621 gactggtgtc atgatagctg ttggcgttac tacccctgtt cgaccgactg tccattcgat
    31681 gttttcgatt agcgtttcaa cttcttctgg cggaaacttg taagctgctg cccatctcgg
    31741 tgctttgata gtaaaaccaa gttgatcttg tagcgagaat tcattcactt tgatgacgat
    31801 tccatcgatt tcatagggca aatccacacg tttgtcatga tattcttcaa tgtatgacca
    31861 cacttcatca atcgaatgac atagacgttt ttcccgattc gtgtggaatc caattttttc
    31921 tagctcttct agcgcatcat actgggtttt tgcttgcata ggaccgaaat cagctactgt
    31981 gtataaaaaa gtgtccagat ttcttttcgc tgtgatttta gaatctaact gacgcaaaga
    32041 accagctgcg gcgttccgag gattcgcaaa gatatctttt ccctctgctt ctctgtcttg
    32101 gttcaattgg acaaatgacc gttttggcat gaaacattcg cctcttactt cgatgttcat
    32161 tggttctttt aatttgattg ggaccgaccg gacagttttt aggttttcag tgatgttttc
    32221 tcctacagtt ccatccccgc gagtcgcacc tctcacgaaa tttccatctt cataacgcaa
    32281 agaaacggaa agtccatcta tttttagttc acagcaatag tcaactacac gtcccactgc
    32341 tttttgtaca cgttgatcaa aagctatcag ctcttctttg ctgaaaacgt cgttcaaact
    32401 gtaaagcgga atatcatgag tgactttttc aaaaccttct aatacttttc cgcctactcg
    32461 ttgagtggga gaatcagacg tgatcaaatc aggatattgt tcctcgattg ctactaattc
    32521 tgcgtattct ttatcataaa catagtcttc cactgttggt ttatcttcta cgtagtattc
    32581 gcgagaccat tggttcagtc ttgtacgaag ttcttctgct cgtttagcag cttcgtcaat
    32641 cgtcatttca tgttccatca tttaccctcc actactaagc tttttcaatt ggcgcaaagg
    32701 cagccaaaag acgttttact ccttgacttg ggaaagccac atctagttct agatccttcg
    32761 acgtcccgct tactcggacg actgttccta gaccccattt tttgtgtttg actttatctc
    32821 caggtttcca gctgttattt tcccctccag tagcactttt agagggaaca accgctgtct
    32881 ctggctgttt atagctatat gctgtttttt tagcgaatac aggcgttttt ttaggcgtag
    32941 gacgcatgcc ttcgatttcc agtaactcct gatcgatttc ttctacaaaa cgagaaggtc
    33001 ggttatattg cgtccgtcca tataatgttc tggagaaagc atttgttaaa tacaacgctt
    33061 cttctgctcg cgtgatcccc acatatgcga gacggcgttc ttcctccagt tcgctttctt
    33121 ccatcaatgc tcgagataaa ggaaaaaccc cctcttctag accaatcagg aatacaactg
    33181 gaaattctaa tcctttggct gcatgcaaag tcatcaatgt cacttgggaa gcatcttctt
    33241 ctaaattgtc aatgtcggaa acaagtgcca gatcatttaa gaagaccgtt agtttttctt
    33301 ctggtgcatc agcgtcttct tcattttgct gttcgaattg tttgtcgaat tcttgtgtga
    33361 ccgttaaaaa ttcctccaag ttttcgatgc gtgcttgtgc ttctaatgta ttttggattt
    33421 ttaagtcttc tagatatccg ctacgatcca aaacctcttt cgtcaattct gtcactgtca
    33481 agtatgggat catctgagtg acttcttgga tcatctctcc aaatgcacct aattgctgtc
    33541 ctgcttttcc accgatattt gccagatcca cattttgcgc tgcttcaagc agtggccatt
    33601 catgaagaga agcaaaactg cgtagttttt cgatagatcc tggtccgatg cctcgtttgg
    33661 gcgaattaac gatacgctca aaactgattg aatcttgagg atttaccagc acattcaaat
    33721 acgctaaaat atctttgatt tctttacgat cgtagaactt gtgccctcca accattttat
    33781 aaggaatatt tgctttcaac agagtttctt ccattacacg agattgtgcg ttcgtccggt
    33841 acaagatggc aaagtcgccg taattccgat gattgcttcg aatctcttct tgcatccggt
    33901 ctacgataaa acgggtttcg tctcgttcat tatctgcacg gtagtaagtg atcttgtttc
    33961 cttctttgtt ttctgtccac aagttttttg gttttcgatt gctattgttt tcaatgactt
    34021 ggtttgctgc gctaaggatg tttttcgtgg agcgatagtt ttgttctagc aaaattactg
    34081 cagcatctgg ataatctttt tcaaaatcca agatgttctg catatctgca ccgcgccagc
    34141 cgtaaatact ttgatctgca tctcccacga cgcacaggtt cctaaatcta cctgccaata
    34201 aattgactag cgtatactgt gcgtgatttg tatcttggta ctcatctaca tgaatataat
    34261 ggaatttgtt ttgataatac gtgagtgaat caggatgttc ttcaaataat cgaatcgtat
    34321 tcatgatcag gtcgtcaaag tccatacatt gattatattg aagctctttt tggtaagcag
    34381 cataacattt tgccgcaatc tcttcgtaat aactgccttg catttttgca aaatcctgag
    34441 gggtctgcag gctgtttttt gcttgagaga ttgttcctaa tatacttcga ggatcatatt
    34501 ttttcggatc aatgttcagt tcttttagta tccgtttcat caaggtcagc tgttcagaag
    34561 aatcgatgat tgtaaagttc cggttatacc cgataaaatc aacatctctt cttaaaatac
    34621 gcacacacat cgagtgaaaa gtcgataccc agacatcttc gcctccagat gcgagaatcg
    34681 cattgacacg ttctttcatt tcttttgcag ccttattggt aaaagtaatc gctaggatgt
    34741 tccaaggatt gacttctttt tcctcaatca aataggcaat gcggtgtgtc agcactctcg
    34801 tctttccact tcctgcacct gccattagca gtaatggtcc gtcagtgtga agaacagctt
    34861 ctttctgacg ggggttcatc ccgtttaata attcagcttt ggatgacatt aacaacatcc
    34921 tttcttttat caatcttgtt aattatagca ttttcttggc tttcattaaa ctagctagtt
    34981 caaaatcatt tgtttctttt tatggatttt tccttgtcaa ttcgttgatt tcgcgctaaa
    35041 ttaaaagaaa gcgaggcgtt tgaatggaaa aaaaaggaaa aatcaagaaa ttagataaga
    35101 ctcagatata ttttattcta aaaggaatat ttgttggact gctgacagga atcgtcgtta
    35161 gcctgtttcg attatctatc gaaaagctaa gcgatttagt tcgaagtatc tatgaaatga
    35221 gtcgggaaca gccgatttac ttaattggcc tagctatttg ctgcatcctt gcagcatttt
    35281 tcgtgggtta tctggtcaaa aatgaaccgg atatcaaggg aagcgggatt ccacaagtag
    35341 agggacagct tcgcggggaa ttatcaatga actggttctc tgttttatgg aaaaagttca
    35401 ttggcggtgt actttcagtt ggtgcgggcc tttttctagg tagagaagga ccttctatac
    35461 agcttggcgc atccattgga caaggagctg gacaattgtt tcggagtcca tcttcagaag
    35521 aaaagatttt aatttctagc ggtgcaagta gcggacttgc agcagcattc aatgctccta
    35581 tcgcaggatt actctttgtt ttagaagaaa tccatcactc cttttctcca cttgtttggc
    35641 tgacatcatt tgcttcagca atcactgcta acttcgtctc tctttacttt ttcggcttga
    35701 gacctgtttt atatctaggt gaccttcctt ctttgccgtt gaaatattat ggttccttag
    35761 tgatcttagg agtacttcta ggcatcttag gttttattta tcagaatgtc ttgctggctt
    35821 tgccaaaatt ttacaaccga ttgcctttac cagcttattt tcacgggttc gttcctttct
    35881 tgctgatttt gccaattggt tatttatggc ctagtactct aggcggaggc aatcaaatcg
    35941 ttttagcttt tggacatgct tccttgccaa tcattatgat tatcggtttg tttgttcttc
    36001 gattcgtttt ttccatgatt tcttacggtg ggggtcttcc aggggggatt ttccttccga
    36061 tattgacatt gggggcttta atcggtagct tttatggaaa cattatggtt gatttcggga
    36121 tggatccaat ctacatcaag gattttgtcg ttattgccat ggctggttat ttcacagcga
    36181 ttggaaaagc accgctgaca gcaattattt tagtgacaga aatggttggc tctcttaatc
    36241 atctgatgcc tttaggattg gctgctcttg ttgcttatat cgtcaatgac cttctcggcg
    36301 gtaatccaat ttacgaatca ttattggaac gtctacttgg aaatactcag accgcctcac
    36361 taaaaggtgc aaggacaact tttgattttc cagtaacggc agagagttct ttagacggat
    36421 taatggtacg agacttcaat tggcctaaaa atatgctgct aatctctata cgcagaggaa
    36481 atcaggaaat cttgactcac ggtgatacga tcatgaatgt aggagatatt ttggttatct
    36541 tgactgatga agcaaacttg ccaagaatca agcaggaaat cgaggctaaa tcttctatca
    36601 caaatttgaa gcaatcattt taacgaacat ggaaaaacta ttttacaaca actattcatc
    36661 tacactactt gattctatat gattctataa aaaaagaaga ggctgggaaa acccagcctc
    36721 ttctttttta tttctatgct tctgctactg caggagcttt tctcaataaa ccataaatca
    36781 caccagatac gactgctccg atagcaataa acaccaaata catgaccggg tgactcagta
    36841 agaatacaac gaaaatcccg ccgtgaggag ccagtaattt gattccgaaa gctccgacta
    36901 atccgccagt taaagcagaa ccaataataa aacttggaat ggcacgaatc gggtcagctg
    36961 ctgcaaatgg aatcgatcct tccgtgacaa acgatagtcc catcacgatg ttcgtcaaac
    37021 cagcatcttg atctgttttg ctgaatttat tcttaaacaa acgtgtagca acgaaaattg
    37081 ccaatggagg taccattcct ccagccataa cagaagccat cacgacactt ccgccagttg
    37141 caacagtcgt agctaaagtt gctgtaccaa acacataagc ggctttgttg attggaccgc
    37201 ctaaatctgc cgccatcatt ccagctaata atgcaccaag taaagcagca ttcgaaccac
    37261 tcatacttgt caagaaaccg ttcaatgcat cattgattgc tttcataggg acattgacca
    37321 gcaacatcaa gaaaccagta atcagtaagc caaacactgg ataaaagagg atcgttttaa
    37381 ttccatccaa tgattttggt aagttcttga ataatttacg taaaaatacg atgacataac
    37441 cagctaagaa tccaccaatc agtgcaccta agaatcctgc accgcctgta ttagctaatg
    37501 caccagcagc aaatccaacg atcaaaccag ggcggtcacc gatacttgaa gcaataaatc
    37561 ctgctaatac cggcagcatg aaaccaaatg cagcttgtcc gatttggttg aaccagctag
    37621 cagcttgatt gtaattccct agatttgata attgatcatg tggcacgcca ataaattggt
    37681 cgatcatgaa agaaagcgca atagcgattc ctccaccaat aacgaaaggt aacatgtgtg
    37741 atacgccgtt catcaaatct ttgtaaattc tttgtccgat agtaccatcc gcatttcctt
    37801 cttctgaact gctttctttt ccatcaccat ggaatactgg tgcagtacca ctaagggcta
    37861 gattgatcaa ctcttctggt ttacggatac catcactgac aggacgattg atcagttctt
    37921 ttccgtcaaa gcgattcatc tctacttttt tgtctgctgc aacgattaca ccttctgcac
    37981 gggcgatgtc ttcagcagtc aatcggtttt tgattccttc tgaaccgttc gtttcgactt
    38041 tgatctccac acccatttct ttcgcttttt tcttcaaagc atcttctgcc atgtaagtgt
    38101 gagcgatccc tgttggacaa gctgtaacgg caacaacgaa ttttctttct ggatgagcag
    38161 aagcttctgc agcttgtgtt tcttgttctg ctttttcttc tgcttctttt tgctcttcag
    38221 ctgtttggaa caagtcttgg acttcttctg gtgtatttgc ttcttttaat tttcctacga
    38281 aatctgcatc aatcagcaat cgagatagtg ctgctaatgc ttgcaaatgt gtgtcgtttg
    38341 cgccttctgg agctgcgatc atgaaaaaca gataagttgg ctgtccgtct aaggcttcat
    38401 aatctactcc tttttggctt tttgcgaata aaactgttgc ttctttaact gcactgtttt
    38461 tcgcgtgagg catggcgatt ccatcaccaa gacctgtaga agtttgtgct tcgcgggcta
    38521 gaattccttc tttatatgtt tcgatatcgg aaatccggcc ggcatcaaac attttttgga
    38581 ccatttcatc gattgcgcct tttttatcag tcgcttgcaa atccatgatc atcgcttctt
    38641 tgaccattaa atccttgata ttcatgatta tcctccttga aactaatcaa tttttgtgat
    38701 ttcaacttct ggcagcaact catcaatcaa ttcgcgagct gccaagtcat cagaaaaagc
    38761 tgttgcgctt ccgcaagcaa ctccccattt aaaggcttcg attggatctt tcgtacggct
    38821 gtaagcccca ataaagccag caatcattga atcccctgca cctactgaat ttttcaatgg
    38881 gcgaactaat acattcgttt tataagttcc ttgttccgtg aagagcaaag caccgtctcc
    38941 agccattgag atcagcacat tctgtgcccc ttcagctagt aattttttcc catacggaat
    39001 gatttcttct acagattgga aggttgtgtt gtaaagctca gctaattcgt gattgtttgg
    39061 tttgatcaac aacggtttct taacaagtgc attttttagg tcatcacctg ttgtatcaat
    39121 cacgaaatct gcaccttttt ctcgaatgat atcaatcagt tcttgataaa atccttttcg
    39181 caggctagct ggcgcgcttc ctgacaatac gacgatatct ccttgtccta ctgttcctaa
    39241 tgcttgcttc aactcatcga tttcttcatc tgaaatagat gggcctaagc cattgatctc
    39301 agtttcagtg tctgatttta gtttgatatt gattcttgta tcttctttta cggttgtgaa
    39361 caaagactgg atttgttctt tttcaagcca cttcgaaata aattcgccag tgaaccctcc
    39421 taaaaaccct agtgctgtag attgagaatc gatccgtttc aaaatacggg aaacattgat
    39481 cccttttcct ccaggtaact tgaagtcttc cttcattcgg ttcaaatcgc ctagttttaa
    39541 cccatctacg cgtacgatat aatcaattga tggattaagt gttactgtgt aaatcattgt
    39601 attgcctcct taattgttgt cttttcacgg aattcattaa cgaattctaa tggacaacgc
    39661 tcggttatga tcgtggctgc ttccaaaggt gcaacagagg tgaaggacaa ctggttgaat
    39721 ttggtatgat caatcaatac aaatgtctct tcggaatttt tcatggctaa acgtttaaca
    39781 gctgcctctt ctggatccgg agtcgtgaat ccttgttcaa gatgggcacc gttcattccc
    39841 ataaatgctt tattaaaatg aaaatttttt agttgttcta tcgcattaga tcctaaaaca
    39901 gcattagttg ccagctttat cgttccacct aaaataatgg tagcaagctc caaatcaact
    39961 aaatttgccg catgttgaac ggaatttgtc actactgtga tctgttttcc ttgcagaaaa
    40021 ggaatcattt ctaacgtagt agagccagcg tctaaataaa tgacatcttc gtcattaatg
    40081 gtttcagctg ccgatttggc gatttgctgt ttttcgtgaa cgtttttgag tgatttttca
    40141 ctcatattct gctcaaaatt caataattgc tctttcttag ctccgccatg aactctttct
    40201 agaaacccac tattctccag ctcttgtaaa tcacgtctta tcgttgattc agaagcattc
    40261 aaaaggttta caagatcttg tgatttcacg attttctgct gttcaagcaa gtctaggatt
    40321 ttttgtctgc gttcctctgt aagcattttc acgatcacct caatacaaat aatagcatat
    40381 tttttttcta ctagcaatat aaatcagtca aaaacgttca taaaaattat aaatcagtca
    40441 aaaaaaaata aaaaaccagt cagaaagact ggttttttat ttttttgtct atcatcaagc
    40501 cgttttatcg ttttttattt tcgcttcttg tttttttcat ataaacagaa gcaggttttc
    40561 tttccttatg aagaagataa taataaagtc ccatcccgcc aaacatgacc aaaaaaccga
    40621 tgactgatcg aataggaaaa ttaggaacaa aaaagagaca gacggataat aatgatgccg
    40681 gaaccattat tcgataatag agttgtttat ccattttctt cacctcatat tttttgtaaa
    40741 gtaatcaatt gttctaattc tcggtaatct aaaaattgca cacgcagatg ctgctcacct
    40801 ttcttgacat cgaattggat acttcgccgc tttttcttta ttcgtttgaa ggtaacatca
    40861 gagagtctat gtttgatttc ctttaacggt aattctgaca gggattctga aggaacatag
    40921 attcttaagg ataaaagata ctcttgatat ttttcatctg ttacctctac ttctagatat
    40981 tccctaatat aataacaata agcttcgatt ccattatcca aagcaacagt cggatcgata
    41041 actttatagc acatcatgcg tgatcgattc tcgtcaaccc aaggaaaata cgcctgagca
    41101 tttagtgttc cgcgaacacg cctttcttta taatctcctt gttggatatc ataacttttt
    41161 gttacaaaaa atgaagcatt ctttttagga acaggttgca tttgtgcatt ctgatactgg
    41221 acagattgga tcgtatggtc agtgtaaatg tattcaacga atggcggttt caataaaccg
    41281 acaccattgt cccaagcaag atgatcaaac ccaagctggt ccctaaaata ggattgaaac
    41341 atttgattgt ttggcttttg ccagttaaag gggtctcgat aaagctggat acatatgact
    41401 tcgttttcta attccttcac tttttctact tgttggatgt cgagaaaaat cggtttaggg
    41461 atgatttgat gataatagcg actataatac atttcccaac acgaagtgaa acataaacct
    41521 tggtaaaaaa gatcgtatcc tggaaattgg ttgcaatcga ctactacttc cccgttttgt
    41581 tgtttcattt taggtaaatg ttcgatttca tcctttttct caaacaatag ccgttgagag
    41641 atctgttttg tattgtgata gataaactcg ctataagctc gcatataggc gaacacccca
    41701 tgagcaatca tttttttctg aatgaagtct cgaatcaaaa attgcttttc tttgaaaaca
    41761 gagatagaca atacattttt tatcaataat tgattatctt ccaagcgaaa actacattcg
    41821 ttctcgtcat ttcctacaac taaccaactt tcgtctctta ccgatgctag cattattttt
    41881 tcttgaagct ttcgaagatc atacggatcc gcttgttgtg tgaaagagcg gataaatgtt
    41941 ggctggatca acagacgtgt caaaatagcc caatcgctta tttcctgttt tttttcttct
    42001 tcatttaagt ctgtgagctg gatagtcatc aataatttat cttcgccaat cattttctca
    42061 cctctttcaa atctttttat aatttaatta taaccaatga taaagaaaaa ctaaaacttt
    42121 atgtcttaga aaccaatttg gacgattaca tgatagacta agtaaaaaga aacaaagagg
    42181 tgtttacaat ggggttgaaa tttgaacgta cagataattt agataaactt ttcgaatcat
    42241 ttgctgtcga tccggaaaaa acagttgcac caaaagaaga gatcgatcga tttctaaaac
    42301 cacagccaca aaatgaaaag aaaaaagaca agaaacaaaa gtaagttaag ctcaaaaaaa
    42361 gacttaccag ccaaactttt atgaaagagt ttgactggta agtctttttt attcttttta
    42421 tgattcaatt gcttgttcga ttgctgccca cgcttcttct tttccctgtt ttgtaacgga
    42481 agaaaagaga ataaacgtgt cattgcgatc aaaatccaac gcttttttga tttcgctctc
    42541 atgcttgttc catttaccac gagggatctt atctgcctta gtggcaacaa caattacagg
    42601 gatatcataa tacttcaaaa attgatacat ctggatatct tcttgagaag gcttatgacg
    42661 caaatctact aaagaaacga ccgcacgtag ctggtctcta gtcgttaaat atgtctcgat
    42721 catctttccc cattttgctc gctcagtttt ggatactttt gcatacccat atcccggaac
    42781 atcgacaaaa tgtaatgcgt tctcgatcaa ataaaaattc aatgtctggg tctttcctgg
    42841 cttaccggaa gtacgggcta aattctttcg atcaatcaat gtgttgataa aagaagattt
    42901 ccctacattt gatcgtcctg ccagagctat ttctggcaat tgtgtatctg gatattgttt
    42961 tggtgccact gcacttatga taatttcagc ttgatggact ttcatggtct ctctccttaa
    43021 caaggtgatg aataaagcaa gcggctgtcg caaaagccaa aatgactttt acggcagcct
    43081 cttttcatca acccgctttt ttctctgaat ctttatagat tactgttggc tcaccagaca
    43141 attcagctgc ttcttttgtg ataatcactt tttcgatgtt ttcatttgaa gggatatcaa
    43201 acatcacatc catcatgatt tcttcaatga tggaacgaag tcctcgagca ccggtattgc
    43261 gttcgattgc ttttttcgca atggctctca acgcttcagg ttcgaattca agttcggtat
    43321 tatctagaga aagaagtttt tgatattgtt tgactagcgc atttttcggc tcagtcaaga
    43381 tccgtaccag atcatctgtt gtcaatttct ctaaagctgc cataacaggt aatcgtccga
    43441 taaactcagg gatcaatccg aattttagta aatcttcagg aatgatatgc tgcatgacac
    43501 tttcgtcttc agaaagtttt tggttttgtg taccaaatcc aatcgtcttt tcgcccatac
    43561 gattcttcac aatcgtttcg atgccatcaa atgcaccacc tacgatgaac aatatgttcg
    43621 tcgtatcaat ctgaataaat tcttgatgtg gatgtttacg tccgccctga ggtggcacac
    43681 ttgcaacagt tccttctaga attttcagca aggcttgttg gacaccttcg ccggaaacgt
    43741 cacgagtaat agatacattt tcgcttttac gcgcaatttt atcgatctca tcaatataga
    43801 tgatcccttt ttctgcaagt tccacattgt aatcagcaga ttgaagcaat ttaagcaaaa
    43861 tgttttctac atcttccccc acataaccag cttctgtcaa acttgtcgca tctgcgattg
    43921 caaaaggtac attcaatgtt tttgctaatg tttgtgctaa aaatgtttta ccagaccctg
    43981 taggtccgat caagcagata ttgctttttt gcagttcaac atcatctgtg ttttcttgtg
    44041 cttgctgatt gacacgttta tagtgattgt atacggcaac agctaacgag cgttttgcgc
    44101 gatcttgtcc aatcacgtag ttattaaata cttcaagaat ttctagtggt ttagggactt
    44161 cagtgaattc gcgtacagct tcttcataaa attcttcatc aatgatttct ttacataggt
    44221 cgatacattc attacagatg tagacccctg gaccagctac gatctttttc acttcttctt
    44281 gggttttccc gcaaaaagaa cagcgtacgg tctcattgct acttggattg tcatacatgg
    44341 tcttcacccc ttaacttgca aaaagtatgc aagaaactat caaagacttt acagtctttt
    44401 actatcataa catagtttag taaaaagaaa aagtgtttaa caaaggatca actgacctat
    44461 acctttgcat tttttactct taacaaaaaa taaggaactg tgacaaaatc gtgtcacagt
    44521 cccttatttc gaataaacaa ttttctagaa gtagcttctt cagaaatgat ttattacttg
    44581 atttctattc tttcgcttct gtcgctgtac ctgtgatcaa ttcgattgct tttttcattg
    44641 tcacatcatg ttccagcata tcttctgtca acacgcgttt gatttgttcg attggcatgt
    44701 tgtatgtttc tgataattct tggatttctg cattgatttc ttcttctgtt gcttgaagat
    44761 tttcagcagc tgcaattgct tcgatcacaa gatttgttct tgtgcgcatt tctgcttcac
    44821 cttcgaattg tttatgcaag tcttcttctg tagaaccagt taattgatag tacatttcag
    44881 gagagatacc ttgacgttgc atgttgttca agaattcgtc cattgaacga tgtacttcat
    44941 catgaaccat cacgtgtggc aattcaacga tttctgcgtt ttcaacagcc atacggatcg
    45001 ctgcttcatc ttttgcatcg tccgctgctt tttcttttgt ttctactaat tctttgcgat
    45061 atttttcttt caattcgtct aatgttgata cttcgtcatc aacatctttt gcgaattcgt
    45121 catctaattc aggcacttct tttgctttta cttcatggat tgtcactttg aagactgctt
    45181 cttttccagc aagatcttct gcttgataat cttctgggaa agttacattt acatctagac
    45241 tttcacctga tttatgacca actaattgat cttcaaaacc tggaatgaat gagcctgaac
    45301 ctaattcaag tgagtaattt tcgccttttc cgccatcaaa cgcttcgcca tctacaaagc
    45361 cttcgaagtc gattactact gtatcgccat tttcagctgc agcatcttct ttgataacta
    45421 attcagcttg tgcttcttgt tcacgtttga tacgtgcatc cacgtcagca tctgttactt
    45481 cacggtcttg tttttcaaca actagatctt tatattgtcc taattttact tctggtttca
    45541 ctgttacttc agcagtgatt acccagtctt gacctttttc catgctttca acatcgattt
    45601 ttggttgaga aacaggatcg atgccagctt ctttgacagc agcttcataa gcttctggta
    45661 atacagcatt caatgcatct tcatataaag cttcttcacc gtacatacgg ttgaatactt
    45721 gacgagaaac tttcccttta cggaatccag gtacgttcaa gctttttttc actttattaa
    45781 acgcagttgt taatcctttt tggatttcgt cttgcgcaat cgtaaatttt aaaacaccat
    45841 cattcgtgcc ttttttttca aatgttgcag tcatttaatt ccctccgagt ttagaaattc
    45901 attttataca tcaaaaacgt ttcatgcata cctttaaata gtacactaac cctttcaaat
    45961 tgtaaacata ttgaatggat agaatccttg tttttcataa catttttatc tttttcactg
    46021 ataaaatcta atggaagaag atcatttgct gcaaaatttt ttctcttttt tttaaaaaat
    46081 cttgtttttc atcttctgta ccttcataca tcgtttttcc tgtacacgcc tctacagtca
    46141 gttgaaacca gcgcttcggg tcttcgatgt atgtatcata gacaggataa acaattgcag
    46201 attgcaacgt gaattcttct tttaacacgg aactaagcag cgcatcttca ttcccgtaac
    46261 gatctgaaaa tacacataat tgctggtaac ttgcctgctg ctgcggcttt ggcagatctt
    46321 ttggtatgac tttgactaac ttttcttcaa tcgtcaaata gaaaacagga tggcattcgt
    46381 tcaattgcct aagtgtctcc agtaattttg cccttgctaa aatatggacc tctggcgata
    46441 caataagttt actcgctgtt tggataaact catgataagg aagctgttcg atctcatcaa
    46501 tagacgcaag ctgacgtgca ggttccaacg aaggaagttc ctccgattta ttgtggagtg
    46561 ccttgatctt ctgcctctcc atttgtccgg ataattcttc tgcaaattgg attttttcaa
    46621 tgacccgctg ctcgaaagct tcataaaaat ctgtacttgc tatcaatttt ctagcatata
    46681 aaaaatcttg tgtactgatt aacaagtcaa aataaaattc agcaaactcc tcattttgaa
    46741 gatactgttt ttcatgcaag agtgcttgct tcaatgcttc ttttttctcg tccaactcgt
    46801 aaagacaaaa aacaagcagg ctattagcct caaagctttc tttcaactcg taagctcgtt
    46861 gaaataattc ttttgcctgc tgatattgct ggtttgctag tgcttgttgt gccactcgca
    46921 ttaaattctc atatttttct ggaaaatcaa tcggcttcac gcagtgtcct cctcttgctc
    46981 atattcagct catgttcaat agtagcaaaa caaaaaaagc atgacaatta aaaataactg
    47041 aagcaccctc taaaagctag attttagatc tgacttttag aaggtgcttt caactgttac
    47101 tgctttttat tgctcatcag ctgttgactt cctggttgaa cgctttgatc agattgatcg
    47161 taacaacact tgtattagtg aaaaaatcgc ccagatgttg ctttttctga ttaaagactg
    47221 ctggaagata ccctaacatc agcaaaggaa attttagaat gaaccggcaa gcaccttctc
    47281 taactaaaac ggttgaccag ctaagttctt cttcatcaaa gctgatgact cgaataccaa
    47341 agatcatttt cccaatcgtt tggccatgat tgaatttcgt caataaaata aaatacgcta
    47401 gataaataag taaggctgtg aatccgtaga gactcagata agaatctgaa aaattaagcc
    47461 aacctagatt tttggcaata ccaagtgtta aacgcgtaat agctccgata cacaaaagat
    47521 cgaccaagaa ggcaaacatt cggataaaaa aacctgcata gaaatactta ggaaaatcat
    47581 ttgcttttgg cggttcattt cttgtgtccg tatacttttt ccatttacgt tgtttctttt
    47641 tgatttcttc gggcgtcaaa ggctctcttt caaaagagct cagtaccttt tgggaaaagt
    47701 tggagtcagg tcctacagca ggctgctgtt gcttatcttt ttcttgttcc ttattttcat
    47761 ttgaaagctc tgacatgctt actcacctcc gtaataatac attgcttttg gtgattccgc
    47821 tgtccctaga ttctcaagaa cggataagat ctgactagtc tcagaaggct tcaatccctg
    47881 gaattctgct agtttacttc ctaaccatgt actagcaaac ccagatggcg tggaagaata
    47941 ttctactagt tccgcatctt caagctgttg ttcctttcgt atagctttta aagcatcttc
    48001 aggaaatccc aattcatcga ctaaacctac ttcttttgct tggacaccat cataaatcct
    48061 tccatcagca atttttttga cttcttcttc agatttattc cgtccctcgc tgacgattgc
    48121 tacaaaacga ctatatgcgc tgtcgatata tgcttgaagc accgcttctt cttcttttgt
    48181 ctctggtcgc gttcctgaac ccatatcttt caacgctccg cttttaactg ttgtatcttc
    48241 cacacccagt ttttctagaa gtcctgaata attcaatcct gacatgatca caccaattga
    48301 gccggtcact gtttcttctg ttgcaaagat tttatctgct tgagcagaaa tatagtagcc
    48361 accgctagct gccatgtttt tcatgctgac atacattggg atatcatgtt ctttacgaat
    48421 cgtatccaat ttcttagcga tttccgcact ttcgtagaca ccgccaccag gagaatttac
    48481 ttctaaaaag atacctttga ctgttggatc ttcttcaatc gccttcagtt gttccataaa
    48541 atttttatga ttgtaacctt cagaagcaaa taaacctgaa gaccctcccg cagcaatcgt
    48601 tccatctact gataatttta cgattttttg cgtactgtct ccctcttcca acacatttgg
    48661 agctagttct tcatccccat ataaccaact gttcaatccc tcaatttgat cgttttcctg
    48721 aggcttagtc aaactagctg aaactaacga aaccacaaat aatcctgcag caatcagaac
    48781 tgctgcccaa cgtcttttat tcattctttc tcctttctaa tacaagtttt ttatgacgat
    48841 cacagtttct gactgctcag aagtgtcgaa ccgctctgct ttttcttccc gcgcataccc
    48901 ggaaaacgac tcttttgtta attcatcttc taccgtaaac tcacgagctt gagcaacttc
    48961 caaggcatca ggtgcgattt cttggatgct atcccagcca gtatctaaaa atattttcgt
    49021 atcatctgat acaccagctg atgcttcgaa tttggacaat tgttttttta acattcccag
    49081 tgtcatgatc tcaatttttg aatccatttt tactcctcct ctatccgatt actttctatt
    49141 gtaccgaact cctgaaaaag atgatagata aaaacccatt attttttgac ataaaaagga
    49201 catttgacaa agaatttgat atataccaat ttatttttta ttcttttttt gttgacaaga
    49261 acattcgcaa aaggttatga ttttaaatgt ttccagataa aaaacaaaaa ggaggatagt
    49321 tatacctttt aggatagcgc caggtctgtg taaacagatg acgaggagag ggtttatcga
    49381 aagattcggc ggatggccct agggactgca ctctacagaa aatccacaaa aaatgtctgc
    49441 aaagataaaa caaaaggatt ttcctgcagc tggaagcatt catacgaatt cattatagaa
    49501 agtaggatgt tttttatgtg cggaattgtt ggaatgattg gattaaaaaa tgtaacacca
    49561 ggattgattg acggtctaga aaaattagaa taccgtggtt atgattcagc tggtatcttt
    49621 gtctcagatg gcacaactga ttatctggtc aaagcacaag gcagaattca aaatttgaaa
    49681 aacaaaataa atattgatac tacaggaaat ataggtattg gtcatacacg atgggcaact
    49741 cacgggcaac cttctgaaga aaacgcccat ccacatactt cccaaagtgg acgcttcgtg
    49801 ctcgttcaca acggcgtaat cgaaaacttt gaagaactta aaattgctta tttagcaaat
    49861 gaccacttta ttggtgagac cgatactgag attatcgctc atctaattga aactttcgcg
    49921 aaggatacta caacgcaaga ggcgttttta aaagctttgc gggtcatcaa aggttcttac
    49981 gctttcgctt tgattgatcg gacagcacct gatgttattt acgtcgctaa aaataaaagt
    50041 ccactattaa tcggactcgg cgatggattc aatgtgattg ccagcgatgc aatggctatg
    50101 cttgctcata cgaaggaatt cgttgagatt gaagatgaag agatggttac cgtcacatca
    50161 gaaaaagtta ttattcaaaa ttttgcaggt gacatcgtag aacgttcttc attcgaagca
    50221 caagtagatg cttcagatat cgagaaaggt acctatcctt tttatatgct gaaagaaatc
    50281 gacgaacagc ctattatcat gcgtcgtatc gctcaaaaat acgtaacaga agacaatcgt
    50341 gtctctcttg atcaaaagct ggtcgataca ttgtctgata gcgatcgaat ctatatcgtt
    50401 gcttgcggaa ctagttacca tgcggggtta gcaggtaaac agactttgga aaaattgaca
    50461 cagattcctg ttgaggttca tttagccagt gaatttggct ataacacacc tcttctttca
    50521 aaaaaaccat tctttatttt cttgagtcaa agtggcgaaa cagcagacag ccgccaagtg
    50581 ctggtaaaaa tcaaccgtct cggctaccct tcacttacga ttacaaatgt tgctggttct
    50641 actctttcgc gggaagcatc ctttacactg ctattgcatg ctggacccga aatcgcagta
    50701 gcatcaacga aagcttatac tgcacaaata gctgtactaa ctttattagc caaagctatc
    50761 ggtgataaaa aagaatttga aacttctctt gtattcgata tcactcatga attgagttta
    50821 gttgctaatg cgatggaatc ggtcatcgcc caaaaagatt atttagaaga gttggcagaa
    50881 gtatatctca gcgatacaag aaatgccttt tacattggac gtggtgctga ctacaacgtt
    50941 tctcttgaag ctgctttgaa attgaaagaa atcagttata tccaggcaga aggttttgcc
    51001 gctggcgaat tgaaacatgg aacgattgct ttgatcgaag aaggaacacc agtgatcgga
    51061 atcatcagtg aagaagtgac tggtgcgcat actcgaggaa acctgaaaga agtcgaaagc
    51121 cgtggcgcta aaacactagt tattgtctca gagggattag aaaaggaaag tgaccagctg
    51181 gtgctaccaa ctgtccaccc ttatctaact actctaacca cagtcgtacc gactcaatta
    51241 cttgcatatt acgcaacgct attacgggga ttcgacgttg acaaaccacg taatttagcg
    51301 aaatcagtga cagtagaata ataaaagaac aaggaccagt gtcagacatc tgacactggt
    51361 ccttgttctt ttatttttta tgaaaatatc tcttaatcca gtccgatttc ttctttgact
    51421 actgccgcga ttttatcgac atagtaatta actttctcat cagtaggcgc ttctgccatc
    51481 actcgaagta atggttctgt accggaagga cgaactagga ttcttccttc tgatcccatt
    51541 tcagcttctg cctcttcaat cacttttttg attgccggaa catccatcgc accgttttta
    51601 tcacttacgc ggatattcac taatttttgc ggatagattg ttacctctgc tgcaagctct
    51661 gataatttct tacctgtctg tttcatgata ttcagtaact gaattcctga aagcatccca
    51721 tctccagttg tgttaaaatc taagaaaatc atgtgcccag actgctcgcc gccaaagtta
    51781 taatcgtttt tgcgcatttc ttctacaacg taacgatcac caacttgtgt aacgacatct
    51841 ttcaaaccga ttccttccac cgctttgtgg aatcctaagt tactcatgac agtcgtaacg
    51901 atcgtatctt ttttcaatcg atttttttct gccaagtatt tagcacagat aaacatgatt
    51961 ttatcaccgt cgacaatgtt tccaagttca tccaccgcaa taatacgatc gccatcgcca
    52021 tcgaaagcta gtccagcatc tgctcctttt tcaacgacca tctcagctaa acgttctgga
    52081 tgggtagagc ctacgccgtc attgatattt aacccgtctg gatttgtgcc cattgtataa
    52141 aaatctgttt caagatcggc aaacaaacga ttaacacttg ttgaagttgc tccgtttgct
    52201 gcatcgacac aaatggtcaa accggaaagg tcaccgttga tcgtttgtac aaggaattga
    52261 gaatatttca ataagccttc tggaaattct tctacagttc ccaatccttc agctgatgga
    52321 cgaggcaatt catccacttc agcatctaat aatgcttcaa tttctagctc ttggtcatct
    52381 actagtttaa aaccatctgc accgaaaaat ttgattccat tatcttgtgc tggattatga
    52441 gaagcagaaa tcatgacccc tgcgcttgct ttttgcaaac gtgtcagata agcaacacct
    52501 ggtgtagaga tcacacctag ctggaacact tcgataccca ccgataaaag tcctgaaatc
    52561 agcgcttctt ctaacatttg acctgagatt cttgtatccc gccctactaa cacgcgtggg
    52621 cgttttcctt cttcttcatg ctggcttagg acataacctc cgcaccgtcc cagcttaaat
    52681 gcaagttctg gcgtaagttc cttgttggct tcaccgcgta cgccgtctgt tccaaaatat
    52741 ttacccatag ttgtaaaatt actcctttaa cttaattttc tgaggaattt gttctgctca
    52801 tttgtgttga ttcgcttgtt tctgatgttt cttctgtact tgtggtcgag ctgcttgtcc
    52861 cagattccga actggaagaa atggttgttg tcgatggttc cgttcctgaa tcagcagatg
    52921 aaatacttct actcgtactt gtattttctt gtgtccgact gctcgtcgaa gcagagctac
    52981 tgcttgacgt ttgcaacacg ggtgtaatct ccacactaac tgattcgggc ttgatactta
    53041 acccctcttc tactggtatt tcaattttac gagttgttcg atgattgata cctgagacat
    53101 tgatcggtac agcaatactg tcaccgatcg atgcaagcaa atcgctagag ccgcttactt
    53161 ctgcttccag ttttgaaaaa ctgaaatcat agctttctac cccggcagct ggtgttcctt
    53221 gctgtatccc ataaagtcgt accgacttgc ttggcttgat cgaatcagct ctcactgtta
    53281 cttgcactgg atcagagacg atacttaata cttcccctgc tgcattcagc gcctgtattg
    53341 ggacctcact attaatccca tcatctgtga ccatcgaagc gtccacatca gctactactc
    53401 gtttgatttc tgccaatgtt ttgtcacctg tagtgataga gactttttta ggactaacgg
    53461 aagtatcatc aagttcatat ccagatggtg tgaacgttga aggaatcaac gtttccacat
    53521 cgaaatcttt tattaccttt ttctcaaccg ttactgtaat agtcgatggt tcgatctttg
    53581 ctgtgacagc actgctcaaa ttttgaattt ttagcggaac ttcatgtgtt ccttcgccca
    53641 gcttagtgat atctgctgtg acgcggaaca tccgtgtatc ttcgtttgcc tctgcattca
    53701 actgaactcg gtttgcactg gaaagtttga cagtcactgc attttcatat ccatggatat
    53761 agtagcgctt tgaatcataa gaaatcttga tcggcacccc cgtaactgtt tgattatacg
    53821 cttcatttcc agaaagcgta tttggaacgg actggccatt tgcattgaag tacagcgcaa
    53881 tactgaacag caatgcaata aaactgtaaa gaatgcttgt ccgtttctct tttgagatca
    53941 tttgcgatcc cctcccttag tcataccgtc taggaaagat tgaatcacgt ttttcttgtt
    54001 ttttgttttc tcttcagcaa ttagttctct acgcaagatt gccaaatatt cttgctggga
    54061 taagccagcc atcagttcat tatctaacgt gatgctgacg tccccggttt cttcagagac
    54121 aacgatggta atcgcgtcac tgacttcgct tataccgaca gctgcccggt gtcgagtccc
    54181 gaattcttta gggatcaaca tactttctga tagaggaaga taggcgcttg ctactgcgat
    54241 ctttccttct tttacaataa ctgcaccgtc atgcagcggt gtattaggaa taaagatatt
    54301 gatcagcagc tccccagtga tatctgcatc taaagcaatc cctgtctcaa tatactcatc
    54361 taatccagta tgacgttcga tagtgatcaa cgcaccgatt tttcgcttgg acatatattg
    54421 gatggctttg tcaaaagaca agatcatctt ctcatcttcc tgctgttcgc tttttgattg
    54481 tttgaaaagc gaactgcgtc caagatgttc cagtcctctc cggatttccg gctgaaaaat
    54541 cacgatagca gctatcaccc cgtacgtaat gacttggttc atcagccaag acaaggtgtg
    54601 cagaccaatg atttccgcta cgattcggat aacaataaaa atagctacac ctttcagaag
    54661 ctgaatcgct tttgtgcctc gcagcagttg gatcagtttg tatacaagat accagacaac
    54721 cagaatatca agtatgttca ccaaaaaatt cagagagaac aagtcaccgg aaaacatttg
    54781 ctgccaatag tccaaattaa aaagttcccc gatttgaaat gacataaaaa gcctctcttc
    54841 tcttttgaga tttcctctct agtatatcat atgttttaat tttagaggct gatatgctcg
    54901 tatttttctt gagaaaaacg cataattttc aagaaaattg caagtatgcg ttttattttt
    54961 ctctgcaata aatattttta aacttcagag aattattcat tatatttttt ctgttatttt
    55021 tccttttttt cttttgtagc ttgttccatt tttttacgtc gttctgctct ggcagctctt
    55081 ctagcagctc ttctttcttc tgcttcaagg tccgtcgttt tttcttttct agtcttaacg
    55141 ggtacatcga tagattcctc tggttcaagt cgtttcttga ttttttctaa ttctcccgtc
    55201 agtgcagcat agttgtaaaa acgtgtttta gcgtcgtgga cagtcttttc aaataaagct
    55261 tttgcaagat ttggatttgc gttatctagc gaggaaaagc gaacttgttt cctcatgaaa
    55321 tcaggcatag cagagaaatc aggttttttg tagtccaaag tcattgggtt cttgtttttt
    55381 tcgaccattg tagggttata tcgatataat gaccaataac cagaagaaac agcttctttt
    55441 gcttctttaa gcgattcttt cattccgcca agcaagccat gtgtgataca aggcgtatag
    55501 gcaataatca gagaaggacc aggatacttt tctgcttctt caaatgcctt gatcgtttgc
    55561 atttgattgg cacctgaagc aatttgcgcc acataaacat ttccatatgt catcgccatc
    55621 attcctagat ctttctttga agcatacttt ccactagccg aaaatttagc gattgccgaa
    55681 gctggtgttg cttttgaggt ttgaccgcca gtattggaat agacttcgtt atccaaaaca
    55741 aggatattca cgtctgctcc gctggcaagt acatgatcga tcccgccaaa accaatgtcg
    55801 taagcccatc cgtctccacc aatcatccat tggctgtttt ttacgaatag atcctttctc
    55861 ttgtatatat cctccagttc agggaaaatt gatcgttcgc tttcgatcgc agcttgcagt
    55921 tttgctgctc tttgctgcgt tccttcactc tcaaacatgt gtactaacca gtcttccatc
    55981 aattctcgta gttcttctga tacttttggc aatacttctt tgattgaact tgccaaagcc
    56041 tcgcgacgag tctgtgccgc tagaagcata ccataaccaa attcagcatt atcttccaat
    56101 aaagaatttg accatgcagg cccttgacca cattcattgg tcgtataagg agaagcagga
    56161 gaagcaccgc cccaaataga agaacagcct gtcgcattcg cgatcatcat tcgatcaccg
    56221 aacatttgtg tcaataattt tacataaggt gtttcgccac agcctgagca agcgcccgag
    56281 aattcaagaa gcggttgatt gaattgtgta gacaagacag tattctttcc tttgactgga
    56341 tttgcttttt gtcttaatgt cattgcaaaa gcccaattca cagcttgttc tttctgttct
    56401 tcataaggct tcatcactaa agctttttct tttgcgggac aagcctctac acacagacca
    56461 caacctgtac agtcttccac tgaaacttgg atacggtatt ttacgccgtc tgcgcctcgc
    56521 atttctctca cgataaatcc ttctggcgct tcttccattt cctcttcatc tgctaaaaat
    56581 ggacggatgg cagcatgagg acaaacaaag gcacattcat tacacatcgt acagcgatca
    56641 ctaatccact caggtacttc taaagcgacg ccccgctttt ccatagaagc cgttcctaaa
    56701 ggcatttcgc cagttgtcat tccatttgct acaagtgtac caacagagag ttgatttcct
    56761 tcctgtgcat tcactggttc aagtatttct tgtatataac cagtgttcct tttattgatt
    56821 ttttcaggca tctccaacgt tctccaatgg tctgggatcg cgacttgatg aagcagttcg
    56881 gtcgtccgat cgattgcttg tatgtttttt tctacgatag ccattgattt atgtccatag
    56941 cttttcaggg cttcttcttt tagcagcgga agcacctcat caaaaggaat gatgtcagct
    57001 aatttaaaaa aagccgtttc cattgccgta ttgatccggc gtcccaaacc gacttcttgg
    57061 gccaatctca tggcattgat cgtatagaat ctaatgttat tttcagccaa gtaacgtttt
    57121 aattttaagg gcagatgcgt ttctaattgt tcgtcgctcc aaagagtatt caataaaaaa
    57181 atgccacctg gcttcaatcc tttgaccaag tcatagctat gcaggtaagc tggtgtgtga
    57241 caggcaacaa ggtccgcatg ctcgacaaga tacgctgaac gtatcggcgt atctccaaaa
    57301 cgaagatgag agacagtcag accgccagat tttttcgaat cataataaaa atagccttgc
    57361 gcatatttat cggtgtgatc tccaataatc ttgatagctg atttatttgc tccaactgtt
    57421 ccgtcagatc caaatcccca aaacttggcc tggaatgttt gcggttctgt caaatccaga
    57481 ctttcctttg gttccaaaga ttgataagtc acatcatcca cgatccctat ggtaaaccgt
    57541 tttttgcgta tgcttgggtc ttttttcaat tcatcaaaaa ctgctgagat ttggtcggga
    57601 gtcacatctt tagaccccaa tccgtagcga ccaccaataa cagccgggcg aagttcagaa
    57661 tcatataacg cactttgaac gtctaataag agaggttctc cacctgcccc tggttcttta
    57721 cttcgatcaa gaacagcaat tgtttttact gtatgaggca acttttgcac gaatgtttcc
    57781 actggaaaag gacgataaag atggatattc aaaaaacctg ttttccgtcc gtttgcattc
    57841 aagtaatcaa tcgtttgttc aatcgtctgt gcaactgatc ccatagaaac aatgacttct
    57901 tccgcatcgt ctgctccgta ataagtcacc aagtcataag aagttcctcg taatcggttg
    57961 atctctttca tatatttttg aacgatttca ggtaatttat cataataggg gttgatcgtt
    58021 tcccgttgtt gaaaatggat gtctggattc tggttcgtac cactaacagt cggatgattg
    58081 ggattcatcc ctcgtttacg aaatgaagct aatttctcct gattgatcag aggagctaat
    58141 tcttcatatt ccaaaacttc gattttttga atctcgtgac tcgtacggaa tccatcaaag
    58201 aaattcaaaa aagggacttc tccttcaata gaagcaagat gggcaacagg tgctaaatcc
    58261 atcacttctt gtacactgct ttcagctaac atagcaaatc ctgtttgtct tgcagccatc
    58321 acgtctccgt gatctccgaa aatattcaac gcatttgtcg tcactgcccg actagcaacg
    58381 tggaaaacac ttggcagtag ttccccagca atcttgtaca tattcggtat catcaaaagc
    58441 agcccttgtg aagcggtata tgtagttgtc agtgcaccag ttttcaatga tccatggacc
    58501 acaccagctg ctcctgcttc ggattgcatt tctactactt ttactggttg accgaatacg
    58561 ttttcttttc ctgctgctga ccattgatct actaattcag ccatggtcga actgggtgtt
    58621 attggatata ttgcagcaat ctcggtaaat gcataagaaa tatatgcggc tgccgtattg
    58681 ccatccattg ttttcatttt tcgcatgatt catcatcctt gttcatattc tctcttatta
    58741 tctgttccat tataacgtaa aacatgccat ttttttcttt tatttataaa aatattttac
    58801 atgtatctat ttgaattggt catctaattt ttagacaaac agtctaaatc gttgcctttt
    58861 tttgtcagac atgtataaac tataagcgaa agaggtgttt taacatggat atatcagtga
    58921 ttgatgcaac gaaaacaaat acacaaaaag gaatccttta tggttcatcg aatgctccta
    58981 aaaaaatggt cgaattcatc aacttagctt gtccgtattg ccgacaatgg tttgaagaat
    59041 cttatgagct attagaagaa gcagtccaaa gcgggcaatt gcagcgagtg atcaaattgt
    59101 tcgataaaga aaaagaaagc ttacagcgag ggaacgtgat gcaccggtat ctcacaatca
    59161 gcgatggcca aaaagcgatc aaagagatca aacaaatatt cgatactcaa gacgagtgga
    59221 aacatctttc tctccaagaa gtagcggatt ttgcagtaga taaactaaag ctgacagaac
    59281 aaaaagacga acagctgagc caagctgtca tcaacgaagc tgagcaagca catattcgat
    59341 ttgttccaac tgtcatcttg ggtaaagaaa tctttgatga atctatttca atagaggaat
    59401 taaaagaatt gatccaaaca aaataaaaaa ataaggagcg tgggacaaaa gtaaagaaca
    59461 cttttgtctc acgctctaaa cacgtataaa cggcggaagc agaagcaact ccttcttatt
    59521 tcttggagtt aaacgcttct gtcccagcct ccttattccg aataaatggt gtccaaaagc
    59581 ttgcatctgc cagattatct catttcttat gacaagctaa gcaaattatc tccaccaaat
    59641 aatttattca tatttttgac ttgcgcatac taactaattc ttaaattttc atacaaaatt
    59701 tctcaatttc ccttaacgag taatatttat gtacggtgat tctcactagt atcccaatca
    59761 ctttttaagt tatcccgatt tcaattctac ttcaaaaata gttgctttta tgtatgctgt
    59821 tagttgatta gatggtcatt tgttgagcta ggtcgtttat tttaaacaag ttgtttcttt
    59881 agcttatacc tatctcgtct accaaacgca agatggcaaa cacaaatgca tacgcatctg
    59941 tatttgccac tttgattcgt ataaagctaa caactattaa ctcctctcag ctaatagttg
    60001 ctctcttttt tattcagatt catttttgct gatcacagcc tagtatttca atggttttga
    60061 acactaaacc aaattgttcc attacaacat tcactggctg atgaaatgga ttagtagatt
    60121 tcgattattc aaagcgattt tctattggtg gatcgtgttc ccaactaata agacggattc
    60181 caaacctatc gtacagatct tctaaaccac ttataaattt tgcacctatt tcaggtgttt
    60241 tattgcgtgg atattctatt gttacgccat taaatggata ccaattagga ttaggctctc
    60301 tttttttctg tacatctccc atagaatagt tagcactttc tgggacataa tatattcttc
    60361 gaccatcctc taattcatct aataagatat ttcgtgatcg tctatcactt gctaaatgta
    60421 gacgccacgt gcgataccat gccatgctac ctgcatctgc agaaaatcca tcaaataatg
    60481 caacaattgt atcttctatc acaatccttt ccccatctac ttcatattct aatctaaaat
    60541 caaactcccc tcgggtaatt tctggtcttg gtggcttagg acccaatatt attctactta
    60601 aaaccgcatt cgttcccaga tgaaacacag ctataacaac aataatgatt aatattttgt
    60661 ttctatttga taaatttctc attattcgca catcacctct acattctcac atattagttc
    60721 gtaatactcc tttaatatca aaatatctac tggtcgtaat aatattcgct atgaattcct
    60781 actactcgta taagaaaggc caaatcatca gcttttactt gctgatgatt tggcttgttt
    60841 ctatttatcg taattgtttt ctaatcacca aaattgttaa caatcggttc agatatttcc
    60901 cagttgatta aggtaattcc gtattgtgtc aaaacttctt gcgcttctcc gaaattaagt
    60961 acagttctta tgtatccact ttctcctaat ataaagtcta cctctctttt aaaaccttcc
    61021 tcgagatttc taagggctat atgtggtttt aatgacattg attgtacatt ttcccccata
    61081 taatattgcg cacttcctcg actaaaatag attcctagat cgtccccaaa aggtagatct
    61141 tctctaccac tagctaatct acttctccat cttcgttctc ttttcataga acctgcgctg
    61201 aactcacacc cgttaaactc agctatgaca gtatcttcaa ttatgttaat ctgtccatct
    61261 agttcgtatt caatcctaaa tggaaattct ccataggtga tttccggttc gggtatttta
    61321 ggtgagatta atgcaaagaa aggaaatatt aagcttggca acatcaaaaa aactcctcct
    61381 atgatcacaa ttcccgatgt aatccaaaat ataatttgta caattctcat tgatccattg
    61441 tattccattt tattccccct attactattc atatccgcct atctatatcg atcttccaat
    61501 gcagagataa ttgcttccat accttgattt tttactgagt tacctatgcg agatatcatt
    61561 ctccactaag gtgcatcata ttgtctatct aacatgatta attctccaat aggtgtatac
    61621 tcctccaaaa acaacattta atgcatctcc ctcgacaaca taagctacca tgtttgctgt
    61681 atatgtgttt gagtctaatt cataagcaga taaatttact gtcgctggat taaatataat
    61741 cgcattcgta tttatagcta ctgaatttgc tgcttcagca ccaccttttg aatgtccgat
    61801 cattgtgatt tcaatagtag gatttacgtg gacagccctc accgtttacc ctgaccatta
    61861 tagaaatcgc ttactttttt gaactacgca acaaaggata caatgctcct aacttcactg
    61921 tctccatttt tgtccactaa ggaatataaa aaataggcat taattcattt aattcggtca
    61981 caacacgtta ccttttaaat cattatactg tatctcatac attattatca ataaactatt
    62041 ataaaaatgc aaaaaatgct ttatcaatct attttgtaat tggtcataat atcattgaaa
    62101 tcataccctg tatcgatttt tcctatgaat ctcgtctatt tgtgattgac gaaaaagagg
    62161 caagacagac actggaatgg taattttacc cactattttg ctccaactat ttgtaatcgt
    62221 acccactttt gaaataccga gatgatcatt ttaattgata tgctgattcc caagcaaaac
    62281 cccaagaaat taaatgctct atcgcatcta gcttcttggg ttttgcttat ataaattcca
    62341 acatttaaat tttttatgct gttcggtctc agaaatgccg tttttgctta tatcacaata
    62401 tcaagatgtc aaattattcg tgttgccgat gactttgatt aatttttttc aaattgttta
    62461 ttaagactaa tgctgtcttt ttgcagatac caatgttcga actttttaac attatcatat
    62521 gatcctgcaa gttttaactt cttaccatta gatgttatca aagttataat taaccttgcc
    62581 tttttcccat taggatcttt tattttatga attttctgca ggtaatctct cctaatggca
    62641 gctattttta cctcatcagt gctgataaac cattgatcat ccacatacat actgcctgtt
    62701 atgttgtgtt tttccatttc ttccgagaac gaaaagttta aggctcgttc ttacttccaa
    62761 agttgaatga gtccatggat tggacttagt gtgagaattg agaacaacaa taagaatcca
    62821 tatcctcccg caaacatccc taaggctata ctgtctctcc atccataagt aggattagaa
    62881 gagaaaagag tcaccgtttg taataatatg aaaaggaata atccagcaca aacaccaaag
    62941 ataacaactc caaacattga atttttatac aatgaccatt tatttatagg cttttctttc
    63001 attttacttc cccctactgt tctgttcaca cggaatgaat tcaaagttca taagccctcc
    63061 tctttgaaca tatgaatacg acttgctaac tatactttaa aaaagcttgt tttaagcggt
    63121 tataggcgag ttgtaatctc tattcatctc attagcaaat aacttgccga aaagtctact
    63181 gaaattaaag cagatagtgg aagaattatt tagccaggat agacactatt tgtgaaacac
    63241 atgtctttac ttttgaaatt ctatgatgaa aagccttgga atacagtagc tcttaattgg
    63301 taatactttt tccattataa ataacctctt ttcattttat atttatcccc tctaaaaata
    63361 atatctaatc taatatttta aacttatatt tccctcgctg tgctaataga gcattactaa
    63421 ataaatagaa actagaaaaa agcattacgc ctgccagaat aaaccaatta tttgt
//
LOCUS       DAJSBL010000010        62714 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.010, whole genome shotgun sequence.
ACCESSION   DAJSBL010000010 DAJSBL010000000
VERSION     DAJSBL010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 62714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 62714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..62714
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.010"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(26..1522)
                     /gene="lysS"
                     /locus_tag="ONT85_000753"
     CDS             complement(26..1522)
                     /gene="lysS"
                     /locus_tag="ONT85_000753"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HCR2377748.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKAGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="ONT85_000754"
     CDS             complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="ONT85_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HCR2377749.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="ONT85_000755"
     CDS             complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="ONT85_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HCR2377750.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(3742..5853)
                     /gene="ftsH"
                     /locus_tag="ONT85_000756"
     CDS             complement(3742..5853)
                     /gene="ftsH"
                     /locus_tag="ONT85_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HCR2377751.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="ONT85_000757"
     CDS             complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="ONT85_000757"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HCR2377752.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="ONT85_000758"
     CDS             complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="ONT85_000758"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HCR2377753.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8053..8523)
                     /locus_tag="ONT85_000759"
     CDS             complement(8053..8523)
                     /locus_tag="ONT85_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HCR2377754.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8569..8994)
                     /locus_tag="ONT85_000760"
     CDS             complement(8569..8994)
                     /locus_tag="ONT85_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HCR2377755.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9061..9330)
                     /locus_tag="ONT85_000761"
     CDS             complement(9061..9330)
                     /locus_tag="ONT85_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HCR2377756.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9341..10936)
                     /locus_tag="ONT85_000762"
     CDS             complement(9341..10936)
                     /locus_tag="ONT85_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HCR2377757.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(10951..14472)
                     /gene="mfd"
                     /locus_tag="ONT85_000763"
     CDS             complement(10951..14472)
                     /gene="mfd"
                     /locus_tag="ONT85_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HCR2377758.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14489..15049)
                     /locus_tag="ONT85_000764"
     CDS             complement(14489..15049)
                     /locus_tag="ONT85_000764"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HCR2377759.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15402..16376
                     /locus_tag="ONT85_000765"
     CDS             15402..16376
                     /locus_tag="ONT85_000765"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HCR2377760.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            16546..17946
                     /locus_tag="ONT85_000766"
     CDS             16546..17946
                     /locus_tag="ONT85_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase/peptidoglycan binding
                     protein"
                     /protein_id="HCR2377761.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18024..18746)
                     /locus_tag="ONT85_000767"
     CDS             complement(18024..18746)
                     /locus_tag="ONT85_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR2377762.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(18825..19331)
                     /locus_tag="ONT85_000768"
     CDS             complement(18825..19331)
                     /locus_tag="ONT85_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HCR2377763.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSINHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19454..21253
                     /locus_tag="ONT85_000769"
     CDS             19454..21253
                     /locus_tag="ONT85_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HCR2377764.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            complement(21321..22895)
                     /locus_tag="ONT85_000770"
     CDS             complement(21321..22895)
                     /locus_tag="ONT85_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HCR2377765.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
     gene            complement(23150..23659)
                     /gene="mreD"
                     /locus_tag="ONT85_000771"
     CDS             complement(23150..23659)
                     /gene="mreD"
                     /locus_tag="ONT85_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HCR2377766.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            complement(23663..24517)
                     /gene="mreC"
                     /locus_tag="ONT85_000772"
     CDS             complement(23663..24517)
                     /gene="mreC"
                     /locus_tag="ONT85_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HCR2377767.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            complement(24672..25310)
                     /locus_tag="ONT85_000773"
     CDS             complement(24672..25310)
                     /locus_tag="ONT85_000773"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HCR2377768.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            complement(25311..25961)
                     /locus_tag="ONT85_000774"
     CDS             complement(25311..25961)
                     /locus_tag="ONT85_000774"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HCR2377769.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGRFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            complement(25974..26873)
                     /gene="rsgA"
                     /locus_tag="ONT85_000775"
     CDS             complement(25974..26873)
                     /gene="rsgA"
                     /locus_tag="ONT85_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HCR2377770.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            complement(27038..29107)
                     /gene="pknB"
                     /locus_tag="ONT85_000776"
     CDS             complement(27038..29107)
                     /gene="pknB"
                     /locus_tag="ONT85_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HCR2377771.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            complement(29104..29844)
                     /locus_tag="ONT85_000777"
     CDS             complement(29104..29844)
                     /locus_tag="ONT85_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HCR2377772.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            complement(29857..31215)
                     /gene="rsmB"
                     /locus_tag="ONT85_000778"
     CDS             complement(29857..31215)
                     /gene="rsmB"
                     /locus_tag="ONT85_000778"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HCR2377773.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKMLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            complement(31215..32153)
                     /locus_tag="ONT85_000779"
     CDS             complement(31215..32153)
                     /locus_tag="ONT85_000779"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HCR2377774.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKKAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            complement(32146..32637)
                     /gene="def"
                     /locus_tag="ONT85_000780"
     CDS             complement(32146..32637)
                     /gene="def"
                     /locus_tag="ONT85_000780"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HCR2377775.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            complement(32652..35060)
                     /gene="priA"
                     /locus_tag="ONT85_000781"
     CDS             complement(32652..35060)
                     /gene="priA"
                     /locus_tag="ONT85_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HCR2377776.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            complement(35313..35618)
                     /locus_tag="ONT85_000782"
     CDS             complement(35313..35618)
                     /locus_tag="ONT85_000782"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HCR2377777.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            complement(35623..36237)
                     /gene="gmk"
                     /locus_tag="ONT85_000783"
     CDS             complement(35623..36237)
                     /gene="gmk"
                     /locus_tag="ONT85_000783"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HCR2377778.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYVNKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEKRMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            complement(36724..37008)
                     /locus_tag="ONT85_000784"
     CDS             complement(36724..37008)
                     /locus_tag="ONT85_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2377779.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            complement(37026..37916)
                     /locus_tag="ONT85_000785"
     CDS             complement(37026..37916)
                     /locus_tag="ONT85_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HCR2377780.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFVLDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            complement(37936..38154)
                     /locus_tag="ONT85_000786"
     CDS             complement(37936..38154)
                     /locus_tag="ONT85_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HCR2377781.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            complement(38124..39824)
                     /locus_tag="ONT85_000787"
     CDS             complement(38124..39824)
                     /locus_tag="ONT85_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HCR2377782.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            40091..40864
                     /locus_tag="ONT85_000788"
     CDS             40091..40864
                     /locus_tag="ONT85_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2377783.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            complement(40945..41514)
                     /gene="thiT"
                     /locus_tag="ONT85_000789"
     CDS             complement(40945..41514)
                     /gene="thiT"
                     /locus_tag="ONT85_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HCR2377784.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     regulatory      complement(41580..41682)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(41688..42659)
                     /locus_tag="ONT85_000790"
     CDS             complement(41688..42659)
                     /locus_tag="ONT85_000790"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HCR2377785.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     gene            complement(42753..44126)
                     /gene="glmU"
                     /locus_tag="ONT85_000791"
     CDS             complement(42753..44126)
                     /gene="glmU"
                     /locus_tag="ONT85_000791"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HCR2377786.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            complement(44629..45447)
                     /gene="purR"
                     /locus_tag="ONT85_000792"
     CDS             complement(44629..45447)
                     /gene="purR"
                     /locus_tag="ONT85_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HCR2377787.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            complement(45614..46438)
                     /locus_tag="ONT85_000793"
     CDS             complement(45614..46438)
                     /locus_tag="ONT85_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HCR2377788.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(46411..47091)
                     /locus_tag="ONT85_000794"
     CDS             complement(46411..47091)
                     /locus_tag="ONT85_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HCR2377789.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(47114..48052)
                     /locus_tag="ONT85_000795"
     CDS             complement(47114..48052)
                     /locus_tag="ONT85_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HCR2377790.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(48297..49160)
                     /locus_tag="ONT85_000796"
     CDS             complement(48297..49160)
                     /locus_tag="ONT85_000796"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HCR2377791.1"
                     /translation="MGERMELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADH
                     LFFEEITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAG
                     LGGGSTDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQ
                     VLPSMPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMG
                     NSLEDITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGF
                     CEEVYLVRTLQ"
     gene            complement(49302..51875)
                     /gene="mprF"
                     /locus_tag="ONT85_000797"
     CDS             complement(49302..51875)
                     /gene="mprF"
                     /locus_tag="ONT85_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HCR2377792.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLS
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            52016..52822
                     /gene="proC"
                     /locus_tag="ONT85_000798"
     CDS             52016..52822
                     /gene="proC"
                     /locus_tag="ONT85_000798"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HCR2377793.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLIDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(52901..54457)
                     /gene="rny"
                     /locus_tag="ONT85_000799"
     CDS             complement(52901..54457)
                     /gene="rny"
                     /locus_tag="ONT85_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HCR2377794.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(54868..55917)
                     /gene="recA"
                     /locus_tag="ONT85_000800"
     CDS             complement(54868..55917)
                     /gene="recA"
                     /locus_tag="ONT85_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HCR2377795.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(56026..57267)
                     /locus_tag="ONT85_000801"
     CDS             complement(56026..57267)
                     /locus_tag="ONT85_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HCR2377796.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(57507..58085)
                     /gene="pgsA"
                     /locus_tag="ONT85_000802"
     CDS             complement(57507..58085)
                     /gene="pgsA"
                     /locus_tag="ONT85_000802"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HCR2377797.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(58294..59217)
                     /locus_tag="ONT85_000803"
     CDS             complement(58294..59217)
                     /locus_tag="ONT85_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR2377798.1"
                     /translation="MLRRIKQTGEDERVAFETIGEKLRQARLNKKISLDELQQITKIQ
                     KRYLEAIDNDDFDQLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQ
                     PETVSTSRKAMHQEESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDT
                     ASSLKVEGSVEESSSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITD
                     ATKPVTLEFTAVNRVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNA
                     TIKANGESVPVNPGENNQSPKNVNLTLQYAE"
     gene            complement(59956..61242)
                     /locus_tag="ONT85_000804"
     CDS             complement(59956..61242)
                     /locus_tag="ONT85_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HCR2377799.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(61235..62503)
                     /locus_tag="ONT85_000805"
     CDS             complement(61235..62503)
                     /locus_tag="ONT85_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HCR2377800.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYSDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
BASE COUNT        17307 a        13635 c        11225 g        20547 t
ORIGIN      
        1 aattccttag acagctttta ttcgattatc tcattgttgg gaatagcaaa acatcccgaa
       61 ttgattgtgc atcagttagc aacattacta atctgtcgat cccgatgcct agaccaccag
      121 ttggcggcat accgtattct aaggcttcca agaagtcttc gtctacaccg tgagcttcgt
      181 catttcctag ttcacgttct ttttcttgcg cttcgaatct ttctctttga tcaattggat
      241 cgttcagttc tgtaaaggca ttagcaaatt cgcgccccac tatgaatagt tcgaatcgat
      301 ctgtgaatcg aggatcttct ggatttttct tcgctaaagg cgaaacttcg actggatgtc
      361 cgtatacaaa cgtcggttgt gtcaatgttt cttcaacaaa tgtttcaaag aattcattga
      421 taacatgtcc gacacccata gcttcggtta cttctacatt gtgtttttcg gctaaagcta
      481 atgcttcttc caatgtcatt tcttgccaga agtctacacc agtttgttct ttgattgcat
      541 ctaccatatg gatgcgttta aagtcacttt ctaagtcgac agcttgtcca tcatatgtga
      601 tttttgctgt tcctagtact ttttcagctg cgttacggat aataccttct gtcagattca
      661 ttacatcatt gaaatcagta tatgctgtgt atgcttctaa catcgtaaat tctggattat
      721 gtgtagtatc aattccttca ttacggaaaa cgcggccgat ttcataaact ttttccattc
      781 cgccaacaat tagtcgtttt aaatgaagtt ctagcgcaat acgcaaatat aaatcaatgt
      841 ccagtgcatt atgatgcgtg ataaatgggc gtgcggcagc tccacctgcc tcattgtgca
      901 atactggtgt ttctacttca atatagccat tgccatctaa atagcgacgg atttcgctga
      961 taatttggct tcgtttcata aatcggtcaa agctttcgcg attactgatt aaatccaagt
     1021 agcgttgtct gtagcgttgt tcaatattcg ttaagccatg gtatttatct ggcagtggac
     1081 gaagtgcttt tgacaaaatc gtgatttctg aagctttgat agaaacttcg cctgtgtctg
     1141 ttttcatgat ttgtccagtt acaccaaaga aatctccaag gtctgcatgt ttgaatactt
     1201 cataggcctc atcacctact tggtcttttc ttacataaat ttgaatttgg ccttcacgat
     1261 cttgtagatg cgcaaatcca gctttccctt ttcctcggcg agtcatcata cgaccagcta
     1321 cactagcggt caatcccatt tctgctagtt cttcttttgt tcttggatca aatagttcat
     1381 gcaattcttc tgagttgtgc gtgcgttcaa atcgttttcc aaatggatcg attccatctt
     1441 cacgcaattg ttccattttt tgacgacgta caagcatttg atcatttaaa tcttctactt
     1501 gatgttgttg ttcctgtgtc acaaaagttc ctccattctc atgttaatct ctttcctata
     1561 atgccaatga aacacaaaaa aagcaagccg aacttggaat ttcggcttga aaattcacga
     1621 aaaacttaga aatcacaacg tttgtttcaa tagtactttt tccgcttttt ctacaaattg
     1681 atccagcagg tcattgattt cttgctgttt ttctgtctga ttgattgcta ctttcacttt
     1741 cgctgctcga ggaatccctt ttaaataata agccgcatgt gttctgaatt ctctacaagc
     1801 tactttttct cctttgagat ctgctaagcg ttgtaaatga agtttagctg tagcaatttt
     1861 ttcggctggt gttggttcag gaatcagttc ccctgtttcg agataatgct gtgtgcggtg
     1921 aatcatccat ggattgccca gcgctgcacg accaatcatc acaccgtctg caccgacata
     1981 gtcaagcatc cattttgcat cttctggcgt tttgacatcc ccgttcccca taaatggaat
     2041 cgtcaaatgt tttttcactt ctttcaatac ttcccagtcc gcttttcctt catacatctg
     2101 aacccgtgta cgtccatgca tagcgatggc acttgctcca gctgattgtg ctgctaatgc
     2161 attttctaca gcaaagacgt gctcttcatc ccaaccaata cgcatcttga ttgttactgg
     2221 gatatttact gctgaagaaa cagcttggac catttcgtat actttctcag gatctaaaag
     2281 ccattttgct cctgcttctg ctttgattac tttatttact ggacagccca tattgatatc
     2341 tattatatcg gcagctgtgt tttcttcgac aaattgagca gcttcaacta gactatcttt
     2401 atttccgcca aatatctgta aacttaatgg atgttctgtt tcttcaatgt ggagcatctc
     2461 taaagttttt ttgtttctga aatgaatacc ttggtcactg atcatttcac atacgactaa
     2521 gcccgcacca aattctttga cagtgactcg aaatgctgaa tttgttattc cagccatcgg
     2581 tgcaacaacg acacgattag ggatttccac attgccaatt ttccatgtgt gttccactta
     2641 aatccctcct aaggcttctt tcttcaatcc atctaattct tcttcgctat acttgtattt
     2701 gttattacag aaagaacaaa ccgcttctgc accgtgatct tcgtctatca ttgcttggat
     2761 ttcttctgca cctaacgcga taattgcttc tgcaaatttt tcttttgaac agtcacattt
     2821 gaattgaaca ggcatttttt ccaaaatgtt caattcttct gtaccaagca atcctttcaa
     2881 tatttcttct ggtgtctgtc cttcatctaa gagggtagag attcttggtg tcgtttttaa
     2941 ttgttcttcg atcttgctga ttgtttcttc atcggcacct ggcatgattt ggatcatgaa
     3001 tcctcccgcc gtcttgatgc tgtcgtcagt attgaccaat acactcagtc caacagctga
     3061 agggatttgt tctgagatag caagatagta agtaaaatct tcagcgattt ctccagaaac
     3121 aatcggcgtt tgcccagaaa aaggttcttt taatcctaag tcttttgtga cagtaaacat
     3181 tccttgtgtt ccaactgctc cccgcacatc gattttacct tcactattta gaggaagact
     3241 gagatgcgga tttttgatgt aacctttcac atctcccttt ccatttgcat ctactacaat
     3301 tcctgttgct ggaccatcgc cttggattcg aacagttagt ttgtcttctc ctttgagcgt
     3361 cgcaccaagt agcagactac cgaccaatgt ccgtcccaaa gcagctgagg aggtgtgcca
     3421 agtatcatga cgtctttgtg cctccgctac ggtatttgtt gcttgaacgg cgtatgctcg
     3481 gacgaatccg tcgtgagcaa gtgccttaac caaataatct ttcatttgtt atgtactcct
     3541 ttttccgttt ttaaactcat agtgaatcat actagcaact actgcttttt tcaactttgg
     3601 aatgcttctt ttaaaaataa ttcagaatca tctagaaaac aggacatcac cctatttaca
     3661 acttaaataa agaaatctcc cgaaaaccgg tttttgttcc ggttttcggg agtcatatca
     3721 gatcaattga cctatatttt tttatttgta ttgatcgtct gaatgattat cttctgattg
     3781 ttgagaatct gaatcatgtt ctgctttatt ttcctcattc agttcatgtt ttgcttcttc
     3841 aaaatcacgt ttttcttccg cttgtttttc agcgtctttt tcttccaaag cacgttttgc
     3901 ttcttcaaaa gttttcgctt ctttttcgct tggataatca tttgactcag taccttcagg
     3961 catcacaccg tgttcaaaca atgacttgat tgctttggca tccaatgttt cgaattccaa
     4021 aagtttttca gcaatcagtt tatgctgtgc acgatgagct tcgatgattt ctcgagcttt
     4081 atcatgagct tccatcaaga tttttcgtac ttcttgatca atctcgaaag cgacttgttc
     4141 cgagtaagct tttgtttgac catagtcgcg gccaacaaac acttgatggt ttccttcata
     4201 ttgaacagga cctaaacgat cactcattcc atattctgtt accatgcttc gtgctagagc
     4261 tgtcgcttgc tcaaagtcat tggaagcacc agttgattgg acattgaaga tgatttcttc
     4321 agctgtacgt ccaccaagta agcctacgat ttgttcaaac atatcttctt tcgtcatcaa
     4381 gaactgatct tcttttggta aagcgatcat atatccgccg gcgcgtccgc gagggatgat
     4441 cgttactttg tggacgaccc gagcgcgact caatactaat ccgacaatcg tatgaccagc
     4501 ttcgtgataa gcaaccattt cgcgttcttt tttgctgatt acgcgatctt ttttagctgg
     4561 acctgcaatc acgcgatctt ccgcttcatc gatatcagaa gcatcgattt tctttttatt
     4621 tcttctagca gctactaacg ctgcttcatt caagacattt tctaaatctg ctccggcaaa
     4681 acctggtgtc tgttgagcca caactttcaa gtcaacgtca tctgccatcg gtttattccg
     4741 tgcatggact cgcaagattg cttcacgacc ttttacatct ggacgtccaa ctaaaatctg
     4801 gcggtcaaaa cggcctggac gtagtaacgc tgggtctagt acatcagaac ggttggttgc
     4861 agcaatgacg attactcctt catttccgtc gaagccatcc atttctacaa gcaattggtt
     4921 caatgtttgt tcgcgttcat catgtccgcc gcccataccg gcaccacgtt gacgacctac
     4981 cgcatcaatt tcatcaatga aaatgatagc tggtgcgttt ttcttcgctg tttcaaacaa
     5041 atcacgaaca cgacttgctc caacaccgac aaacatttct acgaaatcag aacccgagat
     5101 cgagtaaaac ggtactccag cttctccggc aacagctttt gctaataagg ttttacctgt
     5161 tcctggaggt ccttctaaaa gtacacctgc cgggatacgg gcacctaatt caacaaatcg
     5221 gcgaggatct ttcagaaatt caacaacttc aactagttct tgtttttctt cttccgcacc
     5281 cgctacatca gaaaaacgta cgcgattagc ttttttgtct gcttctttgg cttttgattt
     5341 accaaagttc atgacacggc cgccaccgcc accgccgccg ccttgttgtc ccatcatcat
     5401 gtaaaagaaa aagatgatga ttacgattgg caagaagcta agcaataggg aaacccatat
     5461 accgctgctt gattcttctt ttactgttgt agcaactttg ttttcttttg ccagttctgt
     5521 tacttggctc aatgttgtat catttggcaa aatgattgtt gtgaaatggg tagtagtcgt
     5581 atctgttgaa cctaagatag ctaacccacc gctgttagcg acggtctgct gttctttgta
     5641 ttcaccagtg atcttatata caccatttgc aggctgcact tcaaattctt tgatcttgcc
     5701 tgcttgtagt tgttctgtga attttgagta ctcgatatct gacgatcgct ggccgttatt
     5761 accaaaaatg aaataaacga ccattaccat cgctaagatt acgaggacat aatacaaggc
     5821 atttttcatc atgccgttgt tctttttgtt catagttgcc ctccctaagc gtcaatttaa
     5881 tttaacagtt ttttatcaat cctgaccatt ctattgtacc ataatttaat tgagatacag
     5941 cattaatttg attgataaac actaggtttc aatacaccga tgtacggaag gttacgatat
     6001 gcttctgcat aatccaagcc ataaccgacc acaaattcat ttggtacgtt gaagccaaca
     6061 taatctgctt cgatatttac tactcttcct tcaggtttgt caagtagtgt aacaattttg
     6121 actgattttg ctttgcggta tttgaaaaga tccactaaat aagcaagtgt tcttccgctg
     6181 tcaatgatat cttcaacgat caacagatcg cgaccttcta cattcgtatc taagtctttg
     6241 acgattttta cttcccctga cgagactgtt gcattcccgt agcttgaaac atccatgaaa
     6301 tccaattcca aatacgtatc gatagaacga acgatatctg ccatgaatgg taccgctcct
     6361 tttaagacac ctactactag gggattggtg ttttggtaaa tttctgttaa ctctttgcct
     6421 agttctttgc aacgaacttg tatctcttct tgtgagatca aaattctctc aatatccttt
     6481 tccaacatca ggttctcctt ccaattgctt ctttttgata tttgtaaaga agtatgtagt
     6541 gtattttatc agtttcttca cgaatactca aatatgaaaa ggcaaaaggt atcaagctat
     6601 atatatttct tttggcatct gtaatgatcc aagcatgctg cctttgtgaa ttaggtactt
     6661 tctgatcgat aaaatatcta gatacttttt ttgtgagatt ctctgttaat ttaatgcggt
     6721 ctcctgcttt tcttttttcg acatacagtt gcacatcagc aggaatccaa atgtcatgag
     6781 aaaatagaga ccatgaatct gttgctttga tgtcccctgc attttcaggt ttgaccagac
     6841 caatccattc tgtttcattc aaatatattc cttgttctgg aatcagcaac aagcgctggt
     6901 cttctgtctg gctagttgct tgtttttctt ttttagagag tacaacaaaa tgatactctc
     6961 ttttcagtat ccaattattg ccaatactga gttgccattg gctttttttc tgttgccact
     7021 ggtctaaaat cgactccatt tgttgttctt tcaaaataat tcctgttttt gagaatgctt
     7081 tgtcgaaaaa ggagtaaaaa aaatagtatc gttcagcatc tgtcatttct tcaatcattt
     7141 ctgcagaaaa tcgaaattgt tcctcccact gttcgacttc cttttcgatc aactgtccca
     7201 tatatttttg aatgacttgg tctgcccatt ggatctgttg cgaaaattgc tggaaatgtc
     7261 tcaacgcctg tgtattttct tgtttcaatt gaggaactac caaatgacga agccgatttc
     7321 tctggatatc taacaattgg tttgtctgat cctcaaagta aacaagctgc gattccgctg
     7381 cataagtata taggtcttct ttgctaaaac tcagcaacgg acgaatcagt ctgcccgtag
     7441 caaatggttg tacttctttg attccagaaa aagtatttaa ctgtccccca cgaacgattt
     7501 tcattaacac cgtctcgatt tgatcatctc cgtgatgagc agtcatcaac gtgtcatatt
     7561 gtccttcttc catcacgcga gtgaattctt gataacgaaa gttacgagct tctgtttcta
     7621 tggcggattt ggctggatct tcccaaatac ttacatataa agggagattc ttctcttcac
     7681 aatatgagcg aagataggca gcttcttctg ctgattcttc tcttaattgg tgattgatgt
     7741 ggacgatacc taaagaaaaa ccgatttggc gttgcacttg taaaagtaaa tggagcaaaa
     7801 ccatcgaatc aacaccacct gataccgctg ctaaaattct cgatccttgt tgccagaatc
     7861 cttgttcatt cccgtgccga ataaatgact gctgtaatag cggtgctttt ttcccgcctt
     7921 tttcaacgtt catcctttcc acaccctttc ttcctaaaaa aaagagagcg ggaaaacgtc
     7981 taaaacgaca attaacctgc cctctgttga tactatgacc caaatagtcg cactatcatt
     8041 atttgcggct ttttagctgc gacgtccgcc acggccaccg cgctttcctt ctgtgtttcg
     8101 cttcaatgaa gataagcggt catcgctatc tttcaaaaat gaactcatca atgaatcgaa
     8161 gtcttctttt tgagctgaag gttgactgcg agaaaaagat tttttctgtg ctggacgatt
     8221 tcggttgtca cggggatctt tttgttgata ttttttagga cggctttctt ctccagattc
     8281 agcaggttgt tcctgcgctt tccggataga tagtccgatt tttccatcat catttactga
     8341 aataacttta actgttactt catctcctac acttaaaaca tcatggatgt cttttacata
     8401 gccatttgac acttcgctaa tatgaactag acccgttttt ccagcaccta gatcaataaa
     8461 cgcaccaaaa tttgtgattc ctgatacttt tccttgcagt tttgctccta cctcgattga
     8521 cataaaaaaa atgttcctcc taattatttc ccttttttat attcctgctt attttgcagt
     8581 tgtagaacta gttgattgat ctgtttcatt tgtcgagctg tctgtatttg attctaatat
     8641 aggataaact ttttcaccgt cttttgagta aaagaatttg cttctggcaa gtttagctac
     8701 atactcatca tctttcagca atgaaacatc ttggtgcagc tgatcaacgt ctgcgttgac
     8761 ttctttcatt tcagtatttg cttcgactct caattcgttc aattgatgca tcctttgcat
     8821 gtcatggaaa atctgcacac ctattatcgc gaaaatgacc aaagcaccaa caaaaattgc
     8881 agccagtctg cgtcttcgaa aaatcagttg acgttgttgt tgctgaaatt cagcatactt
     8941 tttctttgca tattccgtat ctagcgcagc gattttattg ctttttttcg tcacttggat
     9001 tcgctccaat ctttttgatt gtctttttca ttataccaat atcgtgaaag cttgtctaat
     9061 ctatttctct agtgtttctg tgcgattttc agaaataatt tcgtacattt tttgtgcatc
     9121 ttcttttttg gttgattcat gtaattccgt aattttcact tccaacgttt tgttgccaaa
     9181 ctggactttc aaaatatcgc ctactttgac atcactggaa gatttcgcca atttaccgtt
     9241 gatttgaatt cttcctttat cagctacttc ttttgctact gatcgtcttt tgatgattcg
     9301 ggaaattttt aaaaatttat ctaatcgcat atcttattcc tcacttttta atttttttat
     9361 ttaatcgcag gattttctta ccaaaaggca acattagcca ttctcgtact gtaaacagac
     9421 gaagacgaat cactgcataa atgaacacag aagccccgat cccaactcct atcacgctga
     9481 caaaaaaagc agtggaacga tgagttacag gaccaaatac tatattcaat acaccataat
     9541 acagcagcag tactactgtc atacaaagaa ggctgaacat caacttccgt ccaaaatgcc
     9601 gttctttcca aaaagcattt agctcttttg tttcaattcg aacaaaatac caaagcgtca
     9661 cacctaatcc taaaagggta gacaggcttg cacctgctgt tcctaataaa ccggtcagaa
     9721 aagaagtagt caatcctttt accaatagtc cccatcctgc gccttttaaa gaagggcgaa
     9781 acgagttctt gctttgagca atactttgat aagcttggat gactgcagtg aaagcaatgg
     9841 aaaaaacaaa taatacaagc gctgcatttc ccgcataatc tttaaacaac gcataattga
     9901 tataagggag cagcaaggct aatcccaaag acgcagctaa tgctaaagcg gtcgtcaatc
     9961 taagatagat tttggctgtc tgtaaaaact gacgatagat ccggttggtc aaatgacgag
    10021 tcaacgcagg caggaatgtc gcacttaatg cagtggcaat cactaaacct aattgtacta
    10081 aaggctgacc tcgatcatag accccttttg ctatcttcgc accgtattct gacagtccgt
    10141 aaacttccaa agcatttttt acaaagaaag agtcgatcag ctggaaaaag ataagcaatc
    10201 cactataaat cgaaaccagt ccgccttcta ccagaaaacg ccggatcaag ggctttgacg
    10261 gctgtttgaa caatggaaac cgtcggaagc ttaacgctcc tccatgaatc ttttgttcat
    10321 agtagtgaag gatcccatat gcgcataaac cgccaaaaac agctccactc attgctgccg
    10381 tccctgtttg gtacaccgtc catccaagct ttctaaaagc aaacgcacta cagacaatca
    10441 caaaaacacg aacgaactgc tcaactactt gagaaacagc agttggttcc atcaagaatc
    10501 ttccttggaa attccctcga taaaaagata gaccaggcat cagcaaaaag gtaaaggaaa
    10561 ctacttggat cagcggtttc aattgtatgt ctcccatcat ccaagcaatc cagctgctaa
    10621 atgcaaagac aaacaaccac aaaaagacac ctgtccaaaa tactaaggga taaagatttt
    10681 gcaaagcttg ctttcgctcg aaaggattct ttctttctgc aacgtacttt gatatgaact
    10741 gcggaagacc ggataaagct aaagtcattg ctatcccata aattggataa acttgctgat
    10801 aaacataaaa accttcgtcg ccgactaagt tttggagggg aattcggtaa agggcactta
    10861 atacttttgc tatgaaagaa gcaatcgtca atacaaatgc tccttgcatg acgcgccgca
    10921 tttctttatg atccaaatca ttgctccttc ctacgctgac agatattttt gctcacgcaa
    10981 cgctttgaca aacttttgga tttcctgaag ccatactgct tcttccattt ggttggggat
    11041 cgtcaagcgt atcaccatct gttctttttc aacgcctaga ctggctttta gttttgtcgc
    11101 tgtcagtgct tcgaataact gttccacact atagcgtttt gttccaactt tacttagagt
    11161 aaagactata tgcgcctcct gtttccttat cgtttctagg agcgctcgat cgccatccat
    11221 tttgatctgc ccaattgtca agagatgtgc tacttcttct ggatattctc caaaacggtc
    11281 gagcagatca tcttctagtt catctagcat ttcttgcgaa tcaagttcac gaattcgttt
    11341 atagatctcg attttctgcc gttgatctgc cacgtatgtt tcaggtagat acgcatcgac
    11401 accgaggtcg atctcaacag tggtcttctc cacttgacta ttctttcctt gtttacgaga
    11461 aacagcttcg cttagcattt gagaatacat atcgaaaccg actgcatcaa taaatccatg
    11521 ctgctgagca cctaagagat tacctgcacc gcgaatggaa agatcccgca tcgcaatctt
    11581 gaatccagat cccagttctg taaaatcttt gattgcctga agacgttttt cactgacttc
    11641 gttaaggatc ttctgctgct catacataaa gtaagcatat gcgacacggc tgcttcttcc
    11701 tacccgccct cgcaattggt ataacgtgga taatcccata taatcagcgt tctcaacgaa
    11761 caacgtattt gcgttcggga tatctactcc tgtttcaatg atggttgtag tgacaagaac
    11821 atcatattga ccttcaataa attcaaaaag cgtattttct aattgcgcct ctgtcatttg
    11881 cccatgagca taaccgatcc gtgcttccgg aacgagttct tggatttctt ctactttttg
    11941 ttcgatcgtt tctacccgat tatataaata gaatacttgt ccacctcgtc ccatttctcg
    12001 atggattgct tcgcgaatag ccccaggatt tttttccata acatacgttt ggatcggata
    12061 acgattcgca ggcggtgttt cgattactga aagatcccgc acgcctaaca tcgacatatg
    12121 caaagttctc ggaatcggtg ttgcagtcag agtcaaaaca tccacttgtg cacgtaactg
    12181 tttcaatcgt tccttatgtt ttacaccaaa acgttgttct tcgtcaatga tcagtagacc
    12241 caaatcgcta aattcaatat cttttgacaa aattcggtgg gttcctacaa caatatcaac
    12301 ttgacctgta cgcagttgtt cgatggtttc tttttgttgc tttcttgttc tgaaacgact
    12361 taatagcccg atatttacgg ggaaaccttc aaatctttcc agcatcgttt catagtgctg
    12421 ctgtgccaag atcgttgttg gaactaaaaa ggctacttgt ttgctttcct ttatcgcttt
    12481 aaaggccgct cgaagcgcta cttctgtttt cccataacca acatcaccta cgagtagtcg
    12541 atccattggt ttttcttttt ccatatcccg tttgatctcc gctgcacttc tcaactggtc
    12601 atcagtttca gagtagggga aggcattttc gaattctttt tggtaggcgt catcaggtcc
    12661 aaaagcatat cctttttctg actctcttgc ggcatataat ttgattagat cgtcagcgat
    12721 atcctcgatt tttgaagaga cttttcgctt ggttttcgtc cattcacttc cgcctagctt
    12781 attgatgcga ggcgctttgg cttcagaggc gacatatttt tggatcagat tcagttgagt
    12841 aacaggaata aacagtttgt cgtcgttttg atacaaaata gtcatatagt cttggtgaac
    12901 gccatctact tctagcgttt ccatcccaat gtatttcccg ataccatgat tggcatggac
    12961 aacgtaatct cctgctttta actcattata gcttttcaat cgctctgcgt ttgttactgt
    13021 ttgacgtctc gtttgttttt tcgtcgtttt ttggaaaatt tctttttctg tgatagctac
    13081 tattttttct tgaggaagtt caaaaccagc ctgcaaagct ccttcaacta attgtacctg
    13141 tcctctgatc aatgcatttt tttctgtttc tactactaat atgtcttcat ctcgaagcat
    13201 ttgttctgct ttgcgtattc tgtcctttgt cggaataaag atcacaacag tctgtttttg
    13261 cttgcgccaa cggtccattt ctgttttcag caatggcatt tgtccgaaga actgttgcat
    13321 cgtccgatat tgaaaattat ggattgcttg gaaacgaaga tttcccatcc ctttttggaa
    13381 gagtgagaaa aaggttgtgg tgaacttttc cttctgcacg attttttgta tatctgctga
    13441 aaatgtctgc tcagaaaaca cccgcaattc gctgattttt tgagtctgcc attctgccgc
    13501 ttccctttcg atttcccgat ttgtttccat gattcgctga taatcatcaa cgactagtaa
    13561 actatttgaa gggaaataat ccaagatcgt cacagattct tggtaaagaa gatctgagta
    13621 gaattttgct tcttctgtcg ggacacctgc ttgccagctt gaagtcagct gaccgaagta
    13681 atcttgcaaa aactcgcggt ctgcttcttc tgtggtcgca gcaagccgtt tttctagcat
    13741 tttttgcatt tgctctattc catgttctag atcctctggt ccgtagactt gttcactcgt
    13801 tggggaaacc cagatttctt ccgttttttc gattgagcgt tgtgtttctg cttcaaaata
    13861 ccgcatagaa tctatctcta catcaaatag ttctacacga acaggatagg ttgtcgttaa
    13921 cgggtaaata tcgataatgc ttcctcgaat actgaattct cccggttttg cgaccatctc
    13981 ttctcgtaca tagcccatca aagtcaattg gcgaggaaga ttttccacgt ctatctcatc
    14041 accaatatgc caattcaatt gcgcctgtag ccaagttttc ttattcggca gtcgtttgtg
    14101 caaagatgcg actggcaaca aatagatacc tgcttttttt tcttgaagtg cgttaagcgt
    14161 tgcaactctc tcgcctcgcg cttctggaga agaaaaggcc atttcagcag aaagcacttc
    14221 atccactgga aataaatgga tctgatcttg cggaatcaca tgttgcagat cttctgctaa
    14281 ctgattcgca taatacaaat ttggcatcag aataatcatg tttttatttg ttttcttaaa
    14341 cgctcgagca aatagaagtg ttttcgctga gcccgctaat cctgtgatta gttgtctagg
    14401 cattttttct tctaaattgc tgacccattc ttttacttgc ggatcttttc cgactaattg
    14461 aataatatcc attccttttc ctccttcctc aattgaatcg attcatcgca tccacgaacg
    14521 aatgcccttc tatgagatat tcgactgctt taacagattg acacatgctt tcttctatca
    14581 aaggctgatt ttctttgctg aaaggactaa gcacatgctg gactactgtt tttttccctt
    14641 ctggcctgcc gattcccacc ttgattcgat caaagacatt cgtattcaaa tgggaaataa
    14701 tacttttgat accgttatgc ccgccggcac ttcccttttg acggagccgg atttttccca
    14761 ctgccagatc caaatcatcg tagattacga ctaattcctc tggataaatc ccaaaataag
    14821 tcattagtgg gccgactgca cgaccagatt cattcatgaa tgtttgtggt ttgactaaaa
    14881 ggatcttttc tccattatga aaaaattcgg caacttctgc ttcgaacgga ttttttttga
    14941 aagtagcttg atgctcgcgt gcaattttat ccacgaccat aaagccgata ttgtgttttg
    15001 tatattggta tttggtacct gggtttccta acccgacgat cattttcatt tttgttttcc
    15061 acctaattct aaaaagcagc acaaaaggct ggactgctaa ttagcggtcc agtccatcga
    15121 gcttttttct tttttacact tgtcaatgca tagtataaca caaaacaagg cgtttcggct
    15181 aaaagagaat cgttaataaa tgataaagaa attaaaaatt tcttcaaagt ttaatgaaat
    15241 aattgatata taaaaatagt gaaataacgc acatgtaagc ttttacatat aatacaacct
    15301 tgttatctgt tatattttat agccaaatag cgtgacatgg ggcttgagaa atgttagcat
    15361 aagcatggga aaaaacaaat caatggaagg attgatacta aatgactgca gctgcagaaa
    15421 aaaaagatca tcaaaaagtt attcttgttg gagacggcgc cgtaggttct agctatgcct
    15481 ttgctctagt tactcaaaat atcgctcaag aagtaggtat tgtggatatc aatactgcta
    15541 aaactgaagg agacgcaatt gacttgtctc acgctttggc atttacttca cctaaaaaaa
    15601 tctattctgc tacttacgca gacgcacatg atgctgactt agttgttatt acagctggcg
    15661 caccacaaaa accaggtgaa actcgtttag acttagtaaa caaaaaccta aaaatcaacc
    15721 gcgatgttgt cacacaaatc gttgaatcag gatttaacgg tatcttttta gttgccgcta
    15781 acccagtaga tattttgact tactcaactt ggaaattctc aggtttccct aaagaacgcg
    15841 taatcggttc aggtacctct cttgactcag ctcgtttccg tcaagcactt gcggaattag
    15901 ttgacgttga tgcacgtaac gtccatgctt acatcttagg tgaacacgga gattcagaat
    15961 tcccagtttg gtcacatgca aatgttgccg gcttgcaaat ctatgaatgg gtaaaaaaca
    16021 acccagaaat cgatgaagaa gctatggtta acctattctt tggcgtacgt gacgctgctt
    16081 acacaattat cgagaaaaaa ggcgctactt tctatggtat cgcagtagca ttagcacgta
    16141 tcactcgtgc catcttagat gatgaaaatg ctgttcttcc tttatcagta ttcatgaatg
    16201 gcgaatatgg attgaacgac atctacattg gtgcacctgc tgttatcaac cgtcaaggta
    16261 tccaaaaagt aatcgaaatt cctttaagtg acagcgagca agaccgtatg gctgcttctg
    16321 ctaagcaatt aaaagaaatt ctagatgaag cttttgctaa actagacgct gaataatctt
    16381 tgaacatgac ggaaaacatc tattcggtgt tttccgtttt tttattttat cacccttatg
    16441 gtttctaaat tttttcataa agtagagata accttcgtct tttcctctaa attatgctat
    16501 catgagaccg ttccacccta aacttttact taaggagttg tcgatatgac aagatcttcg
    16561 catcgaaaaa aatcgaaaca tgttgctttg ctttcagttc ttggtgttct cttagtgctg
    16621 atcagctttt atacctatcg cagtacatac tacacaaagc attttttgcc gaatacagaa
    16681 ataaatggaa taaatgtcag caatctaaca gtcgaaaaag caaatgaaaa attaaaagaa
    16741 gcttattctg ataaaaaact ggctattaaa gaagatggaa aagtctggca ggaagtacca
    16801 aaatccgagc tcggctacaa agatgatttc acttcggagc tttctccgat cctaaatgaa
    16861 caaaacggat ggacttgggg aatgacttat gtctcagcgg cagaaaaaca agaaatagat
    16921 cctgtcagtc aaaaccacca aaaactagac actaccgttc agacattgac aactaagctg
    16981 actgatttga acaaagaccg gacacctact actgatgcaa cgatcgaaaa agcaggtgac
    17041 tctttcgtta tcaagcctga agtcaatgga aatacgatcg acgtcgatgc tgcggtcaaa
    17101 caattaaaaa gtgcggtaaa ctctggtaaa gatacaattg aattgactga attcaaagaa
    17161 aaaccaaaag taacatctga agacagctca ctgaaagaac aactcgcatc catgaatgct
    17221 atcgcaaatg taaaagctac ttacagtata aacggtgaga ctttccagat cccatcaagt
    17281 gatattatga gttggctgac ctacaatgat ggaaaagtcg atttagatac agaacaagta
    17341 cgtcaatatg taacagattt aggcacaaag tataacacaa gtacgaatga cacaaaattt
    17401 aaaagtacaa aaagaggaga agttacggtc ccagttggca cttacagttg gacgatccaa
    17461 acagacagtg agacagaagc attgaaaaaa gcgattctgg caggccaaga ttttactcgt
    17521 tcacccattg tgcaaggtgg cacaaccgct gatcatccat tgatcgaaga tacgtatatc
    17581 gaagttgatc tggagaacca gcatatgtgg tattacaaag acggaaaagt ggcccttgaa
    17641 acagatatcg tttccggaaa gccaactaca cctacacctg ctggagtctt ctatgtttgg
    17701 aataaagaag aagatgcaac tttaaaagga acaaatgacg atggaacacc ttacgagagt
    17761 ccggtaaact actggatgcc gattgactgg acaggtgttg gtatccacga ttcagactgg
    17821 caacctgaat acggtggaga cttatggaaa acccgcggtt cacacggctg catcaacacc
    17881 ccaccaagtg taatgaagga attattcggg atggtcgaaa aaggaacgcc tgtattggtc
    17941 ttctaacaaa aataatatat aaaaagaaag cacccgtacg attggtacag gtgctttctt
    18001 tttattatct aacaatgtgc tacttacatt tcagcagcag taaagacttc tgaaacatca
    18061 tcatcgtctt ctagcttatc tactagacgt tccaattgct ctttttgttc ctcatttaaa
    18121 tcaatcgtcg tttgtggaat catcgtcaat tctgcttgcg ccaaagtgaa ctctttttcc
    18181 aactcatctc gtacagcagc aaaatcttct ggtgcagtat aaatctcgaa tacttcagga
    18241 gaagtttgca gatcttctgc ccccgcctct aatacatcct caaacatcgt ctcttcatca
    18301 acagtcaacc catcgcgttc aatagcgata tatcctttgc ggtcaaacaa gtaactgact
    18361 gaacccgttt ctcccagtga tccaccgttt cgagtaaaag ctactcggac atttgtcgct
    18421 gtccgattac ggttatctgt caatgcatgg actaagattc ctactccacc cggaccatat
    18481 ccttcatacg tgatttcatc gtaattcgtt tcatctacgg cactcgtagc ctttttaatc
    18541 gcccgttcca cattgtcgtt aggcatattt gcagcttttg ctttgtccac aacaagccgc
    18601 aaagctggat tcatagatgg atcaggaccg cctgctttag ctgccatata aatttcacga
    18661 gaaagtttct gaaagacttt ccctcgtttt gcatcttggg cattttttct tccttggata
    18721 ttatgccatt tactatgtcc tgacatgatt tttcctccct tgttcttttg agaatacact
    18781 tctattctag tactatttca caaaaaaaca aagccacagc caatctataa acgcttcttg
    18841 aatgccgcaa aaagcacaca taatatcaca gcagtcagag caccgatcga atccaacatg
    18901 acatcttgga acataggtgt tcgtccaccg gtcatcatct gatggaattc gtccaaacct
    18961 gcgtaccctg ttgctgaaag ccaagcaagg atcgctgtca gccaccaaat ttttagcttg
    19021 gggaaaacac ctaaaaacaa gctgcctccc aaaataaaat aggtaccgaa atgtgcgccc
    19081 ttccttaaaa agaactctaa aaatttggcg tagcccagat ggttgatact caccatactt
    19141 ccaccataat taaaacgtat acccgctaat gtgtctttaa atggctgatt aggcagccag
    19201 ctttctaatc gcgaaacttg tgattgctgt ccgtaagttt gagaagaact gtaaaataag
    19261 attataacga ctatcagtgc gattactaaa taaatatttc cgtttttcat ttgttttttt
    19321 gaagaattca taagtcacct ctgtatttta tcttagactt tttttgaaga aacaacaatc
    19381 attttcatag agacttcttc cataaaaaag tgctatactt aattttgtat tacaatatga
    19441 agggagtttt ttcatgagtt attcattaaa ttggaatcta gattctattt tttccggagg
    19501 cagtcattct gatgctttga accaacggat gaagcaattg gaagaccaaa tgaacgagta
    19561 ttatcaccgc gtaacaaaat ggtcacctag ttctgacaat gcagagcagt taaacgctat
    19621 tcttcaattg caagaaacga ttacgaatgg ttttacccaa tgcagcagct atattactgc
    19681 attgctttct gccaatgtca atgattcaga tgcaaaggtc ttatcaggca agctttacgc
    19741 aatgctgcct cgtctgcaat cagccgaaac tgtattgtca aagaaatttg cagaaatatc
    19801 tgacaatgat tggaatcaga tgctttctca atcttcgttt gaaacaattg cttttcgttt
    19861 aaacgagatt cgtcgtgatg gctcacaatt attatcagaa gctgaagaaa acattataaa
    19921 tacgctttct ttagatggtc taaatgcttg gagcagtcat tatgatacga ttgtagccag
    19981 catttcgatt cccttcgaac aagatggaca agtagtcgaa ttatctgctg gacaagcctt
    20041 caataagatg atgggcgatc cagatccaaa agtcagagaa acacttttcg cagcatggga
    20101 agaagcctgg aaagaaaagg cacccctctt cacggatact ttgaatcatt tagatggttt
    20161 tagactttct gactatgaac ttcatggtgt cacagatttt ctacaaaaac cattagagta
    20221 caatcggttg aagaaagaaa cattgtctgt catgtgggat acaatccaga aaaacaaaca
    20281 accccttgtt aattacttga ctagaaaagc taatcttttc ggtaaagaaa aaatggaatg
    20341 gcaagatcaa gatgcaccta ttattttagg cgatttgaaa gaaaaaacct ttagttttga
    20401 cgaagcagca gcatttatta tcgaaaactt taacaaattc agtccaaaaa tggctaaatt
    20461 tgcccaatct gcttttgaaa agagctggat cgaagcagaa gatcgtcctg ggaaacgtcc
    20521 tggtggttat tgtactgagt taccagaaac aaaagaatca aggatcttta tgacctacag
    20581 taattctgtt aatgaggttg cgacacttgc tcatgaacta ggacatgctt tccatagcag
    20641 cacgatgtgg gatttacctt ctttaaatcg cgaatatgcc atgaatgtcg cagaaacagc
    20701 aagtacattc gcagaactga tcgtagccga tgctacatta aaagtcgcaa aaacaaaaga
    20761 agaaaaaatc aatttgctag atacaaaact gcaaaatgct ttagctatgt tcatgaatat
    20821 ccattcccga ttcatttttg aaaatcgatt ctatgaagca cggcaagaag gattagtctc
    20881 agaagataag atcacagaga tgatggttga ggcgcaaaaa gaaggctatc aaggagcttt
    20941 agctacttac catccatatt tctgggctgc aaaattgcat ttctttattg atgacgtacc
    21001 gttttataac ttcccataca catttggcta cctctttagt ttgggcatct atgcttacgc
    21061 caaccaaaag ggagcaaatt ttgaagaaga atatatccat ttactacgcg atactgcctc
    21121 aatgacgacg gaagatcttg cgaagaaaca tttgaatgtc gatcttaccc aaccagattt
    21181 ttggcaagca ggtatcgaca tggtgactga agatatccaa acgttcatgg aattgacaga
    21241 agaatttatc tgatttctat tttatcacaa taaaaaaaga gttagtccag aatgggctaa
    21301 ctctttttga ttaatcattc ttacatgctg acagcaaagt caggggcaaa ccattgaact
    21361 gaaccaactt ttacgctttc gcctggttga ggagcatgga tatattgtcc tccgcctaaa
    21421 gcaatcgcta cgtggtaagt tccgccttgt gacccccaga ataacaagtc tccagctttt
    21481 gcttgagaaa ctgaaatttt tgttccagct gattcttgag gaactgtcca accaccgatg
    21541 tcacgaccag ttacttgcat gtaaacatag cgtgtgaacc ctgagcagtc aaagccactt
    21601 ggatctttac cgccccaaac atatggagta ccaatatatt tgtaagcttc tgctacgatt
    21661 gctgcaccat tcacacttcc agaaggagct ggagcaggtg ttacaggttt tggttgttca
    21721 ggtgttggtg ttggcgttgg agccggagta ctacttcctg tgccgtttcc tgtatcaaca
    21781 ctttgatctg ttgatggtgt tgtaggcgca ggtgttgttg attcttctgt tgttgaagat
    21841 tcaggcactg ttgttgattc ttctgttgct gaagattcag gcactgttgt tgattcttct
    21901 gttgctgaag attcaggcgc tgttgttgat tcttctgttg ctgaagattc aggcgctgtt
    21961 gattcttctg ttgaagattc aggtgttgtt gattcttctg ttgctgaaga ttgtgtagct
    22021 gatgatgatt cttcagtcat tgaagattgt gtagctgatg attcttctgt tgctgagctt
    22081 tgtgcagtag atgaagcttg agtagcttgt gcttcagcct ctgcttgttc gcgtgcttct
    22141 ttttcagctt tttcttgtgc agcttgttga cgagcttgtt ctgctagacg ttgttgttca
    22201 cgaatacgag cttgctctgc ttcagcttct gctttttgac ggttcaagtc agctttcttg
    22261 tcttcagcag ttgcttgttc agcagctaat gaagttttca gtacgttcaa atcagcttgt
    22321 ttagataaaa gatcgccttt ttgagattcc aaagcagctt ggttttcagc taattctttt
    22381 agtttagcat cgttttcagc ttttttgtct tctacagctt ttttatcttg tttttgttgt
    22441 tccatcaagt cgttgtttgc ttttaccatc gttgtcattg cttgaacgcg tccaatagca
    22501 tctgctaatg aatcagcatt caatacagca tcgatgtagt ttgagcttgt gttgcttact
    22561 tgtgcttcgc gtgcttgttt ttggattgta tcttcacgtt tttcaatacg ttcttgtaaa
    22621 tctgcaatgt cttttactaa ttgtgcagat tcttgacgca atgtatcttg ttttgctaat
    22681 aggtcttgtg cttgtgtgtt gatttcagaa acttggcttt ctaaggcatc gatttgtgat
    22741 tgtgcatctg cttgttgatt tttcaagtct gcaatctttt gatcttgctg ttgtatctga
    22801 gaatcaaaat cgtccgccgc ggcagcgatc ggtgatgcta cggcagtcaa ggtcattgaa
    22861 cataccatta ctgctgatat taaactcttt ttcactcttc attcctccga ctggcttaat
    22921 ttaataaata tctaaatata tttaagtctt tcttaacgat ataaaaattg taccatagca
    22981 gtgtgtcaac gatataatag ttatgttaca aaaatgtttc aaaacaactt ttaaaaatga
    23041 caaaactgtt cgtttcagaa aatgatcatg aaaaagccgc gttaagtcta gcatttctta
    23101 agataacaac gattcttttt cgctttcgac ctaaaaataa aaaaagaaat tattcatcac
    23161 tgaataattt cttaaaggga aaaatgaata atacaaatat aatcatgttc aacagcagtg
    23221 taggtcctaa aaaccttgta ataaaatacg tgttatttac tactgctaat ttaaaaatca
    23281 actggaccac catcgtaaac aattcataac ccgtgacaaa gatgatcata ctgaaaaatt
    23341 cagtaaagat gttgacatgg ataacgtctt tcattacata catcaaccat acaattaaag
    23401 ggagagctac tgcatagata ccgataacgc caatatagta agcgtcatag atagccccga
    23461 gaacaatcgt ggtaatcagt aaataacgtt tttcaaacgc catactgcaa cataataatg
    23521 ccaatatcag aaagtgggca tttgacatat aggtgccttt cgaccactcc cctagcattc
    23581 ttgttaaatg accatctatc aacatcaaaa agaacaaaac gattggcaga tagtacttca
    23641 ttgtttcttt cttcaacatg gactaatcct ccgctagccg ttggacaatc gtcacaactg
    23701 acacgtcata catttctgca taaggtttca cataaacttc ccgatccagc ccataactat
    23761 ctggtttcgt tttaataacc gtaccgatcg gcaagttagc tggagaattc cctccaagac
    23821 cggatgtctg gacaacatcc ccttctttga tatccgtatc accagttaat tgggtcacca
    23881 ctaaggcatg gagcttttca tcatagtttt taagcaaacc aaacgcttcg ccattagccg
    23941 aagatacccg tactggaaaa tgattggagc tttcattaga ggatgacagt aattcgattt
    24001 tagacgaagc cgtattgacc tcgatcactc ggccaatcaa tcctttttgc gacatgactg
    24061 ccatatttgc ttcgataccg tctttagaac ccttgtctac gatcagcatg tcctgccaac
    24121 tgtcaggcga acgagtaatg acatttgccg ttactttttc gtaactagtc agcgtctgat
    24181 tcaactcaag ttcttttttc aacgcttcga tttctctctc gtaattcttg ttttgctgtg
    24241 aaacgtcatc atagctatcg attttttctt ttagccgctc attttcggca taagttgaaa
    24301 aaagattatg gacagaatct actccatttc gtacccaccg tactggggca gagattgttt
    24361 tatcaaccat tgcaacactg tcattaacga tggattggaa gaaattgggc tttccttcat
    24421 tcgctcgctg cgcagcggtc acacttagta tcgtcaccac gatgataaca ataaccaagg
    24481 taataataat atttttatta ggattgaact tcttcacaaa gacacctcga attaattaga
    24541 ctcttgtgtt caattttatc actaaatgcc gtgaaacaaa aggtaagttg ctcctcttaa
    24601 gctttttttt agcaaataat agattctgtc atttttagcc tgaaagacaa atttttttat
    24661 ttgtttatgg atcaatcttt gctttgaata actgcaatca taccatctga aaaagaaaat
    24721 tctgcggtat ctgaaagaaa ttcgttactt gcataagatg tcggatattc tacttcttta
    24781 ttttccaata catacttgct gccttttaat gtaagattcg agacgggagt caagcagcaa
    24841 taagctaaat acttcatatc tggtatctgt cttactatat gctctccagg caaataataa
    24901 gacaaataat tttgacaatc tctgataata agctgatggg caaagggttt gaagcgaggt
    24961 tccataccta accagagatt ggctaaaaga tgatccaatc ttccgcccgt agctccaatc
    25021 aaagtgactt ccgcttgcgg gtaacgttca aatgttttta agatacctaa ctgtgtatct
    25081 gtatcgtctt tttctgcagg ggaacgggta tactctttgg cttttgcaaa tacttccttc
    25141 tgctcttcgt tcgacaatga atcaaaatca cccactgcca gatctactgg gtagcctgct
    25201 tgaagcaggt acagcgcacc acggtctatc ccgacatagg catcgtattt ggacagcaga
    25261 gactgcggcc agttttgcgg attacctcca gcagctaata aaattttcat ttagtctaat
    25321 gcctctttta atcgttcgat ttgtccgact ggattttcgg ctccatagat atatgatcct
    25381 gcaacgaaga catccgcccc tgcatcttta cagatttttg ctgtttctgg aacgattccg
    25441 ccatctactt cgattgcata agtccaattg ttctctctac gtagatcagc gagttccgta
    25501 attttttcta ctgtttcttc aataaagctt tgtccgccga acccaggatt gacagtcatg
    25561 actagtactt gatcagccaa aggcaaaaca tgtttgatca tggagactgg tgttcctgga
    25621 ttgataacta ctccagcttt aactcctaag ctttttgtca tttgaagtgc ccgatgaata
    25681 tgaggagttg cttcgacgtg gacagtaatg atatctgctc cagcttttgc aaatgctgga
    25741 atgtagtttt ctggatcaac gatcatcaga tggacgtcta acggtaattt agtaacagga
    25801 cggattgcct gaacaatatt tggccctaat gtgatatttg ggacaaatcg gccatccatt
    25861 acatctacgt ggatgtaatc tgcgcctcct ttttccacta actcaatgtc tctttgtaaa
    25921 ttggcaaaat ctgcactcaa aatggatggt gctatcttca ttcttgtttc ctcctatttt
    25981 cgtttacctt ctttttttgc atacattggt tttctttttt cgatttcttg taagaattgc
    26041 aaataattgt cgtaacgtgt ctgtgcgata ccacctgttt ctactctatg ttttacttcg
    26101 cagccaggtt ctttagcatg catgcactct ctaaatttgc aatggacaga agcttctaca
    26161 aattcaggaa attgtctcgg cagttctcct gcttcaattg tcaaaaagtc aatcgagcta
    26221 aatcctggtg tatctgctac taacccgcca tagataggaa tcagttccac atgacgtgtt
    26281 gtatgccgtc ctcttccaag tgcatcagaa atctctcctg ttgctaaatt cagttccggt
    26341 gatatttgat tcagtagcgt cgattttcct gctccagact gacccataaa gaccgatagc
    26401 cgttctggga agtaacggat cagttctgtc gtatcacccg tatgattggg tacaatcacc
    26461 ggatagccta ttttagtata ggtttgcttg atctcttcga ttttttctgg gtcgtctgac
    26521 aagtcggtct ttgtcaaaaa aataatcggt tcgatcgact cgtattccaa ggtaactaga
    26581 aagcggtcaa gtagattata agaaaatgca ggctctacga tacttgtgac aatgattcca
    26641 agatcaacat ttgtgactgg tgggcgaact aattgatttt ttctcggcaa gatttccaga
    26701 agatagccgt ctgtttgatt ttcactctca aagatcacct gatctccgac aagcggcgtg
    26761 attttccgat tccggaaatt tcctcttcct cttgtttgat aggtctctcc gtttgcgtat
    26821 acatagtaaa aaccgctgat tgcttttctg atttgtcctt tgataagtgc cacctccgtt
    26881 tttgtttatt ttaccatacg aagaggaaag taggtgttat tccatgcaaa cttctcaaag
    26941 tatatggctt gaaaattgat caaaagagat agaaacacaa aaaagagaat gcaacaaatc
    27001 aatgtcgcat tcccttctga tatattttat tcagcactta ttttgaactt tctgaggtgc
    27061 tagctgatga acttgtggag ctggaagatt cttcttttga agagttgctt gtgctactgg
    27121 agctacttga gctgctagaa ctgtctgaag agtctttttc agaactagaa gacgctggat
    27181 ctggtccttt agagtaaatt acgttcacaa ccattccttt ggtaattgtc gttcctgccc
    27241 ctggtactgt acgaatcact ttatctttat ccaccgtgtt tgaatattcg gaagtctcat
    27301 gcccgatata ttctgcaccg acactagcta aataactttg tgcttcgctt tttgtatagc
    27361 cggagatatc attcaattta ggctctgttc cttggcttac gacaaaattg atgtaagttt
    27421 cttctgctac aacttcaccc gaggctggat cctgactgat gatcgaacct gatggtacag
    27481 tgtcgctata gtcataagta gcactaatat tattgatatt gaatccttgt gtaacaagcc
    27541 aattacgaac agtttcgtag ttttctccta catgactact caaagtcact gtttctggtc
    27601 ctttgctgac tgtgaaagta atcgtgtctg ttttaggatc gacttctgta tcttctgatg
    27661 gagattgcga aatgatcatt ccgctttcta cctctgaact gaattcctct tctttttgaa
    27721 tcaagtcagc agaaaaccct aattctttca aacttgcttc ggcatcttca taacttttac
    27781 cactgtaatc atctaatttg atttttttcg tgccgctact aatatacaga gtgacttcgc
    27841 gtttttgctt gactgtcgtt cccgctgccg ggtctgtctt cactacatta ccttcttcga
    27901 ttttgtcatc tgcaatttct tctgtttttg ttgctacttc taatttcgct tttgcgagtg
    27961 tttctctagc agaagcttca gaaagatcag aaacatcagg aacaacaact tctcctcgcc
    28021 cgccagaagc aaagaataca gctgttccga ttcctaaaac agcaagaata gctaatagga
    28081 tcagccacca ttttttctgt cctttctttt taggatcagg cagaacttct tcctcctctg
    28141 gtaaggtttc gttttctggc ttgtcttttg gcagaggcat agatttgaat gaatcaggca
    28201 ttggtgcatc ttccgtaagg ggtgtgataa cttttgtttc attatccatc acatgaggct
    28261 gccatttttc ttcgtttgca cgagctggtg ataatactgt agaaagatca tcagacattt
    28321 cctcggctga cttataacgg tctgcaggtt ctttagccgt tgcccgtaaa atcacgtttt
    28381 ccagtgcttg tggcacattt ggatcaagtg cttttataga tggaagatca tcttgaaaat
    28441 gttttaaagc aattgttaca gcagattcac catcaaacgg tacgctaccc gtcaacatct
    28501 cataaaggat gatccctaaa gcataaatat ctgactgctt cgttgccata cttcctctgg
    28561 cctgctcagg ggacagataa tgaacagatc ctagcatcgt attcgtctgc gtgatcgatg
    28621 tttcagataa agcgatagca atcccgaaat ccgtgatttt tacaacacct tcattatcga
    28681 tcagtacgtt ttggggtttc aaatctcggt ggatgatttg atggttatga gctaaagaaa
    28741 tagcagaaag gatctgctgc ataatattta ctgctgtttc ataaggaaca ggataatgtg
    28801 tttggatata tcgtttcaag tccattcctt tgacgtattc catcaccaga tactgcatgt
    28861 tgtcttcttc accaacatcg taaacactaa caatgtttgg atgaacgagt tctgttgctg
    28921 ctaaagcttc tctctgaaat cgtcgaatcg cagtctggtc attttgaaag tcaaatcgta
    28981 agactttaac tgctacatca cgatccaaga ttaaatcgtg cgccaaaaat acatttgcca
    29041 taccaccgct accgatattt ccagtgattt gataacggcc atttaatttt ttgcccaact
    29101 cgatcataca ctgtcctcct tatattcgat aagaagaacg gtgatattat ccgctccgcc
    29161 tgcttcattg gcacgatcaa tcagctcagt tactttatga gacaatgggc cttcttgatt
    29221 gatgatcgtt cctatttctt cttcagacag catgttcgtc aagccatctg aacataacag
    29281 cagtcgatct tttagttgcc aaagatgagt agaaacatct acttcgactg ttccaggcat
    29341 gccgacagaa cgtgttaaaa tatttttcct tggatggttc actgccattt cttcgctgat
    29401 ttctcctgat ttgactagtt cattgactaa agaatgatcc tccgtcaatt gcatgatttt
    29461 atcttcacga atcaaataag ctctgctatc cccaacattg gcaatcgtga accagtcatc
    29521 tgacagagcg gccgctacga ttgttgttcc cataccgtta tattccggct gttcttgtcc
    29581 acgctgataa atcagcatat tttcttcttg aatcgattga atgaaccatt gggcgatttt
    29641 ttcatcgtca acagcttctt gtttttccca agcttcgcct agattcgtta ccgccatctg
    29701 actggcaatg tctccggcac gatgaccacc catcccgtct gctaatacag caagcttgag
    29761 acctgaacgg ctggtaaata cactggcata gtcttgattt gtgttacgtc gtcttcctac
    29821 atctgattga tattcaatct gcattttttc acctcattac tttttgcgca aacaacaaat
    29881 gaaaaatcca tctgtgtaaa actgatgcgg atacaaagtc aacattttct cttcgattga
    29941 tgattgtagc ataggattgg tcaaaacatc aatcctctca aaatcagggt gtctttccaa
    30001 gaaagcagaa acaacgtctt ggttttcttc ttttaagatc gtacaagtgc tataagtcag
    30061 aataccggaa gactttaaag ttggtgcgca gctttctaaa atttccaatt ggatttttgg
    30121 taaattcgct aattcatccg gctgtttttt ataacgaata tctggttttc gccgaagcag
    30181 cccaagacct gaacaaggcg cgtctactaa aatccgatca aactgctctg cttcgaactg
    30241 tttactgact tctctagcat ccatcttttg agcgtagaca gcagaagaga cacctagacg
    30301 tgctgcatta tcttcaatga gtttgacttt gtgttcgtgt acatctaaag cgaccacttc
    30361 tccaccgcat gacggatcta aaaaagaagc aatatgcgtc gtcttacctc cgggtgctgc
    30421 acaggcatct aagacatgat gttcaggctc gatttgcata gacggcgcta caagcatcga
    30481 actttcatct tggatcgtca tgacgccatc gtggaacaat gagctagaag ctaaatgccc
    30541 ttttttcgct actataccgt atggactgac ttggcttttt tcagctgtga tctcttcttc
    30601 ttgcaaaaca gtgatggcct gatctctgga aagtcggcgt gtatctactc gggcactagc
    30661 atggctaggc tgatatagcg ataaaccgag cttttcagtt tcttcgtatc cgatttgatc
    30721 caccagtcgt ttcgtcatcc atagaggcag gctgattttg acagaaagtc tttcgatagg
    30781 atcagcaatg ccttgcagat ctggtgctcc ttgacgctga taattcctca agacaccatt
    30841 tacaaatttc cctgtcccag gatttccttt tacttttgcg atttcgaccg cctcgtttaa
    30901 aatagcatgt gcagggactt tatctaaaaa ttctaattgg tatacagaaa gataaagaag
    30961 catcttgacc cagtcatcca ctttttttgc tttagcgatc aatggtgcaa cgtaaaattc
    31021 caatagtcgc tgatggctaa tcgtaccata gatcaattct gtaaataaac ccacatcttt
    31081 ttgatttagc tcgctttgtt tgatcatctc attcaacaaa agattcgagt aggcgccacc
    31141 cttgttcact cgctctaatg tcagtaatgc catatatcgt actgattttt tcattttttt
    31201 agggattttt ttcattattt cacctgctga ccaatctgaa cttgctgacc gcttccgtta
    31261 aggaaatcat gtacggcctg ttttccttta ccagctggct gtaattcatt gatggctaag
    31321 acggtctgtt taccgcaagc aatccataga ttttttttgt ctttccggat gatcgtacca
    31381 ggtgctttgt ctgtagtttc tgccaatact tcaaccgata ataatttcca gcgtatgccc
    31441 tcataggtcg tgaatgcaat tggccaagga cgcattccgc gtacttggtt gtcaatttct
    31501 tctgcagttt tatgccaatc gattgcttct tgttcccgtg tgatgtttgg cgaaaaagtt
    31561 gcttctgact cgtcttgagg tactggtttt tgtccatcca aaatattggg caatgtttct
    31621 agcaatagct ttcttcctaa aaggctaagc ttttcaaaca tcgtccctac atcatcttgt
    31681 ttagtgattg gtatgctttc ttgcgcatag attccgcctg catccatttt tttgatcatt
    31741 tccatgatcg ttacaccagt ttctttctcc ccattcatga tggcataatg aacaggcgca
    31801 cctccccgat atttaggaag taaagaagca tggacattga ttgcccctaa ttttggtact
    31861 tgcaataact tttcaggcaa gaattggcca aatgccgcag tgacgatcac atcaggcacc
    31921 aattcaatga tttcttccat ttctggcgaa ccggagattt tctctggctg cagcacacgg
    31981 ataccgtgct tcagagccgc tttttttacc ggtgtcggcg taattactcg tttccgccca
    32041 actggacggt cgggctgtgt taccactgcc tggatctcgt aacctgattc tatcagccct
    32101 tcaagaatcg gcacagaaaa agcaggtgtg cccataaata ctagcttagt catcaagatg
    32161 ttcctccata tattcttcaa gttcttcttc cgggatctgc tgtatcattt tctcaatgaa
    32221 caagataccg tctaagtgat cgatttcatg ctggaaggca cgggctaaat agccaaaagc
    32281 cgtcacttct atctcttctc cgtctcgatc ataataacgc acggtcactt catctgctcg
    32341 cttgaccgtt ccatagacat gcggaatact gagacaacct tctacgtcaa ggctttcacc
    32401 ttttgcttcg atgatttctg ggttaatcag ttcaaatttc tcgccttcat ctacttcgat
    32461 cacagcaatt ctcttgtttt gtcctacttg aggagcagcg atccctattc cgtcattcgc
    32521 aatcattgtc tcatataaat tgtctaataa agcgattgtc tcgtccgtga ttacatcgat
    32581 tggctgagca gttcttttta atttatcatt agggtggatc aatataggat agcgcataat
    32641 ttggcccttt cttaaataaa gtttaatggt tcgttgtcaa ttgccacaaa aagaccttgt
    32701 cgctgttctt tctggcttcc attcaaaatt tcatccaaaa catgaggaag attcggttca
    32761 tgtttatatt tgatgattag ttgataataa tatcggtttt tcactctcaa aatcatgctg
    32821 ggcgttggcc ctaatagcaa gctttgaggg cttaacactt cttttagctg attggcaatc
    32881 tgaaagattt tctttgccgc tacttgttct tcaggatggc tggcggttat tttcacagta
    32941 aagtaataag gaggatagcc tccacgatgc cgtacatgca tttcctgctg ataaaactgc
    33001 tcataatttt gttttttagc caattctatg gcgtaatgtt gaggattgaa tgtttggatc
    33061 acgacttcgc ctgctttttc cgctcgccct gctcgtcctg aaacttgggt aagcaattgg
    33121 aatgtatgct cactagaacg aaaatccggt aaattcaggg aggtatcagc attcaacacc
    33181 ccaactaatg tcacttctgg aaagtccaaa cctttagcaa tcatttgtgt ccctaataat
    33241 atatctgctt cttttgcacc gaatttttgt aaaatctgtt cgtgtgcccc ttttcttcga
    33301 gtggtatcta catccatacg caaaatccta gcttgcggat aaagttctcg gagttcctct
    33361 tctacttttt gagtacctgt tccatagtag cggattttat tgccgccaca attaggacag
    33421 cgattgggaa taggttcttc atgtccgcaa taatggcagc gcattgaacg tgtatccata
    33481 tgcagtgtaa gagagatatc gcaattagga catggaagaa caaatccaca gtccctacac
    33541 atcacaaaag acgaatatcc tcgacgattg agcagtagga cagtttgttc ttttttgtct
    33601 aatcgatttt gtattttttc ctgcaaatta gcagaaaaag tagaggtccg atgattctga
    33661 aactcttcac gcatatctac gatctcaata gctggaagct gtgcagctgc tttggctcgg
    33721 tggtttaact gcaataactg ataaacgcct ttttgagctc gtgctcttga ttctaaagag
    33781 ggcgtcgcgc ttcctagaac tattgggcaa tgatgatatt tactgcgcca aattgctaga
    33841 tctctcgcat gataacgcgg tgtctcatct tgtttgtaac ttgattcatg ttcttcatcg
    33901 ataatgatca gcccgatatt tttcagcggg gcaaaaatag cagatcgtgc gcctactact
    33961 acttgcgctt cttcccgctc aatcttgcgc cattcatcat atttttcacc ttgggaaagt
    34021 ccgctatgta agaccgcaac tgattctccg aagcgtcctt tgaatcgctc gaccatttgc
    34081 ggagtcaatg cgatttcagg cactaacata atagccgttt tattttctga caaaacatca
    34141 gcgattgcct gaagatatac ttctgttttc ccgctgccag tgattccctc cagcaaaaag
    34201 acctgatctt tttgctgctg acctgctgtg atgatttgtt cgacagcatt tttttgttct
    34261 gcattcagtt ccaatgccgt cgtttggtca aatactcggt ctttataagg gtcgcgataa
    34321 cgttctgatt ctataaactc cagccagccc ttttcttttc cttgattcaa gacagcagta
    34381 ctgattcctt tatttttgaa ataagcaatt ggttcttctt ggctcagttc agataaacac
    34441 aaaagaagtt ctttttgttt cttggcattt tgccgtaatt ttgcccattc ttcttcgatt
    34501 tgcttttcag ttaacaaaga acggaccaaa cgaatcgttt ttactttatt tcgtgtatgg
    34561 acttcatatt tgatatctac taattgctct tggcgccagc gaatcaattg agaaaggctg
    34621 ccttcttctt ctgcttcctt ccaggaaatc acttctcgct gatcaaacaa tacctgaact
    34681 tctggtgtgt ctgccaaagg atagatcaat ttatcatatt ctgctttcat cacactcgga
    34741 agcatcgttt gtagacaagt gattttgaat gcgtaagtaa tttttttcat ataatcagct
    34801 aatgaaagta attcttcatt gaccaccgga gcaaggtcta gcaaacgaat aactgccttc
    34861 aaagatggat tagctgattc ttctgatttt tggatagcca taacaaatcc ttggacatga
    34921 cgattgccat taccaaaagg gacttctact cgcatcccga cttgcaaagc tgtttccatt
    34981 tcagagggaa ccaaataagt gaatggctga tcggtctgca tagtaggtac gtccacaatg
    35041 acttcagctg ttaatgtcat ggttcgtttc ctcctttttt tgtcttttct tatcaagcga
    35101 caaaaagttc cttgcctatt ttactagaga tcgatggatt tgtcttatct tctgccaaat
    35161 cctaacaaaa aattaacagc agataagaaa aaaaagaact gtgtcaaaaa gttcagacac
    35221 agccttttct accagacaat aagtggcgaa tgtttgaaaa ggaaacagct gggtttgctt
    35281 ttctttttag atgttcgctc tatttttctt tattacattt tttgctcttc acggatacgt
    35341 gattccaatt cgtgttgttc acgctctttt tgtgcttgac gttcctcttc ttccatcttc
    35401 aaacgttcac gttttaattc aggatgtgga tcattgacta catttcctgc atcgatttct
    35461 tctaaagctt gaccgacgct ttttactgaa tcaaaagaat ctagagtagg ctgtgcactt
    35521 gcatccaatt catgcgcgcg tttacttgct aaaatcacta gagaatattt tgaatttaca
    35581 cgatctaata gtgagtcaat tgaaggtttt aacatcataa gactacatct cctttaacat
    35641 tttaatatat ttaccgatta cgcgatctac tcgaaaatgt tcactggcaa taatgttttt
    35701 gattcgttca actgctagcg gcacttcatc atttacaaca gcatagtcgt aaagagccat
    35761 catttcgatt tcttcttttg caactttcat tcgtttttcg attacttcat ctgcatctgt
    35821 tcctcggcct acgatccgtg attttaattc cgctaaatct ggcggagtca aaaagatgaa
    35881 tacaccatcg ggcacttttt ctttgacttg ttttgctcct tggacttcaa tctccaagaa
    35941 aacatctttt ccttcatcca atgtcttgtt gacatatgac aaaggagttc cataataatt
    36001 cccgacatat tctgcgtatt ccagcatctc tccagcttcg atcattgatt caaactcttc
    36061 tttgcttcgg aagaaataat caacgccttc gacctcccct tcacgcattt tacgggtagt
    36121 cattgagatc gaatactgaa aatcattatc ctcactctca aaaattgctt ttcgtactgt
    36181 gccttttcct acaccggatg gaccagacaa cacgattaat aatccacgct ctgacatgac
    36241 gacgtccttt caactagtct ttttgtactt atcgtttatt ttgcactttt tttctgcaac
    36301 tttcaaggtt atctttaatt atagctagaa attggtaaaa aagctaccct gtaccaagag
    36361 gtcgtaaaaa agctgtcctg catcaagtaa taagaacagc caaacataaa cagcttctca
    36421 tgtttttcgt ttggttgggg ttattatcgc agatgcaagc ttttgaacgc cgtgtataag
    36481 aggtcgtgaa gaagccgatt agtttcgagt atgaagaaaa attttgtgaa aatagtttct
    36541 cacatttttg cataaatttg gcttaatacc gagaaaatgg tttttgaaca tcacttattc
    36601 aaaataaaaa aagattgtga cacgaatttt gtcacaacct ttaaatgtat tcctagagca
    36661 aagaagctaa gcgaagaaat ccaattctgt ttatttattg tatttctttg ttcagcatcc
    36721 ttgctaaaag cccatattgg acaaagtatt cactacaccc atgatctcat cttttcgaga
    36781 acgtgctttt tctgtcggat cttctacctc ttctgtgtca attcgaacaa taaatttccg
    36841 attcatcgtc acgtaagcac cgtcgttgat caaaataaac agttcgttct cattatagtc
    36901 tgcacaggca tctttccata cgtcaaagcc gtttttttca gaagggatat cccgtttaac
    36961 cacagttttt ccacttaatt cgtggaacgt tacgtcataa tatttcatta tctctacctc
    37021 tttcgtcatt caatattttg tacttgttct ctgatttttt ctaagatcgt cttagactgg
    37081 acgacttgat ttttaatttc gattgcattt gatttggaac cgatcgtgtt gatttcccga
    37141 ttcatttctt ggatcaggaa atctaattcc cgacctacag gacattgtgc ttcgataagt
    37201 tcatctagtt tttgtacatg gatcgccaga cgatccagtt cctcgtgaat atctccgcgt
    37261 tccaacaaga tggctagctc cgtcaacaac cgttgctcat cgactttcgc tccaagaaat
    37321 tcctccagct ttttttgata ccttatttga aattcctttt catagatttc tacaaaacta
    37381 tcgagttttt ttagtacttc tttgaattct tcagtattct ttttcaacac taaagctaaa
    37441 gcttgacctt ctttttctcg actcgcctca atagaatcaa gtgcgctttt caatgtatct
    37501 agagctaact gtgccaattc ttccatgctc tcttccggtt tttcttctac cgttacgaag
    37561 ttttcgtcca gcacgaagcg ttccgccatc cgactaattg acacttgatt ttgactaccg
    37621 tatcgttgtt gaattccgtc tgtcaattga gtgataagtg aatcaatcaa ttcccagttg
    37681 atgacgatct gtttttcgtt tttgccaata taagctagat tcacaaatac ttcgattcga
    37741 ccgcggctta actgctgttt aataagctga cgaaaatcgt tttcaacaaa gttcaggatt
    37801 ttaggacttc gtatttgaac atctagaaaa cgctggttga cactttttat ttcaatatct
    37861 aactgatacg cttttgtttc gcggatggct ttgccaaaac cagtcatact tttcattcga
    37921 cagggtcctt cctcattaca tattttctat tgctttttgc agttgttcaa actcttcgtt
    37981 cgtcaatgtc aggcctttcc ccattttttc atggtctggc gcccaatctc gaagatcgaa
    38041 ttttggcggg cgaccattcc agctgactag gttcagttct ttacgccacc ccttgttgtt
    38101 ttctgacaat atagcgattt cttctacgat ttcatatgaa aattcttgtg ccatggttgt
    38161 tcctccttga gataatataa atataggatc agtaatgtgt caactggttg cttaagcaat
    38221 tcatgctgca aacgatcctt gcgttttcct tgtagcagat aatcaaaata aacagttttc
    38281 gtgcgctcat tttgtatttt ttgctggtat tttcgatacc ttcgccattc ttcagtactt
    38341 aattgaccaa tttgtgccaa taaatcatcc ggcatacttt ttatatcttg ttggcagatc
    38401 atttgaatca tcatgtctac ttgatagatt tcattgggca gtaaaaaatg tttttttgct
    38461 aaaagttcta gcatcgccaa tccgcatgtc tctggtacga ccctgttctt atataaatta
    38521 cgcagttttt gccaaaattt tatgcttttg aaaaaagata attctaactg aatttctttt
    38581 gttaagtaag acaaaaatcc tcgccagtat gttcttgcag gtttaacaga atgaaatgta
    38641 taccagttcc catagtaata gcctaatcgt tttttcattt ccagatagcc taattgtaaa
    38701 tttaattggt tcctttggct atcaaaaagc agaaagctgc ctagtgtcca caatgtttga
    38761 catttccaat ggataaaaat gtctggcata cgaatttttt tcgctgggcc tggcccttgc
    38821 acatctacga caaatgcctc tgttgctcct ttgttaatcg caatatcaat aggtattttg
    38881 ttgcgatacc caccgtctgc atatttattt tttccgatct ttcgataagc catagcggga
    38941 taaaatgaag cagaggcgag tatccaatct ggtatttctt ctgtttttgt ctgttggata
    39001 tcagtcacta cttccgctag ttctggcagt ttagtagaca ccgtatataa acgtattttg
    39061 ggattatgtt tgagtaagga caaatccaaa ttgtctttga ccatcttttc caatggagca
    39121 gaagaaaccc cgccttcaat tagtgctgtt ttcgtcattt ggcgtctttc tcgatagaag
    39181 cgttcacgaa ggtcttcaac tgcagccatt tctggtaacg caagcacttg tcttgtcgac
    39241 agctttttcc ataaattcac agcttttttc atatctcctt gtaaaatcaa tgcgccgttc
    39301 aacgcaccta ccgaggtacc tgtgataatc tcaaagttga tatcatgttc cttcagcgcc
    39361 tgccacacac caatctgata agcaccgtgc gctcctcctc cacctaaaac aagtgctgta
    39421 tgatagacca gctttttatt taattgtgtg gttccttgat agccgttttt atgaaaaata
    39481 cgcaaccatt cttctgatag aggagattga aataaaaaac aacagttttc tttaataaaa
    39541 aaacgagcgc aagcttccat ataccgaaaa aattttttcc attcaaaagt ttcaccttca
    39601 atcaacagat tattaaaaat aacatctcct ctacttttag caatagtaaa atagacggta
    39661 tattttttcg atactgcttt aaaagcacat tgcttttttg cagaacgcca aaaagcgtct
    39721 ctactttctt gatgactttg aaaaaatgga aggggttgtt ttgcttgctg gataggcgaa
    39781 tacagcgaca ttttctgtga aaagtaaact ttttttatct ccatcgcgct ctcctttttc
    39841 ttcaagtata ccagtacgtc ataaaaaggt catgagtctc gtattcgact ttgtagattc
    39901 cataagaaaa caattcataa aacctccttt aaataaaaaa atacaaaaat cacaaaaatt
    39961 aattgaaaat gagagtgttt tcaatgaaaa cgtgattttg taatggaatt ttaaaataaa
    40021 agaaacaagc ttttttaatg caaatgttgt aaaataaaag agattaagag gaaataagag
    40081 gaggaacatg atgactgagt cagtttatgt acatattgat accacaagta atgctgtttt
    40141 aacaaaaggg ctgactgcta ctgactttgc aaacagtatc gtgcattttc ctcaaaactt
    40201 gctcttgctt gatccatctg cttctgctgg agaatatgaa tcccatacag ggctgaaagt
    40261 aatccgtggt acagaaaata ttcagcgttt cttttcctct tctcgtaaca ggcgtggatt
    40321 cgatgaattg aagtggatcg actttactga tttaacgatg ctgaaagaat tgacaccttt
    40381 agaaatttca gaattattat attttggtca tatgaaaaca catcttcatt cgccgttttt
    40441 ttataaacta cagaataatt ttgtatactt cgatcttaat gatcagctga atcggattta
    40501 ttatcgctat ttagatgaat tttataggat ttttgctagt aaattaagtt cgatcttgtc
    40561 agataagatc aatgtaaaaa aatctttttt caaaaaagaa acacctgttg aaaaaatcag
    40621 tgtggaatta ttgaaagaaa tgcgtaattt gatgcaggaa gggatcattt tttcttttga
    40681 tcaagttggt ttgatcgatg gtaaatatcg tatcccaatc tatgtgattg aagattatat
    40741 ttggaaatta aaaggcaccc ggtatcgaga agaggaaatc atcgctactt taatctactc
    40801 tgtcaccgat cgacactggc aattggtaga agatgccgat ctttcttatc tgccattgaa
    40861 gtagatctat ttcttatcaa aaaataaaaa ggaaacaaga aactaggaca tgattctgtc
    40921 tcaagtttct tgcttctttt ttatttacag ctcctgtcgt ttatctggca caaaaatcga
    40981 aggagtcgtt tgttggatcc ctaataaaac gatgatcgtt acaatagctg tactcaaccc
    41041 gctggcacca ttcattacga aagaaaagag ccaaggattc atcccccata gtgcatacgc
    41101 tccccagaaa accactcctg caataaaatg ccaaaaatat ctggaaagtg cgccaatcaa
    41161 tgcaccaaac acgatgcttt ttctaatttg tttcgtatta tcgtctacat agcctttttg
    41221 gacatttgaa gccatgagcc ctgctgcgcc tgcaaaagta taggcaatcg ggtactcaat
    41281 caacacttga acgacactta aataataaac ttgtcccaac ggaaaatgta ggatgcccca
    41341 aatgaaacct gccagcatcc ctgcctttgt ccctcttctt aacgcaaaaa gagtcaatgg
    41401 aatctgccct aatgaaatag aaaaactgct gccgatctgg atcggaatca acgacaacac
    41461 catcgctaac gctgcaacaa tcgttccttc tacccaaact ctattttttc ccataaaaaa
    41521 acttcctcct ttgaataaaa aagtcacaaa ggaggaagca gctttcgaaa atagtcaaac
    41581 agctgtttat tcacaattcc tacgctcgca taaccgatac aggtacgaag ggtttagaaa
    41641 tagattcaat ctcagcccaa ttggactccc ctttgtgagt ttatttatta tttatttttt
    41701 gtttcaaaca atggactaac tggtttgttt tcatggatac gtttgattgc atctcccatt
    41761 agtccgccaa cacttacttc atcgattttg tcgattttac gatcttctgg aagatagatc
    41821 gagtctgtaa caactagacg ctcgatagct gaatcttcta ttctttgtaa agcagggccg
    41881 gataatactg gatgtgtaca tgaagcatac acactagttg caccagcttc tttcaaagca
    41941 ttggcagcca atgtgatcgt acctgctgta tcgatcatat catcgatcag tacacaagtt
    42001 ttgcctttga cttgtccaat gatattcatt acttctgcaa catttgcttt tggacggcgt
    42061 ttgtctatga tcgcaatcgg agatttcaaa aattccgcta atttacgtgc ccgtgtgaca
    42121 ccaccatggt ctggtgaaac aacgacaaca tcatcgcctt tgattccttt ttctaagaaa
    42181 taattggcaa taagtggcgc acccatcaaa tgatcgactg gaatatcaaa gaaaccttgg
    42241 atttggactg catgaaggtc caaagtcagc attcttgttg caccagcttt ttgaatcatg
    42301 tttgctacta atttcgctgt gattggttcc cgtgcacgtg ctttacggtc ttgacgcgca
    42361 tagccataat aaggcatgac tacgttgatt gtttttgcac ttgctcgttt cagtgcgtca
    42421 atcatgatca gcaactccat caagttatca ttgactgggc tgctcgttga ttgaataaca
    42481 tagacatgcg atccacgaat actttcttca atgtttactt gaatttctcc atcactaaat
    42541 tgagtaacag atgattttcc taactcgacg ccgactgctg ctgcaatttt ctctgctaat
    42601 gggcgattgg aatttaacgc aaaaattttt aatcttgggt caaagtaatg ttttgacatg
    42661 gggacctcca ctttcttttt cacaactcta gaattctacc taataaatag tagtcatatt
    42721 ttttaaataa atcaagtgat tttgcaaaag tcttagttta gataagggag tttttttgca
    42781 tatccttctt tattgacttg tcttgctctc gcaattgcca gcgcatattc tggaatattg
    42841 tctgtaattg ttgacccggc agcaatcgaa ctgtttttag ctacctcgac aggtccgatg
    42901 atattcgtgc tagaaccaat aaagctgtgg tcgccgacag tcgtatgatg tttgttcttt
    42961 ccatcatagt tgacgaagac aacaccacat ccgacattga tttcttctcc cagcgtagca
    43021 tcaccaacat aagtcaagtg accgacttta gttcctttgc cgatttgagc atttttcact
    43081 tccacaaagt ttccgatatg gacgttttcg ccaatttcag cttttggtcg taaatgtgca
    43141 taaggtccca catctgcatg tttcttgaca tggctgcttt caatgactga gttttcaatg
    43201 acgacgtgat cctcaatcct actgtctacg atttttgaat gtgacccgat gacacaatca
    43261 gaaccaatga ctgtatttcc ttggatttgt acacctgctt cgatcaatgt gtctggaccg
    43321 atttctacgc ctgcatcgat gtatgtcgtt gctgaatcga caaagcttac gccattgacc
    43381 atatgcatct ggttaattcg ctttctcatg atctcatttg ctttagctaa cgcgatccga
    43441 tcattgactc ccattgattc ttcaaaatca tccgtttgat aagcagcaac tgtatggcct
    43501 tcttctttta agatttcgat gatatctgtt agatagtatt caccttgagc attgtttgtt
    43561 ccaactttgc ttaacgcatc gaacaaagct tcgttgtcaa aacagtaagt tcctgtattg
    43621 atttcttgta caagagcttc ttctggtgtt gcatcttttt gttccacgat tttttctacg
    43681 atcccaatat ggtcacggat gattcgtcca tatcctgttg ggttttctgc ttgtgccgtc
    43741 aagatcgttg cacttgcatt ttttccttgg tgatattcaa aaagattgtt cagcgtttca
    43801 gtagtcaata atggtgtatc gccactgata actaacgttg ttcctttttt tccttccaag
    43861 aaagaagcag cttgcaaaac agcatgtcct gttcccaatt gttctgcttg taacgcatat
    43921 ttactgcgtt ctcctagttg tgctttaact tgttctgctc cgtgtccaac gatcgtaatc
    43981 acttcatctg gtttcgtttc actgacccga ttaatgatat gttctaccat aggtttacca
    44041 gaaacgggat gtaaaacttt atataatttt gacttcatgc gggtaccttt ccctgccgcc
    44101 aaaataattg cataacgtgc gtccaactgc gtcactccta aatatagttt gctgatttca
    44161 gtttatccca gtatacccat tttttcaaca aaacaagtga ttttctttta tcccagcgtt
    44221 ttctggataa aaaaatagat agaccataac gggcctatct atttggtaac cggtgctctc
    44281 ctgccaactc ctcggtcaca agtcacacta ctcataaaac atgaaaagct gttttttgaa
    44341 atcgtttctt gtgacttaga gttaaacggt aggcttacaa cctcttcatt aaacgttttt
    44401 caatcagctg accttcggta ttagctcaat aaacggcaaa atatgaggag caatttttcc
    44461 gtttattgag ctaatactca ggacaggacg ctgatttcac aacctctaga taaacggtgt
    44521 tcaaaagcca gtttctcggt attaagccaa atttatgaaa aaatgtggaa agccattttc
    44581 acaaaatttt tcttactact cgaaactaag cggctttttc acaacctctt attctgaaaa
    44641 ataatttcct ggcacaaccg taatattttt cgtttgagta tctacatctt tgacataaag
    44701 cagtgacgta taatcatcga tggcgcgatg accgttgaat tttgattcgg caaatacagt
    44761 gatgccaaca agttctgatt cgaattcttc aatcaagctc ttcattccgt tgactgttcc
    44821 gccgcctttc atgaaatcat ccactacaag gacacgtgaa ccacgtttca aacttctctt
    44881 agacagttcc atcttttcaa ttctttcaga agatccagac acatagttga cacttactgt
    44941 tgatccttct gttatttttg agtcccggcg tacaataacg aatggcgcat tcagataata
    45001 ggacacagct tgggcaatag gtacgccttt tgtggctaca gtcattacag catcgatttt
    45061 ttcaccaagg tatttagaag caatgattct tccaacttga cgcagcagtt caggttctcc
    45121 taacaaatca gagagataaa catatcctcc tggaagcaga cggtcttgtt ctgatagacg
    45181 ttctgctaga gattcaatat attttttcgc ctcttcatat gaaatttcag ggatgaacaa
    45241 tacaccacct gcagcacccg ggattgtttc caaggttcca taccctcgtt ctttaaatgt
    45301 tttcttgatg attcccaaat cttcactgat agaagattta gcagattcat aacgttcggc
    45361 aaagcttgtt aaggaaatca attcatgcgg gtgattcaat aaatactgtg tcatgtcaac
    45421 tagacgttca ctgcgacgta ctttcaccta ttccaactcc ttttaagaaa ttttatcgtt
    45481 ttacattata taagtttttc ttcaaaaatt cgagcatttt atgaaaaaat aagaaaatag
    45541 ttcggtattt attaaaataa aaagaagcct ataaaaaatt tttcgttttt ataaggcttc
    45601 ttttctttat aatttagtta ttttttcttc tgaaggcaac caatattcgt ttgatgatat
    45661 ttacaatcag gaacaaagca ataaagacta atgtgattgt tgctcctgga ggtgtatcta
    45721 gataaaacga gctagttagt cctgacagca tccctagaaa tcctacacaa acactgatag
    45781 caatcacgac attgaagctt ttgcctaatc gcataccgat tgctgcggga agaaccatga
    45841 ttgcggatat caaaagtgca ccggcaatcg ggatcatcac agcaatagcc acaccagtaa
    45901 tcacattgaa catcatcgac atcaaatgaa cgggtagccc atccacgtgg gcggtatctt
    45961 catcaaaagt cagaacatac atcggccgtt tgaataaaaa gaacaatata ccgatggcaa
    46021 cgaataagat tcctaaaaag atgacttgct cccatgtaat ggtcacgatc gaaccaaaca
    46081 gataggattg gatacttgtt gctgaattcc ctccgctcaa attcatcaat actaacgcta
    46141 gcgccaaacc accagccatt aaaatggcaa tggagatttc tgaataggtt cggtagatcg
    46201 ttcgcagata ctccaatatc acagctgcta atacaacgac tagcatggtc gtaagtgtag
    46261 gattaaggtt caaaaagaat cctaacgcta cccctgctaa agagacatga gaaagcgtat
    46321 cagccatcag cgattgtctt ctgatcacta aaaacacacc tagcatcggg gcgattccgg
    46381 caatgaagat agcggcaaga aacgctcttc tcatgaattc atatgaaagc aacgccattg
    46441 agaatcctcc tttctcacca gtcggatctg tctgtccgca tactgtttga tatcttcatg
    46501 atcatgggtg atcattagaa ctgatttccc atggacatgc gcactatgtc ggagaagacg
    46561 ataaaattca tttctggatt cttcatccat acctgttgtt ggttcatcaa gaacaaataa
    46621 gtcagggtca gtagcaaaaa tacgcgctaa actgattcgc tgcttttgtc cgccagacaa
    46681 ttcacctatt cgccgatttc gcatatccca catccctaca gcttctaaag cacgctgtac
    46741 atgttgatga tctttttcag agagacgctt gaaccagcgg cctcttggat atcttcctga
    46801 acggactaat tcgatcaccg tactaggaaa acctgcattg aaagatgcaa cttgttgagg
    46861 aatataaccg atacttaatt tttttccttc ttgatttttc tcagcaatct ttatcattcc
    46921 ttttgaaggt ttcagtaatc ctaacgatgc tttcaccaag gttgacttgg ctgctccatt
    46981 ttctccagtt aaaatgacaa attcacccgc atctacatga taagagacat cttctagtac
    47041 aggttcatca tcatagtaaa aggtcaaatc agatatttcg atataacgca ccttacaacc
    47101 tccattctaa aacttaattg atactttctt ttaaagcttt cagatttttt tccatgacag
    47161 aaatataatt ttcgcctgct ttgatttgtt cattagtcaa gctttctaaa ggattcaaca
    47221 cttctagctt cactcctgtt tcattcgaca atgtctcagc taccttcgaa gaggcatttt
    47281 cttcaaaata aatcactttg atgttgtttt tcttcacaaa ttcttttagc tcagctaagc
    47341 gacttggtgt gggttcctga tctggtgaaa cgcccgaaat cgctacttgt tttaagtcgt
    47401 attgattcgc taaataagca aaagcagcat gctgagtgac aaatgtgcgg tttgttgcat
    47461 cttttagctc atcggagtat ttttgatcca gtgcttcaag ctttttgata taagcatcgg
    47521 cattcttcgt aaaagtttcc tttttatctg gatatttttc gcctaattct tcagcaatcg
    47581 ttctgacttc ttttatagct aataccggat cgagccaaac atgtggatcg tattcatgtg
    47641 aatgaccatg cccttcttct gattcttcat gttcgtctgc ttcttgcgat tctaaacggt
    47701 cgattccttt agctgcttcg atcatcaagg tctgtttgct atccactgaa tcctttaacg
    47761 aatccacaaa aaattccatg tctgaactat tatacacgaa aacatctgca tcggaaattt
    47821 ctgctctttc ttttgcactt ggttcgaaat catgcggctc cgttccagca gggatcaaaa
    47881 gtttcacgtt tccttcatcc cctacgatct cttttgtaaa atcatacatc ggataaaaag
    47941 tcgtaacgat cgtcaaatcc tcttttttgt ctgcttcctt gttcgtattt ccacatgctg
    48001 caaacagtaa agcacctatc attaccgtag ctgtcaaaag atattttttc atttatatgc
    48061 tcctttcaat gaacaaatcg gaattttacg atttattaac gtgaactaat gtatcaaaat
    48121 aaaaaaacac agtcaataga aatccatcaa tttctgattt aagcatgtat attcttgatt
    48181 aaatcgtaaa atggatgagt tgctgctcat atctagccat ttcactacaa taaaaaactg
    48241 aagaatacca ttttaggatt cctcagtttt tcatcaccta ttttacttct tatattttat
    48301 tgcagggttc gtactaaata aacttcctca caaaaacctt ttaacccatt atatactctc
    48361 tgcgctcttg aataatgttg acacaaggca aaaacggttg gtccgcttcc actcatcaat
    48421 gcagcatctg ctccgtactt gatcatacgc tctttgattt gatggatgac tggatgccgc
    48481 tttcccgtta tatcttctaa actgtttccc atttttttga tcatctgctg ataatcttgc
    48541 tctcggatgg cttcagcaag tccgtggatg tcttgatgtt ggatggcttg cgtatctact
    48601 tctctaaaca ctgtacgtgt agagacactc attcttggtt tgaccaacac aacccagcac
    48661 tgtggcatag aaggtaatac ttggatttct tcccctttgc caccgacaaa tgccgtactt
    48721 ccgtagacac agtaaggaac atctgtaccg acttccaaac cgatttctgc cagttcttct
    48781 aacgaacagc ccagattcca taattggttg atccctctta gagcagctgc acagtctgta
    48841 cttcctccgc ctaatccagc tgctactgga atattcttct taacaaagat cttgacaccg
    48901 gttttgatat tgtatttatt ttttagcaat aaagcagctt gataaacatt gttccgttca
    48961 tctaccggta aaaatgcttt atttgtttct acaatgattt tgtcttctgt tatttcttca
    49021 aaaaatagat gatctgccaa gtctacgctc gacatcacca tttccaactc atgataccca
    49081 tcttgccgtc tatataatac atccagtcct aaatttatct tggctggcgc tttttcaatc
    49141 aattccatcc tctcacccat tcgaaaagtc taagtcgcaa gtattctatc atagggaaaa
    49201 ataaaagtat agcttatgtc tgcctagtgg ttgattttgt catcgtttca acagctttca
    49261 cagaattttc tcttcataat ccaaacaagt tctagtgatt cttataattc tgtgagcact
    49321 tcttctgcca aactcggaac atttcctttt tcttttccta tcagactgag tagctgcatg
    49381 gtagcaaatc cgaaattact tcctttccta taagcaaaat aacgtccttc ccaatgatcg
    49441 acatatttta atttcttttc ataagcagac tggaaggaat agaaaaaatt accataatta
    49501 tatacgatat gcatgacttt ttcttgaaag aaagaaaaat ctgtttctcc aacattagct
    49561 aatggagcta agcctagatt ggcttccgag tagcccattt tctcgtatac aataaacaga
    49621 ttggctaaca gatagtcagc taattcctca gattctttag gtaagacccg caacaaatca
    49681 tatcccgcct gcttcttcgt catcggctga gcggtgatga agccaacgac tttttgatct
    49741 ttacggacaa taccgacacc gctagataat acatatgatt tatcaaatcg tcccacagag
    49801 aagtattttt ctcgtgcacc ttgaagccat ttttgtgaaa cttcatttac agcttgatgt
    49861 aattcttccg gcagttgatc gtaccaagta aaatggtatc cttctttcat caaacgttga
    49921 tattcaagtt gctgactcag ccgagacaag tcttctgttg ctagatcaac aatccctgct
    49981 tcacctactt tcgtgaagtg aaaacccaat tcatgaagca tcactacgta gctttctgat
    50041 atcttataaa acgcaagctg atagcctaaa caatctgctt gtttcataaa ctgattcgtt
    50101 gctgcccgca tcattttagg attgccaact ggatctccta aaacaaagca tttgtttcct
    50161 ttgatctgat aaccaaaaac gacttgatct tggtcgtctt cttggtaata aaaataactg
    50221 taacctgaca agcgaagctg atggcttgta gctgtgcctc cgtatgtttc caataaacgg
    50281 tcaaaacggt ctttttcgta ctcactgcca agcttcgtag tatcttcagc taaatattga
    50341 taaagcgcca ctaaagctag caatgagatg cccaaaccaa taagccctga gaaccacaca
    50401 tcatctgaag gaaacaaaat aaaatgattt cctgttattc gaccgactaa aaagcgtccg
    50461 ctgtgatagc ctgcaattgc gtaaacgata aacaaaaagc cgaacaagct agcatcaaag
    50521 attatagctc cccaagaata aacgaatttt gttcgataaa attcttttct ggataaccac
    50581 acagctaaca gaatcaagac atataccaat atcaactgcc aagataacgt acgtgtgatt
    50641 gtatttaaaa taccaaaaga tagtaaagca atcgtaggca gaaatgcctt tttgactttt
    50701 actgaaagtg ctctagcaag gccgagtaat aaaaagccaa ccaatagatt cactgtctga
    50761 tctaaaaaat tgaatgagaa cggcaataat acttgaaata gccgactgat tgcagacaga
    50821 ttagtgattg ttgatagaag taccatcatg atccccgcaa aatagacagc acttacgagc
    50881 acgatatgcg caaccttttg gaaaaacagt cgcggaatgt tatctaaaaa tcgattgatt
    50941 ttgactcctg tatgtgtgat gaacaataga agtcccgtca taaaaggtag aacataataa
    51001 aacaaccgat aatataatag ccaaactact gctacatctt gccgaacaga caattgactc
    51061 attcctaaga tcatcaaaac gtcaaaagtc cccattccac ctgggaccat cgtcagcatt
    51121 cctatcaatg tagcaatcat gaacatagga tagatcgaaa gcatcgaaat atctattctc
    51181 atcaacttgc cgatgatcaa aaataccgca agggctcccg tccattgtcc aaaggatgcc
    51241 agtagcagac caagcaagcc ttttggataa aactcgaaga acaatgtctt tctctttaga
    51301 taagcaaaaa agaaaatgac tggtgctaaa aaactaccgc cgaaaagcca gatccaatag
    51361 cttcgaaaaa tactttctga ctgaataaaa aagacatcaa taaatgtaag aaacgaccat
    51421 acagacaaac cagtcagcat aaatagtgcg acttttgata cagtggctaa aaccattttt
    51481 ctatttgtct gctttccata aaaactagcc cgcagacttg ctcctaccac accaccaaaa
    51541 ccggcgagat tgttgatcgt gtttgttacc cacgcagcaa gaagaaactc ttttctttcc
    51601 atttttggtt ttccttgttt ttctaagact ctgatcgtca cccaatcata caacaacatc
    51661 ggagtgacac ccagcagtcc agccagaatc atgagcagga tcgtcatgcg gctttgttgt
    51721 cccatccttt gccaaatatc ttgccaactc atgccattag caatatttgc gacttgattt
    51781 gccacaaaga ctaacacgga gccgaaaaaa atcagtttta aaatcagact atgttccttt
    51841 atccattgca gcatcaagta tcgtttattt ttcacatttt cccattccta acttgtatac
    51901 ttttgtgtca taccccaatg ttatcttatt tattttaatt tttccacttt agacctgttc
    51961 tttgcttgcg atgatagaaa aagaaagata tcctaatgct aaaggagagg ataacatgaa
    52021 agtaggattt atcggcacag ggaatatggc acaagctatt attgatggtt tattaaaaaa
    52081 aggaacagtc tcgaaagaag aggtgttcgt ttccagtgga cattttgaaa atgcacaaaa
    52141 atttgctgct gaaactggcg tccatgcatg caaaagtaat caggaagttg catcacaatc
    52201 agatatcgtt cttttagccg tgaaaccaaa aatcattgaa aaagtattgg aagaactaag
    52261 agaatatagc cagaaagtct tattcatttc tattgcttca ggaaaatcat taatagattt
    52321 agaaacagcg cttgaagatc ctcaggcctc catcgtacgt gtgatgccga atgtcaatgt
    52381 cagcgtagga gcaggcgttt ctgcgatttg caaaaatgaa gctgccacgg aggaacagct
    52441 tcagcaagct acgatgattt ttgaagcaat cggcagcgtc tatcctttag cagaatcaga
    52501 tttcagtaca ttcattggcc ttgctggcag ctcacccgca ttcgtttaca tgctgatcga
    52561 tgcgatggga cggactggtg tactgaatgg tttgtccaaa cagacagcca ctgagattgc
    52621 tgcaaaagca attatgggtt cttgtcaaaa attcctttca agtgataaaa atccatggga
    52681 acttgtagac caagtttctt ctcctggcgg tacaactgtt gcaggtgtag ttgccttaga
    52741 agaagcagga ttcatcccag caatcatcaa aggaatcaat acgacgatcg agaaagatct
    52801 tgagatgttg aataaaaaat aatgatagca atgttttttc gtgccttaca aaaaaagaag
    52861 agctgtggct tttgccgcgg ctcttctttt gtatttttta ttatttagca taatcagttg
    52921 cacgagtttc ccgaataacg attactttga tgtgtccagg ataatccagt tcttcttcga
    52981 ttcgtttacg aatatcacgt actaaacgaa cagcgtctaa atccgagatt tcttctggct
    53041 tgaccattac ccgaacttcg cgtccagctt gaactgcgta acttgattct acaccggcga
    53101 agctgttcga aatattttcc agacttctta aacgacggat gtagttttct aatgattcac
    53161 ttcgcgcacc aggtcttgct gctgagagcg catctgctgc agctacaagg acagagataa
    53221 cagaagtagc ttccacatct ccatgatgag aagcgatcgc gttgattact actgggtttt
    53281 ctttgtactt ggcagctaat tccgcaccga tctcaacgtg agatccttca atctcatggt
    53341 caagtgcttt cccaatatca tggagcaacc cggcacgttt tgctaattgg atatcttcgc
    53401 caagctctgc tgctaagata ccagctaatt ttgcaacctc gaccgaatgc ttcaagacgt
    53461 tttgtccata actagttctg aatcgcagac gtccgatgat cttgattaga tctggatgaa
    53521 gtgtatgagc accgacttca aacgctgcct cttccccgta ttcgcgaata cgttcatcca
    53581 tttctttacg agatttctcg accatttctt cgatacgcgc tggatggatt cgtccatctt
    53641 gtatcagttt ttccaaggtc atccgtgcga tttctcttcg gattggatcg aatccggaaa
    53701 gaacaactgc ttccggtgta tcatcgatga ttaaatcgat accagtcagc gtctcaagcg
    53761 ttcgaatatt acgaccttct cgtccaatga tacgaccttt catttcatca ttcggaaggg
    53821 taacgactga aacagtcgtt tctgaaactt gatctgctgc gcagcgttga atcgctaatg
    53881 ataataagtt tttcgctttg cgatctgctt cttctttggc acgctgttca gattctttta
    53941 ccattagagt taactcatga ttcagctctt cttcagtcga tttcatgatg acatctcgtg
    54001 cgtcctcttt tgataaacca gcaatttttt ctagttcttt ttgttgatct tcaatcattt
    54061 tttcaacgtc tttttctcgc tcttcaatta attgctgttt tgaactaagt gcttcttctt
    54121 tactttcaag tgagttctcg cgtttttcta acgagtcatc tttacgattc agcgtttgtt
    54181 cgcgttgaat caaacgattc tcttgagatt ttaattctaa cttactttct ctcagctcac
    54241 tttcaacttc agaacggtat ttctgatttt cttccttagc ttctaacaat gcttcttttt
    54301 ttagagtttc tgcttctttt tttgcactat cgatgattcc agcagctgat gattgtgcac
    54361 cagctatttc tttctcgtga cgtgactttg cgaccataaa ccctaaacca agaccgacaa
    54421 ttaaaccgat gatagcgagg agaatgttga ataccataaa atccacctcc atactatctt
    54481 tttcattttt atcatctata cagcttctat gataaattat tctaaactcg tcaatatgca
    54541 tcttcgcata tatgtgtaca aaaaacatgc acaactttat tctaatgttt gtaccccgta
    54601 gtgtcaactt taaataaaca aagaaacggc tttctactta ttcattaaac gatggttttt
    54661 aaaataaatg acactagttt catataggta aacctttaaa ttgagaaact tttctattaa
    54721 attattcttt gtagatgaac agatattttt attttatgta cccactcgta taattaaata
    54781 actcacgatt tctttattca ataaaaaaac ggcaggtcaa ggagagtacc tactcttcag
    54841 accagccgtt tttgtttatc ataattttta ttcttctact aaaggcaatt cttcttgtcc
    54901 tttttcatct tctgggacag cgacttcttc accaatacca taagctgctc taactagagc
    54961 agaaacttct gccatcattt caggatgatt agccatatag attttagcgt tttcccgtcc
    55021 ttgaccaatc cgatcttctt tataagaata ccaagcaccg cttttatcaa caatgtcttt
    55081 ttctactgcc atgtctagaa gttctccttc ttgtgagatc cctaaaccat acatgacatc
    55141 tacttcagcg attttgaaag gaggtgctac tttgtttttt acgactttga tctttgtacg
    55201 gttaccaaca atatctgtac cttgtttcaa ttgttctgca cgacgtactt ccaaacggat
    55261 cgttgcgtag aatttcaatg cgcgtcctcc aggagtgatt tctggattcc caaacatcac
    55321 gcctactttt tcacggattt ggttgatgaa aatagcgatt gtttttgttt tgttgatcga
    55381 accagagagt ttacgcaatg cttgagacat caaacgtgct tgtaacccga catgtgagtc
    55441 acccatttcg ccgtcgattt ctgctcgtgg aactaaagca gcaactgaat cgactactac
    55501 gatatctaca gccccacttg atactaaagc atcagcgatc tctagacctt gttctcctgt
    55561 gtcaggctgt gaaagaagta gttcatcgat attcacacct aatttttgtg catattgcgg
    55621 atctaacgca tgctcagcat caatgaaagc ggccgttccg ccattttttt gtacttctgc
    55681 aatagcgtgt agtgcaactg ttgttttacc tgaactttca ggtccatata cttcgatgat
    55741 acgtccacgg gggtaaccgc cgacacctaa agcgacatct aacgcaagag agccacttgg
    55801 aattgtagag atttgttggt cgattttttc gcccaatttc ataatagagc ctttaccata
    55861 gctcttttca atttttttca gtgcagcatc taaggctgct ttacgatcat ctgccaatac
    55921 gataatcctc cttatattta caaatcagag caacatttca ttatccttat catatctttt
    55981 ttagtgaaaa aaagcaagaa aaaaccgaac aaatattcgc atttatcatt ttcttaaaag
    56041 ctgttttctc aaaagatcaa atccttgcat aaccgcatta tatctgatag cagcacgatc
    56101 tctggcaaag tgatattttt ccgaaaccac gccgtcttta gatgaaatag cgatccatac
    56161 tgttcctgca gattgacctt ctaacgtgtc cggtccagcc actccggtga aagctacact
    56221 gtaatctgtt cttgctatct cacgtgcttg aatggccatc tgctccacac attctttgct
    56281 gactgttcca tattctgcta atagctccgg atcaatctcc agaaaaccag cttttgtttg
    56341 gagcgagtag gtaacgaatc cgcctggaaa tacttcagat acaccagcaa tgtcgcctaa
    56401 tgcggcttgg aaggcgccgg ctgtcaaact ttccgctgct gttacagtct gcttgttttc
    56461 tttcagtagt tccactacga cttttgctag cgaattatca tcgccgtatc cataaaaata
    56521 ttcccctact cgttcttgga ttttttcttc taatgctaaa agagcttgat tccctgcctg
    56581 aacatcattt gttttcactg tcagtcgtaa tgtcacttca tttggtttgg catatggtgc
    56641 gatcgttgga ttgatctgtg tctcaatcag atcttttagc tcagtgacca gtcgcgattc
    56701 accaatccca taaaagcgca aaactttaga aattagtttt tcttcagaag gaaaacgttc
    56761 ctctagtaaa ggacgtactt gttccacaaa cattggtttc aactcattag gcggaccggg
    56821 aagaaggata tacgcatgtg tatctgtttg ataaaaagtc cctaaagcaa gccctgtacg
    56881 atttggtaga ggaaccccgc cttcgataat ctgggactgt tgcagattgt ttttggtcat
    56941 ttttcgatgg gttgtttcaa aatatgccaa taattttttg tatccttccg tattttgtat
    57001 gagagactta ccgaggtgag ccgcagttgc ttcttttgtc aggtcatctt ctgtcggtcc
    57061 taatccgcca cataagagta tgagttcact tcttgtttca gctagagcaa tcaactcttc
    57121 taaacgctgc tgattatctc ctacaactgt ttgataataa acttcgatac ctagatcagc
    57181 tagctgttct gacaagaaag tggcattcgt attcacgact tgtcctagta aaagttctgt
    57241 tccgacagct attatttctg ctttcattac ttcccgctcc cttatatagt atacgctgta
    57301 tttttatatt tttttattta aaaagagggc aaggagctgt gacaaaaatc ttgtcacagc
    57361 tccttgttcc aaataaacag tgttctcctg ccaaaacctc ggtcataagt cacaatatac
    57421 ataaaacatg aaaagctgtt ttttgaaatt atcccttgtg actcagagtt agacggcagg
    57481 ctcacaacct ctccactaaa cgatacttac attgatcctt tgaacacacc gctgtttttg
    57541 atgaagtaat ccacacctga atagatagta aagatcagac aagcataaag catgatttga
    57601 tctactggaa taccgatcag gctgaatggg atattattga taaacagcaa gataatagca
    57661 accatttgtg tagccgtctt gacttttccc ggccaagctg cagccatgac ttctccatgt
    57721 tcgacaagta gtaaacgcaa gcctgtgacg gctaattcac gacagacaat gatcgctact
    57781 acccaaccag gggcctttcc ttggtcaacc aacatgataa aagcagtcat aactagcatt
    57841 ttatctgcca acgggtccgc aaacttgcca aaattagtga caaggcctcg cgcacgggca
    57901 atttttccat ctaaccagtc tgtgatacta gctaaagcaa aaataactgc acctactagc
    57961 tgtgtgacag ccagtgtggt gtctcctacg ttaaattgtc cccaatctaa aggtaaacct
    58021 acaaccaaaa tgaaaatcgg gatcatacaa attctaagta cagttaattt atttggtaaa
    58081 ttcacactat cgactccttt cttccctatt ctacgtgact atcagcgaag tttccaccga
    58141 tattatttac ttttccaaat gaatttattc aaaggagtat ttttattgaa attgatcttt
    58201 ttgaattaac gaagaaggac cgcgacacag atttgtcacg atccttcttt tttataccta
    58261 caagtaaaca gatgcgtaaa ctaatttata cgatcattct gcgtattgaa gagtaaggtt
    58321 cacatttttt ggactctgat tgttttcccc tggattaact ggtacggatt ctccattcgc
    58381 tttgatcgtt gcattgcttg ctgcaccaat cgtgatggtc gcatttgttg ctgtttccgg
    58441 taacacggta ctctgtgttt catttgctgc cagtgtccct tgataaacat aggcattgtc
    58501 tactagcaca cctaaccata cacgattgac agcagtaaat tcaagggtta caggcttggt
    58561 agcgtctgtg atatttactg cgatggcact gccagaatct tctcctgtag ctattgccat
    58621 tttcttttct tcacttgatt ttgttgttga ttctactgta gaagactctt tggtctgcgt
    58681 gctttgctct gaggaagagg actcctcgac tgatccttct actttaagcg aagaagctgt
    58741 gtctccgatt attggatcag cttgacgatc ttgccaagtc atatagccca cgacaacgac
    58801 aatcgccaaa gcaaccaatc ctaataaaat gacaggaaga cttgtccaaa agcgacttgg
    58861 atgcgattct tcctgatgca ttgcttttct agaagtactt actgtttcag gctgcggacg
    58921 ttcagggacc gatcctgtca atttgctttt cccatcaaaa acatcgacta aatggtctcc
    58981 atcttctcct acagcttcag cgtattgccg aataaatgcc cgcacgtaaa atttaccagg
    59041 caattggtca aaatcatcat tgtcgattgc ttccaaataa cgtttttgaa tttttgtaat
    59101 ctgctgtaat tcatctaaag atattttttt attcaggcgt gcttgccgta atttttctcc
    59161 gatggtttca aaagccactc gttcatcttc tccagtctgt ttgattctcc gaagcatctg
    59221 ctgttgcaac gaaagacgtt atgtttgggt gctgtgacaa aagtcatgtc acagctcctt
    59281 ttttccgaat aaacattgtt ctcctgccaa ctcctcggtc acaagtcaca atatccaata
    59341 aatataagaa gctgtttttt gaacttgttc cttgtgactc ggagttaaac ggcaggctca
    59401 cgacctcttt cttaaacggt gttcaatcag ctgttcctcg gtattagctc aaaaaccggc
    59461 aaaatataag gagatatttt tccgtatgtt tctctaatac tcaggtcaaa acgctgattt
    59521 cacaacctct tccttaaacg gtgttcaaaa gcttgcatct gcggtaataa gtccaaccaa
    59581 gttaaaaaca ttaagaagct gtttttactt ggctgttctt attacttgat gcagggcagc
    59641 tttttcacaa cctcttggta cacggtgttc cagaagtagc ttcttcggca ataagccgaa
    59701 atatcacaaa aatttgagaa gcaattttcg taaattcctt cttatttctt gaagctgacc
    59761 acttctgtca caacctcttt gattacttcg tttgatttta ttttaatata agttgattaa
    59821 aagagagcat gatttgtttt attttctcac taatttttcc aacacaacta agaataccat
    59881 aaaagctgat tttggaggag gttcctacta caagtctaaa acgaaattta agacttttcc
    59941 tcataaaata ctggtttatt ttggtctcat gtaaaactcg ctgaaggcct cttcacttat
    60001 aaatgcttcc ccagctgcca gtacgtcttt catttggatc gaatcgatga tttcaggaag
    60061 atcaaataaa gttcgatcgc caaataaact ttgcgtaaat tgattggcaa tatattcgat
    60121 cgaatttaat gattggaaat attgtccaag cattttcttc ttcagcaaat cgagatttgt
    60181 ttctgacact tccggatcat cagcgaatcc aagaatgatt tctttgactt tttctgctgc
    60241 tttctctggt tcgtccgtat ctccgctgaa gtccgcaaag tgaaattcac gatccaagct
    60301 gaattcaaaa ccaaagctgt catcgataat tccttgattg tacatcgcta gatagtttcg
    60361 agaagtattg cctaaaatca tttggaacaa cagattgatt gctgttttat agcgtaacag
    60421 ttctgttcct tcttgcggca atgtgtctaa tcctttaata ccaagtacaa atttatctcg
    60481 tgtaatggct gcttccaatg cactttgttt gatgatttct tttgtgtttt cagggaaata
    60541 acgtacaatt tcttgctttg gagggaaatt ttttgcttct tggttttccc gaataagttt
    60601 catcaatttc tcaggctcca tcttaccaac tacaaataaa accatattgc ttggctgata
    60661 aaacgtccga tagcacgtat acaagtcttg agctgtgatt ttgtcgatac tttcaaccgt
    60721 gcccgcaata tcgatatgca atgggtgtgt aggatacaag ttatttagta tgccaaaaaa
    60781 catccgccag ttagggtcgt cttcatacat ttggatttcc tgaccgatga ttcctttttc
    60841 cttgtttaca gtttcttctg tgaagtaagg tgcttgtaca aaatcgatca gagtcgtcag
    60901 attcttttct acctgatccg tcgtagaaaa cagatagctg gttttcgtaa aacttgtgaa
    60961 agcattggcg gaagctcctt gtttcccaaa taattgaaag acatcaccat cttctttttc
    61021 aaacaactta tgttctaaaa aatgggcaat tccatctgga actttcacct tttctttttc
    61081 accataggga ataaattcgt tatcgatcga accataattc gtactgaaca aaccatatgt
    61141 cttgtgatag tcatttttag gcaacaaata cacggtcaaa ccgtttggca gtacttcatg
    61201 ataaagtgtt tcattgattt gctcatattc tgttttattc atttgcattt cctcctgcta
    61261 aaaagaagat tgcctgcaat tcgatttgct cagctacttg ttgtatctct ttgatcgtta
    61321 cgctctccat acgtttcaac cattcttcat cagataattt tgtttccggc agccagctgt
    61381 ccaaaaaaga agcttcgatt gccgcttgcg gagagtctag agaaagcaga tattggtttc
    61441 tcagcattgc ttttgtctga gacagttcca actctgttat ttcaccattt cgcaaacttt
    61501 ctaattgctc gtgaatcaga tgaagtactt gatttcggtt cttttcatcg ataccggttt
    61561 ggacactcat gaatcctcgg aacgtatcca cgctactaga ggcataataa gctagacttt
    61621 ctttctctct gacattcatg aaaagtttag agtgaggaaa accgccaaat aaaccgttaa
    61681 agaccattag cgcaaaacgt tctggttcat cgtaatacac atttgtctga taagctaggt
    61741 tcagctttgc ttgaacgatt ggctcacgta cttgtcgttc cttcacgata ttcacttgag
    61801 gttgggtata aaacagatct ggataaactg tttcccgcga ctcaaatggt aattgcccaa
    61861 ctgcttcagc tgctttgtcc ggatcaacat cgccgacaac aaaaatgtcg atttgatcat
    61921 ttgccatcat ttcttgatag taggacgcta gcgaactagc agtgatcttg tccaaatctt
    61981 cgactacgcc caaccctggg atcttttgtt ccgggctgtt ttcaaaataa agttcttgca
    62041 aggcatagga agcaaaagtt tgtttatctt ccttcaaact ttccaaatat agacgcagat
    62101 tgttcttttc caagtcaaac gtcaactgat caaactgatg atttttaata tttggataaa
    62161 ataaaacctc ttttaaaaaa tctaccgctt gagaaaaaag gtttggatca ttgacatatt
    62221 caccattcac aaaactgatc cctgcgttta gccaatgtaa atttcctttt tttctaactg
    62281 ataatccaaa gcttgcgcca taaagctctg caagttgcgc acttaacttt gtctgatccg
    62341 aataattcaa actatttgtt tccaacatgc tgctgagaag tgaacgtttc gtgatgactt
    62401 cttgttgcag acgcgcagta aaacggataa aaatccggat cgttttgtat tgttttgttg
    62461 gtaagacatg aagattcacg ccttctgcca attgatacac catatttata tgctcctttt
    62521 cacagttgat tgcccgctta ctcttttaat gggtcaacct gcctttttta cttattcctc
    62581 tgcttgaaat ttataagttc aggcaattcg ccgttttttc taaatgatgt tacactttat
    62641 agtatagcat agatgttttt tatcaaaaga aacagacggc taaaagcgta tattgttttt
    62701 ttatttttta tttt
//
LOCUS       DAJSBL010000011        62323 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.011, whole genome shotgun sequence.
ACCESSION   DAJSBL010000011 DAJSBL010000000
VERSION     DAJSBL010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 62323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 62323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..62323
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.011"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            591..1280
                     /locus_tag="ONT85_000806"
     CDS             591..1280
                     /locus_tag="ONT85_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR2377801.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1267..2475
                     /locus_tag="ONT85_000807"
     CDS             1267..2475
                     /locus_tag="ONT85_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HCR2377802.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2769..4040
                     /gene="serS"
                     /locus_tag="ONT85_000808"
     CDS             2769..4040
                     /gene="serS"
                     /locus_tag="ONT85_000808"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HCR2377803.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4589..6073)
                     /gene="guaB"
                     /locus_tag="ONT85_000809"
     CDS             complement(4589..6073)
                     /gene="guaB"
                     /locus_tag="ONT85_000809"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HCR2377804.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6578..7273)
                     /locus_tag="ONT85_000810"
     CDS             complement(6578..7273)
                     /locus_tag="ONT85_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HCR2377805.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7363..8463)
                     /gene="ychF"
                     /locus_tag="ONT85_000811"
     CDS             complement(7363..8463)
                     /gene="ychF"
                     /locus_tag="ONT85_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HCR2377806.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8472..8663)
                     /locus_tag="ONT85_000812"
     CDS             complement(8472..8663)
                     /locus_tag="ONT85_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HCR2377807.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8684..9574)
                     /locus_tag="ONT85_000813"
     CDS             complement(8684..9574)
                     /locus_tag="ONT85_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HCR2377808.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9561..10328)
                     /locus_tag="ONT85_000814"
     CDS             complement(9561..10328)
                     /locus_tag="ONT85_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HCR2377809.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10340..11053)
                     /gene="rsmG"
                     /locus_tag="ONT85_000815"
     CDS             complement(10340..11053)
                     /gene="rsmG"
                     /locus_tag="ONT85_000815"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HCR2377810.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(11285..13186)
                     /gene="mnmG"
                     /locus_tag="ONT85_000816"
     CDS             complement(11285..13186)
                     /gene="mnmG"
                     /locus_tag="ONT85_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HCR2377811.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(13203..14600)
                     /gene="mnmE"
                     /locus_tag="ONT85_000817"
     CDS             complement(13203..14600)
                     /gene="mnmE"
                     /locus_tag="ONT85_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HCR2377812.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14899..15282
                     /locus_tag="ONT85_000818"
     CDS             14899..15282
                     /locus_tag="ONT85_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2377813.1"
                     /translation="MVALSKLYTFERRKYVSPFRSAALEEESDPRINDVVLSTGNINQ
                     KYIVRDIVFVVECLEADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQK
                     KRDGKYFLELFAYGTVIQFIQTTIG"
     gene            complement(15450..16208)
                     /locus_tag="ONT85_000819"
     CDS             complement(15450..16208)
                     /locus_tag="ONT85_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Jag N-terminal domain-containing protein"
                     /protein_id="HCR2377814.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(16226..17044)
                     /locus_tag="ONT85_000820"
     CDS             complement(16226..17044)
                     /locus_tag="ONT85_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HCR2377815.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(17041..17397)
                     /gene="rnpA"
                     /locus_tag="ONT85_000821"
     CDS             complement(17041..17397)
                     /gene="rnpA"
                     /locus_tag="ONT85_000821"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HCR2377816.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17515..17649)
                     /gene="rpmH"
                     /locus_tag="ONT85_000822"
     CDS             complement(17515..17649)
                     /gene="rpmH"
                     /locus_tag="ONT85_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HCR2377817.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            18172..19506
                     /gene="dnaA"
                     /locus_tag="ONT85_000823"
     CDS             18172..19506
                     /gene="dnaA"
                     /locus_tag="ONT85_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HCR2377818.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            19704..20834
                     /gene="dnaN"
                     /locus_tag="ONT85_000824"
     CDS             19704..20834
                     /gene="dnaN"
                     /locus_tag="ONT85_000824"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HCR2377819.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
     gene            21063..21305
                     /gene="yaaA"
                     /locus_tag="ONT85_000825"
     CDS             21063..21305
                     /gene="yaaA"
                     /locus_tag="ONT85_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HCR2377820.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            21292..22416
                     /gene="recF"
                     /locus_tag="ONT85_000826"
     CDS             21292..22416
                     /gene="recF"
                     /locus_tag="ONT85_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HCR2377821.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQTRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            22413..24359
                     /gene="gyrB"
                     /locus_tag="ONT85_000827"
     CDS             22413..24359
                     /gene="gyrB"
                     /locus_tag="ONT85_000827"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HCR2377822.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            24379..26850
                     /gene="gyrA"
                     /locus_tag="ONT85_000828"
     CDS             24379..26850
                     /gene="gyrA"
                     /locus_tag="ONT85_000828"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HCR2377823.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDYAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKEDQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            27086..27385
                     /locus_tag="ONT85_000829"
     CDS             27086..27385
                     /locus_tag="ONT85_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005226363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HCR2377824.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            27435..27947
                     /gene="ssb"
                     /locus_tag="ONT85_000830"
     CDS             27435..27947
                     /gene="ssb"
                     /locus_tag="ONT85_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HCR2377825.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            27973..28209
                     /locus_tag="ONT85_000831"
     CDS             27973..28209
                     /locus_tag="ONT85_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HCR2377826.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            28352..30325
                     /locus_tag="ONT85_000832"
     CDS             28352..30325
                     /locus_tag="ONT85_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HCR2377827.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            30331..30783
                     /gene="rplI"
                     /locus_tag="ONT85_000833"
     CDS             30331..30783
                     /gene="rplI"
                     /locus_tag="ONT85_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HCR2377828.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            31057..32424
                     /gene="dnaB"
                     /locus_tag="ONT85_000834"
     CDS             31057..32424
                     /gene="dnaB"
                     /locus_tag="ONT85_000834"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HCR2377829.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            33204..34496
                     /locus_tag="ONT85_000835"
     CDS             33204..34496
                     /locus_tag="ONT85_000835"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HCR2377830.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
     gene            35232..36095
                     /locus_tag="ONT85_000836"
     CDS             35232..36095
                     /locus_tag="ONT85_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HCR2377831.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(36224..37846)
                     /locus_tag="ONT85_000837"
     CDS             complement(36224..37846)
                     /locus_tag="ONT85_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR2377832.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            38095..38616
                     /locus_tag="ONT85_000838"
     CDS             38095..38616
                     /locus_tag="ONT85_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HCR2377833.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(38802..40532)
                     /gene="spxB"
                     /locus_tag="ONT85_000839"
     CDS             complement(38802..40532)
                     /gene="spxB"
                     /locus_tag="ONT85_000839"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HCR2377834.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            40697..41515
                     /locus_tag="ONT85_000840"
     CDS             40697..41515
                     /locus_tag="ONT85_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HCR2377835.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(41618..41815)
                     /locus_tag="ONT85_000841"
     CDS             complement(41618..41815)
                     /locus_tag="ONT85_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2377836.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            41984..42562
                     /locus_tag="ONT85_000842"
     CDS             41984..42562
                     /locus_tag="ONT85_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HCR2377837.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            42657..42794
                     /locus_tag="ONT85_000843"
     CDS             42657..42794
                     /locus_tag="ONT85_000843"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2377838.1"
                     /translation="MDQKRIHVGLITSFVTMLIYLDQGHLTWTVVGVVGFLTLFTAIK
                     K"
     gene            complement(43607..44110)
                     /locus_tag="ONT85_000844"
     CDS             complement(43607..44110)
                     /locus_tag="ONT85_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HCR2377839.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            44469..45494
                     /locus_tag="ONT85_000845"
     CDS             44469..45494
                     /locus_tag="ONT85_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HCR2377840.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            45817..46695
                     /locus_tag="ONT85_000846"
     CDS             45817..46695
                     /locus_tag="ONT85_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5596 domain-containing protein"
                     /protein_id="HCR2377841.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            47171..47287
                     /locus_tag="ONT85_000847"
     CDS             47171..47287
                     /locus_tag="ONT85_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HCR2377842.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            48215..48553
                     /locus_tag="ONT85_000848"
     CDS             48215..48553
                     /locus_tag="ONT85_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2377843.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            48647..48877
                     /locus_tag="ONT85_000849"
     CDS             48647..48877
                     /locus_tag="ONT85_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2377844.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            49277..49648
                     /locus_tag="ONT85_000850"
     CDS             49277..49648
                     /locus_tag="ONT85_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HCR2377845.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(49922..50614)
                     /locus_tag="ONT85_000851"
     CDS             complement(49922..50614)
                     /locus_tag="ONT85_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HCR2377846.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(50617..51402)
                     /locus_tag="ONT85_000852"
     CDS             complement(50617..51402)
                     /locus_tag="ONT85_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR2377847.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            51639..53039
                     /gene="lacG"
                     /locus_tag="ONT85_000853"
     CDS             51639..53039
                     /gene="lacG"
                     /locus_tag="ONT85_000853"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HCR2377848.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            53064..53381
                     /locus_tag="ONT85_000854"
     CDS             53064..53381
                     /locus_tag="ONT85_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HCR2377849.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            53394..55067
                     /locus_tag="ONT85_000855"
     CDS             53394..55067
                     /locus_tag="ONT85_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIBC"
                     /protein_id="HCR2377850.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            55081..56712
                     /locus_tag="ONT85_000856"
     CDS             55081..56712
                     /locus_tag="ONT85_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 53 family protein"
                     /protein_id="HCR2377851.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            57584..58774
                     /locus_tag="ONT85_000857"
     CDS             57584..58774
                     /locus_tag="ONT85_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISLgar5 family transposase"
                     /protein_id="HCR2377852.1"
                     /translation="MNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKTELTAFLDY
                     EKYDRIGFNTGNSRNGSYDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYRRTNDTLEE
                     TVIHLFRKGITMSEIADLIEKMYGHYYTPQTMSNITKSFTEEVTAFNGRELHDRYAAI
                     YMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEILLDLQERG
                     LKNVLLFITDGLKGMVGAISRFYPKARFQHCCVHVSRNISHKVRVDDRKEVCDDFKMV
                     YQASSKEVALEARGTFAEKWKTSYPKVVESILSNDHLLTFYDFPLAIRKSIYSTNLIE
                     SFNKQIKKYSHRKEQFQNEESMERFLVSSFDTYNQKFLGRSHKGFQQAEGELEQMLSQ
                     LIEN"
     gene            58908..61097
                     /locus_tag="ONT85_000858"
     CDS             58908..61097
                     /locus_tag="ONT85_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR2377853.1"
                     /translation="MFISTLQIRNFRNYESEIFRFDNETNTIIGENDSGKTNALTALR
                     ILLDDSYYYSSKTLKESDFFHGIQNGWQGHWIIISATFEGISEEEFDNEICASISLNS
                     ESQTILEELISNADKGVGSISLFIRPNKAIRKQLFDISTELDSHQFNEFRNSIRLSDY
                     EFYYTSKSNLNFCINENYDKLVGRLNDNIASNPEEDDEALLGSRIDMSDIFKHISVVY
                     VDALRDVLREMKNNRNPVKRIMETIESKISSDNVESLKTIIQQLNETITSVPEIRKIG
                     ENINRQLNSIIGSVYSPNLLLSSTMSDDMGSLAKFLSMKPEQNIDLDLLGLGHLNMIY
                     LALKIVEFEACRSRELLNIMLIEEPEAHIHHHIQKTLFEGLNLQKNYTQILMTTHSVH
                     LAEVSEISRMNVLKSFNGKSIVMNPANNLNSFAKEKLKKNNLNLINAVERYLDVKRNG
                     LLFSKGVILVEGDAEEILIPQIVKKLFGINLDELGIGLINVGSTSFEYIASLFDDDRI
                     QRKCAIVTDLDEQAIPSEHRLYKSTAQEKGQARKEKLSSLFNENNWVNCFYANTTFEI
                     EFLDVNKENILTYVSPIIDDAFKDKKTITAYKEEIQSEQYLERNLAMLKLANHVGKGW
                     FAIELGEKIDFLIKIPKYILNAIAFACQEIVDLSIYKKILLHTIDLYEETTELSALSA
                     SLKTSDTADLNIAIISLLEYFGKDEDVVYLINQIENYTKILYAEVSK"
     gene            61094..>62323
                     /locus_tag="ONT85_000859"
     CDS             61094..>62323
                     /locus_tag="ONT85_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HCR2377854.1"
                     /translation="MTNIFEKIVNDLNLEQKDVVESEDNLYVNACPGSGKTRVLTRKI
                     AYQAIKHDGSLKRIIAITYTNRAAEEIKERLSLLSIDDDINIWVGTIHQFCLEFIIYP
                     FKMNLSRVSKGFTIIDDYTQRAYVSQINNMLNLNLKTYELNKIDLSLSHDFKINEVQY
                     TQVSEMYHTMLEKNKEIDFDMILLVAYKILTVNSNVARNIASNIRAIYVDEFQDTREL
                     QYNVIAKLLQNNPQIQSMFVGDIDQAIYGSLDGIAKSVEELEMLTSHTFQSKTLHGCY
                     RSNQRLVDFYSNFQSCPYEIESRGNNKNDRGFISYDCKVTKEDLPNIIKNIIKEKIES
                     GIHEKDICVLAPQHYPLFSLGEYLKKELPYCNFDAINISPIKVNNHSLFFKLAILYFT
                     ESGEKVRRRKLIANDILI"
BASE COUNT        19425 a        11323 c        12309 g        19266 t
ORIGIN      
        1 ggagtgtttc ccgattcgtt tttggctgcc gtagcaactt ttatatcata tcagagagac
       61 aagtaattgt caactctttt ttaaaagttt tttcgatttc gtttttcgaa atcttccagc
      121 aactcattta tcataactta tcgtttgtta tttgtcaaga acttttttga agtttttttc
      181 gatagaatca agcttcgcaa gtggttcttt tttaacgact cagttatctt atcatgtcat
      241 ttccaacatg tcaacaactt attttttatt atttaataag ttattggtgt tcttttgaac
      301 aaataataat atatcaacat cacatgtgaa agtcaataag aaaatgctta tttatcaaca
      361 tttctagctt acatgatcat tggttagttc tggtcctttt tcgttcgtct ttataactcc
      421 gagagacgta aaatcctttt gttttcagtt taatgttcat gttcttgtct tattcacacc
      481 aactagatgt ctcaccttct aaaattttcc ttaacaaagt gctgctttta tctatttctg
      541 cttttttttg ctagaataat agtaacaata tgactctgga ggatttattt atgaaaattt
      601 tgattgctga cgatgataaa gaaatcgttg aattattaag tatctatgtt cacaatgagg
      661 ggtatgaagc agtaaaggct tatgatggga aagaagcatt atctaaaatc agaacaatcc
      721 ctgatattga attacttatt ttagatatca tgatgcctga gatggatggg atgcaagtag
      781 tgaaagagct acgaaaagaa tcccaaatac caatcattat gctgacagca aaaactactg
      841 atatggataa gatcaaagga ctggtcgctg gtgcagatga ttatgtgacc aagccattta
      901 atccgttaga agtaatggct agagtaaagt ctttattacg gcgtacaaaa atgcagattg
      961 ccccagatca gcctgatgta ttagaagtaa atgcattaat catcaataaa gactctcatg
     1021 aagttaaaac aatcgatggg aaagaaatcc agttaacagc tttggaattc ggtatcctct
     1081 acttgcttgc tagtcatcct aatcgagtat ttagcgcgga tgaaattttt gagcgtgttt
     1141 ggaaacaaga aagtattgtc tctgctaaaa ctgtcatggt tcacgttagt catttaagag
     1201 ataagattga agaagcaact ggtggtgaaa aagtcattca aaccgtttgg ggagtgggct
     1261 ataaaattga cgcaagatag taaacgagca actgaaaaac gccgtcggat caatttgact
     1321 tcaaaagaaa tcagcgaatt gttagcggaa ggaatcatca cgattatcct tttgcttcta
     1381 ttaaatgttt ctattctggt tgttatcagt tcggtgatca acagttcacc ttctttgacg
     1441 aatgcgattt gggattcaaa gaatattttt gcagaacgat tgaacactga tttgttctgg
     1501 aacggccgga attttattat tccctttttc tttctattag atattggtgt tttgtattgg
     1561 cgcttgattc gaagatatcg ccaaatgcaa ttgcggcata tcatcagcga acttcactac
     1621 atcgcaaacg gaaattatga tcatcgtatc ccttttgaac tgagtggtga cttaagccga
     1681 gtggtaacaa gtatcaatgg attggtggat agtaccgtag cagcgattga agacgaacgt
     1741 aaaatcgaaa aatcaaaaga tgaattgatc acgaatgtca gtcatgatat ccgtacccca
     1801 ctcacgtcca tcattggata cctaggactg atagaagatg gccagtatca ctcagaagaa
     1861 gatcttttaa agtatacgca tactgcttat atcaaagcaa agcagatgaa atctttagtg
     1921 gacgacttat ttgaatacac taaggtacgc cagcctgctg tcccggtgaa tttttctgcc
     1981 tttgacatga tccagttgat cgaacagtta gcagctgatt ttgagttgga agcttctaaa
     2041 aaaaatatcc aaatacttgt tcaatcaaaa gtagactccc ttatcatgga tggcgatacc
     2101 gaaaaacttg tacgtgtctt caataatctt ttgacgaatg cgttgaaata cggaaaagga
     2161 gcaacgaaga tcgtaatcga agtagaacgt atcggttcag aagttgttgc aacagtcaaa
     2221 aataacggag caatgatccc acaacaggca atcgataatc tattcgaccg tttttatcgt
     2281 gtagaagaat ctcgttcaca agcaactgga ggaactggac ttggtttagc aatcgctcaa
     2341 agcattgtgg ctttgcacgg cggatacatt tatgcaaaat cagataagca atggacttct
     2401 ttcatcatgc accttccaat caagaaaaat gaaaaattac ctattgatac acaggaagtt
     2461 attgacgaaa gctgaaatat tcgctattct tattacaatt attagaataa atgaaaaaca
     2521 cagaacccag tacactttat tctttttcag agagctgatg gcaggtgtaa atcagcaaag
     2581 gataatttgg aagttccagt tcgtttacgt gccctttcga aataagaaag gtcggttcaa
     2641 cagccgttat gtgttcaaca agtgcagctg acgctgaact tgggtggtac cgcgaatttc
     2701 agactgattc gtcccaagaa taatgggatg gatcagtctt tttttatttt caaaaaggag
     2761 gaaatcatat gttagatgta aaaatgatgc gacaaaatat tgaagaagta caagaaaaat
     2821 tgatgactcg gggcgtaaaa aaagaaacat tagctgaatt catggaacta gatgaaaatc
     2881 gtcgtaagct actagtcaaa agtgaagaat taaaaaaata tcgaaatgat gtttctgctg
     2941 agatcgccca attgaagcgt gcaaaagaag atgcttctgc taaaatcgct gaaatgaaag
     3001 aagtcggtgg caacatcaaa gcattggata ctgaaattgc tgaagtggat gaaaaattga
     3061 aacaaattgc aactactttg ccaaaccttc cccatgattc tgtgccagtt ggaacagatg
     3121 aagaggacaa tgtagaaatc cgtcgttgga gcgaaccacg tacatttgct tttgaaccaa
     3181 aaccacactg ggaaattgca gaaaatcttg atattttaga ttttgaacgt ggcgcaaaag
     3241 tagccggtag ccgttttgtc tattacaaag gattaggtgc tcgattagaa cgtgctgtct
     3301 acaacttcat gcttgatcag catatttacg aacacggcta tactgaaatg atcactcctt
     3361 atatcgtcaa cagtaaagcg atgtttggta cgggacaatt tccaaaattc aaagaagatg
     3421 ttttccaatt ggagaatact gatctaacat taatcccaac agctgaagta ccattaacta
     3481 actattataa tgatgaaatt ttagatggtg cagatttacc tatttatttc actgcactaa
     3541 gccctgcttt tcgttctgaa gccggaagtg ctggtcggga tacacgagga ctgattcgtc
     3601 ttcaccaatt caataaagta gaaatggtca aattttcgga tgtagagcat tcttacgaag
     3661 aattggaaaa aatgacagct gatgcagaag atatcttgaa aaaattgcat ttgccttatc
     3721 gtgtgatggc cttatcaact ggagacatgg gcttttctgc agctaaaact tatgacttag
     3781 aagtatggat acctgcacaa gatacttatc gtgaaatcag ttcttgttct aactgtgaag
     3841 atttccaagc acgccgcgca atgatccgtt atcgcgataa tgatggaaaa atccactata
     3901 ctcatactct gaatggttca ggacttgcag ttggccgtac agtagctgcc attttggaaa
     3961 actaccaaaa tgaagatggt tctgtcacag ttccagaagc gctcgttcct tatatgggga
     4021 atctgaaagt tattaaataa agaggctgtg aaaaagccgc ttagtttcga gtattaagaa
     4081 aaattttgtg aaaatggctt tccacatttt tgcataaatt ggacttaata ccgagaaact
     4141 ggcttttgaa caccatttat cgagaggttg tgaaatcagc gaattaacct gagtattaga
     4201 gaaaaaaatg gaaaaatagt ccctcatatt ttaccgtttg ttgaactaat accgagggtc
     4261 agctgattga acaccgttta gtggagaggt cgtaagccta ccccccagct cctagttaca
     4321 aggacaaatt caaaaaacag ctcttcatat tttatgaatg ttgtacttgt gattcggagg
     4381 tggcaaggga acactgttta ttcaaaaaga ggctgggaca gaagcgttta actccaagaa
     4441 ataagaagaa attcacgaaa attgcttttc aaatttttgt gaatttcttc ttatttccga
     4501 aggagttgct tctacttccg ccgtttatac gtgtttagag cgtgagacaa aagtgttctt
     4561 tacttttgtc ccacgctctt taatgttttt aagattcaac tgaatagttt ggtgcttctt
     4621 ttgtgatttg tacatcatga gggtgtgatt cttttaatcc actgccgctc atttggataa
     4681 attgcgcttc atcacgtaat tgctgtagat tggctgcccc aacataaccc attcctgaac
     4741 gcaaaccgcc gatcatttgg aaaataatat ctgcaacact tcctttataa gcaacccgac
     4801 cttcgattcc ttctggtact aatttatttg cttcattgac actaccttgg aagtaacggt
     4861 cttttgaacc tttttccatc gcgcctaatg agcccatacc tcggtatgtt ttgaatcgac
     4921 gtccttggaa gatttcgaat tcacctggtg attcatctgt ccctgccaac atacttccta
     4981 gcatcactgc atgtccgccg gcagctaatg ctttgacgat gtcaccagaa tatttgatcc
     5041 ctccgtctgc aataatcgct ttgccatatt gacgagcaac tgatgctgca tcataaatag
     5101 ctgtgagctg aggtacacct acaccggcta ccacacgtgt cgtacaaatc gatcctggtc
     5161 cgataccaac ttttacgaca tctacacctg catcatataa agcttttgtc gcttcagccg
     5221 tcgcaacgtt tcctgcaatc aatgttgcat ctgcaaaagt tgaacggatt tcttgaattt
     5281 ttcggatcac acctgcacta tgtccatgag ctgtatcgat gatgattgca tctgcccctg
     5341 cttctaataa agcattcgca cgctcaaatg tatcgcttgt tacaccaacg gctgctgcta
     5401 caagcaatcg accatgttca tcttttgctg catttggaaa ttcgatcact ttctcgatgt
     5461 ctttgatcgt gatcaatccg cttaaacgac cttcgttatc tacgattggt aatttttcga
     5521 ttttatgttt ttgcaaaatc ttttctgcat cttttagaga ggtacctact ggggcagtca
     5581 ctaagttgtc ttttgtcatc acatcagcaa tcggcatgtg ataatctgtt acaaaacgca
     5641 tgtcgcggtt tgtaattatc cctaccaatt ttctgttttc aagtgtttca acgattggca
     5701 caccactgat acgatatttg ctcatcaatt cttctgcgtc tgctacaaga tgctgtggcg
     5761 ttaagaagaa cggatcaatg attacgccac tttcagaacg tttgactttt cttacttcat
     5821 ccgcttgctg gctgatcgtc atgtttttat ggatcacacc tagtccgcct tgtcttgcca
     5881 tagcaatggc catcttgctg tcggtaacag tatccatact tgcactgatg attggaatat
     5941 tcaacttaat atttttagct aattgtacgc tcatatccac ttcgtttggt aacacgtgac
     6001 tttctgctgg tatcaataag acatcatcaa aagtaaatcc ttttttcgtg aactttgttt
     6061 cccagttgga catttttagt accactcctc taatttagtt tatctattaa aataattgat
     6121 tttttcattt aagtcaatga tctacttgga atcaagttag ctttctcata gaataccaat
     6181 ataaagcgtt tacatctgtg tatattaaaa gagattgtaa aaaagctgtc ctgcatcaag
     6241 taataagaac agccaatcaa aaacagcttc tcatgttttt gaattggttg ggtttattac
     6301 cgcagatgca aacttttgaa caccgtttag tggagaggct gtgaaaaagc tgcttagttt
     6361 cgagtagtaa gaaaaatttt gtgaaaatgg ctttccacat ttttgcataa attggactta
     6421 ataccgagaa actggctttt gaacaccgtt tatcgtaaaa agggactgtg acaagacttt
     6481 tgtcacagtc ccttactatt atttaactat ggataaacaa aatgaggttc tttactaccc
     6541 ctagatatct agcaaaatag ctgatctttt tgctagttta tcttcttggg acggataaac
     6601 ttccaataat cccgtatttt ttcttcaagt agtaacggat tgctaaagca gttgccccga
     6661 taatgataac tactactgga tctaatactg gattgataac tggtggcaat agtgttgtcg
     6721 cagaaaacag tacgacccaa agaagtgtcg ttccacctag gataagcaat gtcttaccca
     6781 ttcctggttt cttgcttcga tctgctccgg gatattcata ttgatagatg tatttgtaca
     6841 tcatataaaa gacccagcca ccgattgcag aacctagaac gagtgtagaa aagccataag
     6901 ctggcgttgt tccacgtgaa aacaatccgg taatcgcaat catgatactt aatagaccaa
     6961 gcaacagcaa cgtattatct aaccacatca ataaaggggt ggatgaattt tttacttctg
     7021 ctggcttatt gatgattgct tccgttcttt cagagactgt cccaaacaat tgacgggctg
     7081 ttcttccgcc tttttgttct gcaactaaag taggcagaat gtcatgaagg gcttttactt
     7141 tttcttcttc agacaaattc gctgcttcta aagatttttt caaatcaaaa atgtactgtt
     7201 gatttctttt ggttaatttt gtttctaaac tattgttttc ttttacgatt gctcgtaatt
     7261 gttctggttc cattatctaa tagtcctcct tagccgttcc ttttctcaac ggtcgtcaca
     7321 aaagacattc taccatattc tacagctagt ttgatagccc ttttacacat taaagcggaa
     7381 taacattacg tcgccgtctt gtacgacata atcttttcct tctaatcgaa cacgtccagc
     7441 ttcttttgct gcatgcatgt ttccgtaatg atccaagtct tcaaaagaga cagtttctgc
     7501 acggataaat ccgcgttcga aatctgaatg gatgattcct gctgcttgtg gtgctttgat
     7561 tccttttttg aatgtccaag cacgtacttc ttgttcccca gcagtaaagt aagtagctaa
     7621 gcctaacaaa tcataagcag cacggatcaa ctgatccaaa cctgattctt cgattcctaa
     7681 agcttctaaa aattccgctt tgtcttcttc atctaattct gcgatttctt cttctgcacg
     7741 agcactgacc acgatcactt ctgcattttc ttctgcagca aagttgcgca cttcttgtac
     7801 ataatgattc gcgtctgcat ctgctacgtc atcttctgag acattggcta cataaagaat
     7861 tggttttgtc gtcaataaaa atagtgattt aacgattttt tgttcttctt cagtaaattc
     7921 gatcgttcgg gcagattttc cttcttccag tacaggtttg attttatcca gtacagctag
     7981 ttctgccact gcatctttat ctttggtttt agcaactttt gctacacgag tatagcgttt
     8041 gttgatagat tctaagtctg ctaacactag ttccaagttg atcgtatcaa tatcttctag
     8101 tggatcaaca cgtccttcta cgtgagtaat attatcatca tcaaaacagc gaacaacgtg
     8161 acaaatagcg tctacttggc ggatatggct taagaattga tttcccaaac cttctccttt
     8221 acttgcgcct tttacgattc cagcaatatc tgtaaattca aaagtcgtcg gaacagtttt
     8281 tttcggatgt acaagttctg ttaagcgttg caaacgccag tcaggtactt ctaccatccc
     8341 tacgttgggg tcgatcgtcg caaagggata gtttgctgct tctgctcctg cttttgtgat
     8401 cgcattaaac aaagttgatt ttcctacgtt gggcaaccca acaattccag cagtcaatgc
     8461 cataaattta ttcactcttt ctctgcattt ttttcaatta ctttttttaa tttcttttca
     8521 aaatctcgac gggtcatcat gacgatatgt ccgcaattca tacatctgat tttgatatct
     8581 gcgcccattc gaatgacttc ccagcgattc gtctggcaag cgtgaggttt tttcatttca
     8641 acgatatcgt gcaagtcgta cataaaaata gcctcctttt tgtttattct tcatcaaatt
     8701 gaatattcaa gatatcaaga attcgagtca aatctttttg agaaagatat tcaatctcga
     8761 tcttaccttt cccatttttt tcttggatag ctacacttgt accaaattta tccatcaaac
     8821 gatcttcgct ttctcggata taatatggtt tttcctttgc tagccttggg atttttttct
     8881 tttcttttcc atcattttga tttaactcag aaacaagctg ttccagttgg cggacagtta
     8941 aattttcagt tactgcacga ttggctaatt tcaagatcaa tgttttattt tttaaaccta
     9001 acagcgtacg ggcttgtccc atagataaac gctgatcttg taccatttcc ttgacaagat
     9061 ccggtaaaga aagcaagcgc aaataattgg cgatataagg tctgctcttt cctaaacgac
     9121 tagctacttc tgcttgtgtt agtttcaggt ttttcatcag catctcatat gcttctgctt
     9181 cttctaacgg attcagatct tctcgctgca agttttccag cactgctact tgcatcatcg
     9241 cttcttcgtc aaagtctcta acgatagcag ggatcgtttc tttttcagct agtttggacg
     9301 ctcgaaaacg tcgctcccct gcaataatct catatccttt tactgcggaa cggcgtacga
     9361 tgatgggttg aaagacacct gattgttcaa tagaattagc tagttcctgc aaagatactt
     9421 cttcaaatgt tttacggggc tgatacggat ttggtcgcag ctcattcaat ggtagctgga
     9481 caacggtatc atcttttaca tccaccgtct ctaagttttc taaatcttga aacagtgcat
     9541 cgattcctcg gcctaatcct ttacttttct tcacgactta ccacttcctt tgccaatgct
     9601 tggtagactt cagctcctcg agaacgtgga tcgtagtcga tgatcggttt tccatgacta
     9661 ggtgcttctg ataaacgaat atttcttgga atgatcgttt cgtaaacttt ttcgcggaag
     9721 tatttacgta cttcttctac gacttcattt cccagatttg ttcgtgcatc atacattgtc
     9781 agtaagactc cctcgatctc taattctgga ttaaagtgtt tttggactaa acgaaccgta
     9841 ttcagtaatt ggcttaatcc ttccaatgcg taatactcac actgaacagg tatcaaaatc
     9901 gaatcacttg ctgtaaatga attgattgtc agatggccaa gagaaggcgg gcaatcgatc
     9961 aaaatataat catactgact gcttacttct gctaaagatc cttttaatct tgattctctt
    10021 gccatcattg acgtcaattc gatctctgct cctgctaatt ggagagtcgc aggcacaata
    10081 ctcaaatttt cgtgttcagt gatcaaagtt gcctcatcaa ttggcaattc attgactaga
    10141 acatcataga tgtctcttgt cacatcaggt tttctgatcc cgacaccact ggtagcattt
    10201 ccttgtgcat ccatatccac tagcaatact tttttgccaa ggctagcaag acaagcacct
    10261 agattcaccg tcgtcgttgt tttgccgacg ccaccttttt ggttcgcaac agatattatt
    10321 tgtgccatct cttctcctcc tatttgattg gctgcttatt aggcagtccg ggctttcttg
    10381 ggtatttctt tggggttggt ttcttttttt gaataacgat gatatgccgt tgatcagaga
    10441 cattcggcaa ggtaaccgcc tcttctttga ggaatttacc acctaataaa gcaatcgctt
    10501 gttttgcttc tgtcaattcc tcttcacttt ttgctgcttt caatgcataa aaatagcctt
    10561 cttgtttagc caagggcaaa cacaattccg ctaacacatt caaacgagca accgcacgag
    10621 cagtgacaag gtcaaatgat gcacgaaagt gaggattttg cccaaatgtt tctgcccggt
    10681 catgatataa ggaagcatcg attcctaata atcgggttaa ctcagataag aaggttattc
    10741 gtttattcag agaatcaacg atcgttatct gcagttctgg aaaggcaatt tttaaaggta
    10801 tgctaggaaa ccctgccccc gaaccaacat cgcataaaga taaaggttgc gtaaagtcta
    10861 cttcaaatgc taatgtaagt gaatcgtaaa aatgcttcag atatacgtct ttttcatccg
    10921 taatagctgt aaggttcatc ttttcgttcc attcgactaa tgtccgataa taaatagcga
    10981 attgattcat ttgatgatcg gtaagctcta tccctttttc tgccaaggat tggcgaaatt
    11041 cttctggtat cataaatatt cctttcattg tgaaacagca attgtttcac agattctctc
    11101 cctactttaa cgcaaaatct ctagacatgc aatccttcca tatggattct taaaaatttg
    11161 aaaagtaaga aaagccgata gactttttct ttctagtatg actcataaaa tatacacaga
    11221 agagagaact agctttgagc aatttctgca aaaactagtt ctctcttcta tttattatta
    11281 cgtattacgc tggttcactg actttcgcaa tcttcccttg ttcgatatag accattagga
    11341 tacttaggtc tgcagggttt acgccactga ttcggctagc ttgggcaatt gtttctggtt
    11401 ggattttctg caatttttgt tttgcttctg ttgctaatcc gttgattgct gcgtagtcga
    11461 tgttttctgg aatctttttc gcttccatgc gttttaattt ttctactttt tccatggctt
    11521 ttttgatgta gccttcatat ttgatttgga tttctacttg ttcgatttct tttcgtgtca
    11581 attcttcatt ttctggaata aaccgtagaa gttctgcata agtaatctca ggacgtctta
    11641 aaaagtcgct tgctagtaca ccatctttta atggtgccgc attaatcgtt gctaaaaagt
    11701 cttggacttc ttctgttggt ttgattcgta cattcatcaa acgtttgatt tcttgttcaa
    11761 ctgcttcttt tttcttcaaa taattcttgt attgatcttc ttttactaag cctacacggt
    11821 gacccatttc tgttaatcgc aaatctgcat tatcatgacg taaaagcaga cggtattcag
    11881 cacgtgaagt cagcaaacga taaggttcgt tcgttccttt tgttaccaaa tcatcgatca
    11941 tgacaccgat gtaaccgtca cttcgtttga tcactaaagg ttcttttccc tgaactttca
    12001 aagctgcatt gataccggca atcagccctt gtccagccgc ttcttcataa ccgcttgtac
    12061 catttgtttg tccagctgta taaagatttt caacgacttt agtttctaat gtcggacgga
    12121 gctggtgtgg ctctactacg tcatattcga tagcatatcc tgtacgcatc atttctactt
    12181 tttctaatcc agcaatagaa tgaagcatgt tgacttgaac atcctctggt aaagaagtag
    12241 ataaaccttg aacatagact tcttccgtat ttaagccttc tggttccaaa aataattgat
    12301 gacgtggttt gtctgcaaaa cgcacgatct tatcttcgat ggatggacaa tagcgagcgc
    12361 ctaccccttc aacgatccct gtaaacattg gggcacgatg taagttgtct cgaataattt
    12421 ggtgagtcgt ctcacctgta taggtcagcc agcaagattc ctgatctagt ttataggcac
    12481 tatctggtgt gctgaagcta aaatgattcg gctctttgtc accaggctgc tcttctgtaa
    12541 cagaatagtc gatcgtactt gattttactc gaggcggtgt tcctgttttg aaccgttcga
    12601 tagcaaagcc aagttctttt agatgattcg ctagtccaat agaaggttga gagttattcg
    12661 gacctgatga atatttcaat tcaccgataa tgatttctcc gcgaagtgct gttccggctg
    12721 taataacaac tgctttggcc cggtagctcg ctcctgtgga cgtcactact ccatgacaaa
    12781 caccatcttc gacgatcagt tcttcgacga ttccttgtct tagcgtcaaa tttggttctt
    12841 tttcgatcgt atgtttcatt tctgttgaat aagcatgttt gtctgcttgt gctcgcaatg
    12901 ctcggacagc tgggcctttt cctgtgttca acatacgcat ttggatatag gttttatcga
    12961 tattcttacc catttctccg cctaacgcat cgatttctcg aacgacaacg cctttagctg
    13021 gtccgcctac agaaggatta catggcatga atgctaccat gtccaaattt attgtcagta
    13081 ataatgtttt tgatcccata cgtgcagcag caagtgcagc ttctgaaccg gcgtgtcctg
    13141 cgccaacaac gatcacgtca tacgcatctg cttcataatg ttccaatcgt ttgttcctcc
    13201 ttttattttc ctaaacaaaa ttgactgaat aattgagtga ttagctcatc ttgtacactg
    13261 tcgccgacaa cttctcctag atagtcccag caacgcgtca tatctatttg aacgagatcc
    13321 acaggcattc ctgaatcaat acctgcaatg acttcttcaa gtgagaggga agctttttct
    13381 aacaacgcga tatggcgggt attagaaaca taagtagcgt ctctttctcc tgtttctccg
    13441 ctgaagaata aatcagaaat agctgattcc aaacgatcta gtccgtcgtt tttagctacg
    13501 gaaattgaaa aaactggttc attttcgatc aatttcttca atttttcttg ttccagctgt
    13561 gctggcaaat ctgttttatt tagaagaatg atccgtttca atccacttgt cgcttccaat
    13621 aattgttcat cttctgctgt cagtggttcg ctttggttta gaacaagtaa gatcaagtct
    13681 gcttctgcta atgcttttcg gcttctttcg accccgatcc gttccaccac atcttctgtt
    13741 tcccgaattc cagctgtatc gattagcttg agcggtaccc cccgaacatt gacatactct
    13801 tcgattacat ctcgagtcgt tcctgcaata tccgtaacga ttgctttttc ttctcttaac
    13861 aaatggttta acaaacttga tttgccgaca tttggccggc cgatgattgc cgtacttaac
    13921 ccttcccgca gaaccttccc ttgcttggat gtagcaagta gcgcttggat tctttgttgg
    13981 atcatctgcg ctttttcaag caataacttc gtagtcaatt cttcgacatc atcatattct
    14041 ggataatcaa tatttacttc tacttgtgct aatgtctcta aaatctcttg tctcaatgag
    14101 cggatcaaag cagacagatt gccgtctaac tgattaagag ctagtcccat tgctttgtct
    14161 gtttttgccc gaatcaaatc catcacagct tctgcctgtg aaaggtcaac acgaccattc
    14221 aagaatgctc gttttgtgaa ttcgcctggc tcagctaatc ttgcaccttc tcttaacaat
    14281 agctgcaaaa tttgattgac tacaacgatc ccaccgtgac aattgatttc tactacatct
    14341 tcccgcgtaa atgtctttgg tgctctcatc acggagacca tgacttcatc gacaagctga
    14401 ttgctcttgg gatcaacgat atgtccataa tggatcgtat gacttggtac ttctgataat
    14461 cgtttgtttc cagattgata aactttatct gctagttgca cagcttgatc gccacttaaa
    14521 cgaacgatac tgattgcacc ttctccaggt ggggtagaaa ttgccgcaat cgtatcaaat
    14581 tctaaagtaa tttcagccat tttttcactt cctttttcat tttgcgcaca aaaaaagtgc
    14641 ccactccacc ctttatgcac aataaacggt gaacttcgca ctttgactgc ttgttcttga
    14701 ttaaattctg gtcatagatt atcacacttg ctataaggaa tcaagtgttt aaaacaatta
    14761 gatgaagttg agcctgttct gcttccatta ccttatattt aattgtcctt ggatgtctct
    14821 ttgcgttctt tggtttattc gaagagaaga tactgtataa acaaggcttt atctttctta
    14881 aaaaaaacgg tacactaaat ggtagcgcta tctaaactat acacttttga aaggagaaaa
    14941 tatgtgagcc cgttcagatc tgctgcattg gaagaagaaa gtgaccctcg gatcaacgat
    15001 gtcgttttat ctacagggaa tatcaaccag aaatatatcg tccgcgatat cgtttttgtt
    15061 gtggaatgct tagaagctga tttgtttgac ccatcttttc ccaaagatga ccttttctta
    15121 acaacgaaga ggaaattaaa gacgaaggta cttgaatatg gtgcgaatgc agtaatcaac
    15181 tgtcattttg agtatcagca aaaaaaacgc gacggaaagt attttctgga actttttgct
    15241 tatggaacag tgatccaatt tatccaaaca acgattggtt aaatgaaaca gacaaaaaaa
    15301 gcggatcaaa actgaagaca gtttcgatcc gcttttttct atcaaagtta gtgactactt
    15361 catcgtatac tctgataaga atgatttcta gtaaaatata cttggtttga aaaactttgt
    15421 caacgtgatt tattcaaaaa gtaatggaat caataatatt ttttagcagg ttctactact
    15481 aaataacgat aaggttcctc accttccgaa tgtgttttta catgttcatc tttgcttaat
    15541 gcagcatgga tttgtttacg ttcaaatgca ggcattggtt ctaagaaaac aggctgccct
    15601 gtccgtttta ctttttcagc cgtgcgatca gctaaacgct gaatgatctc ttggcgcttt
    15661 tcacggtaat tgcctacatt gactactaca gacagcttat ttcttgctaa acgatggatg
    15721 aagacttgtg ccagatattg caatgcattc agtgtttttc catgtttccc aatcagtatg
    15781 ccttgctttt gtgtttctaa atggaaaaca accatattat ttgattctct ttccattttg
    15841 acaagagctg gagcatcaag ctgttttgaa atttcagtaa gataaactgc caattccgtt
    15901 aaagctgctt catcacctag ctcacttact tcttctgttt tttcttcaag aatcgtttcg
    15961 ttgactacct cgatctcagc tgttggttct tcttccaaaa tttcttctac agtctgttct
    16021 actgtttcag aaactttttc gctgatactt ggctggatcg aaagccgtgc attctttcga
    16081 cccattccta ggaaaccttt ttttccttct tccagtattt cgatttcgac ttggtctttg
    16141 gccaaaccca aatcagccag acctagttgg acagcttcgt ctattgttgc agcttcataa
    16201 atcggcatta gcattacctc ccctattatt tccgtttttt cttaggattc attgctttct
    16261 taagagcacg ttctctttcg cggattctcc gtgcttcttc ttcacgctct tttcgaattt
    16321 tgaaagggtt gttgatcagc agcgtttgta caacttggaa ggcattggaa actacccagt
    16381 ataatgaaag accgctggct aaattgacac ccatcactaa aatcattaca ggcatcacga
    16441 aattcatgat cttcattgat gcattcgatt caatttgact catgctagat aaatacgtac
    16501 ttgcaaaagt aaagattgct gctaaaatcg gcaaaataaa tgttggatcc gcgttcccta
    16561 agttcaacca taagaaatgc ccttgtttca actctggtac acgtgaaata gattgccata
    16621 acgccatcaa gattggcatt tgtactaata aaggaagaca accagcatat ggattgacgc
    16681 cattttctgc atataaacgc tgctgctctt ctcttaactt actttgtgtt tccggatctc
    16741 ttgaactata ttgttgctgc agtgctttta atttcggttg taattcttgc gttttacgca
    16801 tgctctttgt ttggaaatgc atcaacggca ataggatcac gcggatgatc agtgtaaata
    16861 aaatgatccc tatccctgca ttaccaaatg acaacgcctt gatcgcctca gcaaagtaat
    16921 aaacaatata acgatcccaa attcctgtac tctgtgcgct gacttcccca gttccgcaac
    16981 cggataagac gatgaccaaa ctaacgatac cggccatcaa aagtaaacgt ttgtatttct
    17041 tcacttactc ttttcctctc tgtcgattat ttttgcaagt tttaagacgt ggactaaatt
    17101 ggagcgaacc tcatcataag tcaacccctt cacacttggt ctagcaatca caataaaatc
    17161 caactccgga tcaatttctt cttttataga ataaactgct gctcggattt ttcgtttgac
    17221 ttcattgcgg tttaccgcgt tgccgacctt ttttcctaca gataaaccaa ctcgaaaatg
    17281 aggctgtccc ggtttttcca aacggtaaac gacgaatttg cggttagcaa aagaatctcc
    17341 cgtatggaac acggcttgaa actctcgttc tttttttact cgatacgctt ttctcattag
    17401 ttttctttcc ttttctatct ctcacttacg ctcataatca taccataatt acgattgtcc
    17461 tttcatcatt tccctaaaaa agagtataaa aaaaaccact gaaaccccag tggcctaagc
    17521 tgaaagaact tttcttcctt tgcgacgacg gctagctaac acacgacgtc catttttagt
    17581 actcatacgt ttacggaatc cgtgaacttt ttgacgttta cgtttatttg gttggtatgt
    17641 tcttttcata tattccacct ccaaaatgtt cttatctatt ttataaacac agacatactt
    17701 tgatagtata acgacaaagt agtgattttg tcaatataaa atagaagatc gattgttaaa
    17761 acttatccac aagattattt ttttctatgg gaatcctatg tggaaaaagt tatgcacagg
    17821 gattatccac agataataaa aggctggttt tacacagaaa acacacgtgt aaaaaaagta
    17881 atccacaatc aggcgatttc ctgtgtattc cgtctcctaa ctattggtat atctgtattt
    17941 tttatccaca gacgcaagag gaaatctatt ggtttttacg agttttccac ctgtgcatta
    18001 gtggtgtgga aaactttttt tgacaactgt gtttttctct tttttctttt tgtcgattgt
    18061 gtggataatt gtaattaaaa aatttttttc ttcggtttct gtggaaaact attgaaaaaa
    18121 atgctaaatt agtactagtt attcactttt taatgaagga ggaggcctta tatggtatcc
    18181 ctcgatgctt tatggaatga attaaaagca acataccaaa aggatttatc gcctgctagc
    18241 tataatacat ggatcgaaac agcgaaccct cgaacccttg atcaaaacca gctcgttgtt
    18301 gaggttccta gcaagattca taaagaatat tgggaaaaaa acctggcgac aaaaatcgtt
    18361 gaagtcggtt atatgttatc aggaaatgag attatccctc gctttattac tggcgaagaa
    18421 gcagagcaag aagaagttat agaagaaaaa aatccaaaag ttgtggcacc tagtccgcta
    18481 aaaaaagcca tgctgaaccc taaatatacc tttgatacgt ttgtcattgg taaaggaaac
    18541 cagatggctc atgcagcagc gctcgttgta gcagaagatc caggttctat ttataacccg
    18601 ctcttctttt atggaggcgt gggacttggt aagactcact tgatgcatgc aattggtcat
    18661 caaatgctgc aaagtcagcc taatgccaaa gtaaaatatg taagcagtga aacttttgct
    18721 aatgatttca tcaattcgat ccaaaacaaa acagcagaag agttccgtca agaatatcga
    18781 aatgttgatt tactgctagt ggatgacatt caattttttg cagaaaaaga agctacgcaa
    18841 gaagagtttt tccatacttt caatgccttg tacaatgaag ggaaacaaat cgtactaacc
    18901 agtgatcggt tgccaaacga gattccgaaa ctgcaagaac gccttgtttc tcgctttgca
    18961 tgggggctgt ctgttgatat tacaccgccg gatcttgaaa caagaacagc gattttacgt
    19021 aaaaaagcgg cggctgaacg tctagagatc ccagatgaca cactaagcta catcgcagga
    19081 caaattgatt ctaacattcg tgaattagaa ggtgcgcttg ttcgagtgca agcttttgct
    19141 gcgatgaata gtgaagatat ttcgactagt ctagctgcag acgcactgaa aacgttaaaa
    19201 tcaggaaaag gacatcctca attgtcaatc ctgcagatcc aagaagaagt ggcaaagtat
    19261 tatcacatcc aattgaaaga tctcaaagga aagaaacgcg tcaaatctat tgttgttcct
    19321 agacaaatcg ctatgtattt ggcacgtgag ctcacagata attctttacc gaaaatcgga
    19381 gctgaattcg gtggcaagga tcatacaacg gttatccatg ctcatgaaaa aatccagcag
    19441 ctgatggatt ctagtgtcag tatgcaaaat gaagtatcag aaatcaaaaa ctttttacta
    19501 aattaatttg tcgattctat caactgaaaa tagcatgtgg ataaaagaaa aaaacgttca
    19561 aaagttatac acactttttc cacagctgtt ttttcgtttc tctcttacgt tttttgagtt
    19621 ttccacagaa tcaacatgcc ctattacttt tattattctt tttaactaat aataaataaa
    19681 caaacattca aaggagatac atcatgaaag ttactttaaa ccgagctagc tttatgcagg
    19741 aattgcaaac tgttcaacga gctatttcaa gcaaaaccac gatccctatt ttgacaggtg
    19801 taaaaatcac actgacacaa gaaggtttga ctttgacggg gagcaacgct gatatatcaa
    19861 ttgaaacttt tttgtctgtt gaaaacgaaa aagcaaatat gcaaatcgaa tctactggtt
    19921 ccattgtttt acaagcacgt ttctttagcg aaatcattcg gagacttcct gaagaaacat
    19981 ttactttaga agttttagaa aataaacaag tagcgatcac ttctggaaaa gcgaatttta
    20041 tcgtaaatgg attagatgca gataactatc ctcatcttcc tgttgtcgaa agccataacc
    20101 agatgaaatt acctgtacac gtattgacta aactaatcaa cgaaacagtt tttgctgtct
    20161 cacaacatga gagtcgtcca atcttgacag gtgtgcattt cattttatct gataattctt
    20221 tattagctgt agctactgat tctcaccgtc taagtcaacg cgtgattcca gtagaacaag
    20281 cggctgatca ttttgatatt gttattcctg gaaaaagttt gatcgaatta tctcgctcat
    20341 taacaaatga agaagaaatc gtcgaaatca gcattatgga aaaccaagtg ttgttcaaaa
    20401 cagaaacgat gtatttctat tcccgtttgc tagaaggaaa ctatccagat accaatcgtt
    20461 tgattccttc aagttttaat acagaggttg aattttctgt tcctagcttt ttagcggcga
    20521 tcgaacgggc ttctttactt tctcatgaag gccgtaacaa catcgttcgt ttatctattc
    20581 gtccagatgc ggttgtttta tacggaaatt cgccagaaat cgggaaagta gaagaaagct
    20641 tgagttatac agcaagcagt ggcgatccat tagatatttc tttcaatccg gattatatga
    20701 aagcagcgtt gcgtgcattt ggagatatga gcatcaaagt gaaatttatc tctgctattc
    20761 gtccatttac attggaacca acggaagatg gcgttcaatt tattcagctg attactcctg
    20821 tacgtacaaa ttaacaatga ttttcaatgt tcttgaaaat ttaattgaaa agaaatagaa
    20881 aaaataaaat tttaaacaga aggccctaaa atctctttta aggctttctg tttatttttg
    20941 tttaaatcac cttttttaga ttaaatcgtc ttatatggaa aaatatatgg ataaatttgt
    21001 tttattgcga aaaaaaaggt ataatagagt atacttttaa taatgaaaat gagggatggg
    21061 cattgaaaca acaagtcatt ctaaatacgg aattcatgac gttgggccaa atgttgaaag
    21121 aagtcacagt tatcggtagt ggcggacaag caaaatggta tttggcagaa aacactgtgt
    21181 tagtcgatgg cgaaccagaa aatcgtcgtg gacgcaaact ttatcccggc atgatggttg
    21241 aagttccaga agtaggaact ttttttatgg taaaaaaagg aaacgacgat aatgaggctg
    21301 aataagctgt atttaaaaaa ttatcgaaat tacgaagagc tgaacatcga attttccaag
    21361 aacctcgtca tctttttagg agaaaatgcg caaggaaaaa cgaatctttt ggaaagtatc
    21421 tacgtattag ctatgacgcg aagtcatcgg acaagtagtg agcaggaatt gattggctgg
    21481 actgacgatc aggcgatgat tcaaggagag atcacaaaag gttcatccac tcttccatta
    21541 gaaatcttat tatctaaaaa aggccgaaaa accaagatca accatatcga acagaaaaag
    21601 ttaagtactt acgtgggaca aatgaatgtc attttatttg caccagaaga tctttcttta
    21661 gtaaaaggca gcccgcagac caggcggaag tttttagata tggaattagg ccagatcaat
    21721 ccaatctatc tttatgatct tgtccaatac caagcaattt tgaaacaaag aaaccagtac
    21781 ttgaagcaat tgaatgaaaa aaaacaaact gatttgcttt atttggatat tttgtccgaa
    21841 caactagcag cgtttggcag caaagtattg aaagcaagag cacagttcgt caagcgattg
    21901 gagttttggg ctaattcttt gcatcagcaa atcacccatc aaaaagagca gctggaaatc
    21961 gagtatctca ccgcagtcga ttctttagaa acacatacgc aagagcaaat ccaagaacaa
    22021 tttctcgcac ttttgaacca aaataaaaag aaagaccttt ttcggggaac aacgacagtt
    22081 ggtcctcatc gtgacgatct ttcctttttt attaatcaga aaaatgtgca gacttatgga
    22141 tcgcaaggac agcaaagaac aactgcacta agtgtcaaat tagcagaaat cgatttgatt
    22201 aaagaagaga caggggaata tccaatcctt ttgttagatg atgtgatgag cgagctggat
    22261 gataatcgtc aactccatct gctagaaaca atcgaaggaa aagttcaaac gttcttaaca
    22321 acaacgacac ttgaccatgt aaaaaataaa atgactgttg aacctgagat tttttatgtt
    22381 caacaaggga aactaaaagg agaagaaact gaatgacaga agaaagaagt ttagtagaac
    22441 gcgctaaaga gtatgatgcc agtcagattc aagtacttga aggacttgaa gccgttcgta
    22501 aacgacctgg catgtatatc ggttcgacta gttctgaggg tctgcatcac cttgtttggg
    22561 aaattgtcga taattcgatc gatgaagtat tagcaggttt tgcaacgaaa atccatgtga
    22621 ttattgaaaa agacaacagt atcaccgtta ttgacgacgg tcgggggata cctgtagata
    22681 tccaggcaaa aactggacgt cctgcagttg aaacggtctt tactgtttta catgctggag
    22741 gaaaattcgg cggtggtggg tacaaagttt ctggtggact tcacggagta ggatcttctg
    22801 ttgttaacgc gctttctaca cagcttgatg tcaaagtata caaagaagga aatgtgtact
    22861 atcaagaata tcgtcgtgga gctgtagtgg acgacttaaa gattatcgaa caaacggatc
    22921 gccatggaac aacggttcac tttacgccag accctgaaat ttttacagag acaactgagt
    22981 ttgatttttc aaaattggct acccggatcc gtgaacttgc tttcttgaac cgcgggatgc
    23041 gtatatcgat tgaagacaaa cgagaagaag aaccagtcat caatgaatac cattatgacg
    23101 gcgggattaa aagttatgtc gaatacttga atgcaaacaa aaccgtgatt tttccagaac
    23161 ctgtatattt ggaaggtgaa cagcaagata tcgcagttga agtctctatg caatatacag
    23221 acggctacca ttccaatatc atgagttttg ccaacaatat ccatacgtat gaaggcggaa
    23281 cacatgaatc aggattcaag actgctttaa ctcgtgtgat aaatgattat gcccgaaaac
    23341 aaaagctgat gaaagaaaat gacgacaact tgaccggtga agacgtgcga gaaggattga
    23401 cagctgttat ttctatcaag catcctgacc ctcaattcga aggacaaaca aaaacaaaat
    23461 tgggcaattc cgaagtacgg acagtcactg atcggttatt tagcgaacat ttcatgaaat
    23521 tcttgctgga aaacccaagt gtgggccgtc aaattgttga aaaaggatta ctagcttcca
    23581 gagctcgtct agcagcgaaa cgtgcgcgtg aagtaacgag acgtaaaggt gctttagaga
    23641 ttagtaatct gccaggaaaa ttagcggact gctctagcaa cgatcctgaa aaatgcgaat
    23701 tgttcatcgt cgaaggagat tcggccggcg gatcagctaa gcaaggtcgt aatcgagagt
    23761 ttcaagccat cttgcctatt cgtgggaaaa tcttgaatgt tgaaaaagca agcatggaca
    23821 agatcttggc aaatgaagag atccggtctt tatttactgc tatgggtaca gggtttggag
    23881 aagattttga tgtatcgaaa gctcgctacc acaaattagt tatcatgact gatgccgatg
    23941 tcgacggcgc gcatatccgt acattgctgc tgactctttt ttatcgttac atgcgtccta
    24001 ttgttgaagc tggatacgtc tatattgctc agccaccttt atacggggta aaacaaggga
    24061 agaatattac gtatgtacaa ccaggaaaaa atgcagaaga agagttgaaa caagtcgttg
    24121 cttcacttcc tgcctcacca aaaccaagcg ttcagcgtta taaaggactt ggagaaatgg
    24181 atgatcatca actatgggaa acaactatgg acccaagcaa tcggatgatg gcccgcgtca
    24241 gtgtggacga tgctattgca gcagatcaga tatttgaaat gttgatgggg gatcgagtag
    24301 aaccccgtcg tgcattcatc gaggaaaatg cacactatgt gaagaactta gatatttaat
    24361 catgaaggag gaaaaagaat gagtgaagaa atcagagaaa acatccatga tgtcaatcta
    24421 accagcgaaa tgaaagactc cttcatcgat tatgcgatga gtgttattgt cgcacgtgca
    24481 cttcctgatg tcagagatgg tctgaaaccc gttcatcgcc gtattttata cggcatgaac
    24541 gaattaggtg taacaccgga taaagcccat aaaaaatctg ccagaatcgt cggagatgtc
    24601 atgggtaaat accatcctca tggagactat gcgatctatg agtcaatggt acggatggca
    24661 cagccattca gctaccgtta tatgctagtg gatggtcatg gaaactttgg ttctgttgac
    24721 ggtgacggtg ccgccgctat gcgttatacc gaagcacgga tgagcaaaat cgctacagaa
    24781 atgctgcgtg atatcaataa gaacactgtt gatttccaaa gcaactatga tgatacagaa
    24841 aaagaaccag ttgtactacc tgctcgcttc ccgaatcttt tagtgaacgg aacaacgggg
    24901 atcgccgtag gtatggccac gaatatccca cctcataact tgtctgaggt agtagcggct
    24961 atcgatttgc tgatggataa tcctgatgtg acgaccaatg aattgatgga agtattacct
    25021 ggaccagatt ttccaacagg tggtttggtt atgggaaaat caggaatccg aagagcgtat
    25081 gaaacaggta agggttctat taccgtacgt gcaaaagttg agattactga gatgccaaat
    25141 ggtaaagaac ggatcatcgt gacagaattg ccttatatgg tcaataaagc aaaattgatc
    25201 gaacggattt ctgaactgca cagagacaag cgtatcgaag ggatcacaga tttgcgggac
    25261 gaatcttccc gtgaaggtat gcggatcgtc attgacatcc gtcgtgacgc cagcgcatct
    25321 gttatcttga ataacttata taaattgaca tccttgcaaa attcatttgg attcaatatg
    25381 ttggcgatcg aaaaaggtgt gccgaaagtt ttgagcttga aacaaattct tgaaaactat
    25441 atcgaacatc agcgcgaagt catcacaaga cggacagtct ttgaaaaaga aaaagcagaa
    25501 gcaagggcac acattttaga aggtttgcgt attgctttgg atcatatcga tgaaatcatt
    25561 gcgattatcc gcgggtctaa atcagatgac gaagcaaaag cgacgatgat cgaacggttc
    25621 gatttatccg atcgccagtc tcaagctatt ttagatatgc gtttacgccg attgaccggt
    25681 ttggaacggg aaaaaatcga aaatgaatac caagaactct taaaactgat tgaagattta
    25741 actgacattc tggcaagacc agaacgtgtt acagaaatca tcaagaatga attagcagac
    25801 ttgaatcaac gatttggtga taaacgacgc acagaattac tagtcggtga ggtcttaagt
    25861 ctggaagatg aagatttaat cgaagaagaa gaaatcgtta ttacattgac aaacaatggt
    25921 tacatcaaac gtttggcaaa taatgaattc agagctcaac gaaggggtgg acgtggtgtc
    25981 caaggaatgg gtatccacaa tgacgacttc gtcaaaaatc tggtttcttg ttctacccat
    26041 gatacattgt tattctttac gaataacgga aaagtgtatc gggcaaaagg atacgaaatt
    26101 ccagaatacg ggcgcacagc caaaggaata ccaattatca atttactagg tatcgattcg
    26161 agtgaaagca tccaggcaat cattgcggtg acgggagaag cagaagaagg ccactatctc
    26221 ttctttacga cgcgtcaagg tacagtaaaa cgtacttctg tcactgcttt tgctaacatc
    26281 cgaagtaatg gtctgatcgc aattggtttg aaagaagacg atgagctcgt caatgtgcta
    26341 ctgacagatg gtcaatctaa tatcattatt ggaacacata acggctactc tgtcactttc
    26401 aaagaagaag cagtccgcga tatgggacgt acagcttccg gggttagagg aatacgcctg
    26461 cgtgaggaag attatgtcgt tggggcagcc ttaatgaagg aagatcaaga agtattagtg
    26521 atcacagaaa aaggttacgg aaaacggaca aaagtatcgg aatatccagt gaaaggccgc
    26581 ggaggtaaag ggatcaaaac ggcgaacatc actgaaaaaa atggtccact agctggattg
    26641 accactgttt ctggagatga agacatcatg ctgattacag ataaaggtgt cattatccgt
    26701 ttcaacgtat cgactgtttc tcaaactgga cgttcaacat taggcgtacg tttgatgaag
    26761 atggaagctg atacgaaagt tgtcacgatg gcagcagtcg aaccagaagc tgcagaacaa
    26821 acagttgaag aacaaggatc agaagaatag ccatcaagac taattgtgta agctagcaag
    26881 aatgaccgat taaaaaatct ggttattcct gctggctttt ttgattagtt gaatttctct
    26941 taaaatattc gaaactgttc attctgtatt gcttttgacc aaaatagagt gtataatcag
    27001 agcttgtgag tagtctaatt aattagataa ctctccttgc tcttaggaac ctaagagccg
    27061 aaagtccaca gggaggtgaa tatcaatgga aaacacgaaa tatgaaatca tgtacattat
    27121 tcgtccaaac attgatgaag aagcaaaaac tgctttaatc gaacgtttcg atgcaatctt
    27181 gaaagataac ggagcagaag ttatcgaatc aaaagattgg gaaaaacgcc gtttcgctta
    27241 tgaaatgaac ggttaccgtg aaggtatcta tcacatcgtt aaagtatctt ctccatcatc
    27301 agcaaatgct gtcaatgaat ttgaccgtct tgctaaaatc aacgacgata tcattcgtca
    27361 catgattgtt aaagaagaag cttaattgtt gtttcatgtg aaacaataga aaacgatgag
    27421 aggagatgac tattttgatt aataatgttg tattagttgg acgtctgacg aaagatccag
    27481 atttgcgtta cacagcaagt ggtacggcag tcgcaacatt tactttggct gtgaatcgta
    27541 attttacaaa ccaaagcggc aatcgcgagg ctgattttat caactgtgtg atttggcgca
    27601 aatcagcaga aactttagca aactatgctc gtaaaggaac acttttaggt gttactggac
    27661 gtatccaaac tcgttcttat gataatcagc aaggacaacg tgtatacgtg acagaagtag
    27721 tagctgacaa cttccaattg ctggaaagtc gttctgcatc tgaaaatcgc caacaaagcg
    27781 gcggattcca aagttctggt caatcagctg gcggatttgg cggaaacaat aactctaatc
    27841 aaacatctca atcatccaac ggtatgcctg atttcgaccg tgatacttct gacccattcg
    27901 gctcaagttc aacgatcgac atttcagacg atgatttacc attctaaacg aatttgatag
    27961 gagggaaata gaatggcaca acaaagaaga ggcggacgta aacgtcgtaa agtagactac
    28021 attgctgcta accatattga atatatcgat tataaagata tcgaattact aaaaagattt
    28081 atttcagaac gcggtaaaat cttgccacgt cgtgtaactg gtacaggcgc taaaaaccaa
    28141 cgtaaaatta ctgttgcaat caaacgcgct cgtatcatgg gcttgctacc tttcgttagc
    28201 gaagactaat aaatcctagg aattgaatga ctgatccttt tgatcagcta ttcaattcct
    28261 ttttttctgt aaaaataacc tctattcgtt tagtcgctcg aatgacgaaa gttatggtaa
    28321 aatcaaatta gtcatatatg ggaggaaaat tatggaaaat aaaaacaatc atttatcccc
    28381 gaaacctctc ttccttatcc tgtttatatt aatcgaagta ttggtagtct taattttacc
    28441 atttcgtttt gttagcgctt cggtcattct attgatgaat ttatggggtg ttcgctattg
    28501 gttgaaaatg aaacgttggc ttattttaaa taaacgtgaa aaaatccagc aggcttcgac
    28561 tgtagctgaa accaatctag cttatgtctc agaagtcatg cctgtaggag tgattgctta
    28621 tcagccatca agccaaaaaa tcgaatggct caatccatta gcattgaaaa tcaaagatca
    28681 aagtggatta acagaggaag aattgctaga tacttttttt actgcaaaag aaaaaaagaa
    28741 aaaccaagtc tatctagcag ataaaacatt tcagtttgat atggatacag taaagggtgt
    28801 tgtttatttt aatgatatta cccaaaacca gttgttaaaa ctccatcaga cgaacagtca
    28861 gccggtgatt ggtattttat ctattgataa ctatgcagat gccattgata aattggatga
    28921 caaagagatt tcttatttga actctttcgt cactacacta gtttctgact ggatgaatga
    28981 ataccatgtc tttttcaaaa gaatcaacgc agaacggttt ttcttcgtag cacagatgat
    29041 cgatctgaaa aagatgatgg ataaaaaatt tgatttgctg gatcgctttc gagcagaagc
    29101 tgaaaaacag agctcgccta tcacccttag tatggggatt gcttatggct caagaaccct
    29161 cgatgaaatc ggtggagaag ctcagaataa tttagatgtt gctttagtcc gtggaggcga
    29221 ccaagttgtc gtgaaagagg taaaagaagg agcaaaacct gtttattatg gggggaaatc
    29281 tgctaagaat gtcaaacgca cgcgtgttcg ttctcgagcg atgagtaccg ctttgcgtcg
    29341 gatttttgat gaaacaggcg atgtgtttat catggggcac aagtttccgg atatggatgc
    29401 tttaggctct gcttttggtg tcagttgtct ggcacgcttc aaccagaaaa aagcttacat
    29461 cgttatcaac gagaacgaga tcattccaga tgtagaacgg tgtcttgaag aaatccataa
    29521 gcatcctgaa ttagaaatgc aactaatctc tccagaacgt gccatagagc tgaaaaaaga
    29581 agaggacctc ctcgtgatgg tggattacca taaaccttct ctatcgatct ctcagggcct
    29641 atatgagcaa tttgataaga ttgtgatcat tgatcaccat cgtcgtgggg aagagtttcc
    29701 aagtaaacct ctgctgactt atatcgaatc ttcagcttct tcagcttcag agctagtttc
    29761 tgagctgatc caataccaaa gcagcaaaac aaatcgctta gatcgttttg aggccacatt
    29821 attgcttgca gggatggttg tagatacaaa tagtttcaat gtccgtacaa ctgcacgaac
    29881 ttttgacgtt gccagctatt taaggtcttg tggagcagac gcatccttag tacaatattt
    29941 attgagctcg gatttagcga catacttgga gatcagcaat ttgattgctg gaagcgacta
    30001 tgtgacttct gatattgttg tggcggcagg gacagacgaa aaagagtata atagtgttat
    30061 agcagcaaaa acagcagata cattgctttc gatgatcaat atcaatgcag ctttcgtcat
    30121 tacaaagcgg acagacggct tgattgggat cagtgccaga agtaccggca cgatcaacgt
    30181 ccaaatcatc atggaatcct taggtggcgg aggacacttc acaaacgcag ctactcaact
    30241 ggaaaatacg acagttgagg aaacaaaaca gaagctattt gctgcaatca aagaaaatat
    30301 gaatcaaata tatgataagg agtaatccaa atgaaagtaa tttttttaca agatgtaaaa
    30361 ggaaaaggta aaaaagggga catcaaagaa gttccagcag gttatgcaca aaattatcta
    30421 atcaaaaata acttggcaaa agaagcaacg aaaagcagta tcagtcagtt agcaggacaa
    30481 aagaaagcac aagaaaaaca cgaagctgaa atcttggaag aagctaaaaa gttaaaagaa
    30541 ttcttggaaa aagaagatac tgtagtcgaa ttgaaggcga aagcagggga agacagccgt
    30601 ttgtttggtt cgatcccttc aaaacaaatt gctgaagggt taaacaaaca atacaaaatc
    30661 aaattagata aacgaaaaat cgaattagaa aatccgatcc gttcactagg ctacacaaac
    30721 gtacctgtta agcttcatca tgaggtgacg gctacgatca aagttcatgt cgtacaagaa
    30781 taacaaatct tgatagcgat gttcagctgg taagtataac cagaaggaaa aaagacaaac
    30841 cgaaatttat ctgagaaaac agctgcagca atgaaagcaa aagctgcaaa tcgatttcag
    30901 attgatttcg gtttcttttt tgggaacagc aaaatagcat aaagaaaacg agtataataa
    30961 aagagaacta tttttttatt acactttcgg tacgaatgat gacttttctc tcattttctt
    31021 gtaaaatgaa gcaattgaag acaaggagcg atacgtatga atgaagtgtg gcaggatcga
    31081 atcccaccgc aaaatattga agctgaacaa gcggttttag gctctgtttt tcttgacgca
    31141 gaaacgatca ttgatgccat ggagtacata gagccaaaag acttttatcg tcgtggccat
    31201 caaattattt ttgagacaat gatcgagtta aatgatcgta atgaagcgat tgacgtggtt
    31261 actgtcaaag accggctgga acaagccaat ttgttagaag atgccggtgg tttaagttat
    31321 ctctcagact tggcattagc agttccgaca gcagcaaata tcgtctacta tgcgaaaatc
    31381 gttgaagaaa aatcattgct gcgtacattg atccagacag caacaggaat cgttacgaaa
    31441 ggattcgagc aaggagaaga agttcaaaca attttagatg atgccgaacg cagtatcttg
    31501 gaggtttcgg aaaaaagaaa tcgaagcggc tttttatcta ttgctgaagt actgaactcc
    31561 acgattgaaa atatcgatcg actagctcaa aacaatgaag aaatcacagg tctaccaact
    31621 ggctatcaag gtctggataa aatgacagca ggtttccagc ccgaagaact aatcattctt
    31681 gctgcacgtc cagcggtagg taaaacagcg tttgcattga atattgctca aaatgtagga
    31741 acaaaaacag atcgttctgt tgcgatattt agtttggaaa tgggggcaga atctcttgta
    31801 aatcggatgc tttgtgcaga aggttcgatt gaggccagcc atttacgtac aggtcagctt
    31861 tcggaagaag aatggtcgaa tctgatcgtt gcgatgggga gtctttctcg agcaaatatt
    31921 tttatcgatg atacaccagg gatcaaaatc tctgaaatcc gtgcgaaatg tcggaaacta
    31981 gcgcaagaaa aaggcaatct aggcttgata ttgatcgatt atttgcagtt gattgaaggt
    32041 aacggtaaag agaaccgtca acaagaagta tcagatatct ctcgccaact gaaaaaactg
    32101 gcgaaagagc tgaaagtacc tgtcattgct ctttcacagt tatctcgtgg agtagaacag
    32161 cgacaagaca aacgaccagt gctaagtgat attcgtgaat ccggatcaat tgaacaagat
    32221 gcggacatcg tagccttctt gtatcgagat gattattatg aaagagaagg cggagatgac
    32281 gatgatggaa acgagccaaa aaatgacaac gtcatcgaag tcatcatcga gaagaaccga
    32341 agcggtgcac gaggaacagt tgaactactg tttatcaaag aatacaataa gttttcgtcg
    32401 ctttcgccaa gagaagaatt ttaaagaaga ggttgtgagc cttgagcaat cccgagagct
    32461 ctcgtaagaa gatgcaagga gtaaccgcag gaacgactag agtacttaat ttaaacagat
    32521 ctccaaaggg attgtgacaa aagtcttgtc acagtccctt tttccgaata aacggtgttc
    32581 aatcagctga cccttggtat tagctcagca aacgtcaaaa tatgaggaac tattttgacg
    32641 tttgtttctc taatactcag gtcaaaacgc tgatcttacg acctcttttc cttaaacggt
    32701 gttctcctgc caactcctcg gtcacaagtc acaatatcat caaacatgag aagcagtttt
    32761 ttgaaattgc tccttgtgac tcagagttaa acggcaggct cacaacctct ttcttaaacg
    32821 gtgttcaaaa gcttgcatct gcggtaatac gcccaatcaa acgaaaaata tgagaagcta
    32881 tttttgtttg gctgttctta ttacttgatg caggacagct ttttcacaac ctctgattca
    32941 cggtgttcca aaagtagctt cttcgaaaaa taagtcgaaa tttcacaaaa atttgagaag
    33001 caattttcgt acattccttc ttatttcttg aagctgacca cttctgttac aacctctttt
    33061 ttaataacca ttctaaaaag ataaagttcg ttttttaagc gtttttataa aataaaaata
    33121 aaaatcattc gattttattt gaaatatgta tctattattg atacaatggt tcaggattaa
    33181 ataatataga acgaggtgtt cacatgtcat ctgtagtagt agtaggtacg caatggggcg
    33241 atgaagggaa aggaaaaatc acagactttc tcagtgaaaa tgctgaagtg attgcacggt
    33301 accaaggtgg cgacaatgca gggcacacaa ttaagtttaa cggtgtgact tataaactgc
    33361 atttgattcc ttctgggatt ttttataaag acaagatcag tgtgatcgga aacggcgttg
    33421 tagtcaatcc taaatcattg gttaaagaat tagcttattt acatgaaaaa ggtgtagaaa
    33481 caagtaattt gcgtatttct gaccgcgcgc acgtaatctt gccttatcat atcaaattgg
    33541 accaattaca agaagatgct aagggcgaaa ataagattgg tacaacgatt aaaggaatcg
    33601 gacctgccta tatggataaa gcagctcgtg taggtatccg tattgcagac ttattagata
    33661 aagaaatttt tgaagaacgt cttcgtttga atctagatga caaaaaccgt caatttgtaa
    33721 aaatgtttga ttcagaacca cttgattttg acgatatttt tgaagaatat tatgaatacg
    33781 gacaacaaat caaacaatac gtcacagata cttccgttat cttgaatgat gctttggatg
    33841 caggcaaacg cgtgttgttt gaaggcgcac aaggtgtaat gctggatatc gaccagggaa
    33901 cttatccttt tgttacttct tctaacccag tagccggtgg ggtaacaatc ggaagcggtg
    33961 tagggccttc aaaaatcaat aaagttgtcg gcgtctgcaa agcctatacg tcacgtgtcg
    34021 gtgatggacc atttccaact gaattatttg atgaaacagg agaacaaatc cgcgaagtag
    34081 gacgagaata cggtacgaca actggtcgtc cgcgtcgtgt aggctggttt gattctgtag
    34141 ttatgcgcca ttctaaacga gtttcaggta ttaccaacct atctttaaac tcgatcgatg
    34201 tattaagtgg attggaaaca gtaaaaattt gtacggctta tgaattagat ggcgaactaa
    34261 tctaccacta tccagcaagc ttgaaagagt tgaaccgctg caaaccaatc tatgaagaat
    34321 tgccaggctg gtcagaagac attacaggat gcaaaacact tgcagaactg ccagaaaacg
    34381 cacgtaacta tgtacgtcgt atctcagagc ttgttggtgt acgtatctca acattttctg
    34441 tcggacctga tagaacacaa acaaacatct tagaaagcgt ctgggcgcaa atctaaggat
    34501 aaaaacgacg actgaatcag gacgttctta ccagagcgag tcgaagtgtt tatctatacg
    34561 ataataataa cttgaacact gtttattcag aataagggac tgtgacaaaa accgtgtcac
    34621 agtccctttt tccgaacaaa cggtgttcaa aagcttgcat ctacggcact aagcccaatc
    34681 agatgaaaaa tatggagtgc tatttttatc tgattgttct tactgcttga tgcagagcag
    34741 ctttttcaca acctctccac taaacggtgt tctcctgcca actcctcggt cacaagtcac
    34801 aatattcaac aaacatgaga agctgttttt tgaaattgtt ccttgtgact cggagctaaa
    34861 cggcaggctc acaacctctt ggtaaacggt gttcaatcag ctgaccctcg gtattagctc
    34921 aaaaaacggc aaaatatgag gaactatttt ttccgttttt ttctctaata ctcaggtcaa
    34981 aacgctgatt tcacaacctc tgattcacgg tgttccagaa gtagcttcta cggaaataag
    35041 ccgaaatttc acaaaaattt gagaagcaat tttcgtaaat tccttcttat ttcttgaagc
    35101 tgaccacttc tgtcacaacc tcttattttg attagaactt gcgaaaataa accggttaaa
    35161 gtgtgtctag cgcaagagat ttgctataat agacgataag ggtaaaatgt aacgagaagg
    35221 ggttgaaaaa tgtgtatcaa attgtaacag attcttgttg tgatctgcca taccatgtat
    35281 tagcaaaaga aaaagttgat tttttatcaa tgaatctcca attgaatgat caagagttag
    35341 tagatgacct cggaaagaca ttcaattatg accgctacat gaatcagctg aaaaatggtg
    35401 cgatgcctac gacatcacaa atcaatgttg gccgatattt agaatttttc cgttcttatg
    35461 cggaaaaaca gattcctgtt ttatatgtgg ctttttccag tggattaagc ggctcttaca
    35521 gtagtgcgct tcaagcggtt gaattgctga aggaagaata tgaagaccct gagatctatg
    35581 tttttgatac aaaagctgca agtattgggg aagggctttt agtattggaa gctgtaagat
    35641 tgaaaaaaga aggtaaaagc atcgaagaaa ccctagagtg gctggaaagt aattatttga
    35701 aagtccattc atgggtaaca gtagacgatc tgaaatatct agaacgaggc ggtaggattt
    35761 ccaaagcaag tgctactctt ggcagtctat tgagtgtaaa gcctattatt acggtggata
    35821 aagaaggaag gctgacgaat gttgacaaag ttcgaggaag aaacaagtcg atccagacaa
    35881 tcgtcaatga gacggtaaaa acaatcgttg caccacttga tcaaatcgtt tatgtggctt
    35941 acgctgggga tctagagagc gcggagaagg caaaacggct tttggaagag cagataaaga
    36001 tgaaagatgt gaagctttat cccttaggac caacgatcgc cagtcacaca ggctatggct
    36061 gtatcgcgat tttttcgatg ggaatcagtc gataaaagca gtaactcatt ctgtaaaaaa
    36121 gcccagaaat tgattgtaac aaaaacagcg actcttaaaa gatagatgag catcctatct
    36181 cttagaggtc gctgtttttt tattttttaa gctaaatagg ccattatgcc cataactcgt
    36241 tcaattgttt ttgaatatct gcattttcca aaaattcatc atatgtcgta tcagctcgat
    36301 cgatcacacc tttatccgaa acggcaatga tgcggtttgc caatgtttgg ataaattgat
    36361 ggtcatgtga cgcaaataag attgagccag taaatgccat caagccgtca ttcaatgctg
    36421 tgattgattc taagtctaaa tggttcgttg gatcatctaa tacaagtacg ttagctttcg
    36481 aaagcatcat ttttgaaagc atgcaacgga ctttctctcc accagataaa acattcactg
    36541 gtttcaatac ttcttcacca gaaaacaaca ttcttcctaa gaagcttctt aagaaggtgt
    36601 tatcatcttc ttctttactt gcatattgac gcaaccagtc taaaatcgtt agatcagtat
    36661 cgaattcttt cgttgtatcc tttggtaaat aagaattact tgttgtcaca ccccaacgta
    36721 ctgttccagt atctggcgta atttcaccca tgataacttt gaatagcgtt gttgttgtta
    36781 tatcattttc tgcgacgaaa gcgaccttgt cttctttgct caaattaaaa gatatatcat
    36841 ctaaaatttt ctttccatca atcgttacac ttacattttc tacctgcaat aagtcgtttc
    36901 caatctcacg ttcaggtttg aatccaacga atggataacg tctagaagaa ggttggatat
    36961 cgtctaaagt aattttttca agcatttttt tacgtgaagt tgcttgtttt gattttgatg
    37021 cattcgcact aaagcgggca ataaattctt ggagttcttt gatttgttct tcttttttgg
    37081 catttgcgtt tgcttgaagt ttagccgcta gttgactaga ttccaaccaa aaatcataat
    37141 ttcctacata aagtttgatt ttaccaaaat ctagatccgc catatgtgta catactttgt
    37201 tcaaaaagtg acggtcatgg gatacaacga tcacagtatt ttcaaaattg atcaaaaatt
    37261 cttccagcca attgatcgat tggatatcca gaccgtttgt tggttcgtct agcaaaagaa
    37321 catcaggttt accaaatagt gtttgtgcta ataacacttt taccttttgt ccaccagtta
    37381 attcactcat ttgctgatgg tgcaattctt cagggatgtt cagaccttgc agtaaaacag
    37441 ctgcttctgg ttcagcttcc catccattaa gctcagcaaa ttctccttct aattcagctg
    37501 ctcgaatacc atcttcgtca gtgaaatcct ctttcatgta aatcgcatct ttttctttca
    37561 gcacttcata cagacgcttg tgtcccatga tgactgtttc aagtacagta tagtcttcat
    37621 agtcgtaatg gttctgtttc aatacggcca tccgttcatc tggtcccata gtaacggaac
    37681 ctgtggaagg ctgcagatcc cctgataaca ctttaaggaa cgttgattta cctgcaccat
    37741 ttgcaccaat caagccatag cagtttccgg gggtaaattt aatattcaca tcatcaaata
    37801 gtttgcggtc tgaaaagtgc aaactcacat catttacagt aatcaatttt tttcctcact
    37861 tttatcatta ttagttttga caagtcttat agaattatat aggggaaagg taaaagattc
    37921 aaggcaatcg aacattcttt gtgtttcacg tgaaacagaa tgaaaaaaat aaaaataaaa
    37981 gcggagcgaa aatagctact ataggattta ttagtttgca acagcataat ttttgacgtt
    38041 atttataaga gggagtacta taaaactgta aaagaaatga aagaaggatt gaaaatggat
    38101 gaaaaaagaa ccatcgactg gggctcattg attttgggaa ttttatttgt acttgtctca
    38161 ttgttatcat ttcaagaccc tgtcggaaat ttagttgcaa ttgtagtagt ctttgcgatt
    38221 tttgccttta ttaaagggat atttgaactg tttgtacgga acagattaaa agagctaaca
    38281 ggatataaag gaaagatgcc cttaataatc gggatcatcg atattctagt tggcgtcttt
    38341 ttcttattca atattggtgc aggagtcgca gcattgcctt ttgtttttgc agtatggttt
    38401 attgctgatt cagttttagc gttatttact gctgacttgg caagaggagt aagtgaagga
    38461 tattattggt ttacgatcat cgtcaatatt ttaggaattt tattaggaat tttcttattg
    38521 tttaatccaa tttcttctgc gttgacatta agctttttag ttggattcta ttttatgtta
    38581 tttggtatca cacatatcgt ctatgcattt agataagtaa aatcaaacaa ataaaaaagc
    38641 cggcttccga ttacagtttt actgttgtaa ttggaagccg gcttttttat catggacaaa
    38701 tatgcacgag gtgattttta ggtgtcgaga caaatttatg caaaaatgtg agaagctata
    38761 tacacaaatt ttatcttaat actcgaagca tagcgcagtt gtcacaacct cttttcatat
    38821 aattcattca atactggcaa atcatggact tcatattttt ctttgaatgc ttggatttct
    38881 tcttcagaat attttcttgg atctaaatgc agatcttcta ctggcaatgg gcgcttgtta
    38941 ttgatcttca catcgattac tacaggacga tctgattttg cagctgcatc aaaggcaaat
    39001 cgaagttgcg agtattctgt gactgtaaag ctatttgccc cgagcgcttc tccaacttta
    39061 ccgaaatctg catcttctaa aaatacacca aatttctttt gttccgtatc ttcttgttct
    39121 gcttcgatga aaccaaaaga atcattcgaa aagacaacat taataattgg aagctgatat
    39181 ttgacttgtg taatgatatc ttgcatgacc atagcaaacc cgccatcacc acttaaggta
    39241 aatacttgtt tttctgggta gcttagttgt gcggcaattc ctcctggcac accattaccc
    39301 atagttgcga accatccaga tgtcgtatgt ttttgtttac cgttcatatt caataaacga
    39361 atagaatgga tagtcgtgtt accgacatct gtgacgaaaa tcgcatcgtc ttcagcaata
    39421 cgattgattt ctttatagac agcctctgga cgaatcggtt cttcctctcg atcttcaaaa
    39481 gatttcaacc aatttaccca tttttcttta ttttcttgat ttgctaggta ccagctatca
    39541 gcagggcgag catcccctaa ctcgacaagt ctttgcaaag ctgtgtttgc atcaccgagg
    39601 atcgacaaag atgtttcatg gcgtcgtcca aattttgacg catcgatatc gatttggata
    39661 aattcagcag ttggattaaa aaatgcacgt ccaaatggga agtcgctacc gacaaataag
    39721 atcagatcgg cttctgccaa tgcttcattt ccgggtttag tagcgacacg tccagcaaaa
    39781 ccaagaaagt tttcataatc atcaggaata attccttttg ctagaacgga agcgacaaca
    39841 ggaactgaaa aatgttctga gaattctttg atttgcggaa atccgtttcg cgttccttga
    39901 ccaacataaa gaactggttt ttttgctttt tcaagataag gcaaagcagc tagcaaatct
    39961 ttttcttctg gtaatatcac acttgttttg tgagtgtgag cggtggagaa aggttgttcg
    40021 tcaatttctt caaaaccaag atcaacaggg atagtcacga tagccacgcc tttatgctca
    40081 taggcggctt taatggcttc atctactaca tgcggaagac tttgtggtgt catgactgta
    40141 cgattgtata cgctaacatc tgcgaaaata ggattttcat ttagttcctg gaaagagtta
    40201 tagttcatgg atgtagaagc aacttgtcct aagaacgcta ctagaggcac atggtccatc
    40261 tgtgcatcat acaaaccatt gatcaaatgt gtcgcaccag gtcctgcaga gccgaagaca
    40321 gcgccgactt tccccgttaa ttttgcatct gcagcggctg cgagtgcgcc gacttcttca
    40381 tgacgaactt gaatgtagtt gatttcggtt ttttcttgat atagcgcatc cataattgaa
    40441 ttgaaagatc ctccgggaat gccgtaaata tggtcgatac cccagctttc aagaactttt
    40501 accattgcga ttcctgcatt gatttttgac atggtgtttc actcctttag caatgatgat
    40561 acataaattt tttgtatcta gagtatagaa ctttttatcg aaaatcccaa ctaattcgtc
    40621 ttttcacaaa aatgtagtca tgtttttggg aatatgctag agtagtacat agatagattc
    40681 aagagagggg aatggcatgg ttgaatggct attgagactt gaaccttggc agcaagcact
    40741 tgctgggaca gcttttactt attttatgac agctcttggt gccgggttgg tttttttctt
    40801 taaagaaatc aaaaaagaag tgctaaactt gatgttagga tttgcttctg gtgtcatgat
    40861 tgctgcaagt ttttggtctt tgttagatcc ggctattaca aaagcagaag aaaatggaga
    40921 tattgcttgg ctagttgtaa gtattgggtt tggtttaggc ggtgtgtttt tatatatggc
    40981 agataaaaca ctgccccata tgcattttgg accacaacat gaagcagaag gattgcctac
    41041 ccatttaaaa cggacgatct tgcttgtttt ttctattaca ttgcataaca ttcctgaagg
    41101 tttagccgtg ggggtagcat tcggtgcagc agcaactgcg gataatccga cagccgctat
    41161 attagccgct gtttcggtgg cgctaggtat tggtatccaa aacttcccag aaggagcagc
    41221 tgtatcaatc ccgttgcgac aagaaggtct gagtcgtaaa aaggcctttg tttacggaca
    41281 agcttctgga atcgttgaac caattgctgg agtgattgga gcgctactag taacgaaagt
    41341 ggagctgctg cttccttatg cacttgcttt tgccgcagga gcaatgattt atgtcgtggt
    41401 ggaagaattg atccctgaag cacagcagac attgtccagc aaacgtcatt ttgcagtttt
    41461 tggcgtcatg agtggattta tcattatgat gattttggat gtggcattag gataaataaa
    41521 aaaaatatat atgatatatc aaatagggcc gcgaacttat atcgcgaccc tattttttta
    41581 gtaagttata tcataatgaa gtgaatggta tcatacatta ataatagttt tttctatgtg
    41641 tatttccttt gatccagcga acaagccaga tgataagtgc gattgggatt agaactttga
    41701 ccgcaaacca taaaagcccg cttaggacac tgacaaaaag actgatgacg aagctagctg
    41761 caattagcac taaaagaacc atgaagatat ttaagaacat atttttttga cgcatacaaa
    41821 accctcctct tgttcattct ttactataga aaaagtataa cagggattgt ttaaaaacac
    41881 ctccaactga agactgattt ttacttcatc tacttaaatg atcaaacaat tgctttttct
    41941 ctggtttatc atctaaaata gagcggaaaa ggaggagaat atcatgccta aaaatcgtct
    42001 tgaagcattt acagatgctg taatcgcgat tatcatgact attttagttt tagaacttca
    42061 tgaacctaac ggagacacgt gggaggcgct cgctgcatta ggttaccgat tttttatcta
    42121 tattgtcagt tttatcattt tagcgattta ttggaacaat catcatcatt tatttcaagt
    42181 tgtacataag ataaatggac gggtcttatg ggcgaacagt ttctttgttt ttgcgctttc
    42241 gctttttccg tttgcaactt catggatcag tgagtacatc aatagcctgg ctccagaaat
    42301 gacttatggt tttgttacct tgaccgctaa tattgcgtat tatatcttgg caaaagaact
    42361 tgtcaaagca aataaaggcc acgagaaagc attgaaggcg atgttgaata attacaagaa
    42421 atcgtatcta tcgattggtt tgaatgtgtt aggaattgtc ttagggtatt tagtagcgcc
    42481 agtagccgtg ctgatcgcaa acatcgtgat tctagctgct tggatcatcc caagtcgtac
    42541 catcgaatca caatatcggt aaaatgatcc gtgtttaaaa tttaagagaa aaatatagaa
    42601 aaaataaata aacgatcaaa tatccgagtg atactagtat tcaaagaggg atgcagatgg
    42661 atcaaaaaag aattcatgta ggactgatta ctagttttgt cacgatgctt atttatttgg
    42721 atcaaggtca tttgacttgg acagtagttg gtgtcgtagg atttttgaca ttattcacag
    42781 caataaaaaa ataaacatct ttatgtaatc tgatgttcta tctatttaag aaaagcatgt
    42841 ttacagcagt atgattctgt tgtaaacgtg tttttatttg tggaaaattt aatttttaga
    42901 aaagtgtatc gaaaaataga tagttagttt ttccacagtg gataacttcg taagagataa
    42961 aaataaaaaa ttagtcaaag aagggcgtat agatttcttt tgataggaga tatacaacag
    43021 aaatgaataa atattaacgt gttttcaaac agtataaaac ggtgtggttt ttggaataaa
    43081 aaaataacat atctcatttt acgcttttgt tattttatac ttaaaaaata acgacacaga
    43141 aaataccgtg tctcagattt ctctactact tctaaaaact actgtttttt tcctttgaaa
    43201 tgccttcaaa aagtaggatc aggcagaata tactggcaga atcaaggaga agggagccta
    43261 aataaggaga aaaaccatag aaaatataaa gtaattactt ataaaacaag ctttttatag
    43321 aaaaataata tacctgagta gggtataaat taagacggaa ttcctttcta gattgagata
    43381 ttgactagga agaatgttaa gattttaagc ctttaagcta aaaaaaaacc ctttttgtta
    43441 aataagggga atatagcctg tcagtttcaa actgcaagtc gtcctgctgc aataatatag
    43501 tagattagag atgaaaatag atttttttaa accaatgact gaataaaaag accgtcatag
    43561 atacacattg gaatgtgtct attacggtct ttttttacga acgattttat tgaagacgga
    43621 cagttcgggt caaaaataaa aaagctacga tcagaaataa gaataaaatg tatcccaaaa
    43681 taatggctag acggtctaac ttatttgtcg atagatgttt gtttagaagt tgataggccc
    43741 ataaacctat cagtcctcct gttgtattag ttatcaaatc agtgatgtcc gctacgccaa
    43801 taccgaatag gaattgaatc aattctgcca agaagcttac aaggaaaata atccctaatt
    43861 ttttagaaaa agaaagtctc ttgaaattga cattcaaaag caccccaaaa ggaaaaaaga
    43921 caatcaagtt ataagtggtc tcacgtaata ccgtcgtatt ttcaaaagaa aaagtagaaa
    43981 aagggacgag attgacattg ctgtaagaat aagtgagaaa ttgtggaaga ttaaatgata
    44041 gcttgaataa aatcaaccat aatagaaaga ataaataaat ccctaataaa aattttgata
    44101 cataccgcat agacatctcc tccatttaaa aagtatacca tcgaaaaccg ctgaaaaatc
    44161 ataaaaaagt atgaagttta aggaccaacg tagttatcgt acgaaaatta gaagcttttt
    44221 tttgttggga atccggtttc tttaacaaag ctaagatagc gagttgcttg gaaaagttgc
    44281 tatctttttt ctgtcaaaac gactcggaat gttcgttttt tgttctaatt tctccaagtt
    44341 gtttactttt tgatactaag tttatgaaaa atcaagaaat agttgttata ctaggtaatg
    44401 ctgacaaacg ggattggcat ttcatcttgg ttcagctttt tttccaatta ctaggaagga
    44461 gggatagatt gttcaagact tattttcgtt tcaataaaat acaagataat ccttttcgta
    44521 tcatgaatct gatgctactt atctttattg tattatggtt aggttacata aatcatgatt
    44581 ttttgatttc aagtttttca tcattaggga tctttacttt tttctattat caaaacatac
    44641 cgatgaaata tttgctgaaa cgtatgtttc tgatcggttg cggactgttg gttgctttcg
    44701 ttttggggat cttatcgact tatgttttat ggcttgaacc atttgccgta gctttggttg
    44761 ctttttccag cagattcgtg ttgcgattat tccatatttc taaaccaggt ggcttgtttt
    44821 ttgcgatgct ttctgccatg ggaacgagca tgcagctgcc aatcgctcaa ttaccgatcg
    44881 tctcacttta tttttttatg ggtgtcgttt ttgccctgat tgcggcagtg attacaaaat
    44941 tactggattc acggcctgag cagacaattg aaaaagcaac tttaaaagaa agatttcacg
    45001 aagagccttt agtgatcatt gattctgttt tctacagcgc tgccttattt ctttccgttt
    45061 atgtcagtca cggcttgaat ctgcataatc cttattggtt gaccttgtca tgtgcatcga
    45121 ttttgctagc agaaaacttg gatgcgatga agcatcgaca agtccaatat ctgattggat
    45181 cgatgggtgg gttatgtgtg tcggcattct tgtcatttgt gccgtttacg cagttgcaga
    45241 ccatttttct gatcactttt ctatatggaa tcgcacaatt tctagttgct agaaattatg
    45301 cagttgctaa tatttttctt aatccgatgg cgttgatgct atctacactt attcgtggag
    45361 catacttgat ttctttgatc gaatatcgtt ttttagggat cgtcatagga agtttcatcg
    45421 gtttaggtgt tgcgtgggta atgacggttg gtttgcaaca ttatttaacg gttgttcgtc
    45481 aaaaattaga atagaaggac aaatatatgt gccaaaagag gttatgacaa gatttttgtc
    45541 acaacctcta attcaaggtg ttcaaaagcc agattctcag cattaagtca aattcacaca
    45601 aaaatgtgga aaggaacttt caaaaaaatt ttatatttat tctatcaaaa aattttgttt
    45661 atgatggtaa ctgaataaac tttcctgaga tggtcaatgc tttccttgaa agtcttacag
    45721 atattctttc ttcatagaaa gtttaagttt tatagttagt gtacaataaa agtatgttta
    45781 taccatatgg tgtccggata atcagtcatt taaacaatga ggttcaaaga tatgaaaagg
    45841 ggaaatttga tgaagaaaat aagcaatgaa atccaaattc tggcagaaac tttaactctt
    45901 cccaaagtcg ttatagagca aatgattgta caagcaaaaa acatccttga tgaggtgttt
    45961 gagcaatata gtcatgaact aatggatcct aataaagcaa aggcgactgc aaagaaaata
    46021 gacaaagtct atgaagagga accttttgca gcattagcta tttatctata tgcagcttct
    46081 caaagttgga aaaaaattta tcaaccattg aagattccaa gaactatcta tttggcaaca
    46141 atgaatgctt ttactagatt tattcatgaa catttccaag taacaggaag ctatcgcttt
    46201 gaccgtggtt tttggatttg gcattacact agtggcttga tctttcggat tggtgaattg
    46261 gagtacgaaa tgacatattt tccgaaaggg tacacggatc taagattaga aggaaagcag
    46321 tgcctttctc ttcatattcc ttccgatgca gatttatctc agaataagat aagagatagt
    46381 tacgaaagtg ccattcgttt ttttgaactt tattttcctg attaccatta ccaagtgatg
    46441 tacacagata cttggttact atcacccaat cttactaaat ggttgaaaaa agagtcaaaa
    46501 atctgtcttt ttgcagccga ttatcgatta ttatcagttg atgaacagga cgatagtgga
    46561 gtcccttgga tctttggacg agtagatgcc caaatacatg attatccaga aagtacgagt
    46621 ttacagcgac aagcaaaaga gcaattgctt gcaggcgagc acattggctc tggcttaggt
    46681 attcttactt cataagttta gtacttcgaa atagtttaaa agagcgtgag acaaaagtaa
    46741 agaacacttt tgtctcacgc tctaaacacg tataaacggc ggaagcagaa gcaactcctt
    46801 cggaaataag ctgaaattca caaaaatttg aaaagcaatt ttcgtgaatt tcttcttatt
    46861 tcttggagtt aaacgcttct gtcccagcct cttttttagt gttctatcga acacacagta
    46921 cgttcaatag gtaaactagc tataacaata gatattaaag ttaacggaca agagggcctt
    46981 ttttaggtag ctaaaacgta ataatgaaaa tttatttcca gagataggag tcaatcagct
    47041 agtctgatcg actgttttaa caagttgact ttcattttaa cttttgatac catttaaaac
    47101 acaaatcgta attgtttcga tttgtgtttt ttagcatgac tacaatcaaa gaagaaagtg
    47161 ggtcgtccat atggaaaaaa cagatatctc aagtgcatac cgccgcttga aaagcccaaa
    47221 tatcaaaaca cgaaaacgtg cgctgaagat catcaaagat gtgaaacgga atagtgggaa
    47281 aaggtaagaa gaaggaaact taattaaaaa aatagccgaa aaacgttttt cggctatttt
    47341 caataatcgg tttttgtgta aggtttccga aagaactgct gttgattttc catttttaac
    47401 atatgataaa ttggtagaat ctttcttttc aaattgatac cattccctaa ttccagttat
    47461 atagttaact acagaaccac aaagcacgta cgtttataaa agtattatct gtagttattt
    47521 ttcaattccc tttcaacctc ttattttgac cagtagtatt gatcacttta ccttttaaca
    47581 cctttgttcg tttttgaaac atttttagtc aagaatggaa acgtaactac ataatcaaag
    47641 gttttgcgaa atatttcatg aaatatttca tgtagaagcg agcaatacgg ttgcaaataa
    47701 ccattatcgt ttaataagca gttacgaaag ctcccaatat ctctaacgtt cagctcactc
    47761 catttctttt cttcaaaaaa atcagtgatt cgtatctctt tctatttgtc gaaaaagtta
    47821 actttaaaaa gtaacgccga aaaaatatgc aaaaaaataa aaaagcagtt actattgcta
    47881 gaataacatc aaggaataat aagttttggt tttcagataa tagtcgataa gttgtgtatt
    47941 ggaattttag atacagtaat aaagtatttt tcatgaaatt ttgtgagaat cactattaca
    48001 agtatcttta ataaaattaa tggttaagga ggaacttaaa gtagaatgta aatgaatagt
    48061 tgtagtcagt cgattgtgat actagactca aagttgcagg aataagtttt tcaatttgtg
    48121 attggaagat aaaaaatgaa aaagaagggc ttttttttat actttgttga ttgtagtgtt
    48181 tattttccct ctgtttagcc aagtaacatt ttctatgact caaccagttt caagtgagga
    48241 acatgataac ctttttcgtg ctacagaaga gtttaagaaa tatcaggaaa atattgttcc
    48301 ggagactcca ttagctagtg agccaattac tgatattgat ggaaaccaaa taggatggta
    48361 tatacagtac gaagttatgg tggtaaataa tgactcaata gtaaaaaata cagtaggctt
    48421 agagctaaca ccgattgaaa aggtgagttc attgttaacg tatatttata ctgatgaaag
    48481 tgacgagact gagatcattt taacggatta ctcaaaaatt gctactgata aaaagtatat
    48541 attgtggatg tagacactag tgaaatactt tatgatatag aaattgatga aaatttacta
    48601 gatgtagcac aagaaatcga tcatcaaaag caggaagcta ttcgatatgc tggtagaaaa
    48661 gggaaaagaa aatatttatt acgtgaatgt agcaaaagtt agagaagatg aaaatgagtg
    48721 gaaagaattc aagagtcgct actcaattaa ctcgactccg acatttacag tttacagaga
    48781 aggatcgata gaaaaaactg ttttttggac taaagaaagt ggaatgtctt tagctgaagt
    48841 agaagagttt ttggattatg ttagtatgca acagtaaggg agttcttatt atgaaaaatt
    48901 ttgacacata ttatgttatt gttttttcga tatctttgct gttaatatta ctaactttaa
    48961 tgcagtataa agagtatcca gagtatttta ttttaacttt tgtatattta gtaagtagct
    49021 acacacttct atataaattc agaagttctt ctagagtttc taaagtttgt ttcttaatta
    49081 gcttattgat tctataatat ttttgattta tataggaaat taaaaatatt atagaatcaa
    49141 cactcagaga acgggttggt ttttaataga tttaataagt actgataatg aaatttctcc
    49201 tggttgggaa aacgaagttg gttaccctgt agaagaattt tttcaagatt actttgcgga
    49261 gtatgccgat aggataatga gagattacgt aacagataaa actcaagaag tcatcaatca
    49321 atcccagtca ttaaatgaat tatctgataa ggcgaaagaa gaagcagttg ccattatttc
    49381 ttcagggatt agtgactata ttgatatgag tggtgaaatc aattatggcg ataaagacaa
    49441 aattgtacgc atgataaaag cttttatcaa aacttttgat gtgaaggcag atcttaagtt
    49501 taatgatact tacctagtag gaaaattaat taatatcctt gggacagaga aagatacgga
    49561 aacgtttatt tcctctttgt ctatgttgtt tacagataag catttttcaa ctcagcttgg
    49621 tggagaggaa ctagtggaaa tcttttaacc attacgccca gcattaataa aagtatatca
    49681 aaaaagtact cctgatgatt tttaagattg gtttgagaaa taatgtgaaa acactatttt
    49741 taatgttaat tatagataga gatgaacgcg tcatatggtt aaaagaatga aaaagtcaaa
    49801 gatacactgc tatatttcac taaggatata gcagtgtatc tttcatgtcg tttcactggc
    49861 agttttttat aaaaggaggt gtgcatttca gttgcttttc tttggtagtt agttgaatcg
    49921 tttaagaatt aacggatgtg gcagacaggt gctctgtcca aaaaccagta aaatctttga
    49981 tggttccatc tttatttagc tgtgcctgtt caaatactgt tttgttttta ccgaatggat
    50041 caattgcaat aattgttccg atccccgcac gtttggcagc aagtaatcct gagtaagcat
    50101 cttcgataac aagacaatca gccggagcta agctcagttt gtctgccgct ttaagaaaaa
    50161 tatccggttg cggtttccca gggaaagagc cgtcgtcata gacaatcttg tcccagtcaa
    50221 accagcgggc taaatcgaaa atatcaaaat aaaaagcaac attctctttg acagtagccg
    50281 tggcaatcgt taatggaatc tgttggtcag tcagctggtc aagagtcgct tcaagccctt
    50341 ttgtcaactg aagctcgttt tcattttgca agcaaagcat acgataatag ctttcttttt
    50401 cttctgacaa tacggcgatc tcagaggcgg tcaaatgctc tgaaatgaaa tgtttcaaaa
    50461 tctccgtatt cgtccgacca tgaatatttt ttaaaatatc ctcgtctgat aaaccatctt
    50521 ttgtatgatc atgtatcata tgcagccaag cagcttcatg cagatgagag tccaaaaaca
    50581 ttgttccgtt gaaatcaaat ataatccctt tcatagttac gctcctatag tggtattttc
    50641 ggttgaatca gcaatttgca gatcgacata ttgtgcatag tgctcttgaa cctcccgcga
    50701 gatctggcga tccgtaatga gcagatcgaa atcatgcaaa ttataaaaca cataaaaatc
    50761 atctcgattg aattttttat tatcaacgag tagatatttc ttacgggaat tatttagagc
    50821 gatctgctgt gcttctcctt cttcggtact gtaagtagag atcgcttcgt tgtgaacgcc
    50881 atttgaactg ataaatgctt ttgtaaactt tagtcttctt aaattgtcgt tggtgatcgg
    50941 tccgacaaat gcgccggtat ttttccggaa atcaccgcca atcagtagaa tttctggtcc
    51001 atctgcttga gcaagaatat caaaaactgg ataattattt gtaatgatcc gaattttctt
    51061 gttcagtaaa tgcgtcgcca atagttcgat tgttgtacct ggtcctaaga aaatcgtttc
    51121 tccctcgtga atcaaggagg ctgctgtggc agcaatttct tttttttctt gaatctgcag
    51181 agtggctttt tcagtgtgag aaagttcttg atctaaagtg taggaaagac tttgagcccc
    51241 tccatggaca cgcagcagct tgcccgattt ttctaattca tctaaatctc tgcgaacggt
    51301 catatcagaa acatctaatt gattgatgat ttcattaacg gtaaggattc cttttttatt
    51361 gaccatttct acgatagtaa gcaatctttc ttttttcaac attgtcttca cctctaattt
    51421 aaggataatc tatcatataa acggacaaca gtcaatctaa ttcgaacaaa agtaaacatt
    51481 tagggtggtt tttatgtttg ttacaataga ttttattgct gttttctggt ttttatgatt
    51541 atttgtgttt gtttttgttg acaaatgttt tggatgcgtt tacaatgcaa tcagttagaa
    51601 agagcgaaaa ctgattatag atttgaggag agatgtacat gcttaaatta ccagaagatt
    51661 ttatttttgg cggagccaca gcagcctatc aggttgaagg agccactaag gaaggtggca
    51721 aaggcgctgt tgcttgggat gactttttag aggaacaagg gcgattcagt cccgatccgg
    51781 caagtgattt ttatcatcaa tatgcaaagg acattgaatt gtgtgaacgt tttggtgtaa
    51841 atggtctaag attatccatt gcttggagcc gcatttttcc tgatggagct gggaaaccaa
    51901 atccagaagg aatcgctttt tatcatcgtg tgttcgaaga atgtaagaaa agaaacgtaa
    51961 cgccgtttgt gacgttgcat cattttgata cccctaaacg tttgtttgac catggagatt
    52021 ttttgaatcg agaaacaata gaagcatttg tttcctatgc cattttttgt tttcatgaat
    52081 tcaaagaagt caaggtgtgg agtaccttta atgaaatcta tccagttgca accaatcaat
    52141 atttgttagg tgttttccca ccaggaatca aatatgattt tacaaagatc gtcgcctgtc
    52201 ttcataatat gatggttgct catgcccgtg tagtgaacta tttcaaagaa aacgaacttc
    52261 cgggtgaaat tggtgtagtt cactcattag agacgaaata tgcggcaaca gacgcgccgg
    52321 aagataagca tgctgccttt ttagacgatg cattgtcgat ccgtttctta ttagatgcta
    52381 cctatttagg ctattattca actgaaacct tgactgctct tgatgagata tgtgaagcaa
    52441 atcaggccag ctatcatttt cctgaagaag atttcgttga attgaaaaaa gcatccacgc
    52501 gtaatgacta cttagggatc aatcactacc aatgccattt tgtcaaagct tacgatggag
    52561 aaaatgcaat ccatcataac ggtacgggtg aaaaagggac ctctgtttat aaagtgaagg
    52621 gaatcggaga acgtatttat aaagaaggaa ttcctcgaac tgattgggac tggctgattt
    52681 atccagaagg tctatatgac ttgttgctgc gaattaaaag cgattaccca cattacaaca
    52741 aaatctatat cactgagaac gggatggggt ataaagatca gtttgaagat ggcattatta
    52801 tggatcagcc gagaatcgac tatttgagag tctatttgga atccttaagc aaggcgataa
    52861 cagccggggt aaatgtgaag gggtattttt tgtggtcgct tatggatctt ttctcatgga
    52921 caaatgggta taacaaaagg tatggcttgt tttatgttga ttttgaaacc cagaagcgtt
    52981 acccaaaaga atcagcttac tggtacaagt tggtcagtga aaccaaaacg attatttaat
    53041 aagtgataat ggaggaagaa aagatgaata aagaggaatt acaaatgctt ggttttgaga
    53101 tcgttgcata ttcaggcgac gctcgcagta cattattgaa attattgaaa gaagtaagac
    53161 aaggaaactt tgaacatgtt gattcggcgt tgaaagaagc cgacgaaaat ttaacccttg
    53221 cacataattc gcaaacaaaa attttagcgg aagaagcatc tgggaaagag atggagatgg
    53281 gattcatttt tatccatggg caggatcatt taatgaccac tttattgctg agagatctga
    53341 ttcaagactt tattgtactg tacagacaga atcaaggata ggagaagaac gctatggatg
    53401 gaatcattaa acaaatcgaa aaagggaaac cattttttga aaaaatctca cgtaatagtt
    53461 atttaggagc cgttcgtgac ggatttttaa cagcgatgcc agctatttta ttttcaagca
    53521 ttttcatttt gattgctgcg gttcctgaga tttttggtta ccaatggcct gaatctgtct
    53581 ccacatggct atggaaagtc tataattatt cgatgggtgt tgtagggtta ctcgtttcag
    53641 caaccactgc tcgctgcttg gctgattcga tgaatcgtcg cttaccgaaa aacaaagtaa
    53701 tcaacaccac ctctgtgatg ctggcctctg tggtaagttt cctgttgtta agcgtctctc
    53761 cgattgaagg tggattttca acggaatata tggggacaaa gggactactg gcttcctttg
    53821 tcgctgcatt tttaacagtt aatttatata agttttgtgt gctgaaagat attaccatta
    53881 aaatgcctaa agaagttcct ggaacgattt cgcaaacctt ccgagatatt tttcctttct
    53941 ctttttcagt atttgcggcc gttttaattg atttaggcat tcgacaaatc ttccaaatct
    54001 catttgcaga agcaatcatt acggtgctgc agccgctatt tacagcagct gacgggtact
    54061 taggaatcgc tattatctgg ggagcgatgg cgctgttttg gtttgtcggt gttcatggac
    54121 cgtcgatcgt ggagccggca attgcggcaa ttatctatgc caatgtggaa accaatctgc
    54181 aattgttcaa ttccggtgaa caagcttcta aagtactgac ggtgggttta gggaattttg
    54241 taggaactat gggaggaact ggggcaacat tggttgttcc ttttatcttc ctactctttg
    54301 ctaagtctaa gcagttgaag gcagttggga aagcttcctt tattccagta agtttcgctg
    54361 tcaacgaacc attgctattt gcggcaccca tgattttgaa cccttatttt tttattccat
    54421 ttttgttaac acctgttgta aacgtttgcc tgttcaagtt ttttgtcgat gtattgggca
    54481 tgaacagctt tatgtacgtt ttgccttggg caactccagc accgatcggt ttggttctag
    54541 gaacgggcat gggtatctta gcttttgttc tagcagcggt tctaatcgtc gttgacttcc
    54601 tgatctatct accattttgc aaagcatacg atgctgtctt agtagagaaa gaagcacaaa
    54661 aagcagaggt gatcgcaacc gaggaaggtg cttctgccga tgtacgtgag gaagtacctg
    54721 tggcagcgag tcaagttatc agcgatgcaa ataaagaaat caatgtgttg gtactttgtg
    54781 ctggcgcagg gacaagtgcg atgttagcta atgctttaaa aaaaggtgca tctgaatcga
    54841 aagcaccgat cagtgcaatg gcgggtgctt atggttctca ttacgaaatg ctgcaggatt
    54901 ttgatatggt ggtcttagca ccgcaagtca attcttacta tgatgatatc aaagaagaca
    54961 ccgatcgctt aggaatcaag ctggttgcga caaaaggagc agaatatatc ggattaacga
    55021 gagatcctga aaaagctgtt cagtttattc tgaaccaatt tcaataaagg aggagccata
    55081 atgaaaatgg gaatttggtt gcgtaaagct gttttatttg cagctgtgtt aggggctttg
    55141 ggggctgttt caaaaactgt tgaagcatct gccagcgaat ttattcgcgg agcagatatt
    55201 tcgatcctgt cagatatgga aaaatctggt gcaaaatatt atgaagacga tatttcaaaa
    55261 gatgccttag agattttaaa aaataatggt gtcaactatg ttcgactgcg tttgtggcag
    55321 gatccttata atagtaatgg agaaagttat ggagcaggga caaatgatct ggaaacgacg
    55381 attgaccttg caaaacgagc aaaaaatctg ggacttaagg tgctgctgga ttttcactac
    55441 agtgattttt gggttgatcc tgggaaacag aatcttccta aagcatggca aggtctgacc
    55501 tttgaagaaa tgaatacggc attgtacgac tatacaaaga acgttctgtc ggaaatgaag
    55561 cagctggacg tttatcctga tatggttcag atcggcaatg aactgaatag cggaatgctc
    55621 tggccttatg gcaagtcttg gggagaaggc gggggagaat ttgatcgatt ggctgctttt
    55681 ttgaagtctg gtatccaagc tgttcgagac actcaaccta aaacaactcc agtaatgctc
    55741 cacttagctg atggtggcga tacaggagca tttacttggt ggtttgatga aatcacgagt
    55801 cgaggtgtgt catttgattt aattggagtg tcgtattatc cgtattggca cggttcaatg
    55861 aatgaacttc aaaccaatat gaatgcgatc agccaacggt atggtaaaga agtgatcgta
    55921 gttgaaacag cttatgggca tacaacagcg aatgccgata ctatgccaaa tgcttttggt
    55981 gaagccgaag cagctgcggg gggatatgaa ccaacaccga caggacaagc agagtatttg
    56041 ctggatcttg ccgatcggat tcaggcagta ccgaataatc gcggggcggg cttcttctat
    56101 tgggaacctc tttggtacaa cggaaatgtg agttgggcaa cacaagcagg tatgaattat
    56161 ctcagtgttc aatccactat gggaaacgaa tgggacaacc aagcgatgtt tgattttgct
    56221 ggaaacgcct tgccaagctt gcgtgcgttt aaacaagcag gggcgcaaat aaatctagtt
    56281 aaaaatgcaa gtttcgaaag cgatggacca acgaccagtc cttccaattg gcaaacctgg
    56341 tttcaacaag gaaccgctaa gggtacgatc aaaacggaag cgggcacttt ttttggggac
    56401 tacaaactaa ctttttggca ggatcaagca tacgaggcat cagcttatca aaatgttagt
    56461 ggcataagca aaggaactta cacattgagt gcctatgtaa tgtctggggg agagtttgat
    56521 actaatcagt tatatatcaa aaattatggt ggagcggaac taaatactgc ggttgttaaa
    56581 acaagtgaat ggacaaaaat tacaatcaaa gacattccaa ttacaacagg tgtttgtgaa
    56641 attggggttt atagcaaggc ttcaggaaat gcttggacga acgttgattt tgtttcattg
    56701 gtgaaacaat agactgcgga tcgtttcgat tcaagatcgg aggttccctt cagccacatc
    56761 gttagcgtag aaggcaggaa tcgaacctgc acctcgcctt tgccaaaaat aaccagaaac
    56821 gatttccagt catttttacc aaaacgagta aagcaccaga cttcatctac actttgtatt
    56881 atgctaaaga gagttcaacg atttcgtaag aagcaatgaa aaaattgcaa aaagttgtgc
    56941 agataacata aagtttgttt aagaaagggt aaccttagaa ttccgtcgtg aatatgattt
    57001 gagcacttag atggatagaa aaaagttctc ggaaaacagt tcgtttttcg agaacttctt
    57061 tttatgataa gaatgaaaca aattaactta tattatagtc tcttttggta gggaaccatc
    57121 ttatttttaa tgaaaatcaa gcattttagc tattgaagaa tgttgattta tagggattcc
    57181 ccctccatat tatgttggct ctttattccc actctgtttt caacgtaaag tgtataaaac
    57241 agaatgcgat actatcaagg gagttaaaac aatatacaga acagtaaaac actataaata
    57301 aaaataaaag tgcaataaaa gtgcaatttt tgcacctaat catactctgt ttatatatga
    57361 aaactagatt ctgaatccaa tatttggtta acgagaggcg ttcgaatttg aacgtctttt
    57421 atatattact ttcaaataaa tatgaggtag aatactatct attttctttt taacccaatt
    57481 gttataatgg agagtgtaaa atattttgtg taaatagaaa aaaggaagtc ccttctgtag
    57541 aatagagtta ccacaacaca ttcacagaag agaggacttc cctatgaacg attttactac
    57601 agaaattcta aagactctag cgaacaaagg cgatttgaat gaattattcc gtgtccattt
    57661 ggagaaagca gtcaatacgc ttctcaaaac ggagttaacg gctttcctag attacgaaaa
    57721 gtatgatcgc attggtttta acacgggtaa ttctcgtaac ggctcctatg accgtacggt
    57781 caagaccgag tatggggaac ttcatctcca gattccgcgc gaccgcaatg gtgagttcaa
    57841 gcaacagact gttcctgctt atagacggac gaatgacacg ttagaggaga ccgtcattca
    57901 cctcttccga aaaggtatta ccatgtcgga aatcgcagac ttgattgaga aaatgtatgg
    57961 gcattactac acgccccaaa ccatgtccaa tataacaaaa tcatttacag aagaggtaac
    58021 ggcgtttaat gggcgggagc ttcatgaccg ttatgctgct atttatatgg acgcaacgta
    58081 tattccgtta aagcggaaaa ccgtcgccaa ggaagctatt catatcgcag ttggcattcg
    58141 cccggacgga tcaaaggaag tattgagcta tgcgattgca ccgactgaat ccatcacgat
    58201 ttgggaggaa attttattgg accttcaaga gcgcggtttg aaaaatgtcc tcctgttcat
    58261 cacggatggc ttaaagggga tggtaggagc gattagtcgg ttctatccca aagctcgttt
    58321 tcaacattgt tgtgtacacg tttcccgtaa tatcagtcat aaagtgcgtg tcgatgatcg
    58381 taaggaagtc tgtgatgatt ttaaaatggt gtatcaagcc tcatctaaag aggtggcgtt
    58441 ggaagcacgt ggtacttttg cggaaaaatg gaaaaccagc tatccaaaag tggttgaatc
    58501 gattctttcg aacgatcact tgctcacttt ctatgatttc cccttggcca tacgcaagag
    58561 tatttattct acgaacttga ttgaatcctt taataaacaa atcaagaaat acagccaccg
    58621 caaggaacag ttccaaaatg aagagtcgat ggaacgtttc ttagtctcgt cttttgatac
    58681 ttacaaccaa aaattcctag gtcgcagtca taaaggtttc caacaggccg aaggcgagct
    58741 cgaacaaatg ctaagccaac tgattgagaa ttagaaacaa tctgcagtag ggaagaatca
    58801 tttacacaaa attattgacg ctcccttata atgagaactt ttactaacat aatttatgat
    58861 aaacttacta tataatcata ttaattaaat agttaggaga tctgcttatg tttatatcta
    58921 ctcttcaaat tcgaaatttt agaaattatg aatccgaaat ctttcgattc gataatgaga
    58981 caaatactat tataggtgag aatgattcag ggaaaaccaa tgctcttact gcattgagaa
    59041 tcttattaga tgatagttat tactatagta gtaaaacact aaaagaatca gatttttttc
    59101 atggaattca aaatgggtgg caagggcatt ggattattat ctctgcgaca tttgaaggaa
    59161 tcagcgaaga agaatttgac aatgagattt gtgcttccat ttctttaaat tctgaaagtc
    59221 aaacaatctt agaagaattg atttcaaatg cagataaagg tgtaggtagt atttctctat
    59281 ttattagacc taacaaagct attagaaagc aattatttga tatttccact gaattagact
    59341 ctcatcaatt taacgaattt agaaattcta ttcgtctatc cgactatgaa ttttactaca
    59401 catctaaatc aaatctaaat ttctgtatca atgaaaatta tgataaattg gtaggaagac
    59461 tcaatgacaa tatagctagt aatcctgaag aagacgatga agctctgttg ggctcaagaa
    59521 ttgacatgtc agatattttc aagcatattt cggttgttta tgttgacgct cttcgagatg
    59581 ttcttagaga aatgaaaaac aatcgaaacc cagtcaaaag aatcatggaa acaattgaat
    59641 caaaaatatc atcagataat gttgagtcat taaaaacaat tatacagcaa ttaaatgaga
    59701 ctattaccag tgttcctgaa attaggaaaa ttggcgaaaa tattaatcgg caactaaata
    59761 gcataattgg ttcagtgtat tctcctaatc ttcttctttc ctcaactatg tcagatgata
    59821 tgggttcttt agcaaaattt ctttcaatga agccagaaca aaatatcgac ttagacttac
    59881 tagggttagg acatctaaat atgatttatt tagctttgaa aatagtcgaa tttgaagcgt
    59941 gtcgttcaag agaactactt aatattatgt tgattgaaga accagaagct catattcatc
    60001 atcatattca aaaaactctg ttcgaaggat taaatcttca aaaaaactat actcagattc
    60061 tcatgaccac acattctgta catttagctg aagtgtctga aatatctcgg atgaatgtat
    60121 taaaatcgtt taatggcaaa tctatcgtta tgaatccagc aaataatctg aactcatttg
    60181 ctaaagaaaa gttaaaaaag aacaatctaa atctaataaa cgcagtagag agatatttag
    60241 atgttaaaag aaatggattg cttttttcta aaggtgttat cttagttgaa ggagatgctg
    60301 aagaaatact tattcctcaa atagtaaaaa aattatttgg tataaactta gacgaacttg
    60361 gaataggttt gattaatgtt ggtagtactt cgtttgaata tatcgctagc ttgtttgacg
    60421 atgatagaat acagagaaag tgtgctattg taactgattt agatgaacag gcaatcccat
    60481 cagaacatcg attgtataaa agcactgcac aagagaaagg tcaagcaaga aaagaaaaat
    60541 tatcctccct ttttaatgaa aacaattggg ttaattgttt ttatgctaat acaacatttg
    60601 aaattgagtt tttagatgtg aataaagaaa atattctaac ttatgtcagt ccaattatag
    60661 atgacgcatt caaagataaa aaaacaataa ctgcttataa agaagaaata caaagtgaac
    60721 aatatttaga aagaaattta gcgatgttaa aactagccaa tcatgttggt aaggggtggt
    60781 ttgcaattga gttaggtgaa aaaattgact ttttaatcaa aattcctaag tatattctta
    60841 atgctattgc ttttgcatgt caggaaatag tagatctctc aatttataaa aaaattctac
    60901 tccatacaat cgacttgtac gaggaaacta cagaattgtc agctttatca gctagtttaa
    60961 aaacaagtga tacagctgat ttgaatatag caataatttc tttattagag tactttggta
    61021 aagatgagga tgttgtgtat ctaatcaatc agatagaaaa ttacacaaaa atactttatg
    61081 ctgaggtttc aaaatgacaa atatcttcga aaaaatcgta aatgatttaa atttagaaca
    61141 aaaagatgtt gtggaaagtg aagataacct ttatgtaaat gcatgtcctg ggagtgggaa
    61201 aacaagggta cttacaagga aaattgctta ccaagcaatt aaacacgatg gatcactaaa
    61261 aagaataatt gctatcacat acactaatag agctgctgaa gaaattaagg aacgcctaag
    61321 tttacttagt atagatgatg atattaatat ttgggtgggg acaatacatc agttttgtct
    61381 tgaatttatt atttatcctt ttaaaatgaa tctctcaaga gtatcaaaag gatttacaat
    61441 tattgatgat tatactcaaa gagcttacgt cagccaaatt aataatatgc tgaatttaaa
    61501 tctcaaaacg tatgaactga acaaaattga tttgtcactt tctcatgatt ttaaaattaa
    61561 tgaagttcag tatactcaag tttctgaaat gtatcacact atgttggaaa aaaataaaga
    61621 aattgatttt gatatgattt tactagtagc gtataaaatt ttaactgtaa acagtaacgt
    61681 tgcacgtaat attgctagta atataagggc gatttatgtg gatgaatttc aagatactag
    61741 agaattacaa tacaatgtaa tagctaaatt acttcaaaac aaccctcaaa ttcagtcaat
    61801 gtttgttgga gatattgacc aagcaattta tggatcatta gatggtatag ctaaatcagt
    61861 tgaagaacta gaaatgctaa ctagtcacac ttttcaatca aagacgctac atggctgtta
    61921 caggtctaat cagagattag tagacttcta ctctaatttt cagagttgtc cttacgagat
    61981 tgaaagcaga gggaataata aaaacgaccg tggttttata agttatgatt gcaaagttac
    62041 taaggaggat ttacccaaca taataaaaaa tataataaag gaaaagattg aaagcggcat
    62101 tcatgaaaaa gatatttgtg tacttgctcc tcaacactat cctctgtttt cactgggtga
    62161 gtatttaaag aaagaattac cgtattgcaa ttttgatgct ataaatatat caccaattaa
    62221 ggtaaataat catagtttat ttttcaagtt agcaattttg tactttactg aatcaggtga
    62281 aaaggttcga agaagaaagc taattgcaaa cgatatttta att
//
LOCUS       DAJSBL010000012        61355 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.012, whole genome shotgun sequence.
ACCESSION   DAJSBL010000012 DAJSBL010000000
VERSION     DAJSBL010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 61355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 61355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61355
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.012"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..563)
                     /locus_tag="ONT85_000860"
     CDS             complement(<1..563)
                     /locus_tag="ONT85_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HCR2377855.1"
                     /translation="METVRLWIFMKNFRSALRYVILESKSGYYLIDTDHYTFWGGYLF
                     PIFNWIIPHKVMKINLPAEEINRLILNKDLQRKREKEHSNVAWIGPAFAVVTSSIVGP
                     MLESIYSDFSIGIRYLFVTLSIAVLLCIKGYVSKKNKDILTLIGVNNLSSTKIILFPE
                     SIKEGSRLLIIFIMLILFSVLFGVMFI"
     gene            complement(750..1400)
                     /locus_tag="ONT85_000861"
     CDS             complement(750..1400)
                     /locus_tag="ONT85_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2377856.1"
                     /translation="MFIKGGQKMCFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLR
                     AGLITFISIVGFFLFTVIVFIWRTTAPPLPEITYGEFPFSLEYEIDEERILIEDTVIA
                     EFIGSFRGNVTTQPRRSWSIKLLNNSGELKAFHYEFLIIESEDVTITLSSGSAGYFMN
                     DGGSKPQLTVRDRIAGFMHFSHYDLEEARKILAEYGITLINWEYAPPIRNFFNDYY"
     gene            complement(1943..2878)
                     /gene="whiA"
                     /locus_tag="ONT85_000862"
     CDS             complement(1943..2878)
                     /gene="whiA"
                     /locus_tag="ONT85_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HCR2377857.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            complement(2912..3910)
                     /locus_tag="ONT85_000863"
     CDS             complement(2912..3910)
                     /locus_tag="ONT85_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HCR2377858.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAINR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            complement(3907..4791)
                     /gene="rapZ"
                     /locus_tag="ONT85_000864"
     CDS             complement(3907..4791)
                     /gene="rapZ"
                     /locus_tag="ONT85_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HCR2377859.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            complement(4926..5657)
                     /locus_tag="ONT85_000865"
     CDS             complement(4926..5657)
                     /locus_tag="ONT85_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HCR2377860.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLRPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTALQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSEALDTASEK"
     gene            complement(5661..6926)
                     /locus_tag="ONT85_000866"
     CDS             complement(5661..6926)
                     /locus_tag="ONT85_000866"
                     /EC_number="6.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HCR2377861.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            complement(7189..10005)
                     /gene="uvrA"
                     /locus_tag="ONT85_000867"
     CDS             complement(7189..10005)
                     /gene="uvrA"
                     /locus_tag="ONT85_000867"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HCR2377862.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQEIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            complement(10015..12009)
                     /gene="uvrB"
                     /locus_tag="ONT85_000868"
     CDS             complement(10015..12009)
                     /gene="uvrB"
                     /locus_tag="ONT85_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HCR2377863.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQIRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKDAAKALDFETAANVRDMILELKASK"
     gene            12268..13770
                     /locus_tag="ONT85_000869"
     CDS             12268..13770
                     /locus_tag="ONT85_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HCR2377864.1"
                     /translation="MKRKHLFHSFVFLMTGLVTFAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            13772..14509
                     /locus_tag="ONT85_000870"
     CDS             13772..14509
                     /locus_tag="ONT85_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HCR2377865.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(14554..14727)
                     /locus_tag="ONT85_000871"
     CDS             complement(14554..14727)
                     /locus_tag="ONT85_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2377866.1"
                     /translation="MEKKNQAQLSEGGIDYVLDIINEPMVLSYAMEQVERLLVCAGKQ
                     AMRLIRLGEKIRR"
     gene            14703..15368
                     /locus_tag="ONT85_000872"
     CDS             14703..15368
                     /locus_tag="ONT85_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR2377867.1"
                     /translation="MELDFSSPYYVVVGQLSDNQQLCQERINPLLELLERVFSYYGQA
                     LLTVHRQQIIILVNRISRASLLSLLDKIQTKFNKMYQLQLNFGIGSLCYTEQETPQSF
                     LHAKQVCEWIAFHQSVNEIRFFEDLDLGIVLPAIPSDQRTLYVKRILKSLTEEEVHLF
                     KKTLACFSKNNGSIKNCSEELFIHKNTLQYRLNKFHSLTGYTPRNYDDYHILKLAFLL
                     VQT"
     gene            complement(15450..16616)
                     /gene="dnaJ"
                     /locus_tag="ONT85_000873"
     CDS             complement(15450..16616)
                     /gene="dnaJ"
                     /locus_tag="ONT85_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HCR2377868.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(16767..18596)
                     /gene="dnaK"
                     /locus_tag="ONT85_000874"
     CDS             complement(16767..18596)
                     /gene="dnaK"
                     /locus_tag="ONT85_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HCR2377869.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            complement(18647..19210)
                     /gene="grpE"
                     /locus_tag="ONT85_000875"
     CDS             complement(18647..19210)
                     /gene="grpE"
                     /locus_tag="ONT85_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HCR2377870.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            complement(19304..20347)
                     /gene="hrcA"
                     /locus_tag="ONT85_000876"
     CDS             complement(19304..20347)
                     /gene="hrcA"
                     /locus_tag="ONT85_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HCR2377871.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEDKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            complement(20581..21747)
                     /locus_tag="ONT85_000877"
     CDS             complement(20581..21747)
                     /locus_tag="ONT85_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HCR2377872.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMKIMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYLDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            complement(21763..21990)
                     /locus_tag="ONT85_000878"
     CDS             complement(21763..21990)
                     /locus_tag="ONT85_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HCR2377873.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            22474..23064
                     /locus_tag="ONT85_000879"
     CDS             22474..23064
                     /locus_tag="ONT85_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HCR2377874.1"
                     /translation="MKTAITFLLEEYADWEGAYITSTLNQNEKWQNKTASIQSEVTSI
                     GGLKTKIDYLIEQIPEKVDLFIMIGGNSWDIKNHHLYQIIDKLLKTDVPVAAICGAVD
                     YLAQSGLLQGYKHTGNAQFPWLESENYQNKEDFIEQQAVKDRNLITANGTAPIEFTDL
                     ILQAVHFETEEIREKTIRLYKLGFYDYYKKYGDPFS"
     gene            23254..23967
                     /locus_tag="ONT85_000880"
     CDS             23254..23967
                     /locus_tag="ONT85_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2377875.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIRQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLIDVTVIDN"
     gene            complement(24082..25092)
                     /locus_tag="ONT85_000881"
     CDS             complement(24082..25092)
                     /locus_tag="ONT85_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HCR2377876.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPTWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQSSEKYQKVWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTIRHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(25129..25611)
                     /locus_tag="ONT85_000882"
     CDS             complement(25129..25611)
                     /locus_tag="ONT85_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA editing protein"
                     /protein_id="HCR2377877.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            25761..26129
                     /locus_tag="ONT85_000883"
     CDS             25761..26129
                     /locus_tag="ONT85_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HCR2377878.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            26594..27046
                     /locus_tag="ONT85_000884"
     CDS             26594..27046
                     /locus_tag="ONT85_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR2377879.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQFREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNYVVNFSQWINEKLLIQTDISLVS"
     gene            complement(27170..28021)
                     /locus_tag="ONT85_000885"
     CDS             complement(27170..28021)
                     /locus_tag="ONT85_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HCR2377880.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(28034..28819)
                     /locus_tag="ONT85_000886"
     CDS             complement(28034..28819)
                     /locus_tag="ONT85_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR2377881.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWVNNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENNKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            complement(29171..30112)
                     /gene="ribF"
                     /locus_tag="ONT85_000887"
     CDS             complement(29171..30112)
                     /gene="ribF"
                     /locus_tag="ONT85_000887"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HCR2377882.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGETWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            complement(30116..31039)
                     /gene="truB"
                     /locus_tag="ONT85_000888"
     CDS             complement(30116..31039)
                     /gene="truB"
                     /locus_tag="ONT85_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HCR2377883.1"
                     /translation="MDGLLPLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEKKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVERFKRVDITDEVWQKVKNGMRLDYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            complement(31407..33769)
                     /locus_tag="ONT85_000889"
                     /pseudo
     CDS             complement(31407..33769)
                     /locus_tag="ONT85_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
     gene            34217..34390
                     /locus_tag="ONT85_000890"
     CDS             34217..34390
                     /locus_tag="ONT85_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCR2377884.1"
                     /translation="MIFLLTVNGPTNSNFYGAGSTDLLVTWLYLTVTKIDYNLASVIG
                     ILIFIYMQKFIYY"
     gene            complement(34547..34894)
                     /gene="rbfA"
                     /locus_tag="ONT85_000891"
     CDS             complement(34547..34894)
                     /gene="rbfA"
                     /locus_tag="ONT85_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HCR2377885.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKTAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(34921..37227)
                     /gene="infB"
                     /locus_tag="ONT85_000892"
     CDS             complement(34921..37227)
                     /gene="infB"
                     /locus_tag="ONT85_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HCR2377886.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSNDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            complement(37240..37551)
                     /locus_tag="ONT85_000893"
     CDS             complement(37240..37551)
                     /locus_tag="ONT85_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HCR2377887.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            complement(37548..37841)
                     /locus_tag="ONT85_000894"
     CDS             complement(37548..37841)
                     /locus_tag="ONT85_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HCR2377888.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            complement(37863..39038)
                     /gene="nusA"
                     /locus_tag="ONT85_000895"
     CDS             complement(37863..39038)
                     /gene="nusA"
                     /locus_tag="ONT85_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HCR2377889.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            complement(39061..39534)
                     /gene="rimP"
                     /locus_tag="ONT85_000896"
     CDS             complement(39061..39534)
                     /gene="rimP"
                     /locus_tag="ONT85_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HCR2377890.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            complement(39679..44037)
                     /locus_tag="ONT85_000897"
     CDS             complement(39679..44037)
                     /locus_tag="ONT85_000897"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HCR2377891.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIH
                     IQLGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSF
                     IVKKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYA
                     PEGEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGK
                     KAGVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAV
                     KMYKGNVIESFDEFIDPGHPLSRTTVDLTGITDGMVRGSKSEEEVLRMFLEFSKDTIL
                     VAHNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSL
                     EQHHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILA
                     KNQAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKG
                     VEEARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVAT
                     GNVHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEI
                     VVENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRL
                     KKELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAP
                     HYYCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPD
                     IDLNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSA
                     EVDRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHS
                     IHDNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIY
                     SKTGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDAT
                     LAEVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVP
                     DWYIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMC
                     KGKDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFV
                     IEGDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGV
                     LKDLPDENQLSLFDML"
     gene            complement(44249..45958)
                     /locus_tag="ONT85_000898"
     CDS             complement(44249..45958)
                     /locus_tag="ONT85_000898"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HCR2377892.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPERDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTNLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELVNTLPILLN
                     QGE"
     gene            complement(46026..47294)
                     /gene="rseP"
                     /locus_tag="ONT85_000899"
     CDS             complement(46026..47294)
                     /gene="rseP"
                     /locus_tag="ONT85_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HCR2377893.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAILLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPDKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            complement(47455..48255)
                     /locus_tag="ONT85_000900"
     CDS             complement(47455..48255)
                     /locus_tag="ONT85_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HCR2377894.1"
                     /translation="MKQRVVTAAVALVLFIPIIWIGGIAVELTAAILAVIGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            complement(48252..49064)
                     /locus_tag="ONT85_000901"
     CDS             complement(48252..49064)
                     /locus_tag="ONT85_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HCR2377895.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            complement(49259..49816)
                     /gene="frr"
                     /locus_tag="ONT85_000902"
     CDS             complement(49259..49816)
                     /gene="frr"
                     /locus_tag="ONT85_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HCR2377896.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(49819..50541)
                     /locus_tag="ONT85_000903"
     CDS             complement(49819..50541)
                     /locus_tag="ONT85_000903"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HCR2377897.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(50677..51558)
                     /locus_tag="ONT85_000904"
     CDS             complement(50677..51558)
                     /locus_tag="ONT85_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HCR2377898.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(51657..52439)
                     /gene="rpsB"
                     /locus_tag="ONT85_000905"
     CDS             complement(51657..52439)
                     /gene="rpsB"
                     /locus_tag="ONT85_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HCR2377899.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            52798..53277
                     /locus_tag="ONT85_000906"
     CDS             52798..53277
                     /locus_tag="ONT85_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HCR2377900.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            53494..54585
                     /gene="phnW"
                     /locus_tag="ONT85_000907"
     CDS             53494..54585
                     /gene="phnW"
                     /locus_tag="ONT85_000907"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HCR2377901.1"
                     /translation="MYKLLTSGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSSSFGVESVLSSVIRPEDTVLICSNGAYRQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQERDLL
                     FIVDAMSSFGGIPICVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            54578..54706
                     /locus_tag="ONT85_000908"
     CDS             54578..54706
                     /locus_tag="ONT85_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HCR2377902.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIR"
     gene            54710..55255
                     /locus_tag="ONT85_000909"
     CDS             54710..55255
                     /locus_tag="ONT85_000909"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HCR2377903.1"
                     /translation="MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEH
                     YLFKDLVKHSTLKPDTLETIERLRNAGINIGSTNGFNAPMMTIVAETAKKERYSPDFM
                     ATFEDVEGYGRPFPYMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEG
                     SSLMGISYDEYQKLEMGKKIV"
     gene            complement(55709..57400)
                     /locus_tag="ONT85_000910"
     CDS             complement(55709..57400)
                     /locus_tag="ONT85_000910"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HCR2377904.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDKEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMHHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(57820..58767)
                     /gene="arcC"
                     /locus_tag="ONT85_000911"
     CDS             complement(57820..58767)
                     /gene="arcC"
                     /locus_tag="ONT85_000911"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HCR2377905.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(58882..59901)
                     /gene="argF"
                     /locus_tag="ONT85_000912"
     CDS             complement(58882..59901)
                     /gene="argF"
                     /locus_tag="ONT85_000912"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HCR2377906.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(59992..61221)
                     /gene="arcA"
                     /locus_tag="ONT85_000913"
     CDS             complement(59992..61221)
                     /gene="arcA"
                     /locus_tag="ONT85_000913"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HCR2377907.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
BASE COUNT        17055 a        13102 c        10622 g        20576 t
ORIGIN      
        1 tctataaaca tcaccccaaa taaaacagaa aacagtataa gcattataaa aattatcaac
       61 aaccttgatc cttcttttat agattcagga aacagtataa ttttagtact agataaattg
      121 tttacaccga ttagtgttaa aatatcttta ttcttcttgc taacataacc ctttatgcac
      181 aaaagaacag ctatacttaa agtaacaaat aaataacgta ttccaataga aaaatcacta
      241 taaatactct ctagcatagg accgacaatt gaagaagtca ctactgcaaa tgctggacct
      301 atccaagcaa cattagaatg ttctttctct cttttccttt gtaaatcttt gttgagtatt
      361 aatctattta tttcttctgc aggcaagtta attttcataa ccttatgggg gattatccaa
      421 ttaaaaatcg gaaaaaggta gcctccccaa aaagtatagt gatctgtgtc aatcagataa
      481 taacctgatt ttgattccag aataacatat ctcaaagccg aacgaaaatt tttcatgaat
      541 atccataatc tcactgtttc cacacctaat tcctccaatt ccaattaaat ggatttatag
      601 catcaccaat tatgttaact gcctgaccaa cctcattgat aaaattggca ccactatccc
      661 atgcttcaac aatgctatat atctatcaaa caaaaaatgg aaaactaaat actcgcgcac
      721 ataaatttta cattctgttt tttataagac taataataat cattgaaaaa atttcttatc
      781 ggtggcgcat attcccaatt gattaatgtg atcccatatt ctgcaagtat tttacgagct
      841 tcttctaagt catagtgaga aaaatgcata aaacctgcaa tacgatcacg gactgtgagc
      901 tgtggcttgc ttccaccatc attcataaaa tatccagcag agccactact taaagtgatt
      961 gttacatctt cactttctat tattaagaat tcataatgaa atgctttcaa ctctccacta
     1021 ttatttaaaa gttttatact ccaacttcgt ctaggttgag ttgtcacatt ccctctaaaa
     1081 cttcctataa attctgcaat caccgtatct tcaatcaaaa ttctttcctc atctatttca
     1141 tattcaagac taaacggaaa ttctccatat gtaatctctg gcaatggtgg tgctgtagtt
     1201 ctccaaataa atacaataac tgtgaataga aaaaatccaa cgatcgatat aaatgttatg
     1261 agacctgctc gaagtaaaac ccgatataat aaaaatgctg tatttcgctt tgctatatct
     1321 agaaaaataa aaagtactgc aatcataatg atagtaaaag ttgtaaaaaa gcacattttt
     1381 tgccctccct ttataaacat cgattctcaa attctaaacc aaatcttgat agttcaagtt
     1441 caggtataac gtataccaac catacatccc aattactatt tatgaactta tgattatcac
     1501 tgaataaatc actccatccc cttgttaaaa cttctacatt aattacacct ggttgttttc
     1561 gactagctaa tccaatgaac ttatcaacca tccttatcca ccaaccacga gaatcttgac
     1621 cagaagtaag aaaaacagtt ctcaaggttg atcgagcagt tccttccagt ttttaattaa
     1681 tctccagtta tagataaata cttgatccta tctatatcag gacttctata ttaagcaaaa
     1741 gtttatgtat aaaactaaaa tcttcattca gttgtctccc cgctttttat acgattgtat
     1801 taaaaaacaa tagaagcctt tcaacattat gttactccag taaagttgga attatagttt
     1861 gacggaagaa aagcaagtat agcttttaaa aaaaactgat acattcgcat ctcctgcttc
     1921 atgtatcagt tttttctttg gattaaactg cttcttcacg cagtttttcc gcaaattcat
     1981 ttattttgcg aattcggtga ttaattcccg attttgatat tgcacctgag ggaatcattt
     2041 cccctaattc tttcaagctg acctctggat gttgaagccg aagttctgcg atttcctgaa
     2101 gtttttcagg aagggcatgt agaccgacct ttgcttcgat caattcgatg ttttcgattt
     2161 gtttagaagc cgcatcaatc gtcttgttca gatttgctgt ctcacaattg actagacgat
     2221 tgaccgaatt tctcatatcc cgtacgattc gaacatcttc aaatcggagc attgagttgg
     2281 ttgcaccaat caatgttaag aaatcagcaa ttttttctgc tccttttaaa tacgaaatat
     2341 agccattgcg ccgttcaagc gttcgagcat gtagaccgta ataatttaac atatcgcaaa
     2401 tgtcttggtt atgctgttca taaatagaaa atatttccaa atgataacga ctcgtttccg
     2461 gattattgac tgaacctgag gccataaaag caccacgtaa gtaagaacgc attttttgcg
     2521 aatttcccat aatctcgtcc gaaacgtgtc cattgaacat cataccatcc atgatatcaa
     2581 gatccatcaa cacatctttt gtgccttgct tcaaccggac gatatagaca ttgttctttt
     2641 ttaatttcat ttttttacga acgagtagct cacttcgtac ctgataatga tctttcaaaa
     2701 gagaatagat acgtctggca attgcagcat tttctgtttg gacgttcaaa ataaactgct
     2761 ggttcacaat acctaaagag ccgttcattc gcagcaaagc agctaattcc gctttcgcat
     2821 gttctcgatg aacttccaaa ccagttagtt ctttcttcac atctgcagca aatgacatcc
     2881 tattctcctc ctttctccaa caataaattg cttaatattt tgaaccgaaa actaaacgga
     2941 acaattcttc tactactttg tctccatcgt ggaagacacc accatctttc aattctaaaa
     3001 agtcggtgga aatcactcga catccttcat ttctcaatcc gttgaagtcg tgtgcgactt
     3061 gtactagata ctcatcatag atctctgggt ccatataatc ttcaggcact ttttccgtat
     3121 tgaccaatac tgtatcgata aaaggctgat tcaaatgctt gtgtaaaaca cgtatatgat
     3181 cggcatctgt gaaatgttcc gtttcccctt tttgggtcat gatattacag atatagacgg
     3241 tttcagcagt tgtcttgagc attgcttcgc cgatttctgg tatcactaaa tttggaagga
     3301 tactggtgaa taaacttccg ggtcccaaaa cgaccatgtc ggcgtccata attgctgaaa
     3361 cgacttttcg agccgctttg gcttcacttt tatcatttgt gttgcagaca tacacacggt
     3421 caatggtttt acggttgatc gcgattttag attctcctac tgcagtcgtt ccgtctttaa
     3481 aaacagcatg caaggtcaaa ggagtttctg aagaaggata aacatgtcca tctacatgca
     3541 tcatttttgc taataattga atcgcctcat aagtgcttcc ccgcatctcg gaaactgcgg
     3601 caatgatcag attaccgatc gtatgattgg ctaaaaactg atcttccttt ttgaaacggt
     3661 attggaaaat atcttcatag aattgtggca tatccgataa cgccactaag acatttctca
     3721 agtcaccagg aggagccata gcgatcgatt ctctgattgc accgctgctt ccaccatcat
     3781 ctgcaacagt cactactgct gtgatatctg ctccttgatt tcgtaaactt ttcaggataa
     3841 ctggcagacc agtgccaccg ccgaccacta cgatctttgg ttttcttatc cggtaggttt
     3901 tcattttcac gaccggttca ccgtctcttt ccttttatcc ttgtcccgat gtgtcacatt
     3961 gactttatag tttttcccta aagcatttgc caaacgttca gtcaatgcca cagaacggtg
     4021 ttgaccgcct gtacagccga ttgcaactgt caagctgctt tttccttctt taatgtatcc
     4081 aggcaataca gtttccagaa gatccaaaaa tttatggtag aatgattgcg tttcatcaaa
     4141 ggacatcaca taatcataga ctggctgatc ttttccggtc aatggtctta gttccgggat
     4201 ataatgaggg ttgggcaaaa aacgcacatc catcacaata tccgaatcga tcggcaaccc
     4261 gtatttaaaa ccaaatgata caaattcgat ccgaaatcct tggtctccct ttgatttgaa
     4321 tgactcattg attttttcac gtaattgtcg tggtgtcaat gttgtcgtat caatgatcac
     4381 ggaagcttgg gctttcaagt cgtctagtat cgcccgttct ttacgtatcc cttcagtgat
     4441 caaaccgtcc atcgccattg gatgtgctcg tcttgtttct ttgtagcgag ccactagttc
     4501 ttcgtctgaa gcatctaaaa ataagataga agtgtcgatg aagttcgtat tttcgatttc
     4561 gatcagcatg ttttggattt cttcaaagaa ggaacgagaa cgcaaatcca ctcctaaagc
     4621 catttttgtg actttacctg attctttgat caattcccag aattttggaa tcaatcgagg
     4681 cggcatatta tcgatacaaa aatagcccat atcttcaaaa ctttggatag ctactgtttt
     4741 ccctgcacca ctcatacctg ttatgatgac taattgcaaa ttatctgtca tatttacgaa
     4801 cccctctcac attgtgcata ataaaattat atcatcccgg gcgtatcatt accagttttc
     4861 tttttctttt acaaaaagat ttggaccaaa tatcttttag gatatttgcc ccaaatccaa
     4921 tttttttact tttccgatgc tgtatccaat gcctcgcttc tttcagctaa tgcacgttcg
     4981 atcgttcgat ccgctaaaat agattgagca tcgatcactg ggtaatgatt atcttcagca
     5041 aattcatgca tcaaagataa ttctgtgcaa cctaggatga ctttttcaca gtttaggcgc
     5101 tctaccgctt cctctaagat ttcgtaatag agctcttgat tcaaataatc cgattccttg
     5161 atctcatgat agatcaggta attgattttt tcttgaagcg cagtatcagg aatcactgtt
     5221 tcgaaaccaa gatttttgac ttcgcgttca tagattccag ctttcatact tccttctgtt
     5281 cctagaatcg ctactctccc tgtcgtatgc tgacggacaa gttcatttgc tgcttctctt
     5341 ggcatatgca agataggaat atccgttgcc gcttgtaact cttcaaaaaa gtagtgtgcg
     5401 gtgttacaag tcaacacaat gaagtttggt cgcaataaat tttgtttctc aatatcatcc
     5461 agcagaaacg gcattggatt ttcttctgat cgatctaaaa tataagctgt acgatcagga
     5521 actgttgcat gattgaataa gacataattc aaatattctt gatcttttgt agctttcgtt
     5581 cggtggttga tcaagcgaac aaaactttct gtcgccatcg tgcccattcc gcctaaaata
     5641 ctgaaaaaat tctccatgaa ctagccttct ttcacatgaa aatacttttt gaatcttgga
     5701 atatagttat ggaacatata tttcatcaac aaccagcgct tgattgatcg gtctttttca
     5761 taaaatacag tagtaccgta acgattttct ttgatcattt gctcagctag ttttttgtct
     5821 tcattttccc gagcatattc taaaaagatt tttttgggca cacccatcca taatcttgct
     5881 ttatctgatt gattcgttcc ataggtcgtt tcaacaaagg gtttgttgaa cactcgatct
     5941 tctgtgacaa aacgagccag attcaagccg ttcaatgtca caaagaaact gctacgacct
     6001 tgtctcaaat tgatttcaaa taatttatat tctccatctc taggatcata cttcatatcg
     6061 aaattggcaa agcctgtata ttcgattttt tccaaaaatg ccttaatggt ttgatagatt
     6121 ttttcgttat aatctggcat gatcacaacg taattaccaa tcgatgctgg tgtaggatct
     6181 tctaatagtg gatgaccaag gcacatcatc cgaacttgat gatcttcatc tacatacgca
     6241 tttagcacac gcatattact atcatcaccc gggataaaat cttgtacaat catttcactt
     6301 ttataaccgg cttcgtatat ccggcctaaa attaaatcga actcttctct agtctctaaa
     6361 ataaaagctt tttttcttcc ttcaaattgg acagaaagat actccacact gtttgcaggt
     6421 ttcaaagcaa ctgggaattc aaaaggcaat tcctgttcca aatgtccatt tactaacatt
     6481 tcttctctga cgatcaatgt tttaggatac ggaagatcat attcttcaca aacttcataa
     6541 aagcttactt tattgatcag cctttcaaat aaagaataat caatatatgg acagataaaa
     6601 tattctgaca gctcttgttt atgttgggag atcaattctg catatccatc accacaagca
     6661 atcaaaagat attttttact cttatcagaa tatctttctt ttgccaaacg aagcatcgtc
     6721 tcgataaata cagggtcttt atcaaatcct ggaataactt ctacattgac aattttactg
     6781 tagcgggtag gggctaactg atccgaagca tatgcttggc agattttgcc gtatgcttca
     6841 ttaaaagaac gtgccatacc ataaacgttc atgtcgcttc ctaataatat agggataaat
     6901 tcttcatttt caatactgtt catcattatc tctcacttac tcataatctc ttgtttttct
     6961 tttccaacta cacaaggaat atcataccat aaagaaaaaa gcaatcaaag gaaagtcctc
     7021 cgattgctca attctttttt gaacggattg aacttatagg aagtttttat ctataccaat
     7081 cagtgtagaa agaactacca tgtaatgatt ctagaacact gatattaata aaacaagcaa
     7141 aaattcattt ttttatgttt ttttaactat ctcagctatc tatcctcttt ataccattat
     7201 ttttttcaaa tagtgacctg tataactttc ttttacgtta atgatttctt ctggtgttcc
     7261 agtcgcaagg atcgttcctc caccttctcc accttctggt cctaaatcaa tgatataatc
     7321 tgctgttttg attacatcta gattgtgttc aatcactaaa actgtgttac cagcatctac
     7381 taatctttgt aatacatgca acaagcgggc gatgtcatcg ctatgaagtc ctgtcgttgg
     7441 ttcatctagt atatagaaat tctttccatt agagattttg tgcaattcac tggcaagttt
     7501 catccgttgt gcctcaccac cggacaatgt cgttgctggt tgccccatcg tcacataacc
     7561 taagccaaca tcaacaatcg tttgcagttt gcgatgaatc tttggaatgt gcttgaagaa
     7621 ttctacagca tcctctactg tcatttccaa aatatcagca atgctttttc ctttgtaatg
     7681 cacttctaat gtttcagaat tatatcgttt accatgacat acttcacaag gaacatagac
     7741 atcaggcaaa aagtgcattt cgatcttaat aattccatcc ccgcgacaag cttcacaacg
     7801 accgcctttt acgttgaaac taaaacgccc tttcttataa ccccgcattt ttgcctcgtt
     7861 cgtttgagca aataacccac ggatatcatc aaatacactt gtataagtcg ctggattact
     7921 tctcggcgtc cggccgattg ggctttgatc gatatcgatg atcttttcga tactttcgta
     7981 gccggaaatt gtcttgaatt tacctggctt agcagaattc ttatttaatt tttgcgctaa
     8041 cgatttcttt aagatactat tgaccaatgt actctttcca gaaccagaca cacctgtgac
     8101 tgctacaaat tctcctaatg gaaattcaac actaatattt ttcagattgt tttctgaagc
     8161 accagtgatc ttgattgctt tcccattacc tttacggcgt tcttttggta cagggattga
     8221 ttttttccct gaaagatatt tccctgtcaa agaattttca tcattagcta cttcttcagg
     8281 agtgcctgca gccacgattt gtccgccttg ttctccggcc ccaggtccga cgtcaatcaa
     8341 ataatctgct gcccacatcg tatcttcatc atgttcgact acaacgaggg tgttgccaag
     8401 atcacgcatt ttctttaatg agccaatcag tcgatcgttg tcgcgttgat gcaatccgat
     8461 cgatggttca tctaaaatat acaaaactcc agaaagattc gatccaattt gtgtagccaa
     8521 tctgattcgc tgcgcttctc ccccagataa tgttccggaa gcgcgactca aagttagata
     8581 gtctagtccc acattttcca agaatgaaag tctgtcattt acttctttta aaatcggttt
     8641 ggcaattact ttctcttgtt cagataacgt taagtttgag aagaaatcta ctgctttttg
     8701 aatagacaac tcatttgttt gcccgatatt cgttccattg atttgaacag atagagcttg
     8761 gggattcaat cgataacctt tacatgtctg acaagttaat tctgtcatat aaagacgcat
     8821 ttgctcacgc gtgaaatcac tgttcgtatc atgataacga cgctcgatat tcttcaatac
     8881 cccttcgaaa gtcgtttcta catcgcgaac gccaccaaaa tcattttcat aatggaaatg
     8941 gaaaagatca tctgtcccat tcaagatgat ctcctgatgc tcttccggta attcttcgaa
     9001 tggtgtatct aaatcgatac cgaaactttc acaagcctgc gctaacattt gaggataata
     9061 ctgagagctg attggattcc atggaacgat agcaccttga ctaagtgtct tcgttctatc
     9121 tggaatcact aaatcttgat cgacttctaa tttgacacct aaaccatcac agtctggaca
     9181 agctccaaaa ggcgcattga atgagaacag tcttggttct agttcgccaa cagtaaatcc
     9241 gcaataagga caggcatagt gttcgctaaa caacatttct tcttcaccga tcacgtcaac
     9301 cagagcatag ccgtctgcta atcgcagggc cgcctctaaa gaatcaaata ggcgagaacg
     9361 aatcccttct ttcacaatga tccggtcgat gacgatcgcg atatcatgtt ttttgttttt
     9421 ctccagctca ggcacttcac ttaaatcata gatctcacca tcgactcgaa cgcggacgta
     9481 tccctctttt tggattcgtt cgaatatttt tttatgttgt ccttttttct ttaccacgat
     9541 cggtgccaac acttggatct ttgtgcgctc aggcaaagcc attacttgat ctgccatttg
     9601 atctactgat tgactggtaa tctcgatatg atcgttggga caaatcggat gtcctacacg
     9661 tgcaaacaac aagcgtaaat agtcattgat ttccgtcaca gtacctactg tcgaccgagg
     9721 atttttactt gttgttttct gatcaataga aatcgccgga ctcaatccgt cgatgctatc
     9781 tacgtccggc ttatccattt gtcctaaaaa ctgacgagca taagcagaaa ggctctctac
     9841 gtatctccgc tgcccttcag cgtacagcgt atcaaatgct agggaacttt ttccagaacc
     9901 ggacaaacct gtaacgacca cgaatttatc tcgtggaatc gttacatcta tatttttcaa
     9961 gttatgggca cgtgcaccat gaatcgtaat tttatcgtta gccatgttgt cctcctactt
    10021 tgaagctttt aattctaaaa tcatatctcg cacattcgcg gctgtttcaa aatccaaagc
    10081 ttttgccgca tctttcattt ctttttctaa tttcatcaat aacgtgtctt tttcttcctt
    10141 cgtcatttct tcgtacgaga cttgtaccgc ttcttttgta tcatcttccg attctttcgt
    10201 aatagaaatc aaatctcgga tttctttaat gatcgttttt ggaacgatcc catgttcttc
    10261 attgtacttc tgctgaatcg tacgacgtct tgacgtttca tccatcgcta gacgcataga
    10321 atctgttact ttgtctgcgt acatgattac tttaccttct tcattccgag cagctcgtcc
    10381 aatcgtttgg actaaggaac gttcactacg taaaaagcct tctttatccg catccaagat
    10441 agcaacaagg gaaacttcag gtacgtctaa tccttcacga agcaaattga ttccaactaa
    10501 cacatcaaat tcacctaatc gaagatctcg aatgatttct gttcgttcca gcgttttgat
    10561 atcactatgc aaatatttga ctttgatccc taattctttg aaataatctg tgaggtcttc
    10621 tgccattttt ttcgtcaatg tcgttacaaa cacccgctgg tctttttcga ctcgttcatt
    10681 tatttcgcct actagatcat cgatttgtcc catgattgga cgaacttcga tcactgggtc
    10741 aagcagccct gtcggacgga tgatttgttg gataacggta tctgtttgtt catgttcata
    10801 tggtcctggc gtcgcagaga cataaatgat ctgattgaca tgtttttcaa actcttctaa
    10861 ccgaagtggc cgattgtcta aagcagatgg caaacggaaa ccgtaatcta ctagcatctg
    10921 ttttctcgct cggtcaccat tatacattcc tctgatttga ggcatggtta cgtgcgattc
    10981 gtccgccacg atcaaaaaat cttcaggaaa gaaatccaat agcgtataag gcggttcacc
    11041 ttctttacgt ccgtccatat gccgagaata attttcaatt cccgacgtgt agcccatttc
    11101 taacatcatt tcaatatcgt aattggttcg ctgttctaaa cgttgagctt ccagaagttt
    11161 gttttcattt cgcaagacag ttaaacgctg ttctaattcc gctttgatat tcgctacggc
    11221 atgctccata tgttcatcat tcgtcacaaa gtgagtcgcc gggaagattg ctacgtgttc
    11281 cgtttctcct aaaacttcac ctgtcagtgc attgacttct cggatacgtt cgacctcatc
    11341 tccaaaaaac tccacacgta aggcacgttc atctcttgaa gccggaaaaa tctccacgac
    11401 atcaccgcgc actcgaaaac gaccgcgctg aaaatctata tcatttcttt cgaattggat
    11461 actgactaag tcacgaatca actgattgcg atctagttct gctccctgtc gaatcgaaac
    11521 tacttgtttt tggtactcaa aaggcgagcc taatccaaaa atacatgaaa cggatgcaat
    11581 gacgatcaca tcatttcttt ctaataatga actagtagcc gaatgtcgta atttgtcgat
    11641 ctcatcattt acacttgaat ctttttcgat atacgtatcg cttgaaggca cataagcttc
    11701 aggctgataa taatcataat aagaaacaaa atattcaaca gcattgttag ggaaaaattc
    11761 cttgaattcc ccgtacagct gaccagccaa tgttttgtta tgggcaataa tcagtgttgg
    11821 tttattcact ttctcaataa gattggaaat cgtatatgtt ttccctgttc cagtcgcacc
    11881 taataagatt tgtgcttttt tcccgccgac tacgccatcc actaattggt taatcgcttc
    11941 aggctgatca ccagccggtt ggtatttcga gacaagctca aagtgtcggg aggtatctct
    12001 ctcaatcatt cttcgtcctc cttttcattc attccttttc attttacgac caataaaaag
    12061 aaaaggaaag caaaggcatt ccttactaaa aaattatacc acaccgaaca tactttcgcc
    12121 acttttttat cttattatac atgttatgtt ttataacata aaatgttaga aactcgcatt
    12181 tttttcattt tttcgagctt acagtcttgt ctgattcagc acttttcgcc tataataata
    12241 aaatattgga aatttaggag gttgattatg aaaagaaagc atttatttca ctcatttgtt
    12301 ttcttaatga caggacttgt gacatttgcg tttggtcatg ttgcacaagc tgacaacact
    12361 acaacggcag agtcaagcac aagcgagaca atgcaaaaaa tagagcccaa aaaagacgta
    12421 tacgtaattg ccagtgactc tgcttttgct ccatttgaat ttcaaaacgc aaagggcgag
    12481 tacgaaggca tcgatgtaaa attgatggaa aacatcgcca aactgcaagg atttaacatc
    12541 cgaatggact accgtggttt ctctgctgct ttgcaagctt tggaatcagg acaagcagac
    12601 ggaatgattg ccggaatgac tgtaacagat gaacgaaaga aaagctatga tttttctgat
    12661 tcttactttg atagcgggat ccaattagct gtcaaaaaag gagatacttc gatcaagtct
    12721 tatgaagatt tgaaaggaaa aacagtcgga gcaaaagttg gtaccgaaag tgctgaattt
    12781 ttaagcgacc atgaatctga atatggctat tctatcaaat attttgatgc tgctgatcaa
    12841 ttgtacgacg ggttaaaagt tggcgcaatc gatgcaatga tggatgatta tccggtaatc
    12901 ggttatgcga tcaaacaagg acaagaattg gaaacaccga tcaaaaaaga atctggcgga
    12961 aaatatgcat ttgccgttaa aaaaggacaa aatcctgagc ttcttaatat gttcaatgaa
    13021 ggattgaaag aattaaaaag aacaggcgaa tacgataaga ttattaaaga atatgtagcg
    13081 gatggttcag aaacttccag tgagtcttcc actgctgatg aatcaacatt tcttggactt
    13141 atccaaaaca actatcaagc attgttgaaa ggactttgga gaacaattct cctaactttg
    13201 atctcctttg ctttggctat agtaatcggt gtgatttttg gattattcag tgtagcacca
    13261 atacgcgctc ttcgcacaat tgctagtatt tatgtcgata tcatccgagg aattccaatg
    13321 atggttcttg cgttctttat tttctttggg ttgcctggta tcgttggatt tacgattcct
    13381 gacttccttg ctggtgttat caccttgact cttaacgcaa gtgcttatat cgcagagatt
    13441 gttcgaggcg ggatcaacgc cgttcctgtc ggacaaatgg aagcttccag aagtttaggt
    13501 ctttcttata atcggacaat gcaaaaaatc attttgccgc aagcgatacg catcatgatc
    13561 ccatcattcg tcaatcagtt cgttatctca ttaaaagaca caacgatcat ttccgcaatc
    13621 ggtgtagtcg aattgctgca aactggtaag atcattgttg cacgtacaac acaaagtacg
    13681 tatgtttact tgatcattgc gatcatgtac ttgattttga tcacagcatt gacaaaatta
    13741 gcaaaagttt tagaaaagaa ggtgaagtaa gatggcagaa aaaatcttag ttgagcattt
    13801 agtcaaaaaa tacggcgata acacagtatt aaacgacatc aatgtatcca tcaacgaagg
    13861 agatgtcgtc tgtatcattg gcccttcagg ttcaggaaaa agtacattct tgcgttgctt
    13921 gaatcgttta gaagaacctt ctgacggtga aattattatt gacggcgccc acttgatgga
    13981 taaaagcaca gatatcaact tggtgagaca acatatcgga atggtattcc aacactttaa
    14041 cctattccct catctttcta ttttggaaaa catcatttta gcaccgatgg acctgaagaa
    14101 agaatctaga gaagaagcag agaaaaaagc aatcaaatta ctgcagacag ttggtctaga
    14161 agataaaaaa gatatgtatc ctgaaatgct ttctggtgga caaaaacagc gtgtggctat
    14221 tgctcgtgct ctagcgatga accctgacat catgcttttt gatgagccta cttctgcact
    14281 cgatcctgaa atggtcggtg atgtcttgaa cgtcatgaaa aagttagcag aacaaggtat
    14341 gacgatggtt atcgttacgc acgaaatggg gttcgctaaa gaagtggcca atcgcgtcat
    14401 gttcattgac gacggaaact ttttagaaga tggaacgcct caacaaatct ttgaaaatcc
    14461 gcaaaatgaa cggacaaaag atttcttgaa taaagtatta aatatttaat aaaaaccaaa
    14521 aatgcacggt cgccatgaag ggaccgtgta tttttatctt cttattttct cacctaatcg
    14581 tatcagccgc atcgcttgtt ttcctgcaca aacaagcaat cgctctacct gttccatcgc
    14641 ataagaaagt accatcggtt cattgatgat atcaagtaca tagtcgatac caccctcact
    14701 taattgagct tgatttttct tctccatatt atgtagttgt gggacaactt tctgacaatc
    14761 agcaattgtg tcaagaacgg atcaatcctt tattggagtt gctcgaaagg gtcttctctt
    14821 attacggaca ggctttgctc actgtacata gacaacaaat cattatccta gtcaatcgga
    14881 tttccagagc ctcattgcta tcattgcttg ataaaatcca gacaaagttt aataaaatgt
    14941 atcagttaca gctaaacttt ggaattgggt cgctttgtta tacagagcaa gagacaccgc
    15001 aatctttcct tcatgccaaa caagtttgcg aatggatagc tttccatcaa tctgtgaacg
    15061 agatccgctt ttttgaagat ttagacctcg ggatcgtttt accagcaatc ccgtcagatc
    15121 aacgaacatt atatgtaaaa cgtattttga aaagcctgac agaagaagaa gtacatttgt
    15181 tcaagaagac tttggcatgc ttcagtaaaa ataacggaag catcaaaaat tgctcagaag
    15241 aattatttat tcataaaaac accttgcaat atcgattgaa caagttccat tctctcactg
    15301 gttatacccc tagaaactat gacgattatc atattctaaa gctggctttt ttgttggtac
    15361 aaacatgaca actacaaaaa aaccaggcga acatatgatt gttcgtctgg tttttaatta
    15421 ttaatgactt agcgttttta ctagaaaaac tacttccgct ttttcccgcc aaaagcatct
    15481 ttaaacttat cgaagaaacc gtcttcttgc tgttcttcaa ctttgatgcc gccagcttca
    15541 gcgaatgcac gtaatgcttc tctttgttgt tcatttagat ttttcggtgt gatcagtttt
    15601 actttgacat gttggtcgcc tgttgcacca ccacgtaact tcggagcgcc ttttccacgt
    15661 aaacggaagt tcgtacctgt ctgtgttcca gcagggatct tcaatttcac atctccatgg
    15721 acagttggta cttggacttc atcccccaaa gctgcttgta caaaacttaa aggcaattca
    15781 tagtaaattt cagaaccttc acgatcaaag atatcacttt cttctacacg gaagaccaca
    15841 tacaaatcac caaatggtcc gccgttttct ccagcttctc cttgaccagc taaacgcatt
    15901 tgttgtcctt cttctacacc agctggtaca ttcactttaa ctgaatgtgc tttcttctca
    15961 tgtcctgagc catgacaggt tggacaaggt tctttgattt ctttccctgt cccatgacaa
    16021 acatcacaag tttgacggct catcactcgt ccaagtggtg tttggcgttc tacattaatc
    16081 gtacctgcac catgacattt atgacaagta gttggttgtg tgcctggttt tgcaccgttc
    16141 ccaccacaag tatgacagat ttcttcccga ttatacttga tttctttttc tacaccaaaa
    16201 atcgcttctt caaaagacaa gtcgaccgta tattgcaaat cagcaccttg acgaggcgca
    16261 tttggatcga ctgaccgacc gcctcctcca aagaaagagt cgaagatatc ttcaaaacca
    16321 ccgaagccgc caccagagaa gccgccgaat ccaccaaatc cgccaccagc tccaccagca
    16381 ccgtaatttg gatctgtgcc tgcatgcccg tattggtcat atgcagcgcg tttttgcggg
    16441 tcacttaaaa tctcatatgc ttctgatact tctttgaatt tttcttccgc atcaggttct
    16501 ttattgatgt caggatggta ttttttcgaa agcttacgat aagctttctt tatttcatct
    16561 tctgatgctc cttttgagag ccctaagact tcataataat cacgttttgt tgccataggc
    16621 tttcctccta ttggttctca tcattttcac gtcgcttgtt atcatatcat attttgtttg
    16681 aaacaaaaac cgatacttca aactattttt caaaagaaaa agccaaagta gcaactttgg
    16741 ccttctctcg aaattcagtg aactgattat ttatcgtcgc catcgacttc ttcaaaatca
    16801 gcatcaacga catcatctgc tccgccttga gctgcttctg ggttttcttg agcttgttgt
    16861 tgtgccgctt gttcgtatag tttaacagtc aagttttgaa cgatttcgtt caatgcatcg
    16921 cgtttagttt tcatttcttc gatgttgttt gcttcaacag cggctttcaa ttcatcgcga
    16981 gctgtttctg cttttttcac ttcttcttcg tccactttgc cttctaattc tttcaatgtt
    17041 ttgtccacag agaatagcaa tgcatctaca tcattacgta ggtcaacttc ctctttacga
    17101 gctttatctg cttctgcatt tgcttctgca tcttttacca tacgttcgat ttcttcgtct
    17161 gttagacctg aagaagattt gatcgtgatt ttttgttctt tttgtgtacc tagatctttc
    17221 gcagatacat ttacaatccc gtttttatca atatcgaatg ttacttcgat ttgtggaata
    17281 ccacgaggag ctgctggaat atctgtcaat tggaatcttc ctagagtttt gttgtcagca
    17341 gccattgggc gttcaccttg taatacatgg atgtctactg ctggttgatt atctgccgca
    17401 gttgagaata cttgtgattt acttgttgga atcgttgtat tgcggtcaat caatttagtg
    17461 aacactccac ccattgtttc gatacctaat gacaatggtg taacgtcaag taaaacaaca
    17521 tctttcacat caccagtgat cacgccacct tggatagcag cacccatagc aactacttca
    17581 tcagggttaa ctgatttgtt tggttctttt cctgtttctt tacgtactgc ttctacaact
    17641 gcagggatac gagtagatcc accgactaaa ataacttcgt cgatttctga ttgtgataaa
    17701 cctgcatctt ttagcgcttg acgtactggt acttttgtac gttctactaa gtcagctgtt
    17761 aattcatcaa attttgcacg tgtcaatgtc atttccaaat gcaatggtcc agattctcct
    17821 gctgtgataa atggcaagct gatttgtgtg cttgatacgc cagataaatc ttttttcgct
    17881 ttttcagcag cgtctttcaa gcgttgtaaa gccattttgt cttgagacaa gtcaatgctg
    17941 ttttcttttt tgaattctgc taccatgtag tcgatgattt tgttatcaaa gtcgtcccca
    18001 cctagatggt tgtcgccagc tgttgacaat acatcaaaga caccgtctcc tagttcaagg
    18061 atagatacgt caaaagtacc gccaccaagg tcgaatacta agattttttc gtcgcgatct
    18121 gttttatcta aaccataggc aagagcagct gcagttggtt cattgacgat acgttctact
    18181 tctagaccag cgatcttacc agcatctttt gttgcttgac gttgtgcatc attaaagtaa
    18241 gctggcacag tgataactgc tttttcgact ttttcaccta aatagtcttc tgcgaatcct
    18301 ttgatatatt gaaggatcat tgctgagatt tcttgtggtg tatatgattt accttctaca
    18361 tctactttgt aacctgcttc accgatatgg cgtttgattg atgaaatcgt atttgggttt
    18421 gtcacagctt gacgttttgc aacttcccct acttggattt ctccattttt gaatgataca
    18481 acagaaggtg ttgtacggtt tccttctggg ttagcgatga tttttgcttc gtttccttcc
    18541 aatactgcta ctgcagagtt agtcgtacct aagtcaatac cgataatttt actcataatt
    18601 aacgtctcct tctttaattg aaagtgtaag ttttatatcc gcacaattat tgtgcaacaa
    18661 tgaccatggt tggtcttaac acgcgatcat gtaatttgta gccttcttgt agtacttcta
    18721 caatcgtatc ggctggtgta tcttcagtcg ctggaacagt ttgtactgct tgatgaagat
    18781 taggatcaaa ggcttctcct ttagcaggga ttttttcaat tccttcttct tccagtgcgt
    18841 tacgcaaact ttccaagacc atttccacgc cttttttcaa acctgcccct tgatcatctg
    18901 acacttctgt ggctaatgca cgttccaggt tgtcaattga tggaagtaat tttttggcta
    18961 aatcttgtga acggtatttt tgtaattgct cacgctcatt ttttcctcga tttgccatat
    19021 ttgcgatttc agcacgagcc cgcaagtatt tatcttccat ttcttctaac tcagctttta
    19081 atttcgctgt ttcgaattct tccgcttcag cttctgaaat acccgaatct tctgcagctt
    19141 cgacatcgat ttccggttct ggctgagcat ctgtcatttc tttatctagt tcttcttgat
    19201 tttctttcac gatacagtct tcctttcatt attcagtcag ctgtttgatt gctcttacta
    19261 agaaagagag aacgatcagc tgcaaatagc ttcgttattc acattacgag tttgacaagt
    19321 caagcgaacg gtagtaatca gcaagtttag ctgctagttc tcttcgataa acatcgacta
    19381 gaccgaacat ccgagaataa ggcatactag taggccctaa caaggctatt gtacctcttc
    19441 catgaccatc tatctcgtaa cttgcttgaa tcatactcat attctgcaat aaatcattgc
    19501 ctaattcagc accaatcttg atatgaatat tgctatccat cggagtgatc atttgtgtaa
    19561 gtccgtctga gtctttcata aaagaaaaca tcgatttgaa ctgatcaaga tcttgacggg
    19621 gctcataatc caagagattc atttgtccac tgacataaac cttatcttca aaaacatgac
    19681 caagaacggt gtcaaataac tgagacatcc cttctgtcgt ttggaaatat ttatgaagaa
    19741 tcatcggaat ctccgtacga agtttatggt acaccgtcat cagaggatct ccgactagac
    19801 ggtcgtttac aatctgtacc attttttcta gttcctgact tcccatatat tcgggaagag
    19861 tgaacacttg gctttctaca ttccccatat ctgttacgac gatagcaata atctgtcgat
    19921 tattcaaggg tacgatacga aagccagtca gtcggcggtc ttttatttca ggtcccaatg
    19981 aaaaagtcgt atagcttgtc agctcagata atatctcagc agattgctta ataatctcat
    20041 tgatttcatg aaattctctg cctagagact gacgaatcag cagcaaatca tcttctgcta
    20101 cgcgagcagg ttttaacaaa tgatccacgt aataacgata gcctaaagca gatggaatcc
    20161 gcccagaaga agagtgagtc ttcaacagca atccttcatc ttctaatgct ttcatatcat
    20221 tgcgaatcgt tgctgagctg gccttgactc cctcagacat caatgttttg gaaccaacgg
    20281 gcacgccact actcgtatag tgttggatga tcagacgtaa aatgtcgttt tgtcgctttg
    20341 ttaacatctt cctcacctct cattagcact catatattct aagtgctaat ccacttataa
    20401 atatagcaag aagcccttct cctgtcaaga gaaagactcc attttttagc actctaaaca
    20461 caagagtgct aattctaaat aattgatgta aatccagtta tatcaacgtt tcaatagtcc
    20521 tttattgtat ttttgaactt tttttattta aattcagata aaaaacaaat attctttaaa
    20581 ctatttttca aacaaaaagc gctcaaacac attgttcccc aaaaaggtcc ctcttggtgt
    20641 caaataaaca cggtcttctt cattaacaag taacccttct tgctctaatt cccttagcac
    20701 atcaccgtac accgattcaa ttgttttttg atagcgatca tagaaatgtt ttttagtaat
    20761 ccctgttttt tttcttagtc ctaagaacat ttcttcttct atttgatttt ttcgtgtcaa
    20821 tttttcttct tccaataccg ggcgttcatt tgctcgtaat gggcgtaaat agtgttggat
    20881 tggtcctttg ttgcggtaac gaatatctcc aagataaccg ctagcaccag ctccaaaccc
    20941 aaaatagtgg tcattgttcc agtacatgag gttatgttga gattcatgtc cggagagccc
    21001 aaagttactt atttcatatt ggtgacgtcc atgcttttcc attgcttcaa tcgtctcttc
    21061 aaacattctg gtttccgttt cctgatcagg cagttcgagt cttccttgtc gtacccagtt
    21121 catgaacatt gttttgtttt cgaggatcaa tgagtacaaa gaatagtgag gcagatccaa
    21181 agccaaagct ttttccaaag tatccagata tccttctagt gtttgtcccg gtaatgcata
    21241 aatcaaatcg atgcttacat tggtaaaatt ttctgcttct aaaaattgca tagtttgata
    21301 cacatcttct gcggtgtgtt tacgtccaat ttttttcaat aagcgattat caaatgtctg
    21361 cactcccatg gataaacgat tgacgccata gtttttcatt acttgaagtt tttcacgggt
    21421 caaatcacct ggattcgctt ctacagtaaa ctcattacca ttttgaaaag gcaggatctc
    21481 tctagcacct gataacagtc gatcaagttg tttagcagat aaagaagttg gtgtcccgcc
    21541 ccctacgtaa agtgtttcta aattcgctgt cggatgactt tctagcatta ttttcatttc
    21601 attcagcagc atttccacat actcatctac tggctgccct tctaaaaaaa ctttattaaa
    21661 atcacagtaa tagcagatat gctcgcaaaa tgggatatga atgtacgcag aaaccttgtt
    21721 ttctgcggga tttattgttt ctatcattga aattctttct ccttacgtcg aaaagtcgtg
    21781 cctgttttcg accttcttaa aatcgcgttc aagcaccaac tcattgaaat aagccgcata
    21841 tttagaatca aggaaaccta gctctaaaaa tgcttgctta ttgtcatcgg agctacacgg
    21901 tgcttttaca gcctctcgat aaacggtaat cgcaaagctg accttcggca ttaagtcaaa
    21961 atctgcaaaa atatgaaaag ctattttcac agattcctcc ttaagtgctc agaccacaac
    22021 gctttgctta caacctctca tatacgcata ctaaacgtat ttggcgaaca aatccactaa
    22081 aaaatccaag aatcgacttc ttggatgatt ttttctttca ttaaactggt tacctagtta
    22141 tatatctcta gggttgtttt catgtctgag tgcccgatat gatttatttt tcgtttctat
    22201 aattactttt tgccttccag tttcttttaa tattgtactc aagcaagtgg gaattggttc
    22261 ttttgtcatc catgtatgca aaatgttata atgctggtaa acaaaaatga ttacctgtca
    22321 tttttattta gttcgtattt aattaagttg actatctgta tactctgtct aaaacttaag
    22381 aattgttgtt ttcgctgtcg ctacccaatt ttaatgaatg tctgtaggtt aaaactgttg
    22441 tccttgttat agatagtaaa ggagagcttg aaaatgaaaa ctgcgattac gtttctatta
    22501 gaggaatatg ccgattggga aggtgcttat attactagca cactaaacca aaatgagaaa
    22561 tggcaaaaca aaactgcttc tatccaatca gaggtcactt ccattggagg attaaaaaca
    22621 aaaatcgatt acttaatcga acaaatacct gaaaaagttg acctttttat tatgatcggt
    22681 ggcaattcat gggacataaa aaatcatcat ttatatcaaa taatcgataa attgttaaaa
    22741 acagacgtac cagtagctgc tatttgtggg gcagttgatt atttagctca gagcggatta
    22801 ttgcaaggtt acaaacacac cggaaatgcg caatttcctt ggttagaatc ggaaaattat
    22861 cagaataaag aagattttat agaacaacaa gctgtgaaag atcgtaattt gattactgcg
    22921 aatggaacag ctcctataga attcacagac ttgattcttc aagcagtcca ttttgaaaca
    22981 gaagaaataa gagaaaaaac gattcgtcta tacaaacttg gattctatga ttactataaa
    23041 aagtacggag atcctttctc ctaatcattc tcttcaatta aatactcctt atcaatagtg
    23101 tgacaagtaa ttttactgat gattatttgt cacacttttt ctgacattta tcaaagaaga
    23161 tacactctat ttatattcat agacaaggtt ttacggattc aaaaattcag gattatgcta
    23221 tactggtatt aaaaaaataa tgaggtattt tatatgaaaa agttaggttt tattttattg
    23281 ttggtctctt tattccttgt aggatgtacg tctcctagta aagaaaatag tgatccatct
    23341 gcgaccacag acaaaagttc aatcagacaa accagttcag ctgaaaaatc aagtactcag
    23401 aaatcagctt ctactgaaac gaatacatct tcaacaagca gtaaagtctc agagcccaat
    23461 acttcttcta catctagtag tacagttgag acaaatattt cttcttcaac aatagacaca
    23521 acctcctcac agtcttctca gtcaatagat ccgttagcag gatattctga tctacaaatt
    23581 gagtacgcac gtgtttggct agctattaga ggtaatactt ttagagatac atcgggtgat
    23641 ttcgaattac atgttattca ttatcctgca ggaacaccta tcgcccctta tgatgcaggg
    23701 agtgcagtct atccgaaaga tgtggttatg ctcactggga aatacagtta ccagggagtt
    23761 ttagtgtatt caagtaacca tgacgggaca atcactagtt atccagttcc ttcacattgg
    23821 caaatgcctg cagatcaaac atcttatcca gcgttcatta gagaaacaac tcaagaaatc
    23881 attgataatg catctgtagt agctattcct acaggaaatc ccaatgacgt gaaacagtta
    23941 atcgatgtga cagttataga taattagact ttctaatatg ttatacaaaa agaagctgac
    24001 agcattttgt cagcttcttt cactttcttt tctatttaga tttattggtc tttgatagac
    24061 tccaaggcag gaaaaaattt tttaaataaa cgtttgcaaa acagtcgctt ttctatcttc
    24121 taccgctact tctgcatagg caccattaat caatgtcagt tgtggcggta catgaccaca
    24181 atcaatatca tacaagatcg gtagttttaa ttcctttgac agatcttcgt atacatcctt
    24241 cattttataa tctaacaaat cctctgccgc acttctaccg aacatgatcc ccgagctgcc
    24301 atcaaaccag cctgccagtt tcatctgaac aagtgatctt cttaaatcaa ctgacgatag
    24361 ctcacagttt tctagaaacc acaagatcgg ctttccatca ttatactttc ttttaaaaga
    24421 ttggacatct ccataaggag taccgattaa atgcctgatc gtatcaatac aaccacctaa
    24481 taatcgtccc ttcatcctta ctgcatctcc gctgactgat tgccaatttg ttggttcagt
    24541 gagatggaaa acagaagatg atgaatgctc atggggccac actttttgat acttttcaga
    24601 agattgctgt agaatcgatt ctccttctct tgttttcagt acactttccc acattgctgt
    24661 cgtttcatcc aagtactctc ctcttaaatc aataaggtta gttccatgtg cagtggctat
    24721 ccctgttttt aatgtgacag caagtagcaa cgtactaata tctgaataac ctactatcca
    24781 ttttggttgt agagcatcaa aatcgacaaa atcgagaatc tcaatcaata attctcctcc
    24841 ccaagtcgga aagatcattt ggatattttc atcttgcatc atttgattga attcatctgc
    24901 tcgttttctt gctggacctg atttttcctt atcttgcgtc caaagcgtct ttccagtaac
    24961 tacttggaaa gatttttctt ccattctctt taaagctgat tcgagtaacg aatggtgttc
    25021 ctctgatacg cctgaggatg gtgcagtaac cccaatcgtt gcatttcttt ctaataatgg
    25081 ataacttatc atgttgcttc acctccaagt atgcactatt ttacttggtt aattgttttt
    25141 ttcgtttgcc ggcatcaaaa atatcggcat ctcaagtagc tggatgattt tgtttaagtc
    25201 aaccgtttgg atgaccatcg tttgatctgg tctacctggt gcaaaaacaa gtttttcatt
    25261 tgataagagc tcttcatcaa taataatcgg aatctgtttt tcataaccaa acggagaaac
    25321 agcgccctgc ttttgtcccg tttgttcttc catgcgttct gatgaaacaa tagaaagcct
    25381 tttaccgacg atcttcccta atgctttgaa atccgtttgt ttcgtagtaa aagtcagata
    25441 tacgtagtta ttatcttttt tatcttttaa gaataagctt ttggtctctg tacctgtaaa
    25501 tccttgttct ttctttatta ccaaagcatc ttcatttgta taaatggctc gatgatgata
    25561 caactcaaaa tatatatttt cgtgtaagaa tttctttgcc aagtcaatca tttttatgtc
    25621 tcttttctat tttattattt gagacatctt atcatataaa tatagcggtt acaacattac
    25681 ttgtgttttg ttcgtttttt atacataact attgtttttc tggtaaaata aatcaaacca
    25741 cttaatggag gcgttttaga atggaaaaat ttcagccgat catcaatcaa atcaaagaaa
    25801 aagaacatca acaaaagatc aaagaccttt ttgctaagat aacagaaacg tatccccagc
    25861 tagacacaac tgtaaaatgg aaccaaccta tgtttactga ccatggtacc tttattattg
    25921 catttagttt tgctaaaaat catttttcta tcgcaccgga aaaagcagcg attcgtgccc
    25981 ttgaaaaaaa tatccaagaa gcaggctacg tatatacgga taacgtcatc aaagttcctt
    26041 ggaaaagtgt aatcaactgg gaattgattg aacaattgat ttcatttaat attgaagata
    26101 aaaaaggtca tgataaattt tggcgataaa gctttatacg aataaacctt ttactggcga
    26161 ggaaactatt catcacctta tacaggtttt gttcaatcct atccttttta tcatccctgg
    26221 ttaaacactt aattgacaaa gtatctgcta tttttataag gagtctttct tgtgctgaaa
    26281 aaaatcactg attgaatggc tttgaatttt ctacatagta aaattatttt ttcagcaatt
    26341 ttcctgcaaa acttttgaat aaggaaaaaa tacgagaaaa tatcagtgat ctttcataat
    26401 actgcccatt attttcaggt atatttaaat ataatatcac tttaggagag aacgaatttc
    26461 tttagagaaa taaaagttag aattttctga cttgattttt acaatgacct taattaaaat
    26521 cgttctttct acatttcgtg attaagcaaa tatcaactaa gtcagtagaa ccattcacat
    26581 aggagggtga acattgaaag atttttactc agtaaatgaa ttagccgaac aattaggtgt
    26641 tactacacgt tctatcagaa attatcttca tgaaggaaaa ctaaaaggaa caaaggttgg
    26701 cgggcaatgg aaattttctg aacgtaatct ttttgagttc ctatacggag atcaagcaga
    26761 ggaagcagca aaagaaatgc agcgattcat gctaaatgca ccaatcacga tgcgtttcaa
    26821 ccttcaatat cgtgatttta cagcaatcaa tcaattccga gaacaattag tccagtacca
    26881 taacgatgtt tacgcaaata aaaaagaccg tcttttacaa tacgatcttt ataaagacaa
    26941 tcatgcggag atactaatcg gtggtaactt caattatgtc gtaaacttta gtcaatggat
    27001 caatgaaaaa ttattgatcc agacagatat ttcccttgta tcttaaacaa ttaaaaagcg
    27061 ctagaaattc cagagatgtt ggaaattcta gcgctatttt tttataagta cttactgttt
    27121 ggttcaaaca aatatttttt taataaatga aattttacca tctttatcct caaataaaga
    27181 ccgtgaccat gcttcctact acgatcagca ataatccgat aatcgttaat tttttttctt
    27241 ttggtgtctt attttcatgc aggatgtaca taccgcctaa tgtagagata actacgttta
    27301 gctgtgtaat gatatatgca gtagcaaccc cattttcatt tgctgaaaaa atataagcca
    27361 atgctgacac actaaagatg gctccaacga tcgttgcttt ccaactcttg tcttcagaaa
    27421 atgcggttgg tttacggaaa atcacataaa tcactgcccc taaaaagaca ccgatcattt
    27481 gcggaaagaa aatggaaagt ccgcttgcat caactagttt aggcaatgca ctgtataccc
    27541 aataaccaat acttgttgat gctaatagac taattccttt tgtgataccg cctttttttc
    27601 caggatcatc actgattgcc gttaaagcag aaccaaatac aagaagaatg attgctgctg
    27661 ctccaatgat tttccccata cttccagacc attctccaaa agcgaagaca ccaatcaacg
    27721 aggttccgac taactgtaat gcagtcgaaa ttggcatcgt tttagaaaca ccaatcaagt
    27781 tgaaagatgt atactgtcct gtttgtccaa taacccaaaa tgcaccagaa agtaaactaa
    27841 tcaaaaatgt agtcactgaa ataccagctg gagaagcgaa taattgaacg agtaagccga
    27901 caagcaccgc tccaatacca gttcctaata tctcatttgt tggtctgcca ccaatctttt
    27961 tcaaaatcag tggctggatc ccccagccaa ctgccgggat caatgcaatc aataaaccca
    28021 tttttattca cccttatcct tttccacctt ggaaagaagg atataaagtc atcccaccat
    28081 ctgcaaatag tgtaatacct gtaacataac tggattcgtc tgaagccaac catgcagctg
    28141 tcgccgctat ttcttttggt tcacctacgc gtcctagtgg gatcatactt tccaagagtt
    28201 ctttttgttc aggatcatca aattttgccg cattaatagg tgtacggata gcaccagggc
    28261 ctatactatt gattctgatt cccttcggtg catactccat tgctaccgtt tcggtaaata
    28321 attttacccc gccttttgaa gcagcgtaat gagcaaacgt tggccatggg atctgttcat
    28381 gaacagaaga catattgatg atatttcctt ttttattatt ctctgtaaag taatttaaag
    28441 ccgctttcgt tccgataaaa acgccagtca aattgacttt taacactctt tcccaatctt
    28501 caaggctaag ttcatgggtt ggatgctgat tttccatccc agcattatta acccaaacgt
    28561 ccagtgtccc aaaattttct aaagccgtat caagtaaacg ctgtattcct tcttcagaac
    28621 tgacatctgc ttgtacagag actgcttttc cgcctgcctc ttcgattgct ttgactgctt
    28681 tttctgctcc ttctttatcc gagttatagt tgacaactac attcattttt tctttcccga
    28741 atcgttgagc aatcgctgtt ccaatcccct ttgagccacc agttactact gcgactttgt
    28801 tttctaattc cttgtacata gtacccactc ctttttcata cctaaacctg ttattacgga
    28861 taacttttat ttccgcctta aagtgagtgt acaccaagat atatagtcta aagcaaatga
    28921 tacgctctag caatcgtttt catgaattgc tagtgtcctt ttatttctat aaagaaagag
    28981 gttgtgacag aagtggtcag cttcaagaaa tttcggctta tttccgaaga agctacttct
    29041 gaaaaaccat ttatttggaa aaaaaggctg tgacaagact tttgtcacag cccctttcct
    29101 gggcttattt atcacccatt ttattgtaat tgctagttat acataaacaa gagattttat
    29161 gcataatgtt ttattgctga gaaaaatatt cagctgttgc ttgttcatcc tccttgagtt
    29221 gttcaatcaa ttcctctaca ctagaaaatt tcacttgatc tcttaaaaat tgattccagc
    29281 gtaccgcgac atgttcacca tagatatctt gattaaagtc caaaatatac aactctacag
    29341 tcagctcccg tccttcaccg aatgtatcat tatgtccaat tgaacccatg gctggatacc
    29401 atgtttcccc aatttttatt tccgacacat aaacaccttc tttaggcaaa tgaaccgtgc
    29461 tctttacttt gacatttgct gtagggaatc ctagtagtct gcctcgtgca tcgccatgga
    29521 caactacccc ttctacctca tatgtgcaac ctaatagttt tgctgcttct tttacttgtc
    29581 cgctgtctag taatccacga atacgggtag agctgatttt ttcaccttgt tcttcttctt
    29641 tagaaactgt gacgattttg aatcttcctt tggcatattc aggaaaatga gcgacatctg
    29701 caatgtcttt tggtccatac gtataatcaa aaccggaaac agcatatttt gcatgtaacc
    29761 ctacgatgta ttgatcaaca aattcctgcg gtgctaaatg agcaaaagaa gaagtaaaat
    29821 caacttcata taaataatct atacccaaag cggccatttt ttcttctttt tgcttaaggc
    29881 tagtcaaata tttcacgctg ctaggatcga tctttttaaa gacaatagat ggatgctgat
    29941 tgaaagtcat caatgctaat ttcaatcctt ctttttgtgc aatttccata cctgtcttta
    30001 tgactttctg gtgtccgata tgcacgccgt cgaaaaatcc cagcactaaa acgacatcgt
    30061 cttctggaat ttgttctgat tggtaaggat gtctgatctt gatgatattc attcgttatt
    30121 cctcatttct caatacttta ctaggtttta atttgttttt ttctttcggg tttggctgat
    30181 aaatactgac aacttttcct ttataaaaca aagcaatttc ttctgacggc atttctggga
    30241 gcgcaaatac agtataatca agtctcatac cgttttttac tttctgccat acttcatcag
    30301 taatatctac tcgtttgaac cgttccactc ctttttctat tggcagcaaa caatcatcga
    30361 ttgtcccatt tttcacagct tgagctactt cttctaatgt cagagcatcc ttctgggaca
    30421 taccggcact tgctgttcgg gtcagatcag acatgtgcgc agggtaacca agtttcttgc
    30481 ctgtatctac agctaatgtg cggacatagg ttcctttccc gcattctact tcgaagcgcc
    30541 aagaaatggt tcctgtcact tcgtctcttg tcaattcact tgtccggata aaacggtaaa
    30601 tctgagcctt tctaacagga cgctccactg tttcattatt gcgggcatat tcatataacc
    30661 gcttaccgtt gacttttacc gctgagtaca ttggtggtat ctgggtgatc tcaccaataa
    30721 atgaagccat cacttcatct attttttttt cagataaaga catatctact ggttttgttt
    30781 ctacccgttc tccggatcga tcttccgtcg ttgtactgaa acctagagtt atctcgccta
    30841 catagatttt cccagaatct gtcaaatatt cgataacttt tgtggctttt ccaatacaaa
    30901 tgggcaacac accgtctacg tctgggtcaa gcgtaccccc atgtccgatt tttttcgtat
    30961 gtaagatttt tcttaatttg aatacgcaat catgacttgt cattccgcgt tctttccata
    31021 aaggtaataa cccgtccatg tcttttctcc atttcatttt tgatttaact gaattattat
    31081 aacacattgc atcaattact gacgaatttg acatcataat gaacggtttt tatttttcgg
    31141 caataaaaac gtaacaaaat tcatgctcac gtaaatagaa atcatgttat gcttttttaa
    31201 aggagggaat agaatggaag attttttctt tgcaccattt gattggattt atgggtggct
    31261 tacaggtaaa tagtttggtt ttcacttccc aaaaacgaaa aaaaagactg gccattcttc
    31321 cagtcttttt tccgtttttt ttatttattt tttcagtttc ttaatcaatg taaaaaattc
    31381 tattttgcgg gatcgatttt atcattttat tggtcaagat ttttccattt ccaatccaat
    31441 acttctggca gatcaatacc attttcacga atatagtttt gatgttcttg taatttctga
    31501 tccatttttt tcgcaaaatc ttctgcttga cttcctttga tttttaaagc tgcgtctttt
    31561 gccaaatgat aacgatcaag ttcatttact acacgcatgt caaatggtgt cgtgatatct
    31621 ccattttctt tgtagccatg aatcattaaa tgatggttat gacgatcaaa gaaaatcgtg
    31681 cggaccaatg tttcataacc gtggaaagca aataagatcg gtttgtcttt tgtaaaatat
    31741 tgatcaaatt cttcatcgct caacccacgc ggatcttgac ttggatgacg taatttcaat
    31801 aaatctacga cattgatgaa ccgaattttt agttttggga attcttccag cagcaaagtt
    31861 acagctgcta atgcttctaa gttagattcc gttcctgcag cagcaatgac caattcaggt
    31921 tcctcctctt ttgctgtcga agcccaatca atgattttca agccttcatt cactaagacg
    31981 gctgcttctt ctgctgagta aaattgagca cgcggatgtt tggaagagat gatcaaattg
    32041 attttttctg acgagcggaa tgctttatcc atgactgcga gcaatgtatt tgcatccgca
    32101 ggcagatatt cacggatgaa ctccgccttc ttttctgcca aatgtgtcaa tattcctgga
    32161 tcttggtggc tatatccatt gtgatcttgc tggaagactg ttgagcttgc aatgagattt
    32221 agcgaaggat aatcatttcg ccaagaaagg tcatgtgatt tccgcatcca tttgaagtgc
    32281 tgagtcaaca tagagtcaac cactcgcaag aatgattcgt aacttgcaaa aaaccatgcc
    32341 gtccagtcag aacgtaccct tctaagaacc cttcatcttg gtgttccgat aattgactgt
    32401 cgatgacgcg accagatggc gaaagccatt catcattttc tggttcgatt ttttccatcc
    32461 attggcgatt tgtcgttttg aaaacttgat tcaatcggtt tgattttgtt tcatctggtc
    32521 caaatatacg gaaattttct ggatttttct caatgatctc gcgaacgaat ttaccaagtt
    32581 cggtcatatc ttctgcttta acggagcctg gtttttcaaa ttgcaaagta tattttttcc
    32641 aatctggcat aataagcggt cttggatcga ttccaccatt ggtgatagga ttttttgcca
    32701 ttctttggtc accagtaggc gcgatttctc tgatttcttc taaaatacgt ccttgtgcat
    32761 caaataattt ttctggttca taggatttca gccaacggag taaagcatct gcatgatcca
    32821 tatgttcttg atctacagga atcggtactt ggtgtgctcg gaaggttcct tcaattggtt
    32881 ccccgtccca ttcttttgga ccggtccatc cttttggtgt gcgtacgatc aatactggcc
    32941 attttggcat cacgacatct gttgctgtct tcaggcgagc ttctttttgg atggatttaa
    33001 ttttctcgat tgcttgatcc attttttcag ccataacagg atttaatttt tctgaatcat
    33061 ttccttcaat aaagaaaggt tcccagccaa gtccgttaaa gtaatttgct aattctccat
    33121 ctgatttacg tgccaagatc gttgggttgg ctatttttgc accatttaaa tgaaggatcg
    33181 gtaaaacagc tccatctatc actggattga taaagacatt tgagaaccag cttccagcta
    33241 aaggaccggt ttctgcttca ccatcaccaa tcacgacagc agagattact tcaggattgt
    33301 ctaatacagc tccgactgca tgtgaaagag agtatccaag ctctccgcct tcatggatag
    33361 aacctggtgt ttctggtgct gcatgagaag caatgcctcc aggaaatgaa aatcgtttga
    33421 ataatttttg cattcctgtc tcatcttccg ttacttctgg atagatctct gtgtaacttc
    33481 catctaaata agcattcgat accatgactt gtccgccgtg tcctggccct tcaatataaa
    33541 acatgttcaa atcatattta ttgatgactc ggttcaaatg gacataaata aaattttggc
    33601 caggaatcgt tccccaatga ccgattggat gttttttgac atcttcgggt ttcagtgtac
    33661 gtctcagtaa ggggttatct ttaagataaa gttgcccaac agaaagataa ttagcagcac
    33721 gccaccaagc cgtcatttta tcaaagtatt ctttactaga ataatccata cataacactc
    33781 cttttcatta ttactcttat tctagcgcag atactcccaa ttgaaaccta ataagcactt
    33841 ctgttcattt gtttggattt tatatctagc caaatgaaca ggtataaaaa accacgaaat
    33901 ttttcctttc gctgacgttt tttacattga tccaaaaaac ttgtcgttat cacaatggct
    33961 gttccgcagt tcatttcgct cctcatcatg cataatttgc tgaatgacgc tgggccagtt
    34021 aacacattgc tggaaaactg gggctggatc gagcaattcc tttcctgatt gatccgtttt
    34081 atgtaaaagt ctctgttatc tttgtcaata tgtgaattgg tatcccagct actatgctaa
    34141 tttctacagg gattatccaa aatttggatc gtgaccaaat cgaagcggcg taatcggaaa
    34201 tatcaataac ttcaacgtga tttttctttt aactgtcaac ggaccgacaa actcaaactt
    34261 ttatggtgca ggttccactg atcttctagt tacttggcta tacctgactg tcactaaaat
    34321 agattacaat ctagcttccg ttatcggtat cttgatcttt atctatatgc aaaaatttat
    34381 atactactag aaaaaagaaa gtcgaatttt ttacttttct ttttctcttt tttggttagc
    34441 tgacacgccg caagactaaa gtcatggaac tcttaggtaa tacgtcttaa tgaaaacaaa
    34501 aaaatcccgc aacccctgat ttcacaggat ttgcgggagt ttttgattag tctttgttca
    34561 agtcgcgaag caattcatcg atatggttgc catagacaac tgattcatca agttcaaaga
    34621 acaattcagg tgtcttataa atacttagat tgtgccctaa ctctcgacga atcaatcctg
    34681 atgctttatt caatccatct tgtgctgttt tcttatctga tgctaagtca gataaaatgc
    34741 tgtaatagat cgttgcttgc tgtaagtccc ccgtaacttt cacatctgtg atcgttacgt
    34801 tctccacacg agggtcacgg acctttttgc gcaaaatatc attgacttct tttaagattt
    34861 cttgaccgac tcggcggtca cggtagttag ccataatgat tggcctcctt ttatcagttt
    34921 ttagtcattt ttgacttctt ccatgataaa tccttcaatt acgtcatcta ctttgatgtc
    34981 attgaatttt tcaatcattg caccacattc ataacccatt ttgacttctt tcacgtcatc
    35041 tttgaatcgt ttcagacttg caagttggcc ttcatagatt acgattccgt cacggattac
    35101 acggacaccg ctatctctac gaatatatcc ttctgttaca taggcacctg caatcgtacc
    35161 aactttagaa actttgaagg tttcacgaac gatcatttga ccggtgattt tttcttcaaa
    35221 ttcagggtct agcatccctt tcatcgcagt ttcgatttct tcgatggctt tatagatgat
    35281 acggtgaaga cgaatatcga cttcttcagt atcagcttgc acttttgcct gcggtgtagg
    35341 acgaacgttg aaaccgatga taatagcgtt gctggctgct gctaaggtta catcactttc
    35401 attgattgct ccgacagctg aatggacgat ttttacacgg acaccttcca catcaatctt
    35461 cttcaatgat gcagccagtg cttctgctga accttgtaca tctgctttga tgattacgtt
    35521 aacttctttc aattcgcctt ctttcaggct ttcaaataag ttatccaaag tcacacggct
    35581 tgtggcagca cggttttcta ataatgcacg tttcgcgcgt tcttcacccg ctgcacgtgc
    35641 tgtcttttcg tcttcgaaga ctacgaaacg gtcacctgct tgaggtacat cattcaagcc
    35701 agtgatttct actggcgttg caggtcctgc ttctttatcg cgacggccaa gatcgtttgt
    35761 cattacacgg acacgtccgt aagtattacc gacaacgatt gggtcaccaa catgaagtgt
    35821 tccttcttgg acaagtaagg ttgacacagg acctttacct ttatcaagac gtgcttcgat
    35881 cactgttccg attgcgcgtt gtgttggatc tgcttttaaa tcttctactt ctgcgactaa
    35941 caggatcatt tccagcaatt cttcgatgtt ttggccgaat ttcgctgaga tctcaacaaa
    36001 gatcgtttct ccaccccatg cttcaggaat taactcatac tcacttaatt cttgcataac
    36061 atgttgaggg tttgcacctg gtttatcaat tttatttaca gcgacgatga ttggaacgcc
    36121 tgcagctttg gcatggttga ttgcttcaac tgtttgtggc atgacgccat catctgcagc
    36181 aacaactaag atcgtgatat ctgtgatact tgctccacgt gcacgcatac ttgtaaaggc
    36241 cgcatgtcct ggtgtatcca agaaagtgat tggtttacca tcaatatcga tctggtaggc
    36301 accgatatgc tgtgtgattc cgccggcttc gccgcttgtt acacgtgaat ggcgtaatgt
    36361 atccaataaa gttgttttcc catggtctac gtgtcccata atcgttacga ctggcggacg
    36421 tgagactaat ttatctggat cgacttcttc tgcctcgaag aatttatcga tatcagcaat
    36481 gtcaacttgg actttttctt gtggttccat gccatagtcc gtcgctaaca gttcaatcgt
    36541 atctttatcc agtgcttggt tttgattgac catgacacca agcataaata attttttaat
    36601 gatttctgct ggctcacgat gaattttttt cgcaatatct gctacgttca taccttcagt
    36661 atactctaat acatctggca attcacggaa tttgcgtggt ggtacagctg gtttagttga
    36721 ttgttgctgt ttgccttttt tacctttttt attataacga ttacggttat tattattaaa
    36781 tttgccgcga ttgttattgt ttggtttgtt gttattgtta gtacggtttt gattgccgcc
    36841 ttggctcgtt ccttgacgat tttggctgct ggtatttccc gtattatttt ggtttgttgt
    36901 tggttttttt ccttgattca cttgaccgct cccttgaccg cgatcttgat aactgcggtt
    36961 atttttattt ttttgttgat tgttcgttgt ttctttttta cgttgatcgt tcggctgttg
    37021 attggctggt tttctgcttg cttgttgtgc agcttgttgt ttcgtgcttg ggtttttgaa
    37081 cgcttgttgc aatttcttct catcattgct agtaatcgca cccatatggt ttttcacatc
    37141 gatgccaagt tgttgtgctt tttccacgac gtctttacta gattttttga gttcttttgc
    37201 taattcatag attcttttgt tgcccatgca atcaccttcc taaccttcaa ttagctcctt
    37261 ggtcttcttc gcaaatccgg catccattat tccgaatacc ttgcgaggtt ttccgatcat
    37321 ctgcgtgatt tctgcttcag aaaatagctc aaagcaagga acttcataat atgaactctt
    37381 atcttttatc ttttttcttg tattctcgct tgcatcggtt gccacaaaca ccaattttgc
    37441 tttttgtttg cggatttcag caatggtcag ttcttcaccc gtgattagtt ttcccgcgcg
    37501 catcgctaga ccgatgagat tcatggcttt ttgccgatta actgtattca ttttccaaat
    37561 aactctttac gtgctttttg gtgagtgaca tagtcaagaa gttcttgata aaactcatct
    37621 gttaatgttg tttcgagtac tcgatcgaga atttttttat cccaagcttg ttgtacttct
    37681 tccggctcaa gtgagacata cgctcctcgt ccaggcattt ttccagttgg atcgatcgat
    37741 acttcccctt cttttgaacg ggtgattcgg accatctctt ttttaggttt catttcacct
    37801 gaaaccacgg atttgcgtaa aggaattttc ctttgtttca tctctttgcc tccttactaa
    37861 agttaagctt ctggtttttc ttcgaccgtt tcattgttga atgcaattgt ttcatattca
    37921 tcgtctgtta aatctgactg aacgattgct tcatcatgca gttcttctgt ttctttttgt
    37981 tgttcttgat tttcataaaa ttccgtcatg tcagattctg atttgatatc gattttatgg
    38041 ttagtcaatt tagcagctaa acgtgcgttt tgtccgcgtt taccgattgc tagcgataat
    38101 tggtagtctg gaacgacaac tgtgcaggcc tttggatttg tctcatcgaa gatcacatcc
    38161 atgacttgtg caggattcaa tgcatttgca atgaatacag ctggatcttc attccattcg
    38221 acgatatcca tgttttcgcc ttttaattca ttgacgattg cttgaactcg ttgtcccttt
    38281 ggcccgacac atgtaccgac tgcatcgata tttggatcat ttgagcgaac tgccactttt
    38341 gaacgatcgc cagcttcacg ggcgatgctg acaatttcaa cgattccatc atagacttct
    38401 ggtacttctt gctcaaataa gcggcgcaat aggtctggat ggctgcggct aacaaatact
    38461 tgcggacctt tagacgtatt ttctactcgg gaaacataaa ctttgattcg atcatgtggt
    38521 tgataaaatt catttggcat ttgatcttgt ttagaaagaa ctgcctcaat tttaccaaga
    38581 ttgacataaa tataacgttt atcctggcgt tcgacgattc cttgcatgat gtctttttca
    38641 taagcactga attcgttata aataatcgtt ctttctgctt cacgtacacg ctgtaaaata
    38701 acttgtttag ccgtttgcgc agcgatccgc ccaaaatctt ttggtgttac ttcaaaacgg
    38761 atcgtatcac cgatctcata tgctggattg attaaaagtg catctttcaa tgagacttcc
    38821 aactgtgaat ccatcacttc ttccgttact tcttttaccg catagacatg gatctttcct
    38881 tttttctgat caaattccac ttcgacgttt tgtgcttggc catagtgacg tttgtatgca
    38941 gaaaccaatg ctgcttctaa agcatcgatc acgatttctt ttgagatccc tttttcagct
    39001 tctaaagcgt ctaacgcatt taacatttct ttactcattt tttctgattc ctctccgtga
    39061 ttaaaattgg atagctaagc gagcttttgc aatatttttt cggtcaaaag tcaattcttt
    39121 ttctcgtgtt tttatgcgta ttttcaatgt aagctgttca gcatcaaatg attgaagaaa
    39181 tccttcatat tgtttttcgc catctactgg ttggtataag gagacatgga cataatcacc
    39241 aagtgccttt tcatagtcgc tttctttttt caatggccgc tctgctcccg gggaggatac
    39301 ctccaggaaa taagcttgag gaatcggatc tggatctgct gcatcaagtt tttcactcag
    39361 tttctcactg acaaaggcgc attcttcgat atcgataccg ccttctttgt caataaatac
    39421 tcgtaagaac cagctttttc cttcttttac aaattcgact tcgacaagtt caaaattttg
    39481 ttcatctaaa atcggtgtca ccatttctgt gactgtttca acgacgctac tcaaaagttt
    39541 cgcctcctta aaaaattaag tgcgtaattc cattaaaaag agtgagcgaa aaatccgctc
    39601 actcacagtt agtatcatta cctataagaa ctataccata ctctgcttaa aattacaagc
    39661 agacaggaaa acagatgctt atagcatatc gaataacgat aattgatttt catctggtag
    39721 atctttcaat acgccattgt cgttcatata ttcgatcaat gtttttgaaa ctttaccgcg
    39781 ggtggcaaga tcttctttgg ataaaaatgg accgtctttt cttgcttcaa cgatttgttt
    39841 tgccacgttg gttcccaaac ttgggacagc tctaaatggt gcgatcaacg tatcaccttc
    39901 aataacaaag ttaacggcat ccgatttata caaatcaatc attccaaatt tgaatcctcg
    39961 ttccagcatt tcattggcta actccagcac tgtcagttga ttcttttctt ttacggaagc
    40021 atctaatcct ttgtccgtga tttctttcat tgcttgtttc acagcatctt ttcctttgca
    40081 catggagact agatcaaagt cgtctgcacg tacagaaaag tatgcacagt aatacaaaat
    40141 agggaaataa accttgaaat aagcgacacg aagagccatc aatacgtaag ctgcagcatg
    40201 ggctttcgga aacatatatt tgatttttga acaagaatcg atataccagt cgggcacatt
    40261 attttctttc atcgccgata gataggtttc tcgcaattca tcagggatct tgttccattg
    40321 tcctttacga acagtttcca tgattttaaa tgccatcccg ctatctaatc cagcatggat
    40381 caaataaacc ataatatcat cacgacagcc gattacttcc gccagtgtcg catctcctcg
    40441 tcgaatcaat tcttccgcat ttcctaacca tacgtctgta ccatgagata gtccggaaat
    40501 ttgcagtagt tctgcaaaag ttgtaggatg cgtttcttct agcatccctc gaacaaaacg
    40561 agtgccgaat tctggaatac ctaatgtccc tgttttggaa taaatttgtt cctgacttac
    40621 tccaagtact tcaggaccgg caaatatccg catcacttcc gggtcatccg tagggatcgt
    40681 ttgtggatcg atccctgata aatcttgaag catccgaata acagttgggt catcatgccc
    40741 tagaatatct aatttcaata cattatcgtg aatcgagtga aaatcaaaat gagtcgtttt
    40801 ccattccgaa ttttgatcgt ctgctggata ttggatcggc gtaaaatcat agacatccat
    40861 ataatcggga ataacgatga tcccccctgg atgctggccg gttgtccgtt tgactccagt
    40921 agcgcctttt gctaatctgt cgacttctgc acttcgaaac tgcagattat tgtccctttc
    40981 gtatcctttt acatagccat atgctgtttt gtcggcgact gtaccaatcg ttccggcacg
    41041 gtaaacatag tcttcgccaa acaatacttt cgtatagtta tgggcttctg cttgataatc
    41101 acctgagaag ttcaaatcaa tatcgggcac tttgtctcca tggaatccta aaaatgtctc
    41161 aaatggaata tcatgaccgt ctttgtttaa gcgtgcacca cattttggac attgtttttc
    41221 tggcatgtca aatccagaac catacgtacc atcttcaaaa aactcggaat attggcactc
    41281 tggacaataa taatgagggg ctaacggatt aacttcagtg attcctgtca tcgtcgcaac
    41341 aaagctagag ccaacagagc cacgtgaacc tactagatag ccatcttcat tacttttatg
    41401 cactagtttt tgggcgatca gataaataac cgagaagcca ttaccattaa tagaattcaa
    41461 ctcttttttt aatcgttttt gaatgatctc aggtaacgga tctccgtaca tttgttttgc
    41521 ttttgtgtaa ctcaattctg atatttcatc ttctgaacct ggaatcttcg gtgtataaag
    41581 ttcatctttg actggaatca cttcttcgca gatatctgcg attttattcg tattttctac
    41641 tacgatctct ttcgctgttt cttcccctaa aaagtgaaat gcagttagca tctcatcggt
    41701 tgttcggaaa tggacatccg gcaaactatg acgattcaac ggattcgctc cgcccataga
    41761 attgatcaaa atttttcggt aaatggcatc ttcttcgttc agataatgta cattacctgt
    41821 tgcaacaacg attttatcca aactttttcc gatttctacc aaattttgga taatttcttc
    41881 caaatcatgt tcatttttta ccaattcttg ctcgatcaaa ggcgcataga ccgcttttgg
    41941 cataacttca atataatcgt agaattttgc tcggtttctt gcctcttcta cccctttttg
    42001 catcatggct tcgaatattt ctcccttatc acaagctgag ccgatcaata gattttcacg
    42061 catctttttc aattgggagc gtggtattcg tgggacgcgt tcgaagtatt cgacattcga
    42121 cattgaaatc agtttgaaca aatctttcag tcctgcctga ttttttgcta aaatcgttac
    42181 atggaaaggt cgagcccttt tgtaagaatc tccttctccg atatgctcat ttagctgatc
    42241 atgataaagc atgttatgat tgtccattgc ctctttcaca aagatccatg ctaagtgacc
    42301 agttgcttca gcatcataga ttgctcggtg atgctgttcc aagctgacac caaatttttt
    42361 agataacaca cctaatccga aacgtttgaa ttgaggatac agatatctag ctaattccaa
    42421 cgtatcaatg actggattgg ccgcttcagg aataccatac cttgcgtagc tagtattcaa
    42481 gaaccccata tcaaaagccg cgttgtgggc taccaaaatt gtatcttttg aaaattcaag
    42541 gaacatccgc aatacttctt cttccgactt tgatcctcga accattccat ccgtgatacc
    42601 agtcagatct accgttgttc gtgacaaagg atgtcctgga tcgataaatt catcaaagct
    42661 ttcgatcaca tttcctttat acattttgac tgcggctagc tcgatgatcg tgtcatatac
    42721 tgcagacaat cctgtcgttt ccacgtcaaa tacgacatat gtggcttcac tcaatgcttc
    42781 atgtgcatca ttataagcaa ttgggacacc atcatctacg acattcgctt ccacgccgta
    42841 taagattttc acacctgctt tttttccagc actatgtgct tccggaaagg cttgcgcacc
    42901 accgtggtct gtaatggcga tagctcggtg tccccatttc cctgcttgtg ccacaagatc
    42961 agagatgctg tttgttgcat ccatcgtact catattgcta tgaacatgaa gctctactcg
    43021 tttttcccct tcaggagcat aatctttgcg aggtgtatgt ttgacttcta tgatatcttg
    43081 cgcattcatg accaaatcac ggacaaacgt atcttcttga atacttcctc gtacctttaa
    43141 ccagctaccg acactgatcg catcaaatac ttgttcatct ttttcgccgt tcgagaattt
    43201 tttgacgata aatgatgaag tataatctgt gattttcaaa gtcaagatct tgcgttttga
    43261 acgtagttct ctgacttctt tgtcgaatac gtacccctca agtgtgactc ttcgttcttc
    43321 ttcaacgata ctgatcattg gtgtcgttgg ttcatcggct ggaatatttc gtcctagctg
    43381 gatgtggatg ggtccttcaa gagctggcga ttgctctttt ctttcctttt tcttttgttc
    43441 gtggacaact agactttcag ctgcttgctt cataaaggct tctgcctgtt cttgtttgcg
    43501 ttcttcaaaa agtttcaata ctcgttccgc ttgctgctca tccacttctg gttcgatacg
    43561 aaattttggg aatccgtaac tgacgaacaa ctcttctact aatggcaagt attgttgctt
    43621 caaataagaa atagctcctg tactgtcgat tggcaagatg actttacgat cttttatcac
    43681 aggtacctgc gttttcaata cttgctgtgc tagtggtgta tcgcattgct gattttccaa
    43741 agcttgggcc cagtaatctt gcaagagctg ttcatcgaat gtctggtcat ctgctaagat
    43801 attcagtttg acttgtgcaa tatctttaaa agcgatagtc agatgctgag tcaatgaccg
    43861 atatagcata ataggcaaaa ttttggaaaa atgcagcgta aattcccata aacgcgattt
    43921 acgatggacc actacactat ccatttcacc agcagtaagc agtggatgat ttttttctgc
    43981 ttcctctagt tggatttgtt ccaacagttt ttcaaataaa tctcgttttt ctgacaaaga
    44041 tatacgccct ttctgatgag aaacaatgca attgatcgca tcttatcctc ttctttttca
    44101 gtacctttta gtatactcgt tttttctgag ttttacatta aaaaattgga actgcgattc
    44161 aaatcgcagt tccaatttta aaagcgaatc atcagattgt caaaaacagc tgattcagct
    44221 atttttattt tctgtgtttt aacgcttatt attcgccttg gttcaagagg attggtaaag
    44281 tattcaccaa ttcttcttta cgtacttcaa ccatttcgcc agttttcttg attttcactt
    44341 ctacgattcc atccactgct tttttaccga cagtgattct gattggacag ccaatcaaat
    44401 cagaatccgc gaatttcact cctgcgcgtt catttctgtc atcaaccagt acttcatagc
    44461 cagcttttgt catcgcttct tctagttcat tcgtcaggtt tgtttggtat tcgtctttta
    44521 gattcatttg gacgatatgc aaatcaaatg gcgcaattcc ttttggccag ttgattcctt
    44581 gttcatctga atgttgttct acgattgctg ataataaacg acttacaccg ataccataac
    44641 agcccataat cacaaatttt tctcgtccat tttcgtctag tacagttgct cccatagatt
    44701 cactgtaacg agtgccgatt ttgaagatat gtccgatttc gattcctcga gtaaaggcaa
    44761 gaacaccatt gttgtctggt gaaggatctc cttcttgtac aaaacgaaga tcttcataag
    44821 aaagtacagt gaaatcgcgt tctggattca catttttata gtggaatcct gtttcgttcg
    44881 ctccagtaat ggcattcgct aaatcttgga cataacggtc tgcatatact tttacgtcat
    44941 ctgaaacacc gactggtccg acagaaccaa actcagcacc tagatatttt tgtgcttctt
    45001 cttctgttgc ttcttctaaa aagtcagcag ataaaaagtt tttcagtttt acgtcattga
    45061 cttcatggtc tccgcgtacc aaaacaagga caggttgttc atccgcaatg aacaatacag
    45121 acttgatgat tttttgaggt tcaacagaaa agaaatctgc tacttcgtca attgatttta
    45181 cgttaggtgt agctactttc tccaattcaa gctgtgtttc atgcgatttt tttgatacat
    45241 agtagcttgt agccatctcc aagtttgcag cataatcact ttcagtcgaa taacagatcg
    45301 tatcctcccc gatttctgag atcgccatga attcttttga gtctttcccg cccattgcgc
    45361 caccgtctcc gataatcgag cgaaattcta agccacaacg cttgaatact tcggtgtagg
    45421 ctttttcgta atctttatat gtctcgtcca aactttcttc cgttgcatgg aaagaatacg
    45481 catctttcat gataaactct cttccgcgaa gcaaaccgaa gcgaggtcgt ttttcgtcac
    45541 gatatttagg ttggatttga taaagattta aaggtaattt cttatatgaa ttgatttcat
    45601 tacgcatcaa atcagtaaaa gtttcttcgt gagttggtcc aaggatcatt tttcgatcat
    45661 tgcggtcttt gaaacgatac aagtttggtc catatgtttc gtaacgcccg gattcttccc
    45721 aaagttctgc tggcaagatg gcaggcatca gcatttctac cgctccgatt ttttcaaatt
    45781 cttcccgcat aatgcgtttc agtttttcca atacacgatt tgctaatggc agatatgaat
    45841 aaatacccgc tgatacttgg cggatatatc cagctcttag caagatttga tggcttaata
    45901 cttctgcatc atttggaact tcgcgtaatg ttggaatcaa catttttgat tgtctcattc
    45961 tatatactcc tttatttcct caaaatagtt atcgtttgtt attataccct atttgtcaac
    46021 tttttctaga aaaagaatcg ttgaatatcg ttccatgtaa ctagcaccat caacaacatt
    46081 aaaaatccaa agccagctaa tgtcaatatt ccttcttttt cttgacttaa tggtttcccg
    46141 cgaatccctt caaaaatatt caagactaat ttcccgccat ctaaggcagg aatcggaagc
    46201 agattgacga ttccgagatt cattgaaaga agcgccatca aactaataac agttgttaca
    46261 ccttgatttg ctgcttctga agacaattgg tacatcatga caggtccgcc taatttatct
    46321 aaactgaaac ctgtaaatag tgatcctaag gctttgaaaa tttccaatga accgctgaaa
    46381 gcttgacgcg tccctccaat gatcttatcc ataaaacctg tattcattgg ggcttttata
    46441 ccaagttggc caactttttc gccatttgac tctacagatt tcggtgtcac gtcgacagat
    46501 tgcacctgac cttctcgttc gatcttaaag tctaatgttt tatccggatt tttcgtaatg
    46561 actgttgtca gatcattcca tgaatggatt tcttttccgt ccacactgac tacctcatca
    46621 ttttctttta agcctgcttc tgccgcagct ccattaggca tgatctctcc tacgcgatta
    46681 gtgttcgtca cttgaacacc accttgcata aatgccagaa tgatgaataa caggattgcc
    46741 aaaataaagt tattcatggg tccagcaaag ttggtcagca tacgctgcca caatttagcc
    46801 gattggaact gtacgtcttt tggcgcaatt ctgatttctg ttccgtcgtt ttcaataatc
    46861 gtcgcatcgt gatcaacagg gtaggttact acttctgttt cgtctccatt cacataacca
    46921 gtgatagtca gctcatcttc taaatcataa cggctcaatt ccatcgggat agcatttgtc
    46981 aattggattt tcttacttaa atttatcttc tctacgatgc cgtcagaatt tagtaaaaga
    47041 gaaagaggca ttcctggtgc catttctgtt tcatcatccc cattgcctgc cattcgaaca
    47101 taaccgccaa taggtaatag gcgaagcgta tatgttgtcc cgtcttttgc ttgatgccca
    47161 taaattttcg gacccatacc gatagcaaat tcgcgaacga ggattcctga tcgtttcgca
    47221 aagaagaaat gaccaaactc atgaacaatc actaatatac caaaaacgat gataaatgtc
    47281 agaatcgttt tcatagcttt tttctccttt acttgttttt tgtgtttttt ataaaacaac
    47341 gataaaacac tataatttta ccatagtata ccaaagacac caatagtatc aatataatga
    47401 aaaataaaaa cctgcgtctt ttatgaaatt cacaaagaaa cgcaggtata cttactaaat
    47461 aatgccaaat aaatgcatga taggaaatac aaacagcaaa ctgtcgaaac ggtctaagat
    47521 tccaccatgg cctggtaaga tatttcccga gtctttcaca tcataatgac gtttgattgc
    47581 agattcgacc aaatcaccaa attgtccgac tgctgaaaaa ataatcgtca tcagaagcat
    47641 gacaaacata ccatgattaa atagttcttt tgctgggtat agcaataaat aaagaaatgc
    47701 gactacaaca gcactaccaa taccaccgag cgcaccttca atcgttttgt tgggcgaaat
    47761 atcaggactt aacttatgct gaccataacg tcgaccaacc atatatgctc cgatatcagt
    47821 tgcccaaaca ataaatagac caaatatcaa aacagccaag ctttctgttc gtgcattgac
    47881 aaaattctgg aaacctattc ccacatacaa gctgacaagc acaggaaaac ctgcttcatc
    47941 aatcgtatac gtatttttag aaataactga tgttcctaaa agaatcataa ctataagata
    48001 aaataacata aatgtactta cattctctgg aagaaagaaa aaccatcttt cttttggcaa
    48061 aacaagtaac actgcaccaa cggcggataa aactccttca aaacttaaga gtgtcaatcc
    48121 tttcatccga aacagctcgt aaactcctat tactgctaag attgctgctg taagctcaac
    48181 cgctatgcct ccgatccata taatcggaat aaacaagact agcgccacgg ctgctgtgac
    48241 cacacgttgt ttcatgactg atcccctttc tcatcttctg ttttttttac accgccgaat
    48301 cgacggtctc gattttgata agaggcgatt gcttcttcaa gatgcgcacc atcaaaatca
    48361 ggccaaagcg cttttgtaaa atacagctca ctatatgcga tctgccataa cagaaaatta
    48421 ctaattcgct cttctccact tgtacggatc atcagctcag gatcttgtaa ctctttaggc
    48481 aagaaacctg tcattaaatg cttagcaatc aattcatcat cgatttcttc tgcgtgtatt
    48541 tctccttttg ctacttcttc ggcaatttct tggacagcag tcacgatctc tgctctgctt
    48601 ccataattca aagcaaaatt caataccatt ccagtattgt tttttgtttg ctcaattgca
    48661 cgtcttacag catcttgcgt atgttctggc aatacgtttt cataacccat gacatgaact
    48721 ttgacatttt ctttaatcaa ttctggtacg aacgtatcaa agaagtcgac aggtagttgc
    48781 atgagaaaac ttacttcatc ttttggtcgc ttccagtttt cagtcgaaaa ggcataaagc
    48841 gtcaacactt tcactccaag ccttgatgct ttttttgtga ctttctttac tgtttccatg
    48901 ccttctttat ggccagcaac tcgtggcaaa cgacggtttt gcgcccaacg accattgccg
    48961 tccatgatga tggcaatgtg ctgaggaatt ggtccatcgg tattaaattg aaattcgctt
    49021 ttttcttgaa tatatttgtt tttttgcgga aaaaaacgta acattctttt tcctcctata
    49081 cttaaaagat tcccactatc ttaagtatta tagcaatatc taactgtttt tcctatgaaa
    49141 tcattgtttt tttatttaaa aactgaataa ttttttcttt ttttaatttt taaataaaag
    49201 aaatataaat aaaaacagag gaagcggaat gatccgcccc tctgttttta tgattttatt
    49261 aaacttccaa taattctttt tctttatcag cagcgatctt atctacttct ttgatgctgt
    49321 tatctgtcaa tgtttgtacg tctttttcta atccgcgcaa atcatcttcg gtaatatcac
    49381 catttttttg ttgtttttta tattcatcga tcgcatcacg acggatgtta cgaatcgcga
    49441 tttttgcatt ttctgcttct tttttaacat ctttagctaa ttcttttctg cgttcttctg
    49501 tcagttgcgg gataactaag cgaatgacat ttccatcatt ggttggactg attccgatat
    49561 cacttgccat aatagctttt tcaacatctt gaagaatgct cttgtcaaaa ggtgtgatca
    49621 ttaaaacacg tgcttctgga atttggattg atgccatttg gttcaatggt gttggcgcac
    49681 cgtagtagtt cacagtaata cggtccaaaa ggctagcgtt cgcacggcct gcgcggattt
    49741 gcccaagttc acgttgaaga ttttgtgctg cttttttcat tttttctttt gcttctgtca
    49801 taattgcatc tgccattttt atttccccct tacagttgtt ccgatatttt cgcctaagat
    49861 tgcacggcga atatttccgt gttcatttaa attgaagaca agcagtggaa tatcattatc
    49921 catgcttagt gaacttgcag ttgaatccat gacttgtaat cctttggcaa taacttccag
    49981 atgagtcagt tcttcaaatt ttactgcatt tgcatctact ttcggatcag cagaataaac
    50041 accgtctaca ttgtttttag ccatcaggat aacatctgcg ccgatttctg ccgcgcgtaa
    50101 tgctgctgtt gtgtctgtcg agaagtacgg gttgccggta ccgcctgcaa agataacgat
    50161 ccgtcctttt tctaaatgac gttctgcttt acggcgaata tatggctctg cgatttgacg
    50221 catttcaatc gaagtttgca cgcgtgtcgg tacacccacg ttttccaacg tatcctgtaa
    50281 agctaaggca ttcataactg tagctaacat acccatgtaa tcagcttgag cacgttccat
    50341 gcccatttgt gctccgattt gtccgcgcca gatatttccg ccgcccacaa cgattgccat
    50401 ttcaacgccc agctcgtgaa cttctttgat ttcttggatg atttctttga ttactggtgg
    50461 tttgattcca aatcctgcgt ccccagctaa agcttctcca ctcaatttta agactacacg
    50521 ctgatatttc ggtgtgacca tgttcattcc tccaatattt tctattgcca ttttaccata
    50581 cttagatgat ttcgacactc taagactggt ttttcttgtt ttgcttaaaa aaagggagcg
    50641 cacaaaccgt gcgttcccgt ctgttattca acagaattat tttttcactt ggctcataac
    50701 ttcttctaca aagttatctt cgcgtttttc gataccttcg ccaacttcga aacgtacgaa
    50761 tgttttaaca gttgcgcctt ttgatgcaac gtatttttca acagtcatat caggatcttt
    50821 aacgaatggt tggtccacca aagcgatttc tgctttgaat ttcttcaagc gtccttctac
    50881 cattttttca acgatattcg ctggtttgcc ttcgttcaat gcttgttctg taagaacagt
    50941 tttttcatgt tctaattcag cttctggaat ttgtgattcg tttacataac gaggattgat
    51001 agctgctacg tgcatagcta cgtctcttgc aactgtttcg tcagttgttc catctagaac
    51061 agtcaatacg gcaatacgtc cgcccatatg caagtatcca ccaaatgcag cattgtcgtc
    51121 tttttctaca acttcaaagc gacggaagct gattttttct ccgataactt gagttgcttc
    51181 gatcaagtca gattcaattg tacctttatc tgttttgatt ttcattgcag cttccatgtc
    51241 tgctggtttg ttttcagcaa caagttctgc aatttctttt actaaatctt ggaacatttc
    51301 gtttttagaa acaaagtctg tttctgagtt tacttcaacg attgctgcag tgttaccttt
    51361 cacagcaact gaagccaatc cttcagctgc aatgcggtca tttttcttcg cagcctttgc
    51421 cattcctttt tcacgtaaaa gatcaactgc tttttccatg tcgccttcta cttcaactaa
    51481 tgcttttttc gcatccatca ttcctacgcc agtcatttcg cgtagttctt ttaccatttt
    51541 agctgtaacg tctgccatgt ttaactcctc ctgtgtgttt ttctttttaa aaaaagctgc
    51601 ttcaaaggag agtgaggctt gtcgcccagc ctttgaaaca gctccatcat tcaaaattat
    51661 tctgctgaag aattgtcgcc ttcaacaaca tctacgattt cttcgattga tgttgctgtg
    51721 ttttcttctg tgaaatcttc ttcaactact tgatcttcac cttggtttcc ttcgatgaaa
    51781 gcatcagcca tttttgaagt aatcaattta acggcacgaa ttgcatcgtc gtttgaaggg
    51841 attactacgt cgatttcatc tggatcgcag tttgtatcaa ccatagctac gattgggata
    51901 tttaatttgt gtgcttcttg aacagcaata cgttctttac gtgggtcaac gatgtacatt
    51961 acatctggga ttcttggcat atcagcgata ccgcctaaga atttttcaag acgttcacgt
    52021 tctttgttca aaccagcaac ttctttttta ggtagaactt caaaagttcc atcttcttcc
    52081 attgcattga tttgtttcaa acggctgata cgtttttgga tagtatccca gtttgtcaat
    52141 gttccgccta accaacgatg gttaacgaag tattgtcctg cacgagttgc ttcttctttg
    52201 attgcttctt gtgcttgttt ttttgtacct acgaataatg ctacgccgcc ttcttcagca
    52261 acgctcttca tgtaatcata agctgcatct actaatttaa ctgttttttg caagtcaatg
    52321 atgtagattc cgtttctttc tgtgaagata tatttcttca tttttggatt ccagcgacga
    52381 gtttggtgac caaagtgtac gccggcttct agtaattgtt tcatagaaat tactgccatg
    52441 tttgtttcct ccgatttggt tttatttttc ctcttcttat cttctacctt gccggcaaac
    52501 tttttcaagc acctaccgac aatccaataa gaatgtggat ttgctgatgc ataaagcacc
    52561 ctcagctatt atacagacaa aagatccgta tcgcaagagc aaatatactt ttctgctcaa
    52621 cattcaatag tttacctgaa agataaaaag aattcaagaa aaacagtaga tttctctttg
    52681 tttctttatt atcctttttc cataaaataa ttttaggtat aaaagacgaa tattattggg
    52741 caatccttct tttttttgtt atgatacatg tgaaatcttt cgtaaaggag aaagaaaatg
    52801 cgcaaagctc aaagacattt gctgattaaa caagtcattg aagaattccc gatccgtacg
    52861 caagaagaac tattaaacaa acttgggcat tatggcgtta ccgccacgca ggcaacgatt
    52921 tctcgagata tccgtgatct aaaaatcgtg aaggcacctg atgaaaatgg cgtttcccgc
    52981 ttcgtattgt ttcgcgaaaa aggaactcct gagactagga ctgaggaaga ggatcggctg
    53041 gttcaaatga ttgaagacat tgtattgaaa gtcgaacgag tccacttttt gacgatcgtc
    53101 aataccttgc cagacaatgc tcatttattt gcatctgttt tagatgagat caaacctccc
    53161 tctattgtca gtacaattgc tggattcgat acgatcatgg tcatctcgga aaatgaggag
    53221 gacgcaaaac gagtagaaga atttcttcac catccaactg aagtatcact tttttaatca
    53281 gagcacgtat tcctaatatg gaatgcgtgt ttttttattt tcaatatttt ccttattgaa
    53341 taaaccttta cactctttac aaaaatacat cattttttat tcaaattttt caacaccttg
    53401 attgaatcac aataatcatc atttatattg gatgttaaca agagcagctt cttgctcttt
    53461 gtgtatttca aaaaagaaat gaaaggaaat catatgtata aattattaac ttctggacca
    53521 ttgacgactt ctgaaacagt gaaaaatgct atgctgatcg atcattgtac ttgggatcaa
    53581 gaatacaaag aaattacaca atggataaga aaaaaattat tggatcttgc tcaagtatcg
    53641 gaaggaatat acacaacggt cttaatgcaa ggtagcagct ccttcggggt agaatcggta
    53701 ttaagttctg tgatcaggcc agaagatact gtactgattt gttcaaacgg cgcatataga
    53761 caacgaatga tcaacatgtg cgaatgtctg aaactgaact ataccgttta tgaggtatcg
    53821 gaacatcaaa tcctccaagc tcatgtgatt acacagctga ttgaagagaa tccgtctatc
    53881 tccgcactag caatggtcca tagcgagact acttccggta ttttgaataa tttggaagag
    53941 attgctgctg tcacacaaga aagagatctt ttgtttatag tggacgcgat gtcaagcttt
    54001 ggtgggatac cgatttgtgt agccgaacta gggattgatt ttttgatttc cagctcaaac
    54061 aaatgtattc aaggtgttcc aggcttttct tttgtacttg ctaataaagc ggaactggaa
    54121 aaacgaaaaa atgttagccg aagtttgtca cttgatctat atgaccagta ccgcgtgatg
    54181 gaaaacgaga atggcaaatg gcgtttcact tcccctaccc atacggtttt ggcttttcaa
    54241 caagcgctga aggaactaga ggcagaagga ggaatcgaaa ctcgatattc acgttatctc
    54301 gccaataatc atgtgctacg acaaaaaatg acagagatgg ggttccagcc tttcattcaa
    54361 gaatatcagg ggccattcat cacctctttt ctttacccta aggactggcg atttgatttt
    54421 aatcattttt atcagtattt aaagcaaaat ggatttgtga tctatcccgg gaaaatcagc
    54481 gatcatgaat gttttcgtat cggaaatatt ggcgagatct atccggaaga tattttggaa
    54541 ctgacagcaa ttatcaaaga ctataaggag aacgttaatg attaaaactg taatttttga
    54601 ttgggcagga acaactgtgg actttggctg tatggctcct gtccacgctt ttagaaatgc
    54661 ttttctagaa aaaggaatcc aactgactga caaagaaatc cgctaaccga tgggaaaatt
    54721 aaaatgggat catatccaac agcttctttc cctcccttct gtcaaaaaac aatggatagc
    54781 tatttatgga caactacctc aggaaaaaga ggtggaggaa ttgtatcaac aatttgaaca
    54841 ttatctgttt aaagatttgg tcaagcattc taccttgaaa cccgacacat tggaaacgat
    54901 cgaacggttg agaaacgctg ggatcaatat tggttccacg aatggattta atgctcctat
    54961 gatgacaatc gtagcagaaa ccgcaaaaaa agaaaggtat tcaccagact tcatggcaac
    55021 ttttgaagac gtcgaagggt atggtcgacc gtttccttat atgatccata aaaacatgca
    55081 gtacttcaaa aacaagtcgg ttgatgaagt aatcaaagtc ggagatacgg tctcagatat
    55141 ccaggaaggg aaaaatgccg gagtgttgac agtaggtgta attgaaggta gttcgctaat
    55201 ggggatttct tatgatgagt accaaaaatt agagatgggg aaaaagatcg tttaaaacag
    55261 tctgttgctc ttagattcca acaagccggc gctgatattt gtgtaaacaa tttaaaagag
    55321 ttaacagaat acattttgta cgctgacatg aatccagaat gacttttata caaaataaga
    55381 ggttgtgaaa aagctgtcct gtatcaagta ataaaaacag ccaagcaaaa atagctcctc
    55441 atatttttcg tttggttagg cttattaccg cagatgcaag cttttgaaca ccgtttattc
    55501 gaaaaaaaaa agggagctgt gacacgactt ttgtcacagt ccccttattt gcaaacaaac
    55561 agtgtttaag agacaacctc tccactaaac ggtgttcatt cagctgactc tcggtatttg
    55621 ctcaaaaaac gataaaattt gaaaagctat tttctctttt gtttctctaa tactcggatc
    55681 agaacgctga tttcacattc tctttttctt acatcttatc tggtgcatga agtcctagca
    55741 aacgaaggtc ttctttcaat aatacagtca ctgcataaac tagagctaag cgtgcttctt
    55801 tttgttcatc atcagctaaa atcttcgtat gagcatagta tttgttgaat gcttgtgcta
    55861 atttgattgc atgtttcgca atcactgacg gttcgtattt ttccccagca gacaagaccg
    55921 tttcagggta tttttgaact aatttaacca cttcccagct ggtatcgtca ttcaatgcat
    55981 agtctgctgt ttctgaaggg acaaatcctg ctttttccaa caggctgact gcacgagcat
    56041 gtgtatattg cacataaggt ccagtttctc cttcaaatcg aacaacttct tccaggttaa
    56101 agtcaaatgt attcagtcga tcgtttttca aatcgtggaa aatgactgcc ccaaccccaa
    56161 cttgtttagc tacagcatct ttgttttcca aatcagggtt tttctcgctg atttgttctt
    56221 tagcagattg gattgcttca tttaatactt cttctaataa gacgatcttt cctttacgag
    56281 ttgataattt tttaccacct tgcgtgatca aaccaaatgg aatatggtgc atgtcgtcag
    56341 accagtcaaa gcccatttcc ttcaagacag ctttcaactg tttaaaatga tagctttgtt
    56401 cattccctac tacatataga gattgtttga agtcgtaagt acgtttacgg tacaatgcag
    56461 cagctaagtc acgtgtaatg tatagtgtcg caccgtctga cttcttaatc aacgctggat
    56521 tcaggtcata agcagatagg tcaacgatct ctgcaccttt atcctcgttc agcaaatgtt
    56581 tttcttctaa aagtttgacg atttcatcca tcttgtcatt gtagaaagct tcaccgttca
    56641 acgaatcgaa acgtacttcc agcagatcat agattttatt gaattctttc atcgattcat
    56701 cacggaacca ttgccataat tgaatagcct ctttatcgcc ttcttccaga cgtttgaacc
    56761 aagcacgagc ttcttcattt aattctggtt ctgtttcagc tacttcatgg aattggacgt
    56821 ataaacgaag caattcattg attggttctg ctttaacagc ttcttctgta ccccattttt
    56881 tgtaagcgac gatcagtttt ccaaactgtg ttccccagtc acctaaatgg ttgatgcgga
    56941 ttggctgata accgattttt tccataatga aaccgatcga attaccgata actgtcgaac
    57001 gcaaatgtcc cattgagatt ggtttggcaa tattaggaga agacatgtcg attggtactg
    57061 ttccttgatt cccgatgttg ctatccccat aatgttcttt ttctttcact actgtttgca
    57121 aaactttttt gctgataagt tctttattca tgaaaaagtt caagtaaggt ccgacaactt
    57181 cgattttttc aaagttggca gaatcgattt tttctgctaa atctgcagcg atttgctgtg
    57241 gcgcttttcg gtaaactttt gctaatgaaa aggctggaaa tgcaacatct ccgtgttcag
    57301 ctgattttgg attttctagt aattgctcga cttgttccag tgttaaatca tctttgacag
    57361 cgtcatagac agcttttgcg acaagatctt tattattcat ctttattcct cctaaagtgt
    57421 tcccttgatt gttaaacaaa aaaaatctct agcgacggca tacttagtat gccatcacta
    57481 gagacgagat ttcccgcggt accactccaa ttgtcctaaa aaaggaccag ctccatctga
    57541 tatcggtaga cgaccgtctg tttaaaacag aattctccaa gtgcgcttcg ttgtatcttc
    57601 ttcattcggc ttccaccact tccgaattcg ctagccaaga atgatacaag tactctcttg
    57661 ttcaacaaat cttagtctta ttaaactttc ttgaattata tcagaaaaaa aaacgaaagg
    57721 ctagtcgttt cataaaaaaa caatccagat ggcctaaagg agaagaaata ccacctggat
    57781 agctataaat catttcgtaa atacgaaaga cttaacaaat taatcagcta cgataatcgt
    57841 tccttcttca gaagctaaaa gattctcgat attttctaaa gaagtgatga ttgccttagc
    57901 gtttggtttt gcttcaacaa atgcaatagc tgcttctact ttcggaagca tgcttcctgg
    57961 agcgaattgc ttttcatcaa tgtattgttt catttctgaa acagtaacag tttctaattt
    58021 cttttgatct ggtttttggt aattgacata cacattgtct actcctgtca atacgatcag
    58081 caaatccgca tcgatgattt cggctaattt ttctgaagca aaatctttat cgattacggc
    58141 ttctcgtcct tctaatccag tagctgtttc tacaacagga atcccaccgc caccaacaga
    58201 gactgtgata actccttgat cgaccagcgt ttcgatgaca cgtgcttctt taatcgagat
    58261 gggtttaggg gaagcgacta cttttctcca tccacgtcca gcatcttctt tgaatgtcac
    58321 agtggagtca gcattcattt gttctttggc ttcttcttcc gtgtagaatg gaccgactgg
    58381 tttacttgga tttttgaatg aaggatcatt ttcatccaca ataacttgag tgactaaaga
    58441 aaccacatct ttatcgatcc ctttttcctt cagtacttcg cccatggcgt tttgtaacca
    58501 gtaaccgata gatccttctg tcattgcaac acatgtatct aaaggcattg ccggtgtttt
    58561 ctcagaatca gcagcttgct gctgaatcaa taggttaccg acttgcggac catttccgtg
    58621 ggagatgatc aattcatctc cttgttcgat aaatttcact aaatactttg ctgtttccat
    58681 taacgcttct tgttgggctt ttgcacttgc atccgtagac aagatggcat tgccccctaa
    58741 agcgacaact acttttcgtt tggccatttc attcgtcctt tcttcagttc taccgaattt
    58801 tctagataac aaaacgagcc agcgttttgt gattttccct caatgctgac tcgttttttc
    58861 gtgagatgaa tgtggttatg cttaaacgcg agggataaac aagttaccta atgttgctgc
    58921 catgatcgct ttgattgagt gcatacgatt ttctgcttgg tcaaattgac gagcatattt
    58981 gctgcggaag acttcatctg ttacttccat ttcagtgatg ccaaagcgtt ctttcatttg
    59041 ttcaccgtaa acagtgtttg tatcatggaa agctggcaag cagtgcaaga agatcaaacg
    59101 atctgtgttg tgtgtttttt ctaccatttc catattgatt tggtaaggtt tcaacaattt
    59161 gatacgttct tcgaatttat cttcttcacc cattgatacc catacatcag aataaaggac
    59221 gtctgctccg tctacacctt tatcaacatc gtcggtaatc atcaattgtg caccggattt
    59281 ttcagcaaag ccttctgcca ttttgacgat ttcttcttca ggttgtaatt cttttggtgc
    59341 aacgatacgc atattcgctc ctaaaattgc acctgtgact aataaagagt tagccatatt
    59401 gtttcgacca tctccgcagt aagcaacagt gataccttct actgttccaa agttttcttg
    59461 gatagttaaa aagtcagcga tcatttgtgt tggatgccat tcatcagtca agccattcca
    59521 aactggaaca ccagagaatt ctgctagttc ctctaccatt ttttgactga atccacggaa
    59581 ttcgattccg tcaaacatac gtcctagtac tttagctgta tcttctgtgg attctttttt
    59641 acctaattgg atatcatttg cacctagata ttcaggatgt gcacctagat cgatcgctgc
    59701 agtagtaaat gcagaacgag tacgtgtaga tgttttttca aataaaagcg caatattttt
    59761 tccttctaga tagtgatgtg ggataccacg ttttttcaag tctttcaagt gttctgaaaa
    59821 atcgatcaaa tattgtaatt cttcttttgt aaagtctttt tctgctaata agcttcttcc
    59881 ttggaatact gattctttca tattaaacat tctcctttag attgttttta tttaagatta
    59941 aagaggaaat cccgacggca gctgacagta agactgccgt caggaaacaa attacttcaa
    60001 gtcttcacgg acaagtggtt ggctcatgca gcgtggacca ccacgacctc gtgacaattc
    60061 acttgaattg atttctaaca ctttgatgcc atagctacga agcaattcgt tcgatacata
    60121 atttcggtta taagtgacta caacacctgg agcgattgcc aatgtgtttg atccatcatt
    60181 ccattgttct cgaggtgcaa cgatctcatc tccattacca gttgggatca gtacaagatc
    60241 atctaatcca agagcagctt tcatcgtttc gtgtaagtta tctgaatgag taatcttgat
    60301 atcatcacca tctggttcga ttgtaaagac atccatctta ccgtctttac tttgaattgc
    60361 tggatggata gtgaatttat cgtgatctac cattgtaaat acggtatcca aatgcatcat
    60421 tgcacggttg ttcggaattt tgattgccaa cactttttca aatcctgaat tttttgcgaa
    60481 taggtggcgt gctaattttt ctaaagcttt cgcattagtt cgttgagaaa tcccaacagc
    60541 tactacttta tcgcttaaga tcaattcgtc tccgccttcg atatgatctg gattttcacg
    60601 atccaaccat acttcgattc ctttatttgc aaatcttgga tgatgtttca aaatatattc
    60661 tgtaaacatc gattcacgac gacgtgcttc aaaagtcatc ttattgactg tcatcccatt
    60721 tcccattgat gcggaagggt cgcgtgtaaa gtacaagttt ggcattgggt ccatgtaaaa
    60781 tggatagtca tcgtctgctg acagatcata tagactgcta gaacgtactt cgatatcttt
    60841 tgtacgaacc ccagccatga ttttatctac catttcttgc gtttccattg atagaaggaa
    60901 ctcatgaaga ccctctctta cagcattcga tgcaatatga gattcatcca acattttatt
    60961 caagaattgt tctttgacat tgcctgcgtc aatcgcttct gccgctaatt tttccaagta
    61021 aagagtctcg acgccttcat tttgcaatgt cttcgcaaag ttgtcatgtt cttcttgagc
    61081 aatcggaaga tatggaatat catcgaacaa cagacgatcc ataatatctg gtgtcaaatt
    61141 ttccacttct tgtccaggcc gtttcagtaa aactgtcttt aatttcccta tttcagagaa
    61201 aacgtgaata ggtttatcca tgttgtgttg cctccttctt tttgattaca tgtataggat
    61261 atcctccttt tgtaatcact ttcataattt tgtctcattc aaaatgagac attcctcaca
    61321 ttaatattcg ataattaaat aattaaacgt ttttc
//
LOCUS       DAJSBL010000013        60464 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.013, whole genome shotgun sequence.
ACCESSION   DAJSBL010000013 DAJSBL010000000
VERSION     DAJSBL010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 60464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60464
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.013"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(12..1535)
                     /locus_tag="ONT85_000914"
     CDS             complement(12..1535)
                     /locus_tag="ONT85_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HCR2377908.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKDGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            1787..2968
                     /locus_tag="ONT85_000915"
     CDS             1787..2968
                     /locus_tag="ONT85_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCR2377909.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            complement(3028..4656)
                     /gene="treC"
                     /locus_tag="ONT85_000916"
     CDS             complement(3028..4656)
                     /gene="treC"
                     /locus_tag="ONT85_000916"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HCR2377910.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGENMSEGNGWNALFFNNHDQPRALNRFVVIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(5053..7764)
                     /locus_tag="ONT85_000917"
     CDS             complement(5053..7764)
                     /locus_tag="ONT85_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR2377911.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLPQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            complement(7774..8475)
                     /locus_tag="ONT85_000918"
     CDS             complement(7774..8475)
                     /locus_tag="ONT85_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR2377912.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            8661..9260
                     /locus_tag="ONT85_000919"
     CDS             8661..9260
                     /locus_tag="ONT85_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HCR2377913.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTIKKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            9341..10354
                     /locus_tag="ONT85_000920"
     CDS             9341..10354
                     /locus_tag="ONT85_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HCR2377914.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            10462..11379
                     /gene="rbsK"
                     /locus_tag="ONT85_000921"
     CDS             10462..11379
                     /gene="rbsK"
                     /locus_tag="ONT85_000921"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HCR2377915.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            11407..11802
                     /gene="rbsD"
                     /locus_tag="ONT85_000922"
     CDS             11407..11802
                     /gene="rbsD"
                     /locus_tag="ONT85_000922"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HCR2377916.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            11802..13175
                     /locus_tag="ONT85_000923"
     CDS             11802..13175
                     /locus_tag="ONT85_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCR2377917.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            13532..15676
                     /locus_tag="ONT85_000924"
     CDS             13532..15676
                     /locus_tag="ONT85_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HCR2377918.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(15728..16768)
                     /locus_tag="ONT85_000925"
     CDS             complement(15728..16768)
                     /locus_tag="ONT85_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HCR2377919.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMGNGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(17045..18847)
                     /locus_tag="ONT85_000926"
     CDS             complement(17045..18847)
                     /locus_tag="ONT85_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR2377920.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIILGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            complement(18861..19613)
                     /locus_tag="ONT85_000927"
     CDS             complement(18861..19613)
                     /locus_tag="ONT85_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR2377921.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            complement(19622..19984)
                     /locus_tag="ONT85_000928"
     CDS             complement(19622..19984)
                     /locus_tag="ONT85_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HCR2377922.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            complement(20160..21032)
                     /locus_tag="ONT85_000929"
     CDS             complement(20160..21032)
                     /locus_tag="ONT85_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HCR2377923.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            complement(21058..21570)
                     /locus_tag="ONT85_000930"
     CDS             complement(21058..21570)
                     /locus_tag="ONT85_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HCR2377924.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQARGWYITEDKELELEGVMARIRK
                     KIKKVTLDIY"
     gene            complement(21708..22499)
                     /locus_tag="ONT85_000931"
     CDS             complement(21708..22499)
                     /locus_tag="ONT85_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HCR2377925.1"
                     /translation="MLTGGLLAGATYTLGAQKSLTWDNGPRLIDMVTETKKIKDANKI
                     VIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKKSTGAIISLEDTSA
                     QITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSFNEFTGGTITAKDE
                     LGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSLHEMENSGYTIQTS
                     GESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD"
     gene            complement(22554..23162)
                     /locus_tag="ONT85_000932"
     CDS             complement(22554..23162)
                     /locus_tag="ONT85_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HCR2377926.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            complement(23155..23472)
                     /locus_tag="ONT85_000933"
     CDS             complement(23155..23472)
                     /locus_tag="ONT85_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HCR2377927.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            complement(23628..24356)
                     /locus_tag="ONT85_000934"
     CDS             complement(23628..24356)
                     /locus_tag="ONT85_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HCR2377928.1"
                     /translation="MPTGVLINVGSVLLGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            24515..25396
                     /locus_tag="ONT85_000935"
     CDS             24515..25396
                     /locus_tag="ONT85_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR2377929.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARNFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            complement(25633..26676)
                     /locus_tag="ONT85_000936"
     CDS             complement(25633..26676)
                     /locus_tag="ONT85_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HCR2377930.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLYFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFLQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIG
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(26679..27347)
                     /locus_tag="ONT85_000937"
     CDS             complement(26679..27347)
                     /locus_tag="ONT85_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR2377931.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            27489..29825
                     /locus_tag="ONT85_000938"
     CDS             27489..29825
                     /locus_tag="ONT85_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR2377932.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTDHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            complement(29940..30251)
                     /locus_tag="ONT85_000939"
     CDS             complement(29940..30251)
                     /locus_tag="ONT85_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2377933.1"
                     /translation="MKKMKESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            30432..31907
                     /locus_tag="ONT85_000940"
     CDS             30432..31907
                     /locus_tag="ONT85_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HCR2377934.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGMSAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            31954..32538
                     /locus_tag="ONT85_000941"
     CDS             31954..32538
                     /locus_tag="ONT85_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HCR2377935.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFGIVNEHSKRWQGD"
     gene            32540..32959
                     /locus_tag="ONT85_000942"
     CDS             32540..32959
                     /locus_tag="ONT85_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HCR2377936.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKTL"
     gene            complement(33240..33524)
                     /locus_tag="ONT85_000943"
     CDS             complement(33240..33524)
                     /locus_tag="ONT85_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HCR2377937.1"
                     /translation="MREIYSFESKIYASEVDKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNMPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(33876..34586)
                     /locus_tag="ONT85_000944"
     CDS             complement(33876..34586)
                     /locus_tag="ONT85_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HCR2377938.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            complement(34583..36055)
                     /locus_tag="ONT85_000945"
     CDS             complement(34583..36055)
                     /locus_tag="ONT85_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HCR2377939.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            36193..36666
                     /locus_tag="ONT85_000946"
     CDS             36193..36666
                     /locus_tag="ONT85_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HCR2377940.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            complement(36819..37370)
                     /locus_tag="ONT85_000947"
     CDS             complement(36819..37370)
                     /locus_tag="ONT85_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HCR2377941.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            37475..38005
                     /locus_tag="ONT85_000948"
     CDS             37475..38005
                     /locus_tag="ONT85_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HCR2377942.1"
                     /translation="MKEFKELEDFIGTRFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            complement(38488..38931)
                     /locus_tag="ONT85_000949"
     CDS             complement(38488..38931)
                     /locus_tag="ONT85_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR2377943.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(38947..40107)
                     /locus_tag="ONT85_000950"
     CDS             complement(38947..40107)
                     /locus_tag="ONT85_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HCR2377944.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKKQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            40614..42314
                     /locus_tag="ONT85_000951"
     CDS             40614..42314
                     /locus_tag="ONT85_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2377945.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPSFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQKKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            complement(42416..43249)
                     /locus_tag="ONT85_000952"
     CDS             complement(42416..43249)
                     /locus_tag="ONT85_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HCR2377946.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCQVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQSQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(43553..45718)
                     /locus_tag="ONT85_000953"
     CDS             complement(43553..45718)
                     /locus_tag="ONT85_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCR2377947.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            45978..48272
                     /locus_tag="ONT85_000954"
     CDS             45978..48272
                     /locus_tag="ONT85_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HCR2377948.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGFPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            48265..48930
                     /gene="pgmB"
                     /locus_tag="ONT85_000955"
     CDS             48265..48930
                     /gene="pgmB"
                     /locus_tag="ONT85_000955"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HCR2377949.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKCH
                     DHG"
     gene            48923..49930
                     /locus_tag="ONT85_000956"
     CDS             48923..49930
                     /locus_tag="ONT85_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HCR2377950.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            49952..50971
                     /locus_tag="ONT85_000957"
     CDS             49952..50971
                     /locus_tag="ONT85_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR2377951.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDPVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(51252..52619)
                     /gene="nhaC"
                     /locus_tag="ONT85_000958"
     CDS             complement(51252..52619)
                     /gene="nhaC"
                     /locus_tag="ONT85_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HCR2377952.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFVFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(52729..54150)
                     /locus_tag="ONT85_000959"
     CDS             complement(52729..54150)
                     /locus_tag="ONT85_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR2377953.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(54393..56270)
                     /gene="tdc"
                     /locus_tag="ONT85_000960"
     CDS             complement(54393..56270)
                     /gene="tdc"
                     /locus_tag="ONT85_000960"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HCR2377954.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(56561..57817)
                     /locus_tag="ONT85_000961"
     CDS             complement(56561..57817)
                     /locus_tag="ONT85_000961"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HCR2377955.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEVEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     misc_binding    complement(57929..58134)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            58397..59698
                     /locus_tag="ONT85_000962"
     CDS             58397..59698
                     /locus_tag="ONT85_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HCR2377956.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(59767..60366)
                     /locus_tag="ONT85_000963"
     CDS             complement(59767..60366)
                     /locus_tag="ONT85_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCR2377957.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
BASE COUNT        18241 a        12369 c        10836 g        19018 t
ORIGIN      
        1 ttttttgcct atcagtgcaa aagcatttcc tgtgcaattt cacgtccatc cattgtatct
       61 ggataaaatg ttggccaatt ctccatttct ttcagcaata cttcttgact ttcatttcct
      121 gcatataaat gaaaatgttc ggagtcagcc ggtgcaatcg tgtgatcact gaattgaata
      181 tatctaaaag cgccactttc cttatcttct gcttcgaata gataccttac gccacggttt
      241 cctgcctcgt aggtcaagat ttctttccct acataacgat aatttgaacg tttcgttgtt
      301 ccatccttaa acgtaaatgc catctctttc tcactgatct tgattgaagc aatatctgtt
      361 tgatagccct tcgtataata ttctttatat tcttgtgctg tcttatcctg attcaatttt
      421 tctttatatt caaaaacagg atcaaaagtc ccatcttcta catacgtatc tactgtttgc
      481 caagttcctg cccaatctga aagagagcga tcttgtacat cctcgtcttc aaaataaccg
      541 ttgtagacgc tcttttcttt tttgggggtc aaagtatctt ctagcgggtt ttctgtctct
      601 gtcgtttttt tcaacgcttc gaggtttgct tccatgatag aaagatagtt ttctccattt
      661 tctatttgtt cattcgtcaa tccttccaaa ggattcaata cttctaaaga tacacctgct
      721 tcgtttgcta acgttctggc aatactatct ttggcgtttt cttcaaaata aatatagttg
      781 attccatgtt ctttaacaaa gtgtttcagt tcagcaattc tcgccgcaga cggttcttca
      841 ctagaagaaa gaccagcaat cgatacttga ttcaatccat attctaacgc tagatagttg
      901 aatgcagcat gttgcgtgac aaaagatctt tgttttgctt gcttaaaggt ttcttgataa
      961 agctgatgga gtgcttgaag tttctcggta taggctgctg cattttttgt ccatacttcc
     1021 tgtttttctg ggtaagcttc aatcagctga tcacgtatgt ttgctacctg cttgattgct
     1081 aaacttggag ctagccatac atgtgggtct agctcatgtg tatggccttc ttccccgtgt
     1141 tcatggtcat gatcatgtcc ttcttcactt cccggaagta aaagcatacc ttctgtcgct
     1201 tttatcgtat ggacattgcc ttcttgtaat gttttttgga ttgcggggac ccatgtttcc
     1261 atgttttcat cattataaac aaagatatct gtatcttgga tctttgccat gtctttagca
     1321 gaaggttcat aatcgtggga atcagtccct gctggaatca ataactctac ttctccttca
     1381 tccccgacga ttgcttttgt aaaatcatac atgggataaa aggtcgtcat cacgctcaat
     1441 ttttctttac tgtcctcggc tttcccactt gccccgcaag cagttaacat acctgctaaa
     1501 accagcatgg ccaatatacc tatcaatcgt ttcatttatg acactcctct tctcatcttc
     1561 tttcatttga atgttccgat tcgcttttcg caaaccgtaa tagttacgtt ttagcaacag
     1621 gatttaattt atcatatggt taaaagaact gtcaagcgtt ttcttatttt aattgatgag
     1681 aaagtttata tgaatgattg acgatatttt tgcaatcaac ctctatcatt ttccttttat
     1741 acccgctttt gtctataatg aagttataga taaaggagga aaaagagtga aaaaagaaac
     1801 agatacaacg aatcgtttga tccgcttttt aggaggaaaa acctcttact atgttttagg
     1861 aatagttatc ttacttgcgc tgatcattta ttttttccat cagatctcgt ttatattcaa
     1921 gcctgtcgga atcattatcg ctactatctt gcccccatta ttgtttgcac tgatcttgta
     1981 ttatcttttg gaaccagtca tcaaacgttt gagcaagcac ctttcgagga acttgtcagt
     2041 cattatcgtt tatgtgatta ttttattttt attggggctt ggaagcatat ggcttattcc
     2101 ttttcttgaa gaacaggcaa agacattgat cagtagtttg ccagatttgt ttcaagattt
     2161 tcaagagagt ctccgacaat ttttggagaa aacacctttt gccgattcat ttaatcaatt
     2221 ttttgcttct attgacgacg tgacgtctga tatagctgga tttattggaa attattggga
     2281 aagtggcgca caacggatca gcaacatttt ttcaactgtt acagctattt ttattacttt
     2341 atttactggt ccaattgtcg cttttttcct tttgagagat cctcaaaaat tttatcagtc
     2401 agttttagcg attgtcccac cagcatttcg tacagatttt aaaaatttga caaagattgc
     2461 taatcaacaa ttgggttcat ttctaaaggg gcaaatcatt gcttcattta tccttggagc
     2521 agtctactgg gtttgtttct tactgatcgg attagaattt gctagtgttt tagctatttc
     2581 tgctgggttg ttatgtatca ttccatatat tgggccgttt atcgccttct ttccaggttt
     2641 gatcattgca ttccaagact ctacttttat ggctgtcaaa tttgtcattg tctggtttgc
     2701 cgtacaactt ctccacggtg acttggtgat tcctcgagta atgggtgatc gtttacagat
     2761 ccatccgatt acgatcttaa tcgttttact tgtcatgggt gatctaatgg gaattgtggg
     2821 cgttattttt ggtattccta tctatacgtt agtcaaatta ctcgttactt ttattttccg
     2881 taaattcaaa caacgctaca ataaatttta tggcgataaa ggagagtatg aaaactcttc
     2941 attttctgaa gaagattatt ttaaataaaa cacaaaacgt cgatcttacg agaatagctc
     3001 gtaggaacga cgttttgtgt ttttgtttta atttttcagc aaagcaattg cctggtatgg
     3061 ttttagctcc agactttgat gaagaacagt atcggaataa ttatcgataa gaatattggc
     3121 ttctgtgaat ccttctggga tggcgatcct tgtatcttta tcatagaaat ttgttaatac
     3181 aagcagctgc tgtccttcaa attggcgaag aaagccataa acttccggat gatttgcttc
     3241 ataagcatga taatctcctt gtgcaacgat tggatatgtt tttctcagcc gaatcaattt
     3301 cttgtaatag gacaaaatag aatcttcaga agatagttct ttttctacat taatctcttc
     3361 tagcttgcct ccaactttta accaaggttt ccctgtagta aatcctgctt gttctgtgtc
     3421 tgaccattgc atgggtgttc gagagttatc gcgagatttt gctttgatcc gacgaaacgc
     3481 ttctttttct gtgaatcctt gttcaaggag taacttataa gcattcaaac tttctacgtc
     3541 aatatagtcg gaaatcgatg aatagtctgg gtcgatcatg ccgatttctt ctcccatata
     3601 aatatagggc gtcccacggt tcaaatggat catggttgct agcatctctg ctccttgttt
     3661 tcgatactga tcgataacta caaatcgatt gagcgcacgt ggctgatcat gattattaaa
     3721 aaatagagca ttccagccgt taccctcact catattttct ccccatgtat gaaatagtcg
     3781 ctttaattct gcaaaatcaa atggaggagt agaccattta ttcccatttt cataatcgac
     3841 tttcaaatga tggaaattaa agaccattga taattcttct ctactaggtt ctgtatacaa
     3901 gatacaattt tcgatcgtcg tagagctcat ttcaccgact gtgatgatag tggaatccgt
     3961 cccaaacgtt gcttggttca attcttttag atacgtatgg gtgattggtt tgtccgtata
     4021 ttcaggtttg ccatcatttt ctttattatt tttcaattgt tcgtctttac cgataacatt
     4081 gataacatcg aaacggaatc cttttacccc tttttccatc caaaaacgaa caactttgaa
     4141 tagttcctct cttactgctt ggttccgcca gttcaagtca gcttgtgtcc gatcatataa
     4201 gtgcaaataa tatttccccg tatctccaaa tggcgaccaa gcacttccac caaattttga
     4261 tacccaatcc gttggctctt ctcttaaaat gaagaagtcc tgataatgct ggtcccctgc
     4321 tagtgctttt ttgaaccatt catgttctgt cgaaacgtga ttcagcacca tatcgagcat
     4381 gatttctatc cggtatttct ttgcttcggc tattagctgt tcgaattcct ccatcgtacc
     4441 aaagatagga tcgatggctg tgtaatcaga gatatcataa ccgttgtctt tttgagggct
     4501 tggataaata gggtttagcc aaatcatgtc gatgcctaga tcattcagat acgggagttt
     4561 ttctttgatt ccttttaaat ctccaattcc atcaccatta ctgtctttgt aagatttggg
     4621 atagatttgg taaatgactt tgtctttaaa tgtcatggat atttacctcc attcaactga
     4681 tttattacct accacagctt atatatacaa gttatcattt attattataa ctgaacagaa
     4741 agcgtttgta aaatttatct ttatacattc taagaggtct gttgcccaat aaaaaacaag
     4801 agatcagaca tgtatttgtc tatcctcttg tttaaaatag caaaatattc ttgtttcttc
     4861 tataaatggt gttcaaaagc ttttatctac ggcaatcagt cttctcaaat gtaaaacatg
     4921 aggagctaat tttatttgag tattcttttt gctcgatgca ggacggcttt tttacaacct
     4981 ctgattcatg gtgttcaatt agcagctgac cctcgttatt agctcaaaac gctgatttca
     5041 caacctctgt ttttattcat tcgatttcaa cgcatcaatc atatcgatat gctttaattt
     5101 gaaatgggta acaatcatca caataaccgt aaatacgata gttaatcctg ctgataagac
     5161 ataagcccac catgtgatca ctaatggaaa gatgacattc tccatagacg cttgttgcaa
     5221 gatgaagtca gtcaatatat atcctacacc aaatcctccg atgattccta aaagagtaaa
     5281 gatgatattt tctcgaacaa tgtacatcgt gacttcttta tcaaaaaatc caaggacttt
     5341 tattgtagat aattcgcgta cacgctcgga aatattgata ttagttagat tatacaagac
     5401 gataaaagcc agcagtcctg ataatacgac aaagattaag acaaccgagt ctaagttcgc
     5461 cattgattct tcttgcgttt cgatttgttt ggacatgaaa gacgtatttg tcacagcgtc
     5521 tgtggcaagc agtttctcgg aaagcatttc ttcttctttt gttgtcatct tatctgtacg
     5581 cagcaaaaat gcattatcca tcatcgattg atcagtaact gaagcataat attcagggga
     5641 catataaata aagctaccta gatagttttt cacgatttct tttacttcga ctttccattc
     5701 tttttgatct tcatcataaa gcgaaatatg atctcctgta gataagtcaa atagttctgc
     5761 tactttttgc gtaatagcga ttcctgtttt aggcaaggag cgtgggttct cttcaagatc
     5821 agttaaatgg ataaaacgat ccaaatcagc tgttttactt ggtacgatca ctgtcagact
     5881 ttgcgaagca tgtccttttt ttctcaattc tacggtttga gtatgaatag ctaggatctc
     5941 atttactttt tcttgcttct gcagcatatc cgctgctttt tgacgactat cttccgtgga
     6001 tgaatccaat gtaacgattg cttgatagtc aatgatcggt ccaaattgtt tggctgaaac
     6061 agaacttaga gaatctttca atcccacgcc tgcaaccatc agcccggcac atccagcaat
     6121 tccgataatc gccataagca ttcttgactt atatcggaag agatttcggt aactgatttt
     6181 ttgattaaag ctaaggcgtg accacagcgg agtgatgtat tcaagaaaaa tgcgtttccc
     6241 tggcttcggt gctttcggtt gaagcaatgc agcaggccgt tctcttaaat ctttgaataa
     6301 aacgatcatt gcagcgccaa atgcggcaat gaagaatgcg gcagctgctt gtaaaatcgg
     6361 cggccagtca taaaaaataa gcgcactccc aatatcatac cgctcgttag acagctcaaa
     6421 aatgatcctt ggcaaaagat tcgttcctaa tatcgttcct aaaacaatac ctatccctga
     6481 ggctagactt gcatagattg catatttttt tgcgatttca actttcgtat aacctaacgc
     6541 ctttaatgtc ccgatttccc tgcggttttc ttctaccata cgtgtcattg tcgtaaaggt
     6601 gatcaatgcg gcaataaaga aaaagaaaac aggaaaaaca ttggcaatcg cagcgatacg
     6661 atcagacaga tcaccaaatt cttgaaaacc gacattttct gaacgttcat tgtataggta
     6721 tgatggttta gttaaatttt caattttttc tgcatttcga ttcaattctt cctctgcaga
     6781 ggccagttca cgttctttct caactaattg cctttcttgt tcagcaagtt gagcttcttg
     6841 ttgaggtaat gtttgaacag cgattgttcg ttgcgcttca ggaagctgct ggatagttgc
     6901 tgactgttgc gctaactgtt ttttagcatc ttctaaccgt gttcttgctt gcgttgtttg
     6961 tgctttgcca tccgctattt tttgctgtgc cggttctaat tcttcgttgg ccgatttttt
     7021 cagctcttct aagcgttctt cgggtcgatc ttgtaaatac ttttctgcct tttcttgatt
     7081 ttgctccatt ttatctttgt aagctttgct gtacgtattc aaattcttta cgtttgtaaa
     7141 atcaagatat aagacagaat aaatatctgt tttaaaaacg gattcaggta aatagacaaa
     7201 ataatcgacg ctaccatttc cgacgttcgc gtatcctctc tccaaattgt tgatgaacaa
     7261 tggagaacgg acaaagcccg tgatggtata ctctttttct ttcaattcat ctgaatcaat
     7321 ctgataaata tcaccgattt tatacccttc atcttccgct ttttcatcca atacaagctg
     7381 gtttggtttt tccgggagtt ttccatcagt taattgtagc ttgttctgct tgttattttt
     7441 gttataagaa aatatttgaa tcacttcgtt tttgccagta ttggaataat agatctgttt
     7501 tcctgtttct acttttgcat ctgggatctt ttctgcttga gcaatatctt tttgtgtcaa
     7561 gccaccagta gaaacgattt gcaaatcaga aagtcctgct gtaccaacat agtgatccat
     7621 cgttttttgc atgacaggtc cagcagtttt gacacctaca aataaaagtg ttcctagcat
     7681 gacaataagg atgattgcaa tgaatcgtcc tttagattgc cgaatttcgc gaaaacctgc
     7741 tttaagatat gttcttgttt tcataaaatc cccctaccat acaatctcat caatggatga
     7801 aggatgatca tttaattcaa cagaccttac tttagcatca ttaatgtgaa tgactctatc
     7861 agcgatgggc gctaaagcag aattatgtgt aatgatcaaa acggtattgc cgtgttgtct
     7921 gctagcgttt tgcaataatt tcaatacttg cttgcccgtt tcaaaatcca gcgcgcctgt
     7981 cggttcatca cataaaagaa gtttgggatt tttagctagt gcacgagcaa ttgaaaccct
     8041 ctgctgttcg cctcctgata attgggacgg aaagttattc aatcgatgag ctaggcccac
     8101 ttgcctcaat acctctacag gatcaagcgc atcaggagaa acttccgttg ccaattccac
     8161 attttctttt gctgttagat taggaacgag attgtagaat tggaaaacga accctacatc
     8221 tgttcgtcgg taagcagtaa gctgtttatc tgaaaattgc gcaatatctg tgtcatcaat
     8281 aatgatctga ccttcatcag gtgtatccat cccacctaaa atattaagaa tcgttgactt
     8341 tcctgctcca cttggaccaa gtataatcac taattcgcct ttttcaattg aaaaagaaat
     8401 atcgtcattt gctgtaatgg tcgtttctcc cattttgtat cgtttgtatt cattttttac
     8461 ttcgacatac ttcactctga ttcctccatt ttcacgatat ttctcgctat atctatgcat
     8521 cgaatgcgtt ttctcacact tatttatttt accactgaat ttctctagac taaagaaagg
     8581 aatcctttaa tttagttgac aaataaacga tttatgattt acttacataa aaaacattgt
     8641 tatttgaaag aaggaacatc atgaatgatt taaaggaaaa tatgattttt gatttatcaa
     8701 aaactgcttt tgtcgcgatc gacatgcaaa aagagatcgt aaataacggt acactctctc
     8761 cacacactgc agcatctatt ctgacaaaca atgatttgtt agtaagaaca ttgaaacgta
     8821 ccgctgcctt gaaagtttta gtgaacgtag acatctccac tttcccttat ttgagtcaac
     8881 aaactgatat gggtggaatg actgcttctg tacctccaga attcactgat ttacttttga
     8941 aggattcttt gaaagataca gataatatgt tgactatcaa aaaatacaat ccaagtgctt
     9001 tttttggtac ttctcttgat cttcaacttc gccggcgtgg aatcgaaact atcattttaa
     9061 gcggtgtagc gacaacaaac ggtgtatatg ctactgcatt agatgccttt caacacgggt
     9121 accatatcgt gcttgctgaa gatgcttgca gtgatcgtga caaagaatcc catcaattgt
     9181 tcataaaaaa gatatttccg aaaactgcac gagtacgctc aacgaaacaa ataatcgaag
     9241 ctatccaaca aagcaaataa gtactggcac acgtgatttt catttacttc tttttgattt
     9301 catgggaaaa taaccacagg atttagatag gagtatgaac atggtgaaaa aaaagatcac
     9361 aatcaaagaa gtcgctaaac tttccggcgt ttcaatcaca actgtctcct tgatattaaa
     9421 tggaaatacg gagaaattca gtccgaaaac tgttcagaaa gttttagctg ccaaagaaga
     9481 attaggctat cagcctgact attttgctag acaaatgatt accaaggaaa cgaaaacaat
     9541 tggtgtactc gttcctgata tcacgaatcc attcttcagt acactgatgc gaggaattga
     9601 ggatatcttg tataagcaga atttcgtcac gatattatgt aatgctgatt ctgatcatca
     9661 aaaagaaatc gaatatctag ccgagctcac acgtcgaggt gtggatggat tcattatcgc
     9721 gacttctgct gtctctacag atgcaatcaa cgaaaatttg aaaaaacaag gccggccctt
     9781 cattgttttg gatcagaaaa aatctgaagg atttagtgat gccgtacgaa cagacgattt
     9841 tcgcggtggt tacctagcgg gcatgcactt gcttagtcta ggacatcaaa cgatcgccct
     9901 tgtctatcct gaaaatccac cggaaaatgt ccacgctcgg attgaggggt ttaaatctgc
     9961 tcttgatgtt taccaaatcc ctcacgacca actgatcctc ttacctacac agttctcaaa
    10021 acaaggtggc taccagatca cagcagaact tcttgattct gctgcaactg gtgtgtttgc
    10081 tttaaatgat gaattagctt ttggattata tcgcggatta gaggaagctg gaaaaagcat
    10141 ccctgaagac tacagcatta ttggctatga taatatagat atgtgcgagt atatcaagcc
    10201 gaaactgaca acgatcgcac aaccaatctt cgaattggga caaacatctg ccaagttatt
    10261 gttagatcgg atccaattcc cagaaaaaga atgggaagaa aaaagattac ctgtacgatt
    10321 agaaaaaaga ttttcaactg cacccttgaa atagctttca aggtgtgcta tatttttatc
    10381 gaactattaa aacgttttac taaaacgttt taaaaaataa ttttcccatc aaaataaatg
    10441 agatagttgg aggtttgtaa aatgaacacg gttacagtaa ttggaagtat taatatggac
    10501 actacccttc gtttaaccaa catgcccaag ccaggcgaaa cgatgcatgc gcatgagatt
    10561 tttcacgctg gcggaggtaa aggtgccaac caagcagtgg cagccaaacg ttcaggcgca
    10621 cggacatctt ttattggagg cgttggggct gacagcgaag gacaacagtt attagatcta
    10681 ctgacaaaag aaaacatcga tacatctggg atagctgaaa tccaaggcgc cacaaccgga
    10741 caagcaatga ttatggtaga cgcagcagga gaaaatagta tcttgatcca tgcaggggca
    10801 aataatgctt tccatgaaca agaagtccta aaaaacaagc aactgattac aaacagtgac
    10861 tttattattg ctcagtttga aagtagctta gatgcgacga tcctagcttt ttctattgca
    10921 aaagacgcag gaaaaacaac gatcctgaat cctgcaccag ctcgtgaaac gattcctact
    10981 gaactacttg aaaagacaga tatcattata ccaaatgaaa cagaaacaga aatcattaca
    11041 ggaatcagag tgacagatca taacagtttg gtcgcagcag ccgaaaaact ccatgaatta
    11101 ggtattggaa cagtaatcat tacactaggc agtgccggcg ctttctatca tacagaaaaa
    11161 gaacacggta tcgtccctgc ttttaaagtg gatgccgttg acacaacagc cgcaggtgat
    11221 acatttattg gcgcattaag cagcacactt cagccagacc ttagtaactt gaaagaagcg
    11281 atcctttatg gaaatctagc ctcttctgtt gctgtgcagt cttatggtgc gcaaccttct
    11341 atcccttacc gcgaagcttt agaacaatca gcagtctaaa attaacaatg agaggaagaa
    11401 acaaacatga aaaaaggaaa agtaatcaat tcagatattt cacgagtaat cgcacaaatg
    11461 gggcactttg ataaattaag tatcggggat gcaggcatgc ctgtcccctt tggtactgaa
    11521 aaaatagatt tggctgtctc aaacggtctt ccaagttttt tatctgtttt agaaaacgtt
    11581 ttagaagaac ttgaggtaca atcgattgaa ttggcggaag aaataaagac aatgaacccg
    11641 gaaatattgg aaaaaatcaa attattgtta ccagatacac cgattagctt tatcccacat
    11701 gaagaaatga aaaaagagtt gaataactgt catgcattta ttcgtacagg cgaaatgaca
    11761 ccttacgcca atattatatt agtgagtgga gtggtcttct aatggaaacg acaatgaatc
    11821 ctgaacagat ggtactgtcc gagcagattc atatgctgat tgaacagttg ggaggaaaag
    11881 acaatattga ttctgtcctt ccatgtgcga ctcggcttcg tatcaaattg aaaaaacagc
    11941 cagtagccaa tgcagaaaaa ttaaaacagc aaatcccttc cattaagtat ttcattttac
    12001 tcgatagata tgcagaaata atcatggatc agggattggc tcctgcttac aaggaattga
    12061 cgcgtcaaat cgatcattca gctccgaaag aaaagctaaa tccgatcgaa aaactatttg
    12121 gtttgatatc ggaaatcttt acgccgatca ttccgctgtt tgccggttct ggtatattaa
    12181 aaggtctggt tgttttagcg actacgattg gctggcttag tgaaacaagc ggaacttatc
    12241 atattttgtc tgctgcgtcg aatgccgtct tttattttct gccaatcttt cttgcttttt
    12301 ctgctgccaa aacgtttaaa gtgaatggat atattgcggc tgtcattgct gcagcactga
    12361 ttgaaccgaa tctgacctct ttactttcag acagtggcaa agcagctact gattttctgg
    12421 ggattcctgt agtactgatg cagtatacgt ctactgtcat gccggcgatt ttaggtattt
    12481 tcttttattc ttttgttgaa aaattcctca aaaaatatat tcgcgaaaat atgcaattag
    12541 tatttgtacc tttactttca ttagttatca cggttccttt gacgttaatg gtattcgggc
    12601 ctgcgggtgt ttaccttggc gaaggtttag ctgccgcaat tacttggatg atggatatta
    12661 acggaccgat cagtggcgca atcatcgctg gtggttggaa tattttggta atatttggta
    12721 tccaatgggc tgtaaaccct gtcatgatca gtaatatctc tgcttatggg ttcgaccgaa
    12781 tcgttccgtt gacaggagca gccaattttg gaatggctgg tgcagcttta ggtgtatttc
    12841 tgcgaagtaa acgttcaaaa acccgttcga tctccggttc tgcttttgct tcgattttac
    12901 ttgcgggagt gaccgaacca actgtttacg gaattgcgat cccattgaaa aaaccatttg
    12961 ttgctgcttg cattggtgca gctgctggtg gagctgtaat gggatttgcc caagtgaaag
    13021 cgatcgcttt tgtatttggt agtctgacca cattgcctgc attcatttcc ggtacttttt
    13081 tctggtactt agcaggactg gctgttagtc tagtcgtagc aatgatcact actctcgtta
    13141 gcgggtttga tgaagaccta atgtcttatg aatagttaat gaaaaagact gaagtatgag
    13201 atgtttatca catatttcag tctttttctt tttaggatca ttatttttat aaatttttgt
    13261 gatactttcc aaactttggt ataatttaca tatgcataac catttttatc cccacttgtt
    13321 tatccccaaa caaaggtggt ctatgcaata aaatgaacgt caaagaaaaa cgaatgaatg
    13381 ctcgttgctt atttacaaat tgttttattt agaatacaca gacagattta tgaaaagaaa
    13441 aagcttttcg acacgattat ctgttccaaa aaatgtctaa tgaatgctga ataagacaga
    13501 aaatcaatga aaggaaaagg actagtttat tatgaaaaat aaaaaaacga tacgtttgct
    13561 agctgctact tggctgattg ctccgtctgt tttgtcgacc aataccgtat ttgccgtaga
    13621 taatcaaata gctgatcaaa cagtcacaac agatacgact acatccagct cagctactag
    13681 tgaaacaacg gattctttta cagaagaccc aactggaaca actaactcaa caacaggatc
    13741 gaccgagcca actgaaattg atccggctga tccatcagaa aatgaaaagc cagccgagac
    13801 atattccctt acaattgctc cttctttgat tggtaagatc caactgactg cttcgacagc
    13861 ggattcccct gaacagcaac ctttggatat tacggaaagt ggaacggtag aaggtttatt
    13921 agcggaaacg aaggtaactt acactattac gccagctgaa ggtgaacaaa ttgcttcagt
    13981 cgctgttaat ggtgccaaag aagtaaatta tgatgcaaag acctttacag taggtacgaa
    14041 caatttatct attactgcag aatttaaagc tggaccggat acttcaacag agaaaccaac
    14101 tgatcctagt gaagaagtcc ctagtacacc agatccggaa acgccaaaca aaccagatgt
    14161 gggtaataac aacaataata atggttcaaa taattcaaac aataataaca ataattcgaa
    14221 cagcgggaat tcttcgaatc aaacaaaccc atctggatcg aataacaatg gaaatactca
    14281 gagagaacca aaccatccaa acaatggttc atcgaatggc ggaacgaaca atgcttcatc
    14341 tcgtccaaat acagcaaatc aaagcggtat agagaatcca tcttctgctt cttctgattt
    14401 tgttgtaaaa tcaccaattg atgctgtatt gcctacaaat acaacgaatg tccaacaagc
    14461 aatcgttcga gaagcattca agcatcttgg aaaaccatat gtatggggag caaaaggtcc
    14521 aaatactttt gactgttctg gtttgactta ttacgtatat atgaaagcaa ccggtcatta
    14581 tatcggtggc tggacaggcg aacagcaata tgcaggtacg cagattccag taagtcaggc
    14641 ccaacctggc gatctcgtct tctggggacc ttcttccggt gttactcacc atgtaggtat
    14701 ctatattggg aacggacaat ttatccatgc gcctcaacca ggagataaag ttcgagtaac
    14761 ttctatttca gatttcacgc ctgattttgc tgtccgagta aacttagcag gtttacctgc
    14821 tgcttcaggt tcgctgacag gcggctcatc tattttagat ggattagatg gctcgttcca
    14881 tttcagtcaa aaccaaacaa ctgatcagtt cttgaaaaag attgcagatg atgcacagga
    14941 aatcgggcaa aaagaaggaa tctacgcatc agtcatgatg gctcaagcta tccttgaaag
    15001 tggatctgga aacagcttat tatcaagcga accaaatcat aacctttttg gtatcaaagg
    15061 atcatataaa ggaagcagcg tgaccttcaa tacacttgaa caagatagtt ccggacaaag
    15121 ctaccaaatc cgcgcacaat tccgtaaata tccttcttac aaggaaagtt tggaagacta
    15181 tgccgacttg atcaaaaatg gactgactgg caatcctgat ttctacaaac caacttggaa
    15241 gtcggaaaca aaagactaca aagaagcaac caaatatttg gaaggacgtt atgctacaga
    15301 tcgccaatat tcacaaaaat tgaacgcgat catcgaagcg tacgacctga caaaatatga
    15361 tgaaccgaaa aaagaagcag caacaactga agaagcaaac gctgaaatcc tcaatatgtc
    15421 taagcgattc gatgtgccaa tcaaatggac aacagatggc gtgccaatca caggattaga
    15481 attttcaacg ataaaacgcg tcaacaacgc ttcttccatc ttccgtgtcg cttcaatctg
    15541 ggatttgtgg aacaatttta cggcaagaca gataccagaa acaactactc gtacagttaa
    15601 agctgcaact gttcctgtac taaccttgtt aagtttagac aaatcattct ggcttgatct
    15661 gcctttaaag aaatagaata aattaaaaag gggatgtgac actgtcacac tcccttttta
    15721 atttattcta tttctttata tttattttgt attccgttac aaactcgcgt acttggttag
    15781 gttccagagt gacatctcca aaactttcaa gtcgctccgc tcctggcagt tcttgagtct
    15841 caaatgtgat accgccatga tttactaatt tattcccgcg catttccggt ccatcttctc
    15901 caaaatttgc tgtaaaaatc acgattcctg aagcatctgt ttttacttgg atcgatactt
    15961 tttcatctgg gcttgtcaat tccgcagcat ttagtaaacc agattgtttt ttcaacaaaa
    16021 atggatgatc gatgccgccc actatcattg attggcgaaa atcactggca aagacctcac
    16081 ctaatttttt aggacgttgg aagtcaaatg ctgtgccact tactgaaact acttctcctg
    16141 taggcgttgt atctttattg agcgtagcaa ataatcggct attcaaccaa agtatatgtt
    16201 cgtcgattga ctgtgctgga tcaccggtta aattgaaata gacatgattt gtaggattga
    16261 atactgtttt gcggtcactg atcccttgga ccgtcactcg ccatgtatca tcgttcgtca
    16321 gcgtataccg tacttctaca tctaaagttc caggaaatcc gtttcccata tctgggctag
    16381 tctgatagaa aatgaccgat gcttcgtttt caccattgat gactgtataa tgccactgct
    16441 tggcttcaaa gctaggtgca ccaccatgta aacaatgacc agtgtccggg tctgttttta
    16501 cttcgaacgt ttcatcatct aatttgaatg tgccattcgt aatccgtcca gccatacgtc
    16561 cgattgaagc accgatataa gggtcttttt ctagatattc ttttgcttca tcaaaaccta
    16621 agatcaactc tcgtttttca ttatcgatta ttgtttgaaa atgaacaacc cgtgccccaa
    16681 gatcactgac agacaaagat gtcccatttt gattcgtgat cgtaatcaag tgatagtttt
    16741 cgccaaaatt ttgttcattg atttccatgt tccttgcacc ctcttttctt tcatttgcac
    16801 tgattccagt atacatggaa accgttttct taacagctta ttccgtgagt aacgagaaaa
    16861 aatttactat attctaataa aagaccgtga aaaaaaggtt gtgacaaaaa tattgtcaca
    16921 acctcttttt ccgaacaaac ggtgttcaaa agcttgcatc tgcggtaata agcccaacca
    16981 aacgaaaaat atgaggagct atttttgctt ggctgttctt attacttgat gcaggacagc
    17041 tttttcacaa tctctttttc aatacgattc cagtatataa ccatgtcgtc agtacatagt
    17101 agatgaaata caatacgaag aagatcgtaa acggaaccca aatatctttg tatggatttt
    17161 gcatgaatag accaaacatt tgtaatccga ataagacatg gactacacct aagattcctg
    17221 gtgctaagaa taacacgcct acttctctac ggatcgattg tttaagcaac ccgcgtcgtg
    17281 ttcctatctt ttcaagcatt tcataacgag caatgtcact cttgcttcct gacaagatct
    17341 tgaacatcaa acagctagca agcatggtca agaaagctaa acctaggaag aatcccataa
    17401 attcaaaacc agagaataga ccattataca cttcatacat ttcatatttc tggttgctgt
    17461 actctacttt ttgcaaagat ggattgttca ttttattttg agcaacaagt gtttcgattg
    17521 gtttcaggtt tgcttggaag tcttttacct gaactagctg taaagtggat tgtggcaagt
    17581 tcagttgatc aaattctgat tgactgacta ccttgatttc tttttcttgc tgttccggca
    17641 attcatagct tcgaagttcc tcttgtacag tcagttcatt cattttttta gcaggaactt
    17701 tttctttcgt gatcttcggc ggtgttccat cttcatgttt tatcatgatc aatggttgag
    17761 ccgcaaattc acttgcatta tagtaaatcg ttcccgcatc ttctttttga gtataagaag
    17821 cagaaagtgt tggtgaaagt tctttgattt tttgctgatc ttctgcccga ggattattca
    17881 tcactagatc atatccagaa ttgttttctg tcaagtctgc aatttcattt ttaaaaccta
    17941 atcctaccgt caaagcgccc agtgccaagg caaacagcat agcgaccatt gagagcattt
    18001 gtgtatattc acgaatacga aagtttaact gtgacaatgt gaaattgttc aattttttca
    18061 atgaaatctg ttctgatttc tttagtaatg ataaaaagaa aatgatcaca gaatggaaaa
    18121 tgaaataagt tcctaatatg atcgtaacta aggctacccc tataccaaag acttgatagg
    18181 tccccacgtg tttcatcata tagtaaccgc agcctagaaa agcaattccc agtactactt
    18241 caaggaaaaa caagaattta tgctgcttga ttcttgttgg tgtctgatcc gcacgaatca
    18301 atgctaaaat cggttttttg acaatcgaaa aagcattgac aacagcagct aataaaaaca
    18361 aaacagcaaa aaacaaaaca gtgatcaaca acccgtgaat actaaaaggt gtaaaatgcg
    18421 aaatattgag atctaactga tccactaata atcggtggac gatacttgtc aatcctacac
    18481 cgacagctga acccactaaa gtagctgcga tccctactaa gaatgtttca gaaaaaatca
    18541 tctgtgcgat ttttctgcct tttgctccca gcatcataaa cattgcatag tctttttgcc
    18601 gcatcgtcat caaaaaggaa tttgcatata aaatataaac gaatgtgatg atccctagta
    18661 aaacagaacc gaatcggaaa atgataactg tagccgcaac gatcgtatta cttttcaaaa
    18721 aagcttcgtt gcttgccatt gattcaaaca tgtaaaaaat agcagatgcc ataactaatc
    18781 ctgagaataa caccaagtag tctcttaaac ggctcttgat cccagtaagt gataatttcc
    18841 aaagcatagt caatctcccc ctactgttcc aagtttccaa gaatagatag tatttctcga
    18901 tagaaggaat cacggctttg atctccacgt ttcacttctt gatggatacc gccatccttg
    18961 atgaataaaa tacgttggca ataacttgca gaaaacggat catgcgtcac aagaagaatc
    19021 gatacatgat cttttgtatt caattcgtcc atcgtatcta aaagatcccg ggcacttttg
    19081 gaatccagcg cacctgtagg ctcatcccct aatagaatcg ttggttgtgt gatcaatgca
    19141 cgtgcggcgg cgacccgttg tttttgtcca ccggaaatct cagaaggata gctttccaaa
    19201 atatgagaga tggataaacg ttctgcaatt ttttggacac gttgcttgat ttcttttggt
    19261 ctgacgcctt gtaaagaaag aggaacagcg atattttctt gtgctgtcaa gctttctaat
    19321 aaattgaaat cttggaaaat aaagccaatc tcattcgcac gaaaatctgc tagttgattt
    19381 ccttttaatg tggtcacatc tttttgattg attcggatat gcccatctgt tggtttatct
    19441 aatgtggata aaatattcaa aagtgtcgac ttaccagaac cagaagcgcc catgatccca
    19501 ataaattcgc ctttttctac atcgaagctg atgccgctta atgcttgcgt ctttttctca
    19561 ttgttttttc cgtatacttt tgttacattc tgtacttcga ctattttgtt catttctttc
    19621 cctacccttt aattagtttt tccagtgctg ttttcggcac gtcttttttt tcgacttctt
    19681 catacgaagt gacaccttta tttttgttcc aggtgatttt taaatatgct cctttacgaa
    19741 gcggtcgagt caattgtccc ttgaagtcca gttctttttc cttaccctct ttcgtatagc
    19801 caggcaggat atatctatag ttgactgttt cttgtccaga gtctgatttt gaaactgttt
    19861 ttgtaccatc tgtcgtaatt tgtacatagt attcttctcc acccataact acgtaatctg
    19921 cgattttcac ccctgcaagt accacaacga tgaaaacaag tagaccgatc agaagttttt
    19981 tcactttttc cccttctctc ttactttata tactgattat agaaaaaagc gaacaattct
    20041 gccttcgaat caactatcat tcttctttca attttgtcat tttctttaaa acaactaaaa
    20101 aaagagttca agacaataat ccgtcctaaa ctccttcttc ctatcttaca aaaagtatgt
    20161 cacttacggt ctttcatgtc gcctgtattg ataacaggtt gcgttccttt gtctgtaatg
    20221 attgagacaa gtaaattatt gatcaattgg actttccgat catcggtgaa attgatttct
    20281 tgtccatctt cgatttgctc taaggccatt tgagtgattg tgacagctcc ttcaacaatg
    20341 gtttgtcgag cagacaaaat ggcttttgct tgttgacgtt ggagcatggc actagcgatt
    20401 tctgttgcat aggctaaatg gttcaagcgt gtttcgatta cttcgacacc tgctactgct
    20461 aaacgctctt gcagctcttt ttgcaactcg tcagacactg ccgtcgtgtt tcctcgaagc
    20521 gtcaaatcat tctcattgaa tgtatcatac gggtattggc tagcgatatg ccgaatggct
    20581 gtctcacttt ggatttctac aaaatcttga taatacgcta catcgaataa tgcctttgct
    20641 gtatcaacga ctttaaagac tactactgct gagatttcga tggggtttcc atccaaatca
    20701 ttgactttta aaacagaact attgaaattc cgaactttca acgaaacagt cattttttgt
    20761 gccaaaggta tcgtaaggaa aaagccattt tctcgaatcg tacctaaata ttgtccaaaa
    20821 aacaaaatta cttttgcttg attaggactg actacagttg ccgaacttaa caacaataac
    20881 gtgatcaacc aaagaaaaat acttaaaaaa agtgcccaaa ctttcgcttg ccacatacct
    20941 aaatagaaga aaaacaatcc aactaaagct aatacgacca atccgacaat ccctgcataa
    21001 ccattgatat aaaatgctct tttttctctc atgctacttc ctgcgctcct ttatccttca
    21061 ataaatatct aatgttactt ttttaatttt ttttcgaata cgcgccatta caccttctag
    21121 ttctaactct ttgtcttcag tgatgtacca accccttgcc tgtgtccctc cggctgcctg
    21181 cggtaaataa tccaattttg ttcgaaccca gtgcttttct tctgtaaaat agtcttctac
    21241 caagacctct agatccatcc ctgctgaaag aggcaaatct tcccgaacag atttaaagat
    21301 aaatcgttcc ttcttcccat catacttcaa tattcctgta tcgataatcg gtaaagaata
    21361 ataatccagc tctttcttca ccgtcaataa atgtttgaaa acttcatggt acacttgctc
    21421 taattggcga ccttctgaat tgttcgggta attaaccatc actttttctt tgtctggatc
    21481 atagaaggtt tggcttagtt gagtgatata agtatgtaat ctttccattt ctgtttttaa
    21541 cttttctctt tgaccttctt ctaccttcat atactctttc accctgtttt ctttttagat
    21601 attttctttc ttattttact atagttatgt gcgttaagcg atgaaaaaca gcaaagattg
    21661 gaaggatctt tgctgttttt tcttaaaata tccagttgag tacaggatca atctgtttca
    21721 ttgtcctctt gatccgtcgt aatattgatc gatgtgtctt tagcagtgat ctttagcttt
    21781 ttctgtcctt gttctaacaa agtatttgtt attggctggt agtctttttc aatataagat
    21841 tcaccagacg tttggatagt atagccactg ttttccatct catgcaaaga taaattgcca
    21901 ccgtttagga tgacttttcc ttctttgctg atcaaactgt cttctagatg gaagttgctg
    21961 ttttcacctg tgacgatcaa gttatccaca ttggcctcat ctaatcgaag tgagcctaat
    22021 tcatctttcg cagttattgt tcctcctgta aattcattga acgataccca gccatcatgt
    22081 gtcttagtat tcactttttt gattgatact tcgttgatat caattgcact attattccct
    22141 tcgattgtca attcagataa ttcatgatct gcagggacaa caatagtgat ttgtgcactt
    22201 gtgtcttcta aactaatgat tgcacctgtt gattttttga ctgcattcac agtcaacacg
    22261 ccatttttct cctttatttc tggcttttct gtcttatcat aagtcgtttt tatctcaaaa
    22321 tccaatccgc gtatgaccga aactgtttga ttttctgcat ggatgacgat tttgtttgca
    22381 tccttgattt tttttgtctc agtaaccata tcgatcaacc gaggaccatt atcccaagtc
    22441 agactctttt gagcgcctaa tgtataagtt gcacctgcta ggaggccacc agtaagcatc
    22501 agaccaatac cggtaatcaa aaaatgtttc aatctcaatt tatgtgtcct ccttcaaacg
    22561 cccgctgaat gcagatctct ttttgtaata aaacgtcgtc ccatccattt aacaaagttc
    22621 atcatgacat aaaaaagccc ttttgttcct ttcagcaaca gtggcgaaat aaatactgcg
    22681 cttcctgtgg cgaacagacc gacaccgata aagaataatc cactcagaat cgattgtcca
    22741 aacacactga ttcctgagat gatcatgaat aggccaacac ctaagatact gatacataaa
    22801 acatagatac taaaaatgag cgctagtaaa gcaaccaata gtcccccaat aaccaatata
    22861 aaccccagtg ctagtggaat gaggacagga gaagcaaata aaccgataat gattaaccaa
    22921 atcaggcgaa tttgtctttt tggatttttc actaactcgt tttctgcttg ttcttcttct
    22981 gaaaagtaac tgatttttaa ttttaaggca aactgctttg gtgtaccata ttcatcaatg
    23041 atctgatcta atgtttttcc agaatctaaa aaatattcct cataatactg gatcaaatca
    23101 aaacgatcgt cttcaggaat ttcttgaaga tgatccacaa cttcattcaa atattcattc
    23161 atttgtgctc tcctccaata accggtctaa agaatctcga aaaacaatcc attcatcaat
    23221 aatttcttct aaatgacgac ttcccattgg tgtcaacgag taatatcgac ggtttctgcc
    23281 ttcaaaaggt tcatcatagg tctcaagata tccttctttt ttcaatcttc tcaaaaccgg
    23341 atatagtgta gattcactga cagagagact ttcttgtacg atttttgtta aggcataccc
    23401 atacatatct tccttgctta atgctgctag cacagtacca tcaagaagaa cagttggaat
    23461 ctggatgtgc attacgtcac cttctattct caagtgtaat attttatttc aatctatatt
    23521 atacaacata taatatagtt tcaaaatcag tttgtcaaat acaaaaaaac tgactaaaag
    23581 atttgatcct ttttagtcag caaaaaatgg cttctctttg atttttatta taacagtggc
    23641 ataatccaat ttgtccacat ccagctcagt ggcatgataa cgatcattgc aggtatcatg
    23701 tcagctactg ggaacatttt cacgttgatc attctaaaac cggtagcgac catcaagaat
    23761 ccgccgcaag ctttaaaatc taagatcatg tctggtgtag tcaatggata aataaatttt
    23821 gccaataaga acaaaatata aaaaataatg aattggggaa ttgctatcac agaaacgaca
    23881 taccctaaat tacacgcaaa aatagctgct gtgaaaaaat caaggacaga tttagaaatc
    23941 aaaatcgtac tgtctcctgt catcccagaa tctaaacttc catagatccc cgtaccactt
    24001 gcacaaaata gaacaatgac cgtcactaat gtgctgataa attcatcgtg ggtcatacca
    24061 agcttttcac tagggaagat tttagaaata ggtacttgca tcagcgcagc ccccttattt
    24121 atcccattac ctaaatgaac aatcagacca atcgctgttc ctatcactaa agcaaaaata
    24181 acagccggca tatttttcat cggcgcaatg gaatagattc ccatccccat cgaacacaca
    24241 ccaaaaacca tcgtcagctg tgctttaaaa tgttcactta atttatttcc tactaatccg
    24301 ccaattagtc cgccaagcaa aacggaacct acattgatca aaactccagt tggcatcaaa
    24361 aaactcctct ctttttctac gaagcgaaaa aaatcagaaa aatttctgta attattgctt
    24421 tcgctgcaag aaagattgta acagacagag aagattacac caattcattt tttgtgttaa
    24481 agtattcttt attggaataa gtaggaggaa tatgatggat atccataaat tcgaagtttt
    24541 tcttgattta gccgaaacaa tgaatttcac caaaactgct gatcgacaat tcaccaccca
    24601 aggaaatatt tctaaacaga ttctttcgtt agaaacagaa ttagacgtca aattattcga
    24661 acgcgcccat agaaaaatcg aattgaccga agctggaagc ttattgctcc catatgtaaa
    24721 aaatgtcgta gaacaatacc atgctatgca agaagtgctt gaaacctata cgaaagacaa
    24781 caatctttca ttaaatatct taacgatacc gacaatgatc aattataaag gtttttccaa
    24841 aatcaccgag tttttgaaac gccatcctga atttacagtc cagttgaaag aagtggaaag
    24901 tgtcgaatta acttacaatg acgccaatga acatagtgac acgatccact ttgctagaaa
    24961 tttccaggcc ccaccaagtt cgattgagtg gttgcctact gaaacagacg attttgtagc
    25021 agttctacct aaaaatcatc ctttagctag taaaaagaaa ttggatctaa gcgaactgaa
    25081 aaaggagaac tttttgctcc ttggccccac tacgaacttg tatcaacctg tactagatct
    25141 gtgtcgcgaa gctggatttg aaccaaaaat ctcttataaa ggagcaagaa tcgacttgat
    25201 catcaatatg attgcaaatg acatggggat tgctattgtt atggaaaaaa cagtcaagaa
    25261 tctattaaaa gaaaatactg tgattctccc tatttctcca acaacagaga gttatcttgc
    25321 atttactcgt aaagtcggtg agcattcttt ggcttcagat acattttggg agtatttaaa
    25381 tcaagaagaa aactaagccc tacattttct gtccatctat ccacccaaaa aaagagagcg
    25441 acgcatgact ttcgctctct ttcttgcatc atgtccttgt ttcaatgaca cctctaacat
    25501 ttctcgtagt tcaataagaa agcagcgatt tttatgccaa aagaaccgta agaacatttc
    25561 cctaatcaca aaaaaacgac taaaaacccc taaaaacctt caatagaatt tttaggggtt
    25621 ttattctttt tcctattgtt tacttaaccg tatcattgct gtcgttccta ctccttcgat
    25681 actgctataa tcaatacgtc catcatgcag ttctagaatt tttttaacca gtgaaagtcc
    25741 tagcccattc ccagaaattt gacgacttgt atctccttga taaaattttt caaacattcg
    25801 atctctggtc tcttcattca ttcctacacc tgaatcggtc acttccagtc caatctctgt
    25861 ggctgtctca aatagtttga tatgaatctg tccattgatt tgattgtact ttatggcgtt
    25921 atccatgata ttcagccaca cttggtacaa aaactcttca ttgcctgtgt aatttactgc
    25981 agcaagttcg atatccaatt ctagctgttc tttctcccat ttaggctgca aaaaaacgat
    26041 tacttctctg atttgttcat ctaatcgata ctctttcttt tccagttgga tacgctggtt
    26101 ttccagtttg ttcaatttta agatgttctc tgttaattgt gaaagctgcg tgatactttc
    26161 gataatccga tataaaaata tttggcgttc ttcgtcagag agatttgggg cttgtaacaa
    26221 ttgaacatat ccttggattg ttgctagtgg tgttttgaat tcatgggata cactggacac
    26281 aaagtcattc ctcagtgtct cgatactatt gagttcttgg accataacgt tgaaatcttt
    26341 atacagttgc tgtacttctg ctactttctg ctgttcatcc attcgaatcg aaaaatctcc
    26401 cgttgctacc agactcatat ttgtccctag cgcactgatt ggatgcagga tacgccgccc
    26461 aacaaaaatc gatattcctg tacccacgac catactgaac cccgcaaaga cgaataacgg
    26521 gtataacgtt ggttttccag ccgattcagc aggcgagata gaatgtcgag acaataagaa
    26581 aataaaaccg agaaaataca gcagtgtcac taagatcgaa atgaagacaa tactagcaaa
    26641 atagatccat aattgtgaaa atagcttttt tttcatcttt aaataaccgc cttatagcct
    26701 agtccccgta ctgtgatgat ttgaaaatct tccgtctttt caaaacgggt ccttaaacgt
    26761 ttgatatgta catcgatcgt tcgttcttct gtattcgtat ctaatcccca aatatcatcc
    26821 atcaactgtt ggcgagtgaa gatcttgttg ggataagaca gtaatttata cagaagcaga
    26881 aattcttttt gaggcagaat caacgaatgc tgatcttgta acactgtata agcatctagc
    26941 tctaattttg tttgtccgat tatcaagcta ctgctgtaat tgatctttga acgtcgaagc
    27001 aatgcttcca cacgtaggat catctcattg acatcgatgg gttttgtcat ataatcatct
    27061 gctcctaatt tgaacccgcg tttcttatcc tcaaaatcgg cctttgccgt aatcattaga
    27121 acaggtgtgt caaatctggt gctacgtaat gagtctagca agtcatagcc atccattttc
    27181 ggcatcatga tatctgtaat gattaattct atacgtgtcg cttctagttt tttcagcgca
    27241 tcctctccat tcaatgcggt gatgactgaa aagcccgctc gttccaacac agaacggtaa
    27301 agcaactgta attgcggatc atcttctact actaaaatac gattcattga tctccctctg
    27361 ttttttgaaa aatttctaga ttttttcttg tagttcatct tcagttaaca tttatttcgt
    27421 atactacgat cattgttctg atatacttat tagtataccg aatatttcaa tacgggagaa
    27481 gggtgattat gcttcaatta aaagatataa aaaaatatta taaagtcggc gaaaccacca
    27541 ccaaagcatt ggatggtgtg tctgtcgctt tcagacaaaa agaattcgta gcgattttag
    27601 gtcctagtgg ttctggtaaa acgactatgc tgaatgtgat tggtggacta gataattatg
    27661 attccggtga tatggtaatc aacggaaagt ctactaaaga ctttaaggac agtgactggg
    27721 atgcctaccg aaacaactct atcggtttca tttttcaaag ttacaacttg attggtcatc
    27781 taggcattat tgaaaacgtt gaacttggga tgacattaag tggtgtttct aaagacgaaa
    27841 aacgaaaacg ggcagaagat gcattgcacc gagtcggctt gacagatcat atgcataaaa
    27901 agccaaatca gttatctggt ggacaaatgc agcgagtggc catcgctcga gcactagcca
    27961 atgacccaga tatccttttg tgcgatgaac caaccggagc gttagataca gaaacaagta
    28021 tccagatcat ggaattgatc caagagttat cgaaagaaaa attagtgatc atggtcacac
    28081 ataatcctga actggcccat caatacgcag atcggatcat tgaattttct gatggtaaga
    28141 tcttaacaga ttcccatcct cacatcgaac gaccaaaaga tgatcaattt aacttacgca
    28201 gaacaaagat gagtttttgg acagcgttaa agctttcctt caacaatatc cgaacaaaaa
    28261 aaggacgtac tttcttgacc tcctttgctt ccagtattgg gatcatcgga atcgcgatcg
    28321 ttctgtccct ttcttctgga ttccaaaaac aaatcgataa cacgcaagct gaaacgatgg
    28381 cgaaatttcc gattactatt tctaaggtaa cgactaacca gactcgtgac gatgccggat
    28441 tgggtgcaag caaagcggat tatccagaca gcaaaacgat tactgcaaaa gtcagcgaag
    28501 aagataaagc acagcacacg aataagattg atcaaacata cgtcgattac gtaacagaca
    28561 tcgatcctaa cttaagtaat aatatcggat ttacccgtac tacaggaatc aatcttttac
    28621 gtgatgtaaa tggcaaagta cagcctgtca gcttctcgaa tcaaaatcct gatacggaat
    28681 ctctttcttt atctagtacg atgtcagcaa tgacaggtgt gggagtgtca agtttcccta
    28741 cacagcttga cactagcaaa gaaaactttt taaaagacaa ttattcttta cttgccggaa
    28801 gttatccagc ctcagcaaca gatgtcgtgc tgatcgtaga cggcaataat aatactaata
    28861 tcaacgcatt gaagaatctt ggatttgatg tgaaagaaga cgaaaaactt gattttgatg
    28921 atatcgttgg tacaacattc aaattagtaa acaacaatac ttactataca aaacttccaa
    28981 ctgggaattt tatccctaat acagattatg atgcgatgta tcagaatgct tctgatgaat
    29041 tgaagatctc ggggatccta cgcgtgaaat cttcttctac aatgaatctc ttatctccag
    29101 gaatcgctta tagcgatcaa ttaacgactc aaatcgtcaa cgaaaacaaa gaatcagaaa
    29161 tcgtcaaagc tcaaagagac agtggtgtaa atgtcttaac tactgaaaaa gttgacgaaa
    29221 atgccaaaca aacattgctt agttatttag gtggagacag cttgccttca agtatcatga
    29281 tttatccgaa caattttgaa gacaaagaaa agatcttgga ttatctagat gattacaata
    29341 aaggcaaatc agatgaagat aaaatcattt atactgattt agctggaacg atgactgaac
    29401 tgactggcgg attgatggat gctatcactt atgtccttat tgcttttgca gggatctctt
    29461 tagtaacaag tatgatcatg atcagtatca ttacttatac atctgtgatt gaacggacaa
    29521 aagaaatcgg tgtactaaaa gctttaggtg cacgtaaaaa agacatcact cgcgtatttg
    29581 acgcagaaac gtgtatttta ggtatttctt ctgggatatt aggtgtgttt attgcatggc
    29641 tagctacgtt ccctatcaat agcatcttat acaacatgac ggatctaaaa aatgttgctc
    29701 aactaaatcc tgtccatgcg attattctag tgatcgtctc tactatattg acgatgcttg
    29761 gcggtcatct tcctgcacga atggcagcga aaaaagacgc cgctatcgct ttgcgtgcag
    29821 aataaggttg tgacaaccac atttagattt gagtcataag agcgctgaaa tagtagaaaa
    29881 cagcaagaaa cgaggaagta atatgttcct tgtttcgcgc tgtttttttt aattattttt
    29941 tataattctg gtatactttt ctttcctttg taccacaact cttctggcgt atacctgtct
    30001 tttccattta tgaatactgt tccatctgct tcaatcgtta cttcagtccc aatccacata
    30061 ttacgagaaa gtgcgagaag attttttctt tttcctcttc ctttttttcc aaattgcaag
    30121 gcatcggtta tctcttctgt ccaatgagat ttcacgtatt ttacttctcc gttttcaaat
    30181 gtaactttca aaatattcgc tatattttcc acttttactg ctttaggaaa tgactccttc
    30241 atttttttca ctttccttcc tccaacacta agtatgcgct ttcttttagt gataagtata
    30301 tcatagttta ttctattatc aaaaaaacgc atccattcca ttgtagaata ctttaccgaa
    30361 caagttgaat tgttttcctc tttttcacct tttactatag ataggtaaag acaactaagg
    30421 aggaatctct catgacagat caaccgtatg atttaagaaa agtcatcgaa gaactaaaaa
    30481 cacttccagg tcaatatcac gaaacagata ttgaaatcga tcctgaagca gaattatctg
    30541 gtgtttaccg ttatatcggt gctggcggta cagtaaaacg tccgactcaa gaaggaccag
    30601 ctatgacttt caacaatatc aaaggtttcc ccggcacacg tgtgaacatc ggaacaatgg
    30661 ctagtcgtaa acgtgtaggt gaaattttgc accatgatta tcgaacactt ggtcgtttct
    30721 tgaaagacgc tgtagaaaat ccaatcaaac ctgtgaaagt agacaaagca gacgctccag
    30781 cacaagaagt cgttcatcta gctaccgatg cagacttcga tattcgcaac attttgcctg
    30841 cacctacaaa tactcctgaa gatgctggtc catacatcac aatgggtgta gtcttaggat
    30901 cagatccaga aaaaacaatg agcgatgtaa cgatccatcg tatggttcta gaagataaag
    30961 atacgatcgg tatgtacatc atgcctggcg gacgccacat tggtcacttc caaaaacaat
    31021 tcgaagcctt gaataaacca atgcctatca ctatcaatat tggtttagat ccagcaattg
    31081 ccattggcac gactttcgaa ccacctacca ctccacttgg ctacaatgaa ctatggtgtg
    31141 caggagcttt gcgtaacgaa cctattcaaa tcgttgatgg tatagcagtc gatgaagtgg
    31201 gtattgctcg tgctgaattc atcattgaag cagaaatcat gccaaatgaa acaatgcaag
    31261 aagatatcaa cacgaactca ggaaaagcaa tgccagaatt tcctggctat actggggatg
    31321 ctaatccagc tgtacaagta gtgaaagtca aagcagttac tcaccgaaaa gacaatccaa
    31381 tcatgcaaac aacgattggt cccagtgaag aacacgtatc tatggccggt attcctacag
    31441 aagcaagtat tcttgccttg gtcgataaag ctattccagg aaaagtagta aatgtctaca
    31501 atcctcctgc aggtggtggt aaattgatga ccatcatgca aatccgtaaa gaaaacgaag
    31561 cggatgaagg tattcagcgt caagcagcta ttcttgcttt atcagccttc aaagaattga
    31621 agacagtcat tttagtcgat gaagatgttg atattttcga catgaacgat gtagtttgga
    31681 ctttcaacac tcgtttccaa ggagataaag atatcatcgt gatcccagga atgaggaacc
    31741 atccattgga cccatcagaa ttaccagaat ataatcctgg ctctattcgt ttccgtggga
    31801 tgagtgcaaa aactatcttt gacggtacag ttcctttcga tatgaaagaa cattttgtac
    31861 gtgcaaaatt ccaagaagta gaagattggg aaaaatattt gaaataatga atgatctgca
    31921 actgttttga catcataaaa aggagtgcgg aaaatggaac aattggcaca acggaagaaa
    31981 aaaatcgtga ttggtgtaag tggcgcttca ggcaccattt atgccatcaa cctagtgaag
    32041 aaactaaaag aatatccgat gatagaaaca cacggaatca tcagtccatg ggcaaaacag
    32101 aacttaaagc tggaatccga cttatcacta actgaattcg aaagcctgtt tgattaccat
    32161 tattcaaaca aagatttagg tgctgctgtc gccagcggat catttttgac cgatggaatg
    32221 atcatcgtac cggcaagtat gaaaacagtc gcaggtatct ctgttggttt aggcgacaac
    32281 ctgattgcac gtgctgctga tgtaacaatg aaggagcaac gaaagttagt cattgttcct
    32341 cgtgaaacgc cattaaatgc cattcacttg gaaaacttga ctaaattagc acgactaggt
    32401 gtgcaaatca taccaccaat tcctgctttt tataatcagc ctcaaacgat ccaagatctt
    32461 gttaatcacc atacaatgaa acttttggat caatttggaa ttgtaaatga gcattcaaaa
    32521 cgttggcagg gtgattaata tggaaaaaga aacgatagtt gaagtaacca aactaggttt
    32581 cacgatccaa gctgctgtta aagtaatcgg taaagactta ttgatcactt taacaggcgg
    32641 agatacacca catatcggaa cagtcactac attttctcgt gatacagaga tgcaaactgt
    32701 acgtttccct agccacgatg gtcggttcca taaagacgat gtcttagccg aaaaaatagc
    32761 tgtgatcatc aaaactgccg ttccgggaag ttgtacgatc acttccggta tccatgttga
    32821 ccatatctcc aatgcacaaa tcaacgcctc ttttccgatg tcagaagagt taggttacaa
    32881 gattttagat tggttggaaa aaatagattt ccatatcaaa gaaccagttt attataaaga
    32941 tggtgaaaaa acattatgaa agaagaggtt gtgaaatcag cgttatgacc tgagtatgag
    33001 agaaaaaaat ggaaaaatag cttctcatat tttgccgttt ttgagctaat accgagggac
    33061 agctgattga acgccgttta tcaagaggtt gtgataaagc cgcttcgttt cgagtagtaa
    33121 gaaaaatttt gtaaacatgg gtttccacat ttttgcataa atttgactta ctaccgagaa
    33181 actggctttt gaacaccgtt tatcaagagg ttgtgacacg tcttttgtca caacctcttt
    33241 tatttttgct tcactgtaac tgctacaata tcaccgattt cttttccgat gtttttacga
    33301 atatcttttc gtagtccaag gatgtagcag actgaaccat ctggattttt cacgcccata
    33361 ttcacgatac tgccttcata cggcatattg tcaaaagttg cttggacttt aactcttcca
    33421 gcaccgaatt cttttcttat atcatacgga aaaataatat atgccccgcc cttgtcgact
    33481 tcggaagcat agatttttga ttcaaaagaa taaatttccc tcattttatt tctcctattt
    33541 tttacatcta gtatagcata gcaaaaaaaa gaaggaactg tgacaaaagt cttgtcgcag
    33601 ttcctttttt cgaataaacg gtgttctcct gccaactcct cggtcacaag tcacaatagt
    33661 caacaaacat gagaagctgt tttttgaaat tgctccttgt gactcagagt taaacggcag
    33721 gctcacaacc tcttccttaa acggtgttca aaagcttgca tctacggcac taagcccaat
    33781 cagatgaaaa atatggagtg ctatttttat ctgattgttc ttactgcttg atgcagagca
    33841 gctttttcac aacctcctac atggtgttca aaatattatt cgccttttgc ttctttcact
    33901 aattttgcga atgcttgttg ttggaattca gcagctaagg ctttcgtacg ttgttcttgc
    33961 tctgctttgc tcatttttgg attttcagtc attgctttta ccataccgtc ttgaacataa
    34021 gcttttgctt cactttccat cgtactttgc tgtttcttca ctaattcagc taacttggct
    34081 gcttggtccg tacttaattc tacccatgtc tctgggattt tgaatgtatt cactcggctg
    34141 tcatattcgt gattgttatc agcaatattg aaccagaatt tatacatgtc gtttttgtat
    34201 tcccaataag tagttttcgt cactttttca gcatcgccgt ctgtcttttc tactttattt
    34261 gtttcgttat ccgtaccagt tgcttttggt ttttcttgtt tttcatttgt tcggtaaaga
    34321 tacacttttt cttttccatt ccctaaagct ttatacaaaa gcatatctgc tccttgggaa
    34381 tcagcactgg aaacaatttt ttctgttttt gtttccgtta ctttttccat tccaaagtga
    34441 ttggacaaat tagcgacgat caaacttagt gaagcgacga agattagtcc gaaaatcaaa
    34501 gataaaaagg tttgccatgt ttttttagcg aacacgttcg ttaaagcaaa agcaagcaag
    34561 ctgacaatca aaactccaat aatcatgcat tatcccccgc tttcattaga acaggttctt
    34621 ttttcttcaa taaaaaggca ataataaagc caagaatccc aaagatgatc gctacgataa
    34681 acgcggcatg gtaacctgac agtgtggcat tgattgcctg atctttataa gctaatggcg
    34741 tattatccaa tacagatttg cttgggatat ttgcttttgt cacattcgtc aacacactga
    34801 tcaaaatagc tgtaccaatc gagcttgcga tttgacggaa tgtattattt accgctgtcc
    34861 catgactgat caagttattt ggcaatgcat tcataccaga agtcgttacc ggcatcatga
    34921 ccatggagat cccaaacata cggatcgcat aaaggatcac gatatataaa actggagtcg
    34981 tttttgtcaa gaaagcaaat ggtgcagttg ccgctgtcaa aatgaacatc cctgtgatgg
    35041 ctaaacgttt cgcgccgtat ttatcgaaaa tcgcacccgt cacaggcatc atagccccca
    35101 tgatcaatgc acccggcaac aacatcaagc cagaatggaa tgctgattct ccacgaatat
    35161 tttgaatata caatggtacg accatttctg cccctaccat cgccatattt gttacaccag
    35221 ctaaaacagc tgcaatcgtg aatgtagatg atttgaatac acgaagttcc aagaatggat
    35281 gttccaaatg taattgacgc catgagaaca aggcgatgaa aatcacacca acaactaaga
    35341 aaccaattac tttcaagctt ccccagccgt catctcctac actagaaaat ccgtaaagta
    35401 aactaccgaa tccaatcgtt gataaaatag cggataaaat atcaatgcta ggatttgaca
    35461 atggaataac acttttcatt aggaagaatg ctaaaaccaa gacaagaaca acgattggca
    35521 gtaccatacc aaacaagtca cgccaatggt agttatcaat gatataacca gataatgtag
    35581 ggcctaaagc aggagcaagt ccaataacga tccctgacat ccccattgct gatcctcttt
    35641 tttctggcgg gaaaatagat agcatgatat tttgcaataa tggcatcgat actcccacgc
    35701 cgcaagcttg gatcaagcga ccgattagta atgtactaaa attaggtgct gtaaagcaag
    35761 taatcgttcc gatcaaaaag atcgtcatgg cagaaagata taaatttctt gaactgaatt
    35821 tattgatcag ccatgctgtt attgggatca tgatcccgtt gactagcaag aaaccagtcg
    35881 ttaaccattg cacatcagac gctgaaatat cgaaagcttt catcaatgta ggaaaagctg
    35941 tcgtcaataa ggtctgattc aaaacggtac aaaatgtacc aattaataag acaacaacta
    36001 aaagtgtccg attatatggt tttccgtaaa tatctacggg ttggttgtta ctcatatttc
    36061 tccttctttc tgaatttttt ctgaaactac tagaatactt tagtcctaaa ttcgctattt
    36121 gtcaacctac ttgacacata agttaatacg gatataatta agggaagcaa gaggacaaaa
    36181 ggagtgacaa aaatggttgg ggcttccctt aaacgtttgt tggaaaatga tcaattggtt
    36241 gttggcatca gtgaattaag tgatatcgta ggtgtgtctc cacgtcaatt aaggtattgg
    36301 gaacaaaagg gattcattcg ttcgatcgaa gctgatgcga attgtcctag aaaatatcgt
    36361 ttacctacag tcataaaagt agaactgatc aaaaaatatt tagatgacgg atttactttg
    36421 tcaaaagctg cagaaaaggc agaaatccgc caaaagaaaa tgcaccatgt ccgtaaagtt
    36481 ttttcaaaag caatcaaaga tatcgaattg atcgacgaac gctatacgat tttacaaatc
    36541 ggtaacttca aagaagagaa tgctaagatg tacatcattc atgacaacga gacagacgaa
    36601 ttaacttaca agctccttcc tgtcgatgaa aaagctgact acgaacagtt gttaaaagaa
    36661 ctctaaacgc cactaatctc ctaagtacca agctttttaa taagtagatg ctacataaat
    36721 acttgaaaaa aggcctgttt gaacataaga atatccgaac ggtgatctgc gtttctttca
    36781 tatagaaagg aattctccat cactgttcgg atattttttt atttttttcg gttactttct
    36841 tctaatacat ctgttagcca atttgcatat tctgtttccc gattgatcgc atgcgctaaa
    36901 atcaggtagt gtccgtaatt ttcatgtttt tctttttcag taggaaagat cgtttttttt
    36961 cgctctaata gatgaagtcg tttttcttca tgaaggtcac gctgctgctg aataatttct
    37021 actaatgccg gatcattcac gtctttgacg aaatacaatt tcagaacaaa ttcgtctcga
    37081 ttaaccggta gttcattggt tggtgtatga atccactctg ccaattgttc cttcccttct
    37141 tctgttaatt cataaacttt cttttccaat ttttcacctg tgatctctac ttgatgctga
    37201 atgattcctt gctcttccat ttttgcaagt tcaggataaa tctgactatg ttttgcttgc
    37261 caaaattctc ctatttcatt tttgaacgat tgattcaatt catatcctgt catcttcttc
    37321 ttacttaata accctaacaa aatatatcga agtgtatttt ttcttgccat cagttttccc
    37381 tccttgacaa atctttaaat ccattctatc ataaatgagt aatcgaaaac atgtagttta
    37441 ttacatgttt taatttataa ggagatgctt aaacatgaaa gaatttaaag aattggaaga
    37501 ttttattgga actcgcttta tctatacgta tgacaatggt tgggaatatg aatggtatgc
    37561 caaaaatgac catactgttg attaccgtat ccatggcgga atggtcaaag gccgttgggt
    37621 aaaagaccaa gaagctgata tcgttaaatt gacggacggc gtattcaaaa tcacttggac
    37681 agagccaaca gggacagacg tggcattgga ctttatgcca aatgaaaaga aacttcacgg
    37741 aatgatcttt ttccctaaat gggttgaaga acatccagaa atcactgtta ctttccaaaa
    37801 cgaacatatc gcagaaatgg aagcagctcg tgaaaaatat gaaacttatc caaaattact
    37861 tgttcctgaa tttgctacga tcacttatat gggtgatgca ggtatagaca atgaagatgt
    37921 tattagcgaa gaaccatacg atggaatgcc agatgatatc cgtagcggaa aatacttcga
    37981 tgaaaattat aaacgagtaa aataaggttg tgaaagagag gttgtgaaat cagcgttctg
    38041 acctgagtat tagagaaaga aacggaaaaa tagttcctca tgttttgccg ttttttgagc
    38101 taataccgag ggtcagctga ttgaacaccg tttatcaaga ggttgtgaaa tcagcgttat
    38161 gctttgagta ataagaacgc tagttagaaa atagcttttc atattttcta ggctagtggg
    38221 cttattaccg agaagcagcc gtttgaacac cgtttatcaa gaggttgtga aatcagcgtt
    38281 ttgacctgag tattagagaa acaaatggaa aaatagttcc tcatattttg ccgttttttg
    38341 agctaatacc gagggtcagc tgattgaaca ccgtttatca aagattgtaa caactgcata
    38401 ttcacacagt taaattagtg acttcaaaat gaaataatgc tcccagcaaa aaattaggct
    38461 gggagcatta tttcgttatt ctgttcatca aaaatcagcg ttttgattcc atttcatcac
    38521 aagttcctcc tcatttactg cttcatcttt ttgctgtgtt tgaattgtaa aaccttgtga
    38581 aagatagaaa tgaatagctg attgattttt ttgatagact gtcaaagtca gtgttttatt
    38641 ggtttctttt atttttttca ttagttgcgt accgattctt tgttgccggt attccctttt
    38701 cacaaagatt cctgcgataa actgatcaat caccccagca aaaccaataa tttcttgatc
    38761 tatttctgct aagtaaatca ctgcatctgg taaagctttc ctaacagatt ctacttgatt
    38821 ctcccaataa gttgatggaa tgaattcatg agaatccaag ttagcatgca gccaaatcga
    38881 tagaacagat tccagttctt tggcagacaa ttttgttgtt gtgcggatca ttttttctct
    38941 ccttacttat tcattgattt taaaataaaa accatttttt tgaagctctt ttacaagttt
    39001 tcgcccctca aaaaagtata tcttctcttg ttttactttt ctaataccaa aagaaacagc
    39061 aactttagga tcatcagctg aaacgattcg ataagttcca ccaggcaatt gatccagctt
    39121 gactttccca tgatggtcgc tttgtactga aaacggcaca ccatttctag taagtgtttt
    39181 tttcttatgt ttcaaaatga atgaactacc atttaccggt ttccctgacg aatcactaat
    39241 aataaatgaa aaatcaagca cttttttact taatctatac tgatagatta gtcgtccgat
    39301 aaacactaac agtatgacta cagctattat cgttcctgca agaataaacc attgccgcat
    39361 ggttctttcc tgatttcctt ttccttcttc tttttttacc gattcgttat aaggaatccg
    39421 atgtcctgtc actagcaaac gatgcgtgtt gatcatatac ggcgtacatg taagcaaggt
    39481 aactaagtct tctccttctg aaatcgttag aacagacgtg tcatctggca gtaccacttg
    39541 tatattttta acttgataag ccaatttctc ccccaaaaca gtcaaaataa acgtgtctcc
    39601 gttttctaaa cgatctaatt cggtaaataa ccgtcgatta ggtaaaccgc tgtgagcaga
    39661 gatcactgaa tgcgtcgaat ttccaccaag cgggaaagag gttccttgca agacgcctgc
    39721 tccattttca aggatcctac cattgagccg atcataaagt gaaatattta attggatctt
    39781 aggtatattg atacttccga tcagccattc ctctaattgg ctggatgttg tactttcatt
    39841 tctgttcttt tcattgaaag gatcagtacc aggattgaat ccttggtcag ttaatcgttg
    39901 ttttttctcc tccattttct ttcgctgcac ttcaatttca gctgcggtcc gtttttgtac
    39961 ttgttcgata cgtttttgat caataaaact atttaaagaa tccacataaa atggatataa
    40021 agcgatcagt aaccctacga cgatacatat taataaaaac aaacgaaaca aaagtcgacg
    40081 agactgtttt tgcttactct gcttcacatg tgtcctccct tgcaagtttt tttgttcttt
    40141 cttttattat atgggttcag gaaaaaaata gaaaatgaaa gccgatgagg ttgtgaaaaa
    40201 gctgtcctgc atcaagtaat aagtacagcc caaaaaaata gctactcata ttttttgttg
    40261 ggttgggctt attaccgcag atgcaagctt ttgaacaccg tttaaggaag aggttgtgaa
    40321 aactgcgtta tgctttgagt aataagagcg ctagtcagaa aacagcttct catattttct
    40381 aggctagtga gcttattacc gagaagcagc tgattgaaca ccgtttaccg agaggtcatg
    40441 aaaaagctac tttgtttcga gtattaagaa aaattctgtt tcaaaacaag gagatgcaac
    40501 aaaacttatg tcgcatcctc tctccttaga actttcactt atgtttttct tgtaaaattc
    40561 ataaatattt tcatgaattt tctaatttct tgcctacctc tcgcatagga tatatgatga
    40621 atttatctaa agtttgcaaa ggagttcgac aattgaaaaa atatggccca tttttagcaa
    40681 tttttttcat tgctttggca tttctcatgc ctcatttatt cacacataaa ctaattttgg
    40741 gtgctgatgc cccattccat tacaatcggt tctatgatgc cgcgcaacaa attaaatatg
    40801 gggatttcca ttattttcct tccctttatg gattccagca aagtggcagg atcgtgaatg
    40861 ccgtttacgg cccatctttc gcttatttac aaggattgat cgtgttgctt gcaggtagct
    40921 ggtttcatta tcagattctt tcaaatttca ttctttatct tatttcagga ttttctatgc
    40981 tgattttact gagatatgtc aatgttcgaa attgggtaag tgtaccagta gccgttttat
    41041 tcatgagtac gtattctatt caatactggt ctatcaacca aggattcact agctggggga
    41101 cttgttttta tcctttatgt atgatcccta ttgctgattt cgtccttcat aaaaagttcc
    41161 ctgttatcaa agtcgctgtc tcagtgggac ttatgacaca gatccattta ctgtcttcga
    41221 tcatgttagc tttgatgtat atcccttttt atctttatta cttttttaac cagactgaaa
    41281 aaggaaaagc actgcttcaa ctgattaagt cggtcttgct ttattttgtt ctgacagcaa
    41341 atatttgggc aggattagct attgtttaca gtgggaataa gattcttcct ccctttgtta
    41401 acagagaaat gagtcaaaag gcgatagatc tggatggcag ttattggctc cattacccta
    41461 aggcctttcc tatattgata ttatcaggtt ttttgctttt tattttcttt caacaaaaga
    41521 acaccttatt ccaaaaaatc gtgttgtaca caagtcttgt tttctttttt ctttctacta
    41581 gcttgattcc atggacgaca ttgatcgaaa aaaatgtgcc ttttattagt ttgatccagt
    41641 ttccatttcg atttttcgtg ccatttacgc tattgcttct actttacctt gccctttcac
    41701 tgaaccagtg gtctgaacaa aagtggttcc gtcttttaag tatatttggc attcttattt
    41761 tttcagcaca gacgatccag aacttatacc agcatctgga aaagtgggaa aatgaaacat
    41821 ttgtcagtcg acatacttat ctattcgata cgccggaaga agcaaggaaa acattcttca
    41881 gcaaagatat gtctaccagc ttgttcgttt tccaaaaaac cacacctgac tatctgccaa
    41941 tctataagga gacaaaagac aataaatacg accggtataa cgaagaaata ctagaaaacg
    42001 aaacaacttt tataaaaaca catccaggcg gtcttttgac aatcaagtgg aaaaacactg
    42061 gggacaaaac tgttcatatc cctgttatcg tctacgatcg gactgtgcta cagcaaaatg
    42121 gaaaaacatt gactgattat gaagtaacag atatcgggac cccaatcgtg aagcagaaaa
    42181 aaggaatcaa cgagctcacg cttcattatc aaacgccaat ctatttctat ttcattcttt
    42241 ctcttacttt gattggctgg tttaccttgc tatgtttatt tatttatcat agatacaagc
    42301 ttttacgtgc ttaggcaaaa gggataaata catccgatca aaaaactctc caaacggtat
    42361 aatcgtttgg agagttttat tttaatcagt ttttttatgt tatatgttat attttttatt
    42421 cgttcacatc taattcgatt tccaccccgt agtgatcact gatgattgga ctatcgatcc
    42481 catcaaaaat ccgttgatat tttttaacct gcattccttc agggacgaaa ataaaatcta
    42541 tacgcaattt ttctgtattt tgttcccaac catctatttt cttttccaca gtagctgctt
    42601 ttcctttttc attcgcgaca acatacgaat cttgaagaag aaggcaactt tctcttacag
    42661 tataataacc ttgagagtct actggattat tgaaatcacc taggaaaaaa agtggtacgc
    42721 gagtttccaa aaagtagttt tccaagtttt tccactcaaa ataaaagcca ctttttttat
    42781 ctgtccacca agaaaagtgg cagctcgaga caaaaatgtc ctgatcatct atctttgttt
    42841 tcccaatcaa gatcttgcgt gtctgataat cttctttctt ttgagattgg gagacggtaa
    42901 ggacctgact ttccaatgtt tttttcgtaa gcagtgcatt tccttcttca tatttatcat
    42961 aaccgatgtg gctcatttcc caactccaaa aatactcttt tccgtgttcc tttaaaagct
    43021 gaactaaacg gtaggcaaaa ttatctttac ggataggtac ttgattcttt acctgacaga
    43081 acttcattag cttcgcaggt tctacttctt cagattccag taattggttg atttcttgta
    43141 aagcaatcac ttcataatct tctaacagga tcttatctgc taattcttgc aattttttct
    43201 ctggttctgg ctcaagccag ctatgcgtgt tgagtgtcat gaatttcatt ggttttctcc
    43261 ttgtcttcat ttcgaaacaa aagggttgtg acaaaatttt tgtcacaacc tctccagcaa
    43321 atagtgttct cctgctaact cctcagtaat aagctcaatg gaccaggagc tattttttgt
    43381 ttggctgttt ttatcacttg gtgcagggca gctttttcac gacctctcca ctaaacagta
    43441 ttcaaactgc tgcttctcgg taataagccc aaccaaacga aaaataggag gagctatttt
    43501 tgtttggctg ttcttatgac ttgatgcagg acagcttttt cacaacctct ccttacattt
    43561 taatggagcc gatcacttta ccagctgtca ctgtacctaa ttgcttgatc actacttctt
    43621 ctactttatc gtttgtaaat acgatcatga tcgttggatc tttttcttcg ttttggatca
    43681 catcaagatc catctcagct aatttcgttc ctgttcctac ttcttggcct tctttgacag
    43741 caatctcaaa agctggctga ttcatttgga ctgtatcgat tcccatatgg atcaaaactt
    43801 cgataccatc tttcgtttcc agcccaattg catgtttgct agggaaaata cttgtcactt
    43861 ttccagcgat cggcgcaaca acttctcttg atgttggaat aacagcaaag ccgtcaccca
    43921 tcattttttg tgagaatacc ggatctttta cttcagataa aggaatcact tctccagaag
    43981 ctgcggaaac tagttcttgt tctataccta agtattgctt actacctgct tgtccatttc
    44041 ctgtagaaac catttctggc tctggaatct ctgctcctga ttgcagcaga tcttcgatat
    44101 ctgatttcaa gacatctgct tttggtccat aaactgcttg gactccattg tctttgacga
    44161 tcaatcccat cgcccctgct cgtttccagg cttcttcagt ccctactttt tctcgatcat
    44221 ttacacttac gcgtaaccga gtcatacaag catcgacatc tttgatattt tcttttcctc
    44281 ccagcaaatg aatgatctgg accacttgct gatctgctgt tcctgttccg ctgttagata
    44341 caacggtttc ttcactgtca ttgtcgtaat ttccgttacg tcccggtgta gcaaagttga
    44401 attttttgat caagaaattt gctaagaaat acgtaacaac accaaaagcg atcacacaca
    44461 acacgaagtt gataagatct ccacctagac ccgctttgat agctaacggt gtccgagtca
    44521 gcaattcgat attcccaaat gaatggaccc gcaatggcaa gatatctgcc atcgcaaatg
    44581 cagccccttg gatcactgaa taaatgacat ataatggcac agcagcaaac ataaacatga
    44641 actccaatgg ttctgttaca cctgttagaa agacagccaa cgctgctgaa aaatacattg
    44701 atttatatct tgctttctta tcaggatcga cattacgata catcgcaaaa gccattccca
    44761 tcaagatacc tgaagaacca atcatttgtc ctactttgaa acgagccggc gtccagttat
    44821 gcaacacaaa ctcatattgc gacatatccc ctgcaccttt aagattcact aaatcagtgg
    44881 cccaagccaa ccataacgga tcttgcccaa atacttgtgt ccccgcctgt gcgcctgata
    44941 aaatctcata cgatccgcca agctgtgtat agttgatagg aatcgtcagc atatgatgca
    45001 atccaaatgg caatagtaaa cgttctaatg ttccaaataa aaacggtgct agaatcggtg
    45061 cgctttcttg tgattctgca atccataaac caaaattatt gattccagcc tgtacgtttg
    45121 gccacaccac agcaagaatc aatgccacga ttgttgacca taagataaca acaaacggaa
    45181 caaagcgctt tccattgaaa aatgacaaag catccggcaa ttttctataa ttatagtact
    45241 tattataagc cattgccccg acaaatccag cgatgatccc tacgaatact cccatattca
    45301 aagctggtgc ttccaataca cttgtaaaaa atcctttaac catgatcttc gtaccgaaca
    45361 gagtgtgggt aaatgcatct tcattcgcaa gcatgtcact tgtgacacca aagatagccc
    45421 cagtgatccg attgattaaa ataaatgaga tcccagctgc aaatgctccg cctgctcttt
    45481 cttttgccca acttcctcct attgctaaag caaataaaat atgaagatta ccaataattg
    45541 cccaaccaat attttcaatt actccgcccg ttgtgacaag cggcgtccaa tcaggattga
    45601 tcaagggaat tgatttccct atactgatca tcaacccagc tgccggcata actgctacca
    45661 caaccattaa agcctttcca aatttttgcc aaaactcaaa actaaatatc tttttcattc
    45721 cgtttagctc ctttacgcaa tcggtttcgt taaacgtatt atagcgtgcg ttttcttttt
    45781 tgtaaatgct ttatttttta tttttctgtc tataccattc tataaaacct ataatacgtg
    45841 tgttcccttc tataattttc tcctaaaaat ttattttttt aagctttttt ctatgaaata
    45901 ttttcttttg tctattgact tgtgcaatcg gttgcgttta ttatgaacgt ataaaacagc
    45961 tcattaggag gagaaaaatg aaacagatca aacgactatt tgctattgat ccatggaaaa
    46021 tcagcacgac acaattagat aaaaaaaatc ttcgactgca agaatcttta acaagtattg
    46081 gaaacggtta tatggggatg cgcgggaact ttgaagaaac ttactcaggg gaccaccatc
    46141 aaggtactta tttggcgggt gtctggtatc ccgataagac acgtgtcggc tggtggaaaa
    46201 atggttatcc agaatacttt ggaaaagtga tcaacgcctt gaactttatc gcgatagata
    46261 tttcgatcaa cgatcagctg atcgacttgg cttctttgga accagaagac ttttactggg
    46321 aattagatat gaaaaatggt gtattatctc gtacattcac aatcactact tctactaata
    46381 aagtacgcat ttccttcgaa cgttttctga gcattgtcaa aaaagaatca gcttatatct
    46441 atttaactat agaaatgctt gaaggtagcg gaacagtcaa actcgtatca aaattggatg
    46501 gaaatgttca aaacgaagac agcaactatg atgaacattt ttggaatgag attgatcgtg
    46561 gaactataga agaaatcggc tatttgacga ctaaaactat tccaaacaac tttgggattg
    46621 aacaatttac tgttactgca gcaatgcgcc atttcgttaa tggaaaatca gtcatccctg
    46681 actatcacga agatacttta gcattacatg gttgcttgtc tgcagaatta tctcaaggtg
    46741 agacatggtc agtggaaaaa gaaattatcg ttcttactag tagagatatt ccagaagcgc
    46801 agcaagcaga aaaagctgtc tcagaattaa cgaaccagac aaacaattat atccaagcaa
    46861 aagaagagca aacagcggct tgggcagaac gatggaagct tgccgatgtg gtcatcgaag
    46921 gagacgaaga agcacaacaa ggtattcgat tcaatttgtt ccaactattc tctacttact
    46981 acggcgaaga tgaacgattg aatatcggcc ccaaaggatt cacaggagaa aaatacggcg
    47041 gagccactta ctgggataca gaagcgtatg ctgtccctct ttatcttgct ttagccaaac
    47101 ctgaagtaac taagaatctg ctgaaatatc ggcataatca actgccgcaa gcaatccaca
    47161 acgctcagca acaaggatta aaaggcgcgc tttatcctat ggtcaccttc actggtgtcg
    47221 aatgccataa cgagtgggaa atcacattcg aggagattca ccgaaatggc gcgattgcct
    47281 atgcaatcta caattatacg aattacaccg gcgatacgga atatttgaaa aatgaaggac
    47341 tggaagtttt agcagaaatc gcacgcttct gggcagaccg tgtccatttt tcaaaacgag
    47401 caaataaata catgatccac ggtgtgaccg gcccaaacga atatgagaac aatatcaata
    47461 acaattggta tacaaatacg atcgcagctt gggtcttgcg ttacacgcga gaaaattatt
    47521 taacttatca aaaagaagct gctatcacta tcagcgaaga agaactagcc aaatggaaag
    47581 atatcgcaga gaacatgtac ttcccagttg acgaagaatt aggaatcttt gtccagcatg
    47641 acacattctt agataaagat ctcatgcctg tatccgatat tcccacaagc gatcttcctc
    47701 tgaatcagca ttggtcttgg gacaaaatcc ttagatcttg cttcatcaag caggcagatg
    47761 tactacaggg gatttatttc ttcaatgata cttttacaaa agaagaaaag caaaaaaact
    47821 ttgatttcta tgaaccaatg actgtccatg agtcttccct ttcgccaagt atccattcga
    47881 ttttagcagc agaattagga atggaaagca aagctgtaga aatgtaccag cgaacagctc
    47941 gcttggattt ggataattac aataacgaca cagaagacgg cttgcacatc acttccatga
    48001 ctggtagctg gttgtcgatt gtccaaggat ttgctcaaat gaaaactgcc catgatcagt
    48061 tgagattcgc accatttctg ccaaaaactt ggaaagctta ttctttccat atcaattatc
    48121 gcggacgttt attgttcatc tcagtcacag atgaacaagt tactttcgag ttattagaag
    48181 gttttcctct agaaatttac gtctatgaac agtcttattc tttagcggat cgtctagaaa
    48241 taacgctaag aaaggaggaa gcaaatgttt aaaggggtat tgtttgattt agacggtgtc
    48301 attaccgata ctgctgaatt ccattatcgt gcttggcgaa agcttggtca ggaaatcggc
    48361 attacgatcg accacacatt caacgaacag ctaaaaggcg tcagccgaga agactcactc
    48421 tctttgcttt tagcttatgg cggaaaagaa cactcttttt caaaggaaga atttgcagaa
    48481 ttagcgaaaa gaaaaaacga ttattattta gagatgatcc agacgatcga accaaaagat
    48541 gtcttccccg gggtcgtgcc tttactggat tctttaaaag aagaaaaaat caagattgct
    48601 cttgcttctg caagtaaaaa cgggccattc ctacttgaaa aaatgggtct tacaccttac
    48661 tttgatgcta ttgccgaccc ggcagaagca acaaacggaa aacctgcgcc agatatcttc
    48721 ctccttgcag caaaagctgt cggactaaaa gcagaagaat gtatcgggat cgaagacgcg
    48781 caagccggta tccaagccat cctctcaagt ggcgctcaac caattggcgt gggacgtgct
    48841 gaagaccttg gagaggagat cccgcttgtc cctgatacga cttttcttac gatcgattac
    48901 ttgaaagaaa agtgtcatga ccatggataa tccagagatc tccgtcgtac cagttccaga
    48961 gactgaaata aagaaaatca cattgaagaa cgactactta gaagtcgttc ttctcaatta
    49021 cggtgcccgc ctgcaccaaa tcttcgcacc agacaaagaa ggaaaatcag aaaatgtttt
    49081 attgtcttac gatcgtttcg aagatgttct aacagacaaa agcttttttg gagcaactgt
    49141 cggacctgta gccggaagga tccgggatgc ttcttggggt gagcatagat tggagaaaaa
    49201 ctgcggatca caccatatcc acggtggcac gaaaggctgg tctttccaat tttgggaagt
    49261 agaagttttc aaaactccat catcaatagg tgtggtcttt tacttaaaag atgaattttc
    49321 tacctatcca ggaccaatca ctgctacgat cacttaccgt ttaacaaaaa acgaattaga
    49381 gatgacaacc tctgctagta gccttgttga aaccatctgc aatccgacca accatgccta
    49441 cttcaacctt tcaggaaatg gaacaagaga catctatgaa catcagttaa cggtcttttt
    49501 agatggtgtg cttgaattag atcaagaaaa actgcctact ggaaattgga caaaaaaaga
    49561 aaaactgcca atcgacttta gaaaatcccc aacactgcaa gagatccttg catgttatcc
    49621 agatggacta gatgatgtat ttcttttgca tcatcctagg ctttccaaaa cttccttaca
    49681 attattcgag aagcattctg gtcgccaaat gactatagca acttctaata aaagtatggt
    49741 attattttct acgactggat tcgaagctga tttctctgtc aatggaaaac agatgcattc
    49801 aaactacgga ttagcaatcg aaccgcaaga aatccctgat atcgttcatt tccccaaatt
    49861 tggatcgatc aacctccatc ctggtcaaga acggatcagt cagacgattt atcggttctc
    49921 tgcacaataa caagagaaag gtgtttttat catggctatt accgtaaaag atgtggcgaa
    49981 aaaagccggt gttgccacct cgaccgtctc tcgtgtcatc aacgatcatc ctagtatttc
    50041 cgagacaaca aagaagaaag tccgaaaaat tatggatgaa ctaggttatg ttcctaacat
    50101 gactgcccgc aatctaggaa aacgcatttc cagtgccatc ggcgtaatct taccccctct
    50161 tgattcaaaa gagcggatcg gtaacccatt ttatcttgag atcatggaag caatcaacga
    50221 agaagcgcgg aattatgata tgactacagc aatcgctact gctaaaaatt ttgaattgct
    50281 gttagagaac gtccagcgta tgcatttaca aaaacaagta gatggtttta ttctagtgta
    50341 ttcagatagt caagatcctg tcattgatta tttataccaa aaccacatcc ctttcacttt
    50401 gatcggacag ccttatcatc atgagaacga aatcgtttat gtagataatg ataaccagct
    50461 tctaggtaaa caagcaacag aatttttgat ccagaatgga cataagaata tcctgtttgt
    50521 gacaaacacg actcatgaaa gtttgtattt cgaacgatat ttcggttatc aaaaagcaat
    50581 gatgatggct ggtcttcctg cgttatccag tgtagtacta gagcatccgg aggattatct
    50641 gtcctttgag aaaacgttga aagaaaacga agccacagca ttggtggtca ttgacgatat
    50701 cttcgccctt cgcacgatgc agttggcgca aatgtacggc tatgtcattc cagagacact
    50761 ctccgtcatt agcttcaaca actcgatctt ctcaacatta acacaccctt accttacaag
    50821 tatcgatatc gatatctccg aattaggacg tgtcgctatg caaaagctgc gcgaattgat
    50881 ccatgaagaa ttgagcagtg gcgtacgttt ggtcatccct catcgcttga tcaaaagaga
    50941 gacagtcgtt cctttagaaa acccatacta aatagctgac tcactaaata cgctgaccat
    51001 ttatcctatg gtcagctttt tatgtaaaag aagatactac ttctaacaca ttgtttatct
    51061 gtaaaaaaga gcgtgggaca aaagtaaaga acacttttgt ctcacgctct aaacacgtat
    51121 aaacggcgga agcagaagca actccttcgg aaataagctg aaattcacaa aaatttaaaa
    51181 agcaattttc ttgagtttct tcttatttct tggagttaaa cgcttctgtc ccagcctctt
    51241 ttttccttcc ttcaattttt tacacgtcga cgattcaata aaaaaccgcc taatattgta
    51301 aagacaggtg caagaatcga gaaaaagacg aatggcaagt aatgcaacgc gctaactttc
    51361 atcgttccca taatgaatgt gccgctgact ccccacggta cgagagaatt gacggtcgca
    51421 cctgaatccg ctagtgttcg tgtcaaatag gtttttgata agccaacgtc atcaaaagaa
    51481 cgcttgaatg tttctcctgg aagaatgatg gacagatact gttctccaac gatcagattg
    51541 attccgatac agctcaaggc agtaaacccg actaaacgag aaggtcggtc catcttttct
    51601 tttaattctt gaacgatggt atcaacgatc agatatttga tcagtaaccc acctaatgcc
    51661 aatgcaagta aaatcaaact agcagaacct aacatactca tgatcccgcc tcgagaaagc
    51721 agttcatcta gctttggatc aggtgtattt gcgacataac cattcatcaa gatgtctgct
    51781 gcttttccga ccgtcaaact tttctcatgg ataaaagaaa gaacgagagc agctgtcgat
    51841 cccgctagta atgtagggat agccggaatt tttttgattg cccccccaaa taaaataact
    51901 agtggaatca atgtccaagg agagatccaa aagctcgctt tcatgttttg gatcatttca
    51961 ttgatactag ctagatcggc tgaatgactg ttatgtccta atatcgtata aaaaatcaac
    52021 gaaaggacga atgctgggaa atccgtataa cgcataccag cgatatgttc gaatagatta
    52081 actcctccaa tacccgctgc caagttggtc gtatctgata aaggcgagca gttattccct
    52141 aaaaaggcac cggaaacgac tgcgcctgta gtcattgcat cattgaatcc aagtaaatga
    52201 ccgatcccca aaaaagcaat tccaatcgtc gaaatcgttg taaatgaact gccgacagtc
    52261 actccgacaa ttgcacaaac aagaaatgct gtcggtaaaa agaaacgcac agaaacaagt
    52321 ttaaatccat aaaccataat cgtagggatg gttccagata aaatccatga agcaactaag
    52381 acaccaatca aaagaaaaat caggattgga atgatccctg gagtgattcc tttgatgatc
    52441 ccgtcatgga tctcatccca tgtgaacttt ttaaatctgc cataaaacaa caaacacatg
    52501 aaagctgcaa ggatcgggat atgtggcgag agctggaatc cgatgatcag tactcctagg
    52561 agtgccaaca acacgatcaa tacagcgata ctttctttta aagaaatatt tttattcatc
    52621 gtcgatctcc tatttcaaat gaaaaggccc tgccatgaaa aaactattca cttggcagga
    52681 cctcctacta aatcagcgac aaaacgtcgg ctgaaagact tatttttttt atttttcttc
    52741 taaacttttt tctacctcaa tcccccaaga atctttcttg aagtgattga tgatcaatgg
    52801 aataacgaac atgaccactg tgatagctgc tactaataat gcatcactca atgtcgctgt
    52861 acctaatgta gcaaatacga caacgatgat agagaagatc agcatgcagc tgactaccca
    52921 agctaatccg tttccgtttt taccaattcg gtaaggacgt tcgaattctg gatgtttctt
    52981 acgcatttta agcaatgaaa cagcaatcaa aatgtacacg atacaataaa taacagttgt
    53041 agcatttgta agtgtcaaga atacagcact tacgtcatcc attacaccat aaagcaaagc
    53101 aaataatgaa ataacgattg attgtgttag aacgacattt ctagatacgc cgtatttgtt
    53161 ttctttatgg aatccgaatt ttggtggaag gaatccttca cgagctactt ggatgattgt
    53221 ttttgatggt ccagtaaccc aagctgataa ttgtaaaagc acaccaataa ataccatgaa
    53281 gctgaagata tttccgatca cttctggtaa gcctaggatt tgacagtcaa tcaagatagg
    53341 ctgcgtgata tttgctaatt ccatttttcc atctggtacg acatttgcta caagcatcgc
    53401 attgatgaca ttcaataaaa ctaatccgat caaagcgata aatacgcctt ttgtataatt
    53461 ttttgttgcg tctttcaaac gaggcatgta aacagaactc atttcgatcc caacaaaaat
    53521 aaatgtgata ccagctaagt atttcaatga atcaatatgt tccaagtcag ggaaagcttt
    53581 tgaccatgaa aagtcaccta gatagccgtt tgagactagt ccgactttga aggctgccaa
    53641 aacacctaaa acgaacatga tgactactgg gatatatacc cctagccata caccgatgtt
    53701 cccgccgact tttgccatat cgaatttcaa gttcaagatc gtgatgatcc agtagataac
    53761 taaaatacaa cctaaaacga accagtgatt attgccaagt tctacattac caaatgtgtt
    53821 tcctaacaat ggtcctaaag tagaagcgac cattaccata cctgggaaca tttgcaccca
    53881 taatagccat gcaacgacaa atccccattt gctgcccagt gctgttttta cccataactg
    53941 aggaccgcct tcttgaggaa gcatcgtgga aagctcaccg gaaaccattg atatcggacc
    54001 ggcaaaaaac aaaactgcaa aaattgaata aaaaatcaat gtccaaccga ctgcggctaa
    54061 cgtaggaata ctccgaaccg ttgcacacag tgccattgtc agaccgataa aggtcccaag
    54121 acccatttta cttacttcag atttactcac cgttgacact ctccttgatt ttttggataa
    54181 aataacggtg tctttgaagt agtttcagca ataaacgaaa caattcagcg aatatttgag
    54241 gggattcttt gagtctattg cttgaagggt ttcttcaaag acaacctcaa aacaaaggga
    54301 tcgaagagct taggtctccc cttgctcttc agatccaaac atgcgtcaga aacagttagt
    54361 attcaagctg ttttctaaca tgattacctt gcttattttg cttcgcttgc caataattgg
    54421 tctgcgtaga ttttttccaa tttttcttga agagctgctt tgattttttc agcatattct
    54481 tcaaagtgtt cttctttgtt catgtatggt gtcattacgc tggcacgcaa tacagttact
    54541 tttccggcac ggttccattc ttcatctgag aatcctagtt ggttcacaaa ttgtaatggg
    54601 ctgttgccat agtctgggat agcaaagtca gtatgtgaag ttaagaactc attaccgtaa
    54661 atgcttcctt tcacgtatga agaataatca tatacgtcat ggttcaattt attcattgca
    54721 actaagtcat cgttgccttt ttctttgaat acatagtcta ccatattgaa atctggataa
    54781 gttaatggat gaacttcgat ttctttgtct cctactttaa atgacaagtc attcaagaag
    54841 ttgtagaaac gatgtgcacc ttcgattgat gcacccatta atttaccata tcctgtgaca
    54901 ttcaaaggta agacatggtg tgctgcccaa acgctggctg ctgttgcccc tgcttttgaa
    54961 ccttccaaga tataagcccc aagtaatgct gggatatctg cgcctttttc aaatacatat
    55021 gtcgcaaagt aagagataac gtctctcatg cggatatctt tgatgacgat accaccggca
    55081 gaatatggaa catagcccat tttatgtggg tcaatcgtta cagattctgc ttcttcgatt
    55141 gctttatatg cactgtgtac ttcttctaag atataatctt tgttttctgt aaagacattg
    55201 tatttgtaat gaacatcttt taaatcttca aatggaatga agttgttgtc ttcgtctaag
    55261 aaaatcgcac ggccgtatcc gccataagca gcatctacgt gtaagtagaa gtaaataccg
    55321 tctttttcta atacgcgacg caatgcaacg attttatcga taccgtcgat tgctccttct
    55381 tctgttgatc ctacaacacc gactacacct aggattggtg ttttttcagc tgctagacca
    55441 cgaacgattt tttcaagttc gttgatgtcc atgcgatagt tgtggtcaac tggcactgga
    55501 atcacttggt ctaaaccgat accgatgatg tcggcagctt ttagccatga atagtgttta
    55561 gtttgtggaa ccaaccattt tcctaatttt tctaaatgtt ttccgctacg tgctgagtga
    55621 gctttgattt cgtcgatttt ttctgggacg ctgtctaaca gattcatgat ttcttctgtt
    55681 gacaagttca ttaattccca atcgcttttt ccagcaacta gttcaggtgt cacttctttc
    55741 attgcaagcg gcaatgattt gatgttgcgg gcataccaaa gtccttctaa gtttgctaaa
    55801 gaaccatcag caacgatgtg gccccagcca tctttatagc tcattaattt tgcaaattcc
    55861 attcctactt cttcttccat ttggcttgtt gcaggagaag attcataggc aacgttatta
    55921 ccattccata gcatagcaaa attgtacgct agtaaagatg gcatcaatgt ttcagagttc
    55981 atatgacccc agtaacgtcc ggcattatgc caaggaacgg aatgggtacg catccgcgct
    56041 gaaatttctg atagaacatc ctttgtttta ttcacagtgt gttcaaaact agcactgctt
    56101 ttttcttctg gcgtaataat aggcatgtct tgaggcatgt agttttgacg ccagcctaaa
    56161 tgttcatcca ccaattcatt caacaaagct ttatagattt ggccgttctc agctttgtcc
    56221 ccaatgaata gggcatttag gtttagatct ttcgacaatg attcactcat agtgaaaccc
    56281 tcctaaaaaa atatttaaat tttgtcgagc cctacattac acctgttctt ctattctgta
    56341 aactaagttt tggcctgatt tttaatcata ttttaatatg tatcaattat ccgaattgtt
    56401 cacacttttg aaataattaa aaaaaacgag ggaatttaca aatgtaaact ccctcgcaaa
    56461 gattgtatta acgacaacca gcgttctatt ttaggatggg tttcaaaatt ggaatgttgt
    56521 aatagtattt ccctttttca aggtaaaaac ttactaaatt ctaatcaatt actttggcca
    56581 aaaagtaatt tttcttacct tttcgaataa ccacaaactt accatcgaat tcatcggatg
    56641 gatttatgac aaaatttaaa tcggtaacgc ggtcaccgtt aatacttata gctccgtttg
    56701 aaacatcttc acgtgcttga cgtttagatg gttcaatttt tgtggaaaca agcagttcca
    56761 cgatattttc aggtgtgctt gaaatttcaa cgttcggcat tttaccgaat ccttgcgcga
    56821 tttcttccgc atttagttct ttaatatttc cagagaacaa tgcttgcgtt attttttgcg
    56881 cttctttcaa gtcttcttca ctatgcacaa atcttgtaac ttcttctgcc aaacggcgtt
    56941 gtgcttcacg tttctcaggt tctgtctcta cttttttcgc taagtcttcg atttcttctt
    57001 gagacaagaa agtaaagaat ttcaagtatt tcactacatc gcgatcatct tgattcaacc
    57061 agaattggta gaactcaaat ggtgaagttt tcttcggatc aagccaaacg gcaccgcctg
    57121 cagtttttcc gaatttcgtt ccgtcagcct tcagcatcaa tgggatcgtt aaaccgaacg
    57181 cttctacttc tggtccttct aattttcgga ttaggtctaa tccagctgtg atatttcccc
    57241 attggtctgc tccaccgatt tgcaattgca catcgtatgt tttatgcaga tgcaaaaagt
    57301 cgatagattg cagaatttgg taagtaaact ctgtaaatga gattcctact tccaaacggc
    57361 tggcaacgat atcttttgct aacatcgtgt tgatgttgaa gtttttaccg tagtctctca
    57421 aaaattcaag taaagagatt tttgacaacc agtcgtagtt gttcacaaat gtgatatttg
    57481 catctttacc aaacaatttt ctcatttgat tggaaagtgc gtccacgtta tgttgcactt
    57541 gttccatcgt ttgcaacaca cgttctgttt tgcggccgct tgggtcgccg atcgttcctg
    57601 ttccgccgcc aatcagaata tatggatgat ggcctgccaa ttggaatctt ttcatcatca
    57661 taaatggaat caaatgtccg atatgcatcg aatcaccagt tggatcaacg ccgcaataaa
    57721 gtgaaatgct cttttcttca acgagctcgc gaagtctttc ttcgtttgtt tgctgattaa
    57781 tggcgtcgcg ccaagttaat tcatcaataa tattcatttt actgacctcc gtgtagtttt
    57841 tttatgaaag caggaacctg ctagcatgtg taattaatta tttcttcagg ataggatagg
    57901 aaataaaaaa agtcccttgg catataagcc aagggacgaa atctcgcggt accacccaat
    57961 ttgtatgtct agatatccaa catacctctc gaacatttaa cgctgtcgcg tggcaatcac
    58021 tgccaaagac tcctgaaatg taattcgctt taaagcatgt actggtttgc agcgaccacc
    58081 agctttctga aacattggct ttaacaacta cttcgtttcg atcttcgtct tgttattatt
    58141 aatatgtgtc tatcatagag cgcaactttt tctttgtcaa cagaaaaagg atattttttt
    58201 taaattcctc attttttctt ttttgaatcc gctctaattc cgaacatttt attttcaatt
    58261 attgttaaaa gaaataactt tttctgtatt ttttctgcaa tttatcgaga agtcacttta
    58321 ctttgcctct tattctttct tcgttacaat aaggaatggg acaagtaaaa aaatgaaaaa
    58381 gcaagggacg aggtatatgg aaaaaaagca aaaaattatt ttaggagcca cttctgcttt
    58441 aactatcgtt attttaattc taagtttgtt ttttgtcatc cgatcatcta caaaaaaaga
    58501 accaatcatt cctgaaaaaa aaataacaga aaaagcaaaa gaaccgacag atgataagca
    58561 acaactagtt actcaagcaa aaaatctggc tcaaagctac gattatgacc aagcgattgc
    58621 gttattggaa aaagatagta agaacggatc tactgaaaca caacagcttc ttcaaacact
    58681 aaaaaaggag aaagaacaac tgatcgactg gtctgatcct actcagatct ctcatgtctt
    58741 tttccacagc ttgatcgtgg acccgcaaaa agccttccac tctcaacagg cacaaggata
    58801 caaagattat atggtgactg ttgaagaatt caaccgttcg atcgaacaac tttatcaaaa
    58861 tgattttgtt ttagttaatt taaatgattt gatccaaaaa gacgaacaag gaaatctttc
    58921 ctttaacggg cttgcgctac ctgaaggaaa aaaaccattg gttctttcac aagatgatgt
    58981 cagctattat gaatatatgg ataattccgg ttttcctagc cgattggtcg tatcaaataa
    59041 caatgaaatc aaaaatgtct ataaagagaa agataaagaa gaaatcggtg actttgacat
    59101 ggttcctctg atcgatgcct ttgtcaagaa acatcctgat ttttcttatc gaggcgcaaa
    59161 aggtaccttg gccctgacag gttacaacgg agttttaggt tatcgaacat caaaatctca
    59221 atatggagac aatgaaaaaa caaaaaaaga gattaaagaa gctaaaaaag tggcggaaca
    59281 gttgaagaaa gacggctgga gctttgcttc ccatacatgg ggtcacttga atatgactca
    59341 agcttcacta gaagatgtaa aaaaagacaa tgaattgtgg cagcaagaag ttgcacctat
    59401 cctaggaaaa acaaatattc tgatttatcc atttggtgca gacatcagcg actggcagcc
    59461 ttattcagca gaaaatcaaa aatttgctta cttgaagagt caaggtttcg atattttctg
    59521 taatgtcgat gcttctacac cagcttgggg acaattaggt acggatttct atcgaaatgc
    59581 tcggatcaat atcgatggca ttcgttttca atctgattta gacggaaaaa atcctattct
    59641 tgatccattc atcaatgtcc atgatgtcta tgacaaagaa gcacgcgaat ctgcttgatt
    59701 ttacgtcatt tatgaaccaa aaaagccgaa ctgacatttt atcgtcagtt tcggcttttt
    59761 tatgctttat ttcttcttcg gtgcgattcc cttgatcagg acttctgtcc attctgctgt
    59821 atatggaagc agttttgcgt atatctccgg cttagaaatt ccttctacca ttgcttgaaa
    59881 atagaggatc aagaactcat ccgaatattt caaatccact tttccttctt tccgccctcg
    59941 atggaaaaga tcaagcatga tactgaaact ttcttgcgta tacgcatcca tcgcgtcaga
    60001 aaattctttt ttttcttttt ttgtatagta tgtcatcaaa tcgatataaa attgtttgtt
    60061 cactcgattg agataacgga ttttgttttc actcatggca atcagtgttt cttcaaatgg
    60121 aagattttcc gcaatcaacg ctttcgcttc ttgccctaaa tcattcataa atgcaaaaaa
    60181 tacttcataa atcagccctt ccttgctgcc aaaatattta aaaatcgtcg ctttacttac
    60241 ctgagcgttc tcagctacct tatccattgt cactccagcg gttcctatgt ctgtgttcat
    60301 caagtcaaaa gctgcttgta aaatctcttt ctttttctct tgtgctctct tttcaaaacc
    60361 gttcatttgt agagcctccc gtaattttaa acttttataa tatatatagt tcaaatttat
    60421 tctgaggttt attataacta tctattgaga aagtatcaag ctag
//
LOCUS       DAJSBL010000014        60192 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.014, whole genome shotgun sequence.
ACCESSION   DAJSBL010000014 DAJSBL010000000
VERSION     DAJSBL010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 60192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60192
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.014"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(114..473)
                     /gene="rplT"
                     /locus_tag="ONT85_000964"
     CDS             complement(114..473)
                     /gene="rplT"
                     /locus_tag="ONT85_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HCR2377958.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            complement(526..726)
                     /gene="rpmI"
                     /locus_tag="ONT85_000965"
     CDS             complement(526..726)
                     /gene="rpmI"
                     /locus_tag="ONT85_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HCR2377959.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            complement(798..1322)
                     /gene="infC"
                     /locus_tag="ONT85_000966"
     CDS             complement(798..1322)
                     /gene="infC"
                     /locus_tag="ONT85_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HCR2377960.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     misc_feature    complement(1366..1491)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            1642..2274
                     /locus_tag="ONT85_000967"
     CDS             1642..2274
                     /locus_tag="ONT85_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HCR2377961.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            complement(2276..3181)
                     /locus_tag="ONT85_000968"
     CDS             complement(2276..3181)
                     /locus_tag="ONT85_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HCR2377962.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(3266..3925)
                     /locus_tag="ONT85_000969"
     CDS             complement(3266..3925)
                     /locus_tag="ONT85_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HCR2377963.1"
                     /translation="MSVIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFY
                     ANPEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEV
                     RVYDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYD
                     YYKELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVA
                     I"
     gene            4241..5185
                     /gene="mvk"
                     /locus_tag="ONT85_000970"
     CDS             4241..5185
                     /gene="mvk"
                     /locus_tag="ONT85_000970"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HCR2377964.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            5178..6155
                     /gene="mvaD"
                     /locus_tag="ONT85_000971"
     CDS             5178..6155
                     /gene="mvaD"
                     /locus_tag="ONT85_000971"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HCR2377965.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            6168..7253
                     /locus_tag="ONT85_000972"
     CDS             6168..7253
                     /locus_tag="ONT85_000972"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HCR2377966.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            7250..8314
                     /locus_tag="ONT85_000973"
     CDS             7250..8314
                     /locus_tag="ONT85_000973"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HCR2377967.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(8563..9366)
                     /locus_tag="ONT85_000974"
     CDS             complement(8563..9366)
                     /locus_tag="ONT85_000974"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HCR2377968.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(9386..10228)
                     /gene="kduI"
                     /locus_tag="ONT85_000975"
     CDS             complement(9386..10228)
                     /gene="kduI"
                     /locus_tag="ONT85_000975"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HCR2377969.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            complement(10244..11251)
                     /locus_tag="ONT85_000976"
     CDS             complement(10244..11251)
                     /locus_tag="ONT85_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HCR2377970.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            complement(11258..11902)
                     /locus_tag="ONT85_000977"
     CDS             complement(11258..11902)
                     /locus_tag="ONT85_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HCR2377971.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            complement(12002..12760)
                     /locus_tag="ONT85_000978"
     CDS             complement(12002..12760)
                     /locus_tag="ONT85_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HCR2377972.1"
                     /translation="MENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVL
                     KILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETI
                     HLGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLE
                     KHPLIPYTEYTITNALRLKKEIKTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAF
                     SVSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
     gene            complement(12887..14038)
                     /gene="fucO"
                     /locus_tag="ONT85_000979"
     CDS             complement(12887..14038)
                     /gene="fucO"
                     /locus_tag="ONT85_000979"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HCR2377973.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEASVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
     gene            complement(14120..14431)
                     /gene="rhaM"
                     /locus_tag="ONT85_000980"
     CDS             complement(14120..14431)
                     /gene="rhaM"
                     /locus_tag="ONT85_000980"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HCR2377974.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            complement(14433..15278)
                     /gene="rhaD"
                     /locus_tag="ONT85_000981"
     CDS             complement(14433..15278)
                     /gene="rhaD"
                     /locus_tag="ONT85_000981"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HCR2377975.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            complement(15292..16548)
                     /gene="rhaA"
                     /locus_tag="ONT85_000982"
     CDS             complement(15292..16548)
                     /gene="rhaA"
                     /locus_tag="ONT85_000982"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HCR2377976.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLKEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            complement(16545..18005)
                     /gene="rhaB"
                     /locus_tag="ONT85_000983"
     CDS             complement(16545..18005)
                     /gene="rhaB"
                     /locus_tag="ONT85_000983"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HCR2377977.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            18158..19027
                     /locus_tag="ONT85_000984"
     CDS             18158..19027
                     /locus_tag="ONT85_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HCR2377978.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRKFS"
     gene            complement(19153..20052)
                     /locus_tag="ONT85_000985"
     CDS             complement(19153..20052)
                     /locus_tag="ONT85_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR2377979.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
     gene            complement(20217..21041)
                     /locus_tag="ONT85_000986"
     CDS             complement(20217..21041)
                     /locus_tag="ONT85_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HCR2377980.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPEIP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            complement(21052..22347)
                     /locus_tag="ONT85_000987"
     CDS             complement(21052..22347)
                     /locus_tag="ONT85_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HCR2377981.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            complement(22363..22842)
                     /locus_tag="ONT85_000988"
     CDS             complement(22363..22842)
                     /locus_tag="ONT85_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HCR2377982.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            complement(22848..23876)
                     /locus_tag="ONT85_000989"
     CDS             complement(22848..23876)
                     /locus_tag="ONT85_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HCR2377983.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLKGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            complement(23893..24999)
                     /locus_tag="ONT85_000990"
     CDS             complement(23893..24999)
                     /locus_tag="ONT85_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HCR2377984.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            complement(25228..27825)
                     /gene="adhE"
                     /locus_tag="ONT85_000991"
                     /gene_synonym="adhC"
     CDS             complement(25228..27825)
                     /gene="adhE"
                     /locus_tag="ONT85_000991"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HCR2377985.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            complement(28130..28510)
                     /gene="yajC"
                     /locus_tag="ONT85_000992"
     CDS             complement(28130..28510)
                     /gene="yajC"
                     /locus_tag="ONT85_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HCR2377986.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(28607..29752)
                     /gene="tgt"
                     /locus_tag="ONT85_000993"
     CDS             complement(28607..29752)
                     /gene="tgt"
                     /locus_tag="ONT85_000993"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HCR2377987.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            29970..30668
                     /locus_tag="ONT85_000994"
     CDS             29970..30668
                     /locus_tag="ONT85_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HCR2377988.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(30836..33475)
                     /locus_tag="ONT85_000995"
     CDS             complement(30836..33475)
                     /locus_tag="ONT85_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HCR2377989.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(34094..35125)
                     /gene="queA"
                     /locus_tag="ONT85_000996"
     CDS             complement(34094..35125)
                     /gene="queA"
                     /locus_tag="ONT85_000996"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HCR2377990.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            35318..36493
                     /locus_tag="ONT85_000997"
     CDS             35318..36493
                     /locus_tag="ONT85_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HCR2377991.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEAIKLASRIAIMSEGRVIQFD
                     TPENILRHPANDFVEELIGEDRLLQAKPNATRVGEVMLNTAITITPEKSLQEAIKLMR
                     EKRVDTLLVTDNSNVLKGFIDVETLNKKRGKVSSVGDILNKHVFYVKESAYLRDTLQR
                     ILKRGLKYVPVVDEQNKVVGILTRASLVDIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            36497..37138
                     /locus_tag="ONT85_000998"
     CDS             36497..37138
                     /locus_tag="ONT85_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR2377992.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            37135..38052
                     /locus_tag="ONT85_000999"
     CDS             37135..38052
                     /locus_tag="ONT85_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR2377993.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYSFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            38049..38720
                     /locus_tag="ONT85_001000"
     CDS             38049..38720
                     /locus_tag="ONT85_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR2377994.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(39261..39749)
                     /locus_tag="ONT85_001001"
     CDS             complement(39261..39749)
                     /locus_tag="ONT85_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HCR2377995.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(39950..40216)
                     /locus_tag="ONT85_001002"
     CDS             complement(39950..40216)
                     /locus_tag="ONT85_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HCR2377996.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(40216..40479)
                     /locus_tag="ONT85_001003"
     CDS             complement(40216..40479)
                     /locus_tag="ONT85_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HCR2377997.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            40955..41140
                     /locus_tag="ONT85_001004"
     CDS             40955..41140
                     /locus_tag="ONT85_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR2377998.1"
                     /translation="MALSYGNKKWSLDEAEIPTYEDTWHLRVEVKEEAENIGGCTLFG
                     KGFGNYDQDIKIKMFYS"
     gene            complement(41282..43105)
                     /locus_tag="ONT85_001005"
     CDS             complement(41282..43105)
                     /locus_tag="ONT85_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HCR2377999.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(43703..46408)
                     /locus_tag="ONT85_001006"
     CDS             complement(43703..46408)
                     /locus_tag="ONT85_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HCR2378000.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(46714..47661)
                     /locus_tag="ONT85_001007"
     CDS             complement(46714..47661)
                     /locus_tag="ONT85_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HCR2378001.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            47851..48120
                     /gene="rpsN"
                     /locus_tag="ONT85_001008"
     CDS             47851..48120
                     /gene="rpsN"
                     /locus_tag="ONT85_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HCR2378002.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(48296..48658)
                     /locus_tag="ONT85_001009"
     CDS             complement(48296..48658)
                     /locus_tag="ONT85_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HCR2378003.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(48669..49790)
                     /locus_tag="ONT85_001010"
     CDS             complement(48669..49790)
                     /locus_tag="ONT85_001010"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HCR2378004.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(49806..50156)
                     /locus_tag="ONT85_001011"
     CDS             complement(49806..50156)
                     /locus_tag="ONT85_001011"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HCR2378005.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(50286..51797)
                     /locus_tag="ONT85_001012"
     CDS             complement(50286..51797)
                     /locus_tag="ONT85_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCR2378006.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(52119..53471)
                     /locus_tag="ONT85_001013"
     CDS             complement(52119..53471)
                     /locus_tag="ONT85_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HCR2378007.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(53624..54700)
                     /locus_tag="ONT85_001014"
     CDS             complement(53624..54700)
                     /locus_tag="ONT85_001014"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HCR2378008.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            54896..56641
                     /locus_tag="ONT85_001015"
     CDS             54896..56641
                     /locus_tag="ONT85_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HCR2378009.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(56921..57358)
                     /locus_tag="ONT85_001016"
     CDS             complement(56921..57358)
                     /locus_tag="ONT85_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HCR2378010.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(57342..58715)
                     /locus_tag="ONT85_001017"
     CDS             complement(57342..58715)
                     /locus_tag="ONT85_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2378011.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(59037..59525)
                     /locus_tag="ONT85_001018"
     CDS             complement(59037..59525)
                     /locus_tag="ONT85_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR2378012.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEVFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(59547..60149)
                     /locus_tag="ONT85_001019"
     CDS             complement(59547..60149)
                     /locus_tag="ONT85_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HCR2378013.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT        17104 a        13016 c        10989 g        19083 t
ORIGIN      
        1 tattttagac ttatacgtta ttgtcctgtt tcatttgatg gaggattgta aataaaaaaa
       61 gaggaaagaa cataactttt cgttatcttc tttcctcttt ataattgttt agcttattta
      121 gccaaagctt cttttgcttg gtcagccaat gcagtaaatg ctgctgcatc gttaacagcc
      181 aagtcagcta acattttacg gttgatgtcg atttcagcaa ctttcaaacc gtgcattaat
      241 tttgagtagc tcaagccatt catacgagct gctgcgttga tacgagcaat ccacaattta
      301 cggaagtcgc gtttcttttg acgacgatcg cgatatgcgt agttgtatga attcattact
      361 tgttcttttg ctgttttaaa taaagtgtgt tttgaaccgt agtaaccttt tgctaatttt
      421 agcactcttt tacgacgttt acgagttact gttccacctt taacacgtgc catgtataat
      481 tcctcctaat aaaaataaat cttcagattc tgttatttgt acttcttatt tcatttttgc
      541 tagttgttgg cgaatacgtt tgaagtcgcc actagaaacc atgcttgctt tacgcaattg
      601 acggcgttgt ttttttgttt tgccgtggaa acggtgactt gtaaacgcgc ggaagcgttt
      661 caatccgcct ttaccagtac gtttgaaacg ttttgctgat ccgcggtgtg ttttttgttt
      721 tggcatgact atatttcctc ctcaaaatct ttcgttatcc gactatcggc tgtccaacag
      781 ccagttagtc attcaaatta cttactgtcg tttttcggtg ccagcgtcaa gaacatgctg
      841 cgtccgtcca ttttcgcttt ttgttccact gttgcaatat ccgcagtttc ttcagctaag
      901 cgattcaaga ctttttgacc aatttcttta tgggtaatgg cacggccttt gaaacggata
      961 gaagctttca ctttatcgcc cttttccaag aacttacgtg cattacgaag ttttgtattg
     1021 aagtcattca agtcgatcgt aggacttaag cgaacttctt ttacattgat cactttttgt
     1081 tttttacgcg cttcgcggtc tttcttttgt tgttcgaaac ggtatttacc gtagtccatg
     1141 attcgcgcaa ctggtggttt cgcaccaggg gcaactaaca caagatccaa gtttgcttgt
     1201 tctgcaatat tcaatgcttc tgcttttgtc ttaactccta attgctcacc atctgaaccg
     1261 atcaatcgta actctcgtgc acgaatgccg tcgttaacca tcatatcctt tgctatggtc
     1321 attcacctcc aaatattatg agagaaagaa aagcttaccg aataaacggc aataaaaaaa
     1381 cgagttctct tacagaaccc gcgcgtactt cacctaatag aaccagtgaa aactatctag
     1441 cccagtgaca ttatcaacta aggcgagaag cgggtgcttc tgcttttatt tctcaaccat
     1501 catagtatag caaaaactaa tctttttgtc aatgtttttc atacagtttg tcttcctttt
     1561 ctgaaaatga ttagattagc agactttcta gcagtgattc ccgcttcttt gctatactaa
     1621 agaaaaacga ggtgaaccca catgaaaaaa atcgaaccta ttccaccttc tcttcctctt
     1681 cttacaaagg gagagtttta tttagaagat gaggagatct tctctggtct tctgatcaac
     1741 gggggtgacc aaagctatct cgatgccaaa aatatggtat taagagagag tcatatccaa
     1801 aaacttacga tgcaaaaaac agttttacgt cattttgaat gcagtaatgt catttttgaa
     1861 aaatgtgatt tttccaatct ggagtggatt ggtggcagtt tccaccaagt cgtgtttcat
     1921 caatgtaaat taacaggtac gaattttgcc gaaagttatc ttcgagactg tactttcact
     1981 gattgtattg cttcgatggc ttcctttagt agtacaaact tgaaagctgt ctccttcgat
     2041 cattgtcaat tagaagacag cgaattttat gaggtcactt ggaaaaacct ttttttgtct
     2101 gacaatcagt tgaccggttc gaactggttt cgaacttctc tgaagggatt agactttacg
     2161 actaatttct tcacagcaat cgctttatct caagattctt tggcgggact aatcgtcaac
     2221 caagagcaag cacttgtgat cgcccaagcc cttggtttgc agatcaaaga ctagatcaac
     2281 gaatccgccg ccacagtgca ataagcatcc aaatagataa gaccaaactg atgatcaagg
     2341 cgattcccca tccgaaaacg gaatgggtaa agggtaactg gagattcatt ccgaagaatc
     2401 ctgtcacaat agttggaacg gtcaagatca gcgaccagac cgtcaaaaat ttcatcgtgt
     2461 cattcaagtt gttattcagc aaattattat aagtgccaga gagctggtct aaaatctgcg
     2521 aagctaagtt cgtcatttca actgcttgtc gtgcttcgat cagcgcatcg tccaactctt
     2581 ctttttcctt ttctgacaac ttgcgataaa tagccaatgc cttgatttgt tctaaaagta
     2641 ctacattttg ttttgttcct gtaactaagt aaaccaaacc gatctctaaa tctgaaagtt
     2701 gcagcagatt cttgttcgtt gttttttctc ttaatttcag attaagccgc tgacgttcac
     2761 tattcacttc ctcgattaaa ggaaaaaagg aatcagaaat caaaaacaat gtacgaaaca
     2821 ataaactgta tacactcatg tctggcattc gttccaaaag cgatcgaatt agtcgaatca
     2881 cgtactcggt attcggcgtg gtaaatgtaa acaaaccgtc ttcttttaaa ataaaagcga
     2941 taggactagt ttcataatga ttatcgactt tttgctggtg cggaacattt gatacaagca
     3001 gtaacgcttc atcaaaagaa tcgtactcta cgcgcgcccg ttcattttta tctagagaat
     3061 aagtcagcat ctcgtcacta atgccatatt cttcttttaa gtttttaaca gctagcacat
     3121 cgttttgctg tacattgatc caaaaattac ggtcttgatt aaaatagtgt ttttcccgca
     3181 tactctatcc tcctgacatt tgtttccagt ataaagaaaa aagtcgctca aataaaggtt
     3241 taccctttat ttgagcaact gtaaatcaaa tggctacttt ggtttggcga atctctgcta
     3301 atttttcttc gatcattttc aagacttctt cttttgcttg aggatcttcc acaaaattat
     3361 agcggtctcc atcgatttgg atttttggac tttctttgta ttcttcatac cattgatcgt
     3421 aacgtctgtt caattctttg taataatcgt atagacttgg atcaaaggac aattgttcat
     3481 acgaacgacc acgtttttcg atacgctcca acatcgtatc aaaagataca cgaatatgaa
     3541 ctaataaatc cggatgtttt ttatgggcag catagggaag ttcttccatc atgttttcca
     3601 gcagttcatc atacacacga acttctgttt ctgttgctct tcctagatct gcatttagat
     3661 ggaaaagcag tgaatcttca taaatcgaac ggtccaatac attattgtca tctttcattg
     3721 cttgtttgat gcttgcaaaa cgtttattca aaaaataaat ttgcagcaaa aaagcatact
     3781 gttcagggtt tgcataaaat aagggcaaga cttcattatc atcaatcgat tcataaaatg
     3841 cctgactatc aaagtgttca gccatcattt ctgttaaact tgattttccg gcaccaattg
     3901 tacctgctaa aacgatcacg ctcatttacc tccagctcac ttcatcacta aatattgtgt
     3961 ttaactccaa cctttttaaa tataccccca atatctagta gtgtcaattg tatttttttc
     4021 tagtcaaaga agaaaaaaag tcaaaaaatt cttaagaaaa aagtgttttt tctttagaaa
     4081 acgtattctt agttttactt ctgagatggt tttagtaaaa taaaggagga atgttttttg
     4141 agacccttag tggacaaaaa aattcgtgaa gcagcgcaac tcgttttggg cgctttattt
     4201 tttgcttaaa aatcactttt acgtgagaag gagttagatt atggcaaact atggccaagg
     4261 agaatcaagc ggaaagatca tattgatggg cgagcacgcg gttgtttatg gagaaccagc
     4321 gattgccttt cctttctatg caacaaaagt caccgcattc cttgaagagc tggatgcaat
     4381 ggacgatcaa ctggtttctt cctactattc aggaaattta gccgaagctc ctcatgcatt
     4441 aaaaaatatc aaaaaattat tcattcactt aaaaaaacag catgacatcc aaaaaaactt
     4501 gcaactgacc attgaaagca cgattcctgc tgaacgtgga atgggatcaa gcgctgcagt
     4561 cgccacagca gtcactcgtg ctttttatga ttacttagca tttcctttgt ctcgtgaaat
     4621 actattagaa aatgtccagc tttcggaaaa aatcgcccac ggtaatccta gtggaatcga
     4681 tgcagccgct actagcagct tgcagccgat ttattttaca aaagggcatc ctttcgacta
     4741 cttttctttg aacatcgatg cttttttgat tgtcgctgat acaggaatca aaggacaaac
     4801 aagagaagcc gtcaaagatg tcgctcacct ctttgaaacc cagcctcatg aaactggaca
     4861 aatgatccaa aaattaggat acttgacgaa gcaagcaaaa caagcaatca ttgaaaattc
     4921 accagaaacg ttggcacaga caatggatga atcacaatca cttctggaaa agctgacaat
     4981 cagcaatgat tttcttaatc tattgatcca aacagcaaaa gataccgggg cattgggtgc
     5041 caaattaact ggcggtggac gcggtggctg tatgattgca ttagcacaaa caaaaacaaa
     5101 agcccaagaa atcagtcaag cacttgaaga tgcgggtgct gctgaaactt ggatacaagg
     5161 attaggagta catacctatg tttaaaggca aagcacgcgc atataccaac attgctctaa
     5221 tcaaatattg gggtaagaaa aatgaagaac tcatccttcc aatgaacaac agcctttcgt
     5281 taacattgga tgcattttat acagagacag aagtcatctt ttctgatagc tatatggtag
     5341 atgaatttta tctagatggc accttgcaag acgaaaaagc aacaaaaaaa gtcagtcagt
     5401 ttcttgacct ttttcgtaaa gaagccggcc tctcactaaa agcttcagta atcagtcaga
     5461 atttcgttcc taccgcagct ggattagcct cctctgccag cgggctagct gctttagcag
     5521 gcgcttgcaa tactgctctt aagcttggat tagatgatct ctctctttca agatttgctc
     5581 gacgcgggtc tggttcagct tgccgaagta ttttcggtgg tttcgtcgaa tgggaaaaag
     5641 gccatgacga cttaagttct tacgctaagc cagtcccttc cgattctttc gaagacgatt
     5701 tagcaatggt tttcgttttg atcaacgacc agaaaaaaga agtgtccagc agaaatggga
     5761 tgcgtcggac agtcgaaaca tccagttttt atcaaggctg gttagattcc gttgaagggg
     5821 atctatatca attgaaacaa gcaatcaaaa caaaagattt ccaacttctc ggagaaacga
     5881 tggaaagaaa cggactaaaa atgcatggaa cgactttagc tgcccagcct ccattcactt
     5941 actggtctcc tgacagctta aaagcgatgg atgcggtgcg acaattgaga aagcaaggca
     6001 ttccatgcta ctttacgatg gatgctggcc ctaatgtcaa agttttagtg gaaaacagcc
     6061 atttatctga ggtacaagaa acatttacca aattatttag caaagaacaa gtaattactg
     6121 cacatgctgg tccaggaatt gcgattattg aataaaggga gatttttatg attgaagtat
     6181 ctgcaccagg caaactctat attgccggag aatatgcagt tgttgaaaca ggccatccag
     6241 cagttatcgc tgcagtcgat caattcgtga cagtcactgt agaatctgca cgaaaagtcg
     6301 gaagtatcca atctgcacaa tatagtggga tgcctgtacg ttggacaaga cgcaacggag
     6361 aattagtttt agatattcgg gaaaatcctt ttcattatat ccttgctgct attcgcttga
     6421 ctgaaaagta tgcacaagaa aaaaacattc ttttatcctt ttatgatctg aaagtaacaa
     6481 gtgagttaga cagttcaaat ggccggaaat atggattggg ttctagcggt gccgtgaccg
     6541 ttgcaacggt caaagccttg aatgtttttt acgcgttgaa tttatctcag ttggagattt
     6601 tcaagattgc agcactagcc aatttagcag ttcaagataa tggttcctgc ggcgacatcg
     6661 ctgctagctg ttatggtggc tggatcgctt tctcaacctt cgaccatcct tggctccaag
     6721 aacaagaaac tcagcattct atcagtgagt tacttgccct ggattggcca ggtctatcca
     6781 ttgagccatt gattgctcct gaagatttac gtttattgat tggttggacg ggtagccctg
     6841 cctctacttc tgatttggtc gatcaagttc accgttcgag agaagataaa atggtggctt
     6901 atcagctttt cttaaaaaat agtacagaat gtgtcaacga aatgatcaaa gggtttaaag
     6961 aaaataatgt aacgttgatc caacagatga ttcgaaaaaa ccgacaatta ctgcatgatt
     7021 tatctgcaat cactggggtc gtcatcgaaa cgcctgcttt gaacaaattg tgtaatttag
     7081 ctgaacagta tgaaggagcc gcaaaatctt ctggtgcagg tgggggcgat tgcggaatcg
     7141 taattgttga ccagaaatct ggcattcttc ctttaatgag tgcatgggaa aaagcagaaa
     7201 tcactccact gccgttacat gtctatagcg atcaaagaaa ggaaaaccga tgaatcgaaa
     7261 agacgaacat gtttcactag ctaaagcctt tcatgataaa caaaaaaacg aatttgactt
     7321 cgtacggatc atccataacc ctttgccgca aatagcagtt tctgatgtcg atctaagtac
     7381 acaggcagtt gggtttacac taagcagccc attttatatc aatgcgatga ctggcggaag
     7441 cgaaaaaaca aaaaaaatca accaagatct tgcaatcgtc gcaagagaag cagatttgat
     7501 gattgccact ggttcagtca gcgctgcctt gaaagtccct tctttagccg atacttatac
     7561 gattatgcga caagaatatc ctcacgggaa aatcattgcc aacatcggtg cagggacttc
     7621 tgtcgaaaga gccaaagaag ctatccgatt gtttcacgca gatgccttac agattcattt
     7681 gaatgcacct caagagttgg ttatgcctga gggagaccgt gattttacca attggaaagt
     7741 acttattcaa gaaactcaaa cagccattga cgtccccctc atcgtaaaag aagtcggttt
     7801 tggcatgaca agagaaacgc tcaatgatct agctgcttta ggagttcata cagttgatat
     7861 cagcggtcga agcggaacaa gcttcaccca gatcgaaaac gcccgtcgct ccaagcggga
     7921 actgagttat ttagctgact gggggcaatc gacagtctct tcattgcttg aagcaaatga
     7981 agcagacact tctatggaaa tcctagcctc cggaggtatc cgcaatgcct atgacatatt
     8041 taaagccctt tgtctcggcg caaatgccgt gggtacttca ggaaccgtac tgacgcatct
     8101 gatgaatcac ggcgtagaag aaactattat actgatgaag caatggcaag aagagcttcg
     8161 tctgctttat actatggtcg gcgcaacgaa taccgcggcc ctacaccaac aatctctgat
     8221 cttttccgga ccagtgaagg attggtgcga agcaagaggg atcgacttag tgaagtatgg
     8281 aagacgcaca gaaaagagcg tgggacaaaa gtaaagaaca cttttgtctc acgctctaaa
     8341 cacgtacaaa cggcggaagt aaaagcaact ccttcggaaa taagctgaaa ttcacaaaaa
     8401 tttgaaaagc aattttcgtg aattccttct tatttcttgg agttaaatgc ttctgtccca
     8461 gcctcttttt tcgaataaat gatgtttaat cagctgtcct tcggtattcg ttcaatccgc
     8521 tgatttcaca acctctccac taaacagtgt actaaagctc ttttaaggtt gttttccgat
     8581 ataggcaagt atgcctccgt ccacatacaa gacatggccg ttaacgaaat cagatgcgct
     8641 agaagccaag aagacagatg gtccagccaa atcgattggc tcgccccagc gtgctgctgg
     8701 cgtacgtcca acgataaatt gatcgaatgg atgacgttca ccgttttctt gtgtttcgcg
     8761 taatggagca gtttgcggtg tagcaatata acctggtcca attccattac attggatatt
     8821 gtattgaccg tattcagaag cgatgttttt cgtcagcatt tttaatccgc cttttgccgc
     8881 agcataagca ctgaccgttt cgcgtcctaa ttcactcatc atggagcaga tattgatgat
     8941 tttaccgccg cctttttcga tcatatccgg aataactgct tttgaaacga tgaatggggc
     9001 atttaaatcc acgtcgatta cttggcggaa gtctgccgca tccatttcga tcatcggtgt
     9061 ccgtttgatg atccctgcat tgttgactag aatatcaact gaacctacat cttctttgat
     9121 ctgttgaacc atcgcttgaa ccgcttcttc atcggtcaca tcgcagacat aaccttttgc
     9181 atcgatgcct gcttctttat aatgcgctat accttcatca actgattctt gattcaagtt
     9241 attaaatacg atttttgctc ctgctgtagc taaagagcgt gcaatctcaa atccaatacc
     9301 atagacagca ccggtgacta atgctacttt tccatctaaa cgaaacatat ccatactaaa
     9361 ttccatcatt ttctctccct tatgcttatt ttaattgatc catttgcacc atatccatgt
     9421 ctgtataggt gatattttct ccgcacattg cccagatgaa tgagtaattg cttgtaccta
     9481 cacctgaatg gatcgaccag cttggcgaaa taatggcttg ttcattgtct acaactaaat
     9541 gttttgtttc atctggtttg cccatcatgt ggaacacacg tgtatcttca ttggcataat
     9601 caaagtagac ataagcttcc atacgacgtt catgtgtatg acaaggcatc gtattccaag
     9661 cgcttcctgg ttctaaaatc gtataaccca tttgtaattg acagctttcg catacatttg
     9721 gatggatgta ttggtagatt ttacgttcat tcagtgtcag gccttcacca gtctccattg
     9781 gttttacttg gtcgatactg atttttacat ttggatattt atgatgagct ggaacagaac
     9841 taatatagaa tttagctggg ttttcagcat cttgagaaga aaatacaaca tgtcgtgttt
     9901 cttttccgat atagtatccg tcttgttttt tcatttcttc tttttgccca tcgatctcga
     9961 tgaatcctgg tccaccgata ttgatcacac ctagttcacg acgctctaag aagtaatcaa
    10021 cacctagttc tttgtctaat gtgatttcta attctttttc agtaggtgtc actccaccaa
    10081 agatcattcg atcgttgtgc gtataagtca aactgatttt ccctggttca aatacctttt
    10141 ctactaaaaa ttcttttctc aattgttccg ttgaataatg acggatatct tctggactgt
    10201 gtgtataacg tgtttccatt tcttgcattt tcttctactc cttctatcga atgatttttc
    10261 cagagccgca tgctaaaaat tgtgcgactt cttcttgact aaattggttg cagtccccat
    10321 ggatcgtatg cttcaaggca gaagctgccg tagcaaaatc aatgatttct tgcggagaca
    10381 tattggataa aatcccatgg agcacgccac ctgcaaaagc atctccgcca cctactcgat
    10441 ccacgattgg ggtgattcta tggacttggg attccacata ttcatggttc atccacaatg
    10501 tccccttcaa ttcattttca cttgctgaat gaacggtacg cttggtcgaa taaaacacct
    10561 gaatattcgg gaacttttcc tgcatttttt gataataata aattagaggt tgttcttctg
    10621 cttctggcgc tttctcgata cctaacaaat aaagagcatc catttttcca gctgagcaga
    10681 tatctactaa aggtaaaagc tgatggatta cttcgctggc ttcttgctgt gtccataatt
    10741 ttccacgata gttgatatcg aagctgatca ggcagccagc tttttttgca gcttctatca
    10801 cctgtttcgt cattttttgc cattttttag acaaagctgg ggtgattcct gaaacatgaa
    10861 atatatctat tccttggaat aactcctcta gcgaccattc caaatgatcc atttcagcaa
    10921 aacttgatcc ggcacgatca tagatcactg aggcggcgcg ttcgccacta ccagtttcca
    10981 tataataagt acctagtctt gatccaccag ataatacctg tttcgtatct actccataac
    11041 attgcaagtg ctttttgact gcttttccca atgcgttatc aggtactttt gtagcaaaaa
    11101 tcgcttcatg tccgtaatta gctaaagaaa ttgctacgtt tgcttcccct ccgccataat
    11161 gggcagcgaa tcgttcactt gaatcgatac gggttccctg ctctgtggac aatcgcaaca
    11221 tgatttctcc gagtgtcact acttttccca taagcagcta cctcttgatt tctttcattt
    11281 ttgctgcata ctgttgcgcg acttccgtta ctttgtcaaa atctcctgta gctgcgggtg
    11341 ctaaaaggtt cccacctact ccgactgtta cgacaccagc atcgaaccac tctttcatat
    11401 tgtccagact cacaccgcca gttggcatga tattgacttg aggcatcggc gccttgaagg
    11461 cagaaatgat acttggtcca taagcgcttc caggaaatag tttgacgata tctaccccgc
    11521 ttttcagcgc tgttttgatc tcggtaatag tcatacatcc tggtaagtaa ggaatttgat
    11581 acagattgca catctctgcc gtttcttgat caaaggaagg gctaacaata tactctgcgc
    11641 cagctaagat agccaagcga gctgtgacag cgtctaatac tgtgcctgcg ccaatcacta
    11701 tttctggttg gtctttgtag aatgacaaaa gttctttgat tacttgatct gcttgaggga
    11761 ctgtaaaagt caactcaata ccagttagac cacctttgat gattgcgtgg caagcattca
    11821 aagcttcttc tttgcttttt ccgcgtacca ccgcaatcac tccggcgttt ttcaatcgag
    11881 ataaaatatc aatttttttc atcttctcca cctatttcct atttagaaat tttattttat
    11941 aacttggaaa tctataccca tattatacgg cagaaaagaa aaattgcaaa cgcttttttc
    12001 attatttttg aattctttgc tcgatttcgc tttttgtttg cttgagggca ctgattattt
    12061 gttcttttgt ctcatctgtt aaacgatatt tcggcatact tacactaaat gcaccaatga
    12121 tttgttcgtt tttcacgatt gaggctccta tacaaaaaat atcacgttcg atttcttcat
    12181 cgtcaaatgc gatgtgggtt tcttttaccg ttttgatttc ctttttcaac cgtaatgcat
    12241 tggtgatcgt atattctgtg taagggatca acggatgctt ttcaagataa tcagtgtact
    12301 gctcttcact gaattctgct aatacggctt tgcccatcgc agaattgtat aatggtcggg
    12361 tgattccgat ttttgaagac atgcggatcg tttgatgttt tggatctagt ttgttcacgt
    12421 acaagatctc attgttgtcc aataccccta agtggatagt ctcatcgatc ttttgctgca
    12481 gctgttctaa aaacggtaaa gtcaactcta ccaaatcgat ttgttcaatg tttttattcg
    12541 cgtagcgaat aagctttgca cctaaacgat agtttttctc tgaattttta tttacgtagc
    12601 cgatcatcaa caaagtatct agtattttca agactgtaga agaggtcatt cccacaccct
    12661 ttgcgatcgt ctgcaaagag acatcttggt tttccgctaa atagtctagt attttagagg
    12721 ctttgatcaa tacagttcca taaggctttt tattttccat ataatattct cctatttctt
    12781 tataagaaaa attatagcgg aaataccatc aatttaaaag agatatccag atataaacta
    12841 ccatttctct ttacaggcaa tttaatgttg aagatccgct tcgtttttac atcaaacttt
    12901 gatataaagc aatgatttct tctactgtcg tttctcttgg atttcctggt gtgcatacat
    12961 ctcgcaaagc atcttctgca attgctggaa tatctgcttc ttttacacct aattcagaaa
    13021 tggtttctgg gattccaacc gctttgctca accgatcgat ctcaccacaa gccgcttctc
    13081 ttacatcttc taaagacatt tcagctgctc ctttaatccc tagaacaaga gcgatctcac
    13141 ggtacttttc tccagtatat tctttgttat atttcatgac agttggcaaa agtgctgcac
    13201 acgctacgcc atgaggaatg ttgtaccatg cacttaacgg gtgagccatc ccgtgtacca
    13261 gtcctaaacc gacatttgag aagcccattc ccgcaatata ctgtcctaat gccatttttt
    13321 ctcgcccatt ttgatcgcca tctacagaag cttccaaaga atggccgatg atttcgattg
    13381 cttttaaatg cagcatatca gtcatttccc aagcgccttt tgtgataaac ccttcaatag
    13441 catgcgtcat agcatccata cctgttgatg cagccaattt tttcggcatt cccatcatca
    13501 tatcgctgtc gatgaacgcg acgattggga tatcatgagg atcaacacaa acaaattttc
    13561 ggtgatttac ttcatctgtg atgacgtagt tgatcgttac ttctgctgct gttccagaag
    13621 tcgttggtat agctaatata ggtaaacatg gatttttcgt atcagcaaca ccttctaaac
    13681 taatgacgtc tgaaaactct ggattcgtca aaataatccc aatggctttt gccgtatcga
    13741 ttggcgaacc tccaccgatt gcgatcaaac agtctgcctc tgcctcttta acaaaagcca
    13801 ctccatcttt tacattttgg atcgttggat taggtacgat cccgtcgtat aaagcataag
    13861 cgattgctgc tttatctaga agttctgtta cttttgttgc tataccgtgc tcgatcaatc
    13921 ctttgtcagt aatgacagct gctttgccga atcctcgctg ctgaaattca gggacgatcg
    13981 actcaatcgc tccttttcca aaatacgatg tttcatttaa aatcattcga ttactcattt
    14041 tctattcctc ctaatttgta ttatttttca caataaaaac gaaagaaccc caagcaaccg
    14101 gttgcccagt tgcttgttgc tacagttcaa agactggcat caattcccaa ctgactggag
    14161 aggcatctgg atttgtctcc atgatatctt ccatgtactt ccaccatttt tgattgatag
    14221 atgtatttgc cattttttcc cataactttt catcttcgat cactaagtaa ccaaataaca
    14281 aacttgtact cggatcaaga tagatcgaat agtcgatgac cccgtgttca cgaagtgctg
    14341 tacgcatttc tggccaaagt tcatcatgtc tttttttgta ttcttcttgt ttttctgggt
    14401 aaactttcat gcaaaaagct tttttcacca tgtcagacac ctgctttcgt atgaacttcc
    14461 tccaaataac ctgcttttgg agtgacacca aacgcatctg ctaaatgcca caggttttcg
    14521 tccgtgattg tttgcaagat tggtccttgc gctttgacgt acgtatagac ttctgctgcc
    14581 ttttctgctg tctcgatcaa accaaagact tcatccatgt cttttcctga cccgtagatt
    14641 ccatgttgtg gccataaaac caatcgagtt tctttcattt tttcagctgt tgcttcccca
    14701 atctcatttg ttcccggaac catccacgga atgatggaaa ttccttctgg aaaaacaact
    14761 aagcattccg tacacatttg ccatagcgta cgactaaagg aacgttcgtc taattcatgc
    14821 gtaaaactca tcgctaatag atgactagca tggcaatgca taacgatccg attatctggg
    14881 tctacttcta aacgggcgat atgactcatg aaatgacttg gcaactcgct tgtaggaagg
    14941 cattcgtttt ccagccccca caaaagttcc agtgttttgc catcttcagc aactcgaacg
    15001 attccaagat tctcggcagg ttcgctcgct acatttttaa aatatcttcc tgaaccagtt
    15061 acaataaaat agcatcctgc taaacggctg gcatcaaaaa tcatcgagat gcggcggatc
    15121 actttttcag gatccaagaa cggtgtgatc tcttcttctt ttagtaaata agaaatattc
    15181 ccgccatttc tttcatccca tcccaagcga taaagattcg acgtcgtttc gatcatttct
    15241 ttcacataag gcgcttgtaa aatatctatt tttttcaata tagccacact cctattttct
    15301 taattccaac acgtcttttt gatattcctg gacttcttcc agccaatcca aaccaactgg
    15361 aacttgattt ttgtaacaga aataattcca tacatcggca tacgggaaat ctttcaattc
    15421 ttccgtataa gccattcttg aagtaaagtc aaaagccagc tccatttctt tcaatcgctc
    15481 aactggttcc agcatagctt tcagcagtgc tttttgtgta tttctcgtac cgatgaccca
    15541 agcagcgata cggttgatcg ttgcatcaaa gaaatccaga ccgatatttg ttttttccaa
    15601 caattcgtta cggactaatt ctttagcgat ttcctgcaat tcatcatcca tcaaaaccac
    15661 atggtctgaa tcccaacgca ctggacggga aacatgcaac atgattcctt tgctgaataa
    15721 agctaatgaa gataatttat tggaaatcac ttctgttgga tgaaaatgtc cagcatctag
    15781 acaaacaagt ttgtcacgag tcaacccgta acccatataa aattcatggg aaccgactgt
    15841 ataagcttca gcgcctaaac caaatagttt gctttctact gcatcttgta cataccgttc
    15901 atctaactct tctgtaaaaa tttcatcgag tgattccatt aaacgttccc gtggggtaaa
    15961 acgatcaatc ggattatctt tcatcccatc aggtacccaa aagttattga tacttgtttg
    16021 cccagtttct ttcccaaagt actccgcgat tttgcggctt cttttcccgt gttcgatcca
    16081 gtattcacga acttctttat ctggatgagc caatgtaaag ccgtctttca tcattggatg
    16141 cgaaaagaaa gtcggattga agtcaagacc gatttcttct ttttttgccc actctaccca
    16201 tttttcaaaa tgtttcggct caatttcatt tagatcgaca gattcttctg tatccaaata
    16261 gatagcatga aggttcaatt tgtgtttgcc cggtatcaac gaataagcct tctccagatc
    16321 ttgtctaagc tcttcaggtg ttcgtgcggc tcctggaaaa ttccctgtcg ccatgattcc
    16381 accggtcaat tcgccatccg gtgtcaagaa acctttgaca tcatctcctt gccagcaatg
    16441 catagaaatt ttgatgtcct gtatcttttc tagtacagca tctgtatcta caccgatagt
    16501 tgcatatttt tcttttgctt gttcgtaatt tctttcaatc gtgttcatac gattactcct
    16561 ttgattttat tcacgtacca ttccatcgtg ttttgcttat tttcatctgg gtaaaaagtt
    16621 tcacatggaa acgactgttt gattacattt cttgcttgtt ctatattcga aaactgacct
    16681 gtactgagca tttgcatggc aacattcccg attgctgtgg cttcactagg tcctgcttct
    16741 acaatgattc cggcataatc tgctgtcaat tgattcagta gacgattatt cgatccgccc
    16801 cctacgatgt gcagcttttc caatttcgtt ccagttaagt gttctacttt gatcagctct
    16861 tgtgcgtaac aaagagcaag attatcatac acacaacgag cgatagctcc cgatgagaca
    16921 gggactggct gctctgtttc aaaacagtat tgctgaatct cttctatcat atttggggga
    16981 ttcaaaaatc gagggtcatt gatttccatc acttgctgca gcggtttttc ttttgcagca
    17041 agctctgcca attccgcata actgtactcg tactcttgca ttcgggcaac ttcctggatc
    17101 aaccacatcc ccatgatgtt tttcaaaaaa cgcaccgtgt tatgggcacc ccattcattc
    17161 gtataatttt cagaaaaagc attgcgagaa acattggcga tctttgtttc aatccctaaa
    17221 agcgaccatg tcccactgct gatataaccc caacgttctc cttgacctgg cgttcctaat
    17281 acagctgacg ccgtatcatg acttgctact gtgataaagg tcacatctgg caaatcatat
    17341 tttgaaaatt gttctttttg cagatctcct agtatcgttc ccggctctgt caatggtgga
    17401 aataattcct tcgagacacc gattttttca agaagttcta tttcccattc ttttgtccct
    17461 gcattcagca gctgcatagt agatgcatta gttttctccg tcaccatgtt accagtaaag
    17521 acatacccta aataatcagg tatcagtaac aatttatgtg cttttgccaa attttctttt
    17581 tcttcagcaa acaattggaa caaggtattg aaaggctgga tctgaatccc tgtctgctca
    17641 tacaacttat ctagagaata gatttcagaa aactgttcta ctgctccatt ggtacgcttg
    17701 tcccgataag caatcggatc atttatccga tccccttgtt catcaatcag acagtaatcg
    17761 actgcccatg tatcgatccc cgcaaaaagg cgatcaatcc ctgtctgctt taatttttca
    17821 aatccaatca aaatctcttt gataagataa tcgatcttcc agcgatcgtg accgtctttg
    17881 ttccgaaagc cgtttttgaa ccggtgtact tcttccaaga tcaaagaacc ttttgcatcc
    17941 ctcgtacttt tcattagtcg tccgcttgat gcaccgatat cgatggcaac aaatgttgga
    18001 ttcatttctc cacctctttt actttattgt tgattagcac tttattaata aagcgttatc
    18061 atttattgac aatgctatca taatacagaa tcttatattt tgcttcacac tattttggta
    18121 tataattgca ttatcttgac aagttgggag tgtttacatg gattactggg aaaaagccaa
    18181 ccactcatgg tcggaagact caaaaagagt gatcttgacg cctacacaaa gaagcaggaa
    18241 tttatttttt tatatccaag aaatcggtta cttcaaagca ccaatccctt actatactga
    18301 acgagcacat ttaccttctt acttgataaa gtttactttg tctggcaaag gttcgcttac
    18361 atataatgat aatcagcaac agctaaaagc tggagactta ttttttattg actgccaaga
    18421 atatcagcgt tatcaaacga tcagcgaaga accatgggaa atggattgga tccatttgaa
    18481 tggtcagaac gtccaatcat tttatcagga gttcacaaaa gacggccata acaccttcca
    18541 ttcaacagga gatccatcag aaaacaagat ccaccatttg atcacagaat tgatccacat
    18601 gcaacaaggc acgaatgccc gtaccgattt ccagtcctct gtagctatcc acgagttgct
    18661 gaatgaactg attttgcaaa agtatcagct agatttcgaa gaagaggata ttcccgccta
    18721 cgtttacaaa ctgaagcaat acttagacga acatttcaaa gaaacgatta cacttgatca
    18781 attagaacaa caattcttat tgaataaata tcagctgagc aaagattttt ccaaatttat
    18841 cggttctcca ccaatcgatt atttgatcag caaaaaactt tcttacgcta aagaccttct
    18901 tcggtataca gaatttacgg ttcgaatgat ttccgaagaa atagggattg agaattttgc
    18961 ttactttagc cgcctgttca aagcaaaaac aggactatcg ccgagagact ttcgtaaatt
    19021 cagctgattc atataatatg tacgtcattt tttcaaaata ttttgaaata tatagataaa
    19081 aataaaataa ccgcagtttc tgcttctatg taaacaacag cagctactgc ggttattttt
    19141 tagtgatcat tctcattgtt catcttcaaa atgttcataa tgaaccaact gtttctgtgt
    19201 atcacgtttc aaaaattctt taggcgttat gccgaactct ctcttgaatg ctttcagaaa
    19261 atgcggataa gaagaaaaac cacttttact atatacagca ctagagctgc ggtattctcg
    19321 caacaagtgc ttggcataga tcagcttttt cttcattaca tactgatgaa acgtaaatcc
    19381 agtgtacttt ttaaattctc gtgtgatatg gtatttgctg acaaaaaact gcttttccat
    19441 ttcctctaaa gaaagcggat gagataaatt ttttgagata tattgtaaca tttgttccac
    19501 acgctcatta ggaactacag cattctgttc ttgcgcactc tcttcttttt gtacaacttg
    19561 attgagaaaa atcaagtatt ctaccagagc ttgttcatat tttatgtctt caccgtaaat
    19621 ttttttatca ggaggatggt cgataaatga taatttttgc ttcaatactt cctgttctat
    19681 ttgcaataca cgactcctct tctctcccat tggttgaaaa caagccgata gattagacca
    19741 tggcgtggaa cgactttcta gaaaagaagg ggtgacaaag atatagacac gctcgaatac
    19801 atctgaagat tggcggacga tacgatgcaa atcattggaa tggatgagca gcacacttcc
    19861 tgctttgatc gtaaattgtc gtccgttcaa ataaaaagtc gcttctccct ttaatgtagc
    19921 atggatctca tagaaatcat gatgatgata gatcgtttta ttttggctga cgacatcttt
    19981 gacatgatag atttcatact gatcagaaac catcgtattg acttgttcac cgctgatttt
    20041 cttttggagc atgtgccgca cctccttttg ttcacttatg cacaatttta ttataccacg
    20101 ttttttgtaa tcaaaaagag ctgcttttat atactcttat aaaaaagaag agacgtggca
    20161 tcttctagcc ccgccccctc cattctaaat attagttaac caacgtcttc tttacattat
    20221 tcaagaagac ttcttccata acgaatccca tcatcttttg tttcttcaag aaccacatac
    20281 aggtacggac gattcttcat gatgatttct ttgatttttg tgtactcgat cacaccgtct
    20341 cccaaattta ccggcacgat tttttcctgt tcgacacgaa aatctttcaa atgaaccgca
    20401 catatctttt caccaaaacg ttcaaaagct tcttctacca attggacttg ttcttgataa
    20461 tcactacttg tgatcaaatt ggtcggatct aaaatgattc ctagatagtc cgaatctact
    20521 gcttggatca gctgctctac ccttgctaaa gaatacaccg gatgattcaa acctggttct
    20581 atccccacca tcatttggta tttttctcca gcttttacca agcgacggat aacggacaca
    20641 gcttcagcaa atgcttcatc tgtgaaattt tcttccgtat attggatttc cggcaaaaca
    20701 catccagttt ccgaagcaac catcgacgca ccaaaggaag aagcatagcg aagatactgc
    20761 tcgaactttg tcagcaactc ttgtcgtatc gttaaatctg gatgaatcat attgatatag
    20821 caacttaaaa ttcctacggt cacttcttgc ttttctaaaa ctcgtttgac ataatttccc
    20881 atcccagaat tgatctcatt tgcgccagaa ggtatttcag gaaacgaaag acctaaggcg
    20941 agctgcagtg tatgaatccc ttgttctttt gttttttctg caagatcttc tatcgtttgg
    21001 acttcggaaa gatcatgtcc tctaattgta atgttcaaca taactattct cctaactcag
    21061 catattgggt agccacatga cgatttgcgg gaagtaagta atcaaaaaca agacgactaa
    21121 gataacccca taaaaaggaa gaagcggttt cagcattgcc tctgctttta cgcctcctac
    21181 acttgcaccg acatatagtc ctgtacctac tggcggcgtg atggagccta cacacagatt
    21241 catgattgaa aacaacccgt aatgtacagg atcaacccct acacttgtga cgattggtaa
    21301 aaatattggt gtaaaaatca agatagcagg cgccacatcc ataaacatac ccaccagtaa
    21361 taacacaaga ttgactaata acagaataat aaatttatta tccgttacgc ccataatcaa
    21421 cgaactaatt gcctgaggaa taccggtaaa cgccatggcg aaagacatca tggaagaagt
    21481 agccaataag aacatgatcg tccctgacat tgctactgat tgaaacaaca tattaggtat
    21541 atcttttagt tttaccgttt tatagtaaaa catagcaatc aacagggagt aaatgacagc
    21601 tatcgctgaa gcttctactg ctgtaaatat ccccgtcaag ataccaccga taatgatgac
    21661 aatcaataga aggctaggaa tcgcctcttt tattattttt ccagcatgag ccatctgtcc
    21721 tttttcgaca acagggtatt tattcttttt agcaacgatg aatgctacta gcatgattcc
    21781 tagcgcccac aaagcgccta cgagataacc tcccataaac aaagcagaga tcgaagcacc
    21841 accagcaaca agtgaataca tgataaaagc agtacttgga ggaatgacca tacctgtcgg
    21901 agctgacgcg atattgaccg ctgtcgcaaa cttccggtca tatccttctt ccacttgctg
    21961 agggatcaac actccgccaa tcgcggtaga agcggcaatg gccgagctag agatggaacc
    22021 aaataaagca ttccctaaca cattcgtatg agccaaagcc cccggcacgc gcccgacgaa
    22081 caacatcgca aaatccacta gtttcttagc tatccctcca ttattcatga taatgcccga
    22141 gagaacgaaa aacggaatag cgatcagcga aaaactattg atacttccca ccattttttg
    22201 agctgaacta aaagcagaaa catcaaacgg tataactaac aataaagtca tcatcgatga
    22261 gaatgcaata cttatagcaa tcggcatccc taatacaagc aacacaacaa atacaaaaaa
    22321 taacactaat ccggcttcta ttgccatttt attcctccat ttttatattt tttcttgaat
    22381 tgctggcttg ttgcttaatg tatcgattag attgatcagg ctatagataa tgatgagtac
    22441 ccccgataca ggtactgaca gataaacata tcccatagga atacttaaag aaggcgagat
    22501 ctgccccatt gtcaatgtga cagcattcca tccgccatac accatgatca tcgcagcaaa
    22561 taccagaaat aaactttgaa caacaacttc taaaatttta ctttttttgc cagttaattt
    22621 ttcacttaaa accgttacgg ctaaatgtgc tttttgtccc acaacatacg cggaagacaa
    22681 catggcaaac cagacaagac cgaatcgaac aagctcttcc gtgaccgtat ttggatttcc
    22741 taaaaccgtt ctggaaaata cttgccataa aacaacagcg accatcccaa caagcaaaac
    22801 agctcccagg atctctaata ccttgtttaa aatcattttc atcacagcta cgctccttgc
    22861 tcttggatct tttgataaag gtctttgtag gtaggatcgt ttttgaatat atcatgaagt
    22921 ggggcaactg cctctcgaaa cggtgtatta tctacatcat tgaattggac tccatgttct
    22981 tcttgtgcta aagctttttc ttcttcaatt gcttttgcaa aagcttcaat ttcaaaaacc
    23041 gttgattctt tcgctgcttc ttcaatggct ttttcctgct catcagttag ctgttctttg
    23101 atggcatcat tcatgatgat gatatctggc acacgtgtat gctcatcata ggaatagtat
    23161 ttagccacac caccatgtcc agctgtatac aagacaaatt cattgttttc tgatccgttg
    23221 atcaaattgg attgaagaga cgtgtacact tcatcacttc ccataggtac gggcgaaccg
    23281 cctaaaagct ctaccatttt gatagcggtt tcgctttgca tcacacggat cttctttccc
    23341 ttcaaatctt ccggcgtatg gatcggaccg tctaccatat aaaagctgcg ctgaccagaa
    23401 tcataatagg tcaatccggt aaatcctaaa tttttggtcg attggtagat tggctccatt
    23461 atttcttcat tatccatcac cttaaagaaa tgttcttcat cattgaatag ataaggaact
    23521 gaaaaaatcg aataatcttt agagaaactt tccaacgctg aggcacttac ttttgtgaaa
    23581 tctactgctc ctgactgtgt caattcgatc agttcacgtt ctccacccag ttgtccatct
    23641 gggaaatact ggatctgtac ggaaccatct gttttttctt ttactaaacg cccaaattca
    23701 ttcattgcat caattactgg ctggctgttg cttgcataag caaagcgcaa ggtaactatc
    23761 tcatttacat ctgcatctgc gacagttgcc acactagaat tatttccgca gccgcttaga
    23821 cctagcagac ctaggctaag cgaacttgct gcaaacagca gtgatgtttt tttcatatgg
    23881 gtttcctttc ttttaacaat aagagacgag caattctgtc aaacaaagca ccgttaacga
    23941 ctgtccataa ggcattgctg tgattccgat attccgataa aaatctaaac tgtcccccat
    24001 tcctgtcccg attgaaacgt tttgaacttc tcctttttca tcgatttgtt caagcaatcc
    24061 ttggatagca cgataagcga cttctttata ttcttgtggt aaataacgtt tatggaccgc
    24121 tttcaaaata ccgtaagcaa atcctgcagt tgcagaagat tctacgtaag aagaaggatc
    24181 atccaacaac gtatgccata aaccgctttc agactgataa gtttttaatg cacgcacttg
    24241 tgcttcaagt gtttcgatca ataatttgcg caatgcatct tctttcgata attctaagat
    24301 ttcaatgatc tctggaatag caatcgtcaa ccagcagttt ccacgagccc aaaatgcttc
    24361 ggcatagtta tgatttcctt caaacgtcca gccgtgatac cacaatcctg ttcgtttatc
    24421 catcaagtat ttgatatgga tcataaactg gtaacgagct tcttctaaat attctgggcg
    24481 attcaacagt ttcccgattt ttgccaaagg aagcactgtc atcatcaatg tatcgtccca
    24541 caactgattc ttattttccg gtccatatgt ggcatgctgt aacccgtctt catttgtccg
    24601 aggcatgtca ttcatgaccc attctgccca ttgttctaaa taaggcaaat agcgtgaatc
    24661 tttcgtgtct tcatacaagt aagccatagt caatagcggt gccattgtat tgacgttttt
    24721 gggcggagcg ccttcttcca tacgatcctc aaaccattca ttgaccactt gataagcgcg
    24781 aggatttttt gtcatctggt aatttttata aataccatat agaccgacac cttgtggcca
    24841 attccacact gtccagcttt tgtcatctac tttcagtcca tcaaagtcca acaaaaattc
    24901 accattatcg tctttgatcg ttgtcaggtt ttcaacgagc aggtcaattt tttctttcac
    24961 taattcttta ggtacaaaaa tctcttcttt aataatcatc tttacgccac ctttttaaac
    25021 gctttcattt tatatcctta atatagcagt aataccgtcc actataaata tcgctttttg
    25081 aacaaaaact gtctaaaatt gacctaaatg ttaaataaca aaagtaactt tcagttactt
    25141 ggaaattaaa aaaagaactt ggagaaacaa cgttttccaa gttctttttc atctattttc
    25201 ttatcttttt tctacgcttt cttttgctta tccgttgtaa gcatcaatga tgatctgttt
    25261 caattcagag atcaatggtt ctttcggatt agctgtcgta cattggtctt cgtaagctaa
    25321 ttcagccata cggtctacgg ttgtatctag tgtttcttgt gtgactcctt gagcttttag
    25381 actcatgttg atgccgacag attttcctaa ttcataaaca gccgttgcta aagcttcgac
    25441 taattcctct gttgtttcgc ctttcagacc taagaatttt gcgatatctg cgtaatctgt
    25501 atctgcacgg aaatagtcat atttagggaa catcgcatgt ttttgtggat cttttgcatt
    25561 atagcggata atatgcggta aaagaatcgc atttgtccgt ccatgaggga tcccgtattc
    25621 cccgccgatt ttatgtgcga ctgagtgaca aatacctagg aatgcgttag caaacgccat
    25681 accagccatt gtagaagcgt tgtgcatttt ttcacgtgat tccatatcag gtgtttttac
    25741 tgatttttct aaatagtcaa agactaactt gattgcttgt aggctcaatc cacgtgtata
    25801 atctgaagcc atgactgaaa cataggattc gattgcatgt gttaacacat ccatcccggt
    25861 atctgctgtg acagatgctg gaactgacat gacgaattga ggatcgacga ttgcgacatc
    25921 tggcgtcaat gcataatctg ctaacggata tttcacatgt gtttcactgt cagtgatgac
    25981 cgcaaatggc gttacttcag aaccagtccc tgatgttgtc gggatacata cgaattgtgt
    26041 tttttctggt ttgtcgatct tatatgtccg tttacggata tccaagaatt tttgttttgc
    26101 accaaagaaa gatgtgtctg ggtgttcata gaacatccac attcccttag ctgcgtccat
    26161 tgcagaaccg cctccaagag cgattactgt atctggcttg aagtctgcca tcagtttcgt
    26221 tcctgcgtat acggtatttg tcgatgggtt cggttcaaca tctgagaaga cttcgatctt
    26281 cacatcattt ttacgtcgtg ccaatacttc acgaactgta tcgcagtaac cgaattggac
    26341 cataccaggg tcacaaacga tcatgacacg ttcaacgttt tccattttct ctaagtacaa
    26401 tagagagttc ttttcaaaga agatttttgg cggtaatttg aaccattgca tgttatttct
    26461 ccgtttcgct acagttttga cattgattaa gtttacagca gagacgttac gagaaacaga
    26521 gttcttacca taagaaccgc atcccaaagt caatgacgga atcatttcgt tatagatatc
    26581 accgatgccg ccttctgcag atggtgtatt cactaaaata cgacaagctt tcattcgaag
    26641 accgaatttg acatgcaact cttcgttttc tgaatggata actgctgtat gtcctaatcc
    26701 tccaagattc aacatacctt cacacaaatc aaacgcatgg tccgtgttgt tagctttgat
    26761 catggcaaga actggagaaa gtttttctct tgatagcgga taatctgggc ctacgccttc
    26821 caattcagca atcagcattt tcgttccttt tggtacactg attcctgcca attttgcgat
    26881 ttccatcgca gaatgtccaa cgattgatgg attgaccgca tattttcctt cgttcatcac
    26941 tgcatcttct aatttttgca attcatctgg ttttacgata taaacttggt gtgcttggaa
    27001 ttctgctttt actgctgcat agatttcttt atccacgatc acagcttgtt cagaagcaca
    27061 aatcattcca ttatcaaatg ttttcgaaac gatcaaatcg ttcactgcac gtttaatttt
    27121 tgctgttttt tcaatataag ctggtacgtt acctggacct acacctaaag ctggttttcc
    27181 tgtagagtaa gcagacttga ccattccagc tccacctgtc gctaagacga tcgcgatacc
    27241 tggatggttc attagcatag aagtcgcttc gatcgaagga tgttcgatcc attggataca
    27301 attttcagga gctccggcag cgatagcagc atcacgtacc actcgtgctg cttctgcaga
    27361 agatttttgg gcacttggat ggaaagcaaa aacgattgga tttcttgttt tcaacgcgat
    27421 cattgatttg aaaatcgttg tggaagtcgg attcgttgtt ggagttactc cgcaaaccac
    27481 accgactggt tcagcaattt caatcaaccc tttttggaca tcttcattga tcacaccaac
    27541 tgttttatca tgtttgatat tatgccaaat atattcggac gcatacatat ttttgattgc
    27601 tttatcttcg taaacaccgc ggcctgtttc ctctacagcc atcttcgcta atggcatatg
    27661 ttgatccaaa gctgccattg ccatttcatg cacgatatgg tctacttttt cttgatcaaa
    27721 gctttccatt tctttcaatg ctgcatttgc tttttctgcc aaatcatcaa tcattgcttg
    27781 tacgtcaatt gtcttcgttt tttctttttc tattacttta gccatcatta tcctcctaat
    27841 gggtcatggt tatttgttaa ataattcaca tgtatatcat acaatattta tttttctatg
    27901 taaacacttt tttgtgattt ttttcacagt tatttttaaa tataaaattg ctttatttac
    27961 aggtgtttgt atagaaaaat atataaagtt atttattatt atttatatga cacacccgat
    28021 tattcaatat tgagcaatat atcccatgct cttatctctt tcttttttga ataaaaaatg
    28081 aggtaggaca tctttctgcc ctacctcgtc tttaaataag ctttgatact tatttttctt
    28141 ccttctcttc tgtctcttcc agcgtttctg ttgtttcagt ttttacttca actgtctcag
    28201 ctggttcttc tgttgtttca acagtagtcg ttgcatcatt tgttacgaca gtccctggtt
    28261 tgaccgtttt gattgctgca cgatcgtact caaggaagat cccttcgcag tctaaaacaa
    28321 ctgtccgttt gtcattgtcg atttctgaaa caactccatg taatccgccg atcgttacga
    28381 ctgaatcacc tggtttcatg ctatctaaca agttttgacg ttcttgctgt tgtttctttt
    28441 gcgaacgtga cataaaaaac cacattgcta atagcgcgat aaacatcaca agcattggta
    28501 atgttcccat ttttttcacc tcactaattc tgtatctaca aaaaacactt tagcagaaaa
    28561 atgctgataa cactagtttt ttacttaatt tttaaaagat tttcctttag aagctttttg
    28621 cgttttcttt attgaacccg tattcttcaa aaaaggcttc tctaaattca agcaaattat
    28681 catccatgat tgcttgacgt acttgtttca ttagattcaa caagaagtat aaattgtggt
    28741 aagaagtcaa acgaatccca aatgtttcat cacatttgat caaatggcgg atataggcac
    28801 gtgtatagtt tttacatgta taacaatcac atttctcatc aattggacgg aaatcatggg
    28861 catatttcgc atttttcact actagacggc cttgagaagt catacatgtg ccatttcgag
    28921 cgattcgagt aggcaagaca cagtcaaaca tgtctactcc acggataaca ccatcgatca
    28981 atgaatcagc agcaccgaca cccatcaagt aacgtggttt gttttcgggg atcaacggtg
    29041 tagtgaattc taatacacga ttcatttctg atttaggttc cccgacagat aacccgccga
    29101 ttgagtaacc agggaaatcc atcccgacta gatctttcgc gctttgacga cgaagatctt
    29161 caaaccctgc tccttggatg attccaaata atccttgacg gtctggatta gcatgagctt
    29221 tcaatcctcg ttctgcccat cgagacgtac gttctactga ttttttcacg tagtcgtaac
    29281 tttcatcgaa tggtggacat tcgtcaaaac tcatcatgat atcagagccc aatttatttt
    29341 ggatgttgat tgctttttct ggtgataaaa acatacgcga accgttcaag tgatttttga
    29401 aatgcacgcc ttcttcttcg atattccgca tatcacttaa tgaaaaaact tgaaagccgc
    29461 ctgaatctgt taaaatcggt tgatcccagt tcataaattt atgcagtccg ccagcttctt
    29521 ctactaagtc ttcaccaggg cgtagccata aatgatatgt attacttaaa ataacgccag
    29581 cacccatttc tttcaactct tctggggaca ttgtttttac tgtagctaat gttcctactg
    29641 gcatgaacat tggtgtgggg aaagttccgt gtggcgtgat caattcccct aaacgcgcgc
    29701 cagtatgttt ttctttcttg attaaacgat agcgaatggc aggttgcgtc attcatttcc
    29761 ctactttcta tataaaatat cttgacaact cccaccatca taatcgtttc aagtgctttt
    29821 tgcaagttcc ctcggcttcc ttctacttta tttaatattc ttttttttat ccttttttta
    29881 tgaataacaa aaaaaagaaa aacattatga cagaaatatt ttttgataca tggtatattg
    29941 gtgttattaa agaaaaggag ggaaaaatca tgcaatcttc tgcagaatta aaacgccaag
    30001 caaaagagag tctaaaagga cgttggggac aagctgtttt actaaatcta atccctacat
    30061 tgattggtat tgctactatt cttcttatta tgattcctat tatcttgttt gccaccacca
    30121 tatatgatgg atcagcgagc gtctcagaag cgacaacttc aggaggcggt agttcaggag
    30181 gcggtggcgg aattatttcc agcatcatta gcgctttgtt catgagcggt atttcttgga
    30241 cttatcttga tattttacgt ggacaaaaag atcagatcga accattcaaa gatgcatttc
    30301 gtggattcaa aggtttgttt atcggcggag tgatcagctt agctgtgctg atctcgatct
    30361 ttacaacatt atggggtttg ctattagtta ttcctggaat cattaaaagc tattcttatt
    30421 ctcaaagcta ttttatttac tacgatatcg tccaagaaac aggacaaaca cctaaagtac
    30481 tggatacgat cactgcaagt cgaaaattga tggatggtta taaaggacaa cttttctggt
    30541 tagatctttc atttatcggc tggcatatct tagcattagc aactgctggt attggttatc
    30601 tatggctcag cccatacatt tctgcaacaa aagcagcatt ttatgaacaa ttgccaaaag
    30661 atatttaaaa gagagtgtga cttgtgaccg agggggtggc aggagaacac cgtttatcga
    30721 gaggcagtaa aagaaacatt cagcccatta gcaaaaagga ggtgtgacaa cattcatgtc
    30781 acacctcctt tttgtatagt tccagtaaaa agaaaatcaa cttagaaaca gttaatcatt
    30841 cctcttcttc tgcttccggt aatcgatgga acttgatcag ttcattcacc gcgattggta
    30901 cgatagataa aatccctacc aagaaccagt gattcaaact gatgtgtact aggccgaata
    30961 gttcttgcgt aaatggcagc aaagcaacag ccgttgtaat agctaatgcc agtaacgcca
    31021 tttctaccaa agctttattg gtcgctaaat tgactttgaa gatcgattgg ctgctgcgta
    31081 cattgaacac atgcagaatc gatgaataag ctaagatcaa gaatgcaaca gtttgaccga
    31141 catgctgact tgcgtctacc catgggctga tagtatcgac ataactacct aaataataac
    31201 caaacaaggt tacgatggtg aagacccctg catttatggc aattttttgc aataaacctc
    31261 gagcaaagat cccttcattt ttcggaatgg gtggctgatc catgatgccg tgttccgctg
    31321 gctctcttcc taaagcaaat cctggaagtc catcagcaac tacattgaca aacagtagtt
    31381 gcacagctgt aaatggtgct ccccatccta acagcatcgc aatcaaaaca ataaagatct
    31441 ccgaaatatt acagcttagc aaaaagttga tagctttacg aatattctga taaaccgaac
    31501 gtccttgact gacagcgtct acgatcgtcg caaagttgtc atccgtcaaa atcatatctg
    31561 cggcactttg cgccacatct gttcctgtga tccccatggc acacccaacg ttacttgctt
    31621 tcaacgctgg tgcatcattc acaccatccc cagtcatcgc tacgactgca cccgtacgtt
    31681 gccaagattt gacgatccgg atcttgtctt ctggcgttac ccgagcatag acagagagag
    31741 ctttgacacg cgcatctaat tcttcgtctg ataattgatg cagttctgat cctgaaagtg
    31801 cttctgacgg atcattcaag atacctaatt ctttcgctat ggcactggca gtcaccacat
    31861 gatctccagt gatcatgact gttttgatgc cagccttttt cgctcgcttg attgctcctt
    31921 tactttctgg acgtggtgga tcgatcattc cgatcagacc aatcaaacgt aagtcttttt
    31981 ccagtgcttc agaagaaata tcttgcggtt cttcgtcata agtagcataa ccaacagcaa
    32041 tcactctcag tgcccgttta ccaaagcgat cattaacgat cgccgcttgt tctacatcgc
    32101 ctgaactaaa gcgcggagca agaacatcga atgctccttt ggtgattgag atgtattttt
    32161 cgcccatttg gtgaactgtt gtcatcattt ttcgttcaga atcaaaagga agctcgttta
    32221 ctcgtggata tttttcttct aattctgcct ttgtatgata attctcttca acagccctta
    32281 cgatggctac ttctgttgga ttacctttga taacaaggtc gtcatcttct tgctcgaccg
    32341 tcacatctgt acatagtgca gccattttca atatttccat cgcgctgttc gtcattgaat
    32401 cttcgatatt cgttacttca tcttctttgc tccatacccg gcggacgcgc atttcatttt
    32461 gtgtcagcgt tcctgttttg tctgagcaga taacattagc ggttcctagc gtttcaactg
    32521 ctggcaactg tcggatgatc gcatgtttcc tcgccatctt ctgcacaccg tatgcaagtg
    32581 tcaacgtaac aataacagtc aaggtttccg gtacagctgc aacagccaag gaaatcgaag
    32641 tcatgaacat ctcaaggagt ggttctcctt gcagttcacc aatcacaaat actaaagccg
    32701 cagccgctaa agcgatcata ctgattcgtt tgcctagctg attcaatcgg acctgtaaag
    32761 gtgttttttg catagtattt tcgttcaaaa gatccgcgat cttacccatt tctgtagcca
    32821 tgccaatggc agtgacgatc gctttcccac gacctgcagc aaccgtacat cctttaaata
    32881 ccatattaag ttggtcacct aatgactcat cttcttcagc aataaatgct gcatcttttt
    32941 caactgcttc actttctcca gtcaacgccg attcttctac acgtaattgt gatgcttcga
    33001 tcaaacgggc atcggcagaa atagcatcac cggactctaa aacaagaata tcacccacta
    33061 ccagttgctc tgactcaaca gtggtcactt tgccgtctcg tatgacagtt gccgtttgtt
    33121 tattcatatc ttgcaaggat tccagtgctt tttctgcgtt tccttcttgg acaatcgcca
    33181 ataccgagtt gatcacaacg atagcgatga tcagcagtcc ttcaaataaa tccccatgtt
    33241 ctgtagcaaa tgcggtataa aaggaaatca ccgctgctac aagaaggatg atcgttgtaa
    33301 aatcggacaa gcttcgaaag aattttttga tcatcgattc tttcggcgcc tcatcgaatt
    33361 tattcaatcc ctgctttttt cgccgttcat ccacttcttg tgaagacaac ccagtatctg
    33421 gatctgcttc tgttaccttc tttacttctt ctattgtctg cttgtattct tccatcctat
    33481 tcctccttta aaatcttcgt acaaaaaaag gtacttattt tactataaca tccttttttt
    33541 atttacttca aataaagggc ttatcgatta tttgttaaga aagcgtaaaa tttaaagtgc
    33601 ttatatttgc aaacgctacc aaaggatgct attatataga tgtaaggaag gaggatacca
    33661 tcaaatagac agaagaattt ctcgatagaa cagtgtaagc aaacaaagtt gttagaagta
    33721 caggtttcac ccagacatgc gtgtagagta caaacctata aatagtgaat gcgtatggat
    33781 gtttggatca cgaagtatcc gaaacaagaa gtaatgcgat acgtaacaat tgaataacgt
    33841 aaacgtctga aaattgaata ggaacaagag atgtttcaaa tgttgaagat gtaacgaaaa
    33901 caaaattttt gtttaacatg aagaaattca acaccgtgct ttgcatgtat gatggaaagt
    33961 tacaatcatt tctgtttatt tgaatccttc cgaggtacaa gaaagccgag cttatcgaga
    34021 gctcggcttt ttctttgtca taaaatcttt gtaaagcttt tttgttattc aaaactagcg
    34081 gcttttcata accttatttc acgaacatcg catctccaaa actaaagaaa cgatagcgct
    34141 cttcaatcgc atgctggtaa gcagataaag tcaattctct tcctgcaaag gcactaacta
    34201 acatgactaa agtcgatttt ggcaaatgga aattagttga gaaagcttct accactttga
    34261 attcatagcc aggcgtaatg aaaatatcag tccatccgct gtctgctttg atttctccat
    34321 taaatttcgt tccaatcgtt tctaatgttc gaattgaagt tgttccgaca gccacgattt
    34381 tcccgccatt tttacgtact tggttcaatc gctcagccgc ttcttccgtc aaacggtaga
    34441 attcactatg catatggtgt tcttcaattt tatctacgct gacaggtcgg aatgtaccta
    34501 accctacatg tagtgtcaga tagactaatt caacaccctt tgcttcgatc tcatcaagta
    34561 attctttcgt aaaatgcaat ccagctgttg gcgctgcagc agaaccattt tctttcgcat
    34621 agacagtttg atagcgatct gggtcttcca gacgttcttt gatatatgga ggcaatggca
    34681 tttctcccaa tgattcaaga atctctagaa aaattccttc atactcaaag tcaatgatcc
    34741 gtccgccgtg ttccaattct tctttcacta ctgcttgcaa tctgccatca ccaaaagaga
    34801 ttcttgtccc aactttggca cgtttcgcag gtttgatcaa tgtctcccac gtatctcctt
    34861 gtgtattcgt tagaagaagg acttctaaat gcgcaccagt atctggtttt tctccatata
    34921 aacgagcagg cagaactcgt gtattattca taactagcgc atcaccaggc tgtaactctt
    34981 caatgatttc atggaaatgt ttatcttcca tctctccagt tttccgatcc aaaacaagta
    35041 aacgagaatg atcgcggtct tttaatggtg tttgtgcgat taattcttcc ggcaagtcaa
    35101 aatcaaaatc ttctgttgtt aacatatcgt cactcacttt ctatcatgtg cttcatcatt
    35161 ctatcatgtt tttcgttctg ttggcaaaat cagcactttt tcacttctat tttttgtttt
    35221 cctaaataaa aaaactaccc tatttgtatt ttttcattgt tttaggcata ctagtaatag
    35281 atagttcttc attgaaaata gaaaaagagg tggcaccatg attgaatttc aaaatgtgtc
    35341 aaaaatttat aaaggtggaa aagtcgctgt agaggacgtc aatctttcat ttgaaaaggg
    35401 tgagttcatt tgttttatcg gtacgagcgg aagcggaaaa acgacgacga tgcggatgat
    35461 caaccgaatg actgatccaa cacaagggaa aattttgatt gatggaaaat ccatccaaga
    35521 aattgatcca gtggaattgc gacgtcagat cggctatgtt atccaaagta ttggattgat
    35581 gcctcacatg acgatccgtg aaaatatcac tcttgtccaa aaattgttga aagtcagtca
    35641 agaagaacgg aataaaacag ctgaaaaaat gattgatctt gtggaattgc cccgtgagat
    35701 gttagatcgc tacccacatg aattatctgg tggtcagcag cagcgtattg gagtcgttcg
    35761 tgcccttgca gcgaatcaag atatcatctt gatggacgag ccattcggtg cgcttgatcc
    35821 aatcactcgt gattccttgc aagaccttgt caaagattta caagagcgtt tgggaaaaac
    35881 gatcgtattt gtaacccatg acatggatga agcgattaaa ctagcaagcc gtatcgccat
    35941 catgagtgaa ggccgtgtga tccaatttga tacaccagaa aatattttac gtcatcctgc
    36001 aaacgatttc gtagaagagt tgattggaga agatcgccta ctacaagcca agccaaatgc
    36061 gacaagagtc ggcgaagtta tgctaaatac agcaatcacg attacaccgg aaaaatcatt
    36121 acaagaagca atcaaactga tgagggaaaa acgtgtagat acgttgttag ttaccgataa
    36181 ttcgaatgta ttaaaaggat ttatcgatgt agaaacgttg aataaaaaac gaggaaaagt
    36241 atccagtgtc ggcgacattt tgaataaaca tgttttttac gtaaaagaat ccgcctatct
    36301 tcgtgatacc ttgcaacgca ttttaaaacg cgggctgaaa tacgtgccgg tcgttgatga
    36361 acagaacaaa gtcgtaggta tcctgactcg tgcatcatta gtggatatcg tctacgatgt
    36421 gatttgggga gaagatcccg caactactga tgttgcagag tcagctactc ctgaagaatc
    36481 taaggaggtc tgacctatgc agcagttttt tcatcagcac ggttcagaaa ttctctcaaa
    36541 aggttttgaa catctttata tttcaggact tgctttgatc attggtatca tcattgctgt
    36601 accgcttggt gtcttactga cacgttttcc aaaagctgcc acgattgtga ttggcttaac
    36661 cagtgccttg cagacaatcc cttctcttgc tttattggca ttgatgatcc cgctatttgg
    36721 cgtaggtaaa ttacctgcga ttatcgcact ctttatttat tcattattgc cgattttacg
    36781 taatacgtac attgggatga aaaatgtcga ttggaattat cgggatgtgg caaaaggcat
    36841 ggggatgact gagttccaat ccattttttc cgttgaattg cccattgcta tgccaacgat
    36901 catggctggt attcgtttag ctgccgttta cgtgattgca tgggctacac ttgcctctta
    36961 tatcggcgct ggtggtttag gcgatttgat ttttagtgga ctgaataatt accaaccaga
    37021 tctcattttg gcaggtacga tccctgtaac aattttagca ctattggctg attggcttct
    37081 aggtctcttg gaacaccggc tgacaccgat cgcacttaga gaggagaatg aagaatgaaa
    37141 cgtatactaa aaatgaccat ggttcttttc tctcttctta tacttgcgag ttgttccttc
    37201 cctggtcttg ccagcaatac agatgaagac accatttcga tcacaggtgg aatcacttca
    37261 gaagcacaga tcttagcaag tctagttgcc ggaatggttg aacattatac agataaaaat
    37321 accgcaatca tcaacaatct ggctactaca actatcaacc atcaagcgat gatgaacgga
    37381 gatgcttcaa tttcagcggc tcgctacaca ggaacagatt taacgactac attgaacctt
    37441 cctcctgaaa aagatccgaa aaaagcgttt gctaccgtga aagatgaatt tgagaaaaga
    37501 tacggtcaaa cgtggttccc gtcctatagt tttgaaaata cgtatgtctt tcttgttcga
    37561 aaagacaccg cacaaaaata ccatctatct aaagtcagcg atctgaaaaa tgtagcggat
    37621 gaattagtag ctggtgtaga tacttcttgg atcaatcgta aaggagacgg ttatgacggc
    37681 ttccaagaaa cttatggctt ctccttcaat tcgatattac ctatgcagat tggacttgtt
    37741 tatgacgcag tcgaagcagg aaaaatggat attgttcttg gctactctac ggatggacgg
    37801 atcgccagtt atgatctagt catgttggaa gatgatcgtc aattcttccc accatatgat
    37861 gctgctccag ttgtagacaa caagatatta gctgatacgc ctcatttaaa agaagcctta
    37921 aataagttag gtcataccat cagtacggaa aaaatgcagg aattgaacta cgaagcagat
    37981 aataatctag tggaaccatc agtagtagca caacgtttct tacaagaaaa ccactatttt
    38041 gaagggaagt gaagcaattg gataaaatga atgtctttca acaatttttc tattatttcc
    38101 aggaaaatgg ctcctatatc tttgcacaat ttatccgtca cttcctgatt tctatctatg
    38161 gtgtcctctt tgcagcagtt gtcgggattc cggttgggat catgatttct aggagacgta
    38221 aattagcaaa ctgggtgatt cgtttggcga atatcatcca gacgatccct tctctagcca
    38281 tgatctccat tttaatcatt ggattaggcc tgggagtcaa tgtcgtgatt gttactgtat
    38341 ttttgtattc cctcttaccg atcatcaaga atacgtatac cgggatgatc caagtagaca
    38401 aaaacatcct tgatgtcggc aaaggcatgg ggatgactgc tagacaacga ctattcatgg
    38461 tagagttacc actctctgtt tctgttatca tggcaggcat aagaaacgcg ttagttgttg
    38521 ccatcgggat aaccgcgatc ggtgcattcg tcggtgctgg tggtcttgga gatatcatta
    38581 tccgaggaac caatgcaaca gagggcacat cgattatttt agcaggtgca ttgccaactg
    38641 cattaatggc gatcatcacg gactggcttt taggtatttt ggaaagacgg ctagatccag
    38701 ccagccgtac atcacgctga tgttattgta aaaaacaaaa gcttctgcga ccatttgttg
    38761 gtcgcaaagg cttttttagt tctgtcattt ttaaggcaat aatccactct gattcttccc
    38821 tccttatggg taaagagttg cttttcttag ctatttatcc attccgtata ctccaacttc
    38881 atactgccaa cgggttcata tctgcatcca ccgactaagt ttttttctta tccgctaagg
    38941 cattttgcca atccattttc ccatcactcg tcatggaatt cataccacat gatctctaaa
    39001 tactcgtctg cttgttcttt gaattgggca tcaattctta catcctttgg cgtggctttc
    39061 atttaagcct tttccatcag tcctcgattg ttcgatgcct tcagctgcat catcaaaact
    39121 ggtgactgcc gtatcggtaa cttcgatcat cttttataca atcgggaact tgccatcctc
    39181 atccttggct aaaaacatat ctagccattc cagcttctcg ccatgggagg tttgcggttc
    39241 ctaaaaccgg tacacgattc ttatggactg catccgtcgc aactagactt ggcgatacga
    39301 tgatccttca ccagaagagc acccccgtaa accaattgat cgatatattg ccaataagaa
    39361 aagacattgg cgtaaaagat atcgatccct cgtgatgcat tgccgttgat actgctattc
    39421 atggccgaaa gcgccacgac tttaacttct tccgtttgag tgacattcat gttcggcaat
    39481 gtcgctttgg atacccgctc tgctaaagga acgacactgg tattgtattt cgtatctgta
    39541 tcttttccaa aagaccatgc caacgaatcc tctgggaacc aataagacga ctccggttga
    39601 ttgtccattg cttttttcaa catacttttg gctgtttctc gataaacgac atttccaatt
    39661 gtcttcgcct cactttggcg ataatcgatg gtcgttgcca ctgccaacac taacaacgat
    39721 gctccaattt tactgatttt ccttgtcaca cttcttcctc cttgttttac ataaacccgt
    39781 caacattcca ttcctaaact caaacaatga cccttcaaag gtggagatat aggaacaaat
    39841 taaagaaatc atgcaataaa tccaagaagg ttggttttag aacaaaaaaa gagtctcctt
    39901 tttggggaga ctctttgtta actatctaaa aaggttggct cttctcactt cactcataat
    39961 gtgtccaggc agaataaatt tctacaactt tattttcctc atcaactttg taaaccactc
    40021 gatgctgtct attcagcctg cgtgagtatc tgccttcatg tgtaggtctc agcttttcaa
    40081 aagactgcgt gggcatatat ggatcttcct tcaatgtctg aatcacttct tcaaattgtt
    40141 tctttaaatt cgtttgcttg atttttctta aatccacttt cgcagagttt ttgattgcga
    40201 ctgtatagtt actcatcaaa gagcttccca atcaatatca tcgatattcg taaagctact
    40261 gtcgtctttt tcgcggtctc gtactttatc taaagtacct gtttgctcaa gaaaaagggt
    40321 ttcttgaatc gctctccagt cttccagccc aatcaataca gcattgtttc cactacgatc
    40381 actaataatc tcgatttcag tatgattttc atttacctct ttcaataact gataaaaatt
    40441 tttacgagca gtgctaggat tcatcgttgt attagccatt gtctctcctc ctttttctga
    40501 tataattata gcgtacttga ttaagtacgt caaaaaaata caataaagca cttaaattca
    40561 gcaaactttt cctctttttt acatagagaa aaagacccct ttttagggag gatctttgtc
    40621 cacgctcact tcttactcct ttacgactag cggtagccaa acgtaaagta attggtaaca
    40681 acgtatcatc tttagcaaag gtcgaatcca aagcctgtga gtctatgcaa caacaccaat
    40741 acacgaaggt tcctcgttgc agtgtgactt gattgatatc ttgaataacc tgaggatacg
    40801 cacgaaagat ttgtaacggt tattcaaaac tgattttggc cgtcaagtct tctactgcct
    40861 caaaaatgat tgcctctttc gtctcctgtc attcatgttc actatcgatt tgatacttgc
    40921 ttagaccgtt gggtttgtga atcaccaatt aaaaatggca ctgagttatg gtaacaaaaa
    40981 atggtcactt gacgaggctg agattcctac ttatgaagac acatggcact taagagtcga
    41041 ggtaaaggaa gaagctgaaa atataggagg ctgtacatta tttggcaaag gattcggaaa
    41101 ttatgatcaa gacatcaaaa tcaaaatgtt ttacagctaa ataggaagaa taataacatt
    41161 tcatacgctg ttcccatctt taatggctta tagagggcac ctttagctga gaataagaca
    41221 aaacagaccc tctagcttca attaagagct agagggtctg tttttgattt taaaacttgg
    41281 tttatttctt caaatggtag ctataggtgc tgacaacgat attttcacgc caagacaatc
    41341 gcaatcttaa ccgcaaactt gtttggttat ggagaatatt ctgccagcgt ctgtttggta
    41401 caaattgagg aatcaacaca gtagttgtat agttttgttt cgctgcattt ttacttacga
    41461 tatctacata acggataatt ggattttcaa tcgagcgata agaagagtgg acgattgaaa
    41521 aacggacatc tgggaaatgc tttttaaatt ctgcttcgat ttctttttct ttttcgacat
    41581 cttcgtctag cgaaacatgc atcgcaacga cataatcacc gatcgaacgt gcgtaattca
    41641 acgcaccgat atttacccgt gtcacattcc cgactaatac aagaacggta ttcccgtcat
    41701 aatcatgtag ttttgcatca ttttctaaac gaagctgttc tgctactttt ttataatgat
    41761 catggatctt ataaaaaatg aacataacga ttggcataat gatgaagaaa ggccaaatgt
    41821 cccctaaccg atagacaaac aaaatcgcaa tgattgcata tgagatgaac gcacccgtaa
    41881 tattggcaat cgatttgctt aaccatcttt ttccttcttt tttccatttg acgaccatcc
    41941 ctgtttgaga taaagcaaat ggaatgaaca cgccgataga gtataacgga atcaagcgtt
    42001 ctgttgagcc atggaaaata aacagcaata cgatcgaacc tagcgctagt gtgataatcc
    42061 cattggaata gcctagtcga tccccacgat cttgatacat gtgaggcatg aatttatctt
    42121 ttgccagatt gtatgcaaga actggaaaag cagaaaaacc ggtgttagca gcaactgcta
    42181 aaatcagcgc tgtagcaaat tgaaggacgt aatacaaaat accatgtcca aagacagctt
    42241 taccaatttg agacaaaaca gtgacttctt tttcaggaac gatcccatac caatagttga
    42301 taaatgtgat ccccacgaag aagaaaccta agatcaatgc catcatcgtc aatgtagcag
    42361 cggcattttt tgctcttggc ttcttaaaga atggtacagc attactaatc gcctccaccc
    42421 cagttaaaga ggaagaacca gaagaaaatg ctcgtaaaat caaagcgata gaaacacccg
    42481 gcacagccgc tcctggaagt gcagtagctt gcaatggcac agcacctgtc acgatattat
    42541 ataaaccaac aacgatcagt aacgagatca caaggataaa tgtgtacacc ggaaataata
    42601 ggaacgatgc agattccctc agcccacgaa gattcagcat catcagtagc aaaacgattg
    42661 tcacagaaat agcgacttga tgaccgtaga gtgctggcac cgctgatgtg atcgcttcag
    42721 cacctgctga gacagatact gctactgtca acatgtagtc gatcaataaa gagccaccgg
    42781 aaatcaagcc agcattcttt ccaagattct cactgctgac gacatatgct cccccgccat
    42841 gaggataagc atggatgatt tgacgatatg atagcgttaa tgaaatcaat aaaataataa
    42901 caaaagcggc aatgggtagc gaataccaga tggctgcagc agacaaagcg actaaaacga
    42961 caacgatttg ttccgtacca tatgcaatag atgataacgc atcagacgac aataatgcca
    43021 aagcagcaaa tcgggttaat ttgtgctcat cattttcagc agattttaaa ggcttaccta
    43081 ctaacaacct ttttaagtaa tccaatttct aaactccttt acacacaatt ttacttcaag
    43141 ttttatagtc gaaaatttat agtaaacaag atgttatagt acgctcactg accttaaaag
    43201 tcaataaact ttaagaaaat aattggaaaa aaacagaaaa atatcaagcg ctttttcaaa
    43261 cgctttaata cagcaaaaat ctaacatttt tttatgggct ttttctttga attctataat
    43321 aaaaggatag aacttgactt agaaaatcaa gttctatcct ttttgatatc ttaacttttg
    43381 gaagagagat gtaaggctgc tttctaccca tcattcttac tactcgaagc atagcgcagt
    43441 tgtcacaacc tcttgataaa cggtgtttaa aagcttgcat ctgcggtaat aagcccaacc
    43501 aaattaaaaa cataagaagc tgtttttatt tggtcgttct tattacttga tgcagagcag
    43561 cttttttaca acctcttggt acacggtgtt caatcagctg accctcggta ttagctcaaa
    43621 aaaacggcaa aatatgagga actatttttc catttttttc tctaatactc aggtcaaaac
    43681 actgatttca caacctcttt ttttaatgaa aaatatggct ctcatgcgcc gcttctttga
    43741 atttcgtcat tttttcttcg atgaacggat agattgccgt tcctacgatt ccaaagacaa
    43801 caaacaaacc gatcatgatc cagattgctt tcgtagcatt tggccaataa attcctcctg
    43861 cagattcccg taacaagttt accgcatgag taaatggcaa gaacggattg atcatttgga
    43921 agaatttccc tgagacttgg atcggatagt tcccgcctcc gccagagatc gacagtacta
    43981 agatgataat agcgatccct ttaccgacat tatcgaataa tgcagccaag acatagacga
    44041 tcatcatgaa cgctaaggcg atcaagacag caaatgacac gctgtagacc ggattttgga
    44101 catctactcc tagtaagaaa atattcccta atgtaacgat caagctttgc gcaatcgcca
    44161 ttgtcaaaaa tgtcagcata cgagcaacga attgttctcg tttcgtaaaa ttgtttcggt
    44221 ctttctttcc taaatagtat tctgtcgtag caacacttga aagaagaaca gcgccgaccc
    44281 acagacatag tgcagtataa aacggggtgc tagctgaacc gttatttgcg attggataaa
    44341 gcgcattggt ttgtagctca acaggttgtg taaagaagtc actttctttt gttgcgtcta
    44401 gtttcaataa tttgatgact tcgcctagat cgacttcttg ttcgcctttt cggatcgcgt
    44461 ctgctgcttt ttggatacca gctttgatat ttggccagtc attgttgatc aagtccgcag
    44521 cagcgttcac agctgtttcg acttctggca ttttttcatt tactgttgtc aatgtaccgg
    44581 tgatttcatt ttttagtgct ggccaatcat tttggataaa atcagcggcc aatcctaatt
    44641 tttgctgaat gatcggtaat tcattgttgt ataggtctgc cccttcattg atgccattga
    44701 cgatggtatc catatttcca ttcagcattg tattggcatc atggatttct tgtccaattg
    44761 caggcatttc tttttgatat ttttctaaaa ttgccactgc attcgttaca gttgctttcg
    44821 ttgagctgag aagcgaatca aagtcgatct ggtccgcttt gctcagtgcg ccttgtgcag
    44881 tcccaatcgt atcgatgact tggtctagta gagaagtgac agtggaactg atttgacctg
    44941 tattgatacc agcaatgatg ccattgatat tattaacaac ggcatcaatt tgatctagca
    45001 attcatcaat agccttcgtg tcttcatttt gaacagctgt gttcaaagca gataggcggt
    45061 tttgcagatt gcttagcaag ggttttaatt gatttagctg atcaataatt ggctgtaatt
    45121 tcgtatttcc agcacttgtt tgcaatttag tcaggaactc gaccagttta tcgattgcat
    45181 tttgttgttg agatattccg tcaataaagc cgttcaatac attgctgatg tttttctttt
    45241 catcatcagt cagcttacca tcttcaatag ctgttcttac agtatctatt aacgaacctg
    45301 tcgttgttgc cacttgtcca attgacttca atgtaacatc tacactgttt gtgatgcttg
    45361 gtatcgcatc tttcagctga gaggcacctg attttgtttg gtttgctaga tcacttgcct
    45421 gattttctaa atttttcaca tctggcaaga tatcttgtac ttgttggatg atcgtcaagc
    45481 cttgttttgc ttcatcgatt ccttggttca ttgtctcttg gatcgaagca aaatcgccgt
    45541 cgatctctgc tagttgctta ccggcatttt ggatctctgg gattttttct tgcaaatcaa
    45601 ggatgatttt tgcttgatct ttcaactcag gcattttgtc attcaatgca acgacttttt
    45661 cacccatctc atctacttta gggatgtaat ccacgaattc atttgcttta ttcagttttt
    45721 ccttgatttc aggtagttga ctttgtaatt cgaggacttg ttttgtgtag ccgtcgatcg
    45781 tgtccaaatt ttcatctgtt gataaaatca tatctttaac tttattgata cttactaggt
    45841 tcgtatcgat gtcgtaacct atctcattga atactttcaa taaagtcgaa ctggctgttt
    45901 tgataaattc atttgtgatt tgcgattgga tcgaacttgc tcctttatcg gtgattttcg
    45961 gtgcaatcgc attgattttt tcatttaccg tatattcgat ttttggtttc ttgatatcac
    46021 cactagtaaa actcagcaga tcttctgaga aatcctttgg caagtaaata cctgcgtaat
    46081 actttccgga acggactccg tcttctaatt gttcttttga atcaacgaat tgccatccta
    46141 attgtttatt tttatgaagc gattcgatga cttgctcccc aatggccact tctttacctt
    46201 gaaactctgc tggcttgtct gcactgtaaa cagcaattgg cagctctcca gtatttccat
    46261 atggatccca taatgctttg atgttaaacc acgcatatag cgaagggatg atcatgatcg
    46321 cgatgatcag aagtgttgca atcgggtttt tgaaaatccg tttccagtca agcaaatata
    46381 atcgaagcgt atttttgatt gactgcatag ttctccccct tcttcttttt tcagctgaat
    46441 tactttacca tgtatttcat acttttttca atcggctttt ttcaaaaaag tcgaattttt
    46501 ggacatatca cggcaattgt ctcattgtaa tctcttacat attgagaaat aattaaggat
    46561 tacagccttt actttatcat gaaataaaag ggaaagataa ttttatgcca aagtttttat
    46621 ctattatacg tatacgcaaa aaagctaaaa cattttttgt ctttgcccct acaaaaatgt
    46681 ttcagctttt ttcgtttttt attcctgtat ttcttatttt tcgaatgaca ggatgatttt
    46741 cccagcagga ggatgtcctt caactagctg atgtgcttgg atgacatgct taagtgaagc
    46801 agacagattc attgaaataa aaacatgaaa ggctcctttt tggtaggctt tgattatgcc
    46861 agcaaaggct tctgcatcta gatattcctt tcttggaaca aacgattcat aaaagccttc
    46921 ctttttttgc cgcaaatcta gatctgggag gtcatttaat gcaacatacc tgccgcctgg
    46981 tttcatgatc tggatgccgg tttctccttt gatacttcct ttcgtcgcat cgattacaag
    47041 gtctgcctga tcagcaaatt gcagacctgg atttgtttta tcataagcag caaatgctga
    47101 agctcctaat ttttttacct tttcctcatt tttactggat gcactggtta agattcggat
    47161 tcccttttca tggagaagct ggatcagact tgacccgacc gcacctgatg ctcctagtat
    47221 cattacagta tctgtcggct ggatttccgt caaatggttg attagattat aagccgtaat
    47281 tcctggcgtg accattccgg ctgcttcttc ccaactcatt ttatctggaa tttttgctac
    47341 ttttgctgat ggcaacacga cttcttctcc gtaagtcccg ccgactgcat ggacagctac
    47401 tcgatcacct acatgaacag ttgtgacatc tgaaccaatc tctgtcacaa taccagcgcc
    47461 atcattacct ggtacataag ggaatttcaa tgttcgtatc tctttcattt ctcctaaacg
    47521 caaggcaaca tcataaggat tcacactgaa tgcttttatt tctacacgaa catgtccaga
    47581 accgacttct ctgggatgag catcgatttc ttcaaaaaca tcttgtgctt ctccgtaacc
    47641 gttaattgca aatcttcgca tggtcaccat ctcccttttt ctttaagttt acgtttatgc
    47701 ctttattttg acaaaaaaaa tgtcttttcg ttttggcgca tgattcaaaa caaaatagtt
    47761 gtcttttttc gcgtaacgtg ttaaattatt tatctaaaag cgtaaagatt acgatttacg
    47821 ctaataaaaa ataattggag gaatttgttg atggctaaaa aatcaaagat tgccaaagca
    47881 aaaaaacagc aagaaacgat tgaacgctat gctgctttac gccaggaatt aaaagcgcaa
    47941 aaagattacg aaggcttgcg taaacttcct ttagatgctc gtccagaacg attgaaaaat
    48001 cgtgatttaa tcgatggacg ccctcgcgga tacatgcgta aattcgggat gtcccgtata
    48061 aaattccgtg aattagcaca taaaggtttg attccaggag taaaaaaagc cagctggtaa
    48121 tccaagtctg gctgtaatgt aaaggaggag ggcgcatgga ccactccttg gatcatcaaa
    48181 aaagatacgt tcgcttcttc tagcagaaat gctggtacta gaagcagacg tatctttttt
    48241 tacgcacagc tggtcctttt ttatatgtaa aggctgcatg gttacttcgc tatatttatt
    48301 tagtaacagg agtcagtgct tcgataccca cactaatttt caatgctcgg tcaacttctg
    48361 ccataatggg tcgatccaaa tggcagacct tttctttcaa tcttgattta tcgatcgttc
    48421 tgatctgctc caacaggata acggagtctc tttctattcc tgttccagta gctttgatcc
    48481 caatgtgagt cggaagctta ggtttagcca tttttgccgt tatggccgca acaatgatcg
    48541 tcgggctaaa atggttacct agattatttt gaataaccaa aactgggcgt gttccgcctt
    48601 gctccgatcc cacgactgga gaaagatcag caaaataaat atctcctctt tttaccatag
    48661 gatcctcctt attcttgata ttctcttggt atccgctgtc ctaatccgca agccacttca
    48721 tagtggatcg tacctaattg atctgctata tcctgcatag tgatttcttt gtctgcattt
    48781 ttgccgataa gcgtcacttt tgttccgaca ggaaattctt gaggcaatcg gatcatcagt
    48841 tgatccatac atacacgccc aatcgtttca cagtagttcc cttctaccaa tagagaaaaa
    48901 ccctgcatct ttcgtggcca gccatctgca taaccgatcg gtatcgttcc gatccactct
    48961 gcttcaggag tgatatatgt ttctccatag ccgattcctt cacctgctgg gagttttttg
    49021 acttgaatca attcagaaac cagctcaagt gctggctgca atggataaac ttccggtagt
    49081 gcgtgcccag agggattaag tccgtacatt gctactccat aacgaatcat attgccgatt
    49141 gtttcatcat gccataaagc cgttgcacta ttgctgacat gtacatatcg aggaagttcg
    49201 ggaagttttt tcagcacttc tataaagcgt tctttttgca gattccaata tgtatcatct
    49261 gcctgatctg ctgtagaaaa atgggtaaat aaaccttccc attctaatac acgactttct
    49321 ttgatgaatg cagctgcctc ttttacttct tcaggtgtac aaaatccgat acgacccatc
    49381 ccagtgtctg cttttagatg aatcgaaaga ggcgtctcta gcttactttt ctctaaaaca
    49441 gcagctgctg cttgcaacca ttcccgtgta cctgcagtta ctgataaatc atgagcaaca
    49501 gctacagtca gtgcgtcgta aggaaccata ttcaagataa ggatgggctg agtaaatcca
    49561 gcttctctca actcgactcc ttcatctaaa tttgaaacac aaaagcccga agctccacct
    49621 gcaactgctg cctcagctgt ttcaatagct ccatgaccgt atccgttcgc ttttactact
    49681 gcaaacaatt cttttccttg cggaagtctt gcaacttcat ttgctacatt ttcagttata
    49741 gcttttttat gtatcactgc tttcgttggg cggtgccaag cgacaaccat cgtttttcct
    49801 tctttctagt cttccaaaat aatttgtgca aaagctgttt catttgtatg agtaatactg
    49861 acccatacat tgccttcatg aggtgaacgc gtcacaacag gctgaccatt ttcatttttt
    49921 aaaatctcga catcttgaaa ggtaactttt ccgatcccag ttccccaagc tttagagaaa
    49981 gcctctttgc aagcgtaacg cccgcctaaa aattctactt ggcgatgaaa aggcaaggac
    50041 tggaatagtt tcatttcatc agaggtcaag attcgtgcaa tgaattttgg tttttcttcg
    50101 atcagccgag tgatcctagg aagttcgact gcatcgatcc caattccttt tatcatatgc
    50161 ttttctcttt tcaataaatt tagaacactc tcattctacc aaattataac cattttgcca
    50221 caaataagtc cacaaataac aaaaatgaaa gtttctctta aaaaagagaa actttcattt
    50281 tatgtttaat cgttatttga acggattaca aatccgcgtt ttttatcatt gtgacgttta
    50341 ccgccatctt tttggctgtt attatttttg ctataaccgc cttttgattt gtttccacga
    50401 taattaccgc cgtcacgacg attacggttg tttccgccac cacgtttgcc atttttattg
    50461 tatccttttt tctgcattgg caatgggcgt tctggtgtga tcttcactgg cactgcatca
    50521 gctggatctt ttgctgtcgt ttttagcaaa agtgcaacta aatcttgtgc agaatattct
    50581 tctaataatt tatctgctgt ttgcaggtat ttatctagac cattttctgc aagttttgtt
    50641 tcgatttgtt caactgcagc acctaattga cctttgaatg cttctttttc ggttggtgga
    50701 cgtaaagtcg tcatccgttt tttcgtcaag ttttcaatca catgcaagta gtccatttca
    50761 tttggtgtaa cgaaagtcac agacataccg ccttttccgg cacggcctgt acgtccgata
    50821 cggtgaacgt agctttctgg atcttgaggg atatcatagt tgtacacatg agtgacacct
    50881 gaaatatcta atccacgtgc agcaacgtct gtcgctacaa ggatatctaa atgaccgctc
    50941 ttaaatgaac gcaatacgct cattcgtttt tgttgagaca aatcgccatg gattccttct
    51001 gcacggtaac cgcgtgcttc taatccgcgt gccaattcgt ctacacggcg ttttgtacgt
    51061 ccgaagacga ttgttaattc aggtgtttga acatcaaaca agcgagtcat gatatcgaat
    51121 ttttcgtatt cttttgcgcg gacatagtat tgatcgatca agtcagctgt catttctttc
    51181 gctttgatct tcacatgatg tggatttttc atgaatttaa cgccaatatt cttgatagct
    51241 ggcggcatcg ttgctgagaa aagtaatgtt tgtctttgat caggaacttg tgaaatgatt
    51301 ttttcgatat cttccaagaa gcccatattc aacatttcat ctgcttcatc caaaactaat
    51361 gtttgaacgg ttccaagttt caatgtgtgg cggttaatgt ggtcaagcat acgtcctggt
    51421 gtacccacaa cgatatgtgg acgatctttc aagccacgga tctgacggcc aatatctgct
    51481 ccgccgtaaa cggcttgtac acgtactttt ttgtcgcgcc ctaaacgata taattcttct
    51541 tgtgtttgga tagctaattc acgtgttggt gcgataacta aaccttgtaa ttcgtgacga
    51601 tctggatcga ttttttctaa cattggtaat ccaaatgcag ctgttttccc tgtacctgtc
    51661 tgtgcttgtc caataacgtc ttttccagct aatgctagtg gaatcgtttc tgcttggata
    51721 ggcgttgctt cttcaaaacc agcgcgttcg actgatttca acaattctgg tgataattct
    51781 agttctttaa atttcaaatg aatcctccta tatggttgtt aactgaggat gtagcttttt
    51841 tgctaccccg tcgatttgtt cggtcaaagt gtattgtttc tttttgacag tggccacgca
    51901 cgtttttatc cattcttagc ccctgtggca ccaccgaaaa atcactttgc gaagtttagg
    51961 cattagaaaa gccaaacggg tcgcgttcag ctttatcatt attatgacat ctggcttcaa
    52021 caagttgttt ctatggcaaa accagtccaa tttctcttca tctttcggca ataggttatt
    52081 ctaacatagt ttttcgtttt ctgcaagttt acagattttt atttcatttg ctgaagttgt
    52141 tctaccactt caaataagcc catgctgttg ctgcctttta gcataatact atctgttgct
    52201 tcaagatttt gtttgatagc atcaatcaat ttattctttt ctttttcaaa atagaacaca
    52261 ggtagttggg ggtatttttg gttcaattct tcttttagtg catgcatttg ttccccataa
    52321 aggaaaatca tggcgaattt ctcaggatcg atatgttcac tcatttgcgc atgcatattt
    52381 aaagactctt tccctaattc cagcatatct gctaacacag cgattctgcg tccttttgtt
    52441 ggcagatttt taaaagtatc taataccagt cccatggctg ttggatttgc attgtagaca
    52501 tcgctcaaaa tagctgcacc atttcctgcc gttagccatt cggttctgtt ttgagtgata
    52561 gaaacatggg cgagcccttg gcgtatctca ggtatggaca gtccaaagaa gcgcccgatc
    52621 gtatacgcaa tcaaagcatt cgttacatta taacttccag gcagcgggat cgtaaatgtt
    52681 tctccttcta ctacaaaatc ggtttgtgct tttccttttg ctgtcacttc cgcatgaaca
    52741 tcgccatttc caataccgaa tgttgtgact gtttgtacta atgttgccgt taaaggtgtc
    52801 agcaacggtt catctgcggg cacaacgagt aatccgtctg atacaagtcc gtcagtgatt
    52861 tccattttgg ctttagcgat tccttctcgc gacccaagat tttctatgtg tgcttcaccg
    52921 atcattgtga ttgctgcagc ttttggttga gccataagtg aaagttccgt gatttcattg
    52981 gcgtgatcca tgcccatttc caaaacgatc ttttctgtat cttggggcat gtgcaaaatc
    53041 gtgtaaggaa gaccaatatt attgttgtaa tttccttgcg tcttatatgt tttgtatttt
    53101 tcagacaaga cagacgcggt tagatctttc gtggtggttt taccattgct tcctgtaatt
    53161 gcaacgactt ctgcgcctac tttttccaag taataggttg ccaattgctg catggctttc
    53221 aaaggatctg ctacaggaat aactggaaaa ttttttggag cttgttcaac aggcaagctc
    53281 caaaatgaag cagatgctcc gtttgtgatc gcttgctgga taaattcatg tccatctcgc
    53341 gtaccagcaa gaggaacgaa taaacttcct tcggttatct tccggctgtc aaattcgaca
    53401 ttggtaatct ttgtcagtgc agagcattcg ctgcccacgg cttgtgcaat ttcttgtatt
    53461 gttagattca ttgagatctc tcctaaaaaa ggttgtgaaa aaagcgtttg gcctaaacgg
    53521 cgggttcgca tgactctcac ttccaagtaa atttctctat tcgtatttag aagctagaat
    53581 gaacgcggtt acgcaatcac tccagcctct tctttatttc tctttattca tttttttcaa
    53641 aaaaagattg acgctgatgg tatcgattca ttcctaactg gatcagttct tcaatcaaat
    53701 caccgtattt caagcccata ttttcccata agagtgggta catactgaac tccgtaaatc
    53761 ctggcataga gtttaattca ttcaggaata attcattttt atttgtcaaa aagaaatcgc
    53821 accggctcaa tccgcttcca cctaacatcg tgtaagctaa cttcgcgtac tcttgcgctt
    53881 tttgataaac ttcttctggc acttcggctg gaatctgcat ttcgatttta ttattgatat
    53941 attttgcttc ataatcatag aatgctacgt cttttacgac ttcaccaggc aaagtcgtcc
    54001 gaacgtcttc atttcctaat acagcaactt cgatttcgcg cgcttcaatt ccttgttcaa
    54061 cgattgctcg agaatcatac tgataggctg ttgctaaagc attttgcagc tcttctcggt
    54121 tttctgcttt tgtaatgccg acactagaac ccatattcgc cggtttgaca aacatcggat
    54181 aaagcaaaga accttcacat tgatcaaata cttttttagg attttctttc cattgattct
    54241 taagtactgg tacataagga acttgcggca caccagcagc ttgtaaaata tacttggtca
    54301 tgattttatc catggcacat gcactggtca atacgcctgc gccgacataa ggcatattca
    54361 atgtctctaa gaagccttgg atcgttccat cttccccgtt tggcccatgt aaaactggaa
    54421 aaacgatggc tccttcttct ttgatttcgc ccggattgat cacttttcct gtaaagcctt
    54481 cctctgtctg tccacttggg tcccatgaaa gatgcaagac atctttgctg gcaggttttt
    54541 ctgttagtaa tggacctttg acccattgtc cttcttttgt aataaatacg agttgaactt
    54601 ggtaataatt ataataaatg gcatttaaaa ctgaaaatgc ggaaagaatg gacacttcat
    54661 gctctgcgct gcgtccgcca tatagtaaag taatcttcaa aagattgtcc tcctgtattc
    54721 taaatcgttc agtgatttac tctcatattt tagcacaaaa caaaaataaa cgaataactt
    54781 atttataaaa aaacgagcca gcttctaaaa gaacgagcgt catttatttt ttgtcagcta
    54841 gattatttgt cccgtttttt ataaaaagtt atgataaaga aaaggaggga tgatgatggc
    54901 gcaaacaaat ccacgcagcc gcttggcaga aatcattcga gtgttcatca attacaatgt
    54961 agtgccaaac tttgtacagc aaaaaaatcc cgaacaggta aaaaaagcct ttgaagaact
    55021 aggccccacc ttcatcaaga taggacagat gctttctgtt cgagaagatt tgctttcttc
    55081 tgccttcact caaacattca aaacattaca agatagtgta cctagcgaca cattttcaac
    55141 ggtcaaaaag acgatcgaaa cagaactttc tctttcatta tcagatatat tcgatgattt
    55201 ttcaaaatcg ccttttgcct ctgcttctat ggggcaggct catcgtgcca agctgaagaa
    55261 cggtgacgcg gtagtcgtta agatccaaca ccctaacatc gccgaagaga tccgacttga
    55321 tcttcaacta tttgaacgag cgatcccttt gatcaaatac attcctgaaa ctagcgtagt
    55381 tgatctaaaa ggggtactcc aagaagtaaa acgttccttg atcaatgaaa tggacttctt
    55441 aaaagaaagt caaaatggcg aacaattcta tcaaaagaac aatggctgga aagagatccg
    55501 cagccccaag atttatgacg ctttttgttc gaaaaaagtg atcgtgatgg aagaaatgtc
    55561 tggtaaaaac ttgaatcatt taatgaacgc cgaaaacaaa actgaaactt ttatcactgg
    55621 aattcaaaac aagcagctga aacaagaagt tgcaaagctt ttagttgaga actttatgaa
    55681 acaggtgttt gatgatggtt ttttccatgc tgatcctcat ccagggaatc tgctattcca
    55741 tgtactaaca aaagaagaac agacacaagc tagccgaaaa actgaaacag tccatgaaaa
    55801 agagttcggt tcctttgctt ttcgagcctc cacttctgca gaagaccctg ttgcgccata
    55861 taccatcaac tacatcgatt ttggtatgat ggggcatctt tctgcaggac ttaggcaaaa
    55921 actcacacaa gccgttttag cgctgtatac gaaagatgct tatcgaatcg aaaaagcggt
    55981 ccttcgactt tgtcagcaag aaggctcgtt tgatgaaagt cggttccacc aagaactgac
    56041 aagttttctt gaacagtatt atgattctcc tattgatgaa atcaacttgc aagaagtatt
    56101 cacacatgtc gtgacgattt gtcatcaaaa taatctgcag ttcgatcgtg atatcacgct
    56161 tttgctgaaa gcatttggga cattagaagg ggtgatccgc gtcctcgatc ctgaagtttc
    56221 cctcatggaa gtcgcttcgc cttttgctca acattatttt ctcactcatc tggatgtaga
    56281 agatacattg aaacaaagcg gattagactt attggaaggg atgaaggctg cgcctaagat
    56341 ccctcaacag ctccaccatc tattagaaat gtggacttcg ggacaaggaa aagtcaattt
    56401 ggaattgaaa aagcaagaca aattattgag caggatcgaa agtatgatca atcggcttgt
    56461 tttcggtatg attttagctg cattgatcgt agggtcctcc ctgcttgtcc aagcagcacc
    56521 tgtagaaaac gcagaggtcg tttccttatt agggatattt acgtatgcta ttgccgcatt
    56581 cgtcattatt tttttggcaa tcgatgcatt gatccaaatg tataagaaga ggaaaaagta
    56641 gaaaaaggtt gtgacagaaa gaatcagctt caagaaataa gaaggggttt tacataaatt
    56701 gtttctcaaa tttttgtgaa aattcggctt atttttcgaa gaggctactt ctgaaacacc
    56761 gagaataaaa aatgtgaaaa agctgttctg cacccagtaa taagaacaat caaaaaggga
    56821 acatggaaca catttatcct gtttccctgt tccctttttg atgtattcga gcataaataa
    56881 aataccttcg ttacctacta tcttctgtac ttggtaaacc ttagtcaatt gcctttttta
    56941 acgcttgacg aaaagacata tgatctttct ccgtaaaggg ttttggaccc tttgtcctta
    57001 tgccagcttt tcttaacttt ccactatgat aacgttgttc tagaagcgtg ccgatattcg
    57061 cttcgtcaaa ttcttttcca gagtgatgga acactcgcac acccttcggc aatacaagtg
    57121 aagctaaacc ataatcttgc gtgatcaaaa agtcattctt cttaaccata ccgacgattt
    57181 tataatccgc actatcagct ccgcggtcta cgtagacgat ttggacatga ttagcgtatt
    57241 ctttggtcgt ataatggtcc acactggtca cgataaccac gtcaagacca taggattctc
    57301 ctaattttat gacatcttct tttactggag aaccatctcc atcaatgaat agcctcattc
    57361 tttttcgcct tcttccataa atgataaaca gttactaatc cgctcagtaa aagaccaaca
    57421 ttgatccatt ttgccaagat aagcagttgt acactttcta tcggcactga ttcatggttc
    57481 agcgtgatca ataatagatg gattggctct cccatttctt ttgctaaaac aaacaaaagc
    57541 gtcatcaata aaaactggtg gatcacactc cctttatttt gagtagtata cttgacaacg
    57601 atccggcgat ccattggaat agctgtatca gtttgataat attccttatt tagccagctg
    57661 cctgttacta cgtgccagaa actaagcaga aagacaccta tcgcaaaacc atttggaatc
    57721 aagaggacca ataaagaaga actcagtcca agcaattgca tagccaatgg agaccatgtt
    57781 aaacgtttca aaatccatgg tccgactatt tttgctgcaa tcaagcctgc tgcataaggc
    57841 aaatacataa aaatcgaaag tttttctaat ccgtacagta tgccaacata aaaactgcta
    57901 aataaaaaaa ggaaattccc tagcattcca tttagaaaag taaacagatt tagccagctt
    57961 ggcaacttcc attccttctt ctggtgtcct aaaacaaaca cgaacaagat gaacagcagc
    58021 aagaaaccat aaatagcaag atcaaaaagc ctttcatcga ataacaatcg ggcatttctc
    58081 aaaagaaaca ataagccaaa gaatatcaaa aataaaacaa gctctcgcgt agctaccaga
    58141 tttcttttga tgtctttgaa atccagttcg taacgcggat aatgactaac cgtatggtag
    58201 gccatgacgt aaaaaagtgt ataaatagta aagcttgctg caatctgagt cggtgcaggc
    58261 aactcaatcg cttgatacag gagaaatagc cagatagctg caaataagta ctttttccca
    58321 tccatattga taaaaccttg cgtcttttcg tgaaaattga ccgtggtatt agcaggtaca
    58381 agccatgctg cactcagccc tagcaaaaca cttgaaaaat aaaataaagg aaaataaaat
    58441 acgcctaata ttccaagcaa tgaaccagca ccaccaatca gcagagcgat catcagcaac
    58501 gtgtaactat ctagaccaaa tcgtagactg ttgactaaaa atagtccagt catccgaaag
    58561 gcataaaaca gcgtaaatgg caaaatcgtc agccaattat gcgagtctgt tagtcccaaa
    58621 aataaaagat aaggaataaa aataaccgtg ttacttaaga taaagggcgt tgtttgtgta
    58681 aaattacgcc caaatcgttt gagtttttcg aacatgacaa tggctcctta aacagtctta
    58741 taactaatct gctcttcgat tttagcatta aaggggaaat aaaccaattt tacagaatat
    58801 attcttgtaa aaaaaaatac agaaagaagc ataagcttct ttctgcattt ttacggtact
    58861 tgattactca agcagacaag cccttctcca caaaagtctt gcccgacacc cctgcatccg
    58921 gcaggtgata aagatagaaa tcaattatcc tgtgcctcca aaatcaatta ggagctctgt
    58981 aaaccagagt ccttctcgac ccgtcgcata aatttacttt aacacagttc ctatgtttat
    59041 gcgagagctt ttttcatttt ataatcgacc atttgaaatc cttgaccgat atctgaaata
    59101 agttcttctg taatctcaaa tccttcatgc ttataaacct cgattgcttg atgattcccc
    59161 tgattcactc gcaaaaagat ctctctttcc tttgtttgcg cagcaagttc ttcataccag
    59221 cgaaaaactg aagaagtcaa tccttgacca cgtactttat ttagcaaata taatttactt
    59281 aaataaattt ttccatttat ctcttcataa gcagtgtaac cgacagtctt ttcttcgctt
    59341 tttaataaaa agtaggacac accttctgtc agttcttttt gaatctgttt ttttgactga
    59401 taagttttca gcatgtaatc cacttgtttt tgaccaatga tcggcacaaa aacttccggc
    59461 catacttctt gtatgactgc catcagctca tccagttcat tttctgttac ttttattttt
    59521 tccatacacc cacgtccttt tttacattat aaatgacctt gcgcttgatg gaataaaaag
    59581 agcatgatca tgctatacac acccaccgac cacggtttca agaaaatcac aatctgcata
    59641 aattccctaa aagataattt cgtcaaacca gctactagac agatcaagtc gtctggcgca
    59701 aagggaagca gcaaggtaac aactagtact tttttaatat ctttcgtttt tcttgtcagc
    59761 aattcatcaa atttctgata cttttttggc gataaaataa gctggacaaa ggctacgcca
    59821 tagtaacgga caagcaaaaa tccgattgct tccccaatca ctaggccaat acaactatat
    59881 aaaagcccaa atccattgcc aaacatcagc atgcccgcta ctgaggagat ccctcccggc
    59941 aaaataggaa tgatcacttg gatcacttgc aacagaacaa atatcatcac tgcatacttg
    60001 ccaaattgct ggataaacgc ttgcaaagac gtgctcgatc gaaaaatccc atgatgataa
    60061 gcataaagaa tcaacacaaa gaaaaaaaac agaccaataa tgggcatgat ctgaagacat
    60121 ttttgtaaaa ctgatccttt tttagacatc tatattcccc acatcttttt tcatttatta
    60181 aaactgcctc ct
//
LOCUS       DAJSBL010000015        57915 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.015, whole genome shotgun sequence.
ACCESSION   DAJSBL010000015 DAJSBL010000000
VERSION     DAJSBL010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 57915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57915
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.015"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            273..839
                     /gene="amaP"
                     /locus_tag="ONT85_001020"
     CDS             273..839
                     /gene="amaP"
                     /locus_tag="ONT85_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HCR2378014.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            852..1040
                     /locus_tag="ONT85_001021"
     CDS             852..1040
                     /locus_tag="ONT85_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HCR2378015.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            1053..1610
                     /locus_tag="ONT85_001022"
     CDS             1053..1610
                     /locus_tag="ONT85_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HCR2378016.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            1642..1884
                     /locus_tag="ONT85_001023"
     CDS             1642..1884
                     /locus_tag="ONT85_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HCR2378017.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
     gene            2225..3439
                     /locus_tag="ONT85_001024"
     CDS             2225..3439
                     /locus_tag="ONT85_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HCR2378018.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
     gene            3470..4897
                     /locus_tag="ONT85_001025"
     CDS             3470..4897
                     /locus_tag="ONT85_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR2378019.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            4920..5846
                     /gene="glsA"
                     /locus_tag="ONT85_001026"
     CDS             4920..5846
                     /gene="glsA"
                     /locus_tag="ONT85_001026"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HCR2378020.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            6054..7514
                     /locus_tag="ONT85_001027"
     CDS             6054..7514
                     /locus_tag="ONT85_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2378021.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIRGIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            7735..9498
                     /locus_tag="ONT85_001028"
     CDS             7735..9498
                     /locus_tag="ONT85_001028"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HCR2378022.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            9582..10040
                     /locus_tag="ONT85_001029"
     CDS             9582..10040
                     /locus_tag="ONT85_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HCR2378023.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            10149..10832
                     /locus_tag="ONT85_001030"
     CDS             10149..10832
                     /locus_tag="ONT85_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HCR2378024.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            10979..11593
                     /locus_tag="ONT85_001031"
     CDS             10979..11593
                     /locus_tag="ONT85_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HCR2378025.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            11609..11815
                     /locus_tag="ONT85_001032"
     CDS             11609..11815
                     /locus_tag="ONT85_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2378026.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
     gene            12354..12911
                     /locus_tag="ONT85_001033"
                     /pseudo
     CDS             12354..12911
                     /locus_tag="ONT85_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
     gene            12969..15008
                     /locus_tag="ONT85_001034"
     CDS             12969..15008
                     /locus_tag="ONT85_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HCR2378027.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKIAFDSLGGKAASTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKMITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSFKTTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(15117..16268)
                     /locus_tag="ONT85_001035"
     CDS             complement(15117..16268)
                     /locus_tag="ONT85_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HCR2378028.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(16422..16952)
                     /locus_tag="ONT85_001036"
     CDS             complement(16422..16952)
                     /locus_tag="ONT85_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HCR2378029.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(17051..17401)
                     /locus_tag="ONT85_001037"
     CDS             complement(17051..17401)
                     /locus_tag="ONT85_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HCR2378030.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            complement(17558..19087)
                     /gene="ahpF"
                     /locus_tag="ONT85_001038"
     CDS             complement(17558..19087)
                     /gene="ahpF"
                     /locus_tag="ONT85_001038"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HCR2378031.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     RTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(19097..19660)
                     /gene="ahpC"
                     /locus_tag="ONT85_001039"
     CDS             complement(19097..19660)
                     /gene="ahpC"
                     /locus_tag="ONT85_001039"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HCR2378032.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(19804..20466)
                     /locus_tag="ONT85_001040"
     CDS             complement(19804..20466)
                     /locus_tag="ONT85_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR2378033.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            20650..22296
                     /locus_tag="ONT85_001041"
     CDS             20650..22296
                     /locus_tag="ONT85_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HCR2378034.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAVLLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPNMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDLQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            22287..23369
                     /gene="uxuA"
                     /locus_tag="ONT85_001042"
     CDS             22287..23369
                     /gene="uxuA"
                     /locus_tag="ONT85_001042"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HCR2378035.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFGLPGWEP
                     ERLAPIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLA
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(23477..25030)
                     /locus_tag="ONT85_001043"
                     /pseudo
     CDS             complement(23477..25030)
                     /locus_tag="ONT85_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            25178..26319
                     /locus_tag="ONT85_001044"
                     /pseudo
     CDS             25178..26319
                     /locus_tag="ONT85_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(26445..27524)
                     /locus_tag="ONT85_001045"
     CDS             complement(26445..27524)
                     /locus_tag="ONT85_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCR2378036.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVINFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(27627..28067)
                     /locus_tag="ONT85_001046"
     CDS             complement(27627..28067)
                     /locus_tag="ONT85_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCR2378037.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTPLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTAQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(28124..28534)
                     /locus_tag="ONT85_001047"
     CDS             complement(28124..28534)
                     /locus_tag="ONT85_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HCR2378038.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(28714..29148)
                     /locus_tag="ONT85_001048"
     CDS             complement(28714..29148)
                     /locus_tag="ONT85_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCR2378039.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVIFEERKQLFEKYM"
     gene            complement(29248..30096)
                     /locus_tag="ONT85_001049"
     CDS             complement(29248..30096)
                     /locus_tag="ONT85_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HCR2378040.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAALGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLVFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFIFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
     gene            30485..32251
                     /locus_tag="ONT85_001050"
     CDS             30485..32251
                     /locus_tag="ONT85_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HCR2378041.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETSRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVMDHLDYLTDLGINGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVNDREDKLYFTRKLEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            32353..33612
                     /locus_tag="ONT85_001051"
     CDS             32353..33612
                     /locus_tag="ONT85_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HCR2378042.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            33759..35060
                     /locus_tag="ONT85_001052"
     CDS             33759..35060
                     /locus_tag="ONT85_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCR2378043.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            35063..35914
                     /locus_tag="ONT85_001053"
     CDS             35063..35914
                     /locus_tag="ONT85_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCR2378044.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            35927..36742
                     /locus_tag="ONT85_001054"
     CDS             35927..36742
                     /locus_tag="ONT85_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HCR2378045.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            36893..38059
                     /locus_tag="ONT85_001055"
     CDS             36893..38059
                     /locus_tag="ONT85_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCR2378046.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            38289..38936
                     /locus_tag="ONT85_001056"
     CDS             38289..38936
                     /locus_tag="ONT85_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HCR2378047.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            39020..39964
                     /locus_tag="ONT85_001057"
     CDS             39020..39964
                     /locus_tag="ONT85_001057"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HCR2378048.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            40099..41550
                     /locus_tag="ONT85_001058"
     CDS             40099..41550
                     /locus_tag="ONT85_001058"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HCR2378049.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            41571..42407
                     /locus_tag="ONT85_001059"
     CDS             41571..42407
                     /locus_tag="ONT85_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HCR2378050.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            42407..43066
                     /locus_tag="ONT85_001060"
     CDS             42407..43066
                     /locus_tag="ONT85_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HCR2378051.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSMHQS
                     I"
     gene            43192..43881
                     /locus_tag="ONT85_001061"
     CDS             43192..43881
                     /locus_tag="ONT85_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2378052.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PTDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            43887..44843
                     /locus_tag="ONT85_001062"
     CDS             43887..44843
                     /locus_tag="ONT85_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HCR2378053.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHPIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            44887..45399
                     /locus_tag="ONT85_001063"
     CDS             44887..45399
                     /locus_tag="ONT85_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR2378054.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSSVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(45655..46203)
                     /locus_tag="ONT85_001064"
     CDS             complement(45655..46203)
                     /locus_tag="ONT85_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR2378055.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(46292..47473)
                     /locus_tag="ONT85_001065"
     CDS             complement(46292..47473)
                     /locus_tag="ONT85_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HCR2378056.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(47709..49217)
                     /locus_tag="ONT85_001066"
     CDS             complement(47709..49217)
                     /locus_tag="ONT85_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HCR2378057.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATSLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            49376..49567
                     /locus_tag="ONT85_001067"
     CDS             49376..49567
                     /locus_tag="ONT85_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2378058.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            49770..50687
                     /locus_tag="ONT85_001068"
     CDS             49770..50687
                     /locus_tag="ONT85_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="viral A-type inclusion protein"
                     /protein_id="HCR2378059.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            50680..51753
                     /locus_tag="ONT85_001069"
     CDS             50680..51753
                     /locus_tag="ONT85_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2378060.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            51901..52419
                     /locus_tag="ONT85_001070"
     CDS             51901..52419
                     /locus_tag="ONT85_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2378061.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            52400..52792
                     /locus_tag="ONT85_001071"
     CDS             52400..52792
                     /locus_tag="ONT85_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HCR2378062.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVFFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            52868..53803
                     /locus_tag="ONT85_001072"
     CDS             52868..53803
                     /locus_tag="ONT85_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HCR2378063.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            53936..54715
                     /locus_tag="ONT85_001073"
     CDS             53936..54715
                     /locus_tag="ONT85_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HCR2378064.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            54708..55025
                     /locus_tag="ONT85_001074"
     CDS             54708..55025
                     /locus_tag="ONT85_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HCR2378065.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            55269..56879
                     /locus_tag="ONT85_001075"
     CDS             55269..56879
                     /locus_tag="ONT85_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HCR2378066.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            57036..57884
                     /locus_tag="ONT85_001076"
     CDS             57036..57884
                     /locus_tag="ONT85_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HCR2378067.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
BASE COUNT        19269 a        10229 c        11305 g        17112 t
ORIGIN      
        1 aataaataga atcttgtaat aataactgag cataatcttt gcaattttta atcttgaata
       61 atatccttta agtatagcca gcaatcaaaa gtaaatagag aataagtaag caattgttat
      121 ttcagtatca gactggcaat aaaacgccgt tatgatccag caatgtttgg tagcgcttta
      181 tttactgatg ctatgctaac tgttcgctat ctatactgtg attgtcggta gagttattta
      241 cagggtaaaa ataagtttat aggaggtgga aaatgaataa ggggttaaaa ataatcggtg
      301 ttatcatgag tttactgcta ctttcagtat ttctagcgat tgcgttgatc aatgcgccgt
      361 atattatgcc tgaacaattg gaacgatttc gcttctttac tttaacgaat tactacatgc
      421 aacaatatat tttctgggca gctgtagtat ttgccgttct tgctattatc atcttgctag
      481 tcgttctttt ctatccaaag tcaagaggaa ctttcgttat gaaacgggaa gatggcaaac
      541 taacaatcga caaaaaagcg atcgaagggt tggtacgttc ccatttacac gaagaagagt
      601 tcattcattc acctaaagta cggatacgtt caacgaaaaa tcgaatccat atccatgtca
      661 acggagactt gaaacgaact tcttcattag tagggaaaac aggtgtattg atgcaagata
      721 tcgaagaaga agtaactaaa gtactcggaa caaaagaaac ggtcaaagtg gcagtcacat
      781 acagcggtta tgaagaacaa gaagatacaa gagactacaa gcactctcga gtggaatagg
      841 agggccaaat aatgaaagaa ctattttcag catacaagct ccctattatc tttgggggtc
      901 ttggactact gctagctgtt ttatggttaa cgattggatt tttcaagaca ttgttgatca
      961 taatcatgac agcaattggc gcaggaatcg gtttttattt gaaacagtca ggaatagttg
     1021 atcaattttt taaatcatag gaggaaacaa agatggaaaa caaaaacatg aatacaacaa
     1081 atggatcagt tttggcaaat gaaaaaggag aaaaccgcat gatgaatggt gaattgactt
     1141 atgaagacaa agtcatccaa aaaatcatcg gttatgcatt gaaagatgta gatggtctat
     1201 tgacattaga tggcggattt ttctcaaata tggctgaaaa attagtcaac acagataacg
     1261 taacagcagg tatcaataca gaagtaggta agaaacaagt agctgtggat ctagatgtga
     1321 tcgtagaata cggtaaagac atcgaagata tttacaacca aatcaaagaa ttgatcagta
     1381 ctgaagtaaa caatatgaca catttagatg ttatcgaagt gaatgtgaat gttgcagaca
     1441 tcaaaacaca agaagaatac caaaaagatc aagaaacagt acaagacaaa gtaacagaag
     1501 cagcaaaatc cacaggacaa tttgcttcac gccaaacaga taaagcaaaa tctgctgtag
     1561 gcaaaggtgc acaaaaagtc aaagaaaata acgaaccacg tgtacaataa gaagtaagaa
     1621 gtcagttagg aggaatttaa aatgggattt atttgggcat taatcgtcgg cggtatcatt
     1681 ggagcaattg caggagcaat cacgaataaa ggatcatcaa tggggatcat cgcaaacatc
     1741 attgctggtt tagtaggttc agcaattgga caagctttac taggaacttg gggaccaaca
     1801 attgcaggaa tggctatcat tccatcattg atcggtgcaa tcattttagt attagtcgtt
     1861 tcattctttg cacgtaaatt ataagcaaat tgaagaaaaa cttatgttga aatgaagaac
     1921 aaatttgaga accgtagcga cactcgctgc ggttcttttt tatatatttt atatgaagta
     1981 ttctttattc cttatgaaaa aatatcttta aaaataaaaa aagacgaaaa taaacggttt
     2041 tctataaaaa gaagaccgtt tattttttgt ctttatttgt gtgaaaaacg aaaaagagca
     2101 caacacatca agcttttatt aattgtctgt cttttataaa tgcaagtatt aattcaaaaa
     2161 aacagaagaa aaaaacaact acaccttctt ttatactcgt aatcgtagaa aggaggcgaa
     2221 aaaaatgaat acattaccta tcaatacagg agatacagca ttcatgattt tatgtacagc
     2281 aatggtttgc ctgatgactc ctggtctagc ttttttttac ggcggattag cacggaagaa
     2341 taatatacta acgatcatgt ctcaatcttt tctctccgtt gggataacaa cgattatgtg
     2401 ggtatttggt ggattcggat tggcctttgg acgggatatc ggcggtgtta ttggaaaccc
     2461 ggcagatttc tttttgatga gacatgttac aagcttcccc aacatgtttc atggtgcaac
     2521 gattccattt ttaatgttct ttctttacca actgatgttt atcgtgatta ccgttccttt
     2581 gatgacagga gcttttgcag ggagattgaa tttaaaaggc tatattatac ttgttatttt
     2641 ttggaatctg ctgatttatt tccctgtttg ccactggata tggggtggcg gatttttgga
     2701 tcagatggga tttagagatt tcgctggtgg agcagtcatc cacacgacag ctggattcgg
     2761 ctctttagca tgtatcctca cacttggtca aagaaagatt cctttaggag aaagaagtca
     2821 gcatagcagt cttatggcag ctgctatcgg tacaggactg ctttggtttg gctggttcgg
     2881 ttttaattcc ggtggtgcac ttcgagcaga tgaacaagca gtcaatgcat ttggttcaac
     2941 attcatcgct cttgcatttg cgatgattac ttggctgatc attgcaaaag taaaaggaaa
     3001 tggctttgat tttgtcgacg tactaacagg atccgttgct ggattggcag ccattacgcc
     3061 ttgtgccggt tatgtagaag caaaaagtgc catgctgatt gggatcattg cgggtatcgt
     3121 ctgtcatgct gctgttgatt ttcgtaaaaa gaaacaatgg gatgatgcac ttgatgtgtg
     3181 gggtgttcat ggcatgggcg gattcacagg tacgattctg atagggatct ttgcatctaa
     3241 cagccattta ctaacgaatc caagcagctg gtacttctta gggattcaag tgattggtgt
     3301 catcattact gccgtgtatg cctatgtgct gacaacagtg attttaaaat cggcgatgca
     3361 ctttacaacg atcactacta caaaagaaga acaagaagaa ggattggact ttttcttctt
     3421 tggcaacaaa ctcaattgaa acaaattcaa atagaaaaga ggaagattca tgcaacaaag
     3481 caaaacgaaa attcctgcaa aaaaattaac gttctttggc tttttggcta tgaccatctc
     3541 aatggtcgtt tcattatatg aatatcccac attcgcgacc tcaggcttca gcttagtatt
     3601 tttcttgtta ttaggcggtt tgctatggtt tatccctgtg gcattgtgcg cagcagagat
     3661 ggcaacggtg aaaggatggg aaaaaggcgg tgtctatact tgggtcagtc gcacattggg
     3721 aaaacgattc ggttttgccg caatcttttt ccaatggttt gagataacag ttggctactt
     3781 gaccatgctg tatttcttaa ctggtgcatt atcctatgca acgggtattg gtgctataca
     3841 aaacaataaa tatctgaaat tggcaatcct attgattatt ttctgggcta ttttactctc
     3901 acagttaaaa ggaaccaaat acacttcatt gattgctcgg atcggttttg tagctggtat
     3961 cttgttgcca gcattagtct tgtttggctt agggatccat tatgtcgctt caggcgctcc
     4021 actacaaacg atactgtcta tgaaaacatt gattcctgat ttttcgaaat tgcctacgct
     4081 ggttgttttt gtttccttta ttttagcata tatgggcgta gaaacatctg ccagtcatgc
     4141 aaatgaaatg gaaaacccta aaaaggatta tccattagca atgtttgtat tggttattct
     4201 ggcaatcatt ttagatactt ttggcggatt gaccgtcgct actacagtac ctcaacatgg
     4261 tttgtctctt aatacagggg tgattcaatc gttagaattc ctattaagac acattgatcc
     4321 aaatcttgca tggattgcga aaattcttgc gattcttgtt tgtttaggtg tcatcggaga
     4381 aattgcttct tgggtaacct ctccttcaaa agcacttcac gtagcagcag aagaaggatt
     4441 acttcctgaa tattttgcaa aagaaaacac acacggtgtt cctgctcatt tgatgatcgc
     4501 aaatgggatc gttgcaacaa tctgggcagc cgtctttaca ttaagcggcg gggggaataa
     4561 tctttcattt cttgcagcga tgtcattgac tgttgtaatc tacctgatgt tgtactttat
     4621 gtttttcgcg ggctatttga agctggtttt taaagatgac agtcttgaaa gagcctatca
     4681 ggttccaggc ggaaaagttg taaaagtaat cgttgcttgt ttaggtcttt tgacttcact
     4741 ttttgctttt tgtatctcat ttgttccacc tgctagtatc gcagctggac agcatgggat
     4801 ttatgaaacg attctgattg tttcttatct tatcgtctta gttattccat ttacgatcta
     4861 cgcatgccgt cataaattcc agcatgcaga aaaataaaaa atgaaagctg gttattcaaa
     4921 tggaagtaaa agaattagaa caattgattg gaaaaaattt ccactattat caaacaggac
     4981 aagtggcgga ttacattcca gcattagctg aagtggatcc caaacaatta ggtatggcga
     5041 tttatgattt ggatacaaaa caactgattg agggaggaga cgccgatgtg cgctttgcca
     5101 tcgaaagcat gtcaaaagtt cccgtattgt tattagcaat caaagacaac ggaatagaaa
     5161 aagtctttca aacaatcaac acggaaccaa ccggttttgc cttcaattct atcatgaata
     5221 tggaaatcaa tcatcgcaaa catccaatga atccttttgt taatgcagga gcgattgcaa
     5281 caacctcgtt gattgccggt aaagatgcag atgaaaaatt tgagcgaatc ttgtcattta
     5341 tgaaagagat ctgtgatgat cctaatatca cattgaatga agaaatctat cattctgaat
     5401 caagaacagg tgatatcaat cgctctcttg cttactatat gaaaggtaat caaatgatcg
     5461 aaggagacgt accggagata ctcgatgctt atttcagaca atgctccctc aatgtcaccg
     5521 ctacaggcat agcaaaatta gctgctgtgc tggcaaataa aggaattgct ccttggaatg
     5581 gcaaacaaat cattactgaa gaaagtgcta caatcgtcaa atcaattatg acaactgctg
     5641 gtttgtatga tgaatcagga gagttttccg tccatgttgg tgtacctgca aaaagcggtg
     5701 taggcggcgg gttgatggca gctgtgccaa atcgttacgg cataggtgtc ttcagcccgg
     5761 cactagatcc atttggtaac agcgcagctg gtatccaatt gttgaaagat gtagtcaaag
     5821 aactagacgc tgacatcttt gaataaccat tcttgaaaga ggctggggta aaagtccaaa
     5881 cggctcaaag ctacctacaa tgaggctgaa attgtgagct tgtttgactt ttgtcccagt
     5941 ttcttttttt attggttttt gaaagggctt ttctaaataa aaaggaaaca aaaatacgat
     6001 ttacagatat gattaaataa aatcataaga aaaactggag gcgtgtatca ggaatgctgg
     6061 aaaattacat cgagcgaaat atatttagaa aagtgtatct gtgtgaacaa ttatttgaat
     6121 tccaagaaat agacatcgaa caaacagcta tttctctaag ggtgactaca ccaacgatcc
     6181 ttcacgatct agaaagtcta gccgagtgtc tagaatattg tataaaagaa caagtaagag
     6241 agaagcataa gtataaactc gtattcaaac atggtattgc tctttctgag ctcacacagt
     6301 ttctgtacgg tcaatcttat tttttaaagt ttttaagtta ttattttaat ggtatattca
     6361 cgagtacaga actggctgat cttgaattta tttctttgag caaagtatat acaatcaaaa
     6421 aaatcgtatt ggattttttt aaagaaaatg attatttgaa aaataaagaa atcattatcc
     6481 cagaatttga ctcgcggaac atcttgctag ctttagtacg ttatgtcaat tgggaaggct
     6541 atgaaaataa aaatccgtat gtagaagaag cttgtaacga gttgattgat tatgtagaga
     6601 ctcatttttt taaaagaaga tattcggaag atgaaaagtc ctttatcata cgaggaatag
     6661 agatcgcaat cggaagaaag gcatttcccc ttggactgaa agaagaagat aagaaaagcg
     6721 cagaaacgaa gccgttattt caattagtct gtcaaggatt ggaagagcaa aaaggaattc
     6781 tagacttaca agaagaagat gtctattata ttttttcttt gttcaacagt agaaattata
     6841 ccaatgagaa catggagctg ttaagaaaag atttcgaagt tgtttataaa aattttgttc
     6901 tcaatgacct atcactgaat gaattgattt tacagattat tagtatcgct gaaatcgacg
     6961 atgcagaaaa ttttattttc aagcaatcct ttttacaatt tgtccgtacg ttgtgggcag
     7021 atggacaagt atttttaccg gaaaggatct atcttctatc agaaaaagaa caacttctgt
     7081 acggaaaaat tgtagaaata ttggaagagt ggaaaaagaa aaatcaaatc caactaagat
     7141 ggaatgaaaa tttcattcga aaattcgtca aaagtctttc tttgatcaac agtgaacaag
     7201 cggggagaca ggaaatagaa atttttgttg tttcagaaag ttcagtcaaa caattttttt
     7261 atcgaaccca atttcatttg tatgcggaag aaggcatcgc tgaaataaat cctattttat
     7321 ttcgttcatt agaagaattg cctgatgaat gtctatgtgc gaaaaaaaga gtggttttgt
     7381 gcgatgctgc tagctatcaa aatggattag aaactgaaaa gacaaagatc tatccaatct
     7441 cattaaaagg tgcaaacatc catttgtatc atattgctca ggaacttcac ctattaaaaa
     7501 aacatatttc ttgatgagac cttaattagg agaccttcta caaagctttt taattgtctg
     7561 tagaaggttt ttatttgagt cctgattcgt gcgaagatct acttatataa caagctttct
     7621 ctgaaatttt tttaaaatgc tattgactta gagtcaactc taagtagtag aatagataaa
     7681 gtgtaacaaa tctattttca ttaaaagaag atagtacgga ggagagaatg tattatgagt
     7741 acagtattac atgtattgga tgcaatcaat agccccgcag atgtcaaaaa attatctgtt
     7801 gaggaaatga aacagttggc agaagagatg cgccaaaaaa tcttgcaaaa ggacagcgat
     7861 gtaggtgggc acgttggtcc gaatctaggt gtagtagaat taacgattgc gtttcattat
     7921 gtatttgatt caccaaagga taaaatcgtt tgggatgttt cccatcagtc ttatggtcat
     7981 aagcttttaa ctggtcgaaa aaatggtttt gaaaaaggac attaccgtga agtgagcgga
     8041 tattccaacc aacatgaaag taaacatgac tattttacag tggggcacac atctacatct
     8101 atcagcttag caacaggttt agctaaagcg agagatctga agaaagaatc aggaaatgtg
     8161 gtagcattta tcggtgacgg ctccctaagt ggtggcttag cttttgaagg attgaacaat
     8221 ggtggtgttt tacaatccaa tctaatcgta ttagtaaacg ataatgagat gtctattgat
     8281 gagaatcatg gcagtttata taaaaactta gctgagcttc gtcaaaccaa cggtaaaagc
     8341 agcaataacc tatttaaagc aatgggattt gattatcgtt acgtggataa tggaaacgat
     8401 ctggaaacgg tgatccgttt atttgaagaa gtaaaagata ttgatcatcc gattgtactt
     8461 cacgtccata cggaaaaagg tcgcggttat cagccggcaa tcgataataa aatgaaatat
     8521 cattggcatg taccatttga tctgtccacc ggggaggcaa agcaagtaac ttctggagaa
     8581 acatttaata tgatcatcat ggattatttg gataagaaaa tgcaaacaag tacagctcct
     8641 attgccgcag tcaatgcagc gataccaggc gcctttggac tgaaaacatt ccaagaaaaa
     8701 catccagatc attattttga cgtggggatc gctgaacagc actctatcac tttcgcttct
     8761 gctttggctt ctcagggagt acgtccagtc gtcttccaat caagtacttt cttgcaacgg
     8821 gcatatgatc aattatcaca tgatttagcg atcaacgaga atccagtgat tattttagtc
     8881 ggtggcggaa cgatctcagc tagtgaccga acacaccaag gaacatttga cattccttac
     8941 ttatcaagta tcccgaacat caaatatctt gctccagcaa caaaagagga tatgatctct
     9001 atgctagatt gggcgttcga acaaacagcg cagcctgtag caatccgaat tcctacacat
     9061 ggtgtagaac atggtacagc tatcgcaaat gattatagta ttccagccta tcaaacagta
     9121 aataatggag gaaaagtagc aattttagct ttaggcggtt ttcttgaatt aggaaaagaa
     9181 acagtaaaag cttttagaaa aaaaggaatc gaagcaacgt tgatcaatcc aggaacgatc
     9241 acagaaaatg acgtagaaac attagataaa ttaaaagaaa cacacgaatt agtcatcaca
     9301 ttagaagatg gtagtttgtc aggaggattt ggcgaaaata tcagtcgtta ttatggtgat
     9361 tcttcaatga acgtattaaa ttttggtgca gagaaagaat tcacggatag cgttcctgta
     9421 gggcagcttt atgaacgtta tcatttaaca ccaaaacaaa tcgttgaaga tagtctgcgt
     9481 gtcttgaata ttcaataatt cattatgctc accatccaga taattgttta tactagtatc
     9541 tggatggttt tttcattata aaaagcaaag gggtggatgt tatgtatcag caagtagaag
     9601 aatatgtcaa aaagttccaa gaaaaatatc ctagttatca atcagaaaaa ttaggtttct
     9661 tttcatttgg ggatacagaa aaaatggcag acgagttgag cgaactagtt gtaaaaaaga
     9721 taaagactgg tacatcaagt ggttacgatc tgtataaaca agagaatgag cctttaccaa
     9781 aagttggtca attagacgtg gtcctgaatg gaaaaaatga accagtttgt atcatcaaaa
     9841 atataaatgt gaccatcttg ccttttagtg aagtcacgaa agagcatgct tggaaagaag
     9901 gagaagggga tcggacatta gcttattggc ggaaagtcca tgctgagttt tttaaacatg
     9961 cttataaaga agcgttgaac atagaattta cagaagagaa gctggtcgta tacgaagaat
    10021 ttgaagtaat tttcttttag taagaaaggt ctgctgaaag aaatagctca ttattgtctc
    10081 ctgaatagaa aagggttaca tttttatatg aagctgtatt tttatataac attactggag
    10141 gagatattgt ggatacactg gaacaaatca aaggcggagt cgtcgtttct tgtcaagctt
    10201 tggaaaacga gccgctacac agttctttta ttatggggag aatggctttg gctgcaaaag
    10261 aaggcggagc agttggtatt cgagcaaata cgacagaaga tatcaatgaa atcaagcgaa
    10321 tcgttgattt accggtaata gggattatca aaagagatta cgatgggtca gaagtgttta
    10381 ttactgcgac agaaaaagaa gtggaagaac ttttggagac gcaagcagaa atcattgcac
    10441 tggatgcaac ttctcgtaaa cgtccgacag aaaaaacaac tgctgaactt gtccaaatga
    10501 ttcacgaaca cggtcggtta gctatggcag atatttcgac ttttgaggaa gcgcttcaag
    10561 cgcaagaaga tggttttgat cttttgtcta cgactttagc aggctataca gaatattcac
    10621 gaaagacgca gacacctgat tttgatttat tgaatcaaat catagaacaa gtcgatatcc
    10681 cagtaattat ggaaggacat actgactctc ctgaacaagt agagaaagca tatgaattag
    10741 gtgctttttc agtcgtagtt ggatctatta tcacccgtcc gcaattgatc actaaaagat
    10801 atgtcgaagc cgcaagtaaa gcaaataact aaaaaacacg cacggcacag attcttgaca
    10861 aaaaagaaaa attgatagat agtttaaagc agattctaag aagatgaaaa aagcattact
    10921 tttcattttg tcttcaagat ctgcttttta acgaatttta tagaaagaag gcgaaaccat
    10981 gtttgaacgg agtccttcag aattgacaga acgtgaaaat tacaagttgc tgattggttc
    11041 agtcatccct agaccagtag cagtagtaac gactaaatca gcaaaagata tagtgaatat
    11101 cgcacccttc agttacttca atatcgtcag ttcgaatcga cccatcttat ccttggcgat
    11161 ccagagaaaa caagatgcag taaaagacac agcaaagaat attcttgaga caagagaagc
    11221 tgttatccat atccttgatg aggacaacgt agaagaagta aataaaacag cagccaatct
    11281 gcctaatgag gaaagcgaac tgtacctgac tgacttgact ttaactgatt cagtaatcat
    11341 ttcagtacca gcattgacag aagcaaaagt tcgttttgaa acatccttat atgaacatgt
    11401 cgagatacgg aataaaaata aagtaacagc tgatttattt ttgctagaag tacagaaata
    11461 tcatttaaat gaaacagtct atgatgataa gacaggaagg atcgatccaa gaaaattgaa
    11521 tgctgtaagc cgtttggcag gaaatgatta tgcagcaata ggagaaattt tcacgattgc
    11581 acgtcccgaa taaaaaggag cactagttat gttaaaaata agctataaac catcgacaga
    11641 ttcaaaagaa atgaaaaaag aatatgaaac agtgaatgat tttctgcaag ggcagtactt
    11701 agaagttccg ccattgcaag accattttgt cgttactact gttactttag atggtaagga
    11761 aatagaaatg ccagatcaaa cgattagcgg attgtttaat tactttaaca aataaatatt
    11821 tattccgaaa agtgaaaaaa catataaaaa aacacataaa actaaaaaat tatcaaagaa
    11881 tcctggtgca actgaaagaa tttgcaacgg gattctttca acttatttgt tatttttttc
    11941 taaaaaatat taaagattgt taaatttcac tatttcattt caaattccat tgcctaatca
    12001 ttaggaaaag tctatcatga actcgtaaat agaatatagt aataaccttg ttaggtgagg
    12061 ctcctataca aacataggct gttgcccaga aacgtcgaaa gtcgccaatg ggtaaaacag
    12121 aaactgttgg attaaggctt tttccaatag ctaagaatgt tttctttacg ttgtatagtg
    12181 ctaaaactcg gacgatgaga tgatgctagg tcagaattct tgttcgaaaa ggtttctgac
    12241 cttttcttta tatttttgtg actgacttat ttgatgagac aaactcgctc ctcacctgac
    12301 aagtggattg ctgtgtgagg aggagatgcg gtgaaaaaag atcgattggt ggaggtcgct
    12361 cggattcact tctggttgca caatacaacc agaaaacaca acaagcaaaa ctgatctcta
    12421 tcatgagaga ttcatatgtc gatattccag gttacggaat gaataagata aatgcagctt
    12481 actcttatgg gggagttgat ttattaaatc aaacattaaa ggaaaatttc aaatttgaag
    12541 ctccctatta tgcaagtatc acatttcaag attttatcga ttgcgttaat gaactgtttc
    12601 ctggtggagt caagatcgat gcagaaaaat ctctagattt agatggcgta tatataaaca
    12661 aaggagagca agtgatggat ggtaatactt tactccagta tgctcgattt cgggaagaca
    12721 aagaaggaga cttcgggagg atcagaaggc agcagcaagt gatcaaagct atctctcagc
    12781 agctgaaaga cgtaacttca atattcaagc tacccaaagc ggttgggaaa ttacttggta
    12841 gtatccaaac gaaccttcca gaaagtgtgc ttctggattg tggtatggat ttcctcaaag
    12901 acatatgtta gaataaacag gtataaatag tgaaataaag gaatgacaag caagagaagg
    12961 aggaaaaaat gaaaagaagt gacaagcacg gcaaaaatcg aacaggcgct tatattgccg
    13021 gcgcagtgat cttaatagca gctgcgggtg gcggttattt ttactaccag cactatcaag
    13081 aaacccaagc agtagaagct ggagaaaaga cagttgagca atttgtccaa gctttgaaca
    13141 agggagatta taacaaagct gcagaaatga catcaaaaaa ggcggcaaat aaaagtgcat
    13201 tatctgaaaa agagatctta gataaatacc agaatatata cggtgctgct gatgtcaaag
    13261 gacttcagat atcgaatcta aaagtagata aaaaagacga ttctacttac agtttttcat
    13321 ataaagcaaa gatgaatacc tcattaggtg aattgaaaga tctttcttat aaaggaacat
    13381 tagacagaaa tgatggtcaa accacgatca actggcaacc caacttggtt tttccagaaa
    13441 tggaaggaaa tgacaaagta agcctgacca cacaagaagc agcaagaggg aatattatag
    13501 atcgaaatgg tgaaccatta gcaacaacag gcaaactaaa acaattagga gtcgttccaa
    13561 gtaaacttgg agatggaggc gaaaaaacag ccaatatcaa agcgattgct tcctccttcg
    13621 acttaacaga agatgctatc aatcaggcga tttcacaaag ctgggtacaa cccgattact
    13681 ttgtcccatt gaaaatcatt gatggagcaa cgccagaact tccagctgga gctaccattc
    13741 aagaagtaga cggcagatat tatcctttgg gtgaagcagc tgctcagctg attggttacg
    13801 tgggagatat cacagcagaa gatattgata agaatccaga attaagcagt aatggaaaaa
    13861 tcggtcgctc aggtttggag atggcttttg ataaggatct tcgtgggaca acaggtggga
    13921 aattaagcat tacagatgca gacggtgtag agaaaaaagt tctgatcgaa catgaagttc
    13981 aaaatggcaa agatatcaaa ttgacaatcg atgcgaaggc acaaaaaata gcttttgaca
    14041 gtctaggagg aaaagctgca tcaactgttg cgacaacgcc aaaaaccggt gatcttcttg
    14101 cgcttgctag ctctccaagc tatgatccaa acaaaatgac aaacgggatc tcacaagaag
    14161 attacaaagc ttatgaagaa aatcctgaac atccattcat cagccgattt gcgacaggtt
    14221 atgctcctgg atctacgttt aaaatgatta cagcagcaat cggtctcgac aacggcacta
    14281 tcgatccaaa tgaagtgttg acgatcaacg ggcttaaatg gcaaaaagac agttcttggg
    14341 gatcttttaa aacaacgcgt gtcagtagtg atgtatcaca agtagactta aaaactgctt
    14401 tgatctattc cgataatata tatgcggcac aagaaacgtt gaaaatgggt gagaaaaaat
    14461 ttcgtacagg cttagataaa ttcatttttg gtgaagacct tgatttgcca attagtatga
    14521 atccagcaca aatttctaat gaagatagct ttaactcaga tatcttgcta gctgatactg
    14581 gatatggaca gggcgaactt ctaattactc ctatccagca agcagcaatg tattctgttt
    14641 ttaccaacaa tggcacactt gtctatccta aattgattgc agataaagag acaaaagata
    14701 agaagaatgt catcagcgaa acagcagtac aaacgatcgt gccagatctg agagaagttg
    14761 tgcaagatgt aaatggtaca gcacattctc tttctgcttt agggattccg ttggcagcga
    14821 aaactggtac agcggaaatc aaagaaaaac aggatgtaaa ggggcaagag aacagtttct
    14881 tgtttgcttt caatcttgat aaccaaggtt atatgatggt cagcatgttg gaaaataaag
    14941 aagatgatga ttcagcaacc aaacgagcat cggaactatt gcaatacctc aaccaaaatt
    15001 atcaataaaa aattgctttt taagtacagt aaaaggactg tggcgttatc gtcacagtcc
    15061 ttttttcaaa taaacggttt tcacgatgtc tctttcacaa cttctttttg atttactcac
    15121 acttcataaa ctttttttgt aaaatatttg agaacaagtg gagaaataag tgtacttaat
    15181 aaaatcacga taactaaagg tgaatagtac tgtggttcga tcaattgact ttgctgtcca
    15241 atttgtaaaa tgatcaaagc catttctcca cgcgaaatca taccagcacc gaccatcaat
    15301 gaactatgtt gagaaaaacc ggcaatctga gcaccaaaat aacctccaag caatttggtc
    15361 aaaatcgcta caagcgtcaa tatagaaata aagagtagtt gttcactaaa gtcagaaaaa
    15421 tcaacttcta atcctacact gacaaaaaat actggtataa atactgcata tcctaaagcc
    15481 tctacattgt ggtatacttc ttctttaacc tttgtctgac tgacagctat cccagcaaaa
    15541 aaagcaccaa tgacagagct aagcccaaca agatccgcta cgtaagacat tcctaaacaa
    15601 ataactagcg atacgatgat aatagaagaa tttgcgtaca ctttttctgc aaatgaaagt
    15661 aggaaaggcg caatccattt gactaataag aaaataaaaa caaaataaat tagctgttca
    15721 atcaaaacaa gcggtaaaga agcattgcta gaagcttccc ccgttagaaa agataggctg
    15781 aaacttagaa ctaaaacgac taaaatgtca tctaccacag aagcacccaa aattgttgat
    15841 ccttcttttg tattcactac gtttagttct tttaatactt ctactgaaat actgacagaa
    15901 gttgccgcaa gaataatccc aaagaaaatc gcttcatttc ccgaaacttg gaatgctttg
    15961 cctgtcaacc agccaaaaac aaccgggaag atgatcccaa gcaaagcaac gaacatacca
    16021 ggtcggaaat attttttcaa aagagataaa tcactttcca gaccagctaa aaacattaat
    16081 aagatcacac caatctccga aaagtcatgg actagaatat caggtttgac ccaattcaat
    16141 ccagcttggc ctagcaaaac accaacaagt aattgtccaa tcactgctgg gatacctaat
    16201 ctccgcgaga tatgtgagcc aatcgttgtt gaaatcaata tcaggcataa aataccaata
    16261 aattccattc tcatcattcc tttgtataaa atatagcccg aacttagcat aacatacatt
    16321 attgaaaaaa taggaaaata tactcacaaa aaaaccaaca atccaaaaga tttttttgga
    16381 ttgttggttt ttatacggtc tattttaata actaaaaatg attaacgtat agtcattttt
    16441 cttattctga tgaaattgat aatctcctgg atgcccatga aaatcaaaat aaagccgaat
    16501 atttggaata gaagcatcaa acttttgaat ggattgaaca ataagacgaa tccgccaatc
    16561 aacaacagaa tactactata aacactccaa cttttagctt ttgtattcat gctgaagaac
    16621 aactggaaca agctgttgat gataacagct aatccaagaa gaatcggcag gatagaaacg
    16681 attgcaggtg caaaagcata gacgatcaga gctaagatca ggaaaaaaag accactgaac
    16741 agaccgatac tccaagaacc tgttttcctt ttaaaacggt aagcctcaaa aagattcaac
    16801 aacccaagca aaacaagata ggcggtgatc agataaccga taaaatgaaa aacagcagaa
    16861 gggtttagca caatcgcaat cccagaaata atataaacag cagctcttaa taaggcgtat
    16921 ttttgaatat tttgtttaat cgtctcaaac atataagaaa acctcctttg acttatttta
    16981 acgtgaacta gtcattttac caaattaaat gttacaaaat gagcgaagga gaagaatatc
    17041 tctggaaaaa tcaacttctg aggatctttt ccattttttt gccttttgct agctcatcga
    17101 tcattttatc taaataacga atttctttca tcaagttatc ctcgatgtct tctactcgta
    17161 caccacaaac aacacctgta atcaaggaac gtgaaggatt ggggacaggg gcttccgcaa
    17221 aaaattgttc gaaagtcact tgtttttgta gttgatattc tagttctgtt tgggaataac
    17281 ccgtcagcca acaaatgatt tcatctactt cctcttttat acgatctttt ctctccgctt
    17341 ttgctacata cataggataa acgcttgcaa aacttgtcgt aaaaatacga ttttgcgcca
    17401 taatcagtca cctttctttt tttcaaatta tttactactt atagtatata agaaaaagag
    17461 aagaaaaagc gattattcaa aaaaacaaaa aggattgtga caaaattctt gtcaccatcc
    17521 ttttttccgc tctctgactt tttacaatct tcatagctca agtgcgcatc agataatcga
    17581 atgctcctaa agctgcagta gctcctgaac ccattgaaac aatgatttgt ttgtatggac
    17641 tatctgtaca atcaccagct gcgaaaacgc cggggatatt cgttgcgcca tttttctcgg
    17701 tcactatttc atttcttgga ttcatttcga tcgttctatc tagccaatcc gtattaggga
    17761 gtaagccgac tagaatgaat actcctgaaa cagctagtgt atgcatctcc atcgtacggc
    17821 gatcgatata ggagagagat tctacctgat tcgtaccgct gatttctttc gtcgctgcat
    17881 gtttgataac agtgacattc gcaagagaga ctagtttatc ctgtaagatt tgatctgctt
    17941 ttaattctgg taagaattct agaacatata catgttttgc taacccagcc aaatcgattg
    18001 ctgcttcaac accagaatta ccaccaccga taacagcaat ctcttttcct ttaaataaag
    18061 ggccgtcaca gtgtgggcaa taagcaattc ctttattttt aaactctttt tcgcctggta
    18121 cattgataga acgccagtgc gcacctgtcg cgatgatggc ggtttttgct ttaagtgata
    18181 taccgttagc taagccaata ttaataaaat gttgctcttt atttaaggaa acggcctgtt
    18241 gattcatcat aatgtcgata gggtaactac gcacatgact ttctacttgc tgcatcaatt
    18301 ttggaccttc tgtgtaagag gtaccaatca tattttcaat ccctagtgtt tcaacgactt
    18361 gtccgccgaa agaatcagcg acaagtcctg tacggattcc tttacgtgct gcatagatag
    18421 ctgcactact agctgctggt ccaccgccaa tcactaatac atcataaggt tctttttcaa
    18481 acaactcttc ttcaatcaaa gggccagtga ttttttcaac aatttgttcg atcgtcatgc
    18541 gtccgctact aaattcttct ccatttaaaa acacagttgg cactgccatg atttttttat
    18601 catcgatttc tttttggaac atcccacctt caatcatcgt atgagtaata tttggattta
    18661 atacagctaa tatattcaat gcctgtacaa cgtcggggca attatgacaa gtcaagctaa
    18721 cataactgtc aaaatgcaat gtttgtgtta tttttttgat tttaagactc aaagattcac
    18781 tgattttcgg tgcccgtcca ctaatttgta acatagccag taaaaaagag gaaaattcat
    18841 gaccgagggg gataccagca aaaacgattc cactttcttt gccaactgga ttgatagaaa
    18901 aactaggtgt gcgcatcgaa tctctttttt cccaagagat tttaggagac atttctgcta
    18961 tttccttcgt aaattcagct agtttttgac tattttcact attatccaaa ctaacagaaa
    19021 aaacaatagg agattcaatc agtgctaaat attgttcgag attttgttta gtttctttgt
    19081 ctaacatatc caccacctaa atctttccaa caagatcaaa acttggtttc aatgtttcgc
    19141 cactttcttt ccattttgcc ggacaaactt cacctgggtg ggtacgtaca tattgagcag
    19201 cgcgaatctt atcaatcagt gcactagcgt cacgaccgat tccatctgca ttgatttcca
    19261 tagcttgaac gattccgtct ggatcaataa taaatgttcc tcgctgagct aatccttgct
    19321 cttcatccaa aacatcaaaa atacgagaaa tctgatgaga cggatcaccg atcatcacgt
    19381 attcgatttt accgatagca tctgatgtat catgccatgc tttgtgagta aagtgggtat
    19441 cggtagagca agaaaagact tcaacaccta aagatttcaa tgtactgtat tgttcttgta
    19501 agtcttctaa ttcagtagga caaacaaaag taaaatcagc tggatagaag caaagaatac
    19561 tccagttccc cataagatct ttttcactga cttctaaaaa ttctccttga tgataagcat
    19621 gtgcattaaa ttcttcgatt tttttcccaa tcaatgacat agtaaaacct ccaataataa
    19681 ttatttattt ataattattg taatttagtt ggagttgcaa agtcaatgga tattttgtta
    19741 tttaataaag attactcaaa aacgaaggtt aattataaaa atagaagagg tcgcaagaaa
    19801 atgctactgc tggaaatact cgggataacg ttgagtaaca gtttcctgtt ctttcagcat
    19861 catgtgtaag tgcaatcccg tttgaaatcg agcttcttct ggttttttat caactaaagc
    19921 cttgagtaaa tcataatgtt gttccatgat cgtttcccat ggaagcccct gaatggtcac
    19981 tcgtaaccag cggaaacgtt ctaaatgggt attatggtca tctaaccagt tccaaacttc
    20041 ttttcttcct gcaatttcga aacaagcttc atgaaataaa ttatctgcac ggaaaaaagc
    20101 gcgttcgtca tttattttga ctgctttttc ttgttcttct aaaatcgttt ctagcactat
    20161 tcgtgtttgt gtgtccaatt tggaacaaca ttcaatcatg atttgctctt ccaactgttc
    20221 tcggacaaag cgagcatttt ctgcagaaac aagattaatt ttggaaacat acgtaccact
    20281 ttgaggtatc gtatacacga gttcttgctg acgtagttga atcaatgctt cgcgaatggg
    20341 agtccggccg atagcaagat gttcctccag tgccttttca gatattttcc ttccaggttc
    20401 taagtcagca tagataattt gtttacgaat cgcttgataa gcttgtgatt gcaagctgct
    20461 tttcatcttt attcctccaa aatcatttct atgaacgtag tataacagaa gaggtctgaa
    20521 aaacaatctg gtatgtcaca ttaactagta aatttgtata aatagtaaaa ttttggttga
    20581 catagcgatt tttattgcat aaaattcact tgaatacaaa aactaatatg ttagtttagg
    20641 aggggaaaaa tgattgattt aaacaactgg aggaacttta tccctgaatt atctcaaaaa
    20701 gaagtagaaa tacctcattt tgaccgtgca tcattaagag aaaatggaca aaaaaatcct
    20761 gtctggatcc atttcggtgg cggaaatctt tatcgaggat ttcatgcaga aatcgctcaa
    20821 cgtttagcgg atctaggcga attgtccact ggtgtcattg tttgtgaaac attcgatgaa
    20881 gaaataattg aaagggctta ccaagatttt gatgatttga ttcttgaagt ggtcatgcca
    20941 agcagtggag agttcgtcaa acgtatatta gctgcgacag ctgacagtct gtattgtcat
    21001 ccagattgtt ctgaacactt tgataagatg attcgacact ttgaaaatcc tagtttacaa
    21061 ttagttactt ttgctattac tgaaaaaggt tatcgattga aagatacaaa taataaatat
    21121 ataggaccga ttgcagtcga tataaaaaat ggtccaaaga agccatcaca tacaatgagt
    21181 attcttgctg tgctacttta taaacgtttt ttatctggag cttggccgat tgcacttgtc
    21241 tctacagata atttttcaca aaacggtagt caattacgta cttctttgct aacgatcgca
    21301 aaagaatggc aaaaggaagg ttatgtacaa aatacattta tccaatactt atcgaatgaa
    21361 gaaattgttg cgtttccatg gacgatgatt gatcgtatca ccccgaatcc ttccttgtca
    21421 gttagcaaaa tgttggcaaa agagggcttt tcaaatatgt cgattttcca aaccgcaaaa
    21481 ggaaataata ttgctccatt tgtcaataca gaggacatcc attatttaat catggaagat
    21541 cattttccca atggacgtcc gaaattcgaa aaagctgggg tattcttgac tgatcgtgca
    21601 acggtagata aagcagatac gatgaaggtg acgacttgcc ttaatccttt gcatacgtct
    21661 ttagctatta tgggttgttt actggggtat aggagcatag ctgaggagat gaaagatcca
    21721 gatttatcgc tgctagtttc tgaaatcggg tacaatgaag gcctcccagt tgtggagtct
    21781 ccaggtatca ttgatccaaa gatctttctg gaagaagtaa ttactgtgcg cttttctaat
    21841 ccaaatattc ctaatatgcc tcagcgaatc gccacggata cctctcaaaa ggtggcgatt
    21901 cgttatggtg agacgattaa aaaatatatg gaaatagaag ttaaagaccc gcagacactt
    21961 gtattcatac cattgactct cgctgcttgg cttcgatacc ttattggcat tgatgatcaa
    22021 ggaaaaccat tcatcccgag tccagaccca ctattgacag accttcagca taagctttcc
    22081 gatattcaac tgggtacgca agatacatca catattcatg agaaaatggc tcctattttg
    22141 tcaaataaag aaatttttgg tgtagatctt taccaagcag agcttggcag aaaaatagaa
    22201 gaaatgttca tcaagatggt agcaggacca aatgccgtac gaaaaacatt acaggattac
    22261 ttacaagaac atggaggaaa tttatcatgg aaatgacttt tagatggtac ggatcacagg
    22321 aagaaaagat taccttagat gaaatcagac agattcctgc ggtcaaaggg attgtaggca
    22381 cattgatgga tataccagta ggagaggtat ggcctaggga gaggatcaaa gaactaaaag
    22441 aagaaattga gtctcgtggg ttaacactaa aagtaattga aagcgtcaat gtgcatgatg
    22501 ccattaaaat tggattacct tcccgtgagc agtatatcga aaactataag cagacaatcc
    22561 gaaacttggc agaatatgga atagaagtca tctgctataa ttttatgcct gtatttgact
    22621 gggtgaaatc tgaccttgac taccgcttag aagacggctc atccacgtta gcttttatat
    22681 ctgatgatat tccagatgat ccaagagaaa tcgtcgcgcg catcgaacat tccagcaatg
    22741 aatttggatt gccgggatgg gaacctgagc gcttagctcc tatcaaatcc ttgcttgaag
    22801 cctatgcatc agtagacgaa gaaaaacttc gagaaaattt tgcctatttt ctccaatcaa
    22861 tcatcccaac atgtgaagag gtaggggtca aaatggctgt acatccagat gatccgccat
    22921 actcgatttt tggattagct cgaatcgtaa agaatcgaga agatttagat tggttgtgtc
    22981 gcatagtaga cagcccttat aacggaatca ctctttgtac agggagtatt gcagaagatc
    23041 cagaaaatga tgtatatggg attttagcag aattttgcca aagagatcga atcccatttg
    23101 cacatgttcg aaatattaaa tttttagcta atcgagattt ctatgaggca ccacataaat
    23161 ccgaatacgg ttcattagac atgtaccgca tactaaaaac aatgtatgac aacggattta
    23221 acggatacat ccgtcctgat catggaagaa tgatctggaa tgagacagga agaccaggat
    23281 acggcttata tgatcgagca ttaggtgctg tttatctaaa tggattatgg gaagcaatcg
    23341 aaaaaggaaa caaaagtaaa gataattagt gaaaaaaact gtaattttat tatgtaaata
    23401 agaaggatgt tcaaattctt gatacataaa tccggaggtt gttaataagc attcggattt
    23461 atgtatctta tttggtctag gaagtaactt gattttcttt tttcgattga tccatgagtg
    23521 cttcttcttt taattcaaag atatattctg agatcctcat aatcactttt ttccagtctt
    23581 ctggtgtgat gattcctttt aaaatataga tttttttgat atgagaaggc aattgtttca
    23641 aagggcagtc gctgataacg atgttgcagt tctccataaa atcattttgt attttgatcg
    23701 attcatttga aaaaatttgt tctatcttaa cgatcagatc tttactgata taaaaatcac
    23761 gcgtatagtt cacaccaatc acaattcgcg gctgtttttt gtagcgatca tacaaatgta
    23821 ctaggtcttc aaccatccat tgcacatttt ccttttctaa aataactggt aacaagtgtt
    23881 gattttgttc tctgaaatcc atataaaatt gttcaatggc ggtttggact tctttcaata
    23941 cgctatgttc ttgataaagc atgaaagtag gaaggggcgt atttggttga ttgaagttaa
    24001 gaaatccatt ataaagttta tttcgtgcga atgccaatga gtaaagaatc ttttcttttt
    24061 caggtataaa caaatcaaat acagtacaaa gggactccac catttctctt gaataaagaa
    24121 catgaggaac gtttgctttt tttaaaagtt tgtattgctc caagcttgtt tcttccgatt
    24181 cgattttcgt gatagaaagt cgagctaaaa tattcactaa gatgcgttgt tcgcgacaaa
    24241 ttggcagatt aggtcgcaaa atatctacag agagatcaat aaaaaagtca atatattcat
    24301 actctgtgaa ttctttttgt acgtctttca cagaagttgc aggaaaattc tctttacata
    24361 tttttaatag aaaatataat ttatctatca taccgctatt tagtcggtca agtacttctt
    24421 ttttaaaaaa agtattcatc ataaaagcca ctacttgcgg atcttcatca taaaattctg
    24481 tagcagtact tgaaaagatg ttccaataaa tatctaatgc tgcatattga atttgtacct
    24541 cttttccaga aaaagacttt ctgccattat tagaaaagga gatagacaaa ccgtactatg
    24601 ctaaaaatgc attaatcttt ttcattttcg tatacaaata agaggcacta tagtttgttt
    24661 gctgactgaa actgataatc gaatttgtct gacagctgaa caaagaagtc aagatctgat
    24721 aactgactga ttcgttgcag tatcttccaa atagacgata atagcagtaa gccggatcaa
    24781 ttccttcaaa attcaacttt aaaaaaggtt tttgcttatc aatatgcagt tctgttagat
    24841 tgagacgctc aaagtcttgt tctaattctt tgatgtatag taaaaaattt gattcagaca
    24901 tatggaaatg atctgctaaa tattgtgtag taataaagaa acgatgttca gataggaaaa
    24961 gaaaaatatc taattttgta ttgctttttt tcttcaagat aaacgtttta atcgcttctt
    25021 gagtttgcat gaaagaggct cctttttact taaattgtta aaacttgaaa atattactgt
    25081 ttttttcata aatttagaca aaaaaacagt aaacatatag gtagaatctc tactatctta
    25141 tttcttgcat acaaaaaaat caaggagtga gagagttatg agatattcaa ccatcttaat
    25201 atcaacattt gcagccttaa gcttatcgct tactataaat cagacagtcc acacagagga
    25261 gacagaaaca gccaaatgga cagaaaccaa gtgggccaac gtcaatcgaa ttacctttca
    25321 ggatgaattt tcatccttca attcaggagt cgcaaaatta agaaaaggac aaacagcgac
    25381 catcaaagca aaaattgact attcgggaga cttagcacct atactacaat cagctgttac
    25441 atttgagaat gttgatccca ctttgtcgat tggctatgac gagaacactt tgactatcca
    25501 acacaagcaa gcgatattta ccttcaccgt tacattaaat caggacttgt taaaaccagc
    25561 gctttttact gtcaaggtat cagatggact accgaacgat catcatttaa cgccgtatag
    25621 tcagcggcag acaattgagc aagattcagc catcgactca ggaggagatc ttgttgaaga
    25681 accaacagat aaaccagaaa atgaaaataa gccagaagta ccgcctacag aaaatccaga
    25741 cggagaacag aaaccggaaa tagaacctgg agaagaaccc gatacagaaa ctcaaccaga
    25801 gccagacaat aaatcaaaac ctgaaataac ccctggagaa aaaccagatg taactccaga
    25861 accagatacg gattctggaa accaaacagt accggaaaca aatccagaca cagataatga
    25921 aactgaaaat ccagaaaaac cagaggtaga tccagaagaa aaaccagatg tgactccaga
    25981 accagatacg gacgcaagag accaaggaat accagaaaaa ataaacaaaa aaacaattca
    26041 agaggatgga aaaaaagaga gtaaaaaatc aaatctagca attctaaaaa taaatgaaga
    26101 gcagctgaat aaaaaatcac gaatatttga tagcgcccaa tcagctgaaa ccttaaaaag
    26161 tagcaaggat acaacctttg ctgtcctgaa acgaaaaata aacagctacc aaaatccgga
    26221 gagtcacaaa acaaagttat actgtggagc ggaatcattc tattaagtat agcaacaatg
    26281 ctttctgcaa aaagatttaa acaaaacaga tctctctaac atttgaataa tgtctgtgaa
    26341 cgcatttttc aaataaaaag aaatatccag aaaatgaccc caaaatacca gattgaaatc
    26401 tgatatttgg ggtcgttcat actttatctt tcttcatatt tattttaagg gatatctgtt
    26461 tcttttggaa gatggttcgg gatctttttc actttcaata catctaaaaa gaaggtaaag
    26521 atcggaatgc cgacaatcaa gccccaaacg ccaaagaacc gttcgctaac aagtaagacc
    26581 acaaatgtat aaaaaatcgg cagttctgtt ttgctggaca tcaacttcgg attcagcaca
    26641 taagactcaa ttagatggac aatcaaaatc gtaatcagga tataaacaac atctcggatg
    26701 cctccttggg aataagctat gaaagataga ggaatacaag aaatgataac acctgctact
    26761 gggatcagac ttaaaatgaa aatcatgatc gctaaactca gcagctgatg aaagccaaat
    26821 acagccaagg cgattgtagt gagcaacgta tttaaaagag cgacaacaaa ttgagcttct
    26881 aataccaaac caaatgtatt gacaaactta tcggcaaaat agtaaatatc ttgaaagaac
    26941 caagaaaatt cactttttaa aaacaatctt gagaatacaa ttgttttttt cttttcaatc
    27001 ataaaaaaga aacttaagat gaatgacata gcaaacgcca ctgccatttt tcctatatcc
    27061 tgtaaatagc caagcgcaat cgaagcgcca ttttgaagct gttcaaaaaa atcgtttcgc
    27121 tcaatataag tatggatata ttgtaaaaca ggatctgcat cttttggctg atgctcgtaa
    27181 aaattaataa cggactggat catgtcaaat gtttgatgaa taagcatcgg gatatacttt
    27241 gtgaatgcca gatagataag gtaaacgatc agtgtataaa tcatgatagc taaaacacct
    27301 gtagggattc ggacaaatcg ctgaatgatt tgaacaagat gaatcatcaa ataagtaaag
    27361 ataaatgtga gcaaaatggt tgtaatcata cttctaacta agaaaagaac aaaaacgaca
    27421 aataataaaa cagtgagacg gcgtattttt tcattcgcca aaaaacgctg ataaacggtc
    27481 aaatcgacaa cctccaaata acaaaaaata aacacgttat tcattatatg atggaatatg
    27541 aaaataagaa agagcaaaaa gaaaaaccgc taaatgacgt tatcttatga gttggttttc
    27601 ctcataaaat cgttcatcta gcagtttcag ccctttttct ggtcttgatg aaaattatct
    27661 agcatttttc gggtcagatg ttgcagtgta tccaattctt cctgttcaag tggaagataa
    27721 gacttgatct tttgtggaat accttctgct tgtttttgca acgcagcacc ttgagcagtc
    27781 agcgtaacat ttacgattct ttcatcaccc gtttcacgct gacgattaat ataatttttt
    27841 tctgctaact tttttagtaa gggagtcagc gtaccagaat ccagacacaa atcatttcct
    27901 agctctttca ctgaagtctt tttcttttcc cataaaacta acatcacaag atactgggta
    27961 taggtaagat tcagcggctt gaggagggga gtgtagtgtc taatgatttc tttagaggcg
    28021 gcatataaag gaaaacatac ttgattaact aaatatagat cttccatttt tccacctccg
    28081 ctgttttact atagtgtatc atagcggatt tacacaaaaa agtctattca ctaactgctt
    28141 tcagagtaac ttctatattt ccagctgtag cttttgaata cggacacact ttatgcgctg
    28201 cctctaataa ttcttgcgtt tcttccagag aaagaccttc gatagatcct tcaatctcta
    28261 cacctaagac aaagctcgtt tcactttctg aataaaggga aacttgtgca ctgacagtag
    28321 aagccccaga gatatttttt tgctgtttga tcaattctaa tgcactgtta aaacatgcac
    28381 tgaaaccagc tgcaaacaat tgttcagggt tggttgcatt ttcgacgcgt ttaccaggag
    28441 ctgcaatatt caattcgaat gtacgatcgg gattatgcac ttctccagag cgtccgcctg
    28501 tgttgatcat tttcgtagaa tacatttttt tcataaagtt accaccttta attatatttt
    28561 atacaattga attttacaca atataattgt ggaaagcaaa gaaatggatt ggcataagat
    28621 aaataagaga aagcacatga aacagacgcg ctttctcgaa tagcaaacat atacaaaaaa
    28681 tatcagagac aaaaaataat ctcatcataa acattacata tatttctcaa aaagttgttt
    28741 tctttcttca aaaattactt cacgatggac ctgatgaagc atccataaat aaccaaaagg
    28801 atctctgaac attgagttag tcacaccaaa gtccatcatt tctgtcattt cttgaatctc
    28861 tacacagcca tttcgtaatg ctttattatg tgtgtcctcg atatcagaga cgatgatatt
    28921 gaaccagatc gtatttggat gttcagcagt aggagcagtc aaaccaagag acgtgttttc
    28981 atccagcata tggaatctcg taccatacaa ggtaaaaatg acttcattat tgcctctagg
    29041 gtaatctgtt gcctcaatca gttgtatttc atcaaagatt gtttgataca gttctagtgc
    29101 tttcaaacta tcatttaata caaaatcgat ttccgttcct ttatgcataa agatctcctc
    29161 tccttttcag tcgaacagtt actagattct tttattatac cagctaaaga aaacgcattc
    29221 aaaaaaatag cggatttact taccaactca ttcgattgga tgataagtaa atccgcgtcc
    29281 cattacttct gctacgctgc tgacaatgac aaaggcactt ggatcgattt cttctatcaa
    29341 tggtttcatt cgggaaaatt catgatcagt cacaacgatc aataaaacct cttttgactt
    29401 tcgattatac ccgccttttg cctctagcag ggtaaggcca cgtccaatct cagcacttaa
    29461 gcgaaggcgg atgtcttcca aataatgttc actcatgacc ataacagatt tctttcgatt
    29521 gatacctgtt tcgatataag tcatgacaat tgaagtgatt gcaatagata aaatagcata
    29581 aagaaactct tctattccaa aaataaacag attaagagaa acaacgaatg cgtctgtaaa
    29641 aaataagcca atcgaagtat tcaagtggaa atatttttta aaaatgaggg gagggatcgt
    29701 cgtaccgccg ctagaagagt tgtttttata taaaatcgca actcctaaag caaaaatcgc
    29761 acttccaaaa atgacagaca acaaccgatc tgaagtaatc attgtttctg gaactattgc
    29821 aatgattaaa ggaaaaacca agcttccgaa aagtacttta aaaaagggtt ttttacctaa
    29881 aaatagaaga gctagtacca acataacgat attcaacact aaaataacta tagctcggtc
    29941 aaagcctaaa gcagcttcca gtaatattcc tagaccgctg acacctccag cagcaatatg
    30001 atgaggccct aaaaacatat tgatagcgac tcctaatatg aagagggcaa aaataatctt
    30061 aatgtatgtc catataaatt gtctcgtttc tttcatttat tcacttcctt aattcattaa
    30121 taatactctt atcttattat aacttacttt tcaccttaag ttaactttaa ttaataaaaa
    30181 aattaggcta gtctaaatta ttatgtttca ttcttctaga atatacttct acgccaaaaa
    30241 ctgttaaaaa acttttactt tagaaaattg gagaaattga tcgcttaaga taaattcagg
    30301 acactaaata gtacaggaag tagactaaaa cttttaaaag ttttaagtct atctatttgg
    30361 caagtttaaa aaataaaaat tcggtatcaa aaaactgtta ccgataacat ttttgaaagc
    30421 gttgtctttt gcttctctat cacttatagt gatgacgtca tattaaaaca caaggaggaa
    30481 atcaatggat acagctgcaa tttatcatcg accggaaagt gaatatgcat ttctatatac
    30541 gaatacaaga tttcgtattc ggcttcgtac taggaaaaat gatatcaaaa aagtatatgt
    30601 gctatgtgga gatccatata ccataacaac tgaaaaatgg tatcaaaaac aacgaccaat
    30661 gaaaaaatgt ctaagtacca atgttcatga ttattgggaa attgaagtga cgagcgaaac
    30721 aagtcgctta cagtatgcgt tccatgttgt tggggaagat aacacggact gtttttacgg
    30781 tgatcaagga atttttcctt atcgagaaaa tgtgattact gaaccaaatt tttattttcg
    30841 aataccttat tttcatcaaa tcgatcgatt cactattcca gaatgggtaa aagaaacagt
    30901 atggtatcaa atttttcccg aaagatttgc aaacggggac aaagcaaatg accccgaaca
    30961 cactttacct tggggaagta aagatccaga ccgagaagac tttttcgggg gagatttgca
    31021 aggtgtgatg gatcatttgg attacttgac ggaccttggc atcaatggca tctatttttg
    31081 tcccatattt aaagccacat ccaatcataa atacgatacg attgattatt atgaggtgga
    31141 tcccgctttt ggcgataaaa aacttttaaa aaacttgatt gatgaagcac ataaaagagg
    31201 gatacgtatc atgttcgatg ctgtattcaa tcatatgggt gtccattcac cacagtggca
    31261 agacgttctc aaaaacggag aaaaatcgat ctataaagac tggttccaca tccgtttttt
    31321 cccagtagat tcttatcaga tgactgactt accagaaact gctgaaaata tcccatatga
    31381 tacttttgct tttactccat tcatgccgaa actaaataca gcaaatccag aagtgcaaaa
    31441 atatttgctg gatatcgcaa cttattggat aaaagaattc gatattgatg gatggcgtct
    31501 tgatgttgct aatgaagtcg atcatcattt ttggaaaaaa ttcagagaag cagttacaga
    31561 aatcaaacca gacatctata tccttggaga gatttggcat tcctctcaag cttggctgca
    31621 aggggatgaa ttccatgcag tgatgaatta tgcatttaca gattcgatta aagattattt
    31681 tgcaaaaaag aaaatcacag cttcacaaat ggtcagtgga atgaatcatc agcaaatgct
    31741 ttacagagat caggtgaacg aaggaacatt caacttatta gattctcatg atactgccag
    31801 gatcttgacc ctttgccaag gtaacaaaga attgatgaag tctgtgatgg cctttatgtt
    31861 tcttcaaaaa gggtctcctt gtatctatta cgggacagaa atagggatga ctggtgaaga
    31921 tgacccggat tgccgcaaat gtatgatatg ggagaaggag gaacaagatc tagagctatt
    31981 cggttttata aaagaacttg tttctctaag gaaacagttg tctaagatta tttctgaagg
    32041 ttctactcag tggctaatag taaatgatcg tgaagataag ctctatttca cacggaaact
    32101 agagggacag atcatctatg tttactttaa tcagtcaaaa gaaccttggg tagtagaaca
    32161 ggaaaatgag gtgattctta gtcaaaattg tcagttgtta gaagacggga aagcagagat
    32221 acgacaatac ggatttttga tcatgtgttg aaaactgtta ccggtaacaa caagaaagcg
    32281 tttgcaaatg aagatttgtg cccattataa taagggtgta acagcacgtt gaatacaaag
    32341 gaggaaatga agatgaaaaa aagaagtgtg aaacagttgg gtttagggtt aatctcttta
    32401 ggaatcttag gcggattagc ggcttgtggc aatggtaact cttcagcaga aggggaagcc
    32461 gatgaaggaa aaacgttaac agtttccgtt gattctggat ataaagatta tataaacgaa
    32521 ataaaaaaca ctttcgaaaa agaaaacgat gtgaaaatca agctagtaga aaaggacatg
    32581 ttcgatcagt tggaatcatt agcgttagat gggcctgctg gaaagggacc tgacgttatg
    32641 atggcagcat atgaccggat cggtgcgtta ggacagcaag gacatctggc ggaagtaaaa
    32701 ctgggaaatg aagcagctta cgatgaaaca gacaaagccc aagtgactta tgatggaaaa
    32761 atctacggag aacctgcagt aatcgagacc cttgttttgt actataacaa agatttagtc
    32821 gatacagcac catcaacgtt taaagatttg gagaatctat ccaaagacag tcgttttgct
    32881 tttgagtcag aagcaggaaa aaatacagga tttttagcta aatggactga cttttattat
    32941 tcctatggtt taattgcagg ttacggtggc tatgtatttg gtgatgatgg cacaaatcct
    33001 tcagatatcg gtttgaataa tgcaggtgct gtagaaggaa tctcttatgc aacagattgg
    33061 ttcaaaaatg tttggccaaa gggaatgcaa gacataaaaa gtgcaggaga cttcgccagc
    33121 cagcaattca tgtccaataa aactgcagca atcattgacg gtccatggca agcgcaaaca
    33181 tacaaagaaa acaatatcaa ttatggtgta gccaaaattc ctactctaaa taatggacag
    33241 ccttatcaac cgtttggcgg aggaaaagga tgggtagtca gcaattatgc taaaaacaaa
    33301 gacctctctc aaaaatggct tgattatgta acgaatcagg aaaatcaaga aaaattcttt
    33361 gaaatggtaa atgaaatccc tgcaaaccaa caagctaggg aaacagcaaa ggctaaaaac
    33421 gatgaattaa caacagctgt gattgaacaa tatgaaacag ctcaagcgat gccaaacatc
    33481 ccagaaatgg gcgaagtttg gacaggtgca gaaaacttga tgttcgatgc agcatcaggt
    33541 tcaaaaacac caaaagaatc cgcagatgaa gcagtaaaaa caatcagtga agccatagaa
    33601 caaaaatact gattaaaatg ataaccgtcc atttttataa aaaatggaac aggtgcttca
    33661 gataaaagtg gcaagagcga gatggaaaat tatcttgctc ttgttgccat tatcttaaaa
    33721 gcagtttgcg gtttagcaag tttagagggg gcattcagat gcagcaagat aaaacaagca
    33781 atatcaacga aaaaaaaatc acgctttgtt cacttattcc agggatgggg cagtttgtaa
    33841 atggccaagc ttttaaaggt atcgtttttt tcgccatatt cgtcctattc attggtcaga
    33901 tgcttctggg cggatttcat gcattggtag gattggttac tttgggaagt ataccaatgg
    33961 aagatcattc attatttatt ttgattgaag gcactttgca attcatcatt acaatcattt
    34021 tcttgatttt ttacggtttg aatcttttag acgcttatcg agtagcgaag atgcgtcaag
    34081 agggcaaaaa aatcaatcgg acgatgaagg aagtcataca taatataggt gatgaaggtt
    34141 ttccttatct attgacactt cccgcttatc ttctcatgat ttttacgatc attttcccag
    34201 tcctagttac attgttcact gcatttacta attatgactt caatcatatt ccaccagcca
    34261 gtttaattga ttgggtaggg gctaaaaact tttttagtat cttcttttta agctcatatc
    34321 gtaatacttt ttttgcagtc ttttcgtgga ccgtgatttg gactttatgt gcaacaactt
    34381 tgcaaatcac ccttggtatt ctcacagcgg ttgttgctca ccagaaattt attaaattca
    34441 aacgtatttt cggtatcatt ttcttattac catgggcggt cccagctttt attactatca
    34501 tgagtttttc gaatatattc aacgatagcg taggtgccat caatactcaa gtgattcctt
    34561 tcataaacca gcttcctttt gtagatatca gtgcaatctc ttggaaaacg gacccgaact
    34621 ggacgaaagt agcaattatc ttagtgcagg gctggttagg atttccctat atctatgtga
    34681 tggttacagg aatcttacaa tcgatttcag aagacttata tgaagcagca aaaatcgacg
    34741 gagcaaatgc atggcagcgc ttttcaaaca tcacgcttcc tgctgtcttc ctagtggcag
    34801 cgccaacatt tattacacag tatacgggga atttcaataa tttctccatg atctatctgt
    34861 ttaacaatgg cggtcccgga agcgtaggcg gaggagctgg ttctactgat attcttattt
    34921 cctggatcta taaacttacg actggaactt ctccgcagta ttctatggct tcagcaatca
    34981 cgttgattat ctccgtcatc gttatctgtg tgtcattgct ggtatttaaa aagacaaatg
    35041 cgttcaatat gggggattaa caatgaaaaa atttcattcg agtgctcgat tccaaaaacg
    35101 actttctcac ttttttactt acttttttct tttaatactg tcagtcatta tcatctatcc
    35161 gcttttgatc acagcaagta ctgccttcaa gtcaggaaat attgcagctt ttagtttaga
    35221 tctttcaagt gactggactt taggaaactt tactcggcta ttcagtgaaa cactttacgg
    35281 gacttggtat aaaaatacat tgatcatttc tgtcctaaca atgatcgtac aagtgactgt
    35341 agtaactttg gcgggctata cttatagtcg ctatcgtttt atcggccgca aatcaagttt
    35401 gatgttcttt ttagtgattc agatggtacc gactatggca gcattgactg ccttttatgt
    35461 catggctttc ttattagaag cgctcgataa gtactggttc ttgacaatga tctatatagg
    35521 cggtggaatt ccaatgaata cttggctgat gaaaggatac tttgacactg taccaataga
    35581 tttagatgaa tctgctaaac ttgatggggc agggcatttc aggatttttt tccagattgt
    35641 attaccgctt gtcaaaccga tgctcgccgt tcaagcatta tgggctttta tgggaccttt
    35701 tggggatttc atgttagcta agttcctgct tcgaacacct gaaaatcaga ctgttgctgt
    35761 cggcttacaa acatttattt ctgatgtgac aaaccaaaga gtctctcttt ttgctgctgg
    35821 ggcaattttg attgcactac cgatttgtct attattcttc tttttacaaa agaactttgt
    35881 taccggcctg acatctggcg gaacaaaagg ataggagcag agtatcatgc aaacatttcc
    35941 gttaaactat ttttccgcat tacgtacgcc aactcaagtg tttgcgggac gcaaactatt
    36001 atcatggcct aaatttttct tgatatttgt atttttagtt tccttgatgg tcatgcctgt
    36061 gactcttttt tatgccaatc agatccaagc gattccatta gaacaatttc tatccgttca
    36121 ttcgctgatc gatgaacaag gaacccaaaa attttctgaa cttgaattat ctgaaactgg
    36181 gttacaagca tcgcaacaaa cgatagctgt gacccctgaa attttggtag gtgtgtcttt
    36241 atctgagaaa caacaatccg atcatggaac ttttattgat tttgagaagg aacaatgggt
    36301 gatccaacaa aaggataaat ctggtatacg ccgctatacg atgaattata gcccatcttt
    36361 ccaaccagat tctgtacgta cgcccgaaga tttccaacga tttttagaaa gagaatttta
    36421 tgccagtaat cgtccaacaa tcattttatc ttatagttta agtcttggtc ttgtcctatt
    36481 tgtcatgacg agtctcattt tattcggggc ttcctttttc ttgtggatga caagaaaaag
    36541 ccaactttca tccattcata cattcaaaga gagtgcaaat ttgatgctga atgttatggg
    36601 aataggttcc atggcagcag cagttgtagg gtttatccac tttgacttta tcttgatgct
    36661 aagtgtccaa acaacgataa ctgtcttgct gcttttatgg gtttttgtca aaacaagatt
    36721 caaagataag cgtgtcgaat gacaagttta agcgatttat gcgacaatga atgtgaaaaa
    36781 aatgaaaaat aaagtagttt ttgaaaaatt tctgaaaaaa ttaaacttct tagttgattc
    36841 ttttcttttt tagtattaac gtaatagggt agttttttta aggaggaaaa tcatgttaga
    36901 gaaaacttta tacaatcaat tattttcacg ttctttctcg ctccctgtcg aagtaaatta
    36961 ttgggacggt acaacgaaac aatacggaga tacggacact cctccccaaa tcaaaatcaa
    37021 tatccatgaa gaaatcccaa tgaaagagat tacaagtaac gcttctttgg cactaggaga
    37081 agcctatatg gatcatcgaa tcgagatcga gggaagtatc caagcattga tcaacgatgc
    37141 ttatcagcaa gcaaacagct ttttacgagg aaaagattat ctaaaatggc tgccaaagaa
    37201 agaaaaacat acgaaacaaa aaaacaaaga agatatccat gcgcattatg acttaggtaa
    37261 cgacttttat aaactttggc tagatccaac tttgacttat tcttgtgctt atttcaatac
    37321 acctgaagat acgttggaac aagcacaaat caataaagtc catcatatct tagacaaact
    37381 ctttatcaaa gaaggcgaaa cgttgcttga tatcggttgt ggctggggaa cattgatgtt
    37441 cactgctacc aaagagtata atgtcaaggc aactgggatc acactaagtg aagaacaata
    37501 cgatttgatt accaaaagaa taaaagaaga acatttagaa gataaatgcc gtgtattatt
    37561 aatggattac cgcgaactaa aaggcgaaac gtttgatcat atcacaagtg taggtatgtt
    37621 cgaacatgta ggttctgaaa atcttgaagg ttatttcaaa gtggtaaaag atttattgaa
    37681 accaaaagga acagctttga tccatggaat cagccgacaa caaggcggtg cgacaaatgc
    37741 ttggatcaat aaatatattt tccctggcgg ttatgtccct ggtgttactg aattagttga
    37801 taatattaca aaaaacgatt tgcaactgat tgatttggaa agcttacgtc gtgactacca
    37861 attgacatta gaacattgga caaagaattt tcatcaagtc caagatcaag tcacgaaaga
    37921 aatgggtgat tccttctatc gaatgtggga tctatattta caagcttgtg cagcttcatt
    37981 tgaatcaagt aatatcgatg tgattcaata tttgcttgtt caacctggaa acaatgacat
    38041 cccaatgcat cgtgcataat tgaggcaaaa gtactgttta aagactattt aaaaagaagc
    38101 taagttttat gatgctccag aagtgaactt ttttgtctaa cttctaggat tgtctctaaa
    38161 gcttagtttc tttttcttta tattttcata ataaaagttt ggttctttaa tttttaaaca
    38221 tgacagaaaa ttttttcttc gttatacttg taatagaata ctattttata acaaagggga
    38281 tcgaatcaat gaaaaaaact gttatttata ctagtttatt tgcagctttg tttttagctg
    38341 cttgtcaatc aaataccaat caaggcacat ctgggaatac gtcttccagt agcgaacagc
    38401 gctcttcaca ggtatcggac tcctcaacaa aagcaagcac atcttcgtct agccagacaa
    38461 aaagttctag tgaagcaagt actcagtcag atatcaaagt gtctgcagct cgtgcgatcg
    38521 aattgttcca agataaatac ccagatactg caattactag tttggaatta gatagtgatt
    38581 ggggatctta cttctacaag atagaaggtg tcgatgatca aaaagaatac gaagcaagaa
    38641 ttgatgctca aaatgaaaaa atggagacag agaatccaga aactctagat cgagatgaac
    38701 aaaatggtca aaagaaaaaa gaagatggtc tggatgttga taaattgatc acgattgagg
    38761 aagcagcaaa aatcgccgtc gacaaagtag gaagcggtac ggcaacagat tgggatttgg
    38821 ataaagattt aggtactact tattgggaag tcaaagtaaa agacggcaat caaagtacat
    38881 cagtcaagat caatagccaa acaggcgaag tactgtcaac tgaacaagat gattaagaat
    38941 ttcttcggaa aacaagagtt gatttttctt ttagtcatga tacactagac aaaataactt
    39001 gtttgaagga gcgatttact tgagtttaga aacgaccttt gccaatcata tctttgccaa
    39061 tcctttgatg aatgcatctg gtgtccactg catgactacc caggaattag atgaactggc
    39121 tcattcagaa gcaggagcat ttatcactaa gagctgtacg atcaatgaac gaaaaggaaa
    39181 tcccgaacca cgctattttg atgttccttt agggagtatc aattctatgg gacttccaaa
    39241 cttaggtttt tcttattatc tagagtatgc tcttgcgtat gaaaaagtgc aggaaaacca
    39301 aaatcaaccg ttattttttt ccattgccgg gatgagtgtc caggaaaact tggaaatgct
    39361 tgagaaaata gaaaaaagtg ggtttaatgg gatcactgaa ttaaatttat cttgtcccaa
    39421 tgtgcctgga aaaccacaac tagcttacga ttttgaggcc acttatgaaa cactgaaaga
    39481 agtattcagc atcttttcta agccgctagg aatcaaattg cctccttatt ttgattttgc
    39541 tcattttgat cagatggctg atatcttgaa tcagtttcca ttgacctatg tcaatgctat
    39601 caacagtgtg ggcaatggtt tgtatatcga tacagaacaa gaagcagtag tcatcaaacc
    39661 aaaagaaggg ttcggcggca ttggcggaga gtatatcaag ccgactgctt tggcgaatgt
    39721 tcgcgctttt tatacacgtt tgaaaccaga aatccaaatc attggtacag gtgggattcg
    39781 gacaggacaa gatgcatttg aacatctgct atgtggcgca agcatgttac agattggtac
    39841 cgagcttcat aaagaagggc cggaaatttt ttctagaatc ataaaagaat taacgcagat
    39901 tatgtcagaa aaaggatata cgtcaattga cgaatttaaa ggcaaattac gcaccatttc
    39961 atagcaaaga gacgaaggtc atgattgatc ttcgtctttt tttataaaaa acactcgtct
    40021 gttcattcct gtgcaagcgt actcattgta tgttagacga acttgtggga aaataacaga
    40081 ggcaaaaagg aggataatat gaatgatgca acagaattag caaaagagct taaagaaaaa
    40141 gaatttagtt ttcaagaact agttacggat atacagaaaa aaataaagaa gcaaaatcat
    40201 gaattgaatg cgtttgtcgc gcttgaacca ttcgagagta attcccacga gctattgatt
    40261 ccccagtcag aaaaagggcc attggctggt atcccatttc ctttaaaaat gctcggtcaa
    40321 gaaaaaaagg gatggctagc tacttcagga tctcgtttat ttgaaacaca ccgagcttct
    40381 catacatcta attatgtgcg gcaagcagaa gcgataggtc tagttccttt tggacaaacc
    40441 aatgcgccag agtttggttt caaaaatatt acagacccag tcatctatgg cccagcaaga
    40501 aacccttgga accttgaatg tacaccgggc ggatcaagtg gaggagctgc tgctgcagtt
    40561 tcatcaggaa tcgttccttt agctggtgct agtgatggcg gtggttctat tagaataccc
    40621 gcttcgtttt gcggattgat tgggctgaaa ccttcgcgag ggacgatgcc agttggtcca
    40681 tatgcttgga gaggatggca aggagctgct attgatttcg gcttgacagt ttctatgaga
    40741 gacacggagg ctttatttta tgggatgaga agtatccatt ccggtgcgcc atatcaagta
    40801 tcgccagttg agtggcaaac acatccaaga aaaaaacggt tgaagatagc tttttgtgtt
    40861 gattcaccga tccactcaca gattagtgaa gaggcagtac ttgctgtaaa aaaagctgta
    40921 gatttccttg gaaaagaagg gcatgatttg acagagattc cttacccact agatggtcgt
    40981 aaactgatca cgtcttatta tcagatgaat ggcgcagaaa cagcagcaat gatccgctcg
    41041 attgaaacag gcatccagcg agctgtcaga aaagaagaac ttgaacccat ttcttggact
    41101 ttgttccaat atggtcagaa gctctctgcc gctacttaca tcgattctct gcatgtatgg
    41161 gatgaagcag caattgtcat ggaaaaccta catcaagaat acgatctatt cctttcacca
    41221 acgactgcat ttaccgcccc taaaatcagc gaagatttgc aaagtgatgc gataagagca
    41281 cagatggaac acatcagtga ttgttctgaa gtagaaggtt tagacgttgt ttcttccatg
    41341 tttgaggaga gcttgaagat cacaccttat acacagttgg ctaatctgac tggacaacca
    41401 gcaatcagct tgcctactca tttgacaaaa gaaaacatgc ctctaggaat ccaatttatg
    41461 gctgccagag gaagagaaga tttgctattt caaatcggct atctatttga agcaaatcat
    41521 cagttccatt taccaaatcc atggaaatag cagaaacaag gagaagaaga atgaagaaaa
    41581 aaagattgtt tgctctaata actagtgcct tttttatttt aagcgcttgc ggtacagctg
    41641 ccgctaatca ggaagataac agctggagta atgtagaaaa aaagaaagaa ttagtagtag
    41701 cgacttctgg cacactgttt cccgcatctt attataatga tcaaaatgaa ttagttggtt
    41761 atgatgtaga tttggcaaaa gaggtggcta aacggttaaa tgtcaaaatc tcctttaaag
    41821 aatacaacgt ggatggacaa atcacttctg tttcaaaaag agaatcagat tttgctgcga
    41881 acgattttgg tatcacaaaa gaaagagaaa aaaaattctc cctttcagag actttcaaat
    41941 attcatttga cagtatgatc gtacgaaaaa aagatgattc aggaatccac tcacttgaag
    42001 atcttaaagg gaaaaaagca gctggcgaac ccaatactag ctatatgcgt ttagcggaaa
    42061 aacacggagc taaattagtc acatatgaca atgccacgaa tgatcagtat ttaagcgatg
    42121 tagcaaatgg tcgaacagat gttattctaa atgattatta tctgcagaaa atggccgtat
    42181 ctgctttacc agatatccct gtaaaaatac tggagaacgt ttatttcaat gaaaacgaaa
    42241 gtggtttttt attgaaagaa ggaaatgatg ctctgaaaaa gaaaatcgat ggcgtattaa
    42301 aagagatgaa agaagatggg acgacaaaga aaatatctga aaaatatttt caaactgatg
    42361 tttcagtcaa accgaaagaa acgatggctg agtcggtgag tgagtgatgt actttcctct
    42421 aattgatttt tccctcatgt ggacttcact tcctttcgta ctgcaaggct tggtatacac
    42481 attagggatt ggcttcgtgt cgtttctttt aggaaatctg gtagggttgc ttttgacagt
    42541 tttaggtctt ttggattggc tgcctctgaa tgtatttatt cgtttttatt tatcgttctt
    42601 tagaggcatt cccgcactag tcctgctgtt tctgttatat tttgggttac cttatcaatt
    42661 aagtgcctta acggcttcag tcatctgctt tacgattacc agcagtgcat ttattggtga
    42721 aatctatcga ggatcactag caggcgtttc ttcaggtcag tgggacgctg gtttagcttc
    42781 tggtttttct ttctttgggg tattgaaata tatcgttttg ccgcaagctt ttcgaatctc
    42841 agtaccggca ctcagtaacg tagctatgga tctagtgaaa ggtacttcgt tagcagcaac
    42901 aatcacagtg ccagaaatat ttcaaaaagc aaagatcata ggcggtcgga cattcgatta
    42961 tttttctctc tatattttag ttgcactgat ttattgggtc atttgtatcg ggatcgaaaa
    43021 agtgcaacag tatttagaaa aacgttctat gcatcagtca atctgaaaaa tagaaatggt
    43081 gctagcgtgg ctggcaccat ttctattttt tgtttcttgt atattctgcc tagtatttta
    43141 aaaccatttc atgtaaaatg taaatcgaaa atatgttcgg aggtagaaaa catgaagacc
    43201 ccgctaacca tcgaaataga atcctcgtta taccaatatt gcatcgagca aggcggcttg
    43261 gttgtagaag aagtcacgat gcctgatgac caaggaattg tagatacgct tagctgcttg
    43321 acaaaatttg atggaagccg agaatggcga tgctatgagc tgaaagtgac aaaagcagat
    43381 tttcgctcta cagccaaact gtcatttgtt ggacattata attattttgt tttaccaagt
    43441 aaactatatg aagaagtaaa aactgaaatc cctacaggaa taggtgtttt agtgtatcgt
    43501 ccgtacacgg ttgaagagga gatgcctgcc tctggtacat ttctgattgc gaaaaaagca
    43561 gcaaagaaag agcttgctgt accggaagaa gctttgacaa accgcttcat ggcttcttta
    43621 ttcagggaag taagaaaagc aaagcaaatg gagcgaggcg taaaatattt tccaacagat
    43681 caattgtaca aagaattgaa aagacgttca gaaaatcaag atccattttc taacgaacga
    43741 tattatgaac ggtttttaga ggaaatagct agtcaggcaa tcaatgagtt gcaagaagaa
    43801 gttatcgcgc ttcagcatga ttatgaatat ttgaggcaac aacagcagat ccgtcggttg
    43861 cctacagagc cattggagtg aaaaatatgt actatcctta tttaagagga aaacaatttg
    43921 acctattagc attaaaagaa gcactgtcgc gaggattgct ctccaataaa atccaaccag
    43981 tgatcgaacc agtacgtgat tccgctacgc ttaaaaatgt gatcgaatta tttcaaaaaa
    44041 agcaccatcc aatcgctgtg atccaaaatc cccaagtagg acaattcaaa ttattcgatc
    44101 agcatgtgca cagttggaca gtcaaagaaa atagctctgt cgtccctgca cagatcttaa
    44161 cgccagaaaa tctcagtgaa gtactggatt cacctccagc gtttcttgtt ttcgatggac
    44221 agcactaccc aaaagataca gaggtgtgga aacagttggc aggagttgat tcgaaatttt
    44281 tgataccaga tactagtcgc tttcgcatct ggctgcccga aaacaaaatt gtgatccgtg
    44341 actcttttca aacgagaaag catgttgaaa gttatgcaga taaaacagat gactttttta
    44401 gtgatgacta tctttttttc cacgcagacg gatatattgg attttctgat ttcacgatcg
    44461 aaggtagccg ttatttcgat aaagggttcc ctagccgagc gcttgctatc catctaacgt
    44521 atattgatgc ttacggcaat atacgcgtga aacattttgt ctctgacagt aacgactctg
    44581 ctaaagacca agcattgaag tttcttgaag caggagataa aatgaaaaaa tggatcatgc
    44641 gtcaccatca tcaactcttg attacttcag gaatgattga attattggct ctttaccagc
    44701 aacaaaaatt ccctggactg ggagtgttga aaaagtggtc acttatgcat catttggaac
    44761 ttattagtca cctgctagat catccaaaag attggcttaa atattattca aaagttcagg
    44821 aactatatga agtgatccaa tgattagata atgtaaatga aaaaggaata gaaaagagga
    44881 aactggatgt atattcggca aacaaggaaa gaagaagtag aagaagtggt agaaatcatt
    44941 aatgccgcag cggatttcat tgcttcgcaa ggctctcctc aatggcaaaa cgggcagaaa
    45001 ccaacaaaag aaacgatttt gaacgatatt gaaagaaaag aaagctatgt attaatagtg
    45061 gaagatcaaa ttgctggaac agctgcatta gtctcaagcg tggatcccgt ttacacaaaa
    45121 atagatggta gttggaaaga atcaactggt ccttatttat cgattcatcg tgtcgcttta
    45181 gcagaaagta ctcgaggaaa aaaactagca aaaccgttct tagaacattt gttgacgatt
    45241 gcagccagca aaaatatcca agatatccgt atcgatactt atccagctaa tgaaccgatg
    45301 gaaaagacca tctattctgc tggattcact tatcaaggga tgattgaatt tccttttgaa
    45361 cacggcgaaa gaaaagcata tcaaataatc atcaattgac tataaaatca tctgagcaaa
    45421 gataaagaca ttaacactac tgaaaaaaac aatcatatga tagccaagag gttgtaacag
    45481 aagtggccag cttcaaaaaa caagaaagaa tttccgaaaa tgcttctcaa atttttgtgg
    45541 aattttagcg tattttacga aatagctgct ttctgaaact cccttcatcg agaggtcgtg
    45601 acatagcttt tgtcacgacc tctcggctta tcctacgagt attattcgat acatttatat
    45661 caataatcct ttttttattt tatctgcagc ttcttggaac gctccatcgt ttgacacgac
    45721 taaaagtgag gggatagctt tcaaacgttt caactgtgct aaatcttcta cataaaccgc
    45781 cagtctggct gaaatctgtt cagatgaatc tccacgtttt ttcaggcgcg attgcaatgt
    45841 ttctttagag atcgtaataa agataggacg gactaccgtc ccgaaatact catataaatg
    45901 ccaaaaacca tttgctgtca aaacggcgta acaatttcct actgtcagtt tctttttaat
    45961 ttctgcttta ctggaaccat atttatgttg agcatattgg tcccactctg caaactcatt
    46021 tcccttgatc atctgattga atgtattgtc agaaacaaaa taataatcct cttgatcttt
    46081 ttccccaggt cttggtgatc gattcgtgta agtaatgatc ttttgttccg gctgaaaaat
    46141 agcttctgcg agtgaagttt ttccagaacc agaaggaccg ataaatacat agcaataatt
    46201 cattgtcatt tctcctttga tgattgaaca agtgaaaaag ctttggaaaa tatttccaaa
    46261 gcttttaaaa gtaactttgc ttttttgtat attataccag caagccagtc acaatagcac
    46321 ttaaaaagct gactaaagtc gctgttagca aaattttcaa tccgtgtttt gcgacaagat
    46381 ttcctttttc ttcatttaat cccttcatcg caccagaaat gatcccgata gaagagaagt
    46441 tggcaaatga aacgaggaag acagatataa ttgctgttgt acgctcagta aaatggaaat
    46501 taccatttgc taattctgtc atagcaacaa attcatttgt aaccagttta gtcgccatga
    46561 tactaccggc atccactgct tctttccatg ggattccgct gatgaaggca agaggggcaa
    46621 agatgtaacc taggatctct tggaaagtga tgccgaatat cccgtggaag atagcattga
    46681 tcatggcaat caaagcaaca aaaccgataa gcatagcagc aaccgtgatc gccacatgga
    46741 aaccatcaag aatgtattct cccaacattt ggaaaaatgt ctgttctttg ttttcttcaa
    46801 tgatcagctc atcttctttt tcttctaata catagggatt tacaattgaa gccagaatat
    46861 aaccgccaaa taagttcaaa acaagggcag tgatcacata tttagaatcg atcagtgcca
    46921 tataagatcc gacgatcgac atagaaactg tagacatagc cgatacactc atggtgaaca
    46981 aacgtttctc agataggaag ggtaattcct tttttatgga aataaaaact tccgattgtc
    47041 ctaaaatagc agaagctact ccattataag attccaattt gcccatcccg ttgattttac
    47101 ttaatcctag accaacagct ttcataaata atggaagaat cttgatatag cgtagaatac
    47161 cgataatagc agaaataaat acaattggca tcaatacgga aagaaagaaa ggtgcacttc
    47221 ctttatttgc tacaccggca aaaacaaagt caacaccttg cccagcaaaa gcaagaagat
    47281 gatcaaacat ttttgccaat acagaaacaa ccgctgtacc aaaagttgta cgtaataata
    47341 cgaaaccaag aacaagctgt aaaacgaaca tcacaattag tggtttcaat ttgattgctt
    47401 ttttgttact gctgatcaaa aaagcaagcc ctaatgtgac aaataaacca ataataccta
    47461 ataaataact caaataatca ctcctattcc aaattccgtt ttattttacc tgttttgcaa
    47521 ataaaatact aatctttagt gaaattttca ttactttttt agagtgaaac gcattcattt
    47581 tccattagaa aataaagcga acaaaataaa aggtttaaaa agtgtaacgt cttagtaaag
    47641 tctataaaaa aacgaaacta tcgattaagc taatagtttc gttttcaaca tttataagta
    47701 aactttttct attggtaata tagattttcg gaagggtaga caggtgcgca ggtaatatcg
    47761 attcctagtt ttttcaatgt ttgttcatca tttttattga ccatcacagt agagtgggct
    47821 tgtgctccct caagttcagc tagtttatca taagcaactt gagcgattgg attcgttact
    47881 gcactgattg ataaagcgat caagacttca ttgatgctta gtgcatccaa agaggaatga
    47941 agtgtctttg atttcaatcc ttgtattgtt tcaagaacta atggtgacaa taaaggaatg
    48001 tcatctgcga tatgggctaa atgttttact gcatttaaga taactgccgc tgacgcatcc
    48061 atcaatgaac ttgtacgccc ggtaatgatg acttcatctg gtaattcgat tgcaatcaca
    48121 gcagaaggat cttttgtagg actttgcgct tgaatcttat ctgcatagtc atgggcaggg
    48181 actaccacgt ttcggtcttc aggacgtaaa ccgacttctt ccataatcag tttgattcga
    48241 ttaactgctt cttcgtcgat caatcctttt ttgaagtcgc attctgtttg gaaataacga
    48301 cggatgattt cttgtttaga ggcttcttgg acaacggtat catccacgat cccaaatcct
    48361 acacgattca cacccatgtc agtaggagat tgataaacag attctttacc agtaattttt
    48421 tcaataatac gtttgattac aggaaatgtc tcaacatccc gattatagtt cacagctact
    48481 ttattgtagg catcaaagtg gaaggaatcg atcatattga catcttttag atcgactgtg
    48541 gctgcttcat aagcaatgtt cagcggatgt ttcaacggaa cattccatac tgggaaagtt
    48601 tcgaacttgg agtaaccagc tgcatgtcct ttttggcttt catgatacag ttggttcaag
    48661 gaagtagcga gctttccact tcctgggcca ggagctgtca ctacaacgat tggtttagtt
    48721 gtttcaatat actcattttt tccaaaacca tcttctgaga cgattttttc tacgttgact
    48781 ggatagtctt cgatttctga gtgtttatag accttgatgt ttcgtctttc tagtttattg
    48841 ataaagactt tcgtggcagg ctgtccacta tagcgagtga taacgacact gttgattaat
    48901 aagccataac cacgtaattc atcgattaac cgcaagatat ccatatcata agtaatccca
    48961 taatcaccac ggatcttatt acgttcaata tctccagcat agacgcaaat cagtatttca
    49021 gcttgatctt tcaatttttg cagcaatttg attttcgcat cttcttcaaa tcctggaagt
    49081 acacgtttcg catgcttgtc gtcaactagc ttcccaccaa attctaaata taatttgtca
    49141 tagtggttca ctcgttccaa aatatacgct gactgctctt caatatattt ttctgaatca
    49201 aaaccaattt ttttcactag gttccctcct taagtactaa agcatttttt catttttttg
    49261 aggattcgtc aactggaaga gcgaaattcc tcaaaaaaac aaagaaaatg gtttaaacca
    49321 acttgtctag atagttattc attggtatga tagtttttga taaggaaggt gctacatgaa
    49381 aaaaagacag aaaaagaaaa acgcatataa acactatatc cgttcgattt ttaccggtta
    49441 tgaaagaatg cttgaagatc ccgaattgga acaattgacg tttacttatc taaacgaaga
    49501 aacgcagcta actcgtgatg agcatcagcg cattcatttc acaacaagag atctgcctag
    49561 taaataaaaa ataagataaa ggtcatcgga ttcgattctg tcctatcaga tggaaagact
    49621 agttgatcga aaaaaaggct gtaccaaatt ttggtgcagc ctgttttatg gagcattgcc
    49681 cgctcttttt tataacaatt ttttacagaa aatggtacta ttgaagtatt aataagtgaa
    49741 aaacagatga gtaagaggag gcaagtagta tgaaagagcc agatacaaat gcaaattatg
    49801 agccagacga actagaggag acgaatgcgg atcaggatac aggacaagaa tcgtcttctt
    49861 tttacgatat caaagaaaaa gcaagcatga atctttccca attattagaa gacttacagg
    49921 cttttgaaaa agctgtccgc gaagaagata tccctgagat ctaccgttta tacaatggcc
    49981 gattgaatga agaattaaaa agaagctcaa atgaaaatca cgagatcgat caattgctga
    50041 tgcgtaagat ccatgatcgt tttttacagg aatttccttt tatggaacaa gtagaaaaca
    50101 tctcgccaac gatgagctat tacaaaatcg gcacttatta ccatgatcgg cccacggtcg
    50161 gtattgatgc aagtctgcca gaaatttttg ttttgcctaa gatcgatgaa gagtgggaga
    50221 aatattccca accggagtca acggactatg acaaaaaaat caatgagcta gacgcaaagg
    50281 tgatcactgc tcagacagaa attgaacgtt tagatgaaca aataaaagaa atcaatcgac
    50341 aaatgactga tttgaacgac aataaaggat tcttgaatcg taaaaaaatc gaggaagaaa
    50401 ttcaagaatt agagaagaag aagcaagtac tatctaatga aaaattaggc tggcttcctt
    50461 atattgagac gcctgaaaca atccagcagc aaaaagaagc attgaagcaa gaagcaagag
    50521 cagaccagct tcgtgcagcc atcgtagaaa aagaacaaag gcaaatcaga cgctatttcg
    50581 gtagcaaaga gggattcggt caagcaatcc atgaattctt gatgaattat cttggcaatg
    50641 agaatcctaa taatcaatct aatgaaggag gttccgagta tgaatgaaca cgatgaagca
    50701 gaatggacaa aaaatgaaga acaaacagaa gatcatgaag aaacgaacac ttcattggaa
    50761 gaacaaacaa gtaatcctct ggatgacaca gaagaaatcc ctatgctaga ggaaacaaaa
    50821 atacacgaaa aagaaaaagt aactccgacg aatacgaata ctgcagaaaa cacccatgct
    50881 tacgaaataa ctgaaaatcc cgggtattta gaaggaaaag agcaagatca tcaagatatc
    50941 gtcagtttga ttgaaacagc ggaagcagaa ttagcggatt tacgtatgag aaaagatttg
    51001 atcgaatcag attttccaca attattagaa tcatatgatc gttttatatt gaatgaagaa
    51061 ccaatctcca cgatcggaaa gacaagcctg atcgaatact tcacgtatga attactgaaa
    51121 ccgttgaatg atattggtct ggaacggtca aattcagctt atgacacatg gaaaacaaga
    51181 catttttcga tcaattatac gttgaatgga caaaacgaat tagtcttttc ctttgtatta
    51241 ccgaccatcg atcaacgata ccaagtggaa tcgatgagtc tcctaaacgt atcaccagag
    51301 acaatggaaa tcaatgtcca agatgaccgg gtgctttctt tgatccgaca ctggtcagtg
    51361 gatcgagtat tctctgcagg gcagatcacg atattcaatc acaaattgaa tcaattatta
    51421 gcccatgcaa gaacacttgg ttttaccgtt aatcagacat tactagacaa tacgaagcca
    51481 cttcgtttga atcttcagag cgaatttgaa ttgacgaatg aagtattgga tgatattttt
    51541 atcgtggcaa tgaaaaatcc atcctacgat atggaaaaga tcaacgaaac tacttatcaa
    51601 gtacgattag ataaaggaca gtctttaacg ataacaacaa acgaaaaaga tcaaacagtc
    51661 ttgtcgatct catctggaga ctacccacga tctgtcattg atttctttat caattacgaa
    51721 ttcttggtcc cattaatggt gagaaagtcc tgattggata agttggatta ccagatgtca
    51781 ttcagataat tctgttccaa aggacttgcg gcagttatcc agaataattg tcacaagtct
    51841 tttctgtatg tactaaatgg acgtgaataa cttgctgatg aaggagggat ttcattagga
    51901 atgtccacaa ataaaatagc acagcttagc ttattgtctg ctgcttgtgt agcaggtagg
    51961 atcgcttttc aatttattcc gaatttccag ccaatgactg ctattttttt atttatagta
    52021 ctttacttaa gcttaaaaga tgcgttaatt gtcatgagtc tttctattgc gatcagcagt
    52081 ttttattttg gtgttggacc atgggtcatc ggccaatgga tcagctatac tgttgttctg
    52141 agtttgtttt caatcgtctg cttgagaaaa acagtgcaaa ggaatctatg gttaaaggga
    52201 gtctgttttt ttctggcggg catggtttac ggaataggta tgaccgtttt tgatacgctg
    52261 ttatatcgtt tgcctcaacc ttggatctat taccttcaag gggtgtcgtt tgatttgatg
    52321 cacagtatag gcaatgttgt gttctttctt gtttttctgc cagtcgtcaa acgtttttac
    52381 aatcattcag gaggaaaaaa tgaaaaaaat aatttatagt ttgcttgcaa tgagtgtcag
    52441 tatactcatt ttgacaagtt gcagtacaac ctatacacaa tcggcttcca accaaaagga
    52501 ggaagtaaaa acatcgcaaa aagcaacaat cacgttacaa gaaaatggtc aaaacttttc
    52561 ggaaaaagaa gtgttcttca aaaaaggcga tgatttactg agtattttga aacgaaattt
    52621 tgaagtcaaa gaagataacg gcttcatcac ttcgcttgaa gggcactcac aggatgagaa
    52681 aaatcaactc tattggatgt ttactgtgga cgggaaaagt gctactactg gtgccaaaga
    52741 gatcaaattg aatgatggac aatcggtcat tttcaatttg tctaaactat aagtttcata
    52801 aaaaactgtt aaagggcgaa tattgccata attcagtgag ttcagttgat gaaagaagga
    52861 aaataacatg gaaaatttta aaaaaagcgg aatgttttcc gtaattgctg ctctaggggc
    52921 taatattcta gttgctatca gcaaatttgt cggctatgca atcagtggca gtgccgccat
    52981 gctaaacgaa agcatccaca gtatcgttga ttgcagcaat cagattttgc tgttattcgg
    53041 tgataaacaa gccaataaag gacagagcga gttgcatcaa tttggtgaag gacgagcaaa
    53101 atactttttc agtacaatcg tcgcaatgat gcttttcttc ggcggtgggg cacttggggt
    53161 gatggaagct tttgaaaagt tgacgcatcc atcccatgaa gtgggtaatc cgatcattgt
    53221 tatagcaata ctgttatttg gtttggtaat cgaaggaagt tcgttacgcg tggcaatgaa
    53281 agaaatcaaa gaattgaata ctgaaaaact ttccacgatg cgctttttgc gcgaaagtcg
    53341 acacagtgaa atactgatta tttttacgga agacttgtgt gctgtaatcg gtttggtctt
    53401 ggctttagca ggaacaatat tgacttatgc aaccgggatt gctgcatttg atgcgatcag
    53461 cggattgctg atcggtttct tgctgatggg agccgccatt ttcctagcaa aagaatttta
    53521 tagtctgatc atcggtgaaa gcgtgacagc aagcgattta tcaaaaatca agttagcttt
    53581 tgaacgtcct gagatcagtc ggctgatcga tataaaaaca gttcatttaa gtccaacgga
    53641 gattttagtt gcagcaaaag cagatatcgt tggtaacaaa gagaatgaag cttactcggt
    53701 catcaatgat attgaaaaaa atatgcgtca gtctcttcct gacaagaaga tgtatatata
    53761 cattgaaaca gataaatatg acccaaatta ttctcgtaaa taaaacgttt attcatgaat
    53821 tgaagcaaaa atgagaaaaa tataatcttg tttttaaata aaacggctac aatttagcga
    53881 atttatgtta caatgagtcc gctgtttatt tgttgtaatt aaaggagata ttttaatgaa
    53941 caaaaagaaa agtgcgccat ggcttcaagc tttgcatgca gtgatgccac tttgtataag
    54001 ctatatacca gtaggattag cttgcggagt attgttgcaa aaagttggat ttgatcctct
    54061 cttatcagga ctgttatcga ttctggtctt ttccggtggc gcgcagtttt tagttgcatc
    54121 tatgcttacg acgcaagcat catttgcaac gacattattg atggttttct ttttagaatt
    54181 acgctataca ttattaggag caagtcttgc cggttttatg aaaaaagaaa agcggacgtt
    54241 tttggctgtc ttttctcaat cactgaatga tgaaaactat gcggtgaact acttaaagtt
    54301 ttcaactgac ccttcctgga ataaaagaaa ggctttatat gtcaattggt tctcaatgac
    54361 tgcatgggcc ggtagcaata tgatcggtaa ttttttcggc tcggtgattc gagtagatgc
    54421 tgaccttgtc cattttgctt tgacggccat gttcattttc atgtttatca tgcagatgaa
    54481 aagcgcacta ctgatcttta caggtttatt ctcaggcgta ttaggggtcg tattcatggt
    54541 attattccaa aatactttcg gattgattgc agcaacagtc attgcttcat ttgccggatt
    54601 tatgctggag aaggcattca aaaaagaaaa agctaagaaa ctaagagacc gcggtaaaga
    54661 tgtcaatgag ccgctcttca acttcccgga acaggaggcc aatcaccatg actaaactat
    54721 atttgctact tttagttgct tgtctgttca tcgtggcata tatcccgcgc ttgttcccga
    54781 tgctttattt tactcatcga aaagtgccat cctggttcag tgactggatg aaatatgtac
    54841 cggtggcatt atttgcggcg ttagctttta aagatgtttt cattactcat gaacatctgg
    54901 atattgcttg gaatatcaaa atagctgcaa tgattctagt agcaggcgtg gcttacaaaa
    54961 cacgttcgat ggcgttgtca gttttgacag gacttgcgtc tgtctttttg ctgtctatgc
    55021 tttaaactgt tttattttat taaaaacaga gattgcgaca taagttgttc aaactgatct
    55081 atccgaactg catttgcaac agtcaattct ggatgcaagt ttattcgtag ggataaaaaa
    55141 gattagtgtt ggcttatgat gcaagctctt ttttatttta ggaattagag agggatttgt
    55201 ccttctatga ttttttgcta taatagggaa gatgtttatt ttatgaaaga atagttgagg
    55261 gaatttaaat gaataaaaaa ttgatgcaag gaacattttg gctaaccttt gctaatcttc
    55321 tatgtaaagt tttaggggta gtctatttga ttccctggct aagtatgatg gggaacaatc
    55381 aagatggtat gcttgctacg acattgtata atgttggcta tcttccttat ggattgttcc
    55441 tgatgcttgg tacagtcgga tttccaaatg cgattgcaaa aaaagtagct gtagccacaa
    55501 aagaaggaga taacgctgct tgtcggctga tttttagaag tacgatcaat atcatgtttg
    55561 tcatcggtat cgcctctgct gcactgatgt atttatttgc tcctttacta gccggaatca
    55621 gtccgattgc taacgttaat aatgggatac tagctatcag aagtttatgc ccatctttga
    55681 ttgctatccc aattcttagt gcaatgagag gatatttcca aggaaaaaat gatctgcggc
    55741 cttatggaac ttctttgatt atcgaacagg ctgtgcgagt gatcgttatc ttggctggta
    55801 cttattactt gcgcgtattg actgacggaa ccatattaga agctgtattg atcagtacag
    55861 tcgcttcttt cttcggtggg ttagctgcga ttatccatat gtatatcgtc ggacgcagaa
    55921 aagacttctt cgaacttaaa gattttttga tttccagccg ttatttcgaa agagaaaacc
    55981 gatctgcctc tgtctctatc attcgggaaa cactaccgtt tatttttgtc ggctcagtca
    56041 ttactatcgt tcaaatgatc gaccaagtaa cgatgaaacc actgcttcac tttttccgtc
    56101 cagagatcgc agatcagcaa ctggaattct tattcagccg tgcttctgtc aatccaaata
    56161 aattgacatt gatcctgatc tccatggtag gaacagtagc tataagcagc ttgccaatat
    56221 taagcacgat gaagaaaaaa gatcggatac aaatcgaaaa aacagtagga gatagttttt
    56281 cgattgcgtt attgattctt cttccttcat tgactggaat gtctttatta gctggaccac
    56341 tatacacatt gttttttggc tatgatcctg aaagtgtcgg atatttccaa atggctttgc
    56401 tagcttcttt gtttttctct ttattcacga tcctatctac gatgcttcaa tctttgaatc
    56461 accatcgttt tgcgatccga ttaactgtag aagcaattat cctgaaagtc atcttccaag
    56521 taatcggatt aggattagtt ggcggatacg ggatgagctt atcaaatggc ttagcattcg
    56581 gtattgtctt tgttcgcggc tatcacttta tgtgcaaaga ataccgaata gcacctttag
    56641 caaagatcag caattttttc ttaaaaactt tccgctccac cctgattatg cttgcagtat
    56701 gttttgccgt tttctttttg ctgaatcagc gattgtcaat ggaatcaaaa agctatgcaa
    56761 tgatttattg tgtggctgta ggaagtattg gcggcttagt atttttattt tctcaattcg
    56821 gaaaaaatgg gtttcgaatg ttgaaaaatg ttaaacatca tagcagaagc aaagaatagg
    56881 ttaactaatc gttttttgct gtcacttgaa aaacggcaac tcgtatgatt acgcttgtcg
    56941 tttttctatt ttccaatttt agtttcatat ctaatcttac ttatgatata gttataaggt
    57001 ctggaaaaat tctttgaaac gaggaaatta aacacatgaa acttgcagta gttacagata
    57061 gttcggcata tctgccggaa cgtatcaaga accatcccga cttattcgtt attccaatcc
    57121 ctttgattat ggatgggaaa atttataacg aaggaatcga tatcgaagcg gatgaatatt
    57181 atggactttt aaaaaacagt aaagattttc ctactacttc acaacctgtc gtaggggaag
    57241 ttttgggttt atatgaggaa ttgaaagaga aagggtatga cacgatttta agtatccatc
    57301 tttcttcagg gatttctggc tttgtcaaca cattatttgc gatgaaagat gacatcagcg
    57361 gagtaacggt cattccttat gactctaaga tcactagtat gccgatggga cacatggtag
    57421 aagctgcctt agacttaaat gatgaaggtc agtcccttga tgaaatactt gcacatatcg
    57481 atcgaatccg agataatacc tatgcttatc tgattgtaga tgatttgaac aatttagtac
    57541 gaggaggacg tttgacaaat ggtgcagcgc tgattggcag cttattaaaa atcaaaccga
    57601 tcctgacatt ttctgaaggg aaaattgttt tattcgaaaa aatccgttca agtaaaaaag
    57661 catttgctcg agctgaagaa attattggta gaagaaataa cgaaatacaa ccgccagtca
    57721 aactttacgt gatacatgca aataatttag aagtagcgat tgcggagaaa gagaaattgc
    57781 agaaaaaata tccaaatgca gaaatcgaaa ttggtcattt cggaccagtc attggtacac
    57841 atttaggtga aaaagccatc ggcattgcca tttcagcgca ataattcctt ttattcaaaa
    57901 aacataccaa aaata
//
LOCUS       DAJSBL010000016        56650 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.016, whole genome shotgun sequence.
ACCESSION   DAJSBL010000016 DAJSBL010000000
VERSION     DAJSBL010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 56650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56650
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.016"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            25..1857
                     /gene="tet(M)"
                     /locus_tag="ONT85_001077"
     CDS             25..1857
                     /gene="tet(M)"
                     /locus_tag="ONT85_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HCR2378068.1"
                     /translation="MTELGSVDKGTTRTDNTLLERQRGITIQTGITSFQWENTKVNII
                     DTPGHMDFLAEVYRSLSVLDGAILLISAKDGVQAQTRILFHALRKMGIPTIFFINKID
                     QNGIDLSTVYQDIKEKLSAEIVIKQKVELYPNMCVTNFTESEQWDTVIEGNDDLLEKY
                     TSGKLLEALELEQEESIRFHNCSLFPVYHGSAKNNIGIDNLIEVITNKFYSSTHRGQS
                     ELCGKVFKIEYSEKRQRLAYIRLYSGVLHLRDSVRISEKEKIKITEMYTSINGELCKI
                     DKAYSGEIVILQNEFLKLDSVLGDTKLLPQRKKIENPHPLLQTTVEPSKPEQREMLLD
                     ALLEISDSDPLLRYYVDSTTHEIILSFLGKVQMEVISALLQEKYHVEIEIKEPTVIYM
                     ERPLKKAEYTIHIEVPPNPFWASIGLSVAQLPLGSGVQYESSVSLGYLNQSFQNAVME
                     GIRYGCEQGLYGWNVTDCKICFKYGLYYSPVSTPADFRMLAPIVLEQVLKKAGTELLE
                     PYLSFKIYAPQEYLSRAYNDAPKYCANIVDTQLKNNEVILSGEIPARCIQEYRSDLTF
                     FTNGRSVCLTELKGYHVTTGEPVCQPRRPNSRIDKVRYMFNKIT"
     gene            1976..2143
                     /locus_tag="ONT85_001078"
     CDS             1976..2143
                     /locus_tag="ONT85_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HCR2378069.1"
                     /translation="MCPVCGNKTRLKIREDTELKKFPLYCPKCRQENLIEIKQFKVTV
                     ITEPDAKTQSR"
     gene            complement(2203..2556)
                     /locus_tag="ONT85_001079"
     CDS             complement(2203..2556)
                     /locus_tag="ONT85_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR2378070.1"
                     /translation="MRKKEDKYDFRAFGLAIKEARLKRGLTREQVGALIEIDPRYLTN
                     IENKGQHPSIQVLYDLVSLLHVSVDEFFLPANNLVKSTRRLQIEKYMDSFTDKELSLM
                     ESLASGINEARNIED"
     gene            3061..3384
                     /locus_tag="ONT85_001080"
     CDS             3061..3384
                     /locus_tag="ONT85_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002414693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCR2378071.1"
                     /translation="MKPSSFQTTIENQFDYICKRAMEDERKNYMLYLSRIAKREVSFS
                     DVGDYLVSQFATTDNYSTDFQIFTLNGLSVGVENDLLSEALRELPDKKREILLLFYLW
                     EFRYF"
     gene            3363..3692
                     /locus_tag="ONT85_001081"
     CDS             3363..3692
                     /locus_tag="ONT85_001081"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2378072.1"
                     /translation="MGISLFLELPAIVSYWVVTVMCFVAMGVSTMFVVQIYTLVQTQT
                     PPQLVGKIMATLVAVAMCGQPIGQMVYGILFDVFIQETWVVMFVASIASIMISIYSKR
                     IFQKLEI"
     gene            3919..4338
                     /locus_tag="ONT85_001082"
     CDS             3919..4338
                     /locus_tag="ONT85_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009588541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HCR2378073.1"
                     /translation="MRTSSFEHIVRLQFNSLMLIVIKNKVKSRNRQLARHSKREVLFC
                     EIAETKQVERGTIDTCFYDCVSFKVLHFTIYVSDETLGTVLHRLSEKQRSAILLRYFQ
                     GMNDRKISELYHVSRSAICSRRTRGLRKLKMLLSEGK"
     gene            4343..4582
                     /locus_tag="ONT85_001083"
     CDS             4343..4582
                     /locus_tag="ONT85_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004609551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR2378074.1"
                     /translation="MSKDYGYPSFELICKASSGDEMAVKEILKFYDGYISKLCLRPFY
                     HSESGKIIMQVDEELKGEIHTDMMKAILKFEIGVK"
     gene            4840..5685
                     /locus_tag="ONT85_001084"
     CDS             4840..5685
                     /locus_tag="ONT85_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCR2378075.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIEKDA
                     ISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESI
                     YNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFE
                     RILTI"
     gene            5966..7186
                     /locus_tag="ONT85_001085"
     CDS             5966..7186
                     /locus_tag="ONT85_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HCR2378076.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            7183..9903
                     /locus_tag="ONT85_001086"
     CDS             7183..9903
                     /locus_tag="ONT85_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR2378077.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            9887..10831
                     /locus_tag="ONT85_001087"
     CDS             9887..10831
                     /locus_tag="ONT85_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HCR2378078.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(10900..11910)
                     /locus_tag="ONT85_001088"
     CDS             complement(10900..11910)
                     /locus_tag="ONT85_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCR2378079.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(12090..12434)
                     /locus_tag="ONT85_001089"
     CDS             complement(12090..12434)
                     /locus_tag="ONT85_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HCR2378080.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            complement(12427..12861)
                     /locus_tag="ONT85_001090"
     CDS             complement(12427..12861)
                     /locus_tag="ONT85_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HCR2378081.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            12999..13736
                     /locus_tag="ONT85_001091"
     CDS             12999..13736
                     /locus_tag="ONT85_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR2378082.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            13729..14940
                     /locus_tag="ONT85_001092"
     CDS             13729..14940
                     /locus_tag="ONT85_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR2378083.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            15144..15926
                     /locus_tag="ONT85_001093"
     CDS             15144..15926
                     /locus_tag="ONT85_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HCR2378084.1"
                     /translation="MMEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAA
                     LSREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLK
                     TMLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHG
                     DVNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDE
                     MLEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            15996..16658
                     /gene="trmB"
                     /locus_tag="ONT85_001094"
     CDS             15996..16658
                     /gene="trmB"
                     /locus_tag="ONT85_001094"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HCR2378085.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(16756..18510)
                     /gene="ade"
                     /locus_tag="ONT85_001095"
     CDS             complement(16756..18510)
                     /gene="ade"
                     /locus_tag="ONT85_001095"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HCR2378086.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            18642..19571
                     /gene="tadA"
                     /locus_tag="ONT85_001096"
     CDS             18642..19571
                     /gene="tadA"
                     /locus_tag="ONT85_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component
                     TadA"
                     /protein_id="HCR2378087.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGTVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLKLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            19528..20577
                     /locus_tag="ONT85_001097"
     CDS             19528..20577
                     /locus_tag="ONT85_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGB"
                     /protein_id="HCR2378088.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            20574..20852
                     /locus_tag="ONT85_001098"
     CDS             20574..20852
                     /locus_tag="ONT85_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HCR2378089.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            20827..21297
                     /locus_tag="ONT85_001099"
     CDS             20827..21297
                     /locus_tag="ONT85_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HCR2378090.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            21314..21616
                     /locus_tag="ONT85_001100"
     CDS             21314..21616
                     /locus_tag="ONT85_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2378091.1"
                     /translation="MESLTALMIASVCVYLVSFGQLFLLQAEKKSGEELLFIRMIYED
                     IKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAISIEKTSNSSIYAD"
     gene            21633..22004
                     /locus_tag="ONT85_001101"
     CDS             21633..22004
                     /locus_tag="ONT85_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComGF family competence protein"
                     /protein_id="HCR2378092.1"
                     /translation="MILSSLLLTFNLLIQQTIQVSEFLQRKDQKEWLIFLAQLEEELK
                     SSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQPMLTSITELQLLEKEHTI
                     SFYVHFENGEDGYAIWTKNDQ"
     gene            21976..22335
                     /locus_tag="ONT85_001102"
     CDS             21976..22335
                     /locus_tag="ONT85_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2378093.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            22507..23514
                     /locus_tag="ONT85_001103"
     CDS             22507..23514
                     /locus_tag="ONT85_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCR2378094.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            23539..24723
                     /locus_tag="ONT85_001104"
     CDS             23539..24723
                     /locus_tag="ONT85_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HCR2378095.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(25160..26743)
                     /locus_tag="ONT85_001105"
     CDS             complement(25160..26743)
                     /locus_tag="ONT85_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HCR2378096.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVDINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHCQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(27321..27464)
                     /locus_tag="ONT85_001106"
     CDS             complement(27321..27464)
                     /locus_tag="ONT85_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HCR2378097.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(27686..28165)
                     /locus_tag="ONT85_001107"
     CDS             complement(27686..28165)
                     /locus_tag="ONT85_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HCR2378098.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(28238..28522)
                     /locus_tag="ONT85_001108"
     CDS             complement(28238..28522)
                     /locus_tag="ONT85_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2378099.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(28600..28794)
                     /gene="ssrS"
                     /locus_tag="ONT85_001109"
     ncRNA           complement(28600..28794)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ONT85_001109"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(28866..30152)
                     /locus_tag="ONT85_001110"
     CDS             complement(28866..30152)
                     /locus_tag="ONT85_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HCR2378100.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            30290..30961
                     /locus_tag="ONT85_001111"
     CDS             30290..30961
                     /locus_tag="ONT85_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HCR2378101.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            30927..31415
                     /locus_tag="ONT85_001112"
     CDS             30927..31415
                     /locus_tag="ONT85_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HCR2378102.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            31428..32375
                     /gene="prmA"
                     /locus_tag="ONT85_001113"
     CDS             31428..32375
                     /gene="prmA"
                     /locus_tag="ONT85_001113"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HCR2378103.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            32376..33140
                     /locus_tag="ONT85_001114"
     CDS             32376..33140
                     /locus_tag="ONT85_001114"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HCR2378104.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            33373..35586
                     /locus_tag="ONT85_001115"
     CDS             33373..35586
                     /locus_tag="ONT85_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HCR2378105.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            35597..36043
                     /locus_tag="ONT85_001116"
     CDS             35597..36043
                     /locus_tag="ONT85_001116"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HCR2378106.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            36214..37473
                     /locus_tag="ONT85_001117"
     CDS             36214..37473
                     /locus_tag="ONT85_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HCR2378107.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(37562..38509)
                     /locus_tag="ONT85_001118"
     CDS             complement(37562..38509)
                     /locus_tag="ONT85_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HCR2378108.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            38903..40195
                     /locus_tag="ONT85_001119"
     CDS             38903..40195
                     /locus_tag="ONT85_001119"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HCR2378109.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            40210..41979
                     /gene="aspS"
                     /locus_tag="ONT85_001120"
     CDS             40210..41979
                     /gene="aspS"
                     /locus_tag="ONT85_001120"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HCR2378110.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            42088..42963
                     /locus_tag="ONT85_001121"
     CDS             42088..42963
                     /locus_tag="ONT85_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HCR2378111.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            43229..45112
                     /locus_tag="ONT85_001122"
     CDS             43229..45112
                     /locus_tag="ONT85_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HCR2378112.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(45187..45471)
                     /locus_tag="ONT85_001123"
     CDS             complement(45187..45471)
                     /locus_tag="ONT85_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HCR2378113.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(45461..46195)
                     /locus_tag="ONT85_001124"
     CDS             complement(45461..46195)
                     /locus_tag="ONT85_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HCR2378114.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            46324..46947
                     /locus_tag="ONT85_001125"
     CDS             46324..46947
                     /locus_tag="ONT85_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HCR2378115.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            47139..48260
                     /locus_tag="ONT85_001126"
     CDS             47139..48260
                     /locus_tag="ONT85_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HCR2378116.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            48267..51395
                     /locus_tag="ONT85_001127"
     CDS             48267..51395
                     /locus_tag="ONT85_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HCR2378117.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANERIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            51414..54608
                     /locus_tag="ONT85_001128"
     CDS             51414..54608
                     /locus_tag="ONT85_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HCR2378118.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(54754..55461)
                     /locus_tag="ONT85_001129"
     CDS             complement(54754..55461)
                     /locus_tag="ONT85_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HCR2378119.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
     gene            56226..56624
                     /gene="spxA"
                     /locus_tag="ONT85_001130"
     CDS             56226..56624
                     /gene="spxA"
                     /locus_tag="ONT85_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HCR2378120.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT        18623 a         9756 c        11700 g        16571 t
ORIGIN      
        1 agcttattat ataacagtgg agcgattaca gaattaggaa gcgtggacaa aggtacaacg
       61 aggacggata atacgctttt agaacgtcag agaggaatta caattcagac aggaataacc
      121 tcttttcagt gggaaaatac gaaggtgaac atcatagaca cgccaggaca tatggatttc
      181 ttagcagaag tatatcgttc attatcagtt ttagatgggg caattctact gatttctgca
      241 aaagatggcg tacaagcaca aactcgtata ttatttcatg cacttaggaa aatggggatt
      301 cccacaatct tttttatcaa taagattgac caaaatggaa ttgatttatc aacggtttat
      361 caggatatta aagagaaact ttctgccgaa attgtaatca aacagaaggt agaactgtat
      421 cctaatatgt gtgtgacgaa ctttaccgaa tctgaacaat gggatacggt aatagaggga
      481 aacgatgacc ttttagagaa atatacgtct gggaaattat tggaagcatt agaactcgaa
      541 caagaggaaa gcataagatt tcataattgt tccctgttcc ctgtttatca cggaagtgca
      601 aaaaacaata tagggattga taaccttata gaagtgatta cgaataaatt ttattcatca
      661 acacatcgag gtcagtctga actttgcgga aaagttttca aaattgagta ttcggaaaaa
      721 agacagcgtc ttgcatatat acgtctttat agtggcgtac tgcatttgcg agattcggtt
      781 agaatatcgg aaaaggaaaa aataaaaatt acagaaatgt atacttcaat aaatggtgaa
      841 ttatgtaaaa tcgataaggc ttattccggg gaaattgtta ttttgcaaaa tgagtttttg
      901 aagttagata gtgttcttgg agatacaaaa ctattgccac agagaaaaaa gattgaaaat
      961 ccgcaccctc tactacaaac aactgttgaa ccgagtaaac ctgaacagag agaaatgttg
     1021 cttgatgccc ttttggaaat ctcagatagt gatccgcttc tacgatatta cgtggattct
     1081 acgacacatg aaattatact ttctttctta gggaaagtac aaatggaagt gattagtgca
     1141 ctgttgcaag aaaagtatca tgtggagata gaaataaaag agcctacagt catttatatg
     1201 gaaagaccgt taaaaaaagc agagtatacc attcacatcg aagttccacc gaatcctttc
     1261 tgggcttcca ttggtctatc tgtagcacag cttccattag ggagcggagt acagtatgag
     1321 agctcggttt ctcttggata cttaaatcaa tcgtttcaaa atgcagttat ggaggggata
     1381 cgctatggct gtgaacaagg attgtatggt tggaatgtga cggactgtaa aatctgtttt
     1441 aagtatggct tatactatag ccctgttagt accccagcag attttcggat gcttgctcct
     1501 attgtattgg aacaagtctt aaaaaaagct ggaacagaat tgttagagcc atatcttagt
     1561 tttaaaattt atgcgccaca ggaatatctt tcacgagcat acaacgatgc tcctaaatat
     1621 tgtgcgaaca tcgtagacac tcaattgaaa aataatgagg tcattcttag tggagaaatc
     1681 cctgctcggt gtattcaaga atatcgtagt gatttaactt tctttacaaa tggacgtagt
     1741 gtttgtttaa cagagttaaa agggtaccat gttactaccg gtgaacctgt ttgccagccc
     1801 cgtcgtccaa atagtcggat agataaagta cgatatatgt tcaataaaat aacttagtgt
     1861 attttatgtt gttatataaa tatggtttct tgttaaataa gatgaaatat tttttaataa
     1921 agatttgaat taaagtgtaa aggaggagat agttattata aactacaagt ggatattgtg
     1981 tcctgtatgt ggaaataaaa cacgattaaa gataagggaa gatactgaat taaaaaaatt
     2041 ccccctctat tgtccgaaat gcagacaaga aaatttaatt gaaataaagc agttcaaagt
     2101 aactgtgatt acagagccag acgcaaagac gcagagccga taaaatgaga ttaatacaat
     2161 ctcattttat cggctctttc cgttatgtat ggattctttt aattagtctt cgatgtttct
     2221 tgcttcgttg ataccgctgg ctaaagattc cattaaggat agttctttgt ctgtaaagct
     2281 atccatgtat ttctctatct gtaatcgtcg ggtgcttttt accaagttat tagcaggtaa
     2341 gaaaaattca tcaacggaaa catgaagtaa cgatacaagg tcataaagaa cttgtatgct
     2401 ggggtgttgc cctttatttt caatattagt taagtaccgt gggtcaattt caatcaatgc
     2461 tcccacttgt tcacgagtta aacctcgttt caatcgagct tctttaatgg ctaaaccaaa
     2521 ggctctaaaa tcatatttat cttctttttt acgcatagta gaccacctct atacatttta
     2581 ttgttcctac tgaattaaaa acaggtatag aaaaacgtgt tatatggttt ataggtttat
     2641 atttaataaa aagcactact aaacgccaat aaaaaaaacc gttatatggt agtgctattt
     2701 acgctgttaa aatattgtat attacttcca aatggcggtt tgttggaggt caacgtcgcc
     2761 atgaagtaca tcatatacaa taaatttcct tacattgggt tcttgtcaaa aaaagtcgtc
     2821 tatctgcaat agataagtac gtccaccaat gtggttttat aaatcatata gatagaataa
     2881 cagaagcatg taaacagaga aataaatctg tttatatgct tttttggcta ttcagaactt
     2941 ttttacaaag tttatttatc agtaatgcaa caaatccccc tttcacattg ggactaagag
     3001 tgaaaggaga taaacgagca aggctcactt cctttcctag acagaaaggg ggtgagaaac
     3061 atgaaaccat cttcttttca gaccacaata gaaaatcagt ttgactatat ctgtaaacgt
     3121 gctatggaag acgagcgaaa gaattatatg ctttatcttt caaggattgc aaagcgtgag
     3181 gtgtcctttt cggatgttgg cgattatctt gttagccagt ttgcgacaac agataactat
     3241 tcaactgact ttcagatttt tacactcaat gggttatcag taggcgttga aaatgatttg
     3301 ttgagtgaag cattacgtga gttgccagac aagaaacgtg aaattctact gctgttttac
     3361 ttatgggaat ttcgctattt ttagaattgc ctgcaatagt ttcctactgg gtagttacag
     3421 tgatgtgctt tgtggctatg ggtgtatcta ctatgtttgt tgttcaaatt tacactcttg
     3481 ttcagacaca aacaccaccg caattagtag gaaagataat ggctacgctg gtagcagttg
     3541 ctatgtgcgg tcaacctatt ggacaaatgg tttacggtat tttattcgat gtatttatac
     3601 aagaaacatg ggttgttatg tttgtagctt ctattgcttc aatcatgatt tcaatatatt
     3661 caaaaagaat ttttcaaaag ttagagatat aatacaaggg gttaaatagt atgttaggct
     3721 aaaaacaata tataaaagaa aatttgaaat ctgctaaaag gatagtgtta taaaagaaag
     3781 agtaatattg cagtatttaa ggctcgcaca aatatatgtt ttgtgtgggc ttttttctta
     3841 ccatttttct gtatttctaa agggtataaa cggtattccg tttatatatt tcagcattag
     3901 cagaaagggg gtgagattat gaggacatct tctttcgagc atatcgtcag attacagttc
     3961 aattctttaa tgctgattgt cattaagaac aaagtgaaaa gtcgaaacag acagcttgca
     4021 agacattcta aacgtgaagt attgttttgt gaaatagccg aaacgaaaca agttgaacgt
     4081 ggaacgattg atacctgttt ttatgattgt gtatcattta aagtcttgca ctttacaatt
     4141 tatgtatctg atgaaacact tggtacagtt ttacatagac tgtcagaaaa acagcgtagt
     4201 gcgattttat tacgttattt tcaaggaatg aacgacagaa aaatttcaga actgtaccat
     4261 gtatcacgtt ctgctatttg cagtagaaga actagaggac taaggaagtt aaaaatgctt
     4321 ttgagcgaag ggaagtaaag aaatgagtaa agattatggc tatccctctt ttgaattgat
     4381 ttgcaaagcg tcaagcggag atgaaatggc agttaaagag attttgaagt tttatgacgg
     4441 ttatatttct aaattatgct tgcgtccttt ttaccactct gaaagtggta aaatcattat
     4501 gcaggttgat gaagaactaa aaggagaaat tcatacagac atgatgaaag caatattgaa
     4561 atttgaaatc ggagtgaagt aatcaactgt ataaaaaaat cgggagatac acagtttcgt
     4621 gctatctccc ttagtagttt tacggtcata tcaaggctca ttcaatcgtg gtaaattcgt
     4681 ggtaagatga gtgtttttct atacttcaaa atgcttgaat gtatagtgtt tatgcggata
     4741 attgaaaatt agatataaaa tttcttacaa ttagtatatg ttttgtgaga aatttagact
     4801 tacaataagc agtgaattta taatcaacgt ataggagaca tggatgaaat gaaactaatt
     4861 ggtatagatt tagacggtac tcttttgaat tctgaccaga gcattagtca aaaaaatgca
     4921 agaacaatga aacatttacc ggaagactgt tttccattca tttgttctgg aagagaagta
     4981 gaagacatta ataatattct caaaaaatct ggtttaagga taccagcagt agggttaaat
     5041 ggttctgttg gatttgatgg agaaaaaaag atatttgagt cttccttcga taaaaatgat
     5101 gtaaaaaaaa ttgattctgt cctatcgaaa tttccaacca aaatatatac aaatctcgga
     5161 agttatgaat ctcagaatta taaaaataaa atgaaaaaaa tttttcacga ggtagggagt
     5221 gaattttcta tcaaagagct aaactatgag cttgagtatg aaaaatctat ttgttcaatc
     5281 ccttataata atattgagga aattatagaa aaggatgcta tcagcattta caaatttttt
     5341 atttttattc ctaatcgtca attgaaagct aatatattta accatttgaa aaagatgcta
     5401 aatgtgtctg ttactgaatc ggcacctgta aatattgaaa tcgtacctca aaatgtatct
     5461 aaaggtgtgg tgtttgatcg tttggaatca atctacaatt taaagaaacc tttgagaatc
     5521 gcgatcggag atagtttaaa tgacttatct atgtttgaaa gtgcagatat tagttttgca
     5581 atgggaaata gtcatcaaga aatcatgaaa aaagctgatt atgtcactgt ttcaaatgat
     5641 aaagacggag tatctcaagc atttgaaaga attcttacca tataaactaa ttaaggtagt
     5701 atagaacata gaattctaaa tacggaaaaa tggagcataa attccagtac aaatagtact
     5761 tcttcagtgt caaacgaagc gaatataaca tagatattta ggattgtgac aaaaatcatg
     5821 tcacaatctt tttttcgaat aaacttattt tttgaagtcg gccatttatc gtaatttctt
     5881 tgcataatac gaacaaattt tcggttattg atgaaaaaaa acattgaaaa tggtatgata
     5941 aaagaaaaaa aggaggaaat ttttaatggt gaaatttatc catgcggcag atttacatat
     6001 ggatcggtca tttgaaggac tgacaactct tgataaaact gtacaggaaa aactattgaa
     6061 gacgaatctg accgttttat caaatatcat tgaacaggca ataataaata acgttgattt
     6121 tgttttactt gcaggagata attttcatca aaatcgtcct tcattaaaaa tacagaaaca
     6181 tttttcagaa cagatgaaac ggttagaaaa gaaccatata cctgtattta taatattcgg
     6241 aaatcatgat ttctatcaaa aagagcggta ttggttctcc tttcctaaaa atgtccattt
     6301 atttacatcc gaaacggtag agacaaaaaa aataacgata aaaagtggag aaactgtctc
     6361 tctcagtgga tttagttatc gtcaaccgtg gattcaaaaa gacaaagtga tggaatttcc
     6421 ttctagagaa ctcacagatt accatattgg gttgtaccac ggggaacctg gcgtctctca
     6481 aaaaggaaac tatgctcctt tccaaccctc aaagatgcaa gaaaagggat acgattattg
     6541 ggctttaggg catatccatg tgccaacggt cttaaatgaa aagcaaacaa ttgtttatcc
     6601 aggtgcgcct caaggtcata ctaaaaagga acaagcttct acatctattc tactagttga
     6661 attaagtaaa ggaagttgcc aaatacatcc gattaaagta gcagaggtct attggaagac
     6721 aaaggaaata tcattgagga aagcaagaac gacaaaagag gtcattgttc acattcgtca
     6781 gcagctttca aaagtagagg atggatttag tttaattgag ataaaattaa aagattatga
     6841 ccatctcaat acagatgttt tggaacggat acaatcagga gagctattag actatttgtt
     6901 agaggaattc tcagatagga taaatgattt ctttatttgg cgtatttctc tcatagagaa
     6961 tactttttat acgaagaccc cacttgctgc ttctgctaaa ttgatggaac agttatttca
     7021 atactatcag acacctcaag atttccaaca aattttaaat gaagtatata gtcatcaaga
     7081 agcggctaga atattgagcg aactgccgga atatcaagaa gaaacacttg aaaaagccaa
     7141 gcagctgctt aatcaagatt ttttatttga ggaggatcag gaatgaagat tttaaggatc
     7201 gagattgctg cttttggaaa atggcgtcaa aaaagttttg acttttattc aggcaatcag
     7261 ctgatctatg ggggaaacga agcaggaaaa tcaacgattt atcaatttat tcaagcaata
     7321 ttatttggtt tcccttcaaa aggccgtaaa aaaaaggatt atacaccaaa agacggctca
     7381 gcctatggcg gcaaaatatg gcttaaacat cctgtatatg gtgaatttgc tgtcgagcgc
     7441 tacaagcagc aaaatcgcgg aaaatcgaag gtgtggctgg gggatcaagt gggaagtgat
     7501 gagttactag aaaaactgat cgcacctttg acgaaagagt tattccagca ggtatttact
     7561 ttccagcaag agcagttgat ggaactagat catttgagag aaaaagagct acatgatgcg
     7621 ctgatttctt taggcatcac gggcagttct caagtattcc aaaagcggca agaatattat
     7681 cagaaagcac aacaattatt caagaaaaaa ggccaaaaat tactaatcaa tcaaaaatta
     7741 aatgaatgga aagagctgca agaaaaaatt catcaaaaac aagaacagga agcttatttt
     7801 ggcaagttga tccaacaaga acaggaatac gagtcgcaac agcagcagtt attggcacaa
     7861 ttaaaagagg cgaacaaaca attttcatta gccagacagc aagaacttaa tttttcttta
     7921 tatgaagaat ggcaatctct caaaaaaaac agcactacag gtcttcctga tgagcaactt
     7981 cttttagatc tggagtcttt ctccaaacgg tatcagcaaa caaatgagcg tttggaagaa
     8041 ttagaagcaa cactggaaaa gcatagcggg atggatcagc agtcagccag atattatttt
     8101 tatttagagc aggagcagga actaaaagat ctccagcaac ttcaatttac agcggaacaa
     8161 ttaaacgaag aatggcaaag attacaaatt gagactagag aaacaaattc agaattgaag
     8221 attttggaac aaaggtggca ttggcagaaa aatccgttac cggaagaatt tttatatgaa
     8281 gaagaatgga agcatcttct ggctgaagaa agaaggattg ctgatctaga aagccagatt
     8341 tctgtgcgtc tccaaatagc taaagaacag caagagatga ttgaagcaga agtgaatgac
     8401 ttggaagaga cctatccagc attattacaa aatcagtcag agatagcacc agtctcttca
     8461 tctaaaccgt atatgctgat ggctggcgga gcaataatca ttgcaggtat atttgtacca
     8521 atgcccttga aagtgatcct tttactaatt ggtgcaggca tgattggcta tttcttctac
     8581 aaaaggcaac caaaaattaa aactaaaaat gatttctctg agataaaagg aatgtggcaa
     8641 gaaaaactag gacaacttga tctagcctta gctcaattag ctgaagcaga agaaaaacaa
     8701 aagagaactg gagaagaaaa acagattctt cagcaagaag tatatcgttt tgcggatcat
     8761 cggcacttag gaaagatgga tacgctcaaa ctcatgaaag aatatggaca ggaagtcata
     8821 gattatcaga aattatatgg cgtatttgag cggaaacata aaagagaaca ggaaatacaa
     8881 gaaaaactac gacaaatcga ccaaaaattt gagtttgtta aagaatggtt accgatacaa
     8941 gataagacaa tttcagaaaa aatggtattg ctttcccgtt ttatacaaga gatggaacag
     9001 atgaaatttg ccagaagcta tcagcaaaat actttattga agcaagaaat caatcaattg
     9061 aaaaagaaac aaaaagtatg gatacaagaa tacagcgaac aattgactca agcaggctta
     9121 tcttatcctt cggaagtacc tgtcttttta caaaaagcca aacaagccga acagcagatg
     9181 aaaagataca atgaattatc ggaaatgctt actccactat ttcctgaaga gctgacaaaa
     9241 gagaaattat ctgtaagact gcgccaactg aaagaaaagc aatcccaact ccagcaagaa
     9301 gaaaaagaag caagtgaaaa tcggcaacga ctccaacttc aagtagaaga gttgcaaaga
     9361 gacggcacat tggacgaact ttatcaacaa gctagccggc aaaaagcaaa attacaggaa
     9421 ttactggagg aatggggagc acttgaaatc gccgggacat tcttaggtga tctttcaaca
     9481 gagatgagtg aaagacaatt accccagttg ttaagagcag caggtgatta tttaggtatt
     9541 ttaacaaaag gaaattatca gaagatatta ctgaaaaatg atacactgac tttaacagac
     9601 ggatatacag aatttcccat ctatgagtta tctacaggta cgaaagatca gctgattatg
     9661 gcgatgagat ttgcttacct ctcattagaa gctaaccgtt cgatatgccc gatcatcatt
     9721 gatgatggct ggcttcatta tgatcatcag cgaaagtatc aattagctga acttttagct
     9781 aaatttggag aaaagtatca aataatttgt ttttcttctg ataaagaaat ggtaagctat
     9841 taccatgaga tgaaccaaat cgtaaaacaa ttagaaggag caaataatga aaaaaattcg
     9901 tgatttagta atcgatgagc agtttgaaca atttgtcctg atcaaaagtg cagatgtaag
     9961 aatcgctaaa aacggaaaaa aatttattgc ttttactttc caagatactt caggtactat
    10021 cgatggtaaa tactgggatg cttcggagga ggaaatcaat aaatttacag ccggcacagt
    10081 cgtttttcta aacggtaaaa gagaggttta tcaaggaaac cctcaagtaa agattcttca
    10141 tatgcgctta acaagggtag atgagccagg agagcctagt ttatacatgg aaagagcccc
    10201 tttgaagaaa gaagaaatgg aagaagaaat caatcaattg ttgtttgaga tcaccaatgc
    10261 gcattggaat cgtattgttc gctatctact taataaatat caaaaacaat tttttgagtt
    10321 tccggcagca aaaagaaacc atcatgcatt tgccagtgga ttagcttatc atactttgac
    10381 aatgcttcac ttagggaaat caatcgccga tgaatatcct gagctgaatc gagcattgtt
    10441 gtatgctggt atcattttgc atgatttagg aaaggtgatt gaactttccg gtcctatgtc
    10501 gacagaatat acgcttgcag gaaatttgat cggtcatctg gtattgattg acgaagaaat
    10561 aactaaagcc tgtcttgcgt tgaaaatcag cgaagctgac gaagatgttc ttgtattacg
    10621 tcatatggtt ttagcacacc atggcttatt agaatatggc tcacctgtac gtccaagaat
    10681 aatggaagca gaaatattac atcaaatcga caatatcgat gcttcgatgc agatgatgtt
    10741 gacttcgatt cgtcaaacag aaccaggaaa atacaccgat cggattttcg gtatggacaa
    10801 ccgaagtttc tatgtaccaa aagatttata gcaaaaattt atttttttca ttcaaaacaa
    10861 aaaaagcgag acataagtct tcgctttttt tgttttcttt tattttgatg aatcagttgt
    10921 ttcttcagtt gaagattctt ttgttgaatc agttgatttt gtgtcagaac tctttgttga
    10981 agctgtagtt tcacttgagt ctttcgtact tgatgaagtt gttgtgaatg cactcaacac
    11041 attttcaaag gcatcatctt tgatttttac atttgcatct tttaattctt caccgattac
    11101 tttagttgtg aatgcattgt cacttagttt agtttcagta gcgatatctt ttagttgatc
    11161 tttgtattta tccatgtcat tgcctttatt ttggtttttc accattttga caacgtagta
    11221 ttctgtcgca taagaagtag gatttgttgt agtaataaca tcggagatct caccgtcttt
    11281 caatttgaaa gctgcttctt ttacttctgc aggaatagtt gttgtcgttg agtcgaattt
    11341 aactttaccg ccatcttctt ttgtctctgt atcagttgat ttgtctttgg ctaatttaga
    11401 gaaatcatct ccatcatccg ctgatttttt cacatctttt gcttcatctt cacttgataa
    11461 tttgatgatt tgtgcttcta cttctggatg gaaagatttc caagcagttt tcaaatcgtc
    11521 atctgtaata tctacgtgag ctttcaagcc agcttcaaaa gctaaattgt tacgaatata
    11581 ttctttataa gtatctttcg tatatccagc agcttctaat tgtgattcaa aagtatcacc
    11641 taatgatttc gcttgtttgt cgtattctgc atccacttgc ttatcggtta ctttatcgcc
    11701 atatttgtta ttgaatactt tataaatgat catccgttgt actaaggatt ggttcgatga
    11761 ttcaagtttt gcttcatcat agaaatcaga tacagtgatc gttccgcctt tcatcgttgc
    11821 gatatcttta ttagtatcgc ctgaacaagc tgctagagtt aaaacagcca acgcactagt
    11881 agctgccaaa ataattgatt tttttttcat taaaatttgc actcctgtaa ttagttatta
    11941 tttttatata ttaactatac catatcccat ttcctttaaa aacgttgtcg tggtagtttc
    12001 aaacaaaatt caaaatttca tcacattcct aggaaatacg caaacttttc tcacttatat
    12061 agttaaatct gttgagaaaa taatcattat tatatttgtt caggatactc tggcagatca
    12121 gcttttagtt tttctacttg ttcattgatt tgtgtgatac gtggttcggc ttggaattta
    12181 aaatcttcga tatctttttt gattgcttct tggaaagcag gaatcacaga ttcagctgtt
    12241 tcttttgttt cgatcaatgc gcttttaaaa tgattcaggc tgttatttaa atcatttgtc
    12301 agagttgttg cttcttccaa ttcttcgatc agtttctttc ttgtttcatt cccgctcctg
    12361 ggcgccaata acagacctcc gacagtgcca gcagcagctc caaataacag tcctttgaaa
    12421 aagtttttaa tcatcgcagg acacctgctt tacgattgtt tcttgaatag cttttagttc
    12481 ttccgatcca taactatctt gatgattgac aaagtgaata gaaaaatcat cctctttgct
    12541 gtagcgaggg actaaatgga tatgtgaatg aaaaacagat tgataggcta gttcacggtt
    12601 gttattcaag atattaagac ctttcatttc tggaaaagct ttttccaatg cttgagcaat
    12661 cttaggcaca cgcgaaaaaa cctctcctgc aagttcggga tcatattcaa aaatatctgc
    12721 tacatgacgt ttaggcacaa ctaatgtgtg tcctttcgtt gcttgagaga tgtctaaaaa
    12781 agcatagacc ttgtcgtctt catatatttt atagcttggg atttcttgat tgatgatttt
    12841 gcaaaaaata cagttttcca tataaaacat cctttctata aagaataaat acttcagctt
    12901 aactttacca taagttaaag ctattgtaag gaaaaaaaca aaataccgtc attttcttcg
    12961 tggtataata aataaaaata aatggcggag gaaaaagaat gagtttaatt atcgatcatg
    13021 tcacaggggg gtacggacat ctccctgtgt taaaagatat cagtttcgat gtacagcctg
    13081 gagagatagt agggttgatt ggactgaacg gagcagggaa aagtacgacc atcaagcaca
    13141 ttatcggtct gctggaagct tcaaaaggaa cgatagaaat caatcatcta tccttaaaga
    13201 aaaatacgga agaatatcgc aaacaaattg ggtatattcc agagactcct tctttatatg
    13261 aagaattgac attgagagaa catatcgaag tcactgcgat ggcctacgat atacctatgg
    13321 atatagcaat gaaaagagca gaaaagttgt tggagacttt tcggttatct aatcgtcttg
    13381 actggttccc agctaatttt tcaaagggaa tgaaacagaa agtgatgatc ttatgcgcct
    13441 ttttgatcga gccttcttta tatatcatcg acgaaccatt tttaggcttg gatccactag
    13501 ctatcaatgc attgcttgaa ttgatggtag aaatgaaaaa agaaggagcc ggtattttga
    13561 tgtctacgca tattttagct actgcagaaa aatattgtga tcgttttgtg gtcttacata
    13621 acggagagat acgagcagac ggttctcttg agatgttgag aaaagaattt cagctgccgg
    13681 attcttcttt ggacgaaatc tatattgctt taacaaagga gcaggcacat gagtagtttt
    13741 tttcaaaaac gtttagcaag acatcaaaag aaaatgatga aatatatgcg ttatgtattg
    13801 aacgatcatt ttgttttagt ttgtcttttc ttgattggcg gtgtgggatt atattattct
    13861 aattggttga aaaccctgca gccaccgttt gcaattggag ggctcataat ctctgtattt
    13921 tggatgctct gtttattcgt gggtaagatg gctacttttg cagaggcagc agatattgtt
    13981 tttttacttc ctaaagaaaa agaaatgcaa acctatttgg aacgagcttt tcgctattct
    14041 tgcatttttc cgttgatctt tttgatattg gcatgcggat tggccatgcc attagtggtg
    14101 gttagtacgg agcaatcctt ttcgatgttt ttcgtctatc tgatcatatt atggagtttg
    14161 aaattcagtc atctgcaaat caagagagca gaattgtttc gactagatca acaagttatt
    14221 cgttattgga aattaggctg gtttttttct tctctgatct ttttgtttct atcattttat
    14281 tggctaatat gggcaggaat agcaggtgca ttagtacagg caagtctata ttactggttt
    14341 ttgtggaagc aacaagaaaa aggattggat tgggagaaaa tggtgcatgt agaagaacat
    14401 aggttgcatc agatctatca atttatcaat ttattcacag atgttcctga aattacggca
    14461 aagatcaaaa gaagacgcta tttggatcct atattgactc atattaaaag agaatcaaaa
    14521 aacacttatc tttacttgtt tgcaagaaga ttggcaagag gttctgattt tagtggtctt
    14581 tatttccgat tagtaattat aggtgggtta ttgattgccg gagtagtcga cttttatttt
    14641 agtctgatta tcggtttctt atttttatat ttgatcggtt tccaactgtt acctttagcg
    14701 aaccagttcc gctatatggt tctcgttcag ctttatccaa tccataagaa tcagaagaag
    14761 caggcgattc agtatctact cacatgggta ttaattatcg cgtcggttat ttttggtttg
    14821 attgctgcag tcgtattaga tgggcagcag cgatgggttc cgctagcggc ttatttgatt
    14881 gttactatgc tctttgttaa actatatgta ccaactcgtt tgaagaagat gtcagactga
    14941 aaacaaagct gtgtagggta aaaatgagaa tgaaaaatag agcgttgaca gtaagaatgc
    15001 tgttcgttct attttttttg agaaaagctg gttttttgaa tggcaattaa atgaaaaaga
    15061 acgtatatgt gcttgacgtg tagtgttcag gcgactagaa taataagtac tgcaaaacaa
    15121 agagtaagag gacgggagaa tgcatgatgg agtttcagtt agaccaagag tggcggttac
    15181 gtcccatcaa aggcgacaca ggaaaaacat acataggcat gcgtgatgag gacaaagtct
    15241 ttattaaacg aaacacgact ccgatgttgg ctgctctttc aagggaagga attacaccaa
    15301 aacttgtttg gacaaaacga acggggaatg gcgatacttt gacagcacaa gagtggctgg
    15361 aaggtcgctt gctgactcct aatgagattg gtcaaagaaa cgacgtgatt gatgttttgt
    15421 accatttaca tcattcaaat tctcttaaaa ccatgctgac ccgtatcggt ggtcgtgtca
    15481 tgacgccgga gaagatgctg caaaaatatg aaaaagaatt accggaagaa ctccagaaaa
    15541 atcaatttct gcaaattgtc catcgttttc ttcgtgagca gatcccgtca atagataagg
    15601 aaaacttatc agtagtacac ggagatgtaa accatcgtaa ttggttagta tgtaaaaatt
    15661 atttatatct agtcgattgg gattcaatca tgtttgcaga ccctgcggtg gatattgcaa
    15721 cgattttagg aaattatgtg ccactgtcta gctggaatca gtggctgatc agttatggca
    15781 tacgcccgac tgacgaaatg cttgaaaaat ttcattggta tgcggccatg aatttgctcc
    15841 aagaaatcac cagatattgc aaacgtgaag atcatcgacg gatgaacgaa gaaattttac
    15901 aattaaaacg aattttcagt ggctgaggtt tggcccaaac tctgcggaga tttggccaga
    15961 cctttttaat tagaggaaag gaatggagat aaataatgcg tgtccgaaat cgtccgggag
    16021 cagcggaaat gttagcggct cacccgaatt ttgtaattag tgatccaaca ttgtggaagg
    16081 gaaaatggaa tgaattgttt gaaaacgatc atccaatcca cattgagatt ggtatgggga
    16141 aaggccagtt catcactgga atggcaaaag cccatcctga aatcaattat attggagtag
    16201 aaatgcaggt cagtgttgtt tcgattgcac tagacaagtt gatcgaacaa ccgcttccta
    16261 acctgaaact tctacatgtg gatggatcag cgttgacaga gtatttcgct gacagcgaag
    16321 tagatcaaat ctatctgaat ttttccgatc catggccaaa aaaacgtcat gaaaaacggc
    16381 gattgacata taagactttt cttgctgtag atgaacagat tcttcgtcca aatggagaaa
    16441 tccatttcaa aacagataat caaggattgt ttgagtattc tctggcaagt ttttctcaat
    16501 atggcatgat cctcaaacag gtatggttag atttacatca aagtcaattt gaaggcaata
    16561 ttatgacgga gtatgaagaa aaattttctt caaaaggtca acgtatttat cgagtagaag
    16621 ctcaatttca agataagacc aaaaagaaaa gtctgtgaaa ctaactttct gctttcataa
    16681 aaacattaat atccataaat aaatctcaga aaatgaacat tacagatgta atgtattttc
    16741 tgggatttat ttgatttaaa atgtatttat ttcatcggtt tttccacaag gtggaatcaa
    16801 ttggaagcga gaaaatgaaa ataatccttt gtcggtgatc ttcaattcgg gtatgacaga
    16861 taatgttaag aaagaaagtg tcaaaaaagg atcaaaggtt ttagctgctc ctagttgatg
    16921 tgctttagtt attaattcat tcaactgttc atagacgatc gaaaaaggtt cctgtgtcat
    16981 gattccgcct ataggaagag aaacacaagc tatttcctgc tgtttgttga caacggtcat
    17041 cccgccacct ttttgaatca aaagattagc agcatacagc atatcctcgt cgtttgtccc
    17101 aacgacaact agattatgcg agtcatgggc aatcgttgtc gctatcgctc cttgtttcag
    17161 ctgaaatcct ttcactatgc ctaatccaat gtttcccgtt tgatggtgac gctcgatcac
    17221 cgcaatcttt aattggtctt tttcgattga aggaatgaat cgttcgcctt gtcggtccac
    17281 tttttctacc aaatctttag tcactagact attcgggata atttcgatca catgggcttg
    17341 tgaagaagaa agaagcagct ctaacgaatg tggatttaat ggttgagcgt ttagttttgg
    17401 cagacaacac gtatatgaat ttgaatgatc agcaaataaa tggtcttgga gttgcccaga
    17461 ttgaacaact aatgttccct tatagaaaac ttcatgaatt ggcagcttgg taagatcgtc
    17521 tgttaagaag aaatcagctt gatacccagg ggcaatcgca cctagtgttt tcaaatcaaa
    17581 acattctgct gcattcaatg ttgccatctg aatggcttgt aatggatcga ttccaagtgc
    17641 aatagcttga cggacgatat gatcgattga tccttctttt actaaatcat tgatcagttt
    17701 atcatcggtg acaaagaagc atcttcttga attttcaggt gtgatcgccg gtagtagcga
    17761 tgccagatct tttgcaacgg tcccttctcg aaccattaaa tacataccaa gatccaaacg
    17821 ttctttagct tcttttgcag ttgtagcttc atgatccgtc cgaatacctg ctgcaggata
    17881 aacatttaac tcttccatgc cgataccagc tgcatgcccg tcaatttttt tcttatgctg
    17941 gtgcatcaat tggatctttt ctaggatgtc tgtttcatta tttgctactg cttgatagtt
    18001 catgacttca gctaagccta aaacttcggg tttttgtaaa aaaggggcta atttttttgc
    18061 atttaaattt gcaccattgg tctcaaaagg ggttacaggt acacaagaag gtagcataaa
    18121 aaagatattc atgagtgttt gtcttgcatc ttctatcata aactcaagac ctgtactccc
    18181 agcaatgttt cctatttcat ggggatctgt cactacagta gtaactccat gaagcaagga
    18241 tacttttgaa aattcgcttg gtgataaaag tgagctttcg atatgcacat ggccatcgat
    18301 gaatcctggc actagatatt gcccattagc attatgtacg ctttttcctt cgtattcacc
    18361 gattcctacg atcattcctt cgcagattgc tacatctttt tccatgattt ctttcgtaaa
    18421 gacgttgacg atcttagcat gcttgatcac taggtcagca ggcttttttt tacttgctac
    18481 ttcgatatat ttccttaatg ttgtttccaa tggagtgcct cccaagataa atttttataa
    18541 gtataacata aattcggctt ttgccaatat ttttttgtaa attcatttta tttgaaacaa
    18601 aaagatcaaa aagacgtatg ttttttgtgg aggggagaat aatggaaata aaacgattat
    18661 cggatcaaat gataagtgaa agttttgcca aacatgccca agacgtatat atattgccag
    18721 cttcagaagt attttacatc tattttcgct atggaaaaaa acgaacattc tggaagaaag
    18781 tctcttcggc agatggacag caactgatct caagatttaa gtatttggga cagatggata
    18841 tcggagaaaa aagaaaagct cagttaggtg catttactta ttctttagaa aaacaggaag
    18901 ttcgtctgcg gttgtcttct gtaggagatt accttcaacg agagagttta gtgattcggc
    18961 tattatacgg tacttttgaa acaccccatc gttgctttag ttctcgagat tttgcaacta
    19021 ttttacagaa aaccgtcaga cgtggattat accttttcag cgggacagtc ggatcaggca
    19081 agacttctct aatgtatcgg ttggctttgg aagaaaaatt gcaagtgatt actatcgaag
    19141 atcctgtaga gatagaagaa ccgttttttc tgcagctaca gatcaatccg aaaatagcac
    19201 agacttatga tacacttttg aaactcgcat tacggcatcg tccagacttg ctaatcattg
    19261 gcgagataag agatgaatca actgcacatg ctgcggtaag agcagcacta acagggcacc
    19321 gggtttttgc aaccgtacat gcacgcaatc ttgaaggtac attggtcaga atccatgaac
    19381 tgacaaagaa aaaagaagag ctatctgaat gtttagctgg tgtcatttat caagaacttt
    19441 tggaagatgc caatcaccat ccttctgttt tatggagtta tcaatttatg gaggaagaat
    19501 cagtaaaaaa atcatgggag gaaagttatg aagaagctca ggaaaatatt tggcagtcag
    19561 aactattcta aaggattaac aagaagccag caaaaccagt ttgcacaaac catgggagaa
    19621 ctgcttttat ctggttttag tctgcaagaa tccctttatt tattactcac gatccgttcg
    19681 tttccacctg ttattttggc aaatatccaa catcagctaa aggaagggca agcgtttagt
    19741 gaggtacttt cgcaaatagg atatggagca gagcaactgg tacagatcga gttagctgaa
    19801 cgtcatggga atctgccgac aactttgttg gaaattgcca aacagtttcg gcttgttgaa
    19861 ggatttcgca atgatttgaa aaagctgctt gcatatccga tgctattgct cacctttttg
    19921 ctaggtatct tgtttagtat gcggttgttc gttttgcctc agcttattgg ttcaggaatg
    19981 gtcatgcaag agcattgggg tattcagctg atacaagtct ttcattggta tgttctacta
    20041 ttcatcctag cggttactct attggcttta ctagtaaggt atcgtctgat gaagttgtct
    20101 cctgttgatc agagtgaatg gataagcaga caattattga ttggaccgat atattccttg
    20161 tatcaatcgg cttatttgtc actagagatt ggaaagcttt ttcatgaagg tttcgaatta
    20221 aggcaaatca tagtctgctt aaaggaaacg aaacaagaaa gtctgattca gcatcttgcc
    20281 tctaagttga ttcaaggatt ggaggcaggt atgtcattgg ctgagcagtt ccagaattat
    20341 cgttttctat cagaagaatt cagtaaaatt gttttacagg gagaggcaaa gggaagttta
    20401 ggtaaagaac tgatttttta cagcgaatgg acaagaaaac agcttttcaa acgtcttcat
    20461 cactggatgc attttattca gccaatcatt tttttgggcg tagcggcttt gattttatta
    20521 gtttatgccg ctgttttatt acctgtttac ggaagcatag aggaggtttt gccatgaaaa
    20581 agagaaaaat atcttattat agcggtttta cattgatcga aatgctaatc gtcttactga
    20641 tcatcagtgt attagtgttg ttgttcgtcc ctaatttgtc gcgttatagg aatcatgtag
    20701 accaagaaag tcgtgaagcg atcatccagt tggtagacac acaaaaagag ctctatgcgc
    20761 ttcaaaataa tggaagagtt cctactgtgg aggaattgtt gaatgaaggc tatatcaaac
    20821 gagaacatgc agaaatctat cagcggcctt gaaggtaagg ttttagcatc aggatatacg
    20881 ttgtttgaat cactcttact gattttatta cttacaacga tcctttcttt tccagttctc
    20941 gcattttctg catggaagaa tgaattggca gtctatcaat ttttcggcca atttgaaaaa
    21001 cgaatctatt ctacgcaaaa aatcgctatt gtgaatcaga tccaaacagg cttttattgg
    21061 aatagtgaag agaatcaaat catttttcat gttccgaatc cagataaaat aaattgggaa
    21121 gtattagata ttcctgatga gattaccttg aaacgccatg cttctattac ttttgctgct
    21181 agaaccggga atgaaagttc actaaaagct tatcaatttt attgggaaga aaaagagcgg
    21241 acgattacgt atcaattcca gatagggagt ggtcgctata caaaaagaat tgaataaaac
    21301 aggatttgtt ttgttggagt cccttacagc gcttatgatc gcttctgtct gtgtctactt
    21361 agtgagcttt ggccagctgt ttttgcttca ggcagaaaag aaaagtgggg aggaactgct
    21421 atttattcga atgatttatg aagatatcaa gacgcagcgg atgtatcaca aaagaccaga
    21481 gcgaagggaa gaaatgatcc catacgatat ccaaattgtc gataataatg gagtaatcga
    21541 aaaagtgttt gtcaatatgg agaaagaagc gatcagtatt gaaaaaacaa gtaattccag
    21601 catttacgct gattgaatgt ctaattggac tgatgatatt gagcagtcta ttgctcacat
    21661 tcaatctttt gattcagcaa accatccaag taagtgaatt tttgcagcgt aaagatcaaa
    21721 aagagtggct aatattttta gcacaattag aagaagaact caagagcagt cacaatgtct
    21781 ctgtgaaaaa tcagcaactg cactacacga tcgaggataa acagtatatc atcgaaagat
    21841 atcaggcaat gatacgtaaa agaagaactt ccggcggaca tcaacctatg ttaacaagta
    21901 tcactgagct tcaattattg gaaaaagaac acacaatttc tttttatgta cactttgaaa
    21961 atggagaaga tggatatgcc atatggacaa aaaacgatca gtagaaaatg ttataagggc
    22021 tctattttat tttctgtctt gcttttattt cttttatgta cgtttttatt gatggcagca
    22081 ttagaaagct accgtttgtc aatggatctt aatatacgaa tcaaaaatta ttatatagca
    22141 aaaataatga agcagatggc tttatcggaa cagtctacgc ttgccgaaag ttcggaagga
    22201 gttttttact acacaactgg atctgtaaca tatcagtggg tacaacagtc cctattctta
    22261 gaggtggaag tctcaccgtt tatctttcgg ttcacagaag aagtaaaaaa tgatacagat
    22321 actagtacag aatgatggaa tgttcgtgaa aacgccctct tttctgttat aacactgttt
    22381 ttaataaaaa aatgtttgcc gtttcacaaa taaaaaacag tgaaaacgat tgaaattttg
    22441 tcaattgttc gatagaatta aacaggtgaa aaaattgtta taatatatct agaaatgagg
    22501 tgcaccatgt taccagaaaa aatggaacaa gggtttaatc aaaacctgga agctattcag
    22561 cttttacaga atgcattggg aacttccttt ttagaagcct atgtcgaaaa tgcagaaaat
    22621 ttaatagatg attatcaagt acgtgtggtg gatggagtgc cgacaaaaga gacaacgcaa
    22681 cggatcacag ctctttatga agagttgaag aaactttctt tcgagccaga agaatggcgc
    22741 agactgtctc aattactttt attgaagggc agccagacag aacatttgca ggcgaaccat
    22801 caactcactc ctgatagtat cggtttttta tttgtttttc ttattgaaca actatacaca
    22861 aataaaaaag caccggttaa gattctggat attgcggcag gaatgggcaa cttgttatta
    22921 acagttcttt taaacctatc gaatgcgggg taccaaacag aaggaattgg tgtggatatt
    22981 gatgatacat tgttggctgt cgcagcttct acaagtgatt tgacacaagc aaatgttcaa
    23041 tactttcatc aagacgggtt gcaggaatta ttaatagatc ctgtcgattt tgcaatcagc
    23101 gatttgccaa tcggttatta tccgaatgac gaaaaagcaa aagagttttt aacaagtacc
    23161 gaagaaggcc atagttatgc acatcattta ttgctagagc aatcaatgaa atatgtgaag
    23221 ccggatggtt ttggtttgtt tttgatgccg agtggatttt tagaaacgga tcaaagtgaa
    23281 gaaatcaaaa aatggtttaa agaagaaggt tatcttcaag gaatgattca attgccagat
    23341 gaattgttcc gtaataaaca atcgcaaaaa agtattttga tcttacagaa aaaaggacca
    23401 caagcacaac aagtaaaaga agtcttgctt gtgaaacttg cttcattaaa agaaccagag
    23461 aaggtgacgg aatttttcaa cgagtttaaa aactggaaat catctgctct gtaatcattc
    23521 aacgagaagg agagtcatat gtctaaaaca attgcaatca atgctgggag ttcaagtttg
    23581 aaatggcaat tatatcaaat gccagaagaa accgtagttg cgaaagggat cgtcgaacgt
    23641 atcggtttac aagattctat ttttaccatc aagtacggcg ataatcaaaa attcgaacaa
    23701 atcatggata tcgaaaacca cgaagtagca gtaaaaatgc tgttagatca gttgattgaa
    23761 ctgcaaattt taagctctta tgatgaaatc accggagtag ggcaccgtgt tgttcacgga
    23821 ggagaaactt atggggattc agtagtgatc gatgagaaag taatggaccg cattgctgaa
    23881 ttagcagaat ttgctccttt gcataatcct gcaaacttaa tggggatcaa agcgtttaaa
    23941 aagattttac ctgacatttt gagtgtggct gtattcgata catctttcca ctcaacaatg
    24001 ccaaagcaaa attatttata tagcttaccg gtgtcttatt atgaagattt caaagtaaga
    24061 aaatatggct tccatggcac aagtcatcgt tatgtgtcgg aacgtgcggc aaaattattg
    24121 aacaaaccga tcgaagaact aaaaatcatc acttgccact taggaaatgg tgtatcgatc
    24181 acagcagtcg atggaggtaa atccatggat acttctatgg gattcacacc attagctggg
    24241 gtgacaatgg gtacacgttc aggtgatatc gatccagctg ttctcccata tttaatggaa
    24301 aaattagaga ttgatattga tgaaatgatc aatgttttga acaagaaatc tggtctgcta
    24361 ggcctaacag gattgtctag tgatatgcgt gatcttgaaa agaactatga aaaagaacat
    24421 attcgtttag cttatgacat tttcgtggac cgcatccgaa aatacatcgg cggttatgtg
    24481 acaacgatga atggtgttga tgcaattgtc tttactgctg gtatcggcga gaatgacgga
    24541 catgtacgta gtgaaatcat caaaggtatg tcttggtttg gttgtgaaat cgatccagcg
    24601 ttgaatgaat tgcgtggtga agaagtagat atctctacaa aagaatcaaa agtaagagta
    24661 ttagtgatcc caacagatga ggaactaatg attgcccgtg atgtggagcg tctgcgcggc
    24721 taagccaaag cattcgttag gataaaagat gaaacgcatg caagagggaa ttcttgcatg
    24781 tgtttttttt gcggagaaga ccgcaaaatg gtaatttaac ttcaatttcc cttctttttt
    24841 acaggatctt cttagggaat tttgcttgga aaagtggtca aaagggaaaa gactggcatt
    24901 ttatttattg attttcagga aaatcccaat attttatttt cattcatctt tataagggac
    24961 attacatatg taaaaagaaa aaacattttt agatcagcct cgtgggctgt cgactttatt
    25021 cttcgcttta tgtttagcta gaaaaccgat ccgaaacatt cattgatcga ataataaaaa
    25081 cttgattttc cacctgtact ttgcctgaat cgtgaaagca caggtatgaa aatcaagttt
    25141 tttgttgcga aaaaacgtcc tagtcaatca gttggccgag cggctggcgg ctgatctgct
    25201 cctgatcaat aacgagtgct tcttggaagt gttgctgcaa aaagtagcgg tcatggctga
    25261 ctgctaggag cgttccttga tagtcttgca gcatttcttc aatttcttct cgagcatagc
    25321 tgtcgaggtg attggttggt tcatccaaaa tcaggacgtt gattttttct aaaaagagtt
    25381 tcagcaacgc caaacggatt ttttcaccac cggataaatc tttgatccgt ttacccacat
    25441 cgctttgata aaagccactt ttggctaatt gccgtcgagc gacttcttcg tttccagtca
    25501 atgtgatggc gtaagccagc aaacggctat cgggatcggc aaactgcagc tgctgcggca
    25561 aataaccgat ttgcaagcta ctacctaatt tgatggtgcc accgtaaggc ttttcctcac
    25621 cgagtaataa tttgagtaag gttgattttc ctgcaccatt gtcgccgatg atggctaaat
    25681 gatctccacg atagactgca aaactgctgt ctttgaatag tacttgatca gcgtactgct
    25741 ggtgcaagtt ttgaatgatg aaaacttctt tgccagattt tccaccatta gacaagcttt
    25801 gcagccgatt tttcggggga ggtggcggtt tgacgagtgt cagtttcgcc aggcggcgtt
    25861 ccagttcttt ggcttttttg aagaaggact cattgtctcc ttcatgtgcc cactgacgaa
    25921 aacgacgaat catgactttc aaccgctgga cttctttttg ctgcagttcg taattttttg
    25981 tcagctcagc caagcgagct tctttcaaga ctcgaaaacg gctgtagttt ccgggatact
    26041 caatcaatgc accttcttcc agttcccatg tttttgtggt gacttgatcg agaaattgcc
    26101 gatcatgaga aacgaccaga accgattttt ttgtattctg taaatagttt tccaaccagc
    26161 gaatactgtt aagatctaaa tgattggtcg gttcatcgag taaaagtaaa tcggcatcgg
    26221 ctgctaaaat tttggctaaa gcgactttaa ctcgttcgcc tccgctaagt tctgttaacc
    26281 gtgcttcctg acagtgtgga atccctaagc cttgcaagat cgctgcaatc cggtcttcta
    26341 atacgtaacc gcctagttct tcaaagcgat gctgcagctc accgtaacgg gccagacact
    26401 tctctaagtt tgtatctggt tctgtcatca atgcttcgta gcgactcatt tgctgtttga
    26461 ggtcctgtat ctcagcggca gaatggagca agtagtggtt gacggtcgct tcggaaactg
    26521 taagttcttg ttcgaccgtc ccgatcttta agcctttttt gcgactgata acaccgctgt
    26581 cgacaccttc tctgccggtc aagatttgta aaaaggttga tttgccagtt ccgttgatat
    26641 cgactaaggc aattttttca ccagcttcaa tttgacagga taaatcctca aataatggat
    26701 tcccgccgat tgttttttta atagaaattg cttgtatgat catgatctct ctccttgaga
    26761 ccactcaaaa aggccatggt tatccctggc ctgtgttatt taaactcaga ttatcatgag
    26821 aaaaatacct tgagatggcc attgatttta cgtgaattca actgttttgg acaaacttgt
    26881 cccgtcaaca gcaactgtaa aaccaattgc atggctaaat gcgatatggt atcgttggta
    26941 attttcatta cttgttattt tagtcatgcg catcatagta ttttccctcc ttcgtgtagt
    27001 tgtcttttcc agtgtacctg aaagtgtcag aaagttcaac tagcgctgac atttacttaa
    27061 tatttatcga tgtgtaattc tgtcagagag ctttttttat ctgactgagg gtaatgtctc
    27121 gtggaccaca tcattgtatt ctgagctaaa ccagcgttca ggcgaaatca tatggtgttt
    27181 gataaaaaac tttgtctaca tgacttcctt ttgccatttg cttaataccg ttgcattaaa
    27241 cagctcttct ctttggaaat ctcaccaaca tcgcgttcag gagtatcgtt gtggttgcaa
    27301 tggcaagcgg tagcctttca tcaattcttg aatgatttac cgaatgtagc acttgccttc
    27361 tcgcggtacc cgaacccgaa ataaaatttt agtttggcga taaaatcggt atccgccatc
    27421 gatacttcat attttttcgt atcagattca tcggaaactt ccacattgac gaaccaataa
    27481 gctttggcat gcttaggtcg ttttaaacca caccagggcg actaatcgcc ctggtgttca
    27541 ctttttttat tttacttgaa ctcattgact gttttccctt tattttaaac attataaaga
    27601 ccgatgagtg cttgccgcgt cttttgtttt tatcacggtt ttttgtgcaa aaaaacctgc
    27661 cgatagttgg caggtcgtct acgtgttaag ctttggtgtg gtctttagct ggcaattggt
    27721 tttctaaatc agttcggaca acaagcacat cgcaagctgc gtttctaata acatattcag
    27781 aaacagaacc aataaataaa cgttctacag cattcaatcc tgtggctcct aacataatca
    27841 aatctacttg attatcttct gggatttctc tggcaataat ttgttttggt gagccatatt
    27901 ctacgacaga ggttacatta ttcaagccag ctttcttcgc attgctttca taatctgcca
    27961 atgtttgctt tgccatttca gtagcttgtt ctgcaagcat tccatcaaag gatgaaacgg
    28021 tttggaaaga acgtgtgtca atgacatgtg ctaatagaag ttctccgtta tttcggatag
    28081 ctacattgac cgccttttta aaagctaatt cagcttcatc agaaccatct acggcaacca
    28141 tgattttttt gtattgttgt aacataagca ttcactcctc aagtcaatcg tattgtactc
    28201 ttttattgta gaacacttta gggaaaaaga gaattattta ctgctttggt tcagcaattt
    28261 tttttgcaaa tcggatactg aaggtggcag aaacaataat tacgatcagt aaaaagacca
    28321 aggcaataaa gcgttgattg aaaaaagctg ttaaaatacc cattacgcct aaaacagcat
    28381 atgcagcacc gtaaaattgt aggaatcctt gatttttatc atttttgttg atcaatacaa
    28441 aaaagatatc ctgtttatta aataaataat agctaacgaa taataatact aaagtggcga
    28501 taatcattaa aatcctaatc atgaacaaat cctccaaaat aagcaacaaa tagaaaaagc
    28561 aatttgccaa gagcaaattg ctttattctt taaaagatgg taatctccgt taatgccgtt
    28621 gtttgtcctg tagtattgat cccgcgaatt caagcaggtg ggtcccatgg tcgtagcttc
    28681 gaactaccaa atgataaggc atgttaccag cagtgacaca agacgggtcc caagatatca
    28741 ataaaaatgt tcggtcaaca cacaaagagg acaattcgaa catcacagat ttcccacctc
    28801 tatagtagca tatgaattta aaaagagcaa tgagagagct cgcagaaaca gaaccgtttt
    28861 cttgtctagt tttttggagg atgctttttc cactctttta tacggtaata ctgttgaccc
    28921 agagcctgtt catatttccc tgtatctttt ggctgataat actgagcatt cttcagttta
    28981 tctggtaaat attgctgatt gacccaagcc tgatcaaaat gatgaggata ctgataaccc
    29041 acgccacggt tgagtgattt cgcacctttg taatggctat ctctcaaatg atcaggaaca
    29101 tctccagctt tgccctctcg aatatcagca agtgctgcat ctaatgccat gtaggcagaa
    29161 ttggattttg gcgacaagca aagatcgacg acgacgtctg ctagtgggat acgtgcctca
    29221 ggcaatccca atttttcagc agctaataca gcgttcactg tacgagccgc agcagcagga
    29281 ttacctaaac ctatatcttc ataaccgata accatcagac gccggcaaat acttgccaga
    29341 tcacctgcct caactaaacg ggcgagataa tgaagtgcag catccacatc acttccacga
    29401 atcgattttt gaaaagccga aataacgtca taatgagcat ctccattttt gtcatgggta
    29461 agagcttttc tttggacaca ttcttcaatg atcgacagtg taagatgaat ccttccttct
    29521 ttatctgaag gagtcgatag tgtagccagt tccagcccat tcaatgcact ccgcaaatcg
    29581 ccgttcgttg cacgcgaaag gtgaagaaga gcatcttcgt ctaactggat tgcttgttgt
    29641 ccaaggccgc gttctttatc ccttaaagca tgttcgactg ccaactggat gtcttgttct
    29701 gttaatggtt tgacttcaaa gatctgtgta cggctgcgga tcgctggatt gatggtaata
    29761 taaggattct cagtagtcgc tccgatcaag atgattcggc cactttctaa atgaggaagc
    29821 agaaaatcct gttttgtttt atctaatcgg tgtacttcat ccaataagag gatcactgta
    29881 ccactcattt ttgcttcttc cgctacaact tgtagatcct tttttgaatc tgtagcggcg
    29941 tttagcatgc gaaatgcata attagtagag ccggcgatcg cacttgcgat actcgttttt
    30001 ccagtaccag gaggtccata tagaatcatg gaagagagca tgcgagcatc gaccatgcgg
    30061 cgtatgattt ttccttcccc gactagatgc tgctgaccaa cgacttcatc gatcgttcga
    30121 ggtctcatgc gataagccaa tggctgttgc ataggatttt cctccttctt gtttttacgt
    30181 cccttaatta tatcatattt agcaagatgc ctagaaagaa cagccttctg ttgttttgtt
    30241 ttcatagctt atccaactaa tattgtgtat gataaagtag gtgagaaaaa tgacggataa
    30301 acaaaagata gatgaatttt tttggaataa aacgacgcca gaagtggcac gggatttatt
    30361 ggggatgtat ttggagcatg acacaccaga aggaagatta gctggatata ttgtagacgc
    30421 ggaagcatac ctaggtccag aagacgaggc cgcccatagc tatggtttaa gaagaacacc
    30481 gcgtgtgcgg gcaatgtatg aaaaaccagg tacgatctac ctttacacga tgcatactca
    30541 tcggattttg aacatcatta ctcagccaga agggattcct caaggagtaa tgatacgtgc
    30601 aatcgaaccc gccgcaatga ttgatcaaat gagtaaaaat cgtggcggaa aaactggtcc
    30661 agatatcagc aacggtccgg gaaaattagt ggaagcatta gcaattcctc aagaacttta
    30721 tggtcagtct atagcagata gttcgttacg cttagttttt gagaaaaaaa agacaccaaa
    30781 aaagatcaat gctttgccta gaatcggtat cccaaacaaa ggtgtatgga cagaaaagcc
    30841 gttgcgtttt gttgtgtcgg gaaatcctta tatctcttta caaagaaaaa accaaatcga
    30901 gaaagattgg ggttggagga aagaaaatga aaaagaagga tctattaaca tatttagatg
    30961 aaataattga aaaacaagtg acagactatg atgtagcttt agattgggat acaaaaaatc
    31021 atacgatcga gatcgttgtt cgcttgtttg cagaaaataa agcacaattt gagattgatg
    31081 atgcagaagg gatcgtttcg caagaaccaa ttatcgaatt tgaagacggc gtacttcttt
    31141 ttaatcctca gaaatctgtt tttgatgaac gcaattatct agcagtaatc ccttatgaag
    31201 gaaaaaaagg gttggcaaaa tcagtagctg attctttagt ggaatatcta aatgaagtat
    31261 tagctcaagg gcaaagcgat ttgcttgatt ttttggatga agacgatgac gaagctgtat
    31321 tcgagcttca ctgggataat caacaactag cagaattagt agaagtaaaa cagcaaaatg
    31381 aagcagatac ttatttaccg tacccaagtt attaaggagt gacagatatg aaatggaatg
    31441 aagtaaaagt agaaactgaa agtgaagcag tcgaagcagt tgctaatatt ttgatggagg
    31501 caggggcgag tggtgtcgcg attgaagatt ctttagatgt tgaacatttt cagtcggatc
    31561 aatatggtga gattttagat aaaaatgatt ttacatcgct gaaagaaggc gcactagtca
    31621 tggcttattt tccggaaacg actttcttac cggaaatctt gccttttatc aaagatcgta
    31681 tcacacaatt acctgagttt ggtctagcta ttggaaaaaa taaagtaaca gttagtgaag
    31741 tagaggaaag caactgggca acagcctgga agaaatacta tcatccggtg cgtattacgc
    31801 gaatgctgac ggttgcgcca agctgggaaa actaccagac agaagatcct ctagaaaaaa
    31861 ttatcacact agatcctggt atggcatttg gcacaggcac tcatcccacg acttccttga
    31921 cattgacagc actagaaaca gtcttgcgtg gtggtgaaac attgctagat gtgggaactg
    31981 ggtcgggcgt gctaagtatt gcagcaaaat atcttggtgc aaaagatgtc tatgcctttg
    32041 atttagacga agtagccgtt cgttcggcga aagaaaatat ggatatgaat gaaatagcca
    32101 aagatgtcca tgtatcagct aacgatcttt tgacaggaat cgagattgaa agtgatgtaa
    32161 tcgttgcgaa cattttagca gatatcatct tattgatgat cccagatgct tggcgtttac
    32221 tgaagcaaac aggcacgctg atcgtttcag gaatcattga agagaagaag caaatggttc
    32281 tggatgccat gttagaacaa gggtttatcg tggatcaaat tttccagcaa aaagactggt
    32341 atgcaattat tttgaaaaaa ccagaggtgg aatagatgca gcggtatttt ttaaaagaat
    32401 catatgaatc aaaagactat tttgttttag aaggggaaaa ctatcaccac attgtacggg
    32461 ttatgaggat ggaacccgag gatgaagttt ttttggcatt caatgatcaa atagccattg
    32521 ttgcagaaat cacggagatt actgaagagg ctgtttatct gaaagaaatt aaaaaagaag
    32581 aaaatcaaaa ggaactgcca agttcgatta ccatagcgag tggctatcct aaaggggaaa
    32641 agctggattg gatcgttcag aaatccactg aattaggagc gcaagctttt cttggtttcc
    32701 cggcatcagc ctctgtcgtt aaatgggatg caaaaaaacg tatgaaacga caacaacggc
    32761 ttgaaaagat tgctcaagag gcagcggaac aatcccatcg gcagatcgtg ccatcagtag
    32821 agttattgga aacgaaacaa gatttatttg agcggctaga tgattatgac gtggtcttgg
    32881 ttgcttatga ggaatcagca aagcaaggcg aaaaatcaaa tgcagccaaa attcttagtg
    32941 aaacaaaacc agatagtcga atcttagctg tctttggacc agaaggtgga ttatcccgcg
    33001 atgaagtcga ttattttgta gaacacaaag ggatactttg tggattagga ccaagaatcc
    33061 ttcgtacaga aacagcacct ctttatttat taagtgctgc tagctatcaa tgggaattaa
    33121 tgaatccttc tttcagctaa cgttcttcat taataaaaga gtatcgacag aaagaattga
    33181 aaaaatagtc aacaatttgt ataatagaaa caattattta tacaatggat acgaaaaatc
    33241 gtttgaaaaa gcttttaaaa agcgaacatt tccgtaaaaa aacgtataac tgcacggatt
    33301 ttgggacaat tatcaaacag atatttgtcc cgattcccgt tttaccgaaa aaagaaaggg
    33361 ggatcgattc ctgtgacaaa agaagaaatt ttaacgggac ccggtgtcat aaagctcgtt
    33421 agtcaataca tgggtcccga acatgtagca tttgttgaga aagcatgcga atacgcgact
    33481 gctgcacatg acggacagtt tagaaaatca ggcgaaccct acattattca tcctatccaa
    33541 gtcgcaggta tattagcaga tttaaaaatg gatccccata cagtggctac aggcttctta
    33601 catgatgttg ttgaagatac agaaatcact ttagaagatc tgagagaaga atttggcgat
    33661 gacgttgcta tgttagtgga cggcgtaacc aaattaggga aaataaaata taaatcccac
    33721 gaagagcagc tagcagaaaa ccaccgaaag atgctgctag caatggctca agatttacga
    33781 gttatcatgg tcaaattagc tgatagacta cataacatgc gtacgttgaa gcacctgcga
    33841 gaagataagc aaagaaggat cgctcaggaa actttagaaa tctatgcgcc tcttgcacat
    33901 cgtctaggga tcagccggat caaatgggaa ttagaagata cagcacttcg ttatctaaat
    33961 ccaaaacagt attaccgtat cgtccacttg atgcagacga agagagaaga acgtgaaaaa
    34021 tacgtaagtg gtactgttga agatattcga atagcgacag aagagctggg gatttttgca
    34081 gaaatctatg gacggccaaa acacatttat tcaatctatc gtaaaatgaa agatcagaaa
    34141 aagcagttca acgaaattta tgacttgcta gctattcgag tgatcgtaga ttcaatcaag
    34201 gattgttatg ctgtattagg agcaatccac acaaaatgga aaccaatgcc cggcagattt
    34261 aaggattata ttgccatgcc aaaagctaat atgtaccaat ctttgcatac taccgtgatc
    34321 ggtccggcag gaaatccggt agaaattcaa atccgaacac aggaaatgca tgaaatcgct
    34381 gaattcgggg ttgctgcaca ctgggcctat aaagaaggaa aaaatgaaaa agtagaacca
    34441 gatggtatga cgaaacaatt aagctggttc catgagatac tcgaacttca agacgaaagc
    34501 tatgatgctt ctgaatttat ggaaggcgta aaaggagata tctttagtga taaagtctac
    34561 gtcttcacac caaaaggaga cgttactgag ttaccaaaag gatccggacc attggacttt
    34621 gcatacagta tccatacaga tatcggtaac aaaaccactg gtgcaaaagt aaatggcaaa
    34681 atggtgcagc ttgattacaa attgaaaaac ggagatatca ttgagatcat gacttctcca
    34741 aattcatttg gcccaagtcg cgactggttg aaattagttg ctactagcaa agcaagaaat
    34801 aagatcaaac gtttcttcaa agcccaagat cgagaagaaa atgtgatcaa aggccacgaa
    34861 tccgtggtca aatgtattac agatctagga tttacgccta aagatatttt gacgaagaac
    34921 aaactgcaag aagcactcga tcgttttaat tatcaaacag aagatgatct ctatgcagct
    34981 gtagggtatg gagaagttag ccccttgacg atggccaatc gtctgactga aaaagaacgt
    35041 aaagaacaaa aaatcgagca gcaaaagcaa gaagcagaag aaatcatgaa tcagccgaaa
    35101 aaagaacctg acaaaatgaa agtacgtcat gaaggtggcg ttgtcattca aggggtagaa
    35161 aacttactaa ttcgtatcag tcgctgctgt aatccgattc ctggtgatga tatcgttggt
    35221 tatatcacta aaggcagagg gatatccatt catcgtcgag attgtccgaa tgttcagcct
    35281 gacaaaccaa atgtagcaga acgtttgatt gaagtcgaat gggaagatac atcgaataca
    35341 cgaaaagagt atgatgcaga tttggaaatt tacggctata atcgttcagg cttattgaat
    35401 gatgtacttc aaacagtcaa tgcgctaacg aaaaatctca acagcgttga agcacggacg
    35461 aataaagata aaatggcgac gatccatttg acggttggta tccagaattt atcccaccta
    35521 aagagtatcg tggataaaat caaagcagta cctgatgtct acagtgtacg ccggacgaat
    35581 ggataggagg acagatatga gagcagttgt tcaacgggta agtaaagctg ctgtgtcagt
    35641 cgatcagcaa gaaattggaa aaattggcta cggcctactt gttttattag gcattcatga
    35701 aaccgatacg caaaaagatg tggactatct ggtaaaaaaa attgcacaaa tgcgtatttt
    35761 tgaagatgaa caaggaaaaa tgaatattag tgtagaagat gtgggaggac aaattttaag
    35821 tatctctcaa ttcacactgt ttgcggatac gaaaaaagga aatcgaccaa gctttgtgga
    35881 agccgcacga ccagaaacag ctattccttt atacgaatca ttcaatgaag gaatcagaag
    35941 tcgcggcatc acagtcgcta ccggacaatt cggagcagat atgtcggttt ctctcgtcaa
    36001 tgatggacct gtaacgatca ttattgattc taaaaacaaa taaagtaatc tggttttcac
    36061 tagtaaaaac tatgaaacac gtcgcttaag ttagactgat aaactctaac ttaagtggct
    36121 tttttgtctg caaaaagcgc ttcttttgtc agcagtcgga aaaaaagtta agcttaaatt
    36181 atacaagtta ttttttatag gaggttgctg aagatgaaag aagtaccagt agtagatatc
    36241 aaaatcacag atttaaaaaa attgtatcaa aaaggataca acatgttgga ggagttacgc
    36301 catgaagcgg acgctcccgt cgtcaaagca aagattttca ataaagaagc tatcacgatc
    36361 tacggatcat cagcagcaaa agtattttat gatccaagga atttcaaacg aaaaggtgct
    36421 atgccaaaat tagtattgaa gacacttttt ggtcaaggtg gagtacagac tttggatgga
    36481 gctgctcatc accatcggaa aaatattttt atggatctaa tgacaccgga aagaatggaa
    36541 gattatcatc gtatcttaga taagaatctg acacaagcac ttgaagcaca gcatggtcaa
    36601 tttgaactat ttgatttaag taagatggtt ttctttacat ctatttgcga gtgggcaggt
    36661 atcaatttat cagcgatttc taaagatgag gtcgaaaaat tagcagaata tcaaatctct
    36721 atgatcagcg gaacgtttac ttctccgatt gatcacataa aaggtgtcga gaatcgcaaa
    36781 aaatcagaaa aatgggcgca aggcttgatt gaagaagctc gacaaaatcc tgtggctgga
    36841 aaagaaaatg tggcattata tgcttttgcc aatgccacag atttagatgg tcagctttta
    36901 cctctggaag tggcagctgt tgaattattg aacatcatcc gtccaacggt tgctctaact
    36961 gtgtgggcgg cattgatggg acatgcactt ttcagtagac cagatttgta tcagcaatta
    37021 aaaaatgact tttcgacgtt acaagatccg tttattcaag aaatgagacg ctattatcct
    37081 ttcttcccaa tgctacctgc gatttcgctg aaagaagtag aagtagatgg ttatcgtata
    37141 ccagaaggca gttgggttat tttagattta tatggaacag accatgatga acgaacagtt
    37201 gaagcgccag attcctttat gatcaaacgg tatgtaggga aagcaaaaga tatttcctat
    37261 aaagaagaat atgagatgat tgcacaaggc gggggaaatt ttcgacaaat gcatcgctgt
    37321 gcaggagaat ggatcactct tcatagtcta cgtgtgtttt ccgatcagct agtaaataag
    37381 tttgagttct ctgtacctga acaagattgg acgattccat tcaatcagtt tccgacttat
    37441 ccgaatagcc gagcgctact ttacaagaat tagattagca taaacttatc gaaaaaaagg
    37501 aagatcaaac agtgctgatc ttcctttttt catattacta taaattgata aggtcaattg
    37561 atcaaaatcc acgaaatgct tcatcaatgg tttgataatc ttcattggat aaatgcaaat
    37621 ctaaagcttg gacattgtca gatacttgct ctggacgctt agctccaggt ataactacag
    37681 acactcgtgg attcttcatg taccaagcta aaacaagttg cgcaggagtg gcttggtatc
    37741 ttttagcaat ggggcgaagt acatcgacag cggttaaaat ttctctgaaa cgttctcctt
    37801 ggaaatctgg atttcttttt cgaggatctc cttcaccaaa ttgtttttcc tcaccaagtt
    37861 catattttcc tgtcagcaaa ccggaagcaa gaggaaaata gggaacaaaa ctgattttgt
    37921 ttttttcaag ataagggaat aattcttttt cagcctgccg atggatcaaa ctgtatttat
    37981 cttctactac atcgacatat ccatctgcat tcgcttcttt caattgttct aacgtgaagt
    38041 ttgacacacc gactgctcgg atttttcctg cttcttttaa ttcgtgcaat gtagcaactg
    38101 attcattttt tggtgtgctt tcatccggaa agtggatata aaaaatgtcg atgtaatcag
    38161 tttgcagacg ttttagtgca tcttccaccg attgtttgag aaactcagga gaattatcaa
    38221 atgttcgccc tttatttggt acatgagcag cttttgtagc gatgacgaca cgtgagcgat
    38281 catattcttt ttccttcagt acctcgccaa tcagttcttc ggaacggccg tttccgtaag
    38341 cataagcggt atcgatcaaa ttgatcccgc tattcaaagc tgtacgaact acttgttttc
    38401 ctgtctcatc ttccaaaccg tcaaaaagat tatgtcctcc aacggcattt gctcctaatc
    38461 caagctgttc tgcgtagact tgtgaatgtc cgattcttat ttctgccata gctgattcct
    38521 cctttttgtc tagtctaaaa caaaatacca aaaagctgaa ctaaaacgtt tttgtgaggg
    38581 gaaatagtca gatatctctt gactttgaga ctggttttta gtaggttata agtaatatcg
    38641 aatgtcaatg aaagagcgag taagtttttg aaactttaca agagaggatc atcgaggctg
    38701 aaaatgatct aaagtttcat tagcctaaag acactctgga gatggacgag tgaaaagccg
    38761 tcccgcgtac gagcgttatt cgtaagagga aagagaaatc tttcaaaatt aggtggtacc
    38821 gtgtatgaat ttcattcacc cttgttcatg atgaacaagg gtgatttttt tatttacata
    38881 gctaagaaag ggagagaaaa ctatgagttt ccaaagacca aaaggaacaa atgatatttt
    38941 gccaggcgaa tcagaaaaat ggcagtttgt cgaagaaaca gcacgtttat tattcagaga
    39001 ttatcagtat aatgaaatgc gtacaccgat ttttgaacac tatgaagtga tatcccgaag
    39061 tgtaggggat acaacggata tcgtttcaaa agagatgtat gatttttatg acaaagggga
    39121 acgccatgtg acgcttcgac cagaaggtac agcaccaatc gttcgttctt ttgttgagca
    39181 taaattgttc ggcccagagt atgcgaaacc gttcaaaact ttttatatgg gaccaatgtt
    39241 tcgctacgaa cgccctcaaa aaggacggtt acgtcaattc catcaaatcg gagtagaagc
    39301 atttggatca gaaaaccctg caactgatgt ggagagcatg gtgatggcac ttgatttctt
    39361 caaacagttg gggatcacgc aaatccggct agtcatcaat tcattaggtg ataaagaaac
    39421 gcgtcaagcc tatcgtcaag cattgatcga ctatctttta ccttttgaat cagagctgag
    39481 tgaagactct aaacgccgcc ttcatgaaaa tccattacgc gtattggaca gtaaagacaa
    39541 acgagatcaa aaatttgtgg cagaagctcc atctatctta gattatctaa gcgagcctgc
    39601 tcaaaaacat tttgataccg ttgtttccat gctggatgca ttggctgttc cttatgaaat
    39661 cgacagtaat atggttcgcg gactagatta ttacacacat acgatttttg aaatcatgag
    39721 tgacgcacca aaaatggggg cacaagcaac gatttgcgca ggcggacgtt atgacaatct
    39781 agtggaagaa ctaggtggtc cttcaacacc tggcttcgga tttgcaatgg gtatcgagcg
    39841 attactcttg actatggagg cagaggaagt agttatccct gcatttaatg agttggatgc
    39901 ttacgtggtc gcactcggag acgaaacaaa tatcgaagca ttgaaagtgg tccaagcgat
    39961 tcgaaacttt ggtttttcag ctgaccgcga cttcatgaac cgtaaagcaa aagcgcaatt
    40021 taagacagca gataaagcga atgccaaatt agtattggtg atcggtgaag atgagcttgc
    40081 tagtcaaacg attaatgtca aagcaatggc aagccgaaaa gaaaaaagtt ttccattaga
    40141 agagatatat gaacattttg atgaagtcta tgatgaaatg actttattcg agtaggagga
    40201 aaaaaataaa tggcacaaag aacgatctat tgcggattag tttccgaaca acaattagaa
    40261 caaacagtta cattaaaagg atgggtacaa aaaagacgag accttggggg agtaatcttt
    40321 atcgacctac gtgatcgtga aggaatcgtt caaatcgtct ttaatcctga aatcaacaaa
    40381 gaagcatggg aaatcgcgga taaatgtcgc agtgaatacg tgatcgaagt gactggggaa
    40441 gttagaagac gcggagaaaa tgcagtaaat cctaaaatgg ccactggtga attcgaagtg
    40501 attgcaagtg aaatcgtgat tttgaatcaa gcaaaaaccc cgccatttca aattgaagat
    40561 gatcaagcaa tcagtgacga aatacgtttg aaatatcgtt atttggatct gcgtcgtcca
    40621 aaaatgacta aaaacatcaa attaagaaat gcgacaacaa aagcgattcg tcattattta
    40681 gatgataatg actttctcga tatcgaaaca ccttacctag ggaaatctac accagaaggc
    40741 gcaagagact acctagttcc ttcacgtgtc catgctggac atttctatgc gttgccgcaa
    40801 tctcctcaat tgtttaaaca attgctgatg aatgctggat tcgaccgtta ttatcaaatc
    40861 gttcgttgtt tccgcgatga agatttacgt ggagaccgtc agcctgaatt tacgcaagta
    40921 gatattgaaa caacgttctt aacagcagaa gaaatccaaa cttacacaga gggattgatt
    40981 gcgaaggtaa tgaaagaagt tcgcggtatc gaagtcacat tgccatttcc tcgtatgacc
    41041 tatgatgaag caatggctcg ctacggcagc gacaaaccgg atacacgttt tgatatggaa
    41101 ttgatcgatc taagcgatac agtcaaagaa gtagaattca aagtcttcca aatggctctt
    41161 gaaaacggcg gtgtcgtgaa agcattgaat gctaaaggcg ctgcagatcg ctactcacga
    41221 aaagacatgg atcagttagg tcaatatgta ggacaattcg gagctaaggg gcttgcatgg
    41281 ctgaaagtag aagaagacgg attgaaaggt ccaattgcta aattcatggg agaagcaaca
    41341 gaagcaatca ttaaagcaac agatgcaaaa ccaggtgact tgctgatgtt tggcgcagat
    41401 aaaagtgaaa tcgtcgctgc tgcattaggt gctatccgga cacgtttagg aaaagaatta
    41461 ggactaattg atgaatcgaa attcaatttc ttatgggtaa ctgattggcc acaatttgaa
    41521 ttttcagaag aagaaggtcg atatgtctct gctcaccatc cattcacgat gccaaaagaa
    41581 gaagatatcc cgttattggc tgaagaccct gcaaaagtct atgcagaagc ttatgatatc
    41641 gtcttgaacg gttatgaatt aggcggaggt tctatccgta ttcatactcg tgagcttcaa
    41701 gaaaaagtac tagaaactct aggctttaca aaagaacaaa tggaagaaca atttggtttc
    41761 ttgttaacag ctttagatta cggattccct ccacatggcg gtattgcatt aggattggac
    41821 cgttttgcaa tgttattagc cggagaagat aatattcgtg aagtcattgc ctttcctaaa
    41881 aacggaaaag cagcagatgt gatgagtgat gcaccaagta tggtttcgcc tttacaattg
    41941 ttcgaattga atattgatgt aacgaatata gaagaatagc cgataaaaag gtaaaaagga
    42001 tttgtcgaat acgcttcttt tgacaacctt gatttaattg aaaaacactt tataatgaag
    42061 ccataacata taaagcgggg cttttccatg aaaaaaacat tcgaaaattg ggcttatcat
    42121 gattacacga ccaagctttc ggtatctatc gtttatgcgt tgatcgcatc agtcgcattg
    42181 aatttctttt atcagccagg acatatttat tcaagtggta ttactggtct agctcagatt
    42241 ttgacgactt tgtcaaaatc actgatagga ttcgaagtac cggtatcaac aactttgtat
    42301 ttactgaata tacctttgtt tgtattggcg tggttaaaaa tcgggaagaa gtttacaatt
    42361 ttcactttta tcacagtcac tttgacctcc ctttgtatcc atattttacc ggaaacagtc
    42421 ctatctaccg atccaatcat ctgtgcgttg tttggggggg ctgtgatggg actaggtatt
    42481 gggctagcgt tgagaaacgg cctatcatcg ggcggtctag atatcgtgag tatcacgatt
    42541 cgtaaaaaaa caggaaagtc gattggttct atttcgatct acttcaatgc attgatcgtt
    42601 tttgttgccg gaattctatt tggctggcaa tatatgtttt acagtacgtt gtctattttc
    42661 gtcagtggaa aagtaactga tgtcgtttat actaaacaaa agaagatgca ggtaatgatc
    42721 gtaacgaaac atccagaaaa agtagtaaat gaaatccaaa actgtatgcg tagagggatc
    42781 acgatcatca atgaagcaga aggtgcttat aagcatgata agcagacagt attgatgacg
    42841 atcgttacac gatacgaact gcctctactt agagaagcaa tgaaagcttc tgatccacaa
    42901 gcttttgtaa gtattgcaga aaacgtccag atacttggtc gtttctatga ggatggcatt
    42961 tagatcgagt ttgacaaaaa ttcatgaaat actgctgtag acagacgttc gtttgtctgc
    43021 agtttttttc taccatagta gaatcagcaa agaaaaactg cttttttatt tgaaattttc
    43081 ttaaagttga aaaaaagcat gtttattttt ttaaatttat gggatgatag aaaaaaatag
    43141 tagagaaagg acacgattct acttgctatt ttcacttaat acccgtatat tacgaatgaa
    43201 aaaagtttat gaaaagttgg aatattgaat ggaaaaacaa aaaaggttaa aaaacagccg
    43261 ctacatcact gggtttgatg ggctccgttc attagctgtg gtgggtgtta tcttctatca
    43321 ccttctgcca actagtatga aaggcggcta tttgggcgtt ccgatttttt tcgtcgtatc
    43381 tggttatttg atcacggatc tgctgcggca ggaatgggaa caaaatggaa aaatcaatat
    43441 ttggcaattt tatgtcagaa gaatgaagag actctatccg gcacttgttt ttatgcttgt
    43501 gacagcttcg gcttatatca ctttgtttca gcgagggctg ttaaacaatc ttcgaggagt
    43561 tgtggtcagc agcttactgt atgtcaacaa ctggtggcaa atcaataacg gtttatctta
    43621 tttcgatcga tttgctaatg agtcaccgtt tacgcatatc tggtctttag cagtagaagg
    43681 tcaaaactat ttggtttggc cgatcttgtt tgtattgttg atggtatttg ttcgtaaaag
    43741 aaaatggatc ttttatactg tgttaggtac atccttcctc tccgccatct tgatgatgat
    43801 cttgtataca ccaggaggcg atccaacccg tgtttattac ggtaccgaca ctcgtctgtt
    43861 ttctatatgg ttgggaagtg cgcttgcgtt tgtttggcca agtacgcgtt taaagaaaaa
    43921 catcccgaaa caagcgaaaa atattctgaa tatcgctgga ggaatctcgc ttttggcact
    43981 gattctttgc ttcttctttt tagatgatca ttacaatttc atctattacg gaggaatgct
    44041 cctagtgagt ctgttatgcg tagtgctagt tgcagtgaca gcacaccccg gagcaagttt
    44101 gaatcgatgg ctgacaaatc cagtatttac ttggattggc aaacgaagct atggcattta
    44161 tctgtatcag tttccagtta tgatttttta tgaagcaaag gtgactgata ttgccgatca
    44221 tgttttgttg catactttag tggaaatcac attgattcta atcatcagtg aactttctta
    44281 tcgatatgtg gagcgtccgc tagcacgttt tgattatggt cgtacattcc aagtgatgaa
    44341 aggatggttc agcaaaccga tcatcagtaa gagcaaacca tgggttgttc caggcacatt
    44401 acttgtagca attgcactcg ttggtttcgt tacggcaccg aaaaacgctg taacagccga
    44461 tcaaaaacaa ctgcaggcaa aaatcgctga aagtaaaaaa ttagcagaag aaaccagaaa
    44521 caattcagaa aacaccacta gttcggttga ccaagcgatc cttgataagt acgagttgtc
    44581 caaggcacaa gggaataagg cacaaaaatt agaactaacg gcatttggtg attcagttat
    44641 gttggatgct gcaacggatc tacaagaact ttatccaaaa gtagtggtag atggagatgt
    44701 cggacgacaa ttatatgcca gtgtaccgta cattgaaaaa ttaaaggaac aagatttgtt
    44761 gaaagacact gttctgattg gactaggaac gaatggctca ttctcggaag ctcagtttga
    44821 tagtgtgatg gatgcaatcg gtgatcgaaa agtatactgg gtcaatgtcc gagtaccgac
    44881 gaagcgctgg caaaatgacg tgaatgcgat gcttgaacag atgacgaaaa aatacgacaa
    44941 tatgacgctg attgattggt acagctacag taatgaccat agtgactggt tttatgatga
    45001 tcaagtccat ccaaatcctg aagggatgac acattacatt cacttagtca gcgaagcctt
    45061 gctaggagga agttcaagta cgaacagcag tgagacaaac gtgacagaat gatagctaca
    45121 aaaaacttca ggcaaaaaca tttgtttttg cctgaagttt ttttatattt accgtgtttt
    45181 taacattcat atagattcag gaagcaaaac ggttgagtgt gtctgtaaat aacgttcttt
    45241 ttgttttcgg gtgagttttt tgaatgcagc ttctgctttt gttgcttcgc ttcttgaaga
    45301 aaatacttct gcatggatca tcttaacagg tcttcttttt tgaagacgag tatatttagc
    45361 accagtacca ttgttatgtt cctctaatcg tctagaaaga tcggttgtat atccaccata
    45421 aaatgtctga tctttacaga ataatacgta aaaataatgt tcatttgcca tagagcatct
    45481 cacgaatctc tgcagtgtat tctccatcca tattgtaagt gatcaatggt ggagcgactt
    45541 tgaatccgtc taatttgcct tctttgattc cttcaactaa caaaatattt gcctcttttc
    45601 caggttttgg gtaaacaaat tggatccttt taggtgcaat tcgatagtct tccatgacct
    45661 gaatgatttc taagaaccga tcaggacgat ggaccatagt aaagcgtcca tttgttttta
    45721 gtagcttact tgaaatatcg atcacttctt ctaacgtcgt atgaatctca tgacgggcga
    45781 ttgccaggtg tttattaggg ttcgttttat ttgttggcag ccctttgaaa taaggtggat
    45841 tacagacgac taagtcacaa gaattatgtc gaacgacaga taagctgtct ttcagatcga
    45901 tggtgtggac agtcatttgt tcatttaaat ggttaagcgc gatactacgt cttgccatgt
    45961 ctgctagacg aggttgaagt tcgatagcgt cgatagccgc ctgggttcgt ttacttaaaa
    46021 aaagtccaac tgcgccattt cctgcacaaa ggtcaacaat cttccctttt ttaggtacac
    46081 gggaaaagtg agcaagcagc acagcatcta aagaaaaaga aaacactttg gaactttgga
    46141 taatcttgat atcttctgcg tataattggt cgattcgttc gtcttcgtga agcatggatt
    46201 ccctccaaaa tgaaaaaaat agtcgtctgt attatactat ttttattaaa aagaaccaag
    46261 gtggcttgtc aaacctctat tttttatgca tactatgacc ataagaaggg aaaggaagag
    46321 actatgtttt atcgatttat gcgcggggta gttcgagtac tattagcctt agttaatgga
    46381 aatgcccgct atgagaataa agaagtttta ccgcaagatg agaattacat actagttgct
    46441 ccccatcgta cttggtggga cccattatat ctggcggtag ctgcagcacc caaggaattc
    46501 agctttatgg caaaagaaga actgttcaaa aatccagttc tgcgatttat attaactcga
    46561 tccaatgctt ttccagttaa aagagaaaat ccaggaccaa gcgtcattaa aacacctgta
    46621 aagattttga aatcgacaaa tttgagtttg atcatgtttc ccagtgggac aagacatgct
    46681 acagaactta aaggcggaat ggcattgatt tccagaatgt ctaaagcgaa aatcgtgcca
    46741 gctgtatatc aaggtccgtt gacacttggg gatctgttca agcgtcagag agtgacagtg
    46801 cgtttcggcg aaccaatcga cttatcagat atcaagaaaa cggataaaga aggtatggcg
    46861 gagatcgaac gacgaacaca agcagctttt gacaaattgg acaaagaagt gaaccctgat
    46921 ttcaaatatg aagtcaaaaa aaaataaaat gataagttta tgcgtgggcg gtcttttgaa
    46981 tctactgtcc tgttcagttc agactttttc aatgaaaacg agggagagac gtcaagtctg
    47041 ccctcttttt gcgttccaat atgtaaaata gaagacaggt gtcatgtaat ggatcaaagt
    47101 gttataataa ttagaaggac attttttgag gtgattgaat gcgtttttta catacagccg
    47161 attggcatat cggaaaaaaa ctacaaggtt atgacttgat tgaagaccag cgtcatgtac
    47221 tatcttctat actagatatc gcgaaagaag aaaaagtaga ggcaatcgtg attgccggag
    47281 acttatatga taggagtgtc ccagctgttg atgccgtaga attatacaat gaattgatgg
    47341 tcgattggaa tttaaaggaa aagatcccga tttttgctat atcaggaaat cacgatagca
    47401 gtacgagact ttcagcgggt acgccatggt tcagttattc gaactatttt ttgcgaacac
    47461 agctagcaga agcatttgaa ccagtcgagt ttaaaaacgt acaatttttc tttcttcctt
    47521 actttgaacc aattgcagcg agattatatt ttgaagatga caatatccga acgatcaagc
    47581 aagcggtagc caaggtgata gataaaatgc aggagctatt tgattcagaa aaaaaacaag
    47641 tattgattag ccattttttt gtaatgggaa gtgtaaaatc tgattcggaa acgaaaatcg
    47701 aagtaggcgg gttggattcg atccctggtg aactacttac accgtttgat tatgtagcac
    47761 taggacattt gcataatcaa gctgctttga gacaggataa tgctcgctat agcggttccc
    47821 cattgaaatt ctctttatct gagatcaacc aaaaaaaagg cgtatggatc gttgacctga
    47881 caacaggcct ggagttgaat tttagggaga tcgaacccct gcatgatatt gaagaaataa
    47941 cagcgagttt caaagaattg ctggatcctg ccttttatca gtccgtcaat cgggagaatt
    48001 acatctatat ctatctcact gatagaacga tcatcccgaa tatgatgaac cagcttcggc
    48061 aaatatatcc tcgtattttg gggattgaac gggcaaacgg tcgagcagaa caaacaaagc
    48121 gcaaacgaaa agtactagaa atgaaagatc ctcaaaaatt agcagaagca ttttttagtg
    48181 aaatgacgga agagcagcta actgcaaacc aagaaaaatg gctcggagaa acgttgcaag
    48241 aaatcatgga aatggaatga tcaagaatga aaccgaaaaa attacaactt aaaaattttg
    48301 gtccttttat caatgagacc gtagattttt cacgtttgac agaagcaccg ctttttttga
    48361 tcagcgggaa gacaggagct ggcaaaacga cgatttttga tgggatcacc ttcgcacttt
    48421 ttggtgaaac ttccggaaga cttcgctctg gaaaagagat gcgttctttg tttgctacac
    48481 cagacgaaga aacaagcgtg actttttctt ttgaacatca gcagatgagc tatgaaatcg
    48541 aacgaaaacc agaacaagtt ttagcaaaac gaaaaggaaa cggaacaaaa aaacaggcag
    48601 ccaaagtaac gttgaccatt tttgatggga aaggaaaaga aataaaacaa atcacaaaac
    48661 gtacagaagc agatcagttg atcagagaac tgatgaatct cgatgcgaaa cagttttctc
    48721 aaatcgtttt actaccccaa ggagaattcc gtaatttttt ggtttcatca agcagtgata
    48781 aagagactgt tttacgtcat ttattcggta ctcaattttt ccaacagttc aatgagcgat
    48841 taaaagaaaa agcaaaacaa cagcaacaaa aattggatca tttagagcaa gagctgcatc
    48901 ttctgcaaaa aaggtttgtt ttagtcaaag aagaggaacc agcagcaggg tttgaagccg
    48961 ttttagccca atggacaaac catcaggcaa tactcgaaaa acaaatcacg gaagaaaaag
    49021 aagctctaac ggaacttcag gaaaaacaaa agcaactaga aaattcctac tatcgttttg
    49081 aaagcttgaa aaaaagttat gaagaaaaaa cgatattaat aaacaagcaa aaggaattag
    49141 ctgctcaaaa agacgaaata gaggaaaaga aacgatggat tgggtattat gaatttacgc
    49201 aacgattagt cgaaccaatc cagcgcctac aagaaataaa agttgaaaaa atagaactgt
    49261 cacaaaaaag tagtgagcaa acaaagaaac tagcgatgac tacgcagaat tatgaagagt
    49321 ggcaaaaaaa tgaagcaaac cgcgtacgcc gacaaacaaa aatcaaagaa gctgctcaaa
    49381 aactgcaaaa caaacagatg atggtgccaa ttgtcgaatc cttacgtagt aaaaaacaag
    49441 acgcagaaca attaacaatt tcattggata aagaagaatt gaatgccgag aagctgtatc
    49501 aaaatctcgc tatctatcag gaaagaactc gaattcttca agaaaattta ctggatcagg
    49561 aaaaactgca agaagatcgt cttgcttttg gacagttaaa atatctagat gaaaagcagc
    49621 aagaaataga aaaacaaaaa gcaaaagaca ctgggtcgct cgcacagata agagaagaac
    49681 tcaaaaaagc aactgaagaa ataacaaaaa atagtcagct attgaaagaa cagacagaac
    49741 aagcagaaca gctgaaaagc caatgggcaa agcagcaaat tgctcgtctt cagctttttt
    49801 tgcgtccagg ggagccatgt cttgtttgtg gatcaacgga acaccccata caacaattaa
    49861 aacacgaaca gccaaataag gaagatatct taaaagtaga acaacaactg gcagaagctg
    49921 agtctgctgt agaaagaact cagcaaatca ttgccaaaag tgaagcacat gaaaataatc
    49981 ttcaacaaca attatcagat ttagaacaaa aaatcatggc aacgaatata caattggctg
    50041 aacaagaaga acacttgcaa caggctttga aaaaggcatt tcctgagata acagaacaaa
    50101 aaacaaaaac aactcttgta gtaatcgaac aatctttaga tagacgaaaa agcaagatgg
    50161 ccaatgaaag aatagagctg gaagaaataa gacaacaagc agaaaagctc gaacaacaat
    50221 tagctgtcta caaacagcag cagacagaaa aaaataatca gctccagcaa ctgcaaggag
    50281 aaatcaaaag tttagaacaa caattgcaag atacggatag taactgttta gaagaggaga
    50341 tacgacgact tgaaaaacag ttagaagaag actcgaatca actagctgtt gagcagaaag
    50401 caggagtgca attgttgcaa gatcttcata cgcttcggac acagcttgat cttcaacatg
    50461 aacaaataga aaaaatagat gaaaaaatag aaaaggaaca agaaagaata gctaaaaagt
    50521 tagaaagcca gtcgtatttt tcaacaattg aagaaatctg tgcatttgca caacagcaag
    50581 cacaatataa agaaaaccaa cgtacagtta cttcttatca tgaagagcaa ttagtgacag
    50641 ctgaccggct gcgtcaatta gaaaatatcc agctaactga gacattccca gatacagaag
    50701 agatcagcca acagttagaa gaaataagac gtatgatcga aataaagcaa gaaaagatat
    50761 acggtttaca agaacaaaaa attagtaatc aaaaaatcta cgatgaatgt ctttcgattt
    50821 atcacacaag ccagacgaaa ttagatgagc ttacgcaatt acagcagctt tcgcaaacat
    50881 tcaacggaga aaatcctaag aagactagtt tggaacggta tgtgcttcag gtttatcttc
    50941 aagaagtgtt acaagtagca aatgatcatc tccaacgttt aacgaaaaat cgttatcaat
    51001 ttgagctagc ggataccatt ggcagttatc gagggaaaac cggattagaa atcaatatct
    51061 atgatgatca ggcaggtatg tctcgaagtg cgcataccct gtctggcggt gagagtttta
    51121 ttgcagcact ttctttagca ttagctcttg ccgatgtgat ccagacacaa gctggtggtg
    51181 tagcaatcga tgcattgttt attgatgaag gatttgggtc attagatgaa gaagctttgg
    51241 aaatggcaat ggaagcttta gaaacgatcg aaagtgaagg aagaatgatc ggcatcatta
    51301 gtcatgtaag agaattgaaa gaacgagtat tacaacaaat aagaattgat acagaaggta
    51361 gcggacaaag caaaatacgc tatcaacttg gataaaaagg gagattggta actatgaaat
    51421 ggagtattcc agaaaaagtc attgcacgtg gacgtcaata cttgcaagaa ggccgagtgt
    51481 tatcggttgt ccctgaccaa gaagaaaacg tgtggcatgc agaagtcttg ggcagtgaat
    51541 tgtatatggt tgatttggat ggtacagcaa aagagataga ttactgccaa tgtccctatt
    51601 gggaggaaca taaatattgt aagcatacag ttgctgtgga actttattta cggtcgaaaa
    51661 atctatctcg tatcatgaaa aaagaccagc caatgctaga accggtaaaa gcaacaagtg
    51721 agggagaaat cattgcaaaa ggtttttctc gtctaaaaac acaaaacaat cctcaagcgg
    51781 tgcaaccttt gattattgaa taccaagtag aaacgattga gactaatcag tatcacccag
    51841 agctttctat tttggcactt tacttgcgta tcggttcatt ggcatcaccg aaaaaaacat
    51901 atatcgtaaa aaacatctat gattttttac aagcttacac agaaaaagaa acttacactg
    51961 ttaataaaca gtaccagttt cgtctagata aacgggcgtt tcaaaaagaa gatcaaaaaa
    52021 tactcacgca tcttgcagga agtgctcaga cgcatcaatt attaggtacg aacggcttac
    52081 aagtcaaagg aaaactagat aaaagatatc tgcttctccc agtagaacaa gcccgcttcc
    52141 tgttagagca gatgaatcag actacacgtt tgcatctgca gataaatgaa caaaacgatc
    52201 aaggaataca attttctgat caaaacaaac ctttatcatt caaagtggaa aaatttggag
    52261 aagattattt gctgcaagca ttgaatgatt ttgatttttt ctttatgcat tatcagtggg
    52321 gagttatcca agggacattt tattctctga cgaaatatca acaaacggtt tatcaaactt
    52381 ggaaacaatt gattcgccga ttggagaaac cagaagtagt gttcaagaaa aaagaattac
    52441 cgtctttgtt taaagagatt atccctctgc tgtcagagat cggcgacgta gctattgccc
    52501 ctgcagtaga agaggaagta gcagatatcc ccgtcgaatt tattttcttt ttccgaaaag
    52561 caaaaggaaa aatccaagct cgagtcgatt tcgtctatgg tgaagtcatc tattcgactg
    52621 acccgaagca tgaggtctcg acagaacagg cttttcaggt gttacgtgac acagtagaag
    52681 agaaacgagt acttgatcta ttcagaatgt atcgctacca gaaaaatgaa actggttatg
    52741 aacgtccatt gccaagcggg gaagaactat atgccttttt ccgcacagaa ctatctgtct
    52801 tccgtcagtt tggtgaagtc agacttggaa gaaaattgcg agagttgtat ttagatgcgc
    52861 ataaacatca accgaaaatc gaagtaaccg agaccggttc atggctcgat attcgattcg
    52921 atgtgacagg gatagaggag caagaaatcg atcatgtcct gcaaagcttg ctgagaaatg
    52981 atgcttttta tacattagaa aatggtcagg ttctttcttt tgattcagaa gaatttcaac
    53041 aaacaagcca agtgttgcaa caattacggg aatcgatacg tacagaagaa ggaacgatcc
    53101 atgtacctaa aaatcaagga ttgatcattc aaaatcaatt ggaaaaaagc aatgctactt
    53161 ttagtgaatc gttccaaacg atggtacaag atctcattca tccagaaaga taccaggcac
    53221 agcttcccaa aggattgaat gcaacgatga gagattatca aaagcaaggt tttcgatggt
    53281 tgaagatgtt aggtcattat cagtttggcg gtattttagc cgatgaaatg ggacttggga
    53341 aaacactaca gacaattgcc tttttacttt cggaaaaaga agaacggaaa agcttctctg
    53401 ctttgatcgt agcgcctgcg agcttaattt acaactggca ggcagaggtg agaaaattcg
    53461 ccccttcttt aagtatccaa gtaatcaatg ggaataaaaa agaacgtgaa gaattactgg
    53521 ctaaagacac agatatccga gtgacttctt atgccagctt aagacaggat ctagctaatt
    53581 atcagtcgca aaaaattgat tatttgattc ttgatgaggc acaaatggtc aaaaatagca
    53641 gtacgaaaac tgcacaagca ttgagagaat tagctgtacc tcagcggttt gcactgagtg
    53701 ggacaccaat cgagaataat ttagaagaat tgtggtcatt gtttgcaacg attatgccag
    53761 gatttttccc gactaaaaca aaattcaggg aacttgccgc agaagaaatt gcgcaaatga
    53821 ttcgaccgtt tattttacgg cgagataaac aaactgtgtt gaaagacttg ccggaaaaaa
    53881 cagagatgaa tctatattct gcacttacag aagaacaaaa aactgtttat ttagcttact
    53941 tgcgtcaaat gcgtgaagaa atcacatcaa tggattctga agcattcaag aaaaaccgga
    54001 tcgggatact tgctggtctg acaagactgc gacaaatttg ctgcgatcct cgtctgttta
    54061 ttgaagatta ccaaggcggt tccggcaagc tcgaacaagt aaaagatcta ctggtagctg
    54121 ccaaagagaa caaacgaaga gttttgctgt tttctcaatt tacagggatg ctgactatct
    54181 tgcaagaaga gttagccgag ctagggatct cgacatttta tttaagaggc agtacgaaac
    54241 cacaagatcg gttaagtatg gtagacgcat ttaatgctgg cgaaaaagat gtttttctga
    54301 tttccttaaa ggctggggga actggattga atctcacggg agcagatacg gtcatcttgt
    54361 atgacctttg gtggaatcct gccattgaag aacaagctgc tggacgtgca catcggatcg
    54421 gacaaaaaaa tgtggtagaa gtgtggcgta tgatctctga aggaacgatt gaggaacgca
    54481 tggattcact ccagcaagaa aaacgcgaac tgttccaaaa agtgatccaa ggtaatgaag
    54541 aacagttgac gaaactgact gaagaagaca tccgcatgat tttaagtgta ggagaaatcg
    54601 aggagtaaga gactcgaaaa agcgttcagt aatgggtaag aaagcctttc taaagaagcg
    54661 attgtttatt tgatttaaaa aatagcaagg aggctgggat tccattttat cccagcttcc
    54721 ttgctatcat ttttttattt ctatttttaa aggttataca aagattgtct tcattgtctc
    54781 taacagctga tcaaaagaag aaatctcgat atcgcaaatt ggagtctgtt caattttgcg
    54841 ttctcgatgg ttgaaccaaa gcgcttgcca gttagcactt tttgcaccta gaacatcatt
    54901 gtcgtaatta tcgccaacgt ataaggtctc ttctggaagc atatgaaatt ctttctcagc
    54961 cagctgaaaa atctcttttt ctggtttttg gaaacctgtt gcttgagaaa tgagcatatt
    55021 gcgggaagga atccaattgt tcaattgaag ttgcttcagt ttttttgtct gatggtcagt
    55081 cggcccattt gtgataattc ctaatgggac attcttttcc ttcaagaaat cgagggtttt
    55141 tttgacctcc tcatgcaagc agatattatc aagttcttcc tcgtaaattt tttgaaataa
    55201 gaggccatct tcttccgtaa tatgagggta gtctaaatct tttagtgatt ggctgatccg
    55261 atgtgctcgc atatattcta gtgtccaatc tcctgccatt acttttggga agttttcatc
    55321 actatgatgg cggaaccgga tatatagtgg atgcatatct gcatctgaaa caagcggaac
    55381 gactcttttg accgcgtttc gaaatggctg ctgctgatca tacatcgtat catcaacatc
    55441 aaacacaact gatttaatca aaattttaca cgcctttcgg ttttcattga ttttttcaca
    55501 aatccttgac tattgtaaca gaaaaaattg tctaaaaaag ctgataaata aaggtttttc
    55561 ttactatatt tctatttcct tgacagaata aggtttttat atttcagttt ttttcactaa
    55621 aaatcgtgaa acacgtcaat aagtttgatg aacaaattaa tcattatggt agaagtcgat
    55681 tgatttattt taatgcaatc tctgtggaat tttcgaaaga tttatttttt taatacagtc
    55741 tcaatataac tattcaaaca taaaataacg ttatttgatt tatttgttta tagtaaattt
    55801 tatttagtaa tcattgcttt aaaatactac gcttatataa cagagaaaat aagcgttata
    55861 ataaagaaca gtattattta aaatgacaga aaacattact attaaagaaa taaaaaacat
    55921 aaaaaatctt aagaaatcgg tttcatttag cttttgagat aaaataatag tacagtttta
    55981 tacgctgttt catatgttta gaaaatttga attagatttg aaaactctaa tttttaaatg
    56041 ataaaaaaag cgagaatgct ggttttactg tatattaacc ttagataact ttcatttttt
    56101 taaaaaaatt atgaaaaaaa ggtagctatt taaaagttga ttatgtataa tagaaacaga
    56161 tgggagatta ttatgatctt ctgttacaaa aaagtgattc ttatcacgaa aaggagtggt
    56221 gcacgttgtt aacattatat acttccccaa gttgtacttc ctgcagaaag gctcgtgcgt
    56281 ggttgcaaga acatcagatt ccatttgttg aacgtaatat tttttcagaa ccattgaaca
    56341 gttctgaatt aaaagcgatt ttacaaatga cagaagacgg aacagaagaa atcatctcga
    56401 cacgttccaa agtattccaa aaactgaaca tggatttaga tgatctgccg ttacaagaat
    56461 tattggaact tgtgcaaaac aatcctggat tgcttcgccg tccaatcatg attgatgata
    56521 aacgtctgca agttggtttt aatgaagatg aaattcgtcg gttcctaccg cgcgatgtac
    56581 gacaattaga attacgtcaa gcacaattga tggctggatt atagaaggat aatacgaaac
    56641 ttggaaaaga
//
LOCUS       DAJSBL010000017        53149 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.017, whole genome shotgun sequence.
ACCESSION   DAJSBL010000017 DAJSBL010000000
VERSION     DAJSBL010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 53149)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53149
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.017"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            277..1602
                     /locus_tag="ONT85_001131"
     CDS             277..1602
                     /locus_tag="ONT85_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HCR2378121.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRS
                     DNTGSGLILASSPEAKKRYGITNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTKSLNLFTTEETRSQRRKKLAQMIQERIKEELGLIA
                     TVGVGDNPLLAKLALDNEAKHNEGFIAEWTYENVPEKVWNIPEMTDFWGIGSRMKKRL
                     NQMGILSIRDLANWNPYTIKNRLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLNIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQINEEN
                     LDKIIDKIRQKYGFTSIVHASSMLESARSITRSTLVGGHAGGNGGIKND"
     gene            1595..1945
                     /locus_tag="ONT85_001132"
     CDS             1595..1945
                     /locus_tag="ONT85_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2378122.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEEIDADNEQRTSQNLAKEQMTTE
                     EIAEVLNAAVIKNRSVSIQKEERNAEGYYPEDIVGKILGSDELGIYVGKDKIHYDEIR
                     HIEFYQDNKWSRLE"
     gene            1942..2121
                     /locus_tag="ONT85_001133"
     CDS             1942..2121
                     /locus_tag="ONT85_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2378123.1"
                     /translation="MNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSFYVEAA
                     AGYHNFRFYIFPLYI"
     gene            2257..2547
                     /locus_tag="ONT85_001134"
     CDS             2257..2547
                     /locus_tag="ONT85_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HCR2378124.1"
                     /translation="MSINTFVYSHPINVYIIKNLGITVEQFCELYAYPQGTVASWITR
                     QRRIKSLPASFVYDLSLASSLNMSDVYEKLLSLEKEYDSFKIKHDKKIKKHI"
     gene            2900..3703
                     /locus_tag="ONT85_001135"
     CDS             2900..3703
                     /locus_tag="ONT85_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HCR2378125.1"
                     /translation="MGDILMARILSVANQKGGVSKTTTSVNLAACLKFVYGKNVLLVD
                     IDPQGNATDNVGFDIDGTNQPTIYEVLKGEADITDAILDYKGIDVLPADIALSSAERE
                     FTQVGSEHRLKRVLQPIKDNYDYIIIDCPPSLGMLTVNAFTVSDEIIIPVEAAYFSLK
                     GLIKLSETIETVKEYTNDKLEVRGVLFTKYNKRYNISKEMTTAATEISRVIGAKIFKT
                     YIRRTITVDEAQAAGSDLINFNKPSTAEDDYKAFTEEYLKEVGDFDGRR"
     gene            3690..4016
                     /locus_tag="ONT85_001136"
     CDS             3690..4016
                     /locus_tag="ONT85_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2378126.1"
                     /translation="MAEDNRSQAAVSRLLGNKNKKNYKPKEVEEIELTKKNISKVIKD
                     QGSSVRTADSIRINPILMSALKYWTTIAESDKSKPDVIEDALLKSIPEEYLIEGYKLA
                     KKQNKI"
     gene            4365..5405
                     /locus_tag="ONT85_001137"
     CDS             4365..5405
                     /locus_tag="ONT85_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HCR2378127.1"
                     /translation="MNDFNYYKSKEIYREKYYQMPKVFFTNEKYMDLSNDAKIAYMLL
                     KDRFDYSVKNNWVDSDDNIFFIFTVEELMKLLQCREGKVSKIKKELEKAGLLKQKRGR
                     VNKRDGKIESMPNLLYLGKPEVTNQDVFKIMEEEDNTDSTVIAKIANTAKPRKINDST
                     VIAKIANTAKSSHIKDSTVIAKIADNLFYSNSIDTNRHIIDTETDQLQNQVLLDNFVD
                     IMQEDSINTFVPENVLNLIKTFSSSYSEAQQTVQTIHNAKKKAEELEQTIVVYEELES
                     YGIDADKGLYMTLLKAYQKQKTEKVNNLQNLIFVYVKNWFVEKAIAAKLANEQRETFN
                     ELPTVSTENWLE"
     gene            6241..6573
                     /locus_tag="ONT85_001138"
     CDS             6241..6573
                     /locus_tag="ONT85_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HCR2378128.1"
                     /translation="MSYTGILSLEDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIS"
     gene            7164..7493
                     /locus_tag="ONT85_001139"
     CDS             7164..7493
                     /locus_tag="ONT85_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2378129.1"
                     /translation="MIQKFAKMSKTAWIEKLENDLSVMRLAPKKFKREIAEKEAILSV
                     LNAVDDKCPVKISFSDIATIIEGIYYGEVDEEISSTKDLLDFLDKNKDTFIVLTDYEE
                     VTTNEKL"
     gene            7480..7821
                     /locus_tag="ONT85_001140"
     CDS             7480..7821
                     /locus_tag="ONT85_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HCR2378130.1"
                     /translation="MKNYDPNSRWGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQKLTDNPIGFGLLPEDDQGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSV
                     EIIEFVADKEE"
     gene            7935..8174
                     /locus_tag="ONT85_001141"
     CDS             7935..8174
                     /locus_tag="ONT85_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2378131.1"
                     /translation="MARPVLEAMDMDTLTKGRYVQLLARGYSPKEVFKSLSKGTDMEK
                     ERLRKEFDYWRTHNGIKDLKPARPVIKRKKKITQD"
     gene            8241..8480
                     /locus_tag="ONT85_001142"
     CDS             8241..8480
                     /locus_tag="ONT85_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2378132.1"
                     /translation="MNYEEIENRKKVSKEMEEKLLKTMKQKHLKRLSVMQYINDMQIT
                     GKEKACLLGSMKNFEQLRRTYVKKSSNCQLLLEVS"
     gene            8566..9237
                     /locus_tag="ONT85_001143"
     CDS             8566..9237
                     /locus_tag="ONT85_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HCR2378133.1"
                     /translation="MRKWLFGFLGVALIVFCSVFGYVSYQKHEGKVFKQNIEKKMPVD
                     QINEHAKSYKEDATNVNNDMSLGQMLSIQKEAIEMGVNKQAFAQIQIPALGLALPIFK
                     GANQYTLSLGAATYFYEDAEMGKGNYVLAGHNMEMPGVLFSDIQKLSLGEVMDLVSND
                     GVYRYKVTRKFIVPEYFKLIDGVPEENSFLSLPKKGEKPLLTLFTCVYTSQGKERYVV
                     QGELQ"
     gene            9290..11266
                     /locus_tag="ONT85_001144"
     CDS             9290..11266
                     /locus_tag="ONT85_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HCR2378134.1"
                     /translation="MAINRYVKKAGMLVGMLGIIASCFQGYQVHAEDVTQKTPPEKVN
                     ITVHKLMYDQGTQLNVDIDGIKNDGYTHDQYPTGVTKYNKADYGDVEFTLGNITDQVL
                     PREDSDLTNAKVDEIVQDVEDKGSNSEYVKNATNKITSAVDENGEITFTDQPAYVNSK
                     GNVYVVYESKSAAGLVTQKAKPMVVIAPMTDKTGSGFLKNIHLYPKNIVSKLSFELTK
                     FGDDGAAQSKQTLLKGAKFELYKGEPGKGTKLGDLVSDDQGKLTATDLTLGKYYFVEV
                     PSEVVVGSDQEPTADQYLLGADARNDAHNKLTFEITNDGVTSDLKASYVNYKAPVIDK
                     TVTNGTGQEHSFQIGDAVNYQGTIHIPTDIAGGTDGITVNGVKSETSPYSVFKWGDTA
                     GQGLSYVAAKANIKVTNKDGSVVLKENTDYKIQNSENGFVIDFIVNNGQVSDTVASLH
                     GQDLQMTYNMYVNDSAAVANPLTNSVDFVYNNNPFNQEEHHEKTKADVVTYGAKFLKV
                     DSGLFGTGIKATPLEGAEFAAKNAEGKYYGGLVDTDKDGVKEAVWVDDVANAAILKSD
                     KEGHFEITGLTEGEYSLEETKAPTGYQKLTEDIAFKVNKNSFKEENRITVKNNQKAAI
                     PLTGSNGFQTYVLISCLLLGAGALSAVVYFKKKA"
     gene            11279..11935
                     /locus_tag="ONT85_001145"
     CDS             11279..11935
                     /locus_tag="ONT85_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HCR2378135.1"
                     /translation="MIIRKPPKRRVMHKLMIFFWLLSAVAVYLIPAVETNVAKFQQQQ
                     RILAIENLEKTDSSLDVAKIELGDPVGVLTIPSIPLKLPIYDGTSDKILENGVGITEG
                     TGDITGGNGKNPLIAGHSGLYKDNLFDDLPSVKKGQKFFIKVNEEQHAYQIYRIEEVK
                     AEELQKNYLDYLMPEKDQDRITLMTCTPKGVNSHRFLVYGERVSFSKEDLKLQKKAKK
                     "
     gene            12031..12858
                     /locus_tag="ONT85_001146"
     CDS             12031..12858
                     /locus_tag="ONT85_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2378136.1"
                     /translation="MNKRKWLFVCVAALFATAAYSQISFADSSTTPTTSQTTDSSTTA
                     TSDTDSSKNTAEETADSSTSSTESAESTSESSSETSEKLQEVEDTQVIKQNIHIQKII
                     SSNRINNQGRQVTQQEGVNGAKFIVYDITNILEEMTQDDNEKNQSVEIIESKLKDRAK
                     KLSYDQLKKVTEGETQTIDDQEGVIDFSIEVPANKKQAYYIVNESSPENISNSEDIVL
                     LTPVSDEKGEFLTDVWLYPKSKKSEPKEEIKKVVSTGVKKNFFDNCWDFFTHLFFND"
     gene            12868..13116
                     /locus_tag="ONT85_001147"
     CDS             12868..13116
                     /locus_tag="ONT85_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2378137.1"
                     /translation="MKIIKMLVFNLSFLAATILCCGLVFSQLSIYPRYQRLFVHFLNI
                     QHASMPLEVINKLLINPIFMASLFLLAGLVTWVLGKKE"
     gene            13159..15258
                     /locus_tag="ONT85_001148"
     CDS             13159..15258
                     /locus_tag="ONT85_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HCR2378138.1"
                     /translation="MENKIHCTTGRVFTLVLFLFLLLFARGTAVQALEKVDPSQIKEN
                     NNLVLYHDSDSLRNIEVTGINGFKILDEYSTKKQKVLAWPKVKDLKSTSKIVAHYQKV
                     GTYLGKEIDCDVTFSNFVAHAGVNQDGLAKTINGKKYYNYLLFDKNIYRGFFMQSINV
                     MNVKLDFKYAGSKTKVALSEPQPEINSKMRSMRAAGIELNDTFLSIYSLNGQYQQRVS
                     WYNDNNESVGERNIDMREFVGYEKMDRLPYFVTTDTACAEYKAPALSTNNLTSKVVGG
                     RAEKAYSSNDHFTDVVGADTYHRSGVTYTLQGASPTFMIGANTNSMMFSFDTALLWTP
                     QPSKPTKEVFNKANTEEAAHNIDKKVIPQGSDVYYHIHQKFDALTVNTMNKYKSFKIT
                     DTFDSKNFDMVSDGKNYDGALWYVEANGTWKKADTGGKLTVSGNTVTYTASDSFLANA
                     ANYGRDYILQLHMRAKNDAKGIAPNVATVTFNNDPNKGTQKTNKVENYFSLKPNKKVE
                     QDGKDVDGRNDGKKGTPTAPLNAGSEVQYLVTQKWHTKGVDIALDHYKQFSIQDPVEA
                     RLTYKEGSARVIDMSTGKDITSEGILTYDSHSRTLKWEASADFLNNNLLDGREIQLIF
                     TAKTPLNEELDIDNQAKVSVESIEKATNVVTIGVKPNLPQVIVPKTGSNHLVLTVIGS
                     ILFLTLASLGYVVLKLK"
     gene            complement(15296..17353)
                     /locus_tag="ONT85_001149"
     CDS             complement(15296..17353)
                     /locus_tag="ONT85_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIVAR domain-containing protein"
                     /protein_id="HCR2378139.1"
                     /translation="MKLFNPKTVLAIGTFAASLSLAGSVDAASTNVMYRLYNPNNNEH
                     FYTSSKKERDHLVKIKWGNYEGPAWEAPTSGGHLVYRLYNKGLRDHHYTASWDEVKWL
                     TKNYGWTYEGPAWRSAEKNNKPIYRLFLPGVKSGSHHYTASWKEVQWLTKDYGWKYEG
                     IGWYGADTSKPKVNKSALEALYTSVKGTKKGDYTDNTWNAFQTALNNAKKVLNDSKAT
                     QKQVDSAKNKLDSAYKGLKKKPVPTVNKAELQALYNRVKSTAKGDFTEESWNNFQTAL
                     SNAKKVLDDSKVSQTQVNNAKNSLDAAFKGLQHKPETPKEYMVTINHIDNQTGQVIAT
                     DQAKVTSGSTYTAKAKAFKYSEGAKDNFSYKVIGNDTQSKTITGNTTITFNYNQVHQI
                     NLLCNNNYKNEGRVDILKEQIINVTHGESVTLTAPSIQGYVLDDRVEPTNSVTLNNVT
                     ESQNYIFNYTRKFNVTVNHVNADTNAVLSSESKTVYEGDSFSTPWKNMTDQNYFLCRN
                     DDPTVTVDKNGQRSINNVDQNKTITFKYKKISLDELRNFMRQKELAWLNDYRQQNGVA
                     PMQFNDIVQQAADIRAKELQVSFSHYRPGGGTFQDLLESLGCYGAKGENISQTGLYID
                     DLLSDDGAIDAMVGWKNSPAHNANLLYNNQSILGLGHNFVVDSAGRIGVDNVFISANP
                     IFK"
     gene            complement(17467..17679)
                     /locus_tag="ONT85_001150"
     CDS             complement(17467..17679)
                     /locus_tag="ONT85_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2378140.1"
                     /translation="MYADKKKIEWLLFDSGKSVRSISIESGVPLTTVSDLANKRSSID
                     NMRFINAMKLNKLAEEIVDINTSTNK"
     gene            17866..18333
                     /locus_tag="ONT85_001151"
     CDS             17866..18333
                     /locus_tag="ONT85_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2378141.1"
                     /translation="MKKKQLWFVSMFAIFFIILSGCSSNKKSEAKEDNSLLQPGTVWK
                     EEVGGLIHNLKIIDDTTWEYSESVWHPDPVQITVKRQKDYEGLERYKIVDSAGIREFI
                     NKPDSLFIVVPYEKDGVKKIIFLGSSKDEKRTKEKLIHDGSQSNKYKLQKTSE"
     gene            18330..18572
                     /locus_tag="ONT85_001152"
     CDS             18330..18572
                     /locus_tag="ONT85_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5415 family protein"
                     /protein_id="HCR2378142.1"
                     /translation="MIASLFICPEGGITVAKAKTPFEKAASKILERRGVSETQWKQEV
                     LEKAQFDLLRGKDKDLEGYIYQREREKLVFDEIIKH"
     gene            18590..18892
                     /locus_tag="ONT85_001153"
     CDS             18590..18892
                     /locus_tag="ONT85_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2378143.1"
                     /translation="MFESITMNFLTQPKVVAAVDIFRALQTAATSLKNNLMIFAAVAA
                     VASIALGGIFFFFGRRGAENGKTHVGYVLLGVGVILAAASIVVSMFSMFGQTPQGL"
     gene            18963..21017
                     /locus_tag="ONT85_001154"
     CDS             18963..21017
                     /locus_tag="ONT85_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2378144.1"
                     /translation="MFFTISGNAKLDKELIRKATKKVGKRTSFLPKTIKYSVIETEEQ
                     LHVLGQVLEQENQSSVKLTLTLHDKEGKIEKAGTEYVFGSLDYQISEGFYPTLIDLIR
                     LDIFKDTDETMSVEESYRNEKILQVLEEELESDQRKVNQVSEVEANEEVPWERPIFPK
                     DESKEKEKGIIDEQADILTHKSIFEEENEVMGQLEVDPFEETMEDEETAEVEVDPVEA
                     NEDPTWTEGDSQEYIEPLISEEVEKNETEIQKATVFDYILDQYPPEHEWMKEKNKLFL
                     NELYSKYQLPQTYQAFLESKEELISQGKGTLLEQLESVQSTNWHELAQEDLLATFEER
                     QQETDQEINEYATEQSSNWEKAKEELNKEEQRVIEEEVQKIKERYEKKRDNAYSLCVE
                     KINNFTQTNQEALSKEKENLLKERIKERKEEFYRGLRTDKLRISKELNEQLNKLYQTT
                     TEVLMEKHEQIQQELQAQIPKWKKEYEIHQQELKEQEEKQRKKEKEREQLELKKREQD
                     LAEQKMAIEKQRLEQEKEKEAAARKERQEQMDTLRQAQLNYLTHPQPVAAVQTPVNNQ
                     ANLTPSPQRSFNGWMVGCIASLSLLLGGGTVFAFNHINQSSQADSVATTASIKEEAQE
                     QARKEYEQQLEELLNRTNQSTTESSMENSKESDSSKAKEGSSDSTVQSNTSESSESNE
                     VK"
     gene            21017..22849
                     /locus_tag="ONT85_001155"
     CDS             21017..22849
                     /locus_tag="ONT85_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HCR2378145.1"
                     /translation="MRVRETTKRKDKRFNLEKNRYLSHFYRYTPSGEELPEKHDKRIS
                     KKIFYSGLVYLPLEYLVTRVPEVFNRLEIVKGSLQGINLFQLQEMPQVFHFDSAGFWN
                     PFTPTTSLGTRLAISTVCVVPAVLMGLKMDMWFRPLADGQKGDNRLMTLKEIQQIYPQ
                     IPETKTTFPGYGGIPITHYKKYWYIQTDTVNTCIVGTSRSGKGQIEVLATIDNLSRAE
                     KQSSMVVNDPKTELYVGSKDELEARGYDVYAFNITDPLQSMSDNPLALIVKYWKRGDI
                     DTATQLTNTFTNTIYYDANASDNKYFNENAQKAVNALIFALLEQADQTGDYSKLTPNN
                     LVELLSEIGGFNYYPDPDNEFKQLNALDEFMNQLPPGNIAKKQYGATKIGSDKAKGNI
                     LSTAITGLNPFTLTKIAKMTSQSTIDYKSVGFPKYLDLKLHESLLNQRVVVEFYHKGK
                     KFHEERVKVGYKGFSEINFDCHLVAGDQVCLKYDHQGKEYVAWYEFSQRVLKDEEGNI
                     VYRKKRGETHLPELEKEAVLTLDEEKSNLLVESIRMHYTDKPMAIFMLTPDYDPSNHI
                     IVSIFLSQLYKELSTQCVKTKGDKCHRRIHFILDEFGNSVTCLA"
     gene            23562..25460
                     /gene="ltrA"
                     /locus_tag="ONT85_001156"
     CDS             23562..25460
                     /gene="ltrA"
                     /locus_tag="ONT85_001156"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HCR2378146.1"
                     /translation="MTNTKGISLKNKQLRHAEYYDMTETFDELHAMSVAKSNFKNLMK
                     FIIDDNNIFLAYRNIKKNKGSATAGYDGVTIKDIEKLACGDFLTIIKRRFKDYRPRKV
                     KRVEIPKPNGKLRPLGIPSIWDRIVQQCILQILEPICEAKFNKHSYGFRPNCSTENAI
                     ADCLARINQSKMNYVVDIDIKGFFDEVNHTKLMRQIWTMGIRDKQLLVIIRKMLKAPI
                     VMRNGNVVFPTKGTPQGGILSPLLANICLNEFDWWIANQWEERTVKELKHHYRKNGAW
                     DKGNIYSKLRSHTSLKEMYLVRYADDFKIFCRTGEEAQRAFYATQKWLRERLKLPISM
                     EKSKVTNLNKAESEFLGFSIRLHKKGKKKVAFTYVCRKAEENIKNKLKQQIKKIQYAG
                     AKKNRHRAIGDYNMMVIGIHNYYRIASHCSVNFTEIYRSVRTVIEARLKARGMTKNGI
                     YGQDHEGYRPYLKSKQMRYIEGFPILPIGYIKKRTPLNKRRNLNKYTKEGRKLIHQEQ
                     QAIESWKIKYLVKGFSNNKKYTVEFSDNKISKFISQKGKCAITGNELLMEEIECHHIN
                     PYHLSKDDSYKNLVIISREIHRLIHATSEETILKLLRLLKLNHYQIRKLNTYRKKAAN
                     FPISIKMN"
     gene            25577..26377
                     /locus_tag="ONT85_001157"
                     /pseudo
     CDS             25577..26377
                     /locus_tag="ONT85_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
     gene            26374..28905
                     /locus_tag="ONT85_001158"
     CDS             26374..28905
                     /locus_tag="ONT85_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2378147.1"
                     /translation="MIIGGIFSGIADWWSGLFGEGDANPKTVTKVLQDYGNYLQYQDV
                     FSWICNTFLWGLIKGLYNLNTLLEKTIYQSFDLKDLLNAAGVNELFQSLISKVLALFC
                     VVTLIYLGLKFFMSKHPPKVKNILVNLFMAMILIFGGSSLIDEGLNISKSFYGDVTAA
                     NKADKTGSPAFQIIQNNVIDVSTLLATDPNKVESLPTDKRNALTAKNFKTANINGIIT
                     PKQAKEMADKDKSGLSDLQKDRLRALQYRLELDDNGKEIPVQISDEGLAKYVYTSGYR
                     RYISSPGIILAGESSLAVAYLFILFTIVTCIIELVFKKFYLVIAASTDFETGQRMKSA
                     IEDISQSFLLLAFTILELRIYTLMLSGIGDLHAAGKINGFLYVVALIVLTIALFKGSQ
                     SVTKIFGVDTSLKNGSNSLLSMFALGNIAKNVGSGAKNLASAGKSGLSHLNDIRKNGF
                     GHRKDVTPDNGEEQSDDKATTVPNSKKTVSEMFGKAKNGVSKAAEGLGYVNERGLKGT
                     AEDLTEKAKDTLEDKLDGTRAKELAEAVKNPSEALEKQKEKASEVKEGLKDAYGEGQV
                     NAAVKANTTTEKENQEGIVIPSVKDHQEALHSNENSIEEKNSIEERNSEEGKNGQLPK
                     TDPEQYRYKPNSDLENEKRISDLSKVNQEDKAENEGQLPKTDPEQYRYRSVNSENEKR
                     ISDLPKRNQEVQTDEDSIVLPNDNGIEVGTEKSTKIGLPKAQNLPMKDSRLNQFKDND
                     QSFVHQSPAGTVQMKPSRLPENGNLTTVSYNLSESGETGNASVQNVVTREAAPKPIQN
                     VHTGYKPANVAFTAPTKINVTRETKNRNNALKEQLLRDIKQDIKP"
     gene            28955..29287
                     /locus_tag="ONT85_001159"
     CDS             28955..29287
                     /locus_tag="ONT85_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2378148.1"
                     /translation="MDKYINMKNPPRFSGEWKFFAFFFLDLLTLGGCFFWAKMVNNLV
                     HLPLFFALLNYGLWIAIALVIVWRPYNNPGQRQIFAIIESIFFADKNHYHALDPNRNY
                     HGTYKEDE"
     gene            29288..29881
                     /locus_tag="ONT85_001160"
     CDS             29288..29881
                     /locus_tag="ONT85_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2378149.1"
                     /translation="MRVKGQNSVERKSILQFGKPEERSILDLFPYKSCTEEGVLITRE
                     DHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYVPTIKLVSLTTETDLTEQIVQK
                     RQLLDKNRMEQSLGRNLQRLRKYEKKLVEEIQELKRAEQERPDLSFFFVIEGKTIKDL
                     QTRNRQLMRSSGILGLKPLYKKELIKILYRMNNMNDE"
     gene            30044..31738
                     /locus_tag="ONT85_001161"
     CDS             30044..31738
                     /locus_tag="ONT85_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HCR2378150.1"
                     /translation="MLEAIKTAFSPQNDQKVLDPLKQLGNREIEILKSKGYDLDFLKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIENLKGMDYRAVTYLHKTKRDWQSMFTMFDEQAEWFGNIKG
                     QSLPSLNIGGGFPFHHQTLKDPGGTQYGETMTGGPFVWNATYKNSVRLSTNTLLLGMM
                     GAGKSTVLKMIAEAHLAAGDFIWGFEKGKDFIPFLKEYNGIMVRLDGSDGMINPLEIF
                     ATRTYDEASSLYDDGSVKDLKINEAASYQTHLDKVVYQVQLVSPQLKGTMKAEFKTYL
                     NRFYEEYGTVPRGFTSSNSRSNTDTQVTGKDPEAYPTFKEFLDFLGQLELPGASQEKK
                     NRKEEMESIVESLCETYGMIFDGHSTIRHLDQQQLVFFDIDSITGLDRGVQQCQMYMA
                     MTLIWNQALIQGRRQRRLIKEKQMEEQDFKCFLAMIDECHNGATCSAVKSCGLQVV"
     gene            32426..34303
                     /gene="ltrA"
                     /locus_tag="ONT85_001162"
     CDS             32426..34303
                     /gene="ltrA"
                     /locus_tag="ONT85_001162"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HCR2378151.1"
                     /translation="MTKETSPKDRKLRHAEYYGMTEIFDKIYSKSLKGDNFKNLMQII
                     ISDNNIVLAYRNIKRNHGSVTRGEDGVTIKDIENLNRNEFVEIVRKRFAHYNPRKVRR
                     VEIPKYNGKLRPLGIPSIWDRIAQQCILQVLEPICEAKFNNHSYGFRPNRSAEHAIAD
                     CMARMNQSHMEYVVDVDIKGFFDEVNHTKLMRQIWSMGIRDKQLLLIIRKMLRAPVVL
                     PNGKLIYPTKGTPQGGILSPLLANINLNEFDWWIANQWEEKDAKELKHHFRKNGAWDK
                     GNIYHKLRKSTTLKEIYLVRYADDFKIFCRNYNEAKKVFYATKMWLQERLKLPISEEK
                     SKITNLSKEESEFLGFTMKLVTKGNKKVVNTHIGTKAQSMIKVKLKKQIKRIQKSPNS
                     KDTFIYIGVYNSMVIGIHNYFKIATHCSVAMRKIARELRPSFEIRLREQGISKEGQYG
                     KYEGYKPYLRSGQIRYIRGFPILPVGYIQHRHPMCKKRNINKYTEEGREAIHKKQQTV
                     EEWKISWLCSNPVLSKRATIEFADNRISKFIAQKGRCAVTGEELILSEMHCHHIIPYH
                     ESKSDSYENLVIVTEEVHRVIHATQSETIEELLKYLKLNPKQKEKLNELRLKVGNEEI
                     S"
     gene            34434..34811
                     /locus_tag="ONT85_001163"
                     /pseudo
     CDS             34434..34811
                     /locus_tag="ONT85_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
     gene            34811..36016
                     /locus_tag="ONT85_001164"
     CDS             34811..36016
                     /locus_tag="ONT85_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HCR2378152.1"
                     /translation="MENQNESLIKQYVKRRAKRRLFLWLFGTSAGLITILITVFVTLF
                     LILAAGSIDNSDSDSSSGGEAFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYK
                     LLPSVILSQYGYESAFGTSASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYMKF
                     PNSKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGRGGYAAAGITEDSPYYTGCMS
                     IITSNKLTEYDEFAIKHWGEGGNNNGTITGEWTNPFPGSSLDKSSFSGGQLFGTNPGG
                     EFRPNGFHDGLDFGSVDHPGSEIHAVHGGKVVYVGNPGISGLGACVIVINYDGLNMVY
                     QEFANSTGNSRVKVGDQVKVGQVIGIRDTAHLHLGFTRMDWRQAQGHAFTDDGTWIDP
                     LPSLNSSKK"
     gene            36032..36676
                     /locus_tag="ONT85_001165"
     CDS             36032..36676
                     /locus_tag="ONT85_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2378153.1"
                     /translation="MENKTLKIVLTAAISVSLIGNIALFWQLKEEQETNTFKIEEVAK
                     NASEKLKKNSSEAQAKIDKLNQTINEKNQEIDQLKQQDGTKAKEEVTEAQRKTAEEFG
                     QLAIDKSLSRNELTEKLKDVATQEVIDKLSPADDDHQHDDYGSYSFTLGDVQTYAQTS
                     SVKEEQNFVVFVDYTIGNPQFKDVKPQKIKGGLTVTEKQVDGQWKVIDFSYFTR"
     gene            36687..37493
                     /locus_tag="ONT85_001166"
     CDS             36687..37493
                     /locus_tag="ONT85_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2378154.1"
                     /translation="MSKKKGERQRFKKRKSYLQWLGIFMILGFLILASPIYTNQKVDI
                     PDEVSTNAFLNTKELTMVSKEKGANQQLVFEFAIDDEDQSLLKELANLNYRVEVKTAK
                     GDYQAIRAKVIKVSDDYFAVKLTHVPEEYIAMRLTIIPEKIDPKVDMQEPQDLIYYVH
                     EDKVKDQVKDEDYEQHAINYKVKGYKKEQKAAQKQMESFQATIDLNEELVKKLKDQLP
                     YQVADDQENTENKINSYQQEIETSKTQLKDQEEIIQKLQEKIDRMTKEKI"
     gene            37515..37745
                     /locus_tag="ONT85_001167"
     CDS             37515..37745
                     /locus_tag="ONT85_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2378155.1"
                     /translation="MKRTNLADKDTFAEQKVLIKAYLKVVKAKEICREVNLSNTTISE
                     LKNGRKNLDTLKFRSFEALYVAAKKYFTADNK"
     gene            38291..38566
                     /locus_tag="ONT85_001168"
     CDS             38291..38566
                     /locus_tag="ONT85_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2378156.1"
                     /translation="MDLLIRNIEEKYINKIDKRCEELTVKTGKKWSRNQYLKVLIEND
                     FDHALLNYKKDQFDRLLEKFVDIQTYNTKTLEEYIATNNQLIGLLIE"
     gene            38608..39030
                     /locus_tag="ONT85_001169"
     CDS             38608..39030
                     /locus_tag="ONT85_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2378157.1"
                     /translation="MAKATRKHVYLYDMHIEVLDEIISTHEDISNYSEAVRHLCMMYD
                     PKIEEKINPIQIKLNAMGKEISILTEMVSEFADLHLKDTGILIGTNSTVYQEAEERVE
                     GKIKSNQTKKYSSKLKKPVNESVHTRVDPFDPTKILKR"
     gene            39049..40641
                     /locus_tag="ONT85_001170"
     CDS             39049..40641
                     /locus_tag="ONT85_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2378158.1"
                     /translation="MNKSPAVILTCQFTLPNKKSFSTYIDYMTREKALEEMERRTPEE
                     EQELQRIKNALEDFYIPEGETFSESKTDKEFSETSNEAQSLMEHGINFDKLEEQDFTK
                     YLSYMTRHYALEQKKELTQREQKEYQRLNQAMKKYQTEPHKKASGNLPGVFSMASDEV
                     KGSDLKEIKQIFQRGQQKGSIIYQDVISHDNSYLEKMGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKENTRKPYTEIENGVSYIVPKG
                     KRRQATLDAMKTNYVHELERFSYEKDRMIGMEKRNLLTRKSDLRNTLTKVVKEPARYD
                     RHAMNLLNEVYKTLPEKRSEWNYGDENRTKLSPFTRGKLDELTKYVLTKEPGYQEYIQ
                     ISKALKEEAKSLYGESKRETKNAEKNALFDLKKRTGNAILTELKRQDSQIKPLIESVN
                     QMQSIYNERSFRTLKQLPTNEYKYSYQEFVKRRKQFQQRVVTQRSLRQLNRTINAHEE
                     KYHASKAYEEQQRRIQMEQERQ"
     gene            40653..40988
                     /locus_tag="ONT85_001171"
     CDS             40653..40988
                     /locus_tag="ONT85_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2378159.1"
                     /translation="MKQYLDQWKVIEGSLKEERIEQLPDCLEKEHLFQISEMLRNEQF
                     DPNQFLVVEYPATGVYCCNHVKGEKYFIIQEYEGKLAPYYTTWEINEEGINNFPCESI
                     EESISLTEC"
     gene            41135..41329
                     /locus_tag="ONT85_001172"
     CDS             41135..41329
                     /locus_tag="ONT85_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2378160.1"
                     /translation="MTQTTIGVVVEKSRDNLVFVTEIQTGRAFVVTDKAAKAYQNGNI
                     LTLNLTTKMFVDAVENYPFV"
     gene            41361..43733
                     /locus_tag="ONT85_001173"
     CDS             41361..43733
                     /locus_tag="ONT85_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2378161.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNNQFQKLFDHQKLTNGQ
                     PMNDKRNGRSWFNQQEKIENKIQSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIENIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKEKSEKGKENLLPEVQAI
                     IDSGRVTQWKKNPTIYFLKGYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     TPTDSVKEKETDHFYRVEFNENDPAKGLKSYGGEIVTLELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNHPIYLELARLCEQSPEKEQEQTVKNEPISKV
                     QNEIKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DQTGQTELQVGIQAVAKNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     ATWTKNSQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTVETAEQKNIVPVGSLWIDKKDGKVLEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNPFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            43794..45254
                     /locus_tag="ONT85_001174"
     CDS             43794..45254
                     /locus_tag="ONT85_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HCR2378162.1"
                     /translation="MTEKKMSLIDRCKQMDIVDFARNNGLAVANKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDPKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPDWLIDYAEKEGLIAELKPKHERQNF
                     LVRDDRLDHAVAFLWKDPQTKETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQLKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPREYKVI
                     YEEVAKEEKVTPEAIMAIHKTENNLQLTNQLVSAHKVNASFGQQLSVNDSIEVISLKD
                     ICREVAKELKDCERSNGTYDFDRFYQGKGDINSQILFSYKAEQYYKGYKNHEHEFVPE
                     VKKDWNDQLKHEIHQQEIRKQKRAMLFQQGRQQERE"
     gene            45265..47469
                     /locus_tag="ONT85_001175"
     CDS             45265..47469
                     /locus_tag="ONT85_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HCR2378163.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFSGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFNVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQAGADQGKTFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAQVTAQNGIFDAKLDPNETQATQEAFEAYLKEKGVQVGKQPGTILEVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EHAYLLDHLDEYKHFLKAESFPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTEATYEK
                     LTPLQQAIYDQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAVGKVPVDLGWKKLFGK
                     ESDRKEKEEEPLLPKVTKGETVTVDLQIFEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGEQKGTQATFLANIQKFVSHLLEVVPGQIQSTDFGSTLQEVKA
                     ASEKQEATRHLGVCPKCREQEVLLYQNVAACTSEACDFKLWTTIAKKKLTATQLKEII
                     QNGRTSQSVRGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNYKKRTRRTTK"
     gene            47485..47634
                     /locus_tag="ONT85_001176"
     CDS             47485..47634
                     /locus_tag="ONT85_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2378164.1"
                     /translation="MRNTTHKQQEIENQKAKIRLEQEKLRANKRRLQQLKNQQRIQKE
                     IELDR"
     gene            47710..48303
                     /locus_tag="ONT85_001177"
     CDS             47710..48303
                     /locus_tag="ONT85_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HCR2378165.1"
                     /translation="MQDKLKELLAIRDGNLSMKEARKFGIAATTVQRLVNQGKLIKVD
                     RGYYVEDGHGIDDLFWLQQRFSRGIFSHETTLDIYQLSTNMPKFIHLTFPHGYNVDRS
                     ITKEQQLQFHFVKKEVYELGKVEGTSFQGNPITMYDKERTLCDIWNPRYEFEYEMKLE
                     ALKEYMEDPLRDPSKLRDYMTKLHVEKEMKYHMQSLY"
     gene            48316..49215
                     /locus_tag="ONT85_001178"
     CDS             48316..49215
                     /locus_tag="ONT85_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HCR2378166.1"
                     /translation="MTPTQFAAKTRNLAKSTGLNAQLVQRHFMMDRLIEQISESKYKN
                     DFILKGGFLLGSKYGIDRRTTIDIDTTFRNSKLTEEKLKSIFNDLTRKPTKEGIVFSI
                     QGMKETREADFYPGFQIKLVANLEKVRVPFKMDVTTGDSIYPEVDTHYHQLMFEDKKV
                     EIPAYPTEQIIAEKLSATFSFGTDNSRAKDYYDLFTIPKLENIDTKELYKSVRNTFTK
                     RGDTKPLSFYYEREMDKIRGNDYLEKQWEKYRSVNTFANSISFTDTVNEIDHLMKSVI
                     KIENQERKRQFKRSQQMNQEMER"
     gene            49218..49703
                     /gene="ssb"
                     /locus_tag="ONT85_001179"
     CDS             49218..49703
                     /gene="ssb"
                     /locus_tag="ONT85_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HCR2378167.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRAHADQSSSSPSPTTFEQRDVATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
     gene            complement(49844..50047)
                     /locus_tag="ONT85_001180"
     CDS             complement(49844..50047)
                     /locus_tag="ONT85_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HCR2378168.1"
                     /translation="MIKTVKFVTVAIRLKVRWCVCAYFQNLTERRSLLPVETALGLMI
                     SFGSLIATIIFGILAVVNNNQKK"
     gene            50122..50382
                     /locus_tag="ONT85_001181"
     CDS             50122..50382
                     /locus_tag="ONT85_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2378169.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIESMQIRNPDHFTRSMGI"
     gene            50819..51025
                     /locus_tag="ONT85_001182"
     CDS             50819..51025
                     /locus_tag="ONT85_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HCR2378170.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYRLYQC
                     PTCGKKYTLSYGDYVLIDEKVKKR"
     gene            51025..51276
                     /locus_tag="ONT85_001183"
     CDS             51025..51276
                     /locus_tag="ONT85_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2378171.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEALLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGYELHYQENMETYEIEYWLTKLEN"
     gene            51291..51728
                     /locus_tag="ONT85_001184"
     CDS             51291..51728
                     /locus_tag="ONT85_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2378172.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKEKLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNGKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYAKFAQIKKAKAMASDWLDTFENIEKNGAFYTIESEEKDNE"
     gene            51721..52428
                     /locus_tag="ONT85_001185"
     CDS             51721..52428
                     /locus_tag="ONT85_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2378173.1"
                     /translation="MNKVVYQLTTEDVSQFLTNKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYMESETSQTKVTVSHNRLDVYKRRPDTLKKGAELLRLETEKYIMNVYLERS
                     ELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKDATVDDYYLVFTRFLT
                     CGTESIVLMGMDYELTVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKRMPYQRFERKKGQ"
     gene            52809..52988
                     /locus_tag="ONT85_001186"
     CDS             52809..52988
                     /locus_tag="ONT85_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2378174.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESRIMCIALDLL
                     IAQEKAGFEVPALKK"
BASE COUNT        19630 a         8355 c        10745 g        14419 t
ORIGIN      
        1 tcaaatgttg taaggatttt attcatttgt taatattaaa gaacggaact ccaaaaaagt
       61 ccgtactcta atttgcagtc tctcactctt ttagagtgag agtttttatt agattttcaa
      121 attaattagt aaagtgatag aataaattga tcatacaaat cataagaaga agcagggtga
      181 tagcatacct atgcttcttt ttttatgtat ttagctattt cttctttaca acatcgaaca
      241 tatgtttgta taatgtggaa gaatggagtg ttatttatga tggattattc aaaagagcca
      301 gtaaatgatt actttttaat cgatatgaaa agcttttacg caagtgtcga atgtatagaa
      361 agaaatttag atccattaac aacagaactt gttgttatga gtcgatctga taatactggt
      421 tcaggattga tattagcttc ttctcctgaa gcaaaaaagc ggtatggtat tacaaatgtg
      481 agtagaccac gtgacttacc acaaccattt cctaaaacac tacacgttgt tccaccacgt
      541 atgaacctat atatcaagcg aaatatgcag gtaaataata ttttcagaag atatgtggct
      601 gatgaagatc tactgattta ctcgatcgat gaatcgattt taaaagtgac aaaatcactg
      661 aatcttttta cgactgaaga aacacgaagt cagcgaagga agaagctcgc tcaaatgatc
      721 caagaacgta ttaaagaaga gttaggtttg attgctacgg taggcgtcgg cgataatccc
      781 cttttggcta aattagcctt agacaatgaa gcaaagcata atgaaggatt tattgcggaa
      841 tggacgtacg aaaacgttcc tgagaaagta tggaatattc ccgaaatgac ggacttttgg
      901 ggaattggtt ctcgaatgaa aaaaaggctc aaccaaatgg gaattttgag cattagagac
      961 cttgctaact ggaatcctta tacgattaag aatagattag gtgtgattgg attacagctg
     1021 tacttccatg ctaacggaat cgatcgaaca gatatagcaa tcccgccaga accgacaaag
     1081 gaaaaatctt atggtaattc acaagtgtta ccacgagatt atactagaag aaatgaaatc
     1141 gagctggtgg taaaagaaat ggcggaacaa gtagccattc gtatcaggca gcataattgt
     1201 aaaacaggat gtgttcattt aaacattggt acgtcgatct tagaaacgag accaggattt
     1261 tcacaccaga tgaaaatacc tatcacagat aatacaaagg aattgcagaa ttattgtctt
     1321 ttcttgtttg ataagtatta tgaaggacaa gaagtaaggc acgttggaat aacctatagc
     1381 aagcttgtgt acaccgattc gcttcagctg gatttattta gtgatccaca aaagcaaatt
     1441 aacgaagaaa atttagataa aattattgac aagattcggc aaaaatatgg ttttacgtcg
     1501 attgtacatg cttcttcaat gttagaaagc gctagaagca ttactagaag cacacttgtt
     1561 ggtggtcatg cgggcggaaa tgggggaatt aaaaatgatt gattacaatg atcgtggacg
     1621 tttaaagtgg gcagggtttt atttatctga tcatactgaa gaaattgatg ccgacaatga
     1681 acaacgaacc agtcaaaatt tagcaaaaga acaaatgacg actgaagaga ttgcggaagt
     1741 attgaacgct gcagtcataa aaaatagatc agtatctatt caaaaagaag aacgaaacgc
     1801 tgaaggatac tatccagaag atattgttgg caagatctta ggtagtgatg agctggggat
     1861 ctacgttgga aaagacaaaa ttcactacga cgaaatacgt catatcgaat tttaccagga
     1921 taataaatgg agtcgattag aatgaacatt atccagcaat atgagctaaa gtatataacc
     1981 tttgatcagc tatcagaaga gatttggggt tatggccaac gtttgataaa tgaggttggt
     2041 gttgagcgct tctctttcta tgtagaagct gcagcaggtt atcataattt taggttttat
     2101 attttcccgt tatatatata aggtatttta cggattctat agagttatct ctatgaatcc
     2161 ttttttgttt ttacaaatag gagtagatac tagaataatg cgtgtgttaa actattattg
     2221 caaacgcaat aataaaagaa agtaggctta cgcaccatgt ctatcaatac atttgtttac
     2281 tcgcacccga tcaatgttta cattatcaag aatttaggaa tcactgtaga acaattttgt
     2341 gagttatatg cctacccaca aggaacggtt gcaagctgga taacccgaca acgtagaata
     2401 aaatcattac ctgcttcatt cgtttatgat ttaagcctgg cctctagttt aaacatgtct
     2461 gatgtttatg aaaagctcct gtctttagaa aaagaatatg attccttcaa gattaaacat
     2521 gataaaaaaa taaaaaaaca tatataatat caatcacaat tgtataatat tacgatatct
     2581 taatatcatg atatcgtaat attgtttttt tctaagaaaa tgcactataa gaagggaatt
     2641 attagtaaca atatagacag tttaataatg tttaaattat tctagttgct gctcgcaaaa
     2701 catatttctt atgattaata aaaaatttta aagtaataaa aagtgattat tttactttcg
     2761 aataacatat gaataatctt tatatccgaa ccgatatata tctttaaaac aaaataatca
     2821 cacaatatta caatataatg catatacgat attgcaatat cacaatatta tgatataatt
     2881 atattataat ataagatgaa tgggggatat tttaatggca agaatattgt cagtagccaa
     2941 tcaaaaaggt ggagtttcta aaactacgac tagcgttaat ttagcagcat gtttgaaatt
     3001 tgtttacggt aaaaatgtac ttttagtgga cattgatcct cagggaaatg ctacagataa
     3061 tgtaggtttc gatatagatg gaacaaacca acctacaatt tatgaagtat taaaagggga
     3121 ggctgacatt acagatgcta ttctggacta taaaggaatt gatgttttgc cagccgacat
     3181 agccctttct tctgcagaga gggaatttac tcaagtagga agtgaacaca ggcttaaaag
     3241 agtacttcaa ccaatcaagg acaactatga ttatattata attgattgtc caccaagttt
     3301 aggaatgctt acagtcaacg catttactgt ttctgatgaa attatcattc cagttgaagc
     3361 tgcgtatttc tcattaaaag gactaataaa gttaagtgaa actattgaaa cggtaaaaga
     3421 atatacgaac gacaagcttg aagtacgagg agtcctattt actaagtata ataagcgtta
     3481 taacatttct aaagaaatga cgactgcagc aaccgaaatt tctagagtca ttggtgcaaa
     3541 aattttcaaa acttatattc gtagaacaat aaccgttgac gaagcgcaag ctgctggatc
     3601 agatctaatc aattttaata aaccctcaac agctgaagat gactataaag catttacaga
     3661 agaatattta aaagaagttg gtgattttga tggcagaaga taatcgcagt caagctgcag
     3721 taagtagact actaggaaat aaaaataaaa agaactacaa accaaaagaa gtagaagaaa
     3781 tcgaattaac caagaaaaat ataagtaaag tcataaaaga tcaaggaagt agtgtaagaa
     3841 cagcagatag cattcgtata aatccaattc taatgtcagc actcaaatat tggacgacta
     3901 ttgctgaatc agacaaaagt aaaccagacg ttatagaaga tgcgctatta aaatccatac
     3961 ctgaagaata cttgattgaa ggatataaac tagcaaagaa acaaaataaa atttaattta
     4021 cattcaatat cacgatatca taatatcgtg atattttttt tataaaaatc agtagtatca
     4081 aagctgataa gtcgcttatc ttcatattgt aatattacga tatcgtgata taatgttatt
     4141 gtaatatcgt gatatagtta ccatgagctt aaaccctgaa atagagcggt ttgctttaat
     4201 ttatttttta aggtaaacta aaaaaagata aaaaaagact actgctttaa caaagtttgg
     4261 cgacttatca gcagtagaaa aatatatagt ggatagatat atttgtttaa ttcattgtaa
     4321 catagttttg aacaaaacgc tatcctacag aaagaggatt ggctatgaat gattttaatt
     4381 attacaaatc aaaagaaatt tatcgtgaaa aatattatca aatgcctaag gtatttttta
     4441 ctaacgaaaa atacatggat ctttcaaatg atgcaaaaat tgcttatatg ctacttaaag
     4501 atcgatttga ttattcagtc aaaaataact gggtagattc tgatgataat atattcttca
     4561 tattcacagt tgaagaatta atgaaattgt tacaatgccg cgaaggtaaa gtatctaaga
     4621 tcaaaaaaga attagaaaaa gcaggtcttc tgaaacagaa gagaggaaga gtaaataaac
     4681 gtgatggtaa gattgaatct atgcctaatt tattgtactt aggcaagcca gaagttacga
     4741 atcaagacgt ctttaaaatc atggaagagg aagacaatac tgattccacc gtaattgcga
     4801 aaatcgcaaa tacggcaaaa ccgcgaaaaa tcaatgattc taccgtaatt gcgaaaatcg
     4861 caaatacggc aaaatccagt catatcaagg actcaaccgt aattgcgaaa atcgcagaca
     4921 atctattcta ttctaattct atagacacta atagacacat aatagacact gaaactgacc
     4981 agttacaaaa tcaagttttg ctagataact tcgtcgacat catgcaagag gacagtataa
     5041 atacatttgt accagaaaat gtattaaact tgataaaaac attttcttca agttacagtg
     5101 aagctcaaca aaccgtccaa actattcata atgcaaagaa aaaagctgaa gaattagagc
     5161 aaactattgt cgtttatgaa gaactggaat cttatggaat tgatgctgac aaaggattat
     5221 atatgacttt actgaaagct tatcaaaaac aaaaaactga aaaagtaaac aatttgcaga
     5281 atctaatttt cgtttatgta aaaaattggt ttgtcgaaaa agctattgcg gcaaagcttg
     5341 caaacgaaca aagagaaaca tttaacgaat taccaacagt tagtacggaa aattggttag
     5401 agtagttgat aaaaagcaat tactataaaa atgatcaatt ttaaattaga acttttttat
     5461 gattttaatt ttattacaaa tattacaaat aaatagttag tattccggag tttttagatt
     5521 aaaagtttat gaaaacaatc acaatcgtag gttttaaatt cataatcgtt agatatttta
     5581 gttatttttc actcaaaaaa atagggaact agtgtttgca tatgtgcttt tttgagttat
     5641 aataattcat gaagagaggt aattcactta tttccccaag ctgaaaagcc tgatattact
     5701 tcttttcagg cagtgctgct aatattcaat tacacttttt tgtatatttt tagttgtttt
     5761 aaatttaatt gtctgctatg cttgtgatga attgattttc gttctgcact tttggacgat
     5821 aaatttggac aattgaaaat acaaaaaaaa gaaaggccct gtgagactcg gaaggctggt
     5881 aaccttcgtc gacgtccaca ggtggcatta agatcactcc cttaatgcgt ttacctttca
     5941 gtgttacggt attacccgta acggatttag tataacagat attcaaaagt gataccactt
     6001 tttttggctg tgttaaggtt aaaaatacgt gatattacga tgaatctatg cctaagcata
     6061 atgttataaa aatgtccgtg tggctctttg ccgtatgacg aacgctgaaa ttagagggaa
     6121 cgcatgtttg gatgcgttcc ctcttttttg tttgtcatga acgaaaatca attctctatt
     6181 tttgaaaaat aacaagaaaa acttgttaaa aatatttaaa gtaagtgggt ggattatttt
     6241 atgtcatata caggaatttt gagtctcgaa gatatttgtc actacggtaa acggtgtacc
     6301 gctacagaaa agatcacgaa gaaactatca actggtcaaa gtaaaaccgt tgtgcagtgt
     6361 aagaaatata taatccagaa agacaaagtc tcagaagaaa tgatttatta cattgggaaa
     6421 caaaaacaaa taattttaaa agatcctatc cctttaaaag agctatatcc aacaatcaaa
     6481 catgtttatg accaaaacgg tgtactgatt ggtcgaagaa aaaatggagt cttgcgttgt
     6541 accgcaaaag ggatggggcg tttaatcagc tgaattgatc attctcataa cttgtatttt
     6601 atcgtttttc gcttgcttga aaatattgtc gagacttcta aaggaactta gaatcactag
     6661 aaaaataagg agtagacttg atctatcaac aattcctttc ggggggtgac ggggtaggtt
     6721 tttacgattg gtaacgtaaa aaattggtca atcgagtaaa tgcccttgtt tggatagtgg
     6781 tgaaatgaat ttgtgtaaat aatatgcaca gagtaaacaa gagggaattt agaagttaga
     6841 caagttagaa tagcgcctag attaatcatg agcagctact aagggatcta ggctttgacg
     6901 caaactcatg gaataaggag taggtcttct cgtaatgggg aggacgcaca agaaattgtg
     6961 tcgtgtgctg cagtacatgt agatacttgc gaagacgttg atctttggat caatcgagca
     7021 tttaccgttt tctactaggg tttctcgttt cggacgccat aaacgagtcg atttatttta
     7081 tatatttttg agtgaacgaa aaacgataaa aatattcgtt atgaagtaaa actaatttag
     7141 tagatagcaa aggtggtaga ggaatgattc aaaaatttgc gaaaatgagt aaaactgcat
     7201 ggattgaaaa gcttgaaaat gacttgagtg tcatgcgatt agctccaaag aaattcaaac
     7261 gagagattgc agaaaaagaa gcaattttgt cggtattgaa tgcggtagac gataaatgtc
     7321 cagtaaaaat tagtttttca gatatagcaa caattatcga aggtatttat tatggtgaag
     7381 ttgatgaaga gatatcttct acaaaagatt tattagattt tttggataaa aacaaagata
     7441 cctttatagt actaactgat tatgaggagg ttacaacaaa tgaaaaatta tgatccgaat
     7501 agccgatggg gaacacatac gattaaggtt tctttccaac gttgggatta caaaggattt
     7561 gtgacgttta gaagaggcgg aaattgtaaa ggattagatg ttttagcatt agacgaggat
     7621 gatttatacg atcaaaaatt gactgataat ccaattggat tcggcttgct acctgaagat
     7681 gatcaaggta atgaatggtt taaaatgacg cttatgaatg ataatggaga cgaattatct
     7741 gttgaagata cttggtcata tttgagtgat tatattgtca gcgttgaaat cattgaattt
     7801 gtagcggata aagaagaata aaaactggag gaataaattt tacagataaa ggcttagaag
     7861 gagtcacgac gattaataaa aaggtagtat taccttaggc attgagtaag gaatttattt
     7921 aggaggaaaa ttagatggcg cgaccagttt tagaggcgat ggatatggat actttgacaa
     7981 aaggaaggta tgtacagctg ttagctcgtg gttattcacc taaagaagta ttcaaatccc
     8041 tgtctaaagg aacagatatg gaaaaagaaa gattacgaaa agaattcgat tattggagaa
     8101 cccataatgg aatcaaggat ttgaaaccag cacggccagt aattaaacga aagaaaaaga
     8161 taacccaaga ctgaagtgta gagcgtatgt gatcataggc ttttttattt ttgcaaaaaa
     8221 caaggaaggt aggaaaactc atgaattatg aagaaatcga aaacagaaaa aaagtgtcga
     8281 aagaaatgga agaaaagttg ctaaaaacga tgaaacaaaa acaccttaaa agattatctg
     8341 tcatgcaata tatcaatgat atgcagataa caggaaaaga gaaagcttgt ttgttaggtt
     8401 caatgaaaaa ctttgaacag ttgagacgaa cttatgtgaa aaaaagttct aattgtcaac
     8461 tattgttaga agtctcatga cattcattaa tagaattcat ttttaaaact ttttctatgg
     8521 aaatgagaaa aagatttttt gttttgtctg aaagggggta gctggatgag aaaatggtta
     8581 tttggttttt taggagtcgc tttaatcgtc ttttgcagtg tgtttggcta tgttagctac
     8641 caaaaacatg aaggaaaggt gtttaagcaa aatatagaaa aaaagatgcc cgtagatcaa
     8701 atcaatgaac atgcaaagag ttataaggag gatgcaacga acgtcaataa tgatatgtct
     8761 ttggggcaaa tgttgtccat acaaaaagaa gcaatcgaaa tgggtgtcaa taaacaagcc
     8821 tttgcacaaa ttcagatacc tgcacttggt ttagcattgc caatttttaa aggggcaaat
     8881 caatacactt tgagtcttgg agcggctact tatttttatg aagatgccga aatgggcaaa
     8941 ggaaactatg tcctagcagg tcataacatg gaaatgccag gtgttttatt ttctgatatt
     9001 caaaagttgt cattagggga ggtgatggac ttagttagta acgatggcgt ctatcgttac
     9061 aaggtaacgc gtaaatttat cgtacctgag tactttaaat taatcgatgg cgttcctgaa
     9121 gagaactctt tcttgtcttt accaaagaaa ggagagaaac ccttattaac gttgtttact
     9181 tgtgtttata cgtcacaagg aaaagaaaga tacgtggtac agggagagct acaataacaa
     9241 ggttttaatc aacgcgagtc gttgaaatat gaaatgagga gagtaaaaaa tggcaataaa
     9301 tcggtatgta aaaaaagctg gaatgcttgt tggaatgtta ggaatcattg caagctgctt
     9361 tcaggggtat caagtacacg cagaagatgt cacgcaaaaa acaccaccag agaaggtcaa
     9421 tatcacggtt cataaattaa tgtacgatca aggaacgcaa ttgaatgttg atattgacgg
     9481 gatcaaaaac gatggttata cgcacgatca atatcctaca ggtgtaacga aatacaacaa
     9541 ggcagattat ggtgatgttg agtttacgtt aggaaatatt acggatcaag tattaccgag
     9601 agaagattct gatctaacca acgcgaaagt ggacgagatt gtacaagatg tagaagacaa
     9661 gggatctaac agtgagtacg tcaagaatgc aaccaacaaa attacttcag ctgtagatga
     9721 aaatggtgag atcactttta ctgatcaacc agcctatgtc aattccaaag ggaatgtata
     9781 tgtggtttat gaaagtaaat ccgctgctgg attagtcaca caaaaagcaa aaccaatggt
     9841 tgtgattgcg ccaatgacag ataaaacagg atcaggattt ttaaaaaata ttcatttgta
     9901 tcctaagaac attgtttcta agttgagttt tgaattaacg aaatttggtg acgatggcgc
     9961 agcccaaagc aaacaaaccc ttttaaaagg ggcaaaattt gagttataca agggcgaacc
    10021 tggaaaagga acaaaattgg gtgatcttgt atcggatgat caaggaaagc tgactgcgac
    10081 tgatttgact ttaggaaaat actattttgt ggaagttcct tcggaagttg tagtgggatc
    10141 agatcaagaa ccaacagcag atcaatattt attaggcgca gacgctcgca acgatgcgca
    10201 taataaatta acctttgaaa ttaccaatga cggcgtgaca agtgacttaa aagcttccta
    10261 cgtgaactat aaagcaccag tgatcgataa aacagtgaca aatggaacag gacaagaaca
    10321 ttctttccaa attggtgacg cagtgaatta tcaaggaacg attcatatcc ctacagatat
    10381 tgcaggtgga acagatggga ttacagtaaa tggtgtgaag agtgaaactt ctccttacag
    10441 cgtatttaag tggggagata cagcaggaca aggactttct tatgtcgctg ctaaagcaaa
    10501 tatcaaagta acaaataaag atggatctgt ggtattaaaa gaaaatacag attataaaat
    10561 ccaaaattca gaaaatggct ttgtgatcga ttttatcgtg aataatggcc aagtaagtga
    10621 tactgtagca agtttacacg gacaagatct tcaaatgact tataacatgt atgtaaatga
    10681 cagcgcagct gttgccaatc cattaacaaa tagcgtggat ttcgtttata acaacaatcc
    10741 atttaatcag gaagaacacc acgaaaaaac gaaagctgac gttgtgactt atggtgcaaa
    10801 attcttaaaa gtcgatagcg gactatttgg aacaggaatc aaagcaacac cgcttgaagg
    10861 cgcagaattt gctgctaaaa acgcagaagg aaaatattat ggtggattag ttgatacgga
    10921 taaagacggt gtcaaagaag cagtatgggt agacgatgta gcaaatgcag cgatcctaaa
    10981 atcagataaa gaaggtcact ttgaaatcac tggtttaact gaaggtgaat attcactgga
    11041 agaaacaaaa gcaccaactg gttaccaaaa attaacagaa gatattgcat ttaaagtaaa
    11101 caaaaactca tttaaagagg aaaatcgaat cactgttaaa aataatcaaa aagcagctat
    11161 tccattaaca ggtagtaatg gtttccaaac gtatgtattg atctcttgct tgttgctagg
    11221 agcaggggca ctttctgcag ttgtttactt taagaagaaa gcctagaagg atcagctaat
    11281 gattattcgt aagcccccaa aaagaagagt catgcacaaa ctgatgatat ttttttggtt
    11341 gctgtcggca gtagcagtct atttaattcc agctgtggaa acaaatgtag cgaaatttca
    11401 acaacagcaa agaattttag ccattgaaaa tcttgaaaaa acagattctt ctttggatgt
    11461 tgcgaaaata gagttaggcg atcctgttgg tgttcttaca attccaagta ttcctttaaa
    11521 actaccaatt tatgacggaa cttccgacaa aattttagag aatggtgtag ggattacgga
    11581 aggaactggc gatattacag gaggaaacgg taaaaatcct ttaatcgcgg ggcacagtgg
    11641 actttataaa gataatcttt tcgatgattt gccttcagta aaaaaagggc aaaaattctt
    11701 tattaaggta aatgaagaac aacatgccta tcaaatttat cgaattgaag aagttaaagc
    11761 agaagaatta caaaaaaatt atttagatta tctcatgcct gaaaaagacc aagataggat
    11821 cacgttaatg acatgcacgc caaaaggtgt taactcacac cgctttttag tgtatggtga
    11881 acgagtgtca ttcagtaaag aagatttaaa actacagaaa aaagcaaaaa aataggaaat
    11941 caatttcatt ggattggcga ttagcactgc tattcgcttt ttttgcgttc ctattatttt
    12001 tatttataaa aaaataagga ggagatcgga atgaataaga gaaaatggct gtttgtttgt
    12061 gtagcagctc tctttgcaac tgcagcttac tcacagatat cttttgctga tagcagtact
    12121 actccaacta ccagtcagac aacagatagt tcaacaactg caacaagtga cacggacagt
    12181 tcgaagaaca ctgcagaaga aacggcagat tcaagtacga gttctactga atcagcggaa
    12241 tcaacgtcag aaagttcttc agagacttca gaaaaactgc aagaagtaga agatacgcaa
    12301 gtaatcaagc aaaacattca tattcaaaaa attatttctt cgaatcgaat caacaatcaa
    12361 ggaaggcaag tgactcagca agaaggtgtc aatggtgcaa agtttattgt ctacgatatc
    12421 acgaatatct tagaagaaat gacacaagat gataatgaga aaaatcaatc ggtagaaata
    12481 attgagtcaa aattgaaaga tcgagcaaaa aaactttcat acgatcagtt gaaaaaagtg
    12541 actgaaggag aaacccaaac aattgatgat caggaaggcg tgattgattt ttcaattgaa
    12601 gtacctgcaa acaaaaaaca agcatactat atcgtcaatg aatcaagccc tgaaaacatt
    12661 tcaaatagcg aagatattgt tttattgaca cctgtttcag atgaaaaggg tgaatttttg
    12721 acggatgttt ggttatatcc taaatcaaag aaatcagagc cgaaagaaga aattaaaaaa
    12781 gttgtatcaa ctggtgtaaa gaaaaacttt tttgataatt gttgggattt tttcactcat
    12841 ttatttttta acgattagga tgaaatgatg aaaataataa aaatgttagt ttttaatcta
    12901 tcttttttag ccgcaacaat attgtgttgc ggtttagttt tttctcaatt atcaatttac
    12961 ccaagatatc aacgattgtt cgttcatttt ctaaatatcc agcatgcttc catgccacta
    13021 gaagtgatta acaagctctt aatcaatcca atttttatgg ctagtttgtt tcttcttgca
    13081 ggattggtaa cgtgggtatt aggaaaaaaa gaatgagaag cttacaatcg tttaacaaaa
    13141 taatagatgg agggcattat ggaaaataaa attcattgca ctacgggaag agtatttact
    13201 ttagtgttgt ttttgttcct tcttcttttt gcaaggggaa ctgcagtcca ggccttagaa
    13261 aaagttgatc cgagtcaaat caaagaaaat aataatctgg tactttatca tgatagtgat
    13321 tcattgagaa atatcgaagt aactgggatc aatggattta agatactaga tgagtattca
    13381 actaaaaaac aaaaagtact tgcttggcca aaagtcaaag atttgaaaag cacgtcaaaa
    13441 attgttgccc attatcaaaa ggtgggcact tatctaggaa aagaaattga ttgcgacgta
    13501 actttttcta attttgtagc gcacgctggg gttaaccaag acggattagc taaaacaatt
    13561 aatgggaaaa agtattataa ttatttgctc tttgataaaa acatttatcg aggctttttc
    13621 atgcagtcaa tcaacgttat gaatgttaaa ctagatttta agtatgcagg atcaaaaaca
    13681 aaagtagctt tgagtgaacc acagccagaa attaattcaa aaatgagatc gatgagagca
    13741 gctggtattg agttgaatga tacatttcta tctatttaca gtttaaatgg acagtatcag
    13801 caacgtgtgt cttggtataa tgacaataat gaatctgtcg gtgaacgtaa tattgatatg
    13861 agagaatttg ttgggtacga aaaaatggat cgcttacctt attttgtcac aacagataca
    13921 gcatgtgcag aatacaaagc tcctgcgtta tcaacaaaca atttaacttc aaaagtagtg
    13981 ggaggacgtg cagaaaaggc ttatagctcg aatgatcatt tcaccgatgt tgtaggagct
    14041 gatacttatc acagaagtgg tgtaacgtat acgcttcaag gcgcttcccc aacattcatg
    14101 attggcgcaa atacgaatag tatgatgttt agctttgata ctgcattgct ttggacacca
    14161 caaccatcga agcctacaaa agaagtgttt aacaaagcta atactgaaga ggcagcacac
    14221 aatattgaca aaaaagtgat tccacaagga tcagatgttt actatcatat tcaccaaaag
    14281 tttgatgcat taacagtcaa cacaatgaac aaatacaaat catttaaaat cactgatacc
    14341 tttgacagca aaaattttga tatggtatcg gatgggaaaa actatgatgg cgcattgtgg
    14401 tatgttgaag cgaatgggac ttggaaaaaa gctgatacag gaggaaaatt aactgtatca
    14461 ggtaatacag tcacttatac tgcttctgac agctttttag ctaatgctgc aaattacggt
    14521 agggactaca ttttgcagct ccatatgaga gcaaaaaatg atgcaaaagg aattgcacca
    14581 aatgtggcta ctgtaacgtt taacaatgat ccgaacaaag gaacacaaaa aacaaataaa
    14641 gtagaaaact atttctctct taaaccaaat aaaaaagtgg aacaagatgg gaaagatgtt
    14701 gatggtcgaa atgatggtaa aaaaggaacg cctacagctc ctttaaatgc aggatctgaa
    14761 gtacaatatc tagtcacaca aaaatggcat acaaaaggtg tagacattgc gctcgatcat
    14821 tacaagcaat ttagtattca agatccagtg gaagctcgtt tgacgtataa agaaggttct
    14881 gcgcgagtga tagatatgag tacaggaaaa gatattactt cagaaggtat actaacctat
    14941 gatagccatt caagaacgct gaaatgggaa gcttccgctg atttcttaaa taacaatctt
    15001 ttagatggac gagaaattca actgatattt acagctaaaa ctcctttgaa tgaagaattg
    15061 gatatcgaca accaagcaaa agtttcagta gaaagtattg agaaagcaac taatgtagta
    15121 acgattgggg taaaacctaa cttacctcag gtcattgtgc caaaaactgg atcgaatcat
    15181 ttagtgctaa ctgttattgg aagtatatta tttcttactt tagcatcatt gggctatgta
    15241 gttttaaaac tcaaataaat ataaaaacaa ggctgatttt cagccttgtt ttcttttatt
    15301 tgaagattgg attcgcacta ataaatacat tatctacgcc aattctacca gcagagtcaa
    15361 ctacaaaatt atgacctaat ccgagaatac tttggttatt ataaagtaaa tttgcattat
    15421 gggcagggct atttttccat cctaccatgg catcgatagc accatcgtct gataaaaggt
    15481 catcaatata cagcccagtt tgacttatgt tttctccctt agcaccatag cagcctaagc
    15541 tttccaataa atcttggaaa gtaccaccac cagggcgata gtgactgaag ctgacttgta
    15601 attctttagc tcgaatatct gcagcctgtt gaacaatatc attgaattgc attggggcaa
    15661 caccattttg ttggcgataa tcatttaacc aagcaagttc tttttgacgc ataaagttac
    15721 gtaattcgtc taatgaaatc tttttatatt tgaatgtgat tgttttattt tgatcgacgt
    15781 tattaatgga tcgttggccg tttttatcca cggtaacggt aggatcgtca tttctgcaca
    15841 agaaataatt ttgatcagtc atatttttcc aaggagtaga gaagctatcc ccttcgtata
    15901 ctgtttttga ttcactagag agaacagcgt tggtatcagc atttacatga ttaacagtca
    15961 cattaaattt acgagtgtag ttgaaaatat aattttgact ttcagtcaca ttgtttaagg
    16021 ttactgaatt tgttggttct acccgatcgt ctaaaacata tccttgaata gaaggagcag
    16081 ttaaagtgac actttcaccg tgagtgacgt tgattatctg ttctttcaaa atatcaactc
    16141 gcccctcatt tttgtagttg ttattgcaca acaaattgat ttgatgcact tgattataat
    16201 tgaatgtgat tgttgtatta cctgtgattg ttttgctttg agtatcatta ccaatcactt
    16261 tgtagctaaa attatcttta gcaccttcgc tatatttaaa tgcttttgcc ttagcagtgt
    16321 aagtagaacc agaagtcact tttgcttgat ctgtagcaat cacttgacct gtttgattgt
    16381 caatatggtt aatggtaacc atatattctt ttggcgtttc aggtttgtgt tgcaaccctt
    16441 taaatgcagc atcaagacta tttttagcat tgtttacttg tgtttgagaa accttagaat
    16501 cgtctagtac ttttttagca ttggataaag cagtctgaaa attattccaa gattcttctg
    16561 taaaatctcc tttagcagtt gattttactc ggttgtataa agcttgtaat tcagctttat
    16621 tcacagtagg aactggtttt ttctttaatc ctttgtaagc agaatctaat ttatttttgg
    16681 cactatccac ttgcttttga gtcgcttttg agtcatttaa cactttctta gcattgttta
    16741 aagcggtttg gaatgcgttc caggtattgt cggtatagtc accttttttc gttcccttaa
    16801 cgctcgtata aagagcttct agagccgatt tattcacttt gggtttagag gtgtcagcgc
    16861 cataccaacc gataccttcg tatttccagc catagtcttt ggttaaccat tgtacttctt
    16921 tccaacttgc agtgtagtga tgtgagccag attttacacc aggaaggaaa aggcggtaga
    16981 tcggtttatt atttttttcg gcagaacgcc aggcaggccc ttcataagtc cagccgtaat
    17041 ttttagtcaa ccatttcact tcatcccaac tagctgtgta atggtgatca cgaagacctt
    17101 tattgtacaa acgatagaca agatggccac cgctagtcgg tgcttcccag gcggggcctt
    17161 cataatttcc ccattttatc tttactagat ggtcacgttc ttttttgcta gaggtgtaaa
    17221 aatgttcatt attatttgga ttgtaaaggc gatacatgac gttagttgaa gcagcgtcaa
    17281 cagatccagc aagactaagt gaggcagcaa atgttccaat agctaaaaca gtttttggat
    17341 taaataattt cattttttca actcctaaat ttaattaaaa atgtttacat gattattata
    17401 tatcggttaa ccgatatagt caataaaaaa taagaaaagc cttatataat tttggctttt
    17461 cttattttat ttgttagttg aagtattaat atcaacgatt tcttcagcca atttatttaa
    17521 tttcatagca tttatgaatc tcatgttgtc tatactagag cgtttgttgg caaggtcact
    17581 tactgtagtt aagggtacgc cagattctat ggatatagaa cgaactgatt taccactatc
    17641 aaacagcaac cattcgattt tctttttatc agcgtacatt tcaatgctcc ttcttgtttt
    17701 ttctttatta tagctctttt ttgtaatgaa tcacaatatc aagagaaaaa ttttctatag
    17761 aaaggtggat ttttatgagt attcgttctc cgtgtatcca aatgaggttt gttgctactt
    17821 tcgttatttg atatactaaa aataaacaac gaaagaagga ataaaatgaa gaaaaagcaa
    17881 ctatggtttg tctctatgtt tgcaatattt tttattattt taagtggatg ttcaagtaat
    17941 aagaaatcgg aagctaaaga agataatagt ttattgcaac caggaactgt ttggaaagaa
    18001 gaagtaggtg gtcttattca taatttaaaa attatagatg atactacctg ggaatattct
    18061 gaaagtgtat ggcatccaga tcctgtacaa ataacagtta agaggcaaaa agactatgaa
    18121 ggtttagaaa gatacaaaat agtagattct gcaggcatta gggaatttat aaacaaacct
    18181 gattcattat ttattgttgt tccctatgag aaggatggcg taaaaaaaat aatttttctt
    18241 ggatctagca aggatgagaa gcgaacgaaa gagaaattaa ttcatgatgg atcacaatca
    18301 aataaataca aattacaaaa gactagcgag tgattgctag tctttttatt tgtcctgaag
    18361 gaggaatcac agtggcaaaa gctaaaacgc catttgaaaa agccgcgtct aaaatcttag
    18421 aacgccgcgg tgtatctgaa acacagtgga aacaagaagt ccttgaaaaa gcacaatttg
    18481 acctattacg gggaaaagac aaggacttag aaggatatat ctatcaacgt gaacgtgaaa
    18541 agcttgtttt cgatgaaatt attaaacact aaaaggagcg aacaatttta tgtttgaatc
    18601 aattacaatg aattttttaa cacaaccgaa agtagtagct gcggttgata tttttagagc
    18661 attacaaact gccgcaacta gtttgaaaaa taatctgatg atttttgctg ctgttgcggc
    18721 agtggcttcc attgctcttg gaggtatttt cttcttcttt gggcgaagag gagctgaaaa
    18781 cgggaagaca cacgtaggtt atgtattgtt gggggtagga gttattttag ctgccgcttc
    18841 tattgtagtt tcaatgttct caatgtttgg acaaacccca caaggactat aaaaaaacaa
    18901 gtagaaggag taaaaagtac gtggctactt tttactcctt tttttatgga aggagaatat
    18961 tcatgttttt tacgatttca ggcaatgcaa aattggataa agagttaata agaaaagcaa
    19021 cgaagaaagt gggaaagcgt acttcctttt taccgaaaac aattaaatat tctgtcattg
    19081 aaacggaaga acagctacat gtgttaggac aagtgttaga acaagagaat caatcttctg
    19141 ttaaattgac tctcacgtta catgataaag aaggaaaaat agaaaaggct ggcacagaat
    19201 acgtttttgg ctcactggat tatcaaattt cagaaggttt ttatcctaca ttgattgatt
    19261 taatccgcct agatatattt aaagatacgg atgaaaccat gagtgtggaa gagtcttatc
    19321 gaaacgaaaa aattttacaa gttttagaag aagagttaga aagcgaccaa agaaaagtga
    19381 atcaagtgag tgaagtcgaa gcaaacgaag aagtaccatg ggaacgccca atctttccaa
    19441 aggatgaatc aaaagaaaaa gagaaaggaa ttattgacga acaagctgac attttgactc
    19501 ataaatcaat atttgaggaa gagaacgagg taatgggtca attggaggta gatccctttg
    19561 aagaaacaat ggaggatgaa gagacggccg aagtagaagt tgatccagtt gaagctaatg
    19621 aagacccaac atggactgaa ggggattctc aggaatatat tgaaccactt atcagtgaag
    19681 aagtagagaa aaatgaaaca gagatacaaa aggcaacggt atttgattat attttggatc
    19741 aatatccgcc agagcatgaa tggatgaaag aaaaaaataa attattttta aatgaacttt
    19801 attcgaaata tcaattacct caaacctatc aagcattttt agagagtaaa gaagaattaa
    19861 tctcacaggg taaaggcaca cttttagaac agttagagtc tgtccaatca acaaattggc
    19921 atgagcttgc acaagaagat ttattagcta cttttgaaga acgtcaacaa gaaacagatc
    19981 aagaaattaa tgagtatgcg acggaacagt cgtcgaattg ggagaaagca aaagaggaac
    20041 taaataaaga agaacagcga gtgattgaag aagaagtaca aaaaattaaa gagcggtacg
    20101 aaaagaaacg tgacaatgcg tattccttgt gtgtagaaaa gatcaacaat tttactcaga
    20161 cgaatcaaga agcattatcg aaagaaaaag aaaacttatt aaaagaacgt ataaaagaac
    20221 gaaaagaaga attttaccga gggttgcgta ctgataagct aaggattagc aaagaattaa
    20281 atgaacaact gaataaactc tatcaaacta cgactgaagt tctgatggaa aaacacgagc
    20341 agatccaaca agaattacaa gcacaaattc caaaatggaa aaaagagtat gaaattcacc
    20401 aacaagaatt gaaagaacaa gaagaaaaac aacggaaaaa agaaaaagag cgcgaacaat
    20461 tagaactaaa aaaacgtgaa caggatcttg cggaacaaaa aatggcgata gaaaaacaac
    20521 ggctagaaca agaaaaggaa aaagaagcag ctgctcgtaa ggaacgtcaa gaacaaatgg
    20581 atacattgcg acaagcacag ttaaattatc tgacacatcc tcaaccagta gctgctgttc
    20641 aaacgccagt gaacaaccaa gcaaatttaa ctccttcacc ccagcgttcc tttaacggat
    20701 ggatggtggg atgtattgca agtttaagct tgcttctagg tggaggaaca gtatttgctt
    20761 ttaatcatat caatcaatct tcacaggctg attctgtagc cacaacggcg tcaatcaagg
    20821 aagaagccca ggaacaagca agaaaagagt atgaacaaca attagaagaa ttattgaatc
    20881 gtaccaatca gtcaacaaca gagtcaagca tggaaaatag caaagagagt gattctagta
    20941 aggctaaaga aggatcaagc gattcaactg tacaatcaaa cacttccgaa agttcagaat
    21001 caaatgaggt gaaataatgc gtgttagaga aacaacgaag cgaaaagaca aacgattcaa
    21061 tttagaaaag aataggtatc tttctcattt ctatcgctat actccctcag gagaagagct
    21121 accagaaaaa catgacaaac gcatttcgaa aaagattttt tatagtggac ttgtatacct
    21181 ccctttagag tatttagtaa caagagtacc agaagttttc aaccgattgg aaattgttaa
    21241 gggaagctta caaggaataa atctgtttca attacaggaa atgccacagg tctttcattt
    21301 tgattcagct ggtttttgga atccatttac tcctacaact agtttgggta cacgtttagc
    21361 aatttcgaca gtttgtgtgg ttccagctgt acttatggga ttgaaaatgg acatgtggtt
    21421 tcgtcctcta gcagatggac aaaaagggga taatcgatta atgaccttaa aagaaatcca
    21481 acagatttat ccgcaaatac ctgaaacaaa aactactttt ccaggttatg gaggtattcc
    21541 aatcacacat tacaaaaaat attggtacat tcaaacagat actgttaata cgtgtattgt
    21601 tggtacctct cgatcaggga aaggtcagat tgaagtgtta gccaccattg acaatttatc
    21661 tcgtgcagaa aaacaaagtt caatggtcgt taatgatcca aaaacagaat tatatgttgg
    21721 tagtaaagat gaactagaag ctagagggta tgatgtatat gcctttaata tcaccgatcc
    21781 actgcaatca atgagtgata atccattggc actcatcgtg aaatactgga aacgaggaga
    21841 catagatact gcaacacagt taaccaatac tttcacaaat acgatttatt atgatgccaa
    21901 cgcttcagat aacaaatact ttaacgaaaa cgcgcagaaa gctgtcaatg cgcttatttt
    21961 tgcgttgtta gaacaagctg atcaaacagg agattactcc aaattgacac cgaataatct
    22021 cgttgagctt ctttctgaga ttggcggttt taattattat ccagatccag acaatgaatt
    22081 caaacagttg aatgccttag atgaatttat gaatcagttg ccacctggaa acattgcaaa
    22141 aaaacaatat ggggcaacga aaattggatc tgataaagcg aagggaaata ttttatcaac
    22201 ggcaatcacg ggattgaatc catttactct aacgaaaatt gcaaagatga cttctcaatc
    22261 aacgattgat tataaatcgg ttgggtttcc aaaatactta gatttgaaat tacatgaaag
    22321 tcttttaaat caacgagtcg tagtagaatt ttatcataag ggaaagaaat ttcatgaaga
    22381 acgtgtcaaa gtaggctata aaggatttag tgaaatcaat tttgattgtc atttagttgc
    22441 aggtgatcaa gtttgtttaa aatacgatca tcaaggaaaa gaatatgtgg cttggtatga
    22501 attttctcaa agagtattga aagacgaaga gggtaatatc gtttatcgga aaaaacgagg
    22561 agaaacccat ttgccagaat tagaaaaaga agccgttcta acattagatg aggaaaaaag
    22621 taacttatta gtagagtcta ttcggatgca ctataccgat aaaccaatgg ctatttttat
    22681 gcttacacct gattatgatc catctaacca tatcattgtt agtatttttc ttagtcagtt
    22741 atataaagaa ttatctacgc aatgtgttaa gactaaagga gataaatgtc accgcaggat
    22801 tcattttatt ttagatgaat ttgggaatag tgtgacctgt ttagcctaag gataaaagag
    22861 gctagttcca acgaacattg gaataacttt actatcgaga aatcaaatga cggggtaacg
    22921 ccctgaaggg tttccactga tactgcgaca tgcgttagta ggatgtgcat ccaaacgcgt
    22981 atgaagctcg ctgaagtcgg cagaagggta ccgtaaggaa agacacgata cgtatctacc
    23041 acgcaagtgc tctagaggta gggtatgtac ctgatatgtc gggtgtccta aaaagttcaa
    23101 taaggtgaga atgatttgat tatctgacaa acttgcgaat ggaatgtctg aaacgaataa
    23161 ctcatagaaa tgtgaggggg aagggggata cgtcctcttt ccattgactc gaggttgagt
    23221 aaaaatgtgt ttctatgaaa aaccttgcat ggttaaaggc tatggagtag tcaacaggca
    23281 aaagcaagca cctaagttga aatctatgga tagatagtaa ggaacagggg aagcatctag
    23341 cacggaggga gcataccgaa gatgtgtttc taaggaaaaa ccgaaatcgg ttataactta
    23401 ggtgaataga tgtgaaagta gagccacgac cagtgaaaat ttcgtaatga aattggagga
    23461 acaggctcaa gtcgttattt ataatataag aaacctattt aaaatcatgg attctcgtaa
    23521 gactaaaaag gtcacgaacc agaatggagc gtgataaaac catgactaac acaaaaggaa
    23581 ttagcttaaa gaacaaacaa ctcagacacg ccgaatacta cgacatgact gagactttcg
    23641 atgaattgca cgcaatgagt gttgcaaaat caaattttaa aaatctcatg aaattcatta
    23701 ttgatgacaa taatatcttt ctagcgtata gaaatatcaa aaagaataaa ggaagtgcaa
    23761 cggccggata cgatggtgtc actataaaag acattgaaaa attggcatgt ggtgactttt
    23821 taactattat taaaaggcga tttaaggatt acagaccaag aaaagttaaa agagtagaga
    23881 tcccgaaacc aaacggaaaa cttcgacctt taggaattcc ttcaatatgg gacaggatag
    23941 tccagcaatg tattctacaa attttagaac caatatgcga agcaaaattt aataagcata
    24001 gctatggatt cagaccaaat tgttctacag aaaacgccat agcagattgt cttgctagaa
    24061 tcaatcaaag taagatgaat tatgttgtag atattgatat aaaaggattc tttgacgaag
    24121 taaaccatac caaacttatg cgtcaaatat ggacaatggg gattcgagat aaacaactcc
    24181 ttgtaattat tcgtaagatg ttaaaggctc cgattgttat gcgaaatggc aatgttgttt
    24241 ttccaaccaa gggtactcca caaggaggga ttttaagtcc actgcttgcg aatatatgcc
    24301 tgaatgaatt tgattggtgg atagctaatc aatgggagga acgaactgtc aaagagctga
    24361 aacaccacta tcgaaaaaac ggagcatggg ataaaggaaa tatctattcg aaattaagaa
    24421 gccacacaag tttaaaagag atgtatctgg tacgctatgc agatgatttt aaaattttct
    24481 gccgaacagg agaagaagcc caaagggctt tttatgccac acaaaaatgg ctcagagaaa
    24541 gattaaagct cccaatttca atggagaaat ctaaagttac gaacctaaat aaagctgaaa
    24601 gtgaattctt gggtttttca ataagactcc acaaaaaggg aaaaaagaaa gtggctttca
    24661 cgtacgtttg tcgcaaagca gaagaaaaca ttaaaaataa actaaaacaa cagattaaaa
    24721 aaattcaata cgctggagca aaaaaaaata ggcaccgagc aataggtgac tacaacatga
    24781 tggtcatcgg tatccacaat tactatcgga tagcttcaca ttgtagtgtc aatttcactg
    24841 agatttatag aagtgttagg acagtaatag aagcaagact gaaagctaga ggaatgacga
    24901 aaaatggaat ctatggacaa gatcatgaag gataccgtcc gtatctaaag tctaagcaaa
    24961 tgagatatat tgaaggtttt ccgatacttc caattggcta tattaaaaaa agaactccac
    25021 tcaacaagcg gagaaatcta aataaatata ccaaggaagg gagaaagctc attcatcaag
    25081 agcaacaagc tatagaaagc tggaaaatca agtatcttgt aaaaggtttt agtaacaata
    25141 agaaatacac ggtggaattt tctgataaca aaatatctaa gtttatttcc caaaaaggga
    25201 aatgcgctat cacaggaaac gaacttttga tggaagaaat tgaatgccat cacattaacc
    25261 catatcattt gtccaaagac gatagttaca agaaccttgt cattatctct agagaaatcc
    25321 atagattaat ccatgcaact agtgaagaaa ctattctgaa actgcttcgg ttactgaaac
    25381 taaatcacta tcaaattaga aaactaaaca cgtatcgaaa aaaagcagct aattttccaa
    25441 tatcaatcaa aatgaattag ataggaaaca aattatattg taattaacga tggaacgccg
    25501 tgtgcggtga aagtcgcacg cacggtgtgg accgggggaa aagacggaga taatatcaaa
    25561 ctcttaccta tcggaattgc caccaatgga taatatggaa ggaatcatga cggtcacagc
    25621 tggccgaaac atgttgtggg atctgtttat tcaatcttat cagcaacttt atgcgaagta
    25681 tggagaacaa aacgcagcga ttatcaaaga aaattgtcag acacatattt atattatgtc
    25741 gacagatgat aataccatcg aagaattttc taaaaaagtg gggaataaga cggttgaaca
    25801 agagaacagt accatcaatt cattaggtat gaatactcat atcaatcgca atgttgattc
    25861 agatcgtatc ttaactcctg aaagaatttc tactttttta gaaggtgaaa cgattgtact
    25921 cgctccttta aaacgacaag atttgaaagg tcataaaata cggccgtatc ctatttttaa
    25981 tcatggggaa acgaatcttc cttatgctta tcaatatatt gaagatttca atccatcgtg
    26041 ggatatcaat gatatagaca tacattgtac acatgcggac ttggatctta aggctttaca
    26101 aattgattat gattatttcc taccagatga atatgctaga aatctctatc atggactgca
    26161 tgatcaatta gaagaaaaaa acgaagaaaa tatgttttat aaaatcgttc aatctcttga
    26221 gaatgagacg gctaaaaaat tgttaattca attttacgaa gaacaagcag cagatcgact
    26281 tcaagaatat gttgaaaagt tgatcaaaaa tgaagaaatt tctgaaatga ttgggaaacg
    26341 gttgttattg gaatctgaaa aggtggtgaa agaatgatca ttggaggaat tttttctggc
    26401 atagccgact ggtggtcagg cttattcgga gaaggggatg caaaccccaa aaccgtaacg
    26461 aaagtcttac aagattatgg taattatctg cagtatcaag atgtgttcag ttggatttgt
    26521 aatacttttt tatgggggtt gatcaaaggg ttatataatc tcaatacttt actagaaaaa
    26581 accatttatc aaagctttga tttaaaagac ttattgaacg ccgctggggt aaatgaacta
    26641 tttcaaagtt tgattagtaa ggtattggct cttttttgtg tcgtcacatt aatttattta
    26701 ggcttgaaat tttttatgag taaacaccca ccaaaagtca aaaatatctt ggttaattta
    26761 tttatggcca tgatcttgat ttttggtggt agttctttaa tcgatgaagg gttaaatatc
    26821 agtaaaagct tttacggaga cgtcactgca gcaaataaag cagataaaac gggttcacct
    26881 gcttttcaaa ttattcaaaa taatgtgatc gatgtcagta cattactagc aactgacccg
    26941 aataaagtag aatccctgcc aacagacaaa agaaatgctt taacagcgaa aaattttaaa
    27001 acagcgaata tcaatgggat tattactcct aaacaagcta aagaaatggc ggataaagat
    27061 aagagtggtt tatcagattt acaaaaggat cggttaagag cgttacaata ccgactggaa
    27121 ctggatgata atggaaaaga aattcctgtg caaatcagtg atgaaggctt agccaaatat
    27181 gtttatactt cagggtatcg tcgatacatt agtagtccag ggattatttt agctggtgaa
    27241 tcttctttgg cggtggccta tttatttatc ctatttacga tcgttacttg cattattgag
    27301 ctggtattta aaaaattcta tttagtgatt gccgcgtcaa cggactttga gacaggacaa
    27361 cggatgaaaa gtgctatcga agatatttct caatccttct tgttattggc atttacaatt
    27421 ttagaattac gaatctatac cttaatgctt tcaggtattg gtgatttgca tgcggcagga
    27481 aagatcaatg gattcttgta tgttgtcgct ttgattgtac taaccattgc gctattcaaa
    27541 ggctctcaat cagttacgaa aattttcggc gttgatacga gtctgaaaaa cggcagtaat
    27601 tctcttctta gcatgtttgc cttaggaaat attgcgaaaa atgtgggtag cggagctaaa
    27661 aaccttgcaa gtgctggtaa gagtggttta agccatttaa atgatattcg taaaaatgga
    27721 tttggccatc gaaaagatgt tacccctgat aacggggaag aacaatcaga cgataaagct
    27781 acaactgtac ctaatagtaa gaaaacagtt tccgaaatgt tcgggaaagc gaaaaatggt
    27841 gtttctaaag ccgcagaagg tcttggttac gtcaatgaac gtgggttaaa aggaacagct
    27901 gaagatctta cagaaaaagc caaagacacc ttagaagata aattagacgg cacgagagct
    27961 aaagaattgg ctgaagctgt gaagaatcca tccgaagcac tagaaaaaca aaaagaaaaa
    28021 gcttcagaag tcaaagaagg actaaaagat gcgtatggtg aaggacaagt caatgcagca
    28081 gtgaaagcta atacaacaac cgaaaaagaa aatcaagaag gtatagtgat tccttcagtc
    28141 aaagatcacc aagaagcgtt gcactctaat gaaaattcga ttgaagagaa aaattccata
    28201 gaagaaagaa attcagaaga aggaaaaaat ggacagttac caaaaactga tccagaacaa
    28261 tatcgctata aacctaacag tgaccttgaa aatgagaagc gaatttcaga tttatcaaaa
    28321 gtaaatcaag aagacaaagc agaaaatgag ggacagttac caaaaactga tccagaacaa
    28381 tatcggtata gatctgttaa ctccgaaaat gaaaaacgca tttcagattt accaaaacga
    28441 aatcaagaag tacaaacgga tgaggattcc atcgttctgc caaatgataa tggaatcgaa
    28501 gttggaactg aaaaatcaac gaaaattggt ttacctaagg cacaaaattt accaatgaaa
    28561 gatagtcgtt tgaatcaatt caaggataat gatcaaagtt ttgttcatca atcacctgca
    28621 ggtactgttc aaatgaaacc aagtcgtttg cctgaaaatg gaaatctgac aactgtttct
    28681 tacaatttat ctgaaagtgg tgagacaggg aatgcgtccg ttcaaaatgt tgtgacaaga
    28741 gaagctgcac caaaacctat tcaaaacgtt catacaggtt acaagccagc aaatgtggca
    28801 tttactgcac caacaaaaat aaatgtgaca agagaaacta aaaacaggaa caatgcattg
    28861 aaagaacaac tattgaggga tatcaaacag gatatcaaac cataaaaggc ctacaagtta
    28921 ggccttttct ttatatgaaa aggagaaatc aattatggat aaatatataa acatgaaaaa
    28981 ccctccaaga tttagtggag aatggaagtt tttcgctttt ttctttttag atctattaac
    29041 gcttggtggc tgcttttttt gggcgaaaat ggtcaataat cttgtccact taccactctt
    29101 ttttgcactc ttgaattatg gcttatggat tgcgatagcg ttagtgattg tttggcgacc
    29161 ctataataac ccaggtcaac gacaaatttt tgcgattatc gagtctattt tttttgcgga
    29221 caagaaccat tatcatgcgt tagatcctaa ccgaaattat catggtacgt ataaggagga
    29281 tgaataaatg cgagtcaaag gacaaaattc agttgaaagg aaatccattt tgcagtttgg
    29341 aaaaccagaa gagcgctcta tcttggactt atttccgtat aaatcctgta cagaagaggg
    29401 agtcttaatt actagagaag atcattttca acgttattat cgtgtagttt ctacagacgt
    29461 ggaaggatta aatgaacaag agaaactaga acgaatgaat cagttgacca ccgtcatgcg
    29521 tacgtatgtg ccaacaatta agttagttag tttgaccaca gagacggatc taacggaaca
    29581 aatcgtgcaa aagcggcagt tattagataa aaatcgtatg gaacagtcct taggaagaaa
    29641 tctacagcga ttaagaaaat atgaaaagaa attagtagag gaaatccagg aattgaaacg
    29701 agcagaacag gaacgccctg atttaagctt ctttttcgtc attgaaggaa agacaatcaa
    29761 ggatttgcaa accagaaacc gtcagttaat gcgatctagt ggcattttag ggttaaaacc
    29821 actctacaaa aaagaattga tcaagattct ttatcgaatg aataatatga atgatgagta
    29881 ggaatgggga agagagaatg aaatatccgt tgatatggtt agaaaaagat gttccgaaag
    29941 aagagaccac tagaaaattt cctcacactt taagcctggg taagttggga aaaggaaaat
    30001 cttttaacca agtaaagaat caaacaagaa gaggtgactg tacatgttag aagcaattaa
    30061 aacagctttt tcaccacaaa atgatcaaaa ggtacttgat ccattaaagc aactaggaaa
    30121 tcgggaaata gaaattttaa aatccaaagg gtatgattta gactttttaa aacaagtgat
    30181 gccacaaggt ggattaaatt tcgatgaaga gtatgtgacc tatggaaatg gttattcacg
    30241 atgtgtgaca gtttatcact acacaaaaaa tcctacgctt ttttggttgg ccaatctcat
    30301 gaacaatcct catacagtgg caacactaga tgttagcaca gatgaaaagc aaaaagtgat
    30361 tgatcaaatc aatcgtgcct tagatgaatt aaatgatcgg tcagataatg aacgaaaagc
    30421 gactgaccga aataaaagcg cggaagaata cctagaacta gcggaatatg cccgaaaatt
    30481 gacttctaat ggggaaattt ctaaacaagt caaagtgaga gtacatgttt acggaaacac
    30541 gattcaagaa gtagatgaac gagcacagga actgatcgaa aatctcaaag ggatggatta
    30601 tcgtgcagtg acctatctgc acaaaacgaa aagagactgg caatcgatgt tcaccatgtt
    30661 cgatgaacaa gctgaatggt ttggaaatat caaagggcaa tcactaccat cgctaaacat
    30721 tggcggaggt ttcccgttcc accaccaaac gctaaaagat ccaggtggaa cacaatatgg
    30781 ggaaaccatg acaggcggcc cgtttgtttg gaatgccacg tataaaaata gtgtgcggtt
    30841 gtctaccaat accttgcttc taggaatgat gggagctggt aaatcgaccg ttcttaaaat
    30901 gatcgcggaa gcccatttag ctgcaggaga ctttatttgg ggctttgaaa agggaaaaga
    30961 ctttattccc tttttaaagg aatataacgg cattatggtg cgtttagacg gatctgacgg
    31021 gatgatcaat cctttagaaa tatttgccac acgtacgtat gatgaagctt ccagtttata
    31081 tgacgatgga tcagtcaaag atttgaagat caatgaagca gcgtcctatc aaacgcactt
    31141 agacaaagtg gtctatcagg tacagttagt gagtcctcag ctaaaaggaa cgatgaaagc
    31201 agaatttaag acctatttaa atcgttttta tgaagagtat ggaacagtac cacgcggctt
    31261 tacttcctca aattcgcgtt caaatactga tacgcaagtc acaggtaaag atccagaagc
    31321 ttatccgacg ttcaaagaat ttttagactt tttaggacaa ctggaattac caggtgcttc
    31381 ccaagagaaa aagaatcgaa aagaagaaat ggaatccatc gtagaatcct tatgtgagac
    31441 gtatggcatg atttttgatg gtcatagtac gattcgacac cttgaccaac aacagttggt
    31501 cttttttgac attgactcta ttacaggctt agatcgaggg gtacaacaat gtcaaatgta
    31561 catggctatg acgttgattt ggaatcaggc attgattcaa ggaagaagac aacgacgttt
    31621 aattaaagaa aaacaaatgg aagaacaaga cttcaaatgc ttcttggcga tgattgatga
    31681 gtgccataat ggtgcgacct gttctgcagt gaaaagctgt gggttgcaag tagtctaaaa
    31741 actattgtaa taactcttat aacgagaagt caaatgagtg ggtaacgcca caaagggctt
    31801 ccactgatac tccgacatgc aacaagtagt ttatgcagac caacgttgtg tgaagctcgg
    31861 tgaagtcggc tgaaggatac cgtaaggtaa aagaaatagt gaataccacg aaagttctct
    31921 agaggtaaag ggtgtatccg ataagtcggg tgtccaaaaa aacttaatat ggtgagtatg
    31981 tacaaacgta ctgacgaact agcgaatgta ctgtccgaaa ggcatagctc ctagaaatgg
    32041 gagagacagt gagcaaaaaa tcactcattg tgctaacttg tggttgagta aaagtcgttg
    32101 ttatgaaaga ccatgctgcg ttacaggcgt aggagaagtt agggggcaaa agctagcatc
    32161 taagttaagg catgtggata gatataaggg aacaggggaa gccatcaaca tggagagtgc
    32221 atactcgatg aagtggtagt aagaaatatg tgaatgacaa ataacttact ttagtgatgg
    32281 tgaaagtaga gtcacgactg atgaaggttc tgtaatggaa ctggaggaac aggctcgatt
    32341 cgtctgtaaa agtcatgaaa cccgttcaac catgggattc tcgtatgact aaaaaggtca
    32401 cgagccaatt ggagcgtgat aaaccatgac aaaagaaaca agtccaaaag ataggaagtt
    32461 gcggcatgca gaatattatg gcatgacgga aatcttcgat aagatatact ccaaaagttt
    32521 gaagggagat aatttcaaga atctaatgca gattatcatt tctgataaca acattgtcct
    32581 agcatatcgt aatatcaaga gaaatcacgg tagtgtgaca agaggagaag atggagtaac
    32641 gattaaagat attgaaaatc tcaaccgtaa tgaattcgta gaaattgtta ggaagcgatt
    32701 cgcacattat aaccctcgga aagtaaggcg tgtagaaatt cctaaatata atggtaaatt
    32761 aagaccgttg ggtattccat caatatggga tagaatcgcg cagcaatgca tcttacaagt
    32821 attagaacca atatgtgaag caaaattcaa taatcacagc tatggtttcc gcccaaatag
    32881 atcagcagaa cacgcaatag cagattgtat ggcccgaatg aatcaaagtc acatggaata
    32941 tgtggtagat gtagatatca aaggtttctt cgacgaagtc aatcatacta aactcatgag
    33001 acaaatctgg tctatgggta tacgagacaa acagcttctc ttaatcatta ggaaaatgtt
    33061 aagagcaccc gtagtacttc caaatgggaa gttgatatac ccaacaaaag gaacaccaca
    33121 aggcggaata cttagcccat tacttgcgaa catcaatctc aacgaatttg actggtggat
    33181 agcaaatcag tgggaggaaa aagatgccaa agagctaaaa catcatttta ggaaaaatgg
    33241 agcttgggat aaaggtaata tctatcacaa gctcagaaag agtacaactc taaaagaaat
    33301 atacctagtg agatacgcag atgattttaa aattttctgt agaaattata atgaagcgaa
    33361 aaaagttttc tatgcaacta aaatgtggtt acaggagcgt ctaaagttac caatatctga
    33421 agaaaagtca aaaatcacaa atctttcaaa agaagaaagt gaatttttag gctttacgat
    33481 gaaattagtg accaaaggta acaaaaaggt tgttaatacg catataggta caaaagcaca
    33541 aagcatgatt aaagtgaaac taaaaaaaca aatcaaacga attcaaaaaa gtcctaatag
    33601 caaagatacc tttatctata ttggagtcta taacagtatg gttattggga ttcataatta
    33661 cttcaaaatt gcgactcatt gtagtgtagc aatgcgaaaa attgcaagag aactcagacc
    33721 aagttttgaa atacgactac gagaacaagg aatctcaaaa gaaggacaat acggaaagta
    33781 cgaaggatac aagccttacc tacggtcagg acaaatacga tatataagag ggtttccaat
    33841 attaccagta gggtatattc aacacagaca tccgatgtgt aaaaaaagaa atattaataa
    33901 atacaccgaa gaaggtaggg aagccattca taagaagcaa caaaccgtag aagaatggaa
    33961 gatatcgtgg ttgtgtagca atccagtgct tagtaagaga gcaacgattg aatttgcgga
    34021 caatcgaatt tcaaaattca tcgctcaaaa gggaagatgt gcagtaacag gtgaggagct
    34081 tatcttatct gaaatgcatt gtcatcatat tatcccttat catgaaagca aaagtgatag
    34141 ctatgaaaac ctagtgattg ttactgagga ggttcatcgg gtaatacacg ctacacaaag
    34201 tgaaacgatt gaggaacttc taaaatatct aaaattaaat ccaaaacaaa aagaaaaact
    34261 caatgagttg agattaaaag ttggtaacga agaaatatct taataaatat ttgtaggacg
    34321 ggaaaacaga atgtctttta tagacgaggg aacgccgtat gcggtgaaag tcgcacgtac
    34381 ggtgtggact gggggaaaag acggagataa tatcaaactc ttacctatca gtatcgatta
    34441 atccagattt tattgaaacg gtaaattatg tcacaaactt tcaacgagaa atgcgtaaag
    34501 tagaagcaat gactgtttta gctacacaat ccccacaaga aatggtgcca gaaaatatgt
    34561 cttctgataa cttctcgaaa ctgaagaaag tctttgaatt ttcttctacg aagatcttta
    34621 tgcgtatgga tgaatctatt ttacgtcata tagaaggatt agttggtaaa tcaatgacaa
    34681 actcagaatt agaatccatt cctcaacaaa atcaaggaga cgctgtgatc aattttggct
    34741 caaaagaaac cattcgtgtt cattttagtc cgaacaaacg tcaattaaaa ttgtttgatg
    34801 gaggaaaata atggaaaacc aaaatgaaag tttaatcaag cagtatgtta agcgtcgtgc
    34861 aaagcgacgc ttatttttgt ggttgtttgg tacttctgca ggtttgatta ctattttgat
    34921 tactgtgttc gtgacccttt ttctaatact tgcggcgggt tcgattgata attctgattc
    34981 agattcaagt agtggtggcg aagcttttac aggagaatat tcggaaggct taccgattta
    35041 taaagaaatc aaaggaagag gccctttttc tgatgagatt gcgcaatatg ctgtaggtgc
    35101 agcagtgaaa tataaattat taccaagtgt gatcctttcg caatacggtt atgaatcagc
    35161 gtttggtacg tcggcgtccg caagaaatga tctcaactat tttgggatta cttggtttga
    35221 tggttgtctg tttcctaaag gaactgcgcg aggaatcggt ggtattgaag gtggctggta
    35281 catgaagttt cccaactcaa aagcagcctt ctcctattac ggctttatgg tagccacaca
    35341 atccaatttt aatgcatgtg taggaaataa aagccctggc gcaagcttat tgatcttagg
    35401 acgaggtggt tatgccgctg caggaattac tgaagatagt ccgtattata caggttgtat
    35461 gtccattatc acttctaata aattaaccga atatgatgaa ttcgccatca aacattgggg
    35521 tgaaggtggc aataacaacg gcacgatcac tggtgaatgg acgaatccat tccctggcag
    35581 cagtttagat aaaagttcct tcagtggtgg tcaattattt ggaacaaatc caggcggtga
    35641 attccgtccg aatggcttcc atgatggatt agactttggt tcagtcgatc atccaggaag
    35701 tgaaattcac gcggtgcatg gaggaaaagt cgtgtacgtt ggcaatcctg gtatttcagg
    35761 tttaggtgca tgtgtgatcg tgattaatta cgacggtttg aatatggtat atcaagaatt
    35821 tgcgaatagt acaggaaatt ctcgtgtgaa ggttggcgat caagtgaaag ttggtcaagt
    35881 aatcgggata cgagatacag cacacttgca tttaggtttc actcgaatgg attggcgtca
    35941 agcacaagga cacgcattca cagacgatgg tacatggatt gatccattac catctttgaa
    36001 tagcagcaaa aaatagatca ggagaaaaaa tatggaaaat aaaactttaa aaatcgtctt
    36061 aacagctgca attagcgtta gtttaatcgg caatatcgcg ttgttttggc agctgaaaga
    36121 agaacaagag acaaatacct ttaaaattga agaagtggct aaaaacgcgt ctgagaagct
    36181 caaaaaaaat tcctctgagg cacaggcgaa gatcgacaag ttaaatcaaa ccatcaatga
    36241 gaaaaaccaa gaaatcgatc agctgaagca acaagatggc acaaaagcaa aagaggaagt
    36301 aactgaagca caaagaaaga cggcagaaga gttcggtcaa ttggccattg ataaatcatt
    36361 aagtagaaat gaattaacgg aaaaactaaa agatgtcgca acccaagaag tgatcgacaa
    36421 attaagtcct gcagacgatg atcaccaaca tgatgattac ggaagttact cctttacatt
    36481 aggtgacgtg caaacttatg ctcaaacaag cagtgtaaaa gaggaacaaa attttgtcgt
    36541 atttgttgat tatacaatcg gcaatccgca attcaaagat gtcaaaccac aaaaaataaa
    36601 aggtggatta actgtcactg aaaaacaagt agatggtcaa tggaaagtca tagactttag
    36661 ttatttcact cgttaggagg aagaaaatga gtaagaaaaa aggagaaaga caacgtttca
    36721 aaaaacgaaa aagttatctt caatggctgg gaatctttat gattctcggc tttttgattc
    36781 ttgctagtcc gatttatacg aatcagaagg tagatatacc agatgaggta agcacgaacg
    36841 cctttttaaa cacgaaagag ctgacaatgg tttcaaaaga aaaaggagcg aaccagcaat
    36901 tagtttttga atttgcgatc gatgatgaag atcaaagctt gttaaaagaa ctggctaatc
    36961 ttaattatcg cgtagaagta aagacagcta aaggggatta tcaagcaata cgagccaaag
    37021 tgatcaaggt gtctgatgat tattttgcag tgaagctaac gcatgtacca gaagaatata
    37081 tcgccatgcg tttaaccatt attcctgaaa aaattgatcc aaaagtggat atgcaagaac
    37141 cacaagatct gatttattac gtgcacgaag ataaggttaa agatcaagtg aaagatgaag
    37201 attatgaaca acacgcgatc aattacaaag tgaaaggata caagaaagaa caaaaagcag
    37261 ctcaaaaaca aatggaatct ttccaagcaa ccattgattt gaatgaagaa ttagtgaaga
    37321 agctaaaaga tcaattgcct tatcaagtcg cagatgatca ggaaaatact gaaaacaaga
    37381 ttaatagcta ccagcaagaa attgaaacat cgaaaacgca actgaaagat caagaagaga
    37441 tcattcaaaa acttcaagaa aaaattgatc gtatgacaaa agaaaagatt tagaaggaca
    37501 ggtggagtga aaaaatgaag cgaacaaact tggctgacaa agatactttc gctgagcaga
    37561 aagtattgat taaagcctat ctcaaagttg taaaagctaa agaaatatgt cgagaagtga
    37621 atttatctaa cacaacaata tctgagttga agaatggtcg aaagaattta gatactttaa
    37681 aattcagatc atttgaagct ctatatgtag ctgctaaaaa atattttaca gctgataaca
    37741 aataaaaaac aggagtattt tatgtgaaaa aagtatataa aaaatgctta tgaatcagct
    37801 atatcacctt aaccatttgt taatgagatg ggagaaaaca ttaaccacga ttctaacgca
    37861 aacatcaagc acataaaaca tttaacactc ctcctttctg gctgaagatt tcaaccaaac
    37921 aggaagacag cgtatcaatt atagcataca ctttttctta aattcatttt tcatatgaca
    37981 aaatatttcc ttctaatcga caaagttaga aggatttttt attgtctaaa tacacaaata
    38041 accttaattt ttaaagagca taaggaaacg tgatatcact ttctcacgaa aggtgttagg
    38101 taggctgtgc ctaccacgtt tctaagaaag ataaatgata tcaaaatttt ggatttgtgt
    38161 gtagatttgt gggtaggatg aaagcgaatg tgggcagtaa aataagcgtt tgtgtgtagc
    38221 aaaatcaaaa aatgtgtagg gatatgtgta gctacacata tccctacaca taataaattt
    38281 ggaggaaaat atggacttat taattagaaa tattgaagaa aagtatatta ataaaattga
    38341 caaacgttgt gaagaactta cggtcaagac tggcaaaaaa tggagtcgaa atcaatacct
    38401 aaaagttttg atcgaaaatg attttgatca cgctcttctg aattataaaa aagaccaatt
    38461 tgatcggttg ttagaaaaat ttgtggatat tcaaacttat aatacgaaaa ctttagaaga
    38521 atatattgca acaaataatc aattaattgg tttgttaatt gaataataaa aatagtattg
    38581 tgaatttata aaaggaggaa atcaattttg gcaaaagcaa caagaaaaca tgtatatctt
    38641 tacgatatgc atatcgaagt acttgatgaa ataatttcta ctcatgaaga tatatctaat
    38701 tatagtgaag cagtcagaca tttgtgtatg atgtatgatc caaaaatcga agaaaaaata
    38761 aatccaattc aaattaaatt gaatgcaatg ggaaaagaaa tttctatttt aaccgaaatg
    38821 gtttcagagt ttgcggatct tcatttgaaa gatacaggaa tactaattgg tacaaattca
    38881 actgtttatc aagaagcaga agaacgtgtg gaaggaaaaa ttaaatccaa tcaaaccaaa
    38941 aaatattctt ctaagttaaa gaaaccagtg aatgaatctg ttcataccag agtagatcca
    39001 tttgatccaa cgaagattct aaaaagataa aagtagaagg agaacgtgat gaataaatcg
    39061 cctgcagtta ttcttacttg tcaatttacc ttgcccaata aaaaatcttt ttcaacatac
    39121 attgattata tgacaagaga aaaagcactg gaagaaatgg aacgacgtac gcccgaagaa
    39181 gaacaggaac tgcaacgaat caagaatgct ttagaggatt tttatattcc tgaaggagaa
    39241 actttttcgg aaagtaagac ggataaagaa ttcagtgaaa cgtcgaatga agctcaatcg
    39301 ttgatggaac atggaatcaa ttttgacaag ttagaggaac aagattttac caaatatctt
    39361 tcttatatga cccgtcacta tgcgttagaa caaaagaaag aactgacgca aagggaacaa
    39421 aaagaatacc aacggttgaa ccaagcaatg aaaaaatatc aaacagagcc acataaaaaa
    39481 gcttcaggaa atcttcctgg agttttttct atggcttcag acgaagtgaa aggaagtgat
    39541 ctgaaggaga tcaaacaaat cttccaaaga ggacaacaaa aaggctcaat catttatcaa
    39601 gatgttatct ctcacgacaa ttcgtatctt gaaaaaatgg gcttgtacga tcccaaaaca
    39661 gataccttag atgaagaagg tttgaaagca gctggaagaa aaatgatgga gacattattc
    39721 gagaaagaac aattgaatga aacaggttac tggatggcaa cgattcaccg taacactaag
    39781 catatccata ttcattttgc agttgttgaa aaagaaaata cgcgtaaacc gtatactgag
    39841 attgaaaatg gtgtgagtta tatcgtaccg aaggggaaga gaagacaagc aacattagac
    39901 gcaatgaaaa ccaattatgt acatgagtta gaacgtttct cttatgaaaa ggatcgaatg
    39961 atcggtatgg agaaacgaaa tttattgaca cgaaaaagtg atttgcgtaa caccttaaca
    40021 aaagtagtaa aagaaccagc acgttacgat cgacacgcca tgaacttatt aaatgaagta
    40081 tataaaaccc tacctgaaaa acgttcggaa tggaattatg gagatgaaaa tcgaaccaag
    40141 ctttcaccat ttacaagggg aaaacttgat gagttaacca aatatgtttt aacaaaagaa
    40201 cctggatatc aagaatatat acagatttca aaagcattga aagaagaagc caaaagcctt
    40261 tatggggaaa gcaaaagaga aacaaagaat gcagaaaaaa acgccctctt tgatttaaaa
    40321 aagcgcacag gaaatgcgat tttaacagaa ctgaagagac aggatagtca aataaaacca
    40381 ttaatagaaa gtgtcaatca gatgcaatct atttacaatg aacgatcctt tcgaacactc
    40441 aaacagttgc caacaaatga atacaagtat agttatcaag aattcgtaaa gcgtaggaaa
    40501 caattccaac aaagagttgt cacgcagcga agcttgcgtc aattaaatcg aacaattaat
    40561 gcacatgaag aaaaatacca cgcaagtaaa gcatatgaag agcaacagcg aagaatccaa
    40621 atggaacaag aaagacaata ggaggaagag aaatgaaaca atatttagat caatggaaag
    40681 tcatagaagg atcactaaaa gaagaacgaa tcgaacaact tcctgattgt ttagaaaaag
    40741 agcatttgtt tcagataagc gaaatgttga gaaacgaaca gtttgatcca aatcaatttt
    40801 tagttgttga gtacccagct actggtgtgt attgttgtaa tcatgtgaag ggtgaaaaat
    40861 attttattat tcaagaatac gaaggtaagt tagcgcctta ttatactact tgggaaataa
    40921 atgaagaggg aatcaataat ttcccatgtg aaagcattga agagtctatt tctctcacgg
    40981 aatgttaaat aaatagagac gtaaaagagt cctgaacgtg attgtccagg actctttttc
    41041 aatgaattgt tttccgccaa ttcactgagt ggtgcgattc aattttatta tatcaaaaga
    41101 aagtgagatt ccaaagtgaa gggaggaaga agaaatgact cagacaacga ttggtgttgt
    41161 ggttgaaaaa agcagggata atctcgtttt tgtgacagaa attcaaacag gtagagcttt
    41221 tgtagtcaca gacaaagctg caaaagcgta tcaaaatggc aatattttaa cgttaaatct
    41281 gactacgaag atgttcgttg atgcagtaga aaattatcca tttgtttaga caaacgatat
    41341 tttacaagga ggaaacggac atggcagaag aaaaagaacc actagataat agtcgtttgg
    41401 gaaagtccaa aagaaaatta gtacggttgc aaaacgaatt aaataatcaa tttcaaaagc
    41461 tatttgatca ccaaaaattg acaaatgggc aaccaatgaa tgacaaacga aatggtcgtt
    41521 catggtttaa ccagcaagaa aaaatagaaa ataaaataca aagtttgaga gaagagatca
    41581 agcaacaaga aaaacaggta gaaaaacttg aaagacaaga agagctaaag gaaatgggtt
    41641 ataacaagta cggtggcctg gacatgacga tcgaaaatat tccaagaatc aaagaagaaa
    41701 tcgaacgctt tgaaaaaggt gaatcgactt tttcagctgc cacgatccga aaataccaac
    41761 gaaaattgga aacattagaa cagctgaaag aaaaatcaga gaaaggaaaa gaaaatcttc
    41821 ttcctgaagt acaagcgatc attgacagcg gacgagtgac gcaatggaaa aagaatccaa
    41881 ctatctattt tctgaaaggc tatcgaaaag tcgctttaga actcaatgaa aacgggggat
    41941 ttgaggaatc taaaaaatac caagccacgt cggaagaaga aaaggcagct gttcgtgaat
    42001 tacttgaaga agctacacct actgattctg tgaaggagaa agaaaccgat catttctatc
    42061 gagtagaatt caacgaaaat gatccagcaa aagggttgaa aagttatggt ggggagatcg
    42121 taacactaga attgatcgat caactcaaaa cactggataa ccaatacgcc gcagaagcag
    42181 gctattataa attctatttt gaagaagtga agaatggaga agtagtcgat cagatacgaa
    42241 tggatctagg ggatggcgtc aatagtaatc accccatcta tttagagtta gctagattgt
    42301 gcgaacaatc acctgaaaag gaacaagaac aaacagtcaa aaatgaacct atttcaaaag
    42361 tacaaaacga gattaaagag acaaaaacaa aacaaaaggg ttatcaaaaa cgaacggcag
    42421 aagagattaa gcaagaagtc aaatcacttt cccaacaggc gcttgatgcg gtgaaaaagc
    42481 atactcattc acctgaagac gtcaaagaac ttttgaattt tatgtctcgt tttccagaac
    42541 gatcctttag aaatcaatta ttgatcgagg aacaatttcc aggcgcaacg gcttgtttag
    42601 gtcgtgccgc gttgaaaaaa gaaggaatct atatcaaaaa aggagaacaa gggaacaaga
    42661 tctttgtcag aaaaacggtc aaaggatttt atgagaaagg acgtggcttt gttcgagaaa
    42721 cagaggctac accagaagat aaaaaacgaa tcgaaagtgg tcaaatcgaa gtgatcaaaa
    42781 aaccttacta cacgattgaa aaagtgtttg atatcacaca aacacaattg aaacctgaag
    42841 actatccgaa aatcttcccg aaccgcgtct ttgattttca attggatcaa acagggcaga
    42901 cagaacttca agttgggatt caagcggtag ccaaaaatat tggaattcaa attcgagata
    42961 tgacagaaag tgaagtctat caaagagaat taggacaagc acgtggggcg tacgttcgta
    43021 acgaatttac tggcgaagaa gaaatcgtca tgaatactcg aaatactcct acgcagcatt
    43081 tagccacaag tattcacgaa ttagcccatg ctagaatgca caaattttca gaattagata
    43141 ctgcaacaaa agaactacag gcagaaatga cctcctatat tgtgtgcaag cattttggga
    43201 tggacacctc ggagaaagct attccttata ttgcgacatg gacgaagaat agtcagacgt
    43261 tggatgataa ggaagctgcg gaacgtggga aaatcatgaa tgatgtcagt cgtgtcgcaa
    43321 atgaattcat tcaaaccatt tctaatgaaa tcaatcaata cagagaaaga gaaccagaga
    43381 tccagccagc agaagtgaag caggagcata aaaatactgt agaaacggca gaacaaaaga
    43441 atattgtgcc cgttggctca ctttggatcg acaagaaaga cgggaaagtc ctggaagaag
    43501 acacgggccg aacgttacat ttgaaggatg cggcgtttat tgaagatccg cagcataatc
    43561 aagtgatttt aaaaggaaaa agttatggaa aagagatcca aacggcgtta ccttcagaag
    43621 aatttgcgct aggaaatcca ttccgatttt cacttgagac agtgtcagaa gtggcaaaac
    43681 gaaaaaatga aatctattat gaacaacaat tgtcacaaaa agaacgcgta taaaacaagc
    43741 aagaaagact atctgaaaaa tggatagtct tttttctttt gggaggtttg aaaatgacag
    43801 agaaaaaaat gagtctcatt gatcgatgca agcaaatgga tattgtggat tttgcacgaa
    43861 acaatggttt agcggtggcc aataaaggac gagactatcg cttagaagac catgattcat
    43921 ttgtttttga tcgaagaaaa caacgttttt attggaacag tcaaaatatt agtggcgaca
    43981 ttattgaatt agctaaactt tttttcatag acaaagagat ccaagaccct aagcaacaat
    44041 ttaaagcagc cactgatttt attctaaaga atgaagacaa aacagaacga gtggaaaatc
    44101 ttcattttga gacagaaaag tacaaagatc accccgttga ttatcaacca ctcacagaaa
    44161 aaggaaggaa ttatctgaag gaagaacgaa aactgcccga ttggttgatt gattatgcag
    44221 aaaaagaagg attgattgcc gaactaaagc caaaacatga acggcaaaat ttcttagttc
    44281 gagacgatcg tttggatcat gcggtcgcct ttttatggaa agatccacag acaaaagaga
    44341 cagtaggggc gagttatcaa ggaacgttca ttgattatga acgttttggt gagagaggca
    44401 catataagca tatagataaa aattcgacag cgaatcatgg ctttaaccta aagattggtg
    44461 atcctaaaca gttaaaattt tttgagagta gtattgattt acttagctac gctgcactga
    44521 atcgtgatca gttaaacgat acgtggttag tctctatgga agggttaaaa caccatgtga
    44581 tcagtcatta ttttggtgag gcagtatctg aattgagaaa gaaacaagcc tttcctcaat
    44641 ccatcgagat ttgtgtggat aatgaccgtg caggacatat tttctacgaa aaagaacaat
    44701 taatgggcgc agttgatcca tttacgaatc agaaagtacg ttgtgaacga ggaatcgcta
    44761 atgattggca agtgccaaga gaatacaaag tcatttatga agaagtcgca aaagaagaaa
    44821 aggtcacacc agaagctatt atggccatac acaagacaga aaacaatctg caattaacga
    44881 accaactggt ttccgctcac aaagtcaatg cgtcttttgg tcagcagctt tctgttaacg
    44941 attcaataga agtcattagt ttgaaagata tttgtagaga agtcgcaaaa gaattaaaag
    45001 attgtgaacg ctcgaatggt acgtatgatt ttgatcgttt ttatcaagga aaaggggata
    45061 ttaattctca gattcttttc tcttataaag cagaacaata ctacaaaggc tataaaaatc
    45121 atgaacatga atttgtacct gaagtgaaaa aggattggaa cgatcaattg aagcatgaga
    45181 tccatcaaca ggaaattaga aagcaaaaac gtgcgatgtt gtttcaacaa ggcagacaac
    45241 aagaaaggga gtgagatcgt cacgatgaaa actgtcattt tagcagaaaa accgtcacaa
    45301 gcgaaggcct atgccgatag cttttctaaa gccacccgaa aagacgggta ttttgaaatc
    45361 caagatcgat tgttttcggg tgaaactgtg atcacgtatg gttttggtca tttagtcgag
    45421 ttagattcgc ccgatatgta cgatgaaaat tggaaacaat ggtcattaga gcatttgcct
    45481 attttcccta cacattatca ctatcatgtt cctaaagata agaaaaagca attcaacgtg
    45541 gtgaaacagc aacttcaatc ggccgacacc attattattg cgacagatag cgatcgtgaa
    45601 ggggaactga tcgcttggac gatcattcaa caagcaggcg ctgatcaagg aaaaaccttt
    45661 aaaagacttt ggatcaactc cttagaaaaa gaagccattt atcagggatt ccagcaatta
    45721 cgagacgcag aagagaccta tcccaaattt gaagaagcac aagcacgcca gatcgctgat
    45781 tggttgatcg gcatgaacgg cagcccactg tatagtttac tcttgcagca aaagggcata
    45841 ccaggaagtt tttccttagg cagagtccaa accccaacgc tttacatgat ttatcagctg
    45901 caggaaaaga ttagaaactt ccaaaaagag ccttattttg aagggaaagc ccaagtaacc
    45961 gctcaaaatg gcatatttga tgcgaagctt gatcccaatg aaacccaagc gacgcaagaa
    46021 gcatttgaag cctatttaaa agaaaaaggt gttcaagtag gaaagcagcc aggcacgatt
    46081 cttgaagtcg aaaccgagaa aaaaagcgcg gcaagtccac gtcttttttc tctatccagc
    46141 cttcagtcta aaatgaatca gctcatgaaa gctagtgcca aagatacttt ggaagccatg
    46201 caagggttgt atgaagggaa atatctaagc tatccacgaa cagacacccc ttatattaca
    46261 gaaggagagc atgcgtatct gttggatcat ttagatgaat acaagcattt tttaaaggct
    46321 gaatcattcc ctactcctat tcatacaccg aatagtcggt atgtgaataa taagaaagtc
    46381 caggaacatt acgcgatcat tcctacaaaa acagttatga cagaagcaac gtatgaaaag
    46441 ctgacacctt tacaacaagc gatttatgat caagttttaa agacgacggt ggccatgttt
    46501 gcggaaaaat atacctatga agaaactacg attttgacac aagtccagca gcttcagcta
    46561 aaggcagtcg gaaaagtacc agtagatcta ggttggaaaa agctatttgg caaagagagc
    46621 gacagaaaag agaaggaaga agaaccgcta cttcctaaag taacaaaagg agaaacggtg
    46681 acggtagatc tacaaatctt tgaaaaagaa accaagccac cacaacctta cacggaaggt
    46741 acactaatca ccgccatgaa gacagctggt aaaaccgtgg atagtgaaga agcacaatcc
    46801 atattgaaag aagtggaggg aattggtaca gaagcgacac gagcaaatat tattgaaacc
    46861 ttgaagcaaa aggaatatat caaggtagag aaaaataaac ttgtggtgac gaataaagga
    46921 attttgcttt gtcaggctgt tgaaaaagaa ccgctgctaa caagtgccga aatgaccgcc
    46981 aaatgggaaa gttacttatt aaagattggt gagcagaaag gaacacaagc tactttctta
    47041 gctaatatcc aaaaatttgt ctctcacttg ttggaagtcg taccgggaca aatccagtca
    47101 acagattttg gatcaacgtt acaagaggtg aaagcagcca gtgaaaaaca ggaagcaacg
    47161 cgtcatttag gtgtttgtcc aaaatgtcgg gaacaagaag tcttgcttta tcaaaatgtg
    47221 gcggcatgta cgtctgaagc ctgtgatttt aaattatgga caacgattgc caaaaagaaa
    47281 ctcacagcca cacaactgaa agagatcatt caaaacggcc gaacaagcca atcagtcaga
    47341 ggactaaaag gtcagaaagg ttcgtttgaa gcaacgatcg ttttaaaaga agactttaca
    47401 acaggctttg aattttctga aaagaaaaaa acgaactata agaaaagaac acgccgcact
    47461 acaaaataat gaaggggtta tcctatgcga aatacaacac acaaacaaca agaaatcgaa
    47521 aatcaaaaag caaaaattcg tttagaacaa gagaaattac gtgcaaataa aagaagactg
    47581 caacaattaa agaaccagca gcgcattcaa aaagaaatcg aattagatcg atagacgtta
    47641 atcttgttac aaataaacta ctatgatata tttgatgtag tgtatttgta acgaagggag
    47701 gggaagtaaa tgcaagataa actgaaagaa ttgttggcta ttcgcgatgg aaatttatct
    47761 atgaaggaag ccaggaaatt tggaatcgca gcaaccactg tacaaagatt ggtgaatcaa
    47821 ggaaaattga tcaaagttga tcgtggttat tatgtagagg atggtcatgg aatagatgat
    47881 ttattttggc ttcagcaacg attttccagg ggaatcttct ctcatgaaac gactttagat
    47941 atttaccagc tttctacgaa tatgccgaaa ttcattcact taacttttcc ccatggctat
    48001 aacgttgatc gttcaattac aaaagagcag cagttacaat ttcatttcgt gaagaaagaa
    48061 gtttatgaac taggaaaagt agagggaact tcctttcaag gaaatcctat tactatgtat
    48121 gataaagagc gtacactttg tgatatttgg aatccgagat atgaatttga atatgaaatg
    48181 aaattagaag cattaaaaga gtacatggaa gacccattga gagatccaag taaattaaga
    48241 gattacatga caaaactaca tgtagaaaaa gaaatgaaat accacatgca aagcttatat
    48301 tgatggagga aaaagatgac accgacacaa tttgcagcaa aaacaagaaa tttagcaaag
    48361 agtacaggat taaatgcaca gttagtgcaa cggcatttta tgatggatcg tctaattgaa
    48421 caaatttctg aatcaaagta caaaaatgac ttcattttga aaggcggctt tttattagga
    48481 tcaaaatatg gaatcgatag aaggacaaca attgatatcg atacgacatt tagaaatagc
    48541 aaattaactg aagaaaaatt gaagagcatt tttaatgatt taactagaaa acccacaaaa
    48601 gaaggcattg ttttctctat tcaaggaatg aaggaaacaa gagaagccga tttttatcct
    48661 ggatttcaaa ttaaattggt ggctaattta gagaaagtac gtgtaccgtt taaaatggac
    48721 gtgacaacag gagattctat ttatcctgaa gtagatacac actatcacca attaatgttt
    48781 gaagataaaa aagtggagat tccagcttat ccaacagaac aaattattgc agaaaagcta
    48841 agtgctacgt tcagctttgg cacagataat tcgagagcaa aagattacta cgacttattt
    48901 accattccga aactagaaaa tatcgatacg aaagagttat acaaaagtgt tcgaaataca
    48961 tttacaaaac gtggggatac aaaaccgttg tccttttatt atgaaagaga aatggataag
    49021 atcagaggaa atgactattt ggaaaagcag tgggaaaaat atcgctcagt taacacgttt
    49081 gcgaattcca tttcatttac agatacagtc aacgaaattg atcacctaat gaaaagtgtg
    49141 atcaaaattg aaaatcaaga aagaaaacgg cagtttaaaa gatcacaaca aatgaatcaa
    49201 gaaatggaga gataaagatg atcaataatg taactttagt aggacgctta accaaagatc
    49261 cagatttacg ttataccgcg agtggtactg cagtagcgac ttttacccta gctgtcaacc
    49321 gaaatttcac gaatcaaaac ggcaatcgag aagctgattt tattaactgt gtgatttggc
    49381 gcaaaccagc agaaacgatg gcgacattag ctaaaaaggg cagtttgatc ggcgttgttg
    49441 gacggattca aacccgaacc tacgataacc aacaaggaca acgtgtgtat gtgacagaag
    49501 ttgtggccga taatttccaa ctattagaaa gtaaagcagc gacagaaagc cgtgcgcacg
    49561 ctgatcaatc gtccagcagt ccaagcccta ccacctttga acaacgggat gtagctacgc
    49621 ctaacaacaa tggattgaac gcttcacaaa atccatttgg cggacaatca atcgatatta
    49681 gtgatgatga tttaccattc tgattgtaaa agatcaataa agttgttata atggatacga
    49741 aaagaggagt tgcgctaaca actcccctaa cagcaccgtt taagacggtg accagcttaa
    49801 tattattttt gaaaaataat cgtcgaccgg ctaaagctga cggttatttt ttttgattgt
    49861 tgtttacaac tgctaagatt ccaaaaatga ttgttgcgat caaagaaccg aaactaatca
    49921 ttaatcctag cgccgtttca acaggcaaaa ggcttctcct ttctgtaaga ttttggaagt
    49981 acgcacatac gcaccacctc actttcaatc ggatagccac cgtcacaaac tttactgtct
    50041 tgatcattat agcatatcta aaatcgaata aaaattctca attttagctg tttttgctat
    50101 aattttagcg aggtgatcgt gatgaacaaa tatgttggtt tgcttgataa aatccgagta
    50161 ataaaaacac aaccattatt ggttcgcttt actttacaaa caattcatga atcgattaat
    50221 tgtgtagttg cagatattga aattatcgat aaattgttaa ttatggatga tggaaaatac
    50281 aatattgccg tcacaggtca tttcaataaa cggaatcaat tagtcattga atcgatgcaa
    50341 attagaaacc ccgatcattt tacgcgatcg atgggcatat aaaagaagag ctgcagtcct
    50401 aaatgaggat tgcagctctt tttgttttac atagaaacag gaaaagacct ttttaggaag
    50461 acacaagtgc cttctcaatt gaaaaacctt tctctcactc caaccgccac cgccgttcga
    50521 gaagaaagga agtgaacgtt gcacgttcac taagatatgg tgtcttgcaa agcaagacaa
    50581 aatagaaaaa acttcctctc accaaaaggt tcgttcctgt tcgctatctc tttattgatt
    50641 tgccagaccg gcaacaagtt gccttttatc accaaaaagc gacaaggaca agggtaaatg
    50701 gcacgctgcg cgcccttgtc cttgtcgctt ttcgttgatt cattctggcc aacaaatcaa
    50761 taaagaaaga actaacgaaa ggaaataaaa cctttcctaa gaggaggcag aatcaatcat
    50821 gcgaaagaaa tttgattatt ggggtgttcc cttcagcgaa ttattcccaa actatcatgc
    50881 accacatact gtcgaatgcg attgtggaga aagagcgaaa tgtatcaaaa gctatcgttt
    50941 gtatcaatgt ccaacatgcg ggaaaaaata taccttgtct tatggcgatt atgttttgat
    51001 tgatgagaaa gtaaagaaac ggtgatgaaa acacaacaca aattattgac tgtaaaagaa
    51061 gtcgttcaaa ttttggtgga caaagaagtc atttctgaaa aagtagcaga agctttactt
    51121 attccgcggt actggacgat agaaacccct ttagtagagg atgcaccgac tatgctattt
    51181 gaagacctag tgaagattgc aggatatgaa ctgcattatc aagaaaacat ggaaacttat
    51241 gaaatcgaat attggttaac caaattagag aactaaaaag gagaaaaaat atggctcaaa
    51301 attactatga cgaatttgtc aaattacctt tggataaaat ggcacaaaaa atggaggata
    51361 tgacgttcct ataccatgaa acacgtgtac ccaaaaaaca ttacaaggaa aaattgtcag
    51421 tcgcagtgga agaaatgatt gaatcaggtg tcgaaatgaa tctcattgcg acgtactatc
    51481 gaacactgga agaactgaaa aaacaaaatg gaaaatggtt tttccaagcc ttactatgct
    51541 tggaagtagg tgtcaaacca agcactatca aaccttccga atatcaagcc ttagaactga
    51601 cttatgcaaa attcgcacag attaagaaag ctaaagcaat ggctagcgac tggctagata
    51661 cttttgaaaa tatcgaaaaa aatggtgcat tttatacgat tgaatcggag gagaaagata
    51721 atgaataagg tggtttatca gctaacaaca gaagatgttt cgcaattttt aacaaacaaa
    51781 gaacacttga atgaagtaat cagacggatt caaaaagact ttagttgtat ggaagaaatg
    51841 aagtacttta ttcaaagagt ggaagaacag ttgtatatgg aaagtgaaac gagtcaaacg
    51901 aaggttacag tcagccataa tcgtttagac gtgtataaac gtcgtcctga cacattaaaa
    51961 aaaggagcgg agcttctacg actagaaaca gaaaaatata tcatgaatgt ttacctagaa
    52021 aggagtgagc tggtcgaaaa ctttgtcagc tacacgattt gcaggaaaca ctattactca
    52081 gaatcagaat attattcaat ttttgagagc atggaattaa tggaagaaga aatggaagag
    52141 ctaaacagca aagatgcgac ggtagacgat tattaccttg tatttactag atttttgacg
    52201 tgtgggacgg aatctatcgt gttaatggga atggattatg aactcactgt acccgaagca
    52261 tttttgaggg aatatgtaga agaagtagat aacccaggcg acgactggta cacgacttta
    52321 actgattttt tagacaccta cacttacgac gatacagaag aaatccaaaa tgaacttgac
    52381 cgtaaacgaa tgccatatca acgatttgaa aggaaaaaag gtcaataacc atttataccg
    52441 aaaagtgagg atgtgtgaaa taagaacaaa aaaccttgaa attaagggaa tggttcttat
    52501 ttcgcaccaa tcgatccaac cgcccccgcc gcttaggtgg tgcgaaataa gcgggcaaag
    52561 cccgataacc taagagaaag aaggagatcc atatgaataa aatcattcct ctattgttcg
    52621 tactgtttgt tggcgagata cttatgcgtc atggtgcagc tctacattta aacttcgtag
    52681 tattcttagg aatactggta ttttgtttga tcttttggcg ctttgtgtta acccttgtga
    52741 gtctaatatt caagttgatt gcagccatat ttttaatagg gttatttcga tgaaaggaga
    52801 gagtactaat gccaattgca ataggaaata aacggttacc cgtgacgttg gatgaaaaga
    52861 gacaaaaaga attgcagcaa ctaaagcaga agtacggcaa aagtgaatcc aggattatgt
    52921 gtattgcgtt agatttattg attgcccaag aaaaagcagg atttgaggta ccagcactca
    52981 aaaagtgacg tcacttttta tcctaaaaac taaaagtgat agcactttta acagaattag
    53041 gcagaagtcg cccatcctct ataggtgggt gatgaatgcc gttcggtatg agggttaccg
    53101 ttggtaactc gaagaccgac aactaaggcg tattgccttt ttattttgt
//
LOCUS       DAJSBL010000018        47952 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.018, whole genome shotgun sequence.
ACCESSION   DAJSBL010000018 DAJSBL010000000
VERSION     DAJSBL010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 47952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47952
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.018"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(38..1189)
                     /locus_tag="ONT85_001187"
     CDS             complement(38..1189)
                     /locus_tag="ONT85_001187"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HCR2378175.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(1202..1639)
                     /locus_tag="ONT85_001188"
     CDS             complement(1202..1639)
                     /locus_tag="ONT85_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCR2378176.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            complement(1654..3420)
                     /locus_tag="ONT85_001189"
     CDS             complement(1654..3420)
                     /locus_tag="ONT85_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HCR2378177.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEALATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            3684..4361
                     /locus_tag="ONT85_001190"
     CDS             3684..4361
                     /locus_tag="ONT85_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR2378178.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            4364..5491
                     /locus_tag="ONT85_001191"
     CDS             4364..5491
                     /locus_tag="ONT85_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR2378179.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLPLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(5691..6131)
                     /locus_tag="ONT85_001192"
     CDS             complement(5691..6131)
                     /locus_tag="ONT85_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCR2378180.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAKTIEEVQMIFEHGHI"
     gene            complement(6142..8175)
                     /locus_tag="ONT85_001193"
     CDS             complement(6142..8175)
                     /locus_tag="ONT85_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HCR2378181.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            complement(8177..9880)
                     /locus_tag="ONT85_001194"
     CDS             complement(8177..9880)
                     /locus_tag="ONT85_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCR2378182.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKKSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            complement(9974..10924)
                     /gene="manA"
                     /locus_tag="ONT85_001195"
     CDS             complement(9974..10924)
                     /gene="manA"
                     /locus_tag="ONT85_001195"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HCR2378183.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIATEPGK"
     gene            complement(10963..11988)
                     /locus_tag="ONT85_001196"
     CDS             complement(10963..11988)
                     /locus_tag="ONT85_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HCR2378184.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            complement(12197..13066)
                     /locus_tag="ONT85_001197"
     CDS             complement(12197..13066)
                     /locus_tag="ONT85_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extradiol dioxygenase"
                     /protein_id="HCR2378185.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            complement(13525..15417)
                     /locus_tag="ONT85_001198"
     CDS             complement(13525..15417)
                     /locus_tag="ONT85_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR2378186.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(15414..17141)
                     /locus_tag="ONT85_001199"
     CDS             complement(15414..17141)
                     /locus_tag="ONT85_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR2378187.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(17157..17807)
                     /locus_tag="ONT85_001200"
     CDS             complement(17157..17807)
                     /locus_tag="ONT85_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HCR2378188.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            18011..18307
                     /locus_tag="ONT85_001201"
     CDS             18011..18307
                     /locus_tag="ONT85_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HCR2378189.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            18479..19099
                     /locus_tag="ONT85_001202"
     CDS             18479..19099
                     /locus_tag="ONT85_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HCR2378190.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(19191..20357)
                     /locus_tag="ONT85_001203"
     CDS             complement(19191..20357)
                     /locus_tag="ONT85_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCR2378191.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(20369..20722)
                     /locus_tag="ONT85_001204"
     CDS             complement(20369..20722)
                     /locus_tag="ONT85_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HCR2378192.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLETVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            20850..21872
                     /locus_tag="ONT85_001205"
     CDS             20850..21872
                     /locus_tag="ONT85_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HCR2378193.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            21891..22052
                     /locus_tag="ONT85_001206"
     CDS             21891..22052
                     /locus_tag="ONT85_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2378194.1"
                     /translation="MNQQDVLDRLREELNIPFFDGKIEDKEYSEEEYQKLKADLQNYF
                     EQYVQNVEN"
     gene            complement(22311..22745)
                     /locus_tag="ONT85_001207"
     CDS             complement(22311..22745)
                     /locus_tag="ONT85_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2378195.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLKKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(22855..24714)
                     /locus_tag="ONT85_001208"
     CDS             complement(22855..24714)
                     /locus_tag="ONT85_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HCR2378196.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            complement(24832..25992)
                     /locus_tag="ONT85_001209"
     CDS             complement(24832..25992)
                     /locus_tag="ONT85_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-3-phosphoglycerate dehydrogenase"
                     /protein_id="HCR2378197.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            complement(26299..27723)
                     /gene="araA"
                     /locus_tag="ONT85_001210"
     CDS             complement(26299..27723)
                     /gene="araA"
                     /locus_tag="ONT85_001210"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HCR2378198.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(27739..28437)
                     /locus_tag="ONT85_001211"
     CDS             complement(27739..28437)
                     /locus_tag="ONT85_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HCR2378199.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(28394..30055)
                     /locus_tag="ONT85_001212"
     CDS             complement(28394..30055)
                     /locus_tag="ONT85_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HCR2378200.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKR
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            30273..31673
                     /locus_tag="ONT85_001213"
     CDS             30273..31673
                     /locus_tag="ONT85_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HCR2378201.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKVLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIIGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(31925..33004)
                     /locus_tag="ONT85_001214"
     CDS             complement(31925..33004)
                     /locus_tag="ONT85_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR2378202.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(33090..34049)
                     /locus_tag="ONT85_001215"
     CDS             complement(33090..34049)
                     /locus_tag="ONT85_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HCR2378203.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(34061..35572)
                     /locus_tag="ONT85_001216"
     CDS             complement(34061..35572)
                     /locus_tag="ONT85_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HCR2378204.1"
                     /translation="MKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHETADG
                     DGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIEPNSF
                     GLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQKKPF
                     GVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPRLPSY
                     PEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFDRQIE
                     EIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMEDTLLF
                     GSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAKGQVL
                     KQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLLDQGE
                     HYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(35594..36157)
                     /locus_tag="ONT85_001217"
     CDS             complement(35594..36157)
                     /locus_tag="ONT85_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HCR2378205.1"
                     /translation="MKIVETKFYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(36163..37026)
                     /locus_tag="ONT85_001218"
     CDS             complement(36163..37026)
                     /locus_tag="ONT85_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCR2378206.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(37040..37936)
                     /locus_tag="ONT85_001219"
     CDS             complement(37040..37936)
                     /locus_tag="ONT85_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCR2378207.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(38111..39400)
                     /locus_tag="ONT85_001220"
     CDS             complement(38111..39400)
                     /locus_tag="ONT85_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR2378208.1"
                     /translation="MKEEQKMKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTL
                     TFWNGFTASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFI
                     ALNYPDFAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMY
                     MYWNNDLFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLA
                     NGGKLVNDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIE
                     INGPWLNNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFL
                     KYWNTTEIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQIS
                     AINNDIINPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(39372..40889)
                     /locus_tag="ONT85_001221"
     CDS             complement(39372..40889)
                     /locus_tag="ONT85_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HCR2378209.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            41087..41977
                     /locus_tag="ONT85_001222"
     CDS             41087..41977
                     /locus_tag="ONT85_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HCR2378210.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(42050..42874)
                     /gene="nadE"
                     /locus_tag="ONT85_001223"
     CDS             complement(42050..42874)
                     /gene="nadE"
                     /locus_tag="ONT85_001223"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HCR2378211.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(42886..44358)
                     /locus_tag="ONT85_001224"
     CDS             complement(42886..44358)
                     /locus_tag="ONT85_001224"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HCR2378212.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(44995..45357)
                     /locus_tag="ONT85_001225"
     CDS             complement(44995..45357)
                     /locus_tag="ONT85_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HCR2378213.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            45580..46332
                     /locus_tag="ONT85_001226"
     CDS             45580..46332
                     /locus_tag="ONT85_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HCR2378214.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWLSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(46472..47395)
                     /locus_tag="ONT85_001227"
     CDS             complement(46472..47395)
                     /locus_tag="ONT85_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR2378215.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYQKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(47410..47682)
                     /locus_tag="ONT85_001228"
     CDS             complement(47410..47682)
                     /locus_tag="ONT85_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2378216.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
BASE COUNT        13979 a         9954 c         8268 g        15751 t
ORIGIN      
        1 aaaaatttcg tcacattccc ctttttcgta tcctatttta agcgacttgc ttggcataac
       61 ggttcacgtt ttgcttgata ttcccgattg ttttcggatc attgattcct gtgacttctt
      121 gaatcaattg ttccaagttt tcatggatct tcatttcatg caattgacgg ctttgttcat
      181 cttcaggatc atagtaatta aaaataatcc ccattgttgc cgtcaaatgt gggcaagtca
      241 agttatgttc ttgtagttct ctcactggac gtgtaaatct ttcttgataa cctaacttac
      301 gtaatggtgt ccgagcgaca cgtgtgatcg catcagagat atttttgttt tggaagcgat
      361 gaatgatttt ttcaatatac gcatgatgct gctctgcatc aaacccccat ttggcaatca
      421 gcaaacttcc tgtttcctgc aaaactgatt ttaattgtgc cacaactaac gcatcttgca
      481 ttgcttcatc aatggtctgg taacctaaca aggcacctgt ataagcgaca gtcgcatgac
      541 cagtattgac actaaatagt ttgcgttcga tatatggttc tagatcatct acatataaaa
      601 ccccttttaa ttggattttt ttattttttc tttggctatc gtcaatgacc cattctttga
      661 aaggttccac ttggacgaat aaaggatctt catggtgttg taatgggacg attcgatcga
      721 ctgctgcatt tggaaagccg atatatttgt cagtaaatga tgtttccgga agatatttat
      781 acacttcttt ttctagaaat gctgaaccgc caatcatatt ttcacaggca atgatatcta
      841 aagatgttgt gcttccttcc ttttctcttt cttggatacc ttttgcaatc aactcagcaa
      901 taaaaggtaa gatattcggg ccaatggcgg tagtaactaa atctgcttca gcgatttctt
      961 ggattacttt ttctggattt gtttgattgt tgattccttt gacatttttt acagtgattt
     1021 tttcttttga ttcatctgct aattcgattt cgtattcttt tctttcattt aaagcatcaa
     1081 tgatcgtgtg attcacatct acgaaagtaa tctcaaatcc attagctgcc aaaacctctc
     1141 caataaatcc tcgaccgata tttcctgcac caaaatgtac tgctctcatt tttctctctc
     1201 cttaagcaat tgctagattt tccgttactt cttctttgct gagcgcgtct gctagctgga
     1261 caacattgtc catatcactg caataaagtg cgatttgctg tacaagttgc aaatgctcat
     1321 ctccgactcc tgcaataccg aacaaaacag tcgcaatctt ttcttcttct tcggtaccaa
     1381 aatctacacc atctggtact tgaacgacac agatgccgga ttttttcaca tatttttttg
     1441 cttcatctgt tccatgagga atcgcgataa aattccccat ataaacagat aacattttgt
     1501 ctctatctac catggcatcg atatattcct catcaacgca ccctgcttct acaagcatct
     1561 gaccgcaata gcggatcgct tcttctttgg ttttaaaaga tcgattgagc tcgatcatgt
     1621 cgaatggcaa actttgcatg ctgttttcct cccttaagct gtctgtttca aatattgtgc
     1681 aatcgattcg tatttttgcg gattgaccat gctgttgact gataaatgaa tggattgagg
     1741 tgcagccatc tgtacttttt ccgctaaact ttctttggct acgatcagtg aatcaggttc
     1801 atcttttaaa tcttggatat tgacttttct taacgggata gttaaatggt tcttgtctaa
     1861 tatatctttg agcacggtca tccccattgt tgctgatccg gaacgatcat cgtggacaag
     1921 tagaatctct ttgatttgtt tgatgtttgc tgaaagaaac tcttctgctg gctcattctc
     1981 aatttcagaa actgatgttt tctgcttgct gcttaattct tgaatgattt catcatatcg
     2041 aggtgaattc aaaaagtttt ctactgagac aaaacgcgcg ctcggtgctt tttgttttgc
     2101 tctttcttgt agttcgacct gagtaacgat caatgtattc gagtcatctg tcagttggct
     2161 gattgcttga ttggtcaccg tttgaggcaa gtctgcttcc tgtacttttt ttctcaagat
     2221 cgaagcgccc atcgcacttg atcccattcc tgcatcacaa gcaaaaatga ttttttgtac
     2281 gccagagaat gtagctcctt gaggaatacc ttgggcacct ttggaagctt gcttagctgc
     2341 ctttgtctct tgaacagatt tttcgaagtc ttcatttgta tctttcgcat ccgctttcaa
     2401 aattactgca gaaataccaa acgaaacagc tgcggcaaca atcacgccca aaataacagg
     2461 aaggtaagaa ttgaacggtg tcatcgctag aatcgcaatg atcgaccctg gagaagctgg
     2521 cgctcttagt ccagcatcca tcaattggaa aatgaaactt cctgacattc ctccgcctat
     2581 aactgataag aataataacg gtttcatcat aacgtaagga aaatagattt catggatccc
     2641 gcctaaaaag tgaatgatca tggctcctgg tgctgaagat tttgctgctc ctttgccaaa
     2701 caaagtaaaa gcaagtaata cgccaagtcc tggaccagga tttgcttcaa gcaagaatag
     2761 aatagatttt ccagcctctg ctacttgttc agttcccaat ggtgtcaaaa tcccatgatt
     2821 gattgcatta ttcaaaaata agatttttgc tggttcaata aaaatacttg tcaatggaat
     2881 caaatgggca gataaaatga tctctacacc ttttgccatt gcttgagtca atgcatcgac
     2941 aactggacca atcgcagaaa aacctaataa cgctaatcca aaaccgataa gacctgctga
     3001 aaaattattg actagcattt caaaaccgga tttgatccgt ttttggaata gctgatcaaa
     3061 tttcttgatg gcatatccac caagaggtcc catgatcatc gcgcctagca tcattggaat
     3121 atctgttccg acaatcacac ccatcgttgc aattgcccca acaactgatc cgcggtcacc
     3181 agcaattact tttccgccag tataaccgat caataaagga atcagataag ttagcattgg
     3241 gcttaccatg gtagccaaag cctcatttgg taaataacca tctgggataa atagagccgt
     3301 taatacaccc caggcaatca gtgctccaat atttggcatg accatgcttg ataaagtact
     3361 gcctaatttt tgtaccctcg cttttagcga aatgttgttt ttgggaatcg tccgttccat
     3421 tttgtctctc cctctctcct tttgatactc taattgtatc cgcttactaa aaagagaaat
     3481 agtcgtactt tggcacgaaa ctttgtgcca aaggtttgag tatgtcagca aaataaaaag
     3541 atttcggtat cagaaaactt caattttaaa taaaactgtt attttaattt ttttataata
     3601 tgcaaacaat aacttttgtt agcatttaat ttagatgttt tccattctat tatgttatta
     3661 aattttatat tggaggccta tctatgacat tactgatcaa aaacatgaac atgaaacgtg
     3721 gtaaaaaacg tgtgattcga caattgaatc ttttagtaga atcaggagaa gtagctgctt
     3781 tgattgcccc gaacggattt ggcaaaacaa ctttgctgaa tggcattgcc caactgctgc
     3841 caacaactta tgatcaattg tcccttcaca acatctctcc tactgcttat acagattatc
     3901 gccggtcttt cttttttcta gaatcaacga atcagttatc cgactatcta tcggctgttg
     3961 attatttgac tttgatcaaa tactattggc agagcaaagt agaatgccaa actgttttcc
     4021 aacgattgaa gatgactgag taccttactt tgccgattgg aaaaatgtct ctaggaatga
     4081 aacaacatgt cttgcttgct gcttacttga tcagtgattc atctgtcatg ttattcgatg
     4141 aaccgttgaa cggattagac ccaggaagta ttgatatctt gaatagcctc atcagacaat
     4201 ggcagaaaga ggggaagacg attcttcttt cttcccacgc tatcgcgaat atccaatcga
     4261 tttgttctaa agcatgtttt ctaaaaaatg gagaaatcgt cacagaatcc tcaaatatgg
     4321 aagagatttt agctatttat cgtaaattat atgaggtgta actatgtctt tacttacatt
     4381 tgaacgcaat ctcttactaa aaaacaaagt aaacttttta tttcctctat tgttggtcgt
     4441 gctctttgca ttcccacttt tttttgatca taaacttgct tatacagaat tcgatgaact
     4501 gaatcataac tatgaagaga tgcaacgttt gattgaaacg ttgaaagaag atgagaatga
     4561 gaaagaattt gtggaaagtt tagagaaaag caacaaactg atcgaagcaa tcttacatgc
     4621 aaaaaacaca gggaatgtcc agcaaatggt cgaagcaact tatcattatg aaaaagatat
     4681 actggatcga ctgatttctg gtcaaagaca aggaattcca atcattgaac aacaaaaacg
     4741 tgtcgagttg ctccgttata tgaaagaaca tcagatccaa agatattcta tttttgactt
     4801 gccagcgcat ctccctttag caaattacta cgaaaatatt ttttcaggaa tgatttctag
     4861 ttttttgatt ttatgtataa ccgccttgtt tctttcttcc atcatctctt atgaaaaaag
     4921 aaagcaggta atctctctag tcaatctatt accggatagt atggtaaaga aacatagcat
     4981 ccgttttacc atttattatg gtgcagctat gctaagtctc gtgatgccat ttttgatcgt
     5041 ctctatacta gtaataatca aaaatggttt gggagacttc cgttatcctg taggaacgat
     5101 cattggtcag gagatacgaa tccttccgat gtatgagtat cttttccaga gctttctgtt
     5161 cctgttgttg tgggtgctct ttttaagtac aatcagtttt cttctatctg ctttatttga
     5221 acattctcta gtcaatcttt taggtacact tctttgtctt ttcttagcag agtatcgctt
     5281 gttttcttcg attggatgga tagaatcaat cagtcattac ttgccaactt cttatgttga
     5341 ttttcaaaat gtgatcatag gaggagatat tttctcgcca ctagcttcag aacaggttac
     5401 tttcatgaat ggtatcttga cacttggtat atggagtatc gttttattat tcataggtat
     5461 gggcactatc tatataaaaa agagctatta aaacacaaaa ataatagggg cggtgacaaa
     5521 aaccttgtca ccgtccccta tttcaaataa acggttttct agaataaata gttttcaaaa
     5581 gcttgcatcc gcagtaataa gaacctccca acaaaaatag ctcctcttat ttttatcttg
     5641 gttgaagtta ttacttgatg caaggcagct ttttttactg tgaacattgg ttatatatgt
     5701 ccatgttcaa aaatcatttg gacttcttca atggttttcg catcactgag cgccattact
     5761 tggtcgatgt ctgaacagaa aaaggctaaa tcttggagca ccgctaactg atgttctttt
     5821 tttaaagcca ctacaaataa gagtgtcgcg atttttggtt cctcttttgt tccaaagtcc
     5881 acaccatcag gcacttggac taaatagatt ccttcttcta ctacttcttc atctttgtca
     5941 gtcccatgtg gcaaggctac gaaattcccg atatagacac tcgctttttc ttgtctttcg
     6001 atcattgatt ggatatatgc cggtttgatt cccccttgtt gggtcatcca ctggccgatc
     6061 tcggtcaacg cttctttcca attttgatag gattgatcga gcaatatttt ttctgctggt
     6121 agctgtatca tcttttcacc ttcatttctt tatttcttca atcaggattt tcgctaacaa
     6181 ctgataaacg atttctttat ttcctgagcg gaagatctcg gtgttcaagt cattcatgat
     6241 gattgctcca ctgatctttc ctaaaagaat cccgtctttt tcttggattg gcgaaggcgc
     6301 cagcataact aacatacggg acaaatcgac tggctgtcta tccatccctt caatggtcaa
     6361 cggatgtttc aaattgaaaa tacaaaagat cggtttagaa atcaatgagc tggatgtatg
     6421 gaataacccc atatttgtat gaggaatacc gataggtgct tgttcatatc gcttaagcag
     6481 ccggcgatga accacctctt catccgttac tagtccttca ggaaaataag gaaataagct
     6541 gcgaatcgtt tcggaaatcg tctcaggatt atccagttct tggataaaaa aacgaccgag
     6601 taattgattg atttgatcca tgaaatgcat tgtttcctca taagtatctt ctatcatcca
     6661 ctcgttattg ctgttatacg taattttttg cctttttttc ttctcaatat gcgaaaaagc
     6721 ttcttttagc tgcttgactt catcttctag cagtagcggg gagactaaaa tatattttcc
     6781 accgtatcct ggtaaaatag atgtagaaat aacaacatca tattcttctt gtaaatcgat
     6841 tttttttaaa tcagcaatcc gatagaaatg aatttgttgg atgaagggaa aatacctttt
     6901 caatcgtagt tccaacatgc tagtagaagc taatccactt ggcgaaaatc ctgcaatact
     6961 gatattgctt tctttcggac ttcgttccaa tgaattcgca aaatgaagga ccatataagc
     7021 gatctcttct tcggataatt ttttttcttg aaaaatccgc tctactgctt tacggattgc
     7081 attcgcaact tcctcgtatt gaaccatgat tttttctaaa atcggattgg tcagtgtctc
     7141 ttcttgtaaa acggctcttg aaaacacacc agataaatga gtcaacagca ttttgtacaa
     7201 agtttcatct tcataaaaat ggacattcgt ctcttcactt acttcttgaa tcagttgttt
     7261 tactaaataa gcaagctgtg tatcgaacgt ctcttcaaag aaattctgat caaatgagtt
     7321 cgtgaagtca tttaataatc ctgcaaaaaa gacgatctca tttactggat aaagttgttt
     7381 tgtctcagaa gcgatccgtg aaaacaatcg tttagctgtc tgtagtaatt ctttgtttgc
     7441 cagactatta tatgtctcat ttgtcagtaa atgattttta gtcacacgct ccatcgtcaa
     7501 tgtcagcatc aaaaccaaat gttcaagctt tctatcactt agattggtct ttttttggat
     7561 caattcttcc ccgattactt tgcgtgcaag agatagatgt tgcggatcaa cgaatcgaaa
     7621 aaaataattc agtgatgcat cgaaagaaat actatgaaaa agttcatatt cattgatttc
     7681 taattctaat acattcgctg tcagaagccg gcggtatttt tccggtcctt cgatctcgta
     7741 tccctggttc cgaatgattt ccaaaggcag ttggcgaata ctttcttcca actgtttgat
     7801 atctgcataa aaagtggtat tactgatgcg atatgattcc atgaaatgag caagactcaa
     7861 cggctctgtc gtcagtaaca actcggctaa aatcgcgtgt tgtcgtttag cagtggataa
     7921 gtcattactt ttcccatctg tattttcttt taattgctct aatacttctt ctgatgcctc
     7981 aatgacaaat gagcctctag ctgctttttt caaagaagca tttaccgatt tcaatgagac
     8041 ttcaagatta tctaattcgc ggtaaatagt ccgtttactg accttcaata aattcatcat
     8101 tttttggata gatacaggtg acgggctact gagaaattct gctaataatt gtttttctct
     8161 gggcgataaa tacatcttat atttctccct tcacgagttt ttcgtattca ttcgtagcta
     8221 cccaatgttc aactagaact agccgaggga tcttttcttc tagctgttca gttacagcta
     8281 gttctctttg tgcgatataa atcgtttctt cttggatttt gattttttct attgattctt
     8341 ttgagataac aaactgtttt ttcgcttttt ttgctaattg acttaaaatc gtcatgccca
     8401 tagtttgtga gccgcgtctg ttgtttttat acaaaaatac aatattagca ggcgactttt
     8461 tttgttctgt gatatctgct atctcttttt tttcttcccg ctttggaaat tggatcgctt
     8521 cttttattcg tgacagcaag atatctgttt ccaaaaagtg atccagtaca tagatttgac
     8581 tttgaggtac tttttctctt gcaagacccg ctagttcatg ttgcacgata accagctgat
     8641 ttacttggtt attcaattca tagatagagc tgtatccgac agaaagatcg attttttctg
     8701 ctgctaattt ctttttcagc agggcggctc ccatagcact tgatcccata cctgagtcgc
     8761 aagcaaaaat gacggaatga atgatagatg gttccaagat ttctcccctt tctttgaccg
     8821 ttttgttctc tttttcttta tttggtaatt tcaatagtat cgctgcacaa atgaaggtca
     8881 caaatgtact gattgctatc cctatactta ccccagctag ttgtggataa ggcgcattgg
     8941 caaaaatcac gagtagtgac ccaggcgata caggtgcagc taatcccgta tctaaccatt
     9001 gaaatatcaa cgtgccgctt gccccaccag ccattacagc taaaaataga tacggtcgca
     9061 gcaagacaaa tggaaaatag atttcgtgaa tcccgccaat cgcatggatc atcaatgctc
     9121 cactcgcatt tactttttcc tgtttttctg caaaacaaag gatagaaagt aagatcccta
     9181 aaccaggtcc aggatttgcc tccatcaaaa ataaggaaga gtacccgact gtttgtgaga
     9241 attcgattcc taaaggtgtc aatacaccat gattgattgc attattgaaa aatagtactt
     9301 ttagaggttc taagatcaga ctgacaagtg gtaacagatt cattgtgact agccaagaca
     9361 caacggaggc gagcaacaag tgaacactat ttaaaactgg ttcaataaaa acgattccaa
     9421 ttatacaaat aacgctacca gtcagtccta ctagtaggtt gctgacaagc atttcatatc
     9481 cagccttgaa tttcacgtgg aatctagaaa caaattgttc atatacgatg ccggcaagcg
     9541 gcccgataat catcgctcct acgatttgtg gagcattgga acgagcgatc aaccctaaag
     9601 aagcaattgc accggcgact gccccttttt gtccccctac cgatttcccg cctgaataac
     9661 tgatcaagat gggtaaaagc agctggatca tccaatggca tacctcttct aatgtggtgt
     9721 gcaaatcgcc agacacacct tttgttgcta atgacaagat tccccaagcg ataaacagac
     9781 ttagatttgg catgatcatt tcatttaagt gatgtccagc ttttctcagt tgtttcatag
     9841 acacgcgact gctgttgttt gctttccatt tgtattccat ctttttcacc atacttctat
     9901 ttattgatgg acggatttta tgtagaaaaa gcagcaaacg aaaaaccgtc tgctgctttc
     9961 agctatctgg gaactatttg cctggttcag tcgcaatgat tgctagttca ccttggaccg
    10021 cccactcaga aacgccattt ggaataatac agcttgttcc tttttctaat ggatatgttt
    10081 ttccgtcaac gacaaggtct cctgctcctt cgattaccgt catcaatgta taaggggctt
    10141 gttttttgaa agatgtgatt cctttaatat cccactcata tacattgaaa aactcagttt
    10201 ctaaataagt gacgatcgat gaattccctt ttcggacttc tttgatttgt aattctggtg
    10261 ttttcgcagg gacagttgtt acatcgatcg attgttggat atgcagctct cttgtttgtc
    10321 cttgcgcatc ttttctgtcg tagtcataca cgcggtaagt cgtgtcagaa ctttgctgtg
    10381 tttctaaaat catgattcct ttaccaatag catggatcgt tccgcttggt acataaaaga
    10441 aatcaccttt tttaactggt actttcttca aaaggtcatc ccagcgccca tctttgatca
    10501 tctcagctag ttcttctctt gtctttgcat gatgaccata gatgatttct gcaccaggtt
    10561 cagcatcgat gatataccag cattcagttt ttcctaattc accttcatgt ttcatcccat
    10621 aggcatcatc tggatgtact tgtacagata gatcatcttc tgcgtctaag atcttgatca
    10681 ataatggaaa cactggttct gaaggatggc caaacaattc cttgtgttct gaccatagtt
    10741 catctaatct tttccctttg aattcgccat tttctactac actgacacca tgaggatgcg
    10801 cactgatcgc ccaatcttct ccgattttat cgttagggat atcaaaacca aagactgaac
    10861 gtaaacgact tcctccccaa attttttctt gaaaaactgg ttttaaaaac ataggttcct
    10921 gcataattca aactcctctc atttttcata gctattatag cattatttcg gtaataccgc
    10981 tttctctttc aaaaagtttt caacaatttc atctcgacat atgagatact ccgcacgatg
    11041 atcgacagga tgtacgcgat ttgacaaaaa tatcaacgct tcttgtgttt ttatatcaat
    11101 caagaaaaac gtacctgtat aaccagtatg aaaaagaatc gcatgaccgt cttctacatc
    11161 cttttttaag tcccatccta aagaacgata gccatttttt ttcggtgttt gatcttgcag
    11221 taaagaatca atcgtctctt cttctaaaaa acgaccatta cggatttttc ccttgttcaa
    11281 atacatttgg acaaaagtaa tgctgtcttt caaatcaata aagagacccg cgttacctgc
    11341 atgttcagcc aaaactcttg ctttcggatc atgagtttgt ccacgcaaaa cctcacccga
    11401 aggcagttgt tctgtgggaa cgattttttt ggaaggaaaa ggaagaaata agctgttcat
    11461 cattcctaat ggttccaata cttgttcttg aaagatttcc actgcagttt cttggtaaat
    11521 ttcctctagc atcaacccaa gaagaatcgt accggcatct gtatatttta cttcttttcc
    11581 tagttgctca cctggttcta gcttcaaata agcggcgatc aattcttcct gagatagctt
    11641 atctctgttt ggtatccatg ttttgatatc agaagtatgt gtcagtaaat gacgcaaagt
    11701 gattgtctga ttttcaaatt caggaagata tttatgaagc ggttgatcga gttctatctt
    11761 tccatcttct aatagacgta aaactaatga agtggttgca acgactttcg tcagtgaggc
    11821 tgcatcaaac aacagatctt ctgtcatcag ctgcttttca ggaacgactt gggcaaagcc
    11881 ggctgtaaat gtttctactt gattttcttt tatgaaggcg actgctacac ctggataaaa
    11941 tccttcttcc atctttttca aaataagctg tttcgtgtgt ggatacatct ttttctcctg
    12001 cttttctatc agattcttac agtccaagct taaagcttaa tctatgagga atcaaccttt
    12061 tttacacaaa aaaactgcgc gaaaaaccgc gcagtttccc ccggtatact attttctctt
    12121 tctatctcta gaataagcga tagtatgacc gttcataaaa tgacgagaca tgcgacaatc
    12181 gttaagaaag aaaaagttaa gcgtcgcatc gaatgtcagt tttattcttt tcttttggct
    12241 cactgacgaa ccaccattcg attcctatag catcatagat tgtgagtact gtgtgctttt
    12301 tgtctaagaa gaaatcaact gatagttgct gcaagtgagc aatcagtcct tccatgcttt
    12361 cagtatcgat catgaatttc agaaaatcaa gtccaatcac ttcatctgta ggcaaatcaa
    12421 ttgcgcctcc gctagcttct gtcaaaccaa catggaaatc tccttcattc aatacatgga
    12481 tgccctcttc ttcattatgt accttcaaac caagtacatg attaagaaat tggcgttctt
    12541 ttgttaagtt agtaacattt aaatggattt tttcaaaata agaacctgtt gatagtttac
    12601 tgaatttttc ttcagatttt cttaataatg cttcttgatc cattgtttct ggaatttctc
    12661 tttggtcgtt cttattctct tcatggtaaa tttccaattc attctcttct gggtctacga
    12721 gcaaaatccc catacgtcca tgatcttcta aagcttcttt gattggataa ttcattttac
    12781 ggatacgaca aaggatatct gccatttctt ctgtgctagg gataatcaat gaaaaacggt
    12841 tcagcttttt gatcaccccc atgtgatcat tcgctctagg actttcttcc agcagcaaca
    12901 tttcacttcc agcttcttct gttcctaaaa tggctaattc gttttcttct cttttcaaat
    12961 caaagccaac aacgttcttg taaaaatcaa tcattttatc tcggtctttc acccgaatgg
    13021 ctacactttt taaataggtg gcagccccca attgaaaatc tttcatattt cttgctcccc
    13081 tctacgtcaa gctttatttt aacgatggat tcttcttctt tcaagcaata actttgattt
    13141 tgttaaaaaa aatgtgagaa aattcgcatt ttgcttgttt tttgtaaatt aatgagtaaa
    13201 aactcttata aaaagaggtt gtgaaaaagc tgttctgcat caagtaataa gaacagccaa
    13261 acaaaaatag ctcctcctat ttttcgtttg gttgggctta ttaccgcaga tgcaagcttt
    13321 tgaacaccgt ttaaggaaga ggttgtaaaa tcagcggttt gacctgagta ttagagatac
    13381 aaaacggaaa aatagcttct catattttgc cgttttttga gctaataccg aaggacagct
    13441 gattgaacac cgtttattcg gaaaaaggga ctgtgacacg acttttgtca cagcccctaa
    13501 aaaaaataat aaatttgtac tattctatga agcttcttct tcagaaaact gactgttata
    13561 aagatctgca tagaatccgt ctttttccat caatgtatcg tggtttcctg tttctacgat
    13621 cgaaccatga ttcatcacaa taatgttgtc tgcatcgcgt attgtagaca agcggtgagc
    13681 aacaacgaaa cttgtccggt tttccaatag gcggttcatc gctttttgaa tcagtatttc
    13741 tgttcgtgta tctacactag atgttgcttc atcaaggatc aggacatctg gatttgctaa
    13801 gaatgcccgc gcaattgtga ttagctgacg ctgcccttgc gaaatattgc ttgcatcctc
    13861 gtttaataca gtgttatatc cttctggcaa tttacgaaca aaatcgtcta cgtgagcagc
    13921 tttagcagcc tcaatgattt gttcgtctgt tgcatcgtcg ttcccatatt tgatattgtc
    13981 atagatactt cctgtaaaca gccaagtatc ttgcagtacc attgaaaact gtgcgcgcaa
    14041 ttcatcacgt gaaagatcac gggtatctac accatcgtaa cgaatactac ctccagaaac
    14101 atcgtagaac cgttcaagaa ggttgatcag tgtcgtcttc cctgcaccag tcggacctac
    14161 aatagcgacc atctcaccag gtttgacatc caagttaaaa tcagtcatta gcaagttatc
    14221 tggtgtgtag ccgaattgaa cattttcaaa acgaactttg tatggtgtat ctttttcttc
    14281 tggaaggcca gatttttcat tggacatctc ttcttcatcc aacacttcaa atacccgttc
    14341 ggctgaagca actgttgatt ggatagtgtt catcaaactt gcaagttgag tgattggttg
    14401 agagaactgt gttgtatatt gcaagaacgc ttgaacatcc cctaatgtca tgttcccatt
    14461 tgcaactttt actccgccta gtacagcgac gaacacatag cctagatttt taacgaaatt
    14521 catcaacggc atgatgattg ccgaaataaa ttgagctttc caaccagcgg catacagatt
    14581 ctgattttct ttttcaaata cttcctggtc tttttcagta tgattgaatg ttttaacaat
    14641 ggtatgacca ccatatgttt cttctacttg gttattcaat aaacctaaac ttttttgctg
    14701 atctgcgaaa aacacttgtg atttcggcgc tacgatcatc acgatgatca agcttaaagg
    14761 gatcatcaat aacgcgacta acgtcaattg ccagctgatt gaaagcatca tcacgatgac
    14821 cccaatcaat gtgactgcac tagttacaaa ttgagtcaaa ttttgctgca gcgtgcttgc
    14881 gatgttatcc atatcattga ttgctcgaga catgatatcc ccatttgtat gtgtatcata
    14941 ataggaaatc ggtacacgat tcattttggc ttccaaatca tgacgtaatt cataaactgt
    15001 tcgttgggaa acacgtgtca taataaattg ctgtaaaaag ttgaatacag cagaaatcaa
    15061 atacatcacg attactgtcg caatgatctg cgcgatttta tcaaagtcta tcggaaattt
    15121 tgttacttgt tggccttgtt gcatctgttg agcgccagcc attacacctt tgaatatttc
    15181 tgtggttgct ttccccaaga cctttggtgt ttggatttgg aagatcacgg ctgctattgc
    15241 tagtacaaat acagcgatga tcgcaaccat acgtttagac atatagccaa acaatcggcg
    15301 taccgttccc cagaaatttt taggcttttc accttttaca ccgagatttc tgccaggtcc
    15361 accatgtcct ggaccgcctg ctggtccatg agagggacgt gtgtttgttt tactcatgct
    15421 aagtcctcct ctcttagttg tgaatgaacg atttcttgat aagtttcgtt tgttgctaat
    15481 agttcttcat gtgttccttt gccaaccagt tttccttcat ctagcacaag gatcatatct
    15541 gcatccatta ctgtacttac tcgttgagca actaaaacgg tgatactttc tttcatattc
    15601 gttttcaatg cttggcgaag attggcatcc gttttgaaat ccaacgctga gaaggaatca
    15661 tcaaacacat agatatctgc tgttttgact aatgcccgtg caattgccag acgctgtctt
    15721 tgtccaccag agaagtttcc tcctccttgt tccacatggc tatctaagcc atcttccatc
    15781 tctgaaacaa aatctttagc ttgtgcgatt tctaatgctt tccaaatcat ttcatctgtt
    15841 gcatccggct taccatactg catattgtct cggatcgttc cagagaacag aactgctttt
    15901 tgaggaacaa agcccattaa ttctcgcaaa ttatattgag tcatgtcact aatgtccgtt
    15961 ccatttattt ggatcgaacc agattcgatg tcatataatc gagggatcaa attgactaag
    16021 gtcgttttac ctgaaccagt cccccctatg attgcgacga tctcgcccga cttggcttgg
    16081 aaatcaatat cttccaatgc aagtttttct gctccatgat atcgataatt gacatgattg
    16141 aaggccaatg ttgcgttttt tccagcaaaa gaaactgttt taggattttc agggtcatgg
    16201 ataccgattt tttcatccaa cacttcatta atacggtcag ctgaagcttg tgcacgaggc
    16261 accatgataa aaatagcgga aagcatcata aagctgatca agatctgcat cgcataagtc
    16321 ataaatgcaa tcagattacc gacttccaaa gtcatatctg ctatgtagtg tccaccaaac
    16381 caagtgattg ctacgtttgt accgctcatg atcaatgtca tcattggcat catcaatgcg
    16441 acgatcgtat taactttgat tgccgtattt gtataatctt tattcgctaa atcaaaacga
    16501 ttttcttcaa agcgtgtctt atcgaatgct cgaatcactc tgacccctgt caatccttct
    16561 cgaaatacca ggtttaaacg gtcagttttc ttttgcatac ttttgaataa tggaacagca
    16621 aagtacatga ttccgccgat caaaataatc attactggga tgacaactaa gaagattttt
    16681 gtcaactgat gatctttttg ataagcaagt atgctcgcgc caatcaacgt gattggtgca
    16741 ttgatcatca tacgtaaaaa catttgtgta accatttgga tttgagtcac atcattggtc
    16801 gttcttgtga tcaaagaggc cgttccatat ttatcaaacg catcttttgt cagactctct
    16861 gattttttat agacatccgt acgcaattgc ttacccagtt tctgtgattc acgagtagca
    16921 aaaaatgtat ttgcgatagc agctaagata ctgatcaaag aaaagccgat catcacaaaa
    16981 cctgtatgcc aaatataatc gacatctcct tgcgctaccc ctttatcaat aatattggag
    17041 gtaagtgtcg gcaaatataa atctgccagt acttggatca ccatgaaaac tgcagctgca
    17101 attgctgaag tcaaggagat ccgttttacg atttttagca tctagttccc ttctttctat
    17161 ttatatactc cgtttttcag ccaagaaagt ttcaaattga attcactgat tgcttgctct
    17221 actgagtaat cttcagatgt tgaaaataag cgatacgaat gcgcaattgt tgtaaataca
    17281 gtattcatca ctaattggat cagtaattcc aattcatgat cattttttac tttcagcttc
    17341 gtccgatcga tcaattcaag caactctttt gcatcttgtt tgtgttcttt atgtggatcg
    17401 tgaggcgttt tttggcgact cgtatgagga gcgacttttc ttgaggcacg gtaatccatg
    17461 ttcataaaca agttacgata aaaagaagca ttctcgcctt ccaaaatctc ataaatcatt
    17521 ttagaaaaat aggattcgaa tcctttgaat agatcaccat cggattcttt cattagttgt
    17581 aacatatctt tagagctgtc acgtctcatt gtctcaaagt aataaaaata aagatcctct
    17641 ttatcttcaa aatactgata gaaactgcct ctagggatat ccgctaattt gacgatatta
    17701 gcaatcgaag cttcttttaa gggtactcta gaaaactcga ttctcgcagc ctcgattagt
    17761 cttttttgtt tttctttagg caaatggaaa aatgtctgtg tcggcatatg gatcctcctt
    17821 tctttcaaga tgacacattg tcatatgacg ctgtgtcatt ataacacctc ctacctgttt
    17881 tgcaagacat ttgcatagtt tttttcatta tcatccgata cattaaaaaa gtgataagag
    17941 gaatccttcc atttgaatta atgagagttg cccattaaaa tagccacata ctaaacaaag
    18001 gatgagataa atggcccaat caattagtgt ccaagacttc aataaattgc ctctcgatgc
    18061 tgacagcatc gttttagata caagaaatac cttagattac acaaaaaacc acttaagcca
    18121 ctcgctttct atccccgctg ctatcttgcc aagacaatta aaaaatctag atccaaagaa
    18181 actttattat attctaagct atacaggcag gcgatcagaa gcattagccg atcaacttac
    18241 tgctaatggt tttcgtgctg tttatatcat tggtgggatg gaagctcttc agtcgagtgt
    18301 tgcttgaata ttgcttgtta gattcaaaag aggctgtgac aaaaaatcgt gtcacagcct
    18361 cttttatacc ctgtcttttt ttagttattt attcttcaac cttatcaata tgacttgata
    18421 gccttttctt tttctcatag ttataacaat catagcaata aaaggagaag gtaagcatat
    18481 ggcatctgtt ttagtagtca aaggtcatcc cctaactgca gaagaatcgc gcacagttaa
    18541 ggcattgacg tcttttttaa caagttataa agaaaatcat ccagatgatg aagtgactgt
    18601 tttggaactt tatcgcgatg atattccgga aatcgatgaa gaattattat caggttggga
    18661 agcattacgt gctggtgcag aattcacttc cttatcagaa agtcaacaac aaaaaattgc
    18721 tcgtttcaat gaattgactg atcagttcct tgctgctgat aaaatcgtta tcgcaaacgc
    18781 cttatggaat ctaaatatcc caacaaaatt aaaagcttgg ttcgataccg tcaatgtagc
    18841 tggaaaaacg ttccgctata cagaaaacgg gccagaagga ttagtgacag gtaaaaaagc
    18901 actgcacatc caatctaatg gcggtgtata caacggacaa gactttgcct ctcaatatgt
    18961 aaaaggtatt ttaaactttg taggcatcga tcaagtagat cagctattca tcgaaggaat
    19021 cgattatgac cctgaccgtg cagatgagtt gatgcaaaat gcattagata aagctgctgc
    19081 tttaggtaaa tcattttaaa aattgagata aatgcataca aaagagacag aggaaaaaat
    19141 cttcaaagca gcatttgtac cttggatatt ttcccatctg tctctttttg ttattctttc
    19201 tgctttttca acggattttc ttcataaaga gaagagattg ttgtgtacca ttcaaaaaca
    19261 tgagtaatca ctactttagc tgctgcataa acaggaatcc ccaagatcac accgatgatt
    19321 ccgaacaatt tcccagaagt cagcagaact aataaaatcg ttactggatg gattgctaac
    19381 tgattcccta gtactaatgg agagatcaag cgtccttcta ttgtctgctc tactgcaaaa
    19441 acgatcagta ctttgatgat catgaatgga cctgctacaa tggctaaaaa gatagccgga
    19501 atcatcgcta aaaatgatcc taagtaaggg atcaggttca aaaaacccgc aataatcccc
    19561 aacgtgacag catactctaa accaatgacc gcaaaaccaa tcataaacat gattgccaca
    19621 gcaaaagcca cagttagttg tcctcttata taagaagaaa cttgactatt catttcactc
    19681 agcacattca acgtcggttt ttgcatgcgt gtcggtaaaa atttgacaaa ataaggtgct
    19741 aattgtttac cgtctttcaa tagataaaat aagataaacg gcatcgtcag cagagccact
    19801 aaaatagtcg ctaccgcgcc gacaaaactt ccaagactct gaactgtact cttagatata
    19861 tcttggatca tatcgcctgc cgaattcatg aacttatcac ctgctgtttc caactgttcg
    19921 cgaaattggt tgaacagcgg gtcccgcaaa atctcggtta atttattatc aatcgtatct
    19981 acatattgag ggaagttact gatgaagctc actgtttgtt cctgaatctt agggatgatc
    20041 actacgctcc cccatgcgat tagtgcaaca accaaaacaa acagcccaat gatactgtaa
    20101 agccttggta cttttcgctt ttcaaagtaa tcaactaccg gattcatcaa gtaatacaag
    20161 attcctgcta aaatgatagg cagacctacg attgctaaaa attgccagat tggtgcaaac
    20221 aaatatgaaa cttttgtaaa taaaaagacg atcagcaata ttaacaatac aacaagtaag
    20281 gctgtgacta cttgattatt taaaaaccag cgccaaaacc acgataccgg tctgttcttc
    20341 ttattttttc cttccataat tcctcctgtt agttataaaa aatttggctt cccaaaccaa
    20401 tagcaggcag tctatgcgga tgaagataga ttccatttgc aatttgtcct tcttctggac
    20461 aagcatcaaa aatcaatgat acgtgagcta ctgtctctaa attcccattg gccatcggac
    20521 cgacataatc gatcgtatat acctgatcat caaaagcaat tgttccgcct gctttcaaag
    20581 aaaaaggttc gtcttttgat acttcttgaa tcaccgaata ttttcgtaag gcagctgttg
    20641 ctgttttatc gaacaaaatc agcatgggtt cttccttgtt tattgcttct gtcccaattt
    20701 ctgtaatctt tgcctgaatc attgcactct ctcctttaat tgctttcaaa aacagtataa
    20761 cacattctca ttctttagcc gtgtggaata attatgtgat agaataaaaa cgaaaactat
    20821 ttcattacaa ttaggaggaa tcagctcata tgatcgaaaa gataatttta ggaacctata
    20881 cgcgtcgcga aagtaaagga atctatacga tcgaccttga tacagaaaaa gaagaattag
    20941 ccaacctttc attagctgtt gaagaaaata gccctaccta tgtagcaaaa agtaaagctg
    21001 gcaatcttta cacagttact agtgtggatg gattaggtgg tgccggtgcg tacgataagg
    21061 attttcattt tttgaatgcg gtaacagagt ctggcgctcc tttgtgttat gtagcggttg
    21121 atgaagcacg tcaattacta tacggggcga actatcataa aggtgaagta aatgtctacc
    21181 atatattagg agatggcgaa ctgaaagcag cagattcaat attccatcaa gaagcgacag
    21241 gtcctcataa aaaccaagat catgcgcatg tccactatac ggatctgact cctgaccaac
    21301 gattagtcgt atgtgatctt ggtacagatc gtgtgtatac ttatgatgtg tccgaaaacg
    21361 gcaaattgaa tcttgtaact accttcactg cagagccagg aacaggccct cgccatcttg
    21421 tctttcatcc aaatggacaa ttcgcttatt tatttggtga attagacagc acagtccgcg
    21481 tgctttctta taataaagaa acaggtgctt ttgaagaatt acagaaaata tcgaccttgc
    21541 cagaagaatt cgacggtgaa aatggagggg cagctatccg catttcaaat gatggcaagt
    21601 tcctttatgc ttctaaccga ggacataact cgattgctgt attcgcagtt tttgaagatg
    21661 gttcccacat tcaaaacaag caaatcatct caacagaagg ggattttccc cgcgatttcg
    21721 cactagatcc aagcaataat tttgtcgtgt gtgccaatca gaacaccgat aatctgacat
    21781 tatatcgccg tcatcctgaa acaggattac tggaattact gaaaaaagat gtctatgtac
    21841 cagaatgtgt ctgtgtttat tttgatcgtt agtaagaaaa ggagaatgaa atgaatcagc
    21901 aagacgtatt agacagacta cgggaagaac taaatattcc tttctttgat gggaaaatcg
    21961 aagataaaga gtatagcgaa gaggaatatc agaaactcaa agccgattta cagaactact
    22021 ttgaacaata tgtacagaat gtagaaaact aatcagcttt ccatactatc tgaaaagaac
    22081 atcaggacaa gacttttccg tctatcctga tgttcttttc tacaaataaa aaaccgccga
    22141 gatggttaga ctcgacggaa aaggagtatt tactcgattt tgagtaagta caaggttgtt
    22201 gttggttata ggtatgtcat tatcataaag gattttgatc actctatcat aaaggtaata
    22261 ttacatttat attagataac tctagttttt attgttatca aagatttctt ttattttttc
    22321 aacaacaaat tagtcatttt ttggatggca gctgtcaatt ggtctgtcgc ttggtcttgt
    22381 tttgcttcag atgtattgaa tagatcttca tctacccctg attcaagatc gatactttgg
    22441 atcatttctt cacagcctgc tacatatgaa agataagctt tttcaaaatt tttatgaatg
    22501 cccattgctt ttgctggtgg acgcaaagta ctgactttct taagcattgc ccgatatttt
    22561 tttgttcctt cttcaaataa caggatgatc tcttcaagag tcgatttgtc cagttcactt
    22621 gtttttcctt catcaattgc gtttctgatc acttcatatt tcggatgcat tccttctccg
    22681 attgcttctg tttccttgac gatttgatcg atggactgca cataatagcc aatattcggt
    22741 aacatgttcg ttttcctact ttctccattg ttgttattat ttattctact tgtttttatc
    22801 ctcaaatgac aagtacttga aaaaatttaa tggtttctat acagctcgcc attcttattc
    22861 gtgctcaaag aaaccatctg ctaaactata ttgtttccct gcgacaatgt catattggat
    22921 caattgatat tcgtcgtaaa tctcttgttg cgagcgattg aattgcgctt ctttatacca
    22981 ctgtccgttt tgatagtaca gttctcgttc aaacgccgga agttcctgtt ctaatgataa
    23041 aagcagctga taaaagccat ttgttgtctg attcgtctgt tccattagat tcgctgcaaa
    23101 ataaaacgga ctggtgattg catcgtgagt cgtttgcttt tctaattctc ctttactgtc
    23161 gaacataaga aattgtgttt catgcaatgt ttgtttgtca ttttttgctt ggatcgtatc
    23221 tgaatagatc cctggaagat gatcccccca aaaaacaact aaagtccttc tagaaagatt
    23281 cttcaattct tccgtgaact gcttcagtgc cgtgctgcta taggaaatat cttgcagata
    23341 attctctaag tcaagtgtcc cacttccatc tgaagttttt gcaggatagt ctagattatc
    23401 atactttcca tcgtagggca tatgtgtctg catcgtgact aaatgaatga actgaggtgt
    23461 tttttcttcc ttgagaagtg tcattacttc tttgtaagct gactcatcag aaatatatgg
    23521 attattctct atcgtatccg tataggtcat cgtacgctcg ctgatgaact ggtcaaatcc
    23581 taatgtctga taaacatcct ctcttttata catcgaagta ttgtatggat ggatcgctgt
    23641 cgtctggtat gattgtgcgt ttaacgctga aacgatagaa ggaagctgat ccatttttgg
    23701 tacaagcatc gtgtaagggg tggtgagttg cgcattaaat aacgccatag aaaaaccagt
    23761 caatgcttca aattcgatat ttgctgttcc accgccatag ttttgagaaa gcatattgcc
    23821 actatatgtt tgatcagcta cttcataata atctgccaat ggctcaccag atacttctac
    23881 gccgtttaat cgactaggat ctgagaaact ttcactcatc acgaacacga tgttcggcga
    23941 ctcttcctct acagttttat ttttttcatc ggctagcttc tggtattttt cagtgatttc
    24001 tttgatcttt tctttcgaat atccttcagg ttcatccatt ggttctacct tcagattgta
    24061 caaaaaccca ccgataaaac cagtgttata gtagttcatt ttctggctgt aaggtatcca
    24121 tagtgccgtt ttattatatg cttttcgcaa caaattatcg ggattgttga aattactgat
    24181 ataccctagc agtccaattg ccacaagcag tgtcgttaat cggatcaatt ggaatcgcct
    24241 tccttttttc aggcttcgaa acatgtacca gcaaaacaat cctatcacgg ttcctgcggc
    24301 agctaagatc actgtaaaca gcatagtgcc ggtcatatct ttcagcaatc cgatttcagt
    24361 gatcatcttc aaatcgtctg gataaatcgg ttcttgccga taactcattt tcatataatt
    24421 tgcaaaacct aatacaccga tggctacgac atacgtaagg gaacctaaag ggacagatcc
    24481 tgctagtgca atcagaaaaa taagaataat cagcaagacg agacaggcta agaagaattt
    24541 ttccgtatgc caagaaaaag caaatttcaa cgcaaggtct acggatagat cattttgaca
    24601 ccactgcaaa aataaattac taaaaacaac aagaaaaaag atactgatga ttgctgctgt
    24661 tccttttaaa atctttttct gtttggtcga aagtctgctt atatatcttt tcaaatgctt
    24721 gctccttcat ttctatcaat aaaagaatgt tgcttctctt attttagttg tacgaaattc
    24781 gaaagtaaag aacggaaaga agaattaatg aaatccaaat attctctttc tttatggcct
    24841 ttctggtttc tttaataagc ggacctttcg tactccagaa attttttcta gctgttgttt
    24901 cacttgattg agctgttcaa aatcagtact gtctatgtca atcaatatgt agacatgctc
    24961 attcttatgg ttacgcgata tatctgcgat cgctagctcg ttctcattca gcagagaaaa
    25021 tatctctgct aatatattgg ttttcttttg ataaaatata gtgaatcgaa acggcgcttg
    25081 gaaaaacatt cgtgcggatg gataattgac cgcttctcta accgtaccga acaaaagata
    25141 atcttttaag gcttcaactg ccaaacgtcc gctgtctgcc aaagcttcct gagtcgatcc
    25201 ccctagatga ggcaacataa gtatacggtc attttctaag aactcttctt ctggaaaatc
    25261 agtcaagtat ttagccagat gttgtttttc caatgcttgc ataacagcat atttatcaac
    25321 aatttcactt cttcctacat tgataagtac agcatctttt ttcatttttt ctatattcgc
    25381 ctgatcgatc aatccttttg tatcttctgt caacggcagc atgactacga tgtaatccga
    25441 ggtagaaaga actgtttcta aatctgcttg ccgaacataa tccaactggg catcgcggat
    25501 agaataaccc aagacatcca tccctaagct atagcaactt agcgctactt ttgtgcctat
    25561 cgctccaaga ccaagaagac cgatggtttt gccttgcaat tctcgtccca cataagcact
    25621 tcttttgttt tctgcctgtt ctaaaatatt aggacctgtt agtgtttgta ccatacgact
    25681 tgcttcgatt atcggacggg aagataagag caaacagcac aaaacaagct cttttactgc
    25741 attagcattt acacctggtg tattcatgac gatcgtcccg ttttcagaag ctttttcaac
    25801 gttgatcgtg ttcaccccaa caccagctct tgaaattgct agcaaatctg gtgtaagcca
    25861 ttcatctttc actttcgtac tacgaataac tattccttcg gctggcaaat catctattat
    25921 tttgaaatat tccccttgta acttttctat ctcttgttct ggaatagcat caatcaaatg
    25981 aacgtgatac ataaaacccc ctccttacta acaagtatct cgaaatttct gcaattagac
    26041 aactgtttac catttgactt cctagaagca aaaagagcag ttagagcaga gtaggctgag
    26101 tgataaaaat aaggctatga caagttactg ccataagcct tattttcgag agcaaaatat
    26161 tgagggtgag atatcttgat aaatggtgtt ccgttagctg acattgataa taagactaaa
    26221 tttcaaaaaa gttgaaaagc aaatttcgtc aatctcttct tatttcttaa aactaagtgg
    26281 ctttttcaca acctcttttt atctaataac cactgtttcc aactcaacta attttgccca
    26341 cgtttcaatt tgatctgttg ttagatttag cgagacaacg gtatgatgac cgccacctgc
    26401 ctgaatccat gaatggacac cttcatggaa attcggttta gttttccata acacacgtgc
    26461 aacaggtaaa ttaggtgctt cagtcgttgg ttcaaaggct tctacttcat tgatcagcag
    26521 tttataatgc gtaccgaaat ctgccatcga tacgactacc ccttctcctg ctttcccatc
    26581 aaatacaaga cgcgccggat cttctctatc tcccattgat aaaggagaaa caacgattct
    26641 tggtttcgtc gcagcaagcg tcggatctac ttccatcata tgggattcca agattgcttc
    26701 ttggccacta gctagctcgt aagtataatc ttccatgaat cctgtatctt tccctcgagc
    26761 catgattttc agcaaccgat caatcgctgc agttttccag tcgccttccc cagcaaaacc
    26821 gtatccttct gccatcaaac gttgtacagc tagtccaggt agttgtttca ttccatacag
    26881 gtcttcaaaa ttagtcgtaa aagcgttata gcctccttct tctaagaaac ggcgaatacc
    26941 gatttcctgc tgagcttgta cttttacatg ttcctcccat gtcttttctt cataatcacc
    27001 atagtcgaag gtatacaatt ccttgtactc ttcaaataat tgatcgactt cttcgtctga
    27061 tacgcgatcg attacttgta caagatctcc gataccaaaa taatcaacag tccaaccaaa
    27121 ttgaatctgt gcttccactt tgtctccttc tgtgacaccc acgtttctca tgttgtcacc
    27181 aaaacgagcc acttttattc caaaactttc attataggct actgcaacat ccatccaatc
    27241 agcaatctct ttttggatct caggtcgttt ccagtagcca acgacgattt tattttgttt
    27301 atttaatcga gcattgatga acccatattc gcggtcacca tgagcggatt gatttaaatt
    27361 catgaaatcc atatcgatcg ttttccaagg aatgctttcg ttatattgcg tagctaaatg
    27421 aagcaatggt ttttgtagaa gctttgtccc gcggatccac atttttgctg gtgagaaagt
    27481 atgcatccaa gtgatcacac ctgctacttc ttctcgataa ttgacctctt tcatgatttt
    27541 tgtgatcgta tcagcagtaa ctgctaattc ttgaaggcgg attggataag gcaattggcc
    27601 attttcattc agttcatcga ccatttcttg tgcatgtttc tttacttctc gcagtgcttc
    27661 ttccccatat aaatgctgtg atcctacaac aaaccaaaat tctttttctc caatattcaa
    27721 cataattagc cactcctttt atttgttttg tccgtaatac gcattttttc cgtgttttcg
    27781 taaataatgt ttatctaaaa tccgttgagg cagctcttct gaaaatgaat tgagctgtct
    27841 ggtaaataaa ttcatcttgc atacttcatc tagtacgact gcgttcatta ccgcactttg
    27901 cgcatctttc ccccaagtaa atggaccgtg tccgtgtaaa agtacccctg gaattgctaa
    27961 gggatcaatt ttgcgttctt tgaatgtttc tatgataact tttcctgttt catattcgta
    28021 cccgctgtcg atttcctgtt gtgtcaaaaa tcgagcacaa ggtaccgatc cataaaaagt
    28081 atctgcatgc gtcgtcccca tcgcaggaac atccaatcct gcttgtgccc agatcgtcgc
    28141 ccatgtcgag tgagtatgga cgattcctcc gatttgagga aatgaacgat ataaaacagc
    28201 atgagtaggc gtatccgaag agggattgag tttaccttca atgatatgat tgtccaagtc
    28261 cacaacgacc atgtcgtctg ctgtcatctc ttcataactg actccgctag gtttgataac
    28321 gaaatatcgt gtttcaggat caaaagcgct cacatttccc catgtatatt tgacgagtcc
    28381 gtgtttaggc aagtcaagat ttgcttggta tacttcttct ttcagttgtt ccagcatttt
    28441 ctatcactcc tttttcttgc aattgatgaa tcgctgtttt ttcaatgtcg atcccctcac
    28501 ggtaacgtct cataaatatc tgatagcctg ctatttcttc tgctgaaggt gtgatggtga
    28561 ttccttggtc ttgtccaaac acgtcttctg ccaagaagtt ttctaaagac ttcccatgtc
    28621 gatccatcat ataggctgct aataacgcga ttccccacgc tccaccttct ccggctgtgt
    28681 ccatcactgt aactggtgcc tccatagcag atgctagaat ccgctgtcca acttctggtg
    28741 ttttaaaaat ccccccatgc cccactaaac gatcaatttg gacattctca gatttcaata
    28801 tatccattcc gattctcatt gcaccaaaag cactggctaa atggatccgc atgaaattcg
    28861 ctaaatcaaa acggctacct ggtgtacgta caaataatgg ccttccttca tttactcctg
    28921 taatgttttc accggaaaaa taaccataag aaagtaatcc cccgcaatcc gtatcgcctt
    28981 ctagcgcttt gttgaataat accgtgaata atttatctct gcttatctcc atacccaaag
    29041 aattggtaaa ttcttcaaaa atactgaccc aagcattgat atccgaagag cagttattcg
    29101 tatgaaccat gcctactagt tctcctgaag gtgtagtgac catgtcgatt tccggatgga
    29161 cattttctag ttctttttct aatacgatca tagcgaaggc ggaagtacca gccgaaatgt
    29221 tccctgtccg tttggctaca ctgttcgtag cgaccatacc ggttcctgca tccccttctg
    29281 gcggacaaac cgggattcct ggtctcaaat ttcccgtagg gtcgatcaga taagcccctg
    29341 attcgcttaa cttgccagct tcttctcctg ctactagaac tttaggcaaa atgtgatcca
    29401 agtcaatcgc tacgtgcgct ttttcgatta actgaccaaa aatatttttc atttcttgat
    29461 ggtaggtttt cgttgaagga tcgataggaa acatgcctga tgcatctcct acgcctagta
    29521 ctttctcacc agtaagctgc caatggacat aacctgcaag cgttgtcaaa aaattaattt
    29581 ctttcacatg ttcttcttta ttcaaaatag attgatacaa atgagcgata ctccatctct
    29641 gcggaatatt gtattgaaat ttttccgtta atattcgttc tgcttcttca gtgattgtat
    29701 ttcgccaagt acgaaatggt actagtaact gttccttgct atcaaatgct agataaccgt
    29761 gcatcattgc actgaaccca atcgacccga ttgtcgtaag attctcgcca tagatttcga
    29821 atacttctgc tgccattttt cggtaactga cgcgcagtcc tttccaaata tcttctaatg
    29881 aataggtcca gataccattt tctagtttgt tttcccactc ataactacca gcggcaatag
    29941 gagtaaaaga tgaatcaatc aatacagctt tgatcctagt tgatccaaac tcaatcccta
    30001 aagatgtacg accatctatg acatctaaca tactttgcgc atttacttcc ttcataatga
    30061 tttaactccc tccttctcgt caaacctgat caaaccgtct ttttagtagc gcttacaatg
    30121 attattgtat acttttgtac gtacatgtca agcggttatt tacaagaata caataaaaaa
    30181 cacttgtttt gtacgtacat aagttataaa atgaaattgc ttacaaaatc tgatcaaccg
    30241 ccttctcgaa attctagaag gagagaatac ttatgaaaaa agaaaaaaaa gtttcctcat
    30301 cgtttattta tttttttggc gcatttggcg gcattctatt tggatatgat attggtgtta
    30361 tgaccggcgc cttacctttt ttacagcatg attggggttt ggcaggtaaa gcttctttga
    30421 taggatggat cacgtcttcg gtcatgttag gtgcgatcct tggcggctct ttatctggtc
    30481 ttctttctga taagcttgga cgacgaaaaa tgattcttct ttctgcactt atttttatgg
    30541 ctggttctgt cctttctgca tctgcccctc acaatggttc ttattttctg attgcagcaa
    30601 ggattcttct aggtttagct gttggcgctg catctgccct agttcctgct tatatgtctg
    30661 aaatggctcc tgctagacta aggggaagat tgtcagggat caatcaagtg atgattgctt
    30721 ctggcatgct cctttcctat gttgcagatt atttgctaaa agtgttgcct gaaacgatgg
    30781 cttggcgcgt gatgttaggg ctcgctgctg tccccgcgct gattttattt ttcggtgttt
    30841 tagctctacc agagtcaccg cgttttctta tgcaatctgg ccgcttggaa gaggcaaaaa
    30901 gagtattgaa ctatattcgg acaccaaaag aagctgaaca ggaattcgaa caaatccagc
    30961 tgaatgtcaa acaagaaaag acaaccggaa cttcttggca tactttgttt ttagaaaaat
    31021 atcgttcctt agtcattgcc ggtattggtg tagcagtatt ccagcaattt caaggagcta
    31081 atgctatttt ttactatatt ccgctgatcg tagaaaaagc gactgggcat gcagcgagcg
    31141 atgcattgat gtggccgatc atccaaggaa tcattttagt tgctggttct ttgctctttt
    31201 tggttattgc agacaagttc aatcggcgaa ctttattgaa aataggcggt agcgtgatgg
    31261 gactttcatt catcctacca gctgttttag gaactgtact ggatgctcat acaaattcgt
    31321 tgttgattct cttgttttta tgtatttatg tcgcatttta ttcttgcaca tgggctccac
    31381 tgacatgggt aatcataggc gaaatattcc cactagctgt ccgcgggcga gcatctggcc
    31441 tagcctcttc ttttaattgg attggttctt tcctagttgg cttgcttttt cctgtgatga
    31501 ctgcttctat gtctcaagaa atcgttttcg gcatttttgg gatcatctgc tttttagggg
    31561 ttttatttat tcaagagatc gtaccagaaa ctcgtgggaa atctttagag gaaatcgaac
    31621 aaagtgcatc taaaaaaact tatcctaaac gtatttctat ccatcattct taaagaggtt
    31681 gtgacagaag tggtcagctt caagaaataa gaaggaaccg cttagtttcc agtcacaagg
    31741 aacaatttca aaaaacagct tctcatgttt gatgaatatt gtgacttgtg accagttggt
    31801 aggagaacac cgtttagtgg agaggtggtg aaagaaacgt tcagccttga gcgatgacaa
    31861 aagctctcac aaacattgcg acgagtaacc gtaggagcaa taagaggcgg taaaaacccg
    31921 tctcttattt ttcgttttct ttcactgttt cccgttcaac tagtacaggt tcatagagaa
    31981 tatttggctg tgcctgtcct ttttcaatcg ttgtgacgat ccaccgtgcg gcatcttgtc
    32041 ccaacttttc ttttggatgc gtcaatgttg tcaatttttt attccctaaa tggcttaaag
    32101 aagaatcgtc tgttcctacg atcgataggt catctggaat atgatagcca ttttctgtca
    32161 acttatctag cattaaacta gctatttgat cgttatagca aatgatgcca tctaaagctt
    32221 gttcttcttt caaccgttgt actgtttttt cagcgattgt ttctcgtgtt tccgtggtga
    32281 aagtaatgat atcttccgtc aaaaaacgca cattatactg ttcacacgcg ctgataaaac
    32341 ctttcatcct gtattttcct tgaagatcgt cgatttttgt gatgaacaac agacgctgat
    32401 gtcctttttt tatcaaatat tctgctgcca taaaaccgac tttcacatca tctaaacaaa
    32461 tataagaggc atttaactct tcataagcag cattgatcat caccatcgga atgccttgtt
    32521 ctctcaaagc aacataaagt gctaagtttg ggttatactg attacttttc gtaggctcaa
    32581 cgatcaaacc atctacccca taagcaatca ttttcttcaa acactctttc tccaattcgt
    32641 gatcgttatt cgtactggct aaaagcaatg aatacccaga ttctcgtaat tcattttcga
    32701 ttcctcggat aatcgacgga aaaatataat cagagagata tgtggtgatg acaccgatcg
    32761 tttttgttct gttcaatggt gttttttctt tcttatagct gtcatctaca tatgtgcccg
    32821 accctttttc tttacgcaga tagccctcat ttaccaacac agcgattgct tgccgaatcg
    32881 tatgtctgct gacgtcatat atttcttgca gcttgaattc tgagggaatg gttgtattta
    32941 cttcgtattc ctcacgaagg atcttttgtt ttatctcttc tgcaatatct tgatattttg
    33001 ccatttgcct tgcccctttt tatttgtacg tacatatatc ataacgagtc tgcaaggttt
    33061 attcaagcaa aaatcaaggt ttcttgtgct cataaggcat acggaacagg ttcaccaaat
    33121 tctggaaatc cttgctcatt ccacgtaaag cattgcgcgt tcgcgtgccg attaggattg
    33181 tctaacggat cccctttttg gttcttctct ggtcgagcat gatagatcaa aatatcaaag
    33241 cacccgtctt ctgaaaccgt aaacgagttg tgaccaggac caaattgctt atttttttca
    33301 gaagaaacga atactggctc cgataattta tgccatgatt cttttaataa aagattcttg
    33361 tgctcatcag cccacagcag gcccatggca tagttttcat ctgttgcact accagaatac
    33421 gtaatgaata ttttcccatt gcgagtaagg actgctggac cttcatttac tgaaaaaccc
    33481 cgtttttccc attcatactc aggtatagaa aggaggatct gcttcccctt taacgtccaa
    33541 ggattatcca tttcggatag ataaagatta gaattcccag taatttcagg atcttgttgg
    33601 gcccacacat aatatagctt tccttgagaa gagaaagttg ttccgtctaa actgaaagtt
    33661 tcccttttcg tttttatttg tcccttttct atccaatctg tattcattgg atctatatgc
    33721 tgattttcaa gtacgaacat gcggtgatga tgagattcat cacgtatagt tttatgattg
    33781 cttgcagcaa agtaaatata ccattttcca ttgatataat ggacttcagg tgcccagatc
    33841 aattcactca tcggtccttc ttcatgagcc tgccatacaa tcaggcttgc agcatgttct
    33901 aattcattca gtgactttgc ccttcttaat tcaatcactt ggtagcctgg aaccgatcct
    33961 gtaaaataat aataaccatc tgtatgttta tatacccaag gatctgcccg ttgccgaacg
    34021 ataggattat tataaagtgt cttcatcata acaaatttcc tcacattttc tttattctaa
    34081 ttacattcca agagtatttt tctatcccta catttaattc gtcatgtttt tctattgaaa
    34141 aatagtttat ttcaggtttt attttttctg gttcttcacg tgtatttttg tccaacagat
    34201 cgctactggt taatgtgtag tgctctcctt gatctagcaa attgaaatca tgttcaatcg
    34261 ttatcttttg ttgttcattt gtgcgattga tcaagaaaat agtcagctga ttgccgttat
    34321 ctacgatcac actgtccaca taaggaacat tttttatctt ttcacaatca taaacagggc
    34381 aaatgatttg ttgtttcaat acttgacctt tagcatattt cgataagtgt tgcatcacat
    34441 aataagttgg attcctccat gcttgtcctt tagcatctgt cagtattaaa ggtattgtgt
    34501 ttactaacaa tgattgacaa ctaatcttta cgcgatctgc gtgtcgtaaa atagccagcc
    34561 ctaatgaacc aaataataat gtatcttcca tagtaaagta acaccaatcg atgggactac
    34621 cgacttcaaa atccctatgt cttttttccg gttgggcatg tacattccat tcatcaaacg
    34681 ctagatacat tgttttctca cttctcaaat atccttttac ataatcacaa gtagcaacga
    34741 tttcttcaat ttgacgatcg aatcgataag cttttgcaag gtatgtcgct gtatcatcac
    34801 gctcattttt aggaggttta gtcaagtctt cttcatcata cttatcaata tagttatgca
    34861 aagctaaata atctacattc tcataagctt cttctaatgc gatacgatcc cattcagggt
    34921 acgatggtaa acgtggcgta gagcttccaa ctaagattgt ttcaattgaa tcatccacta
    34981 acttcatgac ttttgaggct tcatttgcta acctaccata ttcataagca ctcttagccc
    35041 ccacttgcca tggaccgtct agctcattcc ctaaacacca taatttaacg ccaaaaggct
    35101 ttttttgccc attttttatt cttaaatcac tccagaaggt tccttttgga aaattacaat
    35161 attctactaa attttttgct tcatcaattc cttttgttcc taaattcacc gtgaatatcg
    35221 gtttcgcttg tacatgactg atccatttaa aaaattcgtg gagtccaaaa gaatttggtt
    35281 ctatacctcg ccatgctaaa tcaatttttt ttggtcgttg ttctttccta cctacagtat
    35341 ccacccaatc ataaccagat gtaaagtttc ctccaggata gcgaatgaca cctaattcta
    35401 attcctttac ataagagagg acatcttgtc taaacccgtc accatcagct gtttcatggc
    35461 ccggttcata aatccctgta tagacgacac ttcccatatg ctcaacaaat gagccaaaca
    35521 gccgagaact gatctctcct accgtaaaat cctctctgat ttttactttc acttcttcgt
    35581 ccaacagctt tccctacctt tcatttccta tagtcaagat aatagataca agtttccata
    35641 cgccaccagc aaagataaaa ataaaaggag ggaaaagaaa tatgagcagt acaaatccta
    35701 ataacaatac gcaacaaaga atatttctca aaaaatcaaa aaatactttc catgcacttt
    35761 gttcaaaaat ttgtaaaaat gtgatatctt tgttttcaga aatcacatat atccaggata
    35821 aaagaaacat ggaataaata agtaaaacta catatccact aattgaaagt acgattgtct
    35881 gttcttctaa taattgatta acaagaaaat aaataatgat cgttaacaga ctaaggaaca
    35941 agttcttacc aaaatgttgc tttattcctt gaaagaaaaa ataaatgata tttcctgatc
    36001 cctcaccttt ccattgcgcc attacataaa aaacagcaca tgtagcaggc acgatagtaa
    36061 taacaggcaa acacacgatt acccagagaa tacctaataa gatgtaatca gaaaccacaa
    36121 tcaactgtcc ataaaattta gtctcaacta ttttcatctt cctcactctt tcattccaga
    36181 catagctgtt ccttgaacaa aatatttctg tgcaaaaata taaaccacta gtaatggcag
    36241 cattgaaata accgttccag ccattacagg tccataaaaa gaaatgttct gccctgtaaa
    36301 taaagctaac cctgctggta aagttctcat ttttgaacta cttgtcaata tcaatggata
    36361 aagcaagtca ttccaactat tcatcagcgt gaaaatccct aatgataaaa gagtaggttt
    36421 gctcaatggc aacatgattt tcaaaaaaat attaaactca cttaatccat caatgcgtgc
    36481 tgcctcttcc aaagaatctg gtaaagtaat aaaaaaggaa cgcatcatga agataccaaa
    36541 tgcagttgtc atacgaggaa tgatcaaacc tgcataagta tcaagcatgc caagcaaatt
    36601 gacttcaata aatagaggaa tcatgaagac ttggaatggg atcatcattg tcagcaaaac
    36661 gaaatagaac aaaactgatt ttcctttgaa acgcatacga gcaaatgcat accccgcaag
    36721 agaatcaaag aatacagaag tgatcactgt ccctccagca aaaatcactg tatttttgat
    36781 gtaatctaat aaagggatcg ttgtccaaac cttaatatag ttatctaggg tatagctatc
    36841 tgcaaatatt ctaggcggaa atgagataat gtctttttcg tgtttaaaag aagaaataat
    36901 cagccacaaa aaaggaaacg caataaccaa caatataact agcatactaa tcgttcgaat
    36961 gattttttta ccactaactg cttttcgact attcacagta actgtaatcg agctttctgg
    37021 tgccatacag gtttccctcc tatttcattt gtttttcttt ccgtgacatg tacacattag
    37081 tggagatagc tatagttgca acaatgataa acaaaaccga agacagtgct gatgcatagc
    37141 ccaattcata aggtgctgta aatcctcgac tgtaaatata ttgaacagct gtttctgtct
    37201 taaattgtgg gccaccagcg gttgcaacat atacttggtc aaacacttgc atcgaaccaa
    37261 tcagattcgt taaaatacaa aaacctaagg agggaacgat ctgaggtagc gtaatgttaa
    37321 aaaactgctg tcgatttgtt gcaccatcca tttgagcaga ttcatacaaa gagggagaga
    37381 ttccctgaat agcagtcaag agaatcgtca tagtaacacc aaaattcttc caaacagtca
    37441 tgatggctac cgtcggcata gcaagggttg gatcgcgaaa aaattgaggg atcggcaagc
    37501 cgacttgtga aaacaagtaa ggaatcgccc ctacattcgg atcaagcaac atagaaaaaa
    37561 taatcgcaat tgacgtcaat gaacaaataa ctggaatgta aaatgtcgaa cgacaaaaac
    37621 gattgaatct ggtattttta gataatgcta ctgctgctaa taaaccaacg ataatttgtg
    37681 ttggcgtttc gagtacggtg aaatataaag aatgaatcaa cgaattaact gctcgttcat
    37741 cactaaatat acgtgtgaaa ttttcaaaac ttacaaaatt tcttgatgta aagaaaatat
    37801 tcaaatctgt aaaactaata gcaaatgttc ctaaaagcgg tatgacagta aataataata
    37861 aaactaaaaa agatggagtt aaaaacaaat acgcagcacg atcaggacga ttccaatatt
    37921 tggtgaatcc gttcatatag tccacctcat ctttcatttt gataaaatat acaatcttgg
    37981 tttaaacgaa tctgaataag atatccgaac aatcgctgtg atttttgcaa taacacctat
    38041 ctgcaaatac taggtcgatc attcagatat ctgtatcttg ccaacagtct atcctgtata
    38101 aagttctgta ctatttttgc agtaattgat tgatggcatc atctgcatcg ttcatgagtg
    38161 tctcaggatc ttctcctgct aataattttt caatcagtgg attgatgata tcgttattga
    38221 tcgcagagat ttgggtaaaa cctggtaaat atggctctgc atagtccatt tgtttcgata
    38281 attcagatac aataggatca ttttttactt cttcatcttt tgctactgaa tccaagtagg
    38341 caggaaaacc attttcgata ctccattttt taccaatttc agttgtattc caatatttca
    38401 aaaactcata gacagcttct tttttattat tatctgtact tgccggaatg gcatatccta
    38461 cgccatctaa aatagctgtt tttttgtctg aatcactccc ttgtggtaag gtagtcacat
    38521 tataatcaat ctcgtttgct ttcaatccgt tatttaacca tggcccattg atttcaatgg
    38581 ctagttgacc agcattcatt aaattgtcca tttcggcgcc agaaatcgat tctggaccag
    38641 tcttgtctac atggatcatt ttttggatcg cttttaacgt tttaaggttc tcaggagacg
    38701 caaattcaga cttagtataa tcatcgttca ctaatttacc gccatttgct aacatccagt
    38761 tatataacgg tgcagttcca tccttattga agacaaaacc tgatacattt ttactactat
    38821 cagttaattt tggtgccatt tctgtcaatt cttcccaagt agtaggtggt tgttcagtat
    38881 ctaatccagc ttgtttaaac aaatcattat tccaatacat atacatgcct tgtacctgca
    38941 tggggacaaa atactgttta ccttctactt cacctaattc aatagccgtt tctttaaaat
    39001 cagatttatc tactccatca tattcccaga aatcgtctaa tggttgtacc gctccgtttt
    39061 ctacatactg tgcaaaatct ggataattta atgcaataaa gtcaggtgct tttttagaag
    39121 aaatcgctgt aggaagtttt tcattcaaat tggcccaagt catgatatcc atttcaattt
    39181 tgatgttttt atcatttgtt ttattaaatt cattaacgat ttcttgcaat atttcaccat
    39241 cagatgcagt aaacccattc caaaaagtca aagtcacttc tttatcagaa tcagattctg
    39301 ctgaacttcc gccaccgcaa gctcctaaaa tacctgttgc agctaccaac aaagagccaa
    39361 ttaaccattt tttacctttc attttttgtt cctccttcat ctgataaacg acatctgacc
    39421 acattccaag atagcggttt caataaacaa ttgattttcc catcttcagc ttttacattt
    39481 gttgaatgac ttggttttac ttgttcactt ttaggaagat tagttgcttt tgtatcaaat
    39541 ccactcattt cagaaaaatc aatcactccc tctaatgata attcagacaa gtgaacagtt
    39601 agattcattt cttcttctcc acggttaact gcaaatatga caagttcttt gtcatttatt
    39661 acggctatac tttctaaata cggcacttct tcaaaatctt ccgtcgcata gtttgctgaa
    39721 cgaatttgtg ggcttaacac ctctccttgt ccatacacag cagcttgcat aaatggataa
    39781 tagatcgtct gtctccaagc ttctccgttt gtttctgtcc tgattggtgc aataacgttt
    39841 accaattgag caagacaagc aatctttact ctatctgcat gtttcaataa agtgattaat
    39901 agacagccta atagtaatgc atcttcaaaa ttatagttat cttctagtaa aggaggagca
    39961 atctgccaag gcgctgcttg ctcatcttgt tccgctgaat gataccaaat attccattca
    40021 tcaaaggaca ggttgatctg cttcttacta tgtttcttcg ccttgactgt atcgcacatc
    40081 gctgtcactt cagaaatgaa ccgatccatg tctaaggaac gtgcaagata atgagaaaga
    40141 tcattttttt ggttgccata atattgatgc agagacaaat aatctatttg atcgtacgct
    40201 tgatcaagaa ctgttaactc ccaatcacca aatgtaggca tttgccgatt cgaacttccg
    40261 cataaaacca cctcaattga atcatctacg agtttcatcg ccttagccgt ttcctctgct
    40321 aaccgtccat attcttccgc tgttttatgt ccaatctgcc aaggaccgtc catttcattc
    40381 cctaaacacc atgtcttaat tccaaaaggg ctctcttgac cattcgaacg ccttaaatca
    40441 ctccaataag ttcctttagg aaaattacaa tattcgacta aattacgagc tgcatctact
    40501 ccacgagtac ccagattgac agccatattg atttcggccc ccacatgctg acaccagtta
    40561 taaaattcat gaatcccaac ttcattcgtt tcaattgtcc gccaagctaa atccaacctt
    40621 ctaggtctat ttgctatggg cccaatactg tcttcccaac gaaatcctga cacaaaattt
    40681 ccaccaggat atctaatcat gggaatgttc aattctttta ccaagtgaat gacgtcttgg
    40741 cgaaatccac ttttattagc tgatggatga tctggctgat aaatcccttc atatactgct
    40801 cgtcccatat gttcaataaa tgaactgtac aaccgcggat caactttgcc tacacgcttg
    40861 acactactaa tcaacattgt ggcttccata actgacctcc tttaatttat acatgaataa
    40921 tacacctcgt tgaaagcgtt gtcaataata taaatgttaa acttacttat tttttatcga
    40981 ttttaattaa ccaaaatatt aataatgatt aaaacaaata atttaatata acttttatta
    41041 acaaatatgt taatatataa gaaaaacctt ttaagaggtg atatatatgc aactacaaat
    41101 aaacgaagaa tccttaccgg tatatgaagc attagctagt aaaacaagga tcaagatcat
    41161 ccagctactt tccaaaaaaa agatgaatgt caaagatttg gcaaaagaac ttggagtgag
    41221 cagtgctatc acgacgatgc acgtgaaaaa gctagaggaa gcaaacatca tcaaaacaga
    41281 aaaagtagga caacaaaaaa tttcgagcct tcgtgtcgat aaaatcgata ttagttttcc
    41341 agaaaaaata ttcaatgctt ttgatacgaa agaaacatcg atacctattg gtcactatac
    41401 aaattatgca attgaaccaa catgcggttt agctacgatc catgatttta tcgggaaagt
    41461 ggatgaacca cgctatttta tggatcctcg aaggatggat gcccgcattc tctggtttac
    41521 cagcggattt gtcgagtatc aggcacctaa ttttttaaat acagaggata cattggagat
    41581 gttggaagtt tctgtggaaa tttcttctga atttcctttt tcaaatgaca attggccttc
    41641 agatatcact ttcagcctaa atggtgtgga gctaggaaca tggacgagcc ctggagattt
    41701 tgctgatatc cgcggtaaat atacgcctga ttggtatcca gataatctta atcaatacgg
    41761 cttgctgaag acgatccgaa ttaccaaaca cttgacgaat atgaatggag aacccttgtc
    41821 aaacatcacg atcaacgaca tccctaaaga gcaagacact tggcatttgc gaatcgaagt
    41881 gaaagacgat gccaaacatg tggggggctg tactttattt ggtaaaggat tcggtaatta
    41941 cgatcaggat atcaaattaa aagtttacta cagctgatat ttttcttttc tattttataa
    42001 aacaaaaaca gccaatccct atgaagggac ggctgttttt gctttattgt tatttccaga
    42061 aatcatccaa aacggaaatt ggcaaatgtc gtttatgttg tgtcttgttc caccatgctt
    42121 cgattttttc ttgtgcttga gcgggaactt cttttccttc tagataatca tcaatttcat
    42181 tatatgtgac acctagtgcg acttcatctg caatcattgg tttcccatct tcaagatctg
    42241 cagtcggaat ttttgtgtat aatttttccg gtgcattcaa tgcttgaagc aattgtttcc
    42301 cttgtcgttt gtccaaacgg aataatggca aaatgtcggc tccaccatca ccaaatttcg
    42361 tgaagaatcc agtaatgttt tctgcagcat gatctgtccc taatacggct cctgctcttt
    42421 ctccagctac ggcatattgc gtaatcatac gttgacgtgc tttgatattt cctttgttaa
    42481 aatcgctgat tcccacacct gcttcgctta atacttgtac ttgtgcatca acagctggtt
    42541 tgatatttac tcgcagacta acatcgggct ggataaagtc aagagcagct tttgcatctt
    42601 cttcatctgc ttgttcacca taaggaagac gaacagcgat aaattgatag ttttcatctt
    42661 ttgtttcttc acgcatttct tccatcgtta gttgcgccaa acggcctgct aaagtagaat
    42721 cttggccgcc gctgatacct agtacaaaag ttttaagaaa cggatatttt ttcaaatatg
    42781 ctttcataaa gtcaatactt ttacgtattt cttcttttgg atcaattgtt ggtttgacac
    42841 ctaattcatt gataatttct gtctgcaggc tcatttattc tcctcctatg cttctgtatg
    42901 aagttctgct acgctttttt tcattcgttc catgatagcc attttatggt tccagcattc
    42961 agtagacaga tcgactgggt atttttgcgg attcaagtct cgtttatact cttcccatag
    43021 tgaatctaga ttttcttttg tatattcttt gatttcgttt agtgcaggta attcgtaaac
    43081 tctttttcct tcgataaaga tatcttgtaa aactgggcga gctgtaaagt ctcgaacagt
    43141 tttattgata aacgtatgca ctggatggaa catgaagata gcttcttctt ctcgagggtc
    43201 ttcatcccat aatgtcacgt agtctccttc agatttccca tcaaaatttc gagtgatgcg
    43261 ccacacttgt tttttccctg gagtagttac tttttctgca ttgcttgata acttgatcgt
    43321 atccatcatt tttccttcat catcttcgat cgatactagc ttgaatactg caccaagggc
    43381 tggctgatca taggctgtga tcaattttgt cccaacaccc catacatcaa ttttagcttt
    43441 ttgcatcttt aaattcaaaa tcgttgcttc atccaagtca ttcgaagcat agatttttgc
    43501 ttccgtaaat ccggctgcat ctaattgttc ccgaacacgc ttagatatat aggccatatc
    43561 tccactgtcg atgcgtacgc cttggaagtt gattttgtca cctaattctt tcgccacgcg
    43621 aatcgcactt ggcacacctg atttcaacgt atcataagtg tcgactaaga acacgcagtc
    43681 tttatgggtt ttagcgtagg ccataaatgc ttcataatca tttccataag attggactaa
    43741 cgaatgagca tgggtcccgc tagctggtat cccaaatatc ttcccagcac gtacattgct
    43801 tgttgcatct gctccgccga tatatgccgc tctagttccc cagactgctg catccagttc
    43861 ttgtgcccgt ctagttccaa attccaatag tggatcttct ccgatgacag atttgatacg
    43921 agctgctttt gtcgcgatca acgtttggaa attgaccata ttcaacagtg ctgtttctac
    43981 taattggcag tgcgctaaag gcccttccac ttggatcaat ggttcatttg caaaaactag
    44041 ttctccttca cgagcagaac gaactgttgc tttaaattca aaattttgta aatactccaa
    44101 aaaaccttca ggatagactt ctaatccacg caagtaagca atatcggaat cattgaacgt
    44161 cagattctct aaataattga ctaaccgttc caatcctgca aaaaccgcgt atccgtggtt
    44221 aaaaggcatt tctcgaaagt aacattcaaa aacagcatgt aaatcagctc gacctaattc
    44281 ccagtatgtc tgcatcatat tgatttgata tagatccgta tgcagcgtca aactgtcgtc
    44341 tgggtaagta ttattcatct tgtattactc ctcttggcaa gttttcattt cctacccatt
    44401 ataacaaaaa aaattaatag gaaaagaaat cttccatttt ttctgaaaaa gcacatgttt
    44461 tacggatatt tcttgctaaa aatgaactcc atctgctaaa actatccttt cttattggta
    44521 tttaacctaa acgagaaaga atacttattt tattcaatgt atccgagaca cgaagaaatt
    44581 agcgttttct atttttctta cttgataaaa taagtagatt ttcctactac aatcgtagcc
    44641 gttaagatac cgatgattga acaaatataa ccaaatgtct ctacaacata atcaatggca
    44701 aaagtttttg aagcaataat acgtctttcc ttttttgctt cttatcccca aagaaacgga
    44761 tataacttat tatgtattga atttttttgg aaaggcgaat tatgacaaga tataccttgc
    44821 gttgaaaaaa ctcgcggaaa aaatatcaca cgtttttaaa aagatacaaa gtaagaaagc
    44881 agctgtcaat agaatggaaa caaaaatggc gagacatcaa tcagtctcgc cttgtttttt
    44941 attgtataca ttagatatgg agcaggttac cataaagatg attattagtc gttattaaat
    45001 agaatccttc gtattttttt gttctatcac tacaatatct ttatttggac gtttgaattg
    45061 tattccggca cgttctagct cctgtttcac tcctacatgg actttgacat cttctcgtgt
    45121 catttttaaa acgtgatgaa tcgcatagtc gatttcttgc cttgtgattt ttttcttcgt
    45181 attgatcatc acgacttgct ttttatcata tgtgtctacc aatagcagtt gtgttcgatc
    45241 taacgaaaat aatttgtaat aattaatagc cgtattccca aaaaagaatt ccactacttt
    45301 aggataaaat gtcttaatat ttaaattact attcacaggt gtttcgatta gcttcatctc
    45361 attcctcttt tctatatagt cgatttagat ccaatcttaa gttatgttac catctttcct
    45421 agcattaagg aatcggttac aattgaacct ttaaagacaa aaggatttac cttttatcac
    45481 ttaatagtat aacataattt catgagaaaa aaatacaatt ttttcttgac catctcgtta
    45541 cgtaaaggat tatgcttaca tttgtcagga ggaaaaataa tggagtacac aataaagaaa
    45601 atgtcagaga tatcaggagt cagtccgaga accttgcgct tttatgatga gattggactg
    45661 ctaaaaccag ctaggatcaa ttcttcaggt tatcggattt atggcaaaaa agaagtcgat
    45721 cgtctgcaac acatcttatt ttatcgaaca atggcattca agttggatga tatccaagag
    45781 gtcttagaca atccatcttt tgaccaccag aaagctttaa tcaagcatca gcaaatgttg
    45841 ctggaaaaac gggcacaaat cgataccctc ttgacaactg tccaacagac acttgatatg
    45901 tacgaaggag gaagaaaaat gagtgacaaa gaaaaatttg aagggtttaa acaacaaaag
    45961 ttgaaagaaa atgaagaatc ctatggacaa gagattcgac aaaaatacgg aaaaaaaaag
    46021 gttacagcat ccaatgaaaa atggatgaat atggataaag aaacgtatga agaaatgcaa
    46081 actgttgaaa agcagatgtt atttgatctc actctttact taaaagaacc agaagatgaa
    46141 agcttagcag accgtatctt ccaagctcat aagaagtggt tatcttacag ctggcctgaa
    46201 tatcagccaa aagcgcataa aggactagga atgatgtacg aagccgatga acggttcact
    46261 gcatattatg acgagcgaag tggtcatggt gcagcaaaag ctttaaatga aattatccaa
    46321 aaaaaggcgt aatcttttac atgatccaac aaaaaagtac acgtaaaatg acctaataac
    46381 aaaacagcag acacccaagt tagattttta tgctaacttt tggtgtctgc tccattcttt
    46441 cacaagtttt ttcatcctac tttaaatata ttcatcgttg aataaacgta taaggtattt
    46501 tcactttatc ctttttttct gtaagaaaca attgaaatgc catttctccg caagcttcca
    46561 gatggtggtc gatggttggg aaaccaagaa gctcgctgat cagcaaatta tcgcgtccaa
    46621 tcaccactgg tgtcttatct tgcagatagc tttgcaagat gcctgctgca accatatccg
    46681 aatttgtaaa gacaccatca actccaagct ctttaaaaaa gaagcctgct tgttttcctt
    46741 tctctgcatc tatacaatcc caaaaaacaa gtgattcatc aaaagatgga aaaagttgat
    46801 gacatagctg caaagtgatt tttgagttat aacttagcct gccacttcgc cccaacgtga
    46861 tgcccaattg ctttatacct tgctgcttca aataagtaag accttctgta attgattgtt
    46921 ctcgatcaat aaagacacag cttgcctgtt ttcccttgat ttcttcacaa aaaactagag
    46981 gtccatattt ttgatatgcc aataaatcat ctagtgtatt ggcacgagac gtaatgatca
    47041 atccatcgaa agctttagct gcaaattctt caagatacct tcgctctttt tctttctgat
    47101 agcccgtcgg taaaagtgtc actttgtaat cataatcaaa cgctgcttca atgatcccac
    47161 tgatcaactg atcaaaatac ggatgattgg tataaggcag aatcactcca atgcttttcg
    47221 tttttccata acttaactgt ctggcatttg cattagggat ataatcgact tctttcatga
    47281 ccgcaagtat ttctttcctt ttttcctcat ctacatatgg atgtccattg attacacgtg
    47341 aaatagtaga aactgaataa cctgtgagct ttgcgatatc tcgaatgttt gccactgctg
    47401 cttcgccacc tatacaaaaa gattttcttg taaatagtct accacagctg gaccgcattc
    47461 aaaaaactga tacatttttc cttcatgcga aaaactgatc cgctgcccgc cccaatatat
    47521 atcttttgtc aaattttcga tttcattcat tggtatttct tttactatat tcctattcaa
    47581 cccttgaaaa ctgtacaacg ttaatacgga tcccttgaac tcgacaaacg cataaaaata
    47641 attgttcgtt gtgccatctg ttatctctac ataagtattc attgcaagcc ctccttttga
    47701 tactttgagt atagtgtatg aaacgcgtct catagcaagg agtttgtttt gccaaagaaa
    47761 agttttattc ctctttcatt attaccaaag tctcttattt taaattgaat acattataat
    47821 gatactgtaa atatatcttt attattagtg aatggatatt tacttttaat agataaaata
    47881 ttataactat taaaaataac tattttcatt ataattacac acaaaaaaga gcgtgagaca
    47941 aaagtaaaga ac
//
LOCUS       DAJSBL010000019        43732 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.019, whole genome shotgun sequence.
ACCESSION   DAJSBL010000019 DAJSBL010000000
VERSION     DAJSBL010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 43732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43732
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.019"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(372..2753)
                     /locus_tag="ONT85_001229"
     CDS             complement(372..2753)
                     /locus_tag="ONT85_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HCR2378217.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(2743..3390)
                     /gene="recU"
                     /locus_tag="ONT85_001230"
     CDS             complement(2743..3390)
                     /gene="recU"
                     /locus_tag="ONT85_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HCR2378218.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            3454..3993
                     /locus_tag="ONT85_001231"
     CDS             3454..3993
                     /locus_tag="ONT85_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HCR2378219.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            4083..4502
                     /gene="gpsB"
                     /locus_tag="ONT85_001232"
     CDS             4083..4502
                     /gene="gpsB"
                     /locus_tag="ONT85_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HCR2378220.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYEAYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            4524..4891
                     /gene="rnpB"
                     /locus_tag="ONT85_001233"
     ncRNA           4524..4891
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ONT85_001233"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            5100..6266
                     /locus_tag="ONT85_001234"
     CDS             5100..6266
                     /locus_tag="ONT85_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HCR2378221.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            6312..7808
                     /locus_tag="ONT85_001235"
     CDS             6312..7808
                     /locus_tag="ONT85_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HCR2378222.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            7889..8572
                     /locus_tag="ONT85_001236"
     CDS             7889..8572
                     /locus_tag="ONT85_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HCR2378223.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            9217..10407
                     /locus_tag="ONT85_001237"
     CDS             9217..10407
                     /locus_tag="ONT85_001237"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HCR2378224.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            10509..11462
                     /locus_tag="ONT85_001238"
     CDS             10509..11462
                     /locus_tag="ONT85_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HCR2378225.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            11531..12880
                     /locus_tag="ONT85_001239"
     CDS             11531..12880
                     /locus_tag="ONT85_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR2378226.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(13063..13386)
                     /locus_tag="ONT85_001240"
     CDS             complement(13063..13386)
                     /locus_tag="ONT85_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2378227.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            13515..14678
                     /locus_tag="ONT85_001241"
     CDS             13515..14678
                     /locus_tag="ONT85_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HCR2378228.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            14748..16097
                     /locus_tag="ONT85_001242"
     CDS             14748..16097
                     /locus_tag="ONT85_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCR2378229.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(16142..16573)
                     /locus_tag="ONT85_001243"
     CDS             complement(16142..16573)
                     /locus_tag="ONT85_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR2378230.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            16961..19603
                     /gene="alaS"
                     /locus_tag="ONT85_001244"
     CDS             16961..19603
                     /gene="alaS"
                     /locus_tag="ONT85_001244"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HCR2378231.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            19803..20399
                     /locus_tag="ONT85_001245"
     CDS             19803..20399
                     /locus_tag="ONT85_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HCR2378232.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            20521..21669
                     /locus_tag="ONT85_001246"
     CDS             20521..21669
                     /locus_tag="ONT85_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HCR2378233.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(21707..22120)
                     /locus_tag="ONT85_001247"
     CDS             complement(21707..22120)
                     /locus_tag="ONT85_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HCR2378234.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            22199..22630
                     /locus_tag="ONT85_001248"
     CDS             22199..22630
                     /locus_tag="ONT85_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HCR2378235.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            complement(22729..22962)
                     /locus_tag="ONT85_001249"
     CDS             complement(22729..22962)
                     /locus_tag="ONT85_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HCR2378236.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            23145..24815
                     /locus_tag="ONT85_001250"
     CDS             23145..24815
                     /locus_tag="ONT85_001250"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HCR2378237.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            24962..25348
                     /locus_tag="ONT85_001251"
     CDS             24962..25348
                     /locus_tag="ONT85_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2378238.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            25335..26033
                     /locus_tag="ONT85_001252"
     CDS             25335..26033
                     /locus_tag="ONT85_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HCR2378239.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            26038..26502
                     /locus_tag="ONT85_001253"
     CDS             26038..26502
                     /locus_tag="ONT85_001253"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HCR2378240.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            26499..27410
                     /locus_tag="ONT85_001254"
     CDS             26499..27410
                     /locus_tag="ONT85_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HCR2378241.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            27645..28184
                     /gene="pyrR"
                     /locus_tag="ONT85_001255"
     CDS             27645..28184
                     /gene="pyrR"
                     /locus_tag="ONT85_001255"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HCR2378242.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            28198..29481
                     /locus_tag="ONT85_001256"
     CDS             28198..29481
                     /locus_tag="ONT85_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HCR2378243.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            29494..30420
                     /locus_tag="ONT85_001257"
     CDS             29494..30420
                     /locus_tag="ONT85_001257"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HCR2378244.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            30439..31719
                     /locus_tag="ONT85_001258"
     CDS             30439..31719
                     /locus_tag="ONT85_001258"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HCR2378245.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            31724..32800
                     /gene="carA"
                     /locus_tag="ONT85_001259"
     CDS             31724..32800
                     /gene="carA"
                     /locus_tag="ONT85_001259"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HCR2378246.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            32805..35990
                     /gene="carB"
                     /locus_tag="ONT85_001260"
     CDS             32805..35990
                     /gene="carB"
                     /locus_tag="ONT85_001260"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001126118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HCR2378247.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            36044..36820
                     /locus_tag="ONT85_001261"
     CDS             36044..36820
                     /locus_tag="ONT85_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HCR2378248.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            36817..37749
                     /locus_tag="ONT85_001262"
     CDS             36817..37749
                     /locus_tag="ONT85_001262"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HCR2378249.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            37746..38462
                     /gene="pyrF"
                     /locus_tag="ONT85_001263"
     CDS             37746..38462
                     /gene="pyrF"
                     /locus_tag="ONT85_001263"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HCR2378250.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            38469..39158
                     /locus_tag="ONT85_001264"
     CDS             38469..39158
                     /locus_tag="ONT85_001264"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HCR2378251.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQIEARLMKIEK
                     SDLCLQNHQLE"
     gene            39131..39400
                     /locus_tag="ONT85_001265"
     CDS             39131..39400
                     /locus_tag="ONT85_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2378252.1"
                     /translation="MSPESSIGIAKIQKLIARSIFSSGMKRNFYRDQFLIFLKFIFLR
                     LVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYLMI"
     gene            39438..40079
                     /locus_tag="ONT85_001266"
     CDS             39438..40079
                     /locus_tag="ONT85_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HCR2378253.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            40122..40814
                     /locus_tag="ONT85_001267"
     CDS             40122..40814
                     /locus_tag="ONT85_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HCR2378254.1"
                     /translation="MDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKGEETK
                     IEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSEHIIN
                     GKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVNPDVF
                     FPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPIQPLN
                     GREIYYFEEFEQ"
     gene            40811..41713
                     /locus_tag="ONT85_001268"
     CDS             40811..41713
                     /locus_tag="ONT85_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR2378255.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(41871..43577)
                     /gene="efbA"
                     /locus_tag="ONT85_001269"
     CDS             complement(41871..43577)
                     /gene="efbA"
                     /locus_tag="ONT85_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein EfbA"
                     /protein_id="HCR2378256.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
BASE COUNT        14019 a         7704 c         9493 g        12516 t
ORIGIN      
        1 acaaattatg ataattaagc atactatcag ataactcaaa atgaagcctt ctttcaaaaa
       61 attattcaaa accatatatt gtatttttta tttgataata acaatgggat tattaagaag
      121 tggcactaaa taaaagacta gtgtgtattt agccgattta attataaaca agtaaatgga
      181 taaagtatat gtaatgataa aagaaaaatg gattttctaa tttttaagct aaatgttaga
      241 tcacacatgt atatgctgga tagtcttata aaaaatcatg taatacagct tggtacatga
      301 tttttatttg tagctgtttt ttacatagac acaaaaaaga aaccacgctt gtagacgtgg
      361 tttctttttt actatctgtt tccagaagct gaggtgctgc tggatggtgt atggtcattt
      421 gcagcatccg ctccgctagt tgctggctca ctggaagaag caggaggagc agatgaagac
      481 ggtgtcgagc tttcttcgga tgaagaagaa ggaaccgttg ttgaagacgc tggctcgctt
      541 gtttctgctg tcgtgctttc ttttgaggac tcagcagttg attcgctttg actagtagag
      601 ctagagcttt gagtcgttgt ctctgttgtt gagcttccag gagtctggac atagctggaa
      661 gatggaatag ttgttgatgg tgtaattgca ttcgatctag ctgtatattg atctttgtag
      721 tacagttcac cgcccacacg aattaatccg cttggcatct cccagtctgt atttgtcacg
      781 ttagctgaaa catactgcat aagctcgcga taaacatcag aggctacgtg tgacgattct
      841 gaagtgacag gtgtcatttt tttattgtaa cctgtccaca cggaaatcga ataatttggc
      901 gtatagcccg caaataggat atctggatac accccagaag aaataccaag ttttgcatac
      961 tcgtcatctg tgtaattgga tgttccggtc ttaccggctt gatataaccc agcgatttgc
     1021 gcattcgtac cagttccttc agtgatcgta tcttttaaaa tgtctgtgat catataagct
     1081 gtttctggag acatggctgt ttttccatct ggctcataag tttcttctgt tccgtcttgg
     1141 aagacgattt tattaacata ttgaggtttg taataagtcc ctccattggc gaaagccgca
     1201 taggctgctg ccatttttaa ggaagaagct ccgtattttg tcccatcttg ttcttccgta
     1261 ttactagaga tcgcattcga ttgatggatc gtactgtatt cgatcccaag attttttaag
     1321 aaagaagcca ctttatctga accgacttca ttgaataatt tgactgctgg aacattacgg
     1381 gataagtaaa gtgcttggcg taaagtaatg gtgcccatat attgattatc ccagtttcct
     1441 acaggagtcg aagtcccttc atagttatac ggtgcgtcag taatagtttt tccagtagaa
     1501 tatttcaaat attcaaaagc tggtccgtaa tctgtgacag gtttcatcgt agaaccaaaa
     1561 tcacgggaag tattcactgc taagttgttg cctaaagtta cgtcttctgc gatgtgacgt
     1621 ccgccaatct gggcttttac tttcccggta ttggtgtcga taagggtgga ggcaacttgc
     1681 atctcgtcgt ctgggtaaga gacatactga tccgtgttga cgatatcata tagtttcttt
     1741 tgggcgtcca gatccaaatt cgtataaatc tctaaaccgt ctgtgtagac attcttatct
     1801 gttttttctt gtacttcatt gatgacttct ttgacataat tatcgacgat cttagtattg
     1861 tcatcggatt gagttagttc ttgcagacca tccgttaccg gtacattaac agcttgatca
     1921 tattctgttt gagagatttt ttcgttttga agcatcgtat acaaaacagt atctcgtctt
     1981 ttcttcgcct gatctggata aacataagga tcataggcgg aaggggcttg aggcatccca
     2041 gcaagcaatg ctgtttgcgg taaagaaagt tctgataatt tcttaccaaa gtacatctca
     2101 gaagctgtct ccatcccgta caagccgttt gacatataca ctttatttac gtagtaggtc
     2161 aagatctctt gtttcgattt tttttgttcc aatcttacag ccatccacgc ttcttgggcc
     2221 ttgcgtttca gagtctgatc ctcggcactg gtggagaaga aggaaagttt gatcaattgc
     2281 tgtgtcaaag tacttccgcc ttgcagtccg ccagaggtaa agttagagag ggcagaaccg
     2341 atgatccgaa tagggtcaac cccgatgtgt ttataaaaac ggcgatcttc taccgacacg
     2401 atcgcatctt ctaatgtttt cggtaattca ttcgcagaaa ttttttcacg cttttctgcg
     2461 cctaaatctt cgaatagttc gccatcttgt gtatatagtt tggaagatac ggtagcgtct
     2521 aattttttat ccgtcaattc gggcgcatct tttgcgtaat accagaaaag tcctacacct
     2581 gctaaaaaga ggatacagaa aaatgagagt attcctaaaa ggatcttgat aaataatcct
     2641 tttttatgtg ttccagagga ctttccagaa cctttgccag aattcttctt cacactcttt
     2701 ttaggagtag gctgtttctg tcgtctagag cttctagttt gttcatttgc cattcttgct
     2761 ttctccttca atagataatg ttgataaata gaagtcaacc gcgtccaaat aaggaatccg
     2821 cggagaaata ccagttttga tctctattcc tagttcttcg atcaaagata aaggtagtga
     2881 tttgcgtcct ttttcctttt gggcgtccca gtaagcaatc aaatgaacac tattcaaata
     2941 aaaacagcgc ttcaaagacg aaaaccataa caagacaaag cagaccccat tttgttcgag
     3001 gcatgctttc atatgttcga tttgatgggc atggaagttt ttaaagggaa aagatgtttt
     3061 gttcttggtt tcttttgctt caaagtccaa gtaaaagcct cgatagacgc cattatagtc
     3121 tgtagttgag gcttgggtaa agtaggcttc cttgatcaca gcagcacttc tcttaggata
     3181 gtctactttc acgatttgga taggggtagg ctttttatgg ataaccgcca acccgcggga
     3241 aagataatat ttattacttt cattgattgc ttcttcaaag cgcatccccc gattgccgaa
     3301 ttcagtgatc tgatttcttt tttcttgaat tttttcgacg ggagaggcat tcttataata
     3361 aggttggcca tttggatagc gcaataccat ctttaatcac actcctaggc cacattatag
     3421 caaaaaagcg gatagaaaga aaaggaatct gagatggata agtcaaaagt aatctatgtg
     3481 tctgggtatc gaagtttcga actcggtgtt tttaaagaaa atgatcctaa aatccaagtt
     3541 gttaaaaatg ttttaaaaaa agaactccat cagttagcag aagaagggct ggaatgggta
     3601 cttgtcagtg ggaatttggg cgtcgaacta tgggctgtgc aaatagtagc ggaattgaaa
     3661 gaagaatatc cggaactatc tcttggtatt ttgtatcctt tttctgattt tggtaataac
     3721 tggaatgaag ggaaccaaga aaaaaaacaa ttagcagaac aattagcaga ttatgtcgaa
     3781 gctgttagcc atcaacccta ccaatcacct agtcagctaa agaatcacac tcgttttata
     3841 cttgagcata cagtgggctg tctgctgatc tatgatgaag aattccctgg aaaaacgaag
     3901 tatttttttg aagatgcaca gaattaccaa gcagatcatc cctacgaaat tcggctgatc
     3961 agtatggacg atcttcaaaa ttttagtgaa taatcgatat tgtgtttgat ttatcaactt
     4021 ttttcctata caatgaaaaa ggtagaaatg ttattacgta aatgaaaact agaggtgtaa
     4081 atatggcgaa tttggtttat agtcctaaag acattttaca acaagaattc aaaacaaaaa
     4141 tgcgcggcta tgatcccgtt gaagttgatg aattcttaga caatatcatc aaagattatg
     4201 aagcttacag caaagaactc cttgctttac aagaagaaaa cgatcgattg agtgcaaaag
     4261 tagctcaatt atctaaaact caaggagcag ctcaaacacg cgtgcaacaa acagaggcac
     4321 caaagagcgc agcagtgacc aattttgata tcttaaaacg cttgtcaaat cttgaacgtg
     4381 aagtatttgg taaaaagctt gatcaacaag caagtgcagt caaaccagca caacctaatc
     4441 caaataacta cacaaatgca gacacaagct tagatgataa tgaaaaaaca cgtcaatttt
     4501 aatttttgat taatgaattt gcggttttcg ggtaatcgcg gtctgacttg atcaggctga
     4561 ggaaagtcca tgctcgcaca ggctgtgatg cctgtagtgt tcgtgcttag cgaaaccata
     4621 agctaaggta ctctttggag taacggcagg aaaacaagct aaggcttcgg ctatgcttaa
     4681 gtatccttga aagtgccaca gtgacgaagc ggctggggaa acccatccgg tggaacgcgg
     4741 taaacccctc gagcgagcaa cccaaacaat ggtaggggcg ctttttgtct ggaagaagaa
     4801 caggacaaaa aggcagtata actgcagata gatggttacc gctttgtttt ttctccctgg
     4861 aaaagcaaag tacagaacat ggcttataga aaaccgcaaa catcagggaa ttaggtcgtg
     4921 acaaaagtaa tgcgttataa gaaacaagaa aaacatttga aagctatttt tcaatacttc
     4981 agatgaatcg ttcttattac aaaacaaact tttgtatcac cggttattcg gaatttgagt
     5041 aaaagtgtgg ggtaggactg atgtcccacc tccttttttt ataaataagg agattgtgta
     5101 tggaaaaaaa caggaagttc aacctcgtcg caactgcggc aagcgggcta gaagcactgg
     5161 ttggtaaaga attaagagat cttggaatcg aatgccaagt tgagaacgga cgtgcacgtt
     5221 ttgaaggaac aatggaaacg atcgctacag ctaatttatg gttacgtaca gcagatcgag
     5281 taaaaatcgt ggttggggaa ttcgatgcgt atacgtttga tgaattgttt gaaaacgtaa
     5341 aagcattgcc ttgggaagat tttcttccac tagatgcagc ttttcctgta gctggaaaat
     5401 cgatcaagtc gaaactatat agcacacctg attgccaagc gatcacaaaa aaagcaatcg
     5461 ttgaacgtct ccgtacctat tatcaccgtc cagcttctgt accactgacg gaaactggcg
     5521 cacattttca gttggaagta gctttgctga aagaccatgt gatggtaact ctggatacga
     5581 caggaccaag tttattcaaa cgtggttatc gcttagaaaa aggtggagca ccattgaaag
     5641 aaaacatggc agcagcactt attatgttga caaattggcg aaaagaccgt ccattttatg
     5701 atcctgtatg tggttcaggt acgctttgta ttgaagcagc cttaataggc cataatatcg
     5761 caccaggatt caatcgtgag tttgcttgcg aatcttggga ttggttcagt caagaaatca
     5821 tggaaaatgt tcgatcagca gccgaagaaa aagcagatta tgatattgaa ttagatatta
     5881 ctggttcaga tatcaatgga cggatgattg aaattgccaa agccaatgca gaagaaatcg
     5941 gattaggctc ttcgattacc tttaagcaac aagcagtcaa agacttcaaa acggataaag
     6001 aatacggggt catcgtggct aatccgcctt acggagaacg tctaggagaa gaagaaactg
     6061 ttcggaaatt atataaagaa atgggcgatg tattccgtcc attaaaaaca tggagtaaat
     6121 atatcttgac tagcgactta gcgtttgagg aattttacgg acaaaaagca accaaaaaaa
     6181 gaaaattgta caatggtgca ttgcgtacgg atctattcca gtattgggga actcgtccgc
     6241 ctagaaaaaa agagaaagac gaataatctt actaattgat tcattcaatt ggatgtttgg
     6301 aggaataggg aatgaaagaa aaagagtttt tacaagaagt aaaagaaatc aatcttctac
     6361 aacaagcttt aggaatcctc gattgggata cacaaacagg tatgcctgaa aaagctagtg
     6421 cataccgaag tgaagtagac agctatcttt acagcattta tttttcaaaa aaaatcggtc
     6481 caaaaatcaa agaagccatt cgctattttt ctgaacatcc ggaagaattg tcggaagttg
     6541 gaactgccgt ttttgccaaa gtaaaagaag agtatgattt ggaacatcgc gtaccggaag
     6601 atcttatgag aaaatataca gctgccgttt cttcagcaca cggacaatgg caaaaagcta
     6661 gagaaacaca acaattttct gattttcaac acgcattgaa tcaaaatatt gaattaacaa
     6721 aacagttgat accttactgg agaaaagatg aaaagacgaa ttatgatgtt ttgctgaatc
     6781 aatacgaacc cgggatgact gtcgaaatcc ttgataaggt atttgatcag cttaaagaag
     6841 gaattctttc gatccgccgt acgctccaag aaaaagggac tgaacctgaa acagactttt
     6901 taaatcggag aatgacgaaa aaacaacaga agagatttgt agtgaaagta atcgaacaac
     6961 ttggttatga ttttagtcgt ggtcgtttag atgacactgt ccacccattt atgactggaa
     7021 ttaatccgaa tgatgcacga atcacgacac gatggaacga aacagatttc aaaatggctg
     7081 tttttggagt gatccacgag gctggtcatg gtatgtatga acaagacatc gatgataaat
     7141 atgcgtatac accggtttat gaaggtacat caatgggtat ccatgaatca caatcgttgt
     7201 ttaacgagat cattattgga agtagtcgca gcttctggaa aaagcaatat cctttctttc
     7261 aagaatgtgc agaaggaaca ttcgatgata ttccttttga caaattttat cgttcattga
     7321 accatacaaa atcaagtttg gttcgtatcg aggcagacag tttgacttac cctctccata
     7381 tcatcattcg ttatgaaatc gaaaaaatga ttttcaatga aggggtagat gttgctgaac
     7441 tacctgaaat atggaatcaa aaatatgaag agtatctggg aatacgacca acaaatgatg
     7501 tggaaggcat cctacaagac gttcattggt caggtgcaag ctttggttat tttccctctt
     7561 atgcattagg gtacatgtat gcggcacaat tacttcattc tttgcagcaa gaaatagatg
     7621 tggaaaatgt tctagcgtca gacgattatt ctccaatcaa aaactggatg tcagagcata
     7681 tccatcaata tggtgcatct agaaaaccaa atcaattaat catggatgca acacatgaac
     7741 cgcttaatcc gcaatacctg atcgactata tgaaagacct gtattttaat gtatatcagg
     7801 taaaataaaa tacaatttac aaagatagct gaaattcatc taatctatag atgttttcag
     7861 ctatttactt aagaaagaag tggaacgaat ggataaatta caagtgatcg ctgattatag
     7921 tatggaccaa ttagcgcatg atcaaacagg acatgggagc gatcatacca aaagagttgt
     7981 caagttagct gaaaaaattt tggagacgga accgcaggca gaccgctttg tgacgcttgc
     8041 agcagcttac ctacatgata cgattgatga taaagtcgtt aaaaatgaga acgaggcaaa
     8101 gcaacagctt cgtgtattcc ttcgctcttt gccaatagta gaggaacaga tctcgatgat
     8161 tttttccatt atcgaaaata tgtccttttc taaaaatctc tctgaagcgg ccgaactatc
     8221 tctggaaggt aagatcgttc aagacgctga tcgtatcgaa gcattaggag caatcggcat
     8281 tttgcgtact gcttatttcg gcggtggaca tgggcacccg atttttgatc ctgaactgta
     8341 tccgcaaaca ttcaaggaca aagaaaatta ccgaaaaggc acgacagtga tcaatcattt
     8401 ttatgaaaaa ctgttcttgc tgtctgataa aatgaatact gattacggat atgaagaagc
     8461 caaacgaaga gaaaccttca tgcgtgaatt cttagaagaa ttttttctgg aatggtctat
     8521 tgacgatgaa aattttactc cagacaattg gatcaaaaat aacaaaaaat gatggaggtt
     8581 gtgacagaag tggtcagctc caagatattt cggatcattt tccgtaaaag ttacttctgg
     8641 aacactgttt accaagaggt cgtgagcctg ccgttcagct ccgagtcaca aggaacaatt
     8701 tcaaaaaata gcttctcgtg tttgatgaat attgtaactt gtgattcgga attggcagga
     8761 gaacaccgtt tattcggaag aataggactg tgacaagact tttgtcacag tccttttttc
     8821 gaattcaaca agtaaaactt ttaagaaata ttcagagagt aaaattaaaa attgctgata
     8881 aacgaagatg gaaattttat gtatttttat tggtctatgc aataaataaa ttaaatattc
     8941 agaatacaat tgattttaac gaaagggctt ctatcaatgg ttgtgttgta tttatctgcc
     9001 caggtagagt aaaatagatc attatctaaa aggtaattgt tcatactata taagtcattt
     9061 gaaagtaaaa agatactgta aataaaaaat gtggttaaat aattctcata atcagtgtaa
     9121 gcgttatcaa aaatatagca tacttgcttt gtagatgaat gacacgatat atgtttggta
     9181 tgttctgcaa gacagatgaa tatggaggtg gagacaatgt cttccaaaga actaacaatg
     9241 tatttagaga aagaattgga tcaactgaaa gaaaaggggt tatacaatac gatcgacata
     9301 ctagaaagtg aaaatggtgc ttgtatcatc gtagacggaa aaaagatgat caatctagca
     9361 tccaacaatt atcttggatt tgccaatcgc gaagaattga aaaaagcatg tattgaagca
     9421 accgaaactt atggtgtagg agcaggagca gtccgaacga tcaatggttc gctgaaaatc
     9481 catcagcaat tagaagaaaa aatcgcagaa ttcaaaggga cagaagcagc aatcgccttt
     9541 caatctggat tcaactgcaa tatgggcgct atctctgcag taatgacaaa agaagatgct
     9601 atcttatcgg atgaattaaa ccatgcatct attattgacg gttgccgctt atccggagca
     9661 aaaatcatcc gaattaaaca tcaagatatg aaagaccttg agaaaaaagc aaaggaagcg
     9721 atagaaagta agaaatataa aaaaatcatg tatatcacag atggtgtttt ctccatggat
     9781 ggagacattg cacgattgcc ggaaattatt ccgattgttg aaaaatatgg actgatcacc
     9841 tacgtcgatg atgcgcacgg ctcaggcgta actggaaaag gtgcaggaac cgtcaagcat
     9901 tttggattaa gcgacaaaat cgatatgcaa atgggcacgt tatcgaaagc tataggtgtt
     9961 gttgggggtt atgtagctgg atcgaaaaca ctaatcgact ggttgaaagc tagaagtcgg
    10021 ccattcctat tttctacttc gctaactcca ggtgcagctg cagctgcatt ggcttcgatt
    10081 actttgatgc aagaacatcc ggaacttgtc gaaaaagttt gggaaaatgc taattatttt
    10141 aaagaagaat tgaaaaaagt aggctataac atcggtatgt ctgaaacacc tattactcca
    10201 gtcattttgg gagacgaaaa agtgactcag actttctcta agaagctgat cgaacatggg
    10261 atttatgcta aaccaatcgt ttatccaact gtgcctcttg ggacaggcag gatcagaaat
    10321 atgccgactg cggaacatac gagggaaatg cttgatgaag cagtagcagt ttatcaaaaa
    10381 ataggaaaag aaatggaaat tatttaacta aatttagttt tttcgtctat ttaaaattag
    10441 agaacatgtt cacgcatata ttgttaatca tctgaagata gtagtaaaaa agatcaggga
    10501 ggatcatcat gaaaaaagta ttggtgacag gatgtttagg acaaattggt tcagagttga
    10561 tcacacggct acgtcaagaa ctaggcagtg aaaatgtcat tgcgacagat atacgcatgc
    10621 ctgaagtcat tgagaaaaac gatatctttg agatcctaga tgtaatggat tacgaaaaaa
    10681 tgcgcagcat agcagaacaa tatcaagtgg acacattgat tcatttggct gctcttttgt
    10741 cagctgttgc agaagcaaaa ccacaattag cctggaattt gaacgtgact gggctggtca
    10801 atgcattgga agtcgctaga gaattccagc tgaaattttt tacgccaagt tcaatcggcg
    10861 cgtttggtcc aaatactcca aaagatcata ccccacaaga tactcttcag cgtcctacaa
    10921 ccatgtatgg ggtaacgaag gtagctggag aactactatg tgattattat tatgtgaaat
    10981 atggcgtaga tactcgtggt gtccgtttcc ctggattgat ttcttatgca accttgccag
    11041 gaggcggtac gacagactac gcagtcgata tttattattc agcaatcaaa gaaggaaagt
    11101 acactagttt cattgatcaa ggtacatcaa tggatatgat gtatatgcca gatgcgatcg
    11161 atgccattat aaaactcatg aatgcagatc ctgatcgatt ggttcatcga aatgccttca
    11221 atatatcggc gatgtctttt gacccggaag agatcaaagc atctattcaa aaaatcatac
    11281 cagattttga aatggattat gatgtggatc ctgtacgtca gtcaatagca gaatcttggc
    11341 caaattcact agatgtttct tgtgcgcaag aggaatgggg cttttcgccg aaatacgact
    11401 tagatgtgat gacaaaggat atgttggaaa aactgaaaga aaaattggta gagacggtat
    11461 aaaacaagaa ccacatccaa aaagtttttt atacttttgg gattggctat ggtatgaaag
    11521 gggacaaggg atggaagaat atcaagccac gccagtaaaa gaagtaaaaa tagaaaatga
    11581 attaaaacgt acgatgggat tttttactgc tttatcgact gttatgggca cagtgatcgg
    11641 tgcaggggta ttttttaaag ccgcaagcgt agcagaagtt acaggatccg ccagtttgca
    11701 tatgttttct tggtttctcg gtggtatgat ttctgtctgt gcaggactaa caggagctga
    11761 actggcagct gctattccag aaacaggagg gatgattaaa tatatcgaac ggatttacgg
    11821 aaatacagca gcttttttat tgggctgggc gcaagtcgtg atttattttc cagcaaatgt
    11881 agcagccttg tcgatcattt tcggtacaca gttcgtcaat ctatttggtc tttcccagtc
    11941 aatgattgtt cccgtagctg ttacagctgc tgtatcgatc ctgttgatca attttttagg
    12001 ttctaaagct ggaggggctt ttcagtcgat tacgcttgtc tgcaagctta ttcctttgtt
    12061 tgtgatcgtc attttcggtt tgttccgaca agaaggagta gacttccaac tgtttccgat
    12121 ccaagcagga gaaaatcttt catttttctc agccttggga gcgggcttgc tagcaacgat
    12181 gttcgcatac gacggttgga tacacgtagg aaatatttcg ggagaattga aaaagccggc
    12241 gaaagatttg ccaaaagcaa tttctttagg aatcatcgga atcatgatcg tttatttatt
    12301 agtaaatgcg gtgttcttaa gaaccgcatc tatcgacgga gtcgcaggaa acagcaatgc
    12361 ggcaagtgac gttgcaaaaa tgatttttgg cggcttcggt ggaagactgg tcacagttgg
    12421 tatcttgatt tctgtttatg gaaccatcaa tggctatacc cttactggaa tgcgtctccc
    12481 ttatgttatg gcaaaagaaa acaacctgcc ttttagcaag ctctttgcta aacttcatga
    12541 taaaactaaa gttcctgtag ccgcaggtat cttagagcta gtcatcgcta ttggcatgat
    12601 gatgatcggt ggttttgata cactaacgga tatgctgatc tttgtcattt ggatttttta
    12661 tacgatggtc tttgttggtg tcattcttct tcgtaaaaaa gaacctgatt tgtttcgacc
    12721 atacaaagtt ccaatgtatc cgtttattcc actagtagca atcattggag ggacttttat
    12781 cgtatcaagc acgcttatca ctcagacctt tttagcttcg atggggatag tattgacact
    12841 tgctggtatc ccgatttatc tctatttaaa acgtagatag ctaaaacatt cgaattttat
    12901 tagaactgca cttctctgcc aaacaaaata ttttatttaa atgaggagta cgtggaagaa
    12961 atgtcaaaag tactcgttaa ttttgatcac gtaaatgtcg ttttgtctac gatgaaatag
    13021 caaagtaaaa gacagaaatc gggctcaacc gatttctgtc ttttactttg atttgcgtaa
    13081 ctgaactatt cggcgtacgc cgcgataaat atttaataaa accaagcaac cagaaatgaa
    13141 aatgggaagc caaatcagat agacagggta gccaagcact ttagacaaag catgtggagt
    13201 cattttatcc atttcaagaa gtgcttgctg ccaagtctga agattgaaga ctagcatgaa
    13261 acccgaaata atcaggagaa taaatcctaa ataatgaatt gtagttcttt ttcttacata
    13321 agcgatcgta gcataaagga acagtaaaaa aataggtacg ccaaaaacaa agtacatatt
    13381 cagcatctcg acaccccctt tgtgtttatc ttactttact ttgaaagaaa tgagaaataa
    13441 tagcctcgtt acttgcttga aacagcaaag gactgctaag ataagagaga tacaaagaat
    13501 caaggaggag tcatatggca agttatatcg aaccgattcg ttttgcgctg attgtttttc
    13561 catttttagc cttagcgatc tcatctgttt tttttattta tgaatatcga aagtatggaa
    13621 catttctagt aacaagggca gttattttgt attctttcgt tttttattta ttgtgtgcgt
    13681 attttttggt gatcttacca cttccggcaa aggagacggt tgcccaaatg acagggccaa
    13741 agatggagct gcatttattt gcttcatggc aacatttcag agacaacacg gtattggtct
    13801 tgtctgaacc tagtacctac ctgccggcaa tgaagcagag tgtatttttg gaaccagctt
    13861 ttaatgtttt attactcata ccatttggca tttatttaag atattatttc cggctttctt
    13921 ttatgaaaac gatgattgct agtttctgcc tttctctatt ttttgaattg acgcagctaa
    13981 gtggacttta ttttatctac ccaagaccat accgcttatt tgatgtgaat gatttagtga
    14041 caaatacatt gggtggaatc attggttttg tcgttacgcc gttatttaca tttatgcttc
    14101 ctacaagaag acgaatggat gaactttctt atgaaaaagg aaagactgtc tcattgactc
    14161 gacgcttggt cgcttatttg atcgactgga catttttatc tcttattaat ttggtagcgg
    14221 cgattttctt taaaattgcg ttcgattcag gggcagttac ttctcatatc tggtggcttt
    14281 tattagaagt aggggtatac tttatgattg taccgtttat aaccaatgga tggacacttg
    14341 gcaaaaaaat tgtgcgtatc cgtttggtgg aagaagaccg cgaacgaatt tctttaaaag
    14401 cattgatgat ccgctacggg tatctgtact ttctttttta tggtttaagc tggttggggc
    14461 agttgcttca gcaaatcatg aattcgacaa cgaatacggt gttgcagatg agtgcagtat
    14521 tcatttctct cggtgtcttg ttactacaag gtatttttat tttaaatatc ttgctatcat
    14581 ttttgagaaa aaaacggatt ctgttttatg aaaaagctag tcacacatat actgttagca
    14641 cgattggcca aacaaaggat gatgattctc gaaattagct gagcaaaaac gctttgtctg
    14701 tgttataatg ccaaagtcgt gataatttaa agagaagagg tgcacgaatg aataattttg
    14761 atatattccg cttcaaaccg tttatcaatg aagcgttaac cgcaaagggc ttcaaacagc
    14821 ccacagaggt ccaggaacgt ttgattcctt taattgcaaa aggaaaaaat atcattgggc
    14881 aatctcaaac tggtacagga aaaacacata cgtttttgtt gcctttgttt gaaaaaatca
    14941 atgcagaaaa agaggaagta caagttgtta ttacagctcc aagtagggaa ctagctgcac
    15001 agatctatga agcagctaca caaatcgcta aattttctac accggagatc cgtgtctcga
    15061 attttgtcgg cggtacagac aaacagcgtc aattaaatcg tctgcatcac caacagccac
    15121 aggttgtagt aggcacgcct ggtagaatat tagacatgat gaatgaacaa gcgctgaaga
    15181 ttcacaccgc atttgcattt gttgtcgatg aagcggatat gacattggat atgggttttt
    15241 taaatgaagt agaccaaatt gcaagtcgat taccagaaaa gctgcagttt ttagtttttt
    15301 ctgccactat tccagaaaaa cttcgtccat tcttacgaaa atacatggaa aatccaatca
    15361 ttgaagaaat caaaccaaaa gcagttattt ctgaagacat tgaaaattgg ctgatttcaa
    15421 caaaaggtaa agatgagaat caaatgattt atcaactgct gacaattggt catccttatt
    15481 tagcaatcgt atttgccaat acaaaacagc atgtggatga aattgccgat tacttgaaaa
    15541 accaaggctt gaaagtagca aagattcatg gagacatcac cccaagagag agaaaacgag
    15601 tgatgcgaca agtacagaat ctggagtatc aatatgttgt agcgactgat ttagctgcta
    15661 gagggatcga tatcgaaggt gtctctcatg ttattaatgc agaaattcca gaagatctag
    15721 acttctttat ccatcgtgtc ggacgtactg gacgtaatgg cttaaaagga acggccatta
    15781 cgttatattc gccaaatgat gagcaagcca ttgatgcgat agaaaaattg ggcgtctcat
    15841 ttgaaccaaa agaaatcaaa aacggggaaa tcgtcaaaac atacgatcgt aatagacgtg
    15901 ccaaacgaga aaaatcaaaa gatgtattag atcccacgct gattggtctt gtgaagaaga
    15961 aaaagaaaaa gatcaagcct ggttataaga aaaaaatcga ttgggcaatt gcagaaaaaa
    16021 ataaaaaaga acgcaaaatc gaacgcagac agcaaagccg tactgctcgt aaaaataaga
    16081 aaaattcaca taaataaaaa acaagaggtt gtgacaaaag gcgtgtcaca atctcttgtt
    16141 tttaagctag actttttttc attccgatac aaagtacttc atcttcaata aaaggttctg
    16201 tgcaagtatg atacccttgt ttttcataaa aatctacagc cgtcatttca gctactacat
    16261 aactagtctg acaaccttct gaaaccgccc gtttttcagc agcttcaagc aactgaccgc
    16321 ctaacccttt tcctcggtat tcttttgcta cgcaaaatcg ttccggcttg aagcaagtag
    16381 gtgttttctt ttcataacgc aaagtagcta ctggtctttt ttgatcaaga aatagataat
    16441 aggtttgttg cgggatatct tttccgtcga attctagttc tgcaggaatc ccttgttcta
    16501 atacaaaaac ttgatatctc aaaaagcagc tagctgcgcg tatccattca tccgctccaa
    16561 ctattttttt catcatattc ctccttgtca tgaatagcag agatgtattg attcctttat
    16621 tcttatcgta ccaaaaatcg tggagtatga ccaaaaggaa atgaaaataa aaattgagat
    16681 attgacagtg atcaatagaa tgcgtatact aattaagtac gaaaaggata tgatttgttt
    16741 tttaagactc acagaaaatt ctttcttggc tgaaaaaaga atagtcacta atcaaattgc
    16801 tccctttttc tattttggta gaaataccga cgcagctcag cgttatcgag tttaagaggt
    16861 aatgattaca catcattgca atcaaggtgg taccgcgagt tttcgtcctt ggctgtgatg
    16921 gtgttttttt gtatttaatt tatagaaagc aggtaatgac atgaaacaat taagtagtgc
    16981 acaagttcga caaatgtttc ttgatttctt ccaatctaaa ggacatacga ttgaacctag
    17041 tgcgtctttg atccccgtga acgatccaac cttattgtgg atcaactctg gtgttgcaac
    17101 attgaaaaaa tattttgatg gttcagttgt gccagaaaat ccaagaatca caaatgccca
    17161 aaaatctatt cgaacaaatg atatcgaaaa tgtcggtaaa acagcacggc accatacgat
    17221 gtttgagatg ctgggtaatt tttcgattgg tgactatttc aaaaatgaag ccatccactg
    17281 ggcatgggaa tttttgactg atccaaaatg gatggcattc gatccagaaa aactttatgt
    17341 aacagtttat ccaaaagata cagaagcaaa acgcatctgg catgaagaag ttggcctgcc
    17401 actagatcac attattgatg tagaagacaa cttctgggat atcggtgcag gcccatgtgg
    17461 tccggatacg gaaattttct atgaccgtgg agaagcttat aacgatttag cagaagacga
    17521 tcctgaaaac tatccaggag gagaaaatga acgttattta gaaatctgga acttagtatt
    17581 ttcagaattc aaccataagc ctgataacac atttgaacca cttcctcata aaaacattga
    17641 tacggggatg ggattggaac gcatggtctc aatcgttcaa gatgcgtcaa cgaactttga
    17701 gacagacttg tttatgccta ttatccacgc cgtagaaaag ctaagcaaac aagtaaaata
    17761 tggtgaaaac ccagtaacag atatttctta caaagtaatc gcagatcata ttcgcgccct
    17821 ttcatttgct attggcgacg gtgcattgcc atcgaatgaa gggagagggt atgtcttacg
    17881 ccgtttgctt cgtcgtgctg tcatgcatgg gaaaaaatta ggtattgaac aagcattctt
    17941 atatcgttta gtccctgttg ttggggaaat catggtaagt tattatcctg aagtacttca
    18001 acaaaaagaa tttattgaaa aagttatccg tacagaagaa gaacgtttca atgaaacaat
    18061 caatgaaggt ttggaaatct taaatcaagt gatccaagaa gtaaaaaacg aaaacgggac
    18121 gactctagca ggaaaagaca tcttcaaact atacgacaca tatggcttcc cggtagaatt
    18181 aacggaagag gtggcagaag atgaaggact gaaagttgat catgctggtt ttgaaaaaga
    18241 aatggatgca caacgcgaac gtgctcgctc tgcccgcagt actgaaaaat caatgggggt
    18301 acaatctgct ttattgacag atatcaaagt agaaagtaaa ttcattggat acacagaaca
    18361 agaatcagat agtcaattat tggtcatcgt taaaggtgag tctcttttga atgaagtcgc
    18421 agaaggtgaa gctcaaatga tctttgatca aacacctttc tatgcagaaa tgggtgggca
    18481 agtagcggat caaggatgga tcatgaatga agtaggcgaa atcgtagcag ttgttacaga
    18541 tgttcaaaaa gcgcctaatg gtcaatttat gcacaccgta gaagtaaaag caccaatgag
    18601 tgaaggacaa acctatcatc tattgatcga tcaatcgatg cgcaatcgga tcatcaaaaa
    18661 ccatacagca acccatctac tgcacaaggc attgaaagaa gtacttggcg agcatgcaaa
    18721 tcaagctggt tctttagtcg ctcctggaca tttacgattc gactttaccc actttggtca
    18781 aatcacttca gatgaattag ctgaaatgga aagaatcgtc aacgaaaaaa tctgggaagc
    18841 tatcccagtt gaaacaattg aaacagatat cgatacagcc aaaaacatgg gtgcaatggc
    18901 tttgtttggt gaaaaatacg gacatgacgt tcgagtagta aatattggcg gctggtcaat
    18961 cgaactgtgc ggaggcacac atgtagcaaa tacagaaaac atcggaatct tcaaaatcgt
    19021 ttctgaatca ggaattggtg caggcgtgcg acgaatcgag gctgtaacaa gtaaagaagc
    19081 gtacgaacta cttgaagaag aagaaaaaca actaaaacaa gtagcaacaa ttgttaaatc
    19141 acctcaattg aaagaagttg tttcaaaagc ggaacaattg caacagcaag taagagagct
    19201 acaaaaggaa aatgaacaat tagctagcaa gttagcgaat caacaagctg gtgatgtatt
    19261 caaagatcca gaaacgatca atggaaaaac aatcattgca gcacaagtaa aagtgaaaga
    19321 tatgaatcag ctgcgtcaat tagctgacca atggaaacaa aaaaatattt cagatattct
    19381 tgttttagca aacgaacaag aaggaaaagt cagtctgcta gcggcaatga cgaaagaatc
    19441 aaatgaagct ggactaaaag ctggcgactt gattaaagca atcgcaccga aagtaggcgg
    19501 tggcggtggt ggacgtccag acatggcaca agctggcggg aaaaatcctg ctggtattcc
    19561 agatgcactt aaagctgcga aagaatgggt tgcgcaacaa tagggcgttt acgtttgaca
    19621 gaaaaacaaa agttcaaaca ttgaacgaaa catgattatg ggggcgaaga caaacaagtc
    19681 ttgccctcat acttttttca attggatggc gcatctaatc aaatggtcgg attcggatgg
    19741 tctttagttg acgaaagtga cagaaatttt tacgattagt ataaataaaa aggggtgaaa
    19801 aaatgaatag tagaaataag acctttcgac tagtcttacg tgcgatcttg ttagcaatca
    19861 tcatcgtaca agcgatggtt ccttggctgg ggtttattcc attaggattc attagtttaa
    19921 caattatcca tatcacagta attattgctg cagtagtatt aggcccaaaa gatggcatgg
    19981 tgattggctt gttttgggga atcgctacaa ttgttcgtgc ctatgccatg ccaacaacgc
    20041 catttgatac attagtgttc acgaatccga ttatctcggt agttcccaga gtacttgttg
    20101 gcttagtagc aggcttggtt ttccactgga tttatcaaaa aaataaatca ataacgatca
    20161 gctcagcctt tgcaggggta ctgggttcat tggtcaatac agtcttagtg ttaggattca
    20221 tgggattatt ttatacaggt gcaacggcag atgcctatgg tgtagactct gctcttttat
    20281 ttaaaacctt agcaggggtt gcagcaatca atgggatacc agaagcaata ggtgcgggaa
    20341 tcataacacc attactagca aaagcgttat ttgcagcaac accgttgaaa ccagaataat
    20401 gaccatcttt taatagtaat aggacgcacg atagatagaa aaaggaagag aggagaggaa
    20461 aacaatcgtc ctctccatct ttttacctga gcaacaagca aactagaagg ggtaaaaaac
    20521 ttggctaaga aacaagaaga aaaaaagaaa caaataccgc aacagcggac aaaaaaagga
    20581 gtactagcgg gtatcttagg attggttacg aatctgatct tgtttgtctc taagctaatg
    20641 atcgggcttt tttcaggaag tgtctccatt atggctgatg cagtgaacag tctatcagat
    20701 actgcttcat ccatattgac tttgatcgga tttaaaattg ctgcaaagcc agcagatcag
    20761 gaacatcctt atggacacga acgcttcgaa tatatcagtg ggttatttgt ttcgatcatc
    20821 attacttatg taggatttca atttttagag tcttctgctg aaaaaatcat ccatccagaa
    20881 aatgtctctt taacacccat cgtctttttt gtcctaatca tttccatctt tttgaaattt
    20941 gcacaaggca aaatgtatca ggttattgcg caatccattg attctgaaac attgagagct
    21001 acagcaacag atagttataa tgatgtcttt actacactgg ctgtattgct ttcagcagca
    21061 atcgaatggg taactggatg gcgtattgat ggatatatag gttttgcttt agcattatat
    21121 atcctttata gtggattgac gatgcttcga aattttgtat acgaactatt aggaagtcgc
    21181 ccaacgccag cagagattga agaaatggaa gagcatttaa atgcttgcga tgatatactt
    21241 ggctatcatg atttattggt gcataactat ggacccaata aacgctttgc atcagtccac
    21301 atcgaagtag atgaatcaat gaatttgaat gtggctcata agattattga tctgatagaa
    21361 aaagacttta aaaagtcttt ggatgtggaa cttgtctgtc acttggatcc aatgcctgtt
    21421 gataacgaaa aatactataa tatcttgcgg cagttaaagc aaattcttct tgaaacaggt
    21481 acagggctta atattcatga ttttagagta attcatcaag gaaaaacgct gcaatttgat
    21541 gtagtggtcc ctgagaaatt ccagtttgtt gatacagaac tagaaagaat cttacgtagt
    21601 caaatagaag aaaaaatcgg agattaccaa ttggaaatta cgtttgatca caattatctt
    21661 ttatattaaa tgagaaacta taaaaagact ccttaaagtt tttaatttaa agagtctttt
    21721 tttcttttaa ttgtttatgg agagcctgac gtgcaagatt atctgctcct ttatttttgt
    21781 tttcgggcaa ccattgcagg atcaataaag aaaattcgga tagaagttct tggattcttt
    21841 gaaggtaggg aaggaagagc tcgttcttcg ttgcgttttt gtcgaccgta gaaactagtg
    21901 ttttgctgtc tgaaaaacag aaaatcgttt cattctgtaa gtcgtttttc tttagaaatt
    21961 ctaaagtttt taaaaagact tcgaactctg cttgatggtt tgtccctata gaaagcggga
    22021 tagatagctg ttcttgttta ccttgataga caatcaagat tccacctcca ctaggaccag
    22081 ggttgccttt tgtagaggca tcaatgtata cttttaacat aaaaagagat tctccttttt
    22141 gttcaaacta atgttatggt aagattatat cattactgaa tcggtgagag gttagagaat
    22201 gaaaaaaaat attcgatggc agcctgaact ggcacatacg atcatctatt ggtcattgac
    22261 cttgatcatt ctgtttttta gtctgattct ttctttagaa aacacaggtc cttattggaa
    22321 aagcaatttt gttttaggta tttttcttgt ttttctattt ctaggtttgc ggcgttcttt
    22381 tattttaaaa tcaaactctg tgattatacg ttatgctgct ttttggaaag accgagaaat
    22441 atctgtcgga cagattcgat ctttgacgcc aactaatact ggggtgcaaa tgatgttgca
    22501 agatgagaaa gccccatctg ttttcatgat gcgaaaaaaa gatcaagtag tcctttttga
    22561 tcattttgaa aaaaacggac ccaaatcaga atcaattcct tatccttata cgaaaaaaag
    22621 aaaaagctaa ttttaaaaat cagaacctaa aaaaacgaat ttttacagga aaagaaaaga
    22681 gtgagacgta cggttcatcc cgtttagcgt ctcactctat ttgtttatct attcatgcat
    22741 actagtatcg gtagtctgaa cgttgccttt cttgacatgg gcagcttgtg ttcctcgatt
    22801 cccttcgatg atctcaaatt cgaccacttg attttcttcc aaagttttga atccctcttc
    22861 ttcaatcgca gtaaaatgta caaaaatttc ttctgtgtca tcataactga taaagccgta
    22921 tccttttttg ttgtcaaacc attttacaat tcctgtggtc aaagcactca cgacctttct
    22981 aaagtctgat catagccttc attataaaga gtatttataa gtacgtcaaa taaattaaaa
    23041 acctgtaatt ttatgttttt tttctgaata aaaaaaggta taattggcat atcttaacaa
    23101 agaagatatt ttgttgaata aagttcgtga aaaggggtaa tcaaatgaag aatgatctgc
    23161 aaatttcaca agaaacagag ctagaaccga ttgttgaagt ggctagtaaa ttagatctta
    23221 aggaagacga tttggaactg tatgggaaat ataaggcgaa aatcaatttt tctggaatca
    23281 atcgatcaaa ggataacgct gaggggcatt tgatcttagt tacttccatc aatccaacac
    23341 cagctggcga aggaaagtca acgatcactg tagggttagg agacgcgctc aataaaatca
    23401 ataaaaaatc tgttattgca ttgcgagaac cctcacttgg tcctgtgatg ggaatcaaag
    23461 gtggtgctgc aggcggagga tatgcacagg tattgcctat ggaagatatc aacttgcatt
    23521 ttactggtga catgcatgct atcacaacag ctaataatgc attatctgct cttttagata
    23581 atcatattca tcaaggaaat gaattgaaca tcgatgcccg ccgagtgatt tggaaacggg
    23641 tagtcgatct caatgatcgt gaactgcgta aagtaattgt tggcttgggt gggccaatcc
    23701 aaggtgttcc aagagaagat gggtttgata tcacagttgc cagcgaaatc atggctatct
    23761 tgtgtcttgc ggcagacctt gaagatttaa aggctcgtct tgcaaggatc gttgtcggct
    23821 atacatttga ccgtcagcct gtgactgctg gagacttgaa agcagaaggc gctctggcat
    23881 tactgctgaa agatgcaatc aagccgaatc ttgtacaaac aatttacgga acaccagctt
    23941 ttgtccacgg gggaccgttt gccaatatcg ctcatggctg caatagtatt ttagctacca
    24001 agacagcatt gagattagca gattacgttg tcacagaggc aggatttggt gcagatcttg
    24061 gcggagagaa atttttagat atcaaagtac ctaacttgaa aaaagcgccg gatgctgtgg
    24121 tgattgtcgc cactgtccgt gcattgaaaa tgcatggcgg aatgaaaaaa gatgaactta
    24181 aaaatgagaa tcttgatgct ttgaaagtag gttttgcaaa tttaaagcgc catatacgta
    24241 atatggaaca atatcagcta cctgtgatcg tcgcaatcaa tgaattcgta acagatacag
    24301 acaatgaact cacactgttg gagcacttgt gtgaaaatca ggggattctt gccaagagag
    24361 cgagtgtatg ggcagatgga gcagaaggcg gcgttgattt agcaaaagct gtagtacgat
    24421 tgattgatag aaaagaagct aattacaagc cattataccg cctagaagaa acgatacaag
    24481 aaaagacaga aacaatcgtc aagaaaattt acggaggaaa tggtgttgtt ttcagcaaaa
    24541 aagcgcaaaa acaaatagaa gagtttacga agaatggatg ggacaaactg ccgatttgta
    24601 tggcaaaaac acagtattca ttttctgatg atccttcact gctaggtgca ccggaagatt
    24661 tcaccatcac gatccgcgaa atcatcccaa aattaggggc aggattttta gtagccttga
    24721 ctggagatgt tatgacgatg cctggtttgc ctaaaaaacc tgctgcattg aatatggacg
    24781 tgacaaatga cggagaagtg ttgggattgt tttaagcttg attataatct accatcgaag
    24841 gagctgtatt ttgactacag cttctttttt tagacgatag ggaattaatt ttgtttacac
    24901 taggatcatt ctttttcttt attgtatttt gagctatact aaagcataat aggagggtat
    24961 gatgaaaacg acatttataa gaaaaaccgg tttttacggt atgggtagct cgattcagct
    25021 aagaatcaac aatgaaaaac gcagtgttct cagtcatgaa caaaagataa cgcttgatct
    25081 ggaagtgccc tttacgatgc aggtcacctt ttattggcta aaaagccccg tttatacagt
    25141 gaaagaagca aagcctgctt atgtgatcac aatgaattac ttgattcttc aaatctatcc
    25201 gtttttattt ctagcaactg ggttgggggc cgttttccta caaaatttat ttttcagttt
    25261 ttttgcagtt attgtgttga ttgcgttctt tttctatata aaaaaccggg catatttgat
    25321 caaggaggaa aattatggca aactctgatc tttttttaat cagtttcaat cgaatcgaaa
    25381 aatggatgag agacgagatg ggaaatgcac gaaatatggg atttaccgaa cttgtcagac
    25441 gcctagctca aagaaaacaa ttaatgatca gaaaatacga agatgatctt ttacagcttg
    25501 ctcagttaag gaatgcgatc gtccatgatc ggatcgcagt cgattttgtc attgcagagc
    25561 caaatgattg gacagtcaag cgaatccagc tgatcgaaca agaactgatt cagcctgaaa
    25621 ccgtgctgcc tcgatttgca aaacgtgtaa caggttttga acaagatctt ccgttacttg
    25681 aactgttaaa gatagtagca gaaaaaaggt attcacaatt tccgttatac aataaaggaa
    25741 aatttgtagc attgattact ttacgtaata tcgggttttg gctagcaaaa gaaagccaaa
    25801 aaggtccggt tgatttaaca aataaacgag cgttggattt gatcatccaa aacggaaagt
    25861 acacaaatta tcattttgtt ccagctaaaa cacatattta cgaggttgaa gcgatgtttc
    25921 gtgagcaagg gactttggaa gccgtactta tcactaaaga tgggaatccc gatggtaatc
    25981 tgctcgggat cgtacgacct cgtgatattt ataaacaaat agaaaaggat tgattttttg
    26041 ttaattattt attggatatt cagcgctttg ctagtaggag tggatcagtt ggtcaaatgg
    26101 tggatcactg ataatctagc gcttggagag acgaaaaatc ttataccagg tattttgtca
    26161 ctgaatcata ttcgaaacac tggagccgca tggagtatgc tagaagggaa gatgtggttc
    26221 tttactatag tgacactcat tgcagtcgtt gtgattctca cattgatgat taaaaatcgt
    26281 gaaaaaggta atcgctggtt catgattgga ttgagtttga ttctagctgg tgcgattggc
    26341 aattttatcg accgtattcg tcttggctat gtagtagata tgttccagac agattttatg
    26401 agctttccga tttttaacgt agcagatgtg acacttgtat gtggagtgat ttgtatgtta
    26461 atttatatta ttttagatga aaaggatcaa agaaaaaaat gacagaactt aaaacgacga
    26521 ttcaccatga atctggtagg atcgataaag tattgactaa tttattcacc gattacagtc
    26581 gttcgcaaat tcagctgtgg ctgaaaaatg gagcggtttc tgtcaatgga cagccagtca
    26641 aagcaaatta caaagtaaaa caaggagacg agattactgt cttgatcccc gaaccagaaa
    26701 cattagatgt ggtcccagaa aatatcccgt tgacgatcgt ttacgaagat gaagatgtag
    26761 cggtgataaa caaacctcaa ggaatggtcg ttcatccttc agcaggtcat acgcatggca
    26821 caatggtcaa tgcgctgatg taccatatca aggatctttc tacgatcaac ggcgttattc
    26881 gtccaggtat tgttcaccga atcgacaaag atacttccgg actgctaatg gttgccaaaa
    26941 atgaccaggc acacgaatca ttagctcagc aattaaaaga taagacctct ttgcgcaaat
    27001 atgttgcttt agtccatgga gagattcctc acgaaaaagg aaccatcaat gcgccaatcg
    27061 gccgttcgaa ggaaaaccgg aaaatgcagg cgataagaga agatgggaag tcggctgtca
    27121 cacattttac tgtattggaa cgtttcgaag gatttactct ggttgaatta aaactagaaa
    27181 ccggaagaac acaccagatc cgtgtgcata tgaagtatat cggttatccg ttagctggag
    27241 atccagtata cggaccgaag aaaactttga aaggcaatgg tcagtttctc catgcgcaaa
    27301 tgttaggttt tacgcacccg actacaaacg aaaagatgat ctttgaagca cctcttccag
    27361 aagtttttga aaaaacttta gaaaaattaa gaaaaaacat tgcattttaa tttagataac
    27421 ggtatagtaa ttaacaacga aagcataaaa cttaatagaa ttcctttaag tttagtcccg
    27481 tgaggctaag aagggtggga gcaaggatac gtatggtgcg ccataagtag attgctcatt
    27541 gacatagtat agctatcagt gtatcagtat acgctcagtc taaacattcc tcctgtcctc
    27601 ttagggcagg agttttttta tgcaaaaaag tagggggaat gaaaatgcaa gcaaaagaag
    27661 tagtagatca ggtaaccatg aagcgagcat tgactcgaat tacttatgag attatcgaga
    27721 gaaaccatag tatccaagat atcgtattgg taggtatcaa aacacgagga atctacattg
    27781 catcaagaat tgccgaacgc ctgaagcagc tagaagatat cgatattcct gtaggagaac
    27841 tggatatcac tttataccga gatgacaaaa aagaaaatcc agaagaaccg gaacttcatt
    27901 cttctgacat cccagtttcc ttagaaggaa aagaagttat tttgattgat gatgttttgt
    27961 atacaggacg aacgattcga gcagcaatgg acgcagtaat ggattttggc agaccaagaa
    28021 aaatttcatt agcagtcctg gtcgatcgtg ggcatcgaga attgccaatc cgtgcagatt
    28081 atgttgggaa gaacattcca acatctcggg cggaagagat tttagtagag atgcaagagc
    28141 ttgatggaca agatcgcatc atgattttga aagaggaaga ttagaggaga gagaaacatg
    28201 tcggagaaaa aaggatttca taatgatgac gtggtgttag atatccacga tcgcccaacc
    28261 ccaggacatt gggtaggatt aagtttacaa cacttgttca cgatgttcgg agcaaccgtg
    28321 ttagtcccca tcttagtagg tatcgatcca ggtattgcgc tagtcagttc aggactaggt
    28381 acattggtgt acttatttac tactaaagga aagatacctg cgtacttagg aagcagcttc
    28441 gctttcattg cagcgatgca gcttttgatg aaaacagatg gttatccagc gattgcacaa
    28501 ggagcagtca caactggatt agtctatttg attgttgcgc tgatcatcaa aaagatcggt
    28561 tcagcatggc tggataaaat cttgcctcca atcgtcgtcg gtccagtcat catggttatc
    28621 gggttgggac ttgcggcaaa tgcagcaaca aatgctatgt ataatcaggg cacgtatgat
    28681 ttcaagtata ttgctgtagc gctgatcaca ttaggattaa caattttctt caacatgtgg
    28741 ctaagaggtt ttctcggatt gattccgatt ttactaggta ttattagcgg atacatcgta
    28801 gccttgctgt ttggaatcgt ggatactaag ccaatcgtag acgctgcctg gtttgctgtt
    28861 cctaactttg agattccctt tgtacaatat gaaccaaagc tatatcttgg agcaatcaca
    28921 acaatggcac ccatcgcttt tgtgacgatg acagagcata tcggccatct tatggtattg
    28981 aataaactaa ctaaacgtaa tttctttgaa gaaccgggtc ttcataagac gttgacaggt
    29041 gatggattag cacaaatcgt ggcagccttt gtcggaggtc ctcctgtaac aagttacggt
    29101 gaaaacattg gtgttctggc tatcacacgt gtccatagcg tgtttgtgat cggtggagct
    29161 gcagtatttg cagtcatttt aggattcgta ggaaaattaa gcgctttgat tttaagtatc
    29221 cctggaccag tcatctcagg aatcagtttt atcttatttg gagtaattgc cgcaagtgga
    29281 atgaagattt taatcgagaa caaaatcgat tttgataaaa agaaaaatct gttgattgct
    29341 tcagttatct tagtagtagg aattggcgga ctcgtctttg aagtaggcac atttacattg
    29401 tcggcaatgg cattagcaac tgtacttggc atcattttaa atcttatctt accagaaact
    29461 tcacgaagcg aagaaaacta aggaggaaac attatgatca tcaaatcaga acgtatcagc
    29521 ttaaagcatt tattgaccgt tgaggcactt actgatcaag aagttatggg cttgatccgc
    29581 agagggcagg aattcaagca aggtgctaat tggactccgc aaaagcgcca gtactttgct
    29641 acaaacctct ttttcgaaaa tagtactcgc acacataaaa gcttcgatgt agcggagaaa
    29701 aaactagggc tagaagtgat tgaatttgaa gcaagcacaa gctctgtcca aaaaggggaa
    29761 acactatacg atacagtact gacgatgtca gcattaggcg tagacgtagc ggttatccgt
    29821 catggtgatg agaactatta tgacgaactg atacaaagta aaacaatcca gtgttcgatt
    29881 atcaatggag gagacggaag cggacagcat ccaacccaat gcttgcttga tttgatgacc
    29941 atttatgaag aatttggtca ttttgaaggt ctgaaagttg ccatcgtagg tgatatcact
    30001 cactctcgcg tagctaaatc caatatgcag atgttaaaac gtctaggcgc acaagtattc
    30061 ttctcaggtc ctagagaatg gtacgacgat gaattcgaag tgtacggaca ttatatgcca
    30121 ttggatgaac tactagatca agttgatgtc atgatgatgc ttcgggtgca acacgaacgc
    30181 catgatggaa aagaaagttt ctcaaaagaa ggctaccatc aggaacatgg gttaacagtt
    30241 gagcgtgcga agaagttgca aaaacatgca atcattatgc acccagcacc agttaaccgt
    30301 gatgtagaat tagctgattc attagtggaa ggattacagt cgcggatcgt tcagcaaatg
    30361 agcaatggag tattcgtcag aatggcaatc ttagaagcag tgctaaatgg aaaagcgtaa
    30421 ttataggagg atgaaaaaat gaaaacactc attaaaaacg gtcagatcaa tacacgaaaa
    30481 aatatgacga caccagcaga aatttggata gaagatgggc gaatcaaagc aatcggtaca
    30541 ggattctcag aagccgaatt tgatgaagtg tttgatgcaa agggacaatt gatcacacct
    30601 ggtttagtgg acgtccatgt tcatttgaga gagccaggat ttacatacaa ggagacgatc
    30661 gaagctggga ctcgctcagc tgctcgtggc ggtttcacta ctgtatgcgc gatgccgaat
    30721 ttaaatcctg tacctgatac ggcagaaaag ctgagacaag tatatgacat cattagaaaa
    30781 gatgcagttg tgaaggtatt gcagtatgcg ccaatcacag aaaatctgcg aagtgagaaa
    30841 ttagttgatc aagaagcact gattgaagaa ggtgcttttg ctttcacaaa tgacggtgta
    30901 ggtgtccaaa cagctgggac gatgtatctg gctatgaaag aagctgcaaa aaacaataaa
    30961 gccctagtag cccatacaga agatgaatct ttattatttg gcggcgttat gcacgctggc
    31021 aagaaggcag aagaacttgg tcttcccggg atcttaagcg taaccgagtc ttctcaaatt
    31081 gctagagatc tgttgctagc tgaagcaact ggtgttcatt accatgtttg ccatgtttct
    31141 actaaagaaa gtgtccgtgt aatcagagac gcaaaaaaag caggaatcca cgtaacagca
    31201 gaagtttcac cccatcatct tattttgatc gacgaagata ttcccgaaga tttcggcttt
    31261 tggaaaatga acccgccgct aagaggacgg gaggacagag aagctttgat cgaaggcttg
    31321 cttgacggta cgatcgattg catcgctaca gaccatgccc cccatggatt agaagaaaaa
    31381 agccaaagtt tcatgaaatc accatttggt atcgtaggaa gcgaaacagc gtttcaactg
    31441 atctatactc attttgtaga aactggtcga ttcacgttgg aacaagtgat caattggttg
    31501 gcagttaaac cggctgagat ttttggtttg aatgcaggaa cgctaacggt tggtgccccc
    31561 gctgatgtag cagtttttga tattacacaa acttgcacga tcgataaaga agacttttta
    31621 tcaaaagggg agaatacacc ttttatcggc tggaaagtaa aaggcgaaac acaaatgact
    31681 tttgtcaacg gaaaacttgt gtggcagaag ggagaataaa agtatggaac gtttgcttat
    31741 attagaagat ggaacagttt tcgaaggaaa ggcttttggt gcagatgcta gtgtcttcgg
    31801 agaaatcgtt tttacaacaa gtatgactgg ttatcaagaa actatcactg atcaaagttt
    31861 taacggtcaa atcattacat ttacctatcc tatggtgggt aattatgggg taaatagaga
    31921 tgattatgaa tcgatttctc caacatgcaa aggagtagta gtaaaagaac atgcacgtgt
    31981 agcttcaaac tggcgaaacc aaatgacgct agattcattt ttgaaacaaa aaggaattcc
    32041 tggaatatca ggtatcgata cgcgcgccct tactcgcaaa atccgtgaag tcggaacgat
    32101 gaaagctagt atagttgatg ctggagattc cttcgaacat gcatttgatc agctgaaagc
    32161 aaccgttcta gcaaccaatc aagtgcagca agtctcaact aacaaacctt atccaagtcc
    32221 aggaacaggt cggaaagtca ttgtcattga cttcggcttg aaacatagta ttttacgaga
    32281 attgagtaaa agaaactgta acctcacagt acttccctat aacacagatg ccgagacgat
    32341 tttagcgcta tcaccagacg gagtcatgct gacgaatgga cctggtgatc caaaagatgt
    32401 gccagaagca atcgaaatga tcaaaaaaat ccaaggaaaa gtaccgattt ttggtatttg
    32461 tctcggtcac cagttatttg ctttagccaa cggtgcagat acctataaaa tgaaattcgg
    32521 acatcgagga ttaaatcacc cggtacgaga agtagcgacc ggaagaattg attttacttc
    32581 tcaaaatcat ggctatgcag tcgatgaagc cacgattgat ccagaaaaac tcatcgtaac
    32641 tcatgtagaa gtaaatgacg gaacagtaga aggagtcaaa cacagagatt atcccgcttt
    32701 cagtgtgcag tttcatccag atgctgcccc tggtccgcat gatgcactac atttatttga
    32761 tgaatttatg gaaatgatgg acgcaggaaa ggagcagtaa acagatgccg aaacgtacag
    32821 atatcaagaa aattatggtg attggatctg gtccgatcat cattggtcaa gccgcagaat
    32881 ttgactacgc aggaacacaa gcctgccttg ccttaaaaga agaaggatat gaagtggttt
    32941 tagtaaattc taatcctgcg acgatcatga cggataaaga agtggcagat caagtatata
    33001 tcgaaccgat cacacttgaa tttgtatcaa ggattcttcg aaaagagcgt ccagatgctc
    33061 tccttccaac tttaggcgga cagacaggcc tcaatatggc aatggagctg gcagcatcag
    33121 gtattttaga agaattgaac atagaacttc taggaacaaa gctttctgcg atcgatcaag
    33181 cagaagatcg tgatctattc aaacagttga tgaaagaatt gaatcagcca atccccgagt
    33241 ctgaaatcgt caatacagta gaagaagctg tagattttgc aaataaaatc ggctatcctg
    33301 tcatcgttcg tccagccttt actttaggcg gaactggtgg aggtatgtgt gaaaatgaag
    33361 aagaattacg tatcgtatca gaaaatggat taagtctttc ccccgctacc caatgtttga
    33421 ttgaacgaag tattgcaggt tttaaagaaa ttgaatatga agtgatgaga gattcggctg
    33481 ataatgcaat cgttgtctgc aatatggaaa attttgaccc ggtgggtatc catacaggtg
    33541 attctatcgt atttgcccct agtcaaacat tgtctgacca tgaatatcaa atgcttcgtg
    33601 atgcttctct ttcgattatt cgagcactta agatcgaggg aggctgtaat gtccaactcg
    33661 ctcttgatcc acatagtttt aattattatg tgattgaagt caatccgcgt gtttctcgtt
    33721 catctgcgtt agcaagtaag gcgactggct atccgatcgc taagctagct gctaaaatag
    33781 cgcttggatt aacattagac gaaatgaaaa atcctgttac aggaacgact tttgcagaat
    33841 ttgaacctgc attagattat gttgtagcta aaattcctcg ctggccgttt gataagtttg
    33901 aaaacggaga acggctttta ggcacgcaga tgaaagcaac tggtgaagtg atggctatcg
    33961 gtcggaatat cgaagagtct ttattgaaag ctgtgcgttc tttagaaatc ggtacacacc
    34021 atatcgaact tcctgagttg gcagacatca cggacgatga cctaatccaa aaagtggttc
    34081 gagcacaaga tgatcgcctg ttttatgtag ctgaagctat tagaagaggg tacaccatag
    34141 aagaattagc ggatttgaca aaaatcgatt tattcttttt agataaactg ttgcatatcg
    34201 tagaaatcga acagcagttg aaacagcagc cgttggatga caagatattg aaaacagcga
    34261 aacaaaacgg ttttacagac aaaaaagtgg cggagctgtg ggaaataaac gaagaagccg
    34321 ttcgccagat gagaaaagaa aatgatttga ttcctgtata taagatggtt gacacttgtg
    34381 cagcggaatt cgaatcaaaa acaccttatt tttatagttc ctatgaatat gaaaatgaaa
    34441 gttcacgatc agaaaagccc tcaattctag tactgggatc tggtcctatc cgtatcggac
    34501 aaggggttga atttgactat gcgactgtgc attctgtcaa agcaatccag caggcaggat
    34561 acgaagcaat catcatgaac agcaatcctg aaacagtttc gactgacttt tctatttcgg
    34621 ataaattata ttttgagcca ttgaccttcg aagatgtgat gaatgtgatc gagctagaac
    34681 agccgatagg agtaatcgtt caatttggtg gtcagacagc aatcaactta gccgaacctt
    34741 tgactgctgc tggtgtaaaa atcttaggga caacgatcga agacttggat cgtgcggaaa
    34801 atcgtgattt attcgagcaa gcgctgaaag agttggatat tcctcaacct ttaggggata
    34861 ctgctacaag taaagaagaa gccgtggtta tcgcttcgag aattggttat ccagttcttg
    34921 ttcgtccaag ctatgtatta ggcggacgtg cgatggaaat cgttgaaaac cagcgagatt
    34981 tagaagatta catggaacat gctgtgaaag catctccaga acatccagta ctagtggacc
    35041 gttacttgct tggtggtgaa tgcgaagtgg atgcgatttg tgacggtgaa acagtactca
    35101 tcccagggat catggagcat attgagcgtg ctggtgtcca ttcaggggat tcaatggctg
    35161 tgtatccacc tcaaacatta tctgaatcga tcaagaaaac gatcgaagat tacacgatcc
    35221 gcttagcatt aggtttgaat tgtgtcggga tgatgaatat ccagtttgtc atccatgaaa
    35281 atgaggttta tgtgatcgaa gtcaatcctc gagcaagtcg gactgttcca ttcttaagta
    35341 aggtgacagg aattccaatg gcacaaattg cgacgaaagc tattttggga gagaaactaa
    35401 agacacttgg ttatgaaaac ggactttatc cagaaacaaa agaagttcat gttaaagcac
    35461 ctgtcttttc ttttacgaaa ttgcagaaag ttgatactta cttagggcct gaaatgaaat
    35521 cgactggaga agtaatggga tctgacagac aattagacaa agctttgtac aaggcattcg
    35581 aagcttctgg attacacttg cctgaatatg gagccgtatt atttacgatt gcggacgaaa
    35641 caaaagaaga agcactagaa ttagctaaac gttttacaga aattggctat agtctgatag
    35701 gaacgaaaca tacagctgct tatttcgaaa aaaatggtct ggtcgttgaa acagtagcga
    35761 aaatttctga ggaagcagca gaaaaaaatg tagtcgactt aataagagat ggtaaaacac
    35821 aagtggttgt caatacgatt gataaagatc ggggaaatgc ttctaaagac ggtttcatca
    35881 tccgaagaga agcagttgaa catggtattc cattgtttac ttctttagac acagcagatg
    35941 cgatcgttcg agtgatggaa tcccgtgctt tttcaactaa ccctatttaa cggcgagcac
    36001 atgttaagac aaaaagacga aaaaccgttg caggagggga cagatgaaac aagaaatgat
    36061 ggaaatcgtt cagcaaaaac agctggcacc aaagattttt gaaatgacat tgaaaggtga
    36121 tctagtaaaa gaaatgcagc aaccaggaca gttcttgcat attctcgtgc cgcgtgcaga
    36181 cttgctatta cgccgaccga ttagcttgaa tcatatcgac cacgaaacaa atacttgccg
    36241 tatcatttat cgggtggaag gtgaggggac tagagtcttc tctactcttt ctgccggaga
    36301 ccatttagat gtcatgggtc ctttaggcaa tggttttgat ttgtccaatc ttcatacagg
    36361 agacgaagta tttataatcg gtggaggaat cggtgtccct cctctatatg agttatcaaa
    36421 acagttagtc gaaaaaggaa ttaagccaac gcatttttta ggttttgcca cacatgaagt
    36481 cgtttattat gaaaaagaat ttaatcgtct aggagaaaca agaatcgcta ctgacgatgg
    36541 aacatacggt atgcacggaa atgtcggtaa tttattgtta ggaacgaaga cggaacctac
    36601 agctgttttt gcatgtggtt caaacgggtt attgaagaca gtggaacagc tgttttcttc
    36661 gcatcaaaat gtccagttat cattagaatc ccgcatggca tgtgggatag gggcttgcta
    36721 tgcttgcgtt tgtcatacgc cggaagatga aacaggaaca aaaagtgtga aagtttgtga
    36781 cgaaggaccg atttttaaaa taggagaggt aattatatga atgagaatcc tttagcggtc
    36841 aaacttcctg gcctagaatt aaaaaatcct atcatgccgg caagcggctg tttcggtttt
    36901 ggcaaagaat acggaaaata ctatgatttg aatcagcttg gatcgatcat ggtcaaggca
    36961 acgactccaa atgcccgctt tggcaatccc acgccaagag tggcggaaac accatctggc
    37021 atgttgaatg caattggttt gcaaaatcct ggtctagacg ttgttatgca taaacttctt
    37081 ccagaattag aggagtacaa tgagttaccc atcatcgcaa atgttgctgg tgcttgtgaa
    37141 gaagactatg tagaagtttg tagcaaaatt ggcgaagcac caaatgtgaa agcaatcgag
    37201 ctgaacattt cttgcccaaa tgtcaaacat ggcggaattg cttttggaac agattctgat
    37261 gttgcttttc aattaacaca agcagtaaag aaagtttctt ctgttcctgt ctatgtcaaa
    37321 ttgtccccaa atgtaacaga catcgttcca atcgctcagg caatcgaagc tggaggggca
    37381 gatgggttta cgatgatcaa tactcttctt ggtatgcgga ttgaccttaa gacaagaaaa
    37441 ccgattttag ccaatcaaac aggtggactc tccggaccag caatcaagcc tgtcgctatt
    37501 cgtttgattc accaagtagc aagtatatcc agccttccaa tcattgggat gggcggcgtg
    37561 cagacagtgg atgatgttct tgagatgttt atggctggag caagtgctgt agctataggg
    37621 accgcgaatt tcaccgatcc gtatatctgt cctaaactaa ttaaagagct ccctgtgcga
    37681 atgagcgaac taggcatcga atcgctggaa agattaatca aagaagtgag agaggaacgt
    37741 gaacgatgaa tcaactagac caacgaccaa ttattgcatt agatttttca actaggcaag
    37801 aggtagaaga ctttcttcga tttttcccga aagaagaaaa attgtttgtg aaaatcggaa
    37861 tggaattgtt ctaccaagaa ggacctgaaa tcgtacgtta tctaaaagag gctggtcata
    37921 atgtattttt agatctgaaa cttcacgata ttccgaatac ggtcgaaaaa gcaatgcggg
    37981 gattggcaaa gcttggtgtt gatctcacct gtgttcatgc cgctggcggt attcgtatga
    38041 tggaggcggc aatgcgagga ttagaagagg ggactccaga aggtggaaaa cgtccgctac
    38101 ttctagctat cactcagctt acttctacaa gtgaagaaga aatgcatgcc gatcaactaa
    38161 tcgaagtttc tttagaaaaa agtgtgatcc attatgctag ctgtgccaaa aaagcaggat
    38221 tagatggtgt ggtttcttct gcttgggaag tagaagcgat caaagaaatg gcaggagacg
    38281 aatttgtttg cttaactccg ggtataagac cagaaggaac agtcgcagga gatcagacaa
    38341 gagtagtcac accttctcag gcaaaaaaga tcggctcaac ttttatcgtt ataggtcgac
    38401 caatcactca gtcaactaat ccttataaag catatcaaac gattcaaaca gaatggagct
    38461 aacctaaaat gaatgttgaa caaagtatcg caaaagactt attagaaatt gaagcagtat
    38521 ttttaaatcc aagtaatcct tttacatggg caagtggaat caaaagccca atttactgtg
    38581 ataaccgaat cacgatgagc tatccaaaag tccgaaaaga gatcgcaaaa ggattagcaa
    38641 gcaaaatcaa agaagcttac cctgaagtac aagtgattgc tggaactgca acagcaggaa
    38701 tccctcatgc cgcatgggta gcggaaatac tggatttgcc aatggtctat attcgaagta
    38761 aggcgaaaga tcatgggaaa ggtaaccaaa tcgaaggacg tatatttgaa ggtcagaaga
    38821 tggttgtgat cgaggatttg atttctacag gcggaagtgt cttggaagca gctgaagcag
    38881 caaaaagaga aggtgcggat atccttggtg ttgcggctat ctttacgtat gagttgccaa
    38941 aaggaaaagc aaatttcgaa aaagccgaaa tccctcttat gacgttgacc aattattctg
    39001 tattgattga agcagcttta gaagatcgtt atatcgatga gcaagaatta actctgctaa
    39061 aagaatggaa aaaagatcct gaaaattggc agattgaagc gcgcttgatg aagatagaga
    39121 aatccgattt gtgtctccag aatcatcaat tggaatagca aaaatccaaa aactgattgc
    39181 gagaagcatc ttttcatcag gtatgaaaag aaacttctat cgtgatcagt ttttgatttt
    39241 tttaaaattc atttttctaa gactggttag cagaaagaag agaataatct cttctctttt
    39301 ttctattata tatagatttt ctctattttt tggtaaggga gctgtttcaa aaatggtaaa
    39361 gagcggaaat gttgcgaata tcagttattt aatgatatga tattcggaaa aagaaatctc
    39421 aaagggaggt aacttttatg gggttgaatg aatttatttt aggattgaac aatttagaga
    39481 cgatcacaag ggcacctgga ttttttaaat atacagagca tacagttgca gcacattcgt
    39541 atcgtgtagc atctattgcg caagttctag gagacattga agaagcgaat ggacaaccga
    39601 tcgattggaa actgctctat gaaaaatcat tgaatcatga ctacacagag cgttttattg
    39661 gtgatatcaa aacgccggtg aaatacgcaa gcaaagagtt aagagggatg ctgcaaacag
    39721 ttgaagaaaa gatgacggac gaattcatca accaagagat tccagctgag ttccaagaag
    39781 tttatcgccg tcggttgttc gaaggaaaag atgaaaccgt agaaggagaa ctattagcca
    39841 tttcagataa ggttgacctt ctttatgaat cttttgatga aatcatcaaa aataacccgg
    39901 aaaaagttta taaggagatg ttcattgagg cagtgactac gattaaaagc tttgaacatc
    39961 ggccttctgt tcagtatttc tttaaagaga tatttccgga actattgaac caggaatttt
    40021 atggaaaaga ggcgttttta gaaaccgtct caacgattgt aaagaaagaa gaaaaataaa
    40081 aatgatatga gatgcagggg gaagtatagt tgaaaaagaa tatggatgtc gaatggggct
    40141 attctggaga caaaggtccg gaaaattggg cgagtttatg tgattggttt gcgagaggtg
    40201 cggaatttcc attgcagtcg cctattcatt tgaccaaggg ggaagaaacc aaaatagaag
    40261 gagacacgct ttcctttcat tatcaaaagc aacggtttac tgacaaggaa ttcaaaaaca
    40321 cgattcattt agtacctttt gaccaactca gttatgtaga gttcaagaaa gaacgctatt
    40381 atttgacaga tattcatttc cacttgcctt ctgaacatat catcaacgga aaacaggaag
    40441 atattgaatt tcattttgtt catatgaaca gcaaaaaaga gaatctagtt gtgggtgtca
    40501 tgtaccagct aatggtacag gaaggttggc tgtataatca agaaaatggg gaaagttgga
    40561 acttagaaaa acatgagcat tgggtaaatc cggatgtgtt ctttccggag caaaaaagcc
    40621 atttccatta tattggctca ttgacaactc cgcctaccag cgggccaatc caatggtttg
    40681 taatggatca tgtaggaaaa ctgaaccaag agtttcttct ccccttcgat ggtcagtatg
    40741 ctcgaccgaa caaccgacca atccagccct tgaatggaag agaaatttat tatttcgagg
    40801 agtttgagca atgaatattc agcagatgaa atacgtggtc gctgtagcaa acaatggtag
    40861 tttcagagaa gcggcaaaga aaatgtttgt ctcccagcca agtctttcag ctggtatcaa
    40921 agaattagaa accgagttag ggatcacgct gtttactcga acgaatcgag gggcatattt
    40981 aacagaagaa gggcaagact tcttaaatcg tgccgagaaa atattggtcc agcttgatac
    41041 attagaaact cactatctaa cgagtgaaaa gagagacaat ttttcgatcg ctgcccagca
    41101 ttatgatttc ttaggtcctt tgaccgcaca gctactcgag gaattcaaag acgagatcaa
    41161 acaatttcgg atcatggaga cgacaacagc gagagtgatc gaagaagtca aagagcagca
    41221 cagtgagatc ggcattattt atatgaatga gcataatcaa gctggtatca gaagatattt
    41281 acaacaagga gagctaagtg gccgcagcat tggaaaattc aaaacgcata tttttttaag
    41341 gcaaggacat ccattagctg aacacgcaga gatcaccaaa gaagaattgt tatcttatcc
    41401 tcaagttcgt tttacacagg atggaaataa tttcccgtat ttttatgaag accttatcga
    41461 gatccctgat caagaaacag tgatttatac gagtgatcga ggtactttga tgaatatcgt
    41521 tcttgaaact gatgcttacg cttcaggatc tggtattgtg attggaggga ttcgcgagca
    41581 cttgcggtta atccctttgg cagaaggcga actaaatgaa ttttatatca tttattcggc
    41641 gaaacgtccg ctcagtgaaa tcgctcaaag atttatcaaa gagcttactc ggttactgga
    41701 ccagcagagc taagaaaagc agatgagaac gctgtttatt gaacaccgtt gatcaaaaaa
    41761 gaggggttgt gacagaattt tgtcacaact cctctttttg tagcaaacaa tttataacta
    41821 attgctgttg tgggaaagta cgcaaatatg attctagaaa cactcattga ttacctgttt
    41881 tgtttcatag ctgtaatttt ttcttcttct ggtgtcacga taatcgtctt ttggttttca
    41941 taaataacat aaccaggctt ggcgccattt ggttttcgga tatgctttac ctgtacaaga
    42001 tcaaccggca cttgggcaga gtatcggtat tttgaaaaat aggcagctaa ctcggcagct
    42061 tctgtaattg tttcatctga gggcttgtca cttttaatga tgacgtgcga accgggtata
    42121 ttttttgcat gaagccaata gtcggttttt ttagctgtct tcattgtcag ctggtcattt
    42181 tgaagattgt ttttaccaac aagaatgagc gttccgtcag aagaaaagta ttgatcaggc
    42241 tgagatggtt tcttacgttt ttgttttttt aagttcttct ttttcaggta tccttctgaa
    42301 gtcaattctt ctttaattgc ttcgatatcc attgggccgg caatttccag ttgagataag
    42361 acagattcaa gatattggat ttcatctttg gcttcttgga tttgttcgcc aatcagctta
    42421 acagcgttct tcagcttttg gtatcgatgg aaatatttct gtgcattttg atttggtgtc
    42481 aatgccggat ctagtgctat tttgattggc cgatcttctt cataataatt aggtaaaaca
    42541 acttcttttg cgccgcgtgg tacttgtgtc agaaaagtag taagtagttc gccatttcta
    42601 cggtaattct cagcgttttc ggattctttg agtgtttgtt cacgtttttt caatttattt
    42661 ttatttcgtt ttagttcatt ttcgattttt cgaatcagtt ctccgccttg ttgttttgcc
    42721 cgatctttct ctgctttttc gtgataatag ctatctaata attgactgag tgttggataa
    42781 gctgttacag cagatgcctg ttcggacaat acctgataag caatcggtgt gaaaaaatct
    42841 ttattttccg tttcgtaaaa agtaggaata ggctgatggc tgatggctga gaaaaattcg
    42901 tcccatacaa ccatcttttc attaggacgt tccagtaatc ttttggtcag ctcttgagcg
    42961 atatcgaaac cgattccctg aaactgacgt tggattgctt ttggatctag ttcagtttgg
    43021 gaaagccgct gaaagatttt ttctttctcg gagctaaatg ggttcaagac ctcttgtttt
    43081 ggcggagcaa catattctac accaggaagc aaagaacgat aagtgttttg agagcttcca
    43141 atgtgtttga tcgcatccag tatttttcca gtttctcgat taactaagac gatcgtacta
    43201 tgacgtccca ttaattcaac gatcaatatg atattttgta gatcacccaa ctcgtttcgt
    43261 ttagcaaagt ggaaatggat cactcgatca ttttcgattt gttcgatgtc ttctaaaata
    43321 gcgccatcca aatattttcg aagcatcatc acgaagttag gcggattatc aggattctga
    43381 taatcgattt gggtaatctg tacacgagca taacttgggt gcgcagataa taataatcga
    43441 tggtttttgc cgcgtgaacg aatcaccagc acgatttcat tttcataagg ctgatggatt
    43501 ttcgagatcc ggccagatag taacgtttca cgcaattcgt tgaccatcgc gtgggtaaat
    43561 acaccatcaa aggacatggt tcattcctca tttcttttca taagtatatt cattataacg
    43621 aaaaaaagaa gaaaaaccaa tttggccgaa tagtaaagca aaagtctaga aaaatcttgg
    43681 actaattaca taaaaaacag tgtttctatc gttgaaatcc aaatttttag gt
//
LOCUS       DAJSBL010000020        40891 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.020, whole genome shotgun sequence.
ACCESSION   DAJSBL010000020 DAJSBL010000000
VERSION     DAJSBL010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 40891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40891
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.020"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            144..412
                     /locus_tag="ONT85_001270"
                     /pseudo
     CDS             144..412
                     /locus_tag="ONT85_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
     gene            1084..4485
                     /locus_tag="ONT85_001271"
     CDS             1084..4485
                     /locus_tag="ONT85_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HCR2378257.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTGLTTGEDGKLAIDGLAPGAYQLVETQAPIGYELDATPIEFEIERSQTAVVELTKE
                     NRLTPGGVVLTKIDDQSGEILQGAVFELQNREGETLQTGLTTGEDGKLAIDGLAPGAY
                     QLVETQAPTGYELDATPIEFEIERSQTAVVELTKENRLTPGGVVLTKIDDQSGEVLQG
                     AVFELQDANGTVLQSGLTTGADGKLAIDGLAPGVYQLVETQAPTGYELDATPIEFEIE
                     RSQTAVVELTKENRLTPGGVVLTKIDDQSGEVLQGAVFELQDANGTVLQSGLTTGADG
                     KLAIDGLAPGAYQLVETQAPTGYELDATPVTFKIEKEQEVAIFLTKENRKVADSSDIR
                     GNNPTSSGNKHLPKTGETIISQFILSILGVLLVFISIKFRKKRNI"
     gene            complement(4909..5079)
                     /locus_tag="ONT85_001272"
     CDS             complement(4909..5079)
                     /locus_tag="ONT85_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR2378258.1"
                     /translation="MPRLTNYFLSCTNPKEIEFLVTDMNATYFQLTKHVLPNAKLVID
                     RLLGFSTPFKEA"
     gene            complement(5273..5452)
                     /locus_tag="ONT85_001273"
     CDS             complement(5273..5452)
                     /locus_tag="ONT85_001273"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2378259.1"
                     /translation="MPMVIRLKKQRYTCKNCRSHWNAQSYFIRPRHSISNHVRHKITS
                     LLTEKVSLFFISKSC"
     gene            5839..6951
                     /locus_tag="ONT85_001274"
     CDS             5839..6951
                     /locus_tag="ONT85_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HCR2378260.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            complement(7029..7322)
                     /locus_tag="ONT85_001275"
     CDS             complement(7029..7322)
                     /locus_tag="ONT85_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HCR2378261.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            7490..7804
                     /locus_tag="ONT85_001276"
     CDS             7490..7804
                     /locus_tag="ONT85_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HCR2378262.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            7825..9804
                     /locus_tag="ONT85_001277"
     CDS             7825..9804
                     /locus_tag="ONT85_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HCR2378263.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            9817..10143
                     /locus_tag="ONT85_001278"
     CDS             9817..10143
                     /locus_tag="ONT85_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HCR2378264.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            10159..10677
                     /locus_tag="ONT85_001279"
     CDS             10159..10677
                     /locus_tag="ONT85_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2378265.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            10677..12029
                     /gene="celB"
                     /locus_tag="ONT85_001280"
     CDS             10677..12029
                     /gene="celB"
                     /locus_tag="ONT85_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HCR2378266.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(12113..13618)
                     /locus_tag="ONT85_001281"
     CDS             complement(12113..13618)
                     /locus_tag="ONT85_001281"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HCR2378267.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(13710..14300)
                     /locus_tag="ONT85_001282"
     CDS             complement(13710..14300)
                     /locus_tag="ONT85_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HCR2378268.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            14479..16125
                     /locus_tag="ONT85_001283"
     CDS             14479..16125
                     /locus_tag="ONT85_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HCR2378269.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            16174..16893
                     /locus_tag="ONT85_001284"
     CDS             16174..16893
                     /locus_tag="ONT85_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HCR2378270.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            17372..18319
                     /locus_tag="ONT85_001285"
     CDS             17372..18319
                     /locus_tag="ONT85_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCR2378271.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            18331..19251
                     /locus_tag="ONT85_001286"
     CDS             18331..19251
                     /locus_tag="ONT85_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCR2378272.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            19292..20749
                     /locus_tag="ONT85_001287"
     CDS             19292..20749
                     /locus_tag="ONT85_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR2378273.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            21094..21708
                     /locus_tag="ONT85_001288"
     CDS             21094..21708
                     /locus_tag="ONT85_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HCR2378274.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            21701..23425
                     /locus_tag="ONT85_001289"
     CDS             21701..23425
                     /locus_tag="ONT85_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HCR2378275.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELRELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            23422..24873
                     /locus_tag="ONT85_001290"
     CDS             23422..24873
                     /locus_tag="ONT85_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR2378276.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
     gene            complement(25346..27499)
                     /locus_tag="ONT85_001291"
     CDS             complement(25346..27499)
                     /locus_tag="ONT85_001291"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HCR2378277.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDREYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
     gene            complement(27501..28547)
                     /locus_tag="ONT85_001292"
     CDS             complement(27501..28547)
                     /locus_tag="ONT85_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR2378278.1"
                     /translation="MTQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            28697..29986
                     /locus_tag="ONT85_001293"
     CDS             28697..29986
                     /locus_tag="ONT85_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HCR2378279.1"
                     /translation="MVYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDACRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            29999..32701
                     /locus_tag="ONT85_001294"
     CDS             29999..32701
                     /locus_tag="ONT85_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HCR2378280.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            32686..33543
                     /locus_tag="ONT85_001295"
     CDS             32686..33543
                     /locus_tag="ONT85_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HCR2378281.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(33614..34513)
                     /locus_tag="ONT85_001296"
     CDS             complement(33614..34513)
                     /locus_tag="ONT85_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HCR2378282.1"
                     /translation="MVQLVCNCTERSTLVKITITLPMNHPETTIRDLLEKEWLVPRKV
                     RHFLRIRKNVWINQQPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDE
                     HLIIVDKPAGMKTHPNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKN
                     PFVLPILGRMLEQKMIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSA
                     LTHVTVNKVLSEQTQVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHAL
                     ELHMIHPFTQEKIVGKALPGLWE"
     gene            34682..36853
                     /locus_tag="ONT85_001297"
     CDS             34682..36853
                     /locus_tag="ONT85_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HCR2378283.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPAVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            36921..37262
                     /locus_tag="ONT85_001298"
     CDS             36921..37262
                     /locus_tag="ONT85_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HCR2378284.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(37538..39292)
                     /locus_tag="ONT85_001299"
     CDS             complement(37538..39292)
                     /locus_tag="ONT85_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR2378285.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQCISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(39303..40139)
                     /locus_tag="ONT85_001300"
     CDS             complement(39303..40139)
                     /locus_tag="ONT85_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HCR2378286.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            complement(40141..40824)
                     /locus_tag="ONT85_001301"
     CDS             complement(40141..40824)
                     /locus_tag="ONT85_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HCR2378287.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLMPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
BASE COUNT        13263 a         7147 c         8476 g        12005 t
ORIGIN      
        1 ggatagtaat tttagagggg aatcaatgaa ggaagaatag aagggaaaaa tagttgcaaa
       61 tacgccagtt agcaaaaata gcaatacatc ttcaagcttt aaaattcgag agaatcatct
      121 tggtagtgga atcaactaca acaaaaggat taaaccaatt aatagataga attaccaatg
      181 gaaaaataga aaaggtagtt attctataca aaagttgttt aattcgcttc ggttatgaac
      241 ttagtgaaac cctttgcaat atatggtaca gaatatagaa attattgaca ttacagaaaa
      301 tgatgaagat ttaatacaga ttgtcactat ttgtagatac cttttacaag gaaaccgagc
      361 caataaagct aagaagatga ttaaggagtt gatggaagac gatacaagtt aagaaagtga
      421 aactacgccc aaataaagag caaaaagagt aattggacgt ggaaaatgca agaaatgaac
      481 catcgattcg gtggacagtt tattagtaat gacggtttga gaacatccat gacgagaatg
      541 aaggaacatc ctaaatgcgt ttagttgaac caagtgccaa ataatatggc caaataagcg
      601 atcagttatc gatgaatccc aagtattcta aactatatat tcattttgat gctaagcatt
      661 gatatatttt tttgggattg atgaaggtta accaatacag ggattcactg atgaaattct
      721 agagtctgac cttggtatca acgatttagt gattgtttct aatggaaaag tataaaagta
      781 tataagtaca ttaacaaaat aactattagg gttacgctcc agcttgtgca aggcactggc
      841 aggaggaatc gtgaaatcat aattatggtt aagccacacg gacaactaaa agtcatacaa
      901 agctatcgaa tcaatcaaca gaagacaatc ggcaatcatt agctttgttt acagcttaca
      961 ttgcaacgct tgagttgaac cgaaaatacg agggaggtaa cgcagccatt ttgagttggg
     1021 gaatcttttt agaaaattat cgggtacctt gttgtttagt caaaattata agggggagta
     1081 atagtgagga acaaatttca taaactgtta agtatgtttc tagttatgct tattgtgatc
     1141 aatcaatttg ggaacataac ggttctagct gagtctttag cagaacagcc agagcgttcg
     1201 ctgacgatcg aagttgatca agaggaggct gctttgggtg agcagttcca gttgcgagtc
     1261 agaggaacac cagacctgct aaatgcattg gtcattgata agagtgagga tttgattgaa
     1321 ggggaagtga agcaactcaa tgattacgaa cgtctgatta cttattcttc ccagcattca
     1381 ggtaaatacg aattgaccgc aagaagcggt gatattcaat caaatacggt ctcaatagaa
     1441 atcagtgtac cagaagaaag cacaagtgag tcttttaatg taccagaaga aagcacaagt
     1501 gaatctttta atataccaga agaaagctta agtgaatctt ttaatgaagc cagatcatct
     1561 gagccatcta taacgcagga atctcagatt gaaagacaag aggagctctt gaattatgac
     1621 atcggcatct ctgctgagcg ccccactttg ccgagcaggc atacacaaac gttaaaggga
     1681 tacattcaag gaggcgctgg cgatattctt gaagaatatg gggagatggt cgtacgagtt
     1741 cctagagcat cgattggctt aattgagttc attagagtgg aggtcgctga tgattcacct
     1801 ttaaagccac tggcatttta ccaagaaggc acagacgttg tttttccatt tacctttgat
     1861 tatgaaggat taaatgaagc cgctcagtcg tatacattca gtatcacgtt tgaagttgct
     1921 ttctttcaat caggcggtac tgagccaaat aatgactttc aagtgagtct tacttatgct
     1981 gagcgagcag caaacacgga attttcaatt acgcctattc cttcatcaag acccattttt
     2041 tcgaaattat actatggctc tagagaaatc gacagccaag gaaatcttgg gaatctgctg
     2101 attgacgggc aagacgtgac caacagtaat aaatttattt tgcaggtaaa ttatgaagga
     2161 tcacgaaaag acggtgtagt ggttaccgat gtactaccgc aaggaacaac tttggcaccg
     2221 aatccgacta catggagttc cactggaaat agagaaccta tttcaggtgg ggttcggatc
     2281 gcagaagtaa cgaatccagg tgaagcaaac gttagaactc attatgtttc cgaggaattt
     2341 gtggacaaag tatcttttaa taaggaaacg aatacacttg ttgtggactt tggtaatcta
     2401 gcagagggtc aatcgtattt tattgaatat gctcttgaag tgaccgatgt cgacttgttc
     2461 aatgaaggaa gacaagagga tgggttttct cggaatcgag cgagtatgac ctacaataat
     2521 caggtaatga gccgagacta ccgcttgaga atccctgaaa gtaatcttcc ggcaatctct
     2581 ttaagaaaaa gtgtggacaa aaatgttatc aatctcaatg aaaatgaact aacgtatact
     2641 ctgcatgtct cgatgttttc tggaacaaga aatacgcttg ttattgaaga tgttttggaa
     2701 gaaggcatgg aatttgtgtc atttctatca gatattccct ctttcattga tgccgaagtc
     2761 aataatggaa tagttcgttt cagtgcgaat aatacgattg gtggtccgac aagttttacc
     2821 ctctctttta aggtcaatgt agaggcgtat gcggtcggcg atcaaatttt aaactttgca
     2881 acgcttgtga atagtgacaa tcgattgaat acgagtactg taagaacaag gaaaatcgat
     2941 gggcgtatta aagtaattaa aactgatgag aatggccaaa ggctggcagg agcaaaattt
     3001 gaaattttat caagcgattc acaagtgata caagaaggta taacggcaga aaacggagaa
     3061 tttttaagtg aaccgcttcc tgtaggtagc tatcaagtcc gagaaacaga agcaccggaa
     3121 gggtatcttt tagatgcgac ggtacatgat gtttttataa atgaatactc catagaagaa
     3181 gtgacgttaa ccattgaaaa catccttgat acgggtggtg ttgaactaat taaacatgct
     3241 gaaggcagtg gtgaagtcct tcaaggagct gtttttgagt tacagaaccg agaaggagaa
     3301 acgctacaaa ccggtttaac tacaggagaa gatggaaaac tggcaattga cggtttagca
     3361 ccaggagcat atcagctggt agaaactcaa gcgccaatag ggtatgaatt agatgcgaca
     3421 ccaatcgagt ttgagatcga aagaagtcag acagcggtgg tagaattgac gaaagaaaac
     3481 cgacttacac caggcggtgt cgtgttgaca aagatcgatg accaaagcgg ggaaatcctg
     3541 caaggagctg tttttgagtt acagaaccga gaaggagaaa cgctacaaac cggtctaact
     3601 acaggagaag atggaaaact ggcaattgac ggtttagcac caggagcata tcagctggta
     3661 gaaactcaag cgccaacggg gtatgaatta gatgcgacac cgatcgagtt tgagatcgaa
     3721 agaagtcaga cagcggtggt agaattgacg aaagaaaacc gacttacacc aggcggtgtc
     3781 gtgttgacaa agatcgatga ccaaagcggg gaagtcctgc aaggagctgt ttttgagtta
     3841 caagatgcca atggaaccgt tctgcagtca ggtctaacca caggagcaga tggaaaactg
     3901 gcgattgacg gcttagcacc aggagtgtat cagctggtag aaacccaagc gccaacgggg
     3961 tatgaattgg atgcgacacc aatcgagttt gagatcgaaa gaagtcagac agcggtggta
     4021 gaattgacga aagaaaatcg acttacacca ggcggtgtcg tgttgacaaa gatcgatgac
     4081 caaagcggag aagtcctgca aggagctgtt tttgagttac aagatgccaa tggaaccgtt
     4141 ctgcagtcag gtctaaccac aggagcagat ggaaaactgg cgatagatgg cttagcacca
     4201 ggagcatatc agctggtaga aactcaagcg ccaacggggt atgaattgga tgcgacacca
     4261 gtaactttca agatcgaaaa agaacaagaa gtggcgatct ttttaacaaa agagaataga
     4321 aaagttgctg atagctcaga tataagagga aacaatccaa caagcagtgg aaataagcat
     4381 cttccaaaaa ctggagaaac catcattagc cagtttatat tgtcaatatt gggtgtgcta
     4441 cttgttttca tcagtataaa atttagaaag aaacgaaata tatgagctaa aataaaaaat
     4501 tgaataagca tagtttttaa aatcaactag tacccatcaa ttgaatggaa actaccgatt
     4561 ttagaagggg tatacaaaca aatattagta tcggagtaga gaaggttgaa cccaaccagt
     4621 aaataagtaa agacaatttt gtgctgcgtt gagaagtgaa aaataaacct ttgtagtaat
     4681 actagttaat aaaaaatata caaaaacact gcgaaaatca ttcttgagtt tcgcagtgtt
     4741 ttttgcctaa tgactcaagt ttattgggta atactttgat tttctttcta caaataatca
     4801 gcgatcagtt gtaatttttt ctctagtttt aatggctctt tgtcaataag gagaaaaaca
     4861 agtcgggtct tttgtctcgg aaagcttcaa ccaactcgtg aataaaggtt acgcctcctt
     4921 gaaaggcgtg gaaaaaccaa gtaatcgatc aatcaccaat ttcgcatttg gtagaacatg
     4981 tttggtgagc tgaaagtagg tggcgttcat gtctgtaact aagaattcta tttctttagg
     5041 attagtgcag ctaaggaaat agtttgttaa tcgaggaatt ttttgtgtag gtaaattttc
     5101 tcgattaatt tttctgtctc gccatccgcg gaaataaaac tcagtttatc ctcaacggaa
     5161 acgtgagaac gaaattcaca accctcaaca ctcaagacag aattcttttg gaagactttg
     5221 gtaaatagct tctcaactct ttcaatgtgc gaataacggt tgtcaaagat acctaacaac
     5281 ttttcgaaat aaaaaataac gacacttttt cagtcagtaa agaagtgatt ttatgtctga
     5341 catgatttga aatcgagtgc cttggacgga taaaataact ttgagcgttc caatggcttc
     5401 gacagttttt acaggtatag cgctgctttt ttaggcgtat aaccattggc atatgattat
     5461 attattcaaa gtggacaatg gtttccttct tcttgttttt caccaccatt aatttcctat
     5521 ttccatctac cacagcagaa ccacaatttc tacagacaca tggagcaggg aagagaactg
     5581 cagcaacgat caatgttttt ttcttctgta aggtttcgta agagacctct gtaatcatca
     5641 aatttttgtc tattgatctc agaatttttt tgatagaacc attcataaag catatcgttc
     5701 tctcagttgt ttattttgtg gtgatttaat catacaagaa aacgatatgt ttttttataa
     5761 aaacatgaat aggaaaaata gttgacaaga aaatgacaac gccatatgat gttccatagt
     5821 atttacaacc tgtcatagat gactgtaaaa aataaaagta aaaagaaagg acgacaacag
     5881 gtggattttt atcagtctct acaactagat ccgtttattt taaaacaaaa aatacgagaa
     5941 gcagcttcta aaaaagaaaa gtgcatgtat atttgttcat tgtttttacg atcattattc
     6001 atcgtattgt ttgccatttg ttttatcatt ataattacaa cattatttga atcgaagcat
     6061 aaaccatacg ctgtggtttt attctgtatg ttgatgagta ttcgttttgt tgactttggt
     6121 tataagattt cccattcgat cataggttta gcaatcgtca tgttctcact attagtagca
     6181 ccgtatgtcc agctgatcaa atggtcagtt atgggcatgt tgattcattt tattttacta
     6241 agcagcatat taatggcaac cgctagtgat cccaaaatgg ggaatgcttc tttgtacggc
     6301 ttttcatatc tctttattgt ttattcgtta cctaaagatt cgttgaacaa gaatttcttt
     6361 acacaaacaa gtagcttatt atttttgttt ttttgttggt tttcagttct cctttacaga
     6421 aaacatcgag aaaagaacaa agacaaaagc ttgtttaaag agattttttt gaaaggcatg
     6481 tattcgcagg aaaaaatttg gatgctcatc tatgcatttg gcattagtct tttgatcgtt
     6541 gcaggagagt atgtcccttt tcagcgattg atgtgggcag ggtttgcatt ttcttctatt
     6601 gtatctagct atggattgat gtcgattggt ttcaaagaac gagcagtaga ccgaatcatt
     6661 ggctcattga ttggatgtgt tttatttatt ggtatcagcc aatttattcc ttttaactgg
     6721 gtcggtatat taggtggtct agctttaggt gtttgttcta cctatagata taaaacagtt
     6781 tttaattctt ttggtgcttt ggcaatcact gccagtttat tcggggttcc aggagcagta
     6841 acgatccgca tttttgagaa tatccttggg gtatgtttag gaatcatgta tattggggtg
     6901 actgaaatac ttatccgaaa aataagagaa aaacacggtt tgaatcatta gatgagatcg
     6961 aataaaaaat aaggaaaaag tgaagataca catggatgat aaattgctat cattccatgt
     7021 gtatcttttt acggtaaatc agtgaaacgt tcgactttca tttttaattt atacttttta
     7081 cggagttcgt ttatttgagc catcatttcg gatttgtgat gcttttcgat tgcttcttca
     7141 tctttccaac ggtcgatcag taaaagagat tctggatctt ctagtggaaa gtaatagtca
     7201 tatttctcgt ttccttcttc tgcacgaatc gctttgacaa tgtttctttc gaccatttct
     7261 tctgcaaatt ttctggcttg tccatttgta cttgtataca aaatattcac tgtgatactc
     7321 actaaatccc ctcctgaaaa aattctatca ctagtatatc acggtgccag aaattattat
     7381 tcaagatatg atacacattt tttccgcaat gctacggaaa tatatatttt gaatagaaaa
     7441 cgcttacaag atatgcttgg tttataaaaa taaaaggagt gattgattta tgaaaaaagc
     7501 attgattatt tgtgcggcag gaatgtcatc ctcgatgatg gcctcaaaga caacggattt
     7561 ctttaagcaa aaaagagaag aggtactcgt agatgcagtt tcagctacag aaggcgataa
     7621 tatgattaag aatagtgatt ttgatttatt tctgatcagc ccgcaaacga cgatgttttt
     7681 ggataaattc aaaaaattag gagaatctgt tggaaagcct gttgttagta tccctttcca
     7741 agcgtatgtc ccaatcccta caggcatcca gaagttagca gaggtgatcg aagagaacat
     7801 atgatggaat aggagtggat gataatgaaa cagaaggaaa aattgctgtt gagatatttg
     7861 attgatcaca agaaggagta tgtcaccagt caacgtttag cttctgaatt gttattatct
     7921 gatcggacaa tccgtaatta cttgcagcgg atcaaagagt tagtagaaaa aaacggaggt
     7981 aagatcattg ctaaaccagg ttatggctac caactgcata ttttacaaag actgacattt
     8041 gacttgtttt tgagtcggca agaaatagta cctggatatg ctagagaacc gcaaggattt
     8101 tatgagtccg aagatcgaca aaagtatata ttgaaccaat tgcttttgga agatgccatt
     8161 ctttttatgg atgatttggc agaagagctt tatatcagtc gttccagttt gacaaaggac
     8221 atgcaagaaa tcaaggagcg attgattcca tattcactaa aaatcgtttc taagcatggt
     8281 caagggacat ggatcgaagg ggaagaaaga aatcgacggc attttatcat ggatactttt
     8341 tttggaaacc attatggaaa ttcattgaaa gaatatttag ggcaaagccg ttttttccca
     8401 gacatcagtt ttgaagagct agtcattatc attctcgatg aaacaagaga agctaagtta
     8461 aaagtttcgg attttatcat acagaatctg actttacact tggctttggg gatcaaacgg
     8521 ctccgagaag ggtttgaaat caaggaatta ggcatcgaac aagaagtttc taagcgagta
     8581 gagtatcaag tggcgcagcg gatcgttcaa cgtattgagg taatcgcagg tgtatcgttt
     8641 cccgaagaag aaattgctta tttgacatta catctcatgg ctaagtcgaa tcacaaagaa
     8701 aatcaaaatg atcaggaact atcaaaggaa ttagcgaaag ttctgaaaag attgtctcag
     8761 gaatttcatt tttcattagt agaggatctc cagcttttca atggactact cgatcatttg
     8821 tcaccgatga tgattcgatt gagccgaggt attcgcattg agaacccatt gactgaggag
     8881 ataaagaaag aaaatcccaa agtctttgac gcagtaaaaa ggcatttcag caacatgccg
     8941 gctttgaaaa actataccat caatgaagat gaatgggcct atttggcttt gcatctcatg
     9001 gcggcattgg aaaaagaaag agctgcgcat aaactacatg cattgattat ctgtgcaacg
     9061 ggttacggca gtgcccaact attaaagaat cgcgtagtca gtgaattcgg taaaaatatt
     9121 acagtggtaa gtgtaaaagg ctattatgag atcaatgaaa caactttaaa aggaatcgac
     9181 ctgattattt cttctattga tctttcgaca atgtttttca aaattccagt tttacatgtc
     9241 agtatttttt tgaatgaaga tgatgttcgt aaaatacgaa aggtagttgg agaatctata
     9301 ccctatcatt cctcagatag accgatacca gcattttctc tacagcaaaa aaagcagatt
     9361 tacaccgaac agttgtctga aaaatttttc aagacttata cgtacgaacc gaaaaaggaa
     9421 gaaattcttc atgatttgct gcaaaagctg agcataaatg aagaagaagg atatataaga
     9481 gaaatgcgaa agcagatcca gcaacgtgaa aatatgggac aaatcatatt cagtgataca
     9541 gtggcagtcc cacatcccgc tgttccagtt ggaatcgaaa caaaggtagc cgttgcattg
     9601 attccttctg ggatgcagtg ggatcaatat caagaaattc attttgtttt tttagtttcg
     9661 ccttcttatg tcgaaaatga aggaattacg gtcgtgacta aagcaatcgt taggttagtt
     9721 gaccagctaa aagtacagca ggaaatctta gctgaaccta tttttgagaa tttcagcagt
     9781 aaatttatca aactgatcga ataaggagga aaacagatgg ctgaaaaaat aaacagtgaa
     9841 acactacaag tgacttcttt tgatatcatt ttccatagcg gaaatgcacg gacactgata
     9901 catgaagcat ttcgctcgat gcgtaaagag atgtttgaag aagtagaaga gaagttgcga
     9961 caagcaaatg aagagattct tcatgcgcat cagtcacaaa ctcaacttct gaaagaatat
    10021 gccagcggga caaaaataga aatggaaatt atcatggtcc atgcacaaga tcatctgatg
    10081 acgacgatga cattattgga agttgcacag gaaatgaatt atttgtatca aaaaaataaa
    10141 tagaaaggaa ggatcattgt gcaggataca gtaaaaagga aaatcgaatt agaaaaaaag
    10201 caacttagtt tgaaaaacat gtatttcaat cgttatctaa tcgtacgtta cctgactgcc
    10261 tttttctttt tcatgaacat gcaatggatg attctgcttc tatcagctaa aagtttagga
    10321 agtagtttac cgatgattct gattttagct attttgcctg cagtaggaga acaagtaaag
    10381 ctttatcgaa aacaccaaac gaacgttcca tggacaaagt ggtatttttt gttccagagt
    10441 gtctttaatt tattagtgat tccagtgttg tttacctcag gatttacatt cttgtatcct
    10501 tttatggcaa ataacaatgg aggacagtta tttgttttca ttcttgttgt aagcggtatt
    10561 tttgccagtg catttataca gtatcgttta aagaaaattt cactcaacca agaccaacag
    10621 tatatccgta ttaaacaata tgaaaaagcg ttatatttag gaaaggagaa taactaatgt
    10681 ttgatttttt gcagaaatac ctgatgggac ctatggggaa aattgcgcaa cttaaaatcg
    10741 ttcgggctgt catggcagcg gggatggctt ctattccttt cacgattgta ggctcgatgt
    10801 ttttggtttt aaatattttg ccattaccgt tcccattttt agaaggattt tttaatgcaa
    10861 cttttttcaa agttagtgac ttgtacatga tcgtcaatac aatgacgatg gggattttat
    10921 ctatttactt tgccattgtg tttgcttatg aattaacgtc aattgaacga gatgaacaaa
    10981 aattaaatgt taatccgctc acgggggcat tactatctgt ttttgcattc tttatgtgta
    11041 tccctgaatt gatcatgact gatggtaaaa tcagcttgat tgcttctatc aatgagacag
    11101 aaactgtagt aaatggtatt aggatgggct cttttgtcga acgtttagga acttcaggaa
    11161 tctttacagc tattattatg tcgattattg cggtcgaatt gtattgtatg tgtgtcaaga
    11221 gaaattgggt cataaaaatg ccagatgttg ttccgccagg agtttctcgt tctttcactg
    11281 ctctgatccc aacatttgtc attgcgtttg tggtgatgat catcaatgga ttattagttg
    11341 cactaggaac ggatatcttc aaaatgattg ctatcccatt tgggtttgtt acgcagttga
    11401 cgaatacatg gcttggtgtc atggtgattt atttcttgat ccatgcattg tggatcgtag
    11461 ggatccatgg agcgaatatc atcacttctt tcttgacccc gatcgtatta gctaatatgg
    11521 cagctaacgc acaaggcgca aattatccgt tggcaggaga attcaacaat tcttatgtaa
    11581 ccgttggtgg atcaggtgcg actttaggat tgatcatctt tattgcattc ctcgcgaaat
    11641 ctgatcagct gaaagtgtta ggaaaagcct cacttgtacc aggattattc aatatcaatg
    11701 aaccaatcat tttcgggatg ccaatcgtct acaatcccta tttagcggtt ccgtttttct
    11761 tagctccgat ggcttctgct acactcgctt attttgccat tcggctggaa atcgtcaaac
    11821 cgatgcttgc acagatgccg tggccttcac cagtcgggat cggagcattt gttggtagtg
    11881 gtggtgattg gaaagctgct gtgttagcag tcatttgtgc aatcatcgca ttcattatct
    11941 ggttcccatt tatcaaattc tatgataata aattattaaa tgaagaaaga gaaaaggcag
    12001 cagaattagc tagtgaaaag ttaaaatgat ttctaagaaa aaaggaactg tgacaaaaaa
    12061 tcacagtccc tttttttctt agaaatggga aaccactagt atataaatac cattattctt
    12121 caatcaaccg cttcacgatt gcataatccc cttcataagg ttcatctaca ccattgattt
    12181 tttgataggg atccacgcct tttcccgcaa taacgactga atcttgcgga gcgctttctg
    12241 ctaaagcgcg tcgaatcgct tcaggacgat ccatttcata atgaacgatc aaatctggat
    12301 tagtaattgc ttcattgatt tcttccgcga tttttttcgg atcttcaaat gctgggtcat
    12361 ctgccgtcaa aaaggcgatg tcagctgttt ctgatagcac ttttccaaaa tcatgtcgac
    12421 gggagattgc tttgtttcct gggctgccta atacaacaat tacacggcca tctggatgtt
    12481 catttttggc aaatttcagc aatgttttca agcttagata attatgtgca tagtccacat
    12541 agacatgcgc tccattggat tggatcaatt gatccattcg gcctggtaca cgagcttctt
    12601 ttaatccgtc ttgtgcatct tgactggagg cacctgttag tgctgcggca atgatcgcac
    12661 tggctgcatt ttcttggttg aatcctccag ccagcaaaat atcataagca gtatcgcaga
    12721 tagctaagcg atcttcatga gaagataaag taaaaccgtg agtaccttta tctgaacgga
    12781 tcacttgata attgctttct tccgaacgac cataagtgat cgttggaatc tggaatagat
    12841 cggctgtttc cctaagtaaa tcaaaatagt cactttcatg atttaaaaca actgtttttg
    12901 aattcaagat caattgtcgc ttgcaataga aatagtcatc aaatgtcgga tgttcgatcg
    12961 gactgatatg gtcaggcgag atattcaaga agattccgac atctaaagtc aagccgtata
    13021 cgcgttgtgt tttgtaagct tgtgaagaaa cttccatcac tagatgagtc ataccatttt
    13081 caactgcttc tgacatcata cgatataaat ctaatgactc aggcgttgtc aaatgggatt
    13141 tgaaatatgt tttgccatct aacgttgtat tcatagtaga aagcaatgct gtttttttat
    13201 ttgttgtgtg atccaagatt gcttttgtaa agtacgcagc agttgtttta ccttttgttc
    13261 cagtgaatcc gatcactttc aatttatttt gcggatagtc gtaaaaagcc atagcaagaa
    13321 gcgccattgc tttacggata tccgttacga tgattcctac tgctgaattt tcgtatggtt
    13381 gttctgatac ataatattga ataccctgtt cgattgctga atccaaatat tcttctttga
    13441 aattcattcc tttacagaaa aacaaagtag aagcatcagc ttttcttgaa tcataactta
    13501 actgaagaaa ttcagctttt tctggtacat caagatgcca gcctcttgtt gaaacaaatt
    13561 cttttaatag attttctttt aatagtaaat gacggatgtc ttctaaagag aagctcattg
    13621 attgatccct cgctttattc aatttcttgg tttattatag cacactcttt ttaatatatc
    13681 gtttgtttcc gctatgacaa aatccagctt tattcaaatt ttctcgacac aggggacaag
    13741 cggatgagta ttttcataaa caatgaactg actgtaatag ataggagaac gaatagtgtc
    13801 tccaacaatc caaaaacata ataattcaaa aagacgataa gactattggt aagtaaaagt
    13861 ccttgcggca ttggaagccg aaaaaatttg tggaaaataa tcggcggtat cgttataccg
    13921 ccatttgagg ccccgactgt gtacaatgtg taaaccccaa gactgaacaa aagacttcct
    13981 gaaatcagtg aaaagaaatg tgaggagatc atttccactt ttggaacaat ctctaaaaca
    14041 acaggaaaaa gcagacttcc cgctacagtc ttaaaaaata ctgattttcc taataggaca
    14101 gcggctagaa gcagcatcat aagattgatg ctccaaacag taactgagcg ttggatagaa
    14161 aaggccgttt caactaaatg tccgatggcg cccactccgg cggaagctat atcgtgaggg
    14221 agcaagaacc aattcaatcc aactgtgata aagaaaagac ctaaaaatat aacgggtagt
    14281 ttttttattt cttgtgtcat tgatatctcc tataaatgtt agccataaaa ggctgtatat
    14341 ttctttactt taccaaaaaa acgaaaccga attcaaaaaa acaattgttc ttataaaagt
    14401 tatataaaat cgattgccag tttctgtttt accaattaaa atgatagtaa tgttttgtag
    14461 aaagttggaa tctgaccgat ggataatcaa agaaaaccag cattagatca attaacaccg
    14521 caagaaaaaa tggcacgagg ctctgcttgg atgaccgcca gtaatatcat ttcaagattg
    14581 ctaggcgctg tctatatcat tccctggtat gcttggatgg gcgaaaatgc taaggctgcc
    14641 aatggattat tcaatatggg atacaatata tatgcattgt ttctactgat ctctacagca
    14701 ggaatacctg ctgcgattgc aaaacaaact gcacgctata attcattgaa tgaatatggc
    14761 acttcacgac gattatttat ccgtgcatta cagatgatgg gcgggttagg cctgttgttt
    14821 gctttgttca tgtacatcgc ctctccttgg ctagctcatg cttctggtgg cggagaagaa
    14881 ctgattccaa cgatgcgttc tttgagtatc gctgtattgg tttttccttg tatgagtgtg
    14941 atacgagggt atttgcaagg caatcaagaa atgatgcctt atgcgctgtc gcagattgta
    15001 gaacaggtgg cacgtatttt ttatatgcta ttggctacat tcatcatcat gaaagtcatg
    15061 gatggtgact atgtgacggc ggtcacgcaa tcaactttcg cggcatttat cggtatgcta
    15121 gcaagtattc ttgtcctttt gtattttttg aagaaacatc aagcgtatac atcggcgttt
    15181 attcactaca gcgaaaacaa aataaatatt gcaacgaagg aattgttgtt agataccgtt
    15241 aaagaagcga ttccgtttat tattgtgggt tcaggggtaa cgatcttcaa attagtcgat
    15301 cagttcactt ttatgaagat catgtctgca tccaccgagt attccaatgc acagttactg
    15361 gatctttttt cgatttttag tgccaatcca gataagttga cgatggtagt tattgcattg
    15421 gcaacatcga tcgcgtctac tgggttaccg ttgattacag aagctgttac gttgaaagat
    15481 cgagtaggtc tggcaaaatt gacaagcgga aatttacagt tgttttcatt ttttatgttt
    15541 ccggcgacat ttggtgttat gttacttgcc tatccgctaa atacattatt ttatacaccg
    15601 gattctctag gaagtaatgt actgatccaa gcaagttttg taggactctt cctaggacta
    15661 tatatgctag tttccaacat gctgcaagga atgtttgaaa acaaggcggc aattaattat
    15721 ttggttattg ggtttctagt gaaactcctt cttcaatacc cagccattcg tttatttgaa
    15781 gtatacggcc ctttattggc gacaatgatc ggatttgctg tgtcttgtac cttgatctta
    15841 aagagaatcc atgaagtagc ccgctttaaa ccaagttttg tctggcgcag gacattgctg
    15901 atctttattc ttaccttgat tatgcttttg gcttcgtgga tcacaaagat gatttttggt
    15961 gcttttttaa acgaagatag caaattccag tctctattgt tgattcttgt agtagcaggt
    16021 ataggggctt tagtgtatgg ttatttagct ttgaaaatcc gcttggcaga aagactacta
    16081 ggaccaggaa tagcgcgttt acgacataaa ttaaaaataa aataagtgaa tcagaagagc
    16141 tagattgatg ctcttttgaa atgggggaaa cgcatgcgtt tagataagtt tttagcagat
    16201 atgggacttg gcagccgcaa agaagtcaaa ggattaatca aaaaaggaac gatcgaagtc
    16261 aatggacaag tggtgaaaag cgataaagcg caagtaaatg aattcgaaga tgcggttact
    16321 tatttaggag aaatcattac gtatcaaaaa gatttttact acattttgca taaacctgca
    16381 ggagtcattt ctgctactca ggataatcat gatcaaaccg taatcgattt attggaagac
    16441 caggattatc gggaagatct ttttccagtc ggccgattag ataaagacac agagggcctc
    16501 ttgattttaa cgaatgacgg ggtgtttgcc catcagttac tatcacctaa aaaacatgtg
    16561 gagaaagaat atctagctga ggttagcggt ataatgacag aagaagatat ccgtttattt
    16621 gcagaaggac tgattattga tggcgatgag cagacattgc cggcaaaact ccaaattgat
    16681 caagtagatc aagagacaga aacttcaatt atccgtctga ttttgcatga aggaaagttt
    16741 caccaagtta agcgaatggt caaagcagta ggaaaagaag tcttatattt aaaaagaatc
    16801 cgtatgggaa aattttggct gcctaaagat ttgaataaag ggcaatatcg acccatgacc
    16861 aaagaagagc tagaacaagt gagagaaaaa taattgaatg gatagctaaa agaataagca
    16921 gagtagttgt agcgccatct gaaaagcaga cttgtccgtc tgactttcgg atggcttttt
    16981 tgtgaaattt cggcttattt ccgtagaagc tacttctgga acaccgtttg ttcggaataa
    17041 gggactgtga caagactttt gttacagtcc ccattgttcc ttgtgactca gagttagata
    17101 gtaaggacac gatctcccta atactcaggc caaaacgctg aattcacaac ctctgataaa
    17161 cggtgttcaa tcagctgacc ctcggtatta gctcaaaaaa cggcaaaata tgaggaacta
    17221 ttttcccgtt tttttctcta atactcaggc caaaacgctg atttcacaac ctctcctata
    17281 ctttgtttca ttttccctat actttgttta tttattctca ttattgaaag cgctataatt
    17341 acgttgcgaa tgaatcgaaa ggggttttta gatgaaaaaa cgaaaggggt tcaatcattt
    17401 ttttatgaat ctatggcgtt ataaagcact tgtcctgatg gctgtaccgg gtatgctttg
    17461 gatgattttt ttcttttaca tacctgtaat ggcgaatgtc gtagcattca aaaattttca
    17521 tatttcagca gatgggttct tggctagctt gagggaaagt ccttgggtgg gttgggaaaa
    17581 tttcaaattt ttattttctt cggatcaagc atttctcatc actaaaaata caattttata
    17641 taatgtagca tttatccttt tgaatctgct gatctcagtc gtatttgcaa tcattatgag
    17701 cgagttgaga aataaacgac tcgttaaagt ttatcaaacg atgtccttac taccttattt
    17761 tctatcttgg gtcattatca gttactttgt ttacgctttt ttaagtcctg acaaaggaat
    17821 attcaatcag tggatcactg gacatggagg agaagctgtc aattggtata atgaaccgaa
    17881 atattggccg tttatcttgg tgttcattgg tacatggaaa gggattggtt ataacagcat
    17941 tatttacttt gcatctgtta tgggaatcga tccgacttat tatgaagctg ctatggttga
    18001 tggagctagc aaatggcagc agatcaaaca tgtgacgata ccacaactta ttccactaat
    18061 gacgatttta acgattttag ccgtaggaaa cattttccga gctgattttg gcctgtttta
    18121 taacattcct aggaattccg gtgcattata tgatgtgact caagttttgg atacgtatat
    18181 ctacaatggc ctgacatcca caggagattt tggtatgacg gctgctgcag gactttacca
    18241 atcagttgtc ggctttgtct tgttgatgat cacaaatacg atcgcccgac gttttgatag
    18301 cgattctgca ttattctaag gaggaaaaaa atgaaaaaaa agaaagtgga gagagtccaa
    18361 gtccgctctt tcaataagac ggctaatttt ttctctaata taatgattgc actctttgct
    18421 atttcctgta ttttgccatt tatatttgtt atcgccatct cttttaccga tgagtccata
    18481 ttgtcgactc acgggtacag tttttggcca gaaaagttca gtatatttgg ttatacgtac
    18541 ttgttcgacc agatgcagga taaaattttc caagcgcttt tcgtcactgt acttgttact
    18601 gttttaggta cactgatcaa tagcacggct acttctttat atgcttatgc gatttcacgg
    18661 tcaaactttc cattccgtcg tttttttacc gttttttgtt tgattacgat gttgttctca
    18721 ccagggatgg tggccaatta tcttgttatg acaaatcttc ttcagttaaa agatacgatt
    18781 tgggcattga tcttgccaat ggctgtcagt cccttcaata ttattgtgat gcgcacattc
    18841 tttaaacgtt cggtctctga ttcgattatt gaatcggcac ggatcgatgg agctagcgaa
    18901 ctgcgaatat ttgttcagat cgttttaccg ttagctgttc caggtattgc gacaatcagt
    18961 ttatttgccg cattgggtta ctggaatgac tggttcaatg ctttactata catccaagat
    19021 gataagctag ttccactaca gtatcttttg atgaaaatcc aaagtaatat tcaatatctg
    19081 acacaaaatt caggagcagg aagccaattg acaggcggat tagcctcagt tccaggagaa
    19141 tctgccagaa tggcgatcgt agtcatctcg acgttgccta ttgcgatcag ctatccattt
    19201 ttccagaagc attttgtcaa aggtctaaca atcggcggag taaaagaata aagaaacaaa
    19261 aatataaatc ataaaattag gaggaagaaa aatgaagtta tggaagaaac tagcctttac
    19321 aggtgtttca gcgttgttgt tgggaacatt agcagcatgc ggaggttctg gttctaaaga
    19381 ccaggcagaa gctagcaatt cggacacttt gcagatgtat caaatcggag ataaaccaga
    19441 taatttcgat caattgatgg aagtagcaaa caaacgaatc gaaaaagaga tcggtgtaaa
    19501 agtcaatatc aattacattg gctggggcga ttacgaaaag aaaatgaacg tcatcatttc
    19561 ttccggggaa aattatgata tcgcctttgc gaacaactat gtttccaatg cccaaaaagg
    19621 agcctttgct gatttgactg aattagcgcc taagtacgct gaaaaagctt ataatgattt
    19681 agatgaagca tatattaaag gaaatctggt caatggaaaa ttatatgctt tcccagtcaa
    19741 tggaaatgtc tttgctcaac aagtacttac ttttaacaaa gctttactag ataaatacga
    19801 tctttcaatt gatggaatcc aatcttacgc tgatgcagaa aaagtcttga aggaattcca
    19861 tgaaaaagaa ccaaacacgg cagcttttgc cattggacaa ggcttcaaag tccaagggaa
    19921 ctttgattat cctttaggga atacgttacc atttgctatt gatctggatg gagatgattc
    19981 gaaaatcatc aaccaatacg ataatgaaac gtatatcgga ctgttacgta caatgcatag
    20041 ctggtacaaa caaggactca ttccaagtga cgctgcaacg agcaataagg attatccact
    20101 agaaggaaat acttggctaa tgcgtggaga aacacaagga ccgtatgatt atggcgatac
    20161 aattttgaca aatgcagcaa aacaagaact tgtctcaaaa gcaattactg ttccactaaa
    20221 atctacagct caagcacaaa tggcaaattt tgtcgtatca aatacttcaa aaaataaaga
    20281 aaaatcagta gaacttctag ggctgcttaa cagtgatcca gaattattga atgggcttgt
    20341 ttgggggatc gagggagaag catgggaaaa agtcgatggt tcagatgata aaatcaaatt
    20401 gctagatggt tatcagccaa atacacatat gtctgcttgg aatacaggaa acaacaagat
    20461 tctatataca caagagtcta ttacggatga catgattgcc aaacgtgatc aatcgattgc
    20521 tgatgcagaa acttcaccga ttttaggatt cagcttcaac actgacagtg tcaaaacaga
    20581 attaagtaat atttctaatg tcatgaatca atatttagat ggattgaata cagggactgt
    20641 tgatccagat gaaacattac cgaaattgaa agacgctttg gacaaggctg gctatgataa
    20701 ggtattgaaa gaaatgcaaa aacaatatga tgagttccga caagaataat aatgaataaa
    20761 gacaaagggg ttgtgacaaa agacgtgtca caacctcttg ttccgaataa acggtgttca
    20821 aaagccagtt tctcggtatt aagctaaata tatgtaaaaa tgtggaaagc catattcaca
    20881 aaatttttct tactactcga aactaagcgg ctatttcaca aactcttggt aaacggtgtt
    20941 caatcagcta accctcggta ttcgctcaca aaacgacaaa atatgaggaa ctatttttcc
    21001 gtttgtttct ctaataatca ggtcaaggtg ctgattgccc aacctttttt tgtataatga
    21061 gaaagtataa acttaatgtt tggaggagaa gatatgttag ggaaggtttt ggaagagtta
    21121 tttatccgga tctgggtcat tataaaacta accttgtatt tctggatata tacatttgcc
    21181 ggcgggatca tctttggatt aggtgcggca tggaaaacag tcaatgaact attttatttg
    21241 tacggattcg agtataaaga aatcacaata aaaagaggct ggaatatata taaaagaaat
    21301 tttttaagag gcaatctgct atttagttta tttttatctg gtactgcttt attgagttac
    21361 aatttatatt tatctgttca aatcaaaggg ctcatttttt tagctattga ttttatttta
    21421 ctttttgcct tgctctgcct atttgctact tatcaataca gtatgatttt agatagtgaa
    21481 tatacgatct ctgttcctaa tctgctaaag ctaagtttta tttcggtttt ttccagcttt
    21541 tcgtcttttc taaaaataat tattggtagc ggtataattt taggcgtgac ttggcaattc
    21601 aaaggattga tcctgtttgg tgtcattggt cttttgacgg tttggaatgg tacgatgaca
    21661 acacattggc gtgaagaatt agataaacag ttggaaagtt atgaataagt ttcggattct
    21721 attttcaaaa ggaaccttac taaatcgtct gttgcggaaa tacgcggtta tcatgatcgg
    21781 tgtaacgatg actgctaccg ttatttttag tatccatact tgggaacaaa atcaaaaaca
    21841 ggcagaaaac atgacaagtg atgccgttca aagcacaagc agaatgctga atgataaaac
    21901 aaccttgagt aggatcatca aaaaccagct tgtagggaat tccgaaaaga tcgaaaatgt
    21961 gacgacctac ttaacaaaac ccatagatca gtatctgatg tatgtatacg aacagcaaaa
    22021 cagtaccgat gaattagttt ctttcccaaa tcaaatcaaa gatctctacg caaattatga
    22081 agaactaagt gcgatatata tcgtgttgaa tcaactaccg gagtattatg aatcttctag
    22141 agaaaacaaa ggtggggaaa taaaaaaggg gacaccaaaa ttactagacg ccttttatat
    22201 caagttgcct atcacacaag gaggagctga gtcaggaagt atttttattg gttttcaaaa
    22261 gaaagaacta gatatgattt tagaaaatct aacgtcattc aatggactct ctttatacat
    22321 gatttctgga acgacaaatc gactatacac attccatgat aataaaatag ctaaagaaac
    22381 gtttgaaaga caagaaatga tcatcacaga gagtctgaag aaaaattcac aattgccgat
    22441 tgacgatctg gaaaaaaaca attttgttca acaccaagaa ctgtctgatg attatcgtat
    22501 attagctgta ctagataaaa aaagtgtaaa aatcgaaacg ggaaaaaatc ttgccccgct
    22561 gatcattggt atcattattt tagacaatat actgcttatc tttctttata aaacattcaa
    22621 acgatattca cagcaagtat ctatcgtcat gcaggccatg gatcaaacag cagcagggaa
    22681 tttagagacg cggatcgaca taacaaacac ggaatatgaa ctgagggaac tttcaatcgg
    22741 tatcaacgaa atgttggata gcatcaatca gtttgtagaa gacatctaca aattagaaat
    22801 caaacagcaa gacgcacata tgcgggcgct tcaagcacag atcaatccac actttttata
    22861 taatacatta gaatatatcc gaatgtatgc aatcagcgaa ggaagtgaag aattagcaga
    22921 tgttgtgtat gcgttttccg ctttgcttcg aaacaatacg aatcaagaga aaacgatcac
    22981 tttaaaagaa gaactggact tttgcgaaaa atatgtctat ttgtatcaaa tgcgttatcc
    23041 taatcgagta gcctatcatt ttatgatcga tcctgatttg gaaaagatcg aagtacctaa
    23101 atttgtgatc cagccattag ttgaaaatta ttttaaacac ggtattgatt ttactcgatt
    23161 cgataatgcg ctaagtgtca aagtcttgca ggaaggcaaa cgggtaagga tcatcatcaa
    23221 agataatgga aaagggatga ctgaaaaacg gctaaaacag attgaagaaa aactgtctca
    23281 tcctaaagtg gaacttcacg gttctatcgg tctgcaaaac gtgaatgaac gtttacgtgc
    23341 cagcttcggc agcagttatt atatgtcgct tgagaataat gagacaggtg gactgaccgt
    23401 ttctattaca ttcaaggagg gatgagcgtg tatcaagtgt tattggtaga tgatgaatac
    23461 atgattttga atgggttaaa aaagataatt gattggcaaa gtttaggctt tcaaatagta
    23521 gcaacagcag agaatgccaa agaaggacta gctgtacttg aacagcgaca gattgattta
    23581 gtagtaacgg atgtgacaat gccagaaata aatggactgg aatttattga agcggctcaa
    23641 aaagaacgtc ataattttga atttatgatt ttatcaggat accaagaatt cgattatttg
    23701 aaaggaggta tgcaattagg cgccgtgaat tatttgatga aacccgtgaa taagttcgaa
    23761 ttggttgaaa gcctcaagaa aatcaaaaca cggttagatc agcaaaatga acaaaaaaat
    23821 caacaagaaa tctatcaaga actgttattc agtcaatggt tgaatgaaga attggatgaa
    23881 ataagtgaag aagaaataat aggacaaata agtgagaagc agcataggat agtattgatt
    23941 caactggcaa gaactcatga tactcttatc aatagatggt tgcaagaacg gcaacaaatc
    24001 ttttactatc aacgaaatta cggagaccgt gtattgatca cgttgttgtt ggaggcagat
    24061 gaagttgatg gcttctgttg ttttgttcaa gaaaactttc ctgatcagga atggctgatc
    24121 agtatcgggg aagagacaga ttctttggac aaaatccctg aaacattcca acaagcgaaa
    24181 gacaatctac agttgcatca attttatgga gataaagacg agcatgtttt ttacgcagta
    24241 caagcagaaa caaaagatca aaccatcgat ttttcaagtt tcagacgcgt gctgcaaaat
    24301 aaacaattgg aagtcgctca aaaaatgatc actgattttt ttgaacagtt tcagttagca
    24361 gccatgatgc cggaagatat acgatattcc agttttttac tgtttatgga gattcaacgg
    24421 gaattgattg atctggaaga tgaggaatat ttacaaggaa tcgaaaaaat ccatcaggca
    24481 aaaacggtgc aagaacttca gcagctactt ctgtcattca tcaaaaagca tcagagacag
    24541 aaaaagtaca gtgacaatgt ggaaaaagta atcgtaatcc tgcaccagca ttatcaagaa
    24601 ccacttacac tcaaagaagt gtcagaaaac ctgcatttaa atgtcatgta tctaggacag
    24661 ctgttcaaaa aagaaacgaa aaaaagtttt tcagcttatt tgaaccattt acgtatggaa
    24721 aaagcgaaac aactgctttt gcacagtaat caaaacatca atgagatagc aagtgagatt
    24781 ggctacaaca acaccacgta tttctctaaa ttattcaaga aaatcgtcgg cagatcccct
    24841 aaagagtacc gagaaaacag tggagaatca taaaagaaaa agccaatgta atgaaataac
    24901 ttataaaaaa ttgtctagaa atctaatttt gggctgttct tattacttga ggcaaggcag
    24961 ctttttcatg acctctcgat aaacggagtt caaaagccag tttctcggta ttaagccaaa
    25021 tttatgcaaa aatgtggaaa gccattttca caaaattttt cttaatactc gaaactaaac
    25081 agctttttca cgacctctcc actaaacggt gttcaatcag ctgaccctcg gtattagctc
    25141 aaaaaatggc aaaatatgag gaactatttt gccatttgtc tctctaatac tcaggtcaga
    25201 acgctgattt cacaacctct caataaacgg tgttcaaaag cttgcatctg cggtaataag
    25261 accaaccaaa cgaaaaatat gaggatctat ttttgcttgg ctgttcttat tacttgatgc
    25321 aggacagctt tttcacaacc tcttgttaat tatagtttga taaggaaaaa ggcaaatctt
    25381 ttgcttgata tttcttaggg tgagggatga tccctaattg gtaagagatc gtgccgcctt
    25441 tcagcaaatc ttgatgggta aaatataatt tcgtatattc tctgtccatc cgtttgattt
    25501 tatggataaa ttgttgctgc ggccgattag gcgttgcttc aatcgtcaat tcctttccat
    25561 ttgacaaaag caaaacagcc ttgtcaatta atggcatacc gataacatat tcaccggttc
    25621 caggcgtgac aggataaaat cccagactat tgaagatata ccaagcggcc atacttccat
    25681 tgtcctcatc tccagggtaa cccattggag aatcgtcgaa ggtctgagtc atcaactgtt
    25741 taagcagtgg ctgtgccatt tcaggttgtc ctatataact gaacaagtaa ggatagtgaa
    25801 aacttggctg gtttgaaata gctaattgtc caaattctat cgcggccatc tcactcattt
    25861 cgtggatctc aaaaccatat cctcctgggt caaacgttgg gggctgattg cacagctcaa
    25921 tgagtttttc tttatatctc tcactgccac catattttcc gatcagtcct gaaaaatcgt
    25981 gataaacagc aaagctgctt tgccaagcac ttccttctgc ataatcttgt ccccaacgat
    26041 tagctgaaaa tggttctctg aacttgccat ctttatcttt tgaacgcata aatcctgact
    26101 catagtcaaa gatattttga tagttcaatg attgctgctg atatttcttc gtcatttcct
    26161 cttcttctag taccttggca acttggctaa tacaaaaatc gctataagca tagtctaatg
    26221 tgtgattgac tgattcgtgg tagtgattag gaacatagcc atatgtcaag taatcttctg
    26281 ttccttggcg tccataattt ggatgtttgc tttgaaccgt agcacctttt tccattgccg
    26341 ttagaaattc aggcatcaag tctcgtcgga tatcttttac agcagcatct gcaatgaccg
    26401 catcgatcaa tgtcccaggc ataagtccac gttcatcagg agacagccat tttggcaaat
    26461 agccactttc tctatagctg ttcaaaaaac cttccagcat ttcttcgtat ttttctacag
    26521 caatcaaaga ataaagagga taaaccgttt tatacgtatc ccaaaaccca ttatttgtat
    26581 agagaacacc aggttttata gatcgactag tcgtatcata gtggattttt tgcccactag
    26641 gatcaatttc ataaaatgtt tgtggaaata aaaacacccg atataaatta tgataaaacg
    26701 tccgcaattg ttgttgatca tggtgtgtga gctgtatacg atcaaaatag gactgccatt
    26761 gttcttgact gtgagcaagg taagtctctt tgctttggtt ttgttctagt tccaggttat
    26821 aagctgcttg ttccgagctg ataaaagatg tcccaaatcg gatagtttgt ttttttgtct
    26881 gttcaaaata caacgggatc gagtcgctat ttttgttgga aacagctaac tcatcaattg
    26941 ttaaaggata ttcaaatctc aatataaaat aaaaagaaaa atcaggatct tctgaaccag
    27001 caaaatgtgt gatttttcct tccactgtgt aaggatctat aacaaagagc tgatggcgtc
    27061 ccggaaagga taaaagtaaa ccgctgtcat ttttgctgta atcaatagat aagattccgc
    27121 catacatgct aggtatcaat gtagaacaga tgccataacg taattgacag atcgacagat
    27181 gagtcgggca aaatatactt tcttctgggc ggtaagaact ttgagcatgg aaaagggtgt
    27241 tctcctgtag ttttccattg atcggcgtaa acagcatatg actgaaatca cccatccaag
    27301 gacttggttg atgggtaaga cggtagcctt gaaaaacgcg gtcatcagga tgaaaccacc
    27361 agctgccgtt ttgatccgta gtttgcggtg caaaataatt catcccaaaa ggaacgccag
    27421 tataaggctg gcagttccca ttagagaaat ttggctgatt cgctgttgca tgtctagtat
    27481 ctatttgacc gatattcatt ttatttctcc tttgttgatt gtcttttgat gaatgaaact
    27541 ggaaatttac tatcggatgg tcgatctttt gtctctaacc aagtgaccaa agcatctccc
    27601 gcaagcttac ctaactgttc aaaatcttgt gcgatggttg tcagtggcgg gtcaacaaga
    27661 gctgctgctt ggatatcatc aaatccgata attgaaaaat cttcagggat atgataacca
    27721 tgctgtctca agagattcat ggtctggata gcgacaacat cgttttcaca aacaaaagca
    27781 gttacttcat acttagtgat ataagaaagc aattcatcta atgtcgcgct tttgtcatcc
    27841 agtgaagtgt ggaaagtcaa accagctttg tccaatgctt caaggtaacc aaggtaacgc
    27901 tgtcgaacgg attgtggatg agtctgcgta ccaaacaaat aagcgatttt tgaatgtccc
    27961 tgttcgatca aataagaggc agcaagcgca ccgccttgga aattatcgga aagaacggta
    28021 ggaaaaggta actcatagat ttttttatct aatatgacga cagggaattc tttcaatgaa
    28081 agctcaaaaa gtaagtcaag atgttgttct gtatcaagag cataataaat caatccgtca
    28141 aattcattga ttagctcttt tgctgtttta ttttgtagaa agtcaagcgt cgtcatcatg
    28201 acatcaatct gtttatcttg catcacaggg ttcaatccct tgctaaaatc accaacagac
    28261 aaatcagcga catatggaag aagaaacaga atccgtcgtg tttttgtagg agaagcaaga
    28321 atctgttccg gagaagcgga tttaacgaaa ctgcctttcc cgcgaacgcg ataaataagt
    28381 cctaattgct ctaattcagt caatgcacgt ttggaagtga ttctgcttac tttataggta
    28441 cgtgaaagtt ctttttcagt tggtagttgg ctgtctacag gtaattcacc agttaaaata
    28501 gccgtttgca aatcattaaa tattgtctgg tacaatggtt gagtcatata aacacctctt
    28561 aatgatatat catattcaaa taataccata atggatgaaa aagtaaataa aaagatattt
    28621 tttgaaagcg tagacataat tggctgtatg aagtataatc tttaaagata tatcaaatag
    28681 aaagaaggtt ttattagtgg tttataaaga tataccaaaa tcggtgcaga aatttatgga
    28741 agatatcaca caaaaatgtg gggatatcca tgcggactgg gcaaagaatt ttacagcagc
    28801 atttgccaat actctgttga caacagtcaa aaagcaaaat gacggaacca cctttttatt
    28861 gacgggagac attccagcga tgtggttaag agactctact gcacaagttc gtccttattt
    28921 agtgattgca aaagaagatg aggaattagc tgacatgatc agcgggttag tcaaaaagca
    28981 atttttttat atcaatatcg atccatatgc gaatgccttc aacgaagaag ctaacggtgc
    29041 tggccaccaa acagatcata caaaaatgaa cgattggatc tgggaaagaa aatatgaaat
    29101 tgattcttta tgctacccaa tacagttggc ctatcttctt tacaaaaata caggaaaaac
    29161 tgatcaattc aatcaatctt ttattgaagg tatcaaaaaa atcttggacg tgttcaaaac
    29221 agagcaagat catgaacact ctccttatac atttgtacga gacacgacaa gaaaagaaga
    29281 tacgctgatc aatgacgggc gaggtgcaaa aacagctttt acaggaatga catggtctgg
    29341 tttccgtcct agcgatgatg cttgccgtta tggatatctg gtaccatcca atatgtttgc
    29401 ggtggtcgtg ttaggttatg tggaagaaat ttttcaaacg atactcgatg ataaagaaat
    29461 tgtacagcaa acacagaaat tgaagaaaga gatccaagag gggattcaga atcacggacg
    29521 aacggttaat caaaaagggc aaacgattta tgcatatgaa gtagatggaa tgggaaacgc
    29581 cacagtcatg gatgacagta atgtgcccaa tctgatcgcc gcaccttact tgggatattg
    29641 ttctgaacaa gaggaagatt atctgcaaac aagaagaaca ttgttgagta aagaaaatcc
    29701 atatttctat gaaggaaagt acgcaaaagg aattggatcc tcccatacgc cagaaaaata
    29761 cgtttggccg attgcattag ctatggaagg aatgacaacg tcagacaaat ctgaaaaaga
    29821 gcggatattg aatttacttg tagcaactga cgcaggaaca caccttatgc acgaaggatt
    29881 cgatgtgaac aatcctgaaa attatacgag agaatggttt tcttgggcaa atatgatgtt
    29941 ttgcgaatta gtcatggatt actttgatat tagagtagaa aagtaggagg tatcgtaaat
    30001 gaaaaagaaa gtatatatca tttctcatag ccactgggat cgtgaatggt acatggctta
    30061 cgaacagcat cacatgcgac tgattgaatt gatagacgat ctactagagc tatttgaagt
    30121 tgatccgtcc ttcaacagtt ttcatttgga tgggcaaacg attattttag atgactatct
    30181 tcaagtaagg ccagaaaaaa gacaggcaat ccaacaagca atcaatgaag gaaaattgag
    30241 aattggacca ttttatatct tgcaagatga ttttcttatc agctcggaat cgaacgtgag
    30301 aaatatgctg attggcatgg aagaaagccg taaatgggga acccctgtca tgttaggcta
    30361 ttttcccgat acatttggaa atatgggaca gacgcctcaa ttaatgaaac aagctggtat
    30421 ttcagcagca gcgtttggcc gtggtgtaaa accaattggc tttgataatc aagttttgga
    30481 agcagaaaac tattcttccc aatactcaga gatgtggtgg aaaggccctg atcaaacagc
    30541 aatctttggt ctgctatttg ccaattggta tagcaacggg aatgagattc cagtagaaaa
    30601 agaagcagct cttgcctttt ggaagcaaaa attagcggat gcagaacaat atgcctctac
    30661 gaaccactta ttgatgatga atggtgttga tcatcaaccg gtacaaaaag atatatcaaa
    30721 agcaatccat ctcgcaaatg aactgtttcc agattacgag tttattcatt ctaattttac
    30781 tgactatctg gaagcagttc aaaaagatgt gccagaagac ttaggctcag tagaaggaga
    30841 attgacaagt caagaaacgg atggctggta tactttagcc aatacagcat ctgctcgggt
    30901 ttacttaaaa caatggaata ccaaggtcca gcgacagcta gaaaatatta cagaaccact
    30961 tgcgacgatg gcttatgaag ttagtggaaa ctatccccat gatcaattag attatgcttg
    31021 gaagacatta atgcaaaatc atcctcacga cagtatttgc ggttgttcag tcgattctgt
    31081 ccatcgggaa atgatacctc gttttgaaaa agcagatgaa gttggaaagt atcttgcaca
    31141 agattcctta gaacagctga ccgcagcaat cgatacgacc ggattcccaa aagacagctt
    31201 tccatttgtc attgtaaata ctgcaggaat ggataaaact ggtgaagcag aaataaccat
    31261 cgaattagaa agaaaaagat tcgctgaagg aatacctgaa caactatatc aagagttaga
    31321 aaatttgcct aaacgtaagt atcatgtaga aacaaaaagt ggggccacta taccagctat
    31381 tttatctgaa gaaactgtcc aatttggtta tgatttgccg aaagaccgtt tccgtgtgcc
    31441 atatatggca agaatgatca aagtgacttt accattagaa aacatgccag cattttcttg
    31501 ggaaacattc gcgcttgtag aaggggaagc gaaagcagaa gaaaaggaaa caatgattca
    31561 tcagagtgga cgaatcatcg aaaacggccc tctacatttg acgattgaaa aaaatggtac
    31621 cattacaatg gaagatcgca aaaacaaacg aaagttgaat aatctgcata tttttgaaga
    31681 tatcggcgat ataggaaatg agtatatctt taaacaacct ttctgtgatc agccaatcct
    31741 atccagtaac aaagaaaact ctgaagtaaa agtattagta gatacaccgg aaatagcaaa
    31801 aataagtatt ctacaggaaa tggaaatccc tgtttctgca gacgagcgtt tagaaaaaga
    31861 acagcagatg gttgtggaat ttcgttatcg aaaagcagaa cgatccaaag aaaaacgaat
    31921 ccttcaaata aaaacaatca tgacaatcag aaaagatagt aaaaaaatcg actttgaaac
    31981 gactctcgac aatcagatga aagatcatcg cttacgtgtc ttgtttccta caaagttaca
    32041 tgtggaacat cacgaagcag atagtatttt tgaagtggtg aagcgcccaa accatgtgtc
    32101 taaaagttgg gaaaacccca cgaatccgca acatcagcaa gcatttgtca atattcatga
    32161 tgaagagtac ggtgtaacag tcggaaactt tggtttgaat gaatatgaag taacagaaga
    32221 cggacaaatc gcagtgacgc tacttcgcag tgtgggagaa ctaggcgatt ggggatattt
    32281 tccaacacca gaagcacagt gtctaggtga gcatcggttc aattacagca ttgaactaca
    32341 tggaccagaa gaaaaattct ctacttattt gcatgcatac gcagcacaga ttcctttttc
    32401 cactcaacaa atcaaacatc atgaaggaac attgatttca aaacaacaat atttaacgat
    32461 aaaaagtgaa acgtttgcaa ttacagcttt gaaacgcagc aagtttagtg ataaagtagt
    32521 agtaagaggt ttcaatatgt ctagtcattt agaaaaattg gaaataacta aagacaatgg
    32581 aaaaactgtc atattgaatc tgcttgaaga accaacgaag caagcggttg tcccaataat
    32641 ccaaccttat gagattcgga caatcggatt cgaggaggaa aacgaatgta tggcggaata
    32701 gaagcaggcg gaacaaaatt tgtgtgtgcg gtttcagatg gtggagaaat tttagaaaaa
    32761 ttaagtatcc ctaccacaac accagaagaa acattggatc aggtatttga attttttgat
    32821 cgttatgaat tgaagggaat cgggattggc tcattcggcc cgattggtat cgacaaaaca
    32881 aaagataatt acggatacgt tttggcaact cccaaaaaag ggtgggcaca attcgatttt
    32941 cttggcacaa tcaaggaacg ctacaatatt cctgctgctt ggacaacgga tgtcaatgct
    33001 gctgcatatg gggaattgaa gcaaggagca gcacaaggaa aaaatagttg tatttacctt
    33061 accgtgggaa caggaatcgg tgcaggtgtc gtgatcaatg aagaaatttt ttcagggatc
    33121 gcacatcctg aaatgggaca tatttgggtg aaacgccatc cagatgacca ttatgaaggt
    33181 acttgtcctt atcataaaga ttgtttagaa ggattagcag caggaccgtc tattgaggca
    33241 cgcacaggga tcaaagggca agatctgccg caagatcatc ctgtttgggc tattcaggct
    33301 tattatattg ctcaagcact gatcaactat acgttgacgt tagcacctga aaagattatt
    33361 ttaggcggtg gggtaatgaa tcaggatcat ctccttcaaa aaatccgtca gcaatttgtt
    33421 gaattgatgg gtggttatat ggaaacacct caagtaagtg aatatatcgt tcgttgggga
    33481 ctgccgaatg aaagtgggat tatcggtagt ttgctgttag cagaacacga acatcaaaag
    33541 taagcagaca aattacacaa aaagaacaga gtcttatcat tatctgctga taagactctg
    33601 ttctttttta tttctattcc cataatccag gtaaagcttt accgactatt ttttcttgag
    33661 taaatggatg gatcatgtgc agttctaatg catggagcat cagtcgttca gctgttctat
    33721 tttggtagag agggtctcca acgatcggat gtcccttatg agctaaatga actctgatct
    33781 ggtgtgtacg tccggtttcc aaaacacaat agacttgtgt ttgttcagaa agtactttgt
    33841 tgacagtgac atgtgtcaag gcgctctgac catttttatc atctacgacc ctttttctcc
    33901 gatcatggcg atctcgtcca attttattgg tgatggtcat atctttggca atttctcccc
    33961 atacgatggc ttgatatctc cgatagatca ttttttgttc caacattctt ccaagtatcg
    34021 gcaagacaaa tggatttttt gcaaataaaa cggcaccgct tgtttctttg tccaagcggt
    34081 ggacaacgta aggtcgttgc tgtttagtgt cgagataagc agctaaatga ttgagtaagg
    34141 tactgttttc tgtcggttca tttggatgag tcttcattcc tgcaggctta tccactataa
    34201 tcaaatgctc atcttcatat aaaacacgga tgtgcttcgc atctcctaat tgaacttctt
    34261 gataattata atctgtttct tcgaataaca atgtgatctg gtcattgccc tttacttcat
    34321 gatggaacaa agcaggttgt tgattgatcc aaacgttttt acgtatacgc aaaaagtgtc
    34381 gaacctttct aggaactaac cattcctttt caagcaagtc gcgaattgtt gtttcaggat
    34441 ggttcatagg taaagtgatc gtgattttca caagtgtact cctttcagtg caattgcaga
    34501 ctaattgtac catttttaaa acatatttaa gaaaaaactt acatttttct gttcattctt
    34561 atgttactct acttgcacta acgtaaaaaa acatattgaa ctacaactta agcaggaaga
    34621 aaacactctt tcactatggt aaaataagac gattgaatca aatagacagg agaagaagca
    34681 aatggatttt caaagcattt tagaaaagat aaaagctgct ttgatcagtt tttggcgttg
    34741 gatcagacca tatctcggac aattccatcg atggcgaaag cgcatctgga aaaaatatca
    34801 tgtgaacaaa ttgattttgc tgattggttt gatctgtgtc ttagtgacaa gtatctacct
    34861 ctttatttta gcaaaacaag caaatgtcga aacgttaaaa tcaggattaa gccaatcgac
    34921 agtcatttat gataagaaaa acgaagaagc tggcacactg tacgcccaaa aaggaacata
    34981 tgtagaatta aacgcgattt ctccctatat ccaagatgca gtaatctcga cagaagatag
    35041 gaatttttac gagcatcacg ggtttgatat caaagggatt gcccgtgcag cagtccgaat
    35101 ggtcttgtct ggcggaacat cagggggcgg aggaagtacg atcactcagc agcttgccaa
    35161 aaatgcgtat ctgacattag accaaacgtt tgatcgaaaa gcgaaggaat tatttttggc
    35221 aatcgagatc gaaaagaagt acagcaaaga agaaatcttg accatgtatc ttaacaatgc
    35281 ctattttggc aacggggtat ggggtgtcca agacgcttcc agaaagtatt ttggagtaga
    35341 tgcaacgaat gttacgctgt ctgaggctgc tactttggca ggaatgctga aaggtcctgg
    35401 tatttataat ccgatcgatc acccaaaaaa tgcgaatgat cggaaaaaca cagtactaag
    35461 tgtaatggaa gaaaacggaa aaatcacaaa agaacaagaa caagcagaaa gccagacaga
    35521 tatcagcgcg tatcttaatg acacatacga taattcagat tctggttatc gttatcctta
    35581 ttattttgat gcagtgatcg atgaagcagt taacgagtat ggtcttgatg aagaagatgt
    35641 catgaataaa ggctataaaa tctatacagc tttagatcag gattatcaaa agcaattaga
    35701 agctacttat caaaacagtg cattattccc agcaaatgct tcagatggtg ccatggtcca
    35761 atctgcttca gtagctttag atccgaatac aggtggtgta caagcactag taggacgaag
    35821 aggagatcat gtctttagag gattcagctt tgctacacag atgcgtcgtt caccaggatc
    35881 aacgatcaaa ccattaagcg tatatacacc agcactggaa gctggataca agccttccag
    35941 tatcttgaaa gacgaaccgc aaagttatta cgatgcgcat aattttgacg ggacatacca
    36001 aggagaagta ccgatgtatc aagctgttgc acaaagtttg aatttgcctg ctgtatggct
    36061 tttgcatgag atcggtttac aaaaaggata tgataaagca gaagaattcg gcctgccttt
    36121 agcaaaatct gataagtatt atggcttagc ccttggtgga ctggaaaaag gagtatctcc
    36181 attgatcatg gcgggtgctt acagtgcatt tgccaatgat ggtcagatgt atacgccgca
    36241 tcttatcacg aaaatcgttg attcgactgg agcggtgatc gtcgataaca caaacaaaaa
    36301 accaaaacag gtcatatcaa aggaaacagc cgatgaaatg accagcatgc ttcttggtac
    36361 attttcaaat ggaacagcgg ctgctgcgaa tcctgcaggt tatacgatag cagggaaaac
    36421 aggaaccaca gaaactaatt tcgatgccac aaaagcaaat gaccaatgga tgatcggtta
    36481 cacgccagac gtagtgattt cgacatggat gggttacgaa gaatccagca agctgcacta
    36541 tttggaaggg acaagtggaa atgttgtcgg gaaagtcttc aaatcagcgg cagaaggcat
    36601 ccttccgtat acttcaaaaa caggatttag tgtagcagat gcctatgcca caggaggaca
    36661 agtggtagct gcagatcaag ttggaaacac aggaaattct aatggagagt ctggaaattg
    36721 gcaagataac ttgaatcaat atggtgaaca agcaaaagaa gggctgaaaa acttcggcga
    36781 tatggtgaaa gaaggcgttc agggaattgg tgaagcggct aaagacctct ggcggaaata
    36841 tcaaggagag tagccccttt gaaaacgaat aaccccatgt tacaatagag agtaactact
    36901 aaaagaaaag agggcataac atgtcaaaca tctacgatag cgcgaatcaa atcgaacgtg
    36961 agatccgtga attaccagaa ttcaaagcgt tgaaagaagc gtacgagaaa gtacaagcaa
    37021 acgaagaagc acacaaactg ttcaaagact tccaagcgat gcaaatcgaa ttacaagaaa
    37081 aacaaatgag tggacaagag ttcagcgacg aagatgcaat gaaagcacaa gaaatggctg
    37141 tgaaaatcca atcagaagaa gtaatcaatg acttgatgca aaaagagcaa ggcttcagta
    37201 caatcatcaa tgacttgaac cgtatcatca tgacaccagt tcgcgacctt tacagcgaat
    37261 aaaactattt tatgaaacaa cagagggtgt gaaagagacg ttcggccttg agcgatgaca
    37321 aaattgcttc ttattgccga taggctgtct cttgaacacc atttaccaag aggtcgtgag
    37381 cctaccgttt aactctgagt tacaaggaac aatttcaaaa aacagcttct catgtttgat
    37441 gactattgtg acttgtgacc gaggagttgg caggagaaca ccgtttattc agaaatagga
    37501 gctacgacaa aagtcttgtc atagctccta ttttttgtca ttcatttttc aagcggtaga
    37561 aatagccttt ttgagctaac agtttttcgt aagtacctat ttcaacgatt tttccttcat
    37621 cgatcacaac gattttatct gcctctttga tcgttgaaag ccgatgagcc acaataaaag
    37681 tagtgggtgt ttctgtcagg taattcaggg cttgctggat ttttttctct gactggttat
    37741 ctaaagctga ggttgcttca tctagcaaaa tgatttttgg ctgacgtatc aacgctcgtg
    37801 caatggagat acattgcttt tggccaccag acagcttgtt gccatgttcg cctactaagg
    37861 tatccagacc atcaggaagc tggtcaatca gtgaagaaag ttgcgcagct tcgatgactt
    37921 catctaaacg ttcttttgat acgtttttca agccataagt gatgttctct ttgatcgaag
    37981 cggagaacaa caaagtggtt tgaggaacaa cggctaaata atttcgcaca cttcgcatat
    38041 ccagttcatc caaaggctga tcatctagaa aaatttttcc tgaagtcggc tggataaatc
    38101 caatcaataa gttaaggagt gtagattttc cagagccaga tggcccaaca aaagcaattg
    38161 tttctccagc tttcacctct agagaaaaat gatcgataat cggtttgagt gagtccggat
    38221 aaagaaaaga aacatcctca aaacgaaaat ttccatccaa atgttggatt tttttcttac
    38281 cgtgataagt ttctacatcg ccagcaatca acagttcgcc aatagaagtg atagattcca
    38341 tgccttttga aatcgtagga atcagcgtga tcattccaga aacggaattg acgatcgtcg
    38401 tgaaatacgt ctgatacatc acgacatcgc cgggttggat gcgcccttgt aaagctaata
    38461 tcccagtgaa aatcaaacaa atcagctgaa atatctgaaa actagcccag ctgactgatc
    38521 cgaaaacaga ttgcaggata tctaagtgga atcctttttc atagatttga gtaaaatgct
    38581 tatttaagcg agttgtttcg tgtttttcta acccatgagc tcgagcaatc ggtgctaatt
    38641 cgatcacttc gatcactttt gcagaagtct cttccatttc ttgcctgaag tctgagttgg
    38701 actgtctgat ccttttacgg aaaaagagag taacgaatgc ggcgagagga acagtcaata
    38761 agaaaaacat gaaaacaaca ggacttttat aaagcgtgat acctaaagct acgaataaat
    38821 tgacagcgat atttaatccg ctgacaaata cttgagagga caacgtttgg atagcttcta
    38881 catcacggat gatctttgac tggattcgtc cagattgcaa ttctttttga taaggaatag
    38941 atagttcttg gattttcttg ataagggtcg ctcttagtcc agcttctact tgacggatcg
    39001 aagtgctatg gtattttaca tgaaaataat tcgtcaatat attttgacca atcagacaag
    39061 ccatcaaaaa agcgttgaaa taaatggcct gcaactgaga agaatctttc gatgaagctg
    39121 cattgattac attggcaata gcgatcggca ttaaccaaga tggaagatgt ttgataataa
    39181 aaaagagtcc tgacatggca aaacgccaat aatttccctg ataaagatgc agcaatgtct
    39241 gaaaagcatt tgcatgatat ttttgataac ttttctgtat tcgttcctcc ataatcttct
    39301 ccttaacaat cttggtaaat aaactgcaag tgttctattg cttcaggaac ataaatctca
    39361 ggagtagctt caagagtggc gtacattaga ggacgctcat atttgatata atgcagtacc
    39421 gggcggaaat ctaaacagcc cttgccaaca ggtaccatgc ggatctttcc attttccatg
    39481 atatagtctt tgagatgaag cgctgtaata taaccatgta aattgtccaa ggcacctcgg
    39541 atcacttttt cctgatcagc atgattttct ttatgcatca aattcgcaca atctaaaatg
    39601 atttttaaat ttggtgaagc tacttcatcg attaaacgtt tagctaactg atagttatag
    39661 attggatgat tgatccctgc ttcaacagca acagttacgc caaaattttc tgcatgagcg
    39721 actaattcaa tgatagaatt tctggcgatc tgataagctt cttcagtaaa attttctttt
    39781 gtgtaaccga cagagacact accggtttcc gtagcaatca tcttggcttg ataggcagag
    39841 cacagagcaa tttgctgttt gaaagttgcc aatgcttgtg cacgaacttc ttgatttttg
    39901 ctggataggt tgatatagca tcctaaaatg gaaagttcga tattgttttt ttggaagtat
    39961 tcaccaaaat aggcagcact cccaagtgtc atgtcagtta atttaagttg ttcaggaaat
    40021 gctttttgtg gtgcaaattg gatatgggac aagtgaagct gtgatacttt ttgaacgatt
    40081 tcttcacgat ttcgaacatc taaatcgtgg gcacggatgc ctaaatttaa tggaaacatc
    40141 tcaaactcct ttttgtaaat aattagcaag aggcagacgg gattctttta tcaattcaac
    40201 aattattcgg gcaactttat cagcgccttc tggactaaag tgggtattgt cttttagtcc
    40261 ttctgggtaa tttttacaag tatcaggcat aagatgcagg tagaattttt ttgtctcttc
    40321 ttttgagaag gtctggaaca attcttgtga tcttgagaac acatctaaaa gaggaatatg
    40381 gtgtttctca gcaaatgcac gcatcgcttt tggatagtct ccaagtatat ctggattcaa
    40441 tgttccgttt tctaagtaat ctcgtcgcgt cacagatgtc agtaagacca tttggacgtt
    40501 cgcttttttt gcgcattcaa aaaatatctg cagatttttt aaatagctac cgaagggaag
    40561 cgtacctctt tcttcttgga ttttctggtc gttatgtcca aattgtacaa ataagaaatc
    40621 attcggctga aactgtgaag ctagacgggc taaccttcct tcctggataa atgatttggt
    40681 gctgcgtcca ttctttgctt ggttattgat ctggatctcg ggtgaaaaat aagcagagat
    40741 tttttctccc caacctgtct cgggacgctt gttttcatct ttttctgccg cagtagaatc
    40801 accggcaata tggatagtag gcatgttatc tcttctttca taaaggttta taggcaatga
    40861 agcagaacag caagagaaga agacggagca a
//
LOCUS       DAJSBL010000021        39152 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.021, whole genome shotgun sequence.
ACCESSION   DAJSBL010000021 DAJSBL010000000
VERSION     DAJSBL010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 39152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39152
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.021"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..290)
                     /locus_tag="ONT85_001302"
     CDS             complement(<1..290)
                     /locus_tag="ONT85_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HCR2378288.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTET"
     gene            complement(283..624)
                     /locus_tag="ONT85_001303"
     CDS             complement(283..624)
                     /locus_tag="ONT85_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2378289.1"
                     /translation="MNKELSTLDQYLTDSEWGKSNIKETNNRKIRRNLLTNEELSCDQ
                     DDLGNFVSIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYNRDNLIDISYYGSGKK
                     YIAEMEAEFDE"
     gene            complement(798..1523)
                     /locus_tag="ONT85_001304"
     CDS             complement(798..1523)
                     /locus_tag="ONT85_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HCR2378290.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFKKKHKHVLEAIESIKRSVENS
                     ANVEDGSNFGQMFVEGNEPDSYGRSRRVYFMNRDGFSLLAMGFTGSKAINFKLKFIEA
                     FNEMEDVIRKNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNM
                     KVMEVLGGKESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLP
                     ETAIRMEIDQLNRQQRLFGDENE"
     gene            1576..2052
                     /locus_tag="ONT85_001305"
     CDS             1576..2052
                     /locus_tag="ONT85_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2378291.1"
                     /translation="MRKKLNYILTATLTIYIILFITLSFLSSIKKIPFITLNKNSILK
                     EFNNTDYIAFLKLFSVCVLISCVIIFIVAKTCWTMLNKNQRDVENIAIFLIKITSSIS
                     IIIPMTVTFSKEQFNVAATYLSFIALFSFVLPKRNKSNFDNKELDEVRNSSPKEQK"
     gene            complement(2058..2240)
                     /locus_tag="ONT85_001306"
     CDS             complement(2058..2240)
                     /locus_tag="ONT85_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2378292.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEHRAKKASREGLTKI"
     gene            complement(2240..2539)
                     /locus_tag="ONT85_001307"
     CDS             complement(2240..2539)
                     /locus_tag="ONT85_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2378293.1"
                     /translation="MKKPTLSELIEAAEKAVKPDDWYRQSLILEKFHGMSKTTLVEYC
                     KEMETIPEFAEGILRPGHSTTFIHYHTFIWFLKWKDANKYRVKSLSPSDVLKEAS"
     gene            complement(2644..2847)
                     /locus_tag="ONT85_001308"
     CDS             complement(2644..2847)
                     /locus_tag="ONT85_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR2378294.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            complement(2862..3608)
                     /locus_tag="ONT85_001309"
     CDS             complement(2862..3608)
                     /locus_tag="ONT85_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HCR2378295.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVADVLGYSNPQKAIR
                     DHVDLEDKTQNDSFTVNGTAVVLINESGLYSLILKSKLPSAKKFKRWVTSEVLPTIRK
                     TGSYSNVPQSFAQALRLAADLEEKNQLLEQQIAEYEPKISYLDTILSSTDTVATSQIA
                     ADYGMSAIALNKLLNELGVQHKVSGQWILYRKHMNQGYTKSHTSEIPKADGGTKVVMN
                     TKWTQKGRVFIYNLLIAEGYYPQMDLEEIG"
     gene            3686..3994
                     /locus_tag="ONT85_001310"
     CDS             3686..3994
                     /locus_tag="ONT85_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2378296.1"
                     /translation="MKPEFVITMLDGEKIKIFADTLVVGYDDASNIEEKDGMFYSSQI
                     YVNTLQGDIGVSTLATSRPLVGLIGFLSKATFFSLGDDIDSDETVIYKTSAVKSIGFK
                     "
     gene            complement(3965..4222)
                     /locus_tag="ONT85_001311"
     CDS             complement(3965..4222)
                     /locus_tag="ONT85_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase"
                     /protein_id="HCR2378297.1"
                     /translation="MNKEMSQTIKVQKMIDDLTHGIDSQADKIIKELQGQKVKDAKML
                     LKTINFEMNPTKRKLADVLEEKLASAINEQELLFETDTFNS"
     gene            complement(4520..4660)
                     /locus_tag="ONT85_001312"
     CDS             complement(4520..4660)
                     /locus_tag="ONT85_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2378298.1"
                     /translation="MEKTVSAKIEDLKLDILKQAKVAMEHAVDKEDSAMVAAIAEILA
                     HV"
     gene            4960..5280
                     /locus_tag="ONT85_001313"
     CDS             4960..5280
                     /locus_tag="ONT85_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR2378299.1"
                     /translation="MNTYEIIKELAKEKGLSIRQLEMNFGYSNGYLGSWKRQTPNSIE
                     LARLADYFGVSVDYLLGREERETPKHVDLSENDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            5298..5729
                     /locus_tag="ONT85_001314"
     CDS             5298..5729
                     /locus_tag="ONT85_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HCR2378300.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFTASHFEYSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
     gene            5856..6440
                     /locus_tag="ONT85_001315"
     CDS             5856..6440
                     /locus_tag="ONT85_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HCR2378301.1"
                     /translation="MAKKVMGQDGKMYKVSKPFYKKVWFWVLAVILIIIIGSALNGGS
                     DSNKASDNGGEKVTKSSTSASSSKEEKSDTFYKIGDTVKVGDAEYTLNSVELTDERNQ
                     FEENQPAQVVKITYTVKNDGDSDIPVGTDVEVYGPDDKKSETYANENTMGSVAPGKQM
                     DVTAHFTLNQKGEIEIHFSPLVSFEKAAIFKATV"
     gene            6542..7690
                     /locus_tag="ONT85_001316"
     CDS             6542..7690
                     /locus_tag="ONT85_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR2378302.1"
                     /translation="MLKVWVEQTKDGQFKFIERYVDPYTEKTRKKSTTLTSNSPQAWK
                     KAQKILDKKIKEALEDYNKSDITFGELYKEWYEYYKQHVKRTSYLKVPMMMKHVSKHI
                     SDDTIVRNIDETLINKIIEDMYTFGDLSLNYTKQTKTTLSVMLNYAIDRKYIQRNPAL
                     AVKIHPKKVEEEKRRLSMNKKYLEKEEIDQILKQLYSNPRRKLHGIIAEFLYLTGLRY
                     GELLALQMKDYEDGKISINGTLDYTSVKMDNAIKTTPKNTYSQREVQLPNRAKELIES
                     VIADNILAGRPTDPDQYIFISTSGTPLALHSFNAILHKVEEELELEKSLSSHIFRHSH
                     VSLLSELGVPLKAIMERVGHSDANTTLSIYNHVTKRAKQQVIDKLNSL"
     gene            complement(7779..9404)
                     /gene="groL"
                     /locus_tag="ONT85_001317"
     CDS             complement(7779..9404)
                     /gene="groL"
                     /locus_tag="ONT85_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HCR2378303.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDATIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
     gene            complement(9455..9739)
                     /gene="groES"
                     /locus_tag="ONT85_001318"
     CDS             complement(9455..9739)
                     /gene="groES"
                     /locus_tag="ONT85_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HCR2378304.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            9964..10626
                     /locus_tag="ONT85_001319"
     CDS             9964..10626
                     /locus_tag="ONT85_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HCR2378305.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            complement(10702..11994)
                     /locus_tag="ONT85_001320"
     CDS             complement(10702..11994)
                     /locus_tag="ONT85_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCR2378306.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQHLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            12164..12793
                     /locus_tag="ONT85_001321"
     CDS             12164..12793
                     /locus_tag="ONT85_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HCR2378307.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAASDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            complement(12896..13705)
                     /locus_tag="ONT85_001322"
     CDS             complement(12896..13705)
                     /locus_tag="ONT85_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HCR2378308.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(13760..14629)
                     /locus_tag="ONT85_001323"
     CDS             complement(13760..14629)
                     /locus_tag="ONT85_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HCR2378309.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            complement(14630..15946)
                     /locus_tag="ONT85_001324"
     CDS             complement(14630..15946)
                     /locus_tag="ONT85_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2378310.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            complement(15943..17778)
                     /gene="walK"
                     /locus_tag="ONT85_001325"
     CDS             complement(15943..17778)
                     /gene="walK"
                     /locus_tag="ONT85_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HCR2378311.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVILSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            complement(17783..18487)
                     /locus_tag="ONT85_001326"
     CDS             complement(17783..18487)
                     /locus_tag="ONT85_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR2378312.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            complement(18677..19537)
                     /locus_tag="ONT85_001327"
     CDS             complement(18677..19537)
                     /locus_tag="ONT85_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HCR2378313.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITPEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            complement(19524..20093)
                     /locus_tag="ONT85_001328"
     CDS             complement(19524..20093)
                     /locus_tag="ONT85_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HCR2378314.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            complement(20667..21836)
                     /locus_tag="ONT85_001329"
     CDS             complement(20667..21836)
                     /locus_tag="ONT85_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR2378315.1"
                     /translation="MKLRANIIEKRGTLQVVVSYKDSNGKIRQKWKDTKLKKKGNKKR
                     AEKIRDEFLLELESQLAMECECANPNLLFYDYLLNYLEVSKKQVAFNTYVGYKHYVHN
                     RIYKFFYPKKIKLNRLKPYHLQEFYQYMLNDNCTANTVIHYHAFIRKALQEAVITELI
                     STNVADKVRKPKKKQFVSQVYNQQEILKLLDILSEEKLYLVVLLTAFYGLRRSEVLGL
                     KWSAIDFLNKKIMINHVIIENPENMSQLIKKDQTKNSSSYRTLPLVETIERALVNQAK
                     WQQDNRILLGDDYILEDNEYVFTMEDGRLMKPQYVTHRLSKLIKKNGLKKIRFHDLRH
                     SNASIMLDSGQNMKSIQEWLGHASYSTTANLYTHLTAGVKDRAAEALENAFGFKE"
     gene            complement(21880..22110)
                     /locus_tag="ONT85_001330"
     CDS             complement(21880..22110)
                     /locus_tag="ONT85_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR2378316.1"
                     /translation="MDFQDYEKIFESYPSILKITDLTKMLNIGKNKAYNLLKDRSIEN
                     IKIGARYKIPKKAVIEYILKIQDEGKNHAIIK"
     gene            22186..22258
                     /locus_tag="ONT85_001331"
     tRNA            22186..22258
                     /locus_tag="ONT85_001331"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22219..22221,aa:Lys,seq:ctt)
     gene            22420..22641
                     /locus_tag="ONT85_001332"
     CDS             22420..22641
                     /locus_tag="ONT85_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2378317.1"
                     /translation="MRNEIANITIIRETLQNHTANEISKHTGLNLSTIKKLKSGERSI
                     EKLNLHDAICLTEFGLKNNRKNVEINIWK"
     gene            complement(22965..23210)
                     /locus_tag="ONT85_001333"
     CDS             complement(22965..23210)
                     /locus_tag="ONT85_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR2378318.1"
                     /translation="MVDNPKKRQYPLVPLSIIQSALEEDPISIKMILKHYHSYIAKLC
                     LTNGFNEAGQFITYVDEYMLRQLEIKLIEAILKFKIN"
     gene            complement(23204..23605)
                     /locus_tag="ONT85_001334"
     CDS             complement(23204..23605)
                     /locus_tag="ONT85_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HCR2378319.1"
                     /translation="MQQNYNEKSIQHQFDAYCKKVLRNETKSIRKRNINITDNEEPLT
                     NSNEGKYSQTHFDEQDVYFLFGMEILISDQKLSAAIDHLSEIRRKVILLYYFAGFNDT
                     EIGKILNMSTSGIWYQRKKAVEQLKMGYDLW"
     gene            complement(23777..25585)
                     /gene="uidA"
                     /locus_tag="ONT85_001335"
     CDS             complement(23777..25585)
                     /gene="uidA"
                     /locus_tag="ONT85_001335"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HCR2378320.1"
                     /translation="MLYPIMTETRQVIDLSGLWRFKLDTNDDLQEELALEPMVEKESY
                     PISVPSSYNDLFETENIRNHVGWVWYEKEVILPKKLLEERLVLRFGSATHEAKVFLNG
                     KLINQHKGGFTPFESEINQFIKDGSNRITVAVNNVIDDSTLPVGILKETIQEDGDIKK
                     ENLVNFDFFNYAGINRPVKLYTTPKDYIKDVTINTKIIDSDANVEFNIDTEWTGEILI
                     AIYDEEAQLIAEAKGKEGVIQLKNPTLWEPLNAYLYSMKVSLINENKVIDTYTEEFGI
                     RSIQVSNGKFLINNKPFYFKGFGKHEDTYINGRGLNEAVNIKDFNLMKWIGANSFRTS
                     HYPYSEEIMRLADREGIVVIDEVPAVGLHLNFMATLLDDNVEKHDTWKEIRTENAHKM
                     AIKELIERDKNHPSVVMWSIANEPDSDSEGAKEYFEPLVELTKELDPQKRPVTIVTYL
                     KSTPDICKVGDIVDVLCLNRYYGWYVSGGDLKHAKYLLKKELDGWMERCPDTPIMFTE
                     YGADTVAGLHDTVPTMFTEEYQVGYYEANHEVIDKCDNFVGEQVWNFADFETSQGILR
                     VQGNKKGIFTRDRKPKMIAHYLKKRWSSIPNFSYKK"
     gene            complement(25613..27379)
                     /locus_tag="ONT85_001336"
     CDS             complement(25613..27379)
                     /locus_tag="ONT85_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003056564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-hexosaminidase"
                     /protein_id="HCR2378321.1"
                     /translation="MTIKVDLRKKPYHLDEDAISWIEETIDAMTLEEKIGQLFVNMGS
                     SRDKNYLSSMVNQYHIGAVRYNPGTADEVYDQNYILQTESKIPLLIAANTEAGGNGAC
                     TDGTEVGVEVKIGATNDAKYAYELGRVSGIEASAIGCNWSFAPIVDINRNWRNPIISS
                     RSFGQSPDKVLELSLAYMKGIQESGILPAAKHFPGDGIDERDQHLSFSINSLSTEEWD
                     NSFGKVYSGLIEAGLPSIMAGHIHMPAYSKHFNLDLKDEDLLPATLSKELITDLLRGK
                     LGFNGLVVTDASHMLGMTGAMKRSELLPTSIAAGCDLFLFFNDPDEDFNYMMEGYYKG
                     IISEERLRDALRRILGLKAKLGLHKKEKNKILLPKQEAMEKIGLIENKSIFKEVADQA
                     ITLVKDKQDIWPIVPEKYKRILLVDVHGVKGGFGEVVAGNRPQAIDLLKQILSAKGFE
                     VEKYISPEEELKGLSQEEKIKNIAGIYAAKRPIKDLTEKYDLIINIANVNPGTVQRIV
                     WPASKGTPDIPFYIHEIPTIFVSVQCPFHLADVPQVKTYINTYDGKQETLEILVDKLM
                     GLSEFKGISPIDAYCGLKDTFI"
     gene            complement(27392..28186)
                     /locus_tag="ONT85_001337"
     CDS             complement(27392..28186)
                     /locus_tag="ONT85_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HCR2378322.1"
                     /translation="MNSWIFCIDSDGCVMDTMTYKHNLFFGPIAAEIFDVHHREKFLQ
                     DWNNINLYSETRGINRFLGLVKTLEKNDVPSINRLKHWVSVTKELSNPSLKREIEYSQ
                     SEDLLKALKWSEEVNRQISQAKGLDKPFLSARDTIKALKKYGKIIIVSSANKEAIQEE
                     WERHELLSLVDELCCQDKGKKEDIIRSVIENGCDLDKILMIGDSPGDLEAANKNKVFF
                     YPILVNKEKESWENLRTDILFKCLSGEYIDIEQKYIDTFWKNLTNK"
     gene            complement(28197..29573)
                     /locus_tag="ONT85_001338"
     CDS             complement(28197..29573)
                     /locus_tag="ONT85_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR2378323.1"
                     /translation="MERKFGLRDKIGYMFGDFGNDFTFIFASSFLMIFYTKVLGISGA
                     TVGTLFLVARFIDAVTDITMGRIVDGSKPSKTGKFKPWILRMSGPVALASFLMYQTSM
                     ASASMSIKVIYMYVTYILWGSIFYTSINIPYGSMASAITSVAEERTALSTFRSIGATL
                     AGMFIGVLTPIFIYTRDSSGAQIVRGGSTFTIVAGVFSVCAVICYVICYKLTVERVKS
                     DESQLKVSLLKSLKGLAKNRALLSIIAAAIFLLLAQLLTMSMNNYVFPDYYNNAQGIV
                     LMNFINPILMLVLVSPVSLIFSKKYGKKELASVGMFFSAIVYLLLFLIKPSNMYVFLV
                     LTSVGYMGLGIFNTVIWANITDVIDDQEVKSGQREDGTIYAVYSFARKIGQALAGGVG
                     GWTLSIIGYDSLAKVQSGEVLQKLYNSSTLIPAVCFFVVGLILFFVYPLSKEKVLENY
                     NLLNQKHN"
     gene            complement(29702..30349)
                     /locus_tag="ONT85_001339"
     CDS             complement(29702..30349)
                     /locus_tag="ONT85_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HCR2378324.1"
                     /translation="MKKVEILSKLEAAGVIAVVRGKNKDEAIKAINAITAGGIKGIEL
                     TFTVPNAEEIIKEIAKKYADQKDVIIGAGTVLDAFTARIAIMAGAEYIVSPSFDRQTA
                     EICNLYQIPYLPGCMTITEIKEALTAGVEIIKLFPGSAFGPSVVKTYQAPLPYVNIMP
                     TGGVSLDNLEEWFEAGVVAVGVGGNLLAPVEKGDFEEVTRIASKYIDKFYEIKNY"
     gene            complement(30433..31860)
                     /gene="uxaC"
                     /locus_tag="ONT85_001340"
     CDS             complement(30433..31860)
                     /gene="uxaC"
                     /locus_tag="ONT85_001340"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HCR2378325.1"
                     /translation="MFLDKNFLLENEIAKRLFHKFAEHQPIIDYHCHLDPACLLENKQ
                     YKNLTQVWLNDKGAGDHYKWRLMRANGIEEELISGAGNDYEKFLAFVKTVEKAPGNPI
                     FEWSHLELQRYFDIDLTISEKNAPEIWRRANEKIQTDSYRPKELLKMMDVKAICTTDD
                     PTDDLRYHTELSKQDNSFKVLPTFRPDKVWSIKSSGFSNYILKLSQAAGIPIKNFNGL
                     KQALSQRVDYFHQIGCRLADHGLNTYFYEIADEKILDNLLIKALAGKTDFHLSEIAQF
                     VTGIQLHLMSEYTRYGWTMQMHLNALRNNSSILKEQIGVDVGGDSMGDQASLTQNVVA
                     LFDTAEKSGCLPKTILYSLNPTDWLPLATAMQSFQGGMIQKIQLGCAWWFNDTAEGMR
                     QQLTTMAQQSLLSNFTGMLTDSRSFLSYPRHEYFRRVLCQLLSEWVKAGRIPDDEMYL
                     SQIVENICYRNAYNYFNFFEIESRK"
     gene            complement(31934..33013)
                     /gene="uxuA"
                     /locus_tag="ONT85_001341"
     CDS             complement(31934..33013)
                     /gene="uxuA"
                     /locus_tag="ONT85_001341"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HCR2378326.1"
                     /translation="MEMTFRWYGANEEKISLQEIRQIPAMKGIVGTLMDIPAGEVWPR
                     ERIKALKDEVEANGLTLKVIESVNVHDDIKIGLPTRDQYIENYKQTIRNLAEFGIEVI
                     CYNFMPVFDWVKSDLDYHLDDGSSTLAYISKNIPTDPEEIIRKVENSTNNFELPGWEP
                     HRLSQIKQLLEAYKEVDEAKLRENFAYFLKAIIPTCEEVGIKMAVHPDDPPYSIFGLP
                     RIVKNRDDLEWLCNVVDSPSNGITLCTGSIAERPDNDVYEILSEFCQRDRIPFAHVRN
                     IKYLSDNGLDFYEAPHKSEYGSLDMYKILKTMYDNGFNGYIRPDHGRMIWGETGRPGY
                     GLYDRALGAVYLNGIWEALEKGASK"
     gene            complement(33016..34647)
                     /locus_tag="ONT85_001342"
     CDS             complement(33016..34647)
                     /locus_tag="ONT85_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HCR2378327.1"
                     /translation="MVLIKDWQDSIEGLNKMQVQLPYFNVEAVKQEGQKHPVWLHFGG
                     GNLYRGFHAEIAQCLADQNELESGVVVCETFDEEVVEKAYHDYNNDILEIVMGEDGEL
                     KKRLLAVTADSLYCNPKNITDYQRVVKYFEDPTLQFTTFTITEKGYSLKDSKGQFFSV
                     VEADIKSGPTNPQHTMSIVTSLLYSRFNAGELPIAMVSTDNFSQNGQRFQDSIFMIAE
                     AWQKKGYVSEKFIAYLKDKEKVTFPWSMIDRITPNPSESVKMDLLKAGFEDLDIIHTA
                     KNTNIAAFTNTEKVHYLVIEDSFPNGRPKLEAAGVIMTDRETVDKADIMKVTTCLNPL
                     HTALAITGCLLGYKSIQAEMQDDDLVGLIKGIGYVEGLPVVESPGVIDPKQFIDEVIN
                     KRLPNPYIPDTPQRIATDTSQKVAIRYGETIKKYVEKDGNAENLTFIPIAIAAWLRYL
                     LAVDDQGKNFVPSPDPLLVELQEKFSVLKLGEQDPAKIHCAVQEILENKEIFGLDLYQ
                     VEIGKKIEKIFCEMLVGPQAVRKTINKYITEVGGK"
     gene            34964..35635
                     /locus_tag="ONT85_001343"
     CDS             34964..35635
                     /locus_tag="ONT85_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR2378328.1"
                     /translation="MTIKTSLQNQAYQSIRQQIIYADLEPGKKVSEKKLEETLSIGRT
                     PIREALIQLRHQGLVDTIPQSGNYISLIDLDLAKNARFVREHLERQIMLECCAKMNNQ
                     KKQVLETIIAEQEKAVSQKNARSFFHNDNLFHKACFEIAEREEVWQWLEDNNTHLERF
                     RWLRVKTEGLKWEKIMEQHHQLFKALTMQDPEEANFLTSLHLHLMLDEQNVVLTTHPK
                     YFKNI"
     gene            complement(36471..37427)
                     /locus_tag="ONT85_001344"
     CDS             complement(36471..37427)
                     /locus_tag="ONT85_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2378329.1"
                     /translation="MLILANQASTFVQTQQLRGRGLRLGAQNKVTNIWHLAIGLPGVP
                     LSEQPDLKRMLRRLGNISGLTFESSPKIESGGDRFALPLEYTTTTMETYTQSMFYFSC
                     ERSRLKEQWSTALADGWHLSMPIVVRKVDQVKQKQETSLPIVVKNIEPLSFWSAIFKG
                     NLLEYFQIRRRQKLWEKECARKKSLAICVQTTLQRLGEVSDRKVIVAADQQEFRCYLE
                     NAGYREGQLFTEAMRELLAPLENPRYLIEGKKIVLTVPERFGRNKQIANLFLNELNQS
                     SLPKCQLVYTRNPEGRQKLVTYRMRQVFQTRDEVIIQKNIWT"
     gene            complement(37536..37781)
                     /locus_tag="ONT85_001345"
                     /pseudo
     CDS             complement(37536..37781)
                     /locus_tag="ONT85_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347587.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(37926..38348)
                     /locus_tag="ONT85_001346"
     CDS             complement(37926..38348)
                     /locus_tag="ONT85_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2378330.1"
                     /translation="MVKEEVLCPHQDFLYLIPANQETTQHYFTFLEKQNELVEALMES
                     QETVDYLLSLEFVQHPLEHSKEIYENFELFTSALIYLNHHNYELIPEHWQLLGSSKQQ
                     GKKASVPSLGKKELITIYQQMFEDQPELPIFYQLKKAG"
     gene            complement(38528..38899)
                     /locus_tag="ONT85_001347"
     CDS             complement(38528..38899)
                     /locus_tag="ONT85_001347"
                     /inference="COORDINATES: protein motif:HMM:NF016724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HCR2378331.1"
                     /translation="MWLSDEKIQWRSYQKRILTNFATYLNNNSVHLVAPPGAGKTFLG
                     IEMIRRLDCKTLILVPSLILKKQWLTTIEEYFLVNPADSCYLSGAIEHPSHITVETYQ
                     TIYNRLKEEPTYGDQLELLVF"
BASE COUNT        11878 a         7805 c         6020 g        13449 t
ORIGIN      
        1 tctgtttccg tatgtccttt aaccgttact gagacagata cataagtccc tgtttcatcc
       61 ataagaaaag gtttatattc ctcaacaaga aagtcttgat gagttccaga aacaaccctg
      121 tagtgtttat actcattaat agttaccgtt gcctgtggat cattcttttt cataatctcc
      181 cacgcgtgag cccaagataa ataatcaaaa tttccttttt tcttgagaat tttatttaac
      241 ttacgactaa aaagtttttc aaagttcgtt gtccctttga tttcactcat caaattctgc
      301 ctccatttca gcaatgtatt tcttacctga tccgtaataa gagatatcaa tcaagttatc
      361 tctgttgtac tcttctagcg catcaatcaa gccatcttcg atgacataga tgtattcagg
      421 tttatttgaa tgttttgata gatggataag gtaaacatga tcccaaatac tcacaaaatt
      481 tcccaagtca tcttgatcac atgatagttc ttcgttcgtc aaaagatttc gtctgatttt
      541 tcgattattt gtttccttga tattcgactt gccccattca gaatcagtca aatattgatc
      601 taaagtggaa agttctttat tcatatgcta aaattctcct tagatatgtt ttgtgactct
      661 atgcttgccg gctgagtcac ttttttattt gttgccatgc tttttgcttt tcgatatgtt
      721 gttggcttag gatgtttggt ttattgtgtc tccaccagcg attagcaatt accgtcccta
      781 ttcttagcgc ttcagctcta ttcattttca tcaccgaaaa gtctttgttg tctgttcagt
      841 tgatcgattt ccatgcggat cgcagtttct ggtaaccaca tttcaataaa tgaaacagca
      901 tcatcgaatc tcttacgagg taactcgcca tatcttggga ttgaaaaggt acgtttaaat
      961 tcagaccaaa attttgagaa tacttttttg ctgatttctt cataagctcg gctttctttt
     1021 ccccctagaa cttccataac tttcatattt cctttttgct taatttcaaa ctcttgttgt
     1081 ccgctaattc gcatagtatc tttaagcatg gaaacatctt ttttaacatc tttcatttct
     1141 tctaattgat agatcatcat gtcttcaatt gtttgaggaa cagtattctt ccgaataaca
     1201 tcttccattt cgttgaaagc ttcaataaat tttagtttga aatttattgc tttacttcca
     1261 gtgaatccca tagctagcaa ggaaaatccg tctctattca tgaaataaac tctccgactt
     1321 cttccgtatg agtctggctc gttcccttcc acaaacatct gtccaaaatt ggacccatct
     1381 tcaacattgg ccgaattttc gaccgatctt tttattgatt caattgcttc tagtacatgc
     1441 ttatgtttct ttttgaaact ttctgccact tgtaagctcg tagttacagc ttctttattt
     1501 ttcaaaatta ctaattcttg cattatttct tctctccttt ttggtataat tttcgttaga
     1561 aagtgaggtg attctatgag gaaaaagctc aattacattc ttactgcgac tttaaccata
     1621 tatataattc tgtttattac cttatctttc ctgtcttcaa ttaaaaaaat tccttttatt
     1681 acattgaata aaaatagtat tttgaaggag ttcaataaca ctgactacat cgcatttcta
     1741 aaactttttt cagtctgtgt gctaatatca tgtgtaatta tttttattgt tgctaaaaca
     1801 tgttggacaa tgttaaataa aaaccaacgc gatgttgaaa atattgctat atttttaatt
     1861 aagattactt cctcaatatc aattatcatt ccgatgactg taactttttc aaaagaacag
     1921 tttaatgtag cggctactta tctttcattt atagcgctat tttcttttgt tcttcctaaa
     1981 agaaataaga gcaactttga caacaaggag ctggatgaag tacgcaatag cagcccaaag
     2041 gaacaaaaat aaaaaaatca tattttagtc agtccctccc gactggcttt tttcgctctg
     2101 tgctcagctt catcaagccc cataaaaatc caaaccatgt aaacgattgt ccctatcaac
     2161 gcttgtctgc ttccccagag gcctaaagcg tagatgatta gcggtgcgct gaatactagt
     2221 gctctgttga atttgcccat taacttgcct ccttcaaaac atctgaaggc gataaacttt
     2281 ttacacgata tttgtttgcg tctttccact ttaaaaacca aataaaagta tggtaatgaa
     2341 taaatgtggt tgagtgacct ggacgaagta ttccttctgc aaattcagga attgtttcca
     2401 tttccttgca gtattcaact aaagtagttt ttgacatgcc gtggaacttc tccaagatta
     2461 aactttgtcg gtaccagtcg tctggtttta ctgccttctc agcagcttct atcagctctg
     2521 aaagtgttgg ttttttcact tattatccct cctaaaaatt atcgttccat actcgatgtt
     2581 taacatttct tgaagctctg attttgtaag aatgatttta tcttccaacg actttacctc
     2641 cctctacaat tcgtacatag tgataatcga atctataatt ctgtttgctt ctgcagaagt
     2701 ctttttaccg tttaaaatta aagataagta gcttttgcta attccaaatc tttcagcaag
     2761 catggtgtaa gttaagaact ttgaactttc gacatattct ttgatttttt ctctatctcg
     2821 ttgagtgatt tctgcaatgt cagtcatact aaaactcctt tctaaccaat ttcctctaaa
     2881 tccatttgag ggtaatagcc ctctgcgatt aataagttgt aaataaacac tcgccctttc
     2941 tgtgtccatt tggtattcat tacaacttta gtgccgccat cggctttcgg tatctcactt
     3001 gtgtgcgatt ttgtgtatcc ttggttcata tgttttcggt aaagtatcca ttgtccgcta
     3061 actttatgct gaacacctaa ctcgtttagc aatttgttta gagcaattgc cgacattccg
     3121 taatcagctg caatttgaga agtagctacc gtatctgtcg atgaaagaat cgtatctaag
     3181 tagctaatct ttggttcgta ctcggcaatt tgttgttcga gtaattggtt cttttcttct
     3241 aaatccgctg ctaaacgcaa tgcttgtgca aaactttgag gtacgtttga atagctacct
     3301 gtttttctaa ttgttggaag gacttcactc gttacccaac gtttaaattt tttggcagag
     3361 ggaagttttg attttaagat taaactgtaa aggcctgact cgttaatcaa aacaactgct
     3421 gttccattga cggtgaacga atcgttctgc gtcttatcct ccaagtccac atgatctcga
     3481 atagcttttt gcggattcga atatcctaga acgtctgcta catcttttcc tacaaaatat
     3541 ggttcatcat ttactaaaat agttcgaact tcgttttgct cgaaattaaa aatttgtggt
     3601 gtattcatat ttctcattcc tttctttggt ataatttcct tatcagtgtg acaggctgaa
     3661 ataattgtta aggaggtgaa aatttatgaa accagaattt gtaattacta tgcttgatgg
     3721 agaaaaaatc aaaatatttg ccgatacttt ggtcgttgga tacgatgatg cgtcaaatat
     3781 cgaagaaaaa gatggaatgt tctattcttc tcaaatttac gtgaatacct tgcaaggtga
     3841 tattggggta tctactcttg caacatcaag accgcttgta ggtcttatcg gctttttgtc
     3901 aaaagcaaca ttcttttctc tcggcgatga tatagattcg gatgaaactg tcatctataa
     3961 aacatcagct gttaaaagta tcggtttcaa atagtaattc ttgctcgttg atagcggaag
     4021 ccaacttttc ttccaacaca tcggcgagct ttctttttgt tggattcatt tcaaagttga
     4081 tagtcttgag aagcattttt gcatccttaa ctttttgtcc ttgcaattcc ttgatgattt
     4141 tatcggcttg gctatcaatt ccatgtgtta gatcatctat cattttttgt accttgattg
     4201 tttggctcat ttccttgttc attgtttagc ctcctttaat aaatgatttt ttgtattttt
     4261 taatttccat aaattcctca aattgctctt tggtattatt accacgacca tacatattgt
     4321 gaaattcttt atggcaactg acacaaagtg taacgccgtt tgaaatcgct gcggatttag
     4381 ttgttgcatg gctaaatcac gaatcacaag tatctactaa aggcgaatca atttcgccaa
     4441 aagaaattgt tcaagcttat ttagatattc attatgctgt tatctatggt cagcttccag
     4501 aagaccgtaa aaacgatgac taaacgtgag ctaaaatctc tgctatggct gcaaccatgg
     4561 cagagtcttc tttatctacc gcgtgttcca tcgccacttt tgcttgtttc agtatgtcta
     4621 atttcaaatc ttcgattttt gccgagactg ttttttccat attcttctct cctttctttt
     4681 atattcgtaa acaaatttaa caacttttta aaaaactgcg ttgactttat tagattaata
     4741 atctatacta aatgcatagt taaataagac atataaactt tgatttaaaa agctttcttg
     4801 gcggttggca tttattaatc aatagtattt tttgttgtct ttttagttgt taaacttgtt
     4861 tacaaaacat agtatagatt aataatgtat ataagtcaac tatttttaca ttatttatct
     4921 aaactttttt tgtaagcatt cagaaaggtt gatttatcaa tgaatactta cgaaataata
     4981 aaggagttag caaaagaaaa aggattatcc attagacagc tcgaaatgaa tttcgggtat
     5041 tcgaatggat atttaggaag ttggaaaaga caaactccta attctattga gttagctcgg
     5101 ttagctgatt actttggagt ctctgtagac taccttctag gtagagaaga aagagaaacc
     5161 cctaaacatg tggatttatc agaaaacgat actgtttttt cttttgatgg aaaagaaata
     5221 tctaaggaga caatgcgtaa agcgattgca attgctaaag ctttagagga aaatgaatag
     5281 ttggagtgat gggttgtatg tatttgaaat tgaaagaaat gttgagcgaa tacaatttga
     5341 aagtagttta catggaaatg aaagagcctg gcttctatta tccgaagcct agaatcattt
     5401 ttttgaatga aaatctatat ggcgaaactg cagaagcctt tcatttatct catgaacttg
     5461 cgcatttcac cgcttctcat tttgaatatt cagttttgta cgatacttct acaacttttc
     5521 actcaaaatt tgaaacagaa gcagataaaa tagctattct aattttatta aatatctata
     5581 ttgagaacga actgactgat gaatctcagt ttaatctcga aaaatttatg gagtattatt
     5641 ctattcagaa taagctcaga tacacttgtt atgctgtctg ccaatgctat tttaagaaaa
     5701 aatattctta cgcaaggcaa tatgtatgaa tacatcaaga attattaaaa aaaccgattt
     5761 cagcaaaaca ttaaaaaagc ccgtgctggc acacggactc atacctcatt tctgagatcg
     5821 caaatatatt ataacaagaa gtgaggaata tttaaatggc aaaaaaagtt atgggtcaag
     5881 acggaaaaat gtataaagtt agtaaacctt tttacaaaaa agtatggttt tgggtattag
     5941 cagtaatttt aattataatt attggttctg ctttaaatgg tggatcagat agtaataaag
     6001 caagtgataa tggtggcgaa aaagtaacta aatcttcaac ctctgcttca tcttctaaag
     6061 aagaaaaaag cgataccttc tataagattg gtgacactgt aaaagttggt gatgctgaat
     6121 atacactaaa tagtgtagaa ctaactgatg aaagaaatca atttgaagaa aaccaacctg
     6181 cacaagtagt aaaaattact tataccgtaa aaaatgatgg cgattcagat atccctgtag
     6241 gtacagatgt ggaagtatat ggaccagatg acaaaaaatc agagacatat gccaacgaaa
     6301 atacaatggg atctgttgct ccaggaaaac aaatggatgt aactgctcat ttcacgttaa
     6361 accaaaaggg agaaatagaa atccacttct ctcctttagt atcatttgaa aaagcagcta
     6421 tttttaaagc aactgtataa taaataaaaa acacgcccca ccgtccagag taagagcgtg
     6481 cttttagaaa acaaacctat ataggtctat ttgttatgcc tattatagca aatgatagga
     6541 gatgttaaaa gtgtgggttg aacagaccaa agatggccaa ttcaagttta ttgaaagata
     6601 tgtagatcct tatacagaaa aaacacgaaa aaaatctaca acacttacaa gtaattcacc
     6661 acaagcgtgg aaaaaagctc agaaaattct agataaaaaa attaaagaag cactcgaaga
     6721 ttacaataaa tcagatatca cttttggtga gctgtataaa gaatggtatg aatattataa
     6781 gcagcatgtt aaacgtacta gctatttgaa ggttccaatg atgatgaaac atgtttctaa
     6841 gcacataagc gatgatacga tcgttagaaa cattgatgag acactcatta ataagataat
     6901 tgaagatatg tatacgtttg gtgacctctc actgaactac acaaaacaaa caaaaacaac
     6961 tctatctgtt atgctaaact atgcaatcga tagaaaatac attcaaagaa accctgcgct
     7021 agcagttaaa atccatccta aaaaagtgga agaagaaaaa agaaggcttt ctatgaataa
     7081 aaaatatctg gagaaagaag aaattgatca aatactgaaa cagctatact ccaatcctcg
     7141 cagaaaacta cacggcataa tagctgagtt tctatattta actggtttga gatatgggga
     7201 attgctagct ttgcaaatga aggactatga ggatgggaaa atttccatta atgggacctt
     7261 agattacaca tctgtgaaaa tggataatgc tataaaaaca actccaaaaa atacttattc
     7321 gcaacgtgaa gtgcaattac ccaatcgtgc aaaagaattg attgaaagtg tgatagctga
     7381 caatattctt gcaggtagac ccacagatcc cgatcaatat atatttatat ctacaagtgg
     7441 cactccgctt gcgctgcact catttaacgc tatactccat aaagtagagg aagagttgga
     7501 attagaaaaa agtctatcct cacatatatt cagacatagc cacgtttcac tattatctga
     7561 attaggcgta cctcttaaag ccattatgga gcgtgtaggg cattctgatg caaacacaac
     7621 tctgtctatt tataaccatg taacaaaaag agccaaacaa caagtaattg ataaactaaa
     7681 tagcctttga tatttttgcc ccttctttgc cccttttgct taaaataaaa caaaaaaaga
     7741 agaaagaacc ctgttaaaat agggttcttt cttaattact acatcatacc gcccatcatt
     7801 gatggatcca ttgcaggagc tgctggcgct gctggttctg gtttatctgc aacgactgct
     7861 tctgtcgtta ataacaaggc agaaacggaa gcggcatttt gcaaggcaga acgtgttact
     7921 ttagttgggt ctacgatacc tgcttcaacc atgtttaccc attctccagt agcagcattg
     7981 aaaccagtgc ctagttccac gttcttcaat ttatcaacaa tcactgatcc ttcataacca
     8041 gcgttttcag cgatttgacg aattggttct tctaaagcac gaacaacgat cttgatacct
     8101 gttgccacgt ctccttctgc ttctacagca gaaactttgc tgataacgtt cactagagct
     8161 gtaccaccac ctgagaccat tccttcttct actgcggcac gtgtagcatt caatgcatct
     8221 tcaatgcgta attttaattc tttcaattct gtttcagtag ctgcaccaac tttaacaaca
     8281 gctacaccac ctgctaattt agctaagcgt tcttgtaatt tttcgcggtc aaaatcagaa
     8341 gttgtttcag caatttggtt tttgatcaat tgcacacgag cttcgattgc ttctttttct
     8401 ccagaacctt ctacgatcgt tgtattgtct ttatctacaa caactttaga agcatttcct
     8461 aagttttcga ttgttgcatc tttcaattct aaacctagat cgtcagtaat tactgtacca
     8521 cctgtcaaaa tagcgatatc ttcaagcatt gctttacgac gatcgccgaa accaggagct
     8581 tttacagcta ctacgttgaa tgttccacgg attttgttca atacaagagt tggcaatgct
     8641 tcaccatcta catcgtcagc aataatcaac aatggacgtg attgttgcaa gatttgttct
     8701 aacaatggca gaatatcttg gatgttagag atttttttgt ctgtgatcaa gatatatggg
     8761 ttttctagaa cagcttccat tttgtcgtta tctgttacca tatattgtga taagtaacca
     8821 cgatcaaact gcataccttc tacaacatct agttctgttt cgatcccttt tgattcttcg
     8881 attgtgatga caccgtcgtt tccaactttt tccattgcat cagcaatcaa atgtccaact
     8941 ttatcagaac ccgaagaaac agctgctact tgtgcaatgg cttcttttga atcaacaaca
     9001 gttgagatat tatgcaattc ttcgactgct gcttttgttg ccaattcgat cccgcgacga
     9061 atgcctagag gattagcacc tgcagttacg ttttttaaac cttcgcgaac gattgcttgt
     9121 gtcagaacag ttgctgtcgt tgtaccgtct cctgcaatat catttgtttt tgatgctact
     9181 tctgatacaa gttttgcacc catgttttca aaatgatctt ctaattcgat ttcttttgcg
     9241 atcgtcacac catcatttgt gatcaatgga gagccgtaag atttttctag tacgacattt
     9301 cggcctttag gtcctaatgt tactttcact gtatctgcta atttatctac cccgcgtaac
     9361 atagctgcgc gtgcgtcttc tgcaaatttc aattcttttg ccatgattga ttcacctcat
     9421 tgattatttt tgattatttt tttatcttcc aaatttactc aacaatcgcc atgatatctt
     9481 ttcctgcgac gatcagatat tcttttcctt cgtattttac ttctgtaccg gcgtattttt
     9541 cgaacataac ttggtcgcct gcttttacag cggcaggaac tttttcaccg ttttctaaca
     9601 aacggccttc accgactgca acaactgtcc cagtttgggg tttctcttta gcagcagaag
     9661 ctaaaacgat tcctccgaca gttttttctt cttcttttgc gacttcgata atgacgcgat
     9721 cacctaatgg ttttaacacg ataaatccct ccgataactc tttttttaat ctgttagcac
     9781 tcttaacctt cgagtgctaa cctacatttt taataatact cattctccct cttttttgca
     9841 agcttttaaa tcaaagaaaa gaaattttat tctgacagct gcttattgaa attatgatat
     9901 aattgaaatc aatcgatata agcttgcctt gcatgcttgt tatcccttaa aaggaggaat
     9961 tacatgtctt taaaaaaata tagcttcctc agcatttttt gctatggtct aatttttatc
    10021 tcacccttga tcctagcttc gataggactt gtaaaatcta cgtctgaatt gatcacagca
    10081 actgcagtca tgtatatact aggcgccgtt gtcctagcta ttttttattt taagcaacgt
    10141 gaacccttag cgatcgaatc agcggtaaaa aaagcttctg ctccaaagat tgttatttat
    10201 ggattggttg gaatctttgt cgcattgatt ctgcaaagca tcgctgtcat gttagaatct
    10261 gtattgtttg gcaaagcaac cccttccgag aatacacaaa atatcatcca gatgatcatg
    10321 gaagcacccg cttttatttt ggcgacaact gttgctggac ccatcatgga agagttcgta
    10381 ttccgccgca gtatactggg gatcatcagt cgatattcaa atttttgggt cggagcagtg
    10441 atcagctctt tgctgtttgc gtttgctcat aatgatggtc atctgctaat ctactttttc
    10501 ctaggtttct tcttcagcct agagtacaaa gcaactggtc ggatctggac atcgatgatc
    10561 acacacgtag gaatgaacac attagttgtt cttgttcaat tagctgtcca aaaagggcta
    10621 gtataaacat gactataaat actcctgtca tcctagattg gtgattttcc caatctaaag
    10681 cgacaggagt atttttgttt tttagtcgtt catcttttct gctgctccat cattttttgt
    10741 ctgtctttcc tttttgcagt ttgcagaatc tatttttttc aaaaatggga aataaatgaa
    10801 aaaactaatt gacaacacga taccttgtaa aatcgctgca ggcactccgc ctactaagaa
    10861 acccgagagg atcggcggtg ttgtccaagg gacttggact gctgtaaata aaggaacgaa
    10921 tccaaaatat aaagcggaat aggtaatcaa tcctgaaacg accggtgcga aaataaaagg
    10981 aacagccatc atcgggttca tcacgatcgg tgtggaaaaa ataattgatt cattgatgtt
    11041 gaaaaaggct ggtaaaagag caagatttcc taattctctg tatttcttcg atttcgcaaa
    11101 aaccgtcata aaaaatacag cccctaacgt caatccagca cccgtaaccg tcatgaactg
    11161 atcgagaaat tgctgagtaa cgatatgtcc gccatttgcc accgtcaact cttgtcctga
    11221 ggctaagatt tctgcatttg ctaatgaatt cgcttgcaag acgggactaa ccaacccatt
    11281 gaccatcgca gatccatgaa gaccaaataa ccataaaaac ggaacaaaaa aacctaataa
    11341 aagcacacca aacaacgtgt ctatgaacga ttgaatggga agctggacat atgtccatac
    11401 tccttgaaca acagtcattc tgaacaaatg ttgaaaaacc gctgaaccga taagcgtaaa
    11461 agtgactata acaatgattg gaatcaatga agtaaaagac tgccaaacat tttcaggtat
    11521 cttctctctt tgcttccacc ctttcgcaag tcttactaaa cgtgcatagc cccatccgat
    11581 gatgagccca gttgtaactg ctcctagtat cccttcagca cctgtccaga cagtatctat
    11641 ctgagtattt agtggctgaa taagcagtag ataagcagac aaggctgtta tgccgactgg
    11701 taagcgatcg atgtcttctt cttttgcata agcgtatgca atggcaaaaa cgatccaaaa
    11761 ggctatcaca tcaaatgtag cctggtatac ttgtgtaaaa tacatagaaa atcctgtttt
    11821 gtctatccac tgagcgatgt aatccacagg aaaatgagca aataagaaaa acagcgagcc
    11881 aacaataatt attgggatcg tgtaagtcat tccattacgc aaagcagtta agggtctaag
    11941 attcacaaca gatgttatca ttggtaaaaa ggcacgatct aaccactctt tcatttctta
    12001 tctccttcaa cttctatttt tatcaatcag ttttcttaat ttaactatac ccaatgatca
    12061 cgaaccatga aaggaatgcg cactgagctt ttcaaaaatc ggattctttg acgaatattc
    12121 agcaactgct tacaatggac aggaataaaa aaggaggttt tttatgaaaa ttgcactcat
    12181 tggagctact ggccatgcag gttcatttat tttagacgaa gcattgtcca gagatttaga
    12241 cgtgacggca atcgtgcgtc atccagaaaa attgccatca gatgttccgt ttatacaaaa
    12301 ggatttattt gagttgacaa ctactgatct tgcatctttt gatgttgtcg ttgatgcttt
    12361 caacgctccc aaaggaaaag aagaaatgca tcaaacttct ttgtcccatt tgattgagat
    12421 gcttgaagga accgatacac gcttaattgt cgttgggggt gcctcttcgt tattcttaga
    12481 tacagaaaag acgactcgta tgattgacca agtacctaaa aatgcaccct tctatccaac
    12541 agctttcaat atgtacgaag gattgttaaa attgaaacaa tctaaaaatc tacattggac
    12601 ttatatcagt ccagcttctt tatttgatcc acatggcaaa cgtacaggta cttatttgtt
    12661 aagtgatgat tatttacaaa gaaacgctgc tagcgacagt acgatcagca tggcagatta
    12721 cgcgattgca ttagtagatg agatactgga tgagaaacat gaaaagcagc acatcagtgt
    12781 agtcagtcga taatacggtt atttttttaa tatgctaaaa aaataaaaat agaagataaa
    12841 accaaccctg attgatttta tcttctgctt tttatatttt tattgattga actgtttaaa
    12901 tcgaaaatag ctcagttgct tcatctggat ctgtatcata gatttcaaaa tcatgtccaa
    12961 cgccaaaatc tttttctttc aaaacatttt ccattgttaa atgagcaagt tctttcatat
    13021 tgttttcctt gctaagatgg ccaagataga ttcgtttggt ccgattaccg atcacatcag
    13081 tcatgacaag tgcaccgtct tcgttggaaa gatgtcctct atctcctaaa atacgctgtt
    13141 ttaaattcca cggataaggt cccatgcgta acatctcaag atcatgatta ctttccatca
    13201 aataaccatc ggcattttca atgacacctc gcatataatc actgcaatat ccggtgtccg
    13261 tcaacatgac gaatgaccga ttttctttat ggaaacgata aaattgcgga gccgctgcgt
    13321 catgggagac gccgaaactc tcaatatcga tatcgccgaa tgttctgact ttccccatct
    13381 caaatagatg tttttgttcc actggtacat ttccaatcag tggttccatc gcttcccaag
    13441 tctgctgatt ggcaaaaaca tccaaatgat acttacgagc caagacaccc acgccatgta
    13501 tatgatctcg atgttcatgc gtcacaagta ttccatctaa ttggtccggt gtccgatcaa
    13561 tttgagccat caaggaagtg atttttttac cgctaagtcc tgcatcgacc aaaagccgtt
    13621 ttttttcact ttcgatataa agtgaattac ccgaacttcc acttgctagg atactgattt
    13681 taaaagctgg tttttcttca gccatttgac tcctacttcc cttcttactt tgtaaacttt
    13741 tttcattata ctcgcttttt tattgatctt ccacttttgg tacagtattg tttgtcacga
    13801 tcgtattgct gaaagcgttg acgctctcaa tttggaccac tttatcgctt gtttcgatcg
    13861 ctacatgcca aacaggaaca tacacatttt tttcccggac tttcagaata caggaataag
    13921 ctagctgacg ccataaaatt tttgatcctc ttggtatttt gttattgatg tacaatgtat
    13981 tgattgcttc ctcttccgta tacagttccg tcttttcgcg cagctgctcg atatcagata
    14041 aatgcgtctg catgtatttc gtgatgctat aatcattatc gtttttttct aaagaaagaa
    14101 cgatttttgc tgtatcatca ttgaatggga tgccttcata ctcttggctt gcttggattt
    14161 ctagtggttc ttcttctaaa tgagaaaagt ttttcgtgta cacataatca ttaccgaata
    14221 aaatattgtc tttgttagat aaaaaatctt ccaaggaact actaatttta tctggatcga
    14281 tcacgttctt ttcagcatct gaactggata aagatttcgt caaaacatca ccgtctaaaa
    14341 acacaccact tgccagcaag ccgcttttac cagccttgcg ataattcgct aatgaagtgc
    14401 taagattggt ctgctccgcg ctaagataat agccttgctt gttctcagac gaaatcttcc
    14461 ccgtgtaagt gatatcgtcg ctagccagac gttgttggat tgattcagtc tgattagagc
    14521 gaatgacatt gttttcttgg tataccgcgt catgataact gctgaataaa aacacattca
    14581 accctaaaaa agcgacgaaa aatatccatt cgattttttt gaaatccaat taattcgcct
    14641 ccaatccagg taatttttcc agaaggtcgt tagctgaata ccagttatct ttatatttca
    14701 cataccattc aggtaagaga tcaacgacac gattcgtttc tttgacattt tgccatgtat
    14761 agccaacgat cactgaacca atcttgttgc tatctgctcc cttagctttt aactgctgga
    14821 tgatttcatt cgtacttgca agttctactt cttcgtctga aggtatcggc acttgaatcg
    14881 tattcaaact ggtttggata ttcacttgca cagaaccatt cgtttcaccg ccaacttcca
    14941 caccaatctg tcctctgctg tctgtcccaa aaaccgggaa gccttccacg aatatccgat
    15001 aatcgatcgt attgtcactg cggtcaaaat atctgaggtt ccctaaagaa ctgcctaatt
    15061 tgctaatata ggggaaactt tcagaaaata cactagcatt ttcttttttc tctggcagct
    15121 ctccacgaaa atcaactact tgcgtttccg aatggataga cattgtttca ttcccaccgt
    15181 aaaaaatcaa ttcattgctt tcatcattat ttttcacgct atctggtttc tggaaaaaag
    15241 catttctaaa aagtgtatag ggttgctgtg acaggatata actatacttc ttcagtttga
    15301 tcgaatgttt cgtagcatat tgcgtgtcga tcgtcgatgg ttctgttgtc atttctgtcc
    15361 attttgtgtc ttttgctgtt aattctttat tgatatctgt ccaattcaac gaaatatttg
    15421 cttcatatac gagatggttc gaataattca aaaatcggat ttttttattt ccttgatcga
    15481 attgtattct agtaaaatac atattttcgc tttcttttgc caagttcgac acatctaaat
    15541 ctaaatcaaa tgcttccttg tattctctta gcaaaaaagg cgcattatag gccaactcaa
    15601 aaccagagtt cagatttttc acctgttcaa actgtgattt gtctcctgaa actacttcag
    15661 aaagctttcc aaatcttgct ttatcgatga tcaattgcaa tcggtacacg agattttcac
    15721 tgttggtttc ttggatcatt cccgaatggt gccatgtaac atgaagcgga agaaagactt
    15781 cggaagtttt tcgattgctt tgagactcga tcatttgagt gtttgtctca tctacgttga
    15841 ttgaactttt tcctgctggg ctcagccaga ttgcataaga gaagtatatg cttaacgcaa
    15901 ccatcaaaat gagtcctata cgaacgactt tttcagagat tctcattccc accattcctc
    15961 ctcataaggt tcatatggca gcgagatata aaaagtcgac ccttttcctt ccttgctctc
    16021 tgcccaaata gcgcctcgat gcgccttaat cacttctcta gaaatagcta atccaagtcc
    16081 tgtcccacct tgtgctcttg cacgtgcttt atctactcga tagaaccgct cgaacactcg
    16141 gttcagatct tttttaggaa tccccaatcc ttgatcggtg atacttaaaa tcacgttatt
    16201 atgcgtttct aataagcgac aagtaatcgt tcccccatct ggtgaatact tgatcgcgtt
    16261 attcatgata ttatcgacta cttggatgat tttatctgta tctacttcga cccaaagatc
    16321 tctttgcgta aattcacgac ggatcttata atttttttcc tgatttccca ccatcatatc
    16381 aaaccggtca agaacaaaat tcactaactc gttgaaattg acatattcta attgaagctg
    16441 ggtattacct gtatccatgc gagatagatt taagaggtca ttgatcattc gaatcattcg
    16501 gtctgtttca tctagcgtta ccttcaaaaa atttggtgct acttcctcgt ctttccacgc
    16561 accttcgctc aatgcttcga tataactacg catacttgtc aacggtgtac gaagttcatg
    16621 ggaaacatta gagacgaatt cacgacgttc ccgttctgtt ttttcttgtt ctgtcacatc
    16681 atgcaagaca gctactaatc cgctgataaa acctgattcg cgacggatca tagaaaagtc
    16741 tactcgcaag atcatctgat cactttccaa tgtactggtt gaacggtcaa gtaccatttc
    16801 gtttggatct tctaataatt tccgcaacgt atattcttct tcgatatcta aaagtgtcaa
    16861 aatcgattgt ccgattgctt cctcgttttt gatatctagc aaggaaacag ccatatcatt
    16921 gatcgtaatg actttacccc tgcgatctgt cgcaatgacg ccgtctgtca tatgggatag
    16981 cacgctatcc aaacgattgc gttcagcttc cattgtatct tgagcttctt caatacgttc
    17041 agctaactga ttgaaggtat cagccagctg tccaagttca tcttgtccat gcactttgac
    17101 tttccgactg tagtctcctc tagcgattcg aatggcttgt tctctcattt caccgatcgg
    17161 ctgtgtaatc gaacgagcaa ctaaaacaga tacgatcatt gaaataaatg ccgcaattag
    17221 tgaggcagta aagaaaatga ctgctgtgtt attgatttcc gaatattttt gttcgatatt
    17281 acttttgacg taaagagccc cgataacggc atcgccggtc ggggacagga tcggttgaac
    17341 attgatattc acccgtttat cgtcgttatc taaagcaaca taacgtttag tcgtgaaatc
    17401 attgatatca acataatcat ttttctttcc cacagcgctt tgttcattga catcggttgt
    17461 tcctcggatg atcccttttt cgtcaacgac ccgtatttcg atgatatcgc tagtcgcact
    17521 gttttcaata aatcttttga tattcgcatt tacctcttca tcatctgcat tatcttttcg
    17581 attcaattcc gggctgattg ttgttgctaa tgattccacc gtttgattca tatctttaat
    17641 aaatgtattg atcgtggaac gttctaaacc acgtataaag taggcaccaa taatttcgat
    17701 cgagatcaat aaaatcagaa taaaggtaat agcgatctta aaattgactg atcgaaaaaa
    17761 gcggactttt cttttcatcc aattactcct gttctgggtt acgcaaatag taaccgacac
    17821 cacgacgtgt cactaaataa tttggatggc ttggattatc ttcgatcttt tcacgcaaac
    17881 gacgtacagt tacatccact gttcgtacat ctccaaaata atcataaccc caaaccgttt
    17941 gtaataaatg ttctcttgtc atcacttgtc caaggtgctt cgccaagtaa aagagtagtt
    18001 cgaattcacg atgtgttaac tcgattgttt ctcctcgttt cgtcaccatg taagcttctg
    18061 gatggatcgt caaatcacca atcgtcaatt ccgcaggtgc tgcctcttct ggttccttcg
    18121 cagccgtggc accgcgacga agatttgctt tgacacgtgc aactaattct cggttggaaa
    18181 atggcttggt cacgtaatca tcggcgccta actctaagcc taggacttta tcaatttcag
    18241 aatctttggc tgttaccata atgatcggca tatcataagt tttgcgtacc tcacgagcaa
    18301 cttctaagcc gtccatttta ggtagcatca aatcaagtaa aatcaaatca ggttctacct
    18361 cagcaacttt ttccaaggct tcttccccat catatgcagt ataaacgtca tagccttctt
    18421 tggctaaatt gaatttgacg atatccgaaa tcggcttctc atcatcgaca actaaaactt
    18481 ttttcataaa gagccacctc ttatatatta ttgtcatacc ttctcattat accgtatttc
    18541 tgcttttgtt tcatctttgc ctataaaaca aaagagaaaa ttaaaatgaa agtgcaaaat
    18601 actttattat tttttgttct aacttcaata aaaaaggaaa acactgattt tcacctcagt
    18661 gtttcctttt tttgatttat ccttctaaaa cttgtacacc gacacccaat aatcccggac
    18721 cagtgtggac tactaatgcc ggagaaatct ggccaaagta gatttggttt gcttgtggga
    18781 actctctttt aagtctcgct tccatttcaa ttgcttcttc tttcgcatcc ccatgggcaa
    18841 cagctaagtt gaagaccttt gcattaccga ttttttcttt gacgtaagca accgttttat
    18901 ccaaactttt tttacgtccc ctagcttttc ctactgtgta ataaatccct tcttcattac
    18961 atgagatgat tgggttaagc tttaaagcgg ttcctacaat agaagcaact aaaccgatcc
    19021 gtccaccttt ttgaagatat tccaatgtcg caacattaaa gaaaactttt gttctttcga
    19081 ctaactcatt cagattactg atcagttgat cccattctac tccttcttcc agccattttg
    19141 ctgcttggat cgcagaaaaa cctgcaccaa taccaatatt tttcgtatct agtacatatg
    19201 cttccaattc ttcttgctgt tgtgcaatta aacgaaccac attatatgtc ccgctcaatc
    19261 cactggaaat tgttaccgca agtacctttt cataaccgtc ttttttgatt tgctcaaaga
    19321 tttctgtgat cgactcacca tccggcaaag atgtaccagg gatttcctct ggcaaacgtt
    19381 gatagatctc ttcaggggta atgtcaattt tatctgtata tacctgatct ttataaataa
    19441 ttttcaatgg gatcatatac atctcgtatt tttttatcaa ttcttctgga acatccgtac
    19501 ctgaatctac taaaattgcg attttattgt ttttcatttg ttttctcctc ttttaaatat
    19561 tcggtttcta gaacaatcgt tttttccgca agaagtttca ttgcaaacga taaagttgcc
    19621 gctcttacag caatatattc tacaggggtt gcctggtcga atttttgcgg atgagattta
    19681 ccttgtgcta attggctgac catccacaca gctgcttctt gttcatcgca aaatagatta
    19741 taggcttcac gaattcctac tgtttttcct tggaacaata tcccttcttt gatttcagga
    19801 atagtcaata cttgtttcaa cgtagtgatt gcaagtaaaa atgctacgtg ttctttgtta
    19861 tatctttttt taactggtgc tggtatcaaa cctagtttaa cgtaattgtt taccatagaa
    19921 gaggtaagca atggatgctt ttctccttgt atcacaggtg aaagataacg atcaactaac
    19981 gttctgactt ggtccatata aagttctaag tcagggagtt cttcccagtg aggcagacgg
    20041 aatgtttcta gttccttccc ccactccatt aattgctgac gcattttttc catcataacc
    20101 gctccttttt tcatttcata aacatgatat cagacttatg ataaacattc aatctagttg
    20161 ctttaactag gtgatttgat tagcgggatt ccatgaaaac aaaaaacaag aaggctaagc
    20221 cttcttgtct tttatcgtta agaacaggag acccgccggg gagacggcgg gaaaggagta
    20281 aaaaatgaaa aaagtgttag ggtttgttct cttgcaagaa ctttgtttgt tggtatgctt
    20341 atataatagc gagcaaatgt gaataatcta tgaatgattt cctataagga agtaaagatt
    20401 tttctgattt tctttaaatg tagtcttgca ttttaaaatt cttagtgcta ctattattaa
    20461 gtactttttg tacgggccgt tagctcagtt ggtagagcag ctgactctta accaaatgct
    20521 ctatttgtga aaaaagtttt ataaagcttt ataaataggt ttttagtttg tgaatttttt
    20581 cattgccaac atactgccaa caaattgcca acaaaaaaat tctaaaaaca agaagcgata
    20641 ccccaaagat attgtttttt gtttttttat tctttaaacc caaatgcgtt ttctaatgct
    20701 tctgctgcac gatcttttac tcctgcggtt aaatgcgtgt ataaattagc agttgtcgaa
    20761 tacgatgcat gtcccagcca ttcttgaatg gatttcatat tctggccact atctaacatt
    20821 attgaagcat tagaatgacg caagtcatga aagcgaattt ttttcaatcc atttttctta
    20881 atcaacttac tcaaacgatg cgttacatat tgaggcttca tcaaccgtcc atcttccata
    20941 gtaaatacgt actcattatc ttctaaaata taatcatctc ctaaaagaat acgattatct
    21001 tgttgccatt tagcttgatt tactaatgct cgttcaatcg tttcaaccaa aggtaatgta
    21061 cgatagctgg aagagttctt tgtttgatct ttctttatta actgtgacat attctctgga
    21121 ttttctatga ttacatggtt tatcatgatt tttttgttta aaaaatcaat agcagaccat
    21181 tttaatccta aaacttcact tcttcgcaat ccataaaatg ctgttaaaag aactaccaaa
    21241 tagagttttt cttctgacaa aatgtcaagt aatttcaaga tctcttgttg attataaact
    21301 tgagaaacaa attgtttctt ttttggcttt cttactttat ctgctacatt tgtacttatc
    21361 aattctgtaa taacagcctc ttgaagagct ttgcgtataa atgcgtgata atgaataacc
    21421 gtatttgctg tacagttatc atttaacata tattgataaa actcttgtaa atgataaggc
    21481 tttaacctat ttagtttaat ttttttagga taaaaaaact tgtaaattcg attatggacg
    21541 taatgcttat acccaacata tgtgttaaat gctacttgtt tttttgatac ttccaaataa
    21601 ttaagcaaat agtcataaaa caataaattt ggattagcac attcacattc catagctaat
    21661 tgactttcca attctagtaa aaattcatct ctaatttttt ctgctcgttt tttatttcct
    21721 ttctttttta attttgtatc tttccacttt tgtctaattt ttccgttaga gtctttataa
    21781 gatacaacta cttgtaatgt tcctcttttt tcaattatgt ttgctcttaa tttcaattca
    21841 atctctcctc ttaaactgaa atatcggcaa aaattttgac tattttatta tagcatgatt
    21901 tttaccttca tcttgtattt tcaatatata ctcaatgaca gcttttttag gtattttgta
    21961 ccttgctcca atttttatat tttctattga tctatctttc agcaaattat aagctttatt
    22021 ttttccaata tttaacattt tagttaaatc agtaattttc aatatcgatg gataactttc
    22081 aaatattttt tcataatctt gaaaatccat aaaattcact ctcaattctg ataataagta
    22141 ttgcactttt ttattttagg tgttagaata acaaagtatt aatttgggct gttagctcag
    22201 ttggtagagc agatgactct taatcatcgg gtcgcgggtt cgagcccctc acagcccatt
    22261 gggtgccaaa cccttaaata cagtgtaaac gttggtgctt agcatttaga aaacgtatat
    22321 actgtatttt tttgttttca aaagagactg tagtacccct ttttttcact aaaagcccac
    22381 tgtagtcatt gatatttttg ataagaaagg aacataatta tgcgtaatga aattgctaat
    22441 ataacaataa tccgagaaac tttacaaaac catactgcaa atgagatctc taaacataca
    22501 ggattaaatt tgagtactat aaaaaaacta aaaagtggag aacgttcaat agaaaaacta
    22561 aatttacatg atgctatttg cctcactgaa tttggtctta aaaataatag aaaaaatgtt
    22621 gaaatcaaca tatggaaata attcttaatc agctgctttt gtcaaaagca cataggaatt
    22681 tcacctctct ccaagtttac ggcgagcctt acggaagtat cattatccca acttcaaatc
    22741 atcgcttttt attgactata aaaattattt tagaatgttt atcgctcctt ctttacagta
    22801 agaaatcatg gcgcaactaa aatatgctcc ttgaagtgtt aacgtactga tagaatgtat
    22861 ttaaatttca atgatcaata ggttttctgt ttaaaagaat ctactaatcc tttaactatt
    22921 agaagattct tttaaacaga ataagtggaa aggggacttt ttagttagtt aattttaaat
    22981 tttaagatcg cttctatcaa tttaatttct agctgtctta gcatatactc atctacatag
    23041 gtaataaatt gccccgcctc attaaatcca tttgtcaaac ataacttagc tatataacta
    23101 tgataatgtt tgagtatcat cttgattgaa attggatctt cttctagagc agattggata
    23161 atggatagag gaactaatgg atactgccgt tttttggggt tgtctaccat aagtcatacc
    23221 ccatttttaa ttgttcaact gccttctttc gttgatacca gataccactt gtactcatat
    23281 tgaggatttt accgatttca gtatcattaa aacctgcaaa ataataaagt aggataacct
    23341 ttcttcttat ttccgataaa tgatcaatgg ctgccgataa cttttgatct gaaattaata
    23401 tttccattcc aaataagaaa tagacatctt gttcatcaaa atgagtttgg gagtacttac
    23461 cttcatttga attcgtcaat ggttcctcat tatctgttat gttaatgttt cgttttcgaa
    23521 tagacttagt ttcgtttcgt aatacttttt tacagtaggc atcaaattga tgttgtatag
    23581 atttctcatt gtaattttgt tgcatgataa atatgctcct tttttcccct ttccactaat
    23641 acaacaatca gaaagcgcct ttttttcgaa aaaactagaa aaaaatatcc ttctttaaac
    23701 agaaaatctt tttataaaaa aaaggagttg gcaattaagc agccactcct tttttatatt
    23761 ccttataaaa ggtaaattat tttttataac tgaaatttgg tattgatgac catcgtttct
    23821 ttagatagtg agcaatcatt ttaggctttc tatctctagt aaagattcct ttcttatttc
    23881 cttgcactct taaaatccct tgacttgttt caaaatctgc aaaattccac acttgttcac
    23941 caacaaaatt gtcacattta tctattactt cgtgatttgc ttcgtaataa cccacttgat
    24001 actcttctgt aaacattgtt ggtactgtat catgaagtcc agctactgta tctgctccat
    24061 attctgtaaa cattattggc gtatcgggac atctttccat ccatccatct agctcttttt
    24121 tcaacagata ttttgcatgt tttaggtctc cacctgaaac ataccaacca taatagcgat
    24181 ttaaacataa aacatcgaca atatctccta ctttacaaat atctggtgtt gactttaagt
    24241 aagtaacgat tgttacaggt ctcttctgag ggtcaagttc tttagtcaat tcaactaaag
    24301 gttcaaaata ttctttagcg ccttcagaat ccgaatctgg ttcatttgca atagaccaca
    24361 ttacgacact cggatggttc ttgtcgcgtt ctatcaattc cttaatcgcc attttgtgag
    24421 cattttctgt ccgtatttct ttccaagtat catgtttttc tacgttgtca tctaataaag
    24481 tagccatgaa attcaaatgt aatcctactg caggtacttc atcaataact acaatacctt
    24541 ctcgatctgc taagcgcata atttcttctg aataaggata atgtgaagtc ctaaatgaat
    24601 tagcaccgat ccatttcatt aaattaaaat ctttgatatt tactgcttca ttcaaaccac
    24661 gaccattaat atacgtgtcc tcatgcttcc caaatccctt aaagtaaaaa ggtttattat
    24721 taatcaaaaa cttcccattc gaaacctgaa tactacgaat gccaaattct tctgtgtacg
    24781 tatcaatcac tttattttcg ttaattaaac taactttcat actatataag taagcattta
    24841 gaggctccca aagtgttggg ttcttaagtt gaatcactcc ttctttcccc ttagcttctg
    24901 ctataagttg agcttcttcg tcatagattg ctattagaat ttctccggtc cattctgtat
    24961 cgatattaaa ttctacattt gcatctgaat caattatttt tgtattaata gtgacatcct
    25021 ttatatagtc tttcggagtt gtatacaatt taacgggtct attaatgcct gcataattga
    25081 aaaagtcaaa attaaccaaa ttttcttttt ttatgtctcc atcttcctgt atcgtttctt
    25141 tcaaaatccc tacaggtaaa gtgctatcgt caataacatt gtttacagcc actgtaattc
    25201 tgttacttcc atcttttata aactgattaa tttctgattc aaatggcgta aaccctcctt
    25261 tgtgttgatt aatcaatttt ccattcaaaa atacttttgc ttcatgtgtt gcagatccaa
    25321 aacgaagaac taatctttct tctaataatt ttttaggaag aatcacttct ttttcatacc
    25381 acacccatcc aacatgattt ctaatgttct ctgtttcaaa taaatcatta taagaagaag
    25441 gaactgaaat tggataactc tctttttcaa ccattggttc taaagcaagt tcttcttgta
    25501 agtcatcatt tgtatctaac ttaaatctcc aaagaccgct taaatcaatt acttgcctcg
    25561 tctctgtcat aatgggatat aacatgtaaa tcactcctat ataatttaaa tattaaataa
    25621 atgtatcctt taacccacaa taagcatcta ttggacttat tcccttaaat tcagaaaggc
    25681 ccattagttt gtccactaat atttctaaag tttcttgttt tccatcataa gtattgatat
    25741 aagtttttac ttgaggcaca tcagctaaat gaaacggaca ctgtacagat acaaaaattg
    25801 ttggaatttc atgaatataa aatggaatat ccggagtacc tttacttgct ggccaaacaa
    25861 ttctttgaac tgtacctggg ttaacattag ctatattaat aatcaagtca tatttttcag
    25921 ttaaatcttt gattggtctc ttagcagcat atattccagc tatattttta attttttctt
    25981 cttgcgataa tcctttcaat tcttcttcag gtgaaatgta tttttcaact tcaaatcctt
    26041 ttgctgacaa aatctgtttc aataaatcaa tggcctgagg tctatttcca gcaactactt
    26101 caccaaaccc accttttacc ccatgtacat ctaccagtaa aatcctttta tatttttctg
    26161 gtacaatagg ccaaatatct tgtttatctt taactagtgt aattgcttga tcagctacct
    26221 ctttaaaaat tgatttattt tcaattaaac caattttttc catagcttct tgtttcggta
    26281 acaaaatttt attcttttct tttttatgca aacctagttt agcctttaag cctaaaattc
    26341 ttcttaatgc atcacgtaaa cgctcttctg aaataattcc tttgtaataa ccttccatca
    26401 tataattaaa gtcttcatct ggatcattaa aaaataggaa tagatcacat ccagccgcaa
    26461 tagaagtggg caataattct gaccgtttca ttgccccagt cattcctaac atatgactag
    26521 catcagtaac tactaatcca ttaaacccga gttttcctct aagtaagtca gtgattaatt
    26581 cttttgataa agttgcagga agtaaatctt catctttcag atcaagatta aaatgttttg
    26641 aataagctgg catatggata tgacctgcca taattgaagg taatccagct tctattaagc
    26701 ctgagtatac ttttccaaaa gaattatccc attcttctgt acttaaacta ttaatactaa
    26761 aagatagatg ttgatctcgt tcgtcaatac cgtcaccagg gaaatgttta gctgcaggca
    26821 aaattccact ctcttgaata cctttcatat aagctaaact aagttctaaa actttatcag
    26881 ggctttgtcc aaaagatcga ctagaaatga taggatttct ccaatttcta ttaatatcta
    26941 caataggagc aaaactccag ttacatccaa tagccgatgc ttcaattcca gaaactctcc
    27001 ctaattcata agcatatttc gcatcatttg ttgcaccaat ttttacttct acaccaactt
    27061 cggtaccatc tgtacaagct ccgttaccac ctgcttctgt attagcagca attaataaag
    27121 gaattttgct ttctgtttgt aggatatagt tttgatcata tacctcatca gcagttcctg
    27181 gattatatcg tacagctcca atatggtact gattgaccat actacttaag taatttttat
    27241 cacgactaga tcccatatta acaaataatt gacctatttt ttcttctaaa gtcatagcat
    27301 caatcgtctc ttcaatccaa gaaatagcat cttcatctaa atgatatggt ttttttcgta
    27361 agtctacttt tatagtcatt ttctttctcc tctacttatt agtaagattt ttccaaaaag
    27421 tatctatata tttttgttca atatctatat actctcccga taaacattta aataaaatat
    27481 cagttcttaa gttttcccat gattcttttt ctttattaac caaaatagga taaaagaaaa
    27541 ctttattctt atttgcagct tctagatcac caggagaatc accaatcatt aatatcttat
    27601 ccaaatcaca accattttca attacacttc gaataatgtc ttcttttttt cctttatctt
    27661 gacaacacag ttcatcaact aaggataata attcatgtct ttcccattct tcttgaatag
    27721 cttccttatt agctgagctt acaataatta tcttcccata ttttttcaga gcttttatag
    27781 tatctctagc acttaaaaag ggtttatcta atcctttagc ttgactaatc tgtcgattga
    27841 cttcttccga ccattttaaa gcttttaata aatcctctga ctgagagtat tctatttccc
    27901 tcttcaaaga tggattagac aattcttttg ttacagatac ccagtgcttt aatcgattaa
    27961 tcgatggtac atcatttttt tctaatgttt taactaaacc taaaaatctg tttattccac
    28021 gagtctctga atataaatta atattattcc aatcctgtag gaatttttcc ctatgatgaa
    28081 catcaaaaat ctctgcagca attggcccga aaaagagatt gtgtttataa gtcattgtat
    28141 ccatgacaca accatctgaa tcaatacaaa atatccaact gttcatgttt tttctcctag
    28201 ttatgttttt gatttagcaa attataattt tctaatactt tttctttaga taatggatat
    28261 acgaaaaata aaatcaatcc tacaacaaag aaacaaactg ctggaatcaa cgttgaagag
    28321 ttatacagct tttgtaaaac ttctccactt tgtacttttg ctaacgaatc ataccctatt
    28381 atagaaagag tccaacctcc gacaccgcca gctaaagctt gtccaatttt tcgggcaaaa
    28441 gaatatactg cataaatagt tccatcttct ctttggccag atttcacttc ttgatcatcg
    28501 attacatcag taatattcgc ccaaatgact gtattaaaaa tacctaaccc catatatcca
    28561 acagaagtaa gaactaagaa tacatacata ttactaggct ttatcaaaaa taaaagtaaa
    28621 tatacaatag cagagaagaa catccctact gacgccaatt cttttttacc gtatttttta
    28681 ctgaaaatta aacttacagg tgaaacaagt accaacatca aaataggatt gataaagttc
    28741 attaaaacta ttccctgtgc attattataa taatctggaa atacataatt gttcatactc
    28801 attgttaaaa gctgagcaag caacaaaaaa atagctgcag ctattataga aagcaacgct
    28861 ctatttttag ctaatccttt taaagatttc aacaatgaaa cttttaactg cgattcatca
    28921 cttttaaccc gttctacagt taatttataa catattacgt aacaaataac tgcacacact
    28981 gaaaatactc ctgccacaat tgtaaaagtt gatccaccac gtacaatttg agctccagaa
    29041 ctatctcttg tataaataaa aattggtgtt aacacgccaa tgaacatacc tgctaaggta
    29101 gctcctatac tacgaaatgt tgataacgct gtcctttctt ctgcaacaga agtgatagct
    29161 gatgccattg aaccgtaagg aatattaata gatgtataaa aaatacttcc ccatagtata
    29221 tatgttacat acatatatat aactttaata ctcatagaag ctgatgccat tgatgtttga
    29281 tacattaaaa aacttgctaa agcgactgga ccactcatcc gtaaaatcca cggtttaaat
    29341 ttgccagttt tagatggttt tgatccatcc acaatacgcc ccattgttat gtctgtaact
    29401 gcatcaataa atcttgctac taaaaataat gttcctacgg tagctccgct tatccccaat
    29461 acttttgtat aaaaaatcat taaaaaagaa ctagcaaaaa tgaaagtgaa atcattccca
    29521 aaatctccaa acatatatcc tattttgtct cttagaccaa attttctttc catataattt
    29581 gcgctccttc ctattgttaa gcgttttcat tctgttgaac tgccaactta cgtactatac
    29641 tctgaagtat aaagagttag tatttatttt ttacatttta tagtattatt tattttttct
    29701 cctaataatt ctttatctca taaaacttat ctatatattt actagctatt cgagttactt
    29761 cttcaaaatc acctttttca acaggagcca acaaattccc acctacacca actgcaacaa
    29821 cgcctgcttc aaaccactct tctaagttat ctagacttac tccgccagta ggcattatat
    29881 ttacatatgg taatggagct tgatacgttt ttacaacaga tggaccaaaa gcagaacctg
    29941 gaaatagttt aatgatttct actccagcgg taagtgcctc ctttatctca gtaattgtca
    30001 tacaacctgg taaataagga atttgatata aattacaaat ctctgctgtt tgtcgatcaa
    30061 aagatggact tacgatatat tctgctccag ccataattgc aatacgagca gtaaacgcat
    30121 ctaaaactgt accggctcca attattacat ctttttgatc agcatatttt ttagcaattt
    30181 ctttgattat ttcttcagca ttcggaactg taaaagttag ttcaatccct ttaattccac
    30241 ccgcagtaat tgcatttatt gctttaatgg cctcatcttt attttttcca cgtacaaccg
    30301 caatgactcc agccgcttct aacttactta aaatttctac ttttttcata tctatctcct
    30361 tcttttatgg atacatactc tagctagttg tactaactta caataaataa atttaagctt
    30421 ttattttttg tattattttc tactttcgat ttcaaaaaaa ttaaaataat tatacgcatt
    30481 acgataacaa atattctcca ctatctgact aagatacatt tcatcatctg gaattcgacc
    30541 tgctttaacc cactcgctta gtaattgaca taatactcga cggaaatact catggcgagg
    30601 atagcttaga aaacttcgag aatcagtcaa catacctgta aaattcgaca acaaactttg
    30661 ttgcgccatc gtagtcaact gttgacgcat tccttcagct gtatcattga accaccaagc
    30721 acatcccaat tggatttttt gaatcatacc cccttggaag ctttgcattg ctgttgctag
    30781 tggaagccaa tcggttggat ttaatgaata taaaattgtt tttggtaagc acccactctt
    30841 ttcagcagtg tcgaacaatg ctacaacatt ttgtgtcaaa ctagcttgat cccccatcga
    30901 atcaccaccg acatctactc caatttgttc ctttaatata cttgaattat ttcttaaagc
    30961 atttaaatgc atttgcatcg tccaaccata tcttgtatac tcagacatca aatgtaattg
    31021 aatacctgta acaaattgtg caatttctga taaatggaaa tccgtttttc ctgctaaagc
    31081 ttttattaac agattgtcga gtattttttc atctgctatt tcataaaaat aagtattcaa
    31141 accgtgatct gctaatcgac agccaatttg atgaaaataa tccactcgtt ggctcaatgc
    31201 ctgcttcaac ccattaaagt ttttgattgg tatacccgct gcttgactaa gttttaaaat
    31261 atagttcgaa aatcctgatg atttgatact ccagacctta tctggacgga aagtaggtaa
    31321 taccttgaaa gaattatcct gtttagaaag ctcagtatga tatcgcaagt catctgtagg
    31381 atcatccgtt gtacaaatgg ctttcacatc catcatcttc agcaattctt ttgggcgata
    31441 ggaatccgtt tggatttttt cgttggcacg tcgccaaatt tctggtgcat tcttctcaga
    31501 aattgtcaaa tcaatatcaa agtaacgttg taattccaaa tgagaccatt caaaaatagg
    31561 attacctggc gccttctcca ctgtttttac aaaagctaaa aatttttcat aatcattacc
    31621 tgcaccactg atcaactctt cttctatccc attagctcgc atcaatcgcc atttataatg
    31681 atctccagct cctttatcat ttaaccatac ctgagtcaaa ttcttgtatt gcttattttc
    31741 tagcaaacat gctgggtcta aatgacaatg ataatcgatg attggttggt gttctgcaaa
    31801 tttatgaaaa agtcttttag ctatttcatt ctctaataaa aagttcttat ctaaaaacat
    31861 atttaagcac tcctttcctt cccttagttt ttagaagaga aaatttttct tctaaataga
    31921 ctccattata taattattta ctcgccccct tttcaagcgc ttcccaaata ccatttaaat
    31981 aaactgcacc tagagctcgg tcatataacc cataaccagg tctaccggtt tctccccaaa
    32041 tcatccgacc atgatctgga cgaatatagc cattaaatcc attatcgtac attgttttta
    32101 atatcttata catatctaga gaaccatatt ctgatttatg cggcgcttca taaaaatcga
    32161 gtccattatc acttaaatat ttgatattac gaacatgtgc aaaaggaatc cgatcccgtt
    32221 gacaaaattc agatagaatt tcatagacat cattatctgg tcgttctgca atacttccag
    32281 tacataacgt aataccatta gaaggagaat ccactacatt acacaaccat tctaaatcgt
    32341 cacgattttt aacaatacga ggtaatccga aaatagaata tggtggatca tctggatgaa
    32401 ctgccatttt aatcccaact tcttcacatg tcgggataat tgctttgaga aaatatgcaa
    32461 agttctctcg caatttcgct tcatcaactt ctttataggc ttctaataat tgcttgattt
    32521 gtgataaccg atgaggttcc caacccggca attcaaaatt attagtagaa ttttctactt
    32581 ttctaataat ttcctctgga tcagtaggaa tattttttga aatataggct aacgtagaag
    32641 acccatcatc taaatgataa tccaaatctg attttaccca atcaaagact ggcataaaat
    32701 tataacagat aacctcaatc ccaaattctg ccaaattgcg aatcgtctgt ttataattct
    32761 cgatgtattg atctcgtgtt ggcaaaccaa ttttgatatc atcatgaaca ttaacactct
    32821 caataacttt caaggtaagg ccattggctt cgacttcatc ttttaaagct ttaattcgtt
    32881 ctctcggcca gacctctccg gctggtatat ccattaatgt tccaacaatt cctttcattg
    32941 ctgggatctg acgaatttct tgtagtgaga ttttttcttc atttgcccca taccatctaa
    33001 aagtcatttc catagttact ttcctccaac ttctgttata tatttattga ttgttttacg
    33061 aactgcttga ggacctacta gcatttcgca aaagattttt tcaattttct ttcctatctc
    33121 aacttgatat aaatctaaac caaatatttc cttattttct aagatttcct gtaccgcaca
    33181 atgaatcttt gcaggatctt gttcacctaa tttcaatact gagaattttt cctgcaactc
    33241 taccaataaa ggatcaggac ttggcacaaa attttttcct tgatcatcga ctgccaataa
    33301 ataacgtaac caagcagcaa tagcgatagg aataaatgtt agattttctg catttccgtc
    33361 tttttcgaca tactttttaa ttgtttcacc atagcgaatc gctacttttt gagaagtatc
    33421 tgttgcaatt cgttgcggtg tatctggaat atagggatta ggcaaacgtt tattgattac
    33481 ttcatcaata aattgttttg gatcaataac gcctggactt tcaactactg gtaagccttc
    33541 tacataacct atccctttaa ttaagccaac caaatcatca tcttgcattt ctgcctgaat
    33601 tgatttatac cccaacaaac aacctgttat tgctaatgcc gtatggagcg gatttaaaca
    33661 tgtcgtaacc ttcatgatat ctgctttatc aactgtttct ctatctgtca taatcacacc
    33721 agccgcttcc agtttaggtc gaccatttgg aaaactatct tcaatgacta gataatggac
    33781 tttttcagta ttggtaaaag cagcgatatt ggtatttttc gcagtatgaa ttatatctaa
    33841 gtcttcgaaa cccgccttca ataaatccat tttgacactt tccgaaggat ttggcgtaat
    33901 tctatcaatc atactccaag gaaaagtcac tttttcttta tcctttaaat aagcaataaa
    33961 tttttcagaa acgtatcctt ttttttgcca agcttctgca atcataaaaa tgctatcttg
    34021 aaaacgttga ccattctgtg agaaattatc agtagaaacc atcgcaatcg gtaactctcc
    34081 agcattaaac ctgctataca acaaggatgt cacaatactc atcgtatgtt gaggattggt
    34141 aggtccagat tttatatctg cttccactac cgaaaaaaat tgtcctttag aatcttttaa
    34201 actgtatcct ttttcagtaa tagtaaacgt tgtaaactgt aatgtaggat cttcaaaata
    34261 tttaaccact ctttgatagt ccgttatatt ctttggattg caatataaac tatcagctgt
    34321 tactgctaaa agtctttttt tgagttcacc atcttcaccc ataacaatct ccaaaatatc
    34381 attgttgtag tcatgataag ctttttctac tacttcttca tcaaatgtct cacacacaac
    34441 tactccggat tctaattcat tttgatctgc taagcattga gcaatttctg catgaaatcc
    34501 tcgataaaga tttcctccac caaaatgaag ccacactgga tgtttctgac cttcttgttt
    34561 gaccgcttcg acattaaaat acggtagttg aacttgcatt ttgttcaatc cttcgataga
    34621 atcttgccag tctttaatca aaaccatatt tacccctcca tactgatatg ttagtttccg
    34681 ttacaattgt agtataaaat cgaatgtaat cgctgtcaat aagaaaaaac taaaaatatt
    34741 cttttttcta ccatgtcaga attattgtgt ctttttttag taaaagcaca ccaatatata
    34801 ttttttagaa ctaacttaaa ttaaagctta atttctcttt gatcttattt gagtattttt
    34861 tgttttttta atagctagta tgttactttg aaatttgata ttatataacg agcatagaac
    34921 taaaatatca aattcaaaaa tgaaattttg agagggatta aatatgacaa ttaaaactag
    34981 tctccaaaat caagcttatc aatctattcg tcagcaaatt atctacgctg acttagaacc
    35041 tggaaaaaaa gtatcagaaa aaaaattgga agaaacgctt tctatcggtc gtactccaat
    35101 acgtgaagca ttgattcaac tccgtcatca aggacttgtt gatacaattc ctcaaagtgg
    35161 aaattatatc tcattaatcg acttagattt agcaaaaaat gctcgttttg tcagagaaca
    35221 tttagaacga caaattatgc tagagtgttg tgccaagatg aataatcaaa agaaacaagt
    35281 tttagaaact ataattgcag aacaagaaaa agctgttagt caaaagaatg ctcgtagttt
    35341 ttttcataat gataatttat ttcataaagc ttgttttgaa attgcagaac gggaagaagt
    35401 atggcaatgg ttggaagata ataatacgca cttggaacga tttcgttggt tacgagtaaa
    35461 aacggaagga ttaaaatggg aaaaaattat ggaacaacat catcaactat ttaaagcctt
    35521 gaccatgcaa gaccctgaag aagctaactt cttaacatcc ttgcatcttc atttaatgct
    35581 tgatgaacaa aatgttgtat tgactactca cccaaaatat tttaaaaata tataactgct
    35641 aaatttcttt tttttaattt atttacattt ctgattacta cagaaaacta actacttttt
    35701 ttatgtaaag ttctgtcaaa tatagtcact ttatattact ttctcttgag taaaaaaaca
    35761 tgtgaggtcg taaacaaaga ttgaactatc tacattcata ctctataatc tgtttatttg
    35821 cagactgata atagcaaaag aaaacttata atcaaataat atcaacattt gatcacataa
    35881 tttgagattg aatgctgatt tacctatgtt ttacgtaatg gtctttagtt atttttgctc
    35941 ataaaatgca taaaacacgc gtttttatgt gtgcttgaaa tttcttggct ctataatttc
    36001 taagactgat aaaaatattc tatcggttct ttttgcaaat aaaaaaacat atagaagctc
    36061 cattagattg aatcaccaca acacaatcaa aggagcattc cgtatgcttg attttcattc
    36121 tataaaacta cttttaggaa tcaaagaaaa tcacaacggt tcaaactaaa agcaagaata
    36181 tccgaagtac cattgtttca tcgatcatga gaaaagcatt cacctctgtc tgcagatgtt
    36241 cttatatcgc tctgtcagtc ctattaataa agtactgttt tgttttcaaa aaaactatta
    36301 cgagtacttt ttaagagaat gccttgcaaa tattcatcac acaccgttat ataagtcaaa
    36361 tagtttcttt cagtcgtcaa gaaaagattc tcctgtcctt ggcattatca gttaagtagc
    36421 aacttaattc tcggacttgt tctactctag caaaatttat tttcatcaac ttacgtccaa
    36481 atgttttttt gaataattac ttcatcgcgg gtctggaaga cttgcctcat tcgataagtc
    36541 actagctttt ggcgtccttc aggatttcta gtatacacca actgacattt gggcagcgac
    36601 gattgattta attcgtttag aaaaaggtta gcgatttgct tatttcggcc aaagcgttct
    36661 ggtaccgtca gaactatctt tttcccttcg attaaatacc gcggattctc cagcggtgct
    36721 aaaagctccc tcatagcttc agtaaaaagc tgtccttctc gatagccagc attctcaaga
    36781 tagcatctaa attcctgctg atcagccgct acgattactt ttctatcact aacttcacca
    36841 aggcgctgca acgtcgtttg aacgcatatg gccaagcttt ttttgcgggc acattccttt
    36901 tcccatagct tttgtcgcct acgaatttgg aaatattcca gcaagttacc tttaaatata
    36961 gctgaccaga agcttagagg ctctatattt tttactacta tcggcaatga agtttcttgt
    37021 ttttgcttta cctgatcgac tttcctaacg acgattggca tagataggtg ccagccatca
    37081 gctaaagcag tactccattg ttctttcaac cgacttcgtt cacaagaaaa atagaacatc
    37141 gactgagtat aagtttccat cgtggtggtc gtatattcca gtggtaaagc aaagcgatcg
    37201 ccaccactct caatttttgg tgaagattca aaggtcagac ctgaaatatt gcccaaacgc
    37261 cgtagcattc ttttcaaatc aggctgttca gacagaggaa ctcccggtaa tccaatcgct
    37321 aaatgccaaa tgttcgttac tttattttgt gcacctaggc gtaagccccg ccctcgtagt
    37381 tgttgtgttt gcacaaaagt ggaagcttga ttagccaaga tcaacagatt aattgcagga
    37441 caatcccagc cttctcctag caaactaacg gttcctacca aaatattcaa cttcttttcg
    37501 gttaataggc gagtaactgc ttgcatcaat gcttggcggt ttttgtcatc caccgtcaaa
    37561 taaaaataac ccgacatata aggatgttca ttcatacccg tcgctagttc tgccagctca
    37621 acagttacta gcattttcgg taacagtaag aactctccac agatggcacc gatttgctga
    37681 gtgttaccta atagacgaac aacttcatca aacaaaggaa ccacgccata agctaaggta
    37741 ccatcactgc ctgctaatac ctccttgcaa atacgatcca tcaaaatgac cgctgtcagt
    37801 tcttgcttca gctgaacttc ttcttttaac aaaatttgac aaactgcttg ctttttctca
    37861 ggaatcgcct gactaccaac agtgtctgga tactctggaa acaaatgcag cgactcatca
    37921 aacaatcatc cagctttttt caattgataa aaaataggca attccggttg atcttcaaac
    37981 atctgctgat aaatagtgat cagctctttt ttacctaaag atggaactga agctttttta
    38041 ccttgctgct tagaactgcc taatagctgc caatgttcgg gtatcaattc atagttatga
    38101 tgattcaagt aaattaatgc actagtgaac agctcaaaat tttcataaat ttccttcgaa
    38161 tgctcaagcg gatgctgaac aaattctaaa gaaagcaaat aatccaccgt ctcttggctt
    38221 tccattaacg cttccaccag ctcattttgt ttctctaaaa aggtaaaata atgctgagta
    38281 gtttcctgat tagccggaat caaatataga aaatcctgat gaggacatag aacctcctcc
    38341 tttaccaact cgggaatgct aatttcttcg tcaatcaggc cattttaact caatatattg
    38401 cttccactcc gtctgagagc tatcaatcgg aggcgtggct gtcaaggaaa tcgtcaacag
    38461 attcggttgt tttttcttca agtcaatcaa caattcaccc cagctttttt ttaaatgatg
    38521 agcttcatca aaaaactaac agctccagct gatcaccata agttggctcc tccttcagtc
    38581 ggttgtaaat ggtctgatac gtttccactg taatatgact aggatgttca atcgccccag
    38641 aaagatagca actatctgct ggatttacta agaaatattc ctcgattgtt gttaaccact
    38701 gtttcttcaa tattagggaa ggaaccaaaa ttaaggtttt acaatccaaa cgtcggatca
    38761 tctcaattcc taaaaaggtc ttgccagccc ctggtggagc cactagatgg actgaattgt
    38821 tatttaaata agtagcaaag ttagtcaata tacgcttctg atagctacgc cactgaattt
    38881 tttcatcaga tagccacatc ccaggtcccc ctctcacgta ttttctctat cataatctaa
    38941 atgaatacag caaaaaaaag atttcttcaa gaatatgtgt ttttatagta atattttact
    39001 tactctaaac tcaaatgaaa aggtaaaaaa actggtagta caatctttta aattctcatt
    39061 gttttgtacg ttctttacta tcaaaaaaac aaaaaaaacg cgtttctacg cgtgaccaaa
    39121 atttttagct ctataatttc taagactgat ag
//
LOCUS       DAJSBL010000022        38697 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.022, whole genome shotgun sequence.
ACCESSION   DAJSBL010000022 DAJSBL010000000
VERSION     DAJSBL010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 38697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38697
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.022"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(345..491)
                     /locus_tag="ONT85_001348"
     CDS             complement(345..491)
                     /locus_tag="ONT85_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EntF family bacteriocin induction factor"
                     /protein_id="HCR2378332.1"
                     /translation="MEEKNRLNAKQCSDQELKKIKGGAGTKPQGKPASNLVECVFSLF
                     KKCN"
     gene            complement(595..906)
                     /locus_tag="ONT85_001349"
     CDS             complement(595..906)
                     /locus_tag="ONT85_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HCR2378333.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQKVYLKLEKQKYE
                     LDPGPLINRLVNYLYFTAYTNKIRFTEYQEELIRNLSEIGRTAGINGLYRADYGDKSQ
                     F"
     gene            complement(908..1105)
                     /locus_tag="ONT85_001350"
     CDS             complement(908..1105)
                     /locus_tag="ONT85_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HCR2378334.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            complement(1499..3226)
                     /locus_tag="ONT85_001351"
     CDS             complement(1499..3226)
                     /locus_tag="ONT85_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HCR2378335.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(3219..4412)
                     /locus_tag="ONT85_001352"
     CDS             complement(3219..4412)
                     /locus_tag="ONT85_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HCR2378336.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(4599..5243)
                     /locus_tag="ONT85_001353"
     CDS             complement(4599..5243)
                     /locus_tag="ONT85_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR2378337.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(5337..5471)
                     /locus_tag="ONT85_001354"
     CDS             complement(5337..5471)
                     /locus_tag="ONT85_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HCR2378338.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(5473..7605)
                     /gene="feoB"
                     /locus_tag="ONT85_001355"
     CDS             complement(5473..7605)
                     /gene="feoB"
                     /locus_tag="ONT85_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HCR2378339.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(7602..8075)
                     /locus_tag="ONT85_001356"
     CDS             complement(7602..8075)
                     /locus_tag="ONT85_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HCR2378340.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            8476..8700
                     /gene="nrdH"
                     /locus_tag="ONT85_001357"
     CDS             8476..8700
                     /gene="nrdH"
                     /locus_tag="ONT85_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HCR2378341.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            8859..11018
                     /gene="nrdE"
                     /locus_tag="ONT85_001358"
     CDS             8859..11018
                     /gene="nrdE"
                     /locus_tag="ONT85_001358"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HCR2378342.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFLSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYAMAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            11068..12033
                     /gene="nrdF"
                     /locus_tag="ONT85_001359"
     CDS             11068..12033
                     /gene="nrdF"
                     /locus_tag="ONT85_001359"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HCR2378343.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(12122..13261)
                     /locus_tag="ONT85_001360"
     CDS             complement(12122..13261)
                     /locus_tag="ONT85_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCR2378344.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAV
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(13570..14283)
                     /locus_tag="ONT85_001361"
     CDS             complement(13570..14283)
                     /locus_tag="ONT85_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HCR2378345.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(14537..15238)
                     /gene="nagB"
                     /locus_tag="ONT85_001362"
     CDS             complement(14537..15238)
                     /gene="nagB"
                     /locus_tag="ONT85_001362"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HCR2378346.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            15472..17004
                     /locus_tag="ONT85_001363"
     CDS             15472..17004
                     /locus_tag="ONT85_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HCR2378347.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(17195..18397)
                     /locus_tag="ONT85_001364"
     CDS             complement(17195..18397)
                     /locus_tag="ONT85_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR2378348.1"
                     /translation="MKSNEKKPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMAALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTVFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQTVVFKG
                     KRNLENKK"
     gene            complement(18479..19168)
                     /locus_tag="ONT85_001365"
     CDS             complement(18479..19168)
                     /locus_tag="ONT85_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HCR2378349.1"
                     /translation="MKKKIIRLILISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(19274..19642)
                     /gene="rplL"
                     /locus_tag="ONT85_001366"
     CDS             complement(19274..19642)
                     /gene="rplL"
                     /locus_tag="ONT85_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HCR2378350.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(19695..20198)
                     /locus_tag="ONT85_001367"
     CDS             complement(19695..20198)
                     /locus_tag="ONT85_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HCR2378351.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     misc_feature    complement(20402..20529)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(20608..21297)
                     /gene="rplA"
                     /locus_tag="ONT85_001368"
     CDS             complement(20608..21297)
                     /gene="rplA"
                     /locus_tag="ONT85_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HCR2378352.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(21401..21823)
                     /gene="rplK"
                     /locus_tag="ONT85_001369"
     CDS             complement(21401..21823)
                     /gene="rplK"
                     /locus_tag="ONT85_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HCR2378353.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            complement(22210..22449)
                     /locus_tag="ONT85_001370"
     CDS             complement(22210..22449)
                     /locus_tag="ONT85_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004609551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR2378354.1"
                     /translation="MKKEYGYPTLKVICKASAGNETAIREILKFYDAYICKLCLRPFY
                     HSESGKITMQVDEELKGQIHTEMMKAILKFEIRVK"
     gene            complement(22454..22873)
                     /locus_tag="ONT85_001371"
     CDS             complement(22454..22873)
                     /locus_tag="ONT85_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008728841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HCR2378355.1"
                     /translation="MNTSSFEHIVRIQFNALMITVIKCTVKSRNRQFARRSKREVLFY
                     ELSDTKNLECVTEDKYSCDYISFEVLNFTIQISNEKLAVALYKLSSKERDVILLRYFQ
                     SMSDQEIAELYHVSRSAIYRRRSNGLKKLKTLLKERN"
     gene            complement(23114..23518)
                     /locus_tag="ONT85_001372"
     CDS             complement(23114..23518)
                     /locus_tag="ONT85_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HCR2378356.1"
                     /translation="MKKPKVAFVCVHNSCRSQIAEALGKYLSSNVFDSYSAGTETKPQ
                     INQDAVRLMKEMYQIDMEKTQYSKLLSEIPQVDIVITMGCNVNCPTIPCKYREDWGLN
                     DPTGKNDDEFLKTIHSIHAKILELTEKIKIMR"
     gene            complement(23548..23880)
                     /locus_tag="ONT85_001373"
     CDS             complement(23548..23880)
                     /locus_tag="ONT85_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008689081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HCR2378357.1"
                     /translation="MKLFGKKKETKSCCCGGNCTSETMQNAENKKQEQGIKILGSGCA
                     KCMELEKATRTAISELQLDYEIEHITDFAEIASYGVMSTPALVFNGEVISYGKVLTVD
                     EVKALLSK"
     gene            complement(23897..24892)
                     /locus_tag="ONT85_001374"
     CDS             complement(23897..24892)
                     /locus_tag="ONT85_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003062154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HCR2378358.1"
                     /translation="MWLFFQDQILGMKWLNGLIGNGLSLLGLDINSRLGGSIQFFLYD
                     VIKITILLCLLIFFISYIQSYFPPERSKKILGRFHGIGANIISALLGTVTPFCSCSSI
                     PLFIGFTSAGLPLGVTFSFLISSPMVDLGSLVLLMSIFGAKVAFAYVIVGLIIAVIGG
                     TLIEKMHMEKYVEDFVKNASNVDISSPTLTKKDRVQYAKEQVVGTFKKVFPYILIGVG
                     IGAVIHNWIPETWIESILGSNNPFGVILATLVGVPMYADIFGTIPVAEALLSKGAQLG
                     TILSFMMAVTTLSLPSLIMLKKAVKPKLLTLFIAICTFGIIIVGYLFNIFSTLFI"
     gene            complement(24988..25293)
                     /locus_tag="ONT85_001375"
     CDS             complement(24988..25293)
                     /locus_tag="ONT85_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR2378359.1"
                     /translation="MNDFSKDAKIFKALCDTKRLTILDYLKSGEKCACVLIENMNIGQ
                     SALSYHMKILCDSGIVNARQEGKWTHYSLSKSGSEYASKRLLELTTPNAENQSICCK"
     gene            complement(25521..26156)
                     /locus_tag="ONT85_001376"
     CDS             complement(25521..26156)
                     /locus_tag="ONT85_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR2378360.1"
                     /translation="MEGYAMKISHLGNNIQTIRKFRGMKQQELADKIGINMQSLSKIE
                     RGLNYPAYETLEKIMEVLDVTPNELLSGEWKYVNQSEKEVCQFLRTEERLNAELKHGH
                     YDNFFDSEEEWLEYELEKLREYITDYINGTNIEASDLYPIKEFIQHLKFQKLLDRYDD
                     LYSMDMFGESIEGHKYRTPYQVVKMINPNSKEDMELLREVLRNNHFDDEDE"
     gene            26666..27808
                     /locus_tag="ONT85_001377"
     CDS             26666..27808
                     /locus_tag="ONT85_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HCR2378361.1"
                     /translation="MNNLEFALEMKNKRLASGLSQRELAGLTHVSRYSINRFENGKAN
                     ASKETQNIILRCLNYYICDKPFYLLVDYLSVRLPTTDALEVIRKVLGMKADYFIHYDY
                     GYYGYKEHYAYGEIKVMASDDEHMGVFLELKGTGSRNMEYVLQAQNRDWYSFLNRCLD
                     CGGVIRRFDLAINDMCGLLDIPVLSEKYKNGGAECRCKNYENVQSGKLSGKNRNLAST
                     LYIGSKASTKYFCLYEKQKEQATKKKHTDIINRFEIRLRDKKAVQAVEELLLTYNPHG
                     LVFYLITDFVQFPDYPLWEIFISHDSLPFEMNPVPVNMERTLQWLERQVMPSIVMIEE
                     IDRLTGSNYMKMIDECTHLSEKQEMLVEQMCTDIADVIESEGVFYE"
     gene            27801..28007
                     /locus_tag="ONT85_001378"
     CDS             27801..28007
                     /locus_tag="ONT85_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HCR2378362.1"
                     /translation="MSNAQDIPVWEKYTLTIEEASKYFRIGENKLRRLAEENKDAGWL
                     IMNGNRIQIKRRQFEQVIDKLDAI"
     gene            28086..29279
                     /locus_tag="ONT85_001379"
     CDS             28086..29279
                     /locus_tag="ONT85_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR2378363.1"
                     /translation="MREKRRDNKGRILHTGESQRTDGKYLYKYVDAFGNTKYVYAWRL
                     TPTDPTPKGKRKKPSLRELEQQIRRDIEDGIDSAGKKMTLCQLYAKQNAQRANVKKST
                     QKQREQLMRLLKEDKLGARSIDTIKPSDAKEWALRMKDKGFSYNTINNHKRSLKASFY
                     IAIQDDCVRKNPFDFKLSEVLENDTKEKVALTEEQEQALLSFIKTDNVYHKYYDDMLI
                     LLKTGLRISELCGLTVADIDFKNEVVIIDHQLLKNKEQGYYIETPKTKSGIRQVPLSR
                     ETIQAFQRVMKKRPKAEPFVIDGRSNFLFVNHKGRPKVAIDYNALFVRMVKKYNKHHK
                     DNPLPHITPHTLRHTFCTRLASRNMNPKDLQYIMGHSNISITMNWYAHASIDTAKSEV
                     QRLIA"
     gene            29595..30263
                     /gene="sdaAB"
                     /locus_tag="ONT85_001380"
     CDS             29595..30263
                     /gene="sdaAB"
                     /locus_tag="ONT85_001380"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HCR2378364.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            30276..31148
                     /gene="sdaAA"
                     /locus_tag="ONT85_001381"
     CDS             30276..31148
                     /gene="sdaAA"
                     /locus_tag="ONT85_001381"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HCR2378365.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(31239..32258)
                     /locus_tag="ONT85_001382"
     CDS             complement(31239..32258)
                     /locus_tag="ONT85_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HCR2378366.1"
                     /translation="MEAGAFGYIRGGSEDEWTMKENTTSFNTKKIMPRILRGIDSADL
                     HTSVFGIELDTPIIQAPSAAQGLAHEKGEADTAKGVAAAGSIFSISTYANTTIKDAAD
                     AAPGAPQFFQLYMSKDDRFNEFILNKAVEAGAKAIILTADSTLGGYREEDVINQFQFP
                     LPMPNLAAYSEQSASGNGEGKGIAEIYAAAKQGLTPDDIKTIKEITHLPVIVKGIQSP
                     EDAVIAISAGADGIWVSNHGGRQLDGGPASFEVLPKIAEVVNKRVPVIFDSGVRRGEH
                     VFKALASGADLVAIGRPVIYGLNLGGAEGVTSVFEHLNKELSITMQLAGTKTIDEVKN
                     TKLMD"
     gene            complement(32431..33336)
                     /locus_tag="ONT85_001383"
     CDS             complement(32431..33336)
                     /locus_tag="ONT85_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HCR2378367.1"
                     /translation="MERKRCMTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEV
                     SDKEKTAESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDG
                     QIQATGDLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSM
                     GGVSALRYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQ
                     DYQQMIGNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTG
                     ENASHSMLHENQEVDQLVSRFIWKE"
     gene            complement(33395..33940)
                     /gene="nusG"
                     /locus_tag="ONT85_001384"
     CDS             complement(33395..33940)
                     /gene="nusG"
                     /locus_tag="ONT85_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HCR2378368.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(34041..34211)
                     /gene="secE"
                     /locus_tag="ONT85_001385"
     CDS             complement(34041..34211)
                     /gene="secE"
                     /locus_tag="ONT85_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HCR2378369.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(34235..34387)
                     /gene="rpmG"
                     /locus_tag="ONT85_001386"
     CDS             complement(34235..34387)
                     /gene="rpmG"
                     /locus_tag="ONT85_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HCR2378370.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            34549..35193
                     /locus_tag="ONT85_001387"
     CDS             34549..35193
                     /locus_tag="ONT85_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HCR2378371.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(35249..35752)
                     /locus_tag="ONT85_001388"
     CDS             complement(35249..35752)
                     /locus_tag="ONT85_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HCR2378372.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(35765..35811)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            36349..37314
                     /locus_tag="ONT85_001389"
     CDS             36349..37314
                     /locus_tag="ONT85_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HCR2378373.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGDDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(37413..38585)
                     /locus_tag="ONT85_001390"
     CDS             complement(37413..38585)
                     /locus_tag="ONT85_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR2378374.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMPTIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(38572..>38697)
                     /locus_tag="ONT85_001391"
     CDS             complement(38572..>38697)
                     /locus_tag="ONT85_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR2378375.1"
                     /translation="KLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
BASE COUNT        12024 a         7814 c         6759 g        12100 t
ORIGIN      
        1 tacgtcataa ccatggaagt aataatagat ctgaaatcat caataagcat acataatagt
       61 aatttattta aaagcactat attagttttc ttcttttcct ttaatataaa ataacttccg
      121 attataggta tagctgcgga aacatcagta aataattcaa caaaaaaagt gagtggtatc
      181 aataaacata caaaaagata ttttcttttg ttaagatcag tattagtcat ataaaataag
      241 agaaaaaaga aaaagtttcc cacgaacatt cttaatagat aagcttttgt atcgatagta
      301 atcaagagat cccctcctga ttttaggata tcataaaaaa aattttagtt acacttttta
      361 aataatgaaa aaacacattc cactaaattt gatgccggtt ttccttgagg ttttgttcca
      421 gcacctcctt taattttttt aagctcttga tctgaacatt gtttagcatt tagtctattt
      481 ttttcttcca ttacgattcc ccctacaaac tataaatact tttatgtcac tgattcaata
      541 tacctatttt tctaatcaaa aaattttgag acatatttaa tattataagt atcattaaaa
      601 ttgagattta tctccataat ctgctcgata taaaccattt attccagcag ttcttccaat
      661 ttcactcaaa tttcttatta attcctcttg atattcagtg aatcttattt tattagtata
      721 agcagtaaaa tatagataat tcaccaatct atttattaaa ggaccgggat ctaattcata
      781 tttttgtttt tctaatttta aatatacctt ttgtaaaacc tcaagaatat cagaatattt
      841 aggatcttta cttatagata tatcattata taattcatga acaatttgct tagcattttt
      901 tttcatttta gcacttccct ggaattgctc cacctaaaaa accacctata gacattcctg
      961 caatacaagt agttgccttg gcccaatcga ccgtacattt atttttagtg caatacactc
     1021 catttccata atattttcca ctatgagtgg tacccccata gataagttgt gtctctttaa
     1081 tagacaaaat ttttaaatgt ttcataatct aatcacccct aaattaatgt ttttagacta
     1141 ttattctaac attcattaaa tttcaaaaaa caattccttc ttgaaacgta gcaaaataat
     1201 tcctgaataa gaatttaatc acttttaagt atataaacta tgtatattct ttaatatttt
     1261 ttgagaatgc acttcgtaca taataaactt taatcacatt agtctttcat acctacgatc
     1321 gatctctttg actcgcaccc ataaattcat aatagtttgt cggcgtcttt ggacgtttcg
     1381 aagatgagct acgcattctc tttttttata gacaaaaaag atagagacga acatggagct
     1441 tccaaatttg gatatttgat ccaattttgg aagctccatt tatttcgtgt ctgtcttttt
     1501 attttttcca gctttcgact aattcttggt tctctttgat ccagtctttt gctgcttgct
     1561 gtggatcttt tccattgttg atttccaaca taactttttc catatctttt tctgaccaat
     1621 ggaaattgtc taatactttg taagcttctg gctggtcttc tttcaatcct tttcgaacca
     1681 tcgtatggat ctgctcttcg cttcccattc cgttttcagg atcttctaag tatttcagat
     1741 catatttggc aaacatccaa tgaggtgtcc atcctgtaac tacaattggt tcgtgttttt
     1801 tgatcgcttg acctagtgca acggtcattg ctcctgaaga agatgtctct actttccagt
     1861 ctttcaagtt gtcatatttt tggatcgtgt tttcagcagc cgtaactaca ccagctcctg
     1921 gctcgattcc tatgattttc ttacctgcct gatcggttaa ctcatcgatc gaatttacat
     1981 ccatgtaggc tggcactgct aaaccaactt ttgctcctgt caaattggca cctaggtctt
     2041 cgacttgatc tttatattgt gcatattgtg aaccatgagt tttaggtagc caagctgaaa
     2101 ccatggcatc tgactcgcca tttgaaacag atttccacat gattgaatta tctaatggcg
     2161 tcatcgttac atcatagccc atttgtttca atacttcacc gactacattt gttgaagcaa
     2221 cttctgtatc ccattcaaca taagataagt tgatacgttt tgtttctttg gcagatgaaa
     2281 aactccctat accaatcgca cctaagatca ctactactac agcaccaatg atcagtcctt
     2341 gacgtttttt acttttctgt gttgttgctc ctgcagcttt tttattgttt ggctggttca
     2401 aatgctgtgt aaaacggtca atgatgattg ctaagataac taatgcaacc ccgttgacaa
     2461 atccattccc gacttgtgct ctttgcaatg ctgataatac gccgcgtcct aagccgggtg
     2521 caccgatcat agaggcaata acgaccattg aaagtgacag cattgttgtt tgattgatcc
     2581 ctgccatgat cgtactttta gctaaaggca attctagttt gaatagtttt tgacgacttg
     2641 tgctcccgaa tgaatcagaa gcttctacta attctttagg aacttgtcga atccctaaat
     2701 tcgtaaaacg aacggttggt ggcagagcaa agatgactga ggcaaatacc cctggaacca
     2761 tcccgatacc gaaaaatgca actgctggaa tcaaataaac gaatcctggc attgtctgca
     2821 taaagtcaag gatcggtttg ataatacttt gggctttact gctttttgcc atcaagatcc
     2881 ctaatggtac cccaatgatg atcgagatca cactagataa aagaacaagt gtgacagtat
     2941 tcatcaaatc attccataga ccttggttat aaataaacca tagaccgatt aatgtgaaca
     3001 acgtaagtcc tggacgtttc ttagaaataa agaaagctgc taccgtcaat aacaagataa
     3061 acaaaggagc tggaatcact aacagtaaat ttgtaatccc cgacatcact gcctgtccaa
     3121 tcgtttgtaa aaatgaaaat aggccagaaa aagttgtcgt aaaccattct gtgatatttt
     3181 ctacccagtt tgccactggt aattgatagt tcaatagatt attcatgttc actcacctcg
     3241 cttgtttctg ctaatgcttc aatcacacta cctcggatga cgacaccttt taattttcca
     3301 tcctctacaa ctgcaagtgg agtcggtgag tcataaataa tattgaaaat atcacttacc
     3361 accatgtctt tcccaatcac cgtcacattt tcatctagga agtcgatcaa tggacgtttt
     3421 tgcttcctag cttctaacgc ttgatctgca gtgatgatcc ctttcaggtg tctctttctg
     3481 tcaacagcca tcagcatgct gacttcttca ttacgcatcc ttgtcaatgc aacgtttggt
     3541 ccatcagatt cgatattcgt cgtcaatgca ggtaccatga tgttttgagc cgtaagcact
     3601 ttggaacgat cgacttcttc cacaaattca cgtacataat catttgctgg attggtcaat
     3661 atttcttcac ctgttccgat ctgcatgatt tcgccgtctt tcatcaaggc gattctgtcc
     3721 ccaatccgta aagcttcatt caaatcatgg gtaatgaaaa taatcgtttt ttgaacattg
     3781 gcttgcaggt ccagtaattc gtcttgcatt tctcgccgga tcagcggatc aagagccgag
     3841 aacgcttcgt ccatcagcaa gatctcagga tcatttgcta aagctctggc taggccaacc
     3901 cgttgttgca tcccaccaga taactgacta ggatattggt ctttaaatga aagcaaacta
     3961 gaattttcta atgctttttc tgctttttct tgtcgttctt ctttaggtac accacggact
     4021 tctaacccgt attctgtatt ttccaagatc gttcgatgtg gaaacagacc gaagttttgg
     4081 aaaaccatat tgattttatg acgacggact tctcgcaatt cttctttgct taacttcgat
     4141 acatcttctc catcaatata gatactgccc gaagttggct caatcagacg attcaataga
     4201 cggatcaatg tggatttccc actcccggaa agccccatga tcacgaagat ttctccttct
     4261 tttacgtcaa aattcacgtc atacacacca accgttgcac cggttttctt taaaatctcc
     4321 gttttcggct ggtgatcatt catcatatct aaagcggctt gtgtcttttt accaaatatc
     4381 tttgttaaat gttttacttc tacttttacc aaacaaattc tcctttttta tgtaaattca
     4441 tttttctttc tgaaaaaagt gaccacacta tgttaacatc atgtagtcac tttgtcaaaa
     4501 atgaaagaaa tttgcttgtc caaactgaaa aaaacttggc taaatctttg taaataaaag
     4561 gtttagccaa gttaatattt ttttaatatt tcacacaatc attctgcaag ctgatgctcc
     4621 gggtaaaaaa agtttgtcat acgctgtagt gtatattcat ttccaataaa atgaacgatg
     4681 tcccctgccg taaatttagc ataggggcca ggtgaaacaa gcagttcttc attatgagaa
     4741 attgcgataa ctgtggcgcc agtcgcctgc cagatattca actcgctaat tgaacggtcc
     4801 agatgcattg ctttttctga taaagataat tcaaacggaa gtaaaggatt catcttatgt
     4861 attcgcttcg tttgattgac cagctgatcc aataattgag aaaaatgatt cagctcatct
     4921 tgttgcttct ccacacttgc catgatttct gcttttgtat cttggatgga ttgtacatct
     4981 tgatattgca ataggaaatc ttttgctttt tccttcgaag caacaaatgc cccacttcca
     5041 tgccgtactt ccatgattcc aagatctacc agcacattga tagccttgcg ggtagtctca
     5101 ggagaagcat tgtacgtact ggcaagtgtt gagcgggcat ggatcttctc acccacttta
     5161 tatcggtttt caacgatacg ttcggctaaa tcaacagcaa tctgctggta gcgcgggcgg
     5221 acgatttttt tctttccaga cattttcttt cctctttctt tttttacttt ccctcatctt
     5281 actatgctct agcaaaataa gcaaaaaagg ggagaatagc ctcttgcaac gactgatcat
     5341 cgcttgatct gttccttttt tactgggcaa gctgtttgac agtcatcaca attcttaccg
     5401 gattttaaac gagtatagac aattgcgcct gcacttccaa agattaacac acttaataag
     5461 aatgtcgcca tcctattcct ctccttctaa tgacaatata gtataaaact ggttcttgga
     5521 ttttggctga cggaacaaaa cataaacgag tcctatcatc agtaaaatag caagatacgt
     5581 ccctaacgct acctgtccac cctccaataa gtgtcccaac tgataaatca caaaactaac
     5641 taaataagct aaaccgcatt gatagcctat ggcgatccaa gtccatttct tggcattcat
     5701 ttcccgatgg atagccccaa ttgccgcaaa acaaggcgca caaagtaaat tgaaggctaa
     5761 caatgaataa gctgctgcgg gagtatatgt gtgttgtagc acttgccaga tttctctgcc
     5821 attttcggat acttccgcat gaccaaacaa aataccaaat gtgccaataa tattttcttt
     5881 agcgaccaat cctgtaatcg ctgcgactgt tccttgccag tttccccaac caagcggctg
     5941 aaaaacaact gccaggattc gcccgaaaaa ggctaagata ctttggtctt cagaaacagg
     6001 ttgagcataa aaattataag aagagctgaa ccagatcacg atactagaaa caaagataat
     6061 cgttcctgct tttttaacaa aagacttgct gcggtcatag gtttgtctta ggaccgctga
     6121 ccattgagga aaatgatatg cgggcaattc catgataaaa ggagctgtat ctccagcaaa
     6181 caaacgtgtt tttttcaagc tgattccaga gaaaataatt gcagtcatac caagaaaata
     6241 ggcagaagga gctacccaag attgatgagg aaaaaaggcc cctgctacta aggcaataat
     6301 cggcaacttt gcagaacaag gcatgaaggt tgtgaccatg atcgtcaaac gacgatcttg
     6361 ctcgttttca atcgtgcgac tggccatgac gcctggcact ccacatcctg tagcaatcag
     6421 cataggaatg aaggattttc ctgaaagacc gaatttcctg aaaagtcgat ccatgacaaa
     6481 agcgatcctc gccatatatc cgcagtcttc cagaaagctc aagcataaaa acaatataat
     6541 caattgcggt agaaatccta acactgctcc cacgcctgcg atgatgccgt tcaagatcag
     6601 ctcctgcatc catcccgcta cttgccattc agctaacaat ctgccaacat gttctggtaa
     6661 ccatgtacca aacaatgtgt cattgatcca atctgtcccc attgttccta ctgtttgaat
     6721 agccagataa tagatcagcc acatcacaaa tgcgaagatc ggcaaggcta accaacgatt
     6781 cgttgcaatc tgatcgattt tgtcactcat cgataattgg aaagaatcat ttgacacaac
     6841 acagagtgta catagttggg taataaattc ataacgttca ttgaccagaa tcgtctcact
     6901 atcgtcgtcc aatagtttct ctgttaactg gatcagttgc tcgatctctt tttgctgaga
     6961 aactgctatc tcgtaatcct ctcttacctt ctgatctcgt tcaaaatatt tcattgcata
     7021 ccagcgtttc ttgccgcttt gttctcctag tacatcttca atttcatgta aagctgcttc
     7081 taaacgtgga tcatatgtac agggaacagg aatagaaggc cgcttttttg cagactcctg
     7141 aatcttttcg attagttttt cgattccttg ctttttcaat acgctgattg gtaaaactgg
     7201 tacaccaaga ccataggaaa gcttttctag attgattttt tttccttctt ttttgatgac
     7261 atccatcata ttcagaccaa tgatcatagg aatgtctaaa tccaataact gcgtagttaa
     7321 ataaagattt cgttctaaat tggtagcatc aataatgttg atgatcagtt caggctgttc
     7381 ctgaaccaaa taatctcttg caatcacttc ttctggtgta taaggagaca aagaatagat
     7441 gccgggtagg tcctgaatat gaatgttcgg attttttttc aacgatcctt cttttttttc
     7501 tactgttacg cccggccaat tccctacata ttgattcgaa ccagtcaaga gattgaacaa
     7561 gctagttttc ccgctgtttg gatttccgac taacgcgata ttcattcttt ttcctccctt
     7621 tgtatcaaga ttagttcggc ctctgacttt cgtaacgtca gttcataccc tcgcactgtc
     7681 agttcgattg gatctcccag aggggctagt tttcggataa ctatttttac atttcgcgtc
     7741 aatcccatat ccattaaccg tcgcttcact gcgccttgtc cgtggattgc tacgacgatc
     7801 ccagtctctc ctgcttttaa ttgatcaaga gaagtccagc tttcagagga agcagtcttt
     7861 ttattgacaa agatttgacc tagtatttcc cgacttactg ccactcgatg actttttacc
     7921 agtaaaatgg cattgttgcc tgataaattg acgacgacga ttttacttcc ggcaacgatc
     7981 ccgatatttt gcaaatggcg ttttgttttt tcttctgttt cgattcgttc gacttgataa
     8041 acgaaattct gctttgcctg cgctaaggtc tgcatattga ttcctctctt gtgaaataaa
     8101 tcatgtatta aaatcattca ttatatgttc taattttctt acgtgttacg caaataaatg
     8161 agaatcattc tcacatagaa tactctcggt aattgagaat gtcaattata cttgtgatat
     8221 tatgagcgaa tctttagtat tgatagataa aaatgagaaa aaaatatttc ttcatagttt
     8281 tccacaattt tttctctatt aaatttgcta ttttcccaca cgatacttgt tttcagctct
     8341 tttttcaaaa acacaaaact tagtattcca cagtttccac aacacaatat gtagtgtttg
     8401 ttgtttgact tttttactaa gagttttata ctatctatta tagataataa gaatgtgtta
     8461 gaggaggcac tcaccatgaa cgtaaaaatc ttttctaaaa ataattgtat gcaatgtaag
     8521 atggtcaaac ggtttttaac agaaaatcaa attgcttttg aagaagtcaa tatcgatgaa
     8581 cagcctgaag cagtagactg gttgaaagca caaggattcc aaagtgttcc tgtcattact
     8641 tctgatgcag aaacagttat cggattccgt cctgatcagt tgcgtaaact tgccagctaa
     8701 catgactaaa agaatcaaaa cgtgatacat ggatttgaag ggcttgagac agcaccagtt
     8761 tttgtcgcaa acttccaaat ccttttttaa agcttaaaaa caatgcaatg atttgcaatc
     8821 aagcatcatc aaatacgaaa tgaaggttgg gcgtacgtat gagtcttaaa aatttacaag
     8881 atgtcagtta tttcaagctg aataatgaaa tcaatcggcc agtcaatggt caaatccctt
     8941 tgaataagga taaagaagct ttagttgcct tttttgagga aaatgtcaaa ccaaatacga
     9001 tgacttttct atctattatg gaaaaaatca attacttgat cgatcaagaa tatatagaag
     9061 cagaattcat tcaattgtat tcaccatcat ttattgaaaa gctatatcaa tttttggacg
     9121 agcaacattt ttctttcaaa tcttttatgg ctgcttacaa attttattct caatatgcgt
     9181 tgaaaaccaa cgacggttcc gcttatctgg aaacttatga agatcgagta gccttcaatg
     9241 cgctttactt tgcgaacggg gatgaagaat tagcacttgc tcttgcagac gaaatgatcc
     9301 atcaacgata tcagccagct acaccttctt tcttgaatgc tggacgtaaa cgtcggggcg
     9361 aattggtgtc ttgtttcttg actcaagtca ctgacgatat gaactctatc gggcgctcaa
     9421 tcaactctgc tttgcaattg tcccgtattg gtggcggcgt agggattact ttatcaaatc
     9481 ttcgtgaagc cggcgctcct atcaaaggat atgagggtgc tgcaagtggt gtcgttccag
     9541 tcatgaaatt attcgaagat agtttcagct attctaatca attaggtcag cgtcaaggag
     9601 ctggcgtggt ttatttgaac gttttccatc cagacattat tgctttcctt tcagcaaaaa
     9661 aagaaaatgc agatgaaaag attcgcgtaa aaactctttc tcttggcgtg atcgtacctg
     9721 ataaatttta tgaattgaca cgtaaaaacg aagatatgta tttatttagt ccatatagtg
     9781 tcgaaaaaga atatggtgtc ccattctctt atgtaaatat tacagctgag tatgatcatc
     9841 tagttgcaaa tccaaacatt cgcaaacaaa aaatcaaagc gcgtgatctt gaaaatgaaa
     9901 tctcaaaatt acaacaagaa tctggctatc cttacgtaat caatattgat acagcgaacc
     9961 gcgagaatcc aatcgatggc aagatcatca tgagtaattt atgttcagag attttacaag
    10021 ttcaaacgcc ttctgtgatt aacggcaaac aggaatatga agtattagga acggatatct
    10081 catgtaactt aggctcgaca aatatcgtga atttgatgca aagccctaac tttggtcaat
    10141 ctgtgcgtac gatgactcgt gcattgacat ttattacaga tgcatctgat atcgatgtcg
    10201 taccaccgat ccaaaacgga aataagctga atcatacgat tggattaggt gccatgggct
    10261 tacacactta tcttgcaaaa gaacaaatgg aatatggctc agaggattca ttagacttta
    10321 caaatattta cttcatgctg ttgaattatt ggacacttgt tgaaagcaac aatatcgctc
    10381 gtgaaagaaa acaagttttc cataattttg aaaaatcagc ttatgctgac ggcacttatt
    10441 tcgaaaaata cgtaactgga gaattccagc caaaatcaga aaaagtcaaa gcattgtttg
    10501 aaggcatttt tatcccaact gctgaagatt ggaatgcttt gaaacaagca gtaatgaaag
    10561 atggactgta tcatcaaaat cgcttggcag tagcaccaaa tggctcaatt tcatatatca
    10621 acgatacgag tgcaagtatt catcctatca ctcgcatgat cgaagaacgc caagagaaga
    10681 aaatcggtaa aatttattac ccagcgcctt atctatccaa tgagacgatt ccgtattaca
    10741 cttctgctta tgatatggac atgcgtaaag taatcgatgt ttatgcaatg gcacaacaac
    10801 acgtcgatca aggcatgagt ttgactttat tcatgcgttc tgaaatccca gaaggcattt
    10861 atgaatggaa gacaacaacg aagcaaacga cacgtgattt gaatatttta agacattacg
    10921 ctttccacaa aggaatcaaa tccatttatt acgtacgtac ctttactgat gatgcagaag
    10981 aaatcggaag caatcaatgt gaaagctgcg tgatctaata tcatagatag attcgcttat
    11041 tcatatttta attaaaggag cattctcatg tcagaaacat attatgaagc aataaactgg
    11101 aatgaaattg aagatatcat tgataaatcc acttgggaga agctgacaga gcaattttgg
    11161 ctagatacac gtatcccact ttcaaatgac ctagatgatt ggcgtacatt gtctcaattg
    11221 gaaaaagata cagtaggaca tgtatttggc ggtttgacac tgctagatac tgtccaatct
    11281 gaaagtggca tggatcagtt gcgtaaagat gtccgcactc cccatgaaga agccgttttg
    11341 aacaatattc aattcatgga atccgttcat gccaaaagtt attcttctat ttttagtaca
    11401 ctgaatacga aaaaagaaat cgaagaaatt tttgactgga caaatacgaa tccttatctg
    11461 caaaaaaaag ctgagcgtat caatgaaatc tacaaacacg gtacaccgct agaaaagaaa
    11521 attgccagcg tctttttaga aacattcttg ttctattcag gtttttatac tcctctttat
    11581 tatttaggaa acaacaaatt ggctaacgta gcagagatca tcaaactaat catccgagat
    11641 gaatctgtac atggaaccta catcggttac aaattccaat taggattcaa cgagttacct
    11701 gaagcagaac aagaaacgtt aaaagactgg atgtacaatc ttctttatga gctttacgaa
    11761 aacgaagaac gttatacaga agaattatac gatccaatcg gctggacaga agaagtcaag
    11821 acattcctac gctataatgc caacaaagca ttgatgaacc tagggatgga tccactcttt
    11881 ccagatactg ccaatgatgt caacccaatc gtaatgaacg ggatttcaac aggcacaagc
    11941 aaccatgact tcttctcaca agtcggaaac ggctatttgt taggtcatgt agaagcgatg
    12001 aaagacgacg attatttgat tggattggaa taaggattta ttttaattca aaaatttttc
    12061 gaaaaataaa aagagtttag cgtacagata ttttaaatgt acactgaact cttttttatg
    12121 cttattcact tgtttcttcc agcacttttg gactgtctaa cttttccgaa acatgttcca
    12181 ttcttctcaa taaatagatt ttccggatat ttaatattat cgtcttaatc actgcataga
    12241 caggtatgca aatcagcatg cctaatattc ctgcgacact tcctgctcca atcaatacca
    12301 agatgatcgt caaaggatgg atattcattg atttcccaaa taacaacggc ttgatcaaat
    12361 ttccatctaa ttgttggatc actaaaatcg acaacgccat atacaaagct tgaaatggag
    12421 agataaacaa accgacaatg attgctggta ccgctccaat aaaagggccg acatacggga
    12481 taatattcgt gattccgcaa atgaccgcaa gcaacaagct ataaggctga tcgaatattc
    12541 tcattgccaa aaagctcatt acaccaataa tcaaagcatc taagatcgta ctgctgatat
    12601 aggcagataa tgtttcgttt aattctttta ccgtttggcg taactcttta cgaatcgctt
    12661 tcggaaagaa gtgagacaac gcatcaataa atttgtgccc atccttgaac ataaatagca
    12721 aaataaatgg aacggtaaat aacaaaataa aaaattgcat cataaatgaa atgatttttg
    12781 agacacttgc agataaactg actaatatga tattgaacaa attactaaga gataaatttg
    12841 ctgatgctaa ttgttcttcc aaattgaaat ttttaaactc ttcccgctgc gccaattcat
    12901 ttaaccattt gcttgtttca tctgcataca ttggcaagct ctgggtcaat tgaattgtct
    12961 gttccattaa ctgaggcact acattcatga cagttaaaac aactaaaatg attacgacca
    13021 aaaacgtcaa taaaaaaccg aacactctcg ggatccgatg cttttcgaga aaaacaacga
    13081 ttggatcgaa catataatag ataaaaccag caacaagcaa tggtaaaaaa actgccgaaa
    13141 gcattttcaa tattggacta aaaatattcg gcatttgtaa aataaagtac acgccgatga
    13201 tcgtgagtac tagctccact gtccagaaaa ataattttga ttgtttaaaa cgtgattgca
    13261 tacatctcct ccaaggtcct ttgatacttc tattctatca tttaacaaag agttacgcgc
    13321 tgagattttc atttgcttaa aaaaaacaaa aaaaataaac atattttaat gagtagacga
    13381 gtacttaaac aaaaacacgg tttggagtag tgacaaaaat tatactgcag tccctttttc
    13441 caaataaacg gtgttcaaaa gcttgcatct tcgtaataag cccagccaaa cgaaaaatat
    13501 aaggaactat ttttatttgg ctgggcttat tacttgatgt aggacatcta ttttacaacc
    13561 tcagatcatt tattgcgcaa tactgactaa atgcaacttt gtgatatttt tgtgtacgga
    13621 caactcctcg atcacatcgg cataagtgag cccttttgga agatcaatcg tatagatatt
    13681 tttgtacact cggtagtcat caacgaattt tacgtcaaaa ttcacatctt cgattacgat
    13741 atttttctct tcaaaataat cggtaagaaa atctttggtt tcttttctat ggataaattg
    13801 gatttctaac tttttagtcg taggtacatg gatcacgcgt ttcactactg tgagtgcaat
    13861 agcaatcccg ataaaactga tgatcgcaat ggaatagtaa cccatgccga tggcgatccc
    13921 taatccagca attgcccata aagaagcggc agtcgtaagt cccgtcaccg attgtttcgt
    13981 gacgatgatc gttcccgcgc ctaagaaacc gacaccactc accacttgag caatcagcct
    14041 tgcttcatct gaacgaatcg ttccagcaag ctgtggatac tctaaagcat ctcttaatgc
    14101 accactagcg atctcaactt ggatcaatgc aatgatacat gctcccatac aaactaaaat
    14161 atgcgtacgc atacctgctg gacgattttt atattgtcgt tcaaaaccga ttgctccgcc
    14221 aaatagcata gcgatcccta aacgtaaaat aatctctgga attgtcaata ccatatcatt
    14281 catctaaaca ccctccagtc caatatatca tgtctgaaag gagcagacaa aaaaagtgca
    14341 atttttacct cttacgaaaa aaggactgtg acaaaatttt tgtcatagtc ctttttatga
    14401 ataaacggtg ttcaatcagc tgaccctcgg tattactcaa caaacggcaa aatatgggga
    14461 gctatttttc ctttgtcttc tctaatactc aggtcaaaac gctgatttca caacctcttt
    14521 agcctactat tgaaaattac aacttacttg ctgcttcttc atcgatgatc acaacaacat
    14581 tgtcgtgatt ttgcaataca cttgctggca aatgattcgt tacaggtcct tcaatcatcc
    14641 ctttgattgc atcagctttg ctttcgccaa atgccatcaa gacgatttct ttacttttca
    14701 taattgaacc gattcccatt gaaacagctt ttgttggtac atcttctgct ttatcgaaga
    14761 atcgtttgtt tgcattgatt gttgattctg ttaagtcaac tactgaagtc gttccgtcaa
    14821 atgaagcacc aggttcattg aaaccgatat gtccattttg cccgattcct aagatttgga
    14881 tatcgattgg atgtgcatcg atgatttgat catacttttt gcatgctgct tccagatctt
    14941 ctgcttttcc atctggtaca aacgtttctt tgaatggttt tttatcaaat aattggacat
    15001 ccatgaaatg acggtagctt tgttcatctg aaccgcctag tccaacgtac tcatctaagt
    15061 ttacagaagt catttcactg aaatccaaat cgcttgaagt catttcttta tataaagtaa
    15121 ctggtgtgct tcctgttgcc aatcctaata cttttgcacc tttgttcata ccttctttga
    15181 tgatttcaaa tgcttttttt ccgccttctt cagcgttttt tacacgaatg atttccatag
    15241 tgattgctcc tttatatttt ggtatagccc aattatagca ttcttttttt ctttttacaa
    15301 taattagtat agaccaaatt gcattttttt cattttcttg acaataaata ggaatccgtg
    15361 ttaggtttca taagatggtt caatcaaaca atccatgttg catgaaaacc aagcaagtga
    15421 ttcactgtgg acgctttatg tctgcagttt ttttatgaaa aaggagttat tatgagtata
    15481 ctgacattaa acaacattac ccagcaattt ggcgacaaaa tcctctatga aggtgtcgat
    15541 ctgcaattaa atgctggcga acatcttggg ctgattggac aaaacggagc cggtaaaagt
    15601 acattgatta aaatcatcac tggtgagatc ttaccagatg acggacagat ccattggcaa
    15661 aaaaatattc acaaaggtta cctcgatcaa tatgtggaag tcgatgaaac attaacaatc
    15721 gaagaatttc taaaaactgc gtacgcaaaa gaatacgaaa aggaagaaaa aattgctttt
    15781 ctttaccaaa catatagcga gaccttcgat gattccttgc tggaaaaagc tgggaaactc
    15841 caaacggaat tagaccaagg agtgttttat caaatcgaca ccttagttgc tgagatgagc
    15901 agcgggttgg ggatcgatgt cttaggttta gattcactgt tgaaaaatct aagtggtgga
    15961 caacgttcaa aggtgatttt agcaaaacta cttttagaaa acccagatgt tctgatctta
    16021 gatgaaccaa ccaatcactt agatgatcag catatccaat ggctgacgca gtttcttcag
    16081 gattttgacg gtgcgtatat cgtgatttcc catgaccaag aatttcttga tcagatcacc
    16141 acccatatcg cagacatcga gtttggaaag attactaagt atactggcca tctaaaacaa
    16201 gcattgaaac aaaaggaaca aaatcgcgaa agctatttgc gccaatatca tgctcaacaa
    16261 aaacaaatcg aaaagaccga agcttatatt cgaaaataca aagccggttc ccgatcaacg
    16321 atggcaaaaa gccgacaaaa acaattagat aaaatcgaac ggctgacgcc tccagcttca
    16381 gcaagtaaag ctgtctttga ttttccattc tcgcctattg tcacgacatt agcagtggaa
    16441 acaactgatt tagtcatcgg atatgatcga ccgctacttg cacccatcaa tttgaccatc
    16501 cgctttggtg aaaatattgc cattcgaggc ttcaacggga ttggaaaatc tactttatta
    16561 aaaacactga tcggtgagat tgaaaaaata agtggcgatt tccacttccc agataacaca
    16621 aaaatcaatt acttttcaca agatctgtac tgggaaaatc cattagaaac accactacaa
    16681 tatttaagca gtcaatttcc aaaagcgact atcaaagaat tacgtcgcca actagctaaa
    16741 gcagggttag ttaatcaatt agcaagtgaa cctctcacta cattaagtgg tggggaacaa
    16801 actaaggtga aacttgcaca gttgacaatg aaccaaggta atttactgat tctcgatgaa
    16861 ccgacgaacc atatcgatca agaaacgaaa gaaagcttac aagacgggat acagaaattt
    16921 ccaggaacag taatcatcgt ctctcatgaa caagaatttt atcaagatct tgtgcaacga
    16981 gtcattgaga ttgaaccaaa ataaacgttt gattgaatag ttgaaaaaaa ggagctgtga
    17041 caaaagtttt gtcacaactc cttcttctga ataaatggtg gccaattagc tgaccttcgg
    17101 tattagctca aaaaacggca aaatatgagg agctattttt atttggctat tcttattact
    17161 tgatgcagaa cagcttttca caacctcttt tcgtctattt tttattttcg agatttctct
    17221 ttcctttaaa aacaactgtt tgaaagaaga acgcacatcc ttgaaaaaca agcccgctaa
    17281 ccgttacacc taaccagcca tactgctgcc acattgtcgt ccctagcaat gaaccagccg
    17341 cacctccaat aaagtacata aacataaata cagtattatt tcggttgctt gctgcttcac
    17401 ctagcccttg tactcgtgtt tgattggcga cttgaccaaa ttgattcccg acgtctaata
    17461 aaataatagc taaaagcaaa atggccaaat ggcttccgcc aaccaataaa gcaataaagc
    17521 ttattgtttg cataacaagt cccatataca cgatttttct ttcagaataa gaatctgcca
    17581 gtcgtcccac gataggtgca gcaactgctc cagacaaacc aaaaatcgct aatatccctg
    17641 cttcgaatgt tccccaattg taggcgggac tgctgatata aaaaatcaac gtagaccaaa
    17701 agatcgaaaa tgttccaaac atgaaaaatc cactgactgc cgcttctctt aatagacgtt
    17761 gagaagcaac taaatgcggc aaacttttaa gtgaatcaag gtatgaaaca gctgtactac
    17821 tttgctggac agaacgcggc attttccagt aaagcagaag tgtaagaatt ccgactgcac
    17881 ataaagcaaa taaatagatc atccgccaag aaaaaagact ggcaacaata ccagataccg
    17941 tccgagaaag caaaatcccc acgagtaaac cgctaagcaa tgtacccatg accttccctc
    18001 ttgcttttgg tcccgctagt accgcaccgt aaggaatgat gatttgcggc acgatcgaaa
    18061 gtaatcctat taaaaaagaa gcgcatgcaa ataacgtaaa cgaaggcgca aaaaaagcgg
    18121 ccaacagaga gacggcagat aacgctgcca ttcgaatgat caattttgga cgatccacca
    18181 cgtcccctaa aggaactaaa aacagtaacc ctaacgcata gcctaattgg gtcaacatcg
    18241 tcaatatccc gactgcactt gtgctaacag ataaactagc tgctactttt gttccaatcg
    18301 gctggatgta atacatattg gcaacaacaa cgccacaagt gattgataat agtaaaataa
    18361 gcgaagttgt taaaggtttt ttttcattac tcttcattga tttcccccat ttcctatctt
    18421 agtttacaac ttatggaaaa gattggaaat agaaaaatac aactattcgc cacacttatt
    18481 atttgtgctt ttgttttcct tgttttttct tgcgatcttt ataaatgaag acaactaacc
    18541 ctactagcac agtaacactt gcaaccaacg tgactgcagt tttgttatat ccgtgaaaca
    18601 gagcttctcc accacacgca tacaacacag acgaagggat gactccaatc acgatcgaca
    18661 atatgatttg ctgtagaggc aatttcatcg catctgcagc aaaattgacc atagaagatg
    18721 gaataaccgg tatcatatag cctaacatta cacctatttt tggatgtttc atttgtcgga
    18781 ttgcttgcac ccaatgattc gtttcggttt tcttatctaa aaattttaag cgatgcataa
    18841 ggaaaattgc agtgatgtta ccaagagcat tacccaaaat gttgatgatc gcacctgtca
    18901 gtggtccaaa gctcaaacca gcaatgactc caactactga agtaggaata cctggaacag
    18961 cacacatcac cgctgtcaat acgatcaaga gtatcgatgc aaaagtccca tgcgaacgaa
    19021 tatgtgcgag tagctctgcc ttactcgctc tcggatttaa aaataattgg atgtctgtct
    19081 gatattctaa atacaaccga tagcccacta ccagtatcag aatgattccc aacgagatca
    19141 atatcaatcg gattattttc tttttcatat ttcctcctga aaaaaatcta tattcattaa
    19201 ataatagttt aacataaaaa caaaaaagca ccgaaatcca acataggatc tcagtgcttt
    19261 taaatagtaa aaattattta acagttacag atgcgccaac ttcttcaagt ttagctttca
    19321 attcttcagc ttcttcttta gatacgcctt ctttaactgg tgcaggagcg ccatcaacta
    19381 cagctttagc ttctttcaag cctaaaccag ttgcttcacg aactgctttg ataactttaa
    19441 ctttttggtc tccagctgca gttaattcta cagtgaattc tgtttgttct tcagcagcag
    19501 cagcgccacc agcagcagct acagctacag gagcagcagc agatacgcca aattcttctt
    19561 caatagcttt aactaggtcg ttaagttcta ggatagttgc ttcttttaac tcagcaacga
    19621 tgttttcaat gttcaatgcc attataattt cctccgttta attgttcttt tttatttaat
    19681 gctttatgcg gcaattaagc tgcatctcca tcttctttgt ctgccactgc tttgacagcg
    19741 taagccacgt tgcggactgg cgcttgtagt acagatagca acatagatag aagtccttcg
    19801 cgatttggca attttgccaa tgctgtgatt tcttccaatg aagagacttt accttcgatg
    19861 ataccgcctt tgatttctaa tgcttttgct tctttagcaa attcgtccat gattttcgct
    19921 ggtgcgacta catcttcgtt actgaatgca acagctgtag gtccagtgaa tacttcgtcc
    19981 atgccttcaa ggccagcttt tttagctgca cgtgaaagaa tagaattttt gataactttc
    20041 atttctacgt tagcatcacg aagttgttta cgtaaacgtg ttacttcatc aactgttaat
    20101 ccgcggtagt caacgactac tactgatgca gctgctgtaa atttttcgct tacttcatca
    20161 acgattgctg ctttttttgc gataattgct tcactcattt ttgagtttca cctcctgaat
    20221 tttgatggaa gagttttttg cactgaaaag gcaagtcact tttatagtta ctttgctcaa
    20281 ccagtattat tcatcaccta ttcacttcgt tcatagtgat tctactcgct ttgctcgaaa
    20341 ggaaactcct ttcgctttgc tcaaccagta ttgttcatca tctcttcact tcgttcatag
    20401 tgattcacaa taaaaaactc tatgccaccg cagacataga gggactattg aatatattca
    20461 ataaacgtcc tcggtaggaa attaaggctt gatgccacct actgtcttcg gtacaagtta
    20521 attattaacc tcaactagct tagcatgcta aactagttga gtcaagaatt atttttcagt
    20581 tggatcaatc aacattctaa tatttaatta gaaagaagct tggtcaacat ggataccagg
    20641 tccaaatgtt gttgtgatcg ttacattttt catgtattga cctttagctg ctgatggttt
    20701 agcttttagc aatacatcat ggattgctgc aaagttttca actaattttt catcgtcaaa
    20761 tgaaactttt ccgattggta catggatatt tccagctttg tccacacggt aagttacttt
    20821 accagctttc acttcgttta ctgcttttgt tacgtccatt gtaactgtac cagttttagg
    20881 gtttggcatt aagccttttg gtcctaatac tcgtcctaga cgacctacag tagccatcat
    20941 atcaggtgtt gcaacaacta catcaaagtc aaaccatccg ttttggattt tttgaaccat
    21001 gtcgtcgtca cctacgaaat ctgcaccagc tgcttcagct tcttttgctt tgtctccttt
    21061 agcaaaaact aaaacagttt gtgttttacc agttccgttt ggtaatacaa cagcaccacg
    21121 gatttgttgg tctgcttttt tagggtctac gttcaaacgg tatgcaactt caactgttgc
    21181 atcaaatttt gcaatgtttg tttcttttgc taaagctact gcttcggcaa caggataaac
    21241 tttacttgaa tcaacttttt ttaatgcatc ttgcattttt ttgctctttt tagccatttt
    21301 ggttcctcct tgattgtggt tataacggtt gtacctccca cgtgttctat atctttcaat
    21361 atagagccaa ctgagaagct gctgtcttag ggtgtataga ttattctaca gtgatcccca
    21421 tgcttcgtgc agttccttca accatgcgca ttgctgcttc aacgttagct gcgtttaggt
    21481 cttccatttt taattcagcg atttctctta cttgatcgct agaaactttc gcaactttat
    21541 ttttgtttgg ttcgcctgaa cctttttcga ttttagctgc ttttttcaat agaacagcag
    21601 ctggcggtgt ttttgtaacg aatgtgaatg aacggtcttc gtatacagaa ataacaaccg
    21661 gaatgatcaa acctgcttga tcagctgtac gagcgttgaa ttcttttgtg aatcccatga
    21721 tattgatacc cgcttgacct agtgcaggac ctactggtgg agctggagtt gctttacctg
    21781 caggaatttg caatttaacg attttttcta ctttctttgc cacgagacat acctccttaa
    21841 gtccgtgatg tggttaattg gagatgcata tttctcctcc cacgttttat ctgatctgaa
    21901 aaaatccaga caaataatgt gcaaaacaat gcacactggt acagtatagc aaatttattt
    21961 gttttttcaa gatttttttc tgctattttt acattttgct tgaataaacg ctaaatttaa
    22021 tataaatttt atatctcatt ttcaattatc cgcataaata ctatacattc aagtatttta
    22081 aggtatagaa aaacactcat tttaccacga atttaccacg attgaatgag ccttgatatg
    22141 acacaaaaaa caacatggga gatagcacaa atatctgtgt atctcccgtt tttcattcat
    22201 gtaatatgat tactttactc tgatttcaaa tttcaagatt gctttcatca tttctgtatg
    22261 aatttgacct tttaattctt catcaacctg catagtaatt ttaccacttt cagagtggta
    22321 aaacggacgc aagcataatt tacaaatata agcgtcataa aacttcaaaa tctctcttat
    22381 ggcagtttca tttccagcag acgctttaca tattactttt aatgtagggt agccatattc
    22441 ttttttcatt gtttcaattc ctttctttca ataatgtttt taatttcttc aatccgttac
    22501 ttcttctgcg ataaatagca gaacgtgata catgatataa ttctgcaatt tcttggtcgc
    22561 tcatgctttg aaaatagcgt aatagaatta catcacgctc tttactcgat aacttatata
    22621 aagcaacagc aagtttctcg tttgatattt gaattgtgaa attcaagact tcaaaagaaa
    22681 tataatcaca agaatattta tcctcagtta cacattcaag atttttcgta tctgataatt
    22741 catagaataa aacctcacgc ttggaacgtc ttgcaaactg tctatttctg ctttttactg
    22801 tgcatttaat aacagttatc atcaaagcat taaactgtat tctgacgata tgctcgaaag
    22861 aagatgtgtt cataatctca ccccctttct gcatacgccg aaatatataa acgggaatac
    22921 cgcctatacc ttttggtaat gtaaaaaaga aagcaagaaa aaagcccgca caaaacatat
    22981 agtctgcacg agccttcaaa atttccttat attcagatgt tgataattgc catattagac
    23041 tgtttaatag tgtgcttaaa acaatttatg acaatattct tcgttaatat tttcatttag
    23101 tttctccttg aaatcaacgc attattttta ttttttctgt caattcaagt atttttgcat
    23161 ggattgaatg gattgtcttt aaaaattcat catcattttt ccctgtaggg tcattcaacc
    23221 cccaatcctc tctatatttg catggtattg taggacaatt cacattgcac cccattgtaa
    23281 tcacaatatc tacctgtggt atttcagata acaatttaga atattgtgtt ttctccatat
    23341 ctatctgata catttctttc attagacgaa ctgcatcttg attgatttgt ggctttgttt
    23401 ctgtgcctgc ggaataacta tcaaaaacat ttgaagaaag atacttgccc aatgcctccg
    23461 caatttgact gcgacaagaa ttatgaacac agacaaaggc tactttaggt tttttcataa
    23521 atgaattacc aacctttctt tgttttatta tttgctcaat aatgctttta cttcgtctac
    23581 ggtcaatact tttccatagg atattacttc gccattaaac accaatgcag gtgtagacat
    23641 tactccataa ctagcgattt ctgcaaaatc tgtaatgtgt tcaatctcat aatctaattg
    23701 aagttccgat attgctgttc tagttgcttt ttccaattcc atacattttg cacagcccga
    23761 acctaatatt ttaatgcctt gctcttgttt cttgttttct gcattttgca ttgtttctga
    23821 tgtgcaattt ccaccacaac aacatgattt tgtttctttt ttctttccaa ataatttcat
    23881 ttcttattcg ctccttttaa ataaataatg tactaaaaat attaaagaga taacctacaa
    23941 taataatgcc aaacgtacaa atggcaataa aaagggtcaa tagtttaggc tttactgcct
    24001 ttttcagcat tattaaagac ggcaaactca atgttgttac agccatcata aatgataaaa
    24061 ttgtacctaa ttgtgcaccc tttgaaagta atgcttctgc tactggaatt gttccaaaaa
    24121 tatcagcata cataggaact ccgacaagag tggctaaaat aacaccaaac gggttattac
    24181 tgcccaagat actttcaatc catgtttcag gtatccagtt atggataact gctccgatac
    24241 ccacaccaat caaaatgtat ggaaatactt tcttgaatgt tcctacaact tgttcttttg
    24301 catattgaac tctatccttt ttggttaaag taggagaact aatatcgaca ttacttgcat
    24361 ttttcacgaa atcttccaca tatttttcca tgtgcatttt ttcaattaaa gtaccgccaa
    24421 ttacagcgat tatcaagcct acaatcacat aagcaaaagc aactttagcc ccgaaaatac
    24481 tcattagcaa tacaagactc cctaagtcaa ccataggcga agaaatcaaa aaggaaaaag
    24541 ttactcctaa cggtaatcct gcacttgtaa aacctataaa caacgggata gaagaacacg
    24601 agcaaaatgg tgtaactgtt cctaacaatg cggatatgat atttgcaccg ataccatgaa
    24661 atcgccctaa aattttttta cttcgttcgg gtggaaaata actttgaata tacgatataa
    24721 agaaaatcag taaacataac aagattgtga ttttgattac atcgtataaa aagaattgaa
    24781 tacttccacc taaacgactg ttaatgtcta gccctagcaa tgatagccca ttaccaatta
    24841 agccattcaa ccatttcata cctaatattt ggtcttgaaa aaatagccat attgaattta
    24901 ctacttccaa taaagtccct cctttacatg acatatcaat tttttttgat gtgataaagt
    24961 tttataaaag ccacctttcg atgaccttta tttgcaacaa attgattgat tttcagcatt
    25021 tggtgttgtt agttctaaca atcgttttga cgcatattca cttccacttt tacttaggct
    25081 gtaatgagtc cattttccct cttgtcttgc attaacaata ccagagtcgc aaagtatttt
    25141 catgtggtag gataaagccg attgtcctat gttcatgttt tcaatcagaa cacacgcaca
    25201 tttttctccg ctctttaagt aatctaaaat cgtaagtctt tttgtatcac aaagagcctt
    25261 aaaaattttt gcgtctttag aaaaatcatt cattattatc actcctcaca tcaaataatt
    25321 ttgatatatt tatcataagg cttacaacat gatttgtcaa cacattacat caaaatattt
    25381 tgatgtatta ttcttttttc ctcttgatta cccataacag aaaagaaaaa gccctgtcaa
    25441 gagcttgcca ccattggtgg tgctttgcac tctttactgg gctttttgtt tgctgtatct
    25501 tccatgcaag aggaaaaaga ttattcgtcc tcatcatcaa aatggttatt ccttaatact
    25561 tcccgtaata attccatatc ttcttttgaa ttagggttta tcattttgac cacttgataa
    25621 ggtgtcctat atttatgtcc ctctatgctt tccccaaaca tatccatgct gtataaatca
    25681 tcatagcggt ctaacagttt ttgaaacttc aaatgctgga taaattcttt gattgggtaa
    25741 aggtcggacg cttcaatgtt tgttccattg atgtagtcgg taatatattc tcgtaacttt
    25801 tctaattcat attccagcca ttcttcctcg ctgtcaaaga agttgtcata atgtccatgt
    25861 tttaattctg catttagacg ttcttctgtt cttaaaaact gacagacttc tttttcggac
    25921 tgattgacat atttccattc ccccgacaac aattcattcg gcgtaacgtc cagcacttcc
    25981 attatctttt ccagcgtttc ataagcagga tagttcaacc ctctttcaat cttggaaagg
    26041 ctctgcatat tgataccaat tttgtccgca agttcctgtt gtttcattcc cctaaatttt
    26101 cttatggtct gtatattatt tcctaaatgg cttattttca ttgcataacc ctccataact
    26161 cgttatatct tcgctgctat aagtctatca tgcttaatat gatttgtaaa gataaaaaaa
    26221 gatattgaca aataagcata taagaattac tatgttcttg taatcaaata agcacaatag
    26281 aatgaaatac aaaaacattc aaaagaaaat atctatctga ttaaacccgt agatatgaat
    26341 atcggttgaa atggaacttg tttgaaagga caaacacagg acttgccgac agagcattgt
    26401 aggactgttg gcgtgcgtca gcaacaacgc catgacggct tgccgtcaaa ggacggaaat
    26461 gctttgtcgg ctggcgaact tgtgagcctt gtctgtgccc ccgttatcta acaggggggc
    26521 actatttcac aaaagacaag tcaaaaacca tacaacccca aagctgtaag gagtaatgag
    26581 gagttttaag aagatagtaa cttcacattc ttttgaatag tggtttacag gtatttcaag
    26641 gctgttgtgg gattggagga atacaatgaa caatttagaa tttgcacttg aaatgaaaaa
    26701 caaacgtctt gcaagcggtc tttcacaaag agaacttgca ggtcttaccc atgtatcaag
    26761 atatagtatc aaccgctttg agaacggaaa agccaacgcc agcaaggaaa cacaaaatat
    26821 tatcctgcgt tgtctgaatt actatatctg cgataagcct ttttatctgc tcgttgatta
    26881 tctgtctgtc cgtttaccaa cgactgacgc attagaagtt atccgaaagg tgcttggcat
    26941 gaaagcagat tattttatcc attatgacta tgggtattat ggctataagg agcattacgc
    27001 ctacggggaa atcaaggtca tggcttctga tgatgaacac atgggcgtat ttttggaatt
    27061 aaaagggaca ggctcacgca acatggaata tgtcttacag gcacagaaca gggattggta
    27121 ttcattttta aaccgctgtc ttgactgtgg cggtgttatc cgacgttttg atttggcaat
    27181 caatgatatg tgcggtctgc tggatattcc cgttttgtct gaaaaatata aaaatggcgg
    27241 tgcggaatgt cgctgtaaaa actatgaaaa tgtacaaagt ggaaaattaa gtgggaaaaa
    27301 ccgcaattta gcaagtaccc tttatatcgg ttcaaaggca agcacaaaat atttctgcct
    27361 gtatgaaaaa caaaaggaac aggcaacgaa aaagaaacat accgatatta tcaaccgttt
    27421 tgaaattcgt ctgcgtgata aaaaggcagt acaggcagtt gaggaattgt tattgacata
    27481 taaccctcat gggctggtgt tttacctcat tactgatttt gtgcaatttc ccgactatcc
    27541 gttgtgggag atattcattt cccatgacag tttacctttt gaaatgaacc ccgtacctgt
    27601 caatatggaa cgcactctgc aatggctgga aagacaggtc atgccgtcaa tcgtgatgat
    27661 agaggaaatc gacaggctga caggctcaaa ctacatgaaa atgattgatg aatgtaccca
    27721 cctttccgaa aaacaggaaa tgcttgtaga acagatgtgt acggatatag cagatgtaat
    27781 cgaaagtgag ggggtgtttt atgagtaatg cacaggatat tcccgtatgg gaaaaatata
    27841 cccttaccat tgaagaagcg tcaaagtatt tccgtatcgg agaaaacaag ttaagacgat
    27901 tggcagagga aaacaaggac gctggctggc tcattatgaa tggcaaccgc atacagatta
    27961 aacgccgaca gtttgaacag gttattgata aattggacgc aatctaatgc aaatgagcct
    28021 tgtatgtgtt atgataagca caagtcatat caaggctctt tccaacaagg aaaggagcag
    28081 acaccatgag agaaaaaaga cgggataaca aaggtcgtat cctgcatact ggagagagcc
    28141 aacgaacaga cggaaaatac ttatataaat atgtagacgc atttggaaac acaaaatatg
    28201 tgtatgcttg gagattgaca cccacagacc cgacaccaaa gggaaaacgg aaaaaaccct
    28261 cacttcgaga actggaacag cagataagac gggatattga ggacggtatc gacagcgcag
    28321 gcaagaaaat gacgctttgc caactctatg ccaaacagaa cgcacaaagg gcaaatgtga
    28381 agaaaagcac acaaaaacaa cgggaacaac tcatgcggtt attgaaagag gacaaattag
    28441 gtgctaggag cattgataca atcaagccct ctgacgctaa agaatgggcg ttacgcatga
    28501 aagacaaggg cttttcctac aataccatta acaaccataa acgctcgtta aaagcgtcat
    28561 tctatatcgc catacaagac gattgcgtaa ggaaaaatcc ctttgatttc aaattaagtg
    28621 aagtcctaga aaatgatacc aaagagaaag tcgcattgac agaggaacag gaacaagcct
    28681 tactctcatt catcaagacg gacaatgtgt atcacaaata ttatgatgat atgctgatat
    28741 tgttaaaaac aggacttcgt atctcggaac tgtgcggact gacagtagcc gatattgatt
    28801 tcaagaatga agttgtgatt atcgaccacc agttattaaa gaacaaggaa cagggctatt
    28861 atattgaaac gcctaaaaca aagagcggaa taaggcaagt gccattaagc agagaaacga
    28921 tacaggcatt tcaacgggtt atgaaaaaac gcccaaaggc agaaccattt gtgatagacg
    28981 gacggagcaa ttttctgttt gtcaatcata agggtaggcc caaagttgcg attgattaca
    29041 acgccttatt tgtccgaatg gtaaagaaat acaacaagca ccacaaggac aatcccttgc
    29101 cacatatcac accgcatacg ctacgccata cattctgcac aaggctggca agcaggaaca
    29161 tgaacccgaa agatttacag tatatcatgg gacattcaaa catcagtatc acaatgaact
    29221 ggtatgctca tgcgtccata gataccgcaa aatcagaggt tcagcgtcta atcgcataag
    29281 aagtatttac cacgatttta accacgattg acagggaaaa tataagaaga tagatctaga
    29341 tatgtgaggt ttaccacaaa aacaaaatgc ccgtagagcc tataaaataa ggctttgcgg
    29401 acatttaaga agatataaaa agatagtcaa aaagacatat ataatttatt ctaaaaaaac
    29461 aacaattttt cgttctcatt tcttctttta tgttacactt gcgctagcat ggttcaacag
    29521 atgaaagcaa acaataacac ggttttttag acccttgtac aaagattcct acataacaaa
    29581 atgaggtgga gaaaatgaaa gatttaaggt atagaagtgt ctttgatatt attggacctg
    29641 tgatgattgg tccaagtagc tctcacactg ccggagctgc tagaatcggg aaaatcatac
    29701 gtagtatatt tggcgaacag cctgattctg tagatattta tctatatgaa tcatttgcaa
    29761 aaacctatcg ggggcacgga acagatattg cattagttgg cggcctgtta gggatggaac
    29821 ccgatgatga acgtttagct gattctttga aattggcata tgaagccggt atggaagtct
    29881 tgtttgttcc taagagtgaa aaagccgaac atccaaactc tgtaaagatg cttctaaaaa
    29941 aaggaacacg aaagctttca gtcactggca tttcgatcgg cggtggcaat atccagattt
    30001 ctgaattgaa tggattcaaa atttctttaa gcatgggtac tcctaccttg atcgttgtcc
    30061 accaagatgt tccaggtatg attgctaaag tgactagtat actttctgag accaatacga
    30121 atatcggcac tatgacagtg acacgggaat caaaaggtga aaatgccatt atgataattg
    30181 aagtagatga tcctcaagta gaagaaacgg taaaaagact caaacagctg ccaaatatcg
    30241 acagcgtaaa ttatttcgat taaggagcta aaaaaatgtt tttatcgatt gaagaattag
    30301 tagatcaagc aaaagcttat ccaagtgtag cagaattgat gattgcagtt gaaatggaaa
    30361 tgagtggccg ttcccgtagt cacatcatcg agatcatgga aaaaaacctc tcagtaatgg
    30421 aacaatcaat caacgaggga aaagaaggtg ttacctccgt cacaggaatt accggtggtg
    30481 atgcaaaaaa attagatgat tatttaaatg aggcagactt tttaagtgga gaaacaatct
    30541 taaaagctgt acgcaatgct gtagctgtta atgaagtcaa tgcaaaaatg gggttgattt
    30601 gtgctacacc gactgccgga agcgcgggtg ttgttcctgg tgtgctgatg gctgtccgag
    30661 atcggcttga tttgacacat gaacagcaat tggaattctt atttaccgct ggcgctttcg
    30721 ggttagtcat cgcaaacaac gcttctatca gtggtgcaga agggggatgt caagcagaag
    30781 ttggttctgc aagtgcaatg gcagcagcag ctttggtttg tgcaaaaaat gggactcctc
    30841 atcaagcagc acaagcagta gccattacat tgaaaaatat gatgggactg atttgcgatc
    30901 ccgttgcagg cttagttgaa gtcccgtgtg tcaaacgtaa tgctttaggc tcttctcaag
    30961 cttttatctc tgctgatatg gcattagcgg gtatcgaaag tgtcattccg ccggatgaag
    31021 tcgttgctgc aatgtaccaa gtcggacgtc aaatgccgtc gattttcaaa gaaacagcag
    31081 aaggtggctt agctgttacg ccaactgcta aacgacttgc ggaagagatt ttaaataaga
    31141 gggcgtgata aagctcctta atttcgagta ttaagagatt agttatcttc ataacaaaaa
    31201 acgaagagag gactaattgc ccttctcttc gttttttatt aatccatcaa tttcgtattt
    31261 tttacttcat cgatcgtttt tgttcctgca agttgcatcg tgatcgacaa ttctttgttc
    31321 aggtgttcaa aaacagatgt tactccttct gccccaccta gattcaaccc ataaatcact
    31381 ggtcggccaa tcgcaactaa gtcagcacca cttgccagag ctttgaagac atgctctcct
    31441 cgacggacac cagagtcgaa aatcactggt acacgtttgt tgactacttc ggcaattttt
    31501 ggcaatactt caaaggacgc aggacctcca tctagttgac gtccaccatg attggatacc
    31561 cagataccat ctgcccctgc agagatagcg attactgcat cttctggcga ttgtattcct
    31621 tttacgataa ctggaagatg ggtgatttct ttgattgttt tgatgtcatc tggtgtcaag
    31681 ccttgttttg cagctgcata aatttctgcg attccttttc cttcgccatt accacttgca
    31741 ctttgttcgc tatacgcagc taagttaggc attggtaatg gaaattggaa ttgattgatg
    31801 acgtcttctt cacggtaacc accgagcgtt gaatctgccg ttaaaatgat agctttagcc
    31861 cctgcttcta ctgctttgtt caaaatgaac tcattgaatc tgtcgtcttt actcatatat
    31921 aattggaaaa actgcggtgc gcctggcgct gcatcggctg cgtctttgat tgtcgtattt
    31981 gcataagtgc tgatggaaaa gattgatcca gcagcagcta ctcctttagc tgtatcagct
    32041 tccccttttt catgtgccaa tccttgggcc gctgatggcg cttggataat cggtgtatct
    32101 aattcaattc cgaaaacaga tgtatgcaaa tcggctgagt cgattccgcg taatatccgt
    32161 ggcatgattt tctttgtatt aaaagaagtc gtattttctt tcatcgtcca ctcatcttca
    32221 gaaccaccgc gaatatagcc aaaagctcct gcttccatgc gctcttttac acgaccttct
    32281 agttaggcaa tgtttacaat atcgattgga tgttcttcat tacttgtatg ataagtcata
    32341 gtagatcctt ctttcatctt cttgattaca tataaatcat actaaactta caaaaagtaa
    32401 gcaaacattt actctcagat acttttatcg ctattctttc caaataaatc gacttacaag
    32461 ctgatctact tcttggtttt catgaagcat actatgagag gcattttcac ctgtcactac
    32521 tttttcctct atttcatttc cgcgttgctt caacaacgca tagaccgcta atgcactgtt
    32581 taacggtact gttccatcac tcaagtctgt ttcatctagt tgaccagcaa tcaagaaaaa
    32641 tcgagtggtt ataggaatat taccgatcat ttgctggtaa tcttgatagc ggttagaaac
    32701 gacagcaggc ccctttgcta actcatctgt cagagactgc gcactatcat cgacaaaatc
    32761 attaaaaggc gcgccaattg ctgttaatgt ctgaacttgt ggcaatgacg tatcctgtcc
    32821 atacgttcct aaatagcgta gtgcactaac tcctcccatg gaatgcccca ctatgttgac
    32881 tttatctatg ccgtagtttt ctttcagata caaaagacaa gttttgatcc attctgcttg
    32941 attccactcg ttatttttat tatcttcaaa caatacttgg accatcggat tgtctgcttt
    33001 tccagacaaa tctcctgttg cttgtatttg tccatctgtt cctactgtta aaaccaattc
    33061 ttttactgct tgctgatttt tttccaaacg ccgcaacatc cctttaaagg aattttctgt
    33121 cccgctgtag ccgtggaaaa aaagtgttgg aatagtggtt ttttctatca ctttactgga
    33181 ttctgccgtt ttttctttat cagaaacttc agtagtattt atcttcgtcg tctggcaacc
    33241 acttaacgca aacaaactga taaggactaa agctgctgca gttgcacgtt tgcataaatt
    33301 tttgattggt tctgtcatac acctcttcct ttccatatag aaaaaagaaa gacttgaaag
    33361 gccaatcata tgatcgacat ttcaagtctc tttattaatc gattttatct acttgatcga
    33421 aatcaagttc tgtactggtt tcacgtccaa acatatcgat gttgactttc aatttttctt
    33481 tttctccgtc aacttctgtt acttgtcctt ctaatccagc aaatgcgcct tcgatgattt
    33541 tcacgacttc gccaacttct acttctagat cattttgtcg caggctcata ccgatagaac
    33601 gcaagatatg attgacttct tctggaagta atggtgctgg tttgcttccg gctccgtgtg
    33661 aaccaacgaa tcctgtcaca ccaggtgtgt tgcggacaac ataccatgaa gcgtctgtca
    33721 tgatcatttc aacaagtacg tatcctggga atgttttatg aacgatttct ttttctttcc
    33781 catttttgac ttcagtttct ttttcttcag gaacaacgac acggaaaata aagtcggcca
    33841 ttttcatact ttgcgcacga gattcgatat ttgcttttac tttgttttca tatcctgaat
    33901 atgtatgcaa gacataccag tttttttcaa aagattccat tgaaggctcc tcatttcatt
    33961 gtaaaataaa aaaacctcag tacacccgaa gtttttcttt cgcttcatta tagcactaaa
    34021 agtacgttgt ggctagcgag ttattttaaa atccagccaa ataagctttg gatggctgta
    34081 tccattacgt aaaacatcaa tccgaaaata atagaagttt cgatgactac taacgtatct
    34141 ttacggagtt gttttctgct tggccaagtt acttgtttga tttcatcttt tacgcttttt
    34201 agaaatttca tgagacaacc tcccttgcta ctgtttattt cgtttcttta tgtaatgtat
    34261 attgattaca atatttacaa aacttattga tttccaaacg ttcgccgctt tttccttcac
    34321 tgactgattt tgtgtagttg cgagacccac aaacggaaca agcgagtgct gcttttttct
    34381 ttgccattta cttctgcccc cttgctgaga atcagactta tacttcataa cagtatcacg
    34441 taacttttta cttgtcaatt tgtgaaataa actatctatt gttttggata aaattatgat
    34501 atgattaatt tgaaattaag ctaaaatgag acaattggag gtatagcgat gctattaaaa
    34561 acacttgtat ataagaaaaa agatctgaca accgttacag agactagtac attagaagaa
    34621 gcattaaaaa ttttagaaac ttccggatac cgttgtgtgc cgatcttaga ttcttcaggt
    34681 aatattttcc gtggaaacat ttacaaaatg catatctatc gtcataaagc aaatggcggt
    34741 gatatgacgc ttcctgtcac tcatcttttg aaaaacgcta caaagttcat ttacctggat
    34801 acttctttct tcaaagtatt ctttacgatc aaagagcttc cttatattgc tgtccttgac
    34861 agtgaaaatc atttttatgg tattttgaca catagtacgt tgcttaatat gctctctcaa
    34921 tcttggagcg tagaacaagg aagctatgta ctgacaattg cttccacagg aaagcaagga
    34981 gatttggctg ctattgcaaa aatcatttca aaacacagca gcattgccag ctgtattact
    35041 cttgatatcg aaaaagacga atacattcga cgtacattga tcacgctacc ggctaacact
    35101 gaagcgaaag tatgtgaaac gatcgtcaac cacttagaga aaaagaatta taaagtggta
    35161 gaaattgaaa atttgcaaaa ccaagaagca taatcaaaaa gcgaaaaaag aaaacagcta
    35221 atctgctttc ttttttcgct ttttataatt agacacgctt ttcaaaatca agcgcgcgat
    35281 ctaaataata catcaatggt gctgaaacag ccatgacgat aaactcactt aatgcaagcg
    35341 tgccataggt tgtccaaaat gcttggccac ctgttggaac taacatataa gcaatcagtc
    35401 ccatgctgat ggtaaagaag atcgtgttca atactaaacg catctttaca tttttcactt
    35461 tgttttgcaa taaagctgtc aggcctaaag aaagcaatgt ttggccgcca ccaaaaacca
    35521 catccatgat accaaaacca aagaaggcat tatagacgat gaccccgcct aaaactcccc
    35581 aaagcaattt acggttaaag acaactaaat gattcaaact ttctgaaata cgaaactgga
    35641 tcggccctga ggatacaggc gcaactaaaa tagttaacgc tagataaaga gccatgatga
    35701 tgccatttac aacgattacg gataaccgtt gtctgctttt cgttttttgc attttctctc
    35761 ctcttttctc ctagtttttt taacgcggga tggttgatga accgcggtta tgtgaaacaa
    35821 acaataatta taacagactt ttcatttatt tcaatccgtc ttatctgctt ttaaaaaagc
    35881 aaaagcttaa acaatgtaaa cgtttgctaa aaatcgtggg atatggtata aagtaagtgt
    35941 aaggggtcat aagaattccc agtaaagcag acgaagtagg atctgcaaga acagaattgt
    36001 tgtgaaactt aaataaataa aaaaggatgt gggaatttga gcaacctctt actagagaac
    36061 ggtctagcaa gcagctgaag atctaagtgg acatgccgaa ccttggaact tctccttacg
    36121 taagtaatgg atcatgcgct tgctaggctt ttttgtgttt ttcggtcttc ggaccttttt
    36181 cattttccta gacttctgct ataataacca agaattaatt ttgtaacaga tttttttgaa
    36241 aaaacaagtt tctttcattc aaaaaataga aaaagcttgc taattattgg ttaaccagca
    36301 aacaggagta ttaaaatatc ttgttttatt gaatagcagg aggaaatcat gaaattccat
    36361 tatcatatat tgatcaatcc atcggcaggc agcggaaatg gctataaagt agcagaaaga
    36421 attttaccgg tattaaaaaa taagcatatt gactatacac ttcattatag tgaatacaaa
    36481 ggacacgatg ctgagattgc tgaaactttg gcaaaagaaa cattgattcc ttggatagaa
    36541 gaagaacaga aaacaatcga tactttcccc cttttgatca tcgtaggagg tgacggcacc
    36601 ttacatcagg tacttgatac tttctatcag atggaagtag aattcccagt tgcttatatt
    36661 ccagcaggtt ctggagatga ttttgctcga ggggcaggcc ttccaaaaaa tcccaaaaaa
    36721 ggacttcagc tgattttagc agctcaatca ccagaaaaag tccacataat ggcttatgaa
    36781 gaaaaaataa gtgaaaaaaa aggaatagct gttaataatt tcggtatagg attagatgca
    36841 gctatcgtcc atgcgacaaa tcattcttcc acaaaaaagc gattgaacaa atacaactta
    36901 ggatcatttt cgtatttatt ctctattctc cgtgctttat tcactcaaaa aggatttcca
    36961 attctggtag atgcaggagg aaaacaatta agtttttcaa acgcattttt atgtacagca
    37021 acgaagcatc ctttttttgg cggaggaatc gcgattgttc caactgctga tgcgtctaaa
    37081 ccagtcattg attttgtcgt ggtcgaacga atcaatttct ttaaaatctt gtggctcatt
    37141 cttttactta tacagaaaaa acaattgaaa tcgaaatatt tccatcacta cacaacaaac
    37201 agattacgta tcgtttcaac aacaccgcaa catggtcaag aagatggaga agaaatggaa
    37261 aaacgacctt ttgatatcac aataaccaca actaaacagt tgttttggtg ttaataaaag
    37321 aggttttgtc agaagctaca tctggaacac cgtttattcg gaaaaaagga ctgtgacacg
    37381 acttttgtca cagtcctttt tttactatgc agttaattct ttgcccaatt gggttgccat
    37441 cgatagtttc tttctttacc caaaataaat tggatcattg aaaacaccgc agcaaaagca
    37501 actaagacac aaccattgaa ccaaaaagat tctgcaaatg tttgattgta actgagtgtt
    37561 tcactgatca agttattatt ttgaacaaac caatatgttg gtgtgaatgc cgctattttg
    37621 ctaaccattt ctggcaagta ttctgctggt acaaacaccc caccgataaa gcaactgcct
    37681 gtgataaaaa tatctttgat cccaccgctg gtttctgagt tttttaccaa tgaagtgata
    37741 caagccgaaa agctaatgat cggcaataaa aacaagaaag tatttaaaat atgataccat
    37801 accgttatat cccacgaggt gtggatcact aacagaacca ttagaagtaa gaagccactg
    37861 aacaacagca gcgagtaact aatcaaacta aaggttactt tgcgattcaa gtttcgtttg
    37921 gtaatgggcg cactctggtt tcgtttactg atctctttgc gattaaatgc tagattgatg
    37981 acgctgaaac cgctaaaaat ggatagaaac aacccataag acaagaaatt aaaggttgtg
    38041 cccgtcaaca gctttttcat ccgctgacta tatcccgcac ttagctcaac ttctccagta
    38101 accgagagtg ccatttgtgt ctgttccata gctgcatcga ttgttttatc ttcttcttga
    38161 aacagaatca gtgtttgcaa aaaggtgttc acttgctgtg taacaactgt tttagaaaag
    38221 gcatctgggc gactctgtat cttgatcgtc ggcatctttc ctacttcgac ttgttcggta
    38281 aagtctgcag gcagatgtaa gataatgttt acacgattaa aataaagagc atcatcgatt
    38341 tctttttgtg aggttccatc caatgtaacg actgtttcct tgtcattcaa atatttacga
    38401 aggccatcga ctacaggcga ctcttcttca ctaatcaagg cgattttcgc atcttgcaga
    38461 gaggtatttt caacatcaat attttgcgca aaaaagaatg tggcaacaac agtgataatt
    38521 acacctatca gtaaattcga tttatttgct ttaagcaatg tccaaatcgt tttataaagc
    38581 gtcataacgc acccctcttt ccatccaagc atttcctaac gcaaaaagga aaataaatcc
    38641 gatcaaccat agaagattgg tataaaaact agccaaatca gtgtagaaat aaagttt
//
LOCUS       DAJSBL010000023        37940 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.023, whole genome shotgun sequence.
ACCESSION   DAJSBL010000023 DAJSBL010000000
VERSION     DAJSBL010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37940
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.023"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            137..310
                     /locus_tag="ONT85_001392"
     CDS             137..310
                     /locus_tag="ONT85_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2378376.1"
                     /translation="MMKRLYYSLIITIGYLIVSNLGNMVFGISKEFSWTTTLWESLFF
                     FIFVFLLQNYRKK"
     gene            950..1423
                     /locus_tag="ONT85_001393"
     CDS             950..1423
                     /locus_tag="ONT85_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HCR2378377.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            1432..1659
                     /locus_tag="ONT85_001394"
     CDS             1432..1659
                     /locus_tag="ONT85_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR2378378.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
     gene            2295..2666
                     /locus_tag="ONT85_001395"
     CDS             2295..2666
                     /locus_tag="ONT85_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2378379.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
     gene            complement(2922..3164)
                     /locus_tag="ONT85_001396"
     CDS             complement(2922..3164)
                     /locus_tag="ONT85_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HCR2378380.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(3196..4098)
                     /locus_tag="ONT85_001397"
     CDS             complement(3196..4098)
                     /locus_tag="ONT85_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HCR2378381.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            complement(4111..4299)
                     /locus_tag="ONT85_001398"
     CDS             complement(4111..4299)
                     /locus_tag="ONT85_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HCR2378382.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            complement(4313..4876)
                     /gene="amaP"
                     /locus_tag="ONT85_001399"
     CDS             complement(4313..4876)
                     /gene="amaP"
                     /locus_tag="ONT85_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HCR2378383.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            complement(4914..5807)
                     /locus_tag="ONT85_001400"
     CDS             complement(4914..5807)
                     /locus_tag="ONT85_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR2378384.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKDAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            complement(5885..6807)
                     /locus_tag="ONT85_001401"
                     /pseudo
     CDS             complement(5885..6807)
                     /locus_tag="ONT85_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303839.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
     gene            complement(6856..7206)
                     /locus_tag="ONT85_001402"
     CDS             complement(6856..7206)
                     /locus_tag="ONT85_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HCR2378385.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            complement(7239..8141)
                     /locus_tag="ONT85_001403"
     CDS             complement(7239..8141)
                     /locus_tag="ONT85_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HCR2378386.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            complement(8134..8991)
                     /locus_tag="ONT85_001404"
     CDS             complement(8134..8991)
                     /locus_tag="ONT85_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HCR2378387.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            9325..10134
                     /locus_tag="ONT85_001405"
     CDS             9325..10134
                     /locus_tag="ONT85_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCR2378388.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            10174..10671
                     /locus_tag="ONT85_001406"
     CDS             10174..10671
                     /locus_tag="ONT85_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HCR2378389.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(11056..11129)
                     /locus_tag="ONT85_001407"
     tRNA            complement(11056..11129)
                     /locus_tag="ONT85_001407"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11093..11095),aa:Ile,seq:cat)
     gene            11316..11639
                     /locus_tag="ONT85_001408"
     CDS             11316..11639
                     /locus_tag="ONT85_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2378390.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
     gene            11803..12057
                     /locus_tag="ONT85_001409"
     CDS             11803..12057
                     /locus_tag="ONT85_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2378391.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            12127..12372
                     /locus_tag="ONT85_001410"
     CDS             12127..12372
                     /locus_tag="ONT85_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR2378392.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            12448..12813
                     /locus_tag="ONT85_001411"
     CDS             12448..12813
                     /locus_tag="ONT85_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2378393.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(12874..13437)
                     /locus_tag="ONT85_001412"
     CDS             complement(12874..13437)
                     /locus_tag="ONT85_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2378394.1"
                     /translation="MLYIKKGIIVISFFKEYLEIINFRLKTKKESVIILVYFQGGNLM
                     IKKLFLLSILTLAMAGCSDSGLATELITKSTEATTEELKCGNDHSDYRGLNRRCMGIL
                     GRSNCKHRCSNRRRCRRSNRKIKRKIKRSNLCCASYTDVEDAVLLNHVRRCSFMSREV
                     GDEPKLEYAFSFAREDEDNPKTYDILA"
     gene            complement(14005..14205)
                     /locus_tag="ONT85_001413"
     CDS             complement(14005..14205)
                     /locus_tag="ONT85_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HCR2378395.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
     gene            14601..14750
                     /locus_tag="ONT85_001414"
     CDS             14601..14750
                     /locus_tag="ONT85_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2378396.1"
                     /translation="MTFYNKDIQYRVTLDTDLNLFVVFDKENTNHVATGVTIEQAVQE
                     LKKSA"
     gene            complement(14957..15670)
                     /locus_tag="ONT85_001415"
     CDS             complement(14957..15670)
                     /locus_tag="ONT85_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HCR2378397.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            complement(15663..16748)
                     /gene="uxuA"
                     /locus_tag="ONT85_001416"
     CDS             complement(15663..16748)
                     /gene="uxuA"
                     /locus_tag="ONT85_001416"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HCR2378398.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            complement(16765..17208)
                     /locus_tag="ONT85_001417"
     CDS             complement(16765..17208)
                     /locus_tag="ONT85_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HCR2378399.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            complement(17242..17595)
                     /locus_tag="ONT85_001418"
     CDS             complement(17242..17595)
                     /locus_tag="ONT85_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2378400.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            complement(17707..18108)
                     /locus_tag="ONT85_001419"
     CDS             complement(17707..18108)
                     /locus_tag="ONT85_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2378401.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            complement(18145..18654)
                     /locus_tag="ONT85_001420"
     CDS             complement(18145..18654)
                     /locus_tag="ONT85_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCR2378402.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            complement(18676..19533)
                     /locus_tag="ONT85_001421"
     CDS             complement(18676..19533)
                     /locus_tag="ONT85_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HCR2378403.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLKQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            complement(19551..20384)
                     /locus_tag="ONT85_001422"
     CDS             complement(19551..20384)
                     /locus_tag="ONT85_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCR2378404.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            complement(20398..21195)
                     /locus_tag="ONT85_001423"
     CDS             complement(20398..21195)
                     /locus_tag="ONT85_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HCR2378405.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNVAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            complement(21228..21512)
                     /locus_tag="ONT85_001424"
     CDS             complement(21228..21512)
                     /locus_tag="ONT85_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HCR2378406.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            complement(21509..22510)
                     /locus_tag="ONT85_001425"
     CDS             complement(21509..22510)
                     /locus_tag="ONT85_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HCR2378407.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            complement(22512..23579)
                     /gene="gnd"
                     /locus_tag="ONT85_001426"
     CDS             complement(22512..23579)
                     /gene="gnd"
                     /locus_tag="ONT85_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HCR2378408.1"
                     /translation="MRIPHFHNKIIIFSVLINVKDTRRVGKNQTKNIFVKSIYKKIFL
                     YNEGIRNGGAIMEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDIPAY
                     QEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSIRRA
                     EEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLYAGA
                     SGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWLMEI
                     MEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEEDSFS
                     AKVVSALRNGFGGHEIVEKGE"
     gene            23578..24426
                     /locus_tag="ONT85_001427"
     CDS             23578..24426
                     /locus_tag="ONT85_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCR2378409.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            25072..25278
                     /locus_tag="ONT85_001428"
     CDS             25072..25278
                     /locus_tag="ONT85_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HCR2378410.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(25480..26481)
                     /locus_tag="ONT85_001429"
     CDS             complement(25480..26481)
                     /locus_tag="ONT85_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR2378411.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(26486..28399)
                     /locus_tag="ONT85_001430"
     CDS             complement(26486..28399)
                     /locus_tag="ONT85_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HCR2378412.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            complement(28567..29073)
                     /locus_tag="ONT85_001431"
     CDS             complement(28567..29073)
                     /locus_tag="ONT85_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HCR2378413.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            29233..29673
                     /locus_tag="ONT85_001432"
     CDS             29233..29673
                     /locus_tag="ONT85_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HCR2378414.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            29832..31172
                     /locus_tag="ONT85_001433"
     CDS             29832..31172
                     /locus_tag="ONT85_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HCR2378415.1"
                     /translation="MSYSEFTKELLDILDLNLTFHEDAFRKERINDETCFVFDGTLTY
                     QPEECFHCHYQNKQTIIKWGWKKVSILLNDVSNYKTIVRINKQRFKCKHCGKTFLAED
                     SVSDRRCSIARRVKQAILELLSEPISMSLIARMKHISPTTVIRILRSLRPKTVSLNQP
                     LPEVVCFDEFKSVKNVSGAMSFVMMDGKTHQLIDIVENRQLNPLRDYFLRYPRKVREQ
                     VRLVVSDFYTPYRTLVKECFPNARIVADRFHISQHIGRAFTNHRIQVMKTFKKGDRRS
                     KHLKKYWRLLQKNAWELNGQHRYWRPSFRDHLTEAEIVDRLLYYDDSLKRGYEVYQVF
                     LSAIRRQDVPEFVALLKEDYKELPEHYQPVFTTFKKYRTEIKRALRVPYSNGPIECLN
                     NHIKVLKRIAYGFRNFQNYRERIFLYRGKYFKKTKNTTQLTKARTTTRLDKIAV"
     gene            complement(31241..32398)
                     /locus_tag="ONT85_001434"
     CDS             complement(31241..32398)
                     /locus_tag="ONT85_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR2378416.1"
                     /translation="MRTIQRKLRELNTILKNYHLSCSLKKGNPIEGEEYRIRYFFHLM
                     YWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSI
                     TYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYL
                     EEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGT
                     KKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCML
                     VRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYP
                     IDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(32401..32757)
                     /locus_tag="ONT85_001435"
     CDS             complement(32401..32757)
                     /locus_tag="ONT85_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2378417.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            complement(33055..34029)
                     /gene="sgrA"
                     /locus_tag="ONT85_001436"
     CDS             complement(33055..34029)
                     /gene="sgrA"
                     /locus_tag="ONT85_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HCR2378418.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            complement(34225..35064)
                     /locus_tag="ONT85_001437"
     CDS             complement(34225..35064)
                     /locus_tag="ONT85_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HCR2378419.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            35249..36136
                     /locus_tag="ONT85_001438"
     CDS             35249..36136
                     /locus_tag="ONT85_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCR2378420.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            complement(36720..37415)
                     /locus_tag="ONT85_001439"
     CDS             complement(36720..37415)
                     /locus_tag="ONT85_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HCR2378421.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
     gene            complement(37399..37797)
                     /locus_tag="ONT85_001440"
     CDS             complement(37399..37797)
                     /locus_tag="ONT85_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HCR2378422.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
BASE COUNT        11187 a         7561 c         6052 g        13140 t
ORIGIN      
        1 tttttcggag aattaaaaaa tacaataaat aactttagtt actttaatgc gttcttactt
       61 tatttttata ttatcatttt tcttattcac ttctttggtt tatactattc ttataaataa
      121 aaacgaggta tgtcctatga tgaaaagatt gtactattca ttgatcataa cgattggcta
      181 tttgattgta tcaaatctag gaaatatggt atttggtatc agtaaagaat tcagttggac
      241 tactacatta tgggaatctc tattcttttt catcttcgta tttttgttac aaaattatcg
      301 aaaaaaataa acatgtgcca tctatctgtt ggcacatgtt ttaggattta tggtactttt
      361 tattcggcaa tccattttat ttttctactt cttcttaata cctctaacac ttttgattta
      421 taaactaatc caatgcatct acttgcgctt attaagctat ttcagctaga tagtaccacc
      481 ccatgcgtca atgcttgatt ttccttttgt ataacattgt ataagcaata aaggacaaat
      541 aagtggtgat tacaccaaac cattgaataa gaaaaattct gatacgtaaa taaacatgaa
      601 cttgaaaaaa ttaatgaatc ggttagctac tacgtagttg tcttttttct catacagttc
      661 tgttaactta cacttctctt cgctagataa taaaaaatcc tccaactgta ttgtccaata
      721 taatcccagg catctaaact agcattatag aagagacaca catagaatct aaaataactg
      781 gttttaaatg tttcttacaa tcaaaaaaag ctgttaaatg cactccacac cttcatactc
      841 tatcattttt tataaacgat atatttttat tgtttttcga taaaaaatag ttgttttaca
      901 ataattattc tgctatattt tagctatcaa agataaaaga gggataaaaa tgaaatttaa
      961 aacattgcta ttaaaatttg tcgttttagg tataagtgcc gtatttattt tgttcggcgt
     1021 acttttttat actcagatcg ctacaagcga aaaaggattc aattttgatt ggcagaccac
     1081 attatttgtt tctgtcattt actttgccct tctcctaggg atcgttattg cttatttctt
     1141 attgaaacta ttgaaaaaaa tcgaagataa ccaagctttt tccactcaaa gcttaaacat
     1201 actaaaaaaa atcgaacgaa ccattttgtt tattggtttc gccttttttg gcttacttcc
     1261 taaatttatg aaagtagcgg atttagacga tgctccaggt ttgatgttgg taggaatcgc
     1321 tgtcgtattc attccttttg cagtatacac attaacaagc gtgctggaaa aactgcttga
     1381 acatgcgatt ttacttaaga cagatcatga tttgacagtg tgaggtgcga catggcaatt
     1441 atcgttaatt tagatgtagt attagctcaa aaaaaaatga gtgtgactga attgagtgag
     1501 aaagtgggca ttacgatggc aaacatctct attctaaaaa atggaaaagc aaaagccatc
     1561 cgtttttcca ctctcgaaaa aatctgccaa gttttggagt gtcaaccagg tgatttgttg
     1621 acctataaaa atgactcgaa tactatacca gaagaataaa ataaagtaga acaaacagta
     1681 aataaaaaat aatctgaatg atagatgtgt gagactttta gaagttattt acgtctatta
     1741 ttcagatttt ttatttcgat tgaataccgt tttatctata aataagatat gtactgactc
     1801 atacaaaggg ggccttttct tctccatgta ctatcacttt tcctgataca actggtttcc
     1861 tgttacgttg tagttggatc tgcttttagt ataatacttt attcattcca ccagactgat
     1921 agcagtttga gcagtctaaa taatcatgta gtattacagg ccattctgtt tcataccttg
     1981 aagtgcttaa aacttcagct aatggcccat tgtgttaact cttactccat tcttttttag
     2041 taagctccca tatatactgt aaagccttct atatattatg tcatgttgct ttttaacatt
     2101 tactatgtag ttttgataaa atgatttgaa taacagtgat ggacaattct tttttatttt
     2161 taacattatt ttgataataa tgattattta tttaaaaata cattagataa catttgttac
     2221 gacacgaaaa agcttcacaa acaaatatcg ttattttaaa atgagggcga actcacacaa
     2281 ttaggaggaa tagtatgcca tttattgttt acttttttct ttcattactc gtgatctata
     2341 ttccattacc aacatttttc ctgctcgtca atcgtctagc agagcaacat gatacgattg
     2401 ctgctcttac gaaactttgt ttacttctat ttgtttgtct agattacttt acgagctatt
     2461 attggctaaa acagacaaga gtaaaaaaga gatattattt cttccttgtc tttttcaatc
     2521 tctttgagtt tctgatccat tgctttttac ttcatcagta tcatacgatc catttgtgga
     2581 tcatctgggt tttcaaagtg gtcgtattat tttgtatgtt cacctttcct atctcaagaa
     2641 aatttaggga ggcattgttt tattaaagaa gcttctctgt atcaataaag acacatttaa
     2701 ggaaaaactt ttaaaaatct aattgggaat ttgcccgcat aaataagagg ctgtgacaaa
     2761 agtcttgtca cagcctctta ttccgtataa acagtgttca aaagcttgca tcttcgataa
     2821 tatgcccaac caaatgaaaa atatgagaag ctatttttgt ttggctgttc ttattacttg
     2881 atgaagaaca gctttttcac gacctctttt taaaagtctt tttacagttt tcgagcaaag
     2941 aatgatacga ttagtactaa aatgatggca ccaatcaatg atggcacgat tgccattcct
     3001 gcaatacttg gtccccaaga acctaataat gcttgaccaa gtgctgatcc aactaaacct
     3061 gctacgacat ttgcaataat ccccatcgaa gaacctttat tcgtgattgc tcctgcaatt
     3121 gctccgataa ttcctccgac gataagtgac caaataaatc ccatgtttcg ttcctcctaa
     3181 tgttttactt ccttattact gaacccgtgg ttctgtattt tcttttactt tttgtgcgcc
     3241 gcttccagct gctgatttta ctttatctgt ttgattcgaa gcaaattcac cggttttttt
     3301 cgctgcgtca gttactttgt cttgcaccgt ttcttgatct ttttcaaatt cttcttttgt
     3361 tttgatgtcc actacgttag cgttgacttc gatcacttct aaatgtgtca tattttttac
     3421 ttctttgccg attacttctt gcatttcttc aaagattttt tcgatatctt tgccatattc
     3481 cacaatgaca tccatatcaa ctgctacttg ttttttaccg acttctgttt cgatcccagc
     3541 cgttacatta tctgtattca caagttttcc tgccacatta gagaagaaac caccgtttgc
     3601 agataataaa ccgtctactt gttcaattgc gattccgatg attttttgca caactttatc
     3661 ttcataattc agctcaccgc gcattgaatc ttcctgttca gaatcatgct ttccttggtt
     3721 atttgtatga tcagaaccaa gttttccttg tccgtttgtg tggtcagaac taagtttttc
     3781 ttgtgctttc gcatggtcag aaggatgttt ttgctcttcc acaggtttag aaggatcgat
     3841 tgcatcatgt tgttctggaa ctttagattc tggagttcct ggatgatgag gtgcatgctt
     3901 ttctgcatca tgtttcttct cttctactcc ttgttgaacg ttagggacta ctggatctgg
     3961 atgtgcagat actggtgctg ttttgttctt ttcatcgttc acaccatttg attggtttgg
     4021 tgtcgttggg tctggatgtg cagacactgg tttttggttg ttcattttat tgttattatt
     4081 tttgttattg ttattcatgg atgtttcctc ctaagattta aaatagtgat ctaagatttt
     4141 cgtttgtttc agataaaaac cgatagcgac gccgatcgct gccatgatca agatcaataa
     4201 ggtcttgaaa aagcccacgg tgatccataa aacggctaat atcaatccaa gtaccccaaa
     4261 gatgataggg agcttgtatg tcgaaaatag ttctttcatt tttggtcccc ccctattcta
     4321 ctcgtttgcg gtcattgctt cgtttagctg gttcttcaaa gcctgtgtat ttcaccgata
     4381 ctttcactgg ctcttttgtt cccaatatgt gttgtacttc attttcgatt tctttcatca
     4441 atgttcctgt tttaccaatg agtgctgaag ttcgtttcaa ctcccctttt acattgacgg
     4501 cgatcttgtt tttcgttgct ttgacattta cttttggtga atggatgaat tcatcttcat
     4561 gcaagtgaga acgaaccatt ccttcaattg cttttttatc caacgtcaac tccccatcac
     4621 catgtttcag tagaaagttt ccgattgttt ttggataaaa caggaaaaca agtaacgtga
     4681 taattgtcaa aatagcaaat gcaacggcaa gccaaaatac atattgttgc acgtatgaat
     4741 tagtgatgga aaagaatcga aacttctcta gctgaaaagg caatggatag gttgcactta
     4801 atagtatcgc aaacaaaagc acagatagca aaagtaatgt tccgatcact ccgatcgttt
     4861 ttacaccttt attcattttc gcacctcctc ataggggtga aacgatcaac ttcttataag
     4921 ttgatcgctt ctccaccagt tactccgtaa atttgggctg taacataact tgctttattc
     4981 gatgctaaga aaacataaac tggtgctaat tctcctggct gacctgcacg ttctagtaaa
     5041 ctttgctgac caaattcagg gatcgaatca gaaggttgtc cttcgtctag ttgtaatggt
     5101 gtccagattg gaccaggtgc tacaccattt acacggattc ctttaggtgc aagctgtttt
     5161 gctaaaccga tcgtaaagtt agcgattgaa gcttttgttg ctgcgtaatc taataaatgt
     5221 tcgcttggat tgaatgcttg aacagaagtt gttgttacga ttgctcctcc tgctgggata
     5281 tgcggaacag cttctttcac taaagcaaac atagcgatga tgtttatcat aaatgtgtct
     5341 ttgacttgct caagtggtaa ctcagcaata gactgacaag aaatttgctg tcctgcgttt
     5401 agtaccatcg tatctagccc gccaagttca ctcacggctt tatctatgat ttctttaggt
     5461 gcttgttcgt cacgaagatc aaaaggtagt aagacaatct tacgtcctgc atctttcacg
     5521 tattttgcta cttcctctgc atctttttct tctcctggga agaattgaag agcgatatct
     5581 gccccttctc tagcaaatgc aatcgctgct gcacgaccga tccctgaatc tccaccagtg
     5641 attagtacac ggcggttttc tagttgatta ttgccttcat aggattcttc tccacaatca
     5701 ggaacaggaa tcatattctg ctgtaaagct ggtgtatcct gatcttgttt aggaaatttc
     5761 tccgtatggt acaagtttcg cgggtcgatt aattttggtt ctgtcattgc tgtattcctc
     5821 ctagttttta aattgtattg ccaaaacaaa tttctttgtt tgatacctgt tttaaataga
     5881 ttctttaatt tgtataatct ttccggtata aatggtaacc taaaactact gctgctatca
     5941 aagagccaaa gactacgagt ataaagccta gagcagaact tttacccggt aatcctcctg
     6001 tattcattcc ccaaatccct gcgaacaacg ccggaacgga aatgattaat gcagctgaat
     6061 ctagatattt catgatatga tttagattat tgtccatcat cccgttgaat agatcaccaa
     6121 tactctctaa taaatctcga tagatctcga tcattttttc tgtttgtcgc tgtctcaaat
     6181 ggacatcata gagtagctgt tcatcgtcta aacgtttgat gaaagtttca gaattccata
     6241 attgatccat tgtttcccgt tgatctctta aggtatgatc gatatatacg atgtctcttt
     6301 caagatctgc ctgtttatac agttcttcat tttccgttgt tttacgagct gctttatcca
     6361 aagcgtcaat tcgctttttc atctctctta gttctttcac gtaatgggtg taaatatcta
     6421 ataatgtata cgtgatgatc ttttcaaacg agtttaaatg atttccatgc tttgtcagca
     6481 gccgatccac aaattcggtt tttctatcga tacaagtaat caagagatta tcggaaagta
     6541 caaaagatat tggcgttaat ttttttcgat acgattatct gactgatcta gattaatcac
     6601 taccaaagaa aatggatgct ctagtttcgt ctcataaagg cgctccatcc gagaaacttc
     6661 ttccggatat tcggttccta cgaaaatatc atcaggaagc tcgaattctt cgattgcttg
     6721 ctcaatctct tcgggctttg cactatcaag tacgagccaa ttatattttt tttcatctgt
     6781 ttctattaca ttcttttctt catccacaaa gtagtatctc aatgtatacc tcctttttgg
     6841 actttctcga aactattagc tgaagaagca gttctttaat ttgatattat aatcgagtac
     6901 tgcgatttca ttttctaatg aaaagctgtc aagctgtttg ttgaagtcca tgttcaatcg
     6961 tttaccgaaa taattttgct ctaatttaga tgtatagatg tccaataatg gatgctcgat
     7021 tttagtcgca tattctccaa attccgaaac aaaaggaatg atttgaaaaa agctgccttt
     7081 tgcaaaagca cctttgtccc ttagaaatgg atcggaatca ttgataagcg cgagtgcgat
     7141 ttttgtattt tccccaacct tttcaattaa cgtctctact gcatttttag acattgtaat
     7201 ttccattcct actccccctc ttcagttgtt gtcttctatt atttaacaac tacatctgtt
     7261 tttggttgaa cttttgcata ttttggatat ttagggaatt tagctacaca acggaaagcc
     7321 gtcatccctg catatttttc gtaatatttc ttcgttggcc cttcaaaatc cttgctccac
     7381 ggtttcacat gtcctgaaaa atggataatg taaggattgt tgcgagtttc ttcgtattca
     7441 cgttcacctg ctgctgttgg atgttttttt gcttttgcca tgatatagcc ttgtgcattc
     7501 caacgcggat gaagtggtaa ccaacggtca tgtaagatag catttaatgc atcttggtca
     7561 tggaatcgta atttttctgg attatgttcg atgaaatcta atactttttg cgtaatattt
     7621 tcatctaacc attttttcac attgatcagc atcaaacctg agttgaaata acgcattgat
     7681 ttagctgaaa ttttcatttt ttccagtcgt tgatggaacc cagcgtcttc aacagctgct
     7741 acgattgaat caccgaagtc aagacgccat aatttagaga tatcttgtaa agcgatcatg
     7801 tcgcaatcca tatataagat acgttcaact tctgtgccgc ggaacaattc cggaatcgca
     7861 atacgataat aagctgtttc tggaatacga tcgctgatta ccacatttgt aaagaattct
     7921 ttattgattt tcaagaattc cacatctgag ttcattcgtg cgtttttcac tgagaagcgt
     7981 aaaagctgtt tactttcaaa atcgatatca tcgtcaatca catagaaccg aacaaattta
     8041 gacgggttgc aattatctaa tactgagaca aataatgcag ctaaatgtgg tacgaatttt
     8101 gtgttacaac ttgcgactac tgctatttct tttttattca ttgatctcgc ctactttcgt
     8161 tagagtactg tgtgctaatt tttttaagta taaattggta tacggatgat ctttcaacgt
     8221 attccaaggt ttatcatgtc ctgtaaagtg aacgatcgaa ggtttttcat ttccttcttt
     8281 atactgacgt tcatattttt catttggtgc tggatgacgt tcaaaaatca gcgaagtctg
     8341 catgttccat tttggatgaa gttgttccca atcttcataa agtacagcat tcaaagcatc
     8401 ttggtcgtga tagatgattc gatcgccatt ttcactgagg taatggatag ttttttctgt
     8461 aatttctttt ttattccatt gatcaatgtc aatcaccatc actcctgaat tgaagtacaa
     8521 gtcatcggaa tcgattccta atctttccaa agctttcact tggcctggat cgataatagc
     8581 gccgattgtt tttccattta acggctcatc atatagtttt acgatatcgt ctagtactaa
     8641 cacatctgaa tctaagtaaa ggactttctt ataatcctct ttagctaata agtttggtaa
     8701 agaaattctt aagtaagcgg tagttgtgat atggtcactg actaggaagt cttcgtagat
     8761 atctttttcc actgtcagga attggatact tgcattactt gaatatttat ttaccgtttc
     8821 ttccaatcct tctttactat attctgatag accatcgtca atcacataaa attttatgcg
     8881 tcttgtttta ttgcaatttt ctaatgcagt tgcgatcatc acgctcaagt aaggagcata
     8941 attttcgtct gaagcagtta ccacaggaac tacgccgtat ctcttctcca tcaaaatcac
     9001 tcctttttct aatctttttc taacctgttc tacatctaga atattcctca tttggtttta
     9061 aagcaaaata tatgctttgt tttcattgaa tattttaaaa tttttttatc acaaaacgca
     9121 tcggtaagcg ctaactattc gtttttcatg gctttttatt caaaggtatt gaccaaaaac
     9181 tactatatat agccaccttt ttcttaagaa tgttttttga aaaaattcat aatgacaaag
     9241 caagttagtt tcattttttt aaattctcat ttaatcttaa ggtaacagga aaatagatat
     9301 gtaaaaagaa agtaggaaga taaaatgaaa ctagcagcga ttgatttaga cggtaccttg
     9361 cttgatcgaa atggaaagat accagaagaa aatatccaag cacttcggaa atttgatgaa
     9421 aatggcggaa tcgtaaccgt cgctaccgga aggaacagca tttctgctaa agatattttt
     9481 tccgaattag gaatcggtgg ctatttgatt tcttccaatg gttcactgat tgctgaaatg
     9541 aaggatggaa aaatggcgaa agttctcaga cgttcaaaaa tagatagcga tacactgaga
     9601 aaagccttct tctatgctca agaagcgaaa atatccatca ttgctagccg agaaacccaa
     9661 gatgatcagc tgacattcga tgaatcaaat ctagtcaaag atgaccctta ttatgaacat
     9721 ttcaatttac agaaccattc ttttgatcaa attttaaata tgttggaaga tccttctctt
     9781 agctatctta aattagcatt aacagataag agtgaagaaa aactacagaa gctacaaaga
     9841 gatctgcaag cagatggtat ccaatcagta ttctctgatc cgcatttctt agaaatcacg
     9901 cctacaggta tcactaaagc tcattcactt gtttttctgg cggattacct tggaatcgaa
     9961 tcgagtgaca tcatggcttt tggcgatcaa gaaaacgaca ttgctatgtt gaattacagc
    10021 gggttgagta ttgcgatggg gaatgcacag cgacaagtaa aagaaattgc aaaccaagta
    10081 acactgacga atgaagaagc tggtgttgct tatattttaa aaaacttcta ttaaaaagaa
    10141 taaaatgacg atatgaatgt aggaggaaag aaaatgttag ttattattga tatgcaaaat
    10201 catattcttg atccaaacag cgaattttat attccagagt cagatcaatt ggcagaacgt
    10261 attgctcaaa gattagctaa agcacgccaa aataatgaat atattctctt cacacgggat
    10321 ataccagttg aactgaagga caaagaagaa gaacgagaag atttgcaatt gatccctctt
    10381 ctttctccac aagaaaatga acgagtgatt aaaaagtatt actttacgat tccacctgaa
    10441 actttagctg aaatccagga aactttattt gaaagcaaag aagaacaaaa agagatcgaa
    10501 gtagtcggga ttgaaacaaa cttgtgtgta ctctctaatc tgataggcct gcagagtgct
    10561 tttcctgaag cagacttttt tgttgatcca atgttagtct ccagtagaaa acacggagaa
    10621 tccgctttag agctgttgaa agatttcaac gtctctatcg tcgaatcata aaaatatcta
    10681 tcttcattta cgatatttaa cacacaataa aaaaagcacg cagaactgtt ttagtcctac
    10741 gtgctttttc tatcaaagta gattggcttt tttatgacac agagtgaaac ggcaggctca
    10801 cgacctcttt cttaaatggt gttcaatcag ctgaccctcg gtattagctt aacaaacggc
    10861 aaaatatgag gaactatttt tccgtttgtt aagctaatac tcaggtcaaa acgctgattt
    10921 cacaacctct tccttaaacg gtattcaaaa gcttgcatct gcagtaataa gaacagccaa
    10981 gtaaaaacat aagaagctgt ttttacttgg ctgttcttat tacttgatgc agggcagctt
    11041 tttcacaacc tcttatgtac cttgtaggac tcgaacctac gaccggacgg ttatgagccg
    11101 tctgctctaa ccaactgagc taaaggtaca tcataaataa aatttatttt tgttactttt
    11161 caactatagc acgaatgaca aactctcaca agtttcaaaa tataagtaaa ataatattgt
    11221 tattcttatt tcttttactt gtgtcataac tattttgtta ctttaatttt tatttgctta
    11281 ttttagaaaa aatgttattc ttttatttat tctcaatgtt aggagtgatt attttgccat
    11341 ttattgcgat tttattcgat ttagttgcgg cagcagctta ttttctccaa ttaaattatc
    11401 aaacctcagg aattctgttt attggaatga tttttcaagg tgtgattacc cttttgcttc
    11461 ttgtcatgat gctcagctat aaaggaaaaa gatatgcccg tattcaaaca aaagtatttg
    11521 ttaaatatgt ttccattcgc tatgggatca tcattctttc ctttttagtg aatgcgatcg
    11581 tcctcttttt atatgtattg aattacttag atatcaatcc attgattttt tctcgataga
    11641 taagacacat tcagcggaac agttcctgct tatcagcaga aaactgttct gctttttcat
    11701 ttttcattga tctattcttc ttttattttc ctagcatttc atttgtgatg tatgcaataa
    11761 catgttatct tttattatat gaatcgttga ggagtccgtt acatgattac gttggtgaga
    11821 gttttgtttt gggtgcctgc ggtcgcactg gtcgcaagta ttgtttatct tatgaattgg
    11881 aacaaagaac ggttttattt agctattttg actcttccgg ctatctactt tatgtggaaa
    11941 gtttttaatt acaactactt tgaacccgat agtgttttta tagaagaatt atcaggactg
    12001 gttttgtctt tattgatcgt cattctttat ttgatacgtt taaataaaaa acattgagta
    12061 ctattgattg catgtgtagc tttctatagt cattataact attctactat aatgaaaggt
    12121 gattaaatga gtatcgaaga agctatttta ctggatactt tacgttgtcg tctgaatttc
    12181 ttaagaactg gccgatcttt atctcaagta gctgtttcta gcgcgttaag tattccttta
    12241 ttcaaatatc gtaagtacga agaaggagaa gaaattccaa gcatcattga attaatctat
    12301 atcgctgact tttacgatat ttccgttgat tacctaattg gtcgctcaga aacagcaagc
    12361 aaactgaagt gattttttcc agccttggtg ctggtttttt catttaacgt ctaaggttaa
    12421 caatctatat ataaaggaga catttctatg cgattatggc atgaaaaatt gatttcaaag
    12481 cttcctcgtc aacagctttt aggacaacat cgggaatgct gtgcattaag aggaaatggc
    12541 tggggaaaga aacatgctac tgtggattat gtttttcact attctcctta tagattattc
    12601 caatatcatc agcttgtcat ggatgaaatg gaaaaacgtg gttacagccc agcaccagag
    12661 tggaaaaatc ctatgtatcg tggcaaatca tgcgaagcat tttttgattt acctactgta
    12721 gagcgcaacg tgccgattta tccggaacac gatgaagttt atctagcaga atgtttagca
    12781 aatttggagc aaaaaggaat ttatcttacc taatataact agaaaagcct tctatctcct
    12841 cttacttcat taaaaagctt taaccatctt agatcaagct aaaatgtcgt aagttttagg
    12901 gttatcttca tcctcacgtg caaaactaaa agcgtactct agttttggtt catcgccaac
    12961 ttccctagac ataaatgaac atctcctgac gtggtttaaa agtaccgcat cttcgacgtc
    13021 tgtatacgat gcacagcaca gattgcttct tttgatcttt cttttgattt ttctgttgct
    13081 tcttctacac cttcttcgat tactacatcg atgcttacaa ttgcttctgc ctaatattcc
    13141 catgcatcgt cgatttaagc ctctgtagtc actgtgatcg tttccacatt ttaactcctc
    13201 tgtcgtggct tcagttgatt ttgtgatcag ttcagttgct aagccagagt cagaacaacc
    13261 tgccattgct agcgttaata ttgatagtaa aaatagtttt tttatcataa ggttcccccc
    13321 ctgaaaatag actaaaataa taacgctttc tttttttgtt ttcagacgaa aattaataat
    13381 ttctaaatat tctttaaaaa agcttataac tattatgcct tttttaatat ataacacagt
    13441 tatttttaaa aataagaatg ctaaatcact ctacaataac ggcgaaagca caaaaaataa
    13501 aaaaattatt acgttattaa aaaaacagat gaaagatcag gttttattag atctcattct
    13561 ttcatcactt gaagggattt aatgaactac tttttcctct ttgtcgtttt tatatattcg
    13621 tattccttca aacagtagga tagcctcttt caatgcttga tatttgaact gtccgttact
    13681 taaaaattta gatgtgccgc cctcttccta gaacatattc ttttttactt tttcattaat
    13741 tgacagacca atcgaacgaa actgtaaatt tcgataggat aatgattggt caaactcatt
    13801 cattttcaag acatctcgat tcatattcaa gataggaatc tttttatcaa catcacttta
    13861 ttcgtatttt cctctaccat aaaagcttca cgctccacaa aattgtttta tacactacta
    13921 ttcagcccat tcaacgacca atgaattttt tcgtacaaaa aaaccgtctt ttctttcgaa
    13981 aagacggtta aaagtcaatt tttattaagc tttaacaatg tttgttgctt gagggccacg
    14041 ttgaccatct tcaacatcgt aagacactgc ttggccttct tctaaagttt tgaagccgtc
    14101 accttggata gctgaaaaat gtgcaaatac atcatttcca tcttcaccag tgataaagcc
    14161 aaaacctttg tctgcgttga accattttac tgtaccgtta ttcataaata aattcctcct
    14221 gatacgtatc aatgtacgtt ttttcgttgc aataaatatt tgataggcaa aaggagtttt
    14281 atctatgtag atatatcgct caaattacgt ttcaaaatag attactcatt taatatatca
    14341 tgctttttct aataacacaa ggataatgca atgaaatata aaattcattg tcttctattg
    14401 tcttcgaatc gaagaaaaag ttgaataacc gcgattgatt cactttttgt tttcttgttt
    14461 atttgctttt atctgcaact tttgacttta aaatttctta actatgaaaa cctttcaatt
    14521 ggtggtatac ttataaatgg aatttcgtta tcaacgcgtt cgttgccttt cagcaagtat
    14581 ttaaagaaag gagaatggct atgacattct ataacaaaga tatacaatat cgagtaacct
    14641 tggatacaga tttgaatcta tttgtcgttt ttgacaaaga aaataccaat catgtagcga
    14701 caggtgttac gatcgaacaa gcagtgcaag aattaaaaaa atctgcataa ggagtggaaa
    14761 ccctcatatg gacgattctc gttcttctct ttaaacgcat ctactggtag ataacagtag
    14821 atgcgttttg tgtttgttct aaattgttca ctacaaataa ggtagatact tcaaaataaa
    14881 aaattacttc tttttcatct gttccctcag tcgtgcagga caataaataa agaggactag
    14941 aaaatagtcc tctttattag agatacaacc ctttactttc taaaaatcgt tttgcttgat
    15001 gcaggtcata aaattcatag tctgctttat tggattcata taaacttaca tctactttca
    15061 acttatgctt cccattatga tcgggcaacc ctatcaaatg atctttgatg agaaaagaca
    15121 aaatgcccgc tcgccttgct gcttcgatcc ctgcaatcga gtcttcaaat acaagtgctt
    15181 cacttggagg aatattcatt tttgaacaag ctttcaaaaa gatctctggt tccggctttc
    15241 ctttttttac atcatcccct gaaatgattt gatcaaacaa ggcatataga ttttccattt
    15301 ttaaatacat ttctactttt acccgtgtat tagacgaagc cacgattgta tggattcctc
    15361 tttcttttgc aaaagataaa atttcttctg ctcctttttt cgtatatact ctcttatctt
    15421 ctttcacgat tttttcttta tattgattga ttgcttctcg ccatttgata tatggaacat
    15481 cttctccgta caaaatgccc atttgttctc ttatcgccat ttctgtttgg ccagttagta
    15541 aataatagac ttccttacgc atttcataat catacttttg tgctgatttt acataagcac
    15601 gataagctaa acgtccagtt tctaataaaa ggccatccat atcaaagatg aagagcttga
    15661 ttttattcat aatgctctcc cagtggtttt tccttactta tcgcctcata tagaccttgc
    15721 atgtaagtga gtcccattgc acgatcatac aggccatatc caggcatagc cacttcaccc
    15781 cacacggttc taccatgatc agggcgtata ggaccgtcga acccttcttg caccaacgct
    15841 ttcataatat gatacatatc aagggagcct tctgtagagg gatgtgccgt ttctttgaac
    15901 tttctttctc ctaaaaactc cacgtttctg aagtggacaa aatggatgcg cgaacctacc
    15961 tcatggatga tttcaactaa atcgttttcc ggatttgccc ctaaagatcc tgtgcacagc
    16021 gttagtccat tatatggaga atctactgca tttagaattt tcttcaagtc gccaagattt
    16081 ttcgtcactc taggaaaacc aaaaagttcc catggcggat catctggatg gatagccatt
    16141 tttacatctg ctttttcgca cactggaatc actttttcta aaaaataagt cagattttca
    16201 aataattctt cttcggtcat tccttcatac agtttttcca gctgagcgaa ttttttcaac
    16261 cgttcttctt cccaacccgg caatttgaat ccctttgact ggctgtgtat caattgatac
    16321 atttctcctg gctccatctg atcgacgatt ttctgatcgt ataaaagaga atagcttcca
    16381 tcgttgtttt cattaaacaa atcagttttc gcccatccaa aaattggttt gaagctataa
    16441 caaatcaatc gaaccccaca tcgagacaag tttttgattg ttcgtatata gttatcaata
    16501 tagtaatctc gttcatctgt ccctgccttg attgcatcgt ggatcgctac actttcaatt
    16561 ccaagcagtt ccagtccttg gctctcaaca gatgctttca actcctcaat ttctgaaatc
    16621 tcccaaacat ctccaggcat tttagtcagc aaagttccaa ttatcccatt tattcccggt
    16681 atttggcgaa catattttaa tgggatcgaa tccccttttt caccatacca tctaaatccc
    16741 cacttcatct tttcagcctt ccttctaaaa atcgtcctca tctaattttt tttcttccgc
    16801 tgaagcatgc caaacagtta aattatcttt tccttgtttg acaatttgtt ccaccgcttc
    16861 ttctatcggt gtgtgaagca tctgatgatt gattgcttct aacaacattg gcagattcac
    16921 gccccctatg acataagtgg ctgctttctt ttcttcagtc agttcattga ctgccaagag
    16981 cgcttgattg tacggacttg cgctaattag atcaacaaat actagcatgg gttctgctgt
    17041 gttgatcata gttaattttt ctttgacctt ctctccgaat gtttggatgt cctccccaga
    17101 ctctaagcac attgtttcaa tatggctcga tgtcccgaca attacttccg ccgcatcttt
    17161 cagtccttcg ctcaacttac catgtgtaac gatcatgatt tccaacatta gtcttctcac
    17221 tccttgtttt gcacttttta cttatcgaaa aagagcaata atctgattgg cattttgcca
    17281 attaaatgtt ttccctcgta aatctgtttt ggtcaaatag aagaactgcg cttgcttttt
    17341 tcttgcacta aaaatataat tgacgttttt ttctttcaac ttatcaactt gtttcagcca
    17401 ctgttcttta tttgaaaaat aatttcccga aaactgtagc ggtgtatctg ccccaacaga
    17461 gtctagataa aaaagaatag cctctttttt catacccgat ctcacgctgt ctaagccctt
    17521 ttctccataa cagccttcat caacaaacgc atacgctaca ctcggatcat caatcgtacg
    17581 gatcatttct agcatcgtta atatggatga agtatttcga ataaaagttt gtctgctagg
    17641 ccatccgcga gctgcttttc ccaataagaa aaagtagctc ccaaatgcta gtacagtgat
    17701 acctgtttat acggatagca gatcgaatac gtgatgagga tttttgttat atatcatcat
    17761 tccccaccaa cctaaactga acaacaaaat gaaggacaat aatatagagt agattgtttt
    17821 tttcctttgg tctttccaat caaacatgaa ataacttcct aatgcatgaa cgggtgtatc
    17881 gtaataagta cagataactt tctctgcttt ttcgatatct ccaacataaa tattttttga
    17941 tcggtaggct agcgtatcgt aagcaatgac tgtcacatct gttctcatac tgtaaatatc
    18001 tgatactaat gctgaaagaa aacgctgttt actctttttt gtacggcgct tacggtaaat
    18061 atagcgataa cgaaattccc aatcacgaaa cagctcgttc ttcttcattt tttcgccctc
    18121 tttatctttt ttctaagcat ttgtttatga caaatcaaac ttgttcaata catcttttaa
    18181 tcctgtttta ggtgcagatg gagtggcttg aaaataaata ttctttacgc catattgttc
    18241 attcatttct ttcaattttt cagcatctga tttatttaaa taaacagatt tcgtcactaa
    18301 aagatctgta tcaggatttt tttgagcaat ccctccaata ttcaattctt ccatggggat
    18361 tccgtgtttt actaagtcgt aaatcgtttg gactgttttt gcgatcaaaa tacaattgta
    18421 ttctttaaat tgatattcat cccaatagaa tttagctttc tctggtgtaa tgacaattgt
    18481 tttttgtcct gtacggcttc ctgcattttt gtaaagatct ctcataaatt tatcttttgc
    18541 tacaacttcg tctacaacaa aaatggaatt cactccactt ttttgagata acgttaccat
    18601 tacttgccca tggatcaaac gctcatccac acgcgctaaa ttgactacac ccatcttatt
    18661 cacgttccct tctttttata aaataccgat tcctgctagc acaattaata ttccagtcag
    18721 ccataataat gcttgtgtga ctttcaatcc ttttttcgta tagaacaagt acacacccat
    18781 tacgacagct aaaggtaata tccctggcac gatttgatca agaatttcct ggataacaaa
    18841 ctcacgtccg gatatcgcaa atttcaatga agattctact tttacatagt ttcccgcaag
    18901 cacccccatc ataaacaacc ctaaaactga tagcatttca atcgctgtct gaacaccagt
    18961 gttttgcttt aggcctgtag cattacggcc catagtgaaa gcttgtcttg cgaaaaatac
    19021 ccctagtaaa acctgataca acccaaagca gataaaagga aaaagtgcgc ctaaaggact
    19081 tccctgttgt gcccaaggta ccgcgattgc gataaaaata tattggacgg ttcctgaatc
    19141 gattgcatct ccgattcctg ctagtgcccc catcaatccc gctttcgtgt tatacatcaa
    19201 gtcatcctgc atcaagatct ctttttcttc atactcttct tctgctcttt gctgttccat
    19261 agaagacatt aggccagtaa tcacaccgcc accccagctt aattgagtgt tgaagaacag
    19321 taactgccgt ttatatgctg cttttaaaga gtcttcatct ttataaagtt ttttcaagat
    19381 tggcatcatt ccgtatagta gtgatggcgc caagtaacgt tcaaaagagt gaggaatttc
    19441 gtttgcaaaa tgccatctca gataggcttt tgttacatct tttttcgtga tttcttcagg
    19501 agcacctgtt tgattatcca ttatttcagc catttcccat tcctcctaaa ttaaaatccg
    19561 tcatcatagt catcttcgtc atcaaaagat gctgaattgt tattgcttgt ggcaggctgt
    19621 acttgtttcc ctttttgcga ctggacaaag atcaaagcga taataatccc aaaaatggca
    19681 taagtaacca tcgtaatatc taaagatttc aaaacaacac tcataaaata agcaaggaag
    19741 aaaaagacca gataatcttt tcgaccgata acgtatacag ttgtcgcgat accgattgcc
    19801 gctaaccctc cacctactac ttcaagaatg tggataacga ctgttccctc taaagctttg
    19861 attacatcag cgatcaatgg cgcccctaaa tataaagcaa tgaaaacaag cggaacgaat
    19921 aataaaaatg aggcaaccgt tggataaaga ataattgatc gggccatagc ttttgtattc
    19981 aaatcttcta cagctttgtc tgtatattta cctaaaaaag tattgatgaa aaaacgaaac
    20041 tgatacaagt aacttccgag caatccgact ggcaccgcaa cggcaatcgc tgcttctggc
    20101 gacagatccc ctaataatgc gactggtact gcgatagctg ctgcaataga tggttcagct
    20161 ggcattgatc ctcctggaga aaaaaccccc atataaatca tttgcaaagc ggctgtaaca
    20221 atcatcgctt gtgcaacttc ccccatgatc aacccgacga ctaatccagt catcaaagga
    20281 gaaaaacgta aggtcaacgt ggctcctcca cctaaccagc gggacataac cgcggctacc
    20341 cataaagcaa tcaacaaact ttgtactaca cttaatgttt ccatgtgtcc tcctccttca
    20401 gatctttctg atataacctt ccagttccat cattgactgt atcagtgatt tctgtgttac
    20461 ctctatagga atcaaatgca ctttctcttt cgaatcaata aattcagcca ttttatctat
    20521 tatctgttta tctttcggaa ataatcccat ttgttctaga gaaaccggca gttctaattc
    20581 ccgatacaaa ggatataact cgtctatttt ttcccagcga ccttctagtg ccagttgata
    20641 gaaaatccca tacgctactt tttctccatg tagaaaacga tggctttccg gaatatattt
    20701 actgatagcg tcatgcatag catgtgctgc cacatttctt gcgtatttat ctcctaatcc
    20761 tccaactaaa ccagcaacag caaagataat ctctgaaagc tgtttgaact ccgatgtcag
    20821 ttttctttgt gccatatctt ggattgcttt agcagaatga ttcaaaatct cttctttgca
    20881 gatcttcgca gtatcttgtg ctagcttcaa aaatgattca tcttgcaaaa aatcttgttc
    20941 taagatgagc tcagattcat accattttgc tagtgtatct gctaacccgg caatgaaata
    21001 tggaacaggg gaatcaatca ctaattgggg atctgttata aaaaaagcag cttgtctgta
    21061 aaagtgttct gttttccctt ctgcttgtcc attctcctta tacatgacag caagaggtgt
    21121 ccatggagca caattactag ctaaagtagg gatgactccg aaatcgattc ttgcaagatt
    21181 ggcactataa cccactagat cggtcagttt tcctccgcca acgccaacta cgaaatcgat
    21241 ttcatttttt tgatgattgt agctattcgt ctggcttcag cataactaca ttctccagaa
    21301 taacgatgat aaaaaaattg aatcgtttcg tcattcaaaa atactaattt aggtagcgcc
    21361 ttttcccatg ataccgttcc gtgggtgacc agtactcttt tcgcgccgta tgatttcaag
    21421 agatcagaaa cttttgaaat accaccttct tcatataaat aaaattgagg gcctgttttc
    21481 acttttaaac cgttcaccat tttcttcatc atccttttat atagttgctc tttgtctgga
    21541 cagaacgttc aggaagaagg tgctgactga tctgttcgac atctctgaca catttttcac
    21601 ccattgcttc taaacattcc cttgtgtagg ccgatatatg tggcgtaatc actacttgtt
    21661 caaacgctaa atacggatga tcttgacgtc ccggttcttc ttctaaaaca tctgtggcat
    21721 atcctgcgat ctttccgctt ttcagaccat taatcatcgc ttcttcatca actagagcgc
    21781 ctcttgcact attagataga taaacatttt ctttcatctg atcgacttct ttatcagaaa
    21841 tcatgtgata gttctcttct gtcaaattcg cacataaaca aatgatatct gattgttcca
    21901 gtaattcttg aagttctact ttttttgcac caaagctttc gatgtataaa tctgatttgt
    21961 aaggatcata agctagaaca tcacagcgaa aaccgttacg caaaatttcg attactccgc
    22021 ttccggtatt tcctgcacca atcacaccaa ctgttttgtt aaacagcgtc ctaccgacga
    22081 actccgcccg atcttcccat cgatcgtcct tgactcttgc ttgtgcttgt gtcgtgcgtc
    22141 gaagcatgga caacaaatta gttatattat tttctgctac agcatttcgt tcaactaaag
    22201 caggtatgat agatacgatt gtctggtgtt cttttgctgc ttttaaatca atattgttgt
    22261 aaccaatacc atgtcgggaa atcaatagca gttcatcttt gttttcaaaa aaatctcgtc
    22321 gaaaaaatgg cgtaacactt gcaataatga tattgaatcc tttaagtaac tccgctagtt
    22381 cttttcctgg aatctcttga tcaacagtaa agaaatgaac gtctccaact gactttaatc
    22441 tttccaagtg gtgtggaaag actcttccga aactgctgga attaacgatt gctatatgat
    22501 aatctggcat tttactcacc cttttcaact atttcatgtc cgccaaaccc atttcttagt
    22561 gctgaaacta cttttgctga aaaactatct tcttcctgtg aaagattcct catgaacaaa
    22621 ctagttgcaa ttactggaac tggaacagcc atttctagcg ctgtttcaac cgtccatttt
    22681 gcttcacctg atgcagaaac gattccccga taattactta aattgggatg ttcgtttaac
    22741 tgatcttcca tgatttccat cagccagccg cgaatcaccg aaccatgatt ccaaacacgt
    22801 gcgacttgtt ccaagtcaaa agcatactca cttttttgta caagttggaa tccttcaccg
    22861 attgcttgca tcattccata ttcgattcca ttatggatca ttttcaagaa gtgtccgctt
    22921 ccggatgcac cagcatacag atatcctctt tcaattgcta catctttgaa taattgttcc
    22981 acggatagaa aagtttcttt atctcctcca atcattaagc aggcttgatg tcgtgctcct
    23041 tcgattcctc cagaagtccc acaatccaag taaccgattt catttttctg aaactcttct
    23101 gctcgacgga ttgaatcttt ataataagag tttccgcctt caataataat gtctcctggt
    23161 ccgagttttg gaaaaagttt tcttaacatg ttttcggtta tctctccagc aggaagcatt
    23221 aaccagatga tttttttctg ctcaaaagta gctagaaatt cagagatctc ttgataagca
    23281 ggaatgtcat tttctcttgc ctgactaaca gctgtacttg aaatgtcgag tccttgtact
    23341 ttgtgatggt gctcttttaa gtttaaagct aaattcaatc ccatttttcc caaaccaatt
    23401 acaccaattt ccatgatagc gcctccattt cttataccct cattatacaa aaaaattttt
    23461 ttgtaaatag attttacaaa aatatttttt gtttgatttt ttcctactct ccttgtatct
    23521 ttaacattaa tgagaacgga aaaaatgata atcttattat gaaaatgagg aattctcatg
    23581 agcgtctcca taagactaca aatcaaaaca atgtacaacg acttcagccc aaaagaacag
    23641 gctatcgccg attatatttt agaaaatcca agcaaagtaa gtcatagtcc tattagcaat
    23701 ttagcaagtg aactgggcat tgctgactct actttttttc aatttacaaa aaaacttggt
    23761 tataatggat ttaaagattt taaaatggcc atgttgatcc aagaaaatga tctatctgct
    23821 atttctatcc acgaaaatat tcagaaaaca gatactgagc tcactatggc gcaaaaggtg
    23881 ttcgagtcta gtatttcaac tttaaatgac acaaaaaagt tgttacaaca aaaggatcta
    23941 aaaaaagcgg ctactattat caatgaatct aaacgtgtct attttttcgg tgttggcggt
    24001 tcagagattg tagcaactga cgcttatcat aaatttttgc gttcccctat cgcaacttct
    24061 cacagcacgg attaccatat tcaattgatg gaagcttctt tattgacgga aaaagactgt
    24121 gctgttttga tttctcacac agggcaatca aaagaaacaa tccatattgc tgaaaccgta
    24181 agaaaaactg gagcaaaaac aatcgttatt actagccaag ctaattcttc ccttgctaaa
    24241 cttggcgatg tcgtttttat ttctatttct gaagaaacag aattccgatc tgaagcttta
    24301 gcttcccgta tttctcaact aagcattttg gactctctat atgtgatttt gatgttttac
    24361 aacgaaaaag aatcacgtcg ttctatcgct aaagttcgac atactatttc tgaaatcaaa
    24421 gaatagcacc ataacagaaa aagcgaaaaa tccataaaac aaaaaaagcc actaccaaat
    24481 atttggtagt ggcttttttg gttctgcaag ggtggtacag aacatcaata gtcggtgggt
    24541 tagaacgtat gtcatcgcat ctttggagaa ggttgtattg gtattattcg agttacttgt
    24601 tagatgcgat aacaaagtta gtatacagta gtaaaaaatt tatcttttct tttttttatt
    24661 atttttgttt cttattttct aaaaaattaa aaaaaatttt ggtgaattaa gtatttcctt
    24721 gataacactg gcttttggcc tcattttagg ttatcatttg taattttatt tcacagttga
    24781 tatgattgtt atgtaaaata aaaaagttta ggatacatcg aatttttggg gaaattcttt
    24841 tgggggtatc ctaaatcttt tctattatat tcccatattt tctattattg gtctagacgt
    24901 atgatggtaa actttgtacc tatctgattg ctgaatcatt aattgcttat cattagccat
    24961 ctttgtttca agttcgctag atgatcattt tattcacatc ctaacaaact tgtcacattt
    25021 atagattctt aatctattta tcatacaatt ataaaaaagg aggtgctgct tatgaaagct
    25081 atccatattt ctttgctttt tttcgctagt gcattgattt tatttgctat tcatctttgt
    25141 tcccagcctt atatcaataa caacggaata ttagtggaac cagcattctt ttgcttacca
    25201 ctcggctttt tgtcccttct tgcctccgca ggcttcggat ttgttgcttt ttttaaacga
    25261 tcaaaacaac aaatctgact tataaaattt acgtaaaaga gatcgtgaca ataatcgtgt
    25321 gatattccca aatttcgaat aaaaggtgcc ccttgccatc tctaactcct cagtcacaag
    25381 ttacaatatt cataaaccat gaaaagcttg tttattgaaa ttgctccttg tgacttggag
    25441 ctaagtgaca gattacaacc tcttttgcat cataagactt tactccttga aaaacattac
    25501 taacttttga tatgtttctt gcgcttgttt attgctgaaa tcaaactgat attcatggtt
    25561 caaaacagca tttttctccg cataatctac ttttgtgact ttcacccccg ctttctcaaa
    25621 tacagaagtt acttcttcca tttgtttcgt aaacgtattt gtatttccat ccgttaagaa
    25681 tgtgttttgc gtccaacgat ccgcatgtct gaccaaggct aaacttttga ccttctctga
    25741 ttgttcaaag tccttattat caaagtaagc ccatccacat ttttcataaa gaaagctaga
    25801 gattttatca cttacttgag taagtgcaga aggatccaac agaccagaca aagaaataaa
    25861 cttcgtaatc tgcttgttct ccaaagtcgg atcaagatga tcagcttttt taatttcttc
    25921 attttgaagt gctaacaaat attctccact aatttgtgca cctgccgaat ttccaccaat
    25981 cgaaatattc gaccaatctg cttgatcttt tatctctttt ttagctgctt gaatggcttc
    26041 gttcaattgg ataactggtg taggatgttt tgcttccggt gatcgttcat aatctatatt
    26101 tacgatgatt tgctgcgtgt ctgaacatag atattccaaa taatcacgac aatcattttt
    26161 atcccctcct acatatgcgc ctccatgcat ccagaaaaga atcggtaatt ttttcttttc
    26221 cactttcggt gtgtagatat ccatattatt atttttgtac tttgaaggat aggatatttc
    26281 tttttttata tcgatttgtt cgattttttg ctggaaatca tctggtcggg catatacttg
    26341 ttctttttca cttgtgtcaa attgttttct aacaagcaag ctaatcggct ttggtgtgat
    26401 ttgattaatc aaaaatacag tgactaaaat aactacgatg ataactggta ctgcaatcac
    26461 tattcgcttg aaccatttca ttcatttacc ctctctttct tcgccaaatc catgtgataa
    26521 tcgtgctaac agataaaaat ccacctgaat aaatgagaag cgaatgtttc ttttctccag
    26581 ttttcggcaa cattcttttt tttgcatctg tctttttttg tactttcact gtcatctgaa
    26641 tcggttgcgt atcatcactt gattgatcct ctcgaggttc attctttccc ttattgtctg
    26701 cattctctgt cggatcttgc ttttttggtg gagctactgg gatttttttg taatagaaaa
    26761 taatagttgg tatcgtctca ttaaaaatcc cttcagtcgg tccttcaaca tttgctaatt
    26821 cgtatccttc gaatgtcttt tgctctgtca tataattttc tccataagct ccgtaaagct
    26881 ggatttcgtc ctggtctttg agtagacttt tattgttttc atccacatac tttacgttta
    26941 tctgatcatg gaacactact ttcaaaaagt ccgttacatg tttcgtgtag gcagcatggt
    27001 caacttcctg tgcctttcca tgccctgctc cttctactaa taaaagatct ttataccctc
    27061 ctgataactc atataatttt tgcgcgttag acacaggtac tacatcatct gctgttccat
    27121 ggataatcaa tttaggcaat gaatctgaca ttccaatcgt aggtagagca gcttttgtat
    27181 ccaaatgcat ctcctgatca aaatagtatt gtttcaaaag gcccataaat aaatcttcat
    27241 gctcttgatc cccaatgatg tctaactgat tttttatggc atccggtagt gcgggggcta
    27301 ctgtctggct gtacgtaaaa cgtagttgtt catcaattga tgcaaatccg caattttcaa
    27361 taattccttt gacattgaca ggaatatctt tcgctaatac agacatcatc gtagctgctc
    27421 ccatcgaacc accataaagt aaaatctttt gagatggccg ctctcgaact tcttgattta
    27481 tccagtaaag gacgtcatct gactcatatt gtccataggt cacataatcg cctccgctat
    27541 taccagtcgc tctggaatca acaaataata aattataccc ggccttataa aaatcattgt
    27601 attcttctgt aacgatcccg ttattccaat tccctcgaaa tccgccatgg atcatgactg
    27661 ttttatctga accttgatcg acataatatg catatacatc catcattttg ccatcttgtt
    27721 tgtttagtac agtttgatga agttgttttg tatttggata gaagcctgtg ttttctgtag
    27781 gcgttgtccc atcatctgta cctccaagca tcgacatcaa cacactgttt tttgcaaaag
    27841 cgatatcgta caatagataa gtagcaatca gatgtttgat aatctcttta tctacaccat
    27901 aatcattaac agcaagctca gtgactaata actctattaa ttttgacttg atgtcttctg
    27961 ttggtccatt aagtatttct gtcatctgtt taccgaagta attcaaatat tctttaccat
    28021 tgaaattttc acctttaccg atatcatcat tttctgtccc attctctttg tcacttactt
    28081 tagcagaaga ggattcgtcg tttgacattt ttgtagctgt tgcttcttct gtttgttggt
    28141 tatctgtggt agcggtcgtt tcttgatctg tgctagtaac agatgtctca accgttgttt
    28201 cagtatttgt ttctgtttgt gaagtactcg atttcgtttc atttccagca tcagtagttt
    28261 ctgtggtttc tacctcccga gattccgatt cggaagaaat gctctctcca tatactgtcc
    28321 attgcgggac gcttgtactt acagcaaata agatgaccaa cgctcccttt aatacttgtt
    28381 ttgttctcaa gctattcact cctttttatg tttctaggag aattatatgt aaacgcaaac
    28441 attttttata caaaaaataa atggtttatc ccataaaaat agaacaggat tattttctca
    28501 ttttacgtaa taaagcagaa tggataaacc agtatccatt ctgctttttt taaacgaaga
    28561 aacaacttat tgctccaaaa atgtcagaaa tcgtttatcc caaattttct cgtaaaaagc
    28621 gattgtttcc tttgctttca taaaaggatt atcataagtt gtcgttgctc ctctacacat
    28681 cataagctgg tatcctaatc catgagcaaa cttcacagtc gtatctatac aatactctgc
    28741 ttgtgcccca cataattcga tcgaatgaca gtccaattca gttaataaaa cactcaaatt
    28801 agtcttataa aatgaattcg catgggtttt tctcacaaaa taactgtgct caggaataga
    28861 taattccgac aatagctccc atggttttgt ttgtggcact aattgagcat cttcgtgttg
    28921 aacgaaaata actggtttcc caatcgtttc gtatgttttg attcttttat tcactaatgc
    28981 tgtcactgtt tctaattcgt gtatcggcga agaatcaaaa catacccctt tttgtaaatc
    29041 gatcactact attgcgtcgg ctgtgtttga catacttgtg ccttctttct tttttcttga
    29101 tttaatagta tttcttatct agctagttcc attatacgtg acaacatttt cttattttac
    29161 acctctaaat ggcacttttc gactatttct tcatgttatt atttcataaa tacttagcta
    29221 ttggaggaat gtatgatgat taaattgtat acaggttctg gctgctcgtc ttcaagacgc
    29281 gcaaaacgtt ggcttatcgt ccatgatata ccgtttatcg aacagaactt aattcgtcaa
    29341 ccactaacca aaaatgaatt tatccactta ctaaaattga caaacaacgg cgtgaccgaa
    29401 atgctctcta ctcattctat tagttataaa aatcttgttc acagaataga tgaactcagt
    29461 ttgagtgaac tatatattga gattcgtaca tccccttctt tattgaaaaa accgattatc
    29521 tacgaccaat ctgcaaaact tttgactgga tttagcgaag ataaaatcag gatgttcctt
    29581 tctaaaacta aaagaaagca agagctcaac gaactcttgc tttctggtca tcttgtcgat
    29641 tttaaagaaa ataatctttt tgcaatgtct taattttggc ataaacaatc tgcttttctg
    29701 gatctataat atctagtgtt ggtgacgcct tctgttgcca atactttttc ttttttctaa
    29761 taaacgcaaa cagacacctt gaaatccttt agaataaagt cgacgaaaac cattcaaagg
    29821 agtgtttcaa gatgtcctat tcagagttta ccaaagagtt actagatatt ctagacctaa
    29881 atcttacctt tcatgaagat gcctttcgca aagagcgcat taatgacgaa acatgttttg
    29941 tttttgatgg cacattgacc tatcaaccag aggaatgctt ccattgccac tatcaaaata
    30001 agcaaacgat cattaaatgg ggctggaaaa aagtatccat cttattgaat gatgtcagta
    30061 actacaagac cattgtgcgg attaacaaac agcgcttcaa atgtaaacac tgtggaaaga
    30121 cttttttagc agaagattca gttagcgatc gtcgctgctc cattgcccgc agagtcaaac
    30181 aagccattct tgagctatta agtgagccta tttctatgtc tttgattgca cgtatgaagc
    30241 acatttctcc gaccactgtc attcgaatac ttcgcagcct tcggcctaaa accgtttctt
    30301 tgaaccagcc gttaccagaa gtggtgtgtt tcgatgaatt caaatcggtg aaaaacgtct
    30361 cgggagccat gagctttgtc atgatggacg gcaagaccca tcaactgatt gatattgtgg
    30421 aaaatcggca actcaatcct ttacgcgact atttcttgcg ctaccctaga aaagtccgtg
    30481 aacaagtacg tctagtggtt tcagatttct atacacccta tcgcacattg gtgaaagagt
    30541 gttttccgaa tgcccgaatc gtcgccgatc gctttcatat tagccaacac atcggtcggg
    30601 cctttaccaa ccaccgaatt caagtaatga agacctttaa aaaaggggat cgtcggtcga
    30661 agcacttaaa gaaatactgg agactacttc aaaagaatgc gtgggaattg aatggtcagc
    30721 atcgctactg gcgtccgtca tttcgagatc atctgacaga agcggagata gtcgatcggc
    30781 tattatatta tgatgactcc ttaaagcgag gctacgaagt ctaccaggtt tttctttccg
    30841 ctatcagaag acaagatgtt cctgaattcg tcgcgctcct aaaagaagac tacaaggagt
    30901 tgccagagca ctatcaacca gtgttcacga ccttcaagaa atatcgaaca gaaatcaaac
    30961 gagcgttgcg agttccctat tccaacggcc ctatagaatg tttgaacaac catatcaaag
    31021 tactaaaacg aattgcctat ggctttagaa actttcaaaa ctaccgagaa cgaatctttt
    31081 tatatcgagg aaaatatttc aagaaaacta agaacactac ccaactgact aaagccagaa
    31141 caacaacacg gctagacaag atagctgtct agccgtgtga gtatctcgat tattctattg
    31201 aatttcttca ccaacactca ttgacgaaga gccgcttttc ttaattaacg aaatccaatt
    31261 tttccaatat ggatctattt ttctcattcc tgatttttat gatttcagct ttcagacgcg
    31321 cccaatcatt ttttgtaggt tgtccattcc agtaaaatat ctgtgtattc gtttgatcat
    31381 atgccttttt gtcgatagga taatcgctaa tgacaatatc aggtaactga tcagcggatg
    31441 cactatattt tattgggcgt tcagtcatag ataatatcct tttctttgcc cataattcct
    31501 gtgttttacc atactttgat tgaatataga atatgacagg ttgttcgtgt tttataaaaa
    31561 tatccctgac taacatacag tattgaaaaa tcaaccgtct gttcttttca taataagtat
    31621 taagctctgg aattctgcga gctaccccac taaaaaactg ttgcatgatt ggatacatca
    31681 ctggaaatat atgctgaaaa tctctgtctt ctgctgattt tccaaaagca tccaccttct
    31741 tttttgtccc aaaaaccctt gaatacaaat ggatcattgc caaattgaca tatagataat
    31801 ttttttctga agttgtaaaa caaatcttga agtttttttc aaaattttct acaatagacg
    31861 attcaatcca gccccaactt tcttgtactt tatctggagt atccaacaaa tcttcttcca
    31921 aataggtgtt cattacacca aacataaaat aaaggtaagc ttgctctgtt ggagtgatgt
    31981 gattcatcga ccagaagttt gcttcaaaaa aaggctctag tatgtaatgc gtaaactctt
    32041 cttttgttat caatggatgc acaaatttgt tcatctcttc tgggacatac gtaatcgagt
    32101 ggccttgttt cattcgttgc aacgaaattc ttagtaggct gatccatttc tttctatcta
    32161 tactacgcgt atatggcgca tgtttcgcaa atttttctaa tagttgacgt ttatttttta
    32221 tacttgataa tatatcagaa tgattttcgt cgaatgactg ccaatacatc aagtgaaaaa
    32281 agtatcggat acggtattct tctccttcta tcggattacc ttttttcaag ctgcaagata
    32341 aatgatagtt tttcaaaata gtattaagtt ctcttaattt tctttgaatc gtacgcaaac
    32401 ttatataatt ttttgactaa gattttctaa ggattgataa gtacctttgt aaagtgaatc
    32461 cagtaaaata taaccaatcg ttccctctaa aagtatagaa taaactttac tcagtaaaaa
    32521 ctgagaagaa aactcgactt ttatcatctt gttttctgaa agaaaagata atttatattc
    32581 tggatacctt tcgcaaaacg attctgcttg ttgaatcacc gaattcatct tagccagggt
    32641 gtagtttaga tctaaagcga tatccttcac cacatataat ttctcgtctc tttcataaca
    32701 atagttcaaa aattgatata cttgcctttc attgatatca ataaattctt caaacaaagt
    32761 acatactcct ttttatctat tatttagact tattccgaca gctacatgaa taacgtacaa
    32821 atagattcgt tactgatttt tcagagatac taccatgcgc agtgtgcatt gtcaattatt
    32881 cttgctctta ttttccattt gtgttcctgt tatttcctgt ttctcaaata tgttagtaaa
    32941 caaaaataga tctggcaaag ctacaacttc gccaggcaat acagcactct cgaatgaaat
    33001 attcactcaa aaatctatcg ttttattcaa ttttagatct gttgctttta ttctttattt
    33061 cactcgatgg aagagagaac gaaaatagct tgctcctaca atcaaaatga gtaacacacc
    33121 catccatgta attagcttgt tcaaggttct actaccagtt tctggtaaat cgctttttct
    33181 attatctgtt tctttcgctt tttcttgatg atcaattcca cgagaaacaa aatcaggctg
    33241 ttttagcaca ctttttaggt tgctccctgc atttctcttt tgttccttag ttggtatgat
    33301 aggtaccaaa tcaagttcat tatttacttt tcctgtgctg ctttcgctgc tcgtgcttcc
    33361 tgtgctactc tcactactca tgcttcctgt gctactttca ctactcgtac tctccgtact
    33421 gctttcgctg ctcgtgcttc ctgtgctact ttcactactc gtactctccg tactgctttc
    33481 gctgctcgtg ttttctgtgc tactttcact gctcgtactc tctgtactgc tttcaccgct
    33541 cgtgttttct gtactgcttt cactacttgt gctatccgta ctgcttggcg gtccaacaac
    33601 tgtccaactg cttggtttca aattctccgt gatcgtcatc tgtccatcat aaaaaacaat
    33661 caaatcaact ttatctttac tcttgatctc atcaatcgat agttctgcag aaatcattat
    33721 tgtatactca ataccagcgg tccattgtac gtcttttaaa taaacttccc caataacttt
    33781 ttctgaactt ccattaccat cccaaccata tgttagaaca gcatgtacat cactagcatt
    33841 cacgcttttg tttggtttga ctggaatagt aatgacttgt tgtgtcatgt cataaacaat
    33901 ccgatcagtt ttcacagtaa atgaaagatt ctcatttgcc cgttcatttg cagaagtact
    33961 gattccaatc attgctgcaa ttatactcag cccaagaata acaaataata cggtcgctgt
    34021 tttcttcatt tctatcactc tccatataat taccttctat ttgataaagg aattatataa
    34081 aatgaaaatg atgagaacac gacagtcagt tgtgaaaatc taacaaaaga tgtcgtgtat
    34141 tcgcttgaat ttgtttactt tttcgtttaa tcaatgacag acttaaaaaa gcaagaccga
    34201 tatcagcctt gctttcttca tcttttattg ataaacagca gtgtatttct tcatccgctt
    34261 tgttttatat gcatagagta acaaaccaac aatcaagact aacaagaata atgtagttga
    34321 tatagatgct tcagccccaa agtttccccc agtaattatt gtattttgcg attggatcag
    34381 agtggagaaa aaaccttgtt cttgcgttcc gctgactggt atacccaaaa tagctccttg
    34441 tgtccaattc caagtaatat gagcaccaat agccgtaaat aaacttcctg tactgtagat
    34501 aagtgcgctg aaaaagcaac ctgctaaaaa gatattgaaa actgccagaa atgtcatgtt
    34561 aggattcaat ccgtgcaaaa gagcaaataa tgcagcagat ccaatcattg ctattaacgg
    34621 accaaatcta agcatctgat gctgaaaaaa gccacggaat aaaagctctt ctgatgcccc
    34681 cactgcaata aataaaataa aactcgttat cagcggcaca atgtttgcgt ttcctgaatc
    34741 taaccgatac gcccccgtca aatataatgg actagtaata agcagaaaca ctccaattcc
    34801 aacaacgaga cctcccgcta aataccccca gtcttttcct cgaacagaaa caccataatc
    34861 ttctattctt tttccttcga ccttactagc aagaaataaa gctagtaaaa aaacagctaa
    34921 atgtccgacc gtaccggcaa ttgctccaat ccattcacta tttccaataa acatatccgc
    34981 aagtgcatta ttgacaagaa ttcctaaaac aaaaccaact aagactatta aaacagatcc
    35041 aatcaaccga ataatcctga tcatctttca tccctccttt atattaagtt taacaaccct
    35101 tttctttctt gtctcgtgaa aactatgtaa atgttctcca tcatagagag aatagatttt
    35161 tacgaactaa gaatatctac agcaacctta cgaaaatgaa agactgtctc cctaattctt
    35221 ctgttagact gtttttaata aggaaaggat gggaaatttt ttgaaaaaac aactactaat
    35281 tatgtgcctt tcagcatgtg caattttact attctcaggt tgtagcaagt ccaatgaaac
    35341 gattgcaacg tttaaaggtg gaacacttac agttcaggat ttttatgata acccaaaagt
    35401 taaatcaaat aatcaaacac tattaaaaaa aatgattgtc ttcaaagcat ttgatgatgt
    35461 atacggaaaa gagatttctt caaaggaagt aacaaaagaa tataatgaac aaatcaaaaa
    35521 actcggtcca aactacaagg aacaattaaa agcagttggc caaacagaag agacatataa
    35581 gaaactcttt aaacagatgc tggctttcca atacggtttg aaagccaatg taaaattaac
    35641 agataaagat ctagacactg cttggaaaga attctatcca gaagttagta cacagatcat
    35701 tctattttcg actaaagaag aagcagataa agcaaaaaaa gaagcaaatg aaggggaaaa
    35761 tttctccaag cttgttcaag cttatggaaa aaataaaaca ttaaaggaaa ctgatggaaa
    35821 aatgaatttt gattcaacca atccagagat tcctactgaa gtaaaaaaag cagcatttaa
    35881 actaaaaaac ggggaagttt ctgatatcat tcctgtaaca gatccaacga cttatcagca
    35941 aagctattat ctagtaaaaa tggtaaaaaa acaggataaa ggttctaata aagacaaata
    36001 caaatctgag ctagaaaaaa ttgctaccga agcaaaaact cttgatacgg aattcatgga
    36061 taaaacaatc cgtaaagtga tgaaaaaaga caatgtaact attaaagatc cttatgtaaa
    36121 aaatatattc aaataaacaa atttgaccca attggggatg tggcaaaatt tgagaagtga
    36181 tttttggaaa ttccttctta tttttgaggc aaaccacttt tgccacaact tcatcggggc
    36241 ctttttttat ttgtaccaaa acaaatcact ggatttgtta caatcatgag gatataattt
    36301 aaaaaagttt tttatttcta tcacatgaat caatgcgcca ttaagtagat tctttcatgc
    36361 ttccctattt tttcacttgc cgtatcgctt caaaaacaac tatcataaac tcaaactcga
    36421 ctgaaaagtt attttaaaaa agactaaaaa tatcccttct ggacttgttc ctcttattct
    36481 gttatactag agaaacacga aagatatttt tatgcgacac ttttcatttg tttttatgaa
    36541 atttattcaa aactggcgta acttcagagt tacaaggatg tattagaggt tagggaatac
    36601 atcgtttaaa tgagaaataa gtaatagtca cccaaacttt aaattaatga atattgaatc
    36661 gtcagatagc ctcctcgagt tttcgagaag gctatcgttt attttatgga gaaattgatt
    36721 cagaagtaat cagcgacctt ttttagttca ccaatcgttt ctgcttggaa agtttgcacc
    36781 caatcttctt ggaagcccct cactgcttta tcaatcgagg gcatctctaa tacttgatct
    36841 agtagctctt tacctaacgt tatcgcatga gctccagatc gaatggctgt agtgacttgt
    36901 tcacaatttt tgaaactagc tgctaatatt tttgttggtc tttgcgtacg ttcgatctca
    36961 gtccgcaacg aatcaatcac tttcatagca tcaatgcctt gattttgcat cctgttataa
    37021 taaggtgcaa tataatctgc tccattatta atagctaaat acccttgtaa ttctgtgtaa
    37081 atagctgtac cggtaatcaa ataaccttct tttttcaact ttttgatcgc ttgaaggcct
    37141 gcttgattca ccggcacctt gataaacgta tcttttccca gttcttctat gataactcga
    37201 gcatcctcta tcatttcttc ttccgttctt cctattgtct gaatatggat aggaatctgt
    37261 ccgattattt ttttgatttc tttcatatgc gagaagaagt ttgacgctcc ttcttttttc
    37321 agaattgtag gatttgttgt tacgcctgtt aaattcaaaa actgttgaca agaataaata
    37381 ctttttgtat ttgctgtatc aagcatgatt tccatatagc tttcccccat tctgatcctc
    37441 ttgatcgatt ttaatgattg tcccctttct taaatgcggc atttccttat cttctacata
    37501 cattgttcct ggaagttccg gctcatgaga tccatcaaat tttatcgtga tatgtcctaa
    37561 ttcattcaaa ttagtttgga caacatctcc tactgctgtg attacataca tttgtttatc
    37621 aaaacagagc ttcatatcac aagttatttc ttttcttacc tgattcaatt caatcttata
    37681 acaataatct gttaatgttt ccggtgcttc atctccaaat aaaatcatta ttttttcttt
    37741 cctgaatact tctgcttcta taccaacatc tgttacaacc gtttcaaaga tcgtcatctc
    37801 tgttcctcct cgtaaaatat catttgtcta atagttttta ttcaaaatgt ttctagatta
    37861 taaaatttat tgaataacac attcctaacg gaatttgttt cgatattgtg tgatttctca
    37921 gagtacaatg gatgtatgga
//
LOCUS       DAJSBL010000024        37843 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.024, whole genome shotgun sequence.
ACCESSION   DAJSBL010000024 DAJSBL010000000
VERSION     DAJSBL010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37843
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.024"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(253..1197)
                     /locus_tag="ONT85_001441"
     CDS             complement(253..1197)
                     /locus_tag="ONT85_001441"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HCR2378423.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(1493..2437)
                     /locus_tag="ONT85_001442"
     CDS             complement(1493..2437)
                     /locus_tag="ONT85_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR2378424.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            2658..3872
                     /locus_tag="ONT85_001443"
     CDS             2658..3872
                     /locus_tag="ONT85_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR2378425.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            3894..5909
                     /locus_tag="ONT85_001444"
     CDS             3894..5909
                     /locus_tag="ONT85_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HCR2378426.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(5992..7365)
                     /locus_tag="ONT85_001445"
     CDS             complement(5992..7365)
                     /locus_tag="ONT85_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HCR2378427.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(7391..7675)
                     /locus_tag="ONT85_001446"
     CDS             complement(7391..7675)
                     /locus_tag="ONT85_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCR2378428.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(7677..9692)
                     /locus_tag="ONT85_001447"
     CDS             complement(7677..9692)
                     /locus_tag="ONT85_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HCR2378429.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(9871..10176)
                     /locus_tag="ONT85_001448"
     CDS             complement(9871..10176)
                     /locus_tag="ONT85_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HCR2378430.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(10265..11296)
                     /locus_tag="ONT85_001449"
     CDS             complement(10265..11296)
                     /locus_tag="ONT85_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR2378431.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            11503..12378
                     /locus_tag="ONT85_001450"
     CDS             11503..12378
                     /locus_tag="ONT85_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCR2378432.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(12530..13372)
                     /locus_tag="ONT85_001451"
     CDS             complement(12530..13372)
                     /locus_tag="ONT85_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HCR2378433.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(13464..15341)
                     /locus_tag="ONT85_001452"
     CDS             complement(13464..15341)
                     /locus_tag="ONT85_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HCR2378434.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(15338..16759)
                     /locus_tag="ONT85_001453"
     CDS             complement(15338..16759)
                     /locus_tag="ONT85_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HCR2378435.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(16762..20151)
                     /locus_tag="ONT85_001454"
     CDS             complement(16762..20151)
                     /locus_tag="ONT85_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HCR2378436.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            20408..21799
                     /locus_tag="ONT85_001455"
     CDS             20408..21799
                     /locus_tag="ONT85_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR2378437.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(21872..22519)
                     /locus_tag="ONT85_001456"
     CDS             complement(21872..22519)
                     /locus_tag="ONT85_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HCR2378438.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(22527..23213)
                     /locus_tag="ONT85_001457"
     CDS             complement(22527..23213)
                     /locus_tag="ONT85_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR2378439.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(23219..24292)
                     /locus_tag="ONT85_001458"
     CDS             complement(23219..24292)
                     /locus_tag="ONT85_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR2378440.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            24514..25188
                     /locus_tag="ONT85_001459"
     CDS             24514..25188
                     /locus_tag="ONT85_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR2378441.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            25193..26533
                     /locus_tag="ONT85_001460"
     CDS             25193..26533
                     /locus_tag="ONT85_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HCR2378442.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(26682..27668)
                     /locus_tag="ONT85_001461"
     CDS             complement(26682..27668)
                     /locus_tag="ONT85_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HCR2378443.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
     gene            28392..29423
                     /locus_tag="ONT85_001462"
     CDS             28392..29423
                     /locus_tag="ONT85_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR2378444.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
     gene            29532..31064
                     /locus_tag="ONT85_001463"
     CDS             29532..31064
                     /locus_tag="ONT85_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HCR2378445.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIPILYLMEQPALSVSQSFLD
                     KNEISYMTHTSGTTGIPKLICHSAHSMGWRTKWQKTVFTKISEKKLIAFHISPVHSRF
                     NIGISSLMSMGFPMMPLANAQSSKVVHMLEAHRPIALETHPNNFVQWRFTAKEHPEAF
                     SGIRYYHSTFDAINNQTMKTFLRTSMAQDAVFLQVYGQSECGPMILKAHTLESLKTSD
                     ARDMGVGLEDMTSARITDSVGNVLPENTDGHIQLLSKGRALTYYKEDARFQENVYGDW
                     WDSGDYGFMDKHGHLFLKDRQVDLIETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSP
                     QPIIALAPDKEMDWNRWWEQVHELPRLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQ
                     KS"
     gene            31203..32015
                     /locus_tag="ONT85_001464"
     CDS             31203..32015
                     /locus_tag="ONT85_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HCR2378446.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            32005..32490
                     /locus_tag="ONT85_001465"
     CDS             32005..32490
                     /locus_tag="ONT85_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HCR2378447.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            32513..33058
                     /locus_tag="ONT85_001466"
     CDS             32513..33058
                     /locus_tag="ONT85_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HCR2378448.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(33131..34774)
                     /locus_tag="ONT85_001467"
     CDS             complement(33131..34774)
                     /locus_tag="ONT85_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HCR2378449.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKHALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(34903..37131)
                     /locus_tag="ONT85_001468"
     CDS             complement(34903..37131)
                     /locus_tag="ONT85_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR2378450.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKERTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            complement(37164..37643)
                     /locus_tag="ONT85_001469"
     CDS             complement(37164..37643)
                     /locus_tag="ONT85_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2378451.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNMDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
BASE COUNT        11028 a         7173 c         6896 g        12746 t
ORIGIN      
        1 tagatatgaa tcattgtatt tgccgttgcg gaaggatttt ttaattaacc acagataaac
       61 attgataacg attcgcttat tgtaatcaac agattaaatt tcatatgttc atatgtacgg
      121 atacattttc ggaccgatta attttaacca ttctgtttat ttgcttacct attagatatc
      181 aataaaaaac tgcttaacag ctatttcagc tgttaagcag ttttaggatt tttagttcat
      241 attcattgtt tcttataata cagtgttcaa tacttctctt aaagcacttg ttgatttgct
      301 gaacattgct ttttcttctt cgttgattga taattcaaca acttcacgaa caccattttc
      361 gtcaacgatt gatggcacac ctgtaaagat atctttttcg tcgtattcac cagttaaata
      421 agcagataca ggcaatacag cttgttcgtt attcaagata gcttttacga tacgtgcagt
      481 actcatacca ataccatagt aagttgcttt tttacgatca atgatctcat aagcagcatt
      541 acggacttta tcagcaatca catctaattc atcttgggca atacgatggt ctttttctac
      601 aatttcaaac actggtttac caccgacagt tgtatgtgac catgcagcaa cttctgaatc
      661 accatgttca cctaagatat agccgtggac gctgcgtggg tcaactttta atttcaatgc
      721 gatttcttta cggaaacgag ttgtatccaa tgttgttcca gtaccgataa cacgtgatgt
      781 tggcagacca gattcgttcc aagcgatata tgtcaaaata tctactgggt ttgaagcaac
      841 aacgataata ccgtcgaatc ctgatcccat gatttctttt acgatttggc gcatgattga
      901 cgcatttgtt ttaactaaat ctaaacgaga ttgaccaggt ttttggttga taccagctgt
      961 gataacaacg atatcagcat ctttacattc ttcatagcca cctgaccata cagccacatt
     1021 ttcatcgccc caagccatac catctaaaag gtccaatgct tccccttctg ctttttcttg
     1081 attcacatcg atcaatacta attcattaga gattccttgg ttgatcattg cataagcgat
     1141 acttgttcca acaaaacctg taccgacgat tactacttta cgacttgttt ttttcatgaa
     1201 taaacagctc cttttcataa ctatttgtta ctttcatgaa aagtatagtc ttgtttattc
     1261 attttatcaa catgttaatt ataatagttg ttattataat ttttgatgga aacgaattcc
     1321 tgaatacgca taacagataa atgacaaatg tttacaccta tatcccctga taatacaacc
     1381 aaaagtttag aactgaaaat aaatccaaaa aaattcgctg caacgatata ataagacagc
     1441 aatcgcacct aaaacaattt tccttcatat gctttagtga aagattttct tcttaatgtt
     1501 tatttctcca ttcattggga gtacatgttg aatacttctt gaataatttg aataaagaag
     1561 tcgtttgttt atatccgata cgattagcga tttcttccaa cgaaaaactc gtttcttgaa
     1621 tcagttccaa cgcaaagtcg aattttttct ttagaaccag ttcttgaaag gaagcatcaa
     1681 aatacatttt caatttatta ctaagataga ccggattgta gccaaaatga gctgctgttt
     1741 cttttaacgt acaagaagta aaatgcttat cgatataatc taatactcta agcatctcat
     1801 tttctgcgca tccatctgct gatgaaaagc tttgttgttc taataaatga gtcaattcga
     1861 tcagtaatag tgatgtatag agatttaacg aacggatctt atgactgtat gtatcaaaat
     1921 aaatcaacag catattttca atcaaatgcg tcaaacgact tgtttttccg ccgcgaaaat
     1981 gaatgaattg tgtatgttcc cctttttctt ttgatgcatt tatcaaaaaa gaagaaagta
     2041 gattatttgt gcttgccaaa tgattcaata tctctgtaga taaggtttct tcttttaata
     2101 agatgttgat taaaatatcc tcttgccctg tgtattcgat tctttgagta gcttctttat
     2161 caatgatcac cacgtcactt tcttccatct ctatcctttc tccattaata atttgtaaac
     2221 atgtaccgct atacatataa ttgatttcaa caaaatcatg tgtatgctcg ggaacttccg
     2281 catatcgacg gtgtttactg atatgaatat ttccctgttg aaatagatcc cctcggtgga
     2341 tttttggcgg tgtctgaaga tggtaagcta agtttaaatc attgatattc attccttgtt
     2401 cacgctgcag agtttcgatt gattcttcgt tacgcaaaaa aaatgtctaa ttcagctagt
     2461 tccaattgac tccctccttt ttgtaagcgg tttctttcgc ttattagttc acgctgtact
     2521 gctattatat gcaaagctat ttttggtttc ataaagactt tctatggttg ggacatgaac
     2581 ttcctgtgat tccgctattg agagaataac cacttcatac tatatttttg ataagcgctt
     2641 acaaaaatga aaggaacatg cttatgaata aaaagatgat gaaattttct ctgctgtcag
     2701 tctctcttat tttgacttct gctggtgcag tcacgtctgc aattcccagt atgtcagcta
     2761 cttatgccac gcgttcttta tctgctgttg aattattaac gactgctccc tcttttatgg
     2821 tcactatttt tgttttgatt agtagtttta ttgctaggaa aatcggacaa aaacaaactg
     2881 tgatcatcgg attagtgatt gcatcgattt gtggtatcct ccccctcttc tcacaaagtt
     2941 acagtattgt cttagcttca agagctggac taggtattgg ctttggattg atcaattctt
     3001 tggcagtcag catgattgct gccttttatc acggagatga acgagcctct atgattggtt
     3061 ttcaaagtgc atttcaagga tttggtgcag ctttaatgac atttgttgca ggacagctag
     3121 tacagttcgg ttggcaatat acctttttag tctacgccat cagcttacct attcttgtct
     3181 tgtttgtttt gtttgttcct acacctgaaa caactgaact tcaatcgcat ggcacagccc
     3241 caacaagaca agtaatcaaa acgaatccta aaatgattag ttatggtatt tttctcttgc
     3301 tggtcatcgt ggtatataat gctgtatcga ttaaattagc cacagtcctg acgacttatt
     3361 ctctaggaaa cgaaactaat gcagcgaata tcttaacaat catgcaattg gcaagtatgg
     3421 ctgccggagt ttgttttggc tggcttcaga caaaagcaca tcgctatatg cttacacttt
     3481 ctttactact gatgggaatc ggctttgtat tgattgccac tgtgataaac ctgtatgtga
     3541 ttgggtttgg tgcgatccta acaggaattt ctttttcgtt attcattcct tacttattca
     3601 accaagtaag tatccaagcg cccaagtacg cggagagttt caatacttca atcttattgg
     3661 ttggcgctaa tttaggtact ttcattgcac catatggttt gctctttctc tcttttgctt
     3721 ctcttgggag taaaacactt gctgttttcg tcaatggcag tatcttgctg ttgattagcg
     3781 cgcttattag ccttctagta gtacacagga cgaatagaaa gacagtgata agagtagatg
     3841 aagaaataaa agaacaattg acaacaaaat aaatttgatg gaggtcgtgt aaaatgacaa
     3901 atgaaaaaca taaagataga tggctttggt atcctggaga ctttgagatc cgccatggac
     3961 tgctacaaaa tttccaacgg gaagaacgag gttttgactg gccagcgtac tggtacatgg
     4021 atgattgcca tcggaacgtc aagtttaaga gatattactt tcttgatcaa ccttccatgt
     4081 ttaaagtgac gatccaagga gttgggtatg tagaaattaa tggacaaaaa catccatgtg
     4141 gaaaatggct gacttgtcca gctggtaaag caaaaattcg tatctttgta ggacatacat
     4201 cagggctacc agcgatgttt atcgagggag acgaagtaaa atcagatatt ggctggacag
     4261 caagtaattt tattgaagaa tatccagctg gctggagtcc gctttatgtc gatatcgcaa
     4321 aagatccaaa tcaaatctat tatcaaaaag aatgcgtcca tccagtcact gaaacggaga
     4381 ttggcggagg aatcttattc gattttgggc gagcagtgaa tggctgtgta gaactaatat
     4441 ctctggatga ccatatcgaa tctatcactc tatgttatgg agagtccgat gtagaagcta
     4501 tggatatcga ttattgctac tataagcaaa taggaatgac aagaaaaatg aaagctcgta
     4561 aacgcgcttt tcgctatttg tttataccag aggtttctac tggaatggtt caacttcaag
     4621 tttttcatga atttttatct ctttcgaaac aagctgcttt ttcaagcgat tcttctttac
     4681 tgaatcagat ctggtcagta tcagaagaag ccttcaagtt atgtagtgat ttgtttttta
     4741 tcgacggtat caaacgagat cgctggattt ggtctggaga tgcgtatcaa tcattcatga
     4801 tcaatcaata tctttttttt gacgaggaaa tcaataaacg aacgatatta gcattaagag
     4861 gacaaaatga aatcaagcag catatgaata ctatcgttga ctattcgatc ttgtggttga
     4921 ttggtatcga aaaccactat atgatgacga aagactatga atttttacgc atcgtctacc
     4981 ctaagatgca atcattaatg agatacttga tggagcaaac gaatgaatta gggtttattt
     5041 atgggagagc aaaagattgg atatttattg attggtctga aatggataaa gaaggaacgc
     5101 ttgctgcaga acaagtctta ttactgaaag cctatcattc catgatcact tgcggagata
     5161 tcttagctga aacatatgga gagaacgaaa attatcatac tcatctctat caagaaaaat
     5221 atcaactatt gaaacaaaac ctatttgact acttttggga tgaagaactt ggcgctttta
     5281 tcgattgcta tgaatctggg agaagaaatg tgacgcgtca tgcgaatatt ttcgctgttt
     5341 tattcgattt tgctaacaaa gaccaacagc aatcgatctt aaagaatgtc ttattgaatg
     5401 acactatcac acagattact actccatatt tcaaattcta tgaacaagat gctttatgca
     5461 aactcggaca gacgaatatc gtctaccaaa cgattcttga ttattgggga gggatgttat
     5521 ctcatgatgc ggtaactttc tgggaagaat acgaccctac tcaaacagga aatgaaaagt
     5581 acagtatgta tggagatcct tatggaaaaa gcttatgcca tgcatgggga gcaagcccaa
     5641 tctatctttt aggacgctat tttatcggtt tgtatccaac aaagccaggt tatgaagaat
     5701 ttatgataca acctaatcta gattcatttg aaacactgaa ttgtacgcta cctatcaaag
     5761 atggaaagat ttcgcttgaa aaaacaaaag ataagatcgt gattacttct gatagaacag
     5821 gcggaacagt gaaaatgaat ggtaaaagtt atcctcttct agcaaaacag ccacttgtct
     5881 taacaagcta ttcaaaaata aatgcataaa acaaaaaact gcttgcgaca tatcagaaga
     5941 ctttaaatct tcttctgata ttgtccaagc aatttttatt ttgaagtttg cttaattctg
     6001 ttctttagaa acaatgtttg ttctttttcc ggcaaaaaga aagctagata atacgacaag
     6061 taaaaagaaa gcgatgattc caactgtcaa ggctatttga ccgtattctc ctagtttccc
     6121 aagtacaagt cctggtacta gataatcagt atcggcaaat gtagaagaag cgattttaag
     6181 atctcctaat actggaagga taaataatgg cagccaacta atcaataagc tattgaaagc
     6241 agaacctaat accgcccctc ttctaccgcc agtcgagttg ccgaagacac cagaagcagc
     6301 tccgcagaag aaatgtccta ctaccccagg aatgataact gtagtttgca agcctatcat
     6361 agcaaacatg gatagtgttc ctacaaagaa gctaacaaaa aagccaatta atacagcatt
     6421 aggtgcatat ggaaaaacga taggaacatc taatgctggt ttagaatcgg gtacgagttt
     6481 tttagcaatc ccttggaatg ctggaacgat ttctgccaac accattcgaa ccccttgaag
     6541 gacgataaca aacccagctg tgaatgttcc agcttggatc agtgcaaaga ttacagcgtt
     6601 gtctccattg cttagttctc tttcaacaaa tccaggacca gctaacaaag caagtgcgac
     6661 atatacgatc gacatcaaga gcatgatcga tacagtagaa tctcgcaaaa agctgaaact
     6721 tttaggaatc tttatgtcct ctgtagaacg gtctttgttt ccaaaaaact ttccgatcca
     6781 tccactagaa gcataaccga tatttccggt atgccctaac gcaacttgat tgtttccagt
     6841 gatgttcttc ataaacggtt gacaaagagc aggcgtaata gttaatagtg ttccttcgaa
     6901 gaccccgcct aataagatca tccacaaatt tgcagtaaac cccgcgctta ctaaaatgac
     6961 tgctgttaga caagaaacat aaagcattgc ttgacctgta agaaaaatga atttatatct
     7021 tgtgaatcta gcaagtaaaa tattcacgat catccctact agcatgatca aagcagtagg
     7081 tgtaccgtat tgttgcaaag caatcgccac tactgcttca ttgctaggaa ctactccttg
     7141 agtatgcaga gctgtttgga acatcgaagc aaaaggggtt agtgaattac tgattaaacc
     7201 agcaccgccg atcagtacta ggaatccgac aaatgttttg atagttcctt ggatcacttc
     7261 tgaaaatttc ttcttctgaa gaacgagacc aagcagtgca atcaaagaaa cgaggatgga
     7321 gggctgactg acgattgaca caaacaaatt caacaattta tccattgata gttcctccct
     7381 tgaatcattt ttattctttt gacaagatct tcgttacttt ttcttttact tcttcgctgt
     7441 caatgatcga ttgcaaaggt acgagctttt catcaggtat agaagttagc gtatctttca
     7501 aagaatgctc aatgaagaaa taatcagcca tatcgctaga tacacttcca acatccgtat
     7561 gacttacttc aaactcgtcc acatctgctc caagatctgc aagtgcttgt ttgatgttca
     7621 tctctaccat aaaacttgtt cctaatccac tttgacatac tgcaaaaatt ttcatatcat
     7681 tcgtctcctt ttcttatgat cgctaatatt tcttcttttg ttgatgcgtg taataaatct
     7741 tgaagatttt gtttattaga aagtagcttt gttaaatttg ccaaagcccg aagatgtgcc
     7801 tcattatcga tagcagctaa acagatgaac aaactaatgg gatgggctgg attatcaagc
     7861 agatccacag gttgttgaag tttcagcaaa gacatcccta cttcgtttac gccatcttct
     7921 ggacgagcat gaggtaatgc gatgtgatcg ccaatatgga taaacgcacc atactgctcc
     7981 acgcgctcga tcatagcttt gatataactt tcttctattt tattttctga taatagcggc
     8041 tgtgcggcaa atgtgattgc ttcttgccag ttttttggtt gatccgataa ttggatcatt
     8101 tcttctgtta ataattcaga tagcatagga cgttgatcct ccttcttttc taaaagtttg
     8161 ttcattttac gatttaatac ttgatagagc ttttcttcgt ctacgccttc ttttaattcg
     8221 atataaggca gtagcgccga caacaattct tgggcagaag gcattgaaaa tcctggtatc
     8281 agccaatctc tttgtacttg attgatcagc tgattttttt ccaagtcagt catgattgga
     8341 tgcagaacat agagagactt tttcttatca gtaagtgtga gaggtacagt agaaaaaata
     8401 atatcgtaag aagtttcgtc aacctcgtgt aagcggtcag ctgaattagt ctctgtaaaa
     8461 aagatcatcg ggaataattg ctgaagttca ctttttaaga tcaatgaaga gctaatgcca
     8521 cttggacaga cgatcacggc tcttattttt ctatttcttt cttctgcgtc cgcttttctg
     8581 atctctccgc caaatagtat agtgaaaaaa ccaatctctt cttcagggat gctcttccct
     8641 attcgtcttt ctaacggaag taatgctttt cttgtcattt cgtagatgga agcatattga
     8701 tgcttaattt gatcgatcat cacattaggt aaataaaaag aataggaaat acgaaaatag
     8761 gcagggacca agtgataaaa gacattcatc aataattcac gaaacgaagc aaattctacg
     8821 gccatcagga attccatgcg atggatgact tctgaagaac attccagcaa aaaatcaagt
     8881 gcaggctcct ggacatctcc ttcaacgatc gatgctaagc aaagagagaa aaatataatc
     8941 tccgaatttt gatgttcgga atagccaagg ccagtcaata cttgtcgact aatgtcagtg
     9001 actgcaagag aagccaacag ttcagcatct tttaccagtt ccatggatgt ttttccaatt
     9061 cgaatggttg tcaaactcaa ataataagct aattcatcca ttcgatttgg tgcgaatcga
     9121 agatctgata gtgaaatcgt tttatctagc aaatctctaa ctttggcgta ataatcaatg
     9181 gaaagggttg cggttaaata cgtgagccca aatcttccag caagtgactg catcaaacga
     9241 gcagtgcgat tttttgcttc tcttcttaat attgcttctg accctagcag cctaaaacca
     9301 gttttcctag tatacgacag ctgacaatct tccacttcta attcatttct tatctttttg
     9361 atatctgaca ggactgtccc tttagaaacc tgaagaaaat cttgaaaatg aaaaacagaa
     9421 acagacgttg cagtcgaata aaccatcaaa taaagaagat ctcttcgctc ttgttcggta
     9481 tacacagctt caaatcgagt aatatccaaa aatttttctg cccagtcgat tgctcggaat
     9541 ttctttatac ggatacgggt atcatccaca tattcggcaa aaggagttaa ttgtttgatc
     9601 gcctcgcttg tatttacttc gatgatctct gctgcttttt ctagactgat ttctcttggt
     9661 gttttcagga gataactaat tttgtagttc acagcgctgt tcacctccta cttatattct
     9721 agtatggtag cgcttttaaa tcgaggggtg tttggtacaa cttacagttg gatttttttt
     9781 acgactgtac cgaacgattt ctatcgaatg gatacagaaa aaaagcaggt gaccaaatgt
     9841 ctcctgcttt tatgaattga tttcgtccga tcaatgatga tcatgtttat tttttgaggt
     9901 cagttcttca aatttttctt tgacatcttc aaccgctttt tcgatatctt tttcaaagtg
     9961 attgggctca ttaggctttg tttcctcttc tacagaattt gaaccagata aagctgtttc
    10021 tttattttct gtttcaccag agtttgtgcc agctaatgat tcttctttgt cttctgtttc
    10081 accagaatta gttcctgcta aagccacttc ttcttctgtt ttaggggcca ttgaacctct
    10141 taatcgtttg tgtttttcac cgtcataatt tgtcatgttg gttcctccta gtagttctat
    10201 actttttcat cgtacaataa gacagtttat gcgtcaaaaa acaaacccga ttcttacaaa
    10261 tatgtcatat ttttccttct gattcttgaa tgcttttgat tccttcttcc aaaattttca
    10321 tttgtaagat tgtttcttca tctttgatga ctttttctgc gccattaact aatgtctcgt
    10381 aaatcccttc gtagacacgg ctgtaatcac caacctcgga aattactttt tcttcatgat
    10441 aactcccttg ttcatctata taggtcaata caccataatg ttcgggcaaa tcgataccaa
    10501 agtcttcatg atcaggcatg taaaacattt tcagatgttc ttcttgtcga tcttttgttt
    10561 gtttcacgaa catccctttt tttccgtaaa cgacgaaact tggacgttct ttgatgcgga
    10621 aataactgga ttttatcgaa attttcatat ttccatagaa aaaatccaga tcgaagtaat
    10681 cattcattcg tcctttccct aaaagttgac gtacatcata gctgatatca tctggttcgc
    10741 cgaaataaga aagaacttga tctactgtat gacaagcatg tccatataaa tagctgttcg
    10801 cagtagaaaa ttcatgcgta ttttctggta cttccggtct gaaataatca taatgcattt
    10861 ctacttctaa aatatcccct aattttccgc tttcgattac tttttggact gtcaagaagt
    10921 cagaatcgaa ccgtctgttt tggtaacatt ggataaatag acctttttct tttgctaatg
    10981 caaataactc ttctgcttcc tttgacgttt ctgcaaaagg tttctctact agtacattct
    11041 taccatgttc gagtgccatt tttccatatt cataatgaaa ttgactaggt gtagtgatga
    11101 caactaattg gatctcagga tcatcccaaa gatcagacag ctgatcagta tattcgatgt
    11161 cttcgaatgt tttccacact ggttcagatg gggaaaaaat ttttttgata ttgattttgt
    11221 tcgtaagttt tcttgaaaaa ggcaaatgat agcgatttgt actctttcca ttgccaagat
    11281 aagcgattgt aagcatgagt ggtgcctcct tttattgatg ttttcagtat atcctagaaa
    11341 agcgcagaaa gtatttataa tagctattct ggtaaaaaag ttcagaaaaa cacttgttat
    11401 cattcggttt ggtgaaatta atgttcaaag taaggataaa aatcaaaaag atagtagcgg
    11461 aaccaagtgt ttatcagtaa gatagaattt ggaggtcaga tcatgcttat tgaagaaaaa
    11521 ctaacaaaac aagagttgtt taccacgaca gagaaacgga ttgtcgatta catccgtagg
    11581 aatatcgaag ctgcagtcta catgacgatt gaagaattag caaaagctac gtatacttcc
    11641 cattcagcta tcattcgttt atgcaaaaaa atgggattca gcggatttaa agaattccgt
    11701 ttcgagctgg ggcgggaagt tcatcaattg attgctcagt tcaatcaaac gattgatcct
    11761 aattttcctt tttcaagtga agatagcact cagcaaattg caaagaaaat ggcggattta
    11821 tcgatccagt caataaaaaa agcacaattg cagattgaac atgaagattt agatcaaatc
    11881 ggtaaagtat tgaacaaggc acagcgaatc tttctttttg caaaaggtga ttctcaaatc
    11941 acggcaagga aattccagaa taaaatggtc aagttgaata agtttttgat catggctgaa
    12001 gaatacagtg attcttcttg gaatgcagcc aatttgacta gcgaggattg cgccattttt
    12061 attagctaca gcggtcgcat ccatcattat gaacgaataa tgacctattt gaagcatgta
    12121 gaggccccta ccttgctgat taccggaaat cagcattcag aaatggccaa acaggctgat
    12181 atgtgtcttg ttatatcgca ggaggaatat gattttgcta aagtagcgac tttctcctct
    12241 caaattgcct tcgattacgt attaaatacg ttattctcag tgatatattc tcaaaatttt
    12301 gaagagaata tccgcaactt gaaaaacaaa caagaattga tgcaaaaagg ccttcttaaa
    12361 gaatatcgga aaacttaaga aaagatcctc atttagtaat aagaaagata gtgtttgata
    12421 ggctctcttt gatatcatca taaaaacagg ctgaagtttt ctttttcaaa cctcagcctg
    12481 tttgtttacg gaacgattcg aatagatata ttatagaaat gaggaatagc taacttctgt
    12541 ttcttatttt gaacgattga cgaaaaagat atataagaag gaggaaaatc agtatacatg
    12601 cagctaacaa tccatacagg aaaaatcgtt tgttctcgga tactttctgt acttcttttt
    12661 gaacttcttt tgcatgataa ggaacccggt gccctctgac aagtaagcga tgactgttga
    12721 ccatataagg tgtacaagtt aggagtgtca cgaaatcctg ccctttttcg attctcagat
    12781 cttttgtatc agtcggttct accgtcttga tctgatcgac ttgataagcg agtgtccgtc
    12841 cattgatttc gatatagaaa gaatcaccta aagtcagttt cggtaagtct gtgaacaact
    12901 ttgcttgagg cagtcctcga tggctggaaa taacggcatg tgtattttct ccccccgtag
    12961 gataagacgt tccttccaat aatgaggcac ctttttctag cagcacagta ttcgtttcat
    13021 caaaaattgg caaatggaca ttgatctcag ggatcgtcaa aataccgatc gtatgtttct
    13081 caaaataggt tctatccttt ttcgcaggtt cttttttctt agtaaaggga tctgcacctg
    13141 gatttccacc ttcttttgct aattgctggt ttttctttgt catgttttct tggattttag
    13201 ccatgacttc ttcattttct ttgaccgctt gttgctgata atgagagatg atctgctgat
    13261 ccagataatt attcagcgcg tcgctaacaa atggataagc tagtatccct attcctgaga
    13321 gaagcaagaa aagcatcagc agatctagaa gtttcctttt ctttggtttc atttgtgttc
    13381 tccttgtgat gactgtttga taataaaaga aaaaggagca tttgcgtaat ccgctgctcc
    13441 tttttccaat tcttgcaaaa agactaaatc tgactgtgct tgcgtctagc aaagtacagt
    13501 ccagcaatca gtagaaggac tactcctgca ccgatataca catagattcc cttaccgcct
    13561 gttgaaggaa gtgtaccttt gtgtttattt ggtatttttt caggagaaat caactgtcct
    13621 gctgttacat aagattgttc atcgatagta aatgctacac ggtttgttaa tggaacatat
    13681 ttttctggcg ctttcgtttc ttccagatag tacgtgccat attttagacc tgtcacatcg
    13741 attaaaccgt tacttgtggt tgtaaaaacc gttgctgatt ctttcgccga tacccagctg
    13801 acggcttttg tggatgggtc gatcgataaa tatttcgctg tgtcactatc ttgatcacga
    13861 acgacgaatt ctgctccagc aagtgtttgg tctgatgtaa cgtcaccatc tactttaaca
    13921 aatcgtttgc ccccagtaac gacatcgact gacggtggtg tttgatcatt tgtatgcccg
    13981 ttatcgacat ttgcttggtt agaaaatcct ttggttggat ctgctttttc gttcaaatgc
    14041 atatagtaaa cgaatttcaa tgtaccgcca ggagttaatg aaggaatata attcggatca
    14101 actgaaaccg tgaatccgtc tgtttgctca gtgacagaat aatttactgg gtcgatttct
    14161 ttatttccat catataaggc ataagcatac gttcctgaag aatcattatc aaatgttaaa
    14221 gcagcgtcat gagtatcgat aagtttgaat gttgtgtact tgttttgcgt tccttcttta
    14281 tcagcgatac ctaatgggat attgacagaa atctcatatt gaattttttc accgattgcg
    14341 acatcttttc cattcaattg aggtgttgtt ttatcaactt tagacgtatc atttttgatg
    14401 gtcttttcta ctggtgtttg gctatttgcc aggatctcaa aaggcgtgat tgtttgcttt
    14461 tcaatcattt ccgcattatc aggagctttt acttcttcta aattatattt tccaacttct
    14521 aaatgattaa cgatcaactg gcctttttca atagatgcct cggcaaagtc attgttgccg
    14581 atgtcataag aatgaccagt aatgaaatgt ttggctttgg tttgatcagt tgtccaagtg
    14641 tacaatccat ctgtgacact ttggatgtat tttttgacgc ttggtgttcc ttcttcttta
    14701 gaaataataa attctgctcc atttagtgct tcgttttcgg cagtaccgat ttttgtaact
    14761 ttcaacgttc catcattacc gactgtattt ttagggtaga gatggatagt atctagttct
    14821 tctgtcccgt atttataaga gccatctgct tgtttgatca tctcatatac agggaaagct
    14881 aaaaccatgt ttgcggcagc tgacactccg tcttttggtt cttctttgat cgtgtagact
    14941 gcatctttcc cattttgttt tttaggtaaa gataaagtga catttccatc agcatctgtc
    15001 gttcctgaag caactggtgt accaggagtc aaagactgga ctgcttgttt tgctgcatcc
    15061 acggacgctc ctttatctcg ttgcgcataa aattcttgag tgacgttata aactgaaaat
    15121 gaaatatcgg ctaatccttg gtattgatcg aattcgctca tttctttccc gctgttttgg
    15181 attaaaggat cgggtaaatc agtcattttc tttttatgaa ggatgacttg agctgctgtc
    15241 tcctctgcaa acactttttt tgcgccgaag ctgtttgtga gtaatggtaa aataaggaaa
    15301 aggactccta acataacgtt tattttttta tgatttttca ttttttcact cctgttcttt
    15361 ttttgataaa caaccagatt gcaaagcttg cgaataccat accaacaacg cctaaaagtg
    15421 tatttttcgt ttcgtttgtt tttggaagaa atggacggct attttctttt ttattgacta
    15481 ctttcaatat actgtccgcc tctttatcct cagaaagaaa agttgaaatg gtaaacggaa
    15541 tttctgtttc acttaaaata taaccagaag gtgcttcgat ctcttttaat cgataacttc
    15601 ctgttttcaa cccggaaatt gaaaaagcgc catttttatc ggaaatcagg tgaagcgcat
    15661 ctgattcgtt ctttgtccac ttgtatccgt tggctgtttg ctggaggtaa tttcctgctt
    15721 tgtttttaat aaggaatact gcgccagcta atcgtttatc tggtttattt gtctcgattt
    15781 tgacaaattg cttactccct gtatatacag agactttctt tgtactgaca ccaaaaccaa
    15841 aatccagtgt tccgttgttg ttgatttcct tgttcgcctg tgctgtactg cttagctgca
    15901 tctgataaga aatggtcagt ttttttccag catatttttc caacgaaggg atcgaaaata
    15961 aaacatcaaa tccgtgaggt ttcttgtgta tgacaaagcc ttctgtcagc ttttctccat
    16021 tcgatgaaat cgttaaacta ttaggtaaaa acgttaatgc ttcatccgca ctatctgaca
    16081 attcgaactt ttgatagtca aggatattta caggaattgt cgtatgcaag gaataagtga
    16141 ttttttcacc aatcgtaaaa tcatttctag gctcttctaa taccttctca aaaggtggat
    16201 cagggtagtc gttttcttca tttttaggat acaaatggat agttgaaagt ttttgattgt
    16261 tttgtccatg tacaggaaga acaactacca tattctctgc cttttctttg acgacttctg
    16321 gtactttgga ttcaatgaaa agatacgctt tatctctttt ttcttgatct ttagcggcca
    16381 gagaaaaaga agcgatacca tcttcgttgt taagagttgt agttgttgct tctgcggtaa
    16441 acataccgga agacgcaccg ttttttgcga tctctgcttg tgcttcttct accgttttgc
    16501 ccttttcccg tagatggtaa aaagaatctg tgacatcata aacttggaat gtgacaccat
    16561 ttaatcctcg atacgtttgt aataaagctt tttcttgtcc gtcatttgga tgatttttcg
    16621 gcagttgccc gtttgggaaa agcaatttgt gtaaagtaat ctgaaccatt tctgtttctg
    16681 tttctgttgc tacctgagta aaagctggtt taaatagtaa caagaagaga cacatactaa
    16741 gccaaccaag ttttttcata tttagctgcc ccctctttct tttttcaaaa aaagatacca
    16801 aatagaaaac gcacaaccaa tcatccctac tagatagatt cctaaacggc caattcctcc
    16861 cgtttcaggt aatggtactt ttgcctgatt cgtgatgtct aaagaaatct ggttgttttt
    16921 ggctcctggt gacagaacag attcatgatc ttgtccatcc acttgaattg acccatcttc
    16981 gtgtataact atagtaactg gctcttttag accttggtat ccttctggtg taaaagtttc
    17041 ggtcagcgta taagttccag gttttaaatt ttcgaataga aaggtatctg tttcttgtcc
    17101 atcttttggc gattctaagt ccatttctgg tccctgcagt ctgaattttg ctccttttaa
    17161 tgtctgtccg ttgtcttctt ttttgtgtac cgttagatca aacgctttta gtcgattttg
    17221 atgagtgagc gtccatacat tatttgtcat ctcggtattt tttcccgtgt aaatgatttt
    17281 tccatattta tcgatttgga attcataatt tcccggcgaa gtatcatatc cggcaggacc
    17341 agctgtttca acgactcgat atttaccagg tgtgagtgaa tcaaaactgg caagaccttt
    17401 ttcattcgtt gtttggctgt caattggctg ataagtacca tttgcttctt ttttctgtag
    17461 ggaaaatgct gctcctttaa gatttatctc tttgtccgtc ccttgcatcg catacttacg
    17521 tactgctaca ggaacttcaa caaaaggatt ttcaatagtc aactggatcg tatcatcgga
    17581 agtagtgact gtttttccat caatggttac cgtaccatca gaagcgatct tgatttccca
    17641 agtagtcttt ttgcttaatc catgcccttc tggagctttt gtttctgtca gcgtataggt
    17701 catttctttt tgcaatttga cattttctgg aagagaatag gttccgtcgc catgatctgt
    17761 tagtaaagta tcaatagaat ctcctgttaa tttgaaaaca gcgccaataa gatccttttc
    17821 acctgtagag gaatttttcg ttattttcaa gtttaacggt gtgaattgtt tagtattttt
    17881 ccatgtcatt gcatctattt gttgagaatc gtatcctggt acaggtaatt ctttgattgt
    17941 ttggtaactg aatgcttgac cttgattatt gtattgaggt agtgataata tctcttgata
    18001 actttctccg ctatttgcag ataatttgtc aatgtctgca cgttcccacg tatttgttgt
    18061 atctttagct ggtttaatga ttcgaatata tccgtttttc caagctgcag catttgtcgt
    18121 atgttcccgt tgaatctcaa aagtaacttg atctggacga tcagctagat tgttgtcaaa
    18181 ttcttcccat aactttttga tgtgaagact gactccggga gctttagcag atggtatacc
    18241 gaattcagct aattcattgg tatcaatgtt tggctggaaa gttgtcctgc cgttcatttg
    18301 ataccagaaa tttggatgga agtcctcatt ttctgtttgg attctcactt ggtaatggat
    18361 ttggatttct tggtcttttc ctaaatagat ctgattgctc ttgatggtat ttccttctat
    18421 ggaaatagat ggagtgaccg ttgtaggact tgtccccaca ctcttgactg aaagtgtacc
    18481 aggctgataa atgaatggtt ctgcgattgg atcatttatt tgtccattgc ttacagttgc
    18541 tgagatctgt acagcttttt tggctaggta ttcagagata tctgttgcat gatcagctga
    18601 ttcatagtaa agatcgcctt tttcatctga tgaaaccatt tgtcgcatac gagactctac
    18661 ttctgctttt gagagaccag ctgccggatc gctttgaagt tggatgccaa gaccatgtat
    18721 ttcgattcct cgttctttga gtgccatcgc ttctccgatg gttgcgataa acgtactgtc
    18781 gattcttctg gatagattgt tttggtcagg tgcatcatag attcttgaga ttagagaagt
    18841 atttcccggc cgatcttgcg tattagaaaa ctgagttccg taataattgc tgcttgattg
    18901 tgcgtgtacc cgctgtactt tataggaaaa tgttggtaca ccatccgtca gcaaaacgat
    18961 cactttttta tgtccatttg gaacggatag catgcttcct gcatctctta gtgctttttg
    19021 tgtaaaagta ccaccatttg tccgtgaagg tgtaatggat tttacttgat ttttcactga
    19081 atcaaatgaa cccatctgta ctgcaccgtt actgtagcta tatccttcgc ttgagtagcc
    19141 gacatatccc atattgattt tgtctgtgat accgctatct gctaaagtat cgacaaaacg
    19201 atcgacacca atctttactt caccgatccg attattgtcg ttcatacttc ctgaccagtc
    19261 tacgaccaat acgagatcaa gaggcgtgat atctttttgt acatttccac gagcattcaa
    19321 ataaacatca aacaatccgg gtgtacttgt ttcctttgca tactttcgta tcgcatagtc
    19381 tgcttgatta ccggttcctc catattcgat atacgaatgg gtccgatcat taggatctcc
    19441 attccaagaa tttataccat cccattgatt tgttccgttt ttattgcctt gatgatcaag
    19501 aacatttgta tttcctgtag gttgccaact ggctttagga taagtgccct gatcatctgt
    19561 tgtatattct gtaggaatcg tttgattaga tggcacactc acagctggca aggataatgt
    19621 aggcaatgta aatgggctga cagaattttc gttttctttt gatgattctt cttctatttc
    19681 gtctggcttt ggtactgaca catctgtatc tttcttattt tctgtctcat caataatgtc
    19741 atattcttta ttatcgacga tcagcttgtt tgcctggctg tggtaaagtg ctggatcgat
    19801 tggaaaactc agatcgatag tttgtttagt gtttgatgag atttccaacg taattttccc
    19861 gctatttacc tgataagcat agccatctcc ttcttttctt tccacatcac ttaagtaatt
    19921 ttcgactaga ccaatttctt tttctatctt ttcagcactg gtgttattcg catttagctt
    19981 gatgttcagt gctttttttc cagactgatc cgaaatgact tcagctgaaa ttgtttctaa
    20041 aactgttgga tcatctgtag tgtctgctat tgcagataat ggcacaaagt ttgataaact
    20101 caatattatc agcatgaaaa taactttcag tttcttccct gtggtcgtca tttttcccct
    20161 cctttttctt tttttctgtt ttgacgtgaa tagtgaaaat tattaaaata aacaggcgtt
    20221 acataatttt atttatttta atatgataat tattatacga agggcgctaa agggaatcac
    20281 accaattttc ataccaattg gtttcattta tgaagttgat tccttcgatt aatgagcgtt
    20341 tcattgaaag aaaagggttg agggagaaaa acatatgatc aggttgttgg aaggaaacta
    20401 tagaaagatg gaaaaaatca ttcgaattct gtccatttct gacaagtgga tgactaaaaa
    20461 agaacttgcc aaacgaattg gtagttcaga atctacattt gtaaggtata tcgaagagat
    20521 caaaatccgc tggggaacgg atgtaacgat tcaatcttca caaaaattgg ggtatcgtct
    20581 tgaacatttg aatgtaccag cttatttaaa tgttctgatg gaaatgtcgc aaagttctac
    20641 aaacagtcgt ttattaaatg agattattgt caatccgggt aaaacgattg agtattattg
    20701 tgaaaaaatt tccatcagcc ggtcttcttt tacacgtaag ttgaaacaat gcaatcaaat
    20761 tttagaaagc tatttattaa aagtgatcgt tgaccaaggt tttcagttaa ctagccttaa
    20821 aagtgagctc cagttgcgta tctttgttac ctttttcttt ttaagttact acggaagacg
    20881 cacgttgcca tttccaatcg aaaaaaagaa gatcaaagaa attatggtca agaataattg
    20941 tcagatgaat ttgtttcagg aagaaagccc atatgagcaa tctttcttta ttatgtattt
    21001 tgttgtgcat ctcatgagag aatcccaagg aactccattt gaatggaaag ctggagactc
    21061 ttatgtacag ttgataaata tcgataaaaa agacttctta atcttgaaaa atctcttagg
    21121 aaaattgaag tacagcagct atataaaagt agtgaactat tttgtcaata cgattttttc
    21181 agaattttcg gagaaagaaa aaaatgaaat cagtgagcag atacgaaaaa atatgtccta
    21241 ctgttcgttt tcatccgctg attgcaaaga aaaaaacgaa gaatatgttg ttcaattatt
    21301 aacgcaaatt tattttttct cccgtatttt cccatacgat acgactcatt tgacgatccg
    21361 tgcaacttac tttgcacagc agatgcaaca ccgccagata cgtctatatg ctggatttcg
    21421 tgcagaactt caattttatt ccactctact gaatcagaat ctgatttatt atttatctag
    21481 ctatatcttt tggatgctta tcggtaatcc tgaaattcac cattatactt ctgataaaaa
    21541 aatactgatt atcagcgact taagtcttcg acatagtcaa tatattgaag aatatatcag
    21601 tgatattctt gctgtgcata aaattcactc tgagacaact gctattactg aagatcagct
    21661 gtcgaaatac aatcttctcg agtatgatct gatcgttacg aatcaaccca ttttgaatgc
    21721 ccatgttcct catatattga ttgatgatag tgtgtcattc gcaaatgaag aagaactcat
    21781 ccgcttattc gagttatgaa taatcaaaaa acagctatgt ataaaccacc ctgataaaaa
    21841 tttcaggaca aatacacata gctgtttctt tttattgtat tccttcagca atccaacgag
    21901 atttatagcg aaagtctttt aacaactctt cttttgtcca attatttgca atgagatccg
    21961 tcaaagtgtt cattacgagg gtcatggaaa agtataggaa actaactgct tcttccgacg
    22021 attgaatgga gaaatgattg attcttaata attccagcca tttttcgttc attggagaat
    22081 cagccgatag ttctctacgt ttggttgtct gctgatcatt tcgctgggtt aacaagtata
    22141 acctttgcca atagatactt tcgtgatcta agtcgcagca ccactctaaa tatttttcga
    22201 tccccaaaaa gaaatgttgt ttgttttcgc taatgaattt taagatatct tgatggacta
    22261 tgtgagaact gcttttgatc gtatacaaat aagcatcttc caaatcttga aaatatttat
    22321 aaaaagcacc gcgcgacatc cccatcttgt caacgatttc tgagactttg acttggctga
    22381 tgtgctgtga gcaaaatatc tccagtaata ggtcatcgat tctctgtttc tttgcgttcg
    22441 ataaattgaa aaaagtttct tttggcatcc acatctctcc tttatctctg attatacgtg
    22501 gatagccctt cattgtcatg aaggattcat tccttctttt tctcttcttc tgtcaagtac
    22561 ccatcttcca ttcgataaac gcgatcactc cattgaatca tacggacatc atgagtaacc
    22621 ataatagtcg ctttttgttt ctcatgagct tctttaacta gtaatttgac gacttcatat
    22681 gcatgatctg tatccaaact agctgttggt tcgtctgcta agatcaagct tggctcatta
    22741 tataaagcgc gagcaattgc cactctctgt ctttctccac cagaaaggtc tttaggataa
    22801 ctatttttaa gtgactggat atctaaagat tgcagcaagg aatcgatttt tgttgaatct
    22861 tctttttttg aagtaacttg gtcaactagt tcaaattgtt cctttaccgt taaaaaagga
    22921 atcaaattag agctttggag aataaatcct atttctttaa agcgtaaatc tgctcgtttt
    22981 ttttctggaa gctcagttag tgagtgaccg ttgatcacta tttctccgga agtcggtgtt
    23041 tgcaatcctc ctgaaatggt aaggaaagta cttttacctg aaccagaagg accaatgatg
    23101 ctgacgaatt ctccttcctt tacataaaaa tcaattgctt tcaaagctga gacctttgta
    23161 tgaccagaac caaatgtttt ggtaactttt ttcatcgtta atattttttt catttttatt
    23221 aacctcctat tgctgtaatt gggtctactt tggttatcgt acgtatcgaa aacaatccgc
    23281 ctagcattgc gacaatgatc aaaatcccac tatatagcaa ccattgtgac caaaaaacag
    23341 caaaaggcat cgcgcttggt aaaatcagac tagtcaaata agcaaacaaa agagcaagca
    23401 gaacgcctac caccccaaca ataaaggact gtgctactaa agatttagcg atgaagaaat
    23461 ttttgatccc ttgtgctttc attactccaa aaatagcagt cttctgaagt gtcatcacgt
    23521 acatgaaaat accgaccacg gcagcaacca ctaagaacaa aaagtatatc atcgcattta
    23581 aggtcatatt ttgggcagaa tagccaggaa tactttcaat gaatgtccca atagttaata
    23641 actgtagacc cttcgatgct ttttctttcg aaattttagc tgaatccttt attacgatcc
    23701 cattgatcgg ctgttcttct tcagaagcaa acggctgttg tccatacttc aaagcagtcc
    23761 acgtatctag ggacgcatag atgacgggag aaactgtgta ataagtttca ggaaagatac
    23821 cgacaatcgt caaaggatcg tcgtaactgc caattttgat cgtatctcca attttatagc
    23881 ctgaatcagc cagattttga gagatgatga tttcattctt tgttttaaat tcatgccctt
    23941 tcgtgagttt tggtaataaa aatgcttctt tcgttgtgcc gaaaactgta acattttctt
    24001 tgttttttcc tttgattgct ccgctgtaca atccgattgg tgctttctct cctccttcaa
    24061 tgtatttcag atctcctctt gttaattgtg aagctgcaaa tacttgattt gcatcctccg
    24121 acaaaacaat ctcttgtgcg tcccaatcat cgattgcttt tttaaactct tctgaaagtc
    24181 cattcgctag tcctgacaac atgaatacga catacgcaat caagaacata ataccgacga
    24241 tcaagccata tcgtaattta gaatagcgaa tttctttgat agctagaaac atttgttttt
    24301 cctccctatg atagattgat agtgacaaaa tgtcacttgt tgaatagtgt aactctaaag
    24361 tgacaatttg tcacttgtgt tgtttttttc ttatcaataa attgtctaag acatctttcg
    24421 ataaaacctt aacatgagtc ataaaaaatt tatttaagcc ttatttatgc tttccatgat
    24481 aaactaaagt gaccaaatca atgaggtggg aaaatgatta aactattagt agtagaagac
    24541 gataaagtat tatccaataa tataaaagaa atactcgctg caatcggcga gataacacaa
    24601 gtttatgatg gtagtgaagg actgttcgag ttggaaagtg gagtttatga tcttgcaatc
    24661 ttggatttga tgatgccgga aatgaatgga tatgaagtgt tagcaaaaat acggaaagaa
    24721 aaaatacaga ctccagtatt gattttgact gcaaaagata gtttggaaga taagattgaa
    24781 ggatttgaac gaggtgcaga tgattatcta acaaaacctt tctatcgaga agaactgatc
    24841 atgcgcgtga aagcattatt gaaacgctcg ctaggtctct ttgatgaaaa tgttctgaag
    24901 tataaggata tcagttgtga tttaaaaagc aatgaagtct tgtataaaga acaagttttg
    24961 cccatacaag gaaaagagtt tgaattgctc gtttattttt tacaaaataa gggaatcatt
    25021 ttgacaaaag agcagatatt tgaccggatc tggggatttg attctgatac gacgattaca
    25081 gttattgaag tttatatgag caacttacga aaacatctgc gaagtactgg attggataaa
    25141 gaaatcaaaa ccttacgtaa tgtcggttat atactgcaaa gggtgtgatg caatgaatgg
    25201 agatattaca cgaaagcaac gtagtcgctt ttttgcgaca aatgtagcag catttgcttt
    25261 tatctttttt cttttgggat tcattacttt acagatatta aaccaatctg catacagaga
    25321 aacagatctg tcattagaga aaataaattt tgattctcga acagtacaat tagagataca
    25381 aagatatcag caaggcgatc cctttttaaa cccgactcaa acggaacaaa acagaacaaa
    25441 catggaacag ccattaatgg aaccatcaaa tgaccgtttt aacactcaag tgatcttgtg
    25501 gtctaaaaat ggtgagatat tgaataaagc agctattggt gggcgtttca accaaatcaa
    25561 agaattaaaa atggatacga aaaatctgaa taccatccaa aatattgaat taaatgaaga
    25621 cagtggctca tctgccctct ctttccgttc gattacgaaa aaagcggaga cgaatcaaga
    25681 tggcgttgcc tacatccaaa ttttagaaaa tacaaatcaa atcaagaatt cattagcgac
    25741 ttttcgcacg attatgattt tgtgtatgat tgttttttgg attctctcta ttggtatcag
    25801 ttattacttg tctagtctaa gcatgcgccc tattattctt tcttggagaa gacaaaaaga
    25861 atttgttgag aatgcttctc atgaattgcg cacaccgtta acgattattc aaaatagttt
    25921 ggaacatctt tttaccaagc ctaatcatac tattattgaa gaatccgaaa gcatcgctca
    25981 agcattgtca gagacgaggc gcctcactgg attgacttcg gatttgctga caatcgctcg
    26041 gaatgattct gatcagcagc tgctttcaaa gcaaatgatc aatacacaag aatatatcga
    26101 acaacttgtt aaaccatttc aagagatggc catcatagat ggaaaagagt ttattctcca
    26161 taacgatgca acattagaag taccaattga tgaaaaaaaa atccatcaag ttttggttat
    26221 tttgctggat aatgcattaa aatatacgaa tccagaagat aagatcattt tggattcatc
    26281 tgttaaccat aaagaatggc tgattcacgt aaagaatact ggcccttcta tcagtgaaga
    26341 agatcaagca aggatttttg atcgatttta tcgggaagat caatctcggg cgaaagagac
    26401 tggcggatac gggcttggct tggcaattgc aaaacaggta atcgaacagc atcatggaca
    26461 gatttctgta tccaattatc ttccacaagg agtcgatttt aaagtccaat tgccgttgaa
    26521 gcctaaaaaa taatgcttat ccctaaaaaa gggctgtgac aaaaatcttg tcacagccct
    26581 tttttgttgc gaataaacgg tgttccagaa gtagcttctt cggaaataag ccgaaatttc
    26641 tttaagctga ccacttctgt cacaacctct ttagcttacg cttacgattt tttattgcgg
    26701 tcttcaatgt gtacatcacc gcttgtgtca atatccaatt cttctcggct aacatcttct
    26761 gtcacttttt caacatcttc atgttcttct ttatgaatcc caacttcttc acgaataact
    26821 gtatgtttgc ttacatctac ttgctcttct ttgattggga tagtaatcgt gtcatcttcc
    26881 aatttttcgt ctgttccctg tgaagattga tctgttactg gtttgcgttc gatgacgatt
    26941 tcttcacgtt tgaccggtac ttctaccgtt tcagtatcgt tgaccacatg tttgtggatg
    27001 tcgacttctc cagttgtcac atcatgtgta tttacatcta atttttcttc ttttagttga
    27061 attgtatcat catctagatt tggtttgcga tcgatgtctt cagtcttgct tgtacccatt
    27121 gtatcagtct cgccaggtac gtccatcgga tcagttcctg gtgtcatatt caatggatcc
    27181 gttgtatttt gaaagcctgt cgtgccacct acgtccattg gatctgttcc agtgccagtt
    27241 tctaatccag tccccattgt gcttgctggg ctaacagtat cttgtgtatc cattgtatct
    27301 tctgttgttt ctggctgacg atagcctttt actgtgatga caaattttcc atcagaaata
    27361 tctcctctat attgatataa aggatcattt tctggtgtag ccgtctcaga atcataggta
    27421 tcaaaagaaa aagtatcttt tattttttcc cataaagaac ggtcttcttc accatccatc
    27481 cgattttcat ctgtttcaac atctatacca gacaatccgt caaatcctag tctagatgct
    27541 gcttcatcag tagtataaat aacgatatca tctcgttcat acccttcgtt acgcaaccgt
    27601 tcaattactg ataatgcttc ttccctcgta ctatagcttc cgatgaccgt taaatttttc
    27661 tcttccatgt aaattcctcc tagcaaatag atttatgatt ggtatattgt tacttataaa
    27721 tttattctat aggagacgat tgttttctac aaatatttag attggaattt gattaaaaca
    27781 atttaattga aatttgaaaa catttatgtt aatgaaagtc atattctaat aaaatgtcat
    27841 ttgtttctat atgaattttt atgaaaaatt tcatataatt tccactcgtt gtgatggttt
    27901 aatttatcca atccatcaat aagcaaacaa cttatataaa aaaggattaa ttctcaatat
    27961 aattgctcct accatagaat aaatcttcat gtaaagtaat gaagtaccaa ctatttctaa
    28021 tacttcttta agaattatcc caacaatatc gttagacaaa ccattattga ataaatggag
    28081 agccaatcga ttctgaaaaa atgttttcta atttttcgga aataaaagaa agagcctcct
    28141 gccattagaa cgaaccaaat ccatttcatt gctcatgaag taaccacata gctaaaaaga
    28201 ggataaatca attaaaagat caaaagaatg actgcactaa taaaataact aaaaatgtat
    28261 cttcttactt catatctttt agctagtact tagttaaaaa tttgcttcct aagatcaaga
    28321 caaactgtct atcttcattt gatgaaatgc ggtaaaatat agaaaatgaa cactattgga
    28381 gggaatttgt aatggacagc aaaactagaa aacaactgca agaaaagtta aagcaacgta
    28441 cacacttgct cccttccgtc tttcaacagt ttttagaaga aaatcgttct tccctttcat
    28501 taggcagtcg ctatgaatac gcaaaagatt ttcatctgtt tactgattat ttgagaaaag
    28561 tattttctga agaagtggat gatcagctga ttatccattt agaaaaacaa gattatcaga
    28621 atttcttagc taaaatctat cgacatactc gttcgttcca aactacagcg aaccaagaaa
    28681 cggagcaatt gtttgaaaat agctattacg ggatttcaag gaagcaatat tcattaaatc
    28741 attttttacg atttttgtat caaaaaggac tgattgaaga agagatttct cttttgggta
    28801 caagtttacc ggaaacaacc aaagcagctc cgcgatatat cgatgcagaa cttttcaaag
    28861 attttgagca tttgtttatc ccgattgaag gctttcaaac aagcagagaa gctagctttg
    28921 aagaaaagaa tagaccgagg aaccttgcaa ttactgccat tcttctgtat tgcggattaa
    28981 agatccatga agtagtagaa cttaaacgta aagatgtatc tttgaccaag cagattctta
    29041 atctgaatag aaaagaaaac agactgaata aagtaccaat accaaaagaa gcgatgccat
    29101 ttctggagaa ctattttaag ttaacagcac aaaatacgga cgaaaattct tttgtttttc
    29161 gttcctcaca tgatcagcaa atttctcctc ggactattcg ccaaattttg agcaaaatta
    29221 cagtatataa aaacgtatcg ccagaactat gcagaaaaac atatcgttat tatgcagaac
    29281 tatatttaga tgatgctgac gcggtaaatt atttatgtgg taatcgatac ctctcatccc
    29341 attcttttca agatatctgg gaaaagatgg ttgatttttc ttatcacgaa ttagcggcat
    29401 tcgttcgggt ttcttccata taaaacaaat gtatgatctt tttttcacaa taaactatgt
    29461 tattatataa aggattatgt tacgattatc ttgaatttgt atattactta acaaaagata
    29521 atggagtgtg aatgatggag accataacgt attccccttt gaacttattt acgaattttc
    29581 aagaagctgc agctaacttt tccgagaccc cgattatctt tgatgagcca ctttcagctt
    29641 tcccagaatt aggacttgaa acaacttaca aaaaatgctg cgaaagtgtg ttgaaacggg
    29701 cttatcaatt ggctcatctt ggagtgaaat ccggagacaa agttattatt tataagagtt
    29761 ccgcttttga cacatacctt ctagctgttt ctgtgtctta ccttggtgca gtgcctgtca
    29821 tgacttctta tcatctacca acaacaacaa tggaagtatt tatcgatcgt ttagaagacc
    29881 ctttcatttt atttgatgat gagacaaaaa agcgagtgag agaaatttca aatggaacaa
    29941 aatcaaaaca gattcctatt ctttatttaa tggaacaacc tgctttgtct gtttctcaat
    30001 catttttaga caaaaatgaa atttcttata tgacccatac ttctggtaca actggtatcc
    30061 ctaaacttat ttgtcattct gctcattcta tgggatggcg gacgaaatgg caaaaaacag
    30121 ttttcacaaa aatttcggaa aagaaattaa tcgcttttca catctctcca gtgcattcca
    30181 gattcaatat tggtatctct tctttgatgt ccatgggatt tcctatgatg cctttagcaa
    30241 acgcacaaag ttcaaaagtt gttcatatgt tggaagctca ccggccaatt gcactagaga
    30301 cccatccgaa taactttgtt cagtggcgtt ttactgctaa ggagcatcca gaagcttttt
    30361 cgggtatccg atactatcac tccacttttg atgctataaa caaccagaca atgaagacat
    30421 ttttgcgaac gtcgatggca caggatgcgg tatttcttca agtatatggc caaagtgaat
    30481 gcggaccaat gattcttaag gctcatacac tagagagttt gaaaacaagt gatgcaagag
    30541 atatgggcgt tggactggaa gacatgacaa gcgctcggat tacagattca gttgggaatg
    30601 tcctacctga aaacacagat ggtcatatac aattactgtc taaaggacgg gctttgactt
    30661 attacaagga agatgcacgg ttccaggaaa atgtttatgg cgattggtgg gatagcggag
    30721 attacggttt tatggataag catggccacc tatttttgaa agatcggcaa gtggatttga
    30781 tcgaaacgat caatagtaca ttagctattg aagacttttt attagattcg ttagattttt
    30841 tggcagaagt agtgattgta agggataaaa atcattctcc acaaccgatt attgctttag
    30901 ctcctgataa agaaatggat tggaatcgat ggtgggagca agtccatgaa cttcctcgct
    30961 tgaacgtccc tatcatccgt gattttgatg caatcccgcg aacagctaca atgaaagtgc
    31021 aacggcttca aatcgagaaa gaattaaaat cacaaaagtc ctaactattt aaggaaagaa
    31081 agatccattt tggtctttct tttctttttg tttttttaaa ttggatgaga taaaaatatg
    31141 ccaattggct gtttttcttt tttgaaaatt tcgttatact cttattcatg gaggaaaatg
    31201 ttatgaaaag aatcttgaca attggcggtt ctgatccatt tgctggtgga ggaatccaaa
    31261 cagatttaaa aacttttgag aattttggtc tattcggact aagcgccttg acttctatag
    31321 gggcactaga tattaatgag cgatttatgc tggaatctat atctttggaa ttgcttaaac
    31381 gacagttagt ttcgattgat aaaatgactt cattagatgg tatcaaaatt gggttattga
    31441 attcaagtga agcaatcatg gttgttcgag acttcataaa aacaaaacaa aatatcccaa
    31501 tcattttaga ccctgttttg gcattcaaag aaactgaagc tgtgatcaat caattgtata
    31561 tttcgcagct catcgaagaa ctttttccac ttgttacgat gattacgcct aatttggcag
    31621 aagctgcttt actttctggt caagctcttc cctcttcgtt aacagaaatg atccatctcg
    31681 ctaaagaact cactgcttca ggaacaaaaa acattgtgat caaaggcggc acaaggatgg
    31741 ctggagatga agctgtggat cttctctatt caattgatca ttatgaggta ttttctcata
    31801 aaaaaatcga cactacaaca gtaaacgggg ccggttgtgc cttttcttct gcaataacag
    31861 cgaatcttgt gttaggggca gattttacca aagcagtagc agaaagtaaa aaatttgttg
    31921 cccattgtat ttctaatggc gtgctgatga acgatcatac cggaagtgtc tggtctggcg
    31981 gtattcaaaa aggaggaatg agaaatgaaa actaagcaat taacgatcta tgctatgctg
    32041 attgcgttga cagttgccct ttcattaaca attttgatcc ctgtaccggc tacgaatgga
    32101 tttgtgacat tatgcgaggc cgggatctat accgctgctt ttctttatgg tccaacgggt
    32161 ggattagttg ttggtgcttc cagtggtcta ttgatcgatt tgatttcagg atatccgcag
    32221 tgggcagttt tttcattagt gatccacggg ttacaaggat atgttgctgg ttctttcagc
    32281 aaatcttcta ctcgtatgtg gggattaggg cttttattag gaagcttagt tatgatcatc
    32341 ggctatggta tcgccggttg gattttatat gattggccgg ctggagtggc ttctatacca
    32401 ggaaacatct tccaaaatct gattggaatg ggacttgcct ttcctataac tgcaagtctt
    32461 caacgtattc aagcacggaa gcaatattaa ttgaaaaaga aggttttaga aaatgaagtt
    32521 gtcagaaaaa gaattaaaag accaattgac tgaaatagtt aatgacatat tggctgaggc
    32581 acacttgaaa aaaggtgatc tttttgtttt aggatgcagc acaagcgaag tcgttggagg
    32641 acatattgga aaaaattcca gtgcggaagt tggtcaatgg atcatccgta cgttaaaaga
    32701 gttgttggat cctaaagaaa ttgcgctagc tgtccaaggt tgtgaacatt tgaaccgagc
    32761 attagtagta gaacgaacag tggcagaagc aaaaaatttc gaaattgtct ctgttgttcc
    32821 tgcactacat gctggaggtg cttgttctgt tgctgctttt gaccagttca atgatcctgt
    32881 cgaagtggaa cacgtagttg gacaagccgg tatcgatatc ggtgacacat caatcggtat
    32941 gcatatcaag catgtccaag tacctgtcag acctcgtttg aaaacccttg gacaagctca
    33001 cgtgacagcc cttcgctctc gtcctaaata tatcggtggt cctagagcga attattgaaa
    33061 tgagtaattg acagagcaat ctgaaaaact tcctcgcaaa gtccaactaa tgaactttaa
    33121 cgaggaagtt ttacttgttt gttttatata accgagcttc ataaggtgct agtatgcctt
    33181 tagcttcaaa ttctccgcta gaggaatttg acaagagtaa tgtccaggac tgatctgctt
    33241 cttttggaag ttcatagtca gcaagtgatt ctgttaagtt taccataata agatattgtt
    33301 catcttctaa atatctttca tagacataaa gttgtggatg ttcatgaaga tattctttat
    33361 aagaaccgta tatcagacct ttattggttt gtctaatgcg gatcatgttc ttatagaatt
    33421 gcaggaccga ctctggttca tattcttgtt gttctacatt gatcgttttg gtatttggat
    33481 tagtgatcag ccaaggttca tgtgtagaaa aaccagcata ttgttccgaa gtccattgca
    33541 tgggtgtcct gctgttgtct ctcgttgttt cacgaatgat atccaatgct tcttctcttg
    33601 tcttaccttc cgcaagcagc cttttataaa gtcttttcga atctactgca tcaacttgat
    33661 cgatcgattc aaagggcata ttcgtcattc cgatttcctg tccttgataa ataaatggcg
    33721 taccttgcaa aaagaaatac atcatagcga ttgcttttgc agacatagcc caaaaatcgg
    33781 gacgagtatc tccaaacact gaaacggcac gaggaacatc atggttttcc atatataaag
    33841 cgttccatcc gataccatcc aatgaagttt gccaagcgct taatgcatgt ttcaatttaa
    33901 ggacatctaa ttgcccttct ttttcttgct gccagatatg gatatgatca aattcgaata
    33961 tcatatcgaa ataaccgtct ttccctaccc attgtggtcc ttcttctgca gtgacaccac
    34021 ttgcttctcc gacagtcatg atatcgtact cttcaaaaac atgtttcaaa tcggtcaaat
    34081 gttcttcgat tcctgagaca ttttgaaagg gtgagaaagg gttctctgtg gcttttactg
    34141 aatattcatc tttttttaca tgagaaatcg catctaaacg aaatccatct atccctttat
    34201 ccaaccacca tcggatcata ttgaatagtt cttctttcaa tttctcgctt tcccaattta
    34261 agtctggttg ttcttttgca aaaacatgca gataatactg tccagtttca tgagaatact
    34321 cccaagcaga accgccgaaa atcgatgtcc aattattcgg ttctttgcca tctaccccgt
    34381 cgacccaaat gtaataatcg cgatacgggc tttctttaga ttttttcgat tcttcaaacc
    34441 attgatgctg atccgaacta tggttgatga caagatccat aatcactttg atccctaatt
    34501 catgtgcttc ttttagcaat aaatcaaaat cttccatcgt tccgaatctc tcttctatat
    34561 cttgatagtc acttacgtca taaccattat cgacaaatgg agattgatag atcggattga
    34621 tccagataaa gccgatacca agatctttca aataattaag tttttcacgg atcccattga
    34681 tgtctccttg accatcgcca ttgctatctt tgaaactagc aggatagatt tgatagccta
    34741 ctgcttcttt ccaccatttt tgttcttgta tcatatcgtt tcactccttt aacggtccct
    34801 cttgattata accgattcta ttttagaagt atccttgtta ccggtaacta gaaacgaaaa
    34861 gctcaacgaa tctgctaagt gcgttcgctg agctttattt tatcatagat agcgggcaat
    34921 cagttgaatc atttgcttgg gaaattcatt caagtcagta atatctacaa aacgactttc
    34981 accgtagatc gacttgattt cttctttgtc ttggccaata gctgcggaga caaataggat
    35041 tccttgacgt tcatattctt tcaaaacaga ctggatatct tcttttgctt ttttgcctgt
    35101 ataatccggc aacgcttttg gctgaccgtc actaatatta agcaacagtt tggttgttgc
    35161 ggattgtctg tttaattttt ctgctgctaa acgaagtgca gcaccatcac gattgttttg
    35221 ccttggtttc atagtgacta atttttcatt taaccattga aatccgtctt caaattcttt
    35281 ataagaaaac aaagaagtct tttctcttga cgaacggtct gctgtatctc cgtaaatcaa
    35341 caatgggata tcgactttct tagaaaattc ggcaatggca atcgctgctt tttttgccgc
    35401 ttcgatccga tcatcccgta tcattgaccc agattcatcg atccgtacag ctaccgctaa
    35461 agaaggttgc tcatgtggcg ggttcttttt gtcaaaattt ctcaaatcac catatgctac
    35521 tttgcttgca ttgaaccgct gaccttcgta ttttcctttt tggtacgtct ctgtttgttc
    35581 attatccagt aactctaaaa tttgccggct aagtgattct actattggca ttacttgcat
    35641 gtgtagctca cgtgcttccg gcgtttttcc ttggaaagct ggtcggtgaa cgatgagacc
    35701 ttctttttca tgaatcgttt ccttcatgat ttgtcgcgct tctttattca gttgcttctt
    35761 gatttcctta tcatgtgctt ccatgtcaga agcagacatt tcttgagaat catctcctgt
    35821 ttgttgttct ttttgatttt ttccttcgat atcgctactg cgttcttttt gcatttgagg
    35881 aggaatctcg tttttttcta ttgtttcttc ttgtttctct tctccctcat ttggttcttc
    35941 agcagacaac gatttctttt catctaagcg cagaatttct tcagcatttt cagtgatgtt
    36001 tcgttcattt gcttcttcgg cgctaatggt tctttctttt gtatgctcag attgaggatg
    36061 accccatttt tcttttctca atatgtcctg aagttccagt agcaatccac tgtcagacaa
    36121 agcttgttca agaattgcca attcatctga atcagtggat aatttataca aaacttttgg
    36181 ataaatcgac tggagtaaat cttgtccttg cttataggta tttgcccaat aaaaataaga
    36241 acgcattcct gctacacctt tgatggcgtt agcttttgca gtttcatcca acaaacggat
    36301 aatctctgcc atctttaaga gaagttcttg tttttggatc atagttttcg ccatcgcacg
    36361 ttctgccatg acttctaagg caggaagatc cattttttcc gcatgttgca tccgatctct
    36421 taaagattca tttaatgggc gatttccttg gtaatttcga tttgtagtga tcacaatgac
    36481 gcaatctgga tggcgacgaa tgattcctgt cggtaaattc aacatcccat ttggttcgag
    36541 cgctgagtta agtgccacaa gaacagaggc atctcggatg acagtcggct cttgaatctc
    36601 taataaatag cctttttcaa tcgctcgaac gatttcagaa gggtaatagc tgtaatggat
    36661 tgcatctgga taatcagctt cgatcctttg aactagctct gctaattttt ctttagcaga
    36721 aaaaggatca atgccgtaat ggttctggat cagtgtcagt acagcttcaa gtgtatctgt
    36781 ttggtaaata gcttctagta attcttggtc ttgttcctta ttagaatcta ctacaggtaa
    36841 taaggctccg aaaacatctg atttgtccat atctgcaaaa caggtcactt ttgtataggg
    36901 aagttgcaga tcagcagata gtgctttggc taattgtgtc ttccccgacc cggcatctcc
    36961 ttccaaaagt acgttactga ttttcatttc aggatcttgc caattccctt tgatttcgga
    37021 ggcgatccgc cattcttctg cacttgtccg atggcttttc ggtttcttcc agatcatttg
    37081 cttttctagc cgtgaaaagg ttcggttttc tgttagtgag aaattctcca tgcttctctt
    37141 ccccttttga tcatctctgt ctattaatat cctagctcat ctaaaagacg ttttaaaata
    37201 cggaatcgtt ccccaatcag ttctccgtct ttttgtagtg tatcgtaata caaaatgatg
    37261 tcttcatcga tctttggatt tttggtatct acttctaccg tcattcggtt cccttgtaat
    37321 ccaatgatgt ctacgactgg attttctgga tcataaaatt tttcataacg ataagcatct
    37381 tggaagtata aacttccttg cgcagctaag atcgctaaat ctaaaatacg tgtgattggt
    37441 aattcttccg actgtcgtga ccatttttct ccagtatgtc gccatacctt gcctgagata
    37501 tcgacttttc cacgatcatt ccactgagca aggcccagtg aaagcccttt tgcatccata
    37561 tttaatgcat ttcttccatc aactttatca tagtcttcta caacgattac tggtttatgt
    37621 tttaaatttg ttggtatttc cattatttta actcctcatc cttttatttt tagtaaataa
    37681 gtaaattagt agtttactca tttactaaac tatatgcgac attatagagg attgaagtga
    37741 ggattgcaaa gaaaaagagc ttcattgata gaaataaaaa caccatctcg ttcttttaca
    37801 cgctcattag taacatgtag aaagtccaag atgatgttgg aat
//
LOCUS       DAJSBL010000025        37675 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.025, whole genome shotgun sequence.
ACCESSION   DAJSBL010000025 DAJSBL010000000
VERSION     DAJSBL010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37675
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.025"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            72..2237
                     /gene="acm"
                     /locus_tag="ONT85_001470"
     CDS             72..2237
                     /gene="acm"
                     /locus_tag="ONT85_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HCR2378452.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIGLLIVTICAGIFLQKYKKV"
     gene            complement(2509..2994)
                     /locus_tag="ONT85_001471"
     CDS             complement(2509..2994)
                     /locus_tag="ONT85_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HCR2378453.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     misc_binding    3084..3429
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            3474..4115
                     /locus_tag="ONT85_001472"
     CDS             3474..4115
                     /locus_tag="ONT85_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HCR2378454.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIIMKQVEGKLNYYR"
     gene            4134..4769
                     /locus_tag="ONT85_001473"
     CDS             4134..4769
                     /locus_tag="ONT85_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HCR2378455.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            4787..5602
                     /locus_tag="ONT85_001474"
     CDS             4787..5602
                     /locus_tag="ONT85_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR2378456.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            5921..7357
                     /locus_tag="ONT85_001475"
     CDS             5921..7357
                     /locus_tag="ONT85_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HCR2378457.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            7546..9993
                     /locus_tag="ONT85_001476"
     CDS             7546..9993
                     /locus_tag="ONT85_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HCR2378458.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            10223..11290
                     /gene="rlmN"
                     /locus_tag="ONT85_001477"
     CDS             10223..11290
                     /gene="rlmN"
                     /locus_tag="ONT85_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HCR2378459.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(11483..12586)
                     /locus_tag="ONT85_001478"
     CDS             complement(11483..12586)
                     /locus_tag="ONT85_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HCR2378460.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            13139..13687
                     /gene="aac(6')"
                     /locus_tag="ONT85_001479"
     CDS             13139..13687
                     /gene="aac(6')"
                     /locus_tag="ONT85_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HCR2378461.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
     gene            14233..15894
                     /locus_tag="ONT85_001480"
     CDS             14233..15894
                     /locus_tag="ONT85_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR2378462.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(15964..16311)
                     /locus_tag="ONT85_001481"
     CDS             complement(15964..16311)
                     /locus_tag="ONT85_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HCR2378463.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            16395..17336
                     /locus_tag="ONT85_001482"
     CDS             16395..17336
                     /locus_tag="ONT85_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR2378464.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            17337..18380
                     /locus_tag="ONT85_001483"
     CDS             17337..18380
                     /locus_tag="ONT85_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR2378465.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            18386..19456
                     /locus_tag="ONT85_001484"
     CDS             18386..19456
                     /locus_tag="ONT85_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR2378466.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            19446..20399
                     /locus_tag="ONT85_001485"
     CDS             19446..20399
                     /locus_tag="ONT85_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR2378467.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            20411..21421
                     /locus_tag="ONT85_001486"
     CDS             20411..21421
                     /locus_tag="ONT85_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR2378468.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(21706..22527)
                     /locus_tag="ONT85_001487"
     CDS             complement(21706..22527)
                     /locus_tag="ONT85_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HCR2378469.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(22624..22902)
                     /locus_tag="ONT85_001488"
     CDS             complement(22624..22902)
                     /locus_tag="ONT85_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2378470.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            23170..25179
                     /gene="metG"
                     /locus_tag="ONT85_001489"
     CDS             23170..25179
                     /gene="metG"
                     /locus_tag="ONT85_001489"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HCR2378471.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(25658..26245)
                     /locus_tag="ONT85_001490"
     CDS             complement(25658..26245)
                     /locus_tag="ONT85_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2378472.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            26420..27742
                     /locus_tag="ONT85_001491"
     CDS             26420..27742
                     /locus_tag="ONT85_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR2378473.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            27897..28673
                     /locus_tag="ONT85_001492"
     CDS             27897..28673
                     /locus_tag="ONT85_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HCR2378474.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            28666..29235
                     /gene="rnmV"
                     /locus_tag="ONT85_001493"
     CDS             28666..29235
                     /gene="rnmV"
                     /locus_tag="ONT85_001493"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HCR2378475.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            29228..30112
                     /gene="rsmA"
                     /locus_tag="ONT85_001494"
     CDS             29228..30112
                     /gene="rsmA"
                     /locus_tag="ONT85_001494"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HCR2378476.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            30563..31192
                     /locus_tag="ONT85_001495"
     CDS             30563..31192
                     /locus_tag="ONT85_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2378477.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(31316..32425)
                     /gene="ugpC"
                     /locus_tag="ONT85_001496"
     CDS             complement(31316..32425)
                     /gene="ugpC"
                     /locus_tag="ONT85_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HCR2378478.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            32634..33056
                     /gene="mgsA"
                     /locus_tag="ONT85_001497"
     CDS             32634..33056
                     /gene="mgsA"
                     /locus_tag="ONT85_001497"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HCR2378479.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     gene            33379..33915
                     /locus_tag="ONT85_001498"
     CDS             33379..33915
                     /locus_tag="ONT85_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HCR2378480.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(33955..35718)
                     /locus_tag="ONT85_001499"
     CDS             complement(33955..35718)
                     /locus_tag="ONT85_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR2378481.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(35721..37436)
                     /locus_tag="ONT85_001500"
     CDS             complement(35721..37436)
                     /locus_tag="ONT85_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR2378482.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
BASE COUNT        12426 a         6649 c         7761 g        10839 t
ORIGIN      
        1 attgaagaaa ggatttttga gagatgatat agtaggtttt atttttagtt aaaatagggg
       61 agatagaaaa aatgaaaaag tgtagaaata ttattttatc aatgttattt attattacta
      121 atataacatc actgattcct gttcatgtct atgcggatgc aggcagagat atcagcagta
      181 atgtcacttc gttgaccgtg gacccaacga atattacaga tggaggaaac ataaaagtaa
      241 aattctcgtt tgacgaaaaa aaacaaaata tccaaccagg tgattatctt tggatcaatt
      301 ggccaagtga aggaaatatt cgcggtgaag gattccaaaa agaaatccca ttaatgattg
      361 aaaataaaaa tgttggaact ttaacggtca gaaaagattc tgcgcaggtt gtgtttaatg
      421 aaaatattaa aaacctagac tcagtagaag gttgggggca atttgagata caggccagaa
      481 acgtaaccga taccggtgaa gaaaatacgg gaccattcac cgtgacgagc ggtgataaaa
      541 cagctactgt aaatgtgaca aagccagctt ctggttcttc ttctagcgtt ttttattata
      601 aaacaggaga catgctacct gaagatacta agcacattcg atggtttttg aatattaaca
      661 ataacggcac ttatgttgaa caaccagtta aaatcagtga tgaaatccaa agtggacaaa
      721 gattagatcc aagtacgttt gaaattaatc aaatacactt aggtgaacag aaggtttata
      781 gaggagaaga gggaatccaa caatttttac aagatttccc cagcgctaca tttaattttt
      841 cagtaactga taattacatt gaaataacta taccaaaaaa ctttgtcaat cttaggaaaa
      901 taatggtgag ttataagacg atcatagaaa acccagaaca aataaatttt gagaatcatt
      961 cggaagcttg gtttaaagaa tttaataaac ctgctgttga tggagaaagt ttcaatcata
     1021 cagttaaaaa tatttcagct tctggtggag tcaacggtac agttagaggc gaattgaaga
     1081 ttttcaaata tatcaatgac acagagattg gtatcccaaa tgtgactttc gaattaagac
     1141 gagcagatga acagcctatt caaggacagt ctagtatcct gttgacttcg aatgaacagg
     1201 gagaagctag tatcaaaggc ctgcaagtag gagactatgt tgtaaaagaa aaagaagcac
     1261 ccaattggat tgattttgat ccactaagtt caaatgaact caaattctcc ataaacgaaa
     1321 atgatacaga gggagtcagt ttacctattt ataacaagaa aaaggttacg aacatcacag
     1381 caactaaaat ttggaacggt ggaaccactc caagaccgag tatttatttt aaattattca
     1441 gagcaagtac aaacaattgg gaaccagttc ctgatgcaga aacaaaacga ttagacaatg
     1501 gaataacttc agtgacttgg gaagatatcc aacagtatga tgatagtgga aacgaatata
     1561 cgtttaaagt gcaagaagta gatcaaaacg gaaatgatta tgtcccttca ggttatcaaa
     1621 aaattgaaaa cgggctagtc gttacaaatg agaataaaga agtcatttca gtctcaggac
     1681 aaaaaacatg ggaagacaat gagaaccaag acggcaaacg accgacgacg atcacagtaa
     1741 atctattagc cgacggtcaa ccgatccaac acaaagaagt aagtgaaaaa gatgattggc
     1801 gctatcgttt cacaaatctg ccaaaataca gagacgggca agaaatcatc tatactgtaa
     1861 cggaagacaa cgtgcccgaa tacagtacga cgattgacgg gtataacatc aaaaacagtt
     1921 atacaccgga aaaaacaaat atatctatac caggtagcca tataacaccg tggaaaaatt
     1981 ttccagagaa gacagagaga gagaaaatta gaaatctatt ctctcaaaaa ttgcaaatat
     2041 ttgctcatac aggagaatcc agcgaacaaa gaagcaatta tattagtaag agagctgtac
     2101 ctaaacagat aacaaataaa tcaaaaagca ttcttcctaa aacaagtagt aaaaaatcta
     2161 gttttgctgt aatcattggt ttattgatag taactatctg tgctgggata ttcttacaaa
     2221 aatataaaaa agtttagtta gaatataata ttttttgtta aatatgactg aaatcaaaag
     2281 aataaaattc cgatttataa tcgaaaaaag aagcattttt atatatgctt cttttttcgt
     2341 agaatcaata aatatggatt tggctaacta tggaagaata ctcgtattag ttttcagcgt
     2401 tcatactagt tgttacattg tttgattggc caaaagaatt tctgatatat tttgtgctaa
     2461 ataaagaaaa agacgcttga attgggaaat aagaaaacta ctctatcatt acaaattttc
     2521 ataaactggc agatgttcta agtaaacaag tatcttttca aagtcggaaa aatccataat
     2581 caacgtagtg gtattgatat ttggatgaaa gcctacggta tcttgacgat caatagcagc
     2641 atcgatcacg acttggattt ttttctcttt gtcgtgcatc aatgctaatg gagtcactgt
     2701 acctgctgga acgcctagga ggtcaggaag ctgttcttca gaaacaaaag ataagcgctt
     2761 ttcttttaat tgttcagcta gttgttttaa atcggcgtgc ttttcatcag gcaagatgac
     2821 aagatagatt tgttttcctt ttcttgattt caataataga ttttttactt gcggaccagg
     2881 tagggaaaaa gggacatttt tgactgacgt aatcgcagga tgatcgactt tttgaaaaga
     2941 gatacccagt ttttttagta gttcataagc ttcttgttcg gttgcacgac tcatcgcgca
     3001 tcaccctcct ttttttatta gtgtatcata aaaaagagtt gacaaatgaa aaataatcat
     3061 gtaaattaca cgtaaagtta aaacaaatga catcgaaaag aagagtagcc gttaggtgtt
     3121 tttagagagt ctctggttgg tggaaagagg cagcagttaa ccgtgaaaca catcttggag
     3181 tcgattttct gaagcgtata gtaggaaaat ccggggagcc cgttacagct gcggtcgttg
     3241 ctagatgacc agataaggct ggcttttccg tgaggagtcg gtaaaaaaag gtggaaccac
     3301 gtgattaacg tcctttgatc ttcggatcaa aggacttttt gttttcatct gaacggcaac
     3361 gactgtgtga ggttttttct gcaaggaaaa aataaacaga ggtggaacca cgtataatac
     3421 gtcctcttgg catttgccaa gaggattttt ttattttaaa ggaggaaaag aagatggagt
     3481 atttgattga agttatgccg caattattag taggagcact cacaacattg aaattatttt
     3541 tatttacctt gattggctca ttaccattag ggattgtttt agcattagga ttgtctttgc
     3601 attttaagcc gctacgctac ttgcttcaaa catatgtatg ggttatgcgg ggaacaccgc
     3661 tgttgttgca actgattttt attttttatg ggttgccgac gattgggatc gtttttgatc
     3721 gtttcgaagc agcactcatt gcctttatct taaactatgc agcttatttt gcagagatct
     3781 ttcgtggagg gatacaatcg attcctcaag gtcaatatga agcagcgcaa gttttgcaat
     3841 taagtccctt tcaaacggtg acgaaaatcg tcatcccaca agtgacaaag attgttttac
     3901 cttctgtagg gaatgaagtt atcaacttgg tcaaagattc ttccttgatc tatatattag
     3961 gtttaggtga cgtaatgcgt gctgggaaaa tcgcgatgga acgcgacgcg actttactgc
     4021 ctttgctagg cgtggcaatc atctatctta cattgactgg tattctaaca atcataatga
     4081 aacaagttga aggaaaatta aattattacc gctaactgat aggaggaaga aatatgattg
     4141 aattgaagaa agtgaacaaa gcatttggtg aaaaaaaaat aatcaaagat ttgaatctaa
     4201 tcattccaga tgggaaaaca ttagctatcg taggtccttc cggtggcggt aaaaccacgc
     4261 ttttacgtat cttggctggc ttagaaagtt ctgacagcgg agaattttat ttagacggtg
     4321 taccgttcaa cccattcgag cttaaaatga aagaacaagt tgtaggagtc gtattccaag
     4381 acttccagct atttccacat ctgaatattt ttgaaaacat taccttagca ccaaaaatgg
     4441 tattgaaaca gacaaaagaa gaatacacca aaaaagcaga agaattagcg gcttcattag
     4501 ggattagtga tttattgaat atctatcctt atcagctttc tggcggacaa aaacagcggg
     4561 tagcgattgc cagagcattg gctatgaatc cacgggtatt ggcttatgat gaaccaacaa
     4621 gtgctttaga tcctgaatta cgccagcaag tcgagcgttt gatcctatca ttgaaagaag
     4681 atggggtaac ccaaattatt gtcacccatg atttagtatt tgcagaaaat gtcggtgatc
     4741 aattactaaa agtttcccca attcattaag aaaggagaag atagagatga aaaaaaccag
     4801 ttttctattc gctttgatcg caggtcttct tctttttgca ggctgttcga acaaaaaaac
     4861 ttcagcagat aattgggcga ctatcaacga agagaaaaaa atcgttatag gtttagacga
     4921 tacatttgtt cctatgggat ttagagataa agacgggaag ctcacaggat ttgatattga
     4981 tttagccaaa gcagtctttg aacaatatgg gattacggta gactttcagc ccattgactg
     5041 gtcaatgaag gaatttgaac tgaacaatgg aacgatcgat ttgatatgga acggctattc
     5101 caaaacgccg gcaagagaaa agaaagtgca atttacaaaa ccttatatgg aaaacgatca
     5161 agtgctgatc acacctaaag aatcaggaat cacaaacttt ggacaaatga ataataagag
     5221 attaggtgcg caaaatgggt catctggtta tgatgtattt acgaaacaac cagaagtatt
     5281 aaaagagata gtcaaaaacc aagatgctgt tttgtatgat agttttaatg aagcattgat
     5341 cgatctgaaa agcagacggg tcgatggtct gctgatggat aaagtatacg cagactatta
     5401 tttgaaacaa agaaacgaac tagaagactt caacataaca aaaggttctt atgagagtga
     5461 ggattttgca gttggtgcac gcaagtcaga tagtacatta gtgaaaaaaa tcaatgaagc
     5521 cctagatcaa ctagagaaaa caggtgaatt ccaaaaaata tccgataaat ggtttggcga
     5581 agatgttacg ccgagaaatt aacaaatagg ggactgtgac aaaagacgtg tcacagtccc
     5641 tttttccgaa taaacagtgt tctcctgcca actccttgtg actcggagct gaatggcagg
     5701 ctcacaacct cttggtaaac ggtgttcaaa agccagtttt tcggtattaa gccaaattta
     5761 tgcaaaaatg tagaaagcca ttttcacaaa atttttctta ctactcgaaa ctaagcgact
     5821 ttttcacaac ctcctttaca taattttatt ttgttgtgga aggggtttcg tgttattcta
     5881 aaagaggaac gattgaaggt tataggaaag gaagagatga atggaacaca aagaacttac
     5941 ctcatttcca aaagattttc tgtggggctc tgcttctgca gcgtatcaag tagaaggagc
     6001 ttggcaagaa gatgggaaag gagaatcagt ctgggatcga tttgtacgga tacctggaaa
     6061 gacatttaaa ggaacgaacg gtgatttggc agtagaccac tatcatcgtt ataaagaaga
     6121 tattgcttta atgaaagaac aaggtctgaa agcttaccgc ttctctgtag cttggacacg
     6181 gatttttcct aatggacgag gagaagtcaa tcaagctggg ctcgcttttt atgagagatt
     6241 gatcgacgaa ttgattgaaa acgatattga acctatgttg actttatatc attgggatct
     6301 tccacaagca ttgcaagatg aatataatgg ttgggaatca cgtcagatca ttgaagattt
     6361 cactcattat gcagaaacat tatttgaggc attccgcggg aaagtccatt actggattag
     6421 tttgaatgaa caaaatatct tcacttcatt aggttatttg ttagcagcac atccgccagg
     6481 tgtgacagac ccgaaacgaa tgtatgaagt taaccatatt gctaatttag caaatgcatc
     6541 tgtcatcaat aaatttcacg agttagaaat gcctggaaaa atcggaccaa gctttgcgta
     6601 tacgccaaat tatccaattg acagtaatcc tgaaaatgtt ctggctgctg aaaatgcaga
     6661 agacctgatg gccaattatt ggatggatgt ctatatgtgg ggaaaatatc cgattgctgc
     6721 catgaggttc ttagaagaaa aaggatgggc accaacgatt gaagctggcg atgcagaatt
     6781 gctagaatct gctaaaccag attttcttgg gatcaattac tatcaaacgg cgacgaatgc
     6841 attcaatcca ttaaacggag ttggtgctgg aaaaatgaat acgacaggaa aaaaaggttc
     6901 atcagaagaa actggagttc ctggaatgta taaaaaagtt gagaatccat ttgtcgaacg
     6961 aacaaattgg gactgggaga ttgacccaga aggattaagg atcggactgc gtcgtatcac
     7021 aagccgttac cgcatgccgg tactgattac ggaaaatggc ttaggagagt atgacaagtt
     7081 gacagaagat aaacaaatcc atgacgacta tcgaatcaat tacttacaaa gccacgtcaa
     7141 agccatcaaa gaagcaattt cggatggagc agaagtccta ggctattgca cctggtctta
     7201 tacagatttg cttagctggc tgaacggtta tcaaaagcga tatggatttg tatatgttga
     7261 tcaagacgaa acacaagaag gatctttgga acgatataag aaagacagtt attactggta
     7321 ccaaaaactg attgaagaaa atggagagaa cctttagttc acatacttaa aacaagaagg
     7381 tacggctgga tatggccgtg ccttttcttg ataaaatcat ttagaaatta agaaaaactg
     7441 taaaataagc tgaaaaatga aagaaagcgt cagttctttc aaacaaaact ttagttccct
     7501 tacttttttt tgtataatga aacttgatga aatgagggat aataaatggc acaaaagaaa
     7561 cggcctacta aaaaaaggaa aaagacgaaa aaaacgaaaa agcaaaaaca gcagcaggaa
     7621 caaatgctga cgattggtgt tgggtttgcc ttgattttat ttgctgtctt cggcttttta
     7681 aaattaggtt ttttaggaat attgattgca aacggctttc ggatcattgc aggaaatact
     7741 taccaaatcc tctgtctttt attagccgta ttaggatttt ggattgtcat taagaataca
     7801 gagttttcaa ttggtaaaaa ccgacgctgg tttggcggca tactctttta tttcggtatc
     7861 ttattattgt tacatgccca tttatttggc aaactccata ctggggaacc aaatatcatg
     7921 gggacgacat gggatttgtt agccagtgat ataaaacaaa gtcaagtaga caataacgtt
     7981 ggtggtggga tgattggagc aatcctttat cattttactt acttcttgat tgcacagcct
     8041 ggaagttatt tagcagcagt gctattactg gcaggaggag cattcctgtt cagtaatctg
     8101 gaaggctatc agctgttaaa tggtatccaa tcaattggag aacgagtaca ggaattattg
     8161 gaaggtgatc ctgccaagca agctcgaaag caagcggcaa aggaagagag aatgaaacag
     8221 cgcgcagaag cgaaagaagc aaggcgctta gctgcaaaag aagctgcaga aaaagaagct
     8281 gcagaatatg agaagaaaaa agcggtgagc cagcaaaggg aaaatactgc tgatgaatgg
     8341 caggaaccag aaaatacaga gccggagcag ctaagttttg ttccaatcga cagttttcaa
     8401 gaaaatatcc atcctgcgaa tctagagaaa ccagtaccag atacaccaaa acagaccaat
     8461 acagcagaag gtttttcaga tgagttgcca gaggatgacg gtacctcatt agaatttgag
     8521 attgaagcag aacaggaaaa tcaagactat gaattaccga cagttgattt gcttgattct
     8581 ataccaactg tggatcaaag tgatgagtat aaaaaaattg aaaaaaatat cggcgtatta
     8641 gaacaaactt tccaaagttt cggtgtcgat gccaaggtcg tcaaagccag cttaggacca
     8701 tccgtcacaa aattcgaagt ccagccggct gtaggggtaa aagtaagtaa aattgtgaac
     8761 ttaacagatg atatcgcgct tgctttagcg gcgaaagatg tccggatgga agcaccgatt
     8821 ccaggtaaat cactgattgg tattgaagta ccaaatggaa agatcagtat ggtttccttt
     8881 agagaaatca tcgaagcaca accaaaccac ccagataaat tactagaagt acctttagga
     8941 cgtgatgttt ctggacgggt acaaacggct gatctttcga agatgcctca cttactcgtg
     9001 gctggttcga ctggtagtgg gaagtcagta gcaattaatg ggattatcac aagtatatta
     9061 atgcgtgcta agccccatga agtaaaactg atgatgatcg atccgaaaat ggttgaattg
     9121 aatatgtaca acggcatacc tcatctgttg acgccggtcg tgacgaaccc tagaaaagca
     9181 gcgcaagcct tacaaaaagt agtacaagaa atggaagaac gatatgaaaa attcgcggca
     9241 accggagtaa gaaatatttc tggttacaat gaatttgttc aacagaaaaa cttggaaaac
     9301 ggaacgaaac atccaacctt accattcatc gtagtgatcg tagatgaatt agctgatttg
     9361 atgatggtag ccagcaatga agttgaagat gcgattattc gtttagcgca aatggctcgg
     9421 gcagcgggga ttcatatgat cctagctact caacgtccaa gtgtggatgt tatcacagga
     9481 atcatcaaag caaatgtacc ttccagaatg gcatttgcgg tatcaagcgg tacagattct
     9541 cggacgatca ttgattctaa tggggcagaa aagctcttag ggcgaggaga tatgctattc
     9601 ttgccaatgg gtgagaataa accgatccgt gtgcaaggag cattcatttc tgaccacgaa
     9661 gtagaacggg tcgttcaatt tgttacagat cagcaagaag ctcattatga agagaaaatg
     9721 atgccgacag atgaagttga aacgacaggt gctccagaac agccacaaga tgagctgttt
     9781 gaagaagcaa aagcacttgt cgttgaaatg caaacggcaa gtatttccct tctccaaaga
     9841 agatttcgga tcggttacaa ccgagcagca cgtttagtag atgaactgga agctcatgga
     9901 gttgttggcc cgtcagaagg aagcaagcct cggaaagtat tcattgagca ggaagaaaca
     9961 tcagaaacgt tgccggacga taatgacatg taaaacttgt gtgatgaagg gttgaaaaac
    10021 gaataaccaa taaaaagaca aggcatgatg ataccaaaag aaaaaatggt ttttttccat
    10081 cttttctaat acggtatgat gtcttgtctt tttagttttt gtgagattaa agttctattc
    10141 gatttatttg tgaagtattg cattaaggaa atgaatcatg tatagtatgt agcgaaactt
    10201 tgagaaaaag agggaaaata ctttggaaaa acagtccatt tatggcttga cgaatgaaga
    10261 attaatcaat tggtttatag aaaacggcga gaaaaaattc cgagctgccc aagtttggga
    10321 atggttgtat caaaaacgtg taagtaattt cacagaaatg acgaatctat cgaaacaatt
    10381 gatcgaaaaa ttatcggagc attttatcat caatcctcta aaacagatgg tcgtccaaga
    10441 agcttcggat ggtacagtaa aatatttgtt tgaactccct gataaaaaca tgattgagac
    10501 tgtactgatg agacaagaat atggcttgtc agtttgtgtg acgacacaag taggatgtaa
    10561 tatcggctgt acgttttgtg caagcgggct gctcaaaaag aaccgtgatc tgactgctgg
    10621 agaaattgtt gcacagatca tgatggtcca acattatttt gatgagcgca atcttggcga
    10681 acgcgtttca catgtcgttg taatggggat cggtgagcca tttgataatt acgacaatgt
    10741 aatggacttt ttgcatatca tcaatgatgc caaaggattg gcaattggtg ctcggcacat
    10801 tacagtttcc actagtggat tagcacacaa aatcaaggaa ttcgctaata acggcttgca
    10861 agtcaatctg gctatttcct tgcacgcgcc gaataatgag gtgcgaacat cgatcatgcg
    10921 gatcaaccga agtttcccaa ttgagaaatt gatggaagct gtggatgagt atttagagaa
    10981 aacaaaccgt cgaatcacgt ttgaatatat catgttgaat caagtaaatg atagaccaga
    11041 gcatgcgcaa caattagcag atctattaaa agataagaaa aaattagcat atgtcaactt
    11101 gattccatat aatccagtca gtgaacatga tcaatattct cgcagtccaa aagctgatgt
    11161 attgagattt tatgatgtat tgaagaaaaa cggcgtaaac tgtgtgatac gtaaagaaca
    11221 tggtacagat attgatgcag cttgcggaca attgcgaagc aagcaaatga aaaaaacagt
    11281 tgccaaataa tcgtatccta ctcgtttttt caattaccat aaaaggagca gttttcttaa
    11341 ttaaggaact gttcaactta aataagagaa aagattctat agcgaaagct ataaagcttt
    11401 ttttgacaag aaaaaccctc atcaagcaaa gaaatgtcct caatttgatg acagcctgct
    11461 tgatgggggt tttagcaatt atttaactga ttttcttata tccttgctgg ctttcttgtg
    11521 tagcatgata agtttctacg tcaagaatgg cctgtttaaa tttttctcgg gtgatttcat
    11581 aaggcacatg tttaatatca tttgttgcca tggctttatc taagattgct tctaaatcgg
    11641 tcgtgacatt gagattcatg tcggttaacg atactggaag ttggttcttt ttcataaaca
    11701 catagatttt atcaaacaaa tcgaactgct gatccatcat caataaggtc agtacaccat
    11761 aactgacaga tgctccgtga aggtatccct ccattttttc tagaacagtc gtaccgtaat
    11821 agaatgagtg tgccatagca gaattatagt tattgttaac taaaacagaa accaatccgg
    11881 tagtaacaat gatatccgaa acgacttgtt caattgcata tgagggacga tcgtcggcac
    11941 tatctttcaa tgcttgcgta ccataagtca agagacgatc aatgcacatt tttccgattt
    12001 ggattcctaa tctatccgtg tagcctagat catcgccacg agcagaaaaa gtagattcga
    12061 cttctttgga taaagcatcg ccaatacctg cccataagta tttatcaggt gcttcggcaa
    12121 taattgctgt atcaatgaag gtgtgtacag gtggttttga aggaaagaag agtccgtcca
    12181 ttacgccttc ttcgttatag atgacacata ccgcagtgac gggcgaacag ttcgaaccaa
    12241 tagtgggaaa ggtgaagact ggtttagcta gtttttcacc aatgactttt acagtatcgc
    12301 aagcacggcc accaccgact gcaaacagga cgtctgcttt ttggacactt tcatgctgga
    12361 tcaactgttc aatatttcca taagttgaat tacccccgta ccagagactg gcgacaacct
    12421 ctatttcctg tgtcaaagct acttttattt tgttttctgc ctttgaaagt gcagtcttcc
    12481 ctccaatcag cactactttt gtcccatatt ctttgacgat tccatttgct ttttcatacg
    12541 catctgttcc aatggtataa tttggtaaga aaacactttg actcatattc atttccttct
    12601 ttcttcgttg aaacgggtta aaattttaaa gagtgatcga tctgactgcg gctgtagatg
    12661 gagtagaggt gaacctaaga caggataagt gatgaataag gtggcatgaa caccttgaaa
    12721 tcatcaataa gtatcctaaa aacgaacaag tacaaaaaca ttgaaaacga caataatgaa
    12781 tttttgttcc tcttagtatt tctaccgatt ccctttgctt ttttgtaatt atctaaaaat
    12841 tctaaaaatt aagattatta tatgaaaaca ggttttattt gtcaattcat aattggtttt
    12901 ttttaaagca aaagataatc tatatcatga tgaacaaaca gagttttttc ctccaattga
    12961 tggctttcct gatctttcct attttgaata aagtgtttct gtataatttt tttattttat
    13021 aagtaagata aataatgaaa gaatagagat actgattggt agctaaagat ttttgagcga
    13081 gaacatttcg ttttttcaaa aggactgtta gaataagcaa gaacaagggg gaagacgtat
    13141 gataatcagt gaatttgacc gtaataatcc agtattgaaa gatcagcttt ctgatttact
    13201 gagactgact tggccggaag aatatggaga cagctcggca gaagaagtag aagaaatgat
    13261 gaatccagaa cgaatcgcgg tagcagcggt agaccaagat gagttagtag gatttattgg
    13321 tgcaatccct caatacggta tcacaggttg ggaattgcat ccattagttg tagaaagctc
    13381 ccgacgaaag aaccaaatag gtactcgatt agtcaattac ttagaaaaag aagtagcttc
    13441 cagaggagga atcacgattt atttaggtac ggatgattta gaccatggaa caacgttaag
    13501 tcaaacggac ctgtatgagc atacatttga taaagtggct tccatccaga accttcgtga
    13561 acatccgtat gaattctatg aaaaattagg ttataaaatc gtaggtgtct taccaaatgc
    13621 aaatggctgg gacaaaccgg atatttggat ggcaaaaacg attattcctc gaccaaattc
    13681 tcaataataa aaagaggttg tgacagaagt aatcagcttc aagaaataag aaggaattta
    13741 cgaaaattac ttcttgaatt tttgtgaaat tccaacttat ttccgaagaa gctacttttg
    13801 gaacaccgtg taccagaggt tgtgaaaaag tcgcttagtt tcgagtagta agaaaaattt
    13861 tgtgaaaatg gctttctaca tttttgcata aatttggctt aataccgaga aactggcttt
    13921 tgaacaccgt ttattcagat aagggactgt gacaagactt ttgtcacagt ttctttttgt
    13981 ttattctttt ttatctttgt atggcaataa tagacaatat aacgaaaatt atttaatggc
    14041 ttttggaaga ccatggaaaa ccttgtacaa agctattttt tctgaaaaat tccttgaaac
    14101 ctcacgaaaa aatcattatt ttatgaaaaa gacaaaaaaa agtgttgatt ttttaataat
    14161 tattcattaa actcttaaac aacttcagaa aatattaatt attctgaaaa ttccaataaa
    14221 ggggtacgta aaatgaaaaa gaaatttaca tttggtttgg ttgcggtatg cggccttgta
    14281 cttgctggat gttatggcgg cagttcagcc acaagcagta gttcagcaaa aagcggttca
    14341 gcagatggag gaggcgtatt taacttagtc gtgcctcaag aaatgcctac agctgactta
    14401 tcagttgcaa cagatacaat cagttttaca gcattgaata acgtttatga aggaatttac
    14461 cgtcttgatg aagacagcaa accacagccg gcaggagcta gcgagttggc agaagtcagt
    14521 gaagacggat taacttacaa aatcaaatta cgagaagacg caaaatggtc aaatggtgat
    14581 cctgttacag cagcggatta cgtttatggc tggcaacgaa cagtagaccc agctacagct
    14641 tctgaatatg cttacctgta tgcgccagtt gaaaatgcag aagacattac agctggaaag
    14701 aaagataaat cagaactagg gatcaaagca gttggtgact atgaattaga aatcaaattg
    14761 acgaaacaaa caccatattt ccaatatctg cttgctttcc catcattttt cccacaaaaa
    14821 caatccgtag tggaagaaaa tggggatgcc tatgcatctg caagtgataa agctgtttat
    14881 aatggaccat tcacattaga agattttgat ggaccaggaa cagatacaga atggacttat
    14941 aagaaaaatg atcattactg ggacaaagat acagtaaaac tttctgaaat caaagtcagt
    15001 gtagtaaaag aatcttctac tgcattgaac ttattcaaag atggccaagc agatgacgtg
    15061 atcttgtctg gtgagttagc acagcaaaac gcaaacgatc cagcttatac ttctgttaaa
    15121 gaagcgcgga caagctatat cgaattcaat caacgtgaaa agaattcacc attcaacaat
    15181 gtgaacctaa gaaaagctat ttcttattct atcgatcgtg aagctttagt aaaacaagta
    15241 ctaggtgacg gatcagttgt ctctacagga ttgatcccat cagatatggt aaaaagtcca
    15301 acaacgaacg aagactttgc aaaagaagct ggaaaattag tttcttatga caaagataaa
    15361 gcaaaagaat attgggaaaa agcgaaaaaa gaattgggcg ttgattcttt agaatttgat
    15421 ttgatggctt ctgatgatga ttcatcgaaa aaagtaatcg aatatgttca aaactcaatc
    15481 caagaaaact tggatggcgt aacagtcaaa ccaacacctg ttccattctc tgtacgtctt
    15541 gaccgctcaa cttcgggtga ttttgacgca gtactaggcg gatgggctgc tgactatgca
    15601 gacccaagca gctttactga tttgtttgtc acaggtaatt catacaaccg tggtcgctgg
    15661 agcaatgcag aatatgacga agcagttaaa gcagcttcat caacagacgc tggcgatgaa
    15721 caagcccgtt gggatgattt ccaaaaagca atcaagatca tctctgatga tatgggtgtc
    15781 gttccggtgt accaaaaagc agaaggtcac ctagtgaatg aaaaaatcaa aggtatcgtt
    15841 caccatgcgg caggcgcttc atgggattac aaatggacat acgttgaaga ataatcgtta
    15901 ataccgtgag catgagaaaa aacgaggctg cggcaaatgt cgcaacctcg ttctcatatt
    15961 cacttatcct aacaaagaaa aaagcatgaa aatagcagaa agagcaatta gtattccggc
    16021 aatgatcagc caaaagctgt acataccgta gctggcagac gagagcgagg aaaattctgg
    16081 ttttttcttt cggttccttg ttcgtgcaag atggaccgat cgctgaaaat gatcgaagaa
    16141 acccgaagaa aagacccata aaaaaccgcc gataatcaga aacggcaatg accataagaa
    16201 tagatcatta gataattgaa tgagtgtaaa ggaatggttg atgagatttt ttattaaaat
    16261 aaccagaatc agtagaaaag ctactgcgta ggggatagat ttttttttca tttgattgct
    16321 cctttatttt tggttcatta tctaataatg tagcgaaaca tctgtcagaa gtcaaaagct
    16381 agggggaaac attagtgaat agttatatca aatatgtatt aaaacgtgtc tttttcatga
    16441 tcatcacgct ttggttaatt gcaacaatca cctttttctt gatgcaattg ctgccgggca
    16501 cgccatatac gaatcaggaa cgtttgagtc ctgaaacaat cgagatgcta aataaacaag
    16561 tcggtttgga taaaccagtt atcgtacaat atgggattta tctttctaat ttgcttcaag
    16621 gagattttgg aatttccttc caatttaaaa accagccagt cgcacatttg ttagctggaa
    16681 gaatcggacc atccttgcag ttagggttac aggcaattat ctttggaaca gtcctaggga
    16741 cgattttagg aacgatctct gccatgaagc aaaatacatg ggcagataca tcttctacac
    16801 tcgtagcgat attaggtcgt tccattccaa actttgtatt tgctgtttta ttacaatata
    16861 tctttgcaat caaattacaa gttttgccaa tcgcaaaatg ggatggcttt gtgtacacaa
    16921 tcttaccaac aattgcgttg gccatgtcgc cgcttgctga ctctgctcgg tttatccgta
    16981 cggaaatggt ggaagtactg catagtgatt atgtggaatt agcaaaagca aaaggattga
    17041 gccgttggga aatcgccttt aaacatggtc ttcgaaatag tttgattcca ttgatgactt
    17101 tattaggacc gttagctgta gcattgatga ccggatcact ggtggtagaa aatatctttg
    17161 ctattcctgg tatcggggaa caattcgtta agtcaatcac aacaaatgat tatcctacga
    17221 tcatggctgt aacgatcctt tactcattta tgctgatttt tgttattttg atcgtggatc
    17281 tattgtacgg tctagttgat ccaagaattc gtgtttcaga agggagccga ggataaatgg
    17341 aaatgatacc aaagcgttat gaaacagttg cagatatccc tgcggatgaa tttcttccat
    17401 tacaaaaaaa taccgaagaa gaacgtgaac agattactgc accttctttg aattttatcc
    17461 aagattcatg gcgccgttta aagaaaaaca aagctgccgt tatttcaatg gttttactat
    17521 tggtcatcat tttgatctct gtgatcacaa tctttgtttc tcctcatgat ccaactgccc
    17581 aaaacgtggc ttatatcaat ttgcctcctc gtattccggg aatcaatatc gatggattga
    17641 atggaaaagc aatggttggt ggtgaacttg ttgataaata tgctcaagca aacgtccctt
    17701 caaacgtaaa tttcttttta ggaacagacg gtttgggacg tgatgtatta agccgcttat
    17761 ttatgggaat gagaatctcg ttgctgatcg catttattgc tgcattacta gatgtaacga
    17821 ttggtgtagc ttacgggttg gtttctggtt tgttaggcgg acgtgtcgat aacgtgatgc
    17881 aacgtttttt ggaaatctta tcaggaattc caaacctcgt tgtcatgatt ttgatgttgg
    17941 tcgtttttga accaggaatt ttttcaatcg tggctgctat ggccatcaca aactggatac
    18001 caatggcacg tatcgttcgt gcacaaacaa tgaaattaaa agatcaagaa tatgttttag
    18061 ctggattgac attaggtgaa tcaagaatga aaattgcatt caaacatatt ttgccgaata
    18121 tctctagtgt cattattatt caaatgatgt ttagtattcc atctgctatt ttcttcgaag
    18181 cctttttaag ctttatcggt ttaggtctta cgcctccgtc agcttcatta ggaacgatgt
    18241 tgagcgaagg gtacaagaca ttcctgtatc tgccttatct attatggatt ccggcagtaa
    18301 ctttatcggt tatcatgatt ggctttaact tacttgctga tggattgcgt gatgcctttg
    18361 atcctaaaat gaaagagtga agacgatgac gaaaatttta gaagtgaaag acttagaaat
    18421 ctcttttgat acgtatgctg gaaaggtacg agcgattcgc ggtgtcaatt ttcatttgaa
    18481 taaaggagaa acattggcga tcgttgggga atctggaagt ggaaaatcag tgacgaccag
    18541 aagcatcatg cgcttgctgt caagcaacgc caatatagat gctggtcaaa tcctctttaa
    18601 aggtaacgat atcgttggaa aatcagaaag agagatgcaa aaaatccgtg gtaaagaaat
    18661 agcgatgatt ttccaagatc caatgacctc tttagatccg acgatgccca ttgggaagca
    18721 agtcgctgag tcccttagaa agcataataa agtttcaaaa aaagagggac aaaaagctgc
    18781 attagaattg cttaaattag ttggaatacc cgaagctgag aaacgtatca acagttatcc
    18841 gcatcagttc tcaggaggtc agcgtcaacg gatcgtgatc gcgattgcac tggtatgtta
    18901 tccggaaatc ctgattgctg atgaacctac aacagcgctt gatgtgacta ttcaagcaca
    18961 gattttggag ctgctgaaag aaattcagac aaaaatcgat acatcaatta tctttatcac
    19021 tcatgacctt ggtgtagtag cgaatgtcgc tgatcgtgtc gctgtcatgt atggaggaag
    19081 aatcgttgaa gttggaacat cggaagaaat tttctacaac ccgcaacatc cctatacttg
    19141 gggactgctt ggttcaatgc cgacattaga tagtgcgaac gatcgattgt atgctattcc
    19201 tggttctcca ccggatttat tgaacccgcc aaaaggagac gcattctatc caagaaatga
    19261 attcgcgatg aaaatcgatg cagaacaaga accaccattt tttgaactat caaaaacaca
    19321 caaggcagca acttggctac ttgctccgca ggcacccaaa gtgacaccgc cagaagaaat
    19381 ccaacgtcgc tgggctatct ttaaagagaa acaaaaacat acttacggtc aaggaggaat
    19441 tgctgatgcc aaataaacta cttgaagtta aaggattgaa acagtatttc aatgtaggaa
    19501 aaaagaatga agtacatgct gtaaatgaca tcagctttca catttatgaa ggagaaacat
    19561 tcggtttagt tggagaatct ggaagcggaa aatccactac tggccgtacg atcattcgct
    19621 tgaatgaacc tactgatggt gagatattat tcgacggtca agatataacg aagatcaaag
    19681 ataaaaaagg tttgacgaaa ttccggcatg atgttcagat gatctttcaa gatccatacg
    19741 cttcgttgaa tcctcgtatg aaagtcagag atatcattgc cgaaggaatt gatgttaatg
    19801 gactagctaa atcatctgaa gagcgtgcaa aaaaagtcga tgatctattg agaactgttg
    19861 gattgaatcc aagccacggt actcgttatc ctcatgaatt ttcaggtggg caacgccagc
    19921 gtattggtat tgcacgagca ttggcagtaa aaccgcgttt tatcatttgt gatgaaccaa
    19981 tttctgcact agatgtgtcg atccaagccc aagtggtcaa tttgttgcaa gatctccaaa
    20041 aagaacatca attgacctat ctatttattg cacatgattt gtcgatggtt aaacatatta
    20101 gtgaccgaat cggtgtaatg cataacggct tgttattaga gatgggaaca agtgatgaaa
    20161 tctataatca cggtgttcat ccttatacag aaagtttgtt atctgctatt cctctaccag
    20221 atccagacca tgagagaaaa cgcagaagaa tcaaatatca acctgaacca gatgacggac
    20281 aagtacgtca acttcgtgaa atcgcaccag aacattttgt ctatgcaact gagcaggaag
    20341 tcccatatta cgcaaaaaaa ctaaaacgac aaaaggaatc tcttctggtt gctaactagg
    20401 agggacatag atgctaaaag cttttaaatt ccgtatgtat ccgacagaag aacaaaaaca
    20461 gcaattgatt cgtacttttg gatgtgctcg atttacctat aaccatttgc tgaaaaagcg
    20521 gcagaaaagt tggcaacaaa caggagtagc caatttttcg ctcacacccg ctacattaaa
    20581 aaaagaatac ccattcttaa aagaagtaga cagtcttgct ttagcaaatg cacagcttaa
    20641 tttggatcgg gcttttcgca attatttcaa gggacgtgcc agcttcccaa agcttaaaac
    20701 gaaaaaaagt atgtggcaat cttatacgac caacaaccaa acccgtacga tctatctgga
    20761 aaatggctac ttaaagttac caaaacaaaa agaactaatc aagataaatc agcatcggcc
    20821 agttgaagga tcgattcgct ccgctacgat ttctgctcga tataatgaag aattttatgt
    20881 cgcactactt tgtgatgttt cgccagtaaa aaaagaatct ttagctaaat ggatcgggat
    20941 cgcctatcat ccgaagacat tgatccaaac atctcgacct ctagaagtaa ctttaccaaa
    21001 atttcaccag acagaagaaa agctgcaaca tgcacagcgg aaattgaatg tgaaagtccg
    21061 ctccgctcac catagaaaga tcaggttaga tcaggcaagc aattatcaga aacaaaagag
    21121 aaaagtaatg gatctttatc tgaaacaaaa gaaccagcga gaagattatc ttgaacaatt
    21181 atctgggaaa ttggtcaaac aatatgacta cttatttgta gaatcttttc caaaagaaga
    21241 ggcacatgct gatttttcta ttcacgactg gcataaatta ataacaaaat tgcgttataa
    21301 gtcccaatgg tataataaaa aatttctttt gatcaatacc gacggagcag aagaaagcaa
    21361 ttccgtaaga aagagccaag tagtagaaaa aatgggcaga cattcggtta tcaagggata
    21421 aaaaatgaat gaaactgata ggagcgttag ggttagcctt gttaataaaa agtcttaatg
    21481 acttttgttt ctaggaattt ttcttttgaa gaaaagctca ctaattatga caaaccaata
    21541 aaacaatagc agaaaaagtt cgtacgagct ttttctgcta ttgttaattt tttattcaga
    21601 aagtttacga atacatcaaa attctgaaac aaaaaacgta actcacaaag agttgttttt
    21661 gcgttttttc ttgaatgatt aggtctgaat cttttgctca caaccttatt catggtgccc
    21721 cggcttgagc tgatagattt ttgaaggacg gccgattcct acaggacgtt caccgatctc
    21781 atcgaaatat tggagcatag ctttcttaaa attggaatga tcgatcgttt tataatcgat
    21841 ccctaagaat ttagcaaata cttttcgagc ttccgtaatc gtgaaatctt tacctaaaac
    21901 ttgtagaact tgcggctcgt gttccatttt attgactaca cggttgaatg ctttcaagat
    21961 gatttgacta tggtcaaaag ccaaagtatc tttgccagaa gactctccag tgactaaatc
    22021 aagcgagata gccacttctc cgctagataa atggatcttg ttactatgac gttctagtgt
    22081 gaaccagcgt acttccttgg catcatctcc ggcgattagc ggttcttctc caataaaggc
    22141 taagtagctg actgtgacga cccagcctcg cggatctcga ttaggcgtac tgaatgtatg
    22201 cagttgctca atattttctt ttgaaataac cacaccagtt tcttcctttg tttctcttaa
    22261 gacgctttct ccagttgact catccttttg gacaaagcca ccaggcaatg cccatgaatt
    22321 tcgataagga tgcccttttc tttggatcaa caaaactttc agctgatctt cttctttgtt
    22381 atagcacatc agtacgatat cgacagtcaa agagggcttg tcatattctg gacgttcctg
    22441 tttttgatac cagttaagaa attcttcttc acttgcttta tgttcataat ataatttttc
    22501 ttcttcttta gaagcaaaac ggttcatctg accacctcca ttgacagtaa aagtactttt
    22561 taccttattg tagcagactc tgaaaaaaaa ccaaggattt acttgaaaaa tctaggttat
    22621 catttaaata tgaaaaataa atggaagtat gagattgaat aaaaagaaaa gcagataaaa
    22681 cggcaatact ttataccgct cttcacgaat ggcaaagaag ccacaaataa cgatatatac
    22741 gtcaatgata aaacgcaaaa tccaagctgc tgtcataaaa ctgttcaaat gcccatggag
    22801 gttaaaataa ccatacatac gaatataaaa atagctgaat attaaatgtg ctaatgaaag
    22861 aaaagcaata ctaagtgtaa aaaaaagatg tttcttctgc atgattgttc cagtccctta
    22921 ctaagataga tagtctttat tataccacgt gtgaggggag aaataaaaag ccgtttttta
    22981 gtagatatga aacaaaacaa aaacccgcat ttattcaacc tattcatcct tttaaaggat
    23041 agattgaacg tagatagaag tcgtttatcc agtctttttc attttgaaaa aaagctaaca
    23101 gcatgctttt tttatggtat actagcaagg aaacgaattc gcatcatgcg ataaaggagg
    23161 aagagccccg tggcagaaaa ggaaactttt tatatcacta cgccaatcta ttatccaagc
    23221 ggcaaacttc atatcggcaa ctcgtatact acgattgcat gtgacgcaat tgctcgttat
    23281 aaacggttga tgggctttga tgtgttttat ctcactggag tggatgaaca tggtcaaaaa
    23341 atcgaaacaa aagcagaaga actaggtgta aaaccgcaag aatatgtaga taaaatggcg
    23401 gctgatgtca agaaattatg gaaaacgctt gatatcagtt atgataaatt cattcgcacg
    23461 acagatgatt accataaagc agctgttcaa aaaatatttg atcgtttatt agaacaggga
    23521 gatatttact taggtgaata tgaaggctgg tactctgttt ctgacgaaga attctttaca
    23581 gaaacacaac ttgcagaagt gtacaaagat gacgaaggta acgtgatcgg tggtaaagcg
    23641 ccaagtggac acgaagtaga attggtaaaa gaagaatcat acttcttccg catgagcaaa
    23701 tacgcagacc gtctattgga atactatgaa gaacatcccg aattcatcca gccagaatct
    23761 cgtaaaaacg aaatgatcaa caactttatc aaaccaggat tagaggatct tgcagtttca
    23821 cgaacaactt ttacttgggg agtaccagtc aagagtgatc cgaaacatgt ggtgtatgta
    23881 tggatcgatg cattatccaa ttatattact gcactaggat atggctccga agatgaaagt
    23941 ctattcgaaa aatactggcc tgcagatgtt cagatggtcg gtaaagaaat cgttcgcttt
    24001 catacgatct attggccaat catgctgatg gcccttgatt tgcctctacc taaaaaagta
    24061 tttgggcatg gctggctatt gatgaaagac ggaaaaatgt ctaaatcaaa aggaaatgtc
    24121 gtttatcctg aaatgcttgt ggaacgttat ggtttagatg cactccgtta ttatttgctt
    24181 cgtgctgttc cgtttgggtc agatggtgtc tttacgccag aagattttgt ttcccgtgtc
    24241 aattatgatc tggcaaatga tctagggaac ttattgaatc gaacgattgc tatgatcaac
    24301 aaatattgtg atggactcgt tcctgactat gcctcacttg tcactccatt tgatagtgaa
    24361 ctgtctacga cggctgctaa cgttgtcgga cgttaccatg atgcaatgga aaaaatggaa
    24421 ttcaatacag cattggctga aatctgggtg ctgatttctc gtgcaaacaa atacattgat
    24481 gaaacagaac catgggtatt agcaaaagac gaagaaaaga aagctgaatt gaatagtgtc
    24541 atgatccatt tagctgaatc tttgcgtatc gcagcgatat tacttcagcc aatcatgaca
    24601 gaaacgccag agaagatctt caatcaacta ggattggact ctgaaacaat gaatcttgaa
    24661 ggattacact ttggcgaatt tccttcagga acaaaagtag ttgcaaaagg tacaccaatc
    24721 ttcccacgtt tagatatgga agaagaagtt gcttttattc aagaaaaaat gtctgaaggt
    24781 actcaaacaa acgaagatac agtcaaatgg gatccagaag aaacagaact tgtttcaacg
    24841 aaagaaaaac aaatcaaatt tgatgtattt gaaaaagtag aactaaaagt agcagaagtc
    24901 atcaactgtc aaaaagtaga aggggcagat aagttgctgc aattccgact agatgctgga
    24961 gacagccaag accgtcaaat cttgtcagga atcgctgaat tttatcctga tccaagcgaa
    25021 ttgatcggga aaaaagtcgt cattgtagca aatctgaaac cacgaaagat gcgtggacaa
    25081 atcagtcaag gaatgatcct ttctgcagaa gcaccagatg gctctttaca agtaatcgaa
    25141 gcaccaaaat ccatgccaaa tggctcagaa atcgcataga aattcaaagg gggtatgaca
    25201 ctagttgtgt cataccccct ttttccgaaa tttgagaagc aattttcgta aattccttct
    25261 tattacttga agccaatcat ataatacaga aacagagaac tatccaaaat aaatcctaga
    25321 aaaagaagta cgagcttcgc tgatttttgt taatttagaa taatactaat aggaatcgta
    25381 aaaacagaaa aaaagcataa tgataaaggg tcgggcagat tttaattgat tcatctggta
    25441 ctctttttta ttgtccttac attcataaag tttgaatgca atgactttat tacatctata
    25501 tttatatcta acagtagcgt tgatgtgtta attataagtt ctatcacttt atgttcaatt
    25561 agtttgtgct cttatatgtg tgattgaaaa tatagataaa aaaacaatca tcggctttga
    25621 cttcgatgat tgttttcttt attttgtaaa aatcctatca cttgatttta tggtaacctt
    25681 ttatttttgt cttcaaacgg cttggtaaga gagcagtaag cagtgcaaat gggatcgaaa
    25741 agacatacca gaaaatcgta cgtccgatac cataatgttg atagagcttt gtcaacatca
    25801 cgtgtaacaa gatcaaccct aaaagcaagg agccagctat aaaccaatga ttatcccaat
    25861 gcatgacttc actaagtgcc atcactccaa aaggatagaa ataaaaatag atcccgacta
    25921 aggtcatccg ccagttcttt gtaaatgttc cgatattcaa taaaaagaaa atcgctgaaa
    25981 gcaaaatacc gataggcgga aaaagcccaa ccaaaaagga atacaacatc ccccacaaga
    26041 tcatcacagg acctttgatc aatgcaacga ttgcgatgaa caaagtcagc aatattagtt
    26101 tttgtgataa cgttgcaaca gtcattaatc caaataaaat taatgtgaac caatgccaat
    26161 gggactgctg ttgttcttta cgggaaaaat cgacccggtt ggataaataa tctgctaatt
    26221 tcggataaga acgcttaaaa tccataaact gttcctcctt cgtggaaaag gtaggtttag
    26281 tatacgaaaa ataaaaaaga aatacatccg tcttaagagg gagtttttca ctgaaagtgg
    26341 agaaaaaaga atggtaaatt aaaatattct cagttatact gaaaattata ccaaatttca
    26401 aagaagaaag aggatgggaa tgaaaggcaa actaaaacga gaaatcaatc tgtttggcgc
    26461 attagctacc gttatgggaa ccgtgatcgg tgcaggcgtc ttcttcaaaa ctgctgctgt
    26521 tactgctagt actcagtcta caagtttgac attgctagct tggcttttgg gtggtttctt
    26581 aacgatctgt gctggattga cagtggctga attagctaca gcaatcccgg aaactggtgg
    26641 agctgtaaaa tatattgaag ctgcgtatgg aaaattgccc agctttttat taggatgggc
    26701 gcaaagcttg atttactttc ctgctaatat tgctgcatta tcgattatct ttgcgacaca
    26761 attgacgaac ttattacaat tatctaccga ttatcttttg ttgattgcag tgatcaccgc
    26821 tgtttctgtg acagggttga atttgttagg gacaaaagtc ggtgcttctg tacaatcagt
    26881 gacgttgatt gttaaattaa taccaattgc tgtgattgtc atatggggat tattgacccc
    26941 aggacaagga actgtccagt tatttccaat cgaagctgga aaagatgtta cttttgtcga
    27001 aggattgagc agtgcgttgt tagcaacgtt gtttgcttat gacggctggt taggtgtcgg
    27061 tgcaatggca ggggaaatga aacggccgga aaaagatttg cctaaagcaa ttattcttgg
    27121 tttgagtttt gtaacagtcg tttatctatt gatcaatttt gtttttttga aaactttgcc
    27181 gattgatcat cttgccggta atttgaatgc agcttctgaa gcatcagacg tcatttttgg
    27241 aggaatcggt ggaaagctgg tcactattgg gatcttgatc tcggtatacg gtgcattgaa
    27301 cggttataca ctaactggga tccgcgtgcc ttatgcgatg gctttagaag acgagctacc
    27361 tttcagtaaa cagctgacaa agctttcaaa gaagtttacg gttccttatg ttcctgcggt
    27421 tttccagtta gcagtagctt gtattatgat gagccttggt tctttcgact ttttaacaga
    27481 catgttgatt tttgtgatgt ggctattcag tttactgatt tttatcggtg tctttatttt
    27541 aagaaaaaaa gcgccggaat tgccacgtcc atataaagta ccactttatc caatcgttcc
    27601 gattattgcg attctcggtg ctattttcat tttaggtatg acgatgatga cgcaaacgaa
    27661 attagcattg atcggtatag gtgttacctt gataggtatc ccagtctatt atcaaaaaaa
    27721 gaaaaaacgc tcagaagagt aaaatatttc aacaacagac agaaaaagta cgaactaggt
    27781 cctctagatc ttcgtgcttt ttctatttca ataaaacaag ttcttctatt ttgatttttg
    27841 ctacttctgc aagtagtgct tatgtgctaa agtaagaatg aaaaggagag ataaacatga
    27901 tatttgattc tcatacccat ttaaatgctg aacagttcaa tgaagatatt ccagaaacga
    27961 ttcagcgagc acaagaaatg ggggtaacga aaatggcggt cgtgggcttc gatacgccaa
    28021 cgattgaaaa gtctttgcaa ttaagccatg actatccaaa tatatacagt attatcggct
    28081 ggcatccgac agaggctggc agttatacga aagaaatcga aaataaatta caagaacaat
    28141 tgactttacc aaaggttgta gcattagggg aaattggctt ggattatcat tggatggaag
    28201 acccgaaaga agtccaagat aaagtatttc gcagacagat tgctatcgca aaagaaatga
    28261 acctaccaat cagtattcat actagagaag cacttgaaga cacgtatcaa atcctaaaag
    28321 aagaagatgt tcgagatatc ggtggaatca tgcatagttt cagtggtgat tatgaatggg
    28381 caaaacgatt tcttgactta gggatgcata tttcatttag cggtgtcgtc actttcaaaa
    28441 aagcattaga cgtccaagag gctgcagcaa atgtaccaat cgaacgatta cttgtggaaa
    28501 cagatgcccc ttatctggca ccagttcctt ttagaggaaa gcgaaatgaa ccaggctata
    28561 ctcgttatac agtagaaaag attgctgaac ttcggaatct tccgacagaa gaagtggctg
    28621 cccaaacatg gacaaatgcg caccgattat ttggtctaag cgaacatgga taagatgaag
    28681 atccaagaga ttatagtcgt tgaaggaaaa gacgatacaa gaaggctgcg ggaagtagtt
    28741 gatgcagata cgatagaaac aatagggtct gcaatcaatg aagagatcct gatgcaaatt
    28801 gaacatgccc aagagactcg tggagtgatc gtttttaccg atcctgattt ttcaggagaa
    28861 aaaataagaa aaatcattat ggaagctgtt cctcaggcaa aacatgcttt tttgccgcgc
    28921 acacaagcta aaccaaagaa aaagggaagc cttggtgtcg agcatgcaag tgatgctgct
    28981 attttagaag cattgaaaaa ggtcgtgacg ccagacattt cccaaacggc tgtttctgaa
    29041 atcactagac aagacctttt agactatggt ttaatagctg gggcacaagc aaaagaaaaa
    29101 cgagaaaaac taggtgacga gttgcgcatc ggttacacga acggcaaaca attggaaaaa
    29161 cgtttgaaga tgtttcgtat tacgccgaaa gaactcgccc aagcaatgaa aatagtggag
    29221 gaacaacatg agtgaatatc gtgaaattgc gacaccctct agaacaaaag agattttatt
    29281 gaaacatggt ttttcattta aaaaaagttt aggacaaaat tttctgactg aaccaaatat
    29341 tttaaggaaa atcgtagaaa ctgcgcaaat agatgaacat acgaacgtga tcgaagttgg
    29401 accaggaatc ggtgcattga cggaacaatt agccaagcat gcaaaacaag tcgtagcatt
    29461 tgagatcgat gatcgtttga tccctgtatt ggctgatacg atgcaaccct atgacaatgt
    29521 gactattgta catcaagata ttttaaagac cgatttgtca acagctgtta gagaaacatt
    29581 tcatgaggag cttccgttaa aagtcgtagc gaatttacct tactatatta cgacaccgat
    29641 catgatgcat tttcttgaat cagatttggt tgtggatgaa ctagtcgtca tgatgcaaaa
    29701 agaagtagct gatcgtatct ctgctgaacc aggaacgaaa gcttatggtt cattatcgat
    29761 tgctgttcaa tattatatgg aagccagttt agcattcatc gtaccaaaaa cagtatttgt
    29821 ccctcagcca aatgtagatt cagcgatttt gaaactgaca agaagagata taccggcagt
    29881 cgaagtgaca gacgaaaaag catttttccg attgacaaaa gcggcatttc agcaaagaag
    29941 aaaaacgctt tggaataact tgcagcattc gtatggcaaa gatgaccaga caaaagcatg
    30001 gttagcaaaa agcttggaaa cagctggaat cgatccaaaa cgtcgtgggg agactttgtc
    30061 attacaagaa ttcgccgcat taagcaatgc gatgtcagaa aatatgcagt aaaaaaagag
    30121 gttgtgacag aagttgtgaa aaagctgtcc tgcatcaagt aataagaaca gccaaacaaa
    30181 aatagttcct catgtttttc gtttgtttgg gcttgttacc gcagatgcaa gcttttgaac
    30241 accgtttaag aaagaggttg tgggcctgcc gtttaactct aagtcacaag gaacaatttc
    30301 aaaaaacagc ttctcatgtt tttgagttag ttggtcttat tacttgatgc aagcttttga
    30361 ccactattat gccgaacaag gagatgtgac acaaattttg tcacatctcc tatttttgtt
    30421 tcttgatcaa aaaattctta tacttgatac tcacactcat tttgtatgga tttagaaaag
    30481 aatatgtgtc ttgacagtta ttagtaagtg agttatacta agtcgacatt tatgtgtcaa
    30541 gacatattag gagagagaaa aaatgagaaa aaattcagtt cgaaaactaa cgatttctgc
    30601 tttattgatt gggatggggg tcattatccc aatggtcatg cctaaaatca tgattccacc
    30661 agcttccttt actttggcgt ctcatgtgcc attatttatt gcgatgtttt ttacaccagg
    30721 agttgcagtg gctgttgcgc taggtacgac atttggtttc tttttaacaa cgccggttat
    30781 catcgcattg cgagcattgt cacatcttgt gtttgcgttg attggggctt ggtacttgca
    30841 aaagcatccg agtatcgttt taaaaaatgg acagtttact tttgctaatt ggcgtttcca
    30901 agggttcaat tttgtgatcg gtgtgattca ttctttagca gaaatgctag tagtcagcat
    30961 cttttacttt attggtaata tgcctgaaac atattattct caaggatatt tttatacagt
    31021 attcatcttg atggggattg gtgggcttat ccacagttta gttgattata atattgctta
    31081 ttttatagca ggaacactca gcaaatattt tgatatcccc gtatttacgg cagcaaaaaa
    31141 agaaaatcgg aaagagtcga ttcaagggta cgcgaagaaa acgacatatt agacatgatc
    31201 tctcaaaaaa aaaactctca tctagtaatg gataataaat cctctaagat gagagttttt
    31261 tgtcctaaaa agagctgcac caacatcttc tggcacagct ctaacagggg gcaatttatg
    31321 gaagtttgat gacttgttct gtttctttat caaagaagtg gcctttgctg atgttgaagg
    31381 caaggtcgac tatttcgcct ggtttatgga aatctcttgc atctactttt gagatgaact
    31441 cagtagaacc agtttttgtg taaagcatcg tttcagctcc aagtaattcg gaaacgacaa
    31501 cttctgattt cactacggct tcaggtgaag cttcaagggc tacttgttca ctgtggatgt
    31561 cttctggacg aataccgaag ataacttctt ttccttcata acctttttca actaatactt
    31621 tgttttttcc ttcaggaata cgaagcttaa gcccatgtcc atctgtgata acaccatttt
    31681 tcaatgtcac attaaagaag ttcatggcag gagatccaat aaagccggca acgaacatat
    31741 tgacgggtgt atcataaact tctttcggtg taccgatttg ttggataaat ccgtctttca
    31801 tgataacgat tcggtcagcc atagtcattg cttctgtctg atcgtgagta acatagatgg
    31861 tcgttgtttc aagacgctgg tgaagtttgg caatctcagc acgcatggct acacgtaatt
    31921 tagcatctaa atttgataat ggctcatcca ttaagaagac ttttgcatcc cgaacgattg
    31981 cccgtcccaa tgccacacgt tggcgttgtc caccagataa agcagctggt tttcgtttca
    32041 agtattctgt cagacctaag atctctgctg cattgtccac acgttttttg atgtctgctt
    32101 tatcatattt acgtaatttc aagccaaaag ccatattatc aaaaacagtc atatgaggat
    32161 acaatgcgta gttttggaaa accatggcaa tatcacggtc tttaggtgcc acgtcattca
    32221 tgacttttcc gccgatattt agttctcctt cagagatatc ttccagtccc gcgatcattc
    32281 gcaaagtagt tgactttcca caaccggaag gaccaacaaa aacgataaat tcacggtctt
    32341 tgattgatag attaaaatca gttacagaat aattttctgc attgtcatat tttttatata
    32401 cgtttttcag agccatttct accataatac gttccacact ttctttttta gttttattaa
    32461 ctatttaaag tataaatgaa agcgttaact aacttcaatg tcatgttgaa caagaatttt
    32521 ttcttttttt tgtcaatgtg ctagttttaa gctttaataa atatccataa gaaataaaaa
    32581 ctgatacact ggagtaaata agtatgttaa caatatcaaa aggagtgtct cttatgaaaa
    32641 tcgctttgat cgctcatgac cgaaaaaaac ctatgatggt gaaattagca acagcgtata
    32701 aaacaatatt ggaaaaacat gaactttttg ctaccgggac gactgggcag aggatcacgg
    32761 aagcaaccgg cttgccgatc catcggttta aatccggacc gttaggcggg gatcagcaaa
    32821 ttggagccat gatttcggag gatcagttag atttggtgat ttttcttcga gacccgctgg
    32881 ctgcacagcc ccatgaacca gatgtaacag cacttatccg tttatctgat gtctatgaga
    32941 ttccgttagc tacgaatatc ggaacagctg aggtgctact aagaggactg gaagccggct
    33001 tcctagattt tcgaagaatc gtccatgaaa tagacaagca tccactttca ttttgatgga
    33061 taagcgtatg gaaaaataac gtttaatgtt cgcctatatg tagctataaa agctaaacaa
    33121 actgataata tgcgatataa tacgaacgta ttggataata taaacaataa tattagtttt
    33181 tcgtatttgc agtaaatgtg aaaatatgct atagtttctg tgggaaaaga tcccaaaaat
    33241 aatacagagt agaaaataaa gcgttaagtg ctaagaggat gggatgttgc ctcttggacg
    33301 aaggacgtaa tgttcgcggt tttattttcg cattcgctgc atgatacgat gtagcagctc
    33361 ttaaaggaga tgctaaaaat gaagaaacac cggttagacg cacgtatgat tgctattatg
    33421 ggcttattga ttgcattgat ggtcactttg tcccgactgg tagcaatcga gaccccattt
    33481 attaaaatca gtgtcacatt tattccacaa gtgatcatgg gcattctttt tggtcctttt
    33541 tggtcaggga taggcgcagt tttagctgat ttagtaggaa tggccctctt ctcgaaatct
    33601 gcattcttta ttggatttac attgaatgca tttattgaag gagcaatcta cggattcttt
    33661 ttctatcgaa aagagatcac ttggaaaaat gccattctgg ctacgcttag tgtcacgctg
    33721 atcatcaact tatttttgac accattatgg cttgcgttga tgtaccatgt gccattgttc
    33781 agctgggtcg tttgggcgcc ccgcctgtta aaaacagtaa tatggttacc gattcaatca
    33841 attgccattt attacgtagg acgtagtatt ccttacaaaa aaattttaag aagtttagcg
    33901 atccacgcta aataaaaaac aagcagtctt cccatcccag aagactgctt gtttttattg
    33961 tgctgtgaat tgtgcttgat agagccgtcg gtaataacca tctttttgaa gcagctcatc
    34021 atgggtgccc atttcgacaa tagctccctg atccatcact aatatctggt ccgcattttt
    34081 gattgtggac aggcgatggg caatcacaaa actggttttt ccttccatca ttgctaagaa
    34141 ggcttgttgg atttttttct ctgtcaatgt atcgactgag ctggtagctt catctaagat
    34201 caacataggg ggattactga tcatcgttcg agcaatcgtc aacagttggc gttgtccatc
    34261 tgatatcttt aacccttgac cgccaatctt agtatctaat ttttccggca aacgttctac
    34321 aaattcatac atataagagg cttttagggc atcattgatt tctgcgtcac ttgcttccgg
    34381 attcccaaaa cgaagatttt ctcgcaatgt actgtcaaat agccaagtat cttgtaacac
    34441 cattccaaaa cttttacgta atgtatcacg ttggatagag cggatgtcat ggtcatcgac
    34501 tgtaattgtt ccttttgttg tgtcataaaa acgcatcagc aaattaacaa gtgtggattt
    34561 tccggcccct gtttttccta ctatagcaat ggtttgacct ggataagcag taaaattgaa
    34621 atcttcgatc aaacgctgct tggggtcata agaaaaagaa acatgatcaa aagagacttt
    34681 tcctttgata tttttgggtt ccaaggcata ggcatctggc aaatcaggct tttcttctgg
    34741 ctgatcgatc aaatcaaatg ctcgttctag accagaaaaa gcggtttgca gttgtgtcgt
    34801 gattccagaa agttcgataa atggtttaga aaactgactt gagtagatcg taaagctgga
    34861 aatcacacca acagtgattg acgaaccact cttcaatgcc aataggccgc caactaaacc
    34921 aatcgataaa taagcaaggt gatctacaaa acgagaagac gggtttgtca aggaagaaga
    34981 aaattgtgcc cgttgtcctt tttcatatag atcagcattt agcagttcaa attgctgttg
    35041 actagcttct tcttgctgaa aagctttcac gattttctgg ttaccgattc gttcggtcac
    35101 aaagcccgag atatccccaa taatcctttg ctgtctatcg aaatttgttt gtgaagcacg
    35161 agcaacgatc caactcacaa ggaaaataat tggtgtagaa caaagaacga ccaatgtcag
    35221 aagtggactt agacgcatca taaaaataaa cgaaagaatg atgattgcca ctccagaaaa
    35281 taattgattg aaggccgcag aaattgcaat ggaaatattg tccatatcat ttgtaaatcg
    35341 actgacgata tttccgtggg agttttgatc ataataacga agtggcaatc gattcagatg
    35401 agcaaatgca tcttttctta attttgcaac ggataagtaa gctaaccgat tgcttaacac
    35461 ttggatgagc cattgactga ctacgttgat taaaacgata ccaccgaata gaataagtaa
    35521 atggaacagt cgattgaagc ggacctgtcc ttctccaatc atttgatcga tactttgacc
    35581 aatttgtaac gtcatcacaa ctgttgcaat cccattgatg attcctagta tcacagctag
    35641 ccaaatgtct tttgcatagg cagtaaggta aggaatgaat cggcggagcg tctgcaagga
    35701 taattttttt actttcataa ctattcttcc tcctgtgatg cgacgatttc ctgatattct
    35761 gctgattgag caagtaattc ttcatgggta ccctgagcag caatcttacc ttgatccatc
    35821 actaagatat gatgtgcttc ttgaatagag cggacacgtt gggagataat aatcactgta
    35881 ctgccaagat aagtctgtag cgccttacgc aaatccaagt ctgtttgata atccaacgca
    35941 cttaaagagt catctaaaat cagtaatgct ggcttggcaa tcaaagctct tgcaatcgtc
    36001 agtcgctgtt tttgtccgcc agagaaattt ttcccatttt ccatgacttt tgtatctaaa
    36061 ccatttggaa gctgacggac aaattcttct gcctgagcga ttttcagagc ttcccaacat
    36121 tctttttctg tggcgtcttt cttaccccat tgaaggtttt cacgaatagt cccggtaaat
    36181 agtacagaag tttggggaac aagagcaatt tgctgacgga gatgatccag ctgccaagaa
    36241 cgaacatcca ttccgtccat tataactgta ccggaactga catcatagaa acgcggaatc
    36301 aattgtgtca aagtcgtttt cccgcttccg gttggaccaa tgattccaag gactgtattt
    36361 cgcttcagct gaaatgtaat attttggaga gaaagaccag aatcaggagt ataacggaag
    36421 tcaacatggt caaaagcgat aattgcttca gaagaaaaat cgggagtagt actgacctca
    36481 gcttcaagaa tcgaattttc tgtgtctagc acctctttca cccgatttcc tgaagcttcg
    36541 gcacgtgtga agatcacgac gaggtttgaa acaacgatca gtgccaaaag catttgattc
    36601 atataattga tcaaagcaag aacttgtcct tgttgcaagc taccaatatt tactttgatc
    36661 ccgccaactg tcagcaaaca gatgatcccg acattcatga tcaaggttgt agcaggtgat
    36721 aatagggcag agatattgga aacacgtaaa taattgttcg ctagatcatt tgtgctttta
    36781 tcgaacttct ctgtttcttt ttttgtctgc gcaaatgcgc ggatgacacg aacaccgcta
    36841 agattctgac taacgtgttc attgagctga tctagttttt cttgcacttt ttggtagaga
    36901 ggaacggttt ttttgatgat aaaaaataaa atgattgaga aaataggcaa taatgctaag
    36961 aaaaacagac ctacttccca gtcgatcaca aaagccatga ccactgaccc gatacttaaa
    37021 aacggtgctc tgatcaataa acgaatgacc attgctaaag ctagctgaag ctgattgatg
    37081 tcgtttgtca ttcgggtgat caaggtatct gttccaaaat gattcaactc gttgtgagag
    37141 aaagtattga tttttttgat tagctgatta cgtaactctg taccaaatcc ttgtgaagct
    37201 actgaagcat agtattggca aatgatagca caaatcagac caatcacaga catagcgacc
    37261 atccataagc ccatttcaat gatttttcct cggtcattca tttttagtcc attatcgatc
    37321 aaggaagcca tcattaaagg caggacaagt tcgaagacag cttcaagaaa tttgaagaac
    37381 gggcccaaaa tgatttgctt acgatagtct tttgcataac gtaataactg aatcatatga
    37441 ttcctcctat tatttatttg tccacaattg aaaaaacaag tactaccaca ttgtagctta
    37501 ggaaatttag aatgaaaaga gaacaagcct tttcataaat gaaagtttta ttatgaagaa
    37561 gctttagaaa ttgaatagac tatttttttt aaaaaaaaga aaatgagaaa acatgccagt
    37621 ctcttcattc ataggaacgt ctattttact taatttttct ataaatgaat tctgc
//
LOCUS       DAJSBL010000026        32176 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.026, whole genome shotgun sequence.
ACCESSION   DAJSBL010000026 DAJSBL010000000
VERSION     DAJSBL010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32176
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.026"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(407..766)
                     /locus_tag="ONT85_001501"
     CDS             complement(407..766)
                     /locus_tag="ONT85_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HCR2378483.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            complement(796..1137)
                     /locus_tag="ONT85_001502"
     CDS             complement(796..1137)
                     /locus_tag="ONT85_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2378484.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERVDQRPSGKLIQHTLVTKENTEEIQKKFAEYLMLEAK
                     KRRIKSIFAQF"
     gene            complement(1134..1808)
                     /locus_tag="ONT85_001503"
     CDS             complement(1134..1808)
                     /locus_tag="ONT85_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HCR2378485.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     KFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTAEICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIAYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            complement(2984..4282)
                     /gene="asnS"
                     /locus_tag="ONT85_001504"
     CDS             complement(2984..4282)
                     /gene="asnS"
                     /locus_tag="ONT85_001504"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HCR2378486.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            complement(4322..5512)
                     /locus_tag="ONT85_001505"
     CDS             complement(4322..5512)
                     /locus_tag="ONT85_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HCR2378487.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNPVIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            complement(5533..6060)
                     /locus_tag="ONT85_001506"
     CDS             complement(5533..6060)
                     /locus_tag="ONT85_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HCR2378488.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEETDHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            complement(6107..8896)
                     /locus_tag="ONT85_001507"
     CDS             complement(6107..8896)
                     /locus_tag="ONT85_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HCR2378489.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLVQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            complement(9043..9243)
                     /locus_tag="ONT85_001508"
     CDS             complement(9043..9243)
                     /locus_tag="ONT85_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HCR2378490.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            complement(9696..10016)
                     /locus_tag="ONT85_001509"
     CDS             complement(9696..10016)
                     /locus_tag="ONT85_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2378491.1"
                     /translation="MKKRLLWCLLSLSLSLTSQPVFAEETQRFPITGTRLYKNVYVQT
                     PDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            complement(10519..11637)
                     /locus_tag="ONT85_001510"
     CDS             complement(10519..11637)
                     /locus_tag="ONT85_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2378492.1"
                     /translation="MAKKDKELLSQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            12108..13415
                     /locus_tag="ONT85_001511"
     CDS             12108..13415
                     /locus_tag="ONT85_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HCR2378493.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWDDKVSISDYAENLSVT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETARLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAE
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
     gene            13536..14735
                     /locus_tag="ONT85_001512"
     CDS             13536..14735
                     /locus_tag="ONT85_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HCR2378494.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYSSTWTFAVI
                     LTYFVVLEIFYSTFYFLIPLVFFSIFAFSYFSEKRRLLNGLLFNIFLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVFLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCIPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYWAQSRATLNPPILLMSGGQGADEKVPE
                     AMAMKQYAMEQGIPERDILVETNSTTTLENMRYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYARQAKLKANGIGAKTAFYYLPNAFLREYIAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(14762..16030)
                     /locus_tag="ONT85_001513"
     CDS             complement(14762..16030)
                     /locus_tag="ONT85_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HCR2378495.1"
                     /translation="MEKQLPSPVRILMYMLKWLVVTAILGVFMGSLSAFFLNSLTFVT
                     DIRLAHPWLLYLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNIAHLFRLDKAEREILVISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVWRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHAYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAVLA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAAYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRIKEKNKV"
     gene            complement(16200..16841)
                     /locus_tag="ONT85_001514"
     CDS             complement(16200..16841)
                     /locus_tag="ONT85_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G-binding protein"
                     /protein_id="HCR2378496.1"
                     /translation="MEQTMKPYQYFFIKREVEQLLNAYSSVNDSKTVQTVQALAAEKI
                     RDVLGYEPSEIDTFLTTVLDVKLTKTQAERALEELKQIVQPFMQPSKPQIEKLFRKVK
                     KLKQPAWNEIDLQAHTYIGWNDPGTQKKFMVYYEDGKLKGISGTLSPTINKGICAICQ
                     KTSNVSLFLSTTKAGSDGTYTKKGNYICHDSDQCNQQLTKLAPFYEFAERVKK"
     gene            complement(17250..17753)
                     /locus_tag="ONT85_001515"
     CDS             complement(17250..17753)
                     /locus_tag="ONT85_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HCR2378497.1"
                     /translation="MTEKWREDEEYMSYVEDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TDLSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(17876..18640)
                     /locus_tag="ONT85_001516"
     CDS             complement(17876..18640)
                     /locus_tag="ONT85_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HCR2378498.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKEEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAANKEISTFLVSDEVMDAVSDLPTPQGILAVMALTAE
                     GTPDFSGGWLLLDNVQDPGNVGTMIRTADAFDMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLTEVIPLMKDKGFTVYGTELNEEAHALDKVEKSDNFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            18748..19023
                     /locus_tag="ONT85_001517"
     CDS             18748..19023
                     /locus_tag="ONT85_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HCR2378499.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            19583..20533
                     /gene="yidC"
                     /locus_tag="ONT85_001518"
     CDS             19583..20533
                     /gene="yidC"
                     /locus_tag="ONT85_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HCR2378500.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVVIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     KDVTESAKPANNKQATKQIKQPKNSTNNKGRNAGKQQKRK"
     gene            complement(20579..20980)
                     /locus_tag="ONT85_001519"
     CDS             complement(20579..20980)
                     /locus_tag="ONT85_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HCR2378501.1"
                     /translation="MSRQLVLISHGKFCEELKKSTEMIMGPQEDIYTLPLLPEDGTET
                     YKQKFLTVTDSLNDFVVLCDLLGGTPANIISKLIMEGQQITLYAGMNLPMVIEFINSQ
                     MIGSEPEFVRAGCESIVSVNELINGIAEEEE"
     gene            complement(20994..21803)
                     /locus_tag="ONT85_001520"
     CDS             complement(20994..21803)
                     /locus_tag="ONT85_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HCR2378502.1"
                     /translation="MTKSNYKLTKEDFKQINRRSLFTFQLGWNYERMQGSGYLYTILP
                     QLRKIYGDNTPELKEMMKTHTQFFNTSNFFNTIITGIDLAMEEKEGIASKETVSGLKA
                     GLMGSFAAIGDSVFVALIPTIFGALAANMATDGNPIGVFIWIFAQIVVMVFRWKQLEF
                     AYKEGISLVTTMQSRLTALTDAATLLGVFMVGALVATMINVKFSWAPTIGDVTLNMQN
                     YIDMILPKILPAGIVGGVYWLLGKKNMTPTRAIFIVLIVCVALSALGVISK"
     gene            complement(21790..22680)
                     /locus_tag="ONT85_001521"
     CDS             complement(21790..22680)
                     /locus_tag="ONT85_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCR2378503.1"
                     /translation="MDIQWWQILLLTLYAGYQILDELQIYSSMSAPVFAGLYAGLVMG
                     DLNAGLIIGGSMQLTVLGVGTFGGASKIDANSGTILATAFSISLGMNPEQAIAAIAVP
                     VASLMIQLDILARFANTYFAHRIDRHVEDMNYQAIERNFLMGALPWSLSRMIPVFLAL
                     AFGGGLVENVVSVLNGNLKWLGDGLSVAGAVLPAVGFAILLRYLPVKKHLSYLILGFT
                     ITALLATLFSNVQLLGTSVASVVKDLPATFNSLPMLAVALIGLALAMISYKNDQNKDH
                     QPLASEPSHSVEGEIEDDEI"
     gene            complement(22693..23172)
                     /locus_tag="ONT85_001522"
     CDS             complement(22693..23172)
                     /locus_tag="ONT85_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCR2378504.1"
                     /translation="MAIIGVRIDGRLIHGQVANLWTTKLNISRIMVVDDEVATNTIEK
                     SGLKLATPAGVKLSILPIEKAATNILAGKYDSQRVLIVAKKPDRILSLVEKGVPIKEV
                     NVGNMSQTNETRSITKSINVVDADIEVFKKLDQLGIHLIAQMVPNDKAEDFMNLLIK"
     gene            complement(23348..24289)
                     /locus_tag="ONT85_001523"
     CDS             complement(23348..24289)
                     /locus_tag="ONT85_001523"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose kinase"
                     /protein_id="HCR2378505.1"
                     /translation="MITTVTLNPSIDIAYTIEELNLDKVNRVETVNKTAGGKGVNVAR
                     VLNLLGEEVIATGLIGGQHGEYLKNQLDQLGIMHQFVPIKGETRDSIAILHSSRQTEL
                     LERGPFITKKEQQTFTDHFEQLLEISDIMTISGSLPQGCPLDYYKQLVALADKKGKDI
                     LLDTSGKFLLEVLEGKEKPLLIKPNLEELGELIGQRICSSIQLEKIQSLLAHPIFEGV
                     PWVVVSLGERGAVAKVAQCFYEVIIPKISVVNPVGSGDATLAGSAHALSKRQSIEEIL
                     AFSMACGMANAQQEKTGWIDPMKVTMLMKHIQVKKIK"
     gene            complement(24286..25281)
                     /gene="lacD"
                     /locus_tag="ONT85_001524"
     CDS             complement(24286..25281)
                     /gene="lacD"
                     /locus_tag="ONT85_001524"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HCR2378506.1"
                     /translation="MNEEKNRAMERLSTKDGIISALAIDQRGALKKMIKSLGKEPSED
                     QISEFKELVSAELTPYASAILLDPEYGLPAAKVRAKETGLLLAYEKTGYDATTPGRLP
                     DLLADWSSLRLKEQQADAVKFLLYYDVDEAPEINHLKHVFIERLGSECEGENRPFYLE
                     LVSYDAQISDSSSPEYAKVKPHKVIEMMKEFSDPKYKVDVLKVEVPVNMNYVNGYSTG
                     EFIHSKEEAAAFFKEQSEATPLPFIFLSGGVSTELFQQTLIFAHESGSTFNGVLCGRA
                     TWKDGVKAYVENGKDGAIHWLRTNGRENIETLNKVINETATPWFNKTKSKKVKMK"
     gene            complement(25274..26470)
                     /locus_tag="ONT85_001525"
     CDS             complement(25274..26470)
                     /locus_tag="ONT85_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HCR2378507.1"
                     /translation="MFELSKNKLKQIGAAITTQEIKQQPDLWKETIDRLMQSQENLAL
                     FFDQIKKEANGRRIRVIFTGAGTSQYIGDTLLPYLNEHGDNQFFIFHSFGTTDIVASP
                     KEFLFPNEPTLLVSFARSGNSPESVAAVQIANEQVETLFHLTITCAKEGMLAKNAQDD
                     KQNYLLLMPERANDAGFAMTGSFTCMLLSALWIFDQTAMEKKERYLEQLVKIGQDLLS
                     RDQEIQKLLPDFFERIAYLGSGSLAGATREAQLKILELTAGKVATIFDSSMGFRHGPK
                     SFLNSETLVVGFVSNQPYTRNYDLDILEEIKEDRVTANVIAIAQPGTIDFSGMTVLLE
                     DSVSKLPDAYLALAMIMTAQIIALYTSIKVANKPDTPSPTGTVNRVVKGVIIHKYETE
                     GEMTDE"
     gene            complement(26488..27216)
                     /locus_tag="ONT85_001526"
     CDS             complement(26488..27216)
                     /locus_tag="ONT85_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR2378508.1"
                     /translation="MESSNKGKSLYYQLVDLLKDRIETQMTPHDKLLSERELCQHYGV
                     SRTTVRLALQELENQGYIYRRHGKGTYVSDLSDKSSNLAGAYSFTEEMREIGRVPKTV
                     ILEFRQMEANKRIAKNLNIHLGETVFKLKRLRLADEEPLMVERSYLPAKLFMGLDLPL
                     LESKPLYDVFSQDYEQQVQLADEEFYASIVQKKDAVVLRIADGAPVLHLTRITYNVKN
                     EIIEYTDSVARADQFHYKIRHIRK"
     gene            complement(27657..28631)
                     /gene="lacD"
                     /locus_tag="ONT85_001527"
     CDS             complement(27657..28631)
                     /gene="lacD"
                     /locus_tag="ONT85_001527"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HCR2378509.1"
                     /translation="MKKMSIEKQERLKRLTDEAGIISALAIDQRGALKKMINQYKEAT
                     SEDIIQFKQMVSQYLTPYASAILLDPEYGLPAAALREKECGLLLAYEKTGYDAKMPGR
                     LPDLLNSWSVKRLKEVGADACKFLLYYDVDEPVEINVQKQIVIERIGSECEAEGIPFF
                     LELLSYDATIGDPSSLDYAKVKPHKVIEAMREFSKERYQVDVLKVEVPVNMRFVEGYG
                     QDTCYSQAEASAYFYEQSKVTEQPFIFLSAGVSAALFQDTLRFAKQSGSTFNGVLCGR
                     ATWAKGVAPFVTEGEEKASSWMLEEGKQNIKELSTLLEETATSIYVNK"
     gene            complement(28628..29086)
                     /locus_tag="ONT85_001528"
     CDS             complement(28628..29086)
                     /locus_tag="ONT85_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCR2378510.1"
                     /translation="MTIIHEENIFLQQPLDNQEEAFKFLAKQTFCLGIAHDEKEVYQK
                     LLEREKEGTTGIMSGFAIPHAKSQTINEPSLIIVTLKKGIEWNSLDRQSVDFIFALFI
                     PDSEAGTTHLKLLSLVARSLMRKEVTEQLRAAKSKIEIATILTKEIGEDI"
     gene            complement(29083..30546)
                     /locus_tag="ONT85_001529"
     CDS             complement(29083..30546)
                     /locus_tag="ONT85_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCR2378511.1"
                     /translation="MGKYQLIAATGCPTGIAHTYMAQEALEQAARKKGITIKVETHGQ
                     IGIENELTAEEIKQADAVIIAADKDVHSERFVGKRVIDVSVSTGIKDAQRLIDEALAG
                     KGTVLADNEAADTLEPEAISGANFGRSIYKNLMNGVSHMLPFVVAGGVLIAISFAVWG
                     IYSSDPDHAQYNATAAMVKAVDDAAMGMMVPVLAAYIAEGIAKRPGLVVGFVGGIVAM
                     NGGTGFLGGILAGFLAGYFILFLQKLLIILPKQLDGLKAIVLYPVIGVAFIGIVMSLL
                     AGPMKAINEGMMSFLAGFEYSNPLVLGVIVGCMCAFDMGGPVNKAAYVTGTALLTQGN
                     TTFMAGASAACIAPPLITGFATLLFGKYFDTNDRNAGLVNFILGSTHITEGAIPFAAK
                     DPIRVLPTMMLGSSIAAVLTYLFGVQVPAPHGGFLVLPVVTGKIQWVTAILIGSIVGG
                     VILGLIQKKRVTDKDKKRDNKATNRTVIEEGRAGGLS"
     gene            complement(30528..31460)
                     /gene="pfkB"
                     /locus_tag="ONT85_001530"
     CDS             complement(30528..31460)
                     /gene="pfkB"
                     /locus_tag="ONT85_001530"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HCR2378512.1"
                     /translation="MSIYTCTMNLAIDLFIETDEMHPFMVNRTKEDDIQANGKGVNVS
                     LVLKMLDFPSTALGFSAGFTGKYIEDYLQQKQIQTEFIEVPGMTRINVFTQVNQTGEE
                     YKLVNQGPEIPQTAVHNFLNQIRALQAGDYLCVSGSLPRGLSPKVLIEISRICLIIDS
                     SDQEVMDCLPYRPFLLKPNEEELSSWFGRKMVTDEDYFVYGQRLVESGAENVLLSLGE
                     KGAILFTKDRVIRGNSPKGKVVNTACAGDAMLGTFLAGYMNRRSLDETLRKSIAAGSS
                     TAFRKGLTDFLDVEELSKQIKIKEERFERWENIN"
     gene            31602..>32176
                     /locus_tag="ONT85_001531"
     CDS             31602..>32176
                     /locus_tag="ONT85_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCR2378513.1"
                     /translation="MNHKLSETEQYLWNFIEHLILEIPNYSIVKLSEQANVSTATIVR
                     TMKKKGYEGFTSFKHHLKEGENKNINFSFLDKVDKGIRRAILKNEQEVVRTINMLEIG
                     NIEDAIQRIKFADRVFVFARGFSEMIGQEMLVKLQLTGKNCQMHTDPEIIKSISRKLT
                     KKDSVIFVSLNGEKTNKNIKNKIYVFIFQFF"
BASE COUNT         9384 a         6920 c         5511 g        10361 t
ORIGIN      
        1 ttcttccata cagatgtttt ggcttattct ttctttttct cttcgatcat tgcccctgct
       61 cttatccaac ccgcacttgc cttcttgatc ttgacaggat agcaactaag tttgaagcct
      121 gttgctggca atttgtctaa attcattaat ttttcgatag gacaatatct tttttcaatc
      181 cctgcaaaat gtccttccca gatattcgaa gctccactac gctttcaaaa atatagaaag
      241 agttctttaa tgaaaattat ttcaactttc cataaacagt aatcccatat aaataaaaga
      301 cgtaaaagtc acatgggaga acttatacag gaaaacacat agagtcaggt gaaatacatg
      361 tatcccacct gactctatgc gttttctaac ctttattatt ctctttttat acaatttctg
      421 caccgaatgg catcaacgcc aatcgagcaa gtttgaaaga ttgcttggca aaaggtatcc
      481 ctatgatcgt gatacataga aggatgccgc tgacgacatg tgcgagagca attggtaggc
      541 ctgaaacgat cagccagatg atattaacga tcaatgacat gccagactgg tgataaatta
      601 cttctttgcc aaaaggcgca aaagaaaggg aggcgatctt aaaacattgc ataccgattg
      661 ggatacctat gatcgtgata caccaaagta tccccgctgc taaccagcta agaccaccga
      721 ttagtccgcc gaatataaac cagatgacat ttcctaaaca actcattgta tttctccttc
      781 tattactgtt tttgattaaa actgggcaaa aattgatttt attcttcttt ttttagcttc
      841 cagcatcaag tattctgcaa attttttttg gatttcttcc gtattttctt ttgttactaa
      901 tgtgtgttgg attagttttc ctgatggtcg ctgatccacc cgttcttctg actgttgcac
      961 gtccgcttgc gcaaatccga taatccgttt ttcttttgtt acagcaacga gcaatagttt
     1021 attcttatca tttaggtcag ctgccgtttt tgcttttacg aatgattctg gatgatcagt
     1081 aatcagctga ttgatttgag gtaaatcttc ttttactgcc caccgtattt ccatcatttc
     1141 tcacttttct ttcgctcatt tttatctcca ctaaaaaaat cgactgcggc aattgcatct
     1201 atccagcttt gtgcaatgat ctcatgtccg ccaatcgttg ggtggacccc atcttctctt
     1261 gtatatttcg aaaaaccata agcaatccct aaagcattga atatgccatc taaaggaacc
     1321 agcaatgcgc catattcctt agcaagttta cgaacaattt ggattttagg gtctaagtct
     1381 ttacgccagc catgacggtc gtcggttata ggaagtacaa acggctccat caagatgatg
     1441 ttgcagattt ccgctgtttg aagttcattt agtaattttc gataaacgga ttcaaattgc
     1501 tgcgcagatt tctcagttcc gaatatagcc tgatcgctag tattatgcca ggtatcattg
     1561 attccgatca aaatcgatac atagtcaggt tgtaaagcta aacaatcttc ttgccagcgt
     1621 acttgcagat ctgcaatttt gtctcctcct atcccacggt tcatgcattc aaatttcatc
     1681 gttggatatt gggagattag ttttccggca atcaaaagtg gataaccttt acctagatca
     1741 gttgcttctg ctcggtctct tcctgcgtct gtaatgctgt caccgataaa taatatacgt
     1801 tttttcattt gtcaaaaact cctttttgaa taaaacgatg attttcttcc ttatattatc
     1861 ccataagccc tatcaagacg aaagagaaaa tatatgtata aaaaatgaca ccagcctagt
     1921 cccttcaatc aattgaatga acgctcatca agtgtctagt tttttatact ttttttctta
     1981 acgtgccgga ttccccttga ttccttcgtt gtttggtcca gagaaatcga tcacacctaa
     2041 gcttccgtca atcagaccac tgatgacgcc atcagaacat atatttatag gctcatctaa
     2101 gacttccagt aaatgttctt ttactaatat ctcttctaga ttgcttggga tagattggtc
     2161 ataactttat ctggtccgat gatttcaagg atgaattaaa cttacatatg gatcattgca
     2221 tgctgaaaat acggacctac tccatcttcg aatgttacta ttagtttgtt ttcaggtact
     2281 agatgctgct ttatttcaac agcagaagca tcatcaaatc agatttccat ttgaataacc
     2341 ttctttgtat atgtttgatt gattgcgatt tcgttataga accttgtgaa tcaaatagca
     2401 atttcttttg ctttcttctc ttgtctgctg gaaacttcct ttatcccgta ttttaaaaaa
     2461 agaactgcga gagttcgcag ttcttttttt taaataaacg ttgctctccg gccaactcct
     2521 cggtcacaag tcacaatagt catcaaacat gaaaaactgt tttttgaaat tactccttgt
     2581 gactcggagc tgaacggcag gcgcacaacc tcttgatata aacggtgttc aatcagctga
     2641 ccctcgatat tagctaaaaa aacggtaaaa tatgagaagc tatttttccg ttttgtttct
     2701 ctaatactca ggtcaaggca ctgatttcac aacctcttcc ttaaacggtg ttcaaaagcc
     2761 agtttctcgg tattaagcca aatttatgca aaaatgtgga aagccatttc cacaaaattt
     2821 ttcttaatac tcgaaactaa acagcttttt cacaacctct ccactaaacg gtattcaaaa
     2881 gcttacatct gcggtaataa gtcccgccaa ttcaaaaaca tgaacagctg tttttgcttg
     2941 gctgttctaa ttacttgatg cagaacagct ttttcatcac ctcttatgga taaattcggt
     3001 tcaacaaacg tgggaatgga atagcttcac gtacatggtc gataccagca atccaagtga
     3061 ctgttcgttc aaggcctaga ccaaaaccag agtgcggaac agaaccgtat ttacgtagat
     3121 caagatacca tgaatattct ttttcatcca aaccgtgatt acggatttgt tctagcaaga
     3181 aatcatagtc tgttgcccgt tcgcttccgc caataatttc accgtaacct tctggtgcga
     3241 tcatatctgc acaaattacc acgtcttctc ttgttggatg tggtttcatg tagaatggct
     3301 tgatggcttt tggatagttt aggataaaga caggctgttc gaatgaattc gcaatgaacg
     3361 tttcatgagg tgaaccaaag tcttcgcccc attcgatgtc atcaaatcca tttttcttca
     3421 gtaattcgat tgcatcatcg taagtaatac gagggaatgg caattttgta taagttttta
     3481 agacttcttt gtctcgtcct agtacatcta atgcatattc gcaatgatct aaaacatttt
     3541 gtactaagaa tgctacatat tgttcctgta cttctaaact ttcttcttga tgagtgaaag
     3601 ccatttctgg ttcgatcatc cagaattcga tcaaatgacg tcgtgttttt gatttttcag
     3661 cacggaaagt cggaccgaat gaaaagactt ttccgaaagc catagcagca gcttccatgt
     3721 ataattggcc actttgagat aaataagctt tttgatcaaa gtaatttgtt tcgaatagat
     3781 ccgtagtgcc ttctggtgca gaaccagtca agatcggcgg atcaattttg acaaaatgat
     3841 gttgattaaa gaattcatac gttgcacgaa tgatttcatt tctgatctgc ataatcgcat
     3901 gttgtcttga agaacgcagc cataaatgac gatgatccat caagaagtct gttccgtgtt
     3961 cttttggtgt gatcggataa tcgtggctct cgcttaccac ttccacattt gtaattccga
     4021 tttcataacc aaattttgaa cgactgtctt cacggatttc tccagttact aacacggatg
     4081 tttcttggtg tagattttta gctgtttgga aaacttcttc cgacacttcg ctttttacaa
     4141 ctacaccttg gaaaaaggct gttccgtctc ttaattgtaa aaatgcgatt tttccgcttg
     4201 atcgcttatt tgcgacccaa gcgccgattg ttactgtttc tcccacatgg tttttcgaat
     4261 cgatgatttg aatttgttcc acgtatgact ctccttttat attccttgaa tttcttcagg
     4321 attaattttg acttttactc tcaataaatt gcttgattct gcgtaccgct tcttctaacg
     4381 tagcgcgatc agtggcataa ctcatgcgga cattttctgg tgccccaaag ccttcgccag
     4441 tcacaagcgc tacccctgtt tcttctaaaa gatcttctac ccattgtgtg acattttcat
     4501 acttacacat gcgtagcgtt tcttttacat taggaaaaag atagaaagct ccttgcggct
     4561 tttcaagaga aacacccggc agttctgcca caagcggata taacgtattc aggcgttctt
     4621 caaaggcgtt ccgcatttct tctactgtat cttgctcacc tttcaatgct tcaacagctg
     4681 catattgact gactgctgtc ggattgctag ttgattggga agcaacagcg atcattccat
     4741 tgatgatcac aggatttccc accgcataac caatgcgcca gcctgtcatc gcatatgttt
     4801 tagacacgcc gttgatgatg atggtctgat ttttgattgc ttctgaaatc gttgcaattg
     4861 gagtaaactc attgccatta taaactaaac ggccataaat atcatcagca actatcaaaa
     4921 tatcattttt tactgcccat tcaccgattg cctgcagttc attcttgcta taaatcattc
     4981 cagtcggatt cgatggagaa ttgatgatta cggcttttgt tttttctgaa cgtgcttgtt
     5041 ctagctgttc aacagtaact ttccagctat tggattcttc ccctttcaca aagacaggaa
     5101 gtccttctgc cagttttact tgctcaccgt aactgaccca gtaaggcaca ggaatgatga
     5161 cttcatcttg cgggtcaagg atcgtttgga aaagggtata caaagcaaat ttcgctccgt
     5221 ctgttacgac tgtttgtgaa ggttcataga caagtccata gtttttcttc agataatcaa
     5281 cgattgcctg tcgaagctct gggattcctg ctgttggtgt ataatagcta gctttcccat
     5341 tgcggattgc ttcgattgct gcttcttgga tgttttctgg tgtcgcaaaa tccggttctc
     5401 ccacagtcaa actcagcaca tctctgcctt ttgcttttaa tgccttggcc tttgccgcag
     5461 ctgccaaagt gacagacggt tctaatcgtt ttgttctttt tgataactcc atcccattca
     5521 ttcctttacc ttttaaattc ccttgattgt cttgacttca tctccagatt caaagccaat
     5581 caggtaataa ttcagtttcc catttgtttc ttttcctact acttcccaca caggctgatc
     5641 atcatacatc cctaaagcag ctttttcgat ttggatttca ggatgggccg cagcaattgt
     5701 ctgttttgct tcgttttcag tcagtccctt tgtttgatcc aatactttta ctttttcacc
     5761 agatttagga atgataacgg cgatttcttt tcctttatca tttgtacctg tcagtgaaaa
     5821 gtatgtcttc tttcgagtaa accaataaaa atgatcggtt tcctcaagat ttgcatattt
     5881 ctttgcgatt tctactgctt ctttcttcgc ctgcaccgcc ggacgactgg aacgcaagta
     5941 aaaaatcgag ctgatcacga tgaccgctaa taaaaatacg atcaagccaa gtaacacgcg
     6001 attaatcgtt ttttcctttg tctgtttttt cttcaagggt cgtcgctcct ttatcttcat
     6061 tccttttcat tatagcagat tttaattatg aaaaaagaca gctcctctaa cttccttctt
     6121 catttttctt taagaattca tggatatcct gatagatttc ctccatagct agctctttga
     6181 tcggtacttt tttcggtaat gcatggatga tccgtgcgcc atattttgca gtgactagtc
     6241 ttcgatccaa aataagcaat acgccacgat ctgtctccgt ccttatcaag cgacctaatg
     6301 cttggcgtaa cttcaaagct gctttaggta cggattcctg gtaaaaagga ttgatcccct
     6361 ctgattctaa ataagcgttc ctagctttga ccatcgggcg aagcggatta tcgaagggta
     6421 aacgtgtgac tacaacgatc tgcaaagcat ctccaggcaa atctacacct tcccaaaagc
     6481 tgtcagcgcc aaacaatacg cttttttctg acaaaatgaa gcgtttcaaa agcttctccc
     6541 ggcttccacc aaacccttga gctagtattt ctcgtccttt ttctagcata gacagatgca
     6601 ttttctgata gaccttctgc aaaacatcat gagaagtgaa taacacaagt attggccgat
     6661 cttcttgttc gatcagacct gtaagtgctg tctctaaata atcgatatat gcttgattgc
     6721 tgacttgatg gacggcaggt gcctcttttg gcacatagat ccttgcctgt ttctcataat
     6781 cataaggaga ggcaatcact ttaagaggtg ctgcttcaat gccaagcctc ttagagagat
     6841 agtgtcgatt agggccgatc ttcagcgttc ctccaatata aagaatacga tcatatcgag
     6901 gataccaaac agtcgacggc aacatagccg cttgaaaatc atggagatga aaaatcatct
     6961 gctgcgctgt gttcccagga acgaaccaat ggatcaaatg atctcgccag tcttccagcc
     7021 attgatccat cagcaagtat tgcttgtcta attgatcaaa taggctatat aaacttgcta
     7081 aatcgatccg catctggttt gtccagcttt cttggataat ctcaaaagtc agacgcattt
     7141 gtgactgtag ctgctggatc tcctgataaa aaagctgcag gtgatcaatg gctgtcccag
     7201 attccagtgg cagctgatcc aacatctctt ttgtcatcat gacttcttct ggatactgtc
     7261 cgttaggttg cttgccgctc actaaactca ttaatccttc tgccatacaa atctgtgctt
     7321 cgcatagcgc agaaagttct tctcgataaa tcgataacca gcgtaaggct tcatgttctt
     7381 tcggtaaata gctttgtatt cggtcaaaca gctgatttgg ttcttctaat tgctggattt
     7441 gtttatggaa atagttaata cttaatcgtt tgtcatttac tttttcagcg atttccggca
     7501 aatgatgtac ttcatcgatc aacaaaaatg gactagctgg aagcaatggt tcttttcgct
     7561 gtgtttcttg tactaaaaac gcatgattga caatcaaaaa attactttgc ctgattttct
     7621 tttgcaagaa acgaacaaag tcttgttcgt aaaatgactg atttcttgct aaaaatccaa
     7681 tacctctgtg cgcgatatct gcaaagaatg aatggttcaa atttgttaaa tgtaattcat
     7741 ccaaatcacc agtcttcgtt tgcgtcagcc aaactaagac cgccatttga tacaaagcat
     7801 attgtttttg ttctggcggt tccacaagtg tgcctttaaa acgctgcaaa tcaatatagt
     7861 ggcgatagct tttgataatt acaggttgta tgggctgatc tagcaattga ttcagcaatg
     7921 gaatgtcttt ttctatgatc tgctgctgca aaactagcga aacggtactg atgacagcgg
     7981 gtttttcagg tgtagccaaa taactcaatg gcagcagata accgattgtt ttacccattc
     8041 ctgtagctgc ttcgatcatc aaatcttttg attcatcttt tgtgaaatgg tcgtagaccg
     8101 cattcatcag tctgttctgt tcttttcgat acatcagtgt tttaccaaac attttttctt
     8161 ttgcttgttt ttttcttgga tatgtccgct cgccataatg gacggatgtg aatagttcca
     8221 cttctttttt ctgcaaagct agcccatcga cgatttctaa tgagggatca agtggctctg
     8281 gtttttcttt catttcttca atcacatgat aaatgaaacg actcgtatcc atacctgtgt
     8341 gtctacttaa tcgcgcaatc tgctctaacg tgatgattgg caattttctc atccgttctt
     8401 cgatcagcaa aagaagttga cctgtcactt cggcatcact atctgcctga tgaggattct
     8461 catgaataaa tccaaggctt tctgacaaat cagctaagcg aaagcttggt tccgtcggca
     8521 agaaaatttg tgcaagttct actgtatcga tgcctggtat cttcaaacta ggaagtccgc
     8581 atcgcatgag ttcatggttc aaaaaattat aatcaaaata aatattatgc gcgacaaata
     8641 tggtgtctgc caaaagattg taaatcgttt gggcgacatc ttcgaaataa ggcgcttttt
     8701 ggacgcgttg attcgtgatc cctgttaaat gctggatttg ttttgaaatc ttccgccctg
     8761 gatgaatatc gatcgaaaaa cgtgaagtga tccgacctga ttcaatcatg acgcagccaa
     8821 attggatgat ccgatcttct tttggattcg tcccagtcgt ttcgatatca acgactgcat
     8881 aagttcttgg tttcaaaata tcttcctctt ctcctgcctc taatgttctt ttgaaattat
     8941 actacaaagt gcagaaaaat actctcatcg ctttttaaag aaaatagaca aaaacgccca
     9001 gacacgcaaa tgtctgggcg tttatttttt agtataggtt aattaagctt tagtaacgtt
     9061 tgttgcttga ggtccgcgtt ggccttcttc aacatcgaaa gttactgctt ggccttcttc
     9121 taaagttttg aatccgtcgc cttggatagc tgagaaatga acgaatacat cgtttccgtt
     9181 ttctgcagtg atgaatccaa atcctttttc tgagttaaac cattttactg tacctgtttc
     9241 cataataaaa atcctccttg tgtatcaaca catattttgc aattacttga atggtgtata
     9301 tctggaagat gtttatttga aacaagctac cacagattac cgaacaaaaa ctactcctat
     9361 agtatatcat accgtttaca aaaaagctag agtttttcga ctttttttat taaaaaaagt
     9421 tttctcagcg attacataaa gtgaaaaacc gaactttttt ttaaaataaa cgacatattg
     9481 ttcagttggt gtttttttgc tctgatgttc ttttttaaac taaaaaaatt attctttctg
     9541 actacttatc ggttctgttt caacggtcga ggctggggaa gattctttag taggtctgat
     9601 gttctcatgg tatttctgat agcctatgac agttaccaga agaacaaggc aaaccaatac
     9661 gaccaacgag attagttttt cttttttgct catatctata cctcataaat aacaggatga
     9721 tgtccatcat atttctgagt atgagtaaaa gcaaaagctg cagctgtaga atgtaagaaa
     9781 tgccagcaac tttcataatc acaggtcgga cagtaccaaa agtaaccaaa tcttggctgc
     9841 aaagaccgtt cttctgtttc aggaatcgca gaatcaagat ctggtgtctg tacataaaca
     9901 tttttgtaca gacgtgtgcc tgtaatagga aaccgctgtg tttcttcagc aaaaactggc
     9961 tgacttgtta aacttaggga tagagacagc aaacaccata acagtctttt tttcataaag
    10021 acctccttat cgataagttt ttcccaatta aaatcatatc tcaagaacgc ccgttattca
    10081 cattttgttc ataaatgatg ttttttgtat cataatcgtc aaaaagaggt cgtgaccacg
    10141 tcacaacctc ttttttaaac ggtgttccag aagtagcttc ttcggaaata agccgaaatt
    10201 tcacaaaaat ttgagaaata atttttgtca atctcttctt atttcttgaa gctgaccact
    10261 tctgtcacaa cctcttcctt aaacggtgtt caaaagcttg catctgcggt aataagccca
    10321 ccaaacaaaa aatatgaaga gctatttttg cttgctgttc ttattacttg atgcagaaca
    10381 gctttttcac aacctctcat acacggtgtt taatcagctg accttcggta ttagctcaaa
    10441 aaacggcaaa atatgtgaaa ctatttttcc gtttgtttct ctaatactca ggtcaaaaca
    10501 ctgatttcac aacctctttt aataacgaag aatcgctgtc agactctttt catctggcgc
    10561 atctttttga tccaaaataa acacttttcc gccattaatg atcacatcat ctgctaatgc
    10621 attcaacact tgacgccgat catattctgt ttcaggatct tcaccgaaac catcagctaa
    10681 gtttgacgta gcgataaata aatgtgacac ttttccttct ttcgcagctg gaacgatatc
    10741 aactaactga tcgatgaatt tcttatctaa gagtttattg tattcttttg attcttgagc
    10801 tgcgagttca cttgacagct tcttcgcccc ttcttcgata tctctaaagg atagctgcgc
    10861 aggagaagca gaaatcgcaa cgtctgcaca ataataggga tttttagcaa tttttttgaa
    10921 catcgtctga ttttctggta gagcaaataa ataaatcggc cattgttctg gattttcgaa
    10981 agaatccttc aagtacgtat caactgcttg gtaatagttg acccaatcga tctctacttc
    11041 ttcatctttt ggatttacgc catggaaagt tactccatct cgggctccgc ctcctccatt
    11101 agaagagaag ttcaaaccgc cacctgtcag ttcagatccc aatgcagtaa cgacatcaac
    11161 cggtgcccct tctggtagag atacttcttt tacttgtttg ttgctgactt catacaattt
    11221 catggagtcg cggttcaatc ccaataaata ataagaatag ttgaactgcc catttttgat
    11281 aatagccagt aaataaggaa gatcacttac atagtattga tcgtctaccg ggatactcaa
    11341 acgatggata taggtttggt cagcagttat aatgaccgct acactcgtag ttgcactacg
    11401 ccaaaacgaa ggatctccta acaaattttc aattttttct tggaacgggg accagtcatg
    11461 ttcggaatat tttttctcaa aacgtttctt tgcttctttc gcaaaatttt tgaatttgat
    11521 ctgatctttt tccacatctt gatgggcgac atgcgtattc agcatgatcg tgacgaatgg
    11581 tcctttagcg ccttctgaag tcaattgtga caataattct ttatcctttt ttgccataaa
    11641 cgtacccctc ctaaaaaaat cattcctttg tttctatcta aagtctacca caacactatt
    11701 tttccataca ataaaacgta tctaaaaaaa taaaacggtt tctacggttt taaatttagc
    11761 tattttttta agttatcttc ataatttcct caattattta gccccagtct tcaactattc
    11821 ttcatgaaat gctgataaac taagaaaaag aataaaagag gtggatctta tgcatttgat
    11881 tatgattttc agtttattaa gtattatcag tttatttgct ttcagcgctt cccgcttatt
    11941 agatgcggaa acacagccta ttcctgtaaa agtacgtaaa aacgaccgct aaccttccat
    12001 aacgtgcgtt cttatcctaa agttttttca gaaacattga aactctctcc cctctctgat
    12061 aaaattttaa tttgtagcga aacaaattaa ttacaaggag atcggatatg tccataaaaa
    12121 aaacacgtct agtcctatta ttggctagtc tcattctttg ccttgcttct tttctaccag
    12181 ctgttgctgt gcaagctgaa gatactttca aagtaaatgc aaaagcagcc tttgctgtag
    12241 atgcgcaaag cggtaagatt ctttatgatc aagatggaga aaaaaccatg gggatcgctt
    12301 caatcacaaa aatcatcggt ctttacatcg tccttgatca agtaaaagaa gggaaactat
    12361 catgggatga taaagtttca atttcagatt atgcagaaaa tttgagtgtc accccagatt
    12421 tatcaaatgt tcctttgcat aaagaaaaca cctacaccgt taaagaatta ttcgactctg
    12481 caatcatcca gtcagccaac gcttcaatgg ttgccttggc agaaaaaatc tctggcagcg
    12541 aagcaaaatt cactgaacga atgaaagaac aattgaaaga ttggggtatc aaagatgcaa
    12601 cgatcgttaa tgcatcagga ctgaataact cttatcttgg cgaaaaccga ccagaaggta
    12661 ctggagaaaa cgacgaaaat caaatgtctg ctcaagatgt ggctatcgtt gcccgtcatt
    12721 tgatcttgga ttttccagaa atactggatg tatctagcac tacgacacaa atgtttggcg
    12781 aaaacacgca atcaccagta gaaatggtca actggaactg gatgctccca ggattcgtga
    12841 attataaaga aggcgtggac ggattaaaaa ctggtacgac tgaacttgca ggagcctgtt
    12901 tcgtcggtac gatcactaaa aacgatcaac gaatcatcac tgtcgtattg aatgcagacg
    12961 gccatgcgga aaacccaagt gcccgtttca atgaaacagc tcgcttaatg gattattgct
    13021 acgacaattg gtcagaaaaa gagcttggaa aagccaatgc aagtatccct agtctaaaaa
    13081 ccattgatgt aaaagacgga aaagaatcaa cagtgcccgt tgtgcttaaa agtcctgtaa
    13141 aagtatgggt acggaacgat atggatactg gaaaattaac aatcacacca actcttgaca
    13201 aaaaacaagt cactgacaat gagatccaag cacctatgga taaaggaaca aaaatcggta
    13261 ccgcaacgat caccttggca gaagataaat taggttatct tgaagacaac aacgcgccat
    13321 ccacagaaat catcacggat aaaaccgtag aaaaagcaaa tattttcgtt attggctggc
    13381 gacgcgtcgc agaatttttc ggtaacttgt tttaattgaa caaaaagaaa aaagcatctt
    13441 tccatgtgga agatgttttt ttgctttttc tagtttaaat aacagctttt ttcatctatt
    13501 ttcgttatac tgtataagaa aaaggagttg aatttatgaa tctaaccaga gatctgattt
    13561 ttttggttct ctatcttatt ggggtattct ccatcgttta ttggcgaaaa cagaaaaagg
    13621 atccttcctt gttcttttac agcagtacat ggacgtttgc ggtaatcttg acttattttg
    13681 tcgtgttgga aattttttat tcgacctttt attttttgat tcccttagtg tttttcagta
    13741 tctttgcctt ttcctatttt tcagaaaagc gaaggctgtt gaatggtctt ttattcaata
    13801 ttttcctcat tagttttggt atctacttgt ttgtcttgct atacgaaaca caaaatattt
    13861 ttcttggcgg actgattgcc ttgatcacga tcccgttact gcttgtcttt ctattcggca
    13921 tttatggatt gatcgttttt cttttttgga acggtgtcac agttttacgt agagagtcgc
    13981 attcattagc taatctttta acgttgatac tagccatttt tcttacattg tttcttgttt
    14041 ttgacttttt cttgttaaaa tatttaccac aatggataaa tgctttgttt ttctgcatcc
    14101 cattgatctt gatctatcta tttatcgtat tctacaactt tttgactgtc tcattcttgt
    14161 atcagttcaa ccgccctcga tacaaccaag acttcatcgt tgtgttaggc gccgggttga
    14221 tcaatggaga aacagtttct cctcttcttg ccaagcgaat caataaggcg attgcttttt
    14281 attgggcaca aagccgtgcg acattaaatc cgcctatcct attaatgtcg ggtggacagg
    14341 gagcagacga aaaagtacca gaagcaatgg cgatgaaaca atatgccatg gaacaaggaa
    14401 ttcctgaaag agatattctt gtcgaaacca attcgacaac cactttggag aatatgcgct
    14461 attcaaagga aatcatggat cagcaaatga aaggtcccta tcgcgcgatc ttcagttcca
    14521 acaattacca cattttcaga gctggtctct atgcacgaca agcgaaactg aaggcaaatg
    14581 gcattggggc aaaaacagct ttctattact tgcctaacgc gtttttaaga gaatacatcg
    14641 ccatcttgat gatgaacaaa aaaagacata tgatcatttg cggattgctt ttcgcaggtt
    14701 ccgcattctt atcactgatc actttacttt tctaaaaaaa caaggtgtga caaaacatat
    14761 gtcacacctt gtttttttct tttattctct tgacgattgg caagaatagt attccttttt
    14821 tgacggctac ttgctgtgca gaataaatac cgctgtttcc tgaacacata aagctgatca
    14881 agcagatcat aaagaagtaa gcagcagctt gtgaaccaaa taattcgatt cccatcacaa
    14941 aacaggcaat cggtgtattt gtcgcaccag aaaatacccc gataaaacct aatccagcaa
    15001 gaaaaggaac agaaacatgg agcaaaggcg ctagtactgc gccaagcgtt gcaccaatct
    15061 caaatagtgg tgtcacttct cccccttggt accccgcacc taaagacaag accgtgaaga
    15121 ataatttccc aataaaatca taagcatgtg cctgtccatt aaatgattcc tgcagtaacg
    15181 gaagactcaa cccaaggtaa cgttgtgttt gcaaaatcaa cgctgcggct acaactactg
    15241 ctccacctat cgtatttcgc caaacaggat gaggaatcca ttgcgcatac cgcgctttta
    15301 aaaagacgat cgaacggctg aatacccaac caatcaaacc aaaagcaatc ccagcaacaa
    15361 aaactttgaa gaacagcaac atactccatt caggaatcac acccatttgg taatgtagat
    15421 gagaaacacc atagctttct gtcatgaaat ttgcaaaaag cgcagcaaag aagctaggaa
    15481 atattgcttc tgttcggact ttaccgattg ccaacacttc taatccaaat aatgttccag
    15541 ctaaaggtgt gccaaataca gaactaaaac ccgcactgat tccactgata accaaaatct
    15601 cccgttctgc tttatccaat cggaataaat gcgctatatt atcagctaat gctcccccca
    15661 tttgaactgc tgttccttca cgaccaacag atcccccaaa taaatgggta gtgatggtcc
    15721 caaacaaggt caatggaatg agccgcaaag ggatcttctc ttctcctcca ttcccttgat
    15781 cgatcactaa gttattccca cgactagaaa gaccgccatg atacgcataa agataagcaa
    15841 aaagcgcgcc gctgactggc aatagataaa gtagccaagg atgtgctagt cggatatctg
    15901 tcacgaacgt caggctattt aagaaaaaag cagacaagct ccccatgaat actccgagaa
    15961 tcgctgtgac caccagccac ttcaacatgt acatcaaaat acgcactgga ctcggtaatt
    16021 gtttttccat aaaaaattcc tcctcaaata aaaaaacgcc tactaaagaa aggggctggc
    16081 cccagacttt agtaggcgtc attagctatc ttacgttagc atttaaaggc gaacaccatc
    16141 acctattcac ttccattaaa agatagcata tttttctgta aaaaacaata aaattatcgt
    16201 tatttcttta cacgttcagc aaattcatag aaaggagcca atttcgttaa ctgttgatta
    16261 cattgatcac tatcatgaca aatataattc ccctttttcg tatacgtgcc atcagaaccg
    16321 gctttcgttg tagaaagaaa gagcgagaca ttcgatgttt tctgacagat cgcacagatt
    16381 cccttattaa tcgttggtga taaagtaccg ctgatccctt ttaattttcc atcttcgtaa
    16441 taaaccatga attttttttg cgtccctgga tcattccaac cgatgtacgt atgtgcttgc
    16501 aagtcgattt cattccaagc gggttgtttg agttttttca cttttcggaa taatttctcg
    16561 atttgtggtt tgctcggctg cataaacggc tggacgattt gcttcagctc ctccaatgct
    16621 cgttctgctt gcgtttttgt cagttttaca tctaaaactg tcgttaaaaa cgtatcgatt
    16681 tctgatggtt cgtaacctaa tacatcgcga atcttttcag ctgctagagc ctgaaccgtt
    16741 tgtaccgttt ttgagtcatt gacagatgag tacgcattta ataactgctc tacttctctt
    16801 tttataaaaa aatattgata tggtttcatt gtttgttcca tgattttaaa gtcctttctt
    16861 ctaaccaaag tgcatacttt gtagaaaaaa gtgatcttct gcaaagtatg tgttacctga
    16921 agatcccgga ctcttttaaa taagatacgt tttgcatctt atcatcaccc cctttaatgt
    16981 tcattcttct atgtacatga ttcattcgat aaataaaagt aaaccgtatc ttgagcagat
    17041 agtcaactgc aaacagaaaa aggactgtga caaaagtcgt gtcaaagtcc ctttctacaa
    17101 acaaacggtg ttccagaagt agcttcttca aaaaataagc cggaatttca caaaaatttg
    17161 agaagcaatt ttcgtaaatt ccttcttatt tcttgaagct gacccacttc tgtcacaacc
    17221 tcaatatggt ctattattgg ttttacctgt taaatcgagg attcttttcc gaagtaacgt
    17281 tgccatctct tacgcatact tgctgacata ggctgagcgg cttctttgac tgcgcagtat
    17341 ttgtctacga atgttacgat atatccttct ttgtacttag gcggtgcaat cgttgcgccc
    17401 cacatgtgct tcaaaatgat atctcgctca agatccgaca aatcggtgat cttttctgca
    17461 ttctttacag cgattcttgg atggatatac gcatgtgtgc cttcatcaaa tttcgtcgta
    17521 cgccaatcat agtagaacag atcatgcaaa agtccagcac gcgctgttgc tttagcattg
    17581 ccaccccatt tttttgcgag cagataacta tagtaagaaa cactgatgga atgctccaaa
    17641 cgagtggaat gaacatgctg tgtgtaattc gctaattttt tcactgcttc tgtttctaaa
    17701 agatcttcta cataagacat atattcttcg tcttcacgcc atttttctgt cataattatc
    17761 gatctgccac ctttttgaat ttcattttct tctgaaattg atctaagtat accaccagtg
    17821 ttcggattat tctagtcgcc ttggataaaa tagccaaagc ttaatagttc cttaatcata
    17881 aagaaaaatg ataaagaaca atccctgccg caaccccaac attcaacgat tccgcatttc
    17941 ctttcatcgg gatatatacg ttctgatcag tcaaagacaa gatttcctga cttaccccct
    18001 gaccttcgtt ccccatgatt attgcgaagt tatctgattt ttctactttg tctaaggcgt
    18061 gtgcttcttc atttaattct gtgccataca ctgtaaaccc tttgtctttc attaaaggaa
    18121 tcacttctgt caaatctttt tgcaacacag gcaaatgata attgcttccc tgcatgctgc
    18181 gcagcacctt tgtactataa atatctgccg ttcctttgcc taataaaaca ccagccatat
    18241 caaatgcatc tgccgtacgg atcatcgtcc ctacatttcc aggatcttgt acattatcta
    18301 ataacagcca tcctcctgaa aaatctggcg tgccttcagc cgttaacgcc atcactgcta
    18361 agattccttg tggtgtaggt agatccgata cagcatccat gacctcatcc gaaactagaa
    18421 aagtagaaat ctctttgttt gcggccagcc agtctcccca ttctttcatg cctcgctgtg
    18481 tcacaaaaag ttcttttatt tctgcatcag acgacgcagc ttcttcaatc agatggaacc
    18541 cttccaaaag atattcttcc ttttcttccc gatatttctt tttatgtagt ttttttaatt
    18601 ctttgattct tggatttttt gctgattgga tctctttcac attcctcacc tccccatgat
    18661 tatatcatgg aataggtgct tgagcttgtt tcatcctaaa aaaatcggta tcattaattt
    18721 ataaaatagg aaagaaaggt gacgaacatg cgcaaagtta aaatgaacgt ccaaggacgc
    18781 gtccaaggcg taggatttcg ctacatgacc aagatggtcg ctgatcaact aggcgtgact
    18841 ggaacggtaa aaaacgaaga tgacggctca gtcacgatcg aagcaatcgg caatgacgac
    18901 atcatccaga aatttattga agaagtcaag aaatctccga gcccaagtgg acgagtgcag
    18961 tatgtggatg tccaagaaga cccgatgatt gaggaacgaa agaaatttga tgtggtggga
    19021 taaggttgtg aaaaagctgg tctgcatcaa gcattaagga caggttgtga aaaatagctc
    19081 tccatatttt tcaacaggct ggacttaatg ccgtagatgc aagcttttga acaccgtcaa
    19141 taaggttgtg agaaagtcgc ttagttttga gtagtaagga gaattttgtg aaaatggctt
    19201 tccacatttt tgcataaaat ttgacttaat accgagaaac tgacttttga acaccgtcga
    19261 taaggttgtt tttgagacgc tttgcctcaa gtattaaaga taggtagtaa aaaagctgat
    19321 ctataacttg caataaattt gcttaaccca tattttccag acagaacaaa aaagttttta
    19381 gttttttaaa taagaaagaa acacttggaa tcactctttt ctttccaagt atttctttct
    19441 tgattttttg tctttttttc aaaatttttc gctacgtaaa agaacgaata tttatatgaa
    19501 atttctatga agttgattga attccatgcg cttttttagt atagttaagt ttgtgtaaaa
    19561 attatagaag aggaagtatt ctatgaataa atggaaaaac tggttattgg gatccggact
    19621 tgtcagtatc ctgctctttt tatctggctg tgtgcggatg gattcaaacg gaaaccctga
    19681 cacttcggga atcgtttatc gagtactcgt ccatcctatg ggccaagcga ttacatatct
    19741 agttgagaat ttcaattggt cttatggctg ggcagttatt gcaatgactg tgatcgtccg
    19801 tatcatcatc ttgccactag gtattagtca atcgaaaaaa acaatgatcc aatctgaaaa
    19861 aatgcaagcg ttaaaaccac aagttgaagc agcccaacaa aaattgaaag ctgcaacaac
    19921 acgtgaagaa caaatggcag cacaagcaga aatgcagcaa gtataccgcg aaaacggatt
    19981 gagtatgacc ggcggaatcg gctgtctgcc tctattgatc caaatgccga tcttctctgc
    20041 cttgtacttt acggcacgtt atacagaagg tattcgggaa tcatctttct acggcatcga
    20101 tttaggctca ccaagtatgg tacttgttgt cattgcaggg gtcgcctatc tgcttcaagg
    20161 atacatctca acgatcggta ttcctgaaga acagaaaaag acgatgcgta cgatgctaat
    20221 cgtctctcct gctatgatcg tcttcatgtc gatcagtgcg ccagccggtg ttacacttta
    20281 ctgggtagtc ggtggtgtgt tcagctgtct gcaaacattc atcacaaacg ttatgatgaa
    20341 accacgtttg aaagcacaag tggcagaaga gttgaaacaa aacccgccaa aacaagtcgt
    20401 cactccaaga aaagacgtga cagaatcagc aaagccagct aacaacaaac aagcaacaaa
    20461 acaaatcaaa caaccaaaaa acagtacaaa caacaaaggc cgcaacgcag gaaagcagca
    20521 gaagcggaaa taaaaacata ggacaaaaaa gatgagagct aagccctcat cttttttgtt
    20581 actcttcctc ttctgctatt ccattaatta attcgttgac agagacaatg ctttcacatc
    20641 ctgcacgaac aaactcgggc tctgaaccaa tcatttgtga gttgataaat tcaatgacca
    20701 tcggaagatt catcccagca taaagagtaa tctgttgtcc ttccattatt agtttagaaa
    20761 tgatatttgc aggtgtacca cctaaaagat cgcatagtac cacaaaatca tttaacgaat
    20821 ctgtgacagt gagaaacttt tgtttgtatg tttcagtgcc atcttctggt aataaaggta
    20881 acgtgtagat atcctcttga ggccccatga tcatttctgt actttttttc aattcctcac
    20941 aaaattttcc gtgactaatc aataccaatt gtctactcat ttttcgctct cccttatttc
    21001 gaaatcacac ccaatgcaga taaagcaaca cacacaatta atacgataaa aatcgctcga
    21061 gtaggagtca tgtttttctt tcctaataac caataaacac caccgacgat cccagcaggt
    21121 aaaattttag gtaagatcat atcgatatag ttttgcatat tcaatgtcac atcgccaatc
    21181 gttggtgccc atgaaaattt aacattgatc attgtcgcaa ctaatgcccc taccatgaat
    21241 acccctaata aagtggctgc gtccgttaat gcagttaagc gagattgcat agtagtaact
    21301 aaggaaattc cttctttgta tgcaaattca agttgcttcc aacgaaaaac catgacaacg
    21361 atctgagcaa aaatccatat aaacacacct atcggatttc catcagttgc catattcgcc
    21421 gccaaagccc caaaaatcgt tggaatcaaa gcaacaaata cggaatctcc aatcgctgca
    21481 aaggaaccca tcaatccagc tttcagcccc gatacggttt cctttgaagc aattccctct
    21541 ttttcttcca ttgcaagatc aatcccagta atgattgtat taaaaaaatt agaagtatta
    21601 aaaaattgcg tatgtgtttt catcatctct tttaattctg gtgtattgtc tccatatatt
    21661 tttcttagtt gtggcaagat agtatataaa tagccagaac cctgcattct ctcatagttc
    21721 caacctaatt gaaaagtgaa caaacttctt cgattgattt gcttaaaatc ttcttttgtt
    21781 aatttgtaat tagatttcgt catcttcgat ttccccctca acagaatgtg atggctcact
    21841 agctaatggt tgatggtctt tgttttgatc attcttataa ctgatcattg ctagtgctag
    21901 accaatcaat gctacagcaa gcatcggcag cgaattgaaa gttgccggaa gatctttaac
    21961 aacgcttgcc acacttgttc ctaataattg aacattagag aataacgttg ctaataatgc
    22021 cgtgatcgta aatcctaaaa taagataaga tagatgtttt tttactggta aatagcgcag
    22081 aagaatggca aacccaacag ctggtaaaac agctcctgct actgaaagtc catcgcctag
    22141 ccattttaga ttcccgttta aaacagaaac aacattttca actaatccac caccaaatgc
    22201 taaagctaaa aatacaggaa tcatacgaga gagcgaccaa ggtaaagctc ccattaagaa
    22261 attgcgttcg attgcttgat aattcatgtc ttctacgtga cggtcaatac gatgggcaaa
    22321 gtatgtgtta gcgaatctag ctaaaatatc caattggatc attaagcttg ctacaggcac
    22381 tgcaatagca gcaatcgctt gttctggatt cattccaaga gaaatcgaga aggcagttgc
    22441 taaaatcgtc ccagagttgg catctatttt tgatgcgcct ccaaacgtcc ccactcctaa
    22501 aactgtcaac tgcatactgc caccaataat caaacctgcg tttaaatcac ccattactaa
    22561 acctgcatat aaccctgcaa acactggcgc actcattgaa gaatagatct gtaattcatc
    22621 taaaatttga taacctgcat ataatgttaa taataagatc tgccaccatt gaatatccat
    22681 ggatcattcc tcctatttga ttaaaagatt cataaagtct tctgctttat cgtttggtac
    22741 catttgagca attaaatgta taccaagctg atctaatttt ttaaatactt cgatatctgc
    22801 atctaccaca ttgattgatt ttgtaatgga ccgtgtttca ttcgtttgtg acatattccc
    22861 cacatttact tctttgatag gaactccttt ttctactaaa gataagattc gatctggctt
    22921 ttttgctacg atcaatactc gttgagagtc atatttgcca gctaaaatat ttgttgctgc
    22981 tttttcgatt ggcaaaatgc ttaattttac ccctgctgga gtcgctaact tcaatccact
    23041 tttttcaatt gtgtttgtgg ctacctcatc atccacgacc atgattcgac taatattcag
    23101 tttcgttgtc caaagatttg ctacttgtcc atgtatcaaa cgtccatcga ttcttacacc
    23161 aataattgcc atcatgcatt ctccttatct attttttcta tttattgttc aattaaaact
    23221 gtgacatcat caaatatccc ttctgtttca aagataacgt tttcattttt tctttcttat
    23281 atttcatttt taagctaaga acaaattcct tcccaactaa agaaaacgct ttcttttata
    23341 tttaagttca tttgattttc ttaacttgaa tgtgtttcat caacatcgta actttcatag
    23401 ggtcgatcca gccagttttc tcttgttgcg cgtttgccat accacaagcc atagaaaatg
    23461 cgagaatctc ttcgattgat tgcctttttg ataacgcatg ggccgatcct gcaagggtcg
    23521 cgtcacctga accaacagga ttaacaactg atattttagg aataatgact tcgtaaaaac
    23581 actgagcaac ttttgccaca gcacctctct ctcctaaaga aacaacaacc catggcactc
    23641 cttcaaatat tggatgagct aataaggact gaattttttc taattgaatc gaagaacaga
    23701 tacgttggcc aatcagttct cctaattctt ccagattggg cttgataagt agtggcttct
    23761 ccttaccttc caatacttca agcaaaaatt taccagaagt atctagtaat atgtctttcc
    23821 cttttttatc agctaaagca accaattgct tatagtaatc aagagggcat ccttggggta
    23881 aactacctga gatcgtcata atatctgata tttcgaggag ctgctcgaaa tgatcggtaa
    23941 atgtctgttg ttctttcttt gttatgaagg ggcctcgttc caaaagctct gtttgtctcg
    24001 aactatgcag gatagcaata gaatcccttg tctctccttt aattggaaca aactgatgca
    24061 tgatccctaa ctgatctagc tggttcttta gatattcacc gtgctggcca cctattaaac
    24121 cagtagctat cacctcttct cctaataaat ttagaaccct cgccacattc actccttttc
    24181 ctcccgctgt tttattgact gtttcaactc gattgacttt gtcgaggttt aattcttcaa
    24241 ttgtatacgc gatatcgatg gaaggattca aggtaactgt cgtaatcatt tcatcttcac
    24301 ctttttgctt tttgttttat tgaaccaagg ggtagcggtt tcatttatta ctttatttaa
    24361 agtctcgatg ttttctctcc catttgttct taaccaatga atagctccgt cttttccatt
    24421 ttcgacgtat gctttgaccc catctttcca tgtcgcacgt ccacacaata caccattgaa
    24481 ggtggaaccc gattcatgag caaaaatcag tgtttgttga aataattctg ttgaaacacc
    24541 cccactcaaa aaaatgaatg gtaatggagt cgcttcactt tgctccttaa agaaggcagc
    24601 agcctcttct ttgctgtgaa taaattcacc tgtggaatag ccattcacat aattcatatt
    24661 gactggtact tctactttca acacatcaac tttatatttt ggatcagaaa attctttcat
    24721 catctcaata actttgtggg gcttgacttt cgcatactct ggagaagaac tatcgcttat
    24781 ttgtgcatca taagaaacta actccaaata gaagggacga ttttctcctt cgcactcact
    24841 acctaaacgt tcgatgaaca catgcttcaa atgattgatt tcgggtgctt catcaacatc
    24901 gtagtaaagt aagaatttta ctgcatccgc ttgctgttct ttcaatctga gagaagacca
    24961 atccgctaat aaatcaggta gacgaccagg cgtagttgcg tcatagccgg ttttctcata
    25021 agctaacaat aaccccgtct cttttgctct gacttttgct gctggcaatc catattccgg
    25081 atccaataaa atagcagaag catagggtgt cagctcagct gatactagtt ctttgaattc
    25141 actgatttgg tcttcacttg gctcttttcc caacgatttg atcatttttt ttagagcacc
    25201 acgctgatcg atcgctaatg cactaataat cccatctttt gttgatagtc tctccattgc
    25261 cctatttttt tcttcattca tctgtcattt ctccttcggt ttcatactta tgaataatga
    25321 cgcccttgac tactcgattg accgtgcctg ttggagatgg tgtatctggt ttattagcta
    25381 ctttgattga agtatagagt gcaatgattt gtgcagtcat aatcatcgct aatgcaagat
    25441 aggcatcggg caatttactc accgagtctt ccaatagcac agtcatacct gagaaatcaa
    25501 tagtacccgg ttgcgcaatc gcaatgacat tggcagtgac ccgatcttct tttatttcct
    25561 ctaaaatatc taagtcataa tttcgcgtat atggttgatt gctcacaaaa cctacaacga
    25621 gtgtctcgct atttaaaaaa gactttggtc catgacgaaa ccccatcgaa gaatcaaaaa
    25681 ttgtagcaac tttacctgct gttaattcta aaatttttaa ttgtgcttct cttgtcgcac
    25741 ctgccaaact tcctgaaccc agataagcga tccgttcaaa aaaatcaggt aataactttt
    25801 gaatttcttg atctcttgaa agaagatctt gaccgatttt aactaattgt tcaagatatc
    25861 tttctttttt ctccattgct gtttgatcaa agatccataa agcactcaat aacatacacg
    25921 taaaacttcc tgtcatagca aaacctgcat cgtttgctct ctctggcatc aataacagat
    25981 aattctgttt atcatcctgt gcgttttttg ccaacatccc ctctttagca caagtaatcg
    26041 tcaaatgaaa caatgtttca acttgttcat ttgcaatttg cacagccgct acactttctg
    26101 gactgttgcc actgcgagca aatgaaacta ataaagtcgg ttcatttgga aacaaaaatt
    26161 cttttggtga agctacaata tctgtcgtgc caaaactgtg gaagataaaa aactgattgt
    26221 ctccatgttc atttagatag ggtaagagcg tatccccaat atattgagac gttcctgcac
    26281 ccgtaaaaat aacacgtatt cttcttccgt ttgcttcttt ttttatttga tcaaaaaata
    26341 gagctaaatt ttcttggctt tgcatcaatc tatcgattgt ttctttccag agatctggtt
    26401 gttgttttat ttcttgtgtc gtgattgctg cacctatttg ttttaatttg ttcttcgata
    26461 gctcaaacat gttgttctcc ctctcaatca ttttcggata tgccgaattt tataatgaaa
    26521 ctggtccgct cttgctacac tatctgtata ttcgataatc tcatttttca cgttataggt
    26581 tatcctcgtc agatgtaaga cgggagcgcc atcagcgatt cgtagcacaa cagcatcttt
    26641 tttttgcacg atacttgcat aaaactcttc atcagctaat tgaacttgtt gttcataatc
    26701 ctgtgaaaac acatcataca acggtttact ttcaagtaac ggcaaatcaa gtcccataaa
    26761 tagtttcgcc ggtagataag aacgctcaac catcaatggc tcttcatctg ccaaacgcaa
    26821 tcttttaagt ttaaatacag tttcacctaa atggatgttc aaattcttag caattctctt
    26881 attagcttcc atttgtctaa attctaaaat gaccgtctta gggacgcgcc ctatttcgcg
    26941 catttcttct gtaaagctat atgctccagc taagttcgat gacttatcag acaaatcaga
    27001 gacataggta ccttttccat gtcttcggta aatataacct tgattttcca attcctgtaa
    27061 agccagacgt acggtggtac gactcacccc atagtgttga caaagttctc tttcagataa
    27121 cagcttatca tgcggagtca tctgtgtttc gatacgatcc ttcaataaat caactagttg
    27181 ataatacaat gattttcctt tatttgagct ctccataaaa atcacctctt ctttactggt
    27241 tataaccaca acacaagaat agtgaattat taatcatttg tcaaaggttt tttagaccaa
    27301 aaaagctatt ataacagctc tttaaagctt tatatcttga atagacaaag atacctcaaa
    27361 aataccagtt accaataaaa gaacagaaaa aaagacgaac cacataagcg atccgtcaaa
    27421 atggtctaac ttatcgagca gcgtgatcca cattaatccc tatgatattc ctacaactat
    27481 cgttttatta cttccaatat catttacaac aactaaaaat agcttattaa tgacaaatgt
    27541 tattattagt tttagataac ttttgcagtt ttatactttt tttctataaa atttgacaaa
    27601 cattttttta aataactaat tattttttga atctgtcact cagacaatcc tttctcttac
    27661 ttgttaacat aaatagaagt tgctgtttct tctaatagtg tacttaattc tttaatattt
    27721 tgttttcctt cttctaacat ccaactactc gctttttctt cgccttcggt aacaaaaggt
    27781 gctactccct tcgcccatgt tgctcgccca cataagacac cattgaatgt cgaacctgat
    27841 tgttttgcaa aacgcaatgt atcttgaaat agtgcagcgc tgactcccgc acttaaaaaa
    27901 ataaacggtt gttcagtcac tttcgattgt tcatagaaat acgcggatgc ctctgcttga
    27961 ctataacaag tatcttgccc atatccttcc acaaatctca tattcactgg tacttctact
    28021 ttcaatacat ccacttgata acgttctttt gaaaattctc gcatggcttc aatcacttta
    28081 tgcggtttta cttttgcata gtcaagagaa gacggatcgc caatagttgc atcgtaagaa
    28141 aggagctcta aaaagaaagg tatgccttct gcttcgcatt ctgaaccaat ccgttctata
    28201 actatttgtt tttgcacatt aatctctact ggttcatcaa cgtcatagta caataaaaat
    28261 ttacaagcat ccgcacccac ttcttttaac cttttcactg accaactatt cagtaaatct
    28321 ggtagtcggc ctggcatttt cgcatcatag ccggtttttt cataagctag caacaaacca
    28381 cattctttct ctcgaagcgc tgctgcaggc agaccatatt ctggatcgag taaaatagcg
    28441 gaagcatacg gagtcaggta ctgagaaacc atctgtttaa attggatgat atcttctgat
    28501 gtagcttctt tatattggtt gatcattttc ttcaaagcac cacgttggtc gatagctagc
    28561 gcgctaataa ttccagcttc atccgtcaat cgcttcaaac gttcctgttt ttctatactc
    28621 attttcttca tatatcttca cctatttctt tagttaatat tgtagctatt tctatttttg
    28681 attttgcagc tctcaactgt tctgtaacct cttttcgcat caatgatcgt gcaacgagag
    28741 acaataattt caaatgagtc gttcctgctt ctgaatctgg gataaataac gcaaaaataa
    28801 aatcaactga ctgtctatct aaactgttcc attcaattcc ttttttcagt gtgacgataa
    28861 tcaaactagg ttcattgatt gtttgagact tcgcatgagg gatagcaaaa cctgacatta
    28921 ttcctgtagt accttctttt tctctttcca gaagtttttg atatacttct ttttcgtcat
    28981 gagcgattcc taaacagaat gtttgctttg ctaaaaactt aaatgcttct tcttgattat
    29041 ctaatggctg ttgtaaaaaa atgttctctt catgaatgat tgtcatgata aacctcctgc
    29101 tcttccttct tcgataactg tacgatttgt tgctttatta tccctcttct tgtctttatc
    29161 agttactctt ttcttttgga tcaaacctag tatcactcca ccaacgatcg aaccaataag
    29221 aatcgctgta acccattgga tttttcccgt cactacaggt aacactaaaa aaccgccatg
    29281 tggggctggt acttggacac caaacagata ggtcaagaca gcagcaattg atgagcctaa
    29341 catcatcgtt ggtaatactc ggatcgggtc tttcgcagca aacggaatag ctccttccgt
    29401 aatatgagtt gatcctaaaa taaaattaac caaacctgca ttacgatcat tagtatcaaa
    29461 atatttacca aacaataaag tagcaaaacc agtaatcaac ggtggtgcaa tgcaagctgc
    29521 tgacgcacca gccataaaag tcgtgtttcc ttgtgtcaaa agagctgttc ctgttacata
    29581 tgccgcctta ttaaccggtc ctcccatatc aaacgcacac atacagccaa caatcacgcc
    29641 aagaacaagt ggattcgaat attcaaatcc agctaaaaag ctcatcattc cttcatttat
    29701 agccttcatt ggacctgcca gcaaagacat cacgatgcca ataaatgcta caccaataac
    29761 tggatataag acgattgcct tcaagccatc tagttgttta ggtaaaatta tcagtagctt
    29821 ttgcaaaaat aagataaaat aacctgctaa gaacccagct aaaatacctc ctaaaaatcc
    29881 agtaccgcca ttcatggcta cgattccacc aacaaatcca acgactaacc ctggacgttt
    29941 cgcgatccct tctgcaatat aagctgctaa aactggaacc atcattccca ttgctgcatc
    30001 gtcaactgct tttaccattg ccgctgtagc attgtattgt gcatgatctg gatcagaaga
    30061 atagattccc caaacggcaa atgaaatagc aatcaatact ccgccagcta ccacgaaagg
    30121 caacatatga gagacaccgt tcatcaaatt tttatagatg ctacgaccaa aattagcacc
    30181 acttatcgct tctggttcta aagtatcagc agcttcatta tctgctaaga cagtaccttt
    30241 tcctgccaat gcttcgtcaa tcagccgttg tgcatccttg attcctgtac tgacagaaac
    30301 atcaatgact cgttttccca caaatctttc tgaatggaca tctttgtctg cggcaataat
    30361 gacagcatct gcctgtttga tttcttcagc cgttaattca ttctcgatcc caatttgtcc
    30421 atgggtctcc actttgatcg tgatgccttt tttcctagct gcttgctcta atgcctcttg
    30481 cgccatgtag gtgtgcgcaa ttcctgtcgg acaaccggtt gctgcaatta gttgatattt
    30541 tcccatcttt cgaacctctc ttccttgatt ttgatttgtt tacttaattc ctccacatcc
    30601 aaaaaatcag tcagtccttt acgaaaagca gtagagctgc ctgctgctat gcttttacgt
    30661 aacgtttcat caagcgatcg tcgattcatg tatcctgcta aaaaagtgcc taacatggca
    30721 tcacctgcac atgctgtatt gacaactttt ccttttggcg agtttcctcg aatcaccctg
    30781 tctttcgtaa aaagaatcgc tcctttctca ccgagggata acaaaacatt ttctgcgcct
    30841 gactctacta gtcgttgtcc ataaacaaag taatcttcat ctgtaaccat ttttcgtccg
    30901 aaccaagaag aaagttcttc ctcatttggt ttcagcaaaa acggtcgata gggtaaacaa
    30961 tccatgactt cttgatcact actatcaata atcaaacaaa tccgactgat ttctattaaa
    31021 acctttggag acagccctct tggtaaactc ccagaaacac aaagataatc ccctgcttgt
    31081 aatgctctta tttgatttaa aaaattatga acagctgtct gcggtatttc aggtccttga
    31141 ttcactaatt tgtattcttc tcccgtttga ttgacttgtg taaatacatt gatacgcgtc
    31201 ataccaggca cttcaataaa ctctgtttgg atttgtttct gctgtaaata gtcttctata
    31261 tactttcctg tgaacccagc agaaaaacct aatgccgtac ttgggaaatc tagcatcttt
    31321 aatactagtg acacattcac cccttttccg tttgcctgaa tgtcatcctc ttttgtgcgg
    31381 tttaccataa aaggatgcat ctcatctgtt tcaataaata gatcaattgc caaattcatc
    31441 gtgcatgtgt agatagacat aaccaacgac ctccttgatt ctattatgaa atacgcgaaa
    31501 tagaaaatgt tttcttaatc gtcccaacac aaagaaaacc tttacattta taaactcgtc
    31561 gtgctttttt taactaactt ttggtgaaag aaggaaagaa tatgaatcac aaattaagtg
    31621 aaacagagca gtacctctgg aactttatcg aacacctcat cctagagatc cctaactatt
    31681 ccatcgtgaa actaagtgaa caagcaaatg tctcaacagc caccattgtg cggacaatga
    31741 aaaagaaagg ctatgaaggg tttacttcgt ttaagcatca tctaaaagaa ggagaaaaca
    31801 agaacatcaa tttttcattt ttagataaag tagacaaagg aatccgccga gccattctaa
    31861 aaaatgaaca agaagtggta cgtacaatca atatgttgga aatcgggaac attgaagatg
    31921 caatacaaag aatcaaattt gctgaccggg tttttgtctt tgcccgagga ttttcagaaa
    31981 tgattggcca agaaatgctg gtaaaacttc aattaacggg aaaaaattgt cagatgcata
    32041 ctgatccaga aattatcaaa agcatcagtc ggaaactaac taagaaagac agcgttattt
    32101 ttgtttcttt gaacggtgag aaaacaaata aaaacatcaa aaataagatt tatgtcttta
    32161 tttttcaatt ttttaa
//
LOCUS       DAJSBL010000027        32046 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.027, whole genome shotgun sequence.
ACCESSION   DAJSBL010000027 DAJSBL010000000
VERSION     DAJSBL010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32046
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.027"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..378
                     /locus_tag="ONT85_001532"
     CDS             <1..378
                     /locus_tag="ONT85_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HCR2378514.1"
                     /translation="PIEFDNDERLLLIYAFMDENSIFSTDNSTYRKVVQWESDNNLRS
                     YLSDRVFDVLEKLSKSGILKEEYDSSFDEYPSRYILISEDYKKFKRYFLKNEKKISVI
                     MEKYKAPIQADPFSNLDESDLPF"
     gene            complement(748..1362)
                     /locus_tag="ONT85_001533"
                     /pseudo
     CDS             complement(748..1362)
                     /locus_tag="ONT85_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337511.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            1602..1847
                     /locus_tag="ONT85_001534"
     CDS             1602..1847
                     /locus_tag="ONT85_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2378515.1"
                     /translation="MDFPEVVKEVQEIGATISDSRSSPMNYQDAHGIKIIDQERNVRK
                     YTTINECVINENLCRAPITKHIKNRSKAKDGRTFTTF"
     gene            1945..2166
                     /locus_tag="ONT85_001535"
     CDS             1945..2166
                     /locus_tag="ONT85_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2378516.1"
                     /translation="MEEEKIFEKRWELASVEQRARYHNLMSSYRNIDWTYKEKKYLLW
                     LCQLDVNTFETFEVILDKIKNSNEKRADL"
     gene            2488..3168
                     /locus_tag="ONT85_001536"
                     /pseudo
     CDS             2488..3168
                     /locus_tag="ONT85_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
     gene            3230..3847
                     /locus_tag="ONT85_001537"
     CDS             3230..3847
                     /locus_tag="ONT85_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2378517.1"
                     /translation="MGFQVLFGSLILTIVFLKWKTSNKKSMWSLVGIVLIIFTVTAAT
                     EANYSHKIQSYKGRLVMTKKDVTRYTSSRTGGLYDYAPLDALKYKDHLKKHEVKVGDR
                     WENASHKAYNSKIVYTVEDADEQNITLPVFNYWGTVVKVNGKKSTIENDNGLVSIKGT
                     GKRTIVEVSSTYPTTMILSLLISSITFIFLFTRYLIRCCGEMKID"
     gene            4867..6036
                     /locus_tag="ONT85_001538"
     CDS             4867..6036
                     /locus_tag="ONT85_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HCR2378518.1"
                     /translation="MPNFTTEIMETLINKGDLDELFRCHLELAVNSLLQAELTAFLDY
                     EKYDRAGFNSGNSRNGNYSRSFRTEYGELNLVIPRDRNGKFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMSEISELIEKMYGHHYTPQTISNMTKIVSEDIIAFKERSLESRYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFSLAPTESAYVWKEILQDLKDRG
                     LKEVLLVVTDGLSGINDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLV
                     YQASSKEEANNQIRFMIDKWKKQYPRVVKLLMNPAILTFYNFPPSIRRTIYSTNLIEG
                     FNKQLKKYTKRKEQFPNEESLERFLVSQFNNYNQKFLCRVHKGFKEIHDTLESMF"
     gene            6509..7063
                     /locus_tag="ONT85_001539"
     CDS             6509..7063
                     /locus_tag="ONT85_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR2378519.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGV"
     gene            complement(7236..7709)
                     /locus_tag="ONT85_001540"
     CDS             complement(7236..7709)
                     /locus_tag="ONT85_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR2378520.1"
                     /translation="MRIKPIKDGEQLLFSSEENKPLYLDYVNHNLKIIIKENNLKHIT
                     LHGFRHTHCSLLFESGASVKEVYVRLGHTDIKTTMDDIYTHIAKKQTEETAIRFADFM
                     SSTEEKQGSLQKVKSMVKNEKSRLSLLDSLLNVELSVFFLFRSVCVFETVLILFF"
     gene            7721..8257
                     /locus_tag="ONT85_001541"
     CDS             7721..8257
                     /locus_tag="ONT85_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCR2378521.1"
                     /translation="MPARIHHISIVNRFIRPTFDFYHNILGLKLLMKTINQDDHTMYH
                     LFFSDNHHRVGTELTFFEVQEGNNQFFGTNTIERTILKVPSEASLHFWEIYFETQGVC
                     HYGIESFSGRPILRFEGPDATRLALVPLREFEDIDDYFPYVTDQIPTEHAILGIDAIQ
                     LRVQYSDATERELLSVGW"
     gene            8297..8668
                     /locus_tag="ONT85_001542"
     CDS             8297..8668
                     /locus_tag="ONT85_001542"
                     /inference="COORDINATES: protein motif:HMM:NF013098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCR2378522.1"
                     /translation="MTILENQDTQFYQEVQIIQDRENPVAIEGIGSVHHVAFGVENKS
                     DLQRIDKQLQERNFINSGIKDREFFVSLYYRDPNQLLIEIATGEGNLDAKAYENQSPR
                     FEEIPLFLPQYLNFIREQVEQ"
     gene            8952..10223
                     /locus_tag="ONT85_001543"
     CDS             8952..10223
                     /locus_tag="ONT85_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HCR2378523.1"
                     /translation="MKLSTQEKQLKNVSLKIPKNKISIFTSVSGSGKSSIVFDTITQE
                     AGRQLNDTYDSFTRLFLPKYSRPEVDEIDNLSTAIVVNQKKIGGSARSTLGTITNINA
                     LFRVLFSCFATPSLGYANAYSFNDPSGMCSVCQGIGKTIAFLIGLLYFSAIDNADFVF
                     SDPFTAEVLSGIEEQAKNHGYFVLVHNVNSAEDVKTIQRNWRFEGFIVVGVRVKEFLE
                     LNNVFDGPVVYIDTHLSKETLRELEQQMNRLFVNTNDYQASTLAVEHLIHYGHEKIAF
                     LSFDYIVGEPSVIQERHQAYIDTLKKYHLTFKSHWIYNNSEFERIYQELAEFTAIVVT
                     GDYLAAKLVKFLKNKNKRVMEQLSIIGFDDITFAELMDPALTTIRLNPGNKGAIAFHQ
                     LIGLAKQEEIKNQVLLLEGELISRDSVHKIK"
     gene            10468..11493
                     /locus_tag="ONT85_001544"
     CDS             10468..11493
                     /locus_tag="ONT85_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR2378524.1"
                     /translation="MVGIRDVAKRAGVSIATVSRVLNEDPTLSVTDETKRKIAESVNF
                     YHYVKKSPVAKNLTNIALITTVSEVDELEDPYFRTIRRGIQVEAEQSKVNLKKIIRLS
                     ETPLEQDDLLKCQGVLIIGQVLSSVIEEVSAINSNVVVIDDPSIEAEVDAVYTDLTKA
                     TNQHLDRLYKKGHRNIAFIGGGRVRLDSQGNKHVSDDDYRKIAYETWMKQKGLEKYSQ
                     IYLEGWTTLDGMQAADLLLKKHAKDKLPTAIMVGNDPIAVGVYRSLQKNGLHIPEDIS
                     IVSFDNIEVAEFLTPALNTVNVNTEEIGKLAVRMVNERIKEVRNVSIKVIVSNNIIVR
                     ESEKNID"
     gene            11787..12896
                     /locus_tag="ONT85_001545"
     CDS             11787..12896
                     /locus_tag="ONT85_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HCR2378525.1"
                     /translation="MLDEFEKQNPDIKVEMETTPWDQYWVKLEAATTGGNMPDVVTMH
                     SSESYKYMSQNALMGLDDVIKENDIDMGNFTEGIADFYTLEDELYAIPKDASVVGLWY
                     NKELFDNAGVDYPDETWTWETLRQAAIDLTDESKGIYGFASHIGTETGYWPFIYQNGG
                     EVFADDNTKSGLNTPEAQEALAFYRDIALVDHASPTITELQDPDKLSRFQSGRLAMMI
                     EGNWFAPGFLKNDYLVENVASAVLPKGKVNATITNGLGWAASASTKYPEEVKKLMAFL
                     ASEDANKIQAETGASIPALKGFEDEWAASSDTIDLSAFGKMLEYGVPRPYNKDGLRAE
                     QAEVQIINRILSGEVTVEDGTAQIEKEVNAILSGN"
     gene            12979..13875
                     /locus_tag="ONT85_001546"
                     /pseudo
     CDS             12979..13875
                     /locus_tag="ONT85_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648409.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
     gene            13891..14715
                     /locus_tag="ONT85_001547"
     CDS             13891..14715
                     /locus_tag="ONT85_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCR2378526.1"
                     /translation="MKNKFFSNAPIHIMLILGGIAMALPFIWMILTSGKTLVESTQIP
                     PTIFPEKFQLSNYQQVLDLLPFMAFYINTFLMVVGRVIGSVLFSAMAAYACARLSFPG
                     KNILFSLVLIQMMIPGSIFITPQYLLAQKLGILNTIPALILPGVVSAFGTFLLRQFFL
                     GLPDELEEAAYLEGANIWTIFFKIMLPLAKSGLVALGIFTALFAFKDLMWPLIVNTSI
                     DKMPLAPGLANLQGQYATNFPQLMAGSLLAIWPIVVIFIVFQRKFIEGIATTGSKL"
     gene            14747..16057
                     /locus_tag="ONT85_001548"
     CDS             14747..16057
                     /locus_tag="ONT85_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005235059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HCR2378527.1"
                     /translation="MTKITFIGAGSTIFAKNVLGDAMLTPSLQDAHIALYDIDEKRLK
                     ESNLMLKNINKNSNENRADIVSYYDRKEALREADFVINAIQVGGYKPSTVIDFDIPKK
                     YGLRQTIGDTMGIGGIFRTLRTLPVMLDFAKEMEEVCPNAWLLNYTNPMAMLTMGVLK
                     ATSIKTVGLCHSVQVCVPELFEHLGIADQYDLNDFQWKIAGINHMAFLLEITKNGEDF
                     YPTIRQLAAEKPNPHPDSVRFELLKRFGYYITESSEHNAEYHPYFIKSKYPELIDELG
                     IPLDEYLRRCEQQINDWESMREDIVNNGELTHTRSREYASYIMDSITTGTPSVIAGNV
                     LNKGLITNLPTDVCVEVPCLVDKNGVQPTYVGELPTQLAALNRTNINVQELTVEAALT
                     LDKSKIYQAAYLDPHLSSELSLTDIKNLVDDLIEAHGDYLPEYN"
     gene            16205..17464
                     /locus_tag="ONT85_001549"
     CDS             16205..17464
                     /locus_tag="ONT85_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HCR2378528.1"
                     /translation="MKWSKKIILACIALGATISLTACGKNQEDASGKVEIEFFNQKKE
                     MTQTIQEIAKDFEAKNPDIHVKVVDVPNAGEVIKTRMLAGDVPDVINLYPQSIELKEW
                     AKAGYLEDLTEEPYLENIKNGYAQRFAIEYRVYSIPLTANVYGFYYNKTAFEEMGIQA
                     PETWAEFEKIVAEIKDQNKVPFAIAGAEGWTLNGYHQLAIATAAGGEEEANNVWRFSE
                     VNGINAESKEMQKDFNRLDLLREPKALQNNWQGAGYNDTVVTFTKGDALIMPNGSWAM
                     PMIHSQNPDFEVGTFPFPADEAGQSLTIGAGDLALSVSATSQHKEEAKRFVEYMTTPE
                     AMQKYYDVDGAPCAVEGVIENTQDSPLSGLTELAFTDRHLVWLAKDWNSENDFYTLTT
                     NYLHNGNQKAMINALNAFFNPMKADGR"
     gene            17474..18346
                     /locus_tag="ONT85_001550"
     CDS             17474..18346
                     /locus_tag="ONT85_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCR2378529.1"
                     /translation="MKIKEILNRYWAIIFVAIPIILQMIFFYFPMVQGAFYSLTNWTG
                     LTYNFDFVGLNNYKILFSDPKFIKSIGFTLIVTLCMIAGQIGFGILIARALNANIKGK
                     TFFRAWFFFPAVLSGVVVSLVFKQIFNYGLPKIGSMLGIEFLQTSLLGTTWGAIFAVI
                     FVLLWQGIAMPVIIFLAGFQSIPQEIIEAARMDGANDKQIFWNIEITYLLPSISMVFI
                     MALKGGLTAFDQIFALTGGGPSDATTSLGLLVYNYAFKNNQFGYANAIALVLFILIGI
                     VSLAQLKLSKKFEI"
     gene            18358..19188
                     /locus_tag="ONT85_001551"
     CDS             18358..19188
                     /locus_tag="ONT85_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCR2378530.1"
                     /translation="MKTSKEKNISKYLLLLLGGFMILVPLVSTLFGSFKTTPDIMNNF
                     FRFPNPATLENFRRLLADGIGHYFWNSAIITIIAVVAVTAIIPMAAYSLARKMTRGKA
                     YGLMYLFLILGIFVPFQVIMIPITVMMSKIGLANMWGLILLYLTYAVPQTLFLYVGFI
                     KLNVPISLDEAAEIDGASHFTTYFKIVFPMLKPMHATTIILNALWFWNDFMLPLLILN
                     RDSNMWTLPLFQYNYTGQYFNDYGPSFASYTVGIVTITLVYLAFQRSIIAGMSNGAVK
                     "
     gene            19228..21411
                     /locus_tag="ONT85_001552"
     CDS             19228..21411
                     /locus_tag="ONT85_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HCR2378531.1"
                     /translation="MIIFDNHTQTFHLQNKKISYLLGIEENNYLTHLYFGKKITHYSG
                     GYRYPRTDRSFSPNNANAQDRLLSLDTLPLEFPGYGHGDFREPAHNIKLGNGSRVNDF
                     RYDSYEIQKGKPSLAGLPALYTEDDDEAETLIIQLKDAISGLTLQLFYTIYQNHSVII
                     RSTQLVNNSDETVEINRLASQSIDFPNRPLELIHLNGTWARERQMTREKIHTGIKVLD
                     SKRGSSSHQQNPFVALVDAKTDEFQGEAYGFNLVYSGNHETVIQKDPFDQTRVIMGIN
                     SFNFGWQLKTGDSFQTPEVVMVYSNEGLNTLSQTYHELYNHRLIRGNYRLKERPTLIN
                     NWEATYFDFNEEKIMNIVDDAKNLGIEMFVLDDGWFGGRENDFTSLGDWVETEGKLAC
                     GLENLAKQVHDNGMKFGIWFEPEMISEDSELFRAHPDWALGIPGRDRSISRNQYVLDF
                     SRQDVRDNIYQQMTAIFDRVPIDYVKWDMNRNMTEVYSVLLDSTMQGEVAHRYMLGLY
                     EFLEKLTTNYPTILFESCSGGGGRFDAGFLYYMPQTWTSDNTDAVARLKIQYGTSLVY
                     PISSMGAHVSAVPNHQTGRETSLTIRGNAAMAGVFGYELDLTQLTSEEKAEIKEQVAF
                     YKKHRQLLQYGTFYRLESPFEGNDTAWMFVSKDQTEAMVFYFRVLAEAAHPLVTLKLA
                     GLAEEATYQMEDVTFAGDELMNLGFYIDSELHGDYATQRFYLKQI"
     gene            21472..22692
                     /locus_tag="ONT85_001553"
     CDS             21472..22692
                     /locus_tag="ONT85_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligo-1,6-glucosidase"
                     /protein_id="HCR2378532.1"
                     /translation="MFYLHAFTKKQPDLNWENEEVREEIYAMINYWLDKGLGGFRIDA
                     ILNIKKKLEYGIFESDGEDGLAFIGPWILNQSGIEVWLKEMRERTFKPHNSMTVAEAD
                     VPNERLDEYIGEDGYYSMVFDFSYTDIDVPETGEWFKDSQWTWTDMRTNIFTNQLVTQ
                     DKGWGALYLENHDQPRSINKYIPEEWINDYSKKMLATLFMLLRGTPFIYQGQELGMTN
                     IEMASLEDFDDVATHSQYKRALEYGLAPEQALKAVNKRSRDNSRTPMQWNTQKNARFS
                     TSDNVWLKVNPNYPELNAEAQLTNQQSVFNYYHQLIKLRKNSVYKDVLIYGQFLPVKD
                     KNEHILLYERQLEDKIVLVILNVTATEQEYYVDEQYQQVLINNYEDVLLDQSRKLRLR
                     PFESIVLANYGGQL"
     gene            22689..24149
                     /gene="gtfA"
                     /locus_tag="ONT85_001554"
     CDS             22689..24149
                     /gene="gtfA"
                     /locus_tag="ONT85_001554"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HCR2378533.1"
                     /translation="MKIKNEAMLITYADSLGKNMGELKQVLDKHLQGVIGGVHLLPFF
                     PSTGDRGFAPSDYTTVDPALGTWEDVEALGEDYYLMFDFMINHISRESKFFQDFKKNH
                     EQSPYKEMFIRIHEFFPENRPTQEDIDLIYKRKDKAPFQTVEFADGTTEEVWNTFGEE
                     QIDLDVTKEVVKEFIRETIKDMANHGCSLIRLDAFAYAIKKLDTNDFFVEPDIWELLD
                     EVRQEAAKYDVELLPEIHEHYSIQMKIANHDYYIYDFALPMVTLYSLYSGKSERLANW
                     LKMSPMKQFTTLDTHDGIGVVDARDLLTDEELDYTSEELYKVGANVKKVYSSANYNNL
                     DIYQINSTYYSALGDNDRSYLLARAIQVFAPGIPQIYYVGLLAGKNDIELLEATKEGR
                     NINRHYYDLAEIEEEVQRPVIQQLFALLKFRNESAAFDLDGTIEVATPSESEIVITRK
                     NKNGSDSATLTANLDTKEFIIESKDIKIFTSVALKF"
     gene            24226..25332
                     /locus_tag="ONT85_001555"
                     /pseudo
     CDS             24226..25332
                     /locus_tag="ONT85_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175341.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            25654..25863
                     /locus_tag="ONT85_001556"
     CDS             25654..25863
                     /locus_tag="ONT85_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HCR2378534.1"
                     /translation="MVVITKNELMKLGYGKYQSERIIRQAKAIMVSKSYTYYENRRLG
                     LVPVESVEEVLGMKIEVEEDFQNHG"
     gene            25856..26005
                     /locus_tag="ONT85_001557"
     CDS             25856..26005
                     /locus_tag="ONT85_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCR2378535.1"
                     /translation="MVNFKKDKNGKYHFTASLGFDKRTGKRVQKNVEGLKQLKKKKSV
                     YRTCF"
     gene            26159..26824
                     /locus_tag="ONT85_001558"
     CDS             26159..26824
                     /locus_tag="ONT85_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR2378536.1"
                     /translation="MELREIRPIDIQKWQNNLLSKYKNSYVRNMYGIFQMSLDRAVVL
                     GLLEKNPAKVIGNVKKRKTTVDFWTKEEFTKVISTFYIDDLYQHFGFICMWLLFMTVM
                     RLGEATALTWKDIDFENKNLRVNKTLYYKRAKTYEFVEPETASSIRTIELDDNTITFL
                     KAWKERQVSIGGMSFVLSYNGTPTQKYTIRHIMRRHATIVGVHIIRIHGLRHSHASLL
                     ISQ"
     gene            complement(27108..28193)
                     /gene="xerS"
                     /locus_tag="ONT85_001559"
     CDS             complement(27108..28193)
                     /gene="xerS"
                     /locus_tag="ONT85_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HCR2378537.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLDNNPMDQVATITDKTTLAARAN
                     AIEKKLFLDKDTQNYLDYIANEYKNTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VININLDDLSLDKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRIQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHSSTNTTTLYTHIDNAATIDALNAL"
     gene            28527..28760
                     /locus_tag="ONT85_001560"
     CDS             28527..28760
                     /locus_tag="ONT85_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
                     /protein_id="HCR2378538.1"
                     /translation="MAIKERKFREVGNSVVLTISKEFLKENGLNPGDTVFLDEEKLRA
                     AITKKQALSDEIDLAISQALTEYDTTFRELVDK"
     gene            28757..29164
                     /locus_tag="ONT85_001561"
     CDS             28757..29164
                     /locus_tag="ONT85_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HCR2378539.1"
                     /translation="MSIHYLEAKDIIKMNVIQIKKYSPNEPLGVKDANALEMCVGQLR
                     ASAFGEEVYPSIYEKAAILLIQLIKKHPFHNANKRTAFLATFVFLKINGQLLAIDQKD
                     AVNLAVYIATYDKDFDQLKYEVINTIKTHTQPI"
     gene            complement(30301..30819)
                     /locus_tag="ONT85_001562"
     CDS             complement(30301..30819)
                     /locus_tag="ONT85_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HCR2378540.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
     gene            31937..>32046
                     /locus_tag="ONT85_001563"
     CDS             31937..>32046
                     /locus_tag="ONT85_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR2378541.1"
                     /translation="MSKRTRRTFSQEFKQQIVNLYLAGKPRVEIIREYELT"
BASE COUNT        11043 a         4815 c         5866 g        10322 t
ORIGIN      
        1 ccaatagaat ttgacaatga tgaaagattg ctgctaatat atgcgtttat ggatgaaaat
       61 tctatctttt caactgataa ttcaacttat agaaaagtag ttcaatggga aagtgataac
      121 aatttaagga gttacttgag tgatagagta tttgatgtac tagaaaaatt aagtaaatcg
      181 ggaattttaa aggaagaata cgattcttct tttgatgagt atccatcacg ctatattttg
      241 atttcagagg actataagaa atttaaaagg tattttttaa aaaatgaaaa aaagatatct
      301 gttattatgg agaaatacaa agctccaata caggctgatc cattttccaa tttggatgaa
      361 tcagatttac cgttttaaat tttaaataga taatttcatt ttttagtagc agaggtataa
      421 acttttttaa tgtttgaatt gaaaagaaat gctaataggt cgactttatg tttctcttgt
      481 gcaatcgtaa atgcggggga ccataaaatt gatctattat cgagtgtgta taaaataaaa
      541 ttcaaatctt attagtttac ataagataca ttatactaag ttgtcaatgt taaatgcctt
      601 taaataagga cgatttatgc atcaattata tttatgttaa ctaaattctt tttaacataa
      661 atgtaatcaa cttagctaga tgaatctaaa atgattgtct gtattgatga caaagctaat
      721 taataattac gtgattcaaa aaagaaatca tacaattagt tggtcgattt ctttttttat
      781 tcggtaaatt gtttgcctag tcacattgta gtctgttgca atctttgaga tagcaacccc
      841 attctcaaga tcctgaacga tactcctata gacaaggcga cgttgaggat ctttggtgtc
      901 tgcactgtat aacttaggac gacctttata gacgccgttt gctttagcga ttttaattcc
      961 ttgtgcctgg cgtcgtttcg attcagtacg ttcttgttca gcaatcatgg ctgagattta
     1021 aatgattaag tctttgatga atttatctag gagaggattt ccaattgctt cagccatgat
     1081 cggtaaactg gtgataataa gtcgcacatt cttcttcttt agataattca ctgaatcgat
     1141 aatctcgtca taattgcgtc caagtcgatc aatggcttct acaataaaaa tatcttgctc
     1201 tctgacaaaa tctaaagctt cctggaaaat cggccgatga ctaatcgtcg ctccagattg
     1261 tttttcgata aaaatttttt cagcgccaaa ctttttcatt tcttcaattt gacgttgttc
     1321 attttgatcg atggatgaaa ctctaatata agcaattttc aaatttattc cctacttttc
     1381 tataacgttt ataatacacc ctaatcatac gttagaactc taaatttttc aatgttatat
     1441 ttttgtctat ttagggtaaa ctctaaatag acaaacggtt tttgaattct aaaagttaaa
     1501 tagtttataa tagaagaaaa agaatggggt gacgatttct tagattgaaa aggaaagata
     1561 aatattatag attattactt ttgacagctg aagcaggatg gatggatttt ccagaagttg
     1621 taaaagaagt tcaagagata ggtgcaacta tttctgatag ccgctcctct cctatgaact
     1681 atcaggatgc ccatggaatt aaaataattg atcaagagag aaatgtcaga aaatacacaa
     1741 caataaatga gtgtgtaatt aatgaaaatc tttgtagggc accgataacg aaacacatta
     1801 agaatagaag caaagcaaaa gacggaagaa cttttactac cttctaagtt tttttcatat
     1861 ttgtttgcta tacttagatc taaggaatta gactttcgct gcccattatt taagtttgag
     1921 gtagcgtctt gcgagggatc aataatggaa gaagaaaaaa tatttgaaaa acgctgggaa
     1981 ttggcttcag ttgaacaaag agcaaggtac cataatctaa tgtcctctta ccgtaatatt
     2041 gattggactt acaaagaaaa gaagtacttg ctttggttat gtcagttaga tgtaaatacc
     2101 tttgaaacat ttgaagtgat tctagataag attaaaaaca gcaatgaaaa aagagctgat
     2161 ctataatggt tcgctctttt tattttatta actctttggt tactatatgt aaaatacagc
     2221 ttcatattac aataaatttc aggatacaaa tttatttaag catatataaa tataattgaa
     2281 ataggacata aatatatgag tatgagaaag gtgattccat gtttaataaa tgggcaaacc
     2341 acccaatgaa ctcgagaggt agagataatt tgaaggagac ggacaagttc tgggaagaag
     2401 tatatagtaa aaacggttct gttgcaaagt tttccaaaaa atctatttta gtgtaaaatt
     2461 gagaaaaaag acagagagga cagagtaatg aatcatttta aaggcaaaca attcaaaaaa
     2521 gacgtcatta ttgtcgctgt tggttactac ctgcgttaca atctaagcta tcgtgaagtt
     2581 caggaattgt tatatgatcg tggaataaat gtttgtcata ctacgattta tcgttgggta
     2641 taagagtaca gcaaagtcct ctattatctt tggaagaaga aaaatagaca atccttctat
     2701 tcatggaaaa tggacgaaac ctatatcaaa attaagggac gttggcatta tctttatcgt
     2761 gcaattgatg cggacggctt aaccttagat atctggttac gaaagaaacg ggatacgcaa
     2821 gcagcctatg ctttcttaaa acgactccat aaacagtttg gtgagccgaa agcaattgtg
     2881 accgataaag caacttctct tggctccgcc tttagaaagt tacagagtgt gggtttatat
     2941 actaagacag agcaccgaac tgtgaagtat cttaacaatt taatagaaca agaccatcga
     3001 cctattaaac gacgaaataa attttatcaa agtctccgta cagcctcttc cacgattaag
     3061 ggcatggaga ccattcgagg aatatataaa aagaaccgaa gaaatggaac gctcttcggc
     3121 ttttcggtgt ctactgaaat caaggtatta atgggaataa cagcctaaga tatttggagt
     3181 tcagagaggg cgcgtttgat tttcaaactt cgcaacagaa ccgagaacct tgggttttca
     3241 agtgcttttt ggttcattaa tcttaaccat agtgttttta aagtggaaaa caagcaacaa
     3301 aaaaagtatg tggagccttg taggaatagt tcttattatt tttactgtga ctgcagcaac
     3361 agaagcaaat tattcgcata aaattcagtc gtataaaggt cgtttagtta tgaccaaaaa
     3421 agatgttaca cgttatacat cctcaagaac tggaggccta tatgactatg caccattaga
     3481 tgcattgaaa tataaagatc atttaaagaa acatgaagta aaagttggtg atcggtggga
     3541 gaatgcatct cataaagcat ataattccaa aatagtctat acagtagaag atgccgatga
     3601 gcaaaatatc acattacctg tattcaatta ttggggaaca gttgtgaaag taaatgggaa
     3661 aaagagcact atagaaaatg acaatggttt agtgtctatt aaaggtacgg ggaaaagaac
     3721 tatagttgag gtttcatcta cgtatccaac tacgatgatt ctatctttat taattagttc
     3781 aataactttt atattcttat ttacacgtta tttaataaga tgctgcggtg aaatgaaaat
     3841 agattagata gatattataa atatgttgat tctattttca tagcaatgga gaaaatacat
     3901 tagcaatata taaaagaagg tatcaagagt cacagtgaat ttattccgaa aagtgaaaag
     3961 agcaaatata aaatttaatg aaaatataga actaaatagg aaaacgtaga cgtaattttc
     4021 tacgttttcc ttttcatata tattaaatat tacggaaaat tatataaact aatttagtgt
     4081 tttgtttgta attcctaatt ttgctccact tttagataat ttttaggtct atttaactca
     4141 tcagacattc tatgctcatt tttctccaaa tttgtttcaa ctctttcgta cttaattttg
     4201 cgtatttgaa aattcttctt ttagagtact tttattacga tactagtatg atatatatct
     4261 atagcaaaca gttaaaatct tcctaaaata agcgaatagc attcctaaaa cctataaaat
     4321 ttttagatga agcgacaagt ggacaattaa taaactactt gaacatgaaa ttatggatca
     4381 cctttcaaat ttagaaaatg gaattcttat acttattgcg caacatttat tgattgcaaa
     4441 attatgtgac aaaaaaaact agtagaagaa agaataatgg cttgataata aagtactacc
     4501 tattaaaggt agtactttat ttaatttagt ataaattaac atactactat attttaggtt
     4561 tcatattata taaattaata atatactacc atttgttggt agtataattt aaaggtaata
     4621 agttattctt ttattactat gtttgggttt tatagcaaag aaagtttaat aagagtttgg
     4681 acctagcaaa caatattaat gaggtcatta aatcttatta tctttagtga tataacgaaa
     4741 tatctacaca gacaaaatga tagcgtaaaa tattttgtgt aaatagatat ttagggtaga
     4801 aaaaaagaaa gtccattctg taaaattaaa gttaccacaa ccgaaatttt ataggaggac
     4861 tttctcatgc ccaattttac tacagaaatt atggaaacac taatcaataa aggtgattta
     4921 gatgaattat ttcgatgtca tttagaatta gctgttaatt cattattgca ggctgaatta
     4981 acagcttttc ttgattatga aaaatatgat cgtgctgggt tcaattcagg taattcccgt
     5041 aatgggaatt attcacgttc attcagaaca gagtatggag aattaaattt ggtaattcct
     5101 agagatagaa atggtaagtt ttctcaacaa actttacctg cttataaaag aaccaatgac
     5161 tctttagaaa ccactattat tcagctattt caaaaaggaa ttactatgtc tgaaatctct
     5221 gaattgattg aaaaaatgta cgggcatcac tatacgccac aaaccatttc aaatatgact
     5281 aaaatagttt ctgaagatat tattgccttt aaagaaaggt ccttagaatc cagatactct
     5341 gtcattttta tggatgctac tcacattcct ttaaaaagac aaactgtttc aaaagaagca
     5401 gtttatattg taattggcat tcgattagat ggaaccaaag aggttcttgg gtttagtctt
     5461 gctccaactg aatctgctta tgtttggaaa gagattcttc aagacctaaa agaccgtggt
     5521 ttaaaagagg ttttattagt cgtaactgat ggtttaagtg gcattaacga tagtattcat
     5581 agtgtttatc caaatgctca atttcaacaa tgttgtgtcc atatatctag aaatatcgct
     5641 cataaggttc gtgttagtga tcgacaagaa atctgtagcg atttcaaatt agtttatcaa
     5701 gcaagttcaa aagaagaagc aaataatcaa ataaggttta tgatagataa atggaaaaag
     5761 cagtatccac gagtggttaa attgctcatg aatcctgcta tattaacctt ttataatttc
     5821 cctccgtcaa tcagaagaac tatttattca actaatttga ttgaaggttt taacaaacaa
     5881 ttaaagaaat ataccaaaag aaaagagcaa tttcctaatg aagaatctct ggagagattt
     5941 ctagtatctc aattcaacaa ctataatcaa aagtttctat gccgagttca taaaggtttt
     6001 aaagaaatac atgatacatt agaatcaatg ttttaacttg atcaacagaa tgggcttttc
     6061 catttacaca aaattattga cgctcccgac aaaattatga tcttgcgtaa agttataaaa
     6121 tattaataaa aatattttat taatgaaaag cttggtagca cggttattca tatgctaatt
     6181 tatttcactt tatagtaagg ggagttctta gtatattgtt caaacttaaa tttcatataa
     6241 tttaattatg taaattagct attctaagaa cttctacgga taactgcccg tctaaaattt
     6301 aaataagagc ttagaatctt catataagta aggttaattt ttttgtacaa aaaagtcgga
     6361 atattctgag ttactgaaat gatataaaat cataaactaa atcccttgat tttattgact
     6421 ttcaaaagtt tatatatata agttaatgat tatggtaaaa tatatacata atcattaact
     6481 cagaatattt ttaagaaggg aaacaatagt gagctataat gtacaaccgt taaggacaaa
     6541 tgaagagata caagattttc agttttggct aagaagaaca agtaatccgg aaagagatag
     6601 ttttttattt ttgtttggaa tcaataatgg gttacgtatg tcagatatta taggattaaa
     6661 agttggagat atacgaggca aaagtaagcc tattattgtt gaacataaaa caggtaagcg
     6721 aaaaccgatt tttatagata atttgagaga agaaatttta ttatatactg aaggtaaaga
     6781 agaaaatgat tggttatttc cgagtcggca acaaggtcga catataacaa gggatcgtgt
     6841 ctatcagata tatgcagata tagcagaaaa attaggacga gatgatatag gaacacatac
     6901 attaagaaaa acttttgggt atcactacta caagaaaact agagacattg ctaccttaat
     6961 gtttattttt aatcatagct cgcaagcaat tacaaaaagg tacattggta taactgaaga
     7021 tgaaatagga gcatcacttc gtggatttaa actaggtgta taatttaaaa atttttataa
     7081 acagtaaaga caaagattaa aagctatttt tatctagaac gattttattt cataaagttc
     7141 gcggaataaa agctggggtt actggtactt ggtataggtt cgtgttatat aaactaagta
     7201 attaaataac atttttaggt tggaaaagga tgaaattaaa aaaagagaat caatacggtc
     7261 tcaaaaacgc aaacgctgcg gaataagaaa aatactgata actcaacgtt tagcagacta
     7321 tctaataggg atagtctgct tttttcgttt ttgaccatac ttttgacctt ctgcaaactt
     7381 ccttgctttt cctctgtaga actcatgaaa tctgcaaaac gaattgccgt ttcctctgtc
     7441 tgctttttcg cgatatgggt gtatatatca tccatagtgg ttttaatatc tgtatgtcca
     7501 agtcgaacat acacttcttt gacagacgca ccagattcaa agagcagact acaatgggta
     7561 tgacgaaaac catgaagtgt gatatgtttt aagttattct ctttaatgat gattttcaaa
     7621 ttgtgattta catagtctaa ataaaggggc ttattttctt ctgatgaaaa caaaagttgt
     7681 tcaccatctt ttataggttt gattctcatt tttagatatt atgcccgcac gtattcatca
     7741 catttctatt gtgaatcgct ttattcgacc gacatttgac ttttatcata atatactcgg
     7801 tttaaaatta ctaatgaaaa caattaatca agacgatcat accatgtatc acctgttttt
     7861 ttcagataat caccaccgtg tgggaactga actgactttt tttgaagtac aagaagggaa
     7921 caaccagttt tttggaacca atacgattga acgaacgata ctaaaagttc cttccgaagc
     7981 atcattgcat ttttgggaaa tatattttga aacacaaggc gtttgccatt atggaatcga
     8041 atcattttct ggacgaccta tcttacgttt tgaagggcca gatgcaacac gattagcttt
     8101 agttccctta cgcgaatttg aagatattga tgattatttc ccgtatgtca cagaccagat
     8161 ccctacagag catgccattt taggaattga tgccattcaa ctacgcgtac aatattccga
     8221 tgcgactgaa cgagagttat tgtcggtagg ctggtagcac aaagaatacg tcagtttctt
     8281 ttcgggtcat caacgagtga caatcttaga aaatcaagac actcagttct atcaagaagt
     8341 tcagattatt caagatcgtg agaatccagt tgccattgaa gggattggta gcgtccatca
     8401 tgttgcgttt ggcgtagaaa ataaatctga tttacaacgc atcgacaaac aattacaaga
     8461 gcgcaatttt ataaattcag gaataaaaga tcgtgaattt tttgtatcac tttattatcg
     8521 tgacccaaat caattattga ttgaaattgc tacaggggaa ggaaacctag atgcaaaagc
     8581 gtatgaaaat caaagtccac gctttgaaga aattccttta tttttaccgc agtacttaaa
     8641 tttcattcgt gaacaagtgg agcaataatt agctaatcaa atccatgacg aagagtaaga
     8701 ggttgactca taagtcaaaa aataaagaaa aacgcatcaa atccattcta aagaatgttg
     8761 atttgatgcg gttcctattt ttataaacta tcattttcga ctaataaaac tacttatgag
     8821 tcagcctctc tttttttctt gatttttata cctctttcta ggtaaactaa acatctgcca
     8881 aaaataccgt gtctcattga aacattctca actatttcaa aataaagggg agtaccaaat
     8941 gaacgagtat attgaaatta tccacgcaag agaaacaact taaaaatgtc agtctcaaaa
     9001 ttcccaaaaa taaaattagt atttttacca gcgtatctgg ttcaggaaaa tcatcgattg
     9061 tttttgatac tattacccaa gaagctggtc gtcaactgaa tgacacctat gatagtttta
     9121 cccgtttatt tttacctaaa tacagtcgtc cagaggttga tgagattgat aatctttcta
     9181 cggcgattgt ggtcaatcaa aagaagattg gtggcagtgc gcgctcaacg ttagggacga
     9241 ttactaacat caatgcgtta tttcgtgtgt tgttttcatg ttttgcaaca cctagtttag
     9301 gatatgcaaa tgcttattca tttaatgatc ctagtggaat gtgttctgtc tgtcagggaa
     9361 tcggtaagac gattgcattt ttaataggat tgctttattt ttcggctatt gataatgctg
     9421 attttgtatt ttctgatcca tttaccgcag aagttttgtc tggaattgag gaacaggcaa
     9481 agaatcatgg ttattttgta ttggtgcata atgttaattc agcagaagat gttaaaacta
     9541 ttcagagaaa ttggcgtttt gaggggttca ttgttgttgg tgtacgtgta aaagaatttt
     9601 tggaattaaa taacgttttt gatggtcctg ttgtttatat tgatacgcat ctttcaaaag
     9661 agacattaag agaacttgaa caacaaatga atcgtttatt tgtaaataca aatgattacc
     9721 aagctagtac attggcagtg gaacatttga ttcattatgg tcatgagaaa atagcgtttc
     9781 tttcttttga ctacattgta ggtgaaccaa gtgttattca agaacgacat caagcttata
     9841 tcgatacatt aaagaaatat catttaacct ttaaatcgca ttggatatat aacaactctg
     9901 aatttgagcg aatttatcaa gaattagctg aatttactgc aattgttgtt actggagatt
     9961 atttagcggc taaattagta aaatttttaa aaaataagaa taaacgagtg atggaacaat
    10021 tatcgattat tggatttgat gatattactt ttgcagaatt aatggatcct gcgttgacaa
    10081 caatacgact aaatccaggt aacaaggggg cgattgcttt tcaccaactg attggactgg
    10141 ccaaacaaga agaaataaaa aatcaagtac tactattaga gggagaattg atttctcgag
    10201 actctgttca caaaatcaaa taaaaaagct ctgtgaaata agtatagtta acaataaaat
    10261 atccgaacga tagagagaat gaactgtgat ctagcagttc ctcgtcgcaa attcactaga
    10321 attagcatta tttactctaa attgttcgga tattttttct tgtttttaat tatgattaca
    10381 gtgcctattt ttaacgaaat ttgcttattt tcaaaatgta tgattatatg ttagtaaaca
    10441 ctttcaaata tgaaaagagg agctattatg gtaggaatta gggatgttgc aaaacgggcg
    10501 ggtgtgtcca ttgcaacagt atcgcgcgtg ctaaatgagg atccgacact ttcagtaacc
    10561 gatgaaacca aacgaaagat tgcagaatcg gttaattttt atcattatgt caaaaaaagt
    10621 cccgttgcaa aaaatttaac aaatattgca ttaataacga cagtttctga agtagatgag
    10681 ttggaagatc cctattttcg aacgattcgc agagggattc aagtcgaagc agaacaaagc
    10741 aaagtcaatt tgaagaaaat tattcgtttg tcagaaacac cgttggagca agacgatttg
    10801 ctaaaatgtc aaggtgtgtt gattattggg caggtgctat cttctgtgat tgaagaagta
    10861 tcagcaatta attcgaatgt cgtagtgatt gatgacccta gtattgaagc tgaagtagat
    10921 gcagtttata ctgatttaac aaaagcaacg aatcagcatt tagatcgatt gtataaaaaa
    10981 ggacaccgaa acattgcgtt tatcggagga ggacgtgttc gtttagatag ccaaggaaat
    11041 aaacacgtgt cagatgatga ttatcgaaaa atagcctatg agacgtggat gaaacaaaaa
    11101 ggattagaaa aatatagtca aatttattta gaaggctgga ccacacttga cggaatgcag
    11161 gcagcagatt tacttttgaa aaaacatgct aaagataagt tgccgactgc tatcatggta
    11221 ggaaatgatc ctattgctgt gggggtttat cgttcacttc aaaaaaatgg tctacatatt
    11281 ccagaagata tttcgattgt gagttttgat aatattgaag ttgctgaatt tttaacacct
    11341 gcattaaata cggtgaatgt gaatacagaa gaaattggaa aactagcagt tcgcatggtt
    11401 aatgaacgaa taaaagaagt tagaaatgtt tcgatcaaag tgattgtttc taataatatt
    11461 attgttagag aaagtgaaaa aaatatcgat taaaaaagta aacatccaaa aaagggtgtt
    11521 tacttttttg tttactaata tttttagtaa aaaatctcga caaaacatta agtaaacagt
    11581 tatggtgttg ttaacaaaaa gaaaacgcat tcaaaacagg aggaattaag atgaaacatt
    11641 tcaaaaaaat agctttaaca gggatagctg ttttaactgg gacattactc gcagcatgtg
    11701 gtgggggaga tagtaattct aaagacgggc aagtaacttt aagctatgcg agttgagata
    11761 ctgaccaagc tccaggacta agaaaaatgt tagatgaatt tgaaaaacaa aatcctgata
    11821 tcaaagtaga aatggagaca acgccttggg atcaatattg ggttaaatta gaagctgcaa
    11881 caacgggagg taatatgcca gatgtagtaa ccatgcactc tagcgaatcc tataaatata
    11941 tgtcacagaa tgctttgatg ggacttgatg atgttatcaa agaaaatgat attgacatgg
    12001 gaaactttac tgaaggaatt gctgatttct atacgcttga agacgaacta tacgcaatcc
    12061 ctaaagatgc ttctgtagtg gggctatggt acaacaaaga attatttgat aatgcaggtg
    12121 tcgattatcc agatgaaaca tggacatggg aaacactcag acaagctgca attgatttaa
    12181 cagatgaatc aaaaggcatc tatggttttg catcacatat tggaacagag actggctact
    12241 ggccatttat ttatcaaaat gggggagaag tatttgccga tgacaataca aaatcaggtt
    12301 tgaatacccc agaagcacaa gaagctttag ctttttatcg agatattgca cttgttgatc
    12361 atgcttcacc aacaattaca gaattacaag atcctgataa gttaagtcgt tttcaatcag
    12421 gtagattagc aatgatgatt gaaggaaact ggtttgcacc gggatttttg aaaaatgact
    12481 atctagttga aaatgttgct tctgccgtgt tacctaaggg aaaagttaat gcaacaatta
    12541 caaatggact tggttgggct gccagtgcga gtacaaaata cccagaagaa gtgaaaaaac
    12601 taatggcatt tttagctagt gaggacgcca ataaaatcca agcggaaacg ggggcatcaa
    12661 tcccagcact aaaaggcttt gaagacgagt gggctgcttc ttcagataca attgatttga
    12721 gtgcttttgg aaaaatgttg gagtatggag tccctcgtcc atacaataaa gatggattac
    12781 gagctgaaca agcagaagtt caaattatta accgtattct gtctggtgaa gtaactgttg
    12841 aagatggaac cgctcaaatt gaaaaagaag tcaatgcgat attgagcgga aattaaatta
    12901 gttaattata caatatacag gcgaaaggat tctatttcgt ctgtatatta atggatgaat
    12961 accaaaaagg gggatattat gaaaaatcga atttttggaa aatatcgtaa agatcaagtg
    13021 atatggggct atatttttat ttttccaacg gcattaggat taattttatt aaatatatgg
    13081 ccagcaattc aaacagcaat tttaagtttt caaaaaacgg tagggttcgg tgatactgaa
    13141 tgggtcggat taagaaatta ccaaaaaatg tttgcagacg gtgaagtttt tcagtcttta
    13201 ttaaacacat taatttatgc agtggtttcg atacctagta ttgttgtatt atccttgttg
    13261 gtggcagttt taatgaatag aaaagttaaa ggattgtcga tttatcggac aatttatttt
    13321 ctaccagtcg ttgctgcacc atccgcagtc gcaatgattt ggcgctggat gttcaataat
    13381 gattatggac tgattaataa catgttggct aaaataggtc tcgatggacc agcatggtta
    13441 acggatccat cgattgcgat ttattctatt atcattgtag gagtttggag tgctattggt
    13501 tataacatgg tgttattact agccggctta taagaaattc ctaaagatta ttatgaagca
    13561 gcggaaattg atggtgctag tccaatccag cagtttttca acattacgtt accattagtt
    13621 acaccaacat tattttttgt agttgtaaca acagttatca atgctttcca agtatttgat
    13681 gttattttta tgatgattac tcctaatagt accgccatgt ttaaatcaca atcattagcc
    13741 tatttattct ataaacattc atttatttta aatgacaagg gatatggttc ggcaatcgtt
    13801 atgttccttt tagtgattat attaattatt actgcaattc aaatgaagtt gcagaaaaag
    13861 tgggtaaact attaaggagg ctatcaaatt atgaaaaata aatttttttc aaatgcgcca
    13921 atccatatca tgcttatttt aggtggtatt gcaatggcat taccttttat ttggatgatt
    13981 ttaacttcag ggaaaacgtt ggttgaatct acccaaattc cacctacaat tttccctgag
    14041 aaatttcagc taagtaacta tcaacaagta cttgatttat taccattcat ggctttttat
    14101 atcaatacat ttttaatggt tgttggacga gtgattggct cggttttatt ttcagcaatg
    14161 gctgcatatg cttgtgcgcg tctaagtttt ccagggaaaa atatcctttt cagcttagta
    14221 cttattcaaa tgatgattcc cggttccatt tttattactc cacagtattt attagcacaa
    14281 aaattgggta ttttaaatac aattccagca ttaatattac caggggtagt tagtgcgttt
    14341 ggtacgtttt tattaagaca atttttctta ggtttacctg atgaattgga ggaagcggcc
    14401 tatttagaag gtgccaatat ttggacgatc tttttcaaaa ttatgttgcc gcttgctaaa
    14461 tcaggattgg ttgcacttgg aatattcaca gcgttgtttg catttaaaga tttaatgtgg
    14521 ccattgattg tcaatacttc aattgataag atgcctttag ctccgggatt agcaaactta
    14581 caaggacaat atgccacgaa cttcccacaa ttaatggcag gttcgttatt agcaatttgg
    14641 ccaatagttg tgatattcat cgttttccaa agaaaattca ttgaaggaat tgctacaaca
    14701 ggtagtaaat tataaaacta ttaattagtg attggagagt aagtacatga caaaaattac
    14761 atttattggt gcaggaagta ctatttttgc aaaaaacgta ttaggcgatg cgatgttaac
    14821 tccaagtcta caagatgcac atattgcatt gtacgatatt gatgaaaaac gactaaaaga
    14881 atcaaattta atgcttaaaa atattaataa aaattcgaat gaaaatcgtg ctgatattgt
    14941 ttcttattat gatcgcaaag aagcgttaag agaggctgat tttgttatca atgctattca
    15001 agtgggtggt tataaacctt caacagtgat tgattttgat attcctaaaa aatatgggtt
    15061 gcgacaaact attggtgaca ccatggggat tggtgggatt tttagaaccc tacggacact
    15121 accagtaatg ttagactttg ctaaagaaat ggaagaagtt tgtccaaatg cttggttatt
    15181 gaactataca aatccgatgg cgatgttaac catgggtgtt ttaaaagcga cgagtattaa
    15241 gactgttggt ttatgtcata gcgttcaagt atgcgtacca gaattgtttg agcatttagg
    15301 aatagccgat caatatgact taaatgactt tcaatggaaa attgcaggca ttaatcatat
    15361 ggcattcttg ttggaaatta caaaaaatgg ggaagatttc tatccaacaa ttcgtcagtt
    15421 agctgcagaa aaaccaaatc cacatccaga ttctgtacgt tttgaattgt tgaaacgttt
    15481 tggttactat attacagaat caagtgaaca caatgcagaa taccatccat actttattaa
    15541 atcaaaatac ccagaattga ttgacgaatt agggattccg ttggatgaat acttacgtcg
    15601 ttgtgaacaa caaattaatg attgggaaag catgcgagag gacattgtaa ataatgggga
    15661 gttaacacat acgagaagcc gtgaatatgc ttcatatatc atggattcta ttacgacagg
    15721 aactccgtca gtgattgcag gaaatgtctt aaataaaggc ttaatcacaa acttaccgac
    15781 agatgtttgt gtggaagtac cttgtttagt cgataaaaat ggcgtgcaac caacctatgt
    15841 cggtgaattg ccaacacaat tagctgcttt aaatcggacg aatatcaatg ttcaagaact
    15901 gacagtagaa gccgctttaa cactagataa atcaaaaatt tatcaagcag cgtatttaga
    15961 tccacatcta tcatctgaat tgtctttaac agatatcaaa aatttagtgg atgatttaat
    16021 cgaagcacat ggggactatt tacctgaata taattaaaaa gaagctgaaa gagtaaggaa
    16081 ttttcaattc cttactcttt tcttagttaa atttagtaaa aatattgaca aaacacttag
    16141 taaacagtta cattagtgtt aacaaaaaga aaacgcattc aaattgtata aggaggagca
    16201 taaaatgaag tggtctaaaa aaattatttt agcttgtata gcactaggag caacgatctc
    16261 tttgactgct tgtggaaaaa atcaggaaga tgcaagcggt aaggtagaaa tagagttttt
    16321 taatcaaaaa aaagaaatga ctcaaacgat tcaggagatt gctaaagatt ttgaagcaaa
    16381 aaaccctgat attcatgtaa aagttgtgga tgtaccgaat gctggtgaag taattaaaac
    16441 acgtatgctt gcaggcgatg taccagatgt cattaatctt tatcctcaaa gtatagaatt
    16501 gaaagaatgg gcgaaagctg gttatttaga ggacttaacc gaagaaccgt atttagagaa
    16561 tatcaaaaat ggatatgcac aacgttttgc tattgagtat cgtgtatata gcataccatt
    16621 aaccgctaat gtctatggtt tttactataa caaaactgct tttgaggaaa tggggattca
    16681 agcaccagaa acttgggcag aatttgaaaa aatagtagct gagattaaag atcaaaacaa
    16741 agtaccgttt gcgattgcag gagcagaagg ctggacttta aatggttatc accaactagc
    16801 tatagcgact gctgctggtg gtgaagagga agcgaacaat gtttggcgtt tctcagaagt
    16861 taatggaatt aatgctgaaa gcaaagaaat gcaaaaagat tttaatcgat tagatttgtt
    16921 aagagaacca aaagctttgc aaaacaactg gcaaggtgcc ggttataacg atacagtagt
    16981 tacttttaca aaaggtgatg cgttgattat gccgaatggt tcatgggcaa tgccgatgat
    17041 tcattcacaa aatccagatt ttgaagtagg aacttttcct tttccagcag atgaagcagg
    17101 tcaaagtcta acgattggcg caggagacct agccttatca gtttctgcaa cttctcaaca
    17161 taaagaagaa gccaaacgtt ttgtagaata tatgacaaca cctgaagcga tgcagaaata
    17221 ttatgatgta gatggtgcac cgtgtgctgt tgagggcgtt atcgagaata ctcaggattc
    17281 accattaagt ggtttaactg aacttgcttt tactgatcgt catttagttt ggttagccaa
    17341 agattggaat agtgaaaatg atttttatac cttaacaaca aattatttac acaatggcaa
    17401 tcaaaaagcc atgattaacg cattaaatgc tttctttaat ccaatgaaag ctgacggaag
    17461 gtaggaaaat aaaatgaaaa taaaagaaat tttaaatcgt tactgggcaa tcatttttgt
    17521 agcaattcca attattttgc agatgatttt tttctacttt ccaatggttc aaggtgcttt
    17581 ttacagttta actaattgga ctggtctgac ttataatttt gattttgtgg gtttgaacaa
    17641 ttataagatt cttttttctg atccgaagtt tatcaaatcg attggcttca ctttaattgt
    17701 gacgctttgc atgattgctg gacaaattgg ttttggtatt ttgattgcgc gggcgttgaa
    17761 cgcaaatatt aaagggaaaa cattctttag agcatggttc ttttttccag cggtcttatc
    17821 gggcgtggtt gtttctttag tatttaaaca aatttttaac tatggtttac caaaaattgg
    17881 tagtatgtta ggcattgaat ttttacaaac cagtctttta ggaacaacat ggggagcgat
    17941 ttttgcagta atttttgttt tactttggca aggaattgcc atgccagtca tcattttctt
    18001 agcgggtttt caaagtattc cacaagaaat tattgaagct gctagaatgg atggggcgaa
    18061 tgataagcaa attttttgga atattgaaat cacctactta ttaccaagta tttcaatggt
    18121 atttattatg gcattaaaag gtggcttaac agcatttgat caaatttttg ctttaactgg
    18181 tggtggacca agcgatgcca caacatcttt ggggttactt gtttataatt atgcttttaa
    18241 aaacaatcag tttggttatg cgaatgctat tgctttagtc ttatttatct taattgggat
    18301 tgtttcactg gcacaattaa aattatcgaa aaaatttgaa atctaggagg tttcccaatg
    18361 aaaacatcaa aagaaaaaaa tatttctaaa tacttgcttt tattattagg gggcttcatg
    18421 attttagttc ccttagtttc aacgctattc ggttcattta aaacaacacc cgatattatg
    18481 aataatttct ttcgttttcc taatccagcg actttggaaa atttccgaag attgttagcg
    18541 gatggaattg gtcattactt ttggaattcg gcaatcatca caatcattgc agtagttgct
    18601 gtgacagcaa ttattcctat ggctgcttat tcactagcaa gaaaaatgac tcgtgggaaa
    18661 gcatacggat tgatgtattt atttttaatt ttaggaattt ttgttccttt ccaagtaatt
    18721 atgattccga ttaccgttat gatgagtaaa attggtttag caaatatgtg gggattaatc
    18781 ttactctatc tcacttatgc cgtaccacag acactatttt tatatgttgg ttttattaaa
    18841 ctaaatgtgc ctatcagtct ggatgaggct gctgaaattg atggggctag tcactttaca
    18901 acttatttca aaattgtttt cccaatgtta aaaccaatgc atgcgacgac tattattctt
    18961 aatgctctat ggttttggaa tgatttcatg ctaccattat taattttaaa tcgtgattct
    19021 aatatgtgga cactaccatt gtttcaatat aattatacgg gacaatattt taacgactat
    19081 ggtccaagtt ttgcttccta tacagtagga attgtcacga ttacactagt ttatcttgct
    19141 ttccaaagaa gtattatagc tggaatgagc aatggagcag tgaaataacc aataaattat
    19201 tctcattata aaggagttca taaacttatg attatatttg acaatcacac acaaacattt
    19261 catttacaaa ataaaaaaat tagctatcta ttaggaatcg aagaaaataa ttatttaacg
    19321 catctttatt ttggaaaaaa gattacgcac tattctggtg ggtatcgcta cccccgaaca
    19381 gatcgttcct tctcacccaa caatgcaaat gcgcaagatc gtttattatc tttagacaca
    19441 ttaccattag aatttccagg atatggacat ggtgatttta gagaaccggc gcataatatt
    19501 aaattaggaa acggtagtcg tgtaaatgat tttcgttatg atagctacga aattcaaaaa
    19561 ggaaagccca gcttagcggg attgccagcc ttatatacgg aagatgatga tgaagctgaa
    19621 acgctgatta ttcaattgaa agacgcgatt agtggattga ctttacagtt gttctacaca
    19681 atttatcaaa atcacagtgt aattattcgt tctacgcaat tagtaaataa tagtgatgaa
    19741 acagtggaga ttaaccgctt agctagtcaa tcaattgatt ttccaaatcg tccactcgag
    19801 ttaattcatt taaatggtac ctgggcaaga gaacgtcaaa tgactagaga aaaaattcat
    19861 acagggataa aagtattaga tagtaaacgc ggatcaagca gccatcaaca aaatccgttt
    19921 gttgctttag tagatgccaa aacagatgaa tttcaaggag aggcatatgg atttaattta
    19981 gtttatagcg gtaatcacga aacggtgatt caaaaagatc catttgacca aacacgggtc
    20041 attatgggaa ttaattcatt taattttggt tggcaactaa aaactggcga ctcatttcaa
    20101 acgccagaag tcgtgatggt ttatagtaat gaaggtctga atacgttatc acagacgtat
    20161 catgaattgt ataatcaccg tttaattcgt ggaaactatc gtttaaaaga acgtcccacg
    20221 ttaatcaaca actgggaagc aacgtatttt gattttaacg aagaaaaaat catgaatatt
    20281 gtcgatgatg cgaagaactt gggcattgaa atgtttgttt tagatgatgg ttggtttggc
    20341 ggtcgtgaaa atgacttcac ttcattaggt gattgggttg aaacagaagg caaacttgct
    20401 tgtggtttgg aaaatttagc caagcaagtg catgataacg ggatgaaatt tgggatttgg
    20461 tttgaaccgg aaatgatttc tgaagatagt gaactatttc gagcacatcc agattgggca
    20521 ttgggtattc caggtcgtga ccgctcaatt agtcgaaatc aatatgttct tgatttttca
    20581 agacaagatg ttcgtgataa tatctatcaa caaatgacgg cgatttttga tcgtgtaccg
    20641 attgattatg tgaagtggga tatgaatcga aatatgacag aagtctattc tgtcttgttg
    20701 gactcaacga tgcaaggtga agtggctcat cgttatatgt tagggttata tgaattttta
    20761 gaaaagttaa caacgaatta tccaactatt ttatttgaaa gttgttcggg tggaggcggt
    20821 cgttttgatg caggtttctt gtattacatg ccacaaactt ggacgagcga caataccgat
    20881 gccgtggctc gtctgaaaat tcaatatgga accagcttag tgtatccaat ttcaagtatg
    20941 ggtgcgcatg tttcagcagt cccaaatcat caaacaggaa gagaaacgag tttaactatt
    21001 cgtggcaatg ctgcgatggc tggtgttttt ggttatgagc tagatctaac gcaattaact
    21061 tctgaagaaa aagcagaaat taaagaacaa gttgcctttt ataaaaagca tcgccaactt
    21121 ttgcaatatg gaacttttta tcgcttggaa agtccgtttg aaggcaatga tactgcatgg
    21181 atgtttgtct caaaagacca aacagaagcc atggtctttt atttccgagt attagcagaa
    21241 gctgcgcatc cattagtcac attgaaatta gcaggattag cagaagaagc aacttatcaa
    21301 atggaagatg taacatttgc tggcgatgaa ttgatgaatc tcggttttta tattgactca
    21361 gaactacatg gtgattatgc gacacaacgc ttttatttga aacagatata gtagcttgaa
    21421 tttacaatat aagtgcaaca ccaaaagaga ctcattccaa acgaagaaaa tatgttttat
    21481 ttacacgcat ttactaaaaa gcagcctgat ttgaattggg aaaatgaaga agtacgtgaa
    21541 gaaatctatg caatgattaa ctattggtta gataaaggac taggcggatt tagaatcgat
    21601 gcgatattaa acatcaaaaa aaaactagag tatggcatct ttgaatctga tggagaagat
    21661 gggttagcct tcattgggcc ctggattctt aatcagtcgg gcattgaagt gtggttaaaa
    21721 gaaatgagag agcggacatt taaaccacat aatagtatga cggtagcaga agccgatgtt
    21781 cctaatgaac gtttagatga atatattggt gaagatggct attattcgat ggtttttgat
    21841 tttagttata cggatattga tgtacctgaa actggtgaat ggtttaaaga tagccaatgg
    21901 acatggacgg atatgcgtac caatattttt acgaatcaat tagttacgca agataaaggt
    21961 tggggcgcct tatatttaga aaaccacgat caaccacgtt caattaataa atatattcca
    22021 gaagaatgga tcaatgatta tagtaaaaaa atgttggcga cgctttttat gttactaaga
    22081 ggtacccctt ttatttatca aggacaagaa ctaggaatga ccaacataga aatggcatca
    22141 ttagaagatt ttgatgatgt agctacgcat tctcagtata aacgggcatt agaatatgga
    22201 ttggcaccag agcaagcatt gaaagctgtc aataaacgaa gtcgggataa ttctcgcaca
    22261 cctatgcaat ggaatacgca aaaaaatgcc agattctcaa cttctgataa tgtatggtta
    22321 aaagtaaatc ctaattatcc agaactcaat gcagaagctc agttaaccaa tcaacagtcg
    22381 gtattcaatt attatcatca attaattaaa ttaagaaaaa atagtgtata caaagacgtg
    22441 ctaatctacg gacaatttct accagtgaaa gataaaaatg aacatatttt gctttatgaa
    22501 cgccaattag aagataaaat cgtattagtt atcttaaacg ttacagcaac agaacaagaa
    22561 tattatgtag atgaacagta ccaacaagta ttaatcaata attatgaaga tgttttatta
    22621 gatcaaagtc gaaaattgcg tttacgaccg tttgaaagta ttgtattagc taattatgga
    22681 ggacaattat gaaaattaaa aacgaagcaa tgctcatcac ctatgcagat agtttaggaa
    22741 aaaacatggg cgaattaaaa caagtcttag acaaacattt acaaggtgtt attggtggtg
    22801 tacatctgtt gccttttttt ccatcgacag gtgatcgagg ctttgcgccg agtgattata
    22861 cgacagttga tccagctctt ggaacatggg aagatgtgga agcattaggt gaagattatt
    22921 atttgatgtt tgattttatg attaatcaca tttctcgtga atcaaaattt ttccaagatt
    22981 tcaaaaaaaa ccatgagcag tcaccataca aagaaatgtt tatccgtatt catgaatttt
    23041 tcccagaaaa tcgaccaaca caagaagata ttgatttaat ctacaagcgt aaagacaaag
    23101 cgccgtttca aacggtagaa tttgcggatg gtacgacaga agaggtttgg aatacctttg
    23161 gggaagaaca aattgattta gatgtgacca aagaagttgt gaaagaattt attcgagaaa
    23221 ccatcaaaga tatggcgaac catggctgtt ctttaattcg attagatgcg tttgcttatg
    23281 cgattaaaaa attagatacc aatgatttct tcgtggaacc agatatttgg gaacttttag
    23341 acgaagtgcg tcaagaagca gcgaaatatg acgtcgaatt attaccagaa atccatgagc
    23401 attattccat tcaaatgaaa attgccaatc atgattatta tatctatgac ttcgccttgc
    23461 caatggtcac tctgtattca ctttatagtg gaaaatcaga acgtttagcc aattggttga
    23521 aaatgagtcc gatgaagcag tttacaacat tggatacgca tgatggaatt ggtgttgtgg
    23581 acgctcgtga tttattaaca gatgaagaat tggattacac ctctgaggaa ttgtataaag
    23641 tcggggcgaa tgtgaagaag gtctattcaa gtgccaatta caataattta gatatttatc
    23701 aaattaacag cacctattat agtgcattag gcgataatga ccgtagctat ctgttagcac
    23761 gtgcgattca agtatttgcg ccagggattc cacaaattta ttacgttggg ttattagcag
    23821 gaaaaaatga tattgaactg ttggaagcaa cgaaagaagg gcgtaatatt aatcgtcatt
    23881 actatgattt agccgaaatt gaagaagaag tgcaacggcc agtgattcaa caattatttg
    23941 cacttttaaa atttagaaat gaatctgcgg cgtttgattt ggatggaacg attgaagtag
    24001 caacgccatc agaatcagaa atagtgatta cacgtaaaaa taaaaacggt agtgatagtg
    24061 caacattaac ggctaacttg gatacgaaag aatttatcat tgagtctaaa gatataaaaa
    24121 ttttcactag tgttgcatta aaattttaac ctattccata gaacaaaaaa tcctttatac
    24181 tagcacttgg atgacttatc caagaccaat aaaaaggact catacatgga taagtataaa
    24241 acaaatacag catttaataa atggttttct gccataaaat taggaaaact ttctgtcaac
    24301 tcccaaaaat gtatttttga ttacaattcg tatcgtaaaa aactgtcttt tgaagctgtg
    24361 ctcaagctct ttctctacgc agttaatgaa gaaaaagaaa gtttgcgtga tttgagtaca
    24421 gctcttatcg accgatcgct ccaactagaa accgatgtgt catcgattag tcatacccaa
    24481 ttgtctcgag catttaatgc acttgaacct aaagtgttag aagaaatttt tcaacaactg
    24541 ttagaaaaag ttcaacatca aatcaaacca accaagcgaa atcatctgta tctgattgat
    24601 tccagtacgt tttcgcttag tttaaaacgt cataagtggg cgactttccg tcaaacaaaa
    24661 tcaggtgtca agttacactt aaactattgt tatatggacg atactacgat gtatccaact
    24721 gattttacct taacaaatgc ccatgaacat gatctcaatc aactaccagt attggtgaat
    24781 caaccagagg caacttacgt gtttgatcgt gggtattagg actttgaaaa gatggatcag
    24841 atgcattggg atggctattt cttcgtcact cggattaaaa aaaacaccaa agttcacgtg
    24901 atagatactc tggaaacatc gccagaaaca gaaattctac gagacgagct ggttcgttta
    24961 ggatctaaaa cctatctaac cgccaatttc cgactagtca ctgttcaaga taagaacgga
    25021 cgtgtgtttc aatttatcac caatcgaatg gacgtctctt caaaagaaat ctctgatatg
    25081 tatcacgctc gttggcaaat cgaattgttt ttcaagcaca tcaaacaaca catgaccatt
    25141 aaaaccttct tttctcaaag tgaaaaaggc gtgcagaatc aattgatttt aacgatgatt
    25201 tccgctctcc tcacgtttct catcaaattg gagacgaaaa cggaaaaatc agtgttccaa
    25261 atcaaacgat tctttcgtta tttactgttt cagccctttg aatgttgtct agaaaaatta
    25321 attcccacat gagaaaaaag aaatttggct cgttactttt ggttttcagt tgaataacag
    25381 ataaaaaatt ttctggaaaa agtcatcctc tgcgtcagca cagttttttt tgacttattt
    25441 tcagaggaaa tagttttcag aatattctaa aatgttttat gcaacactag tgatattttt
    25501 tagaattaga tttctaatgt gcattttttg agtattaagg agcaagtata caaagattta
    25561 taaagctgat taaatttgaa taaaaatatg caggaacgct actattttta gtagtgttct
    25621 tttttatatt tgaataaaaa atgtggtggt taaatggtgg ttataacaaa aaatgaatta
    25681 atgaaattag gttatgggaa gtatcagtct gagagaatca tacgacaggc aaaagcaatt
    25741 atggtttcaa aaagctatac ctattacgaa aatagaagat taggactagt acctgttgaa
    25801 tctgtagaag aagttttagg tatgaagata gaggtagagg aggattttca gaatcatggt
    25861 taattttaaa aaagataaaa atgggaaata tcattttact gctagtttgg gattcgacaa
    25921 acgaacaggg aaaagagtac aaaaaaacgt agaggggtta aaacagttaa agaagaaaaa
    25981 aagcgtatac cgaacttgtt tctaattatg gaaaagaagc agaaacttct caaagtacta
    26041 tgttgtttga ggagtttttt tataaattat tcctacctta ttatcaaggg aaagtagaag
    26101 aaagaacata taaaaatcgg ttatctacaa tcaaaaaaac ttttcttatt tctttaaaat
    26161 ggagttaaga gaaatacgtc caattgacat tcaaaagtgg caaaataatt tattgagcaa
    26221 atataaaaat tcttatgtaa gaaacatgta tgggattttt cagatgtcat tagatcgagc
    26281 agttgttttg ggactacttg aaaaaaatcc tgcaaaagtt attggaaatg tgaagaaaag
    26341 gaaaactaca gttgattttt ggacaaaaga agaatttaca aaagttattt caacttttta
    26401 tattgatgat ttgtaccaac attttggttt tatttgtatg tggttacttt ttatgaccgt
    26461 tatgcgtttg ggagaagcca ctgcattaac ttggaaagat attgattttg aaaataaaaa
    26521 cttgcgtgtg aataaaacac tctattataa gagagccaaa acctatgagt ttgtagaacc
    26581 cgaaacggct tctagtattc gtacaatcga actagacgac aatacaatta cctttttaaa
    26641 agcttggaag gagcgtcaag tttcaatagg aggcatgtcg tttgtacttt cttataatgg
    26701 aacacctact cagaaatata caatccgtca tattatgaga agacatgcaa cgatcgttgg
    26761 tgttcatata atccgcatac acggattaag gcatagtcat gcttctttgc ttatatcaca
    26821 ataaaaagat gcacttcgaa ttaaagaaag gttaaaacat gctgatgtac aaacgacttt
    26881 aggaacttat ggtcatttgt atctaaattc attgagagag ataacaaatg atcccacaga
    26941 ttatatccaa actaattttt ctaaaatcaa accgacacct atttacgata ataaaaaata
    27001 aatgataaac aaaagtagga tcacaccaaa agaaacacac tagaaaaact ataatacagg
    27061 cttttctagc gtgttgtttc ttatttccgc ctatttatac attatactca taaagcattt
    27121 aatgcatcga tcgtagccgc attgtctata tgggtatata aagttgttgt attggtactt
    27181 gaatgtccca attgttgggc agttaatacc aatgaatttg tctgttggta gagtctagta
    27241 gctaatgtat gacgcagttt gtgagggctg acttgaattc taaagccttt tgaatatttt
    27301 gctaccatcc gttcgattgc aataccggat atacgcttgg cttctccttg gtaaattgct
    27361 agaaataaag ctttttcgtt ttctgttact ttatatcgtt ctttccgaat agctagataa
    27421 ttagctaaat attccattgc gaatgctgct atattgacag catctctttt atttcctttt
    27481 ctagttacta ccacattatt cttatctaaa gataggtcat ctaaattaat atttataacc
    27541 tcggagagtc tcaatccaga tcctaaaatc agtgcattga tcgccaggtc tctttctaca
    27601 tctcgttgat aatagatcaa agctcgttta ctcaaagtat ttttatattc attggcaata
    27661 taatctagat aattttgtgt atccttatct agaaataatt ttttctcaat ggcattggca
    27721 cgtgcagcta acgtagtttt atctgtgatg gttgctactt gatccatagg attattgtct
    27781 aagtatggtt ttcctagagg attattggaa cttttcgaaa gatatttaaa tagaaccttt
    27841 aatgccgtga tatttctatt gatagtagaa gtagatagac ctaagttatt attatctttg
    27901 agcttagttt ttgttgtctc tttttttaca ttttcttttg tgcgacgttt caaatgagat
    27961 ttgtacaagt ttaggtcata ggcagttaac tgtcttatcg aggtgataga agtatcagga
    28021 acgatagact cactcagaag ccattcataa aagagcttat aattttttag atattctaaa
    28081 gtggttcgag gtgattgatc tttatcctca cgatcttgaa caaaatcttt tatataatcg
    28141 ggcatatgaa gtatgattgt atctattttt tgatatattt ctgtattttt catgtcattc
    28201 atcctttcaa taagtagggg agaagagtat ctctataatt ggactatacc aattatagaa
    28261 tgttttgtca attggtatgc tctaaaattt attaatttgt ttcaaaaaaa gcatataaac
    28321 aggtatacgt ttaataattt aaaataaatg gtatacataa atagggaagg aaatagaagg
    28381 gagcaatcta aattttttga attttcttaa ttaattatat agaaagaaca atctttctat
    28441 agagttagac atgagaggga ctatgcttga tttaaaatca acaagcgtat atactgtata
    28501 tacaaagaaa aaggagggag aagactatgg caattaaaga aagaaaattt agggaagtgg
    28561 ggaactcagt agtattaact atttcaaagg agtttcttaa agaaaatggg ttaaatcccg
    28621 gagatacggt ttttttagat gaagaaaaac ttcgagcggc aattacaaaa aaacaagcct
    28681 tatctgatga aattgattta gcaatatctc aagcattaac tgaatatgat actactttta
    28741 gagagcttgt cgataaatga gtattcatta cctagaagcc aaagatatta taaaaatgaa
    28801 tgtaatccaa ataaaaaaat actctcctaa tgaacccctt ggtgtaaagg atgcgaatgc
    28861 tttagaaatg tgtgtagggc aacttcgagc ctcagctttt ggtgaagagg tttatccttc
    28921 gatttatgaa aaagcagcta tcctacttat acaattgata aagaaacatc cttttcacaa
    28981 tgcaaataaa cgtactgctt tcttagctac ttttgtcttt ttgaaaatca atggacagct
    29041 tttagctatt gatcaaaaag atgctgtaaa tttagcggta tatattgcta cgtatgataa
    29101 ggatttcgat caactaaaat atgaagtgat caacactata aagacacata cgcagcccat
    29161 ttaaggagtt actgtatgtg tctttatagg gttattagct gctatagcga aataattgaa
    29221 gtaaattaca aatagctata attcaaatcc aactatatag aaaacgtaaa ttttctatat
    29281 agttggatta agataacgac tatttatttt atcatatttt tactataccc tatactttta
    29341 cttggtatat ttatgaataa tcgcaaattt acccctatac tactagtttg taagttagat
    29401 taataaaaat actaaagtta tttgttcttt tattaaatac acctacaact tataaattga
    29461 tggtatttag ataacacgaa agaataaaaa taacagctga tagtaaagtt tgttgacgat
    29521 tgctactcaa tcgaaagtga gttagtgcgt atgaacttgc ttctgaaaac ttatgaaatg
    29581 aaagaccttt tacatcttgc agaaatcggt actgggatta aactaagcaa taaaaactaa
    29641 tgttcccctc attctttgaa cttctactat ttgatttatt tattctgata aatgacgatt
    29701 tcaccttcag gatcttcaaa tcccgtatag aatttattta agatctcttc agtatcagta
    29761 ataggatagg cgataggcca catacccttt gatggttaga ataaactgct caaaatctgt
    29821 atcgtatgtt ggctttagca tattaacaaa ggctatctcc aaattataaa ccataagcgt
    29881 tgaatcgttc ttaacaattt caattcttcc taacccaaag tcagaagata taattaccgg
    29941 acttgcctta cccttcatgt gggttgttga gattccatat ttaaagggaa ttgtcggtac
    30001 tatcggcggt ctgaaaaaca tcccaaaaat aaaaaaatct ttcccctgct agtctcctaa
    30061 gtccctttgt agcggatatg gcaccataat ctcattttat cgaagtgagt catggtttat
    30121 ctgttggcca accaaaattg atacaatcta acgatgcacc ctctacggca ttctagggga
    30181 gtgcacttgc attcacccaa taaaaaccgg cagttagcaa agaacttatg tagtcgtatg
    30241 ttcttactgt taacatacaa gaggtctcct ttccacaatt gcttagcagt aaacgggaat
    30301 ttacaaagct aatctcttcc attttcttat ctttgttcta aaagtcccaa atggagcaac
    30361 tgtatttaca tgaataaatt tatatacttc ccatacagcc gttttggttg cctcgtctgc
    30421 ccattttctt ttgtgtgcag tgaatagatc ttcatcactc attgaatcaa tcatcatgtt
    30481 aatatcatca atattacgat tgagaatttc ctctaattct ttcaatggta gatgtgcata
    30541 ggtatttgta aaccacttat ataactctcc taactgattc catttaaatt gatcagaagg
    30601 cgttttaaca gataatccat ttctttcatc attttcccat tttagaatta aattcgtcca
    30661 accaacttga tatgctaaat tttcagcagg tgttctatcc acttcctcaa ctcttttatc
    30721 cttcaattct tcaggaatag tttcaaattc taaaatatat ttagtataag tcctatttat
    30781 ttcttttttt aattcttctt tatttttata tgttctcatt atattacctc cataaattcc
    30841 taattgtctc ctttaattca tctataattt catccacgat attttaatcc gttctacaat
    30901 gcatatctga tatgcattga ctgtctaact catatgtgaa tgatttataa aatatcccaa
    30961 cgtctcatct aaagaaatgt tgatcgtcag caaggagcta aaaaatacga atatatttct
    31021 tttgtagtaa aatcggactg gtttgcatat cattttcttc gtaaatatca aaatcatgtt
    31081 aactactatc aattactaat aaaaactacc actttaagcg ttaagaactg ttaagtttag
    31141 ttaagtgagg atagttcaca aaatccattg ttaaacttca cgaaagtggt aaaactcaaa
    31201 atgctttata taggattaca gcatcgctct ttcttcgatt gcgcgttggg taaaaaatat
    31261 tatgaagtaa aaattgacga tgacacgttc cttacggctc atcagatgaa acagctacaa
    31321 aaacgaaatg atcaactcga agaaaattta attctaaaaa agcgattgcc atattcacgc
    31381 ctcgctcgac gaactattaa aggtcaagat ttacctgaag atcatccttt ttaggaaatt
    31441 gaagcttttt atgttgcaca atgcatttat aatactacaa tgatgttttc accagatatt
    31501 cttctagggt gcggtgtaat gaaacagata cacttgcaaa agaaaattca ccgcgaattt
    31561 actaaaatta tgaataatta tgttaatctt ccgaaatttt tttccaaaat taagggatca
    31621 agctggtgtt atagattgtt taaggttggc aaaaaaataa cttcttttta aacaattata
    31681 gaaaaccgat actctgttaa gttggttgac acggtgtcga tttttatgac tttcttattc
    31741 tgaaacttca ctaacggaca gtacctaaaa agtctatagt atggacgttt acgagtacta
    31801 ttttatcaaa aatgctgctc atttcaactt atatgtttta gtgaactacc cccacttagc
    31861 ttggataggc tacaattaac tagacagaaa aattaaggtg tgtagactag aagaaaacat
    31921 accaggagga atttttatgt ctaagagaac acgaagaact ttttcacaag aattcaagca
    31981 acaaatcgtc aatctttact tagctggaaa gccacgtgta gaaatcattc gagaatatga
    32041 actaac
//
LOCUS       DAJSBL010000028        31560 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.028, whole genome shotgun sequence.
ACCESSION   DAJSBL010000028 DAJSBL010000000
VERSION     DAJSBL010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31560
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.028"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            49..510
                     /gene="mscL"
                     /locus_tag="ONT85_001564"
     CDS             49..510
                     /gene="mscL"
                     /locus_tag="ONT85_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HCR2378542.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(570..1016)
                     /locus_tag="ONT85_001565"
     CDS             complement(570..1016)
                     /locus_tag="ONT85_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR2378543.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGE
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(1109..1354)
                     /locus_tag="ONT85_001566"
     CDS             complement(1109..1354)
                     /locus_tag="ONT85_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HCR2378544.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(1470..2048)
                     /locus_tag="ONT85_001567"
     CDS             complement(1470..2048)
                     /locus_tag="ONT85_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HCR2378545.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSVISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(2125..2655)
                     /locus_tag="ONT85_001568"
     CDS             complement(2125..2655)
                     /locus_tag="ONT85_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HCR2378546.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(2659..3519)
                     /gene="rlmB"
                     /locus_tag="ONT85_001569"
     CDS             complement(2659..3519)
                     /gene="rlmB"
                     /locus_tag="ONT85_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HCR2378547.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(3512..3907)
                     /locus_tag="ONT85_001570"
     CDS             complement(3512..3907)
                     /locus_tag="ONT85_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HCR2378548.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(3904..5316)
                     /locus_tag="ONT85_001571"
     CDS             complement(3904..5316)
                     /locus_tag="ONT85_001571"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HCR2378549.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(5313..5849)
                     /gene="cysE"
                     /locus_tag="ONT85_001572"
     CDS             complement(5313..5849)
                     /gene="cysE"
                     /locus_tag="ONT85_001572"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HCR2378550.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     misc_binding    complement(5935..6174)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(6171..7631)
                     /locus_tag="ONT85_001573"
     CDS             complement(6171..7631)
                     /locus_tag="ONT85_001573"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HCR2378551.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(7801..8958)
                     /locus_tag="ONT85_001574"
     CDS             complement(7801..8958)
                     /locus_tag="ONT85_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HCR2378552.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(8992..10362)
                     /gene="radA"
                     /locus_tag="ONT85_001575"
     CDS             complement(8992..10362)
                     /gene="radA"
                     /locus_tag="ONT85_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HCR2378553.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKTAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(10425..10952)
                     /locus_tag="ONT85_001576"
     CDS             complement(10425..10952)
                     /locus_tag="ONT85_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HCR2378554.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            11182..11988
                     /gene="proB"
                     /locus_tag="ONT85_001577"
     CDS             11182..11988
                     /gene="proB"
                     /locus_tag="ONT85_001577"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HCR2378555.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            11985..13232
                     /locus_tag="ONT85_001578"
     CDS             11985..13232
                     /locus_tag="ONT85_001578"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HCR2378556.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVIGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            13308..13652
                     /locus_tag="ONT85_001579"
     CDS             13308..13652
                     /locus_tag="ONT85_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2378557.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(13769..14095)
                     /locus_tag="ONT85_001580"
     CDS             complement(13769..14095)
                     /locus_tag="ONT85_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2378558.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(14710..15912)
                     /locus_tag="ONT85_001581"
     CDS             complement(14710..15912)
                     /locus_tag="ONT85_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR2378559.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            16029..16544
                     /locus_tag="ONT85_001582"
     CDS             16029..16544
                     /locus_tag="ONT85_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HCR2378560.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(16743..17189)
                     /locus_tag="ONT85_001583"
     CDS             complement(16743..17189)
                     /locus_tag="ONT85_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HCR2378561.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(17236..18714)
                     /gene="msr(C)"
                     /locus_tag="ONT85_001584"
     CDS             complement(17236..18714)
                     /gene="msr(C)"
                     /locus_tag="ONT85_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HCR2378562.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(19068..19697)
                     /locus_tag="ONT85_001585"
     CDS             complement(19068..19697)
                     /locus_tag="ONT85_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HCR2378563.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            complement(19681..20145)
                     /locus_tag="ONT85_001586"
     CDS             complement(19681..20145)
                     /locus_tag="ONT85_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCR2378564.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(20632..21900)
                     /locus_tag="ONT85_001587"
     CDS             complement(20632..21900)
                     /locus_tag="ONT85_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HCR2378565.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(22131..22868)
                     /locus_tag="ONT85_001588"
     CDS             complement(22131..22868)
                     /locus_tag="ONT85_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR2378566.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(22935..23504)
                     /locus_tag="ONT85_001589"
     CDS             complement(22935..23504)
                     /locus_tag="ONT85_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HCR2378567.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            23632..24504
                     /locus_tag="ONT85_001590"
     CDS             23632..24504
                     /locus_tag="ONT85_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR2378568.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            24846..25718
                     /locus_tag="ONT85_001591"
     CDS             24846..25718
                     /locus_tag="ONT85_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCR2378569.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            25715..27673
                     /locus_tag="ONT85_001592"
     CDS             25715..27673
                     /locus_tag="ONT85_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCR2378570.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(28005..28856)
                     /locus_tag="ONT85_001593"
     CDS             complement(28005..28856)
                     /locus_tag="ONT85_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR2378571.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKKLEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            28979..29446
                     /locus_tag="ONT85_001594"
     CDS             28979..29446
                     /locus_tag="ONT85_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HCR2378572.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(29709..30191)
                     /locus_tag="ONT85_001595"
     CDS             complement(29709..30191)
                     /locus_tag="ONT85_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HCR2378573.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVIA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(30291..31154)
                     /locus_tag="ONT85_001596"
     CDS             complement(30291..31154)
                     /locus_tag="ONT85_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCR2378574.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGARLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYELVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
BASE COUNT         9033 a         6670 c         5709 g        10148 t
ORIGIN      
        1 tttatttttc gatatactaa agacaaataa aagaagggag ggtcattcat gatcaaagaa
       61 ttcaaagagt tcatcatgcg tggagatgtc ttggatcttg ctgtcggcgt tgttattggt
      121 agtgcgttta caagcatcgt aaaccaagtg gtgcaaggct tgatcacacc actaataggt
      181 tggtttgtcg caatgatcac aggtacttct gatttagaag gagcactttc aattcttgac
      241 tggtcgccga caaaaggcgt cacatttgcg tttggtaacg ttattagtgc tatcattaca
      301 tttctgatca ctggttttgt cttattctta gttgtcaaag cggcaaaccg tgcgaagaat
      361 ttacgctcca aagaagagca agtggaagtg gaagaaactc ctactgcaga agattatctt
      421 agtgacatac gcgatcttct tgccaaacaa gtggcagaag acaatacaca tccagtagag
      481 caaacaaatc tttccgatac ttctaaataa taaagaatct aaaaaaaaag aaagccgatt
      541 ttcagtgtcg gctttctttt tttagattct taacttgcta cttgttcctt tttcatgtcg
      601 atatgtggaa tatcatcttc caaatacaca tctgagattg ccttgaatcc aaacgactca
      661 taaaaagctt gtaggtattc ttgtgcgccg atcacgattg gtttttctgg aaaatttttc
      721 tggatcatgt taatcgtttc ttgaacaatc tttcttccta aaccatcttt acggttgtcc
      781 tgatgaacaa ggacacgtcc aaaatggata ctctccgctt cttcatatac tcgcgtgtag
      841 gcaaggatct tcccatcagt atcttgaaaa aacgtatgcc aagcttctcc atcaatgtca
      901 tcaacttctt gataaggaca ctgttgttcc acaacaaaaa ctgcgattcg ttctttgacg
      961 atcgcgtaaa actctgctgt tgacaattct tcaaatttct ttacctgata ttccactaag
     1021 caaacgcccc ctgctttttt attcttagca taccgattcg cacacaaaaa agcaaacgaa
     1081 ccttacgaat ctcaggctcg tttgcttttt aaactgcttc agttaaaaat tcgatctcaa
     1141 ctgtccgagt aagtacatct gaatagctgt aagatactcg ttcaaaagaa ttttcttcag
     1201 gatccaaatc aacaacgaat actgaaggat aggtttctgt taaaacgccg cggcgttctg
     1261 tttgacgctt tcttcctgtc tgagcaacca acatgattgg gctgccgata cgaccttcta
     1321 aatctttctt gattgaagct aaagttgttg gcatacagtt cacctctact atcaactata
     1381 ccacatattt ttaaaatttt caagtttacc ataaaaaaaa aaagaaatgc aataaaaata
     1441 aataagaagc tcattaagat ttttctaaat tattcctcat tataaaattt caagtgtttt
     1501 tttattttga gttttactct cccgtaagcg ctcattactt gcttgtgatc cattgctaaa
     1561 cattcagcca tgtctacaaa atcaacgcct tgcatataat aatgaaaaat tttagcttct
     1621 atttttgaaa actggatttc gcaattttcg attgcttctt ttaataaaag atgctgaatt
     1681 gctaaaggtt cttgcaatgt gatctctgct aaaaagtctg gtccttctgt ctctattttt
     1741 tggtcgattg agacttcatc tttttgatca cgacgcttca gcgcgttctg cttcctcaac
     1801 agagaataga tttgattgct tataacgctt ttataataac ttccaaagct aacacctttg
     1861 cttttttcgt aagttctcaa tgcctgatat aaagcgatcc ttgcttcttg ttcccaatcc
     1921 tcagaaacat aatttcttaa ataataggtg tttttcaaac gtagtaaaag tggaaaatat
     1981 ttttgataca gggcagcaaa tgcttcttcg tcaccattac gtgcagcgcg caataactcc
     2041 tcagacataa aataattcct ccataaattc gtttatggag gaatcttatc atcaatgatt
     2101 catcgaactg attagaacga tatgttattt attttttctc attaaatcgt ccaatttttg
     2161 ggacaatttt tttaactgct cttcattcca tggagaatta cgtcggaaat caataaaatg
     2221 catatcttta ttttcttggg ctattgtttt ttccgttttt tttatcgtct tatataattc
     2281 ataagcagaa gttcgtaatg ctccttggga aaagatcatc cactgttctg ctaaatcgct
     2341 tgtcgccact gtcacttgtg tcaaacggtc attcagctga ccagcgattc tctcgatata
     2401 actgtcagcc gtttcgtctt ctttcgtaaa aacaacatcc aactggtatt tcttataatt
     2461 ttgttggatg cccggaacta gttgggcatc gaagacaacg atgacttcca ttccttcata
     2521 tttcgcataa tttgataaac gatgtaaaag agcttctctt gcatccgcca ttttgtcctg
     2581 ctttttaagc tggacaagtt ccggccacga accaatcatg ttgtagccgt ctacaatcaa
     2641 cagctgtttt ttcatggtct actccccctg acgttgggaa aaagcctgat acataagcaa
     2701 accagcagca acactggcat ttaagctctg gacgtgcccg gtcatcggga tcgtcagcaa
     2761 ttcatccatt tcttttttca atcctggact cattcctttg ccttcattac caatgactaa
     2821 agcaattgat cctttggcat tccatttttg gaagtttgtg ccggtcatat ctgtgccaaa
     2881 tacccaaaat cctgtttctt tgagctgttg cacagcttgt accaaattcg tcacgcgtgc
     2941 aacagggaca tgttcgactg ccccagtgga agtctttgtt acgataggtg tgattccaac
     3001 tgctcggtgt tttggtatga tcacaccatc gacgccactt gcgtctgctg tccgtaaaat
     3061 cgacccaaaa ttgtgtggat cttcgatgct atccaaaatc aaaaagaagg ggtcttcttt
     3121 tttctctttt gttcgttcaa tcagttggtc aagggataaa tattcataag gtgtaatcgc
     3181 aagaacgact ccttgatgca cgccgtgatc gctcaaagtt tctaattttt gtttaggaac
     3241 ccatttgact ggcacagctt gttcacgtgc gatttctttc agttcttcta ctttttctcc
     3301 tcgtgaatct tcttgaagaa agagtttatt ccctcgccct tgttgtagcg cttctactgc
     3361 ggcatggtgc ccaaagacga aattatcttc taatgcttct tcattttgat cagaaactga
     3421 ctgatttcgt ccagtttgct ttttatttga atatctattt tgttttttgt cttcaaatcc
     3481 acgtttcgat tgatgtttgt ttgttctttc tttacccatt tttttcacca actttctgaa
     3541 tacaccagcg gatcaattct tccatccgct cggtttgttt tgtcaaatgc agatagccca
     3601 tgagcgcttc gaaccctgtt gcgacccgat acgtcgtaac atcagcattt tttgcgctcg
     3661 tatggctttt agcatttctt cctctgcgat aataaagttc ttcttcttct gtcaaaagat
     3721 gttcttccag catttgctga atcaaaaagg cttgtgcatt cgcagaaaca taattcgttg
     3781 ccgcacgatg cagcttattg ggttttgttt ctccttgatc aatcaggtgg tcacgaacat
     3841 agatttcgta aatcgcatct ccaacataag ccaaggctaa accgttcaat tgtgtgtaat
     3901 ctctcatgct tctcttctcc atcgggttcc ttgcggtgta tcttctaata agatcccttt
     3961 ttctttcaac aaatcacgaa tttcgtcgct tcggacaaaa tcacgattgc ttcttgcttg
     4021 atttcgttct tcgatcaatt gttcaatctc ttcatctaat aactcttctg ctgtaaataa
     4081 aataccgaag atcgaaagcc attgcgagaa taaagcttct ccttgtgcca agacagcggc
     4141 agaaactttc ggctggtcta gataagtgtt cagccactta gcaagctcat agaccactgt
     4201 gatcccattt gctgcattaa aatcatcgtc catttcttcg atgaatcgct gttctaaatt
     4261 ttctaacaaa gcgattttat ccgcatcatc tgccaaattc tctgaggcag tttcttggcg
     4321 gaatttcaaa ttgtcaaacg tattcttcag tcgttgtaaa ttggtggctg cttctttcat
     4381 cgtcgtttca ctgtagcgga tcggacgacg gtattgtgtg gtcgccatga agaaacgcaa
     4441 gacttgtgga tcaacgtttt tgatcatttc atgtacagtg atgaaattcc ctagagattt
     4501 actcattttt tcatcgtctt cgccaatcgt tacatagcca ttgtgcatcc agtaattagc
     4561 gaattttttc cctgttttcg cttcactttg tgcgatttca ttttcatgat gaggaaattc
     4621 taaatcttgc ccgccaccat ggatatcgat cgtttctcct aaatgttttg ttgccataac
     4681 ggaacattca atatgccaac cgggacgtcc tttgccccat ggtgaatccc aagaaatctc
     4741 gccaggtttt gcttgtttcc ataaagcgaa atctaatggg tcttcttttt gctgctgttc
     4801 ttcccctgta cgctgacttg ctccgacttc cagttcatcg atggattggt cacttaattt
     4861 cccataattt tcaaatttac gggtacggta atacacatca cctgaagatt cataagcata
     4921 gcctttttca accaacactt gaataaatgc taaaatatct ggcatatgat ccatgactct
     4981 tggatgcagt gtagcaggtt cgacattcaa cgctttcgta tcttcttcaa atgctcggat
     5041 gaaacgatca gcaacttctg gtgctgtaat acctaattcg tttgctgtac ggatgatttt
     5101 atcgtctaca tccgtgaaat tcgatacgaa atcgacttta tagcctcgat attcaaaata
     5161 gcgtcgaatt gtatcgaaag caatggtgct acgagcattt ccgatatgga tataattata
     5221 cacagtcggt ccgcagacat acataccgac atgtcccttt tgtataggga tgaattcttc
     5281 tttttttctg gtcaatgtat tgtagatttt tatcattctg atctcctcac tttttctcct
     5341 ttgattcgaa cgacttttgc tggtactcct accgctgtag cgtcgtctgg aacatctttc
     5401 aacacaacgg cacctgcacc gattttggcg ttttttccga tcgttaccgg tcctaatatc
     5461 tgcgcattcg cagagagcat ggctccttgt ttaacagttg ggtgtctttt acccgtttct
     5521 tttcctgtgc cgcctaatgt tacgccatgg aataaaatca catcgtcctc gatttctgcg
     5581 gtttcgccaa tgacgattcc cattccatgg tcgatgaaca cgccagttcc aatcgttgct
     5641 cctggatgga tctcaacacc agttaggaaa cgccagaatt gagcattgat cttcgccaat
     5701 aaaaagagcc ggtgctgata taaaaaatga gagaaacgat gccaaaacaa ggcatgaaaa
     5761 ccagggtatg tcaaaacgat ctcagctgtc gaacgtgcag ccggatcgtt tctctttgct
     5821 gcatcaatcg ctcgggtaaa ccaactcatg actttccact cctttcgtaa gaattttatg
     5881 taggaaatca tcttcttata aagaaaagaa gcgcctttta aataagtttc cttatcaaaa
     5941 gacgctttcc gcgtggttcc acttttcttc ataatcaatg attatctctg acagctaacg
     6001 cctgttatac gttcttggct actttgtttc ctataaaaca gtttttcctg ttttctgtgt
     6061 ttcgccaaga ccactcccag aggcatttcg gcaaccattc attgatacct tgcaccaccc
     6121 ggtatctctc ttgtaatgat cagctgctta cttttttctg ttcatcgtgt ttagttcagt
     6181 acttgattta aatgtgctaa tgatttttca cgaccaagca attcgatcgt ttcaggcaat
     6241 tctggaccat gcatttgtcc tgatacagct acacggattg gcatgaaaag attttttcct
     6301 tttacgccag tttctttttg aacagctttg atggcagctt tgacagttgg agcatctact
     6361 acttccattt gttctagctg tgctttgaat gcgtggagaa cagtaggaac tgtttcacca
     6421 gcaagtactt cttttgctgc ttcattcaat acaggatgtt cattgaaaaa tagcgtagac
     6481 agatcaacga tttctgcagc atagctcatt tgaggttgat acaggctaac gatcttcttc
     6541 acccattcga ttgtcttttc atctgggttt tctgctacac gtccatcagc gattagataa
     6601 ggcagacaca tgtcagtaaa tgcatctaga tccattgctt tgatgtaatg gttgtttacc
     6661 cattgcagtt ttttcgcatc gaaagctgcg ggtgatttac ttaggcgttt tggatcgaac
     6721 attttgatca attcttggtg ttcgaagatt tcgtcttcgc ccacaggtga ccagcccaat
     6781 aatgcaataa agttgaacat tgcatctggt agatagccta attcgcgata ttgttcgata
     6841 aattgcaaga ttgattcgtc acgtttgctt agttttttac ctgtttcact gttgatgatc
     6901 aatgtcatat gtccgaaggt tggtggtgtc cagccaaatg cttcatagat catcagttgt
     6961 tttggtgtat tggcaatatg gtcatctcca cgtaaaacgt gagtgatttt catcaaatga
     7021 tcatcgaccg caacagcaaa attataagtt ggcataccat cgcgtttttg gatgacaaag
     7081 tcgccaccga tattatcgga ttcgaacgaa atagcgcctt tcaccatatc tgtaaatgcg
     7141 tattctgtat tgcgaggcac acggaaacgt acaacagatt ctagaccttg tgcttctttt
     7201 tctgcttgtt cttcaggtgt caagttcgca catgttcctg cgtaatgagg catttctcct
     7261 cttgcgcgtt gtgcttcccg ttctgcttct aattcttctt ctgtgcaata gcatttgtat
     7321 gctcgattgc tagcaagcag ctgatcaatc agcggttgat agatttcttt acgttcagat
     7381 tgacggtaag ggccgtattc tccaggattt tcaggagatt catcccaatt cattcccaac
     7441 caagcaaggt tttccaactg gcttttttcg ccatcttcga tattacgttt ttggtcagta
     7501 tcctcaatac gaataataaa gtctccatca ttgtggcggg caaacaaata attgaataat
     7561 gcagttcttg catttccaat atgcaggtgc ccagttggac ttggtgcata gcgtacgcgt
     7621 actttcgtca ttttcttcct cttctttcaa actagacttt gactaacagc aaaaattccg
     7681 tcagttacgg cttctcaaat atcatttatt taaagccact cagcacaaat tttacctttt
     7741 ctaaggtaaa tagtaaagcc aactggttga atttccttac cagttggctg aatttctttt
     7801 ttattttgat tgtttagtct ttttctctgt ctctttttta ttgtcgattc ctcgtcctgc
     7861 atgtgcaggt ttagcaaaga tcatgcgtcc agcagctgtt tgaagggcac ttgttacaac
     7921 gacttcgata cgttcattca tgtaatgctg tccatcttct acgacaacca tcgtgccgtc
     7981 atcaaggtag gctactcctt gttggcgttc tgtcccggct tttactacta gaacattcat
     8041 cgtttcacca gggatgacga ctggttttac ggcatttgcc aacgcattga tattaagaac
     8101 aggtacattt tggaattcag agactttatt caagttatag tcattggtta caatgacacc
     8161 atctaaaagc ttagccaatt tgatcaattt gctgtctact tcactgatat cttcaaagtc
     8221 tccatcgtac atttcgacag atataccctc ttctttttgc aaggcattta ggatatctaa
     8281 tccgcgacga ccacgcacac gtttcaaact atcccctgag tccgcgatgt attgtagttc
     8341 gtaaagaaca aaattaggga tcatcaaggt tccttctaag aatccagtct ttgtaatatc
     8401 ataaatccga ccgtcaatga tcacgcttgt atccaagatt ttatatttat ggaaattttc
     8461 tcctgatttt cgttctaaca gttgactctc aacttgacta gctgtttctt gctcgatttt
     8521 tttgcttcgt gaaccaaaaa ctttcttcca ttcttcgatt cgtgtcgttc ccattctcaa
     8581 tcccagatag cctaagatga tcatcagtaa aatcggcaat acactattga cgaatggaat
     8641 ttccatattg taaagagggg tcgagatcac aacacctaaa gtcaatccga tgatcgcacc
     8701 gatcgcaccg aagagaagat aagtcaagct gacctcactt agtttttgtt ctacttgttt
     8761 gataatagct gagatatgtt tggccaacag caaagataat aaaaagaaaa taagtgcgcc
     8821 taacaggctg ttggtaaatg tattattcag ccatccgttt gactgttgac cgattgcctg
     8881 ccaagctgag ggtaaaaatg aaatacccaa gcttgcgccg acaatgatca tcagcaaagt
     8941 aatgacacgt ttttgcatcg tttttcctcc gtttcgacaa tcatttatta gttagcggaa
     9001 cacacgcttc aacgtttcag ccaaagttgc tactccaact atctcgattc cttttggcgc
     9061 ttcccagtta cctaagttgt ttttaggtag gtatactttt gtgaagccga gtttttggac
     9121 ttctttgact cgttgttcaa tatgactgac acgtctgatt tctccagtca gtccaatttc
     9181 accgatgaaa cattccgttg gctgtgttcc tttttcttta tagcttgaag caatactgac
     9241 agcgatagct aaatcaatcg ctggttcatt taattttacc ccacctgcag cttttaagta
     9301 agcatcttga ttctgaagaa gcaacccagc tcttttttcc agcactgcca tgatcagaga
     9361 tacccgattg aaatcaagac ctgttgttgt tcgtttggca tttccgaaca tagtaggtgt
     9421 gactaatgct tggatctcta ccaaaattgg acgtgtccct tccattgcca caacgatagc
     9481 agagccagtt gcaccgtcta ggcgttcttc taagaaaatc tgtgaaggat tagcgacttc
     9541 ttccaatcca tgctcatgca tttcaaaaat cccaatctca ttcgttgagc caaatcggtt
     9601 ttttactgct cgcaaaatcc gaaaactatg atgttgttca ccttcaaaat ataacaccgt
     9661 atccaccata tgttctaaca ttcttgggcc ggcgatcgag ccttctttgg taacatgacc
     9721 gacgataaaa atggctatcc catttgtttt agcggttttg agtagttctg cagtggtttc
     9781 tcttacttgg ctgacacttc ctgccacact tgtgatgtct ggctgcgtca tcgtttggat
     9841 ggaatcaatg ataacataat caggggagat atgttcgatg gcacgactga tttcgttcat
     9901 atctgtttca gcgtatagat aaaattctgt gttgatactt ccgaggcgtt ccgcgcgtaa
     9961 ttttatctgc tctgcgcttt cttcgccgga tacatataag acttttccgc ctatggctgc
    10021 taattgctga gaaacttgta acagcaaagt ggatttcccg atacctggat cgccaccaat
    10081 caagaccata gaccctggca ccacacctcc gcctaacaca cggttcagtt cttctaattt
    10141 tgttttgatc cgaggttctt ttttagggac gacgtctgcg atttttgttg gttttgcttt
    10201 ttctcctgtc aaacttgtcc gagttctgcg gtccgttgta tcttgttcga tttcttcgac
    10261 catcgtattc cattttccgc aatttgggca gcgtcccaag aacttaggcg acacataccc
    10321 gcaattttgg caaacaaatt gtacttttga tttttttgcc attgtaccgc ctccttttat
    10381 cctaatcatg agacattaaa tcaagcttaa gtatctttaa cgttttattt actggaagaa
    10441 ccaaatccgc cttgacgttg agcagtcgtt tgatcttgat ctgcttttaa aaacggcaaa
    10501 aagatccctt ggccgatacg ctcgcctttt ttgatcacta catccttgaa accaaagttg
    10561 gtgaattgga acatgatatg gccttcattt gcctcattat tgtaataatc actgtcaatc
    10621 acacctactc cgtttgtcaa cactaaaaac cgcttcaacg gattgcttga acggttagca
    10681 agctgcaaaa attcatcttc tcccatgtaa gctttgattc cagtaggcac gagggttggt
    10741 ttcaattgtt tttcattttc tgatacatct tttttcaatc catctgcgac tgtttccaac
    10801 ccctttttcc agcctgcttt ccagatactt ggaatcacga cctcttctgc tgcttcaaaa
    10861 tcatagccag cagcatgatg tgtagcgcgc tgcggtagat tgatattttg atctttatat
    10921 ttagaaataa cttcaaatcc tctttttctc accggttttc ctccctaaat aaactactcc
    10981 tattctaaca tatccgaggc tgtaaaagac tttctttaca aattcttcaa gaaattctgc
    11041 tatcttaaag taaaccatta attcgttctt tttccaaacg actgcaagtt gtacaaaaaa
    11101 actatttttt acatgacttt actcgtcaaa acaagtagaa tagaacgaaa ttaatgagtg
    11161 gatatttgat tgaggtgtca catgcgtaaa gaaattaaaa atgcaaaacg aattattgtc
    11221 aaagtaggaa caagtacatt gatttatccc aatggaaata tcaatcttgc ggcaatcgat
    11281 cagttagctt ttgtcctttc tgatcttaga aatcggggaa aggaagtgat cttagtctca
    11341 tcaggagctg taggcgttgg tatgcataaa ttacatttgt cagaacgtcc gactttgatt
    11401 cctgaacagc aagctgcggc ggctgttggt caagccgaac tgatgaatat ctataatcaa
    11461 agattcagtg tatataatca acagacggca cagatcctcg tgacaagaga cgtggtagat
    11521 tatcctgaaa gtcgtaaaaa tgtgaggaat acattggaac agcttttgat tatggacacg
    11581 atccctatcg tgaacgaaaa cgatactgta gcagtggatg aattagatca tctgacaaaa
    11641 tttggcgata atgatcagct gtcagccatc atggcaaaac ttgtcgcggc agaccttttg
    11701 atcgtccttt ctgatatcga cgggttttat tcagataatc cgactacaaa tcccgaggct
    11761 cagatgtatc gctatatcga taaaatcgat gaaaagattc tggcacaagc aggtggtgct
    11821 ggtagcacat ttggaacagg cggtatgcag agtaaattaa aagccgcttc taggatattt
    11881 gaagcaaaca gcgcaatgat tttagcaaat ggaaaaaatc cggcaattat ttttgatatt
    11941 cttgcaggaa aagaagtagg tactttattt aaggaggact tgaaatgact gacttgatgc
    12001 aattaggaca acaggcaaaa gatagcgcgc aacaactagc acttatgcct acaaaccgca
    12061 aaaatgagct acttgttcaa atggcacaaa ccatcaaaaa gaatcaacaa gcgatcattg
    12121 aagcaaatca taaagactta gaaaaagcag tcgaaaacaa tatcagtgaa acgatgcaag
    12181 accgcttgca gctgacacct gagcgaatca atgccatggc tacagagatc gaaaaaattg
    12241 cttcgttaga cgatcctatt ggaaaagtag atgagatgtg gacgaacacc gacggattga
    12301 gaatcggcaa aaagcgcgtg ccactaggcg tgatcggcat catttacgaa tctcgtccaa
    12361 atgtcacgac agatgcagct agtctagcat tcaaatcagg gaatgccgtt atccttcgcg
    12421 gtggcaaaga agcattcttc tcgaatcaat tattagtcca attattgcag caagtactgg
    12481 tcagtgctgg cgaatctcct tatgccatcc agtttgtcga tgatacttct catgaaactg
    12541 caggacagtt gatgcgattg acagaatact tagatgtgct gattcctcga ggcggagcaa
    12601 aattaatcca acgagtcaaa gaacaggcaa cggtacctat cattgaaaca ggtacaggaa
    12661 ataatcacgt atatatcgat aaagaagcac agttatctat ggcagtgaac atcatcgtca
    12721 atgcaaaagc ttcccgtcca tctgtctgta acgcggcaga aacattgctg atccattctg
    12781 agatcgcacc ttttttcttg ccagctattg aaaaagaact ggtagaacac ggggtctctt
    12841 tacgtgcgga tgctcgtgcg ttagaatatc tagagacggc tgctttagca gaagacacag
    12901 attgggatac tgaatatcta gattatatcc ttgctgtcaa agtggtcgat tctttggatg
    12961 aagcaattgc acatatcaat cggcataata ctaaacattc agaaacgatc gtgacagata
    13021 gttatgcggc tagtcaacgt ttcttaaatg aagtggacgc ggcggctgtc tatgtcaatg
    13081 cctccactcg ttttacagac ggatcggtat ttggttttgg tgcagagatt ggtatctcta
    13141 ctcaaaagtt gcatgcccgt ggtccaatgg ggttaaatga attaacttcc acaaaatata
    13201 tcatttatgg cgatggacaa atccgcgaat aatgtttcgt gtgaaacatt attcccgcct
    13261 tttgcctttt ttctgtaaag gttctatcca actagaaagg tgtcaatatg tttgaaattt
    13321 taatgatttt ttttatttac cttatttctc tgaatatcgc tgcttttctg ggagtcggta
    13381 ttttatccct tttctttcaa ttcaaaaaac gaagcatagg gagtcaacgg gaaaaatggt
    13441 cgcagtattt tgacaaaatc gggcaaaaag ggctggttac acgcttgcac attagttaca
    13501 tggttgcatt atgcttatta gcgacaatca attactattc ttttttcgat cattcaatcg
    13561 cttatacgat tacgctactg attgctggga tttttcactt atcttataaa tatcagctga
    13621 ataaaaacca cttgaatcgg actttccgct aaaaaatatc cgtacgataa gtagagtgcc
    13681 ttgaagatcg gcaaaaaact gccggacttt ttagaggctg tgacaaagat tttgtcacag
    13741 cctctttttc catataaacg gcgttcgcct accagctgcc tggtaaatct gtttcctctt
    13801 cgttaagaag cgaaaatccg tcaaatgttt cgccatcata ataacgaagc agctcatcca
    13861 tggacaagtt catcgttttt tctttttctt cttccgctga ttcagccagt agctgttccc
    13921 gatacacctg atacatacca gctatttttt gctgcatcgc ttttctagtt tcaccttccg
    13981 cattgattat tggcaattct ttgatatagc cacgataaat caaccgatca tctgaaaatt
    14041 caatgggtaa ttcaacgagt gtcaacccga cttcctgaaa atattcttcc agcatctatt
    14101 atccccccat tctttttctt aaatgaattt tagcttacct actgaaaatt ttccagtttc
    14161 attattttga gaaaaaaagg gaatgtgaca aaaatcgtgt cacatcccct ttttccgaat
    14221 aaatggtgtt cttctgccaa ctcctcaatc ataagtcaca atattcaaca aacatgagaa
    14281 actgtttttt gaaattgttc cttgtgattc agagttaaac gacagtctca caacctcttg
    14341 gtaaacggcg ttcaatcagc tgaccctcgg tattagctca aaaaacggta aaatatgaga
    14401 agctattttt ccgttttgtt tctctaatac ttaggataaa acgctgattt tacaacctct
    14461 gttaaacggt gttcaatcag ctgtccttcg gtattagctc aaaaaacggc aaaatatgag
    14521 gaactatttt tccgtttgtt tctctaatac tcaggacaaa acgctgattt tacaacctcc
    14581 tgataaacgg tgttcaaaag cttgcatctg cggtaataag cccaaccaaa cgaaaaatat
    14641 gagaagctat ttttgtttgg ctgttcttat gacttgatgc aggacagctt tttcacaacc
    14701 tctgatttct tattcttctt catttgtagc aaaataatta cggtgataaa tgatacaaca
    14761 tacactgata agaacaatgg cacctgcaaa ataaaacgca tagctttcac caatatgttc
    14821 accaatcaca cctgcaataa atggtgctaa agctccaccg aaaaagcgga caaaactata
    14881 gctggacgaa gcaacgtttc gttgcagacc tggtatttcc atagcaatcg tcgtcaacag
    14941 tgtattgata attccttgga aaaatcctgc taacacaatg ctgatcggta caaggactgg
    15001 catagaagtt ccagctccta ataacagcaa acaaataatg aataagacaa atgctgctaa
    15061 aattgtccga taagtcgtaa acttccgctc aagagccggt gcaacgaaaa tcgaagcaat
    15121 agccagaagc acaccccagc caaaaaatac aaaaccgact tctaattcag aatagccaat
    15181 caataaaaat ggcgcatacg caagtaaagt aaagaatccg aaattatata acaatgcgat
    15241 tagtccaact gaacgaagtt tatgattgct taacgctttg acaccttccc aaaggcctac
    15301 ttttctcttt ggtttctcag tttcaggtaa aagtaccgct agggctataa aaccgcaaaa
    15361 catcaaaaat gctaccccaa aaaatggata acgccatgaa ccactgccca agaccccacc
    15421 gactagaggg ccaacagcca ttccgcagcc aagtgctgct tcatacatca tgattgcttt
    15481 ttctgtgtta ccaactaaaa tactgacgat cgttgctaaa gcagtagaga caaataaagc
    15541 attcccgaca ccccagccgg cacgtaaccc aatcagcatt ccgatattcc cagataatcc
    15601 gccaagacca gcgaatacaa cgatgataaa caatcctgtc atcaatgttt ttttcaaacc
    15661 gatccgacta gataagaaac ctgtaaatag catcaccaca ccagtgacta acatgtaact
    15721 agtaaataac agggtcgttt cagctggcgt agcgtttaac tgtgcagcaa tcgtttttaa
    15781 gattgggtct actagaccca ctcccataaa agcgatcata caggtagctg ctacaactag
    15841 cgctgaactt ttcccctttg tttcttcatt tgtttgttct tgtatatcta tttgattctc
    15901 caatatttcc atccctttct ctctattagc atcatacatg atttatttta catgtcaata
    15961 tgcacatgta aaacgtggaa actttactgt gatataataa agaaaataag acgagaggta
    16021 ggatttttat gaatacttta gcctatatat tattatgcgt gcttttatcc aaaccacgtt
    16081 ctgggtatga attgaagcag ctgatcacaa ttttttggga agcccatcac agtcagatct
    16141 atacaacgct tgccaagtta gaccagcagg gttatgtgac cgttttggac aaagacgagc
    16201 actctcaaaa aaagatctac cacttgactg atgaaggacg tgctcttgta gaggaatgga
    16261 tcaaagagga aactcccgct cctacgcaaa aagatgaatt tctagcaaaa atctatgctt
    16321 tttcgacgct cgataaaaca acagctactg gactgctatt cactagagaa caacagctga
    16381 acaaaatcat gcaaaaaaat caaaaaaaat tgaatgacct ctcgctagac aagaaacaag
    16441 aatttggacg ttatgttgtg atccagcgca gaattatgct ttgtcagcag gaactccaat
    16501 ggtgtcagca agtaagagaa caaatgcagc agttttttga gtaagggata aacagaacct
    16561 ttctagaaat atctattgaa gagcaaatta tcttcaataa aagagcaata cagttggaaa
    16621 agaaaaagcg atccgaacga caggccaaaa cctatcgttc ggatcactat ttttctagaa
    16681 ctgcattcaa gagactatga caaaaagcat gccaaggcct tttttcaaat aacagtgttc
    16741 catcattcat tactttgaat tgcttttagc acacttcttg aaagcgggcc aacgatgatc
    16801 aactgcaacg gtaaagcggc aatcacattc attttccaag cattcatata agcagagaat
    16861 aattgatcag gtattccttg ttccatgatc acaccgaata aagacataca tgtcaccatt
    16921 cctaaaacca tcaagcatga aatagttaag atcatttgga ccttgctttc tggatttact
    16981 ggtagtttaa aggcaatttt tttcgccagc acaccgatga tgaagacatc caaaatgaaa
    17041 gccaccacga atccaggcac tagaccaatc aacaaacttg ataaatgaag atcttgatgc
    17101 agccataaat tatacgcgct catccccaag accatcaaaa aacacatcat tgttgtaaaa
    17161 attattcctt cttttttatt tgtaggcaat ctgtaccact cctttaaatt tgtcacgaaa
    17221 atcatcgtag cacatttaaa aatttctcgt aagtactttt tttattttct gcccttttat
    17281 ttcataaact tcatcagccg tacgctcgac aaagtaagaa tcatgagaag taaacaaaat
    17341 cgttcccgga tatatttgca ttagcttctc taaagcttcg atcgttgcca tatcaataaa
    17401 attagtcggt tcatctaaca gcaacacatt acttggctta gtaaaaagca aagcaatcgt
    17461 cagacgggtc gcttctcccc cacttaatgt acataacggt ttcgtcaaag tttcctgagc
    17521 aaaccctaag tgattcagca aactacggac aagcgattct gaaaactctg tttgtctcat
    17581 gacaaaacga atgatggatt cttcagatgt catttgataa cccttttgct gatatacttg
    17641 aaagcttact ttcggagaga gaaggattcc ttctccttgt ttgcggatat gttctaataa
    17701 ggttgtctta cctgaaccat tttcaccgac aatcgcgatt tttttaccta aagaaaactg
    17761 aaaatcacca tttaccagta atgttctccc acttctttcc aattggacat tttgtcccat
    17821 gatgggatag cggctgtgga gttcaagaga ttttggtact ggaaaacgga tttgtttcat
    17881 ctgctgcggc ttggttgttt ctcctatttt ttggagtctt ctctctaatg ttttcgcatt
    17941 cttttggatg gctttttgta cactgtcttt ttgtttagag gaagacaacc gatccagtct
    18001 gatttgttgc tttttctttt tactggacac tttttccaac ttttccgctt gttttttctt
    18061 ttcttggatc gatttttcca atcgtttttt ctctttcatg atcgactctg cttctcgtag
    18121 ctgagtcttt ttctccaatt ccttttgagt gagataggca ctataattcc ccgaaaactc
    18181 tcggatttct ccgtctttta cttcccagat tttcgatgct agctcatcta gaaaatagcg
    18241 atcatgacta acaaaaacca gcgtaccata ataataacga agctcttcaa tcagtcgatc
    18301 aatacttttt ctatctagat gggttgttgg ctcgtctaga agcatccctt gttcataaga
    18361 agaaagaaga cgtatcaact cgatctttcg ttcttcccct ccgctccatt tccgctctga
    18421 agtttcttcc agtcttaact ggaaatgact ggctaattcc aaatttaggt cctttgcttc
    18481 agcgagatag gttaattgag gcaggtagtt gaaggtgatc tctgtttgga ttttcccatg
    18541 atcgggaaaa agttcaccgg ctattagttt gagcaaagtc gatttaccga caccattttc
    18601 tcctatgatc cctatacgat cttgctgata gacacgaaga gaatcaatag atagttctag
    18661 ctggttgcca aagctgactt gtagatttgt tatatttact gctaaatttt ccatatgttt
    18721 tacactccta tttcatgtaa aacaacaatt atcgatacta aaaaagagca gaaaaatctg
    18781 tttgcaaaaa aactgcatca catcttttcc tactctgaac gaaataacta tgactaaatc
    18841 accaaatctg tcatagcttt cacctattct actgtcgttc gaaaaaatgg acatactcca
    18901 tccatgaaat tgttgtttat acatgtcttt tgaatttatt tagttgttat tcaaaagttc
    18961 gaaacgtaat ttcatcgatg cagtcatttt tccgacctcc ttttttatta ggttaccaca
    19021 cacgattcaa aaaaagcaat agctactgaa tagtaactga aaacctttta ttcgaacata
    19081 gtgatctttt ctggtgctaa acgaacagac tcgtaacctt cttctaccgc ttttcctatt
    19141 gataaaataa taactggaac ataacggtct ttatccaaac caaatgcctc tgccaactga
    19201 tctgcttcga atccaccaat tggatttgtt tcatagccat gatcacgcgc aactaacatg
    19261 aattgcatag ccgctaagct agaatcgact ttgactacat cgttcatttc ttctcttgaa
    19321 aaactcttgt aataaggaat gatcgcagct aattgttgat ctctgacttc ttttggcatc
    19381 ttccctgatt cgtaagcttg gttgtagatt tcttcgccat attcgtaaca ttgcatatcg
    19441 ccaaaaatca tgaccattgc agaagaagta tcattttgtc tcgtattgaa acggatcaaa
    19501 ggacgcaaga tttcttttgc ttcctcgctt tcagcgacca caaagcgcca tggtttcata
    19561 ttgacagaag aaggtgcagt cgtcgcttct ttgatcattt ccagcatttc ttcgtgtggg
    19621 attttgtatg tttcatcata tacgcgaacg gatcttctac cagtcataat tgttgaaaaa
    19681 tcattttttt gttccattat tattaacctc tttcgtttct gttgttatat tgcaaagttg
    19741 ggtattcaat ttatctaaaa gtgttagaaa ttgtttcatt tctacatcgc tgaaagccgg
    19801 ggatagcacg gaacctgtat ctttatggtc catttcacaa tgcttgattt tcttctgggc
    19861 tttttctgtt agcgctacat acacttcccg gttgttctga ggatttctct gacgcgaaac
    19921 atagccattt gcttccaata ccttcagatg acgagtaact gctccttgat caatctgcaa
    19981 cttttcttgc aaactgcttt gtagcaatgg attttcttct ttcagtctca tcatgatctc
    20041 gtatctagta agacttatac cgatattttc ttcaaagatc tggacgattt tttgatcggc
    20101 taatttgatt tgatataaaa ggtgactaat ctcttctaaa gacataattt cctcctttct
    20161 aataattgac tcgtcaacga ttgacttgtc aataatagaa taaaaaaaga aaaaagtcaa
    20221 agaatcagct gtgtaataaa aaaaacgctc tcaatagaaa agtatagaga aaagaaattt
    20281 tttgtagaat gaacggaaaa aatgataaag aaagcacaac gctaaaacca agattattga
    20341 ttcttatcaa actatagaag atagctcttc tgcagtagat aattttacac cgccaaaaat
    20401 ttccgaaatc gccttttgca agaatcccgt cgaatattag aaattggatc agtatgctta
    20461 aacagtaata aaaaagttat gacaagatgt tgtcacaacc ttttttgagt aaacggtgtt
    20521 caaaaacttg catctgcggt aataagttca accaaacaaa aaacatgaaa atctgttttt
    20581 gtttggctgt tcttactact tgatgcaggg cagctttttc actacctctt tttaataacc
    20641 ataaaatcct attccttcat atctccagcc catcggtgtt agccaatctc tttccttagc
    20701 actcaaggta tagtgatggg taccagtttt ttcatttgga ttaaatagtc gataaattgg
    20761 cttggaacca ccagaataaa aacttagacc ttcataacgc caaccgattt ttgttaaaga
    20821 gttgatctca gattgtgcca ttgtgtaatg atggtctcca ctgtaaggat tgtataaacg
    20881 gtagacaggt tgtccgctgt ttggtgcttg ccaagcaatc ccttcatatt cccagcctac
    20941 agttggcaga taattttttt ctccggcatt caatgtatat aaatgttctc ctgtatgacg
    21001 gttatacaga cgatacatcg gagaagttga agttggtgct gtatcatact gtgtcagatt
    21061 ataggtttcg attacattgt tcaatgtaga accataatta ggcgcagtgg cataacgacc
    21121 tgttagccaa gccgtagcat ctttgtaaga gttcgtattg cttttccatg caccggaata
    21181 gtagtaaacg cctggctgaa aagaagtcgt cttcaatacc gttacgttat cttgcaatga
    21241 ttctttgtac gaaggatatt ttctaaaagc ggcattcacc gtaatccatt gcccattcac
    21301 atattccgaa gtaggcatcg taacagattg gccattatag cttcctttaa tcccaaatag
    21361 attgtaatta ggggcagaag ccaaagtact gttcccccag ccactttcaa cgatggcttg
    21421 agcaatcatg actgatgcat ataagtcatt ggcacttgcc aaactttgtg cagttgaggc
    21481 aatactatca atgaacgctt gttgagaatt agcagatgag cgagcataaa aagcatagcc
    21541 agtatagttt tcgaattcag acaaatctgc ttgatgggta tgtgtcaatg tttgcccatc
    21601 tgtcaattgg ttcgtgattt cttctgcatt ctctggattg ttctgagctt ctgctgatgt
    21661 actgtcttcg gtttgtgttt gttctgttgt ttcagttgtt ggttcttcgg ttgaaattgt
    21721 attttctgag acagtagctg actgcaaagt actttctgta tcgttttcaa ttcctgtttc
    21781 tgaagaactc gatgtctgag ttgcgacagt ttctgtcgat tcttcagcta gcactgttgt
    21841 gttggcaact gctagatttc ctaaaagaat aacgctaaat aataaattct ttgctttcat
    21901 cttttttctc ctttgaatta tgaaactttt cctaaataac agtgtaacta aaaaagtaaa
    21961 agcaagtcaa tgtccatatt taaataacat ttgtttcttt ttaataaatt tgctttttct
    22021 gcataaaaag acgtaaatga agaatattct tctccattca cgccttaaat tgatggtcgt
    22081 ttgaataact atttaacaag taattctctc tggatattat ccgttatgga ttatacttcc
    22141 tgtgtcgtcg gacggataat catgttgctg acagacatcc ggtctggcgt ttctatcgca
    22201 aatgctacag cagaagctat atcttcagat gttaatcccc attctttttg cgcttcatga
    22261 agttcaagag cagccttttt gtcgctgatg gtttgatata attcagtttg aacagctcct
    22321 ggagagatga tcgtagattt gatgttattt tgccgttgct cttgacgtaa tccttccatg
    22381 attgcacgga cagcaaattt tgttccgcaa taaacggcgg aatcaggata aacaacatgt
    22441 cccgccacag aatcagttga aatgatatgc ccagattttt gtttttccat gattggaagt
    22501 acggcggaaa ttccatttaa cacacccatg atgttgatat caagcatatt ttgccattca
    22561 tcacggtgac cttcaactaa tggagcggtt ggcatgattc ctgcattatt gtacaataca
    22621 tcgattcgac cgaacttttc catcgctaaa tcgataactg cttggacttg tgcaaaatct
    22681 cttacatctg ctgtttgaat atatagctca gcttctggta aagattcttt tattgctttt
    22741 aggcgatctt ctcggcgtgc agctataact agtttagccc ctttttctgc taatagcttc
    22801 gttgttgctt caccgattcc gcttgaagcg cccatgatga caatcacttt ttctgataaa
    22861 tcattcattc tattctcctc ctgttttgaa aaatctcttg cttatactca ctatctctta
    22921 tttcatctta ctgttcaaat aatccgttcg acaggtttga cgattttggc agggattcct
    22981 gcaacgatgg tattgtctgg gacatctttc gtaacagttg cattcgctgc aacaatggca
    23041 ttctcgccaa tcgttacgcc tggcagaata gttgctcctg ctccgatcca agcatttttc
    23101 ttgatgtgga ttggctttac tgacaagcct cttcgttctt ttggatcggt tagatggttc
    23161 accgtgatga ggttgacacg aggaccaatc aatactgaat cttctaaaca gatgccacct
    23221 aagtcaacga acatcacttg acgattgata aagacatttt tccctatact aatatgtttg
    23281 ccgaaatctg tataaaaagg cagcagaaca gtcaccgtat catctacttt gtcactaatg
    23341 atattttcta gccgtttgcg gatatctgct ggttcatggt acccagtatt cagcgctgct
    23401 aaaggctctt cgttttccgc ttggatctga tggatcactt caaagagcgg atccgttact
    23461 cgaatctctt tattgacgat ttgtttttcg atgaggcttg tcattttgct ccctcctttt
    23521 atccttactt tataacggac ggcttatgat gtctaatact tataaaacat acttaactat
    23581 tccttttaac tatacttagt ggtatgcttt tttaaaaaca ggagggaaaa aatggatatt
    23641 cgtatactga cgtatttttt agcagtagct agagaaaaaa atatcagccg agcagcaaaa
    23701 tctctgcata tcacgcaacc tactctctca aaacaactga aagatttaga agaagaactt
    23761 ggtgtgacac ttttcactcg aggctcccga gagatacagt tgacggattc tggtcgatat
    23821 ctttattcaa aaggaaaaga aatcttgtcg ttggtagaaa aaacggcgaa caatttaaca
    23881 aatgacgcaa tcatctctgg agaaatctac attggcggag gcgaaactaa agcaatctct
    23941 ttcatttctg atagccttca ttcgttgatc aaggcgcatc ctgatatcca gtttcattta
    24001 tatagcggga atgcagatga tatcatagac aaactggata gcggattact tgattttgga
    24061 ctcgtaatcg aaccgacaaa caaacaaaaa taccagtcat ttcgactgcc agcccaagat
    24121 cgttggggac ttttgataca tcggaatcat cctttagcaa agcaaacagt catccatccg
    24181 caggaactga aaactgttcc tttgatcatt tcccggcaaa cgtcagtgga cagtcaacta
    24241 gctgaatggc taggtttcag catagatcag ttatcgatcg tcggaacata taacttattg
    24301 tacaatgctt ctttgatggt cgataacggg acttttggtg cattagctct agatgggatc
    24361 atcaatactt atgggacgga aaaagtattc atcccactcg atccgccatt acaagcttca
    24421 ctgaatctca tttggaaaaa agaccagcct ctttctcctg ctgctcgtaa atttttagat
    24481 gtcctaacgg agaaatacag ttaagtagag tttttttcct tcgctttcag ctttttaaca
    24541 acgaataagt taagcaaaag atccaatgat taaataacat aagtttctta atttgttcct
    24601 tccttttatt tctgataatt ccagtaaatt actataatta taaaaaaaga attgacaaaa
    24661 cataactgtc tatataaaat tgattacgtt aacaataatt cgtttcatgt gactagatca
    24721 tactaggcat gaaaaatctc tgaggaacat ggtttcccta ggaggttttt cgtgtctatt
    24781 tttgtttggc cgcaatcaac tggtctagca aacgtgaaaa ccaaatcttc aaaaagggtg
    24841 cgcgcatgaa aatcaaaaag atctttaatc aaaatgcagt attggtagat gacggtggac
    24901 aggaaaaagt agctattgga aaagggattg gatttgacag aaaaagaaat gatttgattt
    24961 ttgatcgtga tgtcgaacgg atatttgtca tggaaccaga aggtcaaatc aagttgcaaa
    25021 atttacttaa tcagattgat gaacgctttt tatttgcagc agaaacgatc attgatcatg
    25081 cggaaactgt tttgatggaa aagttgaatg aacatgtatt gatcgctttg accgaccaca
    25141 ttgctttttc tgcggagaac attaataacg gtatcgtcat ccgcaacaaa cttttaagag
    25201 aaatcgaagt gctttacagt gatgaattca gtattgctca atgggcagta gagtatctga
    25261 caaaagagct tgggattcca tatacttatg acgaagcagg atatatcgca atccatattc
    25321 acagcggacg aagcggtcgg aacaataacc atcgcagtat ccgtgaagtg acgattgtat
    25381 ctgacatcat ccatttagtg gaatcagaat tggaaacaga tatccattca gaaccatttt
    25441 cactgaatta ttcaagactg gtgaatcatt tacgactgct tctacaacga acccatgcgc
    25501 aacaatatgc tgttttagac acggaaatcg tagaaatggt caaacgaaaa tacccagaaa
    25561 gctataagat ttctaaaaaa atccgtgtgt tgctgaccaa agaataccag ctcgcgatca
    25621 cgaaagaaga actgggttac ttggccatcc atatcgagcg tttaagaagc gcaatcgtac
    25681 aaacaaatgt taacaatcat gaggaggaaa aaaattgaaa gcatatctac aacgaatggg
    25741 acgttccctt caacttccag ttgcggtctt acctgctgct gctttactag tagggatcgg
    25801 aaactggtgg gcatcttata gtaacgacat tgtcgctcat tttttacaag ctggcggtaa
    25861 cgcagtttta ggtcaattac ctttgctgtt tgccgttggg ttagcactag ggatgtcagt
    25921 ggacaaatca ggtgctgctg ccttggcagg tttggtcgca tttgaagttc cagtaaatgt
    25981 tttaaaaact gattcagtag ctactttatt gaatatcaaa ccagaatcag ttgatccatc
    26041 ttttgctcag atcagtaacg tatttattgg tattattgcc ggattgattg cggcagctct
    26101 ttataatcgt ttccacgaag caaaattacc gatggcttta tctttcttta gcggcaagcg
    26161 tctagtgcca atcttggctg cctttgtcat gttgctgatc agtgcagtgc tactgctcgt
    26221 atggcctccg gtttactctg ctttagtttc ttttgctaaa ttcattttga gtttaggctg
    26281 gggtggtgca ggtctttatg gcttcttcaa ccgtttactg atccctacag gattgcatca
    26341 agcaatgaac tccgtgttct ggtttgacgt agcaggaatc aatgatattg gtaaattttt
    26401 ggcttctgaa ggaacaaaag gcgtaacagg aatgtatcaa gctggatttt tcccaatcat
    26461 gatgttcggg cttccagcag gagcatatgc tatttacaga aatgcacgtc cagaaaacaa
    26521 agcaaaaaca gcttccttga tgttagcagc agcttttgct tcattcttta ctggtgtaac
    26581 agaaccactt gaattctcat ttatgttcgt tgcttggcca ttatatgtct tgcatgcaat
    26641 tttcactggg atttctttag cagtgagtgc attcttccat tggacagcag gatttgcctt
    26701 tagtgccgga tttgtcgatt acttcctatc tttgaaaaat ccgattgcga atcagccatt
    26761 gatgttgatc gtccaaggat tagtaacagc agtgatttac tactttggct ttaactttgc
    26821 tatcaaaaaa ttccatctga tgactcctgg ccgcgaggaa gcagatcttt cagatgacga
    26881 tactgcaacg actaattcag acaataaata tgcggcacaa gcaagcaaaa tctacgctgc
    26941 attaggcgga gccgacaatg taacttcgat cgataactgt actacacgcc ttcgtttgca
    27001 agtcaaagat acaggcctga tcgatcaaaa taaaatcaaa gccactggcg tacctggtat
    27061 gaagatcatc gacggaaaaa atgcgcaagt catcgtaggt acagaagtac aattcgttgc
    27121 agatgagatg gcaaaactac atggtggtgc tgcaacacga cctgcacaaa caaacaccgt
    27181 ggttaaaaca gaaacatttg ccggtggaac gatcgaacaa gatatttacg ctatcgcaaa
    27241 tgggaaactg attccaatca ctgaagtttc agatgatgta ttcgcggaaa aaatgatggg
    27301 tgacggctat gctgtattac cagaaaatgg agaaatcttc tctccaatcg ctggtacgat
    27361 cacaaacatt ttcccaacga aacatgctgt cggaatccaa acagatgctg gtatcgaagt
    27421 cttgcttcat atgggcatca acactgttga cttaaaaggc gaaccattca ctttatatgt
    27481 aaaagaaggt caaaaagtcg caagaggtca attgattgct ttagttgatc ttgccgctat
    27541 tcaatctgca ggcaaaaaca cggatatggt cgttgtattc acaaatggcg acaaagtcca
    27601 aagcttagaa atcgaacccg cacgtgatgt aaaagcaaat gataaaatcg gctctgtttc
    27661 caacaaagcc tgagccatcg tcttccccag acttttttat cgataaacaa acaggagctg
    27721 agacaaaaat cttgtctcag ctcctttttc cgaataaaaa cggcgttctc ctgccaactc
    27781 cgcggtaata agtccagcca aacgtaacat atgaagagct attttttatt tgactcttct
    27841 tattacttga tgcagaacag cttttttaca acctctgata aacggtgttc aaaaaccagt
    27901 ttatcggtat gaagtcaaat ttatgcaaaa atgtggaaag ccattttcac aaaatttttc
    27961 ttcatactca aaactaatcg gctttttcac aacctcttta tacattattc ctgatccaat
    28021 atttctttta atgctgcttc aagagaagtt aatggttttc ccaatacttt ttctaaatca
    28081 tcagaggaaa tagctaactg atccttttta atatcatgtt ggatcgctaa aaacatgtca
    28141 gctgcttctt gagggaatcc ggcttctttt agattttcga caaacgctgc atcacttgct
    28201 tcaataattt ctaacttttt acctgtcgca cgctctaatg ccttggctaa ttcttcatag
    28261 tcagttagat tgccggataa ttctaagatt tctggagagt cactttctac tagtactttt
    28321 gctgcagctt ctgcatattc acgtttcaat gcccaacctg tttttccatt tcctgcagca
    28381 taaacgaatt gaccagtttt taaagcctgc ccaatgatag gtaattcatt ctctacatac
    28441 caattattcc gtaaaaatgt atgggaaata cctgaatcct taatcatttt ttcagtgaat
    28501 tggtgatctg gcgctaatac actgatcgag tgatcggcat ctgcaaaact tgtataagcg
    28561 atataagata ccccagctgc ttgtgctgcc ttcactacat tcttatgttc ggcttgacga
    28621 tttcctggaa cgccagagac aaaaagcaat cgatctattc ctttcaacgc ttctttcatt
    28681 gagtctaaat catcataatc accaatacgt atattaaatc cagcttcttt cagtgcttct
    28741 cctttttctt cactacgtac tagtacatag atttcatttt gtggaaccat cttttttaag
    28801 aattccagcg cataaccacc aaaacctcca gttgctcctg ttactaaata atgcatattt
    28861 gccactccct attcttttta tttgttaaga tttacagctg tacatttata aattacacct
    28921 ttatcataaa gagtgtggta agataagtca agcaaaaaaa atgaaaggat gacaactgat
    28981 gaagtattct ctgcaattta gtgatgcaat ccatgtactt gcctatattg aaatgtttca
    29041 agatacccga tcactttcta gtgagatgat tgccagtagt atcgaaacaa atgcagcaaa
    29101 tgtacgtaaa atcatgagtc agctcaaaaa atccggtttg atctttacaa aaaccggaaa
    29161 accgcagcct tcccttgcca aaagtccaga tcagatttct ttactagatg tctatcgcag
    29221 catcgaaggt aatacacagc ttatccaagt cgatccaaaa acaaatccaa attgcgtcgt
    29281 tggtgcgaat atccaagaag ctttagaaga aagctacgct cggctgcaga aaaaggcaga
    29341 agaagaaatg gctaagatca cacttgatag tctgatacat aaaatagccg ttttggaaaa
    29401 agaaaaaaga ccagaaaaca caggcctcgt agagagattt ttataattct ctctactgtg
    29461 cctgcatttt tctgatcgga atcgtctaca taaattcttt tattctccgg gggacaaaag
    29521 ccttgtcatg cccctttttc ctctctaatg ctcaggtcaa agcgctgaag tcatgacgtc
    29581 ttgataaacg gtgttcaagt agctgaccct cggcattagt tctgaaaaat gcaaaatatg
    29641 gaaagctatt tcccattttt cctctctaat gctcaggtca aagcgctact ttcacgacct
    29701 ctatattatc atggtttatt gactaccggt atccagattt ctcccatata tttcccgttg
    29761 gtaactccca tttcgactac agcatttgga cccccaacat aggcgtaatc ttctacttct
    29821 cttaaaacat gcccaaaggc cgtattcgtc aaatcatttc ttaattcttc aggcgattct
    29881 gctaaagcga ttacaacaag atacgttcct tttgggaatg ggatgattcg ggtagtgttt
    29941 ggaacatcct gactcgtttg aacaccgaca tagtacatca ttttcccgtt atagggttcg
    30001 ttgactgtaa agaaataatg attttcagca gcttgtttca atgcatctat tgttccatta
    30061 ccgataactt tttgctcaaa tgcttgtttc tctgcagcca aaccgacaaa atccgtattg
    30121 ctactcttca attccacacc aatacccgtc accgtaaaat ctggtttttc ttcgatccta
    30181 tactttgcca tttctcccac cctttctatt atattaagtt catcttacat aaaattaaag
    30241 ggaaatgaca cgattacgct cttttttcta gggatatttt tattttttaa ctattttttt
    30301 gctttcactg caatccaaat ctcactgtaa acattactga aatcctctcc ccattttgta
    30361 aaagagatct cgggagcttc gactaactca taatctgaag aaggaagcca ttctgcatag
    30421 attttccccc aagtatcttg taaagtttgg ggaaatggcc ctttattagg aaaaatggcc
    30481 caagtgtgtt cttctatagc aagcatttct aaatcagcgt acggattttc ttttgtcgta
    30541 gcaaagccaa tcatgtgcat caaactgccc ttttcttcca agcgggctcc atcaaatcca
    30601 taagaaacgt tgattacttg atttggatat aaatctccta acttatgcat ctcttccctt
    30661 tgagtctccg tgatcgtctg cgctaacttt tgtatttctt gattgactcc ttcaaatttg
    30721 acaggaactt ttttcgccac acctgccaca taaaactgtt ctttcttttc gatacgaaat
    30781 tccatcgatt ttcctcctct gacatcaata aaaaatgtca agcgtggaaa agatttttgt
    30841 atgccgtatc tgctgatttc tgaaggcaga tatcctgacc aatcacgaaa agctcttgaa
    30901 aaaccttcga ctgtttcata tccatatcta aacgccgttt ctgtcactct tgatccatct
    30961 aggagatcgc gattagccaa agccaatcta cggtttttga tatagtccat tagcgacatc
    31021 cctgcaataa acgtaaagat gcgttttaat tgatattcag agattcctgt attttcggct
    31081 attattttat aggaaagtcc ttctgtcaaa tgttcttcga tgtaatcgat caattggttc
    31141 agatctttaa gcatatttat cttcctcctt gcttgccttt ttatcatatt atcaaagaaa
    31201 gtcaattact tgatgaatca ggaaccaatt tagtcagatg tagaaaatgt tgtatggatc
    31261 taaagaaaat tctcaagcag cgtttcgtaa cggataattg gtattatctg cctatctatg
    31321 ttttaccttt agaaaaataa attttgggga agacacattc taagtaaaca aagcggtaaa
    31381 aatatctttt attcccttag ttaatccgct tactacctac tgtacaaagc aaattgttgt
    31441 tcaaactgct gatcagaacg ctgcttggat ttttctttgc gatacatcat acctctttta
    31501 tcaattggaa caattattta tttctaacaa aatcacttca tttttgtcag ataaataaaa
//
LOCUS       DAJSBL010000029        31245 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.029, whole genome shotgun sequence.
ACCESSION   DAJSBL010000029 DAJSBL010000000
VERSION     DAJSBL010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31245
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.029"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            202..1077
                     /locus_tag="ONT85_001597"
     CDS             202..1077
                     /locus_tag="ONT85_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2378575.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPQFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKTKETVKKDDFNEK"
     gene            complement(1222..1374)
                     /locus_tag="ONT85_001598"
                     /pseudo
     CDS             complement(1222..1374)
                     /locus_tag="ONT85_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
     gene            complement(1642..2973)
                     /locus_tag="ONT85_001599"
     CDS             complement(1642..2973)
                     /locus_tag="ONT85_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCR2378576.1"
                     /translation="MGKSSRKDIVFEKFGIVATKLGNQIHLRTLRDAFATFMPFMMLA
                     GFVTLINSVILEPTGFMGEIVDTSTLTTLQQIGTSIANGTLSITTLLVVAAVSYHMCA
                     SRNYTNHIAAVLVAISTFVVLTPMQMMFTPENADKAVEVTGVIPISYTGASGMFVGIF
                     VGLAATELFIKLSNNKKLQIQLSGNIPPAVLKSFNVLIPIIITITSFALLSFIIIQLF
                     NMDINKLITTIVTEPLSKITTGLPGFLLITSIANLFFGFGIHQAVISGSLLDPFLIQN
                     MQENMAAYANKEQIPHIINMAFKDTFAVMGGSGNTIALLIAIFIFSRRKDYKDFAKLS
                     VTPAIFNISEPIIFGLPIVFNISLIIPFVLAPIFSLTVAYFATAVGLINHVVVQIPWT
                     TPPVISGFLATAGDWRAAVLQILIIAASVFIYLPFLRIDEHVTTKLSENET"
     gene            complement(3122..3835)
                     /locus_tag="ONT85_001600"
     CDS             complement(3122..3835)
                     /locus_tag="ONT85_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016611336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR2378577.1"
                     /translation="MKKIAKYMEIYLDIKKKIVNGEYKIGEKLPTGDELAVIYHTSKL
                     TVKKGVDLLVSEGVLRSRSGYGTEVVRTPIDNSRVLGPNEGLFSVVGEEHVDSEIHTF
                     SIELPSKKVADKLSISVKDYVYNIVRSRFIDCQPYSIEQTFMPLAVIPGLEPKHLKKS
                     VYAYITNELGLEIKTSHIRIKGDTANEFDAKILKIDPGEFMIEIDKVVALSSGKPFEY
                     SVSRHLYQDFVFEAVFVEK"
     gene            4010..5434
                     /locus_tag="ONT85_001601"
     CDS             4010..5434
                     /locus_tag="ONT85_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HCR2378578.1"
                     /translation="MTTMKKLTFPDDFWWGSAWSAEQAEGKGETGKAETIWDRWFKEQ
                     PYRFYQRIDSSITTDHIHQYKEDIELMKKTGHNSFRVSISWARMFPNGGTGKVNPKAI
                     SFYRKLFSEMNNNGIKVFANLYHFDMPATLQDKGGWESREVVDAYVHYAETCFKEFGD
                     LVCHWFTFNEPLGPILGTYLEDFHYPNLIDFKRGAQAAFFTILAHAKAIQVFKELALP
                     SKIGVILNLSPTFPRSQNPADVKAAEIADLFYTRSFLDPMVKGNFSKKLILLLQEYNQ
                     LPEDYTKEDLVCIYENTAQILGLNYYEPRRVKSRSSMVDTSSPFLPEWFFEPHIMPGR
                     RMNEYRGWEIYEKGIYDLCMDIKNHYGNIEAFISENGMGVADEERFMDDTGQIIDDYR
                     IEYIKDHLAYLWKAINEGCNIKGYHLWTFIDCWSWINAYKNRYGLVSLDLTTQKRTIK
                     KSGEFYKQLSDTNGFRYDVDLLVE"
     gene            5733..6440
                     /locus_tag="ONT85_001602"
     CDS             5733..6440
                     /locus_tag="ONT85_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HCR2378579.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLIYAGSAQFITVSMLASHSPLLSIVLSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVILV
                     VTLIGCGLGVWIKHAFF"
     gene            6427..6750
                     /locus_tag="ONT85_001603"
     CDS             6427..6750
                     /locus_tag="ONT85_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HCR2378580.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRILPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLVGILSLS
                     LIRLL"
     gene            complement(6839..7876)
                     /locus_tag="ONT85_001604"
     CDS             complement(6839..7876)
                     /locus_tag="ONT85_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HCR2378581.1"
                     /translation="MSEKELMKVVGFYEGLSIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGAGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHIL
                     GKVVVSGPFNG"
     gene            complement(8197..9180)
                     /locus_tag="ONT85_001605"
     CDS             complement(8197..9180)
                     /locus_tag="ONT85_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HCR2378582.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKEPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILDTFEKEHPTLFSEQ"
     gene            complement(9595..10365)
                     /locus_tag="ONT85_001606"
     CDS             complement(9595..10365)
                     /locus_tag="ONT85_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR2378583.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(10358..11293)
                     /locus_tag="ONT85_001607"
     CDS             complement(10358..11293)
                     /locus_tag="ONT85_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR2378584.1"
                     /translation="MANALEINNLKKVYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDTDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYETKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(11609..13258)
                     /locus_tag="ONT85_001608"
     CDS             complement(11609..13258)
                     /locus_tag="ONT85_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HCR2378585.1"
                     /translation="MGQREFIVLIIIAAVILLVALDIFSRLKLKETVRSKWEKIPYQP
                     RFDKEESLKEAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDQQLEKLIAFYQENPQLREKIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGLGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKLPTPLQEELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAILNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLDYPETLVETAKTEAALFDEKRQWHVLG"
     gene            complement(13375..15978)
                     /locus_tag="ONT85_001609"
     CDS             complement(13375..15978)
                     /locus_tag="ONT85_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HCR2378586.1"
                     /translation="MVNAHIIQHTHWDREWYFTNEDAIVLSDQVFTEVLDELERNPKV
                     NFCLDGQTSIVDEYLEINPQKLKVIQRLMKTGQLFVGPWYAQTDALLVDAESILRNLM
                     IGISDTILKYGDPMMVGYLPDTFGFNANLPTLLHQVEIDNFMCWRGLNFETMVPSPYF
                     IWKGLGDKFVYAMNFPFGYMTGMMTLDAIKDIPEFAKEKLDEAVEMLHMQGKNKDILV
                     PSGVDQKSMILNFDQVVEKLNKYSKFHNIISDYPSYVEVIRNKKELPEYKGELRQPVY
                     SRVHRSIGSVRTKMKIENFKLEQKILKRVEPLMLIAERNGIHISKGLLRRLWKKILQN
                     QAHDSIGGCVSDNVAEDIFHRIKEANEIADGIENLIVKRMADALKLKSNEVLVFNTTT
                     ENYSGSKIVHVVADSKKIHFKGSPYNIIEKEVYFPERKNAYRMIATGFEYFDEPAYYE
                     LDIKINVNVPALGYTVVEFEKNNEKLETAIEIDKTEISNNKYKLSFKDGQLNLIVGDR
                     QYLDFVHLIDSANDGDTYDYSPLEGDTELSLKLETAKVYKDSLQETLVVYGKAQLPKD
                     LKDRLSEKPEMEEISYEISFSLGESQIVEGTLKIHNTVYSHRMRLAINVLDKSEKSIA
                     QIQNGFIENTPDVIPPNWSETMVEKPVNLEIFDKSVSVAENDHYFTFYADGLKEYERI
                     ENQLIITLFSTTGELGKPNLAWRPGRASGDTTNEGHVMMETPLAQEIGEYKVTFGFNV
                     EEGRFNEFKVAKQAEKRLEQSISYQKQKLNVFIHRLDNKIWPLQNELDVPRKFSLIEL
                     PEDLIVSSIYPSYFHEQGFVLRLANPTEQEKIVPEAILSLGTVVNALENKQELTTIPP
                     YDYLSILINER"
     gene            complement(15990..17039)
                     /locus_tag="ONT85_001610"
     CDS             complement(15990..17039)
                     /locus_tag="ONT85_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HCR2378587.1"
                     /translation="MEFKKQGREILNHFQSGVSYMIPLVVAAGLLTSIAVIFGGTGVW
                     DQTDTFWGVLRMIGQTGLQFIVPMISAYIAYSIADRPGLAPAFITGMMCQNLGMGFIG
                     GMVAGLACGYLANALKKVKVPMQVISLKSIMLIPFVTTLVVGIILWYVLATPIQAMTT
                     GLTNWLEGMSGANAALMSAILGGMMAFDMGGPINKIAYAFGVASFSSGSFGPSTAMLL
                     GIAIPPFGMFLATILNKKLYDESERDNAKTAMIMGLVGITEGTIPFAVKDPLRVIPSI
                     VVGTAVACGLNGFFGVTQETMLSTFMAIPFVTNIPLYLVSIVIGGVVTALMVNALKSI
                     KYKKEQQVNSVEEGK"
     gene            complement(17065..17379)
                     /locus_tag="ONT85_001611"
     CDS             complement(17065..17379)
                     /locus_tag="ONT85_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HCR2378588.1"
                     /translation="MTKIVAVTACIAGIAHTYMAKANLEKFAKKKGLDIKVETQGAMG
                     IENKLSISDVSEADVVIFAVDTNVSERGRFDGKPILTVGTSEVVKDGEETIDEAIALI
                     NK"
     gene            complement(17395..17841)
                     /locus_tag="ONT85_001612"
     CDS             complement(17395..17841)
                     /locus_tag="ONT85_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCR2378589.1"
                     /translation="MISEKININNIVIDLNASDKKSVVEKLSKLLETNGFVSDHKQFQ
                     KDVFDRENHMTTGIGKNIAIPHGKSLAVIESTVAVAKLKRSVEWGALDDQPVDLVFLL
                     AIKDTDKGTNHLRILAELSGRLMDDEFVKRIKDSRNKKELVKALSF"
     gene            complement(17842..19764)
                     /locus_tag="ONT85_001613"
     CDS             complement(17842..19764)
                     /locus_tag="ONT85_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HCR2378590.1"
                     /translation="MGIEFSERQMKILYFLIENEHTTYKELSEQLYVSTKTISKDILI
                     LKEIIQKMPDVQMLVQPKKGVSLIGKKETLRNLLSSHKINIADSPEDRLTYVFAKLLN
                     TNDSLKIQNLADELYVSRSTVESTMKDVRKRLNKHGMSLVSDRNGVRIKADEKSKRRL
                     MSEVVNYYWGGFTASNSKTDLLDLNINFIGEANNFLNKDILKKVAELLNRYMNKTQLK
                     FTDYEFQTLAIHLAIAIERIETSNFLSRSNNSEEEISKNTLILIKMIEKAFSIIVPID
                     EQAYVDIHITSIEKNAMNHDLDQTKLSYLDMGVELQELVRIKLNEFYPDEELINNLVL
                     HLNAAVKRLKLGVNIYNPYTDKIKYSFKRSFMISVDLLEEIEERFCIHFNEDEIAYVT
                     LHIQSLLDRYKPDKTKVILVCSSGYGTSKLLEQRINNGFADMVEIEDVLSINELQDRK
                     VTDELVISTIPIENTNFPVIVVSPLMLGADVRKVKQYLQLNEQTEDAFLNLLDKRFCF
                     FTHQIKDNQVNVLNIITKKLVKENYAKEGILEGAIKREKLSSTAMNDFAMPHIEVDFI
                     KKPVICVYVNPEGIDWDGTLVHSVFFFALNEKVKPELNKIYETFNEIASNHELLANLK
                     TSSSFEKLVYILKEGL"
     gene            complement(19976..20872)
                     /locus_tag="ONT85_001614"
     CDS             complement(19976..20872)
                     /locus_tag="ONT85_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HCR2378591.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWLDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDPFIRLTVANPIKPGTQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(20865..21545)
                     /locus_tag="ONT85_001615"
     CDS             complement(20865..21545)
                     /locus_tag="ONT85_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR2378592.1"
                     /translation="MVLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(21546..22328)
                     /locus_tag="ONT85_001616"
     CDS             complement(21546..22328)
                     /locus_tag="ONT85_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR2378593.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMLAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LTLGTLSVPVALTFITRSTSVSLLLSFLYIMGTAFIPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(22321..23238)
                     /locus_tag="ONT85_001617"
     CDS             complement(22321..23238)
                     /locus_tag="ONT85_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR2378594.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(23402..23725)
                     /locus_tag="ONT85_001618"
     CDS             complement(23402..23725)
                     /locus_tag="ONT85_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2378595.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWKTTQPRLRDSLIFLGGSLAIVILITGLVKLLKLDLKEAKIFSEEEYRETTLVEK
                     KESGN"
     gene            24513..27740
                     /gene="fss3"
                     /locus_tag="ONT85_001619"
     CDS             24513..27740
                     /gene="fss3"
                     /locus_tag="ONT85_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HCR2378596.1"
                     /translation="MYKKQKKKLCVLTILSLAVQAIVGAIAPTITFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGFKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIDDYQKKPTFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENAANIDYRVN
                     GNQLIITPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKESGDVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTTTIKAGETIYVTSKNAREKGQIILDKSGVETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDENGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELKYANQTVAPVTSNVKGQNQEVTGETTLTKEDKDTGDKAQGKAVFEGAEYTL
                     FTAKDGKAVKWSEAFKPELAKGTKGSDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYSLDETKYPVSIKKVDDSEKNAVITRNVTAKEQVIRFGFDFFKFAGSAAGTTETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDFVKSEYVFTITEQDQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLATWKDGNKELTSLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     DATKAAQEKDEKAKPVVIAETSATLANKEKTGTWKIQHKLTAEQVLNKTIVLFNYVYE
                     NKEAFEAGDKPVAKDVSLNNQAQTVSCTVEHHVSIQTKAHLENGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDGTNKEIWKSGKIDYEVNDKEFTKTVLAKKVDT
                     GKYPEGTKFTFAEINYDKDGNINGKHNEDLKEKSQTLTPKEVPTILSTPKQPETPTVP
                     SGSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            27853..28167
                     /locus_tag="ONT85_001620"
     CDS             27853..28167
                     /locus_tag="ONT85_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HCR2378597.1"
                     /translation="MELKFVVPDMAETFGKLSYAGEGEIITEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            28180..28554
                     /locus_tag="ONT85_001621"
     CDS             28180..28554
                     /locus_tag="ONT85_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HCR2378598.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTIGNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPNQGNPTNQNERKK"
     gene            28564..28899
                     /locus_tag="ONT85_001622"
     CDS             28564..28899
                     /locus_tag="ONT85_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HCR2378599.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLVKALTSDEKMFLIIHQGLL
                     GENSDSEEDSDVLDWLGEEESFFIVVEVNENLCRQAESILEEIGVEMPDAIEGFLNQL
                     VETKQLPSS"
     gene            28982..30322
                     /locus_tag="ONT85_001623"
     CDS             28982..30322
                     /locus_tag="ONT85_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HCR2378600.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQEFRQFDI
                     SKLSLSTIEIVWSIKDLVCLLGSLAFSGAMILLYIHFFLDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTRETISYFPKICYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIDEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMARSKAMKEMSNYKTGENYAYLGLPPNFLIFDEYVAYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVEKNFFMKHIKGRGYVDTGGSVISEFYTPLVPKGYDFLDSISKLEKMND"
     gene            30400..31074
                     /locus_tag="ONT85_001624"
     CDS             30400..31074
                     /locus_tag="ONT85_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HCR2378601.1"
                     /translation="MESNDSYLEYAKANKDKFISYIIKGKSPEVGRDAVFMAGSPGAG
                     KSEVAIGLAENYDNHVIIDADYFRTQFPDYNGKNSSLFQKASSWLVEQAFRYVLDHGY
                     SFILDATFAILSAEKNISRALKKHFRVTIFYVYQDPKIAWKFTQEREIAEGRHVPKKA
                     FINAFFKSRENIAKVKSKHPDVLLHIIIKDYTNNISEVHYATDNLELFLPLGYSRKDL
                     EVQLHD"
     gene            31067..31231
                     /locus_tag="ONT85_001625"
     CDS             31067..31231
                     /locus_tag="ONT85_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2378602.1"
                     /translation="MTEKEQVTKIVKKYNKSIADLSENATAKEFKTVIKYVADQANEK
                     QRKLVGLNKK"
BASE COUNT         9736 a         6069 c         5515 g         9925 t
ORIGIN      
        1 ataaataaaa aacaaaaaaa gttccatctt taaaacacgc tattctttta ctttttctca
       61 cattctctaa gaaaagaaaa ggagaatgaa tttttgacaa ttcgagcaaa agtgattatc
      121 aaatatgtaa aatcagctac ttttttctta aacgtttctt tatactaaat ataaataagt
      181 gtttcatgaa aggagaacta gatgaataat tggcttgagt attttcctga aaacgttttg
      241 gaacgcggtt acagctatca tctgcacggt tttgtccgtc atttgaacta tacaagcaag
      301 tacttgtccg caacagtttc tggcacggaa gattataagg tagtgatcac ttgggatgaa
      361 aagaccaaca tgacttgtga ttgtctatat gccatagaag gaaaaaaatg caaacacatg
      421 gctgctgtct tattcgctta cgaagaacgt ccaataaaaa aatccaacta ctcactttcc
      481 gaactctcgt cccttgtttc ctccgctagt tcctctcttg tacgggagct tttaacagag
      541 attcttatag aacatccgca atttatcgaa cgcttcaaag ttaagatgcc atttcatgca
      601 ataaattatt cagataaatt aacaactatc atccataaat acgatcatat tatcaagaaa
      661 aacaaaaaca ggaaaacagc gaagttcatc atggagatgc gaaagttcat ccaagaagca
      721 gttgaatcgc tcattcagca aaatgcctat cttcccgcat tcgaattgat caatgaagtg
      781 attgctactc tcgaaacatt ctattgggag cctgaggatg aacgaaccct tcttctgata
      841 gaggactgtt attatttatg gaaagaatta ttagcagaag cacctcatgc agaaaaaagg
      901 caaatgttct cttggttcgt ctgccaagtc gatcatactg acgcctctta ttcaaaacgc
      961 tactccataa aaatcctgaa agaagacttt cgggaaaaag aattttctaa ccaaaagaaa
     1021 aaaatagata aaaaaactaa agaaacggta aagaaagacg atttcaatga aaaatagcaa
     1081 gtaaacgata acaaccatcc taaatccaca agaaatacag cagaccttgc tttaaaaaca
     1141 cgaatcatgc aaaggatgaa gtaaagaaga cttaacgcgt acccaatatt agtcatttga
     1201 ctattaggta cgcgttttgt tttataagtc atgacaaatt tccttcgttg ctgggtagat
     1261 tttccggcat tgttgataga ttttatgata cttttctaca ttttgtaaga gaggttccac
     1321 tgcatctgta tattggataa aattctgaca acaactttca ggtgtgtcga accactctaa
     1381 tctttaaaga taaatattaa aataacgatt aataatatta acagtcttta tttttcacca
     1441 ccaaaaacct atctttgcat atactaaata gacgtatata cacagatagg tttgtcttgt
     1501 gaaaaataat gattatcctt accaaaaaat gtttgttttt ctacatgttc gtacttatca
     1561 aaaaatatga tccttattga aattactggc tatttatatt ccaacgactt cttgtgttaa
     1621 tttttgttat ctcatagtaa attatgtttc attttcactt aatttagttg tcacgtgttc
     1681 gtcaattcgt aaaaacggta aatatataaa cacacttgcg gctataatta atatttgtaa
     1741 aacagctgct cgccagtctc cggctgttgc taaaaatccc gatataactg gtggcgtagt
     1801 ccaaggaatt tgcactacta catgattaat taatcccaca gcagttgcaa aatatgctac
     1861 tgtcaaagaa aaaatcggtg caagcacaaa gggaataatc agactgatat taaatacaat
     1921 aggcaatcca aaaataatag gttcgcttat attgaatata gcgggagtta ccgataattt
     1981 agcaaagtcc ttataatctt ttctacgact gaagataaat attgcaatca ataaagcaat
     2041 cgtatttccc gatccaccca ttaccgcaaa ggtatcttta aaggccatat tgataatatg
     2101 tggaatttgc tctttgtttg cataagctgc catattttct tgcatattct gaatcaaaaa
     2161 cggatctaac aatgaacctg atattactgc ttgatggata ccaaatccaa aaaatagatt
     2221 agcaatagat gtaattaaca aaaatcctgg taaaccagtt gtaattttac ttaatggctc
     2281 tgtaactatc gtggtaatta gcttatttat atccatatta aacaattgaa tgattataaa
     2341 tgacaataaa gcaaacgagg taatcgtaat aatgattggg attaatacat taaaagactt
     2401 taaaacagcg ggaggaatat ttccggatag ctgaatctgt agtttcttat tattagaaag
     2461 tttaataaat aattctgtag ccgctaatcc aacaaatata cctacaaaca ttcctgaagc
     2521 tcctgtatag gatataggta tcacacccgt cacttcaaca gctttatccg cattttcagg
     2581 agtaaacatc atctgcatag gtgttaatac aacaaatgtc gaaatagcaa ctaataccgc
     2641 tgcaatatga tttgtataat ttctgcttgc acacatatga taagatactg ctgctacaac
     2701 taataatgta gttatgctta atgtaccatt ggcaattgaa gtaccaattt gttgtaacgt
     2761 ggtcaatgta ctagtatcga caatttcccc catgaatcct gttggttcta agatcactga
     2821 attaataagt gtcacaaatc ctgctaacat cataaatggc ataaatgtag caaacgcatc
     2881 tcgcaaagtt cttaaatgga tctgattccc caacttagtc gcaactatac caaatttttc
     2941 aaatacaatg tccttcctgc tacttttgcc catatttttt ctcctttatg cttattgtaa
     3001 tcgattactt tttccaccca aataatacat cacttatcaa aaaaagtaaa tacttttaat
     3061 taaatatata tatctttttt agtataaaaa aataagctct gaaaaaataa ttttcccgag
     3121 cttatttttc aacaaaaaca gcttcaaaaa caaagtcttg atataaatgt ctcgataccg
     3181 aatactcgaa aggtttacca gacgatagag caacaacttt gtctatctca atcataaatt
     3241 cacctggatc tatttttaat atttttgcgt caaattcatt cgcagtatct cctttgatac
     3301 gtatgtgcga agtctttatt tccagaccca gttcatttgt gatataagca tatacagatt
     3361 tctttaaatg ttttggttcc aagcctggta tgactgctaa tggcatgaat gtttgctcaa
     3421 tcgagtatgg ttgacaatca ataaaacggc ttcgtacaat attatataca tagtctttta
     3481 cactgatact taatttatca gctacttttt tcgatggaag ttcaattgag aaagtatgga
     3541 tttctgaatc tacgtgttct tcaccaacca cgctaaacaa accttcgttt ggaccaagaa
     3601 ctcgtgagtt atcaattggt gttctgacga cttcagtccc atacccactt ctacttcgca
     3661 aaactccttc ggaaacaagt aagtctacgc ctttctttac tgttaacttg cttgtatgat
     3721 aaataactgc caattcatct cctgtaggaa gtttctcacc aattttatac tctccattta
     3781 caattttttt ctttatatct aagtaaattt ccatatactt cgcaattttt ttcaatggtg
     3841 accctccaat caaaaacata tcttcatcta tactttaaca taaataaacc gataaaatcc
     3901 acatcaaaat tatacacaca ataaaaaaga tatatatatt taaacaaaac tatttacttt
     3961 aagttgtaag cggtgttatc attaagtgga aacaggaggt aaaaaaatta tgacaactat
     4021 gaaaaagcta acttttccag atgatttttg gtggggttcc gcttggtcag ctgagcaagc
     4081 agaaggaaaa ggagaaacag gaaaagcaga gactatctgg gatcgctggt ttaaagaaca
     4141 accttaccgg ttttatcaac gaatcgattc ctctattaca accgatcaca tccatcaata
     4201 taaagaagat atagaactaa tgaaaaaaac tgggcataac tcctttcgcg tttctatttc
     4261 ttgggcacga atgtttccaa atggcggtac cggaaaagta aatcccaaag ccatttcttt
     4321 ctatcgaaaa ttattttccg aaatgaataa taacggaatc aaagtatttg ccaacttata
     4381 tcattttgac atgcctgcta ctcttcaaga taaaggagga tgggaatcac gtgaagtagt
     4441 tgatgcttat gttcattacg ctgaaacttg ttttaaagaa tttggtgact tagtttgtca
     4501 ttggtttacc ttcaatgaac cacttgggcc tattttaggt acttatcttg aagattttca
     4561 ttatccaaac ctcattgact ttaaacgcgg agcgcaagca gcattcttca ctatacttgc
     4621 acacgcaaaa gcaatacaag tatttaaaga actagctctt cctagtaaaa taggcgttat
     4681 tctaaatttg agtcctactt ttcctcgtag tcaaaatcct gctgatgtga aagcagcaga
     4741 aatcgctgat ttattttata cgcggagctt tttagatcct atggttaaag ggaatttttc
     4801 taaaaagctt atcctgttat tacaagaata taatcagctt cctgaagatt atacgaaaga
     4861 agatcttgta tgtatctacg aaaataccgc tcaaattctt ggactaaact attacgagcc
     4921 acgtcgcgtc aaatctcgtt cttctatggt ggatacatct agtccatttt taccagagtg
     4981 gtttttcgaa ccacatatca tgcctggacg acgaatgaac gaataccggg gatgggaaat
     5041 ttacgaaaaa ggaatttatg atttgtgcat ggatatcaag aaccattatg gaaacatcga
     5101 agcatttatc tcggagaacg gaatgggcgt tgcggatgaa gaacgtttca tggatgatac
     5161 agggcaaata attgatgatt atcggattga gtatatcaaa gatcatcttg cttacttatg
     5221 gaaggcaata aatgaaggat gtaacattaa ggggtatcat ctttggacat ttattgattg
     5281 ctggtcatgg atcaacgcct ataaaaaccg atatgggctg gtttcactcg accttactac
     5341 tcaaaaaaga acaattaaaa aaagtggcga attttacaag caattaagtg atacaaatgg
     5401 atttagatac gatgtagact tgttagttga ataatattct aaaattccac ttgaagtgaa
     5461 cacattagaa aacaagaata atgagaaata ccaataacaa atactttttc ttcatacttc
     5521 atttatactt gtcagtataa gagactgtga caaatgtagt gatcctcaga agttaggtct
     5581 ggaaatctaa cttgagaaga tttttttaaa ctacgtccag tcttttttat ttgtttaaaa
     5641 ttgaagttga caagtcttca ttaaaaattt agaatattca caagtttgac gacaattatg
     5701 aaaatttatg tcgtgctaaa ggagttttgt tattggatga aaagttagat ataaaaactg
     5761 cgatcaaaga cacgttacct accgtttttg gttatatcgg tattggactt gcatttggta
     5821 tcgttgggaa agctgccggg tttcatccgc tggtcgtcac gttgatgtcc ctgctgatct
     5881 atgctggttc tgcccaattt atcacggtca gcatgcttgc tagccatagt ccactgctct
     5941 ccatcgttct ctcgaccttt ctagtcaatt cccgaatgat tctgatgagt atgacgattg
     6001 ctccttattt caagaaaaat cggctgcttc aaaatctgct gattggcaca ttgctgacag
     6061 atgaaagttt cgcactggga atgaataaat tgaactatac tggacaaaag ttaaactttc
     6121 gttggatgaa tacagctaat tggatttctt atttgacgtg ggtgggttct tctcttgttg
     6181 gcgcactttt agggaatttt attactgatc cgaaaaagtt tggtttggat ttcgccatcg
     6241 tcgctatgtt tatcgggtta ctttatcttc aagtcatttc tgatagaaac acctctaaac
     6301 gattacagtt gatcctgatt ggtctcactt tgattctggt atatgtcgga ctgattttta
     6361 tcccaagtaa cttagtgatt ttagttgtaa cactgattgg gtgcggattg ggggtgtgga
     6421 tcaaacatgc cttcttttga gtttacattg ctgactattt taggttgtac tttagcaact
     6481 tggctttcta ggatcctacc atttgtctta ctgaaaaaat tcgatttgcc gcaaccgttg
     6541 cttgagtatt taagttttgt cccgatcgtc attatgtctg ctttatggtt cagcagtcta
     6601 tttacccaaa atatcggaca tctgccgcag atcaatctcg aaaatgcttt ggcttcagtt
     6661 cctacacttt tagctgctat tctttcaaaa agcttattgg tgatcgtctt ggtaggaatt
     6721 ctttcccttt cgttgattcg cttattataa ataaaaaaac aagagcttat ggcaaattac
     6781 tgtcatgcgc tcttgttttt atcaaagtca aataagaaaa aaatcggcgt attcttgctt
     6841 atccgttgaa cggaccacta acaacgactt ttcctaatat atgtcctgtt tctacgtctt
     6901 tggttgcttg tttcaagttc ttcgcattca tcccatctga atagaccttg ctcaatgtac
     6961 tgcggataat cccttcattt gctaaagcag caatgtgcgc taaagcttct ccttgcgttt
     7021 cgatttcata gttatagtct gttttagcga acatatactc ccagtcgaag ctgatagatt
     7081 ggtttttcca aacacctata tctaacggct ctttgatccc tacgatcgtt cccacatgtc
     7141 caaatggtcg gacaaggtgt ttgatttgat caaagtagcg ggtaatatcg aataatacag
     7201 cgacatactc tacttgatcg attcctaatt cacgaaaact tttacttaag tcgtgatgat
     7261 aatcgattgg atagtctaca cctgtttttt tcagccattc aaaattcttc ggacttgcag
     7321 tcgcgtagac ttctaggcca gcccaatgcg ctagttgatt caaaatcgaa ccgacgcccc
     7381 cagctccgtt gatcactaag atttttttcc cgcggttggc attttcttct ggagtcagac
     7441 caaatttttc aaataacaat tcataagcgg tcaatgaggt caacggcaag gctgcggctt
     7501 cttcatctga aagatttttt ggtgcaagag cgacaatacg ttcgtcaacc agttggtatt
     7561 cttgattgct tcctgctctt gtcgttgtac ctgcgtaaaa aacgcgatcg ccgacagaaa
     7621 atttttgcac ttcatcacct acagcaacaa ctttcccgac tccatcaaac cctaagatac
     7681 gtaaagcatt tcttatcccg ttatcttggc gaagtttcgt atcgacagga ttaacagata
     7741 ctgcgttgac ttttaccaga agatcccgtg aactgggtac aggtatctgt tctttttcat
     7801 caataaagct ttttggatca tcgatcgaca acccttcata aaaaccgact accttcatta
     7861 attccttttc tgacatacga attcctcctt agcttcatca agttggagca tccatacagt
     7921 tttcatttca atatatatag tatgctattt gttccacttt tattcatcat aattattctc
     7981 ttacttaaag taaactattt gcactttttt ggttaaaaaa gaggttgcga caagactttt
     8041 gtcacaacct cttttaaacg gtgttcaatc aactgaccct cggtattcgc tcaaaaaacg
     8101 acaaaatatg aggagctatt tttgtttggc tgttcttatt acttgatgca ggacagcttt
     8161 ttcacgacct ctaattcacg gtgttcaagc aagaaatcat tgttcactga aaagcgttgg
     8221 atgttctttt tcaaaggtgt ctaagatctc tcgtacttca tcagtagatt tcgtattcat
     8281 caactgaact cgcaagtcat ttgctcctgg aaatcctttg atatagattt taaagaaacg
     8341 atgtagtcct gtgatggaac gcgggatgat ttctgaataa tgatcttgca gatctagttg
     8401 cagacgaagt agtccgatca gttctttttc tgtatgttcc ctcggttctt tttcaaatgc
     8461 ataaggattc ttgaaaatcc cccggccaat catgatgcca tctacgccat atttttcggc
     8521 caattcttct cctgtctggc gatctgggat atctccgttg atcgtgatca gtgtttgcgg
     8581 agcgatttgg tcacgtaaag caatgatttt agggatgact tcccaatgtg catctacttt
     8641 actcatctct ttacgcgtac gcaaatggac agaaagattg gctatatctt gatgaagtag
     8701 gtgactgatc cattcttcca tctcagacat ttccgtatag ccgatacgcg tcttgacgct
     8761 gacaggcaaa ccgccagctt tagccgcttc aatcaattgt gccgctactt ctgggcgaag
     8821 gatcaagcca ctgccttttc cacgttctgc tacattaggg actgggcagc ccatgttgat
     8881 atcgattcct tggaagccca tttctgccat agcaatgctc atttctctaa aaaattctgg
     8941 cttgtctccc caaatatggg caaccatcgg ctgttcatct tctgtaaagg tcaatcttcc
     9001 tcgtacactg tcttttccat ctggatgaca gaaactgtct gagtttgtaa actcagtaaa
     9061 gaagacatcc ggtgctgctg catgctttac gacatggcga aatacaacat ctgttacatc
     9121 ttccattggt gctaaaacaa aaaaaggctt tggcagctct ttccaaaaat tagtagtcat
     9181 ttatttgtta ctctccattc tatcaagcag tgaagtcgca atctttctca aaaacattgt
     9241 cattatacta ggaaacaaaa aaagaagcaa agtgaacagc ttaaaagatg tttctgattg
     9301 agcaaaaaaa gactgtgaca aaaatcttat cacaattcct tttccggaat aaatggtgtt
     9361 caaaagcttg catctgcggt aataagtcca accaaacgaa aaataggagg agctattttt
     9421 gtttggctgt tcttattact tgatgcagaa cagctttttc acaacctctc cactaaacgg
     9481 tgttcaatca gctgaccctc ggtattagct caacaaacga caaaatatga ggaactattt
     9541 tcccgtttgt ttctctaata ctcaggtcaa aacgctgatt tcacaacctc ttttctaact
     9601 tctcaatcct cttcctttat tgatcaaata ccaagtaacg gcgtacaaaa caacgatgaa
     9661 tagcacaagt acgatcatcg atgttgctac tggcacatcg attgttccaa ggaatccgta
     9721 acggaaaccg gagatcatat aaacgatcgg attgactttc gaaacagctt gccaaaatgg
     9781 cggcaacata gagatggcat agaaaacgcc acctagataa gttaacggct gcaagacaaa
     9841 tgtaggcacg atcgatacat cgtcataaga ctgagcaaaa atcccgttga gcagtcctgc
     9901 taacgaaaac aagattgccg tcatcaataa tgtaatgatg accatgaacc atgaatacac
     9961 atgaagtggt acaaagaaca aggaaatgat cgttacgaga gagccgacga gaatactgcg
    10021 acccactccg ccaatcacaa agccccaaat gatcacatgc gtaggaacag gcgctaccaa
    10081 gatttcttcg atgtttttct ggaatttttg agagaagaag gacgaagaaa cgttggcata
    10141 cgaactagtg atagcagaca tcataatcaa accaggcacg atgaattcca tatatgaaaa
    10201 accacccatg tcaccgatcc gaccaccgat catttttccg aaaataacaa agtacaagga
    10261 tgtcgtgatc acaggcggaa ccagtgtttg gacccagata cgcaaatagc ggttcgtttc
    10321 cttgatagct aaacttttta atgcagtaaa ataaagatta aacatttttc tcctccgtat
    10381 gattattttc ttctgtgatt ttcaaaaaca gttcttctaa acgattcgac ttattcctca
    10441 tagaaagaac tttgattcct tgtcttgtca gctgatcgaa gattccgttc accccttgat
    10501 ttcgttcaac ttcgacagcg attgtctgtt catcttcaaa tgtacaagcg tatccttcaa
    10561 tcacaggttt ggtctcatat ggcgctaaat caaagataaa tgtttcaaat tgcagtttgg
    10621 atagaagctc tttcatacta gtattctcga tcagttcacc tgattggatg atcccgatgt
    10681 tgcgacaaag catttcagct tcttctaaat aatgcgtcgt caaaatgatc gttgtaccat
    10741 tttcgttcaa ttctcggaga aactcccaca tctcgcgtct caattcaata tcgactcctg
    10801 ctgttggttc atccaagatc agcaaacgtg gttcatgcat caatgcccta gcaatcatca
    10861 gtcttcgctt cattcccccg gaaagcatac gtgcacggac atttctcttt tcccaaagat
    10921 tggactgctt caaatatttt tcgctgcgtt tcatcgcttc tttgcgagag acaccgtagt
    10981 agcctgcttg attcacgacg atttgctgga ccgtttcaaa gggattgaaa ttgaactctt
    11041 gcgggactaa tccaatctgt tgctttgctc ggactagatc cgtatccaga tcataaccga
    11101 agacattcac ttttccagat gttttattga ctaaagaagt aatgattccg atcgttgtag
    11161 acttcccagc tccatttggt cctaatagcg catagaaatc tccttctttc acggaaagat
    11221 cgattcctcg caatgcttcg accccacttc cataaacctt tttcaaatta tttatttcaa
    11281 gcgcatttgc catttatttc ctatctcctt atagacatga ttctttattg tatcatggaa
    11341 tacagcagat gaaaaaacaa cttggttcca gcgaaaaatc aggttgctgg gctcaggaaa
    11401 atctcctaat gaaagacatc aatcggacga agtcagacaa ccttttcaga aaaaacaaat
    11461 cgaagataaa ggattactat tcatatgtgc aacaaaagga gcgtgagaca aaagtaaaga
    11521 acacttttgt ctcacgctct aaacacgtat aaacggcgga agcaaaagcc aagatttttg
    11581 atccaataaa tactttcgat cacttcgcct atcccaatac atgccactgc cgcttttcat
    11641 caaataacgc ggcttccgtt tttgcggttt ctactaaagt ctctgggtaa tccaaaactt
    11701 tcagcaaggc aatcgcattt cgggtccttg aaggtccttc ttttaatttg aaatcaaaac
    11761 tgattccttt tccttctgtt acttgttctt caaagtggac attctcacaa taattcttta
    11821 aaatctctgt caattcgata tcatgtgtgg cgacaaatgc taaagatgga tactccgcaa
    11881 gccaccgaac gactgaagcg gaagaagcaa tccgttcgat cgtatttgtc ccttttaaaa
    11941 tctcatctac gaagcagtag cagcgttctt tggaagccac ttggtccaac aaacgtttga
    12001 tcgatttgat ttctgaaaca aaataactgt cgccttcaaa taaatcatct tcgattgcca
    12061 tggaagtcaa cacatgtcca ggttgcaggg taaactgttc tgcaatcgct gtttggatcg
    12121 tctgtgctag aatacagctg atggctatac ttttgacata cgtagatttt cctgatgcat
    12181 tggaacccgt gactaatgta ttcttctgcc agttgaccgg attcactacc gcttctttta
    12241 acaatggatg atagatatct ttcgctgtca caccgccctc agtaaactct ggtcggcaag
    12301 tgatgggcat ataagtacgg taattcaata ttgcagcggc tacttctaat tctcccagca
    12361 attcccacag cttgatggct tcagttgaat gcttttctaa tttagtgaga acgaaatgat
    12421 aggagataaa tggcagcatg aacatgatgt tgacatattc aaaaatcaac tctgcttcac
    12481 tcccactctt catacgaaat gaaaaaccta atttagggat gtctcttaat ggtttagttt
    12541 gctgtttcag ctcttcttga agtggtgtcg gcagttttgc tagctgattg gcggctgaaa
    12601 tcgttgaaac caaataactc atgctattca gctctgtttc tagtgtttgt ttcttcacta
    12661 gataatagac cgtattgaat actaacccac cgattgccaa atagattcct agtacttgtc
    12721 ctaaaagaag caaacccaaa cccaccagcg gaagcaagcc aagaaaaata aataaaccta
    12781 cgcgtccaat ctttttactt tttccatccg ctagatattg ttttgcaaaa ttatgatctt
    12841 tttttcctaa acgagcgaat tgatattgga ttttttctct cagttgagga ttttcctgat
    12901 aaaaagcaat cagtttttct aactgctgat cttcaccaaa atcaaagctg cgtaaacgtt
    12961 gatacaacgc ttctgaacct acactagaat aggtactgtt gattccttcg aatacttcga
    13021 acatatccag gtcataccaa gtaagatcat cgatttcgct atcccatgaa cggaacttct
    13081 tctccgtgag ccatgcttcc ttcaagcttt cttctttatc aaatctcggt tgataaggaa
    13141 ttttttccca tttgctacga actgtttcct ttaatttcaa acgtgaaaaa atgtctaaag
    13201 caactaataa aataacagct gcaataataa ttaaaacgat aaattctctt tgacccatat
    13261 ccctctccgt tcctttattt gttagcttta gtatagcact gttgcgctat ttgaaaagag
    13321 ggtaaaaaaa cacctttcaa aataaaaggt gtttttttct tgtatattac atatttatct
    13381 ctcatttatc aaaattgaca aataatcata gggaggaatt gttgttaatt cttgtttatt
    13441 ctccagtgca ttgacaactg tcccaaggga aaggatagcc tcaggtacta ttttttcttg
    13501 ttctgttgga ttagctaagc gaagaacaaa tccttgttca tgaaaataag aaggataaat
    13561 cgatgataca attaagtctt caggtaattc aatcaatgaa aatttacgag gaacatcaag
    13621 ttcattttgt aatggccaaa tcttgttatc taacctgtga ataaatacat tcagtttctg
    13681 cttttgataa gaaatagatt gttctaaccg tttttctgcc tgttttgcca ctttgaactc
    13741 attaaatctt ccttcttcta cattaaatcc aaaagtgact ttatactcac ctatctcttg
    13801 agctagcggt gtttccatca tgacatgccc ttcatttgtc gtatctcctg aagctcgccc
    13861 aggtcgccaa gcaagattag gttttccaag ttcccctgtt gttgaaaata aggttatgat
    13921 taattgattt tctatacgtt catactcttt taaaccatct gcataaaacg taaaataatg
    13981 atcattttct gccacagaga cactcttatc gaatatttct aaattaacag gtttctctac
    14041 catagtttct gaccaattcg gaggaatgac atcaggggta ttttcaataa atccgttttg
    14101 aatttgtgca attgattttt cagatttatc taaaacatta attgctaagc gcatacgatg
    14161 cgagtaaaca gtattatgaa ttttgagtgt gccttcaacg atttgacttt ctcccagact
    14221 gaacgatatt tcataagata tttcttccat ttcgggtttt tcagataaac gatcttttaa
    14281 atctttagga agttgagctt ttccgtaaac aactaatgtt tcttgtaaag aatctttata
    14341 tactttagct gtttctaatt ttagagatag ttctgtatct ccttctaatg gtgaataatc
    14401 atacgtatcg ccatcatttg ctgaatcgat caaatgaacg aaatcaagat attgtctatc
    14461 tccaactatc aaattcaatt gcccatcttt aaaagaaagt ttatatttat tgttacttat
    14521 ctcagttttg tctatctcga ttgctgtttc taatttttca ttatttttct caaattcaac
    14581 aacagtgtat cctagtgctg gcacatttac atttattttt atgtcaagtt cataataagc
    14641 tggttcatca aaatattcga aacctgtagc aatcatccga taagcatttt tccgttctgg
    14701 aaaataaact tctttttcaa tgatattata gggactacct ttgaagtgta ttttctttga
    14761 atcagctact acatgcacga ttttcgatcc actataattc tcagtcgttg tgttgaaaac
    14821 aaggacttca ttcgatttta gctttaatgc atcagccatc cgttttacaa taaggttttc
    14881 gattccgtca gctatctcat tagcttcttt gattcgatgg aaaatatctt ccgcaacatt
    14941 atctgaaaca cagccaccaa tactatcatg agcctggttt tgcaaaatct ttttccataa
    15001 ccgacgaagt aggcctttac tgatatggat accatttctt tctgcaatca gcatgagcgg
    15061 ctcaactcgt tttagaattt tttgttccaa tttaaaattt tctattttca tttttgttct
    15121 tactgagcca atagaacgat gaacacgaga gtaaaccggc tgacgaagtt cacctttata
    15181 ttcaggtaat tcttttttgt ttctgataac ttctacataa gaaggataat ctgaaataat
    15241 attgtggaat ttactgtatt tattcaactt ctctacgact tgatcaaaat ttaaaatcat
    15301 tgacttttga tctacacccg aaggtactaa aatatctttg ttttttcctt gcatatgaag
    15361 catttcaact gcttcatcca acttttcttt tgcaaattca ggaatatctt taattgcgtc
    15421 taaggtcatc attccagtca tataaccaaa tggaaaattc atggcataaa caaatttatc
    15481 tcccaatcct ttccagataa aataaggaga tggaaccatc gtttcaaagt ttaatcctcg
    15541 ccaacacatg aaattatcga tttctacttg atgcaaaagt gtaggcaaat tcgcattgaa
    15601 cccaaacgtg tcaggcaaat accctaccat cattggatct ccatatttaa ggatcgtatc
    15661 actgattcca atcattaagt ttcttaaaat tgattcagca tccactaata aggcatctgt
    15721 ttgtgcatac cacggtccca cgaatagttg tcctgttttc atcagtcgtt ggataacctt
    15781 aagcttttgc ggattgatct ccaaatactc atcgacgatt gaagtttgtc catctaaaca
    15841 aaagtttact ttagggttcc tttccaattc atctagaact tcagtaaaga cctgatcaga
    15901 caatacgatg gcatcttcat ttgtaaaata ccattctctg tcccagtgcg tgtgttgaat
    15961 tatatgtgca tttaccatat cagattctcc tactttcctt cttctactga atttacttgt
    16021 tgttcttttt tatacttgat ggattttagt gcattgacca ttaacgctgt aaccactccc
    16081 ccaataacaa ttgatactaa atataaaggt atattcgtaa caaaaggaat tgccataaag
    16141 gttgaaagca ttgtttcttg agtaacacca aaaaatccat ttagaccaca tgctactgca
    16201 gtgcccacca cgatgcttgg tattactcgt aaaggatctt tcacggcaaa cgggatcgtc
    16261 ccttcagtaa tccccacaag gcccataatc atagctgtct ttgcattatc tcgttccgat
    16321 tcatcataaa gttttttgtt taaaattgtt gctaaaaaca ttccaaatgg cggaatagca
    16381 attcctaata acatggctgt actaggtcca aagcttccgc ttgaaaaaga agcaacccca
    16441 aaagcatatg cgattttatt gataggtcct cccatatcaa acgccatcat tcctcctaaa
    16501 atagccgaca tcaaagcagc atttgctcct gacatgcctt ctagccaatt agtcagtcct
    16561 gttgtcattg cttgaatagg cgtagctagt acataccata atatgattcc aacaactaat
    16621 gtagtaacaa aaggaatcag cataattgat tttagactaa taacttgcat cggtactttt
    16681 acttttttca atgcattagc taaatagcca caagctaatc cagcaaccat tcctccaata
    16741 aatcccatac caagattttg acacatcatc ccagtgataa aagctggtgc tagaccggga
    16801 cggtcagcaa ttgaatatgc gatataggct gaaatcatcg gtacgataaa ctgtaatccc
    16861 gtctgaccaa tcattcgcag gactccccag aatgtatccg tctggtccca aacacctgtg
    16921 ccaccaaaaa tgactgcaat agaagttagt aaccctgctg ctacgacaag cgggatcata
    16981 taggaaaccc cactttggaa atgatttaga atttctcttc cttgtttctt gaattccatt
    17041 agtaactccc ccacgatata ttttttattt gttaattaat gcaattgctt catcaatcgt
    17101 ctcttcacca tcttttacta cctctgaggt ccctactgtt aaaatcggtt taccgtcaaa
    17161 gcgacctcgt tctgaaacat ttgtatctac tgcaaatatg acaacatctg cctcggaaac
    17221 atccgaaatt gaaagtttgt tttctatccc catagccccc tgtgtttcta ctttaatatc
    17281 taaccctttc ttcttcgcaa acttttctaa attagctttt gccatataag tatgggcaat
    17341 ccctgcgata catgctgtta ctgctactat ttttgtcata tgaaattcct ccttttaaaa
    17401 tgatagagct tttaccaatt cttttttatt tctcgaatct tttattcttt taacaaactc
    17461 gtcatccatc aatcttccag ataattctgc taaaatacgt aaatgattag tacctttatc
    17521 agtgtctttt attgctaaca aaaagacgag atcaactggt tgatcatcta atgcgcccca
    17581 ttcaacagac cgctttaact tggctacagc cactgttgat tcaattactg ctaggctttt
    17641 gccatgagga atggcgatat ttttcccaat tcctgtggtc atatggtttt ctctgtcaaa
    17701 tacgtctttt tgaaattgtt tgtggtcaga cacaaaccca ttagtctcta acaatttcga
    17761 taatttttcg actacacttt ttttatcaga tgcatttaaa tctatcacta tattatttat
    17821 attgattttt tcactgatca tttataagcc ttctttcaat atataaacta atttttcaaa
    17881 cgacgaactc gtttttaaat ttgctaataa ctcatgattc gaagcgattt cattgaatgt
    17941 ttcataaatt ttattcaatt caggttttac tttttcatta agtgcaaaaa agaaaacaga
    18001 atgaacaagt gtcccatccc aatcaattcc ttctggatta acgtagacac agattactgg
    18061 cttttttata aaatctactt cgatatgcgg catagcaaaa tcattcatcg ctgttgaaga
    18121 tagtttttct cttttgattg ctccctctag tatcccttct ttcgcgtaat tttcttttac
    18181 caattttttg gtaattatat ttaaaacgtt cacttgatta tcctttattt ggtgggtaaa
    18241 aaagcaaaat cgtttatcta ataaatttag aaaagcatct tctgtttgtt catttaactg
    18301 tagatactgt tttacttttc tgacatctgc ccctaacatc aaaggactaa caacaataac
    18361 aggaaaattt gtattttcaa tcggaatcgt gcttataaca agttcatcag tgacttttct
    18421 atcttgtagt tcattgatgc ttagtacatc ttctatctca accatatctg caaaaccgtt
    18481 atttattctt tgctctaata attttgatgt tccatatccg ctactacaaa ctaatatcac
    18541 tttcgttttg tcaggtttgt aacgatccaa aagtgattga atatggagtg ttacgtaagc
    18601 gatttcatct tcattaaaat gtatacagaa tctctcttcg atttcctcta ataaatctac
    18661 acttatcata aatgaccttt taaaagaata tttgattttg tccgtataag ggttatagat
    18721 atttactccc aatttgagtc ttttaactgc tgcattgaga tgtaatacta gattattgat
    18781 caattcttca tcaggataaa attcatttaa tttaattctt actaactctt gtagctcaac
    18841 acccatatct aaataggaaa gcttcgtttg atctaagtcg tgattcatag catttttttc
    18901 aatcgaagta atgtgaatgt caacataagc ttgttcatct attggcacaa taattgaaaa
    18961 tgctttttca atcattttga ttaaaataag tgtattcttt gagatttctt cttcagaatt
    19021 attactacgt gataagaaat tgcttgtttc aatcctttct atcgcaatag caaggtggat
    19081 agctaaagtt tgaaattcat aatctgtaaa tttcaattgt gtcttattca tgtatcgatt
    19141 aagcaattcg gctacttttt tcaatatgtc cttatttaag aaattattcg cttcaccaat
    19201 gaaattgata ttcaagtcta agagatctgt tttactgtta gatgctgtaa agccacccca
    19261 ataatagttc acaacttctg acatcaacct tcttttactt ttttcatcag ctttgatgcg
    19321 cacgccattt cgatctgaaa ctaaggacat cccatgcttg tttagtcttt ttctaacatc
    19381 tttcattgtg ctttctaccg tcgacctgct cacgtataat tcgtcagcaa gattttgaat
    19441 ctttagagaa tcattagtat tcaataattt agcaaacaca taagttagac ggtcctccgg
    19501 agagtctgcg atattgattt tatgactgga taataaattt cgaagtgttt ctttttttcc
    19561 aataagcgaa acgccttttt taggttgaac caacatttgt acatctggca ttttttggat
    19621 tatttctttc aaaattaaaa tatctttgct aattgttttc gtagatacat ataattgttc
    19681 tgataattct ttgtaagttg tgtgctcatt ttcaatcaaa aagtatagta ttttcatttg
    19741 ccgttcgcta aattcgatgc ccattatctt cacctcctga atacagctta agtgtacaat
    19801 tattatctct attttcatac agaagaaagt tcttgattaa tgtagaagtt tctaatattc
    19861 ttgattattt caaatcaata aaaaagcctg caatatttat atcacaggct tttttctcta
    19921 ttaaatatat ataagataat acatttttct actagttctc actaatcttg tataattaaa
    19981 ccctccgcaa cttgacggag atggagaaga tgcccccgtc taatctagcg gtcatctctc
    20041 ctcccattcc ttccaccaat ttttttgaca ggtaaagccc gattccgcta gattccgtat
    20101 ttgtgcggct ggtattttcg gtataaaaac gctggaagac tttttcaaca gaggtttgcg
    20161 ttcctggttt gataggattg gccactgtta atcgaatgaa tggatcctct tttgaaaatg
    20221 tcacctttgc ttgtccatct gcataacgca ataaattctg tatgatattt tgcagcactc
    20281 gcagcaacat gagcggatct gctaatacag ttgtatttat cgagtgttca gcgaacgcta
    20341 cctcgatatt tttctcagaa aattgttcat aaaaagagaa aaatacttct tccacctctt
    20401 tagaaaaaga aagcgtatgg agatccattt ctttttgctt cgtctctatt cgagcaagat
    20461 cataaaactg atccgtcata ctgagtaatc ttgcaacaga ttgtttgatg atcgttaaat
    20521 acttctcccg tttctccgta tcctctacct gttccagtaa ttgtacataa ccgttaatag
    20581 cagtcaaagg agttctgaga tcatgagaaa ggccgacgat cgatagtctg atgttttctt
    20641 ccatttcttg aaatgcttgt cctttttgtt cattttcatc caaccatgta ttcagtgcat
    20701 ctactaggtc cattagattc ttttctcgaa attcgactga aagacgacca ccatattctt
    20761 gtcgttcaga aagagtggcg attttcttag ttaatctctt gatttccctt tgcaggaaga
    20821 aaataaaact tcccataaag ataacaacga gaagcaacag ccatttaatc aaatctaaac
    20881 cccactcccc acaccgtatg aatatacaca gcatgtgcct ttgctagctt ccctcgaaga
    20941 ttggaaatat gcatattcac tgttttctca ctatcaaaat atggttcttg ccaaactgtt
    21001 tcataaagat ttgctcgcga aaatactttt ttcggatgtt ctaaaaaaag cttcagcaga
    21061 tcgaattctc ttcctgtcaa atggacagcc acaccattca catttacatc cctcgactct
    21121 ttgtccagta agatttcctt gtaatagatg cgttgcgaca gatcttttac ttggacagaa
    21181 gtctgatgac gaagttggat gccgattcgt aacaaaagtt cttcgatatc aaatggtttc
    21241 gtcacatagt catccgctcc tgctgccaac aaggaaactt tatctctttg atcgcttttt
    21301 gctgaaagaa cgatgatcgg aacattcgat gtctgtcgga tttctgctaa caatgcttca
    21361 cctgaaagcc ctggaagcat gagatctaga aggatcaaat cgacaggata tgtgcttaac
    21421 aataattttc cttccgtacc tgaataagca tcaagaagtt tgtatctctg ttcaagaacc
    21481 tcctccagta aggaatgaac agcttcatcg tcttcgataa tcaaaatagt ttccagcagg
    21541 accacctact taatgtcaga tttttggaaa ataaacattc ctagagctga tgataccacg
    21601 attgttacta gactgaccag aatcattttc ggtgcttgcg aataagttgc ttgttgcgca
    21661 aagtccgtat acatgagcca cgtttccgta aaccactccg ttagaaattc caagcctttg
    21721 atttttgcga aaacaccggg aatgaaggcc gttcccatga tataaagaaa agaaagaagc
    21781 aaactgacac tggtacttct agtgatgaat gtcaaagcca ctggaacaga taatgtccct
    21841 aatgtcagca gaagcaatac aggaaatact tttagaacat aatttccata gtttttgatt
    21901 ccctcaactt caccaaatcc gaaagcaagt gtgtaagcaa gcatggctag tcccgtgatc
    21961 aaaaccacgc caagtgttgt gatcaaaagc gtaacgagaa gtttcgacac ataataagtc
    22021 attcgattcc gtcctaaaga taacgtattg cgaatcgtac cgttttgaaa ttcttcccca
    22081 atataaaagc acgcaaatgc tgcgaaagcc agaagaatcc atgcagccat tttttctacc
    22141 attaaaatcc ctgttgcacc gttgatgact gtatccgttg cttggtttaa ttcattgccg
    22201 tattttgtat gcatatacca ttcgttccaa gaataaaaaa gcggaagtac gaccatcagt
    22261 cctagtgaga cccacatttt acggctcttc catactttta ttttttcagc agttaataga
    22321 ttaaacatgt ggattgcctc cagttaattg taagaaataa tcttccagtt tttgcccaac
    22381 taggtgaata ccatccacag gaacttttgc ttctaccaat gcttgattga tggctgctac
    22441 ttggtcaatt ccttcaaaaa gctggataac acggctagat tgtacaaaat aatccctata
    22501 accaagagag tcgagaaccg ttactgcttg ttccgtcgcc gaagtgtcaa tttttataaa
    22561 ttgacgagat tcttgtgcca attcttcttt cgataattct ttgatcaagt gtccttcatg
    22621 caaaatacca tagtgagtgg cgatctgtga caattcatct agcaagtggc tagaaagcaa
    22681 gacggtgatg ccccgttctt ttaccaaacg ttggatgatt tctcgcattt ctgccatccc
    22741 tgctggatcc atcccattcg tcggttcatc caaaatcaaa aattctggat tcgtgatcag
    22801 tgcatttgca atagctaaac gctggcgcat acctaaagag aaattctttg ctttcttttt
    22861 ccctgtattt tccaatcgca tcattttcag caggtcttcg atttctctat ccgacacacc
    22921 gccgttcgct gcttggatac gtaggttatc tcttgcagtt aattctggat aaagagcagg
    22981 ggtttcaatc gattgaccca tacgtcgtct tgctgcctgc aaatccgtcg ttccaaataa
    23041 ttcgatcgaa ccttcatcaa tcgagatcaa acccataata gttctcataa aggtagactt
    23101 acccgctcca ttttccccaa tcaatccata gatcattcca cgtttgattt cgattgatac
    23161 tttatctaat gctttggcag caccgtattt tttggttata ccagttgctt tcaagaccgt
    23221 ttcatttcca aaatccatac ttatccttct ttcttattga tgagtgaagt atagttgacg
    23281 ggtataaaga aagcagccag aaatgtataa agaaagtgta aattttccaa ttgaatgtca
    23341 aaaaaaacaa tcggacaata cgtgtctttc ctattatcag gcagtttgtt atacgcttgt
    23401 atcagtttcc tgattctttc ttttcgacca aagtcgtttc tctgtattcc tcttcactga
    23461 aaatttttgc ttctttcaga tcaagcttga gtaattttac cagaccggtt ataaggataa
    23521 ctatagcaag actccctccc agaaaaatta gactgtctcg cagacgtggt tgcgttgtct
    23581 tccaatcaaa gagcgcacca aacagtgcta atccaagtgg actggccacc atggtaatcg
    23641 ttgttatcgt tgacataagt cgtccttgta tatgcgttgg aacttctcgc tgaagccaga
    23701 ccatataaaa agcaaaagaa aacattaatc cttaaaaacg gacttatgaa aaataataca
    23761 tgccgtttgg gatttacaat tagctaccaa ataagaggtt atgacaaaag acttttcaca
    23821 atcccttctt ccgaataaac ggtgttctcc tgccaactcc tcggtcacaa gtcacaatat
    23881 ttatcaaaca tgagaagcaa tttttgtaaa ttccttctta tttcttgaag ctgaccactt
    23941 ctgtcacaac ctcagaagcg gataaataat attgtttgat gatactttat ctttttcccg
    24001 tttgtttata gatcgaatga tattatagac caagaatcct acctattaac atagctaaaa
    24061 acacaaaaaa ggcactttcc aaattgtgga aagtgccaga aaagttagga ttaaggtaac
    24121 tgcgctcagc attgcttcgc ccagtacgat tctcgtaact cgtgaactcg aacgataatc
    24181 gcaattaacg ttttgagaac tgtgaagctt tacgggcttt cttaagacct ggttttttct
    24241 tcgtatcatt gaataagttt gaaaaacctt gatttataaa ggttttcccg taaatatatt
    24301 ttattatatt tgattaaatt tgaaatgttt gtgttctaaa atgtgttcta atttaaaggc
    24361 agtatatttc gtattttaga ttaaattttt aggctcattc attctcaaat ccattctatt
    24421 cagtcaataa aaattttaaa aagactttat aaacaaagat gttcaagcga gcatcttatt
    24481 tttttgtgct taatttgaaa ggagaacatt tcatgtataa aaaacaaaaa aagaaattat
    24541 gtgtattaac tattttgagt ttggctgttc aagcaattgt tggagcaatt gctcctacga
    24601 ttacttttgc tgatgaaatt acccatccac aaacggtaac cgtagaacta gatttagcac
    24661 atcaatatgc tgttgaagga acttttagtg atggtcgtcc aatgtcagaa gtcactgttc
    24721 cgcattatgc agtgtacaac ggctttaaac aagatatttt ctgtattgaa ccaggagtcc
    24781 cgatttacaa tgagttcact cctggctatg agaaaaaccc gttaccagat atgtccgata
    24841 aagccaaatt agtttccgtt ttatggaaaa aagtaggtac agatgcagat actcatattg
    24901 tggcacagaa gatgatctgg caagaagtta atgggtatac ccttcactca ataaaacgat
    24961 cagatggtag tgccgtaaat atcgcagcca ttgaggcaaa aataaatcaa gccattgacg
    25021 attatcagaa gaaaccaacc ttccataata gtacggctaa aacagtatta gggcaatcga
    25081 ctactgtgac ggatacgaat aatctgaatt tgtcagagtt cgatgaggtt gtggaaaatg
    25141 ccgcaaacat tgattatcgt gtgaatggta atcagttgat aatcacccca aatgccaatt
    25201 caaaagaaag tggtgtgctg acgcttaaga aatctgctgg taccggaaca cctgtcgcct
    25261 ataaaaaggc tggccaacag actttaatgg ccggggcgat tgataaacct aatacctaca
    25321 caatcaaaat tgatgtggaa acggagggtt ctttaaagat caaaaaagtc gataaagaat
    25381 cgggtgatgt tgtaccagga acggttttcc atttagactt tggaaaaact ttacctgcaa
    25441 aagacgtgac tactgacaaa gaaggcattg ctacattgga tgagattcct catggtacaa
    25501 aagtaaccat taccgaaaaa tcagtaccgg cgccttatac gattgatacc acccccatga
    25561 ctaccaccat taaagcaggc gaaaccattt acgtgacttc aaaaaacgca cgggaaaaag
    25621 ggcaaatcat tctggataaa agcggagttg aaacagggag tgatctttgg aatgacaatt
    25681 attccttagc tggtaatacg tttgccattc gcaaagacag tccaaccggt gaaatcgttc
    25741 aagaaatgac cacggatgaa aacggtcatg cggaaacacc aaaagagatt gctaatgcgt
    25801 tggaattggg aacctattat gtgaccgaaa ctaaagctag tcatgggttc gtgaatacgt
    25861 tcaaacctgt aaaagtcgaa ttgaagtatg ccaatcaaac cgtagcccct gttaccagta
    25921 acgtaaaagg gcaaaatcaa gaagtcactg gcgaaaccac tttgacgaaa gaagacaaag
    25981 atactgggga taaagcacaa ggtaaggcag tctttgaggg tgctgaatac accttgttta
    26041 ctgctaagga tggaaaagct gttaaatgga gtgaggcttt taaaccagag ttagcgaagg
    26101 gaacgaaagg ttctgatgaa acggtcactt tgactttaga cgaaaagaac caagttgccg
    26161 ttaaacattt agctatcaac gagtattatt ggcaagaaac aaaagcgccc gaaggatata
    26221 gccttgatga aacaaagtat cctgtatcca tcaaaaaggt ggatgatagc gaaaaaaatg
    26281 ccgtgattac ccgaaatgtt acggcaaaag aacaagtgat tcgctttggc tttgatttct
    26341 ttaaatttgc tggatcagct gcgggcacta ccgaaactgg cttcaatgat ctgaccttta
    26401 aagtgtcgcc attggaaggg accaatgaaa tcacaggtgc cgaagatgaa gcgaccacag
    26461 cttataacga gcaattaggg tttgatggtt atggcaagtt tgaaaatctt ccttatgggg
    26521 attatttact tgaagaagta gaagccccag aaggttttca aaagattacg ccattagaaa
    26581 tccgttctac ttttaaggaa aacaaagaag actttgtgaa gagtgagtat gtctttacca
    26641 ttaccgaaca agaccaaaaa caaccaatta aaacggtgac cgttccttac gagaagctga
    26701 cgaacaaagc attttctgtt agtttgaacc gtttaatgct ctatgatttg cctgaggaag
    26761 aagatagttt aacttctctt gcgacttgga aagacggaaa caaagaatta acttctctgg
    26821 attctaccga gctagttgat aaattgagtt acaatctaca tgaaatcaaa gaagactggt
    26881 atgtggtagc gcaagccatt gatgtggatg cgacaaaagc tgcccaagaa aaagatgaaa
    26941 aagccaaacc agtggtgatt gcagaaacaa gcgcaacgtt ggcgaacaaa gagaaaacag
    27001 gaacttggaa aattcagcat aaattaaccg ccgaacaagt tttaaataaa actattgtct
    27061 tattcaacta tgtgtatgaa aataaggaag cttttgaagc aggcgataag ccagtagcga
    27121 aggatgttag cttgaataac caagcccaaa ctgtcagttg tacagtggaa caccatgttt
    27181 ctatccaaac aaaggcccac ctagaaaacg gttctcagac ctttactcat ggtgatgtgg
    27241 tggatatgtt tgatgatgtg tcgattactc atgatgtact ggatggctca aaagaagctt
    27301 tcgaaacaat tctgtatgct ttattaccgg atggtacaaa caaagaaatt tggaaatctg
    27361 gcaagattga ttatgaagta aatgataaag aattcaccaa aactgtgctt gcgaaaaaag
    27421 tagataccgg aaaatatcca gaaggaacca agtttacttt tgcggaaatc aattacgata
    27481 aagatgggaa tatcaatgga aaacacaatg aagatttgaa agaaaaatct caaaccttaa
    27541 caccaaaaga agttccaacc atactaagta cgccgaaaca accggaaaca ccaactgttc
    27601 caagtggctc tcaagaatct agtcccacag tgaagacctt ccctcaaacc ggggagaaaa
    27661 attccaatgt tttactgttc attggtttta ctttgatttt tgcgacggcg ggctattatt
    27721 tctggaatcg ccggaactaa ggtgatgaga tgatacaagt aaatcctaag aaccaacgtg
    27781 cgcgaccgcc tcctaaaaaa gtagtcgcgc tatttttgta tccaaaacta acaatgaaaa
    27841 gaggaaaaga agatggaatt gaaatttgtc gtgccagata tggctgaaac ctttggaaag
    27901 ttaagttatg ccggtgaagg agaaattata acagaaggat atggacggaa tactacggtg
    27961 attggtcgca gttatcattt gtattccagt aagcaacggg ccgatgatat tgaggtggta
    28021 gtagctgcgg aagccggtga aaaggatttt gatcaagacc aaccgctaaa agccgtgaat
    28081 ccccatttgg ttgccaaagg ctatgagatt gagaatcgtg ggtttactga ttacgtgttg
    28141 tatgttgatg atttagtgaa agcataggag gaaaagaaga tgagattaac agaaggtatt
    28201 gtcgtagatt caggtttaac atttgggaag ttgcgttttt ccgcattacg ccgagaagta
    28261 cggaagcaaa atgaggatgg aacaattggt aatgaggtaa aagaacgaac ctataattta
    28321 aaatcctctg cacaagggcg aatgattcaa gttagtatcc cagctaatgt acccttacgc
    28381 gagtttgctt atgatgcaga ggtagagttg gtcaatccaa tcgtggatac cgttgccaat
    28441 tatgtttttc gagaaggaac taccgttaat tggttcatta aggcggatga tttagtcttg
    28501 aagcggcagc cgaatcaagg aaatcctacg aaccaaaatg aaagaaagaa ataggtgaag
    28561 attatggaaa ttaatgttga acatatttta gattgtctcg atcaatatgg aaaaggcgag
    28621 ctgactgagg aacaactcgt taaagcatta acctctgatg aaaaaatgtt tctgattata
    28681 catcaaggtt tacttggaga aaatagcgac agtgaagaag actctgatgt actagactgg
    28741 ttaggagaag aagagtcttt ctttatagtt gtcgaagtaa atgaaaactt atgtcggcag
    28801 gcagaatcca ttttggaaga aattggtgtg gagatgccgg atgctattga aggcttttta
    28861 aatcaattgg ttgaaacgaa acaactcccc tcctcgtagc agtaaatgac tagcataact
    28921 ttttgaaatt gcctaatgaa atcctattgg gctttttcga ttggaggtgg aaaccatcaa
    28981 aatgtacaaa ggacatcgca tacgcgcagg agatcagcac ttggtttatc actttgttct
    29041 tggctggcta ctcgctctgt tcatcggttg gatgagcgtc ttttattttc aagagtttag
    29101 acaatttgat atttctaagc tatcactttc tactattgaa atcgtttggt ctataaaaga
    29161 tttagtatgt ctgcttggaa gtctcgcttt ttcaggagct atgatattgc tttatattca
    29221 cttttttctt gatcattggc gaagcttgtg gcatcgacaa aagctagctc ggatgattct
    29281 agagaaccat tggtatgaag tgaaacaaac tcaaagtgaa ggctttttca aagatttgaa
    29341 tagtagccga accagagaga ctatcagtta cttccccaaa atctgttatc gaatgaaaga
    29401 tggattactg tctattcgcg ttcaaatttc acttgggaaa taccaagatc agcttttaaa
    29461 attggaaaag aagttagaaa gcggactgta ttgtgagtta gtagaaaagg aactcaaaga
    29521 ctcctatgtg gagtatactt tactgtatga tatgatcgcc aatcgaattg ggatagacga
    29581 agtagtggca gaaaatggga ctctgcgatt gatgaaaaat caagtatggg cttatgattc
    29641 tttaccccat atgttaatcg ctggtggcac gggtggaggg aagacctatt tcctcctcac
    29701 cattatcgaa gcactattga agtcagatgc agaattgttt atcctcgatc ccaaaaatgc
    29761 agatttagct gatttaggta cggtgatgcc tcatgtttat tcacaaaagg aagaaatttc
    29821 tgcttgtgtg gaagatttct atgaacgtat gatggctcgt agcaaggcaa tgaaagaaat
    29881 gtctaactac aaaacaggag agaattatgc ctatcttgga cttccaccca actttttaat
    29941 ctttgatgaa tacgtggctt atatggagat gttaacgacg aaagaaagtg cggtgatttt
    30001 gaataagctc aaacaaatcg tgatgttggg tcgtcaatct ggtttctttc tgattttggc
    30061 ttgccaaagg ccggatgcga aatatttagg agacggaatc cgcgatcaat tcaactttcg
    30121 cgtggctttg ggtcgtatga gtgaactcgg atattccatg atgtttgggg aggtcgaaaa
    30181 aaacttcttt atgaagcaca tcaaaggtcg aggttatgtg gatacaggtg gcagtgtgat
    30241 tagtgagttt tatacgccac ttgtaccaaa agggtatgat ttcttagatt caattagtaa
    30301 attagaaaag atgaatgatt gagtgatctt caatactgac tttatacttc aagtaacgta
    30361 taatactttt atatagtagg aagaaagggg ataattaata tggaaagtaa tgattcttat
    30421 ttagagtatg caaaagcaaa taaggataag tttatttctt atattatcaa agggaaatcc
    30481 cccgaagttg gacgagacgc agtttttatg gcagggagtc ctggggctgg aaaatcagag
    30541 gttgccattg gattagctga gaattatgat aaccatgtga ttattgatgc ggattacttt
    30601 cgcacccagt ttcctgatta taatggaaaa aactctagtc tctttcaaaa agcatcttct
    30661 tggttggtag agcaggcatt tagatatgtc ttagatcatg gctattcttt tattctggat
    30721 gcaacttttg caatattaag tgctgaaaaa aatatctcgc gggcgttgaa aaaacatttt
    30781 cgagtgacga ttttctatgt ttatcaagac cctaaaatcg cttggaaatt tacacaagaa
    30841 cgtgaaattg cagagggacg tcatgtgccg aaaaaggcat ttattaacgc ttttttcaaa
    30901 tcaagagaga acattgcaaa agtaaagagt aaacatccag atgtattgct tcatatcata
    30961 ataaaagatt atacgaataa tatttccgaa gttcattatg ctacggataa tctagaatta
    31021 tttttaccac tagggtatag taggaaagat ttggaggttc aattacatga ctgagaaaga
    31081 acaagtaacg aaaattgtaa agaaatacaa taagagcatt gcggatttat cggaaaatgc
    31141 tactgcaaaa gagttcaaaa cggtgattaa atatgtagcc gatcaggcca atgaaaaaca
    31201 acggaaatta gttggattaa ataaaaaata gtaggacaaa ttaat
//
LOCUS       DAJSBL010000030        29366 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.030, whole genome shotgun sequence.
ACCESSION   DAJSBL010000030 DAJSBL010000000
VERSION     DAJSBL010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29366
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.030"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(126..974)
                     /locus_tag="ONT85_001626"
     CDS             complement(126..974)
                     /locus_tag="ONT85_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCR2378603.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            complement(1198..3138)
                     /gene="thrS"
                     /locus_tag="ONT85_001627"
     CDS             complement(1198..3138)
                     /gene="thrS"
                     /locus_tag="ONT85_001627"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HCR2378604.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(3475..3654)
                     /locus_tag="ONT85_001628"
     CDS             complement(3475..3654)
                     /locus_tag="ONT85_001628"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HCR2378605.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(3701..5524)
                     /gene="cadA"
                     /locus_tag="ONT85_001629"
     CDS             complement(3701..5524)
                     /gene="cadA"
                     /locus_tag="ONT85_001629"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HCR2378606.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            5802..8081
                     /locus_tag="ONT85_001630"
     CDS             5802..8081
                     /locus_tag="ONT85_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR2378607.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(8174..8821)
                     /locus_tag="ONT85_001631"
     CDS             complement(8174..8821)
                     /locus_tag="ONT85_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2378608.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(9000..9719)
                     /locus_tag="ONT85_001632"
     CDS             complement(9000..9719)
                     /locus_tag="ONT85_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR2378609.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(9850..11001)
                     /locus_tag="ONT85_001633"
     CDS             complement(9850..11001)
                     /locus_tag="ONT85_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HCR2378610.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(11218..11958)
                     /locus_tag="ONT85_001634"
     CDS             complement(11218..11958)
                     /locus_tag="ONT85_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCR2378611.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(11962..12285)
                     /gene="rsfS"
                     /locus_tag="ONT85_001635"
     CDS             complement(11962..12285)
                     /gene="rsfS"
                     /locus_tag="ONT85_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HCR2378612.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(12342..12935)
                     /gene="yqeK"
                     /locus_tag="ONT85_001636"
     CDS             complement(12342..12935)
                     /gene="yqeK"
                     /locus_tag="ONT85_001636"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HCR2378613.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(12919..13569)
                     /locus_tag="ONT85_001637"
     CDS             complement(12919..13569)
                     /locus_tag="ONT85_001637"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HCR2378614.1"
                     /translation="MMNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHL
                     VMAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIEL
                     ARGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPG
                     YTTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(13579..13890)
                     /gene="yhbY"
                     /locus_tag="ONT85_001638"
     CDS             complement(13579..13890)
                     /gene="yhbY"
                     /locus_tag="ONT85_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HCR2378615.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(13906..15015)
                     /gene="yqeH"
                     /locus_tag="ONT85_001639"
     CDS             complement(13906..15015)
                     /gene="yqeH"
                     /locus_tag="ONT85_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HCR2378616.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNETQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(15012..15539)
                     /locus_tag="ONT85_001640"
     CDS             complement(15012..15539)
                     /locus_tag="ONT85_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HCR2378617.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(15719..16504)
                     /locus_tag="ONT85_001641"
     CDS             complement(15719..16504)
                     /locus_tag="ONT85_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HCR2378618.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(16497..17366)
                     /locus_tag="ONT85_001642"
     CDS             complement(16497..17366)
                     /locus_tag="ONT85_001642"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HCR2378619.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(17367..18746)
                     /locus_tag="ONT85_001643"
     CDS             complement(17367..18746)
                     /locus_tag="ONT85_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HCR2378620.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(18754..19173)
                     /gene="fabZ"
                     /locus_tag="ONT85_001644"
     CDS             complement(18754..19173)
                     /gene="fabZ"
                     /locus_tag="ONT85_001644"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HCR2378621.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(19198..19674)
                     /gene="accB"
                     /locus_tag="ONT85_001645"
     CDS             complement(19198..19674)
                     /gene="accB"
                     /locus_tag="ONT85_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HCR2378622.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(19677..20912)
                     /gene="fabF"
                     /locus_tag="ONT85_001646"
     CDS             complement(19677..20912)
                     /gene="fabF"
                     /locus_tag="ONT85_001646"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HCR2378623.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(20931..21668)
                     /gene="fabG"
                     /locus_tag="ONT85_001647"
     CDS             complement(20931..21668)
                     /gene="fabG"
                     /locus_tag="ONT85_001647"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HCR2378624.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(21680..22597)
                     /gene="fabD"
                     /locus_tag="ONT85_001648"
     CDS             complement(21680..22597)
                     /gene="fabD"
                     /locus_tag="ONT85_001648"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HCR2378625.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(22704..22928)
                     /locus_tag="ONT85_001649"
     CDS             complement(22704..22928)
                     /locus_tag="ONT85_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HCR2378626.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(22981..23943)
                     /locus_tag="ONT85_001650"
     CDS             complement(22981..23943)
                     /locus_tag="ONT85_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HCR2378627.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(23953..24393)
                     /locus_tag="ONT85_001651"
     CDS             complement(23953..24393)
                     /locus_tag="ONT85_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005471259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCR2378628.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
     gene            24699..25664
                     /locus_tag="ONT85_001652"
     CDS             24699..25664
                     /locus_tag="ONT85_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HCR2378629.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
     gene            complement(25721..25951)
                     /locus_tag="ONT85_001653"
     CDS             complement(25721..25951)
                     /locus_tag="ONT85_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HCR2378630.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(26037..26924)
                     /locus_tag="ONT85_001654"
     CDS             complement(26037..26924)
                     /locus_tag="ONT85_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HCR2378631.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            complement(27013..28236)
                     /locus_tag="ONT85_001655"
     CDS             complement(27013..28236)
                     /locus_tag="ONT85_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010706866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HCR2378632.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            complement(28253..29299)
                     /locus_tag="ONT85_001656"
     CDS             complement(28253..29299)
                     /locus_tag="ONT85_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HCR2378633.1"
                     /translation="MKVLLYFESEKMLAKSGIGRALDHQKRALTEVGISYTLDEKEDY
                     DILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQLSPIVKKYLVGL
                     YQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQKFREYFKLSPD
                     QKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIRKIVKEDHPENV
                     LFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVRDIPVYNGWLED
                     SKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEELKNVYESVLNE
                     KVSSKLKKVNQVKD"
BASE COUNT         8144 a         6424 c         5060 g         9738 t
ORIGIN      
        1 taaaaaataa acttattttt gaaaattatg ttttcttaag tcgtgaaaca ctttgatgga
       61 tcatttattt gtggaaaact acgttttgtt attctaaaaa acttatataa aaaatagtaa
      121 ggtttttatt tcgaatccat aaaatattct tggattgctt ctcgtgtttt tttcactttt
      181 tcgctaagct tttcgttttt agccatatag aaataaaaaa tgatatcgat cgtagcaaat
      241 tgggccaaaa gtgaattagt tgccgcactt cgattcgtga tttcttttgg tctagatgtt
      301 tgtacgagta catcggctat cttgctaagc gggttatttc caaattgcgt caacgcaatg
      361 atttcgatat tcatgctttt cgccagctcg gctaatgcga ctacatctgc acttctacct
      421 gagtttgaaa caagccaaag cactttcttt ttctcattgc tgacaagttg cgggagcaag
      481 acgtggatat ctttttcaaa aatgattggc ttaccgacac gagaccattt ttgcagaata
      541 tcttctacta ctaaagaaga ggcgccaacc ccatataaaa aaacagtctc tgccgattcc
      601 aaacactcta cgactttttg gatcgtttct tcttctagaa tatctactgt ttcttttatc
      661 gctacttgtg cattgttacc aagtttgttt ttgatagagt aaaaggactc atttgcttct
      721 atatcactaa aaccttcatc gattccagtc tgtagttctg ttgaaatcgt gattttgaag
      781 tccgtcagac tttgaaaacc gatttttttg gaaaaacgta caactgtcgg cgcactcaac
      841 ccggaagctt ctgctaattc attagctgtc attgtaggga ccttctcaat attaccgata
      901 atgtagttgg ccaacttttt ttctgctgaa gaataatctt ccatcatact ttcaatacga
      961 ctaattacgc tcataagttc ctccaccctt ttaatgatag tctatgtaag tataccatat
     1021 cagaccctta ttcacggtcg tatactggat aaaaaacaaa aaaacaatcc gcccaacaga
     1081 atatcttatc ccatcaatcc cacagattct attgagcatc tgtgtctttg aggaatacct
     1141 gttatctatt gttcggattg ctttctatct actacttttt tacaggaaat ttacctatta
     1201 gttttctctg ctgtaatttt tgacttctgc ttccatagca tcaataaaga tattcaaatc
     1261 ttctacagct gtttctttac ttccataacg acgtacgttc actgttgcat cttccaattc
     1321 cttgtctcca acaactaatt ggtaagggat cttttgtgtt tgagaggcac gtatcttata
     1381 gcccattttt tcattacgat catcaacttc tacacgcaac cccttcattt gcaaacgttc
     1441 tttgatttca taagcataat cgccatgtgc atcaacagaa acagggatga ttgttgcttg
     1501 tataggtgcc aaccatgttg gaaaagcgcc tttgtatact tctgttaaat aagcgacaaa
     1561 acgttccatt gtagaaacaa ttccgcggtg gataactact ggacgatgcg tattttcccc
     1621 gtcttctcca acatatgtca aatcaaagcg ctctggcaat aagaagtcta attgtatagt
     1681 tgataatgtt tcttctgtac caagagctgt tttgacttga acatctagtt ttggtccgta
     1741 aaaggctgcc tcgccttctg cttcaaagta atctagttct aattcatcca tagcggcttt
     1801 cagcattgct tgggcttttt cccacattgc gtcatcatca aaatatttat ctgtattgtt
     1861 tggatcgcga tagctcaagc ggaaacgata atctgtaata ttgaagtcag catatactgc
     1921 caccatcaat tcaagcgtac gtttaaattc atctttgatt tgatcaggac gaacaaatgt
     1981 atgaccatca ttcaacgtca tttctcgtac acgttgtaaa cctgacaaag cacctgattt
     2041 ttcatagcga tgcatcattc ctaattcagc gatacgaatt ggtaattcac gataagaatg
     2101 gatcgtgttt ttgtagacca tcatgtgatg agggcagttc attgggcgaa gaacaagcat
     2161 ttcgccatct cccatatcca ttggcgggaa catgtcttct tgataatgat cccagtgtcc
     2221 agatgttttg tacaactcaa catctcccat tattggtgta tatacatgtt ggtaacctag
     2281 actaacttct ttgtctacga tataacgttc gatcgtgcgt cggattgttg ccccttttgg
     2341 aagccagaat ggcaaaccag aacctacttc tggggaaacc atgaacaagt caagttcttt
     2401 tcctagttta cgatggtctc tttcttttgc ttcttcacgg agacggatat attctttcaa
     2461 agctttcttg tcaaaaaagg ctgtcccgta tacacgttgc atcatttgat tgtttgagtt
     2521 tccacgccaa taagcacctg ccactgataa taatttaaat acttgtatac ggccagtgga
     2581 tggaacatgt ggtccacgac acaaatcgat gaattcgcct tgttgataag cagtaatcac
     2641 ttcttcttct ggcaattcac taatcagttc tactttgtac ggatcacttg caaaaatttc
     2701 taatgcttcc tgttttgaaa gaacacggcg ttcgatcggc agattttctt tgacgatttt
     2761 catcatttct gcttcgattg ccggcaaatc ttctgcagtt acttggtttt gtccattatc
     2821 tgtatcgtag taaaaccctg aatcgatagc tggtccaacg ccaaagtgga tatttggata
     2881 gaggcgacgc atggcttggg ccatcaaatg agctgctgaa tgacggacta agccaagtgc
     2941 atcttcatgg tctggtgtga tgatctcaat acttgcatct tcctcgattg gacgagtcaa
     3001 atcaacgagt tttccgttca ctttaccagc taaagctttt tttgctaagc tttttgaaat
     3061 gctttcagca attgccaatg ttgttgttcc agactcaaat tctttgactg caccatctgg
     3121 aaaagtaatt tttatcataa tagtttcctc cttgaaatgt tttgattgat cagatcatca
     3181 agctgaaaaa caaaaaaatc ctagcatctc ttgaaaagag atactaggac gaaaagttcg
     3241 tggttccacc taatgttcaa acgcaaatgc gttcctcaag cgttgtaacg gtccgaccgc
     3301 ccttgcttca acaagggttt cgaaagtggt catcctttgg ttttctcctg gaaaagtttc
     3361 agcctagctt ttcctctctt atcgaagact ctcaaattca gttggctttc tcatcaatca
     3421 attatgtgtt tatttaacca ctaataaata gaaaaatcaa gtgggaaact aaacttattt
     3481 tttccattcc ccatttacac cgtatgttcc ttttttcatc tcattgatga tgacatggat
     3541 gtgttctttt ggtgcctgcg tgtttttatg aacagcttcc gtgatatctt tcatcatatt
     3601 ttctaattgt tccggactgc gaccttctac taattcgaca tgtacgaatg gcataatgct
     3661 ctctcctctt tctgatcgtt cttttttcaa ctcgattcta tcatactatg tttgtcttgg
     3721 gtactgaatc aggaaaaatg gctggatctt tttttgctct caaggcactc aaaatagaga
     3781 ccgtatccac gacttcttgc aaaacagctc cgaataatgc ggggattagt cccgtacttg
     3841 caattaacat caaaaaaata caaataaaga tgccaatcag tacagactgt ttcgctattc
     3901 tcatcgtatc tcttgaaatc actactgctt ttgctacacg caagagatca tctttcaaga
     3961 tgaccgcatc tgcactttca ctagcagctg tagaaccatg agcccccatt gcaataccga
     4021 tatctgcagc agcaagtgct ggtgcgtcat tcacgccgtc tcctaccatc gcaacaggtc
     4081 tttgatcttt agggatactt tctaaaatgg tcagtttatc ttgaggcaag ctacgggcat
     4141 gtacagaatc taattggact ttttctgcaa tcgtttgagc aatggcagat ccatccccgg
     4201 tgatcatcat caaatttttc acatcaagtt ttcttagctc cttgattgtc tttgctgctt
     4261 ctgggcgaat ctgatccgaa aataggatcg ttcctgcaaa agatcgatca attgttacat
     4321 agattgctgt attggacgtt tctatttctt cagctgaaac aaaagaggca cgtccaatct
     4381 ttacttcatg attattgact actgcttgga ttccttgacc ggtcacttct ttcagttgac
     4441 taacaggata cagttggctg tttttcacgt tgttcactaa cgaacgagct aagatatggc
     4501 tagattcctg ttctgcacta gctgcataca gaagtagctc ttcttttttt attccattga
     4561 ctggctgaat cgcctctact tccaatatcc ctttggtaag cgttcctgtt ttatcaaaag
     4621 caatcgtttt tgtacgtgcg agcttttcaa tcgttgttcc agtttttaca acgatcccgt
     4681 ttttacttga acggctcatt cctgcaacta aagcaatggg cgcagctaga atcagtggac
     4741 aaggtgatgc cactaccagt acctcagcaa atcgtaaagg gtcttttgag aaccaccaag
     4801 aaataccacc gatcaaataa gcaatcaacg tgaaagggac tgcataacga tcagctaaac
     4861 ggacaaatcg ggcaggcttt tcttttgatt cttcgactag tttaataatc atttgatact
     4921 ggctgtctgc ggcacgtttt tcagcgatca tttggatcga tccgtctcca tttaaagacc
     4981 ctgacataat ctgatctcca ggtttcttgg tttccgggcg ggactcacct gtcaaagaag
     5041 attcatcgac cgaagattct cctttggtca ctcgaccatc tgcgggtaca agttcgccag
     5101 gcttgactag taactggtca tcaatattta tttcatcgat tactatatcc tgcaattttc
     5161 cttctttgaa tcgatgggca attcttgggg tgttatctaa caaagactgc agttctttac
     5221 ccgctcgttt attcgcgtaa tcctctagac tatctccgcc cgtaagcatg atcaatatca
     5281 tcaaactagc ccagtattct ttgacggcta atgttgcaag gattgcggta atcgctaata
     5341 catctacgcc atatcttcct gaacgtaacg tcttgatcat ttccaaaaac atcaagaagg
     5401 cgagaatccc tccagtaaat gcgacaatcg caaatgcaag ggccggctgg tgaaaaacaa
     5461 attcacttaa taaagctatc ccaccaataa gcaaagtaag taaaaatttt ttgaaatgtg
     5521 tcattgtttt ttctcctctt ttatgtgact tagagaggtt agtaataaat ttccagctgc
     5581 ctctttcatc ttaagcatag tccttgacta gcacttttta aaacaatagg ccttaattga
     5641 aaacaactat cactatcagc gtttccaaaa ttcatcagtt ctacaaacaa aaatattcag
     5701 caaggattac tttttcgatt catcagttaa aaagtacctt ttttcgaaaa actatgctat
     5761 acttttaatt cgttatttac ggcggaagga gcaaacatgc gatgaatgag aaacaactag
     5821 aacaaacgca cgtggatgaa acgatcgaac taatccagat cgaacaaaat atgctggata
     5881 caaaacaaca agaattgact caggaaatga atcattcgac aaaagacaca gctaatcaca
     5941 agattcgagc tggttccaat gaagcttttt atgaatctgc tgttgaatat cgccaacatg
     6001 agcaggaatt gcttttgaaa taccatactc ttgaagcaca gcaaaaacgc ctgcaaacgt
     6061 tgtctgtcat gaaagatagt ccttattttg cccgtatcga ctttaaagaa gaccgtgaaa
     6121 cagaaacatt atatctaggt atcgcttctt taagagatct ggcagaagaa acaatcgtta
     6181 ttgactggcg cgcaccaatt gccaatcttt attatgaagg tgagattggt cccgcatttt
     6241 atgaaactga aacagataga atagatgtcg atctgcagtt gaaacgacaa tttaaaatcc
     6301 aagaaggacg tattttatct atggtagata cgtcggaagt aatcaatgat gactttttgc
     6361 tagagatatt ggatgaatca tcaagcaacc atatgaaaaa catcgtctct acgatacaaa
     6421 aagcacaaaa tgcaatcatt cgggacacaa caagtaaagt aatgctgatt gaaggaatcg
     6481 ccggaagtgg aaagacatcc gctttgcttc aacgtattgc ttttctgttg tatcataatc
     6541 gcaagtggct cgaggctgaa aacgttcttc tattttcacc caaccatttg ttttctgatt
     6601 acatctcgac tgtattgcct tcattaggtg aaagcggcgt gcctacacag acattcaaaa
     6661 attatttggt gcagctgctg ccagagtttt ctttactaga tgaacagcaa caagaaagtg
     6721 gttttttgct aggggaaaaa gatccgatac aagtgatgaa aagcggattg accttagtcg
     6781 atcagattga tcgctatatc caaggcatca caaactacgg tcccttgttc cgtgatatga
     6841 aaataaatgg ccgtacgatc ctatcaaagg aatcgattcg ccaatggtat aaagaaacaa
     6901 atgaacaatt acctttacat catagacttt ctctattaca aacgaagctc ctgaaaaaat
     6961 taggcggtct gcaaaaagac gaaacacgtc agcaatgggt caaagatctg gcggaagaac
     7021 agctccaaga actatacgca acagatccaa atcttgaata tacagaacaa aaggagcgtt
     7081 cattacgtaa aaaactggcc aaccaaatcg tacgtaaaag attccgtcga atccatcgag
     7141 ggatcatgaa ttatcaattt gtcaatataa cgaaacaata tctccacttt ctacaaactg
     7201 tgccactttc tcttctaaca cgacataaaa tcagcgagac caggtggcag gaacatttga
     7261 caactgttcg tacacacttg cgtcaaagag aattgcgtca agaggatgct gtactattct
     7321 ttttactgat gcgccgaaca tatccagtga cggttacaca caaagctcgc tatatcttta
     7381 ttgatgaaat gcaggatttc gcaccatcac aagtggcatt gctgcaagcc ttgttcccaa
     7441 aagcaaattt gactttctgt ggagatctaa atcaaaatgt ctttggaaat gaaacgatcg
     7501 ttggatcatt ggaagacctt ttcgtggacc gtgaagtcac tcgtttccaa ttaacgacaa
     7561 gctatcgctc cacaaaagaa atcactgatt ttgctaatca ttttattgtg aaagaacatc
     7621 aagtagaaac aacagcaaga aaaggagacc ttcctttgct catccaagga acaacagatg
     7681 atatcaaatt gaattggtta gcagatacca ttcgaacgac tcagggaaaa gcccggtatt
     7741 ggcgtacagc aatcatagct aaaacacaag aagaatgcga agctctttat gctcaactgc
     7801 cagacgagtt gaaacaacaa gtccagctga ttttaaatga gagtgacttt atgaagcgtt
     7861 ccattatttt acttccagcc tttttagcaa aaggtctgga gtttgaccga gttttcttgt
     7921 gggatgtaga tgcagaacat ttttcttctc agcaagacaa attgatcctt tacaccatgt
     7981 gtacacgtgc gatgcatgaa ttgacccttt ttacctccaa taatgaaagt cctttgttaa
     8041 acaatatgga tatttcatct tatgagaaaa aagtactgta agtaaaaaga caactgctat
     8101 cattaatgtt agcagttgtc tttttattta caataataat tgcagaaaaa gtaattcaat
     8161 catttaaggt catttattct atatataaat ccaaatctgt ttcaggatat tttaatttga
     8221 caagcgaaat aatcattttc agttcttcca tgttttctgg atattctggg tcaaatacct
     8281 tgctatgacg gccaatcccg ccattgtcga tcaatcggta aattcgattc cattcaatga
     8341 attggtcaat tgattggaaa ttgctgattg gatagccgcc acaatccaaa atctctgata
     8401 cacgaaatgc taggtatgca ctatcgatca ctttattttt tttcgtgtct atcttctcat
     8461 ttctttcgat aagaattttt tcaggacgat gtttcaagct ccactctaat agtagaaaga
     8521 aagcctccat ccgatactct tcttcaaata accgaaaggc tgaatcagaa cgacgaaaaa
     8581 gcagcggaat cagataattt tgtctataaa tcagcattgg tgcgcggtcc accatacact
     8641 ctaaataacg gttcagatac cctgtaccga catagtttcc taatcgatag ccttcaacgc
     8701 cgaatcttgc gtatttttgc gctaattttg aacaatcagt tacgttcaca gctaactctt
     8761 ctactttgcg gtaaccgctt aagatacgtt caaaaggttc taaagtgatt tttgcttcca
     8821 tattcttccc cgtccatctt ccacatactt catatcattt cacatgatac ttatgctctc
     8881 atcataccac aggttgaatt agaaaagaaa ataaatcaca caaaaaagca agacaatctg
     8941 ttagtgagat tgtcttgctt ttcgaaaaag actgaatact tttcgttaaa actggttgct
     9001 tagtcttcta atgccacatt atggaacaca tgctgtacat cttctagatc ttccagtgca
     9061 tcaatcattt tttcaaatgt ttcttgatct tctggagata gtgttacctc gttttgaggg
     9121 atcatttgga tctcagcgat tgaaaaatca gtgattcctt tttcttttaa tgcttcttgt
     9181 atactatgga aatccgctac ttcaccgtaa acgatgattt gtccttcttc ttccattaca
     9241 tcgcgaacat cgatgtcttt ttccattagg tattcaagga tctcgtcagc gtcttctcct
     9301 gcaaaagcaa agattgctgt attgtcaaac atgtaagaaa ccgctccgga aacacccatg
     9361 ttcccgccat ttttcccaaa tgctgcccgt acgtctgctg ctgttcgatt cacattgttc
     9421 gtcaatgtat caacaatcac cattgagccg cttggtccaa atccttcata gcgcaattca
     9481 gagtatgttt catctcctgc accttttgct ttttcgattg ctcggtcaat aatatgtttt
     9541 ggtacattat aagttttagc gcgatcaatg acgaaacgta gtttttggtt tgcatgcgga
     9601 tcaggatcac ctgattttgc tgccgcgtag atttcaatcc caaattttgc ataaatccgg
     9661 ctattattag catctttagc agttttcttt gctacgatat ttgcccattt acgtcccata
     9721 ttttcacttc gctttctgat ttgatagttt cataacgcaa aaacgtcctg ttccattata
     9781 cttgtgaatc acgattctgt gaagcttttt ttctttcttc cttcattctt ctcattttta
     9841 gtcaattatc tatactcgga ttgggatttg cccgaaattc tgttccggaa tattgcctcc
     9901 caattgatag atttgatcac ttctaactaa cagttccgct tcttgtgcct ctttttttcc
     9961 gattcgggaa taaatagaaa tctctttttt ttcttttaag aatcttcttc cagcatcggt
    10021 aaatccaagt agtcgaagat aatttttatt ttgttggaca ggaatctctt gctttttgat
    10081 attcagcaag acataacaag ataatcgttg gatccgtgtc catgtatatc tttttgtttt
    10141 tacctttgac acaaaatcct caaaactatc agcagttgca gctgctgttt tcatgcgcga
    10201 ttctattcct tcggtcatct gatagatatc tgtcagttcc agcaaagtag aagataacaa
    10261 ccgataacgc aaatacggcc aataagattc ccaagtctgc agcgaacaag ttgaaagatc
    10321 ttcttctgtc tgaggcggca caagtccagc tattcttttt ccttcttcta acgcatggcg
    10381 aatggctgtt gcactggcta tcctctgatc tgtgatgatt ttttcatgat agcctgcact
    10441 tttacgtcgg atcggatgta attccatagg gtggacatac ttggcatttt cctttgcata
    10501 acttaatccc aagatatgat ttggcgaaga aaaatcaagc gtaatatcgg gatacagact
    10561 gcgaaaaacc tcagtcattt tttgcggata actatacgtt tgatcagaca gttcttgaaa
    10621 tgcctcttct atcctatctt gattctcttg gacaaagcgt ccgaaactgc tgtaatcaaa
    10681 cgaagtctct tcttctgttc caaaacacaa actgtcacag tccagtgttt gcaagatttt
    10741 tactgcacct ttagcaaaat agtcagctga ctgaaccgcc cattcgacgg gaagctccac
    10801 tactaaatcc acaccatttt gtaaagcttc attcgcgcga tgccatttat cgatgatagc
    10861 cggttcgcct ctttgcaaaa aattcccgct cattacagca atgacgacct ctgctcctgt
    10921 tatttttcgt gccatttcca catgatagcg atgtccattg tgaaaaggat tgtactctac
    10981 gataatcccg cagcttttca tgcgcatcct ccttcatagt tatcgataaa aaagaaatag
    11041 agacaagtaa aaaaaggcaa aaatcggaaa aaatacccga actatataaa ctattctcgc
    11101 aaacacaata cttctgcgac gaaaagctgc actgcaagag caatgcctat atcgttcgga
    11161 tatataattt atgttgaaga tttattcttt tcttgttaac tcgagcaggc aaaaccgcta
    11221 gtctttgtat gctacaaaga accagcgctt tgatttctct gatgctgctt cgtctgtaaa
    11281 atcagaaaat gcttcgactt tcgaaaagcc gctgttttcc agcattctta aataactttc
    11341 caatggatag gtacgctctt gatgtagttc atcttcacga ataaatcgac cagacccttc
    11401 ttgttcttca acgaaaaagg taagaaaatg ttcgatgctg tgtttctcgc ttcctggata
    11461 actatcccac aaaaaagcaa aattatcccc ttgataatga tagctatatt ctggaaaaac
    11521 ctcatctact tgataggtgg agtgtacgtc aaataaaaat cggccatttt cttctaacgc
    11581 ttgatagact tcatcaaaca cttgctgtac ctcttgtcta tttttcatat agcacagcga
    11641 atcagaaaaa caggtaattg cctgatattg tccgatttct gataaatcga gcatattgcc
    11701 ttccacaaat tggatcgatg cgtcttcttc atatgctcgc tggctggcaa tcatcaacat
    11761 ctcttctgaa aggtctaacc ctgtcacttt atatccatct tttgcaaagg caaccgctaa
    11821 tgctccagta ccgcatgcca gttccaaaat attttttgtg tcttcttgta aatgacgttt
    11881 cgaaaaagct aaccattgct gataaagtgt ttcgtccatc acttcatcgt aaatgaaagc
    11941 aaatgtttca taagccatat gttaatccaa ccattcacta agatcgacta gcggagcatc
    12001 tgaccatagt ttttccagat tatagaaatt tcgttctggc gcatggaaaa catgtacgat
    12061 cacatctcca agatcgatca atacccattt tccgccttct tttccttcaa tacgttttac
    12121 ttcatatttg ttttcttctt ctttatcaat gatttcttct gtaattgcgt tgatttgacg
    12181 ttcgctattt gctgaacaga tcatgaaata atctgccaac aaggaaacat ttcgaacgtc
    12241 taaagcgata atgtcttctg cccgttttga atctgctgct tcaatcgcaa tttttaattg
    12301 ttctttacta tctatgatga tttcctccta atatgagcag cttatttttt tgctacccag
    12361 taattatatg tttcgattgt ttttggataa atctgttgtt cttgcgcaag caaatgggcc
    12421 aatgtatgtt ttgtttcaaa tgctacagct tcgtctagat cagcccatgc aatggctcgt
    12481 gcatcttgta ccccaggaaa atctcgacct ggttcaatat agtctgctac ataaatgatt
    12541 ttgtcaagga gactcatctt agcagcccca gtcgtatgca cccggattgc atgaagaatc
    12601 tctgcatcgg tgattcctaa ttcccgctct acaaacgaag cgccaacaag accatgccag
    12661 attgcattat tataattcaa taactccgga tcaaaaccgt ctcttacgat taccattttg
    12721 aactcctcat ctggtcgttc ttttgcataa tcatgtgtca atgcagcaat actagctttt
    12781 tcaggagaag caccgtattt tttggcaaga gccacggctg tttcttctac tccaagcaca
    12841 tgcttgaagc gttgttcgct cattctcatc tgtatttttt gcatcaggtt ttctctagaa
    12901 taagcagtat attcattact atagtccata ttcatacaac ccttcatttt gaatataatc
    12961 aattacttta tctggaacaa ggtagcggat agaacagcct tctttgatct tttgacgtat
    13021 tttcgtggaa ctgatgtcga tctcaggtac atctacccaa attacaggat atggagtatc
    13081 cgttgtgtat cctggtcgac gtatacccac aaagttgacc atagaagtca gctcatcgat
    13141 tttataccat tttggcaaat attctaccat atccccaccg ataataaagt aataatctgt
    13201 atccggattg ttttgcgtca attccttcat agtatcatag gtgtaacttt tcccaccacg
    13261 agctaattca atcgtttcaa tctgcaaaaa aggattgtct tccaccgcta attccaacat
    13321 gttgagtcga tgttttgcat caatcgtctg tttctcatca acatgaggtg gctgataaga
    13381 aggcatcaaa aatacgcggt caagacctaa attccggcca gcttgttcag ccatcactaa
    13441 atgtgcaagg tgaacgggat tgaatgttcc gcccaagatc cctacttgct ttttcttctc
    13501 taagaattga ggcatttctt caggaaataa ttgtgaacga acaaatgtct tagcttgcgt
    13561 attcatcatg gtcactcctt atattgcttt tacttctttt gatagccttt gatacttttc
    13621 ttttgtggaa ggtttgtata atacaataac acgtccaatg atttggacga tttcgcaatc
    13681 tatttctttt tctaatactt gagctgcttc ttctgcaatc tcatctgtat tttgaagtaa
    13741 cgatactttg atcagttctc ttttttctaa agcttcttca atttgaacga tcattgctga
    13801 attgatccca ccttttccga tttgaaaaat cggctgaaga tgatgcgctt tgctacgtaa
    13861 aaaacgcttt tgttttcctc ttaaactcat aattttcctt cttccttaaa tcaatgcttt
    13921 gcgtttcaat acatctacac cctttggcgc ccagcccgca attactactg gcgctgttac
    13981 agtgatccag cctagaccgg caaaaacgat gtctgttttt tctttcacag aaaactcaaa
    14041 acgaacaagc tccggaaatg cctcgacttc atcaggacga ggaggctgta ataatccacc
    14101 tacatgtttt tgataaaatt cgtctgcttt ttccagtttc gttcgatgaa tatttacatc
    14161 atttgacaca taagcaataa atgaagcacg attgccgctg ataaaatcaa atcttgcaag
    14221 tccgcccaag aaaagtgttt gcccttcatt cagctgataa actttaggtt tgatttcttt
    14281 taacggtgca atgatccgca aatctttttt acctaagtag tgcgccattt gatggcgatg
    14341 gataattccc ggtgtatcaa tcaaaaaatg cccgtcatct aaaggaatct cgattttatc
    14401 caaggtcgtt cctggaaact gcgaagttgt gattacatct tgtactcccg ctgtattttt
    14461 gatgatttga ttgataagcg tcgattttcc gacatttgtt actccgacga tatagacatc
    14521 ccgtccatcg cggtattctt cgattttttc taatagttcc gtcatctcat gagatttctt
    14581 agcactagtt aataatacat ctactggacg aattcctgct tcgtaagcac gttccttcat
    14641 ccattgtgtt aatttagttt ttttcaatga tttaggtaat atatccactt tgttcccaac
    14701 taaaagtact ggattgtcac cgacaaaacg gtgtagtcct gggattagtg aaccattgaa
    14761 gtcaaaaata tccaccacat tgacgatcaa tgcatctttt tgccctaatt cattcagtaa
    14821 gcgcaaaaag tcgtcatccg tcaatgagac atcttgggtc tcattataat ggcgtaatct
    14881 gaaacaacgc tgacaataga cttccccgga agctaaacct ttttccaaag ccgtttgcgg
    14941 cgtgtatcct aattcaccgg gtttgtttgt ttgtataacg gcaccacagc cgatacattg
    15001 taattcttct gtcattcgat tccacctcgc cagcccatat cagggtgttt tgctaataga
    15061 taggccataa ttcttttttc ccaaaaacga ttaaattgtg tcttccaact atctgttgcg
    15121 ataatgggtt ttacaaggat gctacgtatc cctgcacgat tggctccacg tatatcagtc
    15181 ataatctgat cgcctaccat tactatctca tcatctgcta aatccaattg tttctttgct
    15241 atgttgatcc ctcttgctaa aggtttcatt gctcgtgcaa catacaaaag atcaaatttt
    15301 tcaatcgctc gcgcgacacg ttttgaatta ttgtttgaaa caacaacaac agggattcct
    15361 gcatttttca tttctaaaat ccaagtaagc aactcttctg ttccatctgg attattccat
    15421 gcaatcaacg tattgtctaa atcggtcaat actgctttga tgcctagatt ctttaattgg
    15481 gcaggtgtaa tctgatagat ggcttctacc atccatgttg gtttgtaatt tgaaaacatg
    15541 ctgtctcctc atctgcaaaa aatgaaggag cacataaagt actcctcaac cccgtattat
    15601 actgcatatc agcaaaaaaa actacctcta tactcgttcg tctttcagaa aaagcatttc
    15661 tttacaaaat gaccaaaaat aaagattgat cacagaaaaa tttagaaaca aaaaacaact
    15721 aatattggcg gaacctcttg tatcgttttt ccagtaattt atgctgatct aatcgaacaa
    15781 gttccgtcaa tctttcaatc aacgcttttt tcataatgtg tgcgacttta tatcgtggca
    15841 actgctcgcc gttgaacgtt tcaggaataa ctcgatcaac gattcctaac tcgcgtagtt
    15901 ctggcgcagt aattttcatc aactcagctg cttctgcggc gcgactaccg tctttccaca
    15961 aaatcgacgc aaatccttct ggagaaagca cagcataaat cgtttcttcc agcatccaga
    16021 cttcatccgc taaacctaga gcaagggcac cgccacttcc accttcacca atgatgatgg
    16081 cgatgatcgg tacctttaaa tcagacattt cgagcagatt tctggcaata gcctctcctt
    16141 cgccgcgctc ttctgctcct acaccgcaat atgcacctgc tgtattgatc aaggtaatca
    16201 ccggtctatt gaacttttcc gcttgtttca tcagtcgtaa agcttttcga tagccttcag
    16261 gatggggaga accaaaattt ctgcggatat tttcctgcag atcccgtcct ttttgaatac
    16321 cgatcactgt taccggtttg ccatctaatc gagcgattcc accaacaatc gcttcgtcat
    16381 ctgcaaataa acgatcacca tggcattcct cgaaagaatc gaagatctct tcaaaaaaat
    16441 ccaagcttgt ccatcgttct ttagctcttg ctaactgaac gacatcatgt gctgtcttat
    16501 ccacgatcta tccatccttt ctgtgtatgc aattcaagaa gcgtatggat tttctgtttc
    16561 aattccatgc ggggaacaat ctgatcgaca aaaccatgag ataataagaa ttcagccttt
    16621 tggaaatctt ctggaagttc ctgcttgatc gtctgttcga tgacgcgtct tccagcaaaa
    16681 ccgatcaagc tttggggttc tgctagaata atatcgcctt ccattgcaaa actggcagtt
    16741 actcctccgg tcgtcgggtc cgttaaaaca gttagataaa aaagaccttc attgctatgc
    16801 cgtttgacag cagcagagat ttttgccatc tgcattagag aaaagattcc ttcttgcatc
    16861 cgtgcgccac ctgaagctgt gaacaagacg actggaagac tttcctttgt tgctcgttcg
    16921 aataatctgg tgatcttttc tccgacaatc gttcccatac ttcccatgat aaaattagga
    16981 tccatcactc caataatgaa gggaatcccc tgaatggttg ctttaccagt gaggactgct
    17041 tcatgaagtc ctgttttatc ttgcattttt ttaatttttt ccgtatattc gggaaatgct
    17101 agcggatctt tggttacaag atccgtatcc cattcttcaa aacttttttc atcaataatc
    17161 aaagctagac gctgccatgc accgattcga aaagcgtatc cgcaatgagg gcatactttt
    17221 tcttcaccga tgtctttcgt ataaaggata tgtttgcaat ttggacactt tgcccacatg
    17281 ttatcaggaa cagatggcga gttagcaggt ttgctctctc tattaggatt gatccgaata
    17341 tatttctttt tcttaaacag agccatctat gcgcctccta tctccgtttc tggtgtccaa
    17401 ttcggtaaaa attcttcttg taaaaatgct gtgctgtaat cacctgctat gactgcagga
    17461 tgactaatca aatccatctg aaattctgcg ttcgtgatga ttccttctgt gataaattca
    17521 cttaaggctc gctgcatctt cattaatgct tcaaaacgcg tatttccatg gacgattatt
    17581 tttgcaatca ttgaatcata atatggcggg atcgtatatc ccgggtaaac tgcactgtct
    17641 actcgcagtc ccatcccacc tgctggaagc agtaagtttt ggatcttccc tggagaagga
    17701 gcaaaatgga aagcaggatt ttctgcatta atccggcatt cgattgcgtg tccttggaat
    17761 actacttctt cttgacacac attcaaaggc tcaccggctg ctatctccac ctgttttttt
    17821 actaaatcga ttcctgtgac catttctgtt actggatgtt ccacttggat tcgagtattc
    17881 atttccatga aataaaaatc gccttcttca tccatcagaa actcgatcgt tccggcattt
    17941 tcataattga ctgctttagc tgctcgaaca gcagcgtctc ctagtgcttc tctttttgtt
    18001 tgtgagatca ctacagaagg agattcttcc agtacttttt gattgttgcg ttgtaaagaa
    18061 caatctcgct cgcctaaatg aatgacatga ccaaatcgat cacctaagat ctgaacttct
    18121 atatggcgag caggataaat gattttttcc aaatacatat catcatttcc aaatgccgct
    18181 gctgcttctt gctgagctga agaaaaatgc tgtgggagct cttcttttga ttgaactttc
    18241 cgaatgcctt tcccgccacc acctgcagct gctttcagca tcactggata accaattctg
    18301 tctgctattt ccaatgcttc ttctactgta gataatacac cagtacttcc tggaatgact
    18361 gggacatttg cttcttgcat caattgacgc gcattgatct tatttcccat tgcatcgatc
    18421 gttgcacttt taggaccaat aaatgtgata ttgcactctt cacacatgga ggcaaatcgg
    18481 ctattttcag ataaaaaacc aaatcctgga tgaattgctt cggcttttgt cacgattgca
    18541 gcgctcagta cttcctgaac attcagatat gaatcaacgg cttttgccgg gccaatgcag
    18601 attgcttcat ctgctaactg tgtatgaagt gcttcggcat ctgcttctga atatatcgct
    18661 acggtttgga tccctagttc gcggcatgca cgaatgatac gtacagcgat ttcaccgcgg
    18721 ttagctatca atactttaga aaacatattt cacctatcca atcataaagg ttaattccgc
    18781 ttcggctact tttttaccgt ctactttagc gattcctttg cctattccgg ctgaagcttt
    18841 cactttcaat atctcaactt caaggtataa ggtatctccc ggtgtaactt tcttacggaa
    18901 ttttgctttg tccaatccgc cgaaataagc cgtctttccc ttgaattgtt caagagaaag
    18961 taatgcaact gctcctgctt gagccattgc ttcaacaatc aatacccctg gcataacagg
    19021 ttcttctgga aaatgtcctt gaaaaaaggg ctcattgatc gttacatttt tcttagcaac
    19081 gatccgttct ccttcaacca tttcttccac tcgatcaatc aaaagcattg gataacgatg
    19141 tggaatgatt tcttttattt cttgtatgtt cattgttttc cctctttcct gctttgctta
    19201 tttttgctga atcctaaaga gtggctgtcc atattcgaca actgactcgt tttctacaag
    19261 gatttctgtg atcactccgt cggcctcact agttatctca ttcatcagtt tcatcgcttc
    19321 aataatgcag ataacttctc ctttggcaac ttgatcacct acttttttga aagctggttt
    19381 gtcgggcgct ggttgcagat aaacgattcc cacgattggc gaaacaacct cgattccatc
    19441 aaatactgcc gattcttttt ctttcattat ttctggagca gttgttgctt gttcaggtat
    19501 gacattgact ggctccgaaa ctggcaacgt ttcgtttgtt tgaaccatat tcatgcctcg
    19561 acttgtttta tttttattca tatacaattc aaattgacct tcacgcaaat cgaattccgt
    19621 tagcgaggat tgatcaaatt gactgactaa ttctctgatt tcattgatat ccaaaattat
    19681 tcctcccagc gtttcatgca cagaaccgca ttatgtccgc caaaccctaa tgaattactc
    19741 attgcataag taaaacttgc aggtttgctt tcgtttttga caactgtcaa gtcaatctca
    19801 ggatcttgtt tttctacatt gatcgtaggt gggataaatt gatgttctaa tgccagcaaa
    19861 gttgccaagg cttcgatccc gccggctgct cccaataaat gtccagtcat cgatttcgtg
    19921 ctgctgacgt aagtatctct gaaattttca cctaatgcga tttggattgc ttttgcttct
    19981 gcctggtcat tggctggtgt acttgttccg tgagcattga tatatccgac ttgttccggc
    20041 tgtacaccag cttcttccat cgcaagcttc attgctttag atgcaccaga accatctgga
    20101 gtaggagctg tcatatggta agcgtcacag tttgcaccgt atccaacgat ttctccatag
    20161 attttggcac cgcgagcttg tgcatgttct aaagattcaa gaactagaac gcctgctcct
    20221 tcacccatca caaaaccatt ccgattttcg tcaaacggaa tcgaaccacg attaggatca
    20281 gttgattctg agagtgctgt taaagctgca aatccagcaa tccccatctc acaaatcgtt
    20341 gcttctgttc ctccagcaag aatgacatca gaatagccat gtttgatgtt acggaatgct
    20401 tccccaattg aattcgttcc gctagcacaa gctgttacga tagctgtaca aatacctttt
    20461 gcacctacac gcaaagcaat atttccagct gccatgttgg caatcgccat tggtacgaac
    20521 attggagaaa ctcgttttgc acctttttca tgtagacgaa taacttggtt ttcgattgtt
    20581 ggaagaccac cgatcccaga tccgatgatg acgccaaacc ggtcttgatt cattttttct
    20641 ttatctaaac cgctcatctc cattgcttct aaagcagcgt agattccata gatcgaaaac
    20701 atatccattc gtttgctatc ttttttaata aaatatttat cgtaagggaa atctttgact
    20761 tcacccgcta aggtaatccc tgtttctgaa gcatcaaatt tagtgatcgg tgcgatgcca
    20821 ttcgtcccct cttttaagct attcaaaaag ctgtctgcgt catttccaat aggtgaagtg
    20881 actccatagc cagtaattac cactcgattc atttgtttgc tctccttttc ttatccgtgc
    20941 atgaccaatc cgccatcgac attgatgact tgtccggtga tatatggatt ttgtgctaag
    21001 aaaactgctg tttgtgcaat atcttctact tgtccgaaac gctgcatcgg aatttgcttt
    21061 tccgcttgtt ccttgacttt gtctgctaaa acttccgtca tatcagttgt gatgaatcct
    21121 ggcgcaatgg cattacatgt aatgcctcta gttgcagctt ctcgtgccac agattttgtt
    21181 aatcctacta cgccagcttt actagctgca taatttgctt gaccaatatt tccaatcaac
    21241 ccagatacac tagagagatt aatgatggcc ccttctcgct gcttcatcat ttttttcaat
    21301 acgtgctgcg tcatattgaa ggttccaatc aaattgatat ccagacattt tttgaaatct
    21361 tctgcatcca ttcgcatcac aagtttgtca tttgtgattc cggcattgtt tactaacaca
    21421 tggatcgatc caagcttttc ttctgcttct ttgatcattt gtcccgcttt ctcataatca
    21481 gagatgtcac cggaaactcc tacgcatttt acaccgaatg cttcgatttc ttctatcttt
    21541 tcttttggaa tctctccgcg accattcaaa atgatatttg ctccagcttt tgcaaaagct
    21601 aaggccatcg ctttgccgat tcctcttgta ctacctgtga taaatacatt ttttcctttt
    21661 acatccataa cgttcctcct tatagtattg ctattgtttc atcaaatgtc tgcttattct
    21721 caacacgcag aactggtatt gttttatcga tttttttcat aaatccgctt aatacttttc
    21781 cggggccgac ttcgatgact tgttcgatgc cgattgtttt taacttatgg atactctcat
    21841 agaaacgaac aggttgcatg acttgctgtt caagtagtcc cgcgattgtt tctttcttca
    21901 ttatttccgt tgttgtgttg cttatgatag gaaaagaagg ttcagagaat tgaatctgac
    21961 ttaggtcctt cgccagtttt tttgctgccg gttctaaaat agctgtatga aaaggcccgc
    22021 tgacatttag agggatcatt cttttgaacc ctttttcttt taatagtaaa acagcttcgt
    22081 ctactgcttt ttcttcgcca ccaatcacga tctgctgagg tgtattataa tttgcaggag
    22141 atacgatgcc gtacttgcta gcttcatgac agctttcctc gatcgtttca atcggcgcat
    22201 tcatcactgc aaccattttc ccgcttccag ctggggcagc ttctgtcata taagcgcctc
    22261 tttttgctac taacgcaact gcttcagtga aggatagcgc attactagct actaatgccg
    22321 aatattctcc taaactcagt cctaaagctg catctgggat gattccatgc tccttcaata
    22381 agcgataata cgcgatgctg actgttagaa tagctggctg tgtatattgc gtctgatcca
    22441 aacgttcatt ttctgtaaag caaagttcag ccatatcgta gccgagaatc tcacttgctt
    22501 catcaaatgt ctgctttaca acggcttctt catataaatc ttttcccata ccttgatatt
    22561 gtgcaccttg gccactaaat aagaatgctg ttttcatgtt tttctccaat atatccagaa
    22621 tatatgtttg attcttccta ttcaaaaagg ccgagacatt cctatttcag gaaatgtccc
    22681 agcccccaac ctaattttaa tgattatgca gtttgttttt ctacgtaagt cactaaatct
    22741 tgaaccgttt tgattccgtc ttctgtttcg attttcacgt caaattcatc ttcaatttca
    22801 ttgatgattt ggaataaatc caagctgtca gcttctaagt cttcttggat gtcagtagtc
    22861 aatttgattt cttcagtttc ttttcctaat tcttctgcga tgatttcttg aattttttcg
    22921 aataccataa taatatattc ctccgatatt gtttgctttt tatttttata atttaacagt
    22981 ctacaactcc atgagaatac ttccccatgt taaacctccg ccatagcctg taaatattac
    23041 tttctgctgt tttcctaagg tgatctttcc actagaaact gcttcatcca ataagatagg
    23101 gatgcttgca gcagatgtat tcccatattt atccatattg gtcaggaact tttcttgcgg
    23161 catctttact tttcgggcaa ttttctcaat aattctgagg tttgcctgat gcaaaagcag
    23221 ataatcaact ggtttgtctt tcatgacttc ttggatattg tttgcaacgt ctcgtacggc
    23281 aaaatcaaaa atatctctgc cagtcatttg caaataatac gaaccttctg aatcttctga
    23341 atagaacgga tttttatttt gctggtagcc agcggtcaat gagctacttc tggtaccgtc
    23401 agctgctaat ttttctttca gcaatttttt ctgtggattc gcttctaaga gaatcccacc
    23461 tgctccatcg ccaaacaaca cagcagtcgt acgatcagac caatcgatta gcttagatag
    23521 tgtttcacca ccaatcacca gtcccttagt tgagccgcag cggatcattt tttcagccat
    23581 gcttaacgca taaacaaatc ctgagcacgc agcactgata tcaaaagcga tagcatgagt
    23641 tgctcccaat ttcccttgta cttgtgctgc tacagatggt gtcgtgtaat cgggcgacat
    23701 tgttgctact aaaataaaat ccaaatcttt agggtttaga tttttctgtt ttatcaattt
    23761 ttccgccact aaaatgcata aatcagaagt ctcctgattt gttacacatc ttcgttcacg
    23821 aatacccgtc cttgacgaaa tccattcatc agaggtatcc atcatcttgg aaagatcatt
    23881 attagtaata atcttttcag gaaggacgct ggctgttgca gtaatcattc caaattgttc
    23941 cattaaatat cctcacttat agtcttgcaa gaagtcgtgc aagtttttca gtgcatccag
    24001 caaggcatgc tgttcctccg tagccattcc gtctaagatg ttcttgacca tttcacgatg
    24061 aaaatgctga tggacccgaa atagtaactt tccttttttc gttagtccca actttacaac
    24121 gcggcggtca tcttcactgc ggatacgttc gacatagcct ttttttacta acttgttaat
    24181 agcagtggtc aaggtcccaa cagtaattga tagttctttg gctacttctg aagttgtttt
    24241 tttcttgtac atcccgattg cctcgatcgt gtgcatctct gtgattgaca gatctttgaa
    24301 ttgagatttt ttcagctcag actcttcgat tgtcaaaata tcattgaaga cactaacaag
    24361 ataatcattt actgttttta aatctggttc catgttccac cgtccactaa ttagtttgat
    24421 tatcaaatct tttgatgttc aaactatatc ttaacttttt tcaaaatgca accttaaaag
    24481 atcatttttt tactatatga gcttttttta agattttact catttaaaac aactgataaa
    24541 tcagcattta taatttcttt tattagaaaa atcatctatc tcacatattt tgattatcaa
    24601 agtattttct gtgaacagaa ctatagaatt ccctaaaact tgtccattat ttcaaaattt
    24661 gttatagtct ttttattcaa agcgtaaaag gagatcctat gttaaaagaa aaaatgaaaa
    24721 ctctccccat gctgccgggt gtctatctta tgaaagaccg agatggtaag gtcatctatg
    24781 ttggtaaagc aaaacggctc aaaaatagag taagcagcta ttttcatcag aataaacagc
    24841 attcaaaaaa agtattacgt atgattcatc atataacaga ttttgatttc gtcgtagtag
    24901 acacggaatt agatgcttta ttgctcgagt gtcaattgat ccaacattac cgtccttttt
    24961 ataaccgaca aatgaattat ttcagtaatt ataattatgt acatattacc aataagggat
    25021 ttgtattaac agatactcct acggctcgaa catacgggcc ttttcgtctc tataaaaaaa
    25081 tgccgtctat tttgcgtata atggaagaaa cttatcaaat gccttggttg tctgaaatta
    25141 gtttgttagc tttgcgagtc cagcttcctg acctacagga aatgactttt gaacagaaaa
    25201 aaaaggaact ccaagggctg tttcaaggcc gaaataaaaa gctactgact tacttgaaaa
    25261 aaagacaaca acatttcatc tatcagttga atttcgaaaa agccggtatg cttcaaaaag
    25321 atatcgaatt agttacttat tttattagac ggatccagga acaaaagcag tttttaagaa
    25381 caccatcgct caccttcagc atgccattag cagcagatga gagtcagaaa aagcactacc
    25441 tcgtttgtta tggtcaactt gcagaaacaa tgattacctc tggtgacgtt tctcctgact
    25501 tctattatga aaagaaagaa gctcatttat ctttgaaacg tcaattgtca aaagaagaaa
    25561 tcgatcctgt acaaatcttg atcagttacc gcaaaaaatt agaaaaagaa cagtttgaaa
    25621 ttgaactgtt aaacaaaaaa gaagctgaaa agcaactaaa ttagttgctt ctcggcttct
    25681 tttaggcatc ttcaacttca aatgtcaatt atcctaaaat ttattggatt aagtcataga
    25741 tttccatcgc tacgatatcg atattatcaa attcgtattg ctgatgagta tctgtttcag
    25801 ttaattcaaa tgtatcggtc gctttatcat aagttactgt acacttctct acgccttctt
    25861 tttcaaaacg gcgaacttgc agttcattat ctgctgactc gaccatagcc tccagtcgtt
    25921 ttatgattgc gactaattgt gaatgtgtca tcggaggtcc tccttttctt atttctgtta
    25981 tattgtatca aaagcatagc ctacttgcat tgattttctc taaaaaaact cgttttttat
    26041 gatttttttt ctggtatttt tccttcaggc caccaaagtt tgatcaaagc ttgggttccg
    26101 tcattctcaa aacctttatc tgccaactct tcatatagtt tcaatgcttg ggtagttgct
    26161 ggcaatacaa gatccatttt ttctgcttct tctaatgcaa tctttaaatc tttgataaaa
    26221 tgtttgacaa aaaaaccagg tgtgtaatct tcttttaaaa tacgaggaga ataattgctt
    26281 aaagaccagt tagctgcact tccacctgaa agcgtctcaa tcactttctt taaatcaagt
    26341 ccgttttttt gtgcatagac aagcatttcc gtcattcctg tcatcgtacc tgcaatcatg
    26401 atctgattag ccatttttgt atgctgacct ttcccagcag aaccatgcaa tgtaaatgtc
    26461 gtaccaaatt ctttgaataa aggcagtact tgatcataaa cggcttcttc tccaccgacc
    26521 atgatcgtca gtgtaccatt ctttgctcct aagtcaccac cggataccgg tgcatctaac
    26581 gcatctgcgc ctttttcttt tgctgttttt gcaatttttt ctgccaggct tggtgtactt
    26641 gtagtcaaat caactaaaat atgtcctgag atatctgcag aaaaaatccc cgactgtcca
    26701 tagtagattt cttctacatc tttggggtaa cctaccattg taaagagaat attactttgt
    26761 tctgctaccg cttttggtgt atctgcccag atagctcctt ctgttactaa aggatcagct
    26821 tttgcttttg tccgattata gacagctact tcgtgtcctg cttctagtag atggcgtgcg
    26881 acagcacttc ccatgacgcc tgttccgata aaaccaagtt tcattttttc gacctcctaa
    26941 aaatcaaaaa tccttgcttc tatgctagta tacgctagaa acaaggattt tttcataaaa
    27001 aatgtttgaa ttttatcttt taaatgaatt taattttatt ttgattcgtt caatcgagtc
    27061 ttcttcttct tttcttcgca tctcttcttc atgataaaca accatgtctt gataaaattc
    27121 gatcatagct tcgccgaaat gtgtagatga aatatcataa agtttagctt ctaaaacttt
    27181 tggatcttta ggaacttcac tggaaatata atcaataagt gctggtgcaa agtcttcgtc
    27241 ggtttcaaaa gttttcccta atgaaggatg atcgaacaat cggttcaaat aagcatttcc
    27301 ttccacgaca caaggtgttc cggctgccat cgcttctgta taagtaagcc cttgtgtttc
    27361 tgaagtagaa gcactgacaa aataatctgc cgctttataa taaagtgcta catcattgtt
    27421 gtccacttct cctgtgaact gcacaaattc ttctaactgc agttctgaga tcaattcctc
    27481 taactcttct acataaggtc cttttcctac gataactaat ctggcgttag gataagcgga
    27541 gatgacctga ggaaacccat taatcaaggc ttggatattt ttctcataag aaatcctgct
    27601 taaagaaaga atcatcaaat ggttttcctg aagacctagt gactccctta atttgctgat
    27661 atcctgctga gtgatatcag aacgtttgaa tttttcaatg tcgataccag taggaatcac
    27721 gcgaagcggt gctattacac catagctgcg taatgtatca ataacacgtt cacttggaca
    27781 aactacacct gtcgtatgat ttgcaaataa tctagacaag tattttacat gggattgcct
    27841 gatcactttt cctttaccga tataatgaag gtagtcttca tacatcgtat ggtaagtatg
    27901 gatcagaggt attttcaact tcttagctac catcttccca agtatccctg ctccgaattc
    27961 tgtatgggta tgaataagat ctagttctag ttctttggca accaaataag cataccacat
    28021 gcctctaaca acaactcttc gatccttgaa agaaataaat ggtacacttg gcattcgaat
    28081 cacatctttt tccaacttat ccgcattagg atcggtcgtt gtaaaaatat agacttcatg
    28141 cccatgtctt tctaattctt gttttaatgt tttgatcgat gtagcaactc cgctgacttg
    28201 cggaaagtag gtatcggtaa aaaagccgat tttcatgttt ttcgcctcca aactagtcct
    28261 tgacctgatt tacttttttt aatttgctag agactttttc attcagtaca ctttcgtata
    28321 catttttaag ttcttcaccg attttattga tacttcgctc ttttgctgtt tgataaccgg
    28381 cttgtgtcgt actcggtagt tcttgattgg taatctgttg gatcagtcgg ataaattctt
    28441 cgtttgtctt ccccatatag cagttcttgc tatcttctaa ccagccatta tacactggaa
    28501 tatctcgaac aagtacgttt tgtttgcttg ctaaagcttc taaaacaacg atcccttctg
    28561 tctcttcata agaagggaaa aagaatagat ctgcacctga atacgcacct tctatgatct
    28621 ctccacgaat gtaaccagga aacaggacat tttcaggatg atcttctttt acaattttcc
    28681 ggatgttccg aggaatcgag tacatgggaa catgaccaaa ccaaataaat gtgtattcgg
    28741 gcatcttctt agcaatttct acgaaatcaa tgattccttt acgttcgaaa aacaaaccga
    28801 cacaaatgat tactttttga tccggagaca gtttgaaata ttctctgaac ttttgttctt
    28861 tcattggatc agggtgatat ttctctaagt cgatcccatt ggaaatagct tgaatcggca
    28921 cacggatacc atagctttcc aacaactgtt ttgaataagg tgttggcgtg atcagatagt
    28981 ccgctttttg gtacaatcct accagatatt tttttacgat aggagaaagc tggttcgatc
    29041 cgataaagga atttcggaaa tcttcctctg tagaatgcgc atgatagaca actttttttc
    29101 cttctcttct ggctttattg accatattat gactattgat tccgtatgta ttgatgtgca
    29161 ggatgtcata atcttctttt tcatctagtg tataagagat accgacttcc gtcagagcac
    29221 gtttttgatg atcaagcgca cgcccgattc cagattttgc aagcatcttt tcactttcaa
    29281 aatataataa taccttcacc ttacgtcccc cttcacttat atagtataac atagcctaca
    29341 aactgtcact caaacttaag ccctat
//
LOCUS       DAJSBL010000031        28197 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.031, whole genome shotgun sequence.
ACCESSION   DAJSBL010000031 DAJSBL010000000
VERSION     DAJSBL010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28197
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.031"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            160..1302
                     /locus_tag="ONT85_001657"
     CDS             160..1302
                     /locus_tag="ONT85_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HCR2378634.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            1318..2529
                     /gene="thiI"
                     /locus_tag="ONT85_001658"
     CDS             1318..2529
                     /gene="thiI"
                     /locus_tag="ONT85_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HCR2378635.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
     gene            3102..3749
                     /locus_tag="ONT85_001659"
     CDS             3102..3749
                     /locus_tag="ONT85_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HCR2378636.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     misc_binding    3878..4094
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            4142..6787
                     /locus_tag="ONT85_001660"
     CDS             4142..6787
                     /locus_tag="ONT85_001660"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HCR2378637.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            7097..8410
                     /locus_tag="ONT85_001661"
     CDS             7097..8410
                     /locus_tag="ONT85_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HCR2378638.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            8400..9053
                     /locus_tag="ONT85_001662"
     CDS             8400..9053
                     /locus_tag="ONT85_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HCR2378639.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            9108..9659
                     /gene="radC"
                     /locus_tag="ONT85_001663"
                     /pseudo
     CDS             9108..9659
                     /gene="radC"
                     /locus_tag="ONT85_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
     gene            9790..10875
                     /locus_tag="ONT85_001664"
     CDS             9790..10875
                     /locus_tag="ONT85_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR2378640.1"
                     /translation="MKVQRIEVENKPYPLYLLLDKEYQLIEPVMKFIKYLDNTGKSPN
                     TIKAYCYHLKLLYEFMEQRGVILNDINFELLADFVGWLRYPSASNVIDLQSKKAIREE
                     TTVNTILNVVMSFLDYLSRLGEFKSIDVFKQAKGRNFKGFLHHVNKGRYQKNVLKLRV
                     KKKQIRTLRSEEVKQIIDACHTKRDKLILMLMYEGGLRIGEVLSLRLEDIVTWDNQIH
                     LTPRDVNVNEAYIKLRKERTIHVSKELMSLYTDYLIYEYSEELEHDYVFISLKEGYFG
                     KPLKYQSVLDLVRRIVKRTGIEFTSHMLRHTHATQLIREGWDVAFVQKRLGHAHVQTT
                     LNTYVHLSDQDMKNEFNKYLERKEHKK"
     gene            10872..12764
                     /locus_tag="ONT85_001665"
     CDS             10872..12764
                     /locus_tag="ONT85_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001833801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR2378641.1"
                     /translation="MNASSKRKIISQSEISKKIAVMNEEMQGFWANNSWDIRKCPHPS
                     AIELSKNPALRNRWVRFERVKNLWLRTELKYFYFYHLNNGIWNAKTVWIRKGTVINKM
                     LDFLDLKYPSITSITEVPIEKAMTEYRTYLTKRGVRITTTNYKITANQEKTPVKANSY
                     YVTNLKQFMEFYENFYFDGEEWDKDVWDRRNLPLPDDKVNPTQYEYTINFKGFRNTYF
                     KQLVKRYCKLRLNMDSFSYVSDIAQRLKEFFNFLDMKFKQVQRVHQLTRVEIEAYLSE
                     LNMMGIKPSTITGRISILEGLFSTLLRLEWDDVPSKILIYSEDYPKIPRAKPRFIDEF
                     VLEQLNSHLDKLPEYIATMTMIVQECGMRISELCTLKKGCLLEDKDGDFFLKYYQWKM
                     KKEHIVPISKEVALLIKVREDKVSEEFPDSEYLFPRKDGSPLKQETFRGELNKLAYEQ
                     NIVDKSGEIYRFHAHAFRHTVGTRMINNGMPQHIVQKFLGHESPEMTSRYAHIFDETL
                     KNEFTKFQEKLVTNNGDVLDLDEDNEVDDVELQWFKKNINAQVLPNGYCRLPVVAGGC
                     PHANACLDCTHFCTSKQFLPQHEEQLERTEELLAIAKDKQWQRQVETNSRVKERLEQI
                     IGSLTG"
     gene            12771..13148
                     /locus_tag="ONT85_001666"
     CDS             12771..13148
                     /locus_tag="ONT85_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR2378642.1"
                     /translation="MDKQVRNTTEIVRLAKQKSKKTREKVDKAISKFSIEGKVINFNS
                     IAKEANVSKSWLYKEHDIRQRIESLRERQITANVVSKPKKSSRSEEILIKTLKRRVME
                     LEKENKKLQNQIQKLYGDLYNKE"
     gene            13302..14084
                     /locus_tag="ONT85_001667"
     CDS             13302..14084
                     /locus_tag="ONT85_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(9)-Ia"
                     /protein_id="HCR2378643.1"
                     /translation="MSNLINGKIPNQAIQTLKIVKDLFGSSIVGVYLFGSAVNGGLRI
                     NSDVDVLVVVNHSLPQLTRKKLTERLMTISGKIGNTDSVRPLEVTVINRSEVVPWQYP
                     PKREFIYGEWLRGEFENGQIQEPSYDPDLAIVLAQARKNSISLFGPDSSSILVSVPLT
                     DIRRAIKDSLPELIEGIKGDERNVILTLARMWQTVTTGEITSKDVAAEWAIPLLPKEH
                     VTLLDIARKGYRGECDDKWEGLYSKVKALVKYMKNSIETSLN"
     gene            complement(14210..14941)
                     /gene="erm(A)"
                     /locus_tag="ONT85_001668"
     CDS             complement(14210..14941)
                     /gene="erm(A)"
                     /locus_tag="ONT85_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(A)"
                     /protein_id="HCR2378644.1"
                     /translation="MNQKNPKDTQNFITSKKHVKEILNHTNISKQDNVIEIGSGKGHF
                     TKELVKMSRSVTAIEIDGGLCQVTKEAVNPSENIKVIQTDILKFSFPKHINYKIYGNI
                     PYNISTDIVKRITFESQAKYSYLIVEKGFAKRLQNLQRALGLLLMVEMDIKMLKKVPP
                     LYFHPKPSVDSVLIVLERHQPLISKKDYKKYRSFVYKWVNREYRVLFTKNQFRQALKH
                     ANVTNINKLSKEQFLSIFNSYKLFH"
     gene            complement(14999..15058)
                     /locus_tag="ONT85_001669"
     CDS             complement(14999..15058)
                     /locus_tag="ONT85_001669"
                     /inference="COORDINATES: protein motif:HMM:NF033690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythromycin resistance leader peptide"
                     /protein_id="HCR2378645.1"
                     /translation="MGTFSIFVINKVRYQPNQN"
     gene            15450..16112
                     /locus_tag="ONT85_001670"
     CDS             15450..16112
                     /locus_tag="ONT85_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HCR2378646.1"
                     /translation="MNTIESQFDKVAEDYDFVNELLNDYSFFVSNMSPKKGRALDIGC
                     GSGLLVEKLASYYDEVVGIDISNQMLDLAKSKRQLTNTVYLNMNAEQLNFNEKFDFIV
                     SRTTFHHLDDIASVIQQMKELLNEEGRIVILDNVSEVETPPTYVYKLGAIQEFLPHCF
                     KFGIKNAIRIYNHNTSKSWLEHLASDKYLSEQNYYDLYEKLLPGCQFHKMGWAMGVVW
                     TK"
     gene            16347..16490
                     /locus_tag="ONT85_001671"
                     /pseudo
     CDS             16347..16490
                     /locus_tag="ONT85_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein"
     gene            16640..16840
                     /locus_tag="ONT85_001672"
     CDS             16640..16840
                     /locus_tag="ONT85_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HCR2378647.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(17094..18773)
                     /locus_tag="ONT85_001673"
     CDS             complement(17094..18773)
                     /locus_tag="ONT85_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HCR2378648.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(18775..18987)
                     /locus_tag="ONT85_001674"
     CDS             complement(18775..18987)
                     /locus_tag="ONT85_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HCR2378649.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            19380..21110
                     /gene="efrA"
                     /locus_tag="ONT85_001675"
     CDS             19380..21110
                     /gene="efrA"
                     /locus_tag="ONT85_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HCR2378650.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            21107..22867
                     /gene="efrB"
                     /locus_tag="ONT85_001676"
     CDS             21107..22867
                     /gene="efrB"
                     /locus_tag="ONT85_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HCR2378651.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            22962..23159
                     /locus_tag="ONT85_001677"
     CDS             22962..23159
                     /locus_tag="ONT85_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2378652.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            23312..23797
                     /locus_tag="ONT85_001678"
     CDS             23312..23797
                     /locus_tag="ONT85_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HCR2378653.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(23901..24494)
                     /gene="clpP"
                     /locus_tag="ONT85_001679"
     CDS             complement(23901..24494)
                     /gene="clpP"
                     /locus_tag="ONT85_001679"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HCR2378654.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            24674..25201
                     /locus_tag="ONT85_001680"
     CDS             24674..25201
                     /locus_tag="ONT85_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2378655.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            25292..26029
                     /locus_tag="ONT85_001681"
     CDS             25292..26029
                     /locus_tag="ONT85_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HCR2378656.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            26033..26950
                     /gene="miaA"
                     /locus_tag="ONT85_001682"
     CDS             26033..26950
                     /gene="miaA"
                     /locus_tag="ONT85_001682"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HCR2378657.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            26952..28181
                     /gene="hflX"
                     /locus_tag="ONT85_001683"
     CDS             26952..28181
                     /gene="hflX"
                     /locus_tag="ONT85_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HCR2378658.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
BASE COUNT         9420 a         4609 c         5934 g         8234 t
ORIGIN      
        1 aaaagttagt aaaccagcaa gagcaagacg gattattgtc ttgttcttgt ctttttttgt
       61 atatttgaaa attgaaacaa gtgaaaattt cacctgctct tgaaagcacc ttggacaacc
      121 gttataatag aaaagaattt caaaaaagag gggtttctta tgatatattt tgataacagt
      181 gcaacaaccc caatctttcc gcaaagctta gatgcatatg taaaaaccag tcagcggatt
      241 attgggaatc catcaagctt gcatgattta ggcaatcaag ctaaccggtt gttgcagcaa
      301 gcaagaaagc agatcgctga tttgatccat gtagaaccag aagaaatcta ttttacttca
      361 ggcggaacag aaggagataa ctgggtatta aaaggaacga tgatcgaaaa aagagaatat
      421 gggaatcata tgatcatttc aggagtcgaa catccggccg tcagcgagac agcagaacaa
      481 ttaaaagaat taggaatcga agtaagtatc gccccagtgg ataaacgcgg atttgtacgt
      541 gtcgacgaat tgaaagaatt aataagaaaa gagacagtat tagtctctgt tatggctgtg
      601 aataatgaag taggatcgat tcagcccatc caggaaatca gtgatctttt ggatgatttt
      661 cctaaaattc atttccatgt agatgctgtt caggcgattg gaaaagtacc attctcgcaa
      721 tggttgacag aaagagttga ttttgcaaca ttttcagctc ataaattcca tggcccacgg
      781 ggaactggtt tcatttattg gaaaaagggt cgtcgtttag caccattact gacaggtgga
      841 gggcaagaaa gaaatcagcg aagtgggaca gaaaatgttc caggtattgt ggctatggtt
      901 aaagctttac gtttatcatt agagataaag gaacaaaaac ctgagcagac acaacgctta
      961 aaagtagctc taatcaatgc gttgaagacg tatgagaaag tgacgatttt ttcagaagga
     1021 actgactatg caccgcatat tttatgtttt gctctaaaag gaattcgcgg cgaagttctc
     1081 gtccatgcct tagaagaaaa acaaatattt gtttctacta ctagtgcctg ctcctcacgt
     1141 aaacatatgg caagcagtac attgcacgca atgcatgtac caaatgatct ggctacatct
     1201 gctgttcgaa tcagcttaga tgaaaacaat tcgcttgcag aagtcgagca atttatgatt
     1261 attttcaagc agctttataa gaaattttct gcaatcaatt aaaagaggag tgttcttgtg
     1321 aaatacagtg aaattatggt ccgttatggg gaactttcga caaaaggaaa gaatcgaaaa
     1381 agctttatca tgcaactagc acaaaatgtc agacaagctt tgaaagactt tccagaaatc
     1441 aaaatccatg ctgatcggga ccggatgcat cttttattga atggcgcaga cgggcagcta
     1501 gttattccaa aactagcaaa aatatttggg atccaaaatt tctctccaag tattcgtgta
     1561 gaaaaagatg tcgaagtttt gaaacaagct gttcaagaca tcatgaaaga aatttatact
     1621 gggaaagaaa catttaaaat tatcgctaaa agaagcgatc accaatttga attgacaagt
     1681 aatgaattga atcaaacatt aggtaatgct gtttttgata ttttcccgca tatccaagtt
     1741 caaatgcgtc aaccagatat tccattacgt gtagagattc ggcgtgatgg cgcttatcta
     1801 tcgtatgaaa cgatcaaggg agctggagga ttgcccgtag gaacaagcgg aaaaggaatg
     1861 cttatgcttt ctggggggat tgattctcct gtagcagggt actttgccat gaagcgggga
     1921 gtggaaattg aagctgttca ttttgctagt ccgccgtata caagtgaaca agcactgcaa
     1981 aaagcaaaag atctaacagc caaactagca ccttatgtgg gaacgatcca ttttatcgaa
     2041 gtgccattta ccgagattca agaagaaatc aaaaagtcag tgccacaagg atattggatg
     2101 acaattactc gtcgaatgat gttgcgtttg accgatgaaa ttcgccgtat tcgtcatggt
     2161 ttagtcattt tgaatggtga atcattaggt caagtggctt cacaaacttt gcaaagtatg
     2221 gtggctatca atgaagtaac gaatacgcca atcattcggc ctgtagcgac aatggataaa
     2281 cttgaaatca ttgaagtagc agaacagatc gatacattta atctttctat ccagcctttt
     2341 gaagattgct gtacgatttt tgctcctcca cagccaaaaa cacgccctca actggaaaag
     2401 gtccatcaat atgaagagcg tttagcaata gatgaaatga tccaacgagc actagctggc
     2461 ttgaagatgg aagagataca agcagaatcg tctaatgaaa aaactgaaga attttcagaa
     2521 ttgctataag aggttatgaa aatagtgttt tgaccagagt attagagaaa caaaacgaaa
     2581 aaatagctcc atgtatttta ccgtttttga actaatgtcg aagattagct gattgaatac
     2641 catttatcaa gaagttatgg aaaagccggt tcttttttac taactgaaaa ataaaatttt
     2701 atttagagat caagctacag ttgataaatg atataaaaaa gagaatttcc catgaaatag
     2761 cgaggttgtg acagaagtgg gcagcttcaa gaaataagaa ggaatttacg aaaattgctt
     2821 ttcaaatttt tgtgaaattt cggcttattt ttcgaagaag ttacttctgg aacaccgtgt
     2881 atcagaggtt gcgaaaaagc tgccctgcat caagtaataa gaacagccaa acaaaaatag
     2941 ttcctcatat tttacgtttg gttgagctta ttaccgcaga tgcaagcttt tgaacaccgt
     3001 tttatttgtc tcacaccctt ttccttccta cttgtaacct tgctcccgtg ttagaaacat
     3061 gttacactaa atatgtgaaa ttattaacaa ggagcgtcgt tatgcgagaa aaaacagata
     3121 cactgccaaa agcaactgcg aaaagattac ctttatattt acgctacttg aagatgctgg
     3181 atgactcagg tataagtaga atcaaatcaa atgagttcag cgaaatcacc caaattcctt
     3241 ctgcaacgat ccgcagagac ttctcgcaat taggcgagct aggcagaagc ggatatggct
     3301 atgatgtccc ttttcttatc gatgtattca ataatatcct taatactaaa gaagaaaaac
     3361 gaatcgcatt agtaggatat ggaaatcttg gcaaagcatt gaaacacaac aattttagaa
     3421 gaaatgaaaa tctaaatatc gtgtgcgtct ttgataatga tccagcactg atcaatcgag
     3481 taattgatgg agaaatgatc tatccgattg atcgtttcgc ggagatagcc aaagcaaaaa
     3541 atgtgacagt agcaatctct acggtaccaa gtaaatattc acaatctgct attgatgaaa
     3601 ttgtcaaagg aaatgtaacc gctatactta attttgcgcc tgacagagta actgtcccag
     3661 cttatgtcaa tgttcaatac attgatttga caaccgagtt gcagacgctt atttactttg
     3721 atgagaatta ctcagaagtt ttttcttagg aaaatttgag taggcttatt gatcttgcga
     3781 gaactcgtcg taattctttc aaaaattttt tagtactaaa tgaagtggtt ttttcacgac
     3841 cacttgttga ctaaaaaaaa aaaccattgt agaatgaagc ataacgctga acaagaggag
     3901 taactggcac attcattctt agagagagaa ctatctgctg aaaggttctc aatgacaacc
     3961 agtgaaggta gcttgggagt ttctgtcttg aatggagtag agatagacga gtcgtgatcg
     4021 ttaactcact tgagaggcaa taatgattga ttattgcaat ttaggtggta ccgcgtaaat
     4081 catacgtcct agaatacgaa agtattttag gacgtttttt agtttaaagg aggaaaacag
     4141 catgacggaa gaaaataacc tatcaacaaa atataatccg caagaagttg aagctggacg
     4201 ttatcagcat tggttagaac aagatttgtt taaaccgagc ggagataaaa aagcaaaacc
     4261 ttattcaatc gtaatcccgc ccccaaatgt tactggaaaa cttcatttag ggcatgcttg
     4321 ggatacgacc ttgcaagata tgctgattcg tcaaaagaga atgcaaggat atgatacttt
     4381 atggctgcct ggtatggacc atgcaggaat cgcgacacaa gcaaaagtag aagcgaagct
     4441 tagagagcaa gggatttctc gttatgatct aggtcgtgaa aagttcatcg atcaagtgtg
     4501 ggaatggaaa gaagagtatg cgagccacat ccgcgaacag tgggctaaaa tgggcttatc
     4561 tttagattac agtagggaac gttttacatt agatgatgga ttatctgaag cagtacgtaa
     4621 agtttttgtt accttatacg aaaaaggttt gatttatcgt ggtgaatata tcatcaactg
     4681 ggatcctcaa gcacgaacag ctctatctga tatcgaagtt attcataaag atatcgaagg
     4741 cgccttttat catatgagct atgaactagc agatggatca ggcgtagtag aaattgcgac
     4801 aacccgtcca gaaacgatgt tgggagatac ggcgattgct gttcatccag aagacgagcg
     4861 ttatcaagcg ctcatcggca aaaaagtgat tctaccttta gtcaataaac aaatcccaat
     4921 cattgcagat gaatatgtag atatggaatt cgggacaggt gtagtaaaaa tcactccagc
     4981 acatgatcca aacgactttg aggtaggtaa tcgtcatgat cttccacgcg taaatgttat
     5041 ggatgatgat ggtacgatga atgaactagc aggaaaatat gctggaatgg aacgttttgc
     5101 tgctcgtaaa gcaatcgtgc aagatctgaa agaaatcggt cgattgatta aaatcgatac
     5161 gatgatccac agtgtgggtc attccgagcg gacaggtgtc gttgttgaac ctcggctgtc
     5221 tactcaatgg tttgtaaaaa tggcgccgtt agcgaaaaaa gccattgata accaagaaac
     5281 agatcaagca gtggaattct tccctccaag attcaatcaa acgtttttac gttggatgga
     5341 aaatgtccat gattgggtaa tctcacgaca attatggtgg ggacatcaaa ttcctgcatg
     5401 gtatcacaaa gaaacaggtg aaatgtatgt agggatggaa gcaccagcgg atagtgaaaa
     5461 ctgggtacaa gatcctgatg ttttagacac ttggtttagt tcagctttgt ggcctttctc
     5521 aacaatgggc tggccagata cggaaagcga agattataag cgatacttcc ctactagcac
     5581 tttagtaacc ggttatgaca tcattttctt ctgggtaagc agaatgattt tccaaagctt
     5641 ggaatttact ggcgaacgtc catttgagaa tgtcttgatt catggattga ttcgtgacga
     5701 acaagggcgg aagatgagta aatcattagg taatggaatc gatccgatgg aagtaatcga
     5761 aaagtatgga gcagatgctc tccgctggtt cttatcgaat ggttctgcac caggacaaga
     5821 tgtccgcttc agctatgaaa aaatggatgc ttcttggaac tttatcaaca aaatctggaa
     5881 tgcttcacgt ttcgttatca tgaatatcga aggaatgacc gtagaagaga tcgatttatc
     5941 tggagaaaaa tcagtggctg accggtggat ccttactcgt ttgaatgaaa cgatcgaacg
     6001 agtaactgag ctatttgatc gttttgaatt tggtgaagca ggtcgacaac tgtataactt
     6061 tatctgggat gatttttgtg actggtacat cgaaatgagt aaagaagtac tttatggaga
     6121 tgatgctgtt tcaaaacaaa ccacccgtag catcttagtc catacacttg atcagatcct
     6181 acgtttattg catccaatca tgccattcgt tacagaagaa atatgggaac atattcctca
     6241 tcagggaaat tctctagtag tggcagaata tcctgttgta cgtccagagt ttgatgatga
     6301 aacagcttct aaagggatgg aagtattgaa agaattgatt cgagccgttc gaaatattcg
     6361 ttcagaagtg aatacaccat tgtctaaacc aattacgttg ctaattaaaa caaacgatgc
     6421 taaaatcgat caatttttag ttgaaaatac aagctatatc gaacgcttct gtaatcccga
     6481 agagttgacg atatcaagtg agatcactgc gccagatcta gcgatgtcag ctgttctgac
     6541 aggtgcagaa atcttcttgc cgttagcagg attgatcaat attgaagaag aaatcaaacg
     6601 attagaaaaa gaactggaaa agtggacaag cgaagtaaaa cgtgtccaag gaaaactttc
     6661 taatgaacgt tttgtttcta acgcgccgga agaagtagta gaggcagagc gtgcaaaaga
     6721 aaaagattat ttagagaagc aaacggctgt caaagaacga atcgaaagtt tgcgtactat
     6781 tcaataagaa tagaaaaaga gagtgtgaaa tcagctagct attactaaaa gctagctgat
     6841 tgaacatcgt tacaggaaag agtttgagcc tcactcttag cttcgattca taaggagatg
     6901 tgacataaat tttgtcgcat ctctttttta gtctactctt attatgtagg agaaggaggg
     6961 aggtggatca tgtatcaaga gtcaataaag tagttgataa aatcgttcct ctctctgttt
     7021 cttttctctt tgtcatactt ttatttttct tatgtgttct atttgttata ataaaagaga
     7081 aatgggggaa ttgttgatga cgattgaaga agcaatagaa tggatccata gtcgattacc
     7141 atttggttca cgtcctggcc ttgaccgagt cgaagcttta ttagaagtgg tagggcatcc
     7201 agagagaaaa gtaaaaacga tccatatcgc tggaacaaac ggaaaaggtt ctactgtagc
     7261 ttatttgaga agcctattag aagaggctgg gctgacagta ggtacattta cttcacctta
     7321 tatcgaaacg ttcaatgaac ggatcgcaat caatggcaaa agcattccag acaatgattt
     7381 gatccagctt gtaaaaaaat atcagccact gatcaaacaa cttgatcaat ttgaagcagt
     7441 agctggtatt actgaattcg agacattaac agcaatggca tttgattatt ttttgatgaa
     7501 gaaagtggac gtagcgatta tcgaagttgg cttaggcggc ttattggatt ctaccaatgt
     7561 agctgtacca atgctgacgg gaataacaac gattggactt gatcataccg aaatacttgg
     7621 agatacaatt gaagaaatcg ctgcacaaaa agctggaatc atcaaacaag gcgttccagt
     7681 agtaacagga aatattgccc cagaagcttt tactgtcatc caacaaacag ctaaagcaaa
     7741 aaatgcaccg ctttatgtat acggggaaaa ttataaaata aactatctgc atcctgatga
     7801 gatgtgggga gaagtatttg aattttttaa tgaaatagga aagatccctc aattaaccac
     7861 tccgctttta gggcaacatc aagctgaaaa tgcaggagta gccatcgaat tgtattttgt
     7921 ttactgtcaa ttggaaaagc tgcctttccg agaaaaagat gttttgaagg gattgaagaa
     7981 atctcgatgg ccaggaagaa tggagcgttt gagtaacgag ccattagttg ttttagatgg
     8041 tgcccataat acgcatgcaa tgaaccggct tgttgagaat ttgaaaaaag aattcaaagg
     8101 atatcaaatc aacattttgt tttccgcctt acgaactaaa aatgttagag aaatgctaga
     8161 acgattgcag caagtaccaa atgtccatat ttatttgacg acctttgaac atccaaaagc
     8221 catcgaatta agtgaagtat cagatttagc aagtgagaga gtatcaatcg tttcattgtg
     8281 gcaattcgga ttagcagata tattggaaaa gatgacaaat gaagacgtat tagttgtgac
     8341 cggttcactt tatttcattt cagaagttcg ccatttatta atacaattag gaggagatca
     8401 tgaagtataa tgcagtgatt tttgacatgg atggtttgct gttcgacaca gagttagtgt
     8461 attacgaagc cacacaagta gtagcagaca agatgggttt tccttatgat aaagacctgt
     8521 atttaaaatt cttaggtgtt tcagatgaag aagtatgggc gaattatcat atgattttct
     8581 cggaatttgg caaaacaagt gtacaaagat tcatagatga ttcataccag gaaacgcttg
     8641 aacgtttttc gcaaggagcg gtacaaatga agcccggtgt gttggaactc ctctcttttt
     8701 tagaagaaca tcagattccc aaagttgtcg cttccagtaa ccaacgagca gtcattgaac
     8761 ttttattgaa gaagaacaat ctgtttgaac gatttgagac gattgtttct gcagaaaatg
     8821 ttcaacgagc aaaaccagac ccagaaattt tcttgttagc taatcagaag ctaggtacag
     8881 cgaagaaaga aacattgatt ttagaagatt cacaaaatgg tattttagca gcttctgcgg
     8941 ctgagatccc ggtagtcatg gtaccagatc tgcttccacc atcccaagaa ttgatcaaaa
     9001 aaacagctgg ggttgtttct tctttacatg aaatccctgc attgttaaaa taaaaatcca
     9061 tctcttttcg tatcttattt tagataagat agaaaggaga tgtttttttg ggaaaacgat
     9121 tgatcaaaga agtacctact agctctttac caagggaacg tatggaaatt tatggcgcgg
     9181 aagctttatc ggatcaggag cttttggcaa tcttactgcg taccggtcaa cacccttata
     9241 gtgtgatgtc gattgctgga aatctgttaa aaacgtttgg aggactggcc tctttacgac
     9301 aggctacttt gcatgagtta gaagagattc gagggatagg acgcgtgaaa gcaatcgaaa
     9361 tcaaagcctt gatcgaatta ggtcgacgaa ttcagacgga tcatgaaaga gcggctccac
     9421 cggttcgtac cagctacgaa ttggcccagc aattgattct ggaaatgaaa gatcacaaac
     9481 aagaacattt ggtttgtatt tatttggaca cgaagaatca agtgatctta aaaaaaacag
     9541 tgttcatcgg ttcgctgaat cagtcagtcg ctcatcctag agaaattttc cattatgctg
     9601 tacgttattg tgcagcaagg atcgttttag cgcataatca tccttctggt aatgttttaa
     9661 ccatagtata ataaataata gtagttaaag tagtttaaga ggagatatta aagggatatt
     9721 ttactttaaa taaaaattaa caaggtttat aatttgtagt agttaaagta tttaaagagg
     9781 tgggaacatg tgaaggttca aaggatagaa gtggagaata agccgtatcc attgtattta
     9841 ttactagata aagaatacca gctaatagaa ccagtaatga aatttattaa atacttagat
     9901 aatactggta agtctcctaa taccattaaa gcatactgct atcatttaaa gttgctgtac
     9961 gagttcatgg aacagagagg tgttattctt aatgatatta actttgagtt gttagcagac
    10021 ttcgtaggtt ggttgagata tccttcagca tcaaatgtaa ttgatcttca gtcaaaaaaa
    10081 gccataagag aagaaacgac agtgaataca attttaaatg tagttatgag ttttcttgat
    10141 tatttaagta gattaggaga atttaaatca attgatgtat ttaaacaagc aaagggaaga
    10201 aatttcaaag gatttttaca tcatgttaat aagggtagat accaaaagaa tgtcttaaag
    10261 ttaagggtta aaaagaaaca gataagaaca ttgagatcag aggaagttaa gcaaattatt
    10321 gatgcttgtc atacgaaaag agataaatta attttaatgc ttatgtatga gggtggttta
    10381 agaatcggtg aagtgttatc gcttaggctt gaagatattg tcacttggga caatcaaatc
    10441 catttaacac ctagagatgt taatgttaat gaagcttata ttaaattaag gaaggaaaga
    10501 acaatacatg tgagtaaaga acttatgtca ctttatacag attacttgat atatgagtat
    10561 agtgaggaat tggagcatga ttatgttttt atttccttaa aagaaggcta ttttgggaaa
    10621 ccactaaagt accaaagtgt tcttgatcta gttagaagaa tagttaaaag gactggaata
    10681 gaatttacat cacatatgct tcgccacact cacgcaacgc agctaattag ggaaggatgg
    10741 gatgttgcgt tcgttcaaaa gagattaggt cacgcacatg ttcagacaac gttaaatacc
    10801 tatgttcatc tttcagatca ggatatgaaa aatgagttta ataaatacct cgagagaaag
    10861 gagcataaga aatgaatgct tctagtaaaa ggaaaattat tagtcagagt gagattagca
    10921 aaaaaatagc tgtaatgaat gaagaaatgc aggggttttg ggctaataat agttgggata
    10981 taagaaaatg tccacatcct tctgccatag aattaagtaa gaatcctgct ttaaggaatc
    11041 gttgggttcg ttttgaacgt gttaaaaatc tgtggttaag aacagaattg aaatattttt
    11101 atttttacca tttaaacaat ggaatatgga atgcaaaaac tgtctggatt agaaaaggaa
    11161 cagtaattaa taaaatgtta gatttcttag atttaaagta tcctagcatt acttcaatta
    11221 ctgaagttcc tattgaaaaa gcaatgacgg agtatagaac ttatttgaca aaacggggtg
    11281 ttagaattac cactactaat tataagatta ctgctaatca agaaaaaaca cctgtaaaag
    11341 ctaattccta ctatgttact aatctaaaac aatttatgga gttttatgag aacttttatt
    11401 ttgatggaga ggagtgggat aaagacgttt gggatagacg taacttacct ttgccagatg
    11461 ataaggttaa cccaacacaa tatgaatata caattaactt taaagggttt cggaatacat
    11521 attttaaaca acttgtaaaa agatattgta agttgagatt gaacatggat agcttttcct
    11581 atgtaagtga tattgcccaa agacttaaag agttctttaa ttttctggac atgaaattta
    11641 aacaagttca gagagtacac caattaacga gagtggaaat tgaagcatat ttaagtgaac
    11701 taaacatgat gggaataaaa cctagtacaa taactgggag gatctctata ttggaaggac
    11761 tatttagtac ccttcttagg ctagaatggg atgatgttcc ttccaaaata ttaatttatt
    11821 ctgaggacta tccgaaaata ccaagagcaa aaccacgctt tatagatgaa ttcgtcctag
    11881 agcaattgaa cagtcatctt gataaattac ccgaatatat agctacgatg actatgattg
    11941 ttcaagaatg tggaatgagg ataagtgaat tgtgcacctt gaaaaaaggc tgtctattag
    12001 aggacaaaga tggagatttc tttttaaagt attatcaatg gaaaatgaaa aaggagcata
    12061 tagttccaat atctaaagag gtagctttac ttattaaagt tcgggaagat aaagtttcag
    12121 aggaatttcc agatagtgaa tacctctttc caagaaaaga tggatcgcca ttaaaacaag
    12181 aaacatttag aggtgagtta aataaattag cttatgagca aaatatagtg gataaatcag
    12241 gtgagattta tagattccat gcccatgcct ttcgccatac agtaggaaca agaatgatta
    12301 acaacgggat gccccagcat attgtgcaga aatttttggg gcatgaaagc ccagaaatga
    12361 caagcagata cgctcatatc tttgatgaaa ctctaaaaaa tgaatttact aaatttcagg
    12421 aaaaactggt taccaataat ggagatgtgc ttgatctaga tgaagataat gaagtcgatg
    12481 atgtagagct tcaatggttc aagaaaaata taaatgcaca agtgcttcca aatggttatt
    12541 gtagattgcc agtagtagca ggtggttgtc cacatgcgaa tgcatgctta gattgcactc
    12601 acttctgtac cagtaagcaa ttcttaccac agcacgaaga acagttagag cgtacagaag
    12661 agttattagc catagctaag gataaacaat ggcaaagaca agtagagact aatagccgtg
    12721 ttaaagagcg tttagaacaa atcattggaa gtttgacggg gtaattatca atggataaac
    12781 aagttagaaa tacaacagaa attgtacgtt tggcgaagca gaaatcaaaa aagacaaggg
    12841 aaaaagtaga caaagcgatt tctaaatttt cgattgaagg taaagttatt aattttaatt
    12901 caatagcaaa ggaagctaat gtttctaaat catggcttta taaggaacac gatattaggc
    12961 aaagaatcga atcccttcgt gagcgtcaaa taacagcaaa tgtagtctca aaacccaaga
    13021 aaagttctcg ttcggaggaa atccttatta aaaccttaaa aagaagagta atggaattag
    13081 aaaaagaaaa taaaaaatta cagaaccaaa ttcaaaaatt atatggagat ctgtataata
    13141 aagaataatt attaatctgt agacaaattg tgaaaggatg tacttaaacg ctaacggtca
    13201 gctttattga acagtcattt aggtatatgt ccaatctagg gtaagtaaat tgagtatcaa
    13261 tataaacttt atatgaacat aatcagttac gaggtgaaat catgagcaat ttgattaacg
    13321 gaaaaatacc aaatcaagcg attcaaacat taaaaatcgt aaaagattta tttggaagtt
    13381 caatagttgg agtatatcta tttggttcag cagtaaatgg tggtttacgc attaacagcg
    13441 atgtagatgt tctagtcgtc gtgaatcata gtttacctca attaactcga aaaaaactaa
    13501 cagaaagact aatgactata tcaggaaaga ttggaaatac ggattctgtt agaccacttg
    13561 aagttacggt tataaatagg agtgaagttg tcccttggca atatcctcca aaaagagaat
    13621 ttatatacgg tgagtggctc aggggtgaat ttgagaatgg acaaattcag gaaccaagct
    13681 atgatcctga tttggctatt gttttagcac aagcaagaaa gaatagtatt tctctatttg
    13741 gtcctgattc ttcaagtata cttgtctccg tacctttgac agatattcga agagcaatta
    13801 aggattcttt gccagaacta attgagggga taaaaggtga tgagcgtaat gtaattttaa
    13861 ccctagctcg aatgtggcaa acagtgacta ctggtgaaat tacctcgaaa gatgtcgctg
    13921 cagaatgggc tatacctctt ttacctaaag agcatgtaac tttactggat atagctagaa
    13981 aaggctatcg gggagagtgt gatgataagt gggaaggact atattcaaag gtgaaagcac
    14041 tcgttaagta tatgaaaaat tctatagaaa cttctctcaa ttaggctaat tttattgcaa
    14101 taacaggtgc ttacttttaa aactactgat ttattgataa atattgaaca atttttggga
    14161 agaataaagc gtcctcttgt gaaattagag aacgctttat tactttaatt tagtgaaaca
    14221 atttgtaact attgaaaata gaaagaaatt gttccttcga tagtttatta atattagtga
    14281 catttgcatg cttcaaagcc tgtcggaatt ggtttttagt gaaaagaaca cgatattcac
    14341 ggtttaccca cttataaaca aaagatcgat actttttgta gtccttcttt gaaatcaatg
    14401 gttgatgtcg ttcaagaaca atcaatacag agtctacact tggcttagga tgaaaatata
    14461 gtggtggtac ttttttgagc atttttatat ccatctccac cattaatagt aaacccaaag
    14521 ctcgttgcag attttgcaat cttttcgcaa atcccttctc aacgataaga tagctatatt
    14581 tagcctgact ttcaaaggta attcttttga caatatccgt actgatgtta taaggaatat
    14641 taccatatat cttatagttt atatgttttg ggaaggaaaa ttttagaata tccgtttgaa
    14701 tcacttttat attctcagag gggtttaccg cttctttagt cacttgacat aagcctccat
    14761 caatttctat agcagtaact gatcgactca ttttgactag ctctttggta aaatgtcctt
    14821 ttcctgatcc gatttctatt acgttgtctt gtttactgat attcgtgtga ttcaatattt
    14881 cttttacatg ctttttagaa gtaataaaat tttgcgtgtc tttagggttt ttctggttca
    14941 ttataacctt ctccttactg gttataatga actgacatta agcgttcatt ataaccaatt
    15001 aattttgatt tggttgataa cgaactttat taataacaaa tatagaaaaa gtacccatac
    15061 ttttatctcc tttccatttt tttcattatg aatgagataa agtaatttct actactgcga
    15121 tactggtgca cataattgaa acctccttat gtaactgaat atgattgtat catcttttca
    15181 ttttagaata aacctttata tgtaattttg cacttgacac ttcagatgga cgttttcttc
    15241 aaaataatat gttttgaaac ttgataagag aagccactgt ctttactaaa aagtaaaggc
    15301 tgcgataaag gacagtgact ttaataaagt tgcactgttg tattgtcttt ggataaaatt
    15361 gtctaaaggg aagttttatc cccaacgaaa aaacatctaa agtggtaagt aatgaaagaa
    15421 tgtcgataaa gtaggaatgg gggatctttt tgaatacaat agaatcacag tttgataagg
    15481 ttgcagaaga ttacgatttc gtgaatgagc ttttgaatga ttattcattc tttgtgtcta
    15541 atatgtctcc aaagaaaggc agagcattag atatcggatg tggctcgggt ttgttagtgg
    15601 agaaattagc aagttattat gatgaagtgg tagggattga tatttctaat caaatgctcg
    15661 atcttgccaa atctaaacgt caactaacaa atacggtcta tctgaatatg aatgcagaac
    15721 aacttaattt taatgagaag tttgatttta ttgtaagccg aacaaccttt catcatttgg
    15781 atgatatagc cagcgtaata cagcaaatga aggaactgtt gaatgaggaa ggaagaatag
    15841 ttattcttga taatgtatct gaagtcgaga caccgcctac ctatgtttat aaattagggg
    15901 caatccaaga gtttttaccg cattgtttca aatttggaat aaaaaatgcg attagaattt
    15961 ataatcacaa cacatctaaa tcatggcttg agcatttagc ttcagataag tacctatctg
    16021 aacaaaatta ttacgattta tatgaaaagt tgttgcctgg atgccaattc cataaaatgg
    16081 gttgggcaat gggggttgtc tggacaaaat aacttaaagc tgtggctttg aaaagttgaa
    16141 tttgtgattg ttttcagagc tatcgggtgc tttagtataa ataacaatga ataaaaaatc
    16201 agcctgtatc ttatgttact attaagtaat ttaagataca ggttttacta tttttgaaat
    16261 aagtaccttc cacttcaaat acatgtattc aaagtgaaag tattgattta aatgattctt
    16321 ttgtaattta ctcttgataa tatgaaattc catcgcagca ggatatgaat tttaccaaaa
    16381 ggatacaaaa gtgtggagaa atgatgggga tcactgtact ggaccacttg atcatcggac
    16441 gcaaacgata ttttagtttg agagaagaag gaatgatgga agagaagtaa gttgctttct
    16501 tgcaaaaaaa atgaagcaag tgtacaatta tattgtaatt ttgtttggga atagaagttg
    16561 aaacatctgt tgcacctcta tcgtacacga gcaatgttac aatttaaatt agtgcttttg
    16621 gcacgaggag gatattatta tggaaaacgg aacagtaaaa tggttcaacg ctgaaaaagg
    16681 atttggattt atctcacgtg aagatggaag cgacgtattc gttcacttct cagctatcca
    16741 aggtgacgga ttcaaaacat tagaagaagg tcaagcagtg actttcgacg ttgaagaatc
    16801 agaccgcggc ccacaagctg caaacgttgt aaaagcttaa ttttatctgt aaatcaaatt
    16861 gatggtagac atcaattttt acgagagaac aaagcgagta acaaaaatac ctgccacaag
    16921 agacaatttc tcttgtggca ggtatttttt tgtatgaaaa agtaaataaa tttaagaggc
    16981 tatgaaagag ccaccttgta tcaaggaata agaacaaaca aaaatggctt ctcatgtttt
    17041 tcatttgttt tctcttatat tcaggacaag gtgctgattt cataacctct tatttattct
    17101 tcatctggtg tcattaccat tggcaaaatc attggatgtc tttctgtacg ttcgaataag
    17161 aaaggctgaa gcgcgccagt catcgcttct ttcaatttat attctgaaca gtcaggtcca
    17221 ttcaaacatt ctcttaaagc gtggaataag attcgctgag cttcattgat catttctcct
    17281 gattctcgca tgtagacaaa tccacgagag agaatatctg gaccagccaa gatttctttt
    17341 ttcttgatat ccactgtcgc gacagctaat acaagacctt cttctgaaag caggtgacgg
    17401 tcacgtaaaa cgacattacc aatatcaccg atcccgctac catcgatata cacatctcca
    17461 gccacaaaat gcccagcagg acgcgcagaa tcgctagtta acgctaaaac atcgccattt
    17521 tccatgatga agcagttttc tgcaggaaca cctacatctt gtgctagaga agcatgtact
    17581 ttcaacatgc ggaattctcc gtggacagga ataaaatatt ttggcttcat caaacgaagc
    17641 attaattttt gttcttcttg tccaccgtga ccggaagtat ggatgttatt gatttttccg
    17701 tgaatcactt ctgcaccagc ttcagacagc aaattgatca aatgattgac acttgttgta
    17761 ttaccaggaa taggtgaact tgaaaagatc actgtatcgt ctggctggat ttggatttga
    17821 cggtgagtac cattcgcgat gcggctcaac gcggccatcg cttctccttg agaacctgta
    17881 cataggatca tcgtttcatt ggcaggcaag cgattgattt cgtgtgcatc tacaaatgtg
    17941 ccgtctggaa ctttgatata tcctaaacgt tgaccgttga cgatggcatt ttccatgctt
    18001 cgtccgaaaa cagcgatttt acgaccagtt gccacagcgg catctgctgc ttgctgcaaa
    18061 cggaagatgt tagaagcaaa gctggcaaaa atgatacgtc cttcgatttt tgaaaagatc
    18121 ttcaaaatag aatgcccaat cgttttttct gatttagtaa atgttggaat ctcagcattt
    18181 gtactatcag ataaaaggca caagactcct tcttccccga tacgagccat tttatgcaag
    18241 tttgctggtt cgccaacagg agtgaaatcg aatttgaagt ctccagtcgc tacgatattt
    18301 ccagaaggtg ttttcaccac gatccctaat gcatctggga tactatgggt cgttctgaaa
    18361 aagctgacag aagtctttct aaaacggatg atcgtatctt cattgatctc atggagctct
    18421 gcatctcgaa gcaagccatg ctcatctaat ttgtttgtga tcaacgctaa tgcgagagga
    18481 ccagcataga taggaatatt tgcttgtttc agcaagtagg gtattccgcc gatgtggtct
    18541 tcgtgcccgt gagtaatcac caaggctttt actttgtgta agttttgtat gatgtagctg
    18601 tagtcgggaa tgacatagtc aattccaagt aaatcgtctt ccgggaattt gatcccggca
    18661 tcaatcaaaa tgatttcatc ttggaattgt accccatacg tatttttccc gatttcacct
    18721 aatccaccaa tagcaaagac gcctgtctcg ttatttttga tggaaagttt catattaaaa
    18781 ctccactaat gagaaatcag cattttcttt ttcgtactct agatgacttc cttctagaag
    18841 ttgaacgaat tcaatattgt atggggtatt ttcttccaca cgttggcgag cgtctacatc
    18901 gctttttgcc tcaatgtaaa gagattttgt ttcttcccgt ttagggttgc gtacttttgt
    18961 ttcttgataa taaactttat agatcattgt tttgtctcct ttatctgcgt aaagcagtga
    19021 tttagctagt tgattttttt agacaacaca aaacgagggg acaagccacc tggtttttca
    19081 tatgctttga ctagaatact ggattcgagt caaagagaca agcctcgttt ttgcttatct
    19141 gttcggatat tttttcaacc gtaccttctg tataaaaaat atacaacaat taattgttat
    19201 tctagcataa aacagcaaat aagtatagaa atcaactaga ttgaaagaaa aaatcaatgt
    19261 ttatcatccc gctataatca taatgacttt tttatgagag aagtgttcta ttaataggct
    19321 cgttgtgcta tcatttttag taatgaaaat gtaacaaaag attaaaagga gatgagaaag
    19381 tgaaactaat gtggcattat accatgcgtt ataagaaatt cttgtttttc aatttcatat
    19441 gcgtatttgg ttttattttg attgaattag gacttcctac gattttagca cgtatgatcg
    19501 atgtaggaat ccgcaatgat gattttgatt atgtgaagca acaagggata tttatgatcg
    19561 ttattactgt aatcggtatt attttgaata tcttgttagg ttattttggt tcgaggatta
    19621 cgactaatat cgtagcagat attcgagatg atttgttcga aaagatccaa agttattcgc
    19681 atcaggaata cgaaaatcta ggcgtatctt cattgatcac tcgtacaaca aacgatgctt
    19741 atcagatcat gttgttcctg caaaatattt tacgtatcgg ttttatggca ccaatgatgt
    19801 tcattgtcag tttgtacatg gtcatgcgta caagtgtgac actcagtttg tatgtagtag
    19861 gtgcattacc tttactatta ttggcagtcg tagcgattgc taaggtgtct gaaccgttat
    19921 ctaaaaagca acaaaaaaac ttagataaaa tcaacagtat cttgcgggaa aatctttcag
    19981 gattgcgtgt gattcgtgca ttcgtcaatg aaaagtttga agaaaaacgt tttgctgtag
    20041 tcaatgagaa ttatgcttct agttcaaaaa gcttattccg tttgatggct gttgcacaac
    20101 cgggttttta tttcttattt aatatcgtaa tggtattgat catctggagc ggggcagtgc
    20161 aaatcgatgc aggcgagctt cgtgtaggag atttgattgc gtttatcgag tatatattcc
    20221 atgccttgtt ctcattcatg ttatttgcta gtgtgttcat gatgtatcca agagcagctg
    20281 tttcagctgg acgtatccaa gaagccctag atgccaaacc ttcgattgta gaagatccaa
    20341 atggtatatc tgatacaaaa actaaagggt atatcgaatt tcaaaatgta acatttgctt
    20401 atccaggcca ttcgcaagag ccagtgatac gaaatgtcag ttttacagcc tctccaggag
    20461 agaccgttgc ttttattgga agtaccggta gcggaaaatc tacgttgatc caattgatcc
    20521 ctcgctttta tgatgtgagt gaaggaaaga tcttgatcga tggagtagac gtgagagatt
    20581 atcggctaag caagttgcga gataagattg ggtttattcc gcaaaaagcg ctgctattta
    20641 ctggaacgat tgcagataat ttgcgatacg ggaaagaaga tgctacgcaa gaagaaatgg
    20701 aacgtgcagc tgagattgct caagcttcag aattcatttc tcgaaaacca gatggattta
    20761 atgaacattt atcagaaggt ggttccaatt tttcaggtgg acaaaaacaa cgattggcaa
    20821 tagccagagc catcatacgc cgtccggaaa tctatatttt tgatgacagc ttttctgcat
    20881 tagattatca aacggatgcg aagttacgtg ctcgtctgaa aaaagaaacg acagaatcta
    20941 ctgtattgat cgtagcacaa cgtgtgggaa cgatcatgca tgcagataag atcatcgtat
    21001 taaatgaagg agaagtcgtc ggtatgggga cgcatcggga gttgttggaa aaatgcccga
    21061 tttattatga tattgccgct tctcaattat caaaggagga attagcatga aaaaaacctt
    21121 ttcttcctta aaacgtttag ctcgatatat cagaccgtat cgtgagactt ttcttttagt
    21181 gatcctattt acagttctga cagtggcttt caacgtggct ttgccatatg ttacaggact
    21241 tcctactaca gaaatcagcc gtaatattgc caatggcgaa tcaatcaatt tttcttatat
    21301 attcaaatgt ctcatttgga tcacagttgt tggaataggg tattgtattt cgcaattact
    21361 ttctggtgtt ttgatgacaa atgtcgttca aagtgccatg catgatttga gaagagacat
    21421 agaagaaaaa atcaatcgtc ttccggtttc atatttcgat aaaaaccagc aaggaaatat
    21481 tttgtctcgt gtgacgaatg atgtagatgc agtcagcggt gctttacaac aagcttttat
    21541 tggtatcgta aatgcgatat tgggaattac gttggctgca gcaatgatgt tttatatcca
    21601 gccgatgatg gcgctgatct ctatgatcat gattccaaca tcgatctgga tctcaaaacg
    21661 agtgatcaat gcttcgcaga aatattttca aagcatgcag aattctcttg gtgagctaaa
    21721 cggctacgtc caagaaaata tgacaggttt tagtgtattg aaagtatatg gacgcgaaaa
    21781 agaaacattg gaaggattca aaaaagttaa tcacagcttg aaatactttg ggtttcgtgc
    21841 agcattcatt tctggcttga tgatgcctct agttcaatta actgcctatg ctacttatat
    21901 tggtatggct gttctaggaa gcttttatgt tattactggg gcgatcgttg tcggtcaact
    21961 acaagcattc atccaatata tttggcaaat cagtcagcca atggggaacg tgacacaatt
    22021 gtcttcagtt ttgcaaagtg catcagcggc aacgaaacgt gtattcgaga ttttagatga
    22081 gccagaagaa aaagaaaaca aagtagatgc ttcactgcca gaaacgattt taggagatgt
    22141 gacattcgat catgtcgatt ttgcatatga tccgaagaaa ccattaatcc aagatttgaa
    22201 ttttgaagtg aaagctggtc aaaccgtagc tgtggtcggt ccgaccggtg ccggcaagac
    22261 gacattgatc aatctattga tgcgttttta tgacgtagac aaaggagcaa taaggattga
    22321 tggaatcgac accaaatcaa tgagtcgcag tgatgtgcgt tcgctatttg gtatggtctt
    22381 gcaagatgca tggctttatc aaggaacgat tgcagataat atccgcttcg gaaagcttga
    22441 tgcgacagat tatgaagtag ttgatgctgc gaaaacagca aacgtagatc attttatccg
    22501 aacgatgcca gatggatatg atatggccat caattctgag ggagacaatg tgtctttagg
    22561 acaaaaacag ttgctgacga ttgcccgagc agtggtctcc gatcctaaaa tcttgatttt
    22621 agatgaagca acaagttcag tagacacccg tttagaagcg ttgattcaaa aagcaatgga
    22681 tcgcgtgatg gagggacgga caagtttcgt catcgctcac cgtttatcga caatccgtga
    22741 tgcagatctg attctagtga tggatcaagg tcagatcatt gaaaaaggaa cgcatgagag
    22801 tttattggca caaggtggat tttatgaaaa actgtataac agccaatttg ctgaggaagc
    22861 agaataagtc atataagaat aaaacagttt aaaacttatt ttttgttctc tattcttgat
    22921 aatggtaaag taaagttatc aagaaaagtg aggacacgga gatggaacag tttttaaatg
    22981 aaaaaattac tgaagccaaa agagaagttg atcgtttaac gcacgtctgt cagacgaagc
    23041 cttttgattc agctgatttt tttgaaaatg agtttcaaat cgaacgcttg gcagcacaaa
    23101 tagaagtgta cgaattaatt ttaaaaaaag taatagaaga aaatatagat gtacaataaa
    23161 aacaggtaaa acgtcagtta ccagatactg taaatagaca tctggcctga gcaggtttta
    23221 cctgtttttt ttcttatgaa ataaatatat gctatgatac ctttattcaa taggattagt
    23281 ttctacaaaa aatgaaagga gaaaaataaa tatggaacgg tttatagcat caaatgccac
    23341 agtgattggt gatgtgacat tgtcagaaga tgtgacgatc tggtatcaag ctgttttacg
    23401 cggggacagt aattggataa aaataggaca aagaacgaat atccaagatg gtacaatcat
    23461 ccatgtcgat catgatgcac ctgtagatat tgcggagaat gtgacggtag gacatcagtg
    23521 catgctacat ggctgtacaa tcgaaaaagg ggcgctgatc gggatgggga ccacgatatt
    23581 gaatcatgcg gttattggtg agaatagttt gattggtgct ggatcattag taacagaagg
    23641 aaaagttatt cctcctaatg tcttggcttt tggtcgccca gctaaagtga tccgtccttt
    23701 gacaaaagaa gaaatacaaa aaaacaaaga aaacatccag cattatgtcg agataggtcg
    23761 gatgcatgcg caaggaaaat tcaaggagca aagataggca gtagcatcca taaaataaaa
    23821 aaggatggtc agtttgagtt gaccatcctt ttttatttgg tttagataag agagcttaaa
    23881 aaagcttcct ttgaaacaga ttaatttaat gaagaactgt tttccatgac ttcatcgatc
    23941 aatccatatg cttttgcttg ttcagctgtc atatagttgt cacgatctgt atctttttca
    24001 atcacttcaa gcggttgtcc agtgcgttca gctaaaattt tgtttaagcg ttcgcgtgtt
    24061 tgcaaaatat ggcgtgccgc aatttcgatt tcagttgctt gcccttgagc gccgccaaga
    24121 ggttgatgga tcatgatctc agcatttggt aaagcaaatc gtttaccttt tgtaccagct
    24181 gtcagcaaga agcttcccat tgaagcagcc atacccataa caattgtttg gacatctgct
    24241 ttgacaaagt tcattgtgtc gtaaatagcc atacctgctg taactgaacc accaggagaa
    24301 ttgatataaa gatagatatc tttttcagaa tcttgagcgt ctaagaaaag caattgggcg
    24361 atgatcgaat tagcaagatc atctgttact tggccgctta acatgatgat acggtctttc
    24421 aataaacgtg agtagatatc gtaagctctt tcacctcgtg aagattgttc aatgactgta
    24481 ggaattaaat tcataataga gttcctcctt gatttcgttt agaaatagtg taccataatt
    24541 tagtttctaa tacctaaaat gtagtataca tttttggtca aaaaaggtca aataaaaaac
    24601 ttaggcaaat tagtcgcata aacccgcttt atggtatgat aagcagagaa acaaaacaag
    24661 gaggaacaac agattgatag ttggtattac tggccttatc ggagtgctga caggtgctgt
    24721 agttgtctat atcggcatga tactgcaaat gcgtcacttg gaaaataaag aacagcgtag
    24781 acgtgaattg gaattgaaag tcaaagaaat cgagacgctg aatcaattaa ataaaaagat
    24841 caacgagatc ttgcaaaaac ggacaacgct gatggaagaa tatgtcagct ttgatgcatt
    24901 tgatgattgt tatatcacga ttgacgattt tgcgtatctc caatcatttt cagcgcaaaa
    24961 tagtttttat ctgccaaatt atattttaga cgagctattt aagaatatct cccatcgaaa
    25021 agtgatttta tctccagatg aaacaatgaa gattggtgga tatgcttata aaggcggacg
    25081 cgtgatttta gagaatttta cggatagtct gactgaaatg atcaatgaaa aaaaagctca
    25141 gttgaagact atgacgcgcc agccgattgg tttcttctcg aaagatgatg tttggagctg
    25201 atattttgaa gcattgccgt tgcacatgag agcgtggtaa tgctttcttt ttttcttaat
    25261 gatacaatag aactgaacgg agtgaagaaa gatgttacaa atctatgccc atcgcggaag
    25321 cagcggcacc cacccagaaa atacgcttcc tgcatttgca gaagctgtac gagtaggtgc
    25381 agatggtatc gaactagatg tccatctaag caaagatggt tatttgatcg ttatgcacga
    25441 cgaagaagtg gaccggacga ctaacggtaa agggctgatt cgagaaaaaa cattggaaga
    25501 actcaaaaaa ttgaatgcag ggggcaaatt cagcaaagaa tttccagcag ctaaagttcc
    25561 tgaattgaga gaagtgttag gactgctcct ccaaaaaaat taccgaggga tgctaatgat
    25621 tgaaattaaa actgatcagt atgaatatcc cggtatcgaa gaaaaattag ccgaaacatt
    25681 aaaaagtaga gattggcctt ttcaacatgc atattgcagc tttcatttgg attctttgga
    25741 aaaaatcgca ttactggaac cggagataca gctggactta ttgatgagta cttctgataa
    25801 aaagatccaa ctagcagcag aaacccctta tatagaagga gtacatccaa aaaatacttg
    25861 gttgttcgaa catccagtcc aggcagctga ttttccaaaa gcaattcgtg catggacgat
    25921 taacgatgaa gaagaaatgg ccaaatgtca agaactcaaa atagatggaa taataactga
    25981 ttttccggag aaagctcttc ggtggaaaca atcaacgaaa agtaggtaaa atatggaaaa
    26041 agtattagtg atcgtcggtc cgacagcagt cggtaaaaca gcgctaagtg tggaactcgc
    26101 acaactattt ggaggagaga tcatcagtgg tgattcattg caaatctata aaaatctgga
    26161 tatcggtaca gcaaaaatca aagaagaaga gatgaataat gttccgcatc atttgattga
    26221 tgtgatcgaa cctactcaac gttactcagc cgctgatttt cagcattcag gtcgacaatt
    26281 gattaccgag attgcgaacc gagggcacct acctatcata gctggtggta cgggtctcta
    26341 tatccaatcc cttctatatg attatcagtt aggagcgaca gaagaagcga atcctgagat
    26401 tagaaaaaag tatgaaacgt tagctgaaac aattggttcc caagcattat ggaatcagtt
    26461 gaaagaaaaa gacccggcag ctgctgagaa aatccactgg aacaatcaac gcaaagtgat
    26521 aagagctctg gaagttttag agacgacagg acatagtatc acggcaccta aagaagagcc
    26581 aaagcaattg tatgattacc tcatgattgg attagatacg aagcgtagta ttctttatga
    26641 acgaatcaat caaagagtcg atttgatgct agcagaaggt ttagtagcag aagcgcgtaa
    26701 ggtgtatgag cttggagaag tccaggccag tcaaggtatt ggatataaag aatttttccc
    26761 ttattttgaa gggaaagaaa cattggaaga agcaacagaa caagtcaaac tacattcgag
    26821 aagatacgcg aaacgtcagt tgacgtggtt tcgtaaccgt ttggatgcac agtggtttga
    26881 tcttataagt gagccaaatc aaaaaagtca gattgagcaa acaatcaaaa aatggctgga
    26941 ggcggaataa aatggaagaa aaagtgatca tcgtaggggt agaaaccgaa gcaaaccagc
    27001 gttacttttc agagtcaatg gaagagctag ttcagctgac gaataccgca tcaggagaag
    27061 tcgtattcac aattacacaa aagagaccac aagtggatcg gcaaacgatt atcggtaaag
    27121 ggaaattaca agaattagtt caacaagcgg atgcgcatga agcggatttg atcatcttta
    27181 atcatgagct aactccgaga cagagccaac tgattaccga tgctgttggt cttccggtaa
    27241 ttgatcgcgt gcagcttatc ttagatattt ttgcgatgcg tgctcgttcg aaagaaggga
    27301 agctgcaggt tgaacttgct cagttagaat acctgctgcc tcgtttagta gggcaaggga
    27361 aaacattatc tcgtcttggc ggtgggatcg gaacaagagg gccaggtgaa acaaagttag
    27421 aaacagaccg ccgacatata cggaataaaa tttttgctgt taagaaagaa ttgaaagaag
    27481 tagaagctca tcgagagcgc aatcgccaaa aaagaaaaaa tagcgagatt tttcagattg
    27541 gacttattgg ctacacgaat gccgggaagt caactatctt gaatctattg acacaagcgg
    27601 atacttattc gaaagatcaa ttgtttgcaa cgttagatcc attgactaag agatggcgtt
    27661 ttgctgaagg atttgagatt actgtaacgg ataccgtcgg gttcatccaa gacctgccta
    27721 cgcaactgat cgatgctttc cattctacat tagaagaaag tcaaaatatg gatcttttgc
    27781 tacatgtagt cgatgctagt tctccagatc gtattttaca ggagcagact gtcttaaaat
    27841 tgatggatga actgaatatg aaagaaatgc cgatactgac tgtttacaat aaggcagacc
    27901 agatcgaccc agcgatgttc acgcctacgc tattcccaaa tgtattgatt tctactcaga
    27961 cgaaagaagg taagatggcg ttgatcgaag caatcaaacg tcagctgatg gaactaatga
    28021 ccccttatac actttctgtg ccaagcaacg aaggacagaa attaagcgag ttgagaagac
    28081 aaacaatggt tttggacgaa gcgtacatgg aagaaacaaa cgaatatgaa gttaaagggt
    28141 ttgcaaaaac caattcaaaa tggttaagaa aaaaccaata aaattttatt tttttaa
//
LOCUS       DAJSBL010000032        27270 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.032, whole genome shotgun sequence.
ACCESSION   DAJSBL010000032 DAJSBL010000000
VERSION     DAJSBL010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27270
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.032"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            150..1577
                     /locus_tag="ONT85_001684"
     CDS             150..1577
                     /locus_tag="ONT85_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR2378659.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(1669..2034)
                     /locus_tag="ONT85_001685"
     CDS             complement(1669..2034)
                     /locus_tag="ONT85_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HCR2378660.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPLDVQNK"
     gene            complement(2031..2396)
                     /locus_tag="ONT85_001686"
     CDS             complement(2031..2396)
                     /locus_tag="ONT85_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HCR2378661.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(2588..2836)
                     /locus_tag="ONT85_001687"
     CDS             complement(2588..2836)
                     /locus_tag="ONT85_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2378662.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            2835..2963
                     /locus_tag="ONT85_001688"
     CDS             2835..2963
                     /locus_tag="ONT85_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HCR2378663.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            3020..4714
                     /locus_tag="ONT85_001689"
     CDS             3020..4714
                     /locus_tag="ONT85_001689"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HCR2378664.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            4718..4837
                     /locus_tag="ONT85_001690"
                     /pseudo
     CDS             4718..4837
                     /locus_tag="ONT85_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349726.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
     gene            4814..4987
                     /locus_tag="ONT85_001691"
     CDS             4814..4987
                     /locus_tag="ONT85_001691"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2378665.1"
                     /translation="MQAFEHRLPRGYEKAALHQVIRAAKLKQLLMFLTWLDLLPQMQA
                     FEHRLPRGYEKGV"
     gene            5365..6498
                     /locus_tag="ONT85_001692"
     CDS             5365..6498
                     /locus_tag="ONT85_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HCR2378666.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            6529..6849
                     /locus_tag="ONT85_001693"
     CDS             6529..6849
                     /locus_tag="ONT85_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhK family protein"
                     /protein_id="HCR2378667.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(6878..7342)
                     /locus_tag="ONT85_001694"
     CDS             complement(6878..7342)
                     /locus_tag="ONT85_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR2378668.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(7474..8727)
                     /locus_tag="ONT85_001695"
     CDS             complement(7474..8727)
                     /locus_tag="ONT85_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCR2378669.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVLVSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAAVNLV
                     LSILIYLPFVKVATIQERRKEALDA"
     gene            complement(8756..9073)
                     /locus_tag="ONT85_001696"
     CDS             complement(8756..9073)
                     /locus_tag="ONT85_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HCR2378670.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIINLYRR
                     LNA"
     gene            complement(9099..9419)
                     /locus_tag="ONT85_001697"
     CDS             complement(9099..9419)
                     /locus_tag="ONT85_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCR2378671.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(9883..11298)
                     /locus_tag="ONT85_001698"
     CDS             complement(9883..11298)
                     /locus_tag="ONT85_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HCR2378672.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(11315..12631)
                     /locus_tag="ONT85_001699"
     CDS             complement(11315..12631)
                     /locus_tag="ONT85_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCR2378673.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(12781..13479)
                     /locus_tag="ONT85_001700"
     CDS             complement(12781..13479)
                     /locus_tag="ONT85_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HCR2378674.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            complement(13768..14724)
                     /locus_tag="ONT85_001701"
     CDS             complement(13768..14724)
                     /locus_tag="ONT85_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HCR2378675.1"
                     /translation="MEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQREILRGAFDMG
                     ITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMWEGPYGEWGSRK
                     SIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGKALYIGISNYNG
                     EDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGAITFSPLAQGLL
                     TNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQSLAQMALAWNLR
                     QKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
     gene            complement(14837..15670)
                     /gene="msrA"
                     /locus_tag="ONT85_001702"
     CDS             complement(14837..15670)
                     /gene="msrA"
                     /locus_tag="ONT85_001702"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HCR2378676.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQVERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            complement(15845..17479)
                     /locus_tag="ONT85_001703"
     CDS             complement(15845..17479)
                     /locus_tag="ONT85_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2378677.1"
                     /translation="MKINKKVVILSGLIIVSSIYSFIFFKNTQSIYTTGDLSFHLSRI
                     KGLSTIFTSPINYETFNYTGYGVNYFYPFLTFFPAVMLYWMTKNLIVSYIIYVWLLNL
                     CTTLVAYHYGERFLKQKKAAFLFSCLYIFSAYRTVDIYYRSAIAEAIAITLVIPVLFY
                     AYQIISGKEEKYPSVKLALSMSLLVYSHVLSTLMSTALIIIFIFIRLVSKGFKNADFI
                     AIFKKLFSAAGMTLVLTSAFWYPMFEQMLYQKINKPSVTNLYAHASNVFDSLTEAMNN
                     DLTTYSMGLVGLLSLCIPLILFKKLTNIEKKIYYGTCLTWLATTSLVPWYLLQNTPAK
                     LLQFPWRILSLQIIFSSLILTMIFFKNRRYNKTRELFYLFMTVLLLITLTVSSKKNFN
                     QKIISQPGHIVVNKETVEAYTTKGMDYFLYDYSPIASLENKEHIIKHEAKIGSQWVNL
                     PYKTTGSSIIYRVTNEKSQVITLPVYAYLGTNVEINHQPVKEQVNANGLVQISGVKGV
                     ADIKVSTSYTVTTIISYLVTLTAYLCLFFKIVFHKR"
     gene            complement(17466..19334)
                     /locus_tag="ONT85_001704"
     CDS             complement(17466..19334)
                     /locus_tag="ONT85_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2378678.1"
                     /translation="MISIIWFLFFFIFFSYLKVIKIDSFLVYHTSAMKNLTQNLLKKD
                     NSSILKIELKKENNKSMKISKKAFLLVVLILLSTLYSVNFMRNAQEIYTTGDLSFHLS
                     RIKGLSSIFEGPINYTTFNNYGDGLNYFYPFLTIIPAVVFYGISNNLILSYVLYIWLL
                     NICTILISFYYGEKFFKKIDAAFLFSCLYTFATYRTVDIYYRSAIAEAIALTIIPAVL
                     YYAYGLIFNREKKVIALSVSISLLVYTHVLTTLLCSALIACMIIVRLFLSKQIKNDIK
                     FVVVNLVKAVFLTCALTSYFWYPMLQQMNYQEIHSPFKTDLQLEALSISDSIIQALNN
                     DITTYTMGLLGLVALIFPLFILKEMEKKEKIIYVGVVSTWVCVTNLFPWFLFQKTPIS
                     LLQFPWRILGIQIFFSSMIITLVFMKKTMNKKYSWLIIGATVVFLLVTSIAAKENYAK
                     VIQSKHGHIVINEETLPRYTTSDMGGLYDYAPTEAIKERPHLEKHEVKVGESWEKGKY
                     KAADNNITYTVASNKKQKVTVPVYAYLGTQVKVNGKVVNDSYKKHGLVNVDVNAGENK
                     IEITTKYTPTALMALWISILTYCLVVYSSFKRFAPNKLKDSRYVTKKEINKKNENK"
     gene            19300..20937
                     /locus_tag="ONT85_001705"
     CDS             19300..20937
                     /locus_tag="ONT85_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2378679.1"
                     /translation="MKKNKNQIIDIIFMLFLSFAYVIPLFMSKGIYHSVNQDTYFHLS
                     RIIGLDNVWSSPVNFNNFDHHGTMMNIFYPWLTLYPAFLFYKMMGNLVLSYNIYYFFI
                     TFLTMVVSYFSMKQIKNNRYISLLFSIIYTFSAYRAIDIFHRASLGEAVALTFLPLIL
                     MGCYEIYIRDYQKWYWLSIGMTLVVYTHLLSVAMVSVFIGGTLFLSFYFWDQKIARLL
                     SLLKATVLTFFLSAGFLIPFIQQSRAQELKVPLGKELSGMAPSDMLTHILNNNYNNYT
                     IGLFLFLGLIGAFIFIKKLTADDLFIFFLGVFVLFCSTNLFPWQLFNHTPVKSLQFVW
                     RLNGFSSLFIAYTMSIVIYYIFSTKNNSWKIMVFTFLALILHLSGVSNSIHANKDSLT
                     LIAPEDAVAIAENYNHTDYANKESIYHPDMINNDQYLLGNQEINPTTHFMSNKLTIEL
                     DNSNNKNTVLTTPIYRYKGQVASINGKLVQTKLSKFGTTELTIPPGINKVVITYQYTK
                     LAIASRYLSIVTLILFLLYRFTFSKQKQPRREVQHSH"
     gene            complement(21082..21822)
                     /gene="truA"
                     /locus_tag="ONT85_001706"
     CDS             complement(21082..21822)
                     /gene="truA"
                     /locus_tag="ONT85_001706"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HCR2378680.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(22013..22648)
                     /locus_tag="ONT85_001707"
     CDS             complement(22013..22648)
                     /locus_tag="ONT85_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HCR2378681.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIARLSILHLKQ"
     gene            complement(22795..24036)
                     /gene="sstT"
                     /locus_tag="ONT85_001708"
     CDS             complement(22795..24036)
                     /gene="sstT"
                     /locus_tag="ONT85_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HCR2378682.1"
                     /translation="MLEKFLDMSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSC
                     LKAIAPILVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPIT
                     LQETVSEKAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSEST
                     KELVDQISIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLF
                     VALVVYPFLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKE
                     SYSISIPLGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGV
                     AGGSLLLIPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELG
                     ARKRSGEKIKLKDCLDGLSER"
     gene            24234..25232
                     /locus_tag="ONT85_001709"
     CDS             24234..25232
                     /locus_tag="ONT85_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2378683.1"
                     /translation="MFLSNFFVRCLFLKKIKKESYGFLTIFVACIMMVLQRILISAVT
                     IPTYATNPGRFYIYTFLQTSVVIGANLIPLYMGYQYQKIARLKVLHYLSRFAFIFLIA
                     TLVCNIFFYVQAGSLNIRDYWLILTPISQNYFTYAVSCTLLFCSIPYTVGFLNKLSKT
                     TIKYLLLFLTIGLVGCSTLFNKDPFALQNGQSILWIFYLFLMGYGLKEWNWKEKVRYK
                     LPQVFISLIVLFGLIILMTQISLIIRGNTDTALRFSKPYSLFAMYYTFSSFLFLEMLS
                     DKLGIKGRGNFLAVFLIVTQVICNFPLVSYRVSTFMKREFPNSGKAWLINIGQFFI"
     gene            25245..27263
                     /locus_tag="ONT85_001710"
     CDS             25245..27263
                     /locus_tag="ONT85_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HCR2378684.1"
                     /translation="MSLLVIFLFYIQKIRFIHRFIEYFAFDSIEQLTQRWKKRIHWLF
                     VHKSYFLVAAFFYCFTFVQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFF
                     FLLLLISNRFWYALTATLIINLLLTISTVIKMEMREEPVFPSDLKMLTGLSELLSMVS
                     PVLLIVGGLILLLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHK
                     NSPSFLLFNLFKVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMK
                     KYNTKADEINQTRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTG
                     GLMLSVGYGGGTANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIA
                     IHPFTAALYRRKQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQ
                     SNTETSQFIQLSTMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAV
                     KKFIQEIDKIDKPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAAT
                     LNKEVVAPNNFGAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVF
                     VTEDSQIIQEKNLTEKQKEILEDYRLIQYDLVAGNQYSATWATQKIKD"
BASE COUNT         8751 a         5256 c         4441 g         8822 t
ORIGIN      
        1 aattatctgt tttccaaatt ttataacata aaaaaccttt tttaacggca tttatatgct
       61 ttttcagaaa ttaactgatg atttcaaaaa gaaaacatgt cataatattt cccatattgt
      121 gcaaaaaaat acaaggcgga ggctctacta tgattgaaga atatattgag aaaaatattt
      181 taagacagct gtttttatgt gggcaattct atgtaaataa agaagtcaat ctagaaaagc
      241 tctccaatct cctgcatgtt tgcaaaacca ctttactcaa tgatatcaat aacataaaaa
      301 aagaatttga agaacagatc gcttatacac atcgggaaaa ggaccgttac actttatatt
      361 tttcagaaca tatacctcga tgcaagatca tgcagcaatt gtctcaaaat tcgttgtttt
      421 taaaaacttg tctgctttat ttggaagaag atgaacctga ttatcttcaa ttaactgaat
      481 gtgaattcat ctctgtcagc aaagcttatt cattaaaaaa acaagtatta gcttacttta
      541 atgactgcgg gatcgaaatc gaccgctact cccctcgatt cactgaaatg gaaagacgtc
      601 tgctgcttct taatgtgtcg tatcgtttag gcggttttaa ttcttgggag ctacctgaat
      661 cgttttttga acgtgccgat cgtttcattg aatccgtaac ggaaaatagt ggcagatttt
      721 atgataaaga aaataaagaa atcttatcga ttggttttgc tatcagtttc ctacgccagc
      781 aagtgtgtgc tgtgacaata gattcaaaat ttatcgaaga aatcaaaaaa cgtcctattt
      841 acaattatgt cgaatcagca tgggaaaaca ctgatttcca aacctattat aaaaaagaag
      901 aattcgcctt tattttgaca ctattcaatc tttgtaatta tggtttccat tcttatcagt
      961 tgattgcgga agactttcaa cagctacacc aagtgtttat tgacaatact cccgaaataa
     1021 aagaactagt tgctaccttt gaatctcact tcaatcaaga attattcgga aaccaaccgt
     1081 tcgaacgtgc gctgattcat ctgatgcgtt cggcttggga taactatcaa ttattcatgc
     1141 cagaaaaatt ttatctttta aatgaggaac agacaaattt atataaagaa gtccagacaa
     1201 ttttttccag ctggtccagc cagttacctt atgatctacg gttgaatcct aattgcatgc
     1261 gggcttttgt gatcgaattg agcggaattc tccgtctgac aaaagaacat ctgactatct
     1321 atatcgtcac aaattcggat gttcattatc tgatttatcg ggaagccttg gaagctgtaa
     1381 ctactttcga ttttcaagtt gcaccaacaa tttattcaag tatatctgat ataaagaagt
     1441 atgcgcagca gtcctcaaac cgggtattat gtgaacgtac attatataca ccagatgcgg
     1501 tccaatatga aaatattatt cctatttcga tcaatacgat cgatcgagct atcatttcag
     1561 ctgtgcaaaa caaataaggg gctgtgacaa aaatcatgtc acagcctctt atttgttgaa
     1621 gatgcccatt tctgtaacaa tctcttgata cacgtcaggc tcacaacctc acttattttg
     1681 gacatctagg ggtccgatct ctttatttaa aaaataagaa atgatggttc ggataactac
     1741 caacgccgct agttggaaaa tatcctgcaa ggtaggtttg ataatcgatt cgatgatccc
     1801 agctgaaaca agaatttcca aacttagaag tatatagcct cccaacatct ttttaatagc
     1861 attgttctcg cttactcggt cgctttgcac ttttgtatgg acaaataaat atttcaaaaa
     1921 atccttgatt gatagacaaa caccccaaac caataaacag atagcaaaga tatcaagtcc
     1981 cagtacaagt ttttcgaaat atggaagaag aaactgcatc aactctgtcg tcatcctacc
     2041 tctcctcttc tttgtttttc tttgtcttct atttcctgat tcaagaaata agaaatgact
     2101 gttcgaatga taataataag tgcaagcttc aaaatatctg tccaagtcgg cttgataact
     2161 gactcgatga tatcagcaac gactagtact tccaagctta acaaaatata acttgctagc
     2221 agttttcttg tatctgcatt acgagtatta cgttctgcat agtttttctt gacatgagac
     2281 caatcaaaaa gtttgaatag aacaagaacg atcccgacaa ttaagatacc gatagaacaa
     2341 atatttaaaa tagcaatgat ccattctaga aattcaatta aatttgagcg aaacatatac
     2401 gctacctact ttcttttaat ttgttcctat tcttttattg taaaagaaag ttaccttgtt
     2461 cgtctattta aaagttgttc taatgataac ggttcgctta aatttttctt tttcactctc
     2521 ccatttttct ccctccatcc ataaatcgtg gaagtatcca aaacaacgta gcaagttaaa
     2581 ataaggctta gtccgtcatc agcttttcaa agaagcactg tctcggtgct ttttttgtac
     2641 ccaaatttct aaaaatagaa ccatacacct cctcaaatct tttcttctta cctaccctat
     2701 ttttatttac aggaaaatca gacacttctt cttttttgtc aaaatttcct ttttcttctc
     2761 gtcctatcat ttggcattct aataaaaacg aactatcctt ggatatagaa agagaaaaca
     2821 aggaggcgtt tttcatgagt aaaaaattaa tcaaagtcgg catcggttta ggtttgcttg
     2881 cccttggcgc tgcatatcta ggaaaaaaga caggactatt cgaagatgac agtcatttat
     2941 atgatgaatt cgaatcaatc tagcatccta tttagttgaa tcactacgga catacttgga
     3001 agacattgga ggaatcacaa tgagaaaaag taaagcaata atgattggtg ccggtattgc
     3061 caatatggca gcggctgttt atttgattca agaaggcaaa tggaaagggg atcagatcac
     3121 cttttattca ttggatgatc acggttcaaa tgatggtgca ccgaccgaca ctgtagtgga
     3181 tgaatattgg aataaaaacc atccattaga aaaccaaaaa ggctatattg ctcgaggcgg
     3241 tcgcatgctt aattaccgta cctatgtaga tttgatggat ctgttaagtc gaataccttc
     3301 tgttactgag ccagggatga ctgctgaaga agatacgaga gattttgatg caaaacaccg
     3361 aacctttgac aaagcacgtt tgatggaagg tggcaaagga atcattgatg gcggaaaact
     3421 gggattcaat aataaagacc gtattcttct gactaaactg attgctatgc ctgattcaga
     3481 agaagaaaaa ctagacaata tcactatcga agaatatttc aaagatgatc cacacttttt
     3541 tgaaacaaac ttctggttta tgtgggaaac aacatttgct ttcagaacac gtagttctgc
     3601 tcaagaattg cgtcgctata tgcatcaaat gatttatgaa ttcactcaaa tcgaacatct
     3661 agtgggcgtc aatcgaacac gttacaatca atacgaaagt attatgcttc ctttgatcga
     3721 gtacttgaaa gaacaaggat gcaaaatcat tttgaaccgc cgtgtcactg actgggaatt
     3781 caaagaaaca ccgatgcaag atgaaatcac tgtgactggt ttgaaaatta ccaatacact
     3841 gactcaagaa gaagaatacg ttgtggtcga tgaagataca gcagttattt tcactaatgg
     3901 gtccattaca gattcagcta ctttaggaga ttacgaaaca ccagctgtag aaaatatgga
     3961 ttatggtgca gcttctagtc tctggaaaaa agccagtgaa cgtttctaca acttgggaaa
     4021 tcctgacaaa ttctttgctg atcgagatgc aagcgaatgg gtcagcttta ccctgacgac
     4081 gaaaaaccat ttgttgttga atgaaatcac ccgtatcacg actcaagaac caggaaatgc
     4141 cttgaattca tttatttcaa ccacaccgat cacgccgtta ggccaaaaag atgtcaatat
     4201 gtctattgtc gtgcatcatc aaccgcattt tacgtctcag aaaccaaatg aaacagttat
     4261 ttggggttat ttcctttatc caagacgacg tggcgaattt gtggataaac agtatattaa
     4321 aatgaatgga aaagaaatgc tagaagaact gattggacaa ctttcaaaag tagatcctgg
     4381 accagtgaat attcgtgaaa aagaaacaga aattttcgac agcgtaataa ataatatccc
     4441 tgtctatatg ccttatgctt ccgcgttgtt caataatcgt gccaaaagcg atcgaccaaa
     4501 agtcattcct aaacattcga ccaatctggc ctttacagga gaatttgtcg agcagcctta
     4561 tcagatgatc tttactgaac aaagtgctgt acgttctgga gaaatcgctg ctttccattt
     4621 tgcgggtatt cctatgtccc gtcttgtcaa aacaccgcgt tacgacaaag atattcctac
     4681 tctagctcgt gccgcaaaaa aaatgtttga ataatcaaga ggttgtgaaa aagctgccct
     4741 gcatcaagta ataagaacaa ccaggctaaa acagcttctt atgtttttga tttggttgga
     4801 cttattaccg cagatgcaag cttttgaaca ccgtttacca agaggttatg aaaaagctgc
     4861 cctgcatcaa gtaataagag cagccaagct aaaacagctt cttatgtttt tgacttggtt
     4921 agacttatta ccgcagatgc aagcttttga acaccgttta ccaagaggtt atgaaaaagg
     4981 cgtttagttt cgagtatgaa gaaaaatttt gtgaaaatgg ttcctcacgt ttttgcataa
     5041 atttgactta ataccgagaa actgatccct tctgtcataa cctcttttta tttatgtctg
     5101 tttctttttg aaaacaacta ttttttcaac gtttactctc ttgccaatcc aacatcatct
     5161 aacggataca actatttcaa tgattaccat tacactaaaa aatcggaaac acacctctcc
     5221 gaacacaaat acataagact atagaatgtt tcatttaaga aaaaaagcgt ttcattatca
     5281 aaaaggtaag atctccttta aaatgaaatt atctgtagta ctaataatgc agacaatttt
     5341 atgaatctga aaggagcatc tccaatgaaa gcagtcactt ggcaaggaaa acagaaaatg
     5401 gaaatcagaa acgtagatga tccaaaaata ttagaaccaa cagatgcaat catccgtatc
     5461 acagcaacag ccatttgcgg ctccgatctg catctttacc atcatggtga agcagttttg
     5521 gaaaaagggt atgttgtagg acacgaacca atgggaatcg ttgaagaagt gggatctcaa
     5581 gttcaaacat tgaaaaaagg gcaacgtgtg gttatccctt ttaatattag ttgtggtcat
     5641 tgtcatttct gcctgaatca catggaaagc caatgtgatg aatcaaatcc agatcagtta
     5701 tatggcgggc ttttcggatt tggcaagttg aacggtaatc attggggtgg tcaagcggaa
     5761 tatctaagag ttccttatgc agactctact tcatttgttg taccagataa tgatcttcct
     5821 gatgaaaaag ttttatttct atcagatatt ttgcccacag cttattggag cgttgaaaac
     5881 gcaggagtaa aaaaaggcga tacagttgtg attctaggtt ctggtccagt cggtctattc
     5941 gctcaaaaat tcgctgttat ggctggagca gagcgcgtaa tcgcagtaga tccagtacag
     6001 caccgtctag ataaagcggc taattacaat ggggttgaaa cttatttctt agaagacacg
     6061 gcacaagctg gaacagaatt gtatgaactg gctaaaggtg gcgcagatgt cattatcgat
     6121 tgtgtaggaa tggacggatt agagcctgtc aaagaaaaag cgaaaaattt agtaagctta
     6181 caaagcggca caatctctcc attacagatg gcatcgcaag ctgtcaaaaa atttggaaca
     6241 gtccaagtaa ctggtgttta tatgactcca gcttcttctt acccgctgca agaatttttt
     6301 atgcgggata tcgctgtcaa gcatggacag gcaccaatta tccatctaat gccaaaaatt
     6361 tacgcaatga ttgcagaagg actattcgat cctacacaaa tcattactca ttcgctgcca
     6421 ctatcagaag cagcacaagc atatgagatc tttgacaaaa aagaagataa aaatataaaa
     6481 gttgttttga aaccttgaat tcagtaaaaa atacaggtga ttttctttat gcccaaaatc
     6541 aaacgaaata cccatgagat cgaaatagga aaagaagaag atatcgaaat acgaggacgt
     6601 cgcttccgct tctatttcca aaatcgttac acattgatct cactggctgt cgatttatta
     6661 accgggattt tctatatttt tggaagcctt gcttccttaa ctaaaatccc cgatcgatat
     6721 ggcacctatt tttatttggc tggtgctatt ttcttgacga ttcgtcctat tctcagaatc
     6781 gtaaggaaca tttttattta cgacgaaaaa aaagcaatga agagaacaag caaaaaaaaa
     6841 gaggcatgag aatttttcaa atctcatgcc tcttttttca ccctatcgta tcactttgat
     6901 acagacgagc ggtcgtccca ctttcttcct tgaccattgt caaaaatgtc cactcgtatc
     6961 gttcataaaa cgtggtatga tctgtaatta agtaaagttt ttcgtaccca agttgatgca
     7021 tttcttttct gatttccgct aatatccgct tggctatccc ctgcttacgc ttttccggtt
     7081 caacaaacag ggcacaaaga tttggtgtca gatcagggcg gttatggaag tcgttttcaa
     7141 tgacgcccgc tcctccgacg atggtttctt tttcatctaa gaccacatac cattgaggaa
     7201 tactttcctt attagcgatt ccagtacgaa tactttcctc atacacaaca gcaggaactc
     7261 cccattttga tgaaaaccaa ttcgcgcttt gttttactaa ttctgggtgt tctcttaatt
     7321 tgatccagtt atatgtaacc aatgtttaaa cctccgacta aaataaaacg tttacaaaaa
     7381 ttatacaagt ctttatgaaa attagcaatg acaacaatag tgatgtaaaa aaaaaaacgg
     7441 aaaacaatca cttgttctcc gttttttgat taatcaagca tctaaagctt cttttcttct
     7501 ttcttggatc gtagctacct tgacgaacgg tagatagatc aaaatagaaa gaaccaaatt
     7561 gacagcggct agtacaccac ctgcaatact ttttgttgcc aatacaccgc caataattgg
     7621 tggagttacc catggaggca taacggtagc tgctggcact aaacctgtag aagtcgcaaa
     7681 gtaagcaaca ctaactaaga ccattggtgt caaaataaat ggaatgaaca tgatcgggtt
     7741 caacacgata ggtaacccaa acatcgttgg ttcattaata ttgaagacac ctggcgcaat
     7801 cgacaagttt gttacaacca tatatggttt gtttttacgt cctactaaat aaatagctaa
     7861 cagtaaacct aaagttgcac ctgttcctcc taagttgaca aagctgtcaa agaaaggttt
     7921 gttcacgata aacggtgcgc tatgtccagc tgcgattgca ttaacgtttg cttcaatggc
     7981 tggcgcattg attgtttgca tcaaaggatc gaccatattc gctccgtgta aaccaaagaa
     8041 ccataagaat ggtgtgataa atgcaagcag taaggcagat ggataagagt ttgcaagtcc
     8101 cataaatggt tcttggacca ctgtatagaa agaacctacg atatcatcaa caccgaatgc
     8161 agcaaaaatt gcagcaacta aagcaaacgc agagatagtg atcattgctg gtagtaatga
     8221 agcaaatgct tttgcaactg ccggtggcac accatcaggc attttgatga tcaatttttc
     8281 atttccgctt aaacgtacaa agatttctgt tgaaataatc ccaacaataa tagcaataaa
     8341 caaaccgttt gagctcatac ctaatgctcc gcctaaagcg aagaaagagc ctaaagaaat
     8401 cacacctgcg gaaacaccgt cttttcggta gccgttagct aaatgataag caagcaagaa
     8461 agcaatcaag acagaaagaa tggcaaatgt accgttccaa gcagcagccc cgaatccttt
     8521 ccatgattca ccaccaaaaa ttgagttcat caattcttgg aacccgggaa tcggtaagtt
     8581 attgatcatt actgctaatg ctcctaagat cattaacggc atactcacca taaatgagtc
     8641 ccgaatcgca actaaatgcc gttgtgcacc aatttttgat gctacaggta taaaatgttt
     8701 ctccataaat gcaacaaatt tatccataaa tcttccctct tttctttact gtagattacg
     8761 cgttcaatcg acgatacaga ttaatgatct cttttgctaa atctgtaaaa gctatagatg
     8821 tcatcagatg atcttgactg tggaccgtca aaagcgtgac ttcaacgtga tttccttgtg
     8881 cttcttgtgt cagcatctct gtttgagagt gatgtgcttg gactaaagat tcctctgcat
     8941 ctttgatttt ttcatctgct aaagtgaaat cacctttttt cgcagcagca atcgcttcca
     9001 ttgcatcgct ttttgcattt ccgccaaaca tgatcagacc catgatggct tctagatttt
     9061 tttgatcttc catttattgt atcctccgat aatttttatt atttgtttat tagtttttct
     9121 gcttggtcga gtactttttc accattcatc atgccatagt cagccatatt gattacatct
     9181 aaaggaatgc ctttaggtgc tagcttttca gcaaattgat tttgcataaa gcgtacttgc
     9241 ggtcctaaaa gtaaaacgtc tacattttta gattctagtt gattatccgc atctgaagca
     9301 gatacagcaa agatgtctgc ttccattcct tttgcttctg ctgccttttg catttttgtg
     9361 actaggagac ttgtactcat tccagctgaa catacaagca tgattgtttt cttttccatt
     9421 ttaaacacgt tcctttctat acttacactt gtatataagc aagtagcgtg ccaactttaa
     9481 aagcgcttta aaaagttgat atcatcacat ttaaggtcta taccaagttg catacacact
     9541 aaaaaagaaa gcgcacacta aacacacaaa ttaattttta ttaccttttt tcttttaacg
     9601 tgcacaaaaa aagttgtgac acaaaacttg tcacaacttt tttcaaataa acggtattca
     9661 aaagcttgca tctgcggtaa taagtccaac caagttaaaa acataagatg ttgtttttac
     9721 tttgctattc ttattacttg atgcaggaca gctttttcac aacctctcaa taaacggtgt
     9781 tcaatcagct gaccctgggt attagctcaa aacgctggat tcacaacctc ttttatttca
     9841 aataaccagt aaatacaatg aacatctaca aaaaacttct atttatttat agatttcgtc
     9901 aggcaaatcc aattctctct tatcaatcaa agtctgatac cacgaagcag attttttcaa
     9961 tgttctgctt cgttcatcgt tcaattcgat tcgaactaat ccatatcgat tcttaaatgc
    10021 attcattggt gacacacagt cagtgaatgc ccataacata tagccttcgc aatttgcgcc
    10081 ttcttctgtt gctttgatca agtaatgcaa atgctgtccg ataaattcga tacggtaatc
    10141 atcttgcacg attcctttct catccatata ctgttcttca tattctcttc ccataccgtt
    10201 ttctgttatc agccaaggga tattatgata attttctttt agatacatgg ctatatcata
    10261 catgattttc ggatagattt cccatcccct ggaattattc attttcttcc caggtaaatc
    10321 aaagacttca taatatcgtt caggatggaa aggtgtctct tcattccatt gataacgagg
    10381 ggcgcgaacg cgtttaggat aatattgatt gattccaagg aaatctactg tatgatgctg
    10441 aatcgtttct aaatcctctg ttgcaggatc gaaataaatc gattgttttt gacacaactc
    10501 caggaactct tctgtgtagt atccttttac catcggatca aaaaatactc gattataaaa
    10561 caaatcatac atataagcag ctttttggtc ttctggagaa ttggatcgag catagaccat
    10621 ttcaggattt aaaatacagc caatccttcc accataattt ccgctgcggt aaatctctac
    10681 tgctttagcc gttgctaaaa ctttatgata attccacatc atccactttt ttgtgttttg
    10741 ttcatgcggc cagcgaacag catccaaata gcaacgcgtt tggggaacga ttggttcgtt
    10801 gaatgtaaac cagtttttta ccctatcacc gtaacgatca aaggcaattt gagcatactt
    10861 agcataaaaa tcaactactt ttcttgaaga ccagccatca tatttttcca aaagaaaagc
    10921 tgggacttca taatgttcca aacaaatcat cggttctacg tctgcttcga tcaaagagtc
    10981 gatcatttca cttatatgct gagcatattc ttcatctact atacagttct cataatcagt
    11041 aaaaaaacga gcccaattga tagaagtccg ataatgtgtc agatggatgg atttcatcag
    11101 atccacatcc tgctggtatt gttccataaa attagttgct acggctgggc catacccatt
    11161 atgccaaaca aaatgttcat ttttatacca gctatctata aacgaatcct gtccttcttt
    11221 ttttcctttc caaccttccg tttgccaagc agaagatgca gcaccaagaa taaaattttt
    11281 tggaatagta attttcatga tttcctcctg ttttttagat cccatcattt tccacacgat
    11341 ttgctacttt tacaaatggc agatagatca aaatagaaac aataatacaa acaagttgcg
    11401 taataactgc accgatattt cctgctgttg ccagataagc attgacgatc ggcggcattg
    11461 gccaagggac cattacaact gctttcgccg caaatcctaa agcggttgca aaataaccaa
    11521 tcgttccagt gataagaggc gttaagataa acggaatagc taaaattgga ttcagcatga
    11581 tcggcatacc aaaaataact ggttcattga tgttgaagat cccaggagca agggataact
    11641 tggtgatttg tttcatatct tcccgttttc caactaacaa tactgcaatc agtaaaccga
    11701 tcgtgactcc agacccgccc atactcatat acatatccca aaatggcata gtaataatgt
    11761 tcggtacttc ttttccagct tcaaaagctt ccgtatttac agcaattgcc gctaaaagca
    11821 acggttcccg tatcggcttc accatttgat ttccatggat accaatcacc caaaagactt
    11881 gcgcaataaa catcaatagc aaaatacccg gtaacccttg tacgacacct tctaatgggc
    11941 gttgtacgac attgtaaatg atatcataaa ggtacattcc tgtaagttct ttgatgataa
    12001 atcccgcagc cgcgataccc gatacagtca gaatcgtcgg gaataaagca gaaaaactag
    12061 cagaaacatt cgttggcacg gtatcaggca ttctcagctg tagtttcttc tgtttcctca
    12121 accagcaata aagttcaata gaaaggatgg agaccaacat tcccaaaaat aaccctttcg
    12181 tatctgtata ttgtttagcc aatacgtttt ctactataac agtctgattt tcattcacta
    12241 attcataaac agttggatta acagataaat aagacatcac tgcgatgata ccagggaata
    12301 caccatccac tttattcagc ttggctaatt cgatccctat cagaaaaaca gcatagatgg
    12361 tcaagaaact tagtgtgaca tagctgatgg cagatgcgat aggctttaat tcttctaaaa
    12421 aacgcaagaa acttatctgt gctaagccat tttccgagtc aaagaccatt gctgaaaaca
    12481 atgtgccaaa agcacccgta ataattaccg gaatcaatgc agcaaatgcg ttttttatca
    12541 ccatgatata ccttaaatta ttgattctcg ttgccacgtt ccctaaaaca ttggctaatt
    12601 tatccataaa actcattcca gatcccctca ttcctgcttc ttctccttga cgtcatctct
    12661 ttttgtctat tagtcagcgc ttacatttcg atgaacagaa actatttact cctctttttt
    12721 accaaatata aaacaaaaat tcatctgatg tatttatcat aaaatacatc agatgaactg
    12781 tcaattagtt tctttttctc ctattatttt ctgtaattct ctccgattta ttgccatatg
    12841 gacagtcatt gcatcaaacg cagcaagtgc attccgcctt tcgatggcct gaacgatctc
    12901 tgtatgaata tcgatcgtct cgattttcag atgctggata tcataatttt tattaaacaa
    12961 agcaattgat tcattaatga ttgggataat atgcagcaag gctacgtttc cgctagcttc
    13021 tgcaatcatc gtatgaaaat cgatgtctaa ttgaacatgt tcttgtgtgc cttctcgaaa
    13081 agaagtttca atcttttgcg ttaatttttt catttggacg atttgattct tcgttgcata
    13141 ttccgctgcc aaacctgcca tcttaggctc caataaatat ctcagttcaa ataagtctcg
    13201 tatcatccga tcctgatctt caatcaaaga aaaaccgagt gggtcattgg caatccctcg
    13261 cttatcactg acaaatgtcc ccgctccttg ccgaacttca aggatattcc tggaagctag
    13321 agcccgcaca gcttctcgca ccgtgcttcg ccccacctct aaataatcgg acaattcata
    13381 ttcattcggc agtttatctc ctacttgcaa ctgcatttcc tttaaatatc taaggatctt
    13441 gtcagtagtt acttctacta gggttttatt ttcttccaaa ccacatacct ccttttcatc
    13501 tgatgtattt tttcgtaatt attataacac gtttgaatca tctgatgatt ttttgtatac
    13561 aaaattgatt aatccgttca tagacataca aacgaagaag atatttacca taaaaaagac
    13621 taggataaaa ttcactccta gccttcgatc atttttattt tgaacgacaa aaaatatgat
    13681 gttcaataac agacgatctt cattcgattc ttgatgtcat cttttgtttt ttttaatata
    13741 tggataaaaa gtttgattac atcgtttcta ttctagaatt tggtcgattc tcaattcttc
    13801 ttctggtgag aaatctaaat tatccatcat tcgaacactt tcttgtaact gacttaaccg
    13861 acttgctccg accagtacac ttgtaacaga tttttgtcgc aaattccatg caagggccat
    13921 ttgcgctaat gattgtcctc gtgatacggc gattgtttgc agtgctttga ttttttccag
    13981 agttgatcca acttgttctt ctgaaaggaa tggtatttcc ttacgatgtg ctctagaatc
    14041 ttcaggaata ccatgtagat aacgatttgt cagcaaccct tgagcaagcg gactgaacgt
    14101 gattgcccct agcccttctt cttctaatac tggagataga tcgtcttcta gtagtgcacg
    14161 agaaaacata ttgtagcgca tctgatgaat aatgaacggt gcttcctttc tttttaaaat
    14221 ttcagtcatt ttttttgtat cttctccatt atagttggat ataccaatat ataatgcttt
    14281 tccttgtctt acaagttgca tcaaagcttc tgctgtttct tccaaaggtg tatcagggtc
    14341 tgggcgatga tgatagaaga tgtcaacata atctagcccc attctttgga gactttggtc
    14401 acaacttgaa atgatggatt tacgagagcc ccattcccca tatggccctt cccacatatg
    14461 ataacctgct ttgctagaaa taatcagttc atcacggtag gcttgaaaat cttctctaaa
    14521 aatacgccca aagtttttct ctgcagctcc agcagggcct ccataattat tcgcaaggtc
    14581 gaaatgggta attcccatgt cgaatgcgcc tcttaaaatc tctctttgat tatggatagg
    14641 atcataatca ccaaaatttt gccataatcc gagagataaa atcggtaatt tcaaacctga
    14701 atgaccggat ttacgatatt ccattttttc ataacgtgca ctatctgcaa tatacaattt
    14761 ttctcctcct catcaaatct ttttaaaacg ctttcttttt tctattttta gtttactaca
    14821 ttttaaagaa aaggagttaa tcaaaatgat tttttcgtat atttttttgc catttgtttt
    14881 ttactcgttg atagatcaga aattgattac gtgcttgctg gattttttta taacgtttcg
    14941 gttgtttttt gtaaaattgc tgatgatagt tttctgctgg ccaaaacgta ccggctggtt
    15001 cgatttttgt gacgattggt tggtggaaag ttcctgaact gtctagtttc tgttttgatt
    15061 gttccgctaa ttctttttgt cgttcgactt gataaaaaat cactggacga tattgcttcc
    15121 ctctatcttg aaattgtcca aatgtatctg tgggatcagt aatttgccaa tagattgtca
    15181 gcaactcgct ataacttatt tctcttgtgt cataaatgat ctccacagct tccacatgac
    15241 ctgtgtaacc accacttact tgatcataat ttggtttttc aacatgtccg cccgtgtatc
    15301 ctgacaaaac agataggatc ccttttttcg attcaaacgg ctcgaccata caccagaaac
    15361 atccccctgc aaatactgca cgttcttgat agtcagcatc ttgcatggca tgcttggaaa
    15421 agtcatattt ttgctcctta tcaaatcctt ccgtaatcaa taaataaaag tccattacat
    15481 caggcgtcag attcattcgg atagctaatg gacgtagtgt tgcttctagt tttgataact
    15541 gttctttaac ggatatattt tctcttatgt cattctttgt ctgaatcaat acttttcgtt
    15601 cccaatctcg tgtattcgga ttcaaaatca gattataaag tgtttgaatg aatatttgtt
    15661 cctcatccat agattctcct cctttccgaa gaagacaagc tagtgacttt agctgtaatt
    15721 tatcataaag tgaggaaata agcgattact tcatccccct acagattttt cgtataaaaa
    15781 agagagaatc cttatcgaca gattctcctt tttaatcaat ttattttata aatgaaaagt
    15841 taatttacct tttatggaaa actattttaa aaaataaaca taaatatgca gttaatgtaa
    15901 caagataaga aataatagtc gttactgtat aacttgtgct tactttaata tcagcaactc
    15961 ctttgactcc gcttatttgt accaatccat tagcattcac ctgctccttt acaggttgat
    16021 gatttatttc tacattcgtt cctaaataag catatacagg aagtgtgatt acttggctct
    16081 tctcattagt tactctatat atgatagaac taccagttgt tttataagga aggtttaccc
    16141 attgactgcc aatctttgct tcgtgtttta ttatatgctc cttattctcc aatgaagcga
    16201 taggagaata atcatataaa aaataatcca ttcctttagt ggtatacgcc tctactgttt
    16261 ctttgtttac aacaatatgt cccggctgag aaattatttt ttgattgaaa ttttttttac
    16321 tagaaacagt taaagtgatt aataacaaaa cagtcataaa taaataaaat agttctcttg
    16381 tcttattgta tcttctattt ttgaaaaata tcatcgttaa tatcagagaa gagaatatta
    16441 tttgcaagct taagatccgc caaggaaatt gtaacaattt ggctggcgta ttttgcagaa
    16501 gataccatgg caccaaacta gttgtagcta gccaagtcaa acacgtacca taataaatct
    16561 ttttttctat gtttgttaat tttttaaata aaatcagggg aatacataaa gataacagac
    16621 caactagtcc cattgagtaa gtagttaagt cgttgttcat agcttcagtc agactatcga
    16681 acacattaga tgcatgagca tataaatttg tcacgctagg tttgttgatt ttttggtaga
    16741 gcatttgttc aaacatcgga taccaaaaag cactcgtcag aaccaatgtc attcctgccg
    16801 cactaaataa ttttttaaat atagcaataa aatctgcatt cttgaaacct ttagaaacca
    16861 aacgaataaa aataaaaatg ataattaatg ctgtactcat taatgttgat agtacatggc
    16921 tgtacactag caaactcata ctcaatgcta gctttacaga cggatacttt tcttcttttc
    16981 cagagatgat ttgataagca taaaataata cagggataac aagtgttatt gcgatagctt
    17041 cggcaattgc ggaacgatag tagatgtcta ctgtccgata agcagagaaa atatagagac
    17101 aactaaataa aaatgccgct tttttttgtt ttaaaaaccg ctcgccataa tgataagcta
    17161 cgagtgttgt acacaagttc aataaccaaa cgtaaataat atagcttaca atcaaatttt
    17221 ttgtcatcca atataacatt acagctggaa aaaaagtcag aaagggataa aaataattta
    17281 caccatatcc agtataattg aatgtctcat aattaatcgg actggtaaaa atagtcgata
    17341 accccttgat tcgagataag tgaaaagaaa gatctccagt ggtataaata ctctgtgtat
    17401 ttttgaaaaa gatgaaagaa tatatactac taactataat taatccactc aatattacta
    17461 cttttttatt tattttcatt cttcttgttt atctcctttt tagtcacata tcgagaatct
    17521 ttcaacttat taggtgcaaa tcgtttaaaa cttgaataca ccactaaaca ataagtcaga
    17581 atactgatcc ataaagccat gagagcagtt ggagtatatt ttgttgtaat ttctatctta
    17641 ttttctccag catttacatc cacattcact aagccatgtt ttttataaga gtcatttacg
    17701 actttaccgt ttacttttac ctgtgtacct aaataagcat atactggtac tgtcactttt
    17761 tgctttttgt tgcttgccac tgtgtaagta atgttattat ctgcagcttt atacttccct
    17821 ttttcccacg attctcctac ttttacttca tgtttttcta agtgaggcct ttcttttatc
    17881 gcttctgttg gggcgtaatc gtataatcct cccatatctg atgttgtata acgtgggagt
    17941 gtttcttcat tgataacaat atgaccgtgt tttgattgga taacttttgc gtaattttct
    18001 tttgctgcta tagacgtgac gagtagaaac accacagtag cgccaatgat taaccatgaa
    18061 tattttttat tcatagtctt tttcataaaa acaagcgtta tgatcattga agaaaaaaag
    18121 atttggatac ctaagatacg ccaaggaaac tggagtaagc ttatcggtgt tttttgaaac
    18181 aaaaaccatg gaaataaatt agttacgcat acccatgtag aaactacgcc aacatagatg
    18241 attttttctt ttttctccat ttctttcagt atgaataaag gaaaaatcaa cgcaaccaat
    18301 cctaataacc ccatagtgta tgttgtaata tcgttattta acgcttgaat gatactatct
    18361 gaaatagaaa gtgcttctag ctgcaaatct gttttgaagg gactgtgtat ttcttgataa
    18421 ttcatctgct gcaacatagg ataccaaaaa tagctagtta atgcacaagt caaaaaaaca
    18481 gcctttacta aattcacgac cacaaatttt atatcgtttt ttatttgctt gcttaaaaac
    18541 aaacgaacta taatcataca agcaatcaat gcactacaca atagtgtagt taagacatga
    18601 gtatacacaa gtagacttat actgacagat aaagcgatga cttttttttc tctattgaat
    18661 atcaatccat aagcataata taaaacagct gggataatcg taagagcgat tgcttctgca
    18721 attgcggatc gataatagat atcaactgtt cgataagttg caaaagtata taaacaacta
    18781 aataaaaaag cagcatctat ctttttaaaa aacttttctc cataataaaa actaatcaga
    18841 atcgtacaaa tatttaataa ccaaatatat agaacataac ttaatattaa gttatttgaa
    18901 attccataaa aaacgactgc aggaattatt gtaagaaaag gataaaaata gttcagtcca
    18961 tctccataat tgttaaatgt tgtatagttg attggccctt caaaaataga agataaccct
    19021 ttgattcgtg aaagatgaaa agataaatca cctgttgtat aaatttcttg agcatttctc
    19081 ataaaattta ctgaatataa agtactaagt aagataagaa ctactaaaag aaatgctttt
    19141 ttacttattt tcatagattt gttgttctcc ttttttaatt ctattttcag tatactagaa
    19201 ttgtcttttt ttaataagtt ttgcgttaaa tttttcatag cactagtatg atatactaga
    19261 aaagaatcaa tttttattac ttttaaatag gagaaaaaaa tgaaaaaaaa caaaaaccaa
    19321 ataatagata tcatttttat gttatttcta agttttgctt acgtcatccc tctattcatg
    19381 tctaagggaa tctatcatag tgtcaatcag gatacttact ttcatttatc tcgaatcata
    19441 ggactagata atgtatggag ttctccagta aacttcaata acttcgatca tcatggaaca
    19501 atgatgaata ttttttatcc atggcttact ctttatccag cttttttatt ttataaaatg
    19561 atgggaaatc ttgttttaag ttataatatc tactattttt ttatcacttt tttaacaatg
    19621 gtcgtttctt atttttctat gaaacaaata aaaaacaatc gttatatttc gctgttattt
    19681 tcgattattt atacattttc cgcctatcgt gccattgaca tattccatcg tgcttcattg
    19741 ggagaagctg tcgctttaac atttttgcct ttgatactaa tggggtgtta cgaaatttat
    19801 attagagatt accaaaaatg gtactggttg tctattggca tgacgctagt agtgtatact
    19861 catttacttt ctgtcgctat ggtttctgta tttattggag gaacgttgtt tctttctttt
    19921 tatttttggg accaaaaaat cgctagactc ttaagtcttt taaaagctac cgtcctcacc
    19981 ttttttttaa gtgcgggatt cttaattcct tttattcaac aatctagagc acaagaattg
    20041 aaagtcccac tgggaaaaga attaagtggt atggctccta gtgatatgct gacacatatt
    20101 ttaaataata actataacaa ctatactatt ggattattct tatttctcgg attgattggt
    20161 gcttttattt ttataaaaaa attaactgct gatgatttat ttattttctt tcttggtgta
    20221 tttgttcttt tttgttcaac caatttattt ccttggcaac tttttaacca tacaccagtt
    20281 aagagtctac aatttgtctg gcggttaaat ggtttttctt ctttatttat agcctatacg
    20341 atgagtatag tgatctatta tattttttct actaaaaata attcatggaa aataatggtc
    20401 tttacgtttc ttgcactaat attgcatctc tcaggagtaa gcaattctat ccatgctaac
    20461 aaagattcat taacacttat agcacctgaa gacgctgttg ctatagctga aaattacaat
    20521 catactgatt atgcaaacaa agagtcaatc tatcatcctg atatgattaa taatgatcaa
    20581 tatttactag gtaaccagga aataaatcca acaactcatt ttatgagtaa caaacttaca
    20641 attgaattag ataattcgaa taataaaaat actgtcttga cgacacctat ttatcgttat
    20701 aaagggcaag tggcttctat taatggcaaa ctagtacaaa caaaattatc taagtttggc
    20761 acaactgaat taactattcc tccaggtata aataaagtcg ttattactta tcagtacaca
    20821 aaactagcaa ttgcttcaag atatttatct atcgttactt tgattttatt tttactttat
    20881 cgatttactt tttctaaaca aaaacaaccg agaagagaag tacaacattc tcactagatt
    20941 tttatttaag aaagactttt tagctgtaca cataaacagg ctaaaaagtc tttttttata
    21001 tttctttaat ttgcttcata cattatttaa catcgatatt tatactgtga aacatgcgtt
    21061 tcatccatta tcaaagtatt tttatcgata ataaacttca tccaaaaaca gaccttgtgc
    21121 agggacggtt tctcctgcat gttgccgcac tttgctttca aatatttcat cgattgtttc
    21181 tatcggcatg gcacctgtgc cgatttctag taatgtcccc atgataatcc gcaccatttt
    21241 atataagaat ccgtctccga caaaagtaaa atgaagcatt tctccctttc gttcgataga
    21301 taattcatca atcgtacgca ctgtggattt ttttgatttt ttcaatgcag aaaaaccaat
    21361 gaaatcatgt gtacccacta atttttcgca agctgcattc agcttgtcca tatccaactc
    21421 ttgagggaaa taaaagctgt ggtttcgctc aaatgctgaa ggaatcacgt tgttccaaac
    21481 ataatagctg tattttttgc ccaccgcatt atatcttgaa tggaagcgct caggcatttc
    21541 ttcgattctt ttgatgacaa tatcccgcgg aaggtaacga ttcataaagt cgatcatttc
    21601 tgccagtcca atttctgtgt ttgttttaaa atttgctact tgtcctcgcg catgggtgcc
    21661 agcatctgtt cttcctgatc caataatttc tatctcttca tttgtcatct gatgcaatac
    21721 tttttcgatt tttccttgaa tggttttatc gtcatctcca agtctttgcc atcctgaata
    21781 tctttttccg tcatattcga ttgtcaactt gatgtttctc atattttagt ggctcctctg
    21841 tcttccattt ggtcttctct ctaccttttt aagattgtag cacaaaatat agctccttgc
    21901 ttctctaaga cagaagctat acaagaaaac tgtggaaata tatatttcag aaaaacaaaa
    21961 aacagccacc ggatgaagta tccgctgact gttcaatttg acggctcaag ttttactgtt
    22021 tgagatgcaa gatactcaac ctggcaatgg gcacataatt aaagaaaaaa ctgattcttt
    22081 ggcaaaaacc tattttattc agtaatggcc aagaatttat agaaggaatt cttgccagtc
    22141 cataaatcgc cgcaaaaagc aaactgatca cagacagtcc aaggtataag tggcttacgt
    22201 ccatttgccc attttttcga tagatcaaga aaagaagaac tggaaacaat aaaaaaccac
    22261 tgtaacctaa acctgatccg atattatgga cccatgtcga gacattccac gaagattcat
    22321 gggtattgat actgaacaaa cctgtaaaga tacaatcacc tataccatag aacgcgatgg
    22381 ctgctgtaac tagggtagct aacacacgag atgtactaac aaatgcagca tacaatgcag
    22441 gcaatgacag gatgaaaaag atccctgaaa ccacagacca aataagaaac gcttttctta
    22501 caggactgga aacctctcca aatacactga tcacgctctt catttgatct aattcaggat
    22561 aatacaatcc taatacgtac ggagtcaaaa aatcactcag cacgcctaat aataaaaaga
    22621 aaaaaccaaa tttacgtaaa aaatccatga tacgcctcca ataacaatta cctatgcttt
    22681 taattgttat tataaacgac tttatcttga tttaacagaa acctctattt acacgaacat
    22741 ctatataact cattaaaaac tcgaatcatt ttaaaaatac taaatatcta acgttcaacg
    22801 ttcacttaat ccatctaaac aatctttcaa tttgattttt tccccacttc ttttacgcgc
    22861 gcctagctct cctattgcag taaacaacag atcacttgac gaatttaagg cagtttccat
    22921 tgaatcctgg ataacaccga taataaatcc aataccgacg atttgcatcg cgacatcatt
    22981 agagatacca aacaaactac atgctaatgg aatcaataac agtgaaccgc ccgcaacacc
    23041 tgaagctcca caagcagcta aagcagacaa aatacataaa aggactttca aaacaaaagg
    23101 tgcttcaatc cctaaagaat gtacagcagt caacgtcatc aaggaaatgg taatcgcggc
    23161 accgcccata ttgatcgtag cacctaacgg gatcgatatg gaataagact cttttgtcaa
    23221 tttcaatctt tcagccaaca tcatgtttat cgggatattt gcggcagaac ttcgggtaaa
    23281 gaaagcggga agtgcgcttt ctttcaaaca aaagaaaatc aatggatagg gattttcttt
    23341 aataaacaaa aacgtgagga atgggtacac aactaatgcg acaaacagca tcgtaccgat
    23401 caaaacaagt aaaagctgca gatactctgc caatccagcg attccagtcg tagcaattga
    23461 ttgataaacc aatcctaaaa tccctatcgg agcaaatgca ataatcactt gtacgacttg
    23521 tgacaacgct atagatatct gatccaccaa ctcttttgtc gattctgaac gtgctcgaaa
    23581 agctagtccc aacaatacag cccagaataa gacgccaagg taattcgcat caataagaga
    23641 ttgaacagga ttttgtgtga cattcgtcaa catagtagat aaaacacttt ccaaactttg
    23701 cggtgctttt tcagatactg tttcttgtaa agtaatagga acagtaaaaa ttgaacttac
    23761 aataactgct acaagtgcag aaagcaatgt tcccactaaa tataagatca aaataggctt
    23821 cacgaatgtc ttattcccaa ttttatgctt ggcaattgac gccatcgtca agaaaaaaac
    23881 cagaataggt gcgattgcct tcaagcaact gacaaacaac gttcccaata ttgaaataaa
    23941 tgtccatgtg gggaaaaaga taccaaacaa tattccgact aaaataccaa caacaatacg
    24001 ttgaactaac gacatatcca aaaacttctc taacaaatga ccaccctttc acaattttaa
    24061 caatcattgt atcattttat gcaaagaaac taaaggtata attttctaaa aattctaaca
    24121 atacaaacaa ccatttcttg ctaattttgt actttttctg tctacacgtt gttttttgag
    24181 atattctaag ttaggatgtt attaatgggc aacttatcag gaaacgtttt cttatgtttt
    24241 tgtccaattt ttttgtgagg tgtttattct tgaaaaaaat aaagaaggag agttatggct
    24301 tcctgacaat ctttgtcgct tgtataatga tggttttaca gcgtatattg atttcagcag
    24361 tcactattcc tacatatgca acaaaccctg gtcgattcta catctataca tttttacaaa
    24421 cttccgttgt tattggtgca aatttgatcc cactttacat gggttaccaa tatcaaaaaa
    24481 tcgcccgttt aaaagtttta cattatttaa gtcgttttgc ctttatattc ctaatagcca
    24541 cactagtttg caatatcttt ttttacgtac aagctggcag cttgaatatt cgtgattatt
    24601 ggcttatcct aacacctatc agtcagaatt actttactta tgctgtttct tgtactttgc
    24661 ttttttgtag tattccttat acagttggat ttttaaataa attatcgaaa acaacaatta
    24721 aatatctact attattttta acaattggat tagttggctg ctctactcta tttaataaag
    24781 acccgtttgc attacagaat ggacagagta ttttatggat tttttattta tttttaatgg
    24841 gttatggttt gaaagaatgg aactggaaag aaaaggtccg ctataagctt cctcaagtat
    24901 ttatcagctt gattgtttta ttcggtttga ttattttgat gacacaaatt tcactcatca
    24961 tcagaggaaa tacagataca gcgctgaggt tttctaaacc atattctctc ttcgcaatgt
    25021 attatacttt ctcttcgttt ttatttttag aaatgctatc tgataaatta ggtataaaag
    25081 gaagaggaaa ttttcttgct gtatttttga ttgtcactca agttatatgt aatttccctt
    25141 tagtttctta tcgcgtatct acatttatga aacgtgaatt tccaaactca gggaaggcat
    25201 ggctgataaa tatcgggcag ttttttattt gatatttatt agcagtgtcc ctcctagtta
    25261 tctttttatt ttatatacaa aaaatccgtt tcattcatcg tttcatcgaa tacttcgctt
    25321 ttgattcgat tgaacaatta actcagcgtt ggaaaaaaag aattcattgg ctttttgttc
    25381 ataaaagtta ttttttagtt gcagcatttt tttactgctt taccttcgtg cagattttcg
    25441 ttttagaacc aaaagaaggt tggaaagaaa ctatccaggt tgccttaaaa atatttactc
    25501 agcgacaagc acccatgatc ctgacaatca tcattattat gggatttttc ttcttactat
    25561 tgttgatttc caatcgtttt tggtacgcct tgactgctac cttgatcatt aacttactgc
    25621 tcactatttc gacagtaatc aaaatggaaa tgcgggaaga gcctgtcttt ccttcagatt
    25681 taaaaatgct gaccggattg tcagaactat tatcgatggt tagcccagtc ttattaatcg
    25741 tgggcggatt gatccttctc ctattattga ttactagtat tattgtacaa cgtagactac
    25801 aaaaacagta ttccttgaag attcattgga aacgtcgatt tatcagtatc gctgtattat
    25861 taggatgttt ttcgggtgtc tttttcatta atcacaaaaa ttcgccttct ttcttgctgt
    25921 ttaatttatt taaagtaaac aagactttct ttaaccagca aggcgccgta aaagataacg
    25981 gaccagtcat tcaattccta aacaatatcg acattaaaat catggaagaa cctgctggct
    26041 attcgaaaga aaaaattaat ggaataatga aaaagtacaa cacaaaggca gatgaaatca
    26101 accaaacaag aagtgactgg ttaaacaatc agacgatcgt tctcaattta agtgaaagct
    26161 tcagtgatcc tgcacgtgtc cctaatctga cagtacctac agaccctatt ccttacatca
    26221 aaagcattat aacagaagga acaggaggat tgatgttatc cgtcggttat ggtggcggaa
    26281 cagctaacat ggaatgggaa ggactgacag gcttgagtat cagtaacctg tctgcttcgt
    26341 tagttacacc ttatacccag ctagtggagc gtcagaaaat aagtccaaat attaccgatc
    26401 tattcgatga aaaaatagcc atccatcctt ttacagctgc tctttatcgc agaaaacaag
    26461 tgtttgataa atttggtttt gacaggttct attatttaga cagtccggat aaattaactt
    26521 atacggataa aatagaacaa agcccacgca tatcggatga ttcagcatac aaagaaacct
    26581 tgaaagcttt acaaagcaat acggaaacgt cacaattcat ccaattatcc actatgcaaa
    26641 accatatgcc ttatgatgat tactatagtg aattgaacta cactgctgaa ggaactgctg
    26701 tcattcctaa tagaagaaaa gaattacaaa cttatatgca aggccttcac tataccgata
    26761 cagcagtaaa aaaattcatc caagaaatcg acaaaatcga taaaccgatt acctttgttt
    26821 tttatggtga ccatttaccc gcaatctata gtgggaatga ttcgaaaaaa tatggattag
    26881 aacagcacca gacagattat ttcatttata acaacaagta tagtcgtgaa caagcagcta
    26941 cgttaaataa agaagttgtt gctcctaata actttggtgc tatggctctt gagcatgcga
    27001 atatcaaagt aactccttac tatgcgttgt tgacagaagt atctaatcat ctacctgcat
    27061 taacaattga tcctacagaa agcttgtcta atcgttttaa tggaaaacag gtctttgtta
    27121 cagaggatag tcaaattatc caagaaaaaa atttaactga gaaacaaaaa gaaattctag
    27181 aagattatcg tctgatacaa tatgatcttg ttgcaggtaa ccagtacagt gcaacatggg
    27241 cgacacaaaa aataaaagac taacaaataa
//
LOCUS       DAJSBL010000033        25501 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.033, whole genome shotgun sequence.
ACCESSION   DAJSBL010000033 DAJSBL010000000
VERSION     DAJSBL010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25501
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.033"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            133..3660
                     /locus_tag="ONT85_001711"
     CDS             133..3660
                     /locus_tag="ONT85_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HCR2378685.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3657..7379
                     /gene="addA"
                     /locus_tag="ONT85_001712"
     CDS             3657..7379
                     /gene="addA"
                     /locus_tag="ONT85_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HCR2378686.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7448..7789
                     /locus_tag="ONT85_001713"
     CDS             7448..7789
                     /locus_tag="ONT85_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR2378687.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8098..9141
                     /gene="pheS"
                     /locus_tag="ONT85_001714"
     CDS             8098..9141
                     /gene="pheS"
                     /locus_tag="ONT85_001714"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HCR2378688.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9146..11566
                     /locus_tag="ONT85_001715"
     CDS             9146..11566
                     /locus_tag="ONT85_001715"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HCR2378689.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="ONT85_001716"
     CDS             complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="ONT85_001716"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HCR2378690.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            13000..13737
                     /locus_tag="ONT85_001717"
     CDS             13000..13737
                     /locus_tag="ONT85_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HCR2378691.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13751..14566
                     /locus_tag="ONT85_001718"
     CDS             13751..14566
                     /locus_tag="ONT85_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HCR2378692.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14579..15226
                     /locus_tag="ONT85_001719"
     CDS             14579..15226
                     /locus_tag="ONT85_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HCR2378693.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15242..15898
                     /locus_tag="ONT85_001720"
     CDS             15242..15898
                     /locus_tag="ONT85_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HCR2378694.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            16060..16881
                     /gene="racE"
                     /locus_tag="ONT85_001721"
     CDS             16060..16881
                     /gene="racE"
                     /locus_tag="ONT85_001721"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HCR2378695.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16884..18239
                     /gene="rph"
                     /locus_tag="ONT85_001722"
     CDS             16884..18239
                     /gene="rph"
                     /locus_tag="ONT85_001722"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HCR2378696.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18240..18758
                     /locus_tag="ONT85_001723"
     CDS             18240..18758
                     /locus_tag="ONT85_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HCR2378697.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18865..19365
                     /locus_tag="ONT85_001724"
     CDS             18865..19365
                     /locus_tag="ONT85_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HCR2378698.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
     gene            complement(19645..20565)
                     /locus_tag="ONT85_001725"
     CDS             complement(19645..20565)
                     /locus_tag="ONT85_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HCR2378699.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
     gene            20730..21743
                     /locus_tag="ONT85_001726"
     CDS             20730..21743
                     /locus_tag="ONT85_001726"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HCR2378700.1"
                     /translation="MQIDWNNLGFSYIKTPWRFIAKWKDGQWEEGELTEDNYLTIHEG
                     SPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLMPMVPEKMFVDAVK
                     QVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVFCTPVGAYFKGGLK
                     PTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCVYLDPATHTKIEEV
                     GAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIEGDIFIDDLDQFAE
                     AGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGIQFGDIEAPEGWIE
                     VVE"
     gene            complement(21853..22608)
                     /gene="fabI"
                     /locus_tag="ONT85_001727"
     CDS             complement(21853..22608)
                     /gene="fabI"
                     /locus_tag="ONT85_001727"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HCR2378701.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            22828..23268
                     /gene="fabZ"
                     /locus_tag="ONT85_001728"
     CDS             22828..23268
                     /gene="fabZ"
                     /locus_tag="ONT85_001728"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HCR2378702.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            complement(23383..24144)
                     /locus_tag="ONT85_001729"
     CDS             complement(23383..24144)
                     /locus_tag="ONT85_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCR2378703.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            24333..25427
                     /locus_tag="ONT85_001730"
     CDS             24333..25427
                     /locus_tag="ONT85_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HCR2378704.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
BASE COUNT         8448 a         4424 c         5383 g         7246 t
ORIGIN      
        1 tttttgcgta tacttaaaaa agtagaaata tcagaacctt aaaaggatat aatttgaaga
       61 atatagaaca aatgttcagt attttttgat ataataaagg agcaccgtat catgtgttga
      121 aggaggaaaa gcatgtcttt acaatttatt cttggaaacg ggaaaaaaga tcaccgtcaa
      181 gtcttgatcg acgaagcatc cgcttggctc gaccaggatg caagtaacca agtatttttc
      241 cttgttccca actacagcaa attcgaacag gaacaagaaa ttttagcgga gatgcgaaga
      301 agaagcggaa aaaaaacttt cagcacaaca aacgttcaag tatttagttt ttatcgtctg
      361 gcttggtatt tgctgcaaca gacaactctt cttacaggaa atgaattatc agaatcagga
      421 tctgcaatga tcttgcgaca gattttagaa aaaaatgagg aagaattaac aatctttcgc
      481 ggagaaatca ataaaagagg gtttattcgt caattacaag aactctacag tgaattgcgt
      541 acaggaaata ttcaaccaga agacctgatg cttttatttg gtgattcacc taatgcagaa
      601 gaccagcaac ttaaaatgaa agacttgaag ctgatttttt cagcttatga cagcgaattg
      661 actcaacgtg cactaacaaa tgaagatgca ctatctatac tagcaaatta tatgcagaca
      721 caagatttca acaacgtcaa atttattgtc agtggttttt ctcgaatcaa tggtcaagag
      781 tatcaattac tgcaggcaat gatggaaaag gggcaattgg ttgtcgattt attgctggat
      841 cgtccttata cagcggatct tccagaagta cttacgctct ttcatgagac tggaaagctt
      901 tatttccgtc tttttcaaag tgcaagagaa cggaaagtcc ctatattagt ggaccgattt
      961 gcaccagagc agactcgtcc gacagagctt caactgctgc aaacaatgtg ggaagagaca
     1021 tctagccaaa agaaagcggc agtacacaaa atagggggac aagagcatct ccatttgtgg
     1081 gaagctgaaa atccaactga agaagtccga caattagctg ttgaaatcag acgactagtc
     1141 agtgaagaaa actaccgtta tcgtgatatc caagtgctga caaaaaatat gtctttatat
     1201 ggaaatatcc ttcggccgat ctttcaagaa atggcaattc ctttttatat cgatgaagaa
     1261 caaatgatgg aaaatcatcc gctgatcgaa tggatcaaca gtctgttcgc tcttgaccgc
     1321 tattcttatc ggctaagtga tattatgcgt tttttcaaaa ctgaattatt tctgtcgacg
     1381 gaagcagaaa ccgaacttga tgaatggaaa caagagcgag atgaattcag gaaaaaactg
     1441 gacatcactg aaaatgtagc acttgcttac aattatcaag gaacttattg gactcgtgaa
     1501 aaagactggc agtttgtggc gtatgatttt gaagcagaac agattgaaga cgtacagtat
     1561 ttggaagaac agtcaaatac gattagacga ttatttcaga aagctgtacc agtatttttg
     1621 aaaaaaataa agaaaagcaa aacaggacaa gaggccgcaa atgtttttta tcaatttttg
     1681 atccaaagtg gcgtacagca acaattattg ttttggcgta atcaggaagt agacagagga
     1741 aacttagaag cggcaagaaa ccatgagcag acatggggag cgctgattga tctgttggat
     1801 gaatatgtag taatttatgg tgaatcggct tttgactggt cactgttcca agaaatcatt
     1861 acaagtggtc tagaaaatct aagttatggt aaaatcccta ccgcaatcga ccaagtgcga
     1921 atcaatcgtt tggaattagt tcgaccaaat caagctgccg tcacttttgc attagggttg
     1981 aatgatcaag tgtttccaga ccgaaaagag acaaaaggat tgttgtccag tgaagaacga
     2041 gaagtattaa atcaggcctt gccagatgga cagtttttat tcgacccatc aaaggaaagt
     2101 atctcttttg agcctttcca agcctatctt gtttttcttt ccggaacaga tcgattatat
     2161 cttagttatg cacaaagcta tgacacagat ggttcgctga agatgtctcc ttatttgcgt
     2221 agaatcacag aatatttatc tgttcccgtc gagagaaaag agcatttgac gatcgaaagc
     2281 gaccctaatt gttatgtagg gacttacaga agcggaatca atacagtcaa taggatttat
     2341 cgattagctt tagatgaaaa aaaacctgta ccaaagcact ggcaaaagct caaagaatta
     2401 gtactgaatt cgtcatggcg aaattttgct ttacagattt ttgaaagtca ggaacattta
     2461 aatgttcctg tttcgatgcc agaaaaaatg gcggaagagc tttatggaaa agagatttat
     2521 acttcaattt cacgtatgga gaatttttat aattgtgaat acaaatactt catgcaattt
     2581 ggtttgaaat tgaaagaacg aactgtttat ggtctaactc cagcagcaac tggtgatttt
     2641 tatcatgaat cattggatcg tttcttcaag ctactgtttt caaaccaatt atccttagtg
     2701 aatatgtccg atgcgcagcg tagagacttt actgaaaaag tgctgcagga agtattcgga
     2761 gaacttcggt ttgagatatt ggatagttct gcaagaatga actatatccg ctaccagtta
     2821 ggacagacga ttcaaaaagt gacatgggct ttgcaaaaac agagcaaaaa tacaggcatg
     2881 catcctcttc agacagagat tctatttggt cagattgctg gagcaaaagg tatcacaggt
     2941 ctggaattac cgttgagtaa tggtggaaaa ctccatgttc gagggaaaat cgatcgaatc
     3001 gatgtagctt ctgaacaaga agatacttgg ctaagcgtcg ttgattacaa atctagcgga
     3061 cgttcattcg atgtcacaga agcttattat ggcatggcta tgcaattatt gacgtatctg
     3121 gacgtagcat tgatggatgc ggtacgtttg actggaaagg cggctgttaa accagcagga
     3181 tcatattatt tacatgttca taatcctgta ttgacggaga cagatgatgt agagaagaat
     3241 acattgaaaa aatttagtta tgatggcgtt tttgtcgatg atcccgctct tttagaagta
     3301 ctagatcatt cactggaagc aaaagaacaa tcactgatct atccagtgaa aaaaaatgcg
     3361 aaagatgaat accaaaaagg tagtttcagt aaagacaaat ttttcacgga agaagaactg
     3421 caactattca tggcgcataa tcgggaaaat atgcggttag ctggagatgc gattacttcc
     3481 ggagaaatca aactaaatcc agcttataaa gataaacagc ggattgcctg cgaattttgt
     3541 ccattcagaa gcatctgtac gtttgatgtg atgctgaaag aaaataatta ccaccgtctg
     3601 gagaaaatag ataaggaaga agcactgcgt agaatattga aaaggagtga agaagagtga
     3661 atcaaattcc tttaaaaccg caaaatgaac gattcacaga tgatcagtgg caagcgattt
     3721 ttgatcaagg agataatcta ttagtctctg cgtctgccgg atcaggtaaa actacagtgc
     3781 ttgttcgaag agtgattgaa aaactaaaaa tgggttttga tatcgatgaa ttattgattg
     3841 tgacatttac tgaagctgcc gcaagagaaa tgaaagaaag aattcaagag gcattacaag
     3901 aatccgtcaa tagcgaaagt gaccctgtac gtagacagca ttttactaag cagttagttt
     3961 tattgcccac agcgaatatc tcaaccttac atgctttttg cctgacagtt attcgacgtt
     4021 attattactt aattgatatt gatccagtct ttcgcatgtt aacagatgaa acagaaacga
     4081 tcttgatgaa ggaggatgtg tgggacgagc tgagagaagc actttatgca gaaaatgatg
     4141 aacgattttt ccaattaaca atgaattttt ctaatgatcg ttcagatgac ggcttgacga
     4201 atctggtgtt ttctctttat gagtttgccc gtgccaaccc tgacccccaa aaatggctag
     4261 aacagttaag cgataattat cgtttgcctg aggggctagc gaaatctcga ttatatcaaa
     4321 aacaaatccg tcctttagtt ttggcagata tataccaatg tgtacagtta tatgaacaga
     4381 tgactcagtt agcacaaggt gaaggattag aaaaaatgca tgagcaagta gctggagaac
     4441 aacagcagat caagaatatc tatgaagcgt tctcgcaaga tcggttagaa gaagcttatg
     4501 ctggtttgga acaactaact ttttcaactt ttaagagcag tcggaaagca gaattgaaag
     4561 aaatttccaa tgaagtcaag ggcatgaggg ataaagcaaa aaaactgatt cagcagataa
     4621 gtaaaagtta ttttcctgtt tccccaagcc aaatggaaga attaacggac aaagcactac
     4681 cgttagtcga agaaatgaca aaagtcacgc aaagcttcat ggacggtttc agtatgcgta
     4741 aacgggaaaa aggtgtgctg gatttcaatg atctggaaca tttagcgttg cagattctca
     4801 cagaaaaaac gaaagatgta tggctgccaa gtgaagcttc aaaacattat cgaaaaaaat
     4861 tcaaagaagt aatggtagat gaatatcaag acgtgaacca attacaagaa gccattttat
     4921 actggctaag agaacccgat gatacaaaag gaaatatgtt catggttggg gatgtcaaac
     4981 aatccatcta ttctttccgt ttagcagatc ctagtttatt tatcgggaaa tacgagaatt
     5041 tctcaaagaa agaaggcgga cgtcgaatcg ttttggctga gaacttccgc tctagaaaag
     5101 aagtcctttc ttttacgaat cttatttttg aacaactaat ggatccagca gtgggtcaaa
     5161 tcaattatga cgaagcagcc aaactgatcc aaggattttc agactttcca gagaatgaac
     5221 agtttgagcc ggaaatcatg atctatgaaa aagaacagga agaatcagag attgagattc
     5281 cgacggatga tatactggaa gacaaaacag aaggcgaact ttttatgaca ggactaaaaa
     5341 ttcgtcaatt aattgattcc tcctttatga tttatgataa aaaatcaaaa aaaagtcgtc
     5401 cgatagagta caaagacatt gttctcttga cacctacaaa gaagaacaac ttaacgattt
     5461 tagagatttt caaaacattg gatattccat tagagatgaa tgatgctcaa aactattttc
     5521 aagcgacaga aattcgcacg atgatctcgc tgctacaatt aatcgataat ccctatcaag
     5581 atattccgct tgcggctgta ttgcgctcac cgatcgtggg tctgattgaa ccagaattag
     5641 ccagcattcg attagctaac cgtgcacata cttactatga tgctgtgctt gcttatcaag
     5701 catcaaacga agatgaatta gcagctaagc tggaacattt tggcaagcaa ctagagcatt
     5761 ggcgagaact tgctcgccgt tcttcgatca ctgacttgtt atgggatatc tattatgaga
     5821 cgggatatct agagtatgtg gtcggtctgc cagcaggggc acagagacaa gccaatcttt
     5881 atgcattggt tgatcgtgcc aaagcttacg agcaaagttc tttcagagga ttataccagt
     5941 tcgtgcgttt cattgaaaaa atgcaagaaa aagataaaga cttagcagaa ccagtcattt
     6001 caatagaaga taatgctgtt cgcgttatga cgatccatgc aagtaaaggc ttggaattcc
     6061 cagttgtatt tttgttagat atgacaaagg agttcaactt acaagacttg cgcaatcggt
     6121 atgcttttga agaaaaactt ggagcaggaa ttcgctatat ggatcctgaa acacgagtgt
     6181 tatatgatac gttaccgttt caagccatca aattggcaaa gcaaaacaaa ttgttgtcgg
     6241 aagaaatgcg taaattgtat gtaggattga ctcgggcaga acaaaagctt ttcattgtcg
     6301 gttcctataa aaataaagag caaatgatcc aaacgtggtc tgaagcagct gatcatgagg
     6361 agttagtttt tgatcctgcg ctacgtttga agggcagaag cagcttgatg aattggattg
     6421 gttacggatt gatccgtcat cctgaaatgc agaaatactt agaggaagaa atttcgactt
     6481 ctcttcttca acattcaaat gcacagtttt ctatttcatg gatgaatcag caatcgatca
     6541 ttgaacaaag acagttgctt gctgaaaaag aactggttaa cttagatcag cagatgaaag
     6601 aagatgaaac tttattagca gattcgttac aaaaacgtct ggcatatgaa tatccttatc
     6661 aagcttcttc acaaaccaca agttatcaat ctgtatctga aatcaaacga ttattcgaag
     6721 atccagatga tacacaagaa tcacgtttga ctctggaaag cagtcagaac aaagcagcat
     6781 cccgtcaatt tcgttatacg caagaacaac tagcagaacc aaaatttttg caaaaagatc
     6841 ggcaagtgtc agcagctgct gttggaactg ctacgcatgc tttgttacaa ttactgccat
     6901 tagagatgcc aactacagag agtatccatc aaaaattaca ggaattggta aggaaacggt
     6961 tagtagacga aaaagtcgcg aaaaaagtag atgtttcttc tattatatgg ttctttcaaa
     7021 cagaacttgg tcaacagctg attgccaaca aagaaaacgt aaaacgagaa caaccttttt
     7081 ccatgctgct tcctgctgac gaagtatttc aagactatcc aaatcaagaa gatgaactat
     7141 taatccacgg aatcgtcgat ggatacctag aagaaaaaga tcatctcaat atctatgatt
     7201 ttaaaacaga ttttatttta catccggatg atccagcaga aatagatgca atcgtccaaa
     7261 aatatcaagg acagctaaga ctttaccaac aagcaatgtc agaagctttg aataagccag
     7321 tagaaaatgt tttcctaatt ttgcttcgag tgaaacaaat tataaacatt aacaaataat
     7381 tataattgag caaacttgac ttatgttttg taaggattat gctatactct gggaagaggt
     7441 gagtatgatg gagctaacac agacagaatt cgttttatgc cctaaatttg aaaaaacatt
     7501 ctctatttta ggaaaaaaat ggaacggatt gattatcgat gtactactcg aaaacggtcc
     7561 gcaacgcttt gttgagcttg cggcaaaaat cccgatggtc agtgatcgag tattagtcga
     7621 acgcttgaag gaattagaaa aagaaggaat agtcactcga aactttgcct gtagcgaaaa
     7681 gagccgttca gaatatctct tgactgaaaa aggagcagac ttgcaaaaag cgatgcgtca
     7741 aatacaagtt tggagcgaaa aatggatcac agaggaagaa tgcggttgat tttgtaaaaa
     7801 aaatcttgta aactatagtc catacgaata agtaaagaac gatgacgaaa aagagtagtt
     7861 tggctaaacc agttcaggga gatctgtcgc agactgaaag cagatcctgt gtttgaaaaa
     7921 tgaagttcac tttccgagtt cgtctgataa aatcagaccg gcaattgtcg ttaacgaagt
     7981 aagagtgcgg tgtaagtcat ttatccgcaa attaggatgg taacgcgaag acctcgttcc
     8041 ttaacaggaa tgaggtcttt ttgttttgta tttggaattt aaaggaggaa caagcacatg
     8101 tctctacaag cacaattaga gagcttgaaa gaagcaacgc tcgtcaaaat cacagcggca
     8161 caagatttaa atgaattaaa tcaaatacgt gttgaaacgt taggaaaaaa aggaccgatc
     8221 acagaagtat tacgtgggat gaaagatcta agtccagaag agcgaccaaa agtcggaagt
     8281 ttcgcaaatg agatccgaga tcttttgaca gaagagatcg agcgaaaaaa acaagtatta
     8341 gaagaagaag cgttaaatgc agctttggca aaagaaacaa tagacgtgac actacctggt
     8401 agaaaagtaa aacatggtac acgtcatatc gtgacacaaa tcatggaaga aattgaagat
     8461 atttttgtgg gtatgggcta tcagatcgta gaaggatatg aagtagaatc tgatcattac
     8521 aatttcgaac ggatgaatct gccaaaagat catccagcaa gggacatgca agatactttc
     8581 tatatttcag acgagatatt gattcggaca catacttcac cagtccaagc acggacaatg
     8641 gaaaaacatg atttctctaa gggtgctcta cgaatgatct cgccagggaa agtcttccgc
     8701 agagatacag atgatgcgac ccacagtcat cagttccatc aaattgaagg gctagttgtt
     8761 gataaaaaca tcacaatggg cgaccttaaa ggaacattag aagtcgtaat gaaaaaaatg
     8821 tttggggaag atcgtgaaat tcgtttgcgt ccaagttatt tcccatttac ggaaccatca
     8881 gtagaagtag atgtcagctg tttcaaatgt ggtggtgccg gttgtaacgt atgtaaatac
     8941 actggatgga tcgaaatttt aggagctggc atggtacacc cgaatgtgtt gaagatgtca
     9001 ggaatcgatc cagaagaata ttcaggcttt gctttcggcc ttggaccaga ccgcgtagct
     9061 atgttgcggt atggagtgaa tgatatccgt aatttctatc aaaatgactt acgtttctta
     9121 agtcagttca agggggagta agaaaatgtt agtttcatat aaatggctca atcgttatgt
     9181 agatttatcg aatgtaacac caaaagaatt agcggataag atgtctgtga caggaatcga
     9241 agtagaggga gtaacggttc cagaagaagg cttgaaaaaa atcgttgtcg gtgaagtcaa
     9301 ggaatgcgta ccacatccag attctgatca cctttctatt tgtcaagtcg atattggcga
     9361 agacgaactg tctcaaatcg tttgtggagc gcctaatgtg aaggttggaa tcaaagtgat
     9421 tgttgcactt cctaactcac gtatcgctgg taatgtaaaa atcaaaaaag gaaaaatgcg
     9481 tggccaagta tcaaatggca tgatttgctc attacaagaa attggctatt cagatagtgt
     9541 tataccgaaa gaatacgctg aaggtatcta ttatatgcca caagaagctg taaatggcga
     9601 tccagttttt ccttatttgg atatggatga ttcgattatt gaattatcta tcacaccgaa
     9661 tcgtgcggat gcattgtcaa tgcgtggcgt agcttatgaa gttggggcaa tttatcgtca
     9721 gactcctaca ttcgatgacg aaaagctggt agaatcctca cgtcctgcta gcgataaaat
     9781 tcaagtggaa gttgaagata aagaagcagt tcctgcttat caaatcagaa tcattgaagg
     9841 agtaagaatc cagccaagtc ctcaatggct gcaaaatctt ttgatgaacg aaggcattcg
     9901 tccaatcaac aatgtagtag atgtgacgaa ctatattttg cttttattcg gacaaccact
     9961 acatgcattt gactacgaca aattgaaatc aaaagagatc tttgtccgcc gtgcaaaaga
    10021 aaatgaaaca ttagtaacac tagatggaga atctcgggaa ttgacaactg acaatctcgt
    10081 cattactaac ggcaaagaac cagttgcttt agctggtgtc atgggagggt tagactcaga
    10141 gatcacgaat gaaacaacaa cagttgcact agaatctgct ttatttgatc caatttcgat
    10201 tcgtcgtaca gcaaaacaat ttaatttgcg tagtgaatcc tctgcacgtt ttgaaaaagg
    10261 aatcaaccat gcgactgttg gagaagccag tgaggttgct gcagcaatga tcgtttctct
    10321 tgcgggtggg gaagttctac aaggcgcggt caaaggctca gagatcaaag cagaagacgt
    10381 atcagtgaag atttcacttg aacgcatcaa tcaatattta ggaacatctc ttgtcgttaa
    10441 agaagtaaat gagatttttg aagcacttgg atttagctat gaagaaaagg agtatgttta
    10501 ttccgtagta gtaccaccaa gacgctggga tatccaaatc gaagcagata ttatcgaaga
    10561 agttgctcgt atttatggat acgaccagtt gccatctaca cttccaagtg gagaaacggt
    10621 tgctggaagc ttgtcaaaag aacaagaaac tactcgtaaa attcgtacaa tctttgaagg
    10681 aagcggattg agcgaagcaa tcagttatgc attgacaaca gaagaaaaat caagacagtt
    10741 tgttataaat gaaagtaata ttacacgttt ggactggcca atgagcgaag aacgttctgt
    10801 attacgaatg aatttgatca gcggactatt agatgatatc agttataaca ctgcccgaaa
    10861 aaatacagag attgctttat atgaaattgg tcgggtgttc ttccaagaaa atgatccacg
    10921 tacacatctg ccgcaagaag taaaacatgc agcaatcgct atttctggta tttgggaaga
    10981 aaaagattgg cagacgaaag ctcaaaatgt agactttttc accatcaagg gtcttttaga
    11041 aaacttattt gaacaactag gcatcactga agatgtccat tatcaaacag tgacagaaat
    11101 gaaggaaatg catcctggaa gaactgctgc gatttacctt ggcgaaaaat ttattggttt
    11161 tgtcggtcaa gtgcatccaa cgattacaaa aagttatgat attccagaaa catatgttgc
    11221 tgagtttgat ttatctgcta tagtggaagc tgctcaaaaa gggatcgttt tcgaagcagt
    11281 gacaaaattc ccagcagtca gccgggacat cgctttgctt gtaaaagaaa ctgtgacaaa
    11341 tcaggaatta acagaagtta tccgttctgc tgccggaaga tttttaacag atatccaatt
    11401 atttgatgtc tatcaaggag aaaacattga aaaaggacat aaatccatgg cgtatagcct
    11461 gacattcgtc aacccagaag caacattgac agatgaagaa atcaataaag caatggaaaa
    11521 agtcgaaaaa gcgttggtag aaaacctaga ggcaagtatt cgctaagaag gaaaatatag
    11581 taagtaacga tcaagtagct tttgaagacc atacatttga aaaaaagatg tgacacaact
    11641 tcgtcacatc tctttttttg attttaaaaa aggaatagca cagagacgag ataaaaaaac
    11701 aggcctttac agcctgcttt tttagagtaa atgaattaat ggaagtaatt gattcccatt
    11761 gctgatttta cttcagaaag tgtttgtgca gctactttag cagcttcttc actaccttta
    11821 cgtaagattt ccataacagc tgaaggatct ttttccaatt cttctcggcg cgcacggatc
    11881 ggtgcaaatt cagcttccaa tacatcaatg aggtatcgtt tgatctttac atcaccaagt
    11941 ccgccgcggc ggtaatgagc tttcatttct tcaattgctt ctttatctgt tccgaataca
    12001 tccaagtagg tgaagaccat attcccttct acttgtccag gatcttgtac atggatgtgg
    12061 tttggatcag tatacatact catgactttt ttctgtaaga catcggcagg atcggagata
    12121 taaataccgt ttcctaacga tttactcatt ttgccatttc catcgattcc aggtaatcta
    12181 cccattcctt ttggtgggaa tacgcctttt ggttccacca agacttcacc atatgtatga
    12241 ttgaagcttt gtacgatttc ctgtgtctgc tctagcattg gtttttgatc ctcaccaaca
    12301 ggtacaagat tcgctttgaa tgcagtgata tctgctgctt gagaaacagg atagataaag
    12361 aatcctgtag gcacgctttc accgaatttt ttttgttcga tttctgtttt gacagtcgga
    12421 ttgcgccgaa cgcgtcctac gctgactaag ttcaaataat acatggttaa ttcagctaat
    12481 tccggaattt gtgattggat aaataaagta gattttgcag gatctaatcc cacggctaaa
    12541 tagtctaaag caacttgcaa gacatttgat gaaaccttct caggattttt agcattatct
    12601 gtcagtgctt gcatgtcagc aatcatgaca aacagttggt tgtcttcatc tgcctgcata
    12661 gccactcggg tttttagaga accgacgtaa tgccctaaat gcagcttccc agttggtcga
    12721 tctcccgtta aaataatatt tttcaaaaaa gacacctctt tacgatttgt aagtctgagc
    12781 acttaatatc ttaccgtttt tcctgaaaag aaacaacagt tcctaaggaa aaaacaagga
    12841 aagaggataa aataactaat agattagaaa tggaagaaat atttatggga gaataaacgt
    12901 tctaagaaac tttacaaatt actaaactat cagatataat gtattttatt atacatcttt
    12961 aaaagaaatc ttactaaata ataatgggag tgtaattcta tggcaatgat taaatttgat
    13021 cacgtggata aatattatgg caagttccac gcattaaaaa atatcaatct cgaattcgag
    13081 aaaggagaag ttgtcgttgt cattggtcct tctggttcag gaaagagcac gatgcttcgc
    13141 tgtatcaatg gattagagac gatctcttca ggtaagctgc tgattaatga tactgacttg
    13201 catgataaaa agacaaaact gacagaagtc cgaaaaaaca tcggcatggt tttccaacat
    13261 ttcaatttgt acccaaataa aactgttctg gaaaacatca aattagcacc aatcaaagtg
    13321 ttgaagcaag accaggcaac tgcagtcaaa aacgcggaga aatttttgaa aactgtcaat
    13381 atgttagata agaaagattc ttatccttcc atgttatccg ggggacaaca gcaacgggtg
    13441 gcaatcgcca gaggtttagc gatgaatcct gagatgctgc tgtttgatga acctacttct
    13501 gctttggatc cggaaatgat tggtgatgta ttagacgtta tgaaaaaatt ggctcatgac
    13561 ggtatgtcaa tgattgtcgt gacacatgaa atgggctttg ccaaagaagt ggcggatcgt
    13621 gttatcttca tggctgatgg agaagtattg gaagacagca gaaatgtgag agactttttc
    13681 gaaaatccaa atgaagtccg agcacagcaa tttatcagta aagttatcaa tcattgatag
    13741 gaggaaaaag atgcgcaggt taaaaaaaat acgcctgatt ttccttttag gcattgcttt
    13801 tcttgcacta gccggatgta aaggaaatgg gttggcaaat gaagatattt tagagcgaag
    13861 caaatctacc aatgaaatca tctggggtgt caagtatgat actcgattat ttggaatgat
    13921 ggatattgag agcagaactg tacaaggatt tgatgtagat attgcaaaag caatcactaa
    13981 aaagatcctt ggtgacaatg gaaaaacaga atttgttgaa gtcacttcta aaactcggat
    14041 tccattattg aaaaacggaa atatcgatgc aattattgca acgatgacga tcacagatga
    14101 acgaaaaaaa caggttgact tttcagatgt ctatttcgat gctggccaag ctttgttggt
    14161 aaagaaagga agccaaatca aaagtgtaga tgatttgaat gcttctacaa cggttctagc
    14221 agtcaaaggt tctacatcgg ctgcgaatat ccgacagcat gcaccagatg caaaaatttt
    14281 agaattagaa aactatgcag aagcatttac tgctttacaa tctggtcagg gagacgcgat
    14341 gactacggat aatgcgattt tgttagggat tgcagatgaa aatccagagt atgagttagt
    14401 tggtggaacg tttactaatg agccttacgg gattgcgatc aacaaagggc aagaaaactt
    14461 cttgaaagct gttaaccaag cgctagagga aatgcatgca gatggcacat acgataaaat
    14521 ctatcaaaaa tggtttccaa atgaaactga aggaaaagta gagtaagggg gagaggcaat
    14581 gagcgaattg atacaaactt atggaccaat ctttgtcgac gggttcaaga tcacattgtt
    14641 ttcgagtgtc ttagcacttg tgtttagttt gattatcgga acgttaatgg caatcttcca
    14701 attatcaaag aataaagtag tgagcgcctt agcaaaggca tatgtagaat tttttagaaa
    14761 cattccatta ttaatcatcg tgatgttttt ttatgttgtc gtaccgattt attggtttaa
    14821 tctagatgga ttccaagcag gtacgattgg attaacgatc tatacatctg cttttattgc
    14881 tgaaacagta cgttcaggga ttcaaaccgt accaaaaggt caaacagaag caggactatc
    14941 ttctggtttt acctattcag aaatcatgcg ttatattgtt ttaccgcaag cattcaaaat
    15001 cgttatcccg ccattaggca accaatttat caacctcgta aaaaactcat ctattttagc
    15061 aatcgtagct ggtttagatt tgatgtatca aggagacttg atcgctagtg aaacattcaa
    15121 taccttcgat acgtatatca tcgttgggtt attttactta gttatcacgc taccgttatc
    15181 ttacttaatg gtttatcttg agaaaaaatg ggcggttcgt tcatagaagg aggggaaata
    15241 catggatttt agcggcgctt tttcatggat gaatatccgt tttttattgg aaggattaaa
    15301 ggttactgtt gaagtttcat tgttttcgat catttttagt ttcttgatcg gtggtctgac
    15361 aggtgtgatt cgttttgcga atatcccttt cttttccaaa atccttggtg tagtgattga
    15421 tattatacgg aatcttccat tattattgat cattttcttc acttactttg ccttacctca
    15481 aatcggcatt cagatgaaca tcttctggtc cgctgtcgca gcattaacga tttttgagtc
    15541 agctatgttg tcggaaattt tccgagctgg actcaatgct gttccaaaag gacagatgga
    15601 agcaggatta tcaacgggac tgacttatgt agaatcgatg cgaacgatcg tgttaccgca
    15661 agcatttaaa tcaatgatcc cggcaattgt gagccaattg atttcactga tcaaagatac
    15721 ttcattagca gtaatcattt ctttacctga actttctcat caggcacgta ttatttatgg
    15781 acagaacacc aattatgtgc ttccgatgtt catcatcatg acacttatgt acttcgtggt
    15841 atgttacgcg ctttctctgc tttccggtta cttggaaaag agacggtaca gttattaata
    15901 gatgattctt ttagcggttg tggcaaacta aagtcacaac cgcttttcct ctttccattc
    15961 gaaaaaaaga gatttcgatt gttttaagca agtaagagca cttgaaaatt cacgctaaaa
    16021 agtacacaat agggaggata gaatttattg aaaaaaagga tgatacgatt gacagataat
    16081 cgccctatcg gatttattga ttcaggtgtc ggcggcttga ctgtagtaaa agaagccctg
    16141 aaacaattac cgaatgaaaa tattttattt gtaggagaca cagcacgctg cccatatggc
    16201 cctagacccg cggaacaggt aatccagtat acttgggaaa tgacggatta tctggtggag
    16261 caaggaatca agatgctggt gatcgcctgc aataccgcaa ctgcggtggc tttagaagaa
    16321 atcaaagctg ctctttctat tccagtcatc ggtgtgatcc ttcccggtac tagagcggca
    16381 gtaaaaaaaa cacaaaataa acaagttggc attatcggta cgattggtac ggtaaaaagt
    16441 caagcttatg aaaaagcact gaaagagaaa gtaccagaat tgactgtgac aagtcttgct
    16501 tgtcccaaat ttgtttcagt tgtcgaaagt aatgaatacc attcatcggt ggcgaaaaaa
    16561 attgtggcag aaacattagc tcctttaacc actaaaaaaa tcgatacatt gattttggga
    16621 tgcacccatt atccattatt acgccccatc attcaaaatg taatgggaga aaatgttcaa
    16681 ctgatcgatt ctggagcaga aacagtaggt gaagtatcta tgctgttaga ttatttcaat
    16741 ttgagcaatt caccgcaaaa tggtcggaca ttatgccagt tttatacaac tggctctgcc
    16801 aaacttttcg aggaaatagc tgaagactgg cttggaatcg gacacttaaa tgtagaacat
    16861 atcgaattgg gaggaaaata aggatgcgtc atgacgggag aaaagcccaa gagcttcgca
    16921 aactggagct tcaaacaaat gttttcaaat atccagaagg atcggtagtg atccgttttg
    16981 gtgatacaac agttatttgt tctgcaacaa tagaaaattc tgttccccct tttttgagag
    17041 aaacaggaac aggatgggtg acagcagaat atagtatgct gcctcgagca acgagtacga
    17101 gaaatcgtag agaaagcagc aaagggaaac tttctggtag aacaatggaa atccagcgtc
    17161 ttattggtcg ttccttgcgt gcagtagtag acttggaaaa attaggagaa cgtagtatca
    17221 tcgtagactg cgatgtgatc caagcggatg gcggtactag aacagcaagt atcactgggg
    17281 catttgtggc attgaaatta gccattgaaa aacttttaag agaaaaagaa ttgtcagaag
    17341 atccaataaa agaacatctt gctgcagtta gcgtaggaat cttaccagat gggacttgcg
    17401 tcacagattt ggattaccaa gaagattccg cagcacttgt agacatgaat cttgtaatga
    17461 cagaatctgg aaaattcgtg gagattcaag gaactggaga agaagcaacg tttgatggtg
    17521 aacagttgaa tgaaatgctc ttttttggaa aaaacgcgat tgaagatttg atcaaagagc
    17581 aaaaacatgc tttgctaaca gagtttgctc aaaatgatga gcggatagaa gaaacaaaaa
    17641 caatcatcat tgcgacaaga aatccaggaa aagcagaaga gtttcgaaat atgttcaaag
    17701 aagcgggata tcatgtaaaa acattacttg actatcctga attgccagat gtggaagaaa
    17761 ctggcagcac atttgaagaa aatgcccgat taaaagcaga aacgattgca caattgcttg
    17821 atcagccagt cttagcagat gattcaggtt tgaaagtgga tgctttaggc ggtatgccag
    17881 gaatctattc tgcacgtttt gcaggggaac aaaaaagtga tgcggggaat aacgccaaat
    17941 tattatatga attgacagat gtgccggatg aaaaaagaac agcacaattc cattgtacgt
    18001 tagtattcgc ggcacccaaa aaagacagcc ttgtagtaga agcggaatgg cccggtcgag
    18061 tagcccgtat accatctggc gaaaatggct ttgggtatga tcctttattt atcccagaag
    18121 gtaagaaaca aacagcagca gaattatcga gcgaagaaaa aaacaagatc agtcatcggg
    18181 ctcaagcaat gaaaaaattg agtgccgagt ggaaacaatg gctggaagga gagagataga
    18241 tgcggtattt agtagtaagt gataatcatg gagacagaga tatcctcgtt gacttagtag
    18301 atcgttatcg ttctgaagtg gatatctttt tccattgtgg tgattcggaa ttagaagctt
    18361 ctgacccatt atgggataca ttccaagtgg ttcaaggtaa ttgtgattat ggaccaggat
    18421 tcgaacagaa aaaagtgatc caaactggtc aggatacagt attcatgact cacggtcatt
    18481 tatcgaatgt ccgatttggt ttgacacagt tagcaatcga agctcgagct gcaactgccg
    18541 atatggtgtt ttttggtcat acccatcaaa taggctgtga gatggatcag aaaatcctgt
    18601 ttttgaatcc tggtagcatt tctcagccta gaggcccgat ccaaatccca tcatatgctc
    18661 ttatcgaaag tttagatgac caaatttctg ttcaatacta taaccgtgcc catcaggcag
    18721 ttgaagagtt ggccttttct ttttcgagag agcgctgaat aaaaaaactc taatttttaa
    18781 taaaaataag ctttatttta tgaattttgc atccggtttc ggtaaaatag tagtgttgaa
    18841 tgaaatagaa atggaggcca tcagatgatt ggacctatag taagagaaat gctgctggaa
    18901 aatcaggaaa cgtttttgat tcctgcagaa aatgtggcaa atgttatgta tcatcatcca
    18961 ttgtcccatg gtttacttgt gttatcgaag gtcggttatt ccaaaattcc tgtattagga
    19021 aaagatgatc actttgtagg actagtaagt ttatcagatg tggtcaataa aatgattgac
    19081 ttgcaaacaa tcagcatgga cccgttagaa ggactaacgg ttgctgatgt gacagaaaca
    19141 gatgttccga caattgatga aaattgggaa ctagaagaag tactccattt gttagtcgat
    19201 tcgccttttt taccggttgt cacagaagat cggattttca aaggaatcat tacaagaaaa
    19261 gagctgctta aagccgtaaa cttcatggtg catgaattag aacgtcgtaa tatattgtct
    19321 ccgaaaatcg aagaattaaa agaacaaatc gatatcgtga gctaaagaac gagaaactaa
    19381 aagataatca aaaaataagt agaaaaaaca gcgtaccagt tttgacttta tcttgaaaaa
    19441 gaggctggga caaaagcgtt taactccaag aaataagaag gaattcacga aaattgcttt
    19501 tcaaattttt gtgaatttca gcttatttcc gaaggagttg cttctgcttc cgccgtttat
    19561 acgtgtttag agcgtgggac aaaagtgttc tttacttttg tcccacgctc tttttagtga
    19621 gattcgttgc atcgtttgtc caaattattt tccgacagca attggactgt tcggaatcgt
    19681 gaaaccagaa gctgtcaatt ctttgacata ggagctcaag aactcttctt ttaaggcata
    19741 ttgttttccg ttcaatgcgt agcagaccgt acgaatcgca aaattgctgt ttccaagatc
    19801 cactaatcca aagatagaag gttcagtttg gagctcttct tggtattttt cagctaatgt
    19861 acgatttact cgatcaatga tctcatagat tttgtcatat ccttcttcag gaacaatccg
    19921 aatatctaaa agtacttgca tatttgcacg agagagattg ctgatcgtgg tgatgtttcg
    19981 attcgggatg aaatggacag taccatccgt tgctttcagc tgaagggtac ggataccaac
    20041 agatacgacg gttccttcaa tcgttaagtt tgctagcttg atgtagtccc caacatccat
    20101 ctgttgttcc atgataataa aaaatccggt aataacatca ctcatgaacc cttgggcacc
    20161 aagaccgatt gccacacctg caattccggc accagccaaa agtgatccga tcggcacacc
    20221 gatagcagat aaaattgcat agataaaaaa gaaaccaagg gtataatgaa aaatagtatt
    20281 taaaagacta tgtaaagtct ttaagcggct ctcgtttaga atggcttttt tagaatactg
    20341 attaaaagac ttatcaatca gatattttcc cacacggtga agaatccaaa aaaggacgat
    20401 caaaaaaatc agatataaac ttttttggat aaaggcagcc aaaatggcgt cccaatcaat
    20461 cccgtcccaa aaccgctgaa acgcatttac tttgctgaca gcttgatctg tcaatgtctc
    20521 gccagaagag tctgtcgtac cggcagttgc taataatgaa aacataaaat cctcttttct
    20581 aaaatttgat tctttctgta ttgtaccaag aagttttcaa aaaaggaaat agcaaaaata
    20641 actataatga aggaaaaaag ctgaaaataa aatgacaaat aatatggata atgataaaat
    20701 gtggagaaaa taacgaaggt ggtaagtata tgcaaatcga ttggaacaat ctaggttttt
    20761 cttatatcaa aactccttgg cgttttattg ccaaatggaa agacggacaa tgggaagaag
    20821 gagaattgac agaagataat tacctgacga ttcatgaagg ctctccagcg cttcattatg
    20881 gtcagcaatg ttttgaaggt ctaaaagctt atcgtcgaaa agacggcaag gtcaatctct
    20941 ttcgacctga acaaaatagt cgacggatga atcaaagtgc tagacgattg ctgatgccaa
    21001 tggtccctga aaagatgttc gttgatgctg tcaagcaagt ggtaaaagca aatgaagatt
    21061 ttgtgccgcc ctatggaaca ggcgctacat tatacttgcg ccctttactg attggggtcg
    21121 gtgaaaacat tggtgtccat ccagctccag aatatttatt tgtcgtcttc tgtacgccag
    21181 taggagcgta tttcaaaggt ggactaaaac caacgaattt tattgtatct gattacgatc
    21241 gtgctgctcc taaggggact ggtgcagcaa aagtcggcgg aaattacgca gcgagtttac
    21301 ttccaggaac agaagcaaaa gaactggatt actcagattg tgtttatctt gatccagcca
    21361 cacatacgaa aatcgaagaa gtaggtgcag caaatttctt tggaattaca aaagacggaa
    21421 aattcatcac tccgcaatcg ccctctatct taccaagtat cacgaaatat tccttgttgc
    21481 agttagctga agaacgacta ggactgcaag caattgaagg agatattttc attgatgatc
    21541 tagatcaatt tgcagaagct ggtgcatgtg gtacagcggc tgttatttct ccaatcggtg
    21601 ggatttatca caataacaat cttcatgtat tttatagtga aacggaagta ggaccagtaa
    21661 caaaacggtt gtatgatgaa ttgacaggta ttcaatttgg agacatagaa gcaccagaag
    21721 gctggattga agtcgtggaa taatttcaaa gtaatgagtt gaaaaaaacg taaaaagaaa
    21781 aaatcaggaa gagactttaa aaagtttttt cctgattttt atatgtttta ctttaaagaa
    21841 tgcaacaaaa gtttatgaaa gatggacacc tttgtcaacg tagataatat caccgatgat
    21901 tccgctggaa agtgggctga ttaagaaagc acaagcatta ccgacttctt cgatcgtcac
    21961 accttgctga tcaggcgtac gagattcaga taactggatc agtttttgat agtctttaac
    22021 acctgtgaca gctaatgttt tgattgcccc agctgagatt ccattgaccc gaattccttt
    22081 aggcgagaac tcagctgcca aataacggat agcagcttca agagaagctt tcgcaatacc
    22141 catcatgtta taattcggga ttgcgcgttc tgaacctaga taagtcatgc taacgatccc
    22201 gctgtttggt gcaagaaacg gttgtgcgta ttttgataca gcaattaaag agtagctgct
    22261 gatatcttga gctaattgat agccgtctct ggaaatatca gacacatttc cggacaattc
    22321 ttctttgttg gcgtaagcaa ctgcatggac aaggccgtgg atctccccag catattcacc
    22381 gatggcattc attgcttttt cgatgctttc gtcgctagaa acatcacatt ctacagtgaa
    22441 tgcttcttcg ccgaccaatt tgactaatga ttttttcatt cgttcatttt ggtaagtata
    22501 gataatttct gccccttgtt tcaacattgc ttcagcacaa ccccaagcaa tgcttttttt
    22561 gttagcgacg cccataatga cgattttttt tccttctaga aatgacataa tgactctcct
    22621 ttgtttctga taaaaatttg atagtcgtaa aaaaatagag aataatagct aaaataactt
    22681 cttgatttct taattattag tcaaaatgtt ttgattgtca aactattttt tcttaaatta
    22741 aaaaaagatt gctaaatatt taggaatatg atactttgat agtcaaagta atgaaaatgg
    22801 caattgcctg aaagaaggag tttaattatg tcttacgtat tatctgctac tgaagtaatg
    22861 gaattgattc ctaatcgtta tcctatttgt tatattgatt atgtagatac tttagaacca
    22921 ggaaaaaaga tcgttgcgac aaaaaatgta acaatcaatg aagatttttt ccaaggtcac
    22981 tttcctgaca atccagtgat gccgggtgcg ttgattctag aaacactggc acaagcaggt
    23041 tcgatactta tcctaaaatc agaagagttt ctaggaaaaa gagcttatat cggtgggatc
    23101 aataaggcca aattccgtca aaaagttgtt ccaggagatg tgctgaaatg tcattttgaa
    23161 atcaccaaaa tgaagggatc agtcggtaca gcgttatctg cagcatatgt agatgataaa
    23221 aaagtctgtg agtgcgagtt cacgttcatc gtcaaccaaa atgcttgact ataaaacctg
    23281 atatagcaaa acaaaaggaa gcttacttgc tttttatgta aaaagaagac gcctgaatcc
    23341 tagcatacgt tgtcatctat gcaggattca gacgttattt ttttattttt gttttgcttc
    23401 ttgcagtata ttgattttat cgatttttac ttcttctttt ggtttgtctg aactatctgt
    23461 ttcgatattt gcaatagcat ccacgacatc cattccttca atcacttggc cgaagacagt
    23521 ataatggcca tctagtgaag gataaccgcc gtttttataa gcgtcgatga ttgcttgagg
    23581 atagtcgtct tttaaaagac catcatgcat atcatctgta ttttgaacga taaagaactg
    23641 gctgccgtta ctgttaggat cttgtgagcg agccatagat aacgcacctc ttatattata
    23701 aaggtggttg ttgatctctg tttcaaatcc ttctccccaa atgctttccc ctccagtgcc
    23761 atcgccttta ggatcgccac cttggatcat gaaatctttg atgacgcgat ggaaagtcag
    23821 tccgttgtag taaccgtctt ttgcatgtgt cataaagttt tcaaccgctt ttggtgcgtg
    23881 ttcgggaaat aatttgattt tgattgtgcc tttattcgtc accatttcga ccagatcttc
    23941 attttcggcg acatctttcg tcaattgtgg taattccaat gcgttcaaat cgacgctact
    24001 ggcagtcgtg ctttccgcag tagatgtact ttttgaactt tctgtagacg aactagaaga
    24061 agtcgtgttg tttgcagaac aaccagcagc aaacagcaat gctgctgcta aactaatact
    24121 taaaataact ttatttttct tcataactgt tcctttccaa tttactattt tgttatcttt
    24181 aatgataaca aaatagaggg aaaataccta cttaaattat tggaatttat catttatttt
    24241 aaaagaaaaa gaaaaggtgt agaccattta ctttgtgttt gctataatgt aagtggatac
    24301 aaaaacgaaa agatgcagga ggaaatggta atatgggaaa attaggaatc tcgatttatc
    24361 cagaacggtc aacatttgaa aaggacaaag cgtacttgga tttagctcat aagtatgggt
    24421 ataaacgggt ttttaccagt ttgctgcaga tcaatgatga taaagaaaaa gttctttcag
    24481 aatttaaaga agtagttgat tacgcgaata gcctaggcat ggaagtaatg gttgatatca
    24541 atccagcgtt atttgaacag ttggagattt cttacgatga cttatctttt ttccataaga
    24601 tgggtgccta cggggtccgc ttagatatcg gattcacagg agcagaagaa gcaaaaatga
    24661 cgcgcaatcc ttttggtatc aaaatcgaga tcaatatgag ttcgggcacg aattacgttg
    24721 acaacatcat gagttattcg ccaaacacgg ataatttatt aggttctcat aatttttatc
    24781 ctcatcgata ctctggttta ggatacgaac actttgtttt ttgttcagaa aaattcagaa
    24841 aatataattt gaatacgatg gcattcgtca attcgcaatc tgctgagttt ggcccttggc
    24901 ctacgcaaga tggcttgtgc acgctggaag accaccgaga tcttgaaatt gcgacacaag
    24961 tgaagcattt aatccttaca ggattgattg atgatatttc tattggaaat gcgtacgcaa
    25021 gtgaagaaga attgaaagaa atggcagaag catttaacgc ggattatcca acattgaaag
    25081 tagatacgga agaaggtatt acagaaaacg aacgaatctg cttgtttgat aaccttcata
    25141 gttatcgagg ggatcgctca gaatatatcc ttcgttcaac gatgactcgt gtatactaca
    25201 aagataaaga tttcccgcct cataatacgc gagatatgca tcatggggat gtactgattg
    25261 acaatgaagg gtacggtcaa tacaaaggag aaacacaaat cgccttgaag gatatgaaaa
    25321 acgacggacg tgtcaatgtt gtaggaagaa tctccgatga cgaattattc ctccttgact
    25381 ttttgaaacc atggtcaagc tttaaattaa ttgaaaataa aaaatagata attagttatt
    25441 gcagaaataa tccgctagaa atagccagtt atttctgctt ttttattttg tttttttata
    25501 g
//
LOCUS       DAJSBL010000034        23831 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.034, whole genome shotgun sequence.
ACCESSION   DAJSBL010000034 DAJSBL010000000
VERSION     DAJSBL010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23831
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.034"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(55..789)
                     /locus_tag="ONT85_001731"
     CDS             complement(55..789)
                     /locus_tag="ONT85_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HCR2378705.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1116..1526
                     /locus_tag="ONT85_001732"
     CDS             1116..1526
                     /locus_tag="ONT85_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HCR2378706.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1519..2220
                     /locus_tag="ONT85_001733"
     CDS             1519..2220
                     /locus_tag="ONT85_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HCR2378707.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2220..3806
                     /locus_tag="ONT85_001734"
     CDS             2220..3806
                     /locus_tag="ONT85_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR2378708.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3825..4046
                     /locus_tag="ONT85_001735"
     CDS             3825..4046
                     /locus_tag="ONT85_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HCR2378709.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4043..4804
                     /locus_tag="ONT85_001736"
     CDS             4043..4804
                     /locus_tag="ONT85_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HCR2378710.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5184..5693
                     /locus_tag="ONT85_001737"
     CDS             5184..5693
                     /locus_tag="ONT85_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HCR2378711.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5768..6307)
                     /locus_tag="ONT85_001738"
     CDS             complement(5768..6307)
                     /locus_tag="ONT85_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HCR2378712.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6447..7058)
                     /gene="rpsD"
                     /locus_tag="ONT85_001739"
     CDS             complement(6447..7058)
                     /gene="rpsD"
                     /locus_tag="ONT85_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HCR2378713.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7320..8171
                     /locus_tag="ONT85_001740"
     CDS             7320..8171
                     /locus_tag="ONT85_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HCR2378714.1"
                     /translation="MRGYVQMEKSEGSLRRKSMNPVSPLFEKIDASIEKKANLFENSF
                     SRYAVRAVLACLFLTLGTAVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAEL
                     GTSNMLYMTVGVFRQRLALSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNF
                     MLESIAVKLAKPSATILVEGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLG
                     YEHVIANFPAFTLTYFASHGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQ
                     SKSSYVD"
     gene            8199..8834
                     /locus_tag="ONT85_001741"
     CDS             8199..8834
                     /locus_tag="ONT85_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HCR2378715.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8854..9627
                     /locus_tag="ONT85_001742"
     CDS             8854..9627
                     /locus_tag="ONT85_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCR2378716.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10035..10832
                     /locus_tag="ONT85_001743"
     CDS             10035..10832
                     /locus_tag="ONT85_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HCR2378717.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10810..11223
                     /locus_tag="ONT85_001744"
     CDS             10810..11223
                     /locus_tag="ONT85_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HCR2378718.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11207..13852
                     /gene="mutS"
                     /locus_tag="ONT85_001745"
     CDS             11207..13852
                     /gene="mutS"
                     /locus_tag="ONT85_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HCR2378719.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13870..15978
                     /gene="mutL"
                     /locus_tag="ONT85_001746"
     CDS             13870..15978
                     /gene="mutL"
                     /locus_tag="ONT85_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HCR2378720.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            16000..16560
                     /gene="maf"
                     /locus_tag="ONT85_001747"
     CDS             16000..16560
                     /gene="maf"
                     /locus_tag="ONT85_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HCR2378721.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(16645..18633)
                     /gene="treP"
                     /locus_tag="ONT85_001748"
     CDS             complement(16645..18633)
                     /gene="treP"
                     /locus_tag="ONT85_001748"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HCR2378722.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            18856..19569
                     /gene="treR"
                     /locus_tag="ONT85_001749"
     CDS             18856..19569
                     /gene="treR"
                     /locus_tag="ONT85_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HCR2378723.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            19646..20092
                     /gene="msrB"
                     /locus_tag="ONT85_001750"
     CDS             19646..20092
                     /gene="msrB"
                     /locus_tag="ONT85_001750"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HCR2378724.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            20262..20864
                     /gene="ruvA"
                     /locus_tag="ONT85_001751"
     CDS             20262..20864
                     /gene="ruvA"
                     /locus_tag="ONT85_001751"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HCR2378725.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAIEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            20877..21878
                     /gene="ruvB"
                     /locus_tag="ONT85_001752"
     CDS             20877..21878
                     /gene="ruvB"
                     /locus_tag="ONT85_001752"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HCR2378726.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            21907..22485
                     /locus_tag="ONT85_001753"
     CDS             21907..22485
                     /locus_tag="ONT85_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCR2378727.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            22537..23019
                     /locus_tag="ONT85_001754"
     CDS             22537..23019
                     /locus_tag="ONT85_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HCR2378728.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            23136..23510
                     /locus_tag="ONT85_001755"
     CDS             23136..23510
                     /locus_tag="ONT85_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2378729.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
BASE COUNT         7783 a         4285 c         5114 g         6649 t
ORIGIN      
        1 ataaaaaaac ttggtacgca atcgctgtac caagtttttt tatttagctt ttgattaagc
       61 cataaataat aatttatata agaatactgc tacgacacca gcacagatag gtgctacaac
      121 tggtacccat gcatatcccc attgagaaga acctttatgt ttcaatggaa gaagtgcgtg
      181 tacaagacgt ggagcaaagt cgcgggctgg gtttagtcca ggacctgtag gaccaccaag
      241 tgaagcaact aatgtccata caaggaaacc aagtgctaag tgagctgtac caagattatc
      301 ttggaagaat ggtgcttttg taatacctag ggcaccaaag aataaaacga atgaaccaaa
      361 gaattcattg acgaaaccat tgaatttaga accaacgctg ttgattgttg agaatgtacc
      421 taacacatgg tttacgtctt ctgtttggtc gtagtaaggt ttgtaacaag ctactacgat
      481 caattgtcca atcgctgcac caatcaattg tgcaatgata taaggaacaa cttcagccca
      541 agggaataga ccattagatg ctaaagcaat tgtaaatgct gggttgatgt ggttaccgct
      601 gattccacca aaaatcattg ctggcatcat aaccccgaat ccatacccga cagcaatcag
      661 catccagtca ctgccatatc cttttgtacc ttttaaatct acgtttgcta cagcaccgtt
      721 acctagaacg attagtaatg ctgtaccaat catttccgcc gcaagacggg tcattagtga
      781 gtaatccata aatacctcca aaagttataa aaatgcaaaa aaaaaagaaa catcaacacg
      841 aaaagcatcg gtgcagtgtt tcgtgtcgga aggatttctg cagtcttaac aaatgtgaat
      901 aaaaaaatga attccattac ttataagaaa ccagaccaac agtaaaactc caactctttt
      961 taaaagttct ccacaaatca ccaagcttaa ctataaatat accaaaaaaa aaacgttttt
     1021 tttctcaaaa attgtatctt agaaacagca tgttttgaaa ctttcagaaa aggtttgtta
     1081 gtaaaatgaa agctatacgt aaggagtgtt gtttgatgaa attttttcaa gagtttttca
     1141 ttattatttt cttttctgta ttaggagaag gcatacggat ttttgctcat ttacctattc
     1201 ctggaagtat cttaggtatt ctttttttat ttttagcatt tgaaatgcat cttgttgatc
     1261 ctgaaaaaat cggagcaact ggtgattttt tattgaataa tctagccatt ttatttgttc
     1321 ctgctggtgt aggtttattg gaatattttg atgatattgc ttcgatatgg cctgttttac
     1381 tgggagcagt cgtcgtgtgt tccatcgtca cgatggttgc agcgggaaaa acggcagaag
     1441 gtgtagaagc tttgctgggc tatgtccgta agaaaaaagc agaaagggta tctgtaaacg
     1501 aaatggagga gaatcaaatt gactgagttt tttgtgaatc cattatttgg cttaagtttg
     1561 actttaggag tgtacgtatt atctgacaaa tttttgcgga aatataaact gcctgttttg
     1621 aatcctctcg tattatcgat tgttgttatt atcttatttt tgcatttttc tgggatcagc
     1681 tataaagact ataatatcgg gggcaatttt ttgactatgc tgattactcc tgcgacagtg
     1741 gcacttgcca tccctcttta taaaaacttc cgtttactga aagagaattt ttatcctgta
     1801 atggctgcga ttttagcagg aatagtcgca aattgcttgg ttagtgtagg gttcggttac
     1861 ttcttctctc ttcataaaga gatggtgatc tcattactac ctaaatctgt aacgacggct
     1921 atttctgtcg atttatctca tacgatgggc ggggtgaatg ccgtgacact tgctattgtt
     1981 gtctctacag gtatttttgg ttcgctgatc gccactcata ttttccgcct attcaagatc
     2041 gaaagtccag ttgctcgcgg tgtggcgtta ggaagtactt ctcatgctat tggtacagca
     2101 aaagcaatcg agattggtga aatagaagga atcatttccg gacttgcgat atgcgtcaac
     2161 ggaatattga ccgttttatt gctaccgtta ttctttcagc cttttgctgg cttgttttga
     2221 tgcgaaaacc actattttat gctacaataa ccaaaacgtt cgagcataaa aaaggaggga
     2281 aagacgtgta tttgtcttcc gagtatctta aacaatttga taaagatcta tattataaga
     2341 ttctcctcct agaaagtttt gaggatcaag cttggcatac cgctgcacaa ttagcgcaag
     2401 ttgttcagct ggatgcccgc agcgtgtcga aatacctgaa cgagctgtct aaaaactatc
     2461 agcagtttag cggtaagaca caccctttgt ttacaaaaaa tcaccgttct ggttataact
     2521 tttatgatac gctcgattca attgaacatg agcgtttttt gatctatctc gtgcaaagta
     2581 cgctgaaatt ccagttgctg cacgacattt tttttgaaga attccataca atgtaccaat
     2641 ttgcgcaaaa gcattacatt agtgaatcta ctgctcatcg aaaaatcaat gaatggaaac
     2701 agcaactgca aacttacgga attcggctgc aacgaggtac atatatcgct caaggcgaag
     2761 aagaaatcat tcgactttac ttgcatatga cattctggca gttgtttcga gggaaaatct
     2821 ggccatttga aacgatttct caaatggacg taaagaatat ggcagaacat atcatggctt
     2881 tttttaatgt tcgtctgaac gagatcaaaa aaagacgatt ggaatatatg ctaggtgcct
     2941 tttttttaag aaaatcacag aagcattatg ttgtattgaa tgagaaaaaa agacggctga
     3001 tttctgacaa tctgttgttc cagcgatttt gccaggtcat ggagccggta tttcctaatt
     3061 attttcaagt agaagacgag ttaggcgcat tatttcttgt actgatgact cgtgaagaat
     3121 attatagtga tcctaagata cggaagaaaa tttttgattt tcatcaacaa gcaaaaacac
     3181 cgccctttac agccctttca gaagcaaaag ccgctctttc tctttatcag gaagagcagg
     3241 ggctgccagc agaaaatcta accttcgagg cagaaaacta tttattcagc agtcattttt
     3301 tcgcctatct ttttcctaat gcgaaagaaa cgattgatgg caatagtagt gattttatta
     3361 atcatttagt tattgaaaac aaggaattga aacaatggct cgtacatttt tttgaaagta
     3421 gacacaaaca tcccaatcat ctggcattta aaaatcatgc gtttttgatg gggcggtatc
     3481 tgactgtgtt taaaacatta ggagccttta ctccacagct tccaaagatc acgatccttt
     3541 taatgacaga tttcccatta tttgaagagc aactgttaga ggagggatta cggacatttt
     3601 tccgtaatga gtatcagctg atttttctgc caacagatta tcgggggaga gaagttgatc
     3661 ttctgatttc gacaagtaaa gtccaccgca aaccatgggc ggatcttgac tattttattg
     3721 tgactgaaga actaaaatta attgattaca tccaactatc tcaaaaattt gaaatgattc
     3781 aaaaacaaaa actattaaaa caataacaaa gaaggagaaa aaagatgaca tttgaagaaa
     3841 ttttaccaga attgaaaaaa ggtgcaaaga ttattcgtaa aggatggagt ggatttgaac
     3901 tttatgttgt tctcgtatcc gatgatgaat ttgatggctg tccggtgacg ccgtacttat
     3961 tgatcaaaac aagtgacgaa ggtttttcca gcttcgctcc tacagtttgt gatattttag
     4021 cagatgattg ggcgatcgta gaatgaaaaa ccagtatcca gatctagcta aacaggttgt
     4081 atttatcaca ggagctgctt ctggaattgg tgctgcgcaa gcccgagcgt ttcttgaaca
     4141 agatgctttt gtatttggat tagatcaaaa gttcggtaaa atggaagaaa tttctttgcg
     4201 atttcctgaa acttttgcct acgcactagg tgatgttcga caaatgaaag aactccaaca
     4261 agcggtatcg aaatgccaaa atcattttgg tgaagttacg attttactga atacagcggg
     4321 gatattggat gcctacaaac ccttgatgga aactgacgaa tcgctgtggg atctgattta
     4381 tgagacgaat gtgaaaagca tgtatcagct gacaaaaatc atccttcctg atatgatccg
     4441 ccaaaaaagc ggaacgatca tcaatatggc ttcgattgct ggcttgattg ctggtggagg
     4501 tgggatcgct tatacaagcg cgaaacatgc gattgtcgga tttaccaaac aactcgctct
     4561 ggattacgca ggacagggta tccgagtaaa aggaattgct ccaggagcca tccaaacacc
     4621 aatgaatgcg gctgattttg aagggaaagg tgagatggca gagtgggtgg caaatgaaac
     4681 accagtcaag cgatgggcac agccggaaga agtagctgag ctgacgttat ttcttgcctc
     4741 tcctcaagca agctacatac aaggaacgat catcccaatc gacggcggat ggttgttaaa
     4801 ataaccttgt gtttttttca agcttctgta tgataaaact aaagagagaa acaacggttg
     4861 atgataaaaa aagtcgatga ctgttcccga cattcgatgc cgaacagcca gttcaaaaaa
     4921 ttctttttaa taatcgttaa tattcgaaac taagcggctt ttcacgacct cttgatataa
     4981 acgatattca aaagcttgca actgcggtaa caagtccagc caaatgaaaa atataaggag
     5041 ctatttttat ttgaccattc ttattacttg ttgcagggca gctttttcac taccttctgc
     5101 gacacttcag ttggaatttt cttcccatcc tttggtcgcg gtcattacca agataaaatt
     5161 atcttgcaaa ggagaaatag aacatggaaa aaaataatac ccgttcagcc aaacgctgga
     5221 caacgacaga tgtgactcgg atggcgctag tcactggttt atatgtggca gtgacgctgg
     5281 ttttgtctgt ctttagcttt ggagtgattc agattcgtct ctcggagatg ttcaactatc
     5341 tgtccttgta taacaaaaga tacatcgtcg cggtaacagc gggagtagct ttggctaata
     5401 ttgcttctcc tcttggactg atagatgttg cagtaggaag cgtcagcacg ctgcttgtat
     5461 tgcttatcaa ctataaaatc actagtcgca tcaaaaacat gaaaatcaaa atggccgtga
     5521 cagcatgcgt atttgctttt tccatgttta cagtagctgg tcagctaacg attttatatc
     5581 aactaccttt tttcttgaat tggtgggtga ttgctttagg agaattggca tcaatgattg
     5641 tcggtgggat aattatttat tggatcggta aaaaagtgga tttgtcaaaa taaagatctg
     5701 tttaatttaa aatagggcag catggaagag actgaatgtt gagtcttctt ccatgctgct
     5761 tttttgatta atttgtatga agaaatcgat ttagccgttt tttcatcaag ataccgatat
     5821 aagctgctcc agttgccttg attgcttccg gcagtaaaaa cggcagtagt ccactggcaa
     5881 atgcagttgt aaaagtaaga tcaccactta ttttcagcca gatcgttccg cagatcaatg
     5941 cagcaagaga gcccattaga ttggcaaaga ttgcatataa gtaatgagca tccgttcttt
     6001 cgatcatcca accagttatc cacgtttgta agagaaatcc aatcaaatat ccgccagttg
     6061 gcccaaataa aacagctata cctccagtca tccctgaaaa tacaggaaga ccaatcaagc
     6121 caagtaaaag ataaactcca acagatatca atcctagttt tcttccgaga aaagttgctg
     6181 ttaatccaat agcaaatgtt tgccctgtca aaggaaccaa accaaaagga atggtcatct
     6241 gagaaaggac agaaataatc gcggcaaatt ccgcagcaag tatcaattct ttcaatgttc
     6301 ggttcataac gccctctttt cgttaactta atatttttat ttggttaacg aaattgtaaa
     6361 acaaagtgat tactttgtaa atagaaaata taaaaaaaga ccagctatcc aatagatagc
     6421 ggtctttttc aagaaagctt ttgaaattat aagctttggt tgtagtattc aacgacaaga
     6481 gcttcgtcga tttctggata tagttcgtca cgttctggta aacgagtcaa tgaaccttcc
     6541 aatttttcag cgtcaaagct tacgaatgct gggcgtccaa cagttgcttc tacagcttct
     6601 tttactgtta cgatgttttg tgatttttca cgaactgaga tcacttgacc cacttctacg
     6661 tggtatgaag ggatatcaac gcgtttgcca tcaactaaaa tgtgaccatg gttaactaat
     6721 tgacgtgctt gacgacgagt cgttgcaagt cctaaacggt aaacaacatt gtctaaacgt
     6781 tgttctagta agatcatgaa gttaacacca tgtttacctt ctttgatttt gctagctttt
     6841 acgaacaagt tacggaattg acgttcgttc attccgtaca tgtgacgaag tttttgtttt
     6901 tcagttaatt gcatgccgta ttctgattgt ttgccacggc tattaggtcc atgttgacct
     6961 ggtgcgtaag ggcgacgtgc taattctttt cctgtgcctg acaatgagat gcctaagcga
     7021 cgagaaattt tccatgatgg tcctgtataa cgagacatta aaatttcctc caatgaatat
     7081 aagtttttgg agtaaaataa tccgttgaaa aattcataat catatagttt gttcttcaat
     7141 cttcaccttt gcagccgcgg ttacacaatc gaacccgaga cggggcaaca aactagggat
     7201 gagttattat ttttctgctg cattatttta cacaaattac agtatatgat agatcatcca
     7261 cgaatgtcaa gataattagt acataaaaac gctttacaaa cctaggctgt ctttataaaa
     7321 tgagagggta tgtgcaaatg gaaaaaagcg aaggaagttt aaggagaaaa tcaatgaatc
     7381 cggtatcacc attatttgag aaaatcgatg cttcaattga aaagaaggcg aatctttttg
     7441 aaaacagctt ttcacgttac gctgttcgag cggttctcgc ctgtttgttt ttgactttag
     7501 gaacggctgt ggcatttgcg atcgcgatga agggggaaga tatcgcgcat ggtctaggaa
     7561 agattttata cgcatttatg ttcagctggg gtctggtcat gattctatac atgaacgccg
     7621 aattaggaac atcgaatatg ctttatatga ccgtgggtgt ttttcggcag agacttgctt
     7681 taagtaaggc agccaagatt ctatttgcct gtattttttt caatatgatt ggcggtatat
     7741 tatgtggatt tctaatttct atgactacgc catttcaaga tttaacgttg gacaacttta
     7801 tgttagaatc aatagcagta aaactagcaa aaccttcagc aacgattttg gtggaaggga
     7861 tgtttgcgaa tatcgttgtc aataccgctg tactgatcag catgcggatg aaagaggatg
     7921 tcggaaaagt gatcacagtc gtattcatca ttttcatttt tgctttttta ggatatgaac
     7981 acgtgatagc gaattttcct gcctttacgc ttacttactt tgcttctcac ggacagatgg
     8041 atggaatgac agtcagcaat gtgttacata atttgttttt tgctttggca ggaaattata
     8101 ttggtggcgg tttagtaatg ggactgggat atgcttggct gaatcagtca aaatcatcct
     8161 atgtggacta aaaagtcgtt cattaaaggg gaagtgccat ggaagtttta gtttttccaa
     8221 aaaatgagga aaaagcgtta caaatggaaa agtacatgaa aaatcttttt tcctttgctg
     8281 gagtacctaa gccggaacgc gctcttttac aacaaccgtt actggatgag agcaaaaatt
     8341 tatctatttc tgaagtttta gcttttgttc attattatta tgaacaaatc gaaagagagt
     8401 atcagtatgt agcgatcgat ttagtccaga aaaacatcaa acggcttaca tttgaagact
     8461 tagtcgtatt gttgccacta gttcagaaaa aagaatggtg ggacagcatc gactcttgga
     8521 gaaaagtata cagcacatgg tgtaaagcct acccagagca gcgtgaagaa gtctttcaat
     8581 tgtttgaaaa aaaggaagat ttttggctac gtcgaatagc aatcacgttg caattaggtt
     8641 ataaacagca aacagacaca ggactattga tccgagcaat agacgaagat cgtcaaacaa
     8701 aggaattctt tatccaaaaa gcaattggtt gggcattacg cgattatagt aagaccgatg
     8761 ccgattgggt aaaaagacag ctgacaacag atttgagtaa gttagctgtt cgagaggcaa
     8821 gtaagtattt ataagaaagc gagacgacaa gagatgagac gcaaattatt tgcttttgat
     8881 attgatggaa cgttgttaga ttcaaacaaa caagctttag atagcacaag agaagcattg
     8941 gaaaaattac gcaaagccgg tcatctggtc acgattgcta cagggcggag ccgatttcat
     9001 gcgcaagaga tcattcgcga tcttgatttt acgaactaca ttttatgtaa tggagcggcc
     9061 ggttttttag accatgaaca agtatataag aatttactag atcgcgacca gttggatgcg
     9121 ttcgttcaag aagcgcatca tgagcagatc gatacagctt ttgtcagctt agacaatatc
     9181 aagcgttctt cttccaatga tgtaacggtg atggaagaag cgatgcattc tttcggtgct
     9241 gtcttgcctg aattagatca gttttttgca gaggaacaag aaatatacca agcgttagca
     9301 tttttcgatc aaacatatga tgggaagttc tcttcatatg ataaaattcg atttgtccgc
     9361 tggcatgaga acagtgtaga tgtcgtacct aaaaatggtt ctaaagcagc gacaattctg
     9421 cacttagcag aacaagtagg catccctcat gaagacatca tcagctttgg cgatggacaa
     9481 aacgaccgtg aaatgttgcg catgtcagga attgggatag ctatggggaa tgctgtgcca
     9541 gaagtacaag cagaagctaa gatggtcact gatacaaacg atcaggatgg aatttggaag
     9601 gcattgaaag agttaagcgt gatttaaaag aggttgtgaa aaagctgttc tgcatcaagt
     9661 aataagccca gccaaacaaa aatagctcct cctatttttc gtttttctgg gcttattacc
     9721 gcagatgcaa gctttcgaac accgtttaag gaagaggtcg tgacagaagt ggtcagtttc
     9781 aagaaataag aagggattta cgaaaattgc ttctcaaatt tttgtaaaat tacggcttat
     9841 ttctgaagaa gtagatgaaa caacgtttat tcgggaaatg ggactgtgac aagatgtttg
     9901 tcatagtccc atttttttat tttatttttc aaaactaaga aataccagga aagagttcct
     9961 tttgtttgac cttttctttc ttattgaaaa caactataat atagagtgat gttaaaagag
    10021 aagaggaatt tagtgtgcgg gtattgttta ttggtgatgt agtaggttca atgggacgag
    10081 agatgattac tgaatatctt cctagattga agaagaagta ccgtccgcaa gtaacgattg
    10141 taaatggtga gaatgccgct tctggacgtg gaatcacaga aaaaatctat aaaaaatttt
    10201 tgcaagacgg cgtagatgtc gtgacgatgg ggaatcatac gtgggacaac cgcggaatct
    10261 ttgagtttgt aaatgaagct aaaaagatgg ttcgtccggc aaattttccg gaagggacgc
    10321 ctggacaagg gatggtcttt gtcaaagtga accaagtcga actggcagtg atcaacatgc
    10381 aggctcgttc ttttatggtg gacttggatg atccttttcg taaaatgaat gagctggtag
    10441 aagaagccag aaaacgtacg ccgatcattt ttgtagattt tcatggagaa acaacaagtg
    10501 aaaaacaagc aatgggctgg tttttagatg ggaaagtttc agcagtggtc ggtacacata
    10561 cacacgttca gacaaatgat gcgcgaattt tgccaagagg aacagcgtat ctgacagacg
    10621 ttgggatgac aggtccctat gatggtattt tgggtatgcg cagagaacct gtaatcgaaa
    10681 agtttctgac tgcattacca aaacgttttg aagttgtgga acaaggccga tcgattttaa
    10741 gcggctgtat cttggaattg gatgatacga caggtcatgc aaaagaaatc cagctgattc
    10801 agatcaacga tgatcgaccg ttcatggagt agaaagagag gcagtttttt gcagcaggag
    10861 ccggaaattc aaaaagaagt aaggaaactg acaaaacttt taagagagaa tgaaacgatc
    10921 attcgctaca aagaacttga agaaaagatc cagcaaaacc agtatttagc tgagttgaga
    10981 gaaaaaatca aacaagcgca aaaagatgct gttcattttg cccactatga caaaccagca
    11041 gcagaaaaag aagcaatcaa acaagcggat caattcatgc aagaatttga tcagcatccg
    11101 cttgttgtag cttatagaaa acagctgttg gaagcagatg atctgcttca tcatttaaca
    11161 actatgatac aagaagaaat caatggacaa atagaggagg aaaaacatgc ctcaaaaaac
    11221 taaaaatact cctatgatgg aacaatatct aagcatcaag gcccaatacc aagatgcttt
    11281 tttgttctat cgtttaggag acttttatga actattttac gaagatgcga tcaaagcgtc
    11341 acagcttttg gaattaacgt tgacgagcag aaatcgaaat gcagaagaac ctattcctat
    11401 gtgcggtgta cctcaccatg cagcacaagg atatatcgat gccttgattg aaaaaggcta
    11461 taaagtagcg atttgcgagc aggtagaaga tccaaagacg actaaaggga tggtcaagcg
    11521 cgaagtgatc cagctagtca ctcctggtac agtcatggac agcaaaggat tgtcggcaaa
    11581 agacaacaat tttttaacag ctgtagttag tcaaggaaat gaatttggct ttgcttatgc
    11641 cgatttaagt acgggagaat tgaagacggc tcggttgcat gatgaagaag ctgttttaaa
    11701 tgaagcctct gcattgcaga caaaagaact cgtattaggc agtgagatca ctgataccct
    11761 tcgtgaacag ctaagcgggc gtttagggct agtcttttct caacaaaatg agatggaaga
    11821 aaacgcggaa tttagctttt tgacaagcga actagtcgat ccgctggaaa aagaagcaac
    11881 cggcaaacta ttgacttatt tatcggtgac acaaaagcgg agtttagctc atatccaaaa
    11941 agcagtagaa taccaaccag atcatttttt gaaaatggat tattattcaa aattcaacct
    12001 agaattaagc caatctattc ggactgggaa aaagcacggc actcttctat ggttgttaga
    12061 tgaaacaaaa acagcaatgg gtggccgttt actaaaacaa tggctagacc gtccgcttat
    12121 ccaggcaaaa caaattaaag caagacaaga aatggtcgct tctttgctag acagctattt
    12181 tgaacgtatc gatttgcagt cagcattaac gaaagtctat gatctggaac gcctagcagg
    12241 tcgagtagct ttcggtaatg tcaatgggcg agacttgatc cagctgaaaa cttctttaga
    12301 acaagtgcca atgatcagag ggttgattga aggaatcgat caaggtcaat ggcaaagtct
    12361 gctttcagaa atgcagccga tggaccatct cgtccaattg atcgaccaag ctatccaaga
    12421 tgatccgccg ttacaaataa cggaaggaaa catcatcaaa gatggattca atgagcagct
    12481 cgatatgtac cgttctgcta tgagaaacgg aaagaaatgg ttggcagaac ttgaagcaaa
    12541 agagcgtcaa gaaacaggaa tcaagaactt aaaaatcgga tttaaccgcg ttttcggtta
    12601 ttatatagaa attacaaaag caaatcttgg aaatgcagaa ctggagaagt atgaacgaaa
    12661 acaaacttta gcaaatgcag agcgtttcat cacaccagaa ctaaaagaat tggagacaca
    12721 aatcctcgaa gcagaagaaa aatctgttga tttagaatat cagttgtttt tagctgtgag
    12781 agaagaagtc aaaaaggcta tccagccgct acaagtactt gcaaaagcaa tcagtgcagt
    12841 agatgtgcta caaagttttg caacgatcag cgaacgctat cagtatgttc ggccagaact
    12901 tgtcagtgac aagcatcagc ttctgatcaa agacggacga catcctgtag tagaaaaagt
    12961 attaggacat caggaatata tccctaacag cgtagaaatg gcagaagacg aaatgatctt
    13021 gctgattaca ggaccgaata tgtctggtaa aagtacgtat atgcgacagt tggcattaac
    13081 tgttctgatg gcgcaaatgg gctgtttcgt tccggcaaaa caagcagttt tacctatttt
    13141 tgatcggatt tttacccgaa ttggtgcaag tgacgactta attgctggac aaagtacttt
    13201 tatggtagag atgatggaag ccaatcaagc cctgcgttat gctacgccaa acagtttgat
    13261 tttgtttgac gaactagggc gaggaacagc aacctatgac ggaatggcgt tggcccaagc
    13321 aatcatcgaa tatatccatc gtcatgtaca agcaaaaaca ctcttttcta ctcattatca
    13381 tgaactgacg gtacttgaga aagaacttcc gcagcttaaa aatgtccatg ttggagccgt
    13441 tgaaaaagac ggagaagttg tcttcttgca taaaatgatg gatggacctg cggataaaag
    13501 ctacggcata catgtggcaa agatcgcagg attgccttcc aatctactag aacgagcagc
    13561 agatatcctg catacgctag aaaatggcga taacggacac catactgttc cagctgaagt
    13621 agaagagaaa actaaaacag ccaaacagga gcaaataaaa ccacctgcag aaaaagtaaa
    13681 tcaagaccat atacaagacc gtgtaaaaga agaaaaccaa cagctttctt tattcagtga
    13741 attatccgaa aatgaaacag aagtcattga atcactgaag caaataaatt tactagaaat
    13801 gacgccaatg gatgcactaa acacattata tgaattacaa aaacaactgt aggaagaagg
    13861 cggatatcga tggccaaaat ccaagaatta tctgaacgtt tagctaatca gatcgctgcg
    13921 ggagaagtag tggagcgacc agcttccgtt gtgaaagaat tagtggaaaa tgcaattgac
    13981 gccggcagta ctcagattga tatcttagtt gaagaagcgg gactgcggac gatccaagtg
    14041 attgataacg gagaaggcat tttggcagac gacgtagaga atgccttcaa gcggcatgcg
    14101 acaagtaaga tccataatcg cgacgatctt tttcgcatac gaacgctggg ctttcgaggg
    14161 gaagctttgc caagtattgc ctctgtttct gaaatgactc tggaaacggc gaatcaggaa
    14221 gaaaaacaag gcacctatct gtcgctgaaa ggcggagaag ttattgagca tcgaccagca
    14281 ccactgcgtc aaggaacgaa gattaccgtc agcaatctgt ttttcaatac gccagcccgc
    14341 ttgaaatacg tgaaaacact gcacacagaa ttagcgaaca tcggggacat cgtcaatcga
    14401 ctggcattga gtcatccatc gattgctttt cgtttagtac atgatggaaa caaaatgctg
    14461 gcaactgctg gaaatggcga tctgaaacaa acaattgcgg gaatctacgg attatctacc
    14521 gcaaaaaaaa tgctgaaaat cgaaggaaaa gatctagatt ttgagttatt tggctatgtt
    14581 tcattgcctg aggtgactcg tgccagtagg aattacttat cgacgatcat caatggacgt
    14641 tttatcaaga acttttcctt gaataaagca atcgttgcag gttatgggtc aaaattaatg
    14701 gtcggtcgtt tccctattgc tgtattggaa atcaaaatgg acccgctttt agtagatgta
    14761 aatgttcatc cgacgaagca ggaagtccgg ctgtcgaagg aaaaagaact aacagagcta
    14821 attagtaacg ccattcaaaa agcattgtct caagaaaatc tgattccaag tgcagcggat
    14881 aatctacggt ttaagaaaaa agtacctgat cagcctaaaa cagaacaaat cgaaatcgat
    14941 ttgtcctacg cttttgaaga agaaaaacct aaaaagccaa gtagtttgaa ttttgatcga
    15001 gagacaggta cctttttcgt agaagaacta aagaacacat ttgttgacag aattgttgat
    15061 aagtctgtgg aaaatgttga aaactattct ggtttttctg aagaaaacac cacttatcca
    15121 gaaaaaacag cgaaaacggt ggataagttt ggtgaaaatg ttgataactt tgtagaaaaa
    15181 acagtggaaa aatctgaaaa gaaagaattt cccattgtgg ataacatgga gaaaacaaat
    15241 gaagaacaag cagataaatc cgtagaagag gatgaaaaga tttacgagga agaattgaca
    15301 ctccatcctg aatttgattt cactcaagga aacgacaaaa cattagaaca agccatcaag
    15361 aaattagaaa cggaaaaacc agtacaacgt tttcctcagt tggaatattt tggacagatg
    15421 cacggaacgt atctttttgc ccaaagtagc gatgggttgt acatcattga tcagcatgct
    15481 gcccaagagc gcattaagta tgaatatttc cgggaaaaaa ttggggaagt atctgatgat
    15541 ctgcaagaat tgcttgttcc atttgtgtta gactatccaa acagtgatgc tttgaaatta
    15601 aaagagcaaa aacacttgct agaagatgca gggatttatt tagaagaatt tggtcaaaac
    15661 agctttattg tacgggccca tcctacttgg tatcccaatg gagaagaaga atcgattatt
    15721 cgggagatga tcgatatgct gctgacaact ggaagtgtga gtgtaaaaaa attccgtgaa
    15781 gcaacagcaa tcatgatgag ttgcaaacga tcgattaaag ccaaccatta tttgaatgaa
    15841 gcacaagcac gtgtgctgct gaaggatctg gcacgatgcg aaaatccttt caattgtccc
    15901 catggccgtc cagtattgat tcactttacc aattcggata tggaaaggat gttcaaacga
    15961 atccaagacc cgcattaagt caaagagaag agggaaaaca tgaagattgt attagcatcc
    16021 caatcaccaa gaagaaaaga gttattggct catatcgttt cagaattcga gattgaacct
    16081 gctgatatcg atgaaacgcc atttccagac gaacagcccg tcgattatgt tcgtagaatg
    16141 gctgaagaaa aagcacgaac tgtctgggaa caagatgaaa aagaagacca tttagtgatt
    16201 gccagtgaca cgactgttgt tctcaatcaa gagatcatgg gtaaaccaga agatctggct
    16261 gaggctgaat tcatgctgaa aaaactaagc ggggaaacac atactgtata tacagctgtt
    16321 gttttaaaaa caaaggaacg agaagaacgg atactagcag aagcacatgt cactttttat
    16381 cctttgacag acgaagagat caaacgctac ttggagacgg gggattatgc ggataaggca
    16441 ggggcatacg gcatccaaaa tgctgcgagt gtattcgtca aagaaatttc tggagattat
    16501 tacagtatcg taggatttcc gattggtgct gtcaatcagg cactgaaaac attcatttaa
    16561 acaaaaaagc ggactatctg attttcgatt gaaaatccat gatagcccgt tttttttatt
    16621 gaaaagatta cttatctact ctgactaaga ttttacttca gctttaccga ctgcttcgcc
    16681 cgggcgaaca agtcctgatt ggtcaagaga aagtcctgca actttttcat tattggtcaa
    16741 agcaacaata atgtcagttt tcttgccagc ttgttctatt tctggcaaat ccatttcagc
    16801 aatcagggtc tctggtgtga caaggtatcc ttctttcacg aatacattga aaggaacacc
    16861 ctttagttca acagtatcta atcccatgtg aacaagtact tccagcccct cattagaaag
    16921 aataccaatg gcatgttttg tttcaaagac actcgtcact ttaccgttta ctggtgcata
    16981 gactttgccg tttgacggtt cgacagcata accgtcaccc atcatttttt gtgagaaaac
    17041 tgggtcattt acttcagtta tttccttgat ggtccctgca gcgactgcga ataatgtttc
    17101 ttttgttgca actgccgtac cagttgctga aacaggttcg aaagactgtg gcgttgctgt
    17161 tgcgccattt gctgtttgaa gttgtacatc agcagcattt tcaggaacag caggatcaat
    17221 cttattcaaa attccctgac gtctaaagat gatcgtcaag ccaaatggaa tgatgacagc
    17281 aatgatcatt gcgatgatga aaggtaccca tgtttctgct tggatagcta gtattcctgg
    17341 caaaccgcct actccgatcg cgttagctcg aacacccatc aagttagcaa acatgccagc
    17401 taatccagag ccgaccattg cagcgacgaa aggatagacg tatttcaaat tgattccgaa
    17461 catagcaggt tcagttacgc ctaagtaaca agaaatcatt gctggaatcg agatttgttc
    17521 ttctttttta ttcccacgat gtaagaagat gatggctaag actgcagagc cttgggcgat
    17581 atttgataaa gcaatcatcg gccataaatt cgttgatccg aaatctgcaa tcagctgcat
    17641 atcgatggca ttggtcatat ggtggagacc agtaatcact aaaggagcat acaggaatcc
    17701 gaatacggca ctaaacaacc agctgataga agacgtcaat ccagtattta cgatatttga
    17761 gatccagctt ccgactgtcc agccgatagg acctaaaata acgtgtgcag ctaaaacagt
    17821 tggtaacaat gaaaacaatg gaacaaaaat catcgagatc gactgaggga tatatttgcg
    17881 gaagaagatt tctagatacg ctaacataaa ccctgccagc atggctggaa taacttgcgc
    17941 ttgataacca atcatctgga cttgagcaaa accaaaatcc caaaatggaa tatctgctgc
    18001 ggctgtggaa gctacactat aagcatttaa taattgcgga gaaactaacg taattcctaa
    18061 aacgattcct aatatttgtg tagtacccat cttttttgca atactccaag tgatacctac
    18121 tggaagaaaa tggaaaattg cttcacccgg taaccacaaa aaggcattga caccattcca
    18181 aaacttcgag tgttcaacga ttgttccacc aagactttca aactggatac cttcaagtac
    18241 atttctgaat ccaaggatca atccaccgac aatgattgca ggaatcagtg gtgtaaagat
    18301 ttctgctaaa acggcaatag cacgttgaac gggatgaagg ttttgttttg cagctgcttt
    18361 tccttgttct ttggaaaccc cttcaactcc agatactgca acgaagtcgt tataaaaagt
    18421 agaaacatcg tttcctatga tgacttggaa ctgtcctgca ttcgtgaaca tcccttttac
    18481 gctaggtata tcttcgatcg ctttttcgtc cgcttttcct ggatcattca ggacaaaacg
    18541 catacgagtc gcacaatgac taactgcagc aatattttct tttccgccga tatcatgtaa
    18601 cagcttctcg gcatctgctt gatattttgc catatcaata aacccctttc tcttaacttg
    18661 tctatgcaag ttgtttcttc atttactata ctttttttcg gagagaattg caagcgtttt
    18721 attaaaaagt acgagagaaa atgatatcgt tttaaaaagc ttaaaataag cttttcttaa
    18781 aaataggaac attgaaatac aaaaagagct gtagtatagt gaagaaatga tggaagaacg
    18841 agtgagggaa aaaacatgaa taagttcaat gaaatttttt tagatttgga acagaaaatt
    18901 ttagcgaaag aatatcctcc gcattcgctt ttacccagtg agaaccaatt gatcaaaatt
    18961 tatggtgttt cacgagaaac aattagaaaa gcgctgaatc ttttgactac ggaaggttat
    19021 attcaaaaaa aacaaggaaa aggttcgatc gtactggatc ggaatcgctt cgactttcct
    19081 atttctggat tgactagcta taaagagcta caggagaccc aacgtattcc aagtgaaacg
    19141 atcatccata cgttagaaga aacagaagtg acaaaagcat tgaacgagat cactggctgg
    19201 gagatcggtg cgcctgtttg gcatttgata cgcgaaagaa aaatcaatgg cgaagtagtt
    19261 atattagaca ccgattatct attaaaggaa atcgttccta ttttaactcc agcacaagca
    19321 caaggatcga tctatgaata ttttgaaaat gagctttctt tgacgattgc ttacgcgcaa
    19381 aaggagatca cagtagaaga agtaacggaa atcatgaaaa cagcgatgga tcttcacgat
    19441 gacacttatg cagtagtagt cagaagcctc gttcatctgg aagatacccg ctgtttcgag
    19501 tattctgagt cggttcaccg attagataaa ttccgtttcg ttgagtttgc ccgacgccga
    19561 aaagtgtaaa tagttcaaaa gaaagcaaat tgttggcatc tgtccacttt ggcgctatga
    19621 taggatcaca aagggggcat ttttaatgga taaaccaata agaccagaaa aagaagaact
    19681 gaaagaaaag ttatcaccca tggcttatgc agtcacacaa gaaaatgcaa cagaacgacc
    19741 atttagcggg aaatatgatg atttttacga aaaggggatc tatgtagata tcgtcagcgg
    19801 tgaaccgtta ttcagctctg cagaaaaata tgatgcaggt tgtggctggc catcattcag
    19861 caaaccaata acgaaaagac aagtgcgcga aaaagccgat ttttctcatg gcatgcatcg
    19921 aatagaagtt cgaagcaaag aagcagattc tcatctcgga catgtgttta ccgatggacc
    19981 agtagatcaa ggtggtctac gttactgcat caattctgcc tcactgaaat tcattccata
    20041 tgaaaagatg gatgaattag gttatgggga attcaaatca cttgtagaat aatggaaata
    20101 cacaaagagc ggtacaatac ggggatttac tcgatcgtcc gctctttttt tattcaagcg
    20161 attgctattt gaaagaaacg ctccctagac tccttttttc acaaggtgaa atgtgctaca
    20221 ataaagggaa cgtatgtgtg caaaagaaag aggactagaa tatgtatgaa tatctgacag
    20281 gtgtggttac ctttatcaac ccctattaca tagtagtaga aaccaatggt atcggctacc
    20341 agatcgcact aggaaatcct taccgctaca gtagcaaact aaatcaaaaa ataaaaattt
    20401 atgtccatca ggtaatacgt gaagatgcgc atcttctgta tggatttgat tcgctagaag
    20461 aaaagcaact gtttttgcga ttagtaagtg tttcaggaat cggtccgaaa agtgcccttg
    20521 caatcatggc aagcgaagac cactccggtt tgattcaagc agtagaatcc agtgatgtca
    20581 cttatttgac gaaatttcca ggagtaggga aaaagacagc acaacaaatg atcttggatc
    20641 taaaaggaaa attcggtgag ctgagtatcg atacaccttt tagtctattt gatgaagcaa
    20701 acacaaaaga cgctacagct ctatctgaag caatagaagc tctctcggct cttggttaca
    20761 gtgatcgaga aatcaaacgt gtagaaaaac aattaaaaga agtagataat cagacaacgg
    20821 atgagtatct acgccaagca ctcaagctaa tgatgaaaaa ataaggagga gaccaaatga
    20881 cagatgaata cttgttatca ccagaatctg gcgaaaatga actgagtttg gaaaaatctc
    20941 ttcgaccaca gctgctttct caatatatcg gacagagaaa agtcaaacaa gaacttcaga
    21001 tttacatcga agctgccaga aatcgggaag aacctttaga tcatgttctg ttgtacgggc
    21061 ctcctggatt aggaaaaacg acgatggcca tggtcattgc taatgagatg accgtaaata
    21121 ttcgtacgac gagtggccct gctatcgaac gtccgggtga cttagtagca atcttgaatg
    21181 aactggaagc aggggatgtc ctgtttatcg atgagatcca tcgattacct agagtggtag
    21241 aagagatgct ttattcagct atggaagatt tctatgtaga tatcatggta ggacaagggc
    21301 caaccgcaca tccggttcat ttccctcttc ctccctttac tttgattggc gcgacgacac
    21361 gggcaggtat gctttctgca cctttacgag atcgatttgg catcatttct catatggaat
    21421 attatgacga acaggatctg caggagatcg ttttacgttc ttcggatatt tttcaaacag
    21481 agatcattga agaaggggca ttggaaattg ctcgccgatc acgaggaacg cctagaatcg
    21541 ctaatcgctt gctcaaacgg gttcgtgatt ttgcgcaagt ccaaggagac gggaaaatcg
    21601 atcgtcctat agcggatcag gcacttactt tgctacaggt cgatcaggct ggattggatt
    21661 atgttgacca gaaattgttg cgaacaatga tcgagttata tggcggcgga ccagtagggt
    21721 taagtacgct ttcagtaaac ataggagaag agcgtgagac ggtagaagat atgtatgaac
    21781 cgtatctgat ccaaaaagga tttttaaagc gtacacctag agggcgaatt gctactgctt
    21841 atgcctatga acattttggt tacgactatc atatatgatt tttgctttta gattggagga
    21901 aaatgaatga gtgatacaac acgtacgaaa caattgatca aaaacgtatt gattgatttg
    21961 tgccaaacgc aatcttttcg aaaaatcagc gtccaaaaaa tcgcaaacga agcaaaaatc
    22021 aatcgacaaa cattttatta tcattttaca gataaatacg atttgcttcg ttgggtctat
    22081 tgtgaggatg cattgcatta tttagatcca caatgtcttt cacttgataa ttgggaagag
    22141 caagcattga aaatgctgaa agcaattgct gagaagaaag aattttatgc aaatactgtg
    22201 ctttctgacc cagaaatttt acaaaaagag ttttctcaac tgatccaaaa atcatttatc
    22261 aagatttttg aacaaatgga tgaagaaaga caactttctg caagagacaa actattttat
    22321 gctcgttttt tttcttacgg ttgcagtggt gtattagtca attggatcac gaatgaatat
    22381 gaagaaacac cgatggaaat cgctatgcag cttttccgtt tagccaagga tacggaatta
    22441 tattcttatc gtctttatgc aaaagaagag ttggaagaac agtaatagaa aaattttttg
    22501 ttggaaaagc aataatcgct aaaaggagat ggggaaatga caaaaatttt atttgtttgt
    22561 ttgggaaata tttgccgttc accgatggca gaaggcttgc tgagaaaacg aatagcatta
    22621 gaaggaaggg aagcagaatt ttttgtggat tctgctgcta caagcacgta tgaagttgga
    22681 aagacaccgc accctggaac gaaaaaaata ttaaaccaag aaaaagtaga tatgacgaat
    22741 atggttgctc ggcaaatcac accccacgat tttgaaactt ttgattggat cattggcatg
    22801 gatcaagaaa atgtagaaga actgaaacga agagcaccac gctcagcgca ggggaaaatc
    22861 catttgtttt taagtagcat tcctggaaaa gaaaaagaaa atgttcccga cccatactat
    22921 accaataact ttgatgagac atatcacttg atcacagaag gcctgaatca ttggtggaat
    22981 atttggattg atgaaaataa aataacaaat aatttgtaaa gttaacgctt tctttttaag
    23041 gcgatgttag agcatttcct tgtttttttg taaaatgtta ggtaaaataa aattaattaa
    23101 gtaacacttt cttagctaaa ggaggagtgc attcgatgaa cgaagagaca gtatcgccaa
    23161 tgaagaagct tacgaagtta gttactcata cttcgtatca actttttgat tcttttgtaa
    23221 atgacactga agaacatgct aagaatgtac gattgcaagg aaagtttatt cttgagctgc
    23281 aaaaagctgc tactgaacat agtctagtta tcttacaagt aagagcaaat cgtttgagta
    23341 gcaagtatga aacgatctca ggctgggtgg cgacaaaaac ggtcgaaaaa gaccgagtga
    23401 tgatccgcct acaaaacgaa gagcagcaga tccggatggt tcctgtagaa caaattgaaa
    23461 aaataacaat cctttcacca gcaggggaac aggaacggtt atcaaaataa tttataccag
    23521 atagaaaagc acgtatgagc ttgtaccatc aatcattact aatcaaaaat aaccgaatgc
    23581 tttaagctga tatcagtaaa acggaacgtt ttaggatgag tatcatagca tgacagcatt
    23641 cggttgatta gttgaatcga ttggatggta caagtttttt tgataaaaaa aggtcctgct
    23701 agccggggag ctagcaggat aaggagttaa aaatgaaaaa gtgttgttag ggttgtttgt
    23761 tggtatgctt atataatagt cattagttgt gaatattttg tgacgaaagt tttcgaaagt
    23821 tgttaagttt t
//
LOCUS       DAJSBL010000035        23708 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.035, whole genome shotgun sequence.
ACCESSION   DAJSBL010000035 DAJSBL010000000
VERSION     DAJSBL010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23708
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.035"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            2..74
                     /locus_tag="ONT85_001756"
     tRNA            2..74
                     /locus_tag="ONT85_001756"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35..37,aa:Asp,seq:gtc)
     gene            78..150
                     /locus_tag="ONT85_001757"
     tRNA            78..150
                     /locus_tag="ONT85_001757"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:111..113,aa:Phe,seq:gaa)
     gene            155..225
                     /locus_tag="ONT85_001758"
     tRNA            155..225
                     /locus_tag="ONT85_001758"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:187..189,aa:Gly,seq:tcc)
     gene            238..311
                     /locus_tag="ONT85_001759"
     tRNA            238..311
                     /locus_tag="ONT85_001759"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:272..274,aa:Ile,seq:gat)
     gene            323..394
                     /locus_tag="ONT85_001760"
     tRNA            323..394
                     /locus_tag="ONT85_001760"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:356..358,aa:Glu,seq:ttc)
     regulatory      583..662
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01767"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitches modulate the expression of
                     downstream genes in response to levels of
                     S-adenosylmethionine. SMK box riboswitch (aka SAM-III)
                     shares no known similarity to SAM-I and SAM-II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01767"
     gene            660..1853
                     /locus_tag="ONT85_001761"
     CDS             660..1853
                     /locus_tag="ONT85_001761"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HCR2378730.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            1983..3455
                     /locus_tag="ONT85_001762"
     CDS             1983..3455
                     /locus_tag="ONT85_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR2378731.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            3538..4326
                     /locus_tag="ONT85_001763"
     CDS             3538..4326
                     /locus_tag="ONT85_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HCR2378732.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(4393..5061)
                     /locus_tag="ONT85_001764"
     CDS             complement(4393..5061)
                     /locus_tag="ONT85_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HCR2378733.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQIMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            5176..6888
                     /locus_tag="ONT85_001765"
     CDS             5176..6888
                     /locus_tag="ONT85_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR2378734.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            6891..8660
                     /locus_tag="ONT85_001766"
     CDS             6891..8660
                     /locus_tag="ONT85_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010762530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR2378735.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENCKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFE"
     gene            complement(8865..9431)
                     /locus_tag="ONT85_001767"
     CDS             complement(8865..9431)
                     /locus_tag="ONT85_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HCR2378736.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(9431..10387)
                     /locus_tag="ONT85_001768"
     CDS             complement(9431..10387)
                     /locus_tag="ONT85_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HCR2378737.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(10387..11043)
                     /locus_tag="ONT85_001769"
     CDS             complement(10387..11043)
                     /locus_tag="ONT85_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HCR2378738.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            11413..13431
                     /locus_tag="ONT85_001770"
     CDS             11413..13431
                     /locus_tag="ONT85_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HCR2378739.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     misc_binding    13501..13707
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            13858..16272
                     /locus_tag="ONT85_001771"
     CDS             13858..16272
                     /locus_tag="ONT85_001771"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HCR2378740.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            16620..18173
                     /locus_tag="ONT85_001772"
     CDS             16620..18173
                     /locus_tag="ONT85_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HCR2378741.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(18554..20338)
                     /locus_tag="ONT85_001773"
     CDS             complement(18554..20338)
                     /locus_tag="ONT85_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR2378742.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(20353..22074)
                     /locus_tag="ONT85_001774"
     CDS             complement(20353..22074)
                     /locus_tag="ONT85_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR2378743.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            22174..22935
                     /locus_tag="ONT85_001775"
     CDS             22174..22935
                     /locus_tag="ONT85_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HCR2378744.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
BASE COUNT         7498 a         4225 c         4906 g         7079 t
ORIGIN      
        1 tggttccgtg gtgtaggggt taacatgcct gcctgtcacg caggagatcg cgggttcgat
       61 tcccgtcgga accgtttggc tcggtagctc agttggtaga gcaatggatt gaagctccat
      121 gtgtcggcag ttcgattctg tcccgcgcca ttatgcgggt gtagtttagt ggtaaaacca
      181 cagccttcca agctgttgtc gcgagttcga ttctcgtcac ccgcttttag tttttatggg
      241 cctatagctc agctggttag agcgcacgcc tgataagcgt gaggtcgatg gttcgagtcc
      301 atttaggccc atttatttta ttggcccgtt ggtcaagcgg ttaagacacc gccctttcac
      361 ggcggtaaca cgggttcgaa tcccgtacgg gtcatggaag atactttttg agtatctttt
      421 tctttttatc tgaaagaatg attttaaaaa gcttagcaga gttatcttta tggatgattg
      481 tgctaagctt ttttggacag ttctaatgta aaatattttt atatttttta agcaaaaaaa
      541 tgaaaaaata cgagcagaaa actgttttat ctgctatact tggttcgttg caagttcccg
      601 aaaggatccg caggacggag atgccttgta accgaattta actaggggga atcattataa
      661 tggtagaaag acacttattt acatcagaat ctgtttctga aggacatcca gataaaattg
      721 ctgaccaaat cagtgatgca atcttggatg caattttaaa acaagatcca acagcacgag
      781 tagcgtgtga aacgtctgta acaactggtc ttgttttagt atttggagaa atatctacga
      841 cggcatatgt agatattcaa aaagtcgtac gggaaacaat aaaagaaatt ggttatacac
      901 gtgcaaaatt cggatttgat ggagatacag cagctgtatt ggttgcgatt gatgaacaat
      961 ctcctgatat tgctcaagga gttgatgaag cacttgagat ccgggatgaa gataaaaaag
     1021 acgtattaga tgagattggt gctggtgacc aaggtttaat gtttgggttt gctgtagatg
     1081 aaacaccaga attgatgcct ttaccaatcg ctttgagtca tcgtctggta cgacgcttgg
     1141 cagatttgcg taaatcaaat gaattaacgt atttacgtcc agatgcaaaa tctcaagtaa
     1201 cggttgaata tgatgatcaa ggacaaccgg aacgcgtaga tacaatcgtt atttcaacac
     1261 agcatgatga tgcagtagat aatgaaacaa ttcgtcatga tgtcatcgaa aaagtagtga
     1321 aggaagttat tccagctgaa ttattagatg atcaaacgaa atattatatc aatccgactg
     1381 gccgatttgt cattggtggt cctcaagggg atgccggatt aacaggaaga aaaatcattg
     1441 ttgatacgta tggcggttat gctcgtcatg gtggcggtgc tttttctggt aaagatgcga
     1501 caaaagttga ccgttctgcc agctatgctg cacgttatat tgctaaaaac attgttgcag
     1561 cagggcttgc tcgcaaagca gaagtacagc tagcttatgc gattggtgtt gctcaacctg
     1621 tttcgatctc aatcaatact ttcggtactg gaacggttcc ggaagaagaa ctgattgctg
     1681 cagtgagaga aaacttcgat cttcggccag caggaatcat cgagatgctt gatttgcgtc
     1741 gtccgattta caaacaaaca gctgcttacg gtcattttgg tcgtacagat gtagatttgc
     1801 cttgggaaca tacggacaaa gtagatgcgt tgaaagcaag tttagcaaaa taagttatat
     1861 tcagctggaa cgatcgccaa gtggtggttg ttccagcttt ttttatggca gttaacctct
     1921 tgaaaaagat agtaattatt tatctatttt tagatcctgt cataatgaaa gggaggaata
     1981 agatgaaaaa agaaacaaat gttactttgg tcacagtgag tattttcatt gcaacgttca
     2041 tgacggctat tgaaggtaca atcgtaacaa ccgctatgcc aacgattgtc ggttcattac
     2101 atggaatgga aatcatgaac tgggtatttt cgatttattt gctgacaaac gctatgctta
     2161 ctccgattta cggaaaactt gcggataaag tgggacggaa accaatcttt atttttggga
     2221 cattattgtt tattatcggt tcgttatttt gcggtttatc tcaaagcatg attgggttga
     2281 ttgctgctcg tgcgattcaa gggatgggtg ctggggcaat gatgccagta gctttgacga
     2341 tcatcggcga tctttattct attgaaaagc gagcaaaagt tttaggtttg aatagttcag
     2401 cctgggggat tgccagtgtt tttggaccgc ttgcgggagg attcatcgtt gaaacgatca
     2461 gctggcactg gatctttttt atcaatgtgc cgatcggaat tctattaatt attttgattt
     2521 ggctatattt aaatgaacca aaaatcatac atgagtctaa accaatggat atttttggta
     2581 gttttagttt gatggccatg cttgtctcat tgctgttagg gttccaatta ttaggagacg
     2641 aaggaatgag tccgcttgtt tctgctttgc tgctaggtag tatcgtaatg ttaggtattt
     2701 ttgtttttgc cgaaaaaaga gctaaagatc ctgtcatcat gatgcattta ttcaaaaatc
     2761 cactatttgt tgtggtcaac ttggctgcag cattggctag tggttttttg atgggaatcg
     2821 atgtttatat ccctatgtgg atgcaaggtg tattagggct gaatgcagca attggtgggc
     2881 tggtattagc accattatca ctagtctgga tggtcggttc tttccttgca agcaagtgga
     2941 tgactcaaat gaaaactcat tgggtcttga gaattggact actaatcgca gctattgggg
     3001 gcgcttggtt gtatcttatg cctttcagaa gtaactattt atggtttttt gctatatcaa
     3061 cggtcatcgg tataggctta ggtgtgacca tgactacctc gactgtcagt gcacaaaata
     3121 gtgtatctcg tgaccagctt ggtgtagcaa cttcttttaa tacgttagtt cgaacaattg
     3181 gtcaaacagt catggtttct atttttggtt tattgataaa tggggtaact aaacgagaac
     3241 ttgcagcagc aaatttaaca aatgatgcag atatcatgaa taaactggtg aatccacaaa
     3301 cggctaaata tattgatgcg gatattttgt taccattaag aaaaattctt tatgatgggt
     3361 tacattccgt ttatctagtt ggcttgttac tcgtgatcat tgctttggtc ttaacgcttt
     3421 tcgttcatga aaaaagtggt aaaataaaac agtagtttcg gttaagatat gtacgtagcc
     3481 gaatcattca aatttaaagt tattcaaaaa taacttctta ctaaggaagg cgaaaatatg
     3541 gcatttattc aagcaaatat ttattcgaat gtgttagaaa tggaagtcaa tctaaatgtg
     3601 atactaccgc aaacaacaaa aaaggtgatt ggcaccagtt cagagcaaga gacaacggat
     3661 atacctgttt tatatttact tcatggaatg ggtggcaatc atagtgtatg ggaaagacgt
     3721 acgtcgattg aacgttatgt agcaaactac ggattagctg tcatcatgcc atctactgat
     3781 cttggctggt acacagatac agtttatgac atgaagtatt ggacattcat tgcagaagag
     3841 ctacctgaga tttgtcatga attattcccg caattaacaa gaaaaagaga aaaaacatat
     3901 gccgctggat tatcgatggg cggatatggc gcgttaaaac taggccttgc aaaatcagaa
     3961 aatttcgcag cagtaatctc tctttcaggc gctgtttctg taggaaatcg aatggatgat
     4021 cttttgatgg ttcgtaaagc atctttttgg gaagggatct ttggaccatt ggatcaaatc
     4081 gaaggttcaa aaaatgatcc gcaatactta ttgaaagaac tagtaaaaag cggaaaacca
     4141 gttccaagat tttatatggc ttgtggggaa tctgattttc tttatcaggc aaatcaggaa
     4201 atggtatctg ctatgcagga acaaggcttg gcggtcacct ttgaacaagg acctggcgat
     4261 cataactgga tcttctggga ccaatggatt caacgtgcat tggcgtggct aatggaagaa
     4321 aaataaaaaa agagtcttaa aaagatagct cctatagaga tcacaaacat agtgatctct
     4381 ataggagctt ttttactgct cgttttctct tcttttattt tgttggcata agccttcaat
     4441 ataggtttgt gcactcgtta gaaagagtgc tttttcattc aattctgcat gggactgcat
     4501 actttcttta aatccctcta attgattttc aactacataa ttacgcataa atagaactgt
     4561 ttctttcaaa aattcatttc cgtccataat tttttgtcga agctgctgtt catgctgctg
     4621 atccattaac tgtccaatca ataaattgtg gagagaggca atggcctgat gactttgggt
     4681 gactgaaaat ccttcacgaa caagtaattg gatcatttgc tccaaatgtt ctaatcgact
     4741 cggaaaagcg ggcacagtat tgatttctaa ttctgcccca caagggaaac gtgtataaag
     4801 gtcatagtaa ttttccatat actgcagcaa ctgttcattc caaggcaaag ttggatctgg
     4861 aagcgctaat tcttcttcca ttgtttcagc cattatttgc aagagctgtt gtttgttttt
     4921 gaaataccaa tataaagcag gtgcttgaac atgaattctg tttgcgatgt ttcgcatgga
     4981 aagctgcgaa agagcttgtg tttcagccag gatatcaaag gcggcctgaa tgatgattgc
     5041 ttgagaaagc tttatttcca ttttatcaac ctcgtgtatt gcattatctt tttaaacatg
     5101 gtaaattgtt agtcggtcta attttacagt gttaaattag tcctgtcaat ttaaacaaag
     5161 gggtgtaatc aacgaatgaa attaatctta cgacatgcga aaaaatataa agtagctgtt
     5221 tttatttcct tgctgtccgt agcagtcatg gttgcagcag cattatggca acctaagtta
     5281 ctacagcaag tattggaagc tattattaca gaagataatg atgaaatgaa aacaattggt
     5341 atttacctga tcagtctggc tttgctagga ttagccgcag gtgtaacgaa cacgattttc
     5401 tctgcaaaag tagctcaagg cgtaagtgca gatatccgag aagaagcatt tcgaaagatc
     5461 cagacatttt catttggaaa tatcgaacag ttctctgctg gaaatcttgt tgttcgtcta
     5521 acaaatgata tcacgcaaat ccaaaattta gtgatgattt ctttgcaatc actttttcga
     5581 attccattct tatttattgg tgccttcatt ttagctatga tcacgatgcc gcaattatgg
     5641 tggatcatcg tagcgcttat cgttacggta tttctgatta cggcactttc atttacaagg
     5701 atgggcaagc acttcatgat cattcaaaaa ctgattgatc gtgtgaatag cattgcaaaa
     5761 gaaaacttac tagggattcg tgtagtgaaa tcttttgtac aagaagaaaa ccaattgaag
     5821 tcattcagca aagtaagtga ggacttaaca aaacacaaca tcatagtcgg tacattattt
     5881 tctgtgatga tcccgtcctt catgctggct gccaatttgg ctgtggttgg ggcgatcttt
     5941 tttgtgagcg atcttgttaa agatgatcct acgttaattg gtggaattgc ttcattcatg
     6001 aactacttga tgcagattat gatggcaatc attattggcg gaatgatgat gatgatgact
     6061 tcaagagcag ccgtatcgct gaagcggatt gcggaaattt tagatgctga accagatctt
     6121 acttatttag acgttccaga acaagagttg acgggttctg tcacttttga ccatgtttct
     6181 tttcgttatc ctggtgagga tacagatacg cttaaagata tcagcttttc tatcaaacct
     6241 ggagaaatgg ttggtatcgt cggggcgact ggtgccggga agtcaacact agcacaattg
     6301 atccctcgtc tatttgatcc gacagaaggc aaagttgaag taggtggtgt agatcttcgt
     6361 caagtaaatg aaaagagttt aagacaaacg gtttcctttg ttttgcaaaa agcaatttta
     6421 ttctcaggaa cgattgctca aaacttgcgc caagggaaaa agaatgcaac agaaaaagag
     6481 atggaacatg cttcttctat tgctcaagca aaagaattca tcgaaaagtt atctgatcgt
     6541 tacgaagcac caatagaaga acgtagcagt aacttttctg gtggtcaaaa gcaacgttta
     6601 tccattactc gtggcgttat cggtaatcca aagatcttga tcctagatga cagtacgagt
     6661 gcactggatg ctcgctctga aaagctggtc cgagaagcac tcgatcgaga tttgaaggga
     6721 acaacgacga ttgtcatcgc acaaaaaatt gcttctgttg tcaaagcaga ccgcatctta
     6781 gtattggatg aaggcagatt agtcggcgaa ggaacccatg aggaattggt agctggaaac
     6841 cgtgtctatc aggaaatctt tgaaactcaa aaaggaacgg aggagtaagt atgactgatc
     6901 taataaaagc aagtaaattt ttctatcact atttgaagcg gtacaaactc tcctttttct
     6961 ttatctttat cacagtgatc attgctacct atctacaagt aaaggcacct caatacatcg
     7021 gggaagcttt tcaagaatta gctaattacg taggaggctt gatgcaaggc gtagacgata
     7081 aaagcaaatt tgtttctgta atatggaaac tcttgctgtt ttatgtacta gctagtgcag
     7141 ccaactttat ctatagtatt ttgttcacac aagtagtagg gaagtcaacg aaccgaatgc
     7201 gtatcggtct attcaacaaa ttggaaaaat tgacgatccg tttctttgat tctcatcaag
     7261 acggtgaaat tttaagtcgt tttaccagtg acttagataa tattcaaaat agtttgaacc
     7321 aagctttgct tcaagtcatc acgaatgctg ttttacttgt cgggatcttg atcatgatgt
     7381 tccgccaaaa tgtagagtta gcttgggcta caattgcttc cactccagta gccgttttga
     7441 tagctgtctt gattatccgc aaagcacgga aatatgtgga tcttcagcaa gatgaagtgg
     7501 ggaaactaaa tggttatatg gatgaaaaaa tcagcggaca acgtgtcatc atcacaaatg
     7561 gcttacaaga agaaacgatt gaaggattct tagagcataa tcaaagtgtt cgcgaagcaa
     7621 ccttcaaagg acaagcttat tctggtctac tatttccgat gatgcaagga atgtcactgg
     7681 tcaatacagc gatagttatc tttttcggcg gctggttagc attaaatggt gatttggaac
     7741 gttcggtggc attaggctta gttgtgacat tcgtccagta ttcacaacaa tattaccaac
     7801 cactgatgca gatttcatct ggatacagca tgatccaatt agcgattaca ggggcaagac
     7861 gattgaatga aatatttgat gaaaaagatg aagtcaaacc agaaaactgt aaagcatttg
     7921 atggtgttga aaaaggtctt gcgttgcagc acgtagattt tggttattct ccagaaaaac
     7981 tagtcttgaa agatgtctcg atcaatgtgg acaaaggaga aatggttgct cttgttggtc
     8041 caacaggatc ggggaaaacg acgatcatga atctgctaaa ccgtttttac gatgtcaatg
     8101 atggttcagt agtcatcgat ggaatagata tccgagatta tgatcttgat gcattacgta
     8161 gacatgtagg aatcgttttg caagaatctg tattgttctc cggaacgatc aaagacaata
     8221 tcacattcgg caaaccggac gcttcagatg aagaagtcat caatgcagca aaacaagcga
     8281 atattcatga cttcatcatg acgttagaaa aaggttatga tacagagata tccgaagaaa
     8341 ataacttatt cagtacaggt caaaagcagt tgatcagtat tgctcgaacg atcatcacaa
     8401 atccatcttt attgattttg gatgaagcaa caagtaatgt agacacggta acagaagcaa
     8461 ggatccaaaa agcaatggat gaagcaatca aaggaagaac cagctttgtg attgctcacc
     8521 ggctgaaaac aatcttgaat gcagatagaa tcgttgtctt gcgtgatggg gaagtcattg
     8581 aagaaggcag tcatcacgag ttattagcag aaaatggatt ttatgcggaa ctttatcata
     8641 atcaatttgt atttgaataa agaggttgtg aaaaagctgt cctgcatcaa gtaataagaa
     8701 cagccaatca aaaacagctt ctcatgtttt tgatttggtt ggacttatta ccgcagatat
     8761 aagcttttga gcaccgtgaa taaagaggtt atgaaatcaa cgctctaacc tgagtattag
     8821 agaaaaaaac gcaaaacata tcatttatgt tttgcgtttt ttatttatct ttttttctca
     8881 atacaatata aaataggcgg ctggttcttt tggttgataa accgataatt cagcacatta
     8941 tatgtttcct gtggcagttt ggaacaaaaa gaatcaacca tatccagttc tttttctcca
     9001 ccttcatgac catagtagac gacaaggatc aatcgtccgc gaggcacaag tcgtttcaat
     9061 atttcttcca tagctgtgcg tgtagtctct ggcatagtga tgattgcttt atcacttttt
     9121 gggagatacc ctagattgaa aatccctgct ttgagatttt gttgttcagc tatcacttct
     9181 cctaacgttt cgtggccttg tagaaacaat gagacacgtt cttctaactg acgctgttct
     9241 agcctttcac gagtagcatc gattgcttgt tgctggatat caaaactata gacatgacca
     9301 gtttttccga tgtgttctgc tagaaaaacc gtatcgtggc cattgcccat cgttgcatct
     9361 acgacgtgat cccctggttg caagatttct tgcagtaatg tatggctgaa atgcaaagca
     9421 gtttgcaaca tcagacgctc acctttttcc gtacatcata ttttccttgg aagctatctc
     9481 tgcgaagaag ttcttcatca attgcattca agacttccca ttttttcaag ctccacattg
     9541 gtccaatcaa ggaatcccac ggcgcatccc ctgtcaaacg atggatgatg atctcttttg
     9601 gtatcatttc cagctggtcg caaatgacat tgacatagtc aggacggctc attaattgaa
     9661 ggcgaccttc atgatagtcc cgcaacattc tcgttttgcg catcagatgc atcaaatgga
     9721 gcttgattcc ttggatatct gaatctaaaa tggtgcgtct gacattttct ttcatcattt
     9781 ctaaactttc gcctggtaat ccgttgatca aatgcgtaca aacacggata ttatgtttgc
     9841 gtaatttagc cacgccatct aaataagttt gataatcatg tgctcggttg atcagttttg
     9901 aagtttcttc aaaagttgtt tgcaatccaa gttctaccca taaatacatc cgctcgttca
     9961 gttcagcaag gtaatcaact acttcatctg gcagacaatc cggtcgtgta ccgactgata
    10021 atcctacgac acctggtaaa ttgactactt gctcaaatcg ttctttgatt atttcaactg
    10081 gcgcatgggt gtttgtaaaa ttttggaaat aaacgatgta ttgttggaca tgaggccatt
    10141 tttgatgcat catgtcgatt tctttctgga attggattgg aagagggtct tcaggtgcaa
    10201 cgatcatgtc tcccgaacca gatacactgc aaaaagtaca accgccatgg gcaacagtcc
    10261 catctcgatt cggacagtca aatcctccat cgatcggcac cttgaaaatt ttcccgccaa
    10321 actcatttcg taaagcgtaa ttccaagaat gatatctttt atttccttct gaataaggaa
    10381 aagtcattaa aagtccttct ttctgtaaga gttatctatt tgttttcttt tttctctata
    10441 cactggataa gtcaggcaca gccaagaaag actgagtaag taaccgccga tgatatcagt
    10501 aggaaaatga acgcccagat aaatgcggct tatcccgaca cttactataa taaacatcat
    10561 cagcaattga agcaatgtgc gtaaggttct tttttgaaca aaagctggaa gcagaaatag
    10621 taaagaaccg taaagaatca tactgcctgc tgcatgtcca ctagggaaac tgtaacttgt
    10681 ttcaactact aagtgcgcca atgaaggacg ctctcgcatg aaaaatagtt tgatcaccgg
    10741 gttgatgatt ccagcaatta caataaatcc agaaacgaac caaaatgttt ctgccctttt
    10801 tttcttatac cacatccata aagccgcaca agcaaataaa atgatgacgg taagaggatt
    10861 accgaattta gtcataaaga gttggtagct gttcagatca ggataagaag ctctgacaat
    10921 gcctgtaaac cactgatcga aaccagaaag ccacgacaga tggaacttta cgatccagcc
    10981 caaaatgaaa aacaggacaa gaaagaagct accagccacc tgaaatgatg ttttatattt
    11041 catacaattc ctcctttgaa gcaatgactt attatagcat ttttggtcca gcgaaaaaaa
    11101 cggtaaagag ataaaaccaa tgtatgattc ctctttgaat aaccaatatt cttataaaaa
    11161 aaataactgt aatcggaaag acattttttg gtaatgcaaa tgaaatgtta gggaaataaa
    11221 atcagtgttt ttcgaaatcc ttttaaacaa gaacgtagac gtaggaagaa cttgaaaatg
    11281 aacagaagtt gttcattacg gtaaattaca atatgattac aaaaaacaac aaagctctta
    11341 aataactttt aataaatatt aagagaagtt ataattatta ataatagata aattgaagtt
    11401 ggaggataga agatggaaaa cattgcaaga aaagaacgga gacgtttaga acagaccaaa
    11461 cgttttcgaa aagcaaaacg aggggcagca atcgtgggaa ctgcgatggt aggctgttct
    11521 gtcgctgccc cattagtaca gccggttcct gttcaagcgg atgaaacacc tgttcagttc
    11581 ggcgcaagaa tcaatacagc ggcatttatc acagaaattg ctacttatgc tcaaccaatc
    11641 gctcaagcca acgatctata tgcatcagtg atgatcgcac aagcagttgt agaaagcggt
    11701 tggggcagta gtgcgttatc tcaagcacct tattataatc tttttgggat aaaaggcagc
    11761 tatcaaggac aaaccgtcta catggatacg cttgaatttt taaataatca atgggtaaat
    11821 aaaaaggagc ctttccgaca atatccttct tttgctgaat ctttcaatga caatgcctat
    11881 gtacttagaa atacttcatt tggcaacggt tattactatg ccggtacttg gaagagcaac
    11941 acgaaatcct atacagatgc gacagcttgt ttgacagggc gttatgcgac tgatcctggt
    12001 tacgcaggca aattaaataa tatcatcgca acttacggat tgacccaata tgatacacca
    12061 gctagcggaa acgcaggcgg tggagcgact acaggtggca ataatactgg aagtgcaggt
    12121 agtactggcg gttcaagcaa ttctggtaat acaaactcag gttcagcaac ttcgaccact
    12181 tatactgtcc aatctggtga ttctgtttgg ggaatctcaa ataaattcgg tatcacaatg
    12241 gatcaattga tccaatggaa caacatccaa aacaatttca tttatccagg acaaaaactg
    12301 acaatcaaag gtagccagga aaatggctct tcgacgaata attccggtaa caataccaat
    12361 tcttcaggaa atgctggtac atcaaacgga ggacaaacaa ctggtgcgaa atacactgtt
    12421 caatctggtg attctgtttg gaagatctca aatgatcacg gaattacgat ggctcaattg
    12481 attgaatgga acaatatcaa gaacaatttc gtttatccag gacaacagct aatcgtatca
    12541 aatggcggtt cttctaatgc tggagcaaat actggctcaa atactgggaa tacaagtggc
    12601 tctagttcgt ccagctcaag ttcttctaat acagcttcag gaacgaaata tacagttaaa
    12661 gccggagact ctgtttggag cgtctcaaat aaatacggaa ttacgatggc tcagctaatc
    12721 caatggaaca atatccaaaa taactttatc tacccaggac aacaactgac tgtttcaaac
    12781 agtggatcaa caaacagcac gtcaggtaat acaggttcgt caaccaatac aaataacagc
    12841 agttcagtta atactggcag cagttcgtct caaggcactt ataccgtgaa agcaggtgaa
    12901 tctgtttgga gcgtggcaaa taaaaacggc atcacgatgg atcagttaat cgaatggaat
    12961 aatatcaaaa acaacttcat ttatccagga cagcaattga tcgttaaagg tggaacttcg
    13021 gcaaatacaa atacaggttc aactacttct gcagcgaaac caaatacacc gaatacgtct
    13081 gctacgacaa gtacatcatc cactggtaat acgatgtata ccgtgaaagc gggcgagtca
    13141 gtttggagcg tagcgaacaa acatcatatc acgatggatc agttaatcga atggaataat
    13201 atcaaaaata atttcatcta tccaggacag caagtgattg tgaagaaagg ttcatctcaa
    13261 tcaacaaacc aacaatcagt tactggttcg aaaacctata ctgtgaaatc tggagaatct
    13321 gtttggagtg ttgctgacag tcatggaatt acaatggctc aactaatcga atggaacaat
    13381 attaagaaca actttattta tccaggtcaa acgttgattg tagctaaata aaatagctgt
    13441 tcgaagaaat aacgattgaa ttccacaaaa atatttgtta ctataaggct acttaaatat
    13501 gacaacactt gaaaaggaag tagtaaaaag actgaagttt cagagagcgc atggctggtg
    13561 agaatgtgca cggatgcttt tgaactcgcc tttgagttgc ccttgctgaa tacccgagta
    13621 agcaagcggc ggtgacggcc gttacaacgt tcgaggcctt ttcactaagg taaaattagg
    13681 tggtaccgcg tagattacgt cctataaaaa gagcaatctt tttataggat ttttttattt
    13741 cttttgtgaa gtttgagaaa acaaagcaaa aggaaataaa aataaacaga ttctgccggt
    13801 tttttcgtta tatttataat agaaacaagt gttttcaata gaacaggagg acagcaaatg
    13861 agttacaatc ataaagaaat tgagaaaaaa tggcaaaaat actgggcaaa aacgaatact
    13921 ttcaacaccc atgatgatcc tgaaaagcca aaattttacg cactggatat gtttccctat
    13981 ccatccggtc aagggctaca tgtagggcat cctgaaggtt atacggcaac ggatatcctt
    14041 tcacgtgtga aaagaagtca aggatacaac gtgctgcatc caatggggtg ggatgcattc
    14101 ggtctgcctg ctgaacaata tgcgctagat acaggaaacg atccagctga gtttacccaa
    14161 aaaaatatcg aaacattccg acgccaaatc aattctttag gatttagtta tgactggaat
    14221 cgcgaagtga atacaactga tccggagtat tataaatgga cacaatggat ctttacgaag
    14281 ctttatgaaa aaggtctagc ttatgaagca gaagtagcgg taaactgggt acctgaatta
    14341 ggaacagtta tttcaaatga agaagtgatc gatgggaaaa gtgaacgtgg tggctatgat
    14401 gtagtcagaa aaccaatgcg ccaatggatg ctgaaaatca ctgcttatgc tgatcgctta
    14461 ttggatgatc tagaattagt tgattggcca gaaagcatta aagaaatgca aagaaattgg
    14521 atcggtcgtt cagtaggagc aaatattgaa tttaaagtgg ctggtacgga taaatcctat
    14581 accgtattta cgacacgacc agatactcta ttcggtgcaa cttactcggt tcttgcacct
    14641 gaactagatc ttgttagaga aattacgacc cctgaacaaa aagctgctgt agaagcttat
    14701 atcgaagaga cagctaaaaa atcagatcta aagagaacgg atcttgcaaa agaaaaaaca
    14761 ggagtattta ctggtgctta tgcaatcaat ccagttaatg gcaaggaaat cccaatctgg
    14821 atcgccgatt atgtattatc ttcatacggc acaggagcca tcatggctgt tccagcacat
    14881 gatgaacgtg actttgaatt cgctcaaact ttcggactag aaattcttcc agttatcgaa
    14941 ggtggagatg ttgaaaaagc tgcttacaca gaagatggta cgcacatcaa ctctgaattt
    15001 ctagacggca tgaacaaaca agaagcaatc gataaaatga atgaatggct agaagaaaat
    15061 ggcgtaggga aaaaagaagt cagctatcgc ttacgtgact ggctattctc ccgtcaacgt
    15121 tactggggtg aaccaatccc aatcatccat tgggaggacg gaacagtcac agctgtccca
    15181 gaagaagagc taccattacg gttgcctaag acaaaaaaca tcaagccaag cggtactgga
    15241 gaatctccat tagcaaatat cgaggagtgg gtcaatgtcg ttgatcctgt aacaggtaaa
    15301 aaaggccgtc gcgaaacgaa tacaatgcca caatgggcag gaagctcatg gtattacctg
    15361 cgttatatcg accctcataa caaaaaagaa ctagcagatt atgaaaagtt gaagcgttgg
    15421 ctaccagttg acatctacat tggtggagca gaacatgcgg tacttcattt actatatgca
    15481 cgattctggc ataaattctt atacgatatc ggtgttgttc ctacaaaaga accattccaa
    15541 aaactataca atcaaggaat gatcttaggc gaaaacaacg aaaaaatgtc aaaatctcgt
    15601 ggtaatgtag tcaatcctga tgatgtggtc gaaacttacg gtgcagatac actgcgcatg
    15661 tacgaaatgt tcatggggcc attagatgct tcgattgctt ggagcgaaaa tggcttagaa
    15721 ggaagccgta aattcttgga tcgtgtatgg cgtttgatcg ttgatgagaa caataaaatg
    15781 cgtgaccgta tcactacatt gaatgatggc aagcttgata aagtgtatca tcaaacagtg
    15841 aaaaaagtga cagaagacta tgaaaatctt catttcaata cagcaatctc ccaattgatg
    15901 gtctttatca atgaagccta taaagtggac gctctgcctt atgaatatat cgaaggattt
    15961 gttcaactac tcgcaccgat tgctcctcac atgggtgaag aactatgggc aatccttgga
    16021 aatgatggtg gtatttctta cgcaccatgg ccaacatacg atgaagcagc acttgttgaa
    16081 gacgaagtag aagttgtttt ccaagtaaac ggtaaagttc gtgcgaaaag caatgttccc
    16141 cgagacttag gaaaagatga gctagaaaaa gcagcattag ccaatgaaat agtgcaagaa
    16201 tatattgaag gcaaaacagt cagaaaagtt attgcggtac caaacaagtt agtcaatatc
    16261 gttgctaatt aattggctct ttgtcaataa ggactgatga gttgtgcaaa atcaaatctg
    16321 ggccagaatg aacttcattc tgtctcagat tttttgttat ttcaaattga ttaattgatt
    16381 gatcaaaaag gttttgattt tcatgttctc aaacacgttc aatgtctttt tcttctgaat
    16441 ggtcttgtaa gagatctctg taatcatcaa atttttgtct attaatctta gtattttttg
    16501 atgtataaac tacctgaaat tgagagtaca taccaaattc ataaattttt tattagtggg
    16561 actagtgatt gtatggattt tgacgcataa tgaatgaagt atgagaaaag gagagacaaa
    16621 tggatcaaga actatttatt atcgaagtat tatcagctaa acaaaaaatt atcgagaaag
    16681 cgaaaaaaag agcgaaaaga ataatgttca agcttttaag cttagtattt ttctctggtt
    16741 tgcttttttt cggagctgta tcgttcctat atcgccatat gagtgcagta gtccgttcag
    16801 ctgaagggat gcaaaaatta aataatttgt atcacttttc gtggggagtt ggcggaataa
    16861 caattgttgt gcttattgtt tttcttctac ttatttggct ccgttaccat caattgtcgc
    16921 gtcatttcga tcaatggctt agtcaaagag cacaacagtt tttacataat aaaccaactt
    16981 ctcaagatga tgactatctt tattttgaga agacttcgac gagtcaagct tttcggatac
    17041 aaaaaagtga ttgccagtta ttgctgagat cacttgatga tttacctatc tacttaggcg
    17101 aaagcaaatc aaaattcgga atgaacacat tccatatttt tttactagaa aacgaaccca
    17161 gatgggaaaa aagccagtct tcgcctggtg gatacctgac aactaagatg agtacattag
    17221 tcactggttt tttcctcatt cttttagctg gaacaatcta ttatgggaga tacactcagc
    17281 caaaaatgtc ttctcatata aatgctgctt ctcacatgac aatgaactca actaacgata
    17341 cgcatataca aaagacaaat gaagggttgt tagctgatcc taagcaaaag gtaaatttga
    17401 caacacaaaa gataaatgat ctcaaacttg atacagagac gcactctctc tatatgacaa
    17461 cagatagtgg tgctaattgg gtatttgtgc cgattgattc gcaatggttg cgttttggcg
    17521 aatacaccct gacaacgggg ctgattcctg aaggttattg gatggacaac acatatgata
    17581 tttccgccga tttctcatgg tttatctatt cttctgggga tgatgtacgt ctgctgactt
    17641 caagagataa tggtaaaaca tggcaaacgt ctattatcac ccaatcagtt cagccgatac
    17701 gttatcgaaa agcggtattt tatggcaacg atggtctatt tatctgtaca tcagccagtg
    17761 ggatgtctgc agaaactttg tacatttaca ctactatgga tggcggaatc acttggataa
    17821 aaagtggaag tacaatgatc aatcagccag tacaaaatgt cagcttcgtt acaacttctt
    17881 taggttttgt ttcgacacgt gaaagtttgt tttatacaaa caacggagga cgctcattca
    17941 aagaatcagt tctgtcgatt ccggaaaact atcagacagg ggggatagat ctttttcaat
    18001 caccaaatga agttactcaa ataagcgcga atacgttaga gacaaaattt tatttgttga
    18061 aaaaaggacc aatcgatgca gggaaaatgt ttgcctgcct cttccgttca actgataacg
    18121 gggaaacatg gcaattcgtt cagcaactat cgcaagtaga acaaggagat taaagaagag
    18181 agattgtgag cctgacgttc agcaccgagt cacaagaaac aaattcaaaa aacagcttct
    18241 catatttgtt gaatagtgta actcgtgatt cggagttgac aagagcaaca agaagcgatt
    18301 ttgaagtata gcttctcgta ttttacaaga tcgtgtctta ttgccgatag gttgtctctt
    18361 gaacaccgtt tattcgtaaa aagggattgt gacaagtctt ttgtcactac ttaccgaacg
    18421 aaactgaagg agctatccaa aaagtatcat tagtattatt ctttgtgttt ctagtggcac
    18481 cactagtaca tttaaaaaag ctatcgatca tggactaatc atacatgatc gatagctttt
    18541 tagataatgt ttgttacgct atctgaaatt gactattata aagactggca taaagcgttg
    18601 gtgattggag tagttcagaa tgtgttcctt tttcgacgat atcgccattc ttcattacaa
    18661 ggatcatgtc tgcattttca atcgttgaca aacggtgtgc aatcacaaaa cttgtcctat
    18721 cttttgtcac agcttccatt gcttgctgaa tcaatgcttc tgttcgtgta tctacgctag
    18781 aagttgcttc atctaaaata acgacaggag gattcgctag aatgactcga gcaattgtca
    18841 ataattgttg ctgtccttga gataacgtgc cgttttcgct agaaatgatc gtatcatatc
    18901 cgtctggcag tgttcgaata aagtgatcac attgtgcgat ttcagccgct tcgataattt
    18961 gttctcttgt ggcatccatt ttcccgtagg cgatattatc agctaccgtt ccttcaaaca
    19021 gccaggtatt ttgtaaaacc atcccaaaag aacgacgtag ttctccacgt gaaagcttgg
    19081 taatatcaag accgtcgaat tcgatctttc ctttattcag ttcataaaaa cgcatcagca
    19141 gattgactaa agtcgttttt cctgcaccag ttggaccaac gatcgctacc gtttgttttg
    19201 gctttactga aaaatcgact tgattcatca atattttatc tgatgtgtaa ccaaactgta
    19261 catttttgaa agctattttt cctttcggtt gttctaaaag tcttgcatct ggaaggtctg
    19321 gaatttcatc tgtttcatct aatatctcaa atatcctatc gattgctgcc attgcggatt
    19381 ggatcgagtt gatcacataa gaagcggtag aaattggttc agaaatctga ttgatgtact
    19441 gaagataagc ttgtaagaag ccgatcgtca caccacctga tagaacaagc atagcaccga
    19501 ataccgcact tatgatgaag gcgagttgat taatgaaacg aatggccgga tagatagcac
    19561 agttcataaa ttgagcacgt ttaaaagcgt tgtattgctt tttatttact tcgtgtatag
    19621 cagcttctgc atgagcttgc tgattgaatg ttttgaccac tagattacca gctaagattt
    19681 cttccattcg attatttaaa acacccagtt catactgatt ctgttcagaa atctgtttgt
    19741 ttttgttcgc aatcttagtt gtaacaattg aactgccaac gatcagcaat aagacgagca
    19801 aagtaagctt gctttcgata taaaacagca tcagaccagc agcagctatt gttacgacag
    19861 aagtaaaaaa ttgattgata cctgtcaata acacttgaga aagttgattg agaccggtag
    19921 ttgtccgact aatgatgtca cctacttgat gattgtcaaa aaacgacatc ggtaatgttt
    19981 tgaatttttt tgtgatttct tttcgcaccc tcaatgtgat cttttcgctt aacgctgcca
    20041 ttgtccgttc ctgaataaat gaagtgatgc tgcttaagac tgaacagccg atcagcaata
    20101 agacagggaa caacaatgct tgtttgatat cactgaaaga aagctgtcca atacctgttt
    20161 ttcttatttc tgataagaga tcatcgatcc cgattcccat gatcaagggc atggctacga
    20221 ctaataggtt cccgaataaa ctgcatagaa acaatccgta aaaatattta tgttcaggtt
    20281 tgatcatttt ccaaaaacgt atcaaagcac gcgtgttcaa cgtttttttc ttttccatcg
    20341 tatcccttct ttctatattc cttgtgattt tgcaaactcc tgataagttt ggcaattaga
    20401 cagtagttct tcatgagtac cttgtccaac gatttttcct tcatctaaaa caatgatgtt
    20461 atctgcttgt tggattgtac tgaggcgttg agcaacaatc acgagagttt tctccgacat
    20521 gtgtttatga agggctgctc ttaatgctgc atctgtttta tagtctaaag cggagaaact
    20581 atcatcgaat acatagatct ctgctggttt gatcaacgct cgagcaatac atatccgctg
    20641 tttctggccc cctgaataat tagaaccgcc ttgagcaaca aagctgtcca atccgtcagg
    20701 aagggtgtcc actagttctt ctaattgagc gatcttgatc gctttagaca actcttctgt
    20761 tgaggcatat tcatttccca tctgcaagtt gcttaaaatt gtcccgctga ataagaaagc
    20821 cttctgaggg acataactga tgtgggagcg gatctgttct tgagtcattt gagtaagagg
    20881 aataccacca agggttactt ttcctatcgt agggtcacta agtctcaaga gaagtttcgc
    20941 caaactactt tttccagatc cggtcccgcc aacgatcgct gttgtttttc ctttaggtat
    21001 tgtgaaatca atatcttcta gaactggatc agagatttca gtatatttaa atgtgacatg
    21061 ttcaaaagca gctgcatttg tctgatcaaa ggttggttca gctactgttt tgatcgtctc
    21121 ttcctcaatt tcgatctctg cttgcagtat ttcttctatc cgtcgtaatg aggcaaacga
    21181 tttagggata atgaccaata ccatagctgc gatcatcaag taagcaagtg tcagcataga
    21241 gtattcgact acagcagtca cgacaccgat ttgtaatgct ccttctgcta caagatttcc
    21301 gccaaaccac aaaatagaag cataaacgat tcccatgagc aaaaaagcaa ttggagtgac
    21361 ccaagcaaat attcggttca ctttgatcat ttgattagca tagtcagcaa agctcttatt
    21421 cgttcgcttt tcttcatatg attgattatc aaaggcacgg atcatattaa tcccagtaaa
    21481 gaattctctc aatgtgatgg tgatccgatc catttttggc tggatactca aagataaagg
    21541 tgcccctcgt ttcatcagtc gataaacaat ccatacataa aaaacaacag agatcaatgt
    21601 aacccatgtt agtaatagag aataacgaaa catcatgaga atggtgaaaa cgcaaataac
    21661 cggtgctggt aagatcattt ggcaaaataa aacaatcatt tgctggacat tatcgacatc
    21721 attcgtcatt cttgtcaaaa gagatccaga actaaaacga ctgatatcct tcattgacag
    21781 ctgttggaat ttacggaaat atttctcccg cgtttggaat ccaaacctag cggaagccaa
    21841 tgctgaaaag taactagagg caactgcagc gattgttcct agaaacgcta caagcagcat
    21901 ttcaactcca atttttttta taactgaggc ttcgccactt gctatacctg tgtcgatcag
    21961 ctttgctact aattttggta cacctaatgt accggcgatt tctaaacaga tcatcacaaa
    22021 ggtaaggaga gcgagtcctt tgtttttctg taagaatgtt tttaagatag acatattatt
    22081 cctccttttt tcctgatact tttcgattct ataccttcaa gtaacttgaa ggtcaataga
    22141 aaaaagaata aactgaaaaa gaggtgagaa aaaatgaaaa aaatgtatac gattggccaa
    22201 gtatcggaac tatttgattt gccaaaatca acgatccgct attgggatga acaaggatta
    22261 atccgttcaa gtagacaaga agagaatgat tatcgattgt ttgatatcga tgatattttt
    22321 atgctttatg atatcgtttt ttatagaaaa ttagatatac cgatcaaaca gatgaagaat
    22381 ttatatggaa aaacattgac ggagttatat gagacacttg atgaaacaga aagaagaatc
    22441 cataaagaac tggtggtgat gaaacaaaaa caaaaagaga taagggagca aaaaaaacag
    22501 ttgaaattga tgatcgatac aaacgaggaa gaatttcctg tagaagagat cccttttgac
    22561 tgtatgatct caacggagtt tgaagatatt gtggaaatca aaaaatttct acctaactat
    22621 tcaagctttg ggatgatgag tatgccagct tccagttcac agacgatgta tggatttttt
    22681 atcgacccta gtgaagtaca tttatttact caagacgtga tttgggagaa aaaagatacg
    22741 gctgtttatc gtcgattctt attgaaatcc gagatgaacc atgcggaacg taataatatt
    22801 ttagaaatca gagaaagaat gtatgaaaaa ggatggaaaa caggagaagt gatcggccag
    22861 tatctattga cgaatacgga tgaaaacaat atcagaactg aatattatca tgcatggata
    22921 gaaatgaaaa aataggaaat atgtgtttaa gataactcga aaatgatggt tgttttagtt
    22981 ataaagaaaa attttctggt tcgattacgg gtttaatgtg ggggattatt agtcgtggat
    23041 agatagaatt ttagtttttt agaggaaata tgtctttaaa aatggaatgg ttgcaattct
    23101 tagcgatata tacgatgtaa ttgtcctctt aaaaaagtat ggaaaaataa ttgtattggg
    23161 tgttatgtat aattcatggt gaactgagac taatgagaag aaaaattcat attgttatga
    23221 gtgagattgt atcagatgag aatgataaat taaataggtg cataaaagca ttacacttaa
    23281 aatacattgt tgcttacatt attacaatat gttaataagg caaaaataaa tagatggatt
    23341 gaataaacat attttttaat caaatagtcc ctactgtgac tttcctcagc tatcggtgat
    23401 actaccgata gcattttttt gcttataaaa taattataag tatttagaga tttttcttct
    23461 aacattcttc aaacaagaaa aatcacaaag gttattgtta tttttatata ttctttttaa
    23521 gaatgattgt ttttttatag aaattttaaa aaataaaaca cgttattgat ggatagttag
    23581 tttgttattt ggtaatatta tagaagttga ctggtagtga tttatttttc gtcgttttat
    23641 aaaagtgtta ttttttggaa aaattcacaa atatgtaacg aatgtttttc tggaatttgc
    23701 tgtgttaa
//
LOCUS       DAJSBL010000036        23076 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.036, whole genome shotgun sequence.
ACCESSION   DAJSBL010000036 DAJSBL010000000
VERSION     DAJSBL010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23076
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.036"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            102..2543
                     /locus_tag="ONT85_001776"
     CDS             102..2543
                     /locus_tag="ONT85_001776"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HCR2378745.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
     gene            complement(2873..3577)
                     /locus_tag="ONT85_001777"
     CDS             complement(2873..3577)
                     /locus_tag="ONT85_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2378746.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
     gene            complement(3764..4009)
                     /locus_tag="ONT85_001778"
     CDS             complement(3764..4009)
                     /locus_tag="ONT85_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2378747.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            complement(4026..4877)
                     /locus_tag="ONT85_001779"
     CDS             complement(4026..4877)
                     /locus_tag="ONT85_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HCR2378748.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            5043..6047
                     /locus_tag="ONT85_001780"
     CDS             5043..6047
                     /locus_tag="ONT85_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HCR2378749.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
     gene            complement(6288..6644)
                     /locus_tag="ONT85_001781"
     CDS             complement(6288..6644)
                     /locus_tag="ONT85_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HCR2378750.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
     gene            7012..8199
                     /gene="tuf"
                     /locus_tag="ONT85_001782"
     CDS             7012..8199
                     /gene="tuf"
                     /locus_tag="ONT85_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCR2378751.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            complement(8387..10978)
                     /gene="mprF"
                     /locus_tag="ONT85_001783"
     CDS             complement(8387..10978)
                     /gene="mprF"
                     /locus_tag="ONT85_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HCR2378752.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(11004..11765)
                     /locus_tag="ONT85_001784"
     CDS             complement(11004..11765)
                     /locus_tag="ONT85_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR2378753.1"
                     /translation="MTDIIVILYTVGDYVKKEKKYAKMPDGSEIYYEKSGQGFPLFLL
                     HGNDGSGRFFSEQVPVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEK
                     IDQADFLGFSDGANLALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVW
                     LLSLFRPSLRKNLLVIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKT
                     IPKASFVLVKEQGHELARKDPERFNREVLQFLSET"
     gene            12043..12564
                     /locus_tag="ONT85_001785"
     CDS             12043..12564
                     /locus_tag="ONT85_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
                     /protein_id="HCR2378754.1"
                     /translation="MESYQIEITTAAKNYHMENYENLIKAIILTESKGKGTDLMQSSE
                     SLHGEAEIIDNPADSIDQGVKYLAEMIVEAKKQGCDLWTAVQAYNFGKDYIAYVKNHG
                     GENTVELAEAYSKDVLSPLLGNEEGKQYRYWRIQSLLYNGGYLYHNGGNMFYPDIVKM
                     NEKIIETYEKVFK"
     gene            12623..13483
                     /locus_tag="ONT85_001786"
     CDS             12623..13483
                     /locus_tag="ONT85_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HCR2378755.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            13668..15983
                     /locus_tag="ONT85_001787"
     CDS             13668..15983
                     /locus_tag="ONT85_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HCR2378756.1"
                     /translation="MNGLTQQEVVQKMASGQQNDYQEDASKSTKQIFSDNILTLFNFL
                     NFGIGICLLFVGAYSNMAYLAIIVVNIVIGIYQEIHARNLVRKLSIVSQSEVLVIRDG
                     KEQSISTKELVLGDLVKIGAGEQIPADLQVISGRAEANEALLTGESDLIVKETGAELL
                     SGSFLTSGSVMAEVIRVGADNYAVRLTSEAKTHKAIRSELVDSIRKVSKFTSFVIIPL
                     GIILFLEALFIRDASIKISVIASAAALLGMLPKGLVLLISIALTTGVTKLAKKRILVQ
                     DMYSIETLAHVDTLCLDKTGTITEGKMSIQRVEALHPLYEKAIPEIMGTYLAESSDNN
                     ITMRAMRDYFELSNRYDVTNKVAFSSDRKWGAMEFPELGVVYVGAPERLVEEDRLPDS
                     LKTAQANGYRVLMLGIAPNQQLNEEKPAFVEPVALFEIDDPIRKNAQETLAYLHEEGV
                     DLKVISGDNPFTVSNIARRAGLPNYDAYVDLSQITEEMEVREAAHRYTVFGRVSPQQK
                     KLLVNELKESGRTVAMTGDGVNDVLALREADCSIAMAEGDGATRQIANLVLLDSDFTT
                     LPEVLFEGRRVVNNVTKVSGIFFIKTIYSFILSIICAVTAIGFPFIPIQITLIDLAIE
                     GYPSFFLSFEQDKRKITYRYLPTALINALPNALLVVVNIIAVYLLGKTFAWGNAETTT
                     LMYYLLIGISCMAVVKACYPFNPLRIFLAVTTIVGIYVAAMLFHGILEVNLGLGESVG
                     YFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            16165..18006
                     /locus_tag="ONT85_001788"
     CDS             16165..18006
                     /locus_tag="ONT85_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HCR2378757.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            18030..19139
                     /gene="rpoD"
                     /locus_tag="ONT85_001789"
     CDS             18030..19139
                     /gene="rpoD"
                     /locus_tag="ONT85_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HCR2378758.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            19359..21458
                     /locus_tag="ONT85_001790"
     CDS             19359..21458
                     /locus_tag="ONT85_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HCR2378759.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            21654..22517
                     /locus_tag="ONT85_001791"
     CDS             21654..22517
                     /locus_tag="ONT85_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCR2378760.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            22622..>23076
                     /locus_tag="ONT85_001792"
     CDS             22622..>23076
                     /locus_tag="ONT85_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HCR2378761.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKE"
BASE COUNT         7770 a         4038 c         4810 g         6458 t
ORIGIN      
        1 tttaaggatt gttaaaattt aatcttagaa aaaaataact ggaacaagag ctatttgttt
       61 ttacagcaaa gaatggtata tttttgtagg aggtggctta cttgaaagaa gtcgttatga
      121 tagatgctgc aagaacgccg ataggaaagt atcgcggaag tttaagtcca tttactgcag
      181 ttgaattagg cacacttgtg actaaaggat tattagataa aacaaaactg aaaaaagata
      241 aaatcgatca agtaatattt ggaaatgtcc tgcaagctgg taatggtcaa aatgttgcca
      301 gacagattgc tttgaacagc ggtctccctg tagatgtccc agcaatgacg atcaatgaag
      361 tttgcggatc tggtatgaaa gcagtgatct tagcacgcca gctgatccaa ttaggtgaag
      421 cagaacttgt gatagccggc ggaacagaga gtatgtctca agcacctatg ctgaaacagt
      481 atcagtcaga aacaaatgaa tatggtgaac caatttccag tatggtcaac gacggattga
      541 ctgacgcatt ttcaaatgca catatgggat taaccgcaga gaatgttgca acacaatttt
      601 ctgtgagcag agaagagcag gatcgctatg ccttgtcgtc ccagttgaaa gcagcacatg
      661 ctgtcgaagc cggtgtattt tctgaggaga tcatcccagt caagatttct gatgaagacg
      721 tgttatctga ggatgaagca gttcgtggaa atagtacatt ggaaaaactg ggcacgttac
      781 gtacagtatt ctcagaagaa ggaactgtaa cagcaggaaa tgcttccccg ttgaatgacg
      841 gtgcctctgt ggtgatcctt gcatccaaag aatacgcaga aaataataat ctgccttatt
      901 tagcaaccat caaagaagta gcggaggtcg gtattgaccc aagtatcatg ggaatcgctc
      961 caatcaaagc cattcaaaaa ttgacagata gatcaggaat gaatttatca acaatcgatc
     1021 tttttgagat caatgaagca tttgcagctt cttctatcgt agttagccaa gaactgcagt
     1081 tagatgaaga aaaggtcaat atatatggag gagcaatcgc attaggccat ccaattggtg
     1141 caagcggtgc acgtatcttg actacattag cttacggatt gcttcgtgaa cagaagagat
     1201 acggtattgc ttcattatgt attggtggag gtttaggctt ggcagtcttg ttagaagcga
     1261 atatggagca aacacataag gatgtgcaaa aaaaaaagtt ttatcagctt acgccatctg
     1321 aacggcgcag tcaattaata gaaaaaaatg tattgacaca agaaactgcg ttgatttttc
     1381 aggaacaaac actttctgaa gagctctccg atcacatgat cgaaaaccaa gtgagtgaag
     1441 tggaaatccc aatggggatc gcgcagaatt ttcaaatcaa tggcaagaaa aaatggattc
     1501 cgatggcaac agaggaacca tctgtcattg cagctgcaag taatggtgca aaaatctgtg
     1561 gaaatatctg cgcagaaaca cctcagcggc tgatgcgagg gcaaatcgtt ctatcaggaa
     1621 aatcggagta tcaagcagtg atcaatgcag ttaatcatcg aaaagaagag ctgattcttt
     1681 gtgcaaatga aagttatcct tcaatcgtga aacgcggggg cggtgttcaa gatatctcta
     1741 ctcgggaatt catggggagt ttccatgcat acttatctat tgatttttta gtggatgtaa
     1801 aagatgcaat gggcgctaac atgatcaact cgattcttga gagtgtggca aacaagttac
     1861 gggagtggtt ccctgaagaa gagattctat tcagtatttt aagtaatttt gctacagaat
     1921 cattggcttc tgcctgttgt gaaatcccat tcgaacgctt agggagaaac aaagaaatcg
     1981 gtgaacaaat tgccaaaaaa atccaacagg caggggaata tgcgaagctt gacccttacc
     2041 gtgcagcaac gcacaacaaa gggatcatga atggtataga agctgtagtg gcagctacag
     2101 gaaatgatac acgtgcggtt agcgcttcga tccacgccta cgcggcaaga aatggattat
     2161 accaaggatt aacagactgg cagatcaaag gggataaact agttggaaaa ctaacggtgc
     2221 ctttggctgt tgccacagta ggcggagctt ccaatatcct gccaaaagca aaagcatctt
     2281 tagctatgtt agatattgat tcagccaagg aactagcaca agtaattgct gcagtagggt
     2341 tagctcaaaa cctggctgct ttgcgtgcat tagttacaga aggaatccaa aaaggccaca
     2401 tgggacttca agctcgttca ttagcgatct caattggggc tattggtgag gaaatcgagc
     2461 aagttgcgaa aaaattacgt gaagcagaaa agatgaacca gcagacagct atacaaatat
     2521 tggaaaaaat ccgggaaaaa taaaaaagca atatctttag aagaataaag caagatttac
     2581 agttatttta tttactgcag atcttgcttt tttctctatc agaaaatatg tttattatta
     2641 gaatttttta tagttaaggt taggcatctt aagaaatcag gcaactccaa gatatcttca
     2701 ggtagaagag aagtcgccta acattgtagg atcataggtg atttgttgga caggtcaagc
     2761 tgatatttga gcctgatgtt agaaggaatt tctttttact tgctgtagaa attattatat
     2821 gactaaaaag gctgggattt taaatcccag ccttttatga caaatagaat ttttatcgat
     2881 cagtaatcgc tgtcgctgca aattgttcga tcaaaggaat caaagtcaat ttgactttgt
     2941 atttccgttc gagtgctttt tcgataacga actcattttc agtagtcata gaaatagttg
     3001 ctaatttacg aggttcatca gcattttttt cattaataac tacttcatgt tcattttgtt
     3061 ctgtgtaaaa tttaggatac tcagcaacgg cttttgaaaa atcagcaaaa cttaaagcgg
     3121 cattcgtatc agttgagcgt gtattttcag ttgtgtccgc ttttggtttt tccgtttttt
     3181 cttctggcac actgactgtt tcttctgtaa ctggtgtcgc aacagcttgc gcagttggtt
     3241 catcgttttt caatcgttga tcattccagt aagacacgat ttcaataaga agattgtcac
     3301 ggaatcgttc gaaaatcccg ttttctcctg aacgaattct ctttaataca gtattgaagt
     3361 tgtcatcgat tggcacgttg atccgtgtat cttcatttgt tttcccagct aatccgatcg
     3421 ttaagatttg acggtttttg ggatcaagca tgacttgtaa cggcaaacca taacgtcgca
     3481 agctctcttc ttctagagaa cggaataatt gtcctcttac accatccaat gaaggacgga
     3541 attgatttaa taatttttgt ttctgttttt tcttcatatg ttctctcctt cgctcatacc
     3601 ctttcgatta taagaaaaaa acagccaaat gaaaaggagt aatgcaaaaa aacttgcgta
     3661 tacgcaaatt acaacagaaa aatttgcgta ttttcccgtt ttttgaaaga aaaataaaaa
     3721 atggaacgga ttatgtatat atacataatc cgttccattc tttctatttt ttcggtatca
     3781 aggcaaaaac aggtagttca ggtgaataaa tgacttcatg ttcgtgccag ggtttgacca
     3841 ttagcgaatg atcgaaccgt tttttcagtt cttcgatgat ttgatcatgt gtccagtttc
     3901 ttgcaggaaa atgttggatt ttctctgcat cgatcaaaaa gacaaaatcg gaatgaaaag
     3961 tctgggtaac ttcttccgag agagaattca agcgttcgat tggatccata aaatcacctt
     4021 tcgacttaaa attgggtagt atctgggtct tttgctttgc cgactacacc atctaactga
     4081 tcgatttgtt tcatttcttc ttctgttaat tcaaaatcaa ataattcagt attttctttg
     4141 attctgcttg gtgtcactga cttaggaaga ggaaggaatt cgtgttgcag cgaccaacgc
     4201 aaagcaactt gtgcaatcgt cttgtcatgt gcttcagcga tttgtttcat ttcaggcaca
     4261 tcgaagattt taccagtgcc taatggacta tatgcttcga ggatcatatc atgttttttt
     4321 gcatactcaa ctacagttgt ctgtaattcg cctggtgcaa ggaaaacttg attgaccatc
     4381 ggcataattt ttgctgtttt tgctagtgta tctagatggt ggggtaagaa attgctcaca
     4441 ccgattgctt tgattttacc tgcttcatac agttcttcaa atgctcgcca tgtatctgca
     4501 tttgcttgtt cccaattatc acgaaaagca acagggtttg gccagtggat caaaaacaga
     4561 tctaaataat ctgtttggag cttcttcaat gattcttcaa aagaagacat cacaagttca
     4621 tagctgtggt ttgcattcca taattttgtt gtcaagaata tctcttctct aggaattcca
     4681 ctttccttaa tcgcttggcc gacgctttct tcattcttgt atccttgggc agtatcgata
     4741 tggcgataac ctgcagccag cgcttcttta acagaggaaa cagcgacatc cccatcaggt
     4801 gtttgccatg tgccgaatcc tactaccggg atttcgtaac catttgatag acgataagtg
     4861 cttgttaatg aattcatact ttcagctcct tttttattcg ttcaactata agtgtagcgc
     4921 atttttcgaa aaaaagaatc cattttgttt ctactcttga aatttttgtt taaaagaaaa
     4981 tgattttgat tctggtattt ttgatttttt ttccgtaaac taagacagaa gggaatgaga
     5041 aaatggatat ctatttaaaa aaagcaattc tgcatattat tgacagagaa actggttctc
     5101 cagtcttttc acaaaaagag ctggatctga caaaagaata tattcgtgat tttcttcaaa
     5161 agaagatcca aaaaatctct tctgctcaaa caaaaactgg acaactagca gaagaaagca
     5221 tgatcgttga atcacttaaa atcgctcaag aaaattttgt ggaagcaagt gaagcaatcg
     5281 ttcagcggtg gttcgatatc taccaagaaa gtgaagaagc acctagtgca gatgtatttg
     5341 ttgtcttgta tgaattagat acagtgatgt acttatcttt attgaaagtc aattatcgtg
     5401 aggcttacac tcactttgtt gaagcagacg aagcagggat cgacaaccgc ttgattttga
     5461 atcgagcaat tctaggggcg aaaacacaaa aagcagatga agcattcgct gtgaatttga
     5521 ctgattggac ctacgaatta attgaaaaaa aatatgagtt ttctggcaaa aaagagcttt
     5581 atttttctgg aagagtgatc gaaagtgttc ctgcaccttc tttagaagaa aatgtaaaag
     5641 tgatccaaaa agtagccaag catcttggaa aaaaatttga gacagaagaa tttgatatca
     5701 tggctgattt gaaagaagct gtctatgata cgatagaaga aaaaggtcgg ttggatcatg
     5761 agatgattgc ggaaaaagtc ttcaaggaaa acatcacggc aaaaatggct tttaaagaag
     5821 aagtacaaga gcaaggattt gtcccagaag cacctcctgt aaaagaagta caagaaattt
     5881 ctgagaaaaa atttggaaaa caaaaattca agctatcaaa cgggatcgaa ctgatcgtac
     5941 cagtagatgt atatcgcaac ccagatttga ttgaatttgt gaataatccg gatggtacga
     6001 tatccgtgat gatcaaaaat gtggatgaag tattgaatcg tttataaaaa tgactagaat
     6061 attaggtgtt caagtaccag tgatcagtta aatgaattgt gtcaagagaa ggatcggaaa
     6121 agacgaatat aacaaaaaaa gagcgtgaga caaaagtaaa gaacactttt gtctcacgct
     6181 ctaaacacgt ataaacggcg gaagcagaag caactccttc ttatttcttg gagttaaacg
     6241 cttctgtccc agcctctttt tatctaataa aattttaaga ccatttacta attatcgatt
     6301 ttttctgccg catttttagg cacttcagaa gcctttattt cttcccagct taatacacct
     6361 ttacgaggat tgactttgag tttaagatac gcattcatct ttaatggatg ttctctttct
     6421 tcgcgaagcg tcacctgttt tttttcacca tctttattat aagccgcttg cttgtaggta
     6481 taagtcgtga atttctctcc gttatctgct tgggtagctg acatttcgcc attcgtcgtg
     6541 atttttgtat aataagcttc gccgccgtaa aaataatgat aagctgcaaa acttccgccg
     6601 agaaatatgc ctaaaacgac aagtacgcca attaattttt tcatgtactt cacctctttc
     6661 cttattatta acgaaataga agatttcgtc tattgaaagt tgttggcaac agctgaattt
     6721 taaacttttc ctcattttgt tcttattgct tcaaaagaaa gttaaggact atcttgaagt
     6781 ttttctcaaa aaacacattt ttttatttat aaaaggtact tttttcgcgt ataagacaga
     6841 cgagtgctct tagttgactt tgaccaatat tgacgtataa taagcttagt tcgaatctta
     6901 caaaaagtaa gtcggacgta ttctttttga tttagtcaaa aaaagactat aataaaaagg
     6961 tgagagctga aatttttcag tgatctatat tatttaggag gtttgtaaat tatggcaaaa
     7021 gaacattatg acagaagtaa accacacgtt aacattggta cgattggaca cgttgaccat
     7081 gggaaaacta ctttaactgc tgcgatcact acagtattag gcaaaaaagg cctagcaaat
     7141 cctcaagact atgctagtat tgatgcagct ccagaagaac gtgaacgtgg gattacgatc
     7201 aataccgcac acgtagaata tgaaacagaa aaacgccact acgcgcatat tgatgcacca
     7261 ggacacgcgg actatgtgaa aaacatgatt acaggggcag cacaaatgga tggtgcaatc
     7321 ttagttgttt ctgcaactga cggtccgatg cctcaaacac gtgaacacat tttattgtca
     7381 cgccaagttg gtgtaaaata cctgattgtt ttcttgaaca aagttgattt agtcgatgat
     7441 gaagaattga tcgatttggt agaaatggaa gttcgcgagt tattgagcga atatggtttc
     7501 ccaggcgatg acactcctgt gatcaaaggt tccgcattaa aagcattgca aggcgatcca
     7561 gatgctgaag ctgctattat ggaattgatg gatacagtag atgaatatat cccaacacca
     7621 gaacgtgata cagataaacc attactattg ccagtggaag acgtcttctc aattacaggt
     7681 cgaggaactg ttgcctcagg tcgtattgat cgtggtgctg ttcgtgtcgg tgatgaggta
     7741 gagatcgtag ggatcaaacc tgaaacacaa aaagcagttg taacaggtgt agaaatgttc
     7801 cgtaaaacgt tagattacgg ggaagctggg gataacgtag gcgtgttgtt acgggggatc
     7861 caacgtgacg atatcgaacg tggacaagta cttgctaaac caggttccat tactccacat
     7921 acaaaattca aagcagaagt gtacgtgttg acaaaagaag aaggtggacg tcatactcca
     7981 ttcttcaaca actatcgtcc acagttctac ttccgcacaa ctgatgttac aggaacaatc
     8041 acattgccag aagatacaga aatggtcatg cctggtgaca acgtgacgat tgatgttgac
     8101 ttgatccatc caatcgctgt tgaaaacgga acgacattct caattcgtga aggcggacgg
     8161 acagtaggtt caggtatcgt tacagagatc gaagcataag aaacgcgact cgaatatttt
     8221 ggtaatgaaa aatgtttaaa ataaaagagc ggtccaaaca ggatttctgt ttggaccgct
     8281 ctttgttctt caattgtata agaagagatt gtagaagcat gaactttcgt tttacttcag
     8341 tctcttttat tcatcatgag agtgttcatc tatcggtatg cagtttttaa tactttcttc
     8401 gtatccattt tttcaaacca cttattctac cttgtgtctg ctcaactggc gcattatcaa
     8461 taatcagcaa agcaatcata acatatgcaa tccaactatc tctagaatat agagtataac
     8521 gtgagaccca gtttgtagcg tacttgcttt tatattctct taatccatgg aaagaataaa
     8581 aatgagaacc aaactcgtaa accaagtatg caatcctttc ctgtaaaaaa ctctttctgg
     8641 aagttccgac atttgaaaga ggggccatcc ctaaattgaa ccattggata tgttcttctt
     8701 tcatataggc aaacaaatgc aagaacaaga aatccatact gccagaaggt gcttttttag
     8761 aatcatatcg catcagatca atcgttccta ctttattgtt cgtgtaagta ggcatgatcg
     8821 ttgcaaaagc gacaatttct tcttgggtat ttttgacaac tgcaataggc gctctttgta
     8881 gataatcttc agagaaaaag cctaaagaaa accctttttc ctttcgtgaa cccaaccaat
     8941 tatcagagat atttttgaaa gtactcattt gctcagtaga aaatggtggt tgtaagacat
     9001 caaatgtaaa tccttctcgt tcgatccgat tcaagacagc acgagtgccc ttcatttttt
     9061 tccctgaagt tgtaaaacta ttcaggtcta cataagcttc ttcccccatc ttaatgaaat
     9121 cgtagccgaa ttcatgcaaa atcataacaa tctcttcgct tgtttcgtag aaaactggta
     9181 aatagcatag tcgatctgtt tcttcgataa aagcttcgat tgcttctgga aaatcttctt
     9241 tcttaccaga aggatcaccc attacaatac atttattatt gaagcaagcg aattggagaa
     9301 gaacagttgg ttcaccgtct ttttcatagg caaacatccg cttatctttt aagaagatca
     9361 attgactatc ggagtttcct ccataagttg taaggatctt cagggccttt tcttcattaa
     9421 aggcttcgcc aatctgtttc ttttctcctt gaaggaaatg aacaaataga acgatcatga
     9481 aactaacagc gatgatggcc aacaatcccg aaaaccaaat cttttcagaa ggaaataaga
     9541 aaaaggaaat aaaatggtga cgccgatgag ggaaatccgg cagattgtat accccaataa
     9601 cgatgtaaag caaggacagt gcaccaataa tgattccatc gatcgtacgc cactcccatg
     9661 aatagatcaa ttgttcccga aataattcgt ttttagaagc aagaatgatc agaagcagaa
     9721 tagacaagaa aaccaccggt gtgaaactaa aatcacttaa taaaacataa agtaaagcta
     9781 gaacaatcaa gaagattgta ggcaaatatg ctcgtttcac acgtgctgcg atccctctgc
     9841 ccataatgat taaaagaaat cccaatagta tactaggaaa ttggataata aaatgaaatt
     9901 tcaatgggtt caaactatga agccagcgaa actctgtaaa agcttgcggg atagtggcag
     9961 aaagtactag catgattcca gaaaagtata ataagacaac cacgatttta tgcgcaattt
    10021 ctgttgctaa ttgtttaggg ataccagaat aacgctgatc aaaagtggaa ccgatatttt
    10081 tgaagaaaaa gacgattcca atcaaaaacg gaatgatgta gtagaacagg cgatagagca
    10141 agatccacac aacgactatt tctcttggca caccaatagc ggacaacccg ataatcatca
    10201 ttacatcgaa actcccaatt tctcctggaa tcatagacac gattcctatc actgaagcag
    10261 caatgaataa tggaattgtt tgccacagat caatccggat ttccattaaa taaccaacag
    10321 aaaggaaact aaacaggaca ccggtccatt ccaacaatga aactagcaat agctgtcctt
    10381 gagttttaag tgaggtacca ccaaggaatc ctttcttttt gaaagtagta aataaaaaga
    10441 cgacaggaaa atatagaccg ccgccgatca accatatcca atattgcttc aaaaaaggag
    10501 caactggcgt gaagactaca agtaaaaaag aaagcagact gtaaatagaa agtccagcca
    10561 tcaaaaacaa gaaaactttc gataatgctt ggatcacgtt tctgctctct ttcttttgtc
    10621 catatagttc agagcgaagc cccacactaa caaacccgcc gaaaccggca atattgttga
    10681 tcgtattgat cagccagctt gtctcaaaca ggtaacgagg attctgcttt tgttcaagca
    10741 tccgattcaa aataatatca taaccaacca taggtaacac agagataaga ccaatcaata
    10801 gcattagtcc gcttttccat acaggtatag tggcaaacgt ttctcctaat tgctggatag
    10861 atagagtttt accgatggac atcaattcac cgacaataat gacaatgaca gaaatcaaaa
    10921 agattgtttt gaacagtcct aggtggtttt tcagccattg tatggtattt tttaacaaat
    10981 aaatcacctt ctattcctaa tagttatgtt tcagaaagaa attgaagaac ttctcgattg
    11041 aagcgttcag gatcttttct agctaattca tgtccttgtt ctttcacaag cacaaaggaa
    11101 gcttttggta tagttttagc tatatacaaa gaatgtttta gcttgattac atcttttttt
    11161 cccacgatga tcaaagtggg ggagttgatt ttttttaaat cattttctgt aagcccgatg
    11221 tcatgcagca aaagtttgat aactaaaaga tttttgcgaa gacttgggcg aaataaagag
    11281 agtaaccata cccatgcata atgaatatta ctgatgactc tggcagaaaa acgaacgcct
    11341 ttaacaagcg tgtttccaga atttaaaatc aatcgatgaa cttttttagg gaagctgctg
    11401 gcaaatacca gtgctagatt tgctccatca ctaaatccaa gaaaatctgc ctgatcgatt
    11461 ttttccagca acatgatcgt attcagatcc tcggccatta actgaaaatt caacatagat
    11521 gcttcgtttg tcgaacgacc atgtcctcgg ctatctacta gatacacggt atagtagcgt
    11581 tcgagtacag ggacctgttc tgaaaaaaag cggccgctgc cgtcattgcc atgcagtaaa
    11641 aagagaggga agccttgacc gctcttttca tagtaaattt cgctcccatc tggcattttt
    11701 gcatatttct tttctttttt cacataatca cctactgtgt aaagtataac gattatatca
    11761 gtcattgggc aattgttttt actgaaaaac aacgatcatt ttcgtaagat gtcaaaaaat
    11821 acaggaaaat tcattttttc ttaaggcgta cacagaaatc aaggaagaaa tagccaagaa
    11881 gataaagaaa tagtaagata aaaaaagatg tcaaaataac taggtgatga aatgaagcga
    11941 aaacgattat ctattatccg tattcttttt tacatgatca ttctgacggt tattggtgcg
    12001 gtgttgtttt tatgaggaag aacttatcgt tcggtaaaac gagtggaaag ctatcaaata
    12061 gaaataacaa cagctgcaaa aaattatcat atggaaaact acgagaattt gatcaaggcg
    12121 ataatattga ccgaatcaaa aggaaaaggg acagatctca tgcagagctc ggaaagtctg
    12181 catggagaag ctgagatcat tgataatcca gctgacagta ttgatcaagg tgtcaaatat
    12241 ttggcggaaa tgatagtaga agcaaagaaa caaggatgcg atttatggac agctgtccaa
    12301 gcatataatt ttgggaaaga ttatattgcc tatgtaaaaa atcatggcgg agaaaacaca
    12361 gtagaattag cagaagcgta ttctaaagat gtgctgagcc cacttttagg aaatgaggaa
    12421 gggaaacaat accgatattg gcggatccag tccttgcttt acaatggcgg ctatctctat
    12481 cataatggag gtaacatgtt ttatcctgat attgtcaaaa tgaatgaaaa aataatagag
    12541 acctatgaaa aagtctttaa gtaaaatagc aggaaatgtt caatcatgaa taaaattaaa
    12601 tgaaagaagg agtgcgcaaa tagtggaaaa aaggcaaaaa atggaaaatc aagaagtggg
    12661 gtacacaacc aaagactgga tgttacggtt tgtgaaggga atgtttatcg gttcaggatt
    12721 tatcttgcca ggagtcagcg gtggtgcatt agcagctgtc tttggcatgt atgagcggct
    12781 gatttctttt ttagcccacc ttacaaaaaa tttcaaagaa aatgttttgt tttttctgcc
    12841 tgttggtcta ggcggagtga caggcatctt tctactttct tttttagtca gttttttatt
    12901 aggtgcgtat gaaacaacga tattatggtt ttttgtcgga tgtatcgctg gaactgttcc
    12961 tgcgttatgg aaagaagcag ggaaaaaagg aagagatact tctgatgtga tcatcatggt
    13021 tgttactttt gttctggctt atttgttttt attatacggc gctcaattat ttgacgggca
    13081 agtcgatcag aattttggaa catggctgat tgccggcgga ttgattggtt tgggtatgat
    13141 cgtcccagga ttgagccctt ctaatttttt ggtgtatatg ggattgtata aagcgatggc
    13201 tgacggattt aaagaagtac gacttgaagt gattgtgccg atcggaattg gtgggattct
    13261 ttgtgttctt ggattgtcaa aaatcatgga ttacattttc tcaaaggcat atagcaagct
    13321 attccatttc attttaggtg tagtttttgc ttcaacgatt atgatcattc ctaccgatta
    13381 cgcgggattg ggagttgctg gggtactagc ttgtcctgtt ctttttatag ccggtacagc
    13441 actaggttgg tggatgagcc gtttggagga acaatataag taaagataga ttgataataa
    13501 aaacagcaca cgggtgattc aaacaaaaag gttggcatct tttttgtaaa atcattcgtg
    13561 tgtttttcta taaattttat aaaaacaagt tgtttttaat cttggaacac gctagactta
    13621 aacacgtgat tatttttgaa agttggcgga aaatggagaa aaagaaaatg aatggactta
    13681 cacagcaaga ggtagttcag aaaatggctt ctggccagca aaatgactac caggaagatg
    13741 catcaaaaag tacaaaacaa atattcagcg ataatatatt gacgttattc aattttctga
    13801 actttggtat tgggatttgt ctgttatttg taggtgcgta ttccaacatg gcgtatttag
    13861 caattattgt cgtcaatatc gttattggaa tttatcaaga aatacatgca agaaatcttg
    13921 tacggaaatt atcgattgtt tcccaatcag aagtactagt gatccgggac ggtaaagaac
    13981 aatcgatttc tactaaagag ctggtattag gagatttggt aaaaatcgga gcaggagaac
    14041 agataccagc cgatttacag gttatatctg gacgggcaga agcaaatgaa gctcttttga
    14101 caggcgaatc tgatttgatt gtcaaagaaa ctggagctga attgctttct ggaagctttt
    14161 tgacaagcgg ttctgtcatg gctgaagtta tccgagtagg tgcagataat tatgctgtcc
    14221 gcttgactag cgaggcaaag actcacaaag ctattcgttc tgaacttgta gattcgattc
    14281 gcaaagtatc caaattcact agtttcgtga ttattccttt ggggatcata ctatttttgg
    14341 aagcactgtt catccgcgat gcttctatca aaatttctgt tattgcatct gcagcagctt
    14401 tgttaggcat gcttccaaag ggtttggtat tgctaataag tattgcatta acgacagggg
    14461 taaccaaact ggcaaaaaaa cgaatccttg ttcaagacat gtattcaatt gaaacgctgg
    14521 cccatgtcga tacattatgc ttggataaaa cagggaccat tactgaaggg aaaatgagta
    14581 tccagcgggt agaagcacta catccgctgt acgagaaagc catcccagaa attatgggga
    14641 cgtatctggc agaaagcagt gataacaaca tcactatgcg ggctatgaga gattattttg
    14701 agctttctaa tcggtatgat gtgacaaaca aagtcgcatt ttcttctgat cgcaaatggg
    14761 gcgcgatgga atttccagaa ctgggagtcg tgtacgtagg agcgcctgaa cgtttggtgg
    14821 aagaagatcg gcttccggac agtctaaaaa cagcccaagc gaatggctat cgcgtactta
    14881 tgttggggat cgctccaaat cagcaattga acgaagaaaa accagcattt gttgagccgg
    14941 ttgctctgtt tgaaatcgat gatccaatcc gaaaaaacgc tcaagaaaca ctagcttatc
    15001 ttcatgagga aggtgtggat ctgaaagtga tttctggaga caatcctttt actgtctcca
    15061 atatcgctag acgtgcagga ttaccgaatt atgatgcgta tgtcgatctt tctcaaatta
    15121 ctgaagaaat ggaagtgcga gaagctgctc atcgctatac ggtttttgga cgagtgagtc
    15181 cgcagcaaaa gaaattgctg gtaaatgaat tgaaagaaag cggcagaacg gttgcaatga
    15241 caggcgacgg tgtgaatgat gttttggcgt taagagaagc agattgcagc attgctatgg
    15301 cagaaggtga tggagcaaca cgtcagattg cgaatctggt attgttggac tctgatttta
    15361 ctacattgcc agaagtcctg tttgaaggac gccgagtggt caacaatgtg acgaaagtct
    15421 caggcatctt ttttatcaaa acgatttatt catttatttt atcgattata tgtgccgtta
    15481 cagcaattgg atttccgttt atccctatcc aaatcacgct gattgattta gcgattgaag
    15541 ggtatccttc cttcttttta tcttttgaac aagataaaag aaaaatcact taccgttatt
    15601 tgcctacagc acttattaat gctttaccaa atgccttgct tgtagtagtg aatatcattg
    15661 cggtgtatct acttggaaaa acattcgcat ggggaaatgc tgaaactact actttgatgt
    15721 attacttgct gattgggatc agttgcatgg cagttgtaaa agcttgttat cccttcaacc
    15781 cattgcgcat cttcctagca gtaacgacga tcgtagggat atatgtagct gcaatgctgt
    15841 tccatggaat tttggaagta aacttagggt tgggagagag cgtaggatac ttctttgtat
    15901 tcatgctcgt aaatattgtt cttcgagtat tgtactggaa ggctgatatt caaaatcagt
    15961 tactgaaaag agtagcaaat tgatgcttaa tgagatagaa atgtgttata cttgaaaaag
    16021 caatgaaata tgatacaatc aaacatgtat aattgtgtcc tttaaaatcg cactgatttc
    16081 agttagtgcg attttttacc ggttattctc ctgaaatatc ttctgttaaa cccaaaagat
    16141 atcaatagag cagggggtga aaaaatggct caacggattc ctcaagaggt tatcgaaaca
    16201 gtacgcagtc aaacgaatat cgttgaggtg attggacaat atgtccagtt gaaaaagtct
    16261 ggtaaaaatt acttggggct gtgtcctttt catgaagaac gtacaccgtc tttttctgtt
    16321 gcagaagaca agcagatttt tcattgcttt ggttgtggta aaggcggaaa cgtgtttact
    16381 tttcttcaag aactagaagg tctaagtttt ccagaagcag tggtaaaagt ggcagacttc
    16441 gaacagatac cgttagaaga tcaatggcgt cagcgtacta tagctgtcca aaatcctgaa
    16501 tctgctacgg ggaaactgat tgctgctcat gaaaaagcag cggaaatcta tcatcacatg
    16561 ctattgcata caaaagctgg acagccagct ttagaatatt tgcaaagtcg tgggttgtct
    16621 ttagaattaa ttgaagaatt caacataggt tttgcaccta atgagcgttc ttttcttcag
    16681 caagtattca agaatgaatc aatttcaaat gaattgttag aacgctctgg attgtttgtc
    16741 acgcatgatg atggccgtat gtctgatcgt ttttatcaaa gagtgatgtt tccacttcgt
    16801 aattctcaag gccagacgat cggtttttca ggacgctggc ttgcacctga aaaagggcag
    16861 gaaaaagatc agcccaaata cttgaatagt ccagaaacag aactgttcaa taaaagacaa
    16921 gttttattta atcttgataa agcaagaagc gatatccgga aaaacggaga aattttgttg
    16981 ttcgaagggt ttatggatgt gattgctgca tggcaggctg gtatcaaaac tggattagct
    17041 tctatgggga caagtctaac agctcaacag atccaacaaa tggaaaagct gacgaaagaa
    17101 ttggtctttt gctatgacgg agacaacgca ggttttgaag caaccaatcg agggattgaa
    17161 ttgcttcggc aaaacagtcg attagcactt tctgtcgtag tcgttccaga aaagcttgat
    17221 cctgatgaat atctccgtaa gtatggagaa gcatctttcc aagaattagt ggctcatggg
    17281 cgcgaaacag tctttacttt taaatcacgt tatttaaaac aagggaaaaa tttagataat
    17341 gaaaaagaca agatcgaata cctcgaagaa ttactagttg aacttagccg agttgtttca
    17401 ccaatcgaac aagatatgta tctatctcaa ctgtcaacag agtttcagat cggcagagag
    17461 acgatacaaa aacaactgcg cgatcttcga aggacaaatc agcaaaatcg tcaagaacct
    17521 caggaaagtc aacacactca ggttacgaga caagaaactg cacggaaaaa gagacctttg
    17581 acgcaagtag aaaaggcaga gcagattcta ttatatcgtg catttaaaga agtttctgtc
    17641 cgtaatattt taaaagaacg agaatttcag tttattcatg atgcatatgc agaagtttat
    17701 tttttattcg atgcatatgt ctcacaacat tatgaattca accttgcaga gtttttagac
    17761 tttttgcaag atgaaaattt aaggcggttg gtggtggaaa tcgaatattt accggtggca
    17821 gaagaaagtt cgccacgaga aatccaagac ttgctagatg tgatcagcaa atctggctta
    17881 gctgatgaaa taacattaaa aaaacaaatg cagcaagaag ccagacgaac tggaaacaaa
    17941 cagcttgagc ttgaattgac gattgaaatc atcaatttaa cgaaacagtt aaaacaagcc
    18001 aaatagctac tcaagggggg ccttctttta tggcaaatgg aacagataaa aaaacgtatg
    18061 aagcagcagt agcagctttc atcaaagaaa acaaacccaa acgacaagtt gtatatgatg
    18121 atttatccaa tcagcttgct acaccattca ctttgaatgc agatgaaatg gacaaattga
    18181 tccaaaagat tgaagacgca ggtatcagtg tggtggatga aaatggtgat ccatcaattc
    18241 acagcttgaa gagtgctgag aaaaaagctg aacaagcaaa aacagaagat ctttctgcgc
    18301 caaccggtgt aaaaatcaat gacccggttc gtatgtatct gaaagaaatt ggccgtgtct
    18361 cccttttaac tgcggaagaa gaagtagcgt tagcattgaa aatcgaagaa ggcgaccaag
    18421 aagcgaaaca gcgtctggca gaagcaaacc ttcgtttagt agtaagtatc gctaaacgtt
    18481 atgtaggacg aggcatgcag ttccttgact tgatccaaga aggaaacatg ggactgatga
    18541 aggcagttga aaaatttgac taccgcaaag gatttaagtt ttcaacctat gctacatggt
    18601 ggattcgtca agccatcact cgagcaatcg ctgaccaagc acgtacgatc cgtattccag
    18661 ttcatatggt tgaaacaatt aataaattga tacgtatcca acgtcaattg ctgcaagacc
    18721 ttggaagaga acctacgcca gaagaaatcg gtgcggaaat ggatcttcca acagaaaaag
    18781 tccgagaaat cttgaaaatc gcgcaagaac ctgtttccct tgaaacgcca atcggtgaag
    18841 aagatgactc tcacttaggt gatttcatag aagaccagga agcaacaagt ccagcagaac
    18901 acgcagctta tgagttacta aaagaacaat tggaagatgt actagataca ttgactgatc
    18961 gggaagagaa tgtccttcgc ttacgttttg gtttagatga tggccggacg agaacattgg
    19021 aagaagtagg gaaagtattt ggtgttactc gggaacgtat tcgtcagatc gaggctaaag
    19081 cattaagaaa attgcgccat ccttctcgtt caaaacaatt aaaagatttc ttagaataag
    19141 cgtggactat tgaccagaag agaaatcagt aaaactgttt ggtttatttt aaaagaaaaa
    19201 aagcttgaga ctacgcagtc tcaagctttt ttttctatag gaaggaaatt cattcattat
    19261 ttttaagtaa atcctaaaaa taagctattg gcgtgacgta tagaatatga tagattaaat
    19321 ggtagcgtaa acctagtaaa ggagccgatc gctaagtgat gaaaaaatgt ccaaattgcg
    19381 gatcgaaaaa aatggagcag aatactatct gtccagactg cggtcatcaa ataactgcag
    19441 aagatcagca aataaaagaa tcaattgaag aaacaaatac agatgcagct atgcaggata
    19501 aatcaactga agatgcagtc tttcctgata cagaattaaa cgatccaatc gaatggtcgg
    19561 aattgaaaga tttacctcta gaatctgtaa tggaattatt cgaaaaagca gaaccagaag
    19621 acgaagaacc acctgctcac gatcaagtaa ctaatataga gaaaaatgaa cagaagaaag
    19681 caactgttca tgagacttcg gaagaatcag gaaagaatca agaagaaacg cttgaatcaa
    19741 aacaaaaaag aagagtagct gaactgaaag aaatcgtcga taatgaggaa gaaaattcga
    19801 tactatccgc ttatatcaaa gcacatcgtg aagatacaaa agaagagcac gcgaaagagc
    19861 tgctcaaaat gatcagtgaa aaaattgcac aggaaaacaa agtagagcca gaaaaggaac
    19921 aagaaactga aaaagactct gatagagaaa acgccacagg taaagaaaca gtaaaaataa
    19981 aagacgtaaa aacggatatc acagaaaaaa ctgaagtatt tactgaaaaa caattagaaa
    20041 aggaaaaaac gaataaactg ccggaagcag agaaaaaaga aatactcgaa aacacagaca
    20101 aaaagtcaac agagcccgat gaaataaata gtgaatccga tgctgaaaat caatctgtaa
    20161 gcaaagaggg tactgatctt gaagcaaaaa tagaaaaaga agagaaccag cctaagctgt
    20221 ttgaagcaga agcgaaatca ataacaatcg tgccagatac tgaaaaaaca catcaagaat
    20281 caaacttaga aaaaacagat ccagataaag cgcaaaagaa aaaatcaaaa aaagtacctt
    20341 atctcgtttt agcagcagta cttctactcg gtggaggaag ctgggcttat tatgaccatc
    20401 atcaaaaagt agaagccgaa gtagcagcag aagcaaaaag acagcaacaa atggaagaac
    20461 ttcatgagaa acttgctgcc ttttatacag atgatcacca tcaatttatt cgtgcagata
    20521 tgattcatca agatttatca aaactaaaaa atgatttatc cagtcttaag aatgaaaaag
    20581 gatattcaga gttagaaaca acttaccaag atatccaatc taagatccga gatatccaaa
    20641 aagtgaatga gtggttcata gcacctgcca tcgtcgatga tcatctagca gatgacatca
    20701 agttgaaagc agataaagag atccaagcaa tcgaaacgga taacacagat tttggaaaat
    20761 tgattcaaca agcacaaaca gaagctaaaa atcaatatgc tgaattgcaa acagctaaag
    20821 aaaaaacagc tatagtcttt aaagacggaa aagtagtgga ttcagcaaca cgcgaccaat
    20881 acaagacggc aaaagaagca gtagaaaaag tgaaaaatac agatctatct gctcagttga
    20941 atgaacaatt gaaacaagta gataagatgc tcactgataa agagaaaaaa gaagaagagg
    21001 aaaagaaaaa agcagaagaa gcgaaaaaag cagctgaaga acaagcaaaa caagccgctc
    21061 aagcagcagc tgcagctcaa gccgctcaac aagctgcggt cgcacaagca gcacagcaag
    21121 aagcggcatc ctcgacccct tcctcttcaa actcagcgaa tcagccaatc aaatctgcca
    21181 gagcaagtga tattgccgat gcatctaatc cagcatggac gtgggcacca ggtgtccaaa
    21241 gcaaagtgat tgctacatgt atccaacgtg gatatatcgt tgaaggaggt tacagattag
    21301 aaaaagccag aatcgaaaat ggggaaggct attataatct atatgccact tcaacaaaat
    21361 ctgcattgat gaaaggcatt ggagaaagtg ctttgccatt ttacatcgta accatcaact
    21421 gtaaaactgg atggttcgga ggaaatggga gcaattaatt ttaggcaaat gaaagaggct
    21481 gtgagcctgc cgtttaacac cgtttattcg aaaaagggga gtatgacaca actgttgtca
    21541 tactcctttt ttcacctttc caaaccaagg ctcagctatt caagtagata acattatgtt
    21601 atgatagcaa agaaatgtat taagaaaacc tttcactata gagggagaaa aaaatgaacg
    21661 atttattagc aactatattt acaggtatta ttattgatga aaatgaaaaa aactactttg
    21721 tccaaaaaaa cggacaaacc tttggcttaa gcaaatcaga aggtgaacac aaaatcggtg
    21781 aagcagttga aggattcggt taccaaaatc aaaaacatga aaatcggatc actacacaaa
    21841 ttcctaaaag ccgtatcggt cattacgctt ttggaatcgt tactgctaca cgaaaagatt
    21901 taggtgcgtt tgtcgatatc ggtcttccag ataaagactt tgtcgtatct cttgatgaat
    21961 tgccgacaat gacagagcta tggcctaaaa aaggggatcg cttgatgatt tcccttagag
    22021 ttgatgcaaa agaccgtatt tggggcagtt tagcagatga aaaaatattc aaatctttaa
    22081 gcaaatatgg aacagaagaa atgaaaaaca aagatgtgat aggaacagca taccgcttga
    22141 aattaacagg tacgtacgtt ttaacggaag atttctatct tgggtttatc catccttctg
    22201 aacgatataa tgaacctcgt ctaggcgagc agataaaagg tcgagtgatt ggtgtccgac
    22261 cagacggtgt actgaatctt tcattgaagc caagagcata tgaagctata tcagatgatg
    22321 cacaaatgat tttaaccatt ttagaacgtt ctgcagatca gcaaatcaac tttacagata
    22381 aatcggatcc agaagaaatc atgaaagctt ttggtatcag caaaggagca tttaagcgcg
    22441 cattaggtaa tcttttgaaa caaggattga ttacacaaga aaacggtgtg acaaaactag
    22501 tcaaaaaatc taattgagaa tcccttgcat gtttttctta actagcttat aataatttta
    22561 attaacatag tgttatatct ttttttattt gtaattatta taaattgttg gggttgagta
    22621 aatggtcatg aatgctattt cggcgatgaa aaaaacaaaa cagcagctac atgaagcggg
    22681 gtttaagctg acaccgcaaa gagaagctac tctgcttgtg cttttggaaa atgagaaaga
    22741 gcatttatct gcagaagaaa tcttcttttt agtgaaaaaa aagagtccgg aaattggact
    22801 tgctactgtt tatcgaaccc ttgaaatctt gacagaattg aaaattgtcg ataaagtaag
    22861 ttttaatgat ggattagccc gttatgattt gagaaaagaa ggcgccaaac atttccatca
    22921 tcatttacta tgcttgaact gcggcacgat cgaggaagta gaagaggatc ttttacttga
    22981 agtggagcaa gtagtagaag aacgttatca ttttttagta aaagatcatc ggctaacgtt
    23041 ccatggaatc tgtcaggaat gctatgaaaa agaaaa
//
LOCUS       DAJSBL010000037        23065 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.037, whole genome shotgun sequence.
ACCESSION   DAJSBL010000037 DAJSBL010000000
VERSION     DAJSBL010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23065
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.037"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(109..564)
                     /locus_tag="ONT85_001793"
     CDS             complement(109..564)
                     /locus_tag="ONT85_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2378762.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(718..1926)
                     /locus_tag="ONT85_001794"
     CDS             complement(718..1926)
                     /locus_tag="ONT85_001794"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HCR2378763.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNEGAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(2038..2370)
                     /locus_tag="ONT85_001795"
     CDS             complement(2038..2370)
                     /locus_tag="ONT85_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HCR2378764.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            2518..3393
                     /locus_tag="ONT85_001796"
     CDS             2518..3393
                     /locus_tag="ONT85_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HCR2378765.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSGELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(3475..4056)
                     /locus_tag="ONT85_001797"
     CDS             complement(3475..4056)
                     /locus_tag="ONT85_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpiB family protein"
                     /protein_id="HCR2378766.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(4062..5321)
                     /locus_tag="ONT85_001798"
     CDS             complement(4062..5321)
                     /locus_tag="ONT85_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HCR2378767.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(5323..6369)
                     /locus_tag="ONT85_001799"
     CDS             complement(5323..6369)
                     /locus_tag="ONT85_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2378768.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(6604..6879)
                     /locus_tag="ONT85_001800"
     CDS             complement(6604..6879)
                     /locus_tag="ONT85_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HCR2378769.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(7122..8432)
                     /gene="der"
                     /locus_tag="ONT85_001801"
     CDS             complement(7122..8432)
                     /gene="der"
                     /locus_tag="ONT85_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HCR2378770.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(8614..9843)
                     /gene="rpsA"
                     /locus_tag="ONT85_001802"
     CDS             complement(8614..9843)
                     /gene="rpsA"
                     /locus_tag="ONT85_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HCR2378771.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(9978..10658)
                     /locus_tag="ONT85_001803"
     CDS             complement(9978..10658)
                     /locus_tag="ONT85_001803"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HCR2378772.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(10707..11342)
                     /locus_tag="ONT85_001804"
     CDS             complement(10707..11342)
                     /locus_tag="ONT85_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HCR2378773.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(11408..12811)
                     /locus_tag="ONT85_001805"
     CDS             complement(11408..12811)
                     /locus_tag="ONT85_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HCR2378774.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATKQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWETILLNLFKKNMEH"
     gene            complement(12798..13829)
                     /locus_tag="ONT85_001806"
     CDS             complement(12798..13829)
                     /locus_tag="ONT85_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR2378775.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESEVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            13860..14081
                     /locus_tag="ONT85_001807"
     CDS             13860..14081
                     /locus_tag="ONT85_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HCR2378776.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(14246..14983)
                     /locus_tag="ONT85_001808"
     CDS             complement(14246..14983)
                     /locus_tag="ONT85_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HCR2378777.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(15136..16476)
                     /locus_tag="ONT85_001809"
     CDS             complement(15136..16476)
                     /locus_tag="ONT85_001809"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HCR2378778.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(16713..18236)
                     /locus_tag="ONT85_001810"
     CDS             complement(16713..18236)
                     /locus_tag="ONT85_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HCR2378779.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(18614..19225)
                     /locus_tag="ONT85_001811"
     CDS             complement(18614..19225)
                     /locus_tag="ONT85_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HCR2378780.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(19348..19473)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(19555..20271)
                     /locus_tag="ONT85_001812"
     CDS             complement(19555..20271)
                     /locus_tag="ONT85_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HCR2378781.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(20277..20846)
                     /gene="scpB"
                     /locus_tag="ONT85_001813"
     CDS             complement(20277..20846)
                     /gene="scpB"
                     /locus_tag="ONT85_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HCR2378782.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(20830..21627)
                     /locus_tag="ONT85_001814"
     CDS             complement(20830..21627)
                     /locus_tag="ONT85_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HCR2378783.1"
                     /translation="MASKEKEERVNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDI
                     PIATITEQYMNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFGTDDEELWEE
                     DPREELVTQLLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKK
                     IDLIRAMQKILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESP
                     SKSEVVTTFMALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(21597..21968)
                     /locus_tag="ONT85_001815"
     CDS             complement(21597..21968)
                     /locus_tag="ONT85_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCR2378784.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(21987..22874)
                     /gene="xerD"
                     /locus_tag="ONT85_001816"
     CDS             complement(21987..22874)
                     /gene="xerD"
                     /locus_tag="ONT85_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HCR2378785.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
BASE COUNT         6467 a         4926 c         3911 g         7761 t
ORIGIN      
        1 aaatcttgtc acagtccctt attctgaata aacggtgtgg aagagacagc ctatcagtag
       61 taataagaca catctctttc acagcctcat ttttgcgtaa aaatcgattt attcgaaaaa
      121 ttccccgtat acatcttcta cacgttgatc tggattgatc acgatataca agttacctgt
      181 tttttcgctg atttttgttg attgatattg attgtctaat ccttctgttc ctttatctag
      241 atatgggaag tacacaagat caggtgtgcc atttcggtaa aggatttcca ttcgctcgat
      301 atcttcgtat ttcaatgcgc gattaaacat gcccatttcc agtccgccta ggttgatatc
      361 atgtgtcgca actgcgtccc cttcatggaa gatttcgatt ttaaatccag cacacggatg
      421 gatttcaaca aattcactgc cattgattcg tccaaaactt gttgtgattt gtttgatcca
      481 aagatcgccg atatgacggc ggttgatcgt ccaggtttct ccatttctaa aataaaattt
      541 tagcgctgtc atttctttac tcattaaaaa gactccttta tgatgtaatt tattcttgta
      601 ctacctcagt ttagcacaaa tttatgtaag tggtcacatc ttatgttttt tcttctaatg
      661 acgtatctgc ccactttaag tagccaacaa agctgagcag atacagtcta gtgtttttta
      721 aatcaatttt tctttcgata aaaaatccaa aacctgtttt ttatcatttt ttattctttg
      781 ttctacgacg gctttttcag ctaattgcaa agcttcacta acccacttac caggtttttt
      841 tccagtttct tctagcaaga ttttaccatc tactgctaat tcgcttaatg acttgatagg
      901 taaacgatca taggattctc ctacggcatc catctgaggt gtttcgtcaa aatagatcat
      961 caattcttct acttgcagtg catgtttttt gcctaagaag tacaattcga atggttccaa
     1021 agctccagtt tctcgttgtc gtagtccttg gacaagtgct tgaacgtctt taatcatttg
     1081 gttagaacac ttccacgatt tcaaaaagga acgaatctca ttttctttta gatccagttg
     1141 gctgatcaat aatgcccatg cttggctctc ttctttcaat gggacggatg gtaattcagc
     1201 aaaacgtaaa agtgcttcgc cttcttcttt caaaccggga cagcaaatgt agcactctgt
     1261 ttcaataaat gcatggattc ccgcggcccc ttcatttccc aagagcatct tgatgaattc
     1321 cacatttata cgttctacag atattttacc taatagctcg tggttttctt tgatggcgta
     1381 gaaggtttct tcctctaatt tgaaccctag ttgactgaca aaacgcaacc cgcgcatcat
     1441 tcgtaaggca tcttcatgga accgctcatg aggattgcct acagctcgca agatctgttc
     1501 ttctagatct tgcagaccat cgaacaaatc gatgatttct ccatcttctt tcaaagcaaa
     1561 cgcattgatc gtaaaatctc ttcttttcaa atcttctgcc aacgaacgaa cgaattcgac
     1621 atgatctggt ctacggtagt cctgataagt tgattccgtc cgaaatgtgg tgatctcata
     1681 ttgttcatca ttttctaata ccaacacagt tccatgttcg atcccaatat ctatcgtttt
     1741 tggaaaaagc tgtttgattt cttctggaaa tgcgcttgtt gcgatatcca catcatggat
     1801 tggctggttc agcaagatat cacgaacact tccacctaca aaataagctt catatcctgc
     1861 attctctaat tttttcagaa ctggaattgc cttttggtat tccagaggta attgttttaa
     1921 tttcattttt ccaacctttc ctgcgtgttt cttgtcctga gataaagact cagttgcatg
     1981 gtgactgttt gacaagtccg tttcagagtc ttcaaggcca ggaccacttc atcttgttta
     2041 ctcgtcctta cgctcaaagc gattatggtc tctttgattg aatttttcca tgtttttttc
     2101 aaaaacttca gttaagtcga tattcaatga attagccatg atcatcgtaa cgaataaaac
     2161 atcaccaagt tcttctgcta ccgagttaac tggttcagaa gcctttttgc ttttttctcc
     2221 ataagtgtgg ttgacttctc ttgccaattc acctacttct tcagttaatc gagccatctg
     2281 agctaatgga gagaaatacc ccaccttgaa ttgctggata tattcatcca cttcctgctg
     2341 catactgtat aaagaacgct ttttatccat ctttttccct ccaaaactat cttaccaaaa
     2401 ctttaacgca aatcctagat aagagcaatt gagaaaatcg aatttccatg ctacatttag
     2461 gaaggaattt ctaaatggaa attctgtgaa aatacataat tgaaagctgg aattcatatg
     2521 gtcaaacgaa aaacagtaaa agaagttctt tttatcatta taggaacaag tatttatgca
     2581 tttggtcttg tttacttgaa catcgctaat catttagcag aagggggagt ctcagggatc
     2641 accttgatct taagagcttt atttggtatt gatccagctt atacgacttt attaatcaac
     2701 atcccattga ttttactagg aggaaagatc ctcggtaaac gttccttggc ctatactgtt
     2761 ctaggtacac tttctttgtc attcttttta tggatctggc aaagggttcc actagaaatc
     2821 aatttacagc atgatctact gatcgtttct ctacttgcgg gtttagttgc aggtgtagga
     2881 agcgggatcg tctaccgcat gggtggaaca acaggcggca gtgatattat cgctcggatt
     2941 ttagaacaaa attacggcat cactatgggg cgttccctat tggcatttga tatcatcgtc
     3001 ttacttgctt cattaaccta tatcgacttg aaacgtatga tgtatacgtt gattgtttcg
     3061 tatgttttca gtcgtgtgat tgattcgatc ttagatggcg gatattctgc aaaaggtcta
     3121 cttgtcgtct ccaacaaaag cgaagagatt gcaccactac tgatgaccgg tctgcaaaga
     3181 ggtgtgactt tcctttctgg agaaggcgca ttctctggta attcaaaaaa aatcatctat
     3241 atggtcgtta gctctggtga attaactgaa gctaagagga tcgtccacga gattgacgaa
     3301 aaagctttcc tttcaatcat caatgtgcat gaagtggaag ggcaaggctt tacttatttg
     3361 aagccacaga caagattact gaaaaaacta taagaaaaaa gacaaagata ggctatgagg
     3421 aaagattcca catccagctg acctttgtct tttttagtac tatactattt ttaactaatt
     3481 gttatgttca cttacccggt tacttttctt ttgtgtctgc aaaaggatct ttcggttaac
     3541 ttcatcagat aatttcaaaa aagcatccct atctccatct tctagtgctt gatcgatttg
     3601 agcataaagc atttttagtt ttgcctcttc ttctttttct tgaaaatatc gttcaattaa
     3661 ttcagtatct tcttcactga ctgaagaatc ccaagaagaa aatggattgt cttctaagat
     3721 agatagatat tctcttgtct gccaagcatt ttcgaatata cattccacat ataaaggctt
     3781 tttccaattc aaacggattt catgaaaaat ctggtctgtg tccgtaaatt cttttccctg
     3841 caaaaacaat ttcatgggtt ccccttcaac agatagatca ctgatctgca gtcctcgatc
     3901 cgcaaaattc gccccttcaa caaagtggac attgcttaaa atcgcttcat gattggcaag
     3961 ataattcaag atccaaaaaa cttctcttcg gctaaaagat acatgattga ccatccacgt
     4021 taaaaaattc ttcttttccc gtacatcaat tagcattttt ctcacctctc tgttaggtca
     4081 tctaataatg acagtgcctc catatctcct ggttcaagtg ttaaataata agtcaataat
     4141 tcttgcgctc ttttcagctg cccttcttct cttaagaaaa aggcatactc tttcaagaaa
     4201 tctggttcat gtgatagttc atgataagcc tgttcatagt gtacagctgc aacggtaaag
     4261 tcttctaatt cattataagc atgggctagg ttccattctg cataaggatt agcagtttct
     4321 tccatctgat tgatcacttg tatcacatct tcatatcttt catcatccaa gtaaagatta
     4381 cttaaagtaa gtaatgtttc atcttgtttg tctcctactt ctaaagcttt cagtaaaagt
     4441 tcttctgctc gctgtctatc atgcagacgg aaagcatttt ccgaggccag gtgataaagt
     4501 tcgacttggt aaggattttc tttgattcct tcttctaaca ctgtttgtgc ttcttcaatc
     4561 atttcttctt cttgaagagc ttgtcctaga tatagatata acgattgata atgcggatta
     4621 acttctcgaa gttcttccag atagttgatt gcctgctgat tttctttcaa ttgaagattc
     4681 gtaaaagcta gctggaacag tcgatcatct gtcctttctt ctttcagtgc ttcttctaaa
     4741 ggtacgatag ctgcttcgaa ttctccttgc atacttaagg attgtcctaa acgttcctgc
     4801 atcgagattc ctgaaatttc atttatcccg cttgccaaca atgtttcata aaccactgct
     4861 gcctctttga aacgatcaac tgaaaaatac aattcagcta gcgcaaactg gatcaatggt
     4921 tcatcaggaa gcaacccagc tgcttctttc aattttgctt cactcacttc agggattccg
     4981 ataacttggt acaaatcagc tatcgccaat aagctttgcg gatagtaatc actttgctta
     5041 tccacttttt ccaggtattc aaaagctaag tcgatctcat tatcttcgat tgcaatttct
     5101 gctaacggga tgttcagtcc gtcattttct gggtattggt tgatcagttg ttcaaaaatc
     5161 tgttttgctt cgatcaaaaa gccaatagcc agcaattctt cgcctagttc agctaatata
     5221 tctgctgggt cttcttttaa tgctttttct aaaaagattt gtgcttgtga caagtcttca
     5281 tctgataaag cttgcaacat tttttcgcta tttgtattca tttcaattcc ctcttatctc
     5341 actgacgatt tgttgaaata ccgtcattgt tctcaataat tctttttcgt ttggatgttg
     5401 gtatccgtct attataccta ttttttctaa aacaagtatt tttttgcttt tttctgccaa
     5461 aaaaagaact ttttccaaat actccatctt tgaaatctgt tccagccagt ggattggata
     5521 gccaagcaag tccagccaca caaaaaagtt ttttagttgg aaatcaaaag gatatgcttg
     5581 gagattccac acgaaatgaa acatcattcc taaaaatttt ttcatactag cagaaagtag
     5641 atggccattt tcggtcagat aaaaagcttg ttcaaacagt ttaccataat ttgacaggtt
     5701 ttctgcctct aattgataaa aatagatcat ttcttcgatc atcccggcaa acggccgagt
     5761 cgttaattgc tttttatttg gatagttttt atacagattt tgcaaaaaac gatgatcaca
     5821 aatcattccg caacagataa aagtcaaaaa gtcaatctgc tttccgtcca cctgccgctc
     5881 gctgattgtt tgatcataaa cgatgcgttc tggcagattg atggtttgca gcactacacg
     5941 atttggtagc tgcaaaatcg tccgctgcgg tgttaatgct cggcttagag aacggatcga
     6001 tacagggatc atccatagtg atgaatcaag ggttgtatgt ttttgaaaaa aacctgccag
     6061 atccatgatc ccttcatttc caaatccgat gataaacatg gattgatttt cgggatagcg
     6121 tttgacgaaa tcaagcaggt tctttaattc tgttaaatga ttgcattgag tgttggcaca
     6181 aatataccag tcattgtttg cttgatttgc aaataacggg ttgatcttat ctgcaaacaa
     6241 atcataatag cgctgattcg ttaaaaataa gattggctga tcaatggaaa gttctcttag
     6301 ctgactgact agcgtttctc cgtaaaacac tttcgtcaac gcatcttcta cttgataatc
     6361 aagttccata cgcatccctc ctcttcttat ttttatttta ccattagtaa gcaaattcta
     6421 gaaaagataa gagcaaagct cacctcataa cttggaaata aataaaaatt aaataaaata
     6481 catgtgtttt ctatgtatga catacggatt cggaatctct gtctcttatt tattcctttt
     6541 ctgctaggaa ataaaaagaa aagcacttgt agccctagag ccacaagcgc tttttcaact
     6601 gtcttattta acagcgtctt ttaatgcttt acctggtttg aatgcaggta ctttgctagc
     6661 tggaatttcg atttcttcac cagtttgtgg gttacgacct ttgcgagctg cgcgttcgcg
     6721 aacttcaaag ttaccaaaac cgattaattg aactttttcg ccgttagcta aagcatcttg
     6781 aatagttgaa aatacagcat ctactgctgc agttgcatct tttttagtta aatcagtagc
     6841 tgaagcaact ttttcgatta attctgcttt gtttgccatg gatagattca cctcctccac
     6901 aagagatatg atagaaatat catttagtta aaactttctg agtggtccag aagtttaaaa
     6961 agaaatattg attaacttca atattctgtt atcacgatat catatgaacc gcttaatagc
     7021 aaggttttta ccaattttca catgcttttt ttagtcatag aaacgtaaca aacgaaactg
     7081 tctttttctt ttgaaaaaaa aacgtcgata tggtaaaatg cctattttct cctacgaggg
     7141 ataattcgga tggacgtccc ttcaaaagta aatgcttttc tgatttgatt ttctaagaaa
     7201 cgttcataag aaaaatgcat caattcctct tcattgacaa aaacgacaaa tgtaggtggt
     7261 ttgacagcga cttgtgtagc ataaaaaatc ttcaggcgtt ttcctttatc tgttggtgtt
     7321 ggattgattg ctacagcatc cataatgata tcattcaaca cagccgaaga gatacgaaga
     7381 ttctggttca tacttacttg ttcgatcatt tcaggcagac gttccaaccg ttgtttagtc
     7441 actgcagaaa caaaaacgat tggagcgtaa tcaagatacc ggaattcatc acgaatctct
     7501 tgttcgaaat cacgcattgt attggtttct ttctttacaa gatcccattt attcacgacg
     7561 ataataattc cacgaccagc ttcatgggcg taacccgcta ctcgcttgtc ttgttcccga
     7621 atcccttctt cagcattcag taccatcaaa acgatatcag aacggtcaat ggcacgcatt
     7681 gcacgcatca cgctgtattt ttcagtattt tcgtatactt ttccgcgttt acgcatacca
     7741 gctgtatcta tcattaagaa tttctgacca ctttcagaaa cgaagtgggt gtcgattgcg
     7801 tctcgggtcg ttccttcaat atccgaaaca attacacgtt cttctcctaa aatcgcattg
     7861 atcaaagagg atttaccaac atttgggcga ccaatcaaac taaacttaat cgtgtcgtcg
     7921 tcctcttctt cagtctcttc agagaaatac ttcactgctt catctaaaat atcaccaatc
     7981 cctagtccat ggcttcctga aacaggataa ggatcgccta gccctaagga ataaaattca
     8041 tagatatcgt ttctcatttc tggattatct actttattga ctgctaaaat cactggttta
     8101 ttacttctgt ataaaatttt cgcaaccatc tcatctgcgt ctgtcacgcc ttcgcgtccg
     8161 ctgactacac aaatgattac atctgcttct tcaatcgcga tttctgcttg atgcttgatc
     8221 tgatccataa aaggttcatc gccaagatca attccgcctg tgtcgatcac gctgaattct
     8281 cgacctaacc attcaccttt tgcataaata cgatcacgag ttactccagg agtatcttcg
     8341 acaatcgaga tacgctcacc agcgatgcgg ttaaaaatcg tagacttacc gacgttcggg
     8401 cggccaacga ttgcaattgt tggatttgcc atattatttc ctcctcacaa gtttccaatc
     8461 tcacttagtt taacgaatgc attgccaact ttcaagcata gattgtcaat taaacgaagt
     8521 atttttattc cttattgaaa tactaatcgc ttttcaaaag aaaaactgag acaaaagcag
     8581 ctgctttcat ctcagtcaca gatacaaaat caattatttt tcagaatcat cggttgcttt
     8641 ttcttctact tcttctttca acgcatcacc taaaatgtct cccatagtaa atccagtatt
     8701 ttcttctggt aattcataag actcaacatt ttttggttct tcttctgaag ctggtttttc
     8761 ttctaaagct ttgatactca atgcaatacg atgttcttct ggatggatct ctaatacttt
     8821 cactttcact tcgtcacctt catgaagcac ttcatgtggt gtcgcaatat gtttatgtga
     8881 aatttgagag atatggacta gcccttctac tccagggaac acttcaacaa aggcaccgaa
     8941 gcttgtcaaa cgtttaactg ttccgtctaa tactgtgcca actggagctt ttgtatcgat
     9001 ttctgaccaa ggtccaggct gtgtgtcttt gattgacaag gaaacacgtt cttgttcagg
     9061 attgatagac aacactttga catttacatc atcattgaca gccaatacat cacttggttt
     9121 tgctacatga ctatgtgaaa tctctgagac atggactaga ccgtcaattc cgcctaggtc
     9181 gacaaatgcg ccaaaatcag taagacgtgc aacacgtcca ctgagaacat ctccctcatg
     9241 gatagttgaa agaatttctt ttttcttttc ttctctttcg ttttccacta ccgctttatg
     9301 cgataagatc aaacgatttt ctgatggttc gatttcgatg attttaaagc tcaatgtttt
     9361 gcctttatag tctgaaaagt ctgcaacaaa atgatcttcc accattgatg cagggacaaa
     9421 accacgtaca ccgacgtcaa cgactaaacc gcctttgaca acatttgtaa caggtgcttc
     9481 gatgaccttg ccctcttgga aatctctttc gatatcttcc caaacttttt tagcatctaa
     9541 acggcgtttt gaaagtaggt agctgccgtt ttctttatct ttgccgatcg tactgataac
     9601 gactaaatct aatacatcac cgacttttac tacttcgctg atatcttcta ccggcaaagt
     9661 tgataattct tttgccggaa cgactccttc aactccagcc ccttcgattc cgacaatcac
     9721 ttgtttgtct tcaatgacta aaacttcgcc cttaacaaca tcgccaactt ttacttcttg
     9781 tacgcttttc attgcgtctt ccattgactg gttgttctct tgattttgat caaattctgt
     9841 cataacactt gtcctcctaa ccaacattct gccgtaagta cggtactgtt tttagtttaa
     9901 ttgattattc aaaaaaaata aagcgatttc gttcgttttc cttgaaaaaa caattgtttt
     9961 tcattttttt tcttaattta aaaaagaaga taaccttttt tcaaaacaac agcttttatt
    10021 gctgcaacga cttctggaat cgatttgcca gtcgtatcga tacgaacagc atcctctgct
    10081 tgcttcagcg gagagacgtc ccttgttgaa tcataatggt ctctttcctg tatctctttc
    10141 gtcagtgttt caagatcagt cggaattcct ttttcttggt tctctttgaa acgtctttct
    10201 gcacgctcac ttacgcttgc tactaaaaag attttcactt ctgcattcgg taatacagct
    10261 gtcccgatgt ctcttccgtc catgacaacg ccaccgcgtt cccctatttt tcgttggaca
    10321 gcaactaatt cttctctgac ttttccatgt gcagagactt cagaaacagc ttttgtgaca
    10381 tctggttgac ggatttctaa agtcacatct tttgatgcta tgaatacatg ctgtccatct
    10441 tcttgttgcg aaaaagagat aggatacttt ttgatcagcg aaactagccc ttcttcatct
    10501 gaaaaagata cattgttcgt aatagctaaa taagttactg cgcgatacat ggcaccggta
    10561 tctgtataaa tataattcaa ttcttttgct aatatcttgg caacggtact tttcccagat
    10621 gaagcaggcc cgtcgattgc aatactgatt tgtgtcatga tataactcct ttttctcaga
    10681 aaaaaagccg caaagcgcgg ctattattat ttaattctta attcttgacc agggtaaagc
    10741 ataaagttgt ttggatctaa gccattcaat tcaaacaatt gatcagtggt gatccctgca
    10801 cgctcagcca cttgttttgg accttcgcct gcttgaacag tcgttgttga accggctgca
    10861 gctgatgaag aaggtgttgt ttctgctgca ttgctgcttg aatcccctgc ttgataagtt
    10921 gcctcacttg aggaagtcgg tgttgtctct gctgcaatac tgctttctga cgttacacct
    10981 gtatcttgag acacagtaga ttcttccgcg attgtactag attctactgt tgaagtctca
    11041 gttgactcct cttttgttgt acttgtagaa ctttctacta ctgatgtcga agtttcagaa
    11101 gaagttgctg ctgttgcatt gttgcttgat ccatttctga tccagaaata agcacctgct
    11161 ggaatagcga tacataagac cagtaaaatc aaaaatagcg aaagaaataa tgtatttcct
    11221 ttcttttgtt gtctctgaga acttcttgat gaaacttcat catctgtatc gtagattggt
    11281 tgttcccatg gttcttgtgt ttcgttattt tctgtagatt gatgtctatc ttttctgctc
    11341 actgttttac ctcctaaact ctatcgtgtg tattatacca taggaaaaag tgtcttgtcc
    11401 taaaaaatca atgttccatg ttttttttaa ataaatttag taaaatcgtt tcccaatgaa
    11461 gttgttcagt cttagaaaaa agcttcctct ttggcagaag ttctgctttt gctccatcaa
    11521 tatcacaaca attctgcggc ttttcggaga gtttttcgcc aaaataagct aagatgaatt
    11581 ttctgcggca gttttcttca ttaatatagc gaagcatctg ctgcaggcgg aattcttttt
    11641 ctttttcctg acgtttgagc cgttccaccc aattttctgg tttttcagac tgctggatca
    11701 gctccatcca ttttttttgc agctcatcaa agggagcttg ctttgtggca tattccaaat
    11761 aaagctcaaa actttgtcgc tgttctctag aaagttgatt aaagaaataa tggatacgtt
    11821 catccccact ttgataaaaa agaattgctg cactttcttc ctgatctctg cctgctcgac
    11881 cgatttcttg tacatagttt tctagactgt ctggcaaatc ataatggacc acataacgga
    11941 tatcagattt atcgatcccc ataccaaatg cattcgtagc aatgagaaac tgcagctgat
    12001 tttttacaaa ctgctgttga agttgtcttc tttgagctgc atcaagacca ccgtgatagt
    12061 atccaactgt aaaacgttcc ctgaataaat gatacaatcg ttcgacttct ttttttgttg
    12121 cacaataaat gattgccgct ccttgtgctc tttccatgaa gtgttcaagt tcttgttctt
    12181 tttgctgcgt tttccgaaca aaaagcgaga tattttgccg attgactgat tgtctgatct
    12241 cttttggtgg tcgatcaaat agtacatgac gtatatctct tgctactaaa tctgttgctg
    12301 tcgccgttag tgccagtgtg accgggtttc ctaaaagctt tcggattttt cctaactgtt
    12361 gatattctgg acggaaatca actccccatt gagacacgca atgagcctca tctactacat
    12421 ataacgcaat attttgtttt ttcagatgct ctaaaagaga aggattagtc aacatttcgg
    12481 gactcaaaaa caaaaattta tattgcgata gatgacggag gacgtatctt ctttcagatt
    12541 gcgaaagtaa actattgaaa gcagctactc gtttctcccc tcgtttttgt aattgcgtca
    12601 cttgatcttc cattagagaa agcagcggtg acacgatgat gaccagacct tccattaaat
    12661 accccgtcaa ttgatagcaa aggctttttc ccgttcctgt aggcaaaata gcgagagtat
    12721 cttctccatc aagcaacgcc tgaataattt cttcttggcc gggacgaaat gaatcaaatc
    12781 caaaatattt ttttaattca tttattaaca ttctgtatcg cttcctttct caatagatag
    12841 atttgataca aacgaaattc tccataatct aattgtccat ctacattcca ttcgctaaag
    12901 cgccactctc gcacggaatc cttatttggt tccaaacgtt cgattgtttc tgcagagaga
    12961 atccactcgt atggaaaatc atcaaaaaac aaggcccact caataagatg atctgttact
    13021 gttcctcttt ttaaatgacg gattgccatg atctggtcaa ttgtctgacc attcataaac
    13081 atctgcttgg tgatcatcat gctttgattg aaattttgct gtaataatga atcgatcaaa
    13141 gcgtgccaaa gcgaatctgg ccgtttttcg atttgtgcta acaaaaggtg gatacatctg
    13201 ctttgttgca gataaacctc tgactcatca ttgttttttg ccagctgata aggaagcagc
    13261 cctgttcgct catttccaga taattggtta gctaaaaaat ctgctgcttc ttctggtatc
    13321 tccgaaaaca gctgcaccaa actctcatac gcagtatcaa tcaaaatttc acgtggcaat
    13381 tggctttcac taagccattt ttttagttgg aaagtataaa aaggacttgt ctctgctgga
    13441 agatagtctt gaatacccgt tgccagatta gaaattactt gaacagcaaa tttaatgagc
    13501 cgccaactag tttcccctgt tctgccatag cggtcaaaac gcaaaccagt atgttctatt
    13561 tgatgttcgg acaacatatc agcccctgca ggcgttagct ttgcttcatt ttcatttatt
    13621 tcgatccaac cttgctgcgc aattttttgc aaaatttgat aaaacttatc ctgcttcaat
    13681 tctggaaaac tgttatggat aaacaaaagt tcgtgggtaa atccataaat aagtactgaa
    13741 gtcgttcgtt ttcccacaag gagttggtaa agagaactaa tccgcagctt gtgtcctgat
    13801 gaaaatagcg ataaaataaa aacaatcata ttattctcct taagttagga tggttagaaa
    13861 tgtcactact atgtaaactt gtacctgaac gctgtattgc atgcggtctt tgtcaaatcg
    13921 aagcgccaga tattttcgat tatgatgaag aaggaatcgt tttatttgcc caagaaccag
    13981 aggctcatca acagttcgtg atgccggaag aagaaacata tgtaaaaaaa gcgtatcaaa
    14041 aatgtccagt tcgagcgatt ttattagaaa ataaaatata aatataaacg aaactttaaa
    14101 aacgtacact aagttctcta aacttgaaaa taagccggga tctgtttctc ttgttccgtg
    14161 aaatcccgac ttattttgaa agattcattt gctatctata gttaattgac tagttttttc
    14221 acggacccta taggacccat ttatattatt tcaacttttt cagccagttt tccaatgtat
    14281 tgacgatcac agtttgctgg tctgcgttag aaatttctgc ctctccatct cctttttgct
    14341 caccatagct gccaaaacct ccatgatttc cgccattgat tgatacatac tctgttcttt
    14401 ccggtaaata ctgcttagct tcttgataag cttcctgatt caatacttta tcttttgtcg
    14461 ccgtgatcga taaaacagga acagctgatt ctgtcaatgt tccttttttg tcgggataac
    14521 ttgccatgaa gaatacccct tttaaatgtt tgtctgaatg attcttattg acataacgac
    14581 ttgccattac gccacctaat gaatggccac cgatcacata atcggaattt ggaaaatctt
    14641 ctttgacctt atccgctttg tttccgccca tcactgctag atcaagtggc attttgacaa
    14701 tagcgactga aaagccgttt tttgctaaat cttgcgccca aatactataa ctttctggct
    14761 ctacgagagc accaggataa aaaataagta atggagactt tccatgattc tttttctctc
    14821 cttcaaaaaa taagtaatct tctttgtttt ttccttcttt taccaacact tgcgcttctg
    14881 acgatggttc gtacgtattt tgctgtatat agtaataacc ggctccaccg agtaatccta
    14941 aaatgatgac taagccaatt agtattcgtc taaatatttt cactttctcc caccttttct
    15001 atctgagtga cccctttttc ttaatcaagt atgattatac cttaaaaaaa taagagagaa
    15061 atgcaaactt tccagcatca aataaaaaaa gacggaatat ccatttgaat atccgccctt
    15121 tttgtatgtt tatttttatt tagcacataa acgatacaca gcttctgcat aaatttccat
    15181 tgctttatac atactactta attcccatcg ttcattcgcc tggtgcataa agtcaggagt
    15241 atcagggaac atcgcaccga atgccacaca ctggttcatc gtacgagcaa aagtagcgcc
    15301 gcctgataca aacggttcag tcatgtctcc agtaatatcg cgatatgtcc ccaatagatt
    15361 tttcactaac tcactgtcta gtggcacgta taatgaatca aggaagtcaa attcttcata
    15421 agtcaaccca tatttttctg ctgttttagt taatttagct actaaatcat ctttcttgaa
    15481 tgtaacaggg attctcatat ctacaccgat ttttgtttgt tctggtgtga tttccaaact
    15541 tgcaaagttc atcgttagct cgccggattg ttcatcttct gttttgccca cgacagcttc
    15601 accagttgca ttttcttgaa ctaattttcc taagaaattg attgggccaa aatcaaatac
    15661 ttctgaaaga gcaattgcca gacgagaaat agcattcact ccttgtgctg catctttcgc
    15721 gtggatactt ttaccaagaa cgacgatgcc ttcaccttgt tcttcgaaat cgaattcgtg
    15781 ttttttcaat gcttctttta cttcagaaag cttttctcca gagtaaggag ctgcatcagg
    15841 aacaacattc aacgctcctc cagcttttac agaaaactcg ttcgttcctg gtcctgtcaa
    15901 gtaagcttgc aataagcctt tttctgcata aattaaagga aattcagcat ctggcgcaaa
    15961 tccttgcgta attccttctt ctttttcgtt atatttttct aaacaacgcc ataaattttc
    16021 ttcatctgta ccaaagataa aacgaatacg tgtcttgaat tccacgccag catcaatcaa
    16081 agctttgacg gcatacattg ccgcaataga aggacctttg tcatcttggg atccgcgacc
    16141 aactaaccag ccgtctttta tagttggatc gaatggtttt gtttcccaat tattttcgtc
    16201 ccctgctgga acaacgtcca tatgacacaa gataccgaat aattcatctc cagagccaat
    16261 ctctgaataa ccgtaatagc cttctggatc tttaaatgtt ctaaatccta gcttttcact
    16321 gatttccaat actttatcca aagcttcaat cactttttta ccgaagggat gtccttgtcc
    16381 tgaatctgct tcatctagta cggaaggaat tcggatcaat tcgctcaatg attctaatgc
    16441 ttgttcatga tgttgttctg tcacaaattt tttcatgata ttatcctcct aatatgttaa
    16501 aaagggtcat gtcaaattcc tccgacacaa cctcttcttc tcaaataatg acgttttaag
    16561 cctgctccat cagcaagcag aatgttctta acaaatgatg aaagaaaaaa cactcactga
    16621 atggagctat tgacttgtcc acgtgcttat cgatagccgt ttatcggttt cttcattctt
    16681 atgatagacc ttgtacctgt atttgtctag gtttagaaca ttgctgctac acctaggatc
    16741 acacagatgg ctacaaataa tatcgcaatt aatttaccgg taaatttcca ccacactgaa
    16801 acatcgaaac gagcaattgc tacagcaccc attacgatag cagatgttgg tgtaattaag
    16861 tttactaaac cagatgcaga ttgataagca gtgattacta aatccttacc gacgccggca
    16921 aattcaccca taggtcccat gattcccatt gttgcagcag ctaagccgga agttgaagga
    16981 atcaagaatg acatagggat atagaaaata aatgtaataa tgatgaatac acttgaagat
    17041 aatgaactta atccttgttc gccccaatgt aagatggtgt ctgtgattaa tccattgttc
    17101 ataaccactt ggataccacg agcaacagct actacgattg ctacaccgat caaatcagaa
    17161 gcaccagcta aaaattcaga gacaaattca gactctttca tgccataagt gattgctact
    17221 acgatggaca ttactaagaa taacatagta atttctggga agtaccagtc acctaatgga
    17281 agcatgcttt tacctagaac cgtacctagt accggaaggt ttgttaacca gtttgtcaat
    17341 gattcaaaaa tcgtgaaatt cgcattcaag gttgtccaag gaatcaaact gatgatcatg
    17401 ataccaaaag taataaagaa caatacatta acatgttttt gttttttcgt gattacttct
    17461 tgacgaccga ttgcttcaat atcgaaatgc ttcatatctt ctttacgttg gttgtatact
    17521 aatgattttg ttggatcttt ttcgatcttt gatgcataac gatacacata tacaatagaa
    17581 atagctaaca aaataacgaa catcaagaat cgaagaccaa taccttcacc catcgaaatc
    17641 ccaacagctt gtgaagctac gcctgttgca aaaggattta ccgttgaagc tagacagcct
    17701 acttgagacc ctactaatac gattgctaca gctactactg tatcaaagcc tacagccatc
    17761 atgactggga tcagtaaagc atagaatgga atcgtttctt ctgccattcc gtaagtagag
    17821 ccgcctaatg caaataacag catcaatact ggaataagca ttttttcttt tcctttgaat
    17881 ttcttcacaa ctgaagcaat cccggcatct agtgcccctg ttttgttgat aatacctaaa
    17941 aatccaccaa caaccaaaat gaagaaagag attgggattg cagcaggtgt ttctttagtt
    18001 agctcccccg ctggtacacc tagcatccca ttgattggcg ccatgaaaat atcccacagc
    18061 ccttgaggat tttgttgtac tcgttcatat gatccagcga cgatgttgcc tgcatcgtct
    18121 acactatagt gcccagctgg tataaaccaa gtaagtacag cgattactgc aataataata
    18181 aataagaccg tatacgctga aggcatttga aattttttct tttttgtttg ttccatcttc
    18241 ttcttccttt cttttccgtt agagacaaca attttggctc taatgttatt ttatcaaaaa
    18301 gagtatcaga acaaaaataa taacacgcat tttgtaaaac ttttgttcaa aaagttcgtt
    18361 tttccctatt ccctcctctt ttttcagttt tttaaagctt tcttcttttt cattgtagcg
    18421 tttccttttt tttataaaca aacattttcg tcttttaaaa ggagaagaat ttcacatttg
    18481 caaagaattg attgattttt atgcataaat caaaaaaata ctcaaaacaa acaaataaaa
    18541 cgtttacatt attgcatgtc ttgtattttt ttccaaaaaa aaagaccaag aaatttcttg
    18601 atctttttga tatttatttg actgctgtat gacgttgttc gaactgatgt tgttttcgac
    18661 ttaaccatgg aagaagttgc gcatgcagca ctaaaaagac agcactcacg atgaatcctt
    18721 tgatcaagtt gaaaggaacg atcccaatca gtacatagtt tgctaaagac atgcccagca
    18781 tctgtgttgc gttcagccct aaaaacatta agtaaagagg cgtaataacg aaataatttg
    18841 cgatactcat aaaaatagtt agagcgatgg ttcctgtaat gacacctaaa aatttgtttc
    18901 ttcctttatg ctgtttcttt acactaaaga aataataaat cgggaatgta aagatcgttg
    18961 ttgataagaa actagcagtg tctcctacca aattatccgg agaaaaacct gtagtcagca
    19021 gatgtaatac tgaacgcaag aacgctgttg caactccggc cgctggaccg aataagaaca
    19081 tgctgatcaa taccgagata tcactaaaat cgatcttcaa aaaactaaaa gccggcatga
    19141 tcggaaaagc aaaatactgt aaaactaatg cgatcgcagc aaacatcgct actgcaacca
    19201 ttttctgtac acgggtgttc ttcatttgaa atcctcctgt taggactcct cttcagcgaa
    19261 ctttttgacg tttctctcca aatgaaaaaa ctctattttc cagggaaaat agagttagag
    19321 ttatttgatc aagcgtcgtc aaataagctc tatcttcttt atccagacta tactgtcggt
    19381 atcggaattt caccgattca gctatttttt tgcaaaatag gtcgtggact ataaccaccg
    19441 gtcgggaatt tcaccctgcc cctgaagacg aaccttatat aatttttgtt tacatattca
    19501 ttatacatat ctattcaaaa aacgcaacat ctaacaatta taaaaagtaa gtgttcattt
    19561 tgagtggttc agcaaactgc tgacttcttt tttgttcagt tcacggtaat ccccaggacg
    19621 taggccttgt aaagtcagat ctccgtattt ttcacgtttt agtttttgca caggaagacc
    19681 aactgcttga agcatatttt tcacttgatg attacgtcct tcgtggatcg tcaattccac
    19741 gatactgtgg tttgtcttag gatccactga taaaatttgg aatttagctg gagatgtttt
    19801 tcgtccttcg atctttattc cttttgtcaa aggtgcaagc atcgattttg tagcgatccc
    19861 ttttaccttt gcaacgtaaa ctttatccac ttcatgtttt ggatgtgtca ggcgctgagc
    19921 aaaatctcca tcatttgtca gtagcaagat ccccgaagta tcgtaatcca gtcgacctac
    19981 aggatagata cgttctttta ccatcggcaa aagatccgta acaacttttc ttcctttgtc
    20041 atctgcaaca gcagaaataa ctcctttagg tttgtacaat aaatagtaac cgtattcttc
    20101 ttgataaatt gggacttcat ccacttccac agtgtcatgt ttcccaactt ttatccctaa
    20161 ttcccttaca acttgtccgt ttacttttac acgtcctgca aggatgaatt cttctgcttt
    20221 tcgacgagaa gtgatgccag cgtgggcaat tactttctgc aatctctcca ttacaattat
    20281 tctcctttat tcattccatc aaagaataga tctggtgaca cgtcttcttc aagtgaaatt
    20341 tccatttctt gtgtatcagg cagttcttct aatgatttca aaccaaaata atctaagaag
    20401 tagccagttg ttccatataa tatcggccga cctggtcctt ctactctacc tttttcttca
    20461 atcagtttat gggcagctag acgctgtacc gctcctgaaa cctgaactcc tcgaatctgc
    20521 tcgatttcag ctcttgtgat tggctgttta taagcgatga ttgctaaggt ttcgacggcg
    20581 gcttgagata atgtgtttga tgtcccttgc gcatatcgct taagtaaggg agccagctct
    20641 tttttagtcg ttaatacata atggctttcc gtttcaaaaa tatgcaaagc tgagtgcgag
    20701 ttttgagtgt aagattcttt taattctttc actaaacttg tgacttctgc ttcgctgaga
    20761 tttaacagtc cgcttaattc ttcattactg atcccttcct cgccaacaat aaaaagaagg
    20821 gcttcaagtt cacttaattt cgtcatgttc ggtatctcct actgctggat aaagcataat
    20881 tgattcatag ttttcttttt gttgcgcaat gatctgccct tttttcatca attctagcaa
    20941 agccataaaa gtagtgacaa cttcactttt agaaggtgac tcaaaaaaag aatcgaaagt
    21001 acagccatgc gcatttcttt ttatacgctc ttcgataaat accattcgtt cttcgatcgt
    21061 tgtgtcatcg ggcgtgatcg ttttttccac tttttgagaa aatttcttac gttctaagat
    21121 tttctgcatg gcacggatca aatcgatttt tttgacttgt cctcgcttca acggcttttc
    21181 agcatccgct aacccatcga tgttcatagg ttcttttgtg taataaagac ttcgttcctc
    21241 tgctttttct gtcagtcttt cagctgcata tttatattta cggtattcta aaagctgagt
    21301 gaccaattct tctcgaggat cttcttccca taattcttca tcgtctgtgc cgaattcttg
    21361 gacaggaagc aatgtcttgc ttttgattgc catcaatgta gcagccatca ctaagtattc
    21421 tcctgcaaca gctagctcga gtgttttcat tgtatggata taattcatat attgctccgt
    21481 gatcgtagcg atcggtatat catatatatc gatttccatt tgctgaatca gatgaagcag
    21541 cagatcgaga ggaccctcga atacatctaa tttaatattg atctccgtca actcattcac
    21601 tctttcttct ttttctttgc tagccatttt ccaatgtaag gctctgtgat ttcagttgca
    21661 cggatggaga catccgggta cttttcctgt aattcatcta acatttgaaa gcccatattc
    21721 tcaccgcgat aagacggatt caaagaaatg tcatggatga tcagctgatc gttcgttgcc
    21781 cagtaaatac caataatccc ttgaatgttg gttgaacctt ctggcgtaaa gaaaaaaagt
    21841 tcataatcgt cttcagattc gtacgtatca atctccttca acaaagaagt tttacctgtc
    21901 agtttcttat ggaaactcaa tagtcccata gcaattttac ggttttcttt ccggtaatgt
    21961 gttaacatct tctcaccttc ctatgactaa gcccttggaa aatgctgctt ataaacatcc
    22021 gtcattcttt tcttcgtgat atgtgtataa atctgagtcg tagagatatc cgcgtgacct
    22081 aacaattcct gtactgtccg caaatcagct ccattttcta gcagatgtgt ggcaaagcta
    22141 tgccgcagcg tatgtggtgt tacttctttg taaatcccag cctctcgtac taactgcttc
    22201 agatttttcc agattccttg ccgagaaaga cctttcccat gattattgac aaatacatgc
    22261 atttcagatg cgtcctttac aagtaatgga cgtgcttcgt ctaagtagcg ttccagccat
    22321 tggatcgcat aatcacctaa aggaatgatc cgctctttat ctccttttcc aactgtctgt
    22381 accagtccaa gagaaagatg aagatctccc aatttcaatc caatcaattc actgacgcgc
    22441 atcccagtag catacatgac ttccaagatt gcacgatctc ttatccctaa cgtttttgtc
    22501 gtatcaggtg cttcaatcag cttttctact tctgtcagag tcaatgtact tggtaatttc
    22561 tgtgctttct tcggcgtatc gatatgttgc atcggatcat gatcagtgat acgctcttgc
    22621 cgtaaaaact gatggaatct tctgagactt gagatcatcc gagtaacagt tgctgtcgct
    22681 tttttttctt catgcaactt ctccagaaaa cggatgacta aaaagcgatc gacatcctgc
    22741 catgaagcaa ttttctgctc ctgtagaaaa tgcaagtact gttccaaatc acgttcatag
    22801 ctttgtcgtg tattggcgga caatccccgt tcaatattca agtagtggag ataatcgttg
    22861 atttgctctt tcataataat ccccctcatg cctctctatc atatcagata ccgcttcatt
    22921 ctgatagtct ttttccataa tctgagcaaa gaaaaagccg tctagacact cagacggact
    22981 ggtttctttt ttcggctcta taatttatgg gactgatgaa tcagaattga ttcttcagcc
    23041 ccattttcat tttaggtatt aaaaa
//
LOCUS       DAJSBL010000038        22479 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.038, whole genome shotgun sequence.
ACCESSION   DAJSBL010000038 DAJSBL010000000
VERSION     DAJSBL010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22479
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.038"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..441
                     /locus_tag="ONT85_001817"
     CDS             <1..441
                     /locus_tag="ONT85_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HCR2378786.1"
                     /translation="KSAVITLVERLSKVIITIKPNGRKALDIETALNQWFSRFPKNFF
                     KSITFDCGKEFSNWKAISNQHDIDIYFADPGTPSQRPLNENSNGILRRNGLPKSMDFR
                     KVNQTFISSVSNQRNHIPRKSLNYRTPIEIFLSYVQEAFYSSLI"
     gene            complement(512..1822)
                     /locus_tag="ONT85_001818"
     CDS             complement(512..1822)
                     /locus_tag="ONT85_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HCR2378787.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     INFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1834..3252)
                     /locus_tag="ONT85_001819"
     CDS             complement(1834..3252)
                     /locus_tag="ONT85_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR2378788.1"
                     /translation="MLSQNAVTANTVKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
     gene            complement(3492..5060)
                     /locus_tag="ONT85_001820"
     CDS             complement(3492..5060)
                     /locus_tag="ONT85_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HCR2378789.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(5064..5345)
                     /locus_tag="ONT85_001821"
     CDS             complement(5064..5345)
                     /locus_tag="ONT85_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HCR2378790.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAAGAFSLYRVRMRKGA"
     gene            complement(5342..6265)
                     /locus_tag="ONT85_001822"
     CDS             complement(5342..6265)
                     /locus_tag="ONT85_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectin esterase"
                     /protein_id="HCR2378791.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(6262..7140)
                     /locus_tag="ONT85_001823"
     CDS             complement(6262..7140)
                     /locus_tag="ONT85_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HCR2378792.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPTVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIETLLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(7127..8464)
                     /locus_tag="ONT85_001824"
     CDS             complement(7127..8464)
                     /locus_tag="ONT85_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HCR2378793.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTSAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(8467..9570)
                     /locus_tag="ONT85_001825"
     CDS             complement(8467..9570)
                     /locus_tag="ONT85_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HCR2378794.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(9644..11152)
                     /locus_tag="ONT85_001826"
     CDS             complement(9644..11152)
                     /locus_tag="ONT85_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HCR2378795.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(11118..11810)
                     /locus_tag="ONT85_001827"
     CDS             complement(11118..11810)
                     /locus_tag="ONT85_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HCR2378796.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(11832..12806)
                     /locus_tag="ONT85_001828"
     CDS             complement(11832..12806)
                     /locus_tag="ONT85_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCR2378797.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            13106..13978
                     /locus_tag="ONT85_001829"
     CDS             13106..13978
                     /locus_tag="ONT85_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCR2378798.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(14560..16758)
                     /locus_tag="ONT85_001830"
     CDS             complement(14560..16758)
                     /locus_tag="ONT85_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR2378799.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEVEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLFSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            16990..17982
                     /locus_tag="ONT85_001831"
     CDS             16990..17982
                     /locus_tag="ONT85_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR2378800.1"
                     /translation="MVTIKDIAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNLAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            18042..19463
                     /locus_tag="ONT85_001832"
     CDS             18042..19463
                     /locus_tag="ONT85_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HCR2378801.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSFPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            19456..20955
                     /locus_tag="ONT85_001833"
     CDS             19456..20955
                     /locus_tag="ONT85_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HCR2378802.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            20994..22388
                     /gene="uxaC"
                     /locus_tag="ONT85_001834"
     CDS             20994..22388
                     /gene="uxaC"
                     /locus_tag="ONT85_001834"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HCR2378803.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYLTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
BASE COUNT         6826 a         4579 c         3893 g         7181 t
ORIGIN      
        1 aaaagtgccg tcattacttt agttgaaaga ttatctaaag tcattatcac gattaaaccc
       61 aacggccgta aggcattaga tattgaaact gcccttaatc aatggttttc tcgcttccct
      121 aaaaacttct ttaaatctat tacgtttgac tgtggaaaag aattttctaa ctggaaagcc
      181 attagtaacc aacatgatat tgatatatat tttgcggacc ctggaacacc ttctcaacgc
      241 ccattaaacg agaattctaa cgggattctg cgtcgtaatg gactgccgaa atcaatggat
      301 tttagaaaag tgaatcagac atttatttcc agtgtcagca atcaacgtaa tcatattcca
      361 agaaaatcat tgaattacag aacaccaatc gaaatatttt tgagctatgt acaagaagca
      421 ttttattcta gcttaatttg acaaatcata tatcattaaa aaaaataaag gtcaagctaa
      481 aatattattt aagcttgacc ttattataat ttcaacgatt gtcaaaagta ttgtttgcag
      541 ctgtgttgcc agtttctata gataaatcac agcttttgaa acataagttt tgtactgctt
      601 cgattcgccc gaattctttt gcagttattt ggcaatgaga aaagcagaca tcttctatag
      661 gcttttcagg aaatgctact aaatcaaaag ctcgggaagg aagtgagtaa cctggagaaa
      721 ggcatgcctg tacgtttttg atgtgtatgt ctttgacttt cgtcatttgc tttgattcag
      781 gtatttcttc agccactgct tgccaacttt ctggcatttg agcaaggtca ttgctatttt
      841 ttctattgta ttcattatgc cagtccatta tccaagaaaa aggaaattgt acatcaatca
      901 tattcagatt ttctaccctg atattttcga tcactccccc tctttctttt gaagatttca
      961 ttcgaatacc acagtctgac tgaaaaaaat tgatatcgtg aatatatacg tcactaactc
     1021 cagcactaac ttcgctacca atagttacac cgtatcctga acggatttta catctagcaa
     1081 cctcgattct tgatgatttt cgattgactc ttgcaccatt gccatctctg ccagacttaa
     1141 tagcaataca atcatcacca caggccaaat cacattcata gacacgcaca tttgttgaag
     1201 aatcaatatc aatcccatct gtacttggtc catcattgtt gcggatgatt actttttcta
     1261 ctaatacatc gttagaataa gtgatttgca agttccaaaa acctgatttc tgaaaaatca
     1321 aatcacgaac aacaacatgt tctgcatgat acagtaaaca agcgcgtgga cgtttaatag
     1381 cataatccgc aatccaacga agtccctttt gatcgtattc agctcttgtc ccgctttttt
     1441 gatcttttcc ccaatacagc tcccaccaat ggtctccttg accgtcgatt attccttttc
     1501 cttctataag aatatctttc gccgagagaa cattcaaaat cgctgctggc catttcattt
     1561 ctacgccggc tactcgtgta tcgatttctg gaaatgcttc gatatctgtt ctccccttga
     1621 ggactgcacc ttcttcaaag ataagatgac tcttatcttt taaaaaaagg gctccgacta
     1681 aataaatgcc ttttggaata aatacaggta agtgttcttt cgcaccttga tcgattgctt
     1741 tttgcagtat tattgtatca tcttgttgag tgtcgtttgt tttaaagttc aaagcattga
     1801 taatcccttt ttttgtccgc aatacgtttc tccctatctt tcattgattt ttttcaattc
     1861 ttcaccaata aatacagttt ttgaacgatt cagtgggtaa cctactagca aaatgattgc
     1921 ggatacaaat aagcagaatg ctggtaacaa gtttgccatc gtataaatcc tttcttgtac
     1981 taaaactgat tggatggctc cacctgttgt agacgattgg taaccaatga gtgccagcat
     2041 aaatccgcct accgctcctg cacatgcttg tcctactttt ctcgcaaaag aatttactcc
     2101 atatactgtt ccatcttctc ttaagcctgt cttatattga tgataatcaa tcacgtcagt
     2161 aatgaatgcc cagaccatca aattgaataa accagatcct aaagtagcga taaataaaag
     2221 aatcagatat acccatgcat ttgttatatg aaggaaatac atcagcaagt acatacatga
     2281 agaagcaagt aacgcaaata cgcttgcttc ctttttacca aataattttg tcagctttgt
     2341 agcaaatggt gctaaaacta ctactgttcc gtaagtaaat aaaagagcaa cagacattgc
     2401 ttgtttatta tggaagtaat cattgaataa ataagtcata ttcgtcccag ctaaaatctg
     2461 attgatcaca atgaaaatgt caacaatgac taaaataatc agtgctcgat tagctaacaa
     2521 accttttacc aactgagaag cagatacttt ttcgtttttg acaactctta ctctttcagt
     2581 agtcaattta caagttaaca tatatgcgac aaatgctatt gcagcacatc cgattgaaat
     2641 ccaaaagaaa tgattccctg aaacgacttg ttgtccactc ttgttatggg tatacattaa
     2701 aatcggaatg aagaatcctg tagttgatcc gccaatcgca gaaccaatgc ttcggaacgt
     2761 ggacaacgag gtgcggtcat taggatttga gctaattgct gctgccattg ctccatacgg
     2821 gatattgatt gttgagagaa aaatcccata aacaatgtat gaaacaaaaa tataagcaat
     2881 cttcaccgtc attggaagat gttggacgaa tggcaaaaac aaaatgacag tcactaagca
     2941 aaatggatac ttcatttttc tgatccaagg aataaatcgg ccagtttctg ttaactctga
     3001 gacatcacat aatcttccaa ccatcacatc agcaaacgca tccaaaattc tcgaaatcaa
     3061 gaataacgta cctacgattg cccctgaaac gccaagaaca ttcgtataat aaatcatcaa
     3121 gaaactattg actaaaccta aaataaaaca gttacctaga tcaccgaaca tgtagcctat
     3181 cttatcggaa atcccaaatt cctttttttc tttttttact gtatttgctg taacagcatt
     3241 ttgtgaaagc atttgaattc ctcctacctc ttctagtaaa cgttttcaaa attataactc
     3301 tttttttctt gtttttatat tcacaatgtt ggctttttat tgcattatct tgacagataa
     3361 aaaacaaaaa ataatctaaa aacattgatg cataagattg ataacttttt ttacataaca
     3421 atatatacaa acaaaaaaat tcactaacta gtgaaaataa gttagtgaac ctcatttatt
     3481 aaagaattaa attacagatg atcttttcca tcatacttga aaaactcaaa atcagcataa
     3541 cttcctcttt ttttatttag atcatgcact gctatcccga taaaatttcc agtaaaccct
     3601 cctgagagta tacacatgtt tttttgcgga cctactttct tccattcttc agttgatacg
     3661 tctttcaaat gaaactgtgc tgtctctttc tttacagtga ctttcaaaaa agtcttcttt
     3721 tctgtgagtg gaatcgtcca atcttctatt tgtgctactc cgttttctga ttctatcata
     3781 cgtaggcatt ttcccttgtc ctcattccaa gtaacataac aataaatata attggattca
     3841 tttaagtaca gtaccagtcc agccatctga ttaaaattat taggcatgta ttcaaatgct
     3901 gtctccgcat caaaagtaaa atccgtttgc cgaatagcta acaaatgatg ttcgaaggta
     3961 gattgaagag attcaccgga aatcagacgt aaaaaacctg gtctacattt tgtatcacac
     4021 caatcagcat ttggcaaaat gcgtctggca ttccagttct tctttagctc tccttcaaat
     4081 gtatccataa actgatgatc tttttcttgt tgtacctctt catttgtctc aatcattatt
     4141 tcttccatcg gagttgtctg gctatcttta agtcttaacc aatcgtcgga cgtccaatac
     4201 acttcttgaa tagcggtttc tcttcccaaa atagccgcac cgtttaatgg tctggtacaa
     4261 agatatgtca tgtaccatct tcctgacggg gtttgtacta agctcccatg tcctgtacat
     4321 tgcaatgtag aagcaggttt gtcactagct gtcagcatag gaaaaccagg atcaagctcg
     4381 taaggaccag taatattctt tgctcggcaa actgtaactg aatgtcctct tcctgtaccg
     4441 ccttctgccg tcatcaaata ataatacccg ttacgaaaat acaaatgtgg tgcctccgtt
     4501 ttcgctaatt ctgtacctgc aaatatttta tagatgggac catcaagctt cttcttctct
     4561 gcatcatatt cttgtaatac gataccgctg gatttattcg gtgtgtccag acgataatcc
     4621 catagttcat tgacaagcca tttttttccc gtttcaagat catggaacaa agaaggatca
     4681 aagccgttac tatttaaata aattggttcg gaccaaggac ctgtgatctc agatgcagtg
     4741 atcaaataat tgtggacatc tttaaacgga cggcctgcac ttttgacatc tgtgtaaaca
     4801 agataaaaca atccatcagc ataactgatc tgtggcgccc atatacttcc atttgtagga
     4861 tttccttgca aagatacttg agtggtcaaa atatccgttg tatgtgacca gttgactaaa
     4921 tttttcgact catatactcg gattcctgga agccattcaa aagaggagac aataatgtaa
     4981 taccgctctt tcactcttaa tatattagga tctggattga atcctcttaa aataggattt
     5041 ttcactttga tagtctgcat gattcaggct ccttttctca ttcttactcg atataaactg
     5101 aatgccccag ctgctaaaaa agcaagtgaa ataaacaaca caacggcaaa cagaccagtc
     5161 atgtcctgta ataatccaaa tgtattttct aaagacaaag ttgaatgatc tacagcaagc
     5221 gactgataac tttttttaaa ctctgacgca gacatattac ttaaagtaac tgctaacccg
     5281 ccaaagaaaa tcaaagatag actgccaccg accattccta gcaatagttc ccattttctt
     5341 ttcatttctg aaccccctgc ttttctaaat gtatccattc aggtcttttg atcgatgttt
     5401 cataattgtt attacgttca gagaatttga cagtctgttc atttttcttg tcatcccaat
     5461 tatcccaacc agttgggtga acatgcccac ctatcttaca cgcttcaaat aagacatttg
     5521 catacggacg ccacggtctt cctaaataga cgtttctagt atcaggttct gcagtcaagg
     5581 tacagctttc aaaacgatac cctgtttttt gatttttcgg cgtagatgct gctgtgatgt
     5641 atccaggatt tttactggct aagctatgga tatgacactg atgaaaaacg gcatctgccc
     5701 caccaaatat gaaatccacc gttccttcaa tccagcaata ttcataggtc tgacgacaat
     5761 actcaggatt ggcagaagcc ggtgtacaaa aaggagtacc atctttttgc aaatcaggca
     5821 acggacccgt gcataaggta tcctgataac cagatagtcg acagtttttt aaagatacgc
     5881 gatctcctga attgaataaa gcaacagcct gtcctacctt ttctcctgga cctgctgtat
     5941 tttcgatgtg aagattttct aatacgacat tcgatccttc taagaaaaaa gtagcacttc
     6001 ggaaagtccc taactggcta ccgtcaagat actcctgttt tgcaaaacga ttaccggaga
     6061 taactactgt tcctgctccc acaagtttaa aattggataa tctgctttct atatattcat
     6121 catatcttcc ttcttttact atcaagacct ttggatggct atccggcagg cctgccaaat
     6181 aatcgattcc ttcttggata gtttcaaaat ccgtcggggt atctatagca ttctttccaa
     6241 ttatcagttt gtctttcatc ctcataaatg ctcccttatg aattgtttcc aaatatttct
     6301 catttcttgt gtttccatat gaccgcctgt taaagaaaaa gaagaaaagt tttctggact
     6361 acccattgat tgatactttt tgtccagccc ttttctcagc ttcagtacgc cttcgtccaa
     6421 gcacatcgta tcgttttttc caactaagct aagacgaggg cgcggagcga ttttttcttg
     6481 gattgctaac gttgtataag ctgtcaatag atttggcaca tagtaataaa acccatgatg
     6541 atctaggcgg cgatgtagca aaagtgtttc gatgtccacc tgtgccgcta gatcaataca
     6601 aactttgaca cgagaatcca atgctgtcac ccaccagctt aataagcctc ccatagaaag
     6661 accaatcgtt gcgagccgtt gagtatctgt gtcttccctt gtctccaaat aatcgatcaa
     6721 gctgatcaca tcataaatac gcattcccca taacgttttt cctgttaata aaaattgttt
     6781 gaacaactcg ctttcagaaa tacctccgcg ttcttcaaat ccccaagcat caatcgacca
     6841 aacagcatat cccattcctg tcagtacttc tgcaaaagat ggagaagcta agtaaggagc
     6901 accatggatc aattcacttt tcccgactga aaaatctccg ccgtgagaat gcaggtaaag
     6961 gaccgtcgga aacggtcctt ctttttctgg ataagcatag agggctggta ctgtttccaa
     7021 ttcatttaag tacaattgat agtattcaag cataaaaccg ttttgttttt ctcggtataa
     7081 tcgttttcca ctaggggtat gtctgctaga tggagcacct aaaaaatcat tcaactgcat
     7141 aagcaaagtt tttacgaaca accagttcat ccatattttc tgattgattt gattggaatt
     7201 tctcaatact gatttcttta gtattttcta atagaaatgc tggtccctcg tgattttcaa
     7261 tcgacatctg gttaaaggaa accttacccg caaagccaat ataaaatcct tgtctgctca
     7321 tttcctctat tcctgtcatc atagcaggaa caccgggaac tgcatcttgg gccatcgaga
     7381 catcgatatt gtcaaatgtg atttcttgta tgaattgttc cgctaatcca taaataaaac
     7441 ctgcagaagc atggacattc cttgccgtga tattcgaaaa atggatcctt ctgaatgctg
     7501 atgtccgttc atctatcgga taagcctttt tttcccatac atacggttct ttccctcgcg
     7561 gaccacagaa ataataaaga ttcagtataa atggacacat cacattatcc atgactatat
     7621 tggatactcg aatatcttca acgatcccgc ctcgacctcg tctagatttc aatcgtattc
     7681 ctcgatcagt ttcttggaag atacaatttg aaatggtaat attacgaata ctaccactca
     7741 tttcacttcc taatacgaca ccgccatgtc catggaccat cgtgcaattt gtgatcgtga
     7801 tattttcgca agcgatcctt tcgtacgtat cttcagtccc tgccttgatt gcaatacagt
     7861 catctcccac gtcaatatgg caattactaa tacgcacatt tttgcatgat tctggatcaa
     7921 ttccgtctgt atttggtgaa tccgctgggt tcaagatcgt caagttatca aacgttgcat
     7981 tactgcaaag aatcgggttg atcgtccaac ttggtgactg gatcaattta atatctttca
     8041 cagtaattcg atgacagtta tggaaactca tcaatttagg acgaggataa gcgagattat
     8101 ctggttcatt tctaaaagta tgccaccatt tttttccatt tccatctaat gtaccgaaac
     8161 cagtgacaga gatgttctct acattttgag catagataca ggaagcataa acttttcgat
     8221 ggactccttc ccaacgtgaa tgaacaacag gataatcttt tggatcgtca gagaatttca
     8281 gaaccgctcc tgcgcttaaa tgaagttcga cattgctttt taaaaataaa gctcctgtta
     8341 gaaattcacc tgctggaaca actacggtcc cgcctccgtc acttgctgca gcatcaatgg
     8401 cttgttgtat tgcttcagta ttcaattcat caattgatgc gccaaatgtc aaaatatcat
     8461 acatatttat aatgcctcca cttcaatcaa tgcttgcaag aatgcaccaa cgcctttttg
     8521 atcattacaa ataattggtt cactgatgta gtaagcatat gaaccatctc gctgatctgc
     8581 tccgcctaac cctgctactt gacagttttt attcaagttc agccagcctt cttttgtttc
     8641 cagaacaaat tcatcaagta atccttgatg tgatttcttc acttgtggaa gccattcttc
     8701 tttgttcagc agtcccaaac gtattccttt agccattgcg taaacaaaca tactactgcc
     8761 ggaagcttcc agataatttc ctttccgttt tgtttctgtt gttacttgat accatgtccc
     8821 gctgttttct tcttgatatg gaagtaatgc agccagtgtc ttttctagaa tttctttcag
     8881 caaagaacaa tctgtttctt catgaagtaa ttcataggta tctgctgcag ccatcaaata
     8941 ccagccaatc gatcggctcc aaaaatgatg ggatagacct gtttttggat cagcccatgg
     9001 ttggaccttc ttttcatccc atgcatgaat cagcaaattt gtttttggat cgaatagatg
     9061 tttataactg atttcaaact gtctcaaaac atcttggtaa tctttttctt cactaaaatt
     9121 ctggataaat tcagcataaa aaggagaacc catatataag ccatccaacc aaatttgata
     9181 aggataaatt tctttatgcc aaaaagctcc ttcggatgtg cgtggatggt ttgccaactg
     9241 ctgggaaagg agttctgctg cttttttata tttttcttcg cctgtttctt tgtacaataa
     9301 aaagaaaagc tttcctgtat ttacatggtc gatattgtat tcttcaacgc tgtagccacg
     9361 gatcgttcca tcttcttgca cgaaatagtc gatattgtct tttatgaaat caaaataaat
     9421 tttttcttgg gttcgttccc aaagtaattg gaacccttta agtacgacgc catagtcata
     9481 agaccatttt cctttatacc acttttcttc atataagcga ggtgttcttt ccatgatact
     9541 ttttgctaat ttctttgacc agttttccat tatttgctct cctttaaaaa ttaaaatagg
     9601 atttgggaca aataccaaga agtatttgtc ccaatcgtct atattactgt gccgctgctt
     9661 tgtatgcttc ctcgtactct tgtgtgattc gatcgcctcc ttgtgctttc cagtcttcta
     9721 cagctttttt gaatccatct tcgtcaagct gtcccatgat gtattgcacc gtagcatcaa
     9781 tgacgatttt ttcaatttct gatcctactt cattgttcgt cggtgaatcc aatggcacag
     9841 aaggatccat tacagcaaat tcttcatttt ctttgatcaa ttgattagag aattcttttt
     9901 cagggtctgt atctttgaaa gagaacgtca cttcactagg acgagagctt gataacggtt
     9961 gcacctcttg ttgccaaaga tccatatcga taaattcgat agaaccatca tcattcaatt
    10021 cataatgcgt tccttcgata ccgccagtca tcaatttata cacatcttct tctaaaagat
    10081 catcaacaaa ttgaagtaat ttcttcaatt gtgcttcgtc ttttacttga gatttgggaa
    10141 atgctagcaa gccgccaata ccatttcctt ctgaccaaac atgataaccg ccgccttcac
    10201 catctgagat cttgtttaca ggcactaatt ccatatcatc ttgaatacct tgacccatcg
    10261 tcttcagatt cttgatatcc accattccag tgtagattcc agttcttcct tgtgcaaatt
    10321 gttcttgctg atcagtcttt tgggtaactg caaagtcttg tgccaaatag ccattctcaa
    10381 atagatctct tgaaaaattc aaagctttca tatactcttc tgtatcaaat tctggagtga
    10441 atttgccatt atcgtctaca gcccagccat ttggtgctcc aaaataagaa gtgaaaagtt
    10501 taaagctggt ataacgaata tcactaggcg cacgatcgcc aaaaccaact gtgtcgtcca
    10561 caccatttcc atcaggatcg ttttctgtaa attgtttagc tacttcataa agctcatcca
    10621 tggtagttgg aacatctagt cctagattat ccaaccagtc ttttcgaatg actaagccag
    10681 cacgagcgta gtctttttgt atcggtacgc cgtacaaggc accgtcgata ctcgcagctt
    10741 cgattctatc ttcagaaatt tgacttaaat ttttatagtc ttttaaatat tctgatacat
    10801 cccagaatac gcctgatttt aacgcattgc ggacagaaga gtttttcaac atcgcaattt
    10861 gaagtgtaac gatatctcct agttcatcag aagccaatgc agttgttaat ctttcttctt
    10921 ttgaagcatc aggaatccag ttgaatgtca gttccgtatc ggttttttct tcgatcaagt
    10981 tcacaatcgt gtctgtaggc ggtgaagcat gatgcaaaat atttaaccaa gtgattttag
    11041 cttttccatc ttctgtctct gaagaactgt tacttgaact gctgccgcat gcggcaagtg
    11101 ctagaattcc tgcaaaacta agacctaatt ttacgagcgt tgcttttttc ataaattttc
    11161 tccttcactg ttttaataat atttttgttt tcttctgttt tttctgtata tgagttcttc
    11221 cattccaaat ggcaaaatat agaatttgct gtccacgtga ctgcggaaaa gaagagactg
    11281 ccaccgataa aggttaaaac acctggcatt gtttggatga tcatcagata aatccctacc
    11341 atacttaata tcaacactaa tgagtaatga agatgtgaga atccgaataa aaatgccatt
    11401 ttcattttga aaaaatatcc tttccaatca taatgagcca tgattgggaa aatatagagt
    11461 gctgagagca catatatgaa cgcagcaata tacaaagcga tcctaagata ccaattgtgt
    11521 atgaaagaaa gatccacaag taaaacaatc ccgatggcgc catatatcca gctcatcagc
    11581 aaggtttcac gatagttttc tttaaaatat gtgaagtacg ttttcgtcaa aggaaacgtt
    11641 tcttttgagc gcagccattt tctaatcact gcaaccatcg catacgatgc aggaccgaaa
    11701 gtaaaaactc ccaaacctgc gatgactaaa ccgccccaca gaaaattcaa ataaaccaac
    11761 tgtaaaatac gatttagcct tccgtttatt ttatctaatg actgcagcat atctttccct
    11821 ctctttctat cttagtaaac gccgtcttct cctagctttt tggcaatctt attagctaaa
    11881 attaccataa acaatcctac gaccccttta aacaatccca ttgctgtact gaaacttaac
    11941 tgtccattct tcaatccagc tgtataaata taggtgtcga aaatttctgc aacgcttcga
    12001 ttcaatgaat tcaataacaa gtacatatgc tcgaacccta agtctaatgt ctgaccgatt
    12061 tttagaatca gcaaggtaat gattactgga cgaattgccg gcaatgtaac atgccatgtt
    12121 ttgcgtaagc gtcctgctcc atccatttct gcagcttcat acaactgtgt atcgactgca
    12181 gttatcgctg aaaggtagat gatcgtagac cagcccaact ctttccagat cacttgtccg
    12241 atatatactg tgcgtgtcca ttcaggggat gttagaaaac tgattttttc aaatcccatt
    12301 gttgcaagga attcattcaa tacccctccg tcaacattca aaaatacata agtaatcgaa
    12361 acaatgatta cccaagacat gaagtgaggg atgtaaatga tcgtttgaat cccgtttttc
    12421 aatcgtttgt tcgttacttc atttaatagt aaagacaaaa tgattggcat cggaaaaaac
    12481 acgaagatat ttaatccgaa caagatcaac gtatttctta ataatgtaaa gaatgtcggt
    12541 tctgtaaaca aacgaatgaa atgtttgaaa ccgacccaag gacttcccat tattcctaaa
    12601 aatggttgat aatcttgaaa agcaataacg agtcccccca taggaagata cctaaaaatc
    12661 aagaaataaa ggactcccgg cagaatcatc acatataaaa atttattcgt tttgattcct
    12721 gcaattattt tttttcgtct tctgagcttt tcttcttttt tcgatagcgt cagttgaaat
    12781 ggttcttgtt tctttacact ttccataagc ccctccttag ttttgtgaat gtttgctgct
    12841 tatgacttca tgatagataa ctatctgaat tacgtatata atcattttta tcgtaagtaa
    12901 aaacgcttac atagcaatga tttttgtgct atacaaaacg ataaagtgta ttttttcaca
    12961 aataaactac gcatagcaaa ccttctattt ttttctatct gacacttagc ataggaaatg
    13021 attatatacg taatctttca ccgatgataa attgaacata tagaaacaga aacatttaat
    13081 ttattttgag gaggagccta tctatatgaa aaagaaagtc tctataggag aaagaagttt
    13141 tacacttttc aacaatacgt ttctaatttt gctagctttg atctgtatcg ttcctttttt
    13201 aaatatcatc gctacatctt ttgcttctac acaagaagtc gtcgcgaaaa agtttatcct
    13261 ttttcctact accttttctt tagacgcgta tcgctacatt ctttctacgc cgaccatttt
    13321 tagagcactt gctgtttcga ttggtgttac aggtgtcgga acgatcgtca gtatgtgtgc
    13381 aacttcactg atggcctacg gtctttccag aaagtatttg tttggtcgag gtttcgtcaa
    13441 ctttcttgtt gtattttcta tgttgttcag cggggggatg attccaacgt tcttagttgt
    13501 tcgttctcta gggctggtca attcttactg ggccatgatc ctgcctgtgg ctgttaatgc
    13561 aatgaatatg atcattatga gaaacttttt ccaagccttg ccggatagtt tggaagaatc
    13621 agcaaaaatg gatggttgca cagattttgg tgtatttttc aaaattatgc tgccattagc
    13681 tttaccgtct attgctacta tctcgctttt ttatgctgtt acttactgga acacttatat
    13741 gactgctatt ttgtatatca acgattcatc taaatggccg atacagattc ttttacgcca
    13801 aatcgtcatt gtatcaagtg ggatgcaagc agaaagtagt gccgttgatg tgattccacc
    13861 agcacaaaca gttaaaatgg ctgttatcgt tattgcaaca gtgcctatgc tgattgttta
    13921 tccttttgtt cagaaatatt ttgtcaaagg agcactcgta ggttctgtca aagggtgatt
    13981 atgtactgaa cgcaaaatgg tagttgtgtc agaagtggtc agcttcaaga aataagaagg
    14041 catttacgaa aattgcttct taaatttttg tgaaatttcg gcttattttt cgaagaatct
    14101 actcctggaa caccgtgtat cagaggttgt gaaagagacg ttcagccttg agcgatgata
    14161 aaaactctca caaacattgc gacgagtaac ccacaggagc aataagaagc gattttgaaa
    14221 aatagcttcc catatttcac aaaattatgc cttattgtcg atagcctgtc tcttgaacgc
    14281 catttattcg taaaaaagaa ctgtgacaag acttttgtca cagttctttt tgcgtgctta
    14341 gccttgaaat ttaactatcc ataaatacaa atactagggt tattctaact tcttttattg
    14401 cttgaatcag tccacaagaa aaacagcctc ttgattaaac ggcgtttatc ttgttgattc
    14461 tgcggcaata aaatcagcca gcagcaaaac atgaagtgct gttttttgct gtcctctttt
    14521 attgcttgaa tcagatcaca agaaaaacag cctcttgatt cacaaatatt tttttcgata
    14581 ttctcccggc gtaatcgatt ccttcttttt aaagaaacga ataaaatttt gcggatttcg
    14641 atattgcaat ctttctgcaa tctcttttac ggagaggtta gtctctttca accatttttt
    14701 tgctacctct aaacgatgat tttgaacaaa atcaccaaat ttttctcctg tttccttttt
    14761 aaatacatta cttaaatagt tagggttata atgcaaccta tcagcgatca catctaaaga
    14821 cagatcacga tcatattctg tgtagacgat atgcataata ttctccgaca aatttttaaa
    14881 ttcatgatca gtgattgctt gagagcattc tgtgattggc agaactaatt gttctaaaac
    14941 aagtttttct agttctttgg gatgataatt gttcagtgct tttacataaa tttgtttcat
    15001 gttatcaaag ttttttgaat ctactcccaa taactgtcct aattgaacga gttcattgat
    15061 caagcggacc gaaactactt cccgactcaa aggattttta ttttgtgcaa acaatgcatc
    15121 aatcaattca tgtactaaat tcttcacttc ctgatttcca gaacgaatgg cttcaaacaa
    15181 tcgattttgt tcttctatcg gatatctaat aagtgttttt tcatgagcag caggaacgat
    15241 ttcctgataa aaaataattg aacttggacc agtatttaca cgataataca gggcttcttt
    15301 tgcccgatca accgattgct tgctttcatt cagcgtttcg aaccggttac tgatgcctat
    15361 actgatagtt aaattcaagt attcttttac acgtttttga atgagtcggc aatactccat
    15421 gacttttttg cttgcttctg catcatcttt atccattcga taaatagtgg cttgcatttc
    15481 ttcattcaaa acgatcggca gcattcgatt ttcctttggc acaatttccg tgatcatgtt
    15541 gttgatcgct aaaagtagca aatcacgctc cccaccgtga ctatcttcta acagatcgat
    15601 ttgaaccaaa gctgtataat agcagtcatt ttgagaacta taaccaaatt gctgcaaccg
    15661 ctgatctagt tctcgccttt ctactcgatt tctaaataaa ctaagtacaa atagcgtttc
    15721 cagctgtggc ttttgggtaa ctaagtgtgc acttaacgct tctttttctc ctatgatccg
    15781 actgactgat tgagcgacca aatctagttc tttcccactg aatccttttg atttaagatt
    15841 cagacgttcc tgtatttgag aaatcggacg ggcaaatctg tccgaaaata agtaggaaaa
    15901 aagaccaatg agtcctacca gtgttaaaga cactgcaact agcccgattc tcagattgcg
    15961 gatcattgcg ccaattgcac tagagtcaac ttctaatctg taaatccaat tattataatc
    16021 tgatttcagt actatatacg ttttatcttt ttctcttatc actttcagct gattgtaatc
    16081 tagatccacg tccgttaata cagttggttg ctctttattt tttactgtct gagtagtaaa
    16141 aaggttgcca acttcgtttt caatagaaag tatgtcttct ccactttcac caacaacttg
    16201 ttctattgta tgtttctcta tcgtagcaat tcccaatgca tatttttctt tttcaaacat
    16261 tggcaaagaa atcaccattt cgattccttt gtttttcttt tgccaaaata aattattttt
    16321 gttatttatg tacttctgtt gatacttttc aacttcctct tcggtcagtt gactcaacga
    16381 tccattgatt actccccatt taccagccaa actgactaaa tcataattat tatcatccat
    16441 aacaatcgtt tctataaaat ttagttcttt cctgatttca gaataagtgg agtaatctgt
    16501 atatgtcagc ggatgcttga atatgtccga gaaatttttg gtcgaaccat acgtagaaaa
    16561 cgaatattca atcgttctaa ttttccattc aacgttagtt ttaacttgcc tgagcaaatt
    16621 ctttttgtct gttaaataac tcgattcgat cgattcatag gtattctggt aaatgaataa
    16681 actaaatcct atgacaagta cggctcctaa taaaaacatc ggtaaaaata ttttataaaa
    16741 tactgtactt ttcttcattt tcttcctctc ctccttttaa aataaaaggc tttcattttt
    16801 ctgtaaaatt gatactcatc taattaaatg agcattcccc gctttcaaga atagcataat
    16861 taaaaataaa aatccaagta atttttaacg tgtgcaatct tttcatatca aagatctatt
    16921 gacttatcgt agttatcgta atatgatgaa tgcgttaaca aaaagtgaaa aagtaaggaa
    16981 aatgatggaa tggtaacgat caaagatatc gctaaagcag ctggcgtttc tcatacgaca
    17041 gtttctcgtg ctttgaataa cagcagttta ataaaaaatg aaacaaaaca aaaaatacga
    17101 caactagcta aggaaatgaa ttattcgcca aactatagtg caaaaggttt agtgaatcaa
    17161 aaaaaatatc taatcggact atttttctca agtttgcata aaggaacttc atcaaatttc
    17221 ctagcagatt ccattgcagg catccattca gtattagaca caaattacag tttatctgtt
    17281 gaaagtatcg atgttgttga tttaacagaa atcaacctgc aacgctacga tggactgatc
    17341 gttatgagtc aaagtgatga tgatcagcct ttcatcgact atctcaaaga aaaagaattt
    17401 cctttcgttg ttgtcaatcg tcacatcaat gatccagcgg ttatcaacgt cactgccgat
    17461 gatgcggccg gtgttatgca ggcagtagat tacggtattg gatgcggaca tacgaagatc
    17521 gcttatattg gcggaaagaa aaattttcgt tcgacaaaag aacgggaaaa aggggtattg
    17581 aaaagtctaa aaaaagcgag catccctgtc aatctagcct attttttaac aggagattac
    17641 agcttagaaa gcggcctcca aaacatggaa tacctattat cactttctga cctgcctact
    17701 ctggtttttt gcggcaacga cgacatagcg attggtgcat tgagagctgt acatttagct
    17761 ggcctccgtg ttccccagaa catttctctg atcgggttcg atgattctcc agtcgtctcc
    17821 tatctcaatc cgccgcttac caccgtacgt aagcctttga aagaaatgtg tcaagaagga
    17881 acaaagcttt tactagattt gatcgaaggc aggacgatcg aacaatctaa aattgagctc
    17941 tccaccttgt tgatccttcg tgagtcgatc aattacttgt aaactacaag taattttata
    18001 taaaaatgtt aacgtgtgca taacacagaa aggaagttta tatgaaaaaa ctgacaaaag
    18061 aattagtgaa aaaaaacgaa gcacctgtaa aagttcttca gtttggtgaa ggcaatttta
    18121 tgcgtggatt tattgattgg cagattcagc agctgaataa ccaacaattg tttaaaggta
    18181 atgttgcaat cattcaacct ttagaacaag gtcttggaaa tatgatgaac cagcaagata
    18241 atttatatac ggtcatttta caaggactca gcaatggcga aatcgttgat acttccgaga
    18301 ttatcaccgt agtagaacaa atgatcaatc cttatgatga atgggatgct tatctatctc
    18361 tagcagaaaa tgacgacttg gcctatatcg tctccaatac aacagaggca ggaatccaat
    18421 tcgtggagac tgataattta acagatactc ccccacagag cttcccgggc aaactgacag
    18481 cctttttata ccgacgtttc aaattaaaca aagctggctt taccatcatt ccttgcgaac
    18541 tgattgacag aaacggcgaa cgtctaaaag aaatcatttt acgctacgca gaaaaatggg
    18601 atttagaacc tgcttttgtt gagtggttag aaaaagaaaa tgttttctgt tgtagtttag
    18661 ttgatcgcat cgtcccaggt tatcctcgcg aagatgcagg tgttttcaat caacggcata
    18721 actatgaaga tcaattgatg gtcaaagcag aaccattcat gctttgggtg atcgaagggc
    18781 ctaaagaatt ggagaatcaa ctaccattca aacaggctgg cttgaatgtc atcatcacag
    18841 atgatatgac tccttatcgt gaacgtaagg tgcatttgct taatggaccg catacagcga
    18901 tggtccctct tgctttgttg gcggaattgg aaactgtaga agaagtaatg aaagatcctt
    18961 tattctccgc atatgttgat cagttattca atctagaatt gattcctatg ctttctttac
    19021 ctaaagatga attagccatt tatgctgacc aaatcaaaga acgtttcttg aatccttttg
    19081 cccatcacaa attagaagcg atttcattaa atagtgtttc aaagttcagc acccgtctgc
    19141 ttccagtgtt caagaagtat atcgaagaac aaaatcaggt accaccgtta atcactgttt
    19201 cactagctgc gctacttctt atgtatcgag gcgatcaagt aaagccacat gatgatgaaa
    19261 agacgataag cgaatttact gatgcttggt ctgataatgg aacagctatt cctcgattat
    19321 tgcaaaacgc tgcgctttgg ggagaagatt tgtctcaaat accgaatgtg acagatacag
    19381 ttcaagaaat agctgcaaag atcgatcaaa aaggtgtccg gtcagtatta caagaaatga
    19441 aaggaacgat ggtccatgct tgaaaccctg caaccaacaa tgaaattaaa tcgacgagac
    19501 agtgtggtcg tcgcattaac ccctattcca aaacaaacac aactgacgat tgatcagcaa
    19561 acgatcacca ctcttgaaga tatcccgcaa ggtcataaga ttgcattggg tgatttgaaa
    19621 gctggagaca acgtgatcaa atatggctat ccaatcggtc atgtgacgac agacgtaact
    19681 gccggtcaat ggctgcacac tcataatgta aaaaccaatc tttctggtga attggattat
    19741 cgatatgaaa aagatgtcca tccaagtcat tacctttttg agaaccgaac atttcaaggt
    19801 tacttgcgaa aaaatggaaa agtagggatt cgtaatgatt tgtttttagt acctaccgtt
    19861 gggtgtgtga atggcattgc tgaactgatc gtcaaacagt tcaaggaaaa acatccagat
    19921 ctgggacagt ttgaccatat tacgatttta aagcatcctt atggctgttc gcagcttgga
    19981 aaagatcatc aaaatactcg agaaatttta gcggacgcag taaatcatcc caatgccgga
    20041 ggagttttag tatttggatt aggttgtgag aacaatacag ttcctgaatt caaaaaaatt
    20101 ttaggtgaat atgatgaaga acgagtgaaa tttctagtgg ctcaagatgt ttacgatgag
    20161 atcgaacaag gtgtggctct attagaagag cttttaacag cggcggaaaa tgaccagcgc
    20221 atatcagtcc cgctctctaa gttgaatgtc ggtttgaaat gcggcggttc tgatggtcta
    20281 tcgggaataa ctgctaatcc tttacttggt gcattttctg actacttgat tgctcaagga
    20341 ggcagcacga tcttaacaga agtgccagaa atgtttggcg cagaacaagt attgatggca
    20401 cgagcggaaa atgaagaagt ttttgactca atcgttgact tgatcaatga cttcaaacag
    20461 tatttcttgt cttacgggga accagtttac gaaaacccat ctcctggtaa taaagcaggt
    20521 gggatcacta cattagaaga taagtcttta ggctgcacgc aaaaatcagg aagctctcct
    20581 gtcgttgatg tgctacaata tggcgaaaaa atcagaaaac cagggcttag tttattgcaa
    20641 gctcccggga atgatcttgt agctgcctct gctcttgctt catccgattg ccagttggtc
    20701 ttgtttacca caggaagagg gactccgttt ggctcatatg tcccaacggt caaggtatct
    20761 acgaatacaa ctctttttga acgcaaaggt cattggatgg attttaatgc aggcgtctta
    20821 ttagaacaac caatggaagt aatcttagaa tcgtttgttc aaaaaatcat cgctgtagct
    20881 agtggagaag aaacgaaaaa tgaacaaaat gacgttcgag aaattgccat ttttaaaaac
    20941 ggagtaacac tttgattttc ataaaacagt ataaaaagaa agaaggaaga tttatgtttt
    21001 tatctgatga ttttttatta aaagacgatt gggcgaaaaa gctttaccat tcctacgcaa
    21061 aaaacatgcc cattattgat tatcactgtc acttagatcc aaaagaaatt tacgaaaaca
    21121 acaatttcag caatctgaca gaagcttggt taggaggcga tcattacaaa tggcgcttga
    21181 tgcgagcttg cggcgtgcct gaagaaaaaa tcacaggaaa tgcttctgat tttgaaaaat
    21241 ttttagcttg gtgtcaaacc gtaccaaaaa ttatcggaaa tccgctttac agttggtcac
    21301 atcttgaatt gaagcgtttc ttcggtattg acttgttgat aaatgaagaa aatgcagaga
    21361 aaatttggac acaagcaaat gaaattttgg cagctcctga ttttcgccgt cgggaaatcg
    21421 caaaaaaatc gaatgtagaa gtgatttgta caacagatga tcccgcagac gacttgcact
    21481 atcatcagtt gcttgctcat gaagaaccag atttgaaggt tctaccttca ttccgtccag
    21541 ataaagcact caatattgaa aaagaaggat ttaatgcatg ggtcacacta ctggaagaag
    21601 cagctgacca aacgatcacc acctatcaag aactgataga agtcttaggg cagcggatag
    21661 aatacttcca tcaacatgga tgtcgtgttt ccgaccacgc tttagacatc ttacgctata
    21721 aagaagcgga tgaaacagta ttagaagaga tttttcaaaa agcacgagct aacaaactat
    21781 tatcttcaga tgaaatcgat gcttatctta cagaaacact gatacatttg acccacttct
    21841 atcattctca taattggacg atgcagctgc acatccatgc ttatcgtaac tgtaatacac
    21901 aaatgttgga aaaattaggg cccgataccg gctatgatgg aatgaatgat ctttctttga
    21961 ctattcctct gcaaaaatta ttgaaccgtg cagaagaaac ggatcagttg ccaaagacga
    22021 tcctttattc attgaatccg aatgactatc ctgcgatttt agctttgatg ggctgtttcc
    22081 aaaaagaaca gaatggaaaa ctgcagttag gttctggatg gtggtacaac gatactcgag
    22141 ctggtatgcg cgatcaattg acacaatttg ctgatggtag tgctttgggg aattttgtcg
    22201 gcatgctgac agattcaaga agtttcctgt cctacactag acatgaatat ttccgtcgtg
    22261 ttctttgtga atttttaggc gaaatggtca tgcgaggaga agcacctgaa gatgaacaac
    22321 ttttaggaag tctagttcaa gcgattagtt atacaaatgc taaaaattac tttggctttt
    22381 ttgcctaatt gattaatagt aacagtaacg aatcaaaaac aggatagtga aataacggaa
    22441 tccgtttatc cacgttttag agaagaagac ggaccaaat
//
LOCUS       DAJSBL010000039        21988 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.039, whole genome shotgun sequence.
ACCESSION   DAJSBL010000039 DAJSBL010000000
VERSION     DAJSBL010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21988
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.039"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..395
                     /locus_tag="ONT85_001835"
     CDS             <1..395
                     /locus_tag="ONT85_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2378804.1"
                     /translation="ISLKDESNTTVSDANILANIILDFIFEHHIPFKEGYEILPANQE
                     YYFYKCITKRVCCICGKTGADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKI
                     GVINFKNKYQIKGIKLNQKTIKKLNIGG"
     gene            398..1147
                     /locus_tag="ONT85_001836"
     CDS             398..1147
                     /locus_tag="ONT85_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR2378805.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            1159..1428
                     /locus_tag="ONT85_001837"
     CDS             1159..1428
                     /locus_tag="ONT85_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2378806.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDTTQLRNLAYTKLLFVDCVKGLQKHLNELEQV"
     gene            1433..1597
                     /locus_tag="ONT85_001838"
     CDS             1433..1597
                     /locus_tag="ONT85_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2378807.1"
                     /translation="MDLKTFTAQIELMHQEALRQSVSYEDKWLNTFHGGRESALDQVL
                     KLLKGECRDG"
     gene            1590..1892
                     /locus_tag="ONT85_001839"
     CDS             1590..1892
                     /locus_tag="ONT85_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2378808.1"
                     /translation="MDKKAAMQRIIELTYSEDWQNDKEAASEVMRLGRAMWADKSNKP
                     RPRKIAIWHGDKLLVIGTAEQLASLTGLHEKIVRKRARCGYTDAKKRTFRYVEESS"
     gene            1889..2050
                     /locus_tag="ONT85_001840"
     CDS             1889..2050
                     /locus_tag="ONT85_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HCR2378809.1"
                     /translation="MTTEEVIQMRIRSLQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIHRLEE"
     gene            2047..2352
                     /locus_tag="ONT85_001841"
     CDS             2047..2352
                     /locus_tag="ONT85_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2378810.1"
                     /translation="MSVDKKAAMKRIAELTKSESWQEDKEIVAEVQKLGKSMWTEKPK
                     RRTPRKIAIWHDDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEER"
     gene            2352..2708
                     /locus_tag="ONT85_001842"
     CDS             2352..2708
                     /locus_tag="ONT85_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HCR2378811.1"
                     /translation="MDLITQYSDIILKKIMMKIQKDKKSKERAELVKLEMAETGAGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVEKYKEILEEYLR
                     RTANDKKTRSIQHGFI"
     gene            2695..2913
                     /locus_tag="ONT85_001843"
     CDS             2695..2913
                     /locus_tag="ONT85_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2378812.1"
                     /translation="MDLYDIESGATLSVESDHLIINFGGKRQIILWVVDDVLFPEIVH
                     DFEESKAVEFEIVKKVMELIEKYEEDSE"
     gene            2910..3329
                     /locus_tag="ONT85_001844"
     CDS             2910..3329
                     /locus_tag="ONT85_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YopX family protein"
                     /protein_id="HCR2378813.1"
                     /translation="MIPKFRAWDKRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIDLMQSTGMKDKNGVDIFEGDIVLVSVRNGFDYLDNKVCIVKNSIDYSGLVCAT
                     VDEDLEYQIFNTELFEEYTYEVTGNIYENSELLEEQR"
     gene            3326..3883
                     /locus_tag="ONT85_001845"
     CDS             3326..3883
                     /locus_tag="ONT85_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HCR2378814.1"
                     /translation="MNKQEAIKKLESIKAIGNDAIAACYNESINSGITLMKKIDEPQK
                     PVIPQLVAGWLEKSTDPFTKAEKIAYLIKSKDGDSYYFCDWFVRDGIVTQEQGEELLA
                     WATRQSYETLLSLYNGYEVEKEPLWAIKNADGNYLTKCALWGKDGVNYSFECNPSHRL
                     LFTDKATADAAALLVNGTVEEVVER"
     gene            3880..4176
                     /locus_tag="ONT85_001846"
     CDS             3880..4176
                     /locus_tag="ONT85_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2378815.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKSKFKVGDMVRCEA
                     EEFVYPFRGYVKKILSNSAIIRIENTMKCDRSTAKHKHYLAVARLVDMEVIKSK"
     gene            4253..4666
                     /locus_tag="ONT85_001847"
     CDS             4253..4666
                     /locus_tag="ONT85_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HCR2378816.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGRIIAYK"
     gene            5012..5386
                     /locus_tag="ONT85_001848"
     CDS             5012..5386
                     /locus_tag="ONT85_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2378817.1"
                     /translation="MEDKMYLRQKVFTKKDIENQINGFIQKLENANSYLINKELNKAY
                     DQWKEVYDELKLIQNETKLVRTEKKNENSFFFDGYAILMLETVAKQNIKAPKKELSDN
                     IDNALAELRYYVMQIKDVRITR"
     gene            5423..5590
                     /locus_tag="ONT85_001849"
     CDS             5423..5590
                     /locus_tag="ONT85_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2378818.1"
                     /translation="MYRPQYLEQKYEVITVQNGNGEIVRKYRRPIKSDTYKRKESNEV
                     IPLYGKRIAKH"
     gene            5616..5960
                     /locus_tag="ONT85_001850"
     CDS             5616..5960
                     /locus_tag="ONT85_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCR2378819.1"
                     /translation="MNEEFYRWLLQLIREDRLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTALDKQEKKI
                     ESFANFDASERW"
     gene            5965..6246
                     /locus_tag="ONT85_001851"
     CDS             5965..6246
                     /locus_tag="ONT85_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2378820.1"
                     /translation="MIINDNGREYDTEKIEEYSSYTQGLIKRLIYVRYVGIRDLLSDN
                     CCSKYKVNQVREALNKDNNVERIKNVFGYSIEEINYYIDFAEAFIPMVR"
     gene            6349..6663
                     /locus_tag="ONT85_001852"
     CDS             6349..6663
                     /locus_tag="ONT85_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HCR2378821.1"
                     /translation="MFKNELSQNRYREKLRRSLISQLESQKTNIEPFLDNVDRYISLW
                     ETAISLEEDISENGIRLENGKKNESVALLVSVNKQMGLMLDKLAITPELVGEANESIP
                     EL"
     gene            6641..8335
                     /locus_tag="ONT85_001853"
     CDS             6641..8335
                     /locus_tag="ONT85_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HCR2378822.1"
                     /translation="MNQFLSYKHIENWFKAIEEGTIKVCKEQLLLKNYLEERVFTRED
                     IYFDKQMVEDSINIPAQYFPFELIPWEKFLQCFIYGVRWKKDKTLVFNRYLSLMGRGN
                     GKTGFASWNNFFLLTAKHGIKNYDIDIYANNESQAKTSFDDVFKVIKDHPDLDKKVFK
                     ATKEVIQNIATNSKLRYNTANARTKDGKRPGANRFDEIHENEDYSMINVATSGGGKIR
                     DYREFYDTTNGHVRGGPLDDIIEESKMILSGELGIDKDGAEFSSLFPFICRLDNDNEV
                     DDPDMWEKACPTINYNANLKRKMFQEYSQMQRNAGLRLTFMTKRMNRPMEDTRFAVAS
                     YDDVLHTKEKEFPEKMDEVIGTVDFADRRDFASVGLLGKYDKDVYFTQHTFIHESALR
                     LQNIKREVIDISIDQGKSQIVHGKNIEADYIVGWFLEMSNKYYIKKIAMDMYRAKILK
                     PALEEAGFTVEIVRSGSVTHGMLKDLVDDLFINQRLFFGDDAIMRWYCMNVYEEHISN
                     GNIRYEKIEPETRKTDGFFSFLHGLNFLDDIYDSAPVTVTNSSVENTGTGFTPLVF"
     gene            8355..9533
                     /locus_tag="ONT85_001854"
     CDS             8355..9533
                     /locus_tag="ONT85_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HCR2378823.1"
                     /translation="MGIFQKAVGYFTKKATVPLEEYFCKLQVDFVYRKFAIETCIDLI
                     ANAMSKAEFKSYEDGKNKKNDLYYRLNVAPNKKNNATEFRKKLIRRLIFYNEVLIVSP
                     SNNSSEIFIADSWDVTEYALKDDVFSQVQINNIVLDREFLESDVIYIKYADQQIRQLV
                     DAYYQAYGKLISSAMNVYKRSNARRYVLKGNLFRPQDNTTQDQINKMMTSQFKAFMEA
                     DNAGAVFQLQNEYTLEDFSGNFQSNSRDIKNLIDDIFEMTAAAFHVPKNLLKGDMSGL
                     SDQVDAFLMFEIIPIAELIQDAFNASLYEVEEYLSGNFVRVDTTMIKITSFKDLVDAI
                     DVGIRNGVFTINEGRERVGNDRSDKAMADEIFITKNNQQVSKGGEANDDNENISSSKE
                     "
     gene            9496..10182
                     /locus_tag="ONT85_001855"
     CDS             9496..10182
                     /locus_tag="ONT85_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HCR2378824.1"
                     /translation="MTTMKTFLAVKNEGAVPQIFIQGFIGSSWFFEGNTDKGIKNILD
                     SLGDQEEIEVVINSNGGDVFQGIAIGNLLKSNKAKVNVVINGLAASAASIIAMAGDTI
                     KIYNNAQLMIHRASTYGEGNVDDFRTIADQLESIDKSVKASYKTRFNGTDEALQELLE
                     KESFMDAETALSYGLVDEIIDAENSSGTEAKKEQSVEEILNDVKEKRAEKIAAFTAAL
                     NKTFGQGDVK"
     gene            10182..11342
                     /locus_tag="ONT85_001856"
     CDS             10182..11342
                     /locus_tag="ONT85_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HCR2378825.1"
                     /translation="MTVKNLKGVTAASDQLMKAFKDGNEESFSAAMVSLSKEIQDKIL
                     EEATAKNQDQLVLMNRGQRVLTTQETKFYNEVVKNEGFAGVEELVPATVFERVFEDLE
                     QSHPLLQKITFVNTTGVTEWIVSRGVNPAWWGKLCEAVKKVLDNGFDVINMKQFKLSG
                     YIPVCKAMLDLGPVWLDRYVRTVLVESLRIALEQAIVDGTGKDMPVGMMRDMSKQTSG
                     EYAEKKAEPITALDAVTMGRLMARLSKFNIEGVNDPIYRNVNPSDVVLIVNPTDYWSK
                     VFPAKTVLTANGEYVQVLPVPVSDLQSTAVPEGKAVIGVASDYFMGVGSTLKIEASDE
                     YHFVEDERIYLAKQYANGQPKRNDSFIVLDISALGTTTTTTKPTTTTTTTQA"
     gene            11352..12227
                     /locus_tag="ONT85_001857"
     CDS             11352..12227
                     /locus_tag="ONT85_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2378826.1"
                     /translation="MKYILCQPAINRFKWELEVCLTNLKKLGIKDIVLLFSRHDDQIP
                     IFFEKEYGVEVHVYDDLRDDKEYIPSIKPYLWWKYLEEDHSREDDRYFYIDSDVIFNK
                     RINLRKLPSKDDVWYCSDCCSYLSLDYIRSCENGENILKDMANIVNVTVESLETINTN
                     SGGAQWVINRPKANYWKKVYLDSNRLYRYLRGQKTNIQIWTAEMWARLWNMMYFNIGP
                     KVHEELDFCFATDPIEKVKEVKILHNAGVTTNDEDLFFKGRYVTSTPFDEDLSFVNKK
                     KCSYAYVKAIKAVVR"
     gene            12224..12535
                     /locus_tag="ONT85_001858"
     CDS             12224..12535
                     /locus_tag="ONT85_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2378827.1"
                     /translation="MTPEQVTEELLIAVKDNIYVTWNEEDESIKKMIAKNAVYLQSKV
                     STTLSFSPESLEYGLLIERCRYDWNRALDEFEQNFASELLGFIQHYALQEYIAGDVNG
                     E"
     gene            12525..12878
                     /locus_tag="ONT85_001859"
     CDS             12525..12878
                     /locus_tag="ONT85_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HCR2378828.1"
                     /translation="MANNRRLEETFNDGWLKILTQTTKRNELGKKIGVEDTEITSLKF
                     RNLSMRDSDITAMDAMGSKLTKKVKTPFHPIAKKFNKDQYFIVIDSMRYNVIYADYDN
                     FYIYFYLESVGEYGD"
     gene            12868..13269
                     /locus_tag="ONT85_001860"
     CDS             12868..13269
                     /locus_tag="ONT85_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2378829.1"
                     /translation="MVIDNSKEKERLNKQISAIKTSLEEHFGLKLFQDSVGEDELPDD
                     FNYFILETGEIEMITEPKYSVGQNLYLTFYSENREDLTGDSLDIISLIQNRSIRFQRM
                     DPNHLKLENQDRYIDQLVFTFRRLLKSDCHG"
     gene            13262..13666
                     /locus_tag="ONT85_001861"
     CDS             13262..13666
                     /locus_tag="ONT85_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2378830.1"
                     /translation="MAKNSWELKINGHDELLVRMERYSSESERLINEALKSKGSAIAV
                     DRITEKIPVSEADLRRGHQHAKNSRPLKTQYINLGFIIRPTRKFEYLKYPDLGIGTSK
                     RNQPDEFMRRGLGLALDPITELLIRQFDKLNK"
     gene            13678..14286
                     /locus_tag="ONT85_001862"
     CDS             13678..14286
                     /locus_tag="ONT85_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR2378831.1"
                     /translation="MAKTKTVVTTFDNVSIKRIAFNFKNAGNAIATNCNGQLDGETEM
                     QTVVKKCGATEVKSKSKPINMTVTITAHVPMEVYRRFNGLKQDGRIKPGIYSYGPDSV
                     GEDFSLAAEIVDDFEENRKLVGMLACTSNTGLTFSIENGADEVAALELETKVMQDEFG
                     KFYHEAIVAELEEDLTDQWMTNLSADVIKKTSVVTTTATQSQ"
     gene            14306..14668
                     /locus_tag="ONT85_001863"
     CDS             14306..14668
                     /locus_tag="ONT85_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2378832.1"
                     /translation="MNEDYSKIELNDGTILNLEPKLNIKKLLMINRDFNTDEFAKMTV
                     GKGSMDISVIQGAKAVYIAYRQANMTDYISFDEFIDKWDFDMATASYIYQLMMFKQAR
                     DAYQKEFEKANKEKKLQK"
     gene            14671..14853
                     /locus_tag="ONT85_001864"
     CDS             14671..14853
                     /locus_tag="ONT85_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2378833.1"
                     /translation="MPKLLVETWVDVYSMLTDVFSMPSDLVLSDICLDDILQMAYNKS
                     AYEGWKNYAINQSQKN"
     gene            14870..18289
                     /locus_tag="ONT85_001865"
     CDS             14870..18289
                     /locus_tag="ONT85_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCR2378834.1"
                     /translation="MAKKRTEAEVTFIANDDGLKSTLKEISAELTKNRAELKLEQAQL
                     QQTGSESDKLGSKLSSLEKQYELQSQKVEVTSQRLANAKKYYGENSTEVQKLERELIN
                     QQTAQQRLSNEIDKTSNTLAQAKGEIQTYESTMQQLDSEQKNVQASASLIESEYKKWQ
                     VTAGQSASESEKLAKAQEYVSQQSENAEKTIDILRRQLEATQSEFGATSTEAMQMEAK
                     LNDAEREFEELGQAAKNVDTTNLDDIGSKIDMNNLMEASDVLSDIGDKLTELGKQAVD
                     SANSVGSSQSKIQANFGLTKQEAEELTNVARDIYYKGFGESLDQSTDALILVKRNLGD
                     LNNQDLQNITEQAMVLENTMGADMDETLRGVNGLMVNFGLSAQDAMDLMVSGTQNGLD
                     KTHELGDNMAEYSQLWSQMGYSADETFGMLQNGLDAGAYNLDKVNDLVKEMGISLTDG
                     RFEQNMDMFSESTRKAFEEWKNGGGTQKDVINSMIQDFSNMDGQYDQLNKASTIWSAL
                     GEDNAMKVVQSLTDVNHTFDDVSGSAQKMNEDSTTPLQELNGKIAELKDSLAPIGNTI
                     IDALEPVIDFLGKMADAFNNLPQPVQDYAVAIGGLTAAFTLLMPIIVGFMALGGPTTL
                     IIGAVITAIAGVIAIIKNWGAITDWFKGIWSKFTDWLGGTWESIKEGASSVWDGVKET
                     WSGFVDWVQEIWQGVSDWFGELWSGLVEGASNIWQGVQETWQAFVDWVSNIWNGVKEV
                     WSIIWADIVGIVQIPWTLITSLIQAGINIIVGIFDVAGQLLGAAWQAVWTPISDFLKN
                     TWDTMTQWISIAWNGIVTTFHTIFDPVVAWWNGIWTSISTTASNIWNSISATASSIWN
                     SIKNTITSLVQAAATVIQNIWSTVSSWLGGIWNSISSTASNIWSSVTSSISNAINAAK
                     SAIQSVWNSISSWISGIWNGIKNTALNLWNGITSTISSKVNDGKNAISSGWSNLTGIV
                     SDIFNNVKSTIANIWEGIKKTVSAPIDWIRDKISSVFDNLNISIPHIPLPHFKLSGEF
                     NPLKGKIPTLGVDWYAKGSVFNSPNIIGVGESGPEAVLPLKRSVLQEIGDRILSSTSV
                     SSRTQAVQPVNNYEFNFTIDGNADEVTMKQTTQQIIDSITKVQNDNASAWR"
     gene            18340..19077
                     /locus_tag="ONT85_001866"
     CDS             18340..19077
                     /locus_tag="ONT85_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR2378835.1"
                     /translation="MTDCIHSIIDGFPDYLHKLALAERPTIPSPKRQRVETSVLGRLG
                     GLVQDYSFEDMSFTLHYNYLEDVEDHQAFKQSFYIMRHWLNYAKKLEFSDDPNVYYVI
                     QTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKPQSFNLLNNSLEESFPKI
                     IITPSATSCQFILNDYVFSFEGLVAGTDVVIDSDLMLCYEEQSDGDILDRSNKMKTMQ
                     YPTLQVDINHFNCTGLSKIQIYRNGLR"
     gene            19087..21378
                     /locus_tag="ONT85_001867"
     CDS             19087..21378
                     /locus_tag="ONT85_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR2378836.1"
                     /translation="MIDNLITIYDKNDANNLAEHLYDTQGLGALSDWLTATVSNKLNG
                     AEIFQGTYPISGTNADLIVEGRIIQCYVDENRAKQRLRIYYAKTSVIGNTIEVKAEPI
                     FNDIRKSVLNKYDSGTEKITATQAWQNAKALAKPAIPSQFSFSSLVDTLANVKIEKAN
                     FLEFFGGKEGSILDRFHGEFLKDNNTLHHEKRLGTDHKIKAIYTKNLTGLDLEIDAQS
                     VLVGVYPFISSSSEGEDEITLPEEVIFTDYVDDYPAGYVSFVDFKDKATDVATLREAA
                     KDWLKTNIDKQKPQVSGSIELVPLRHQRGYEKFVDLEKVSMGDGVDVYHPQLKVNMSA
                     RIVEYTFNVLTNSYDKLVVGNVKTNFLENTENNVSNLINDAIDQLKNGGEISDLINDI
                     VDHQTDMITGQNGGYVLLDPKEAPSRILIMDTPDKNTARNVLQINNAGIGFSKTGING
                     TYETAWTLDGGFNASFITAGEIVGITIRGTTLISDGTDYRTSIANGKMTWYSKKVNKD
                     IMELEARDYVSADAGIVSYTMKTGGGFMIRNPQGNLVFSTWDNGNNRPFLSFGAPNFR
                     YSNASYVTSGDGSSLSINGSAGNSWEFKVAGRTMKFTSDGMLTLPGCFFGSWEDGKLA
                     RFEQSTVQVYKDFTVRGTKNSTVPTEHYGQRLLNAYETPEYYFADYGEAVTGDDGKVR
                     VDIDPMFAETVNLSRYMTHVTPTELVLCAVTHEDIDHFIIETSKPNVLVRWNLVAHRL
                     GYEDIRLKEDTAYDSTVLDQKRF"
     gene            21402..>21988
                     /locus_tag="ONT85_001868"
     CDS             21402..>21988
                     /locus_tag="ONT85_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2378837.1"
                     /translation="MASSLYNLALDFSKELNYTKAIMARQGDKGITVTVKPFLNGLQM
                     DTSGGTFTLKGTTPSNRYVDSVATSVTSEEVTFSLDGTFMSEAGYYKHCYVEYRKDNQ
                     ILTTQDIIFFSLGVSDISQGQADEYVSQLEELIQKYNETFDAFMAEIEGRVDSLNKQI
                     TDLTGQAKTLQDKLDALKEEISKLGNLQVMYSNSI"
BASE COUNT         7933 a         3246 c         4705 g         6104 t
ORIGIN      
        1 aaataagcct caaagacgaa tcgaatacaa ccgtgagtga tgccaatatc ttagcgaata
       61 tcatcctaga tttcatcttt gaacatcata ttcctttcaa agaaggatat gagattttac
      121 cagcgaatca agagtattac ttctacaaat gcattacaaa aagagtctgc tgcatctgtg
      181 gcaaaacagg agctgacatc gatcactttg acaaagcgct aggaagacga aagcgcaaag
      241 aagttgatca tgcagagtac acatttgcag cactctgcag aatccatcac acagagaagc
      301 acaaaatagg tgtgattaat ttcaaaaata aatatcaaat caaagggatc aaattaaacc
      361 agaaaacaat caaaaagtta aatattggag ggtaaaaatg acagaacatc gaagttatta
      421 tgcgattata ccagccaacg taaggtacga caaaagactt aaaccaaata ctaagttgtt
      481 atacggagag ataacggcct tgtgtaatga aagaggcttt tgttgggcag gcaatgagta
      541 ctttgcagat ttatatggtg tgaataaaga gaccatatcg cgatgggtaa gtgatttgat
      601 taagtttgga tacttgaatc gggaaatcat ttacaaagag ggtaccaatc aaataatcaa
      661 taggtaccta cgaattaatc aataccctat tgacgaaaaa cgcaataccc ctattgacga
      721 aaaagtcaaa gataataata catctattaa taatacattt aataatacaa aagaatatat
      781 aagagagtta ccgccttcga aaaaatcgaa ggctaagccc gtccgtcata aatacggaga
      841 gtataaaaat gttcttttgt cagatgagca gatggagaaa ctcaaaacag aattccctaa
      901 tgattaccaa gagcgaatcg aacgactgtc agagtattgt gaatcatctg gtaagactta
      961 taaaaactat ttggcaacta ttcgaagttg ggcaaggaaa gaaaaaagtg aacctaagaa
     1021 cgcaagcagt ggatacaagc gcacaggaag acgagagaag cttcctgaat gggcaatcga
     1081 ccaagaagcc tatctcaaga aaaaagcgct agaacgagct aatagacaat caaaagcacc
     1141 attctaagag gtggaaaaat gaagatcgat tatctagaac taattaatga aatagcgaat
     1201 tataaaaagg gcgaggaatt agacgtcctg agagacgtat atgatcaact cgaagaagct
     1261 ggaattgaag gaattaagaa tgatcgttcg agttggagta agctcagata ctatttcgca
     1321 ctttatatcg atacaacaca attaagaaat ttagcatata ccaaattact atttgttgat
     1381 tgtgttaaag gcttgcaaaa acatcttaat gaacttgagc aggtgtaatc agatggacct
     1441 aaagacattt acagcacaga ttgaactaat gcatcaagaa gctttaagac aaagtgtatc
     1501 gtacgaagac aagtggctca acacgttcca tggcggacgt gagagcgcac ttgatcaagt
     1561 actcaaatta ctgaaaggag aatgtcggga tggataagaa agcggcaatg cagcgaatta
     1621 tcgaattgac ttattcagaa gattggcaaa atgacaaaga agctgcttca gaagtgatga
     1681 ggcttggaag agcgatgtgg gcagacaaga gcaacaagcc aagaccacga aaaatcgcaa
     1741 tttggcacgg tgacaaactt ctagtgatag ggacagctga acagttagca agtctcacag
     1801 gcttgcacga gaaaatcgtg aggaaaagag caaggtgtgg ctacacagac gctaagaaga
     1861 gaacgtttag atacgtggag gaatcatcat gacaacagaa gaagtgattc aaatgcgtat
     1921 tcgaagcctt cagcgtgaga ttgacgatct ggaacgaaca aaggcagtga tggtcaatga
     1981 aacggcgaga aaggcaatcg atttgcacat agagaattta agaagggaaa tccatcgatt
     2041 ggaggaatga gcgtggataa gaaagcagca atgaaaagaa ttgctgaatt aaccaagtca
     2101 gaatcttggc aagaagacaa agaaatagtt gcagaagtcc aaaagctcgg taaatcaatg
     2161 tggactgaaa agcccaaacg gagaacgccg agaaaaattg caatctggca tgatgaccga
     2221 attttagtaa caggtactgc tgaacagtta tctgaaatta ctggattaag caaaaacatt
     2281 atctgggata gagctaggag cttatggatt gattcaaaag gacgacagtt taggtatgtg
     2341 gaggagagat aatggatctc attacacaat acagtgacat catcctcaag aaaatcatga
     2401 tgaagattca gaaagataaa aaatcaaaag aacgagctga attagttaag ttagaaatgg
     2461 ctgaaacagg agcaggagtg cgaagtagca ggcattggaa agcagcagca aacattgaat
     2521 tttattacaa cgaaattcaa aaagggttcg atcagatgcg tgagctggat cggcaaacaa
     2581 attggagcaa gaaacttcat caagatcgtt tcaaatttgt agagaagtat aaagaaatat
     2641 tagaagagta tttgaggagg acagcaaatg ataaaaaaac tcgttcaatt cagcatggat
     2701 ttatatgata tcgaatcagg agcaacacta tctgtggaat cggaccatct aatcataaat
     2761 tttggtggaa agcgccagat tattttgtgg gtagttgatg atgtactgtt tccagaaatt
     2821 gttcatgatt tcgaagaatc aaaagcggtt gagtttgaaa tagtgaaaaa agtaatggaa
     2881 ttgattgaaa aatacgagga ggacagcgaa tgataccgaa gtttagagcg tgggataaac
     2941 gaaagaacgt aatgagagat gtagccgtct tgcattttac taaaaacggc aaaacaaact
     3001 ttattgaata ttggataaat cctaccgaat tgaaatcata tcacgtgcga aacatcgacc
     3061 tcatgcaatc cacaggaatg aaagataaga atggtgtgga tatatttgaa ggggatatag
     3121 tattagtcag cgtgcgaaat ggcttcgatt acttagataa taaagtctgt attgtcaaaa
     3181 attcaataga ttattccgga ttagtttgtg ccactgttga tgaagactta gagtatcaaa
     3241 tttttaacac agagctgttt gaagaataca cgtacgaagt caccggaaat atatacgaga
     3301 atagcgagtt attggaggaa cagcgatgaa taaacaggaa gcaatcaaaa agttagaaag
     3361 tattaaagcg ataggaaatg atgcaatagc tgcttgctat aacgagagta taaattcagg
     3421 tattacgtta atgaaaaaaa tagacgaacc gcagaaacca gttattccac aactcgtggc
     3481 cggttggctt gagaaatcta cggacccttt tacaaaagct gaaaaaatag cgtatttaat
     3541 caaatctaaa gatggtgatt catattattt ctgtgattgg tttgtacgag atggcatagt
     3601 gacgcaagag caaggagaag aattactcgc ttgggcaaca agacaatcat atgaaacact
     3661 attgagccta tacaacggct acgaggttga gaaagagccg ttatgggcaa taaagaatgc
     3721 cgatggaaac tatcttacta aatgtgcttt atggggaaaa gatggagtaa attatagttt
     3781 tgaatgcaat ccatctcatc gattgctttt cactgataaa gcaacagcgg atgctgcagc
     3841 attgttggtg aatggaacag tggaagaggt ggtagaaaga tgaaactaaa agacggattt
     3901 tacgctagca gtcacggtat cggcggttta atgctagata tgccgacaaa gaaccctaaa
     3961 acacgcaaga aatcaaaatt caaagtcggt gacatggtcc gctgtgaagc agaagagttc
     4021 gtttatccgt tcagaggata cgttaaaaag atactgtcaa actcagcaat cattcgtatt
     4081 gaaaatacaa tgaagtgtga tcgttcgact gcaaaacaca agcattattt agctgtagcg
     4141 agattagttg acatggaagt aatcaagagc aaataaaaaa agccggatcg ctccgactga
     4201 ttcaataaat ccaacacatt tattatatca cataaaggag cggtttgact tgatgcaatt
     4261 gttacgagag gtagatttca aacagacaag atgtaatgcg agagatgtgc tgaagaactt
     4321 tcggcgtttg gagcggatgg caggtcgctc tttgatagat attaagtcgc cgattattac
     4381 ggatatgccg aaggcaccaa agcacggcaa taaggcagaa gacgcgatca ttcagatgat
     4441 ggatatagaa gcggagagag atgcgattct agcggctttg atggctctta gtctaattag
     4501 tcgccagata ctctactaca gcttctgtgt gccagatagc ttctcaaact acaggattag
     4561 ccgtgaagtg ggttattcag aaagaagtat acaacggatg aagtcggaag ctctaataga
     4621 gtttgcagaa gcatataaac acggaagaat aattgcttat aaataatttg gcggtttttt
     4681 ggcggaatga tggcggtttt tagctattta ccagtgatat tatggtagtg tcgaaagatt
     4741 agtgataggt ctaagacaaa ataaaatgta agggaggaaa tctccctcat cgtttaatta
     4801 agcttcgata gacagcagca aataaactaa aggatgtggg ggtcagctcc tacagttagt
     4861 tcatgtgttg ctgtctatta attgcaactc tttcggtttt attgagtatt tactcaactt
     4921 aaaaaaactg ttgtttgtag atgtaagctg ttcctatttg tacatagtaa aaaatgatat
     4981 aataaaaata ttacgaaagg ggtgagagtg catggaagac aaaatgtatt taagacaaaa
     5041 agttttcaca aaaaaagata ttgaaaatca aattaatgga tttatacaga aattagaaaa
     5101 tgcaaattct tatttgataa ataaggaact aaataaagct tatgaccagt ggaaagaagt
     5161 atatgatgag ttgaaattga ttcaaaatga aacaaaatta gtacgaacgg agaaaaagaa
     5221 tgaaaatagt ttcttttttg atggatatgc tatattgatg ttggaaactg tagcaaaaca
     5281 aaatataaaa gctcccaaaa aagaactatc tgacaatatt gataacgcat tagccgagtt
     5341 aaggtattac gttatgcaaa taaaagatgt taggatcact cgttgagtgg tctttttatt
     5401 ttgcacaaag gaggtaacaa caatgtatag accacaatac ttagaacaga agtatgaagt
     5461 aatcactgtg caaaatggta acggagagat agtacgaaag tatagaagac caataaagag
     5521 cgatacatat aaacgaaagg aaagcaatga agttattcca ttgtatggca aaagaatagc
     5581 taagcattaa ataagattgt gaaaggagac gggacatgaa cgaggaattc tatagatggc
     5641 tattacagtt gataagagaa gatcgtttgg ttaagttcta tcagtctcct aaatggcgca
     5701 ggcttagaga gaaagcgatg aaacgagatc actatgaatg ccaagagtgt agaagactag
     5761 gtaagtatca tagagtagag aacgttcatc atataaagga agtcaaggat agacctgact
     5821 tagctttaga tttagataat cttatttgtt tatgtgttga acatcataat gaagttcatg
     5881 gcagatatct tacagcattg gataaacaag agaagaagat agaaagcttt gctaacttcg
     5941 atgcaagtga aaggtggtaa gtgcatgatc atcaatgata atggcagaga gtatgataca
     6001 gaaaagattg aagagtattc atcttatact cagggattaa ttaaacgttt gatatacgtt
     6061 cgctatgtag gtattaggga tctgttatca gataactgtt gtagtaaata caaagtgaat
     6121 caagtaagag aagcgttgaa taaagataat aacgtcgaaa gaataaaaaa tgtttttgga
     6181 tatagtattg aagagattaa ttattacatt gacttcgctg aagctttcat tccgatggtg
     6241 agataacccc cccttaaaat aaatcgcaaa ttttttgggg gtgatgaaac ggagggggct
     6301 gtcaggaaaa gagatttttt cgaactttat catgaaagga gggctaaaat gtttaaaaac
     6361 gaattgtctc aaaatcgcta cagagaaaaa ttacgccgct ctttaataag ccaattggaa
     6421 agtcagaaaa caaatattga gccattctta gataatgttg atcgttatat cagtttatgg
     6481 gaaacggcga tatcactgga agaagatata tccgagaacg gcattagact ggagaatggt
     6541 aaaaagaatg aatcagtagc gttgcttgtt tctgtcaaca aacaaatggg attgatgttg
     6601 gataaacttg ccattactcc tgaattggta ggtgaagcaa atgaatcaat tcctgagtta
     6661 taagcatatt gaaaattggt tcaaagctat agaagaaggc actatcaagg tatgcaaaga
     6721 gcaattattg ctaaaaaatt atctagaaga aagagtcttt actagagaag atatttactt
     6781 cgataagcag atggtagagg attcaatcaa tataccagca caatactttc cattcgaatt
     6841 aattccgtgg gaaaaatttc tacaatgttt tatttatggt gttcgatgga aaaaagataa
     6901 aacactagtg ttcaatagat atctttcatt aatgggacgt ggtaatggta aaactggttt
     6961 tgcttcttgg aacaacttct ttctactaac cgctaaacac ggtattaaaa attatgatat
     7021 tgatatctat gccaataatg aaagccaagc aaagactagt tttgatgatg tatttaaagt
     7081 aattaaagat catcctgatt tagataaaaa agtatttaaa gctacgaagg aagttattca
     7141 aaatatcgct acaaacagca aacttcgtta taacacggca aatgctagaa caaaagatgg
     7201 gaagcgacca ggtgcaaacc gctttgatga aattcacgaa aatgaagatt attcaatgat
     7261 aaatgtggct acttctggtg gtggtaaaat tcgagattat agagaatttt atgatacaac
     7321 taatggtcat gttcgtggtg gtccgcttga tgacattata gaagaatcaa aaatgattct
     7381 ttctggagaa cttggaattg acaaggatgg agcagaattt tctagtttgt ttccatttat
     7441 ttgtcgcttg gataacgata atgaagttga tgatcccgac atgtgggaaa aagcttgtcc
     7501 aactattaat tacaatgcaa atttaaaacg gaaaatgttt caagaatact ctcaaatgca
     7561 acgtaatgct ggtttaagac ttacgttcat gaccaaacga atgaacagac ctatggaaga
     7621 tacacgattt gctgttgctt catatgatga tgttctgcat acgaaagaaa aagaatttcc
     7681 tgaaaaaatg gatgaagtga taggaacagt cgattttgct gatagacgag attttgccag
     7741 cgttgggttg ctaggaaaat acgataaaga tgtttatttt acacaacata cttttatcca
     7801 cgaatcagcc cttcgattac aaaacatcaa acgagaggtt atagatattt ctatagatca
     7861 aggaaaatca cagatcgttc atggaaaaaa tatagaagct gattatattg taggttggtt
     7921 tcttgaaatg agtaataaat attatattaa aaaaatcgct atggatatgt accgtgcaaa
     7981 aatattgaag cccgctttag aagaagcagg ttttactgtg gaaattgttc gaagcggatc
     8041 tgttacacat ggtatgttaa aagatctggt tgatgacctt tttattaatc aacgtttatt
     8101 ttttggtgac gatgcgatta tgcgttggta ttgcatgaat gtatatgaag agcatatttc
     8161 taatggaaat atacgctatg aaaaaataga acctgaaact agaaaaacgg atggcttttt
     8221 ttcattcctt catggtttga attttttaga tgatatttat gattctgctc ctgtaacagt
     8281 cacaaatagc tcagtagaaa atacaggaac tggatttact cctctagtat tctaacttga
     8341 aaggaggtga gaaagtgggg atttttcaaa aggcggtagg atacttcaca aaaaaagcaa
     8401 cggttccttt agaagaatac ttttgtaaat tgcaagttga ttttgtgtat cgaaaatttg
     8461 cgattgaaac ttgtattgat ttgattgcaa atgcgatgag taaagcggaa ttcaagtcat
     8521 atgaagatgg aaaaaataaa aagaatgatc tttactatag gctgaatgta gctcctaata
     8581 agaaaaataa tgcaacagaa tttagaaaaa aactgatcag gagattaata ttctacaatg
     8641 aagtattgat cgtttctccg tctaataatt ctagcgaaat atttattgcg gatagttggg
     8701 atgtcacaga atatgcattg aaagatgatg tgttttctca agtgcaaatt aacaacatag
     8761 tccttgatag agaatttcta gaaagtgatg ttatctatat aaaatacgca gatcaacaaa
     8821 ttaggcaact agtcgatgcg tattatcaag cgtatgggaa actcatttct agtgccatga
     8881 atgtttacaa gcgctctaac gctcgtagat acgtactgaa agggaattta ttccgaccgc
     8941 aagacaatac aacacaagat caaatcaata aaatgatgac atcacaattt aaggctttta
     9001 tggaagctga taatgcaggt gcggtatttc aattacaaaa tgagtacaca ttagaagatt
     9061 tcagcggaaa cttccaaagc aattcaagag atataaaaaa cttaatagac gacatctttg
     9121 agatgacagc agcagcgttt cacgttccga aaaacctact aaagggagac atgagtgggt
     9181 tatcggatca agtggacgct tttttaatgt tcgaaatcat accgattgct gaacttattc
     9241 aggatgcgtt taacgctagt ctctatgaag tagaagaata cttgtcaggg aattttgtac
     9301 gtgtggatac aactatgatc aagattacta gcttcaaaga tttggttgac gctattgatg
     9361 taggcattag aaatggagta tttacaatca acgaaggaag agaacgcgtt ggaaatgatc
     9421 gctctgataa ggcgatggca gatgaaatat ttataactaa aaacaatcaa caagtatcga
     9481 aaggaggtga ggcgaatgac gacaatgaaa acatttctag cagtaaagaa tgaaggcgca
     9541 gtaccgcaaa tttttattca gggatttatt ggttctagtt ggttctttga agggaatact
     9601 gacaagggaa tcaaaaatat tttggatagt ctaggtgatc aagaagaaat tgaagtagta
     9661 attaattcaa acggtggaga cgtatttcaa gggattgcta ttgggaactt acttaagtca
     9721 aataaagcaa aagttaacgt tgtgattaac ggcttagccg ctagtgctgc ttcaattatc
     9781 gcaatggctg gcgatactat aaaaatctac aacaatgcac aattgatgat tcaccgcgct
     9841 tccacatacg gagaaggaaa tgtcgatgac ttccgtacga ttgctgacca actggaatca
     9901 attgataaat cggtaaaggc ttcatataaa acacgattca atggcacaga tgaagcattg
     9961 caagaacttc ttgaaaaaga atcgtttatg gatgcagaaa cagctttgag ttatggattg
    10021 gtcgatgaaa ttatcgatgc agaaaatagc tcaggtactg aagctaaaaa agaacaaagc
    10081 gttgaagaaa ttttgaatga cgttaaagaa aaaagagcag aaaaaattgc tgcatttaca
    10141 gcagcattaa ataaaacatt tggacaagga gatgtaaaat aatgacagtt aaaaatttaa
    10201 aaggtgtaac agctgcaagc gaccaattga tgaaagcttt taaagatggt aacgaagaat
    10261 cttttagcgc agctatggta agcttatcta aggaaattca ggataaaatt ttagaagaag
    10321 caacagcaaa aaatcaagat caattagtat taatgaaccg tggtcagcgt gtattaacta
    10381 cacaagaaac aaaattctat aacgaagtag tgaaaaacga aggttttgca ggggtcgaag
    10441 aattagtgcc agctactgta tttgaacgtg tatttgaaga tttagaacaa tctcatccac
    10501 tattgcaaaa aattactttt gttaacacaa ctggtgtaac agaatggatt gtgtcacgtg
    10561 gagtcaatcc agcatggtgg ggtaaacttt gcgaagctgt taaaaaagtt ttagataatg
    10621 gctttgatgt aattaacatg aagcagttca agctatcagg ttatattcct gtatgtaagg
    10681 caatgcttga tttaggtcca gtatggttag atcgttatgt ccgtactgtt ttagtagaat
    10741 cattgagaat tgcattagaa caagcaattg ttgatggtac tggtaaagat atgccagtcg
    10801 gaatgatgcg tgacatgagc aaacaaacta gcggagaata tgctgaaaaa aaagcagaac
    10861 ctattacagc tttagatgct gtaactatgg gccgtttgat ggcgcgacta tcaaaattca
    10921 atatcgaagg tgtgaatgat ccgatttatc gtaatgtgaa tccttctgat gtggtcctaa
    10981 ttgtgaatcc aactgattac tggtctaaag tattcccagc taagactgta ctaactgcta
    11041 atggagaata cgtacaagta ttgccagtac cagtttcaga tttgcagtca acggctgtgc
    11101 cagaaggaaa agcagttatt ggggtagcct cagattactt catgggtgta ggatctacac
    11161 taaaaattga agcttcagat gaataccatt ttgttgaaga cgaacgcatt tatctagcta
    11221 aacaatatgc aaacggtcaa cctaaacgta acgatagttt cattgtatta gatattagcg
    11281 ctttgggaac tactactaca actacaaaac caacaaccac aacaactaca acacaagcgt
    11341 aggtgatcag aatgaagtat attctttgtc agccggcaat caatcggttt aaatgggagc
    11401 ttgaagtttg tttaactaat ctgaagaaac taggaatcaa agatatcgta ttgcttttca
    11461 gcagacacga tgatcagatt cctatttttt ttgagaagga atatggtgtt gaagttcatg
    11521 tgtacgatga tctgcgggac gacaaagagt atattccttc gattaaacca tatttatggt
    11581 ggaaatattt agaagaagat cattcgcgtg aggacgaccg atatttctat atcgattcgg
    11641 atgtcatttt caataaaaga attaatttgc gcaaattgcc ttctaaagat gatgtttggt
    11701 attgtagcga ctgctgtagt tatctaagtc ttgattatat tagaagctgt gaaaacggag
    11761 aaaatattct aaaagatatg gcaaacattg taaatgttac agtagaatct ttggaaacta
    11821 taaacactaa ttcaggaggc gcacagtggg ttattaaccg tcctaaagcg aattattgga
    11881 aaaaggttta tctggattct aatcggctat atcgctacct tagagggcaa aaaacaaata
    11941 tacaaatctg gacagccgag atgtgggcac ggctttggaa catgatgtat ttcaatattg
    12001 gtcctaaagt tcacgaggaa ttagactttt gttttgctac tgatccaata gaaaaagtta
    12061 aagaagtaaa aatcttacac aatgctggag taacaacaaa cgatgaagat ttatttttca
    12121 aagggagata cgtgacttct acgccttttg atgaagattt atcatttgta aacaagaaaa
    12181 aatgctctta cgcatatgtt aaagcaatta aggcggtggt tagatgacgc ctgaacaagt
    12241 gactgaagaa ttgctaatag ctgtgaagga taatatttac gttacctgga acgaagaaga
    12301 tgagtcaatt aaaaagatga tagctaaaaa tgctgtttat cttcaaagta aagtgagtac
    12361 aacactttct ttttctcctg aaagcttaga atacggattg ctaatcgaaa gatgtagata
    12421 cgactggaat cgtgctttag atgagtttga acaaaatttc gctagtgagt tattaggttt
    12481 cattcaacat tatgcgctac aagaatatat tgcaggtgat gtgaatggcg aataatcgta
    12541 gactcgaaga aacattcaac gatggttggt taaagatttt gacgcaaact accaaaagaa
    12601 atgaactagg aaaaaagatt ggtgtagaag atacagaaat cacttcttta aaatttagaa
    12661 atctttccat gagagatagt gatataacag ctatggatgc gatgggatcg aaattaacta
    12721 agaaagtaaa gactccattt catccaatcg ccaagaaatt taataaagat caatatttta
    12781 tcgtaatcga tagtatgcgt tacaacgtta tctatgccga ttacgataat ttttatatct
    12841 atttttatct tgaaagtgtg ggtgaatatg gtgattgata attctaaaga aaaagaacgt
    12901 ttaaataagc aaatttctgc tatcaaaact tccttagaag agcattttgg cctcaaactc
    12961 tttcaagact ccgtaggcga ggatgagcta cctgatgatt ttaattactt cattctcgaa
    13021 acaggagaaa tagaaatgat cactgagcca aaatatagcg tgggtcaaaa tctatatcta
    13081 actttctatt cagaaaatag agaagattta acaggagact cactagatat tatttcattg
    13141 attcaaaatc gttcgattcg ttttcagaga atggatccca atcatttaaa actagagaac
    13201 caagatcgct atatcgatca attggtattt acgtttagac gattattgaa gagtgattgt
    13261 catggctaaa aatagttggg agctaaaaat aaatggacat gatgaacttc ttgtgcggat
    13321 ggaacgctat tcaagcgaga gcgaacgact gattaacgaa gcattgaaat caaaaggttc
    13381 ggctattgca gtggatagga ttacagaaaa aattcctgtt tctgaagcag atttaagaag
    13441 agggcaccaa cacgcaaaaa atagtcgtcc acttaagact caatacatta atttgggttt
    13501 catcattaga cctacaagaa aatttgagta tttaaaatat cctgatttgg ggataggtac
    13561 ttctaaaaga aatcagccgg acgaatttat gagaagagga ttaggtcttg cacttgatcc
    13621 aattacagaa cttctgattc gtcaattcga taaattaaat aaataggggg aacaacaatg
    13681 gctaaaacaa aaactgtagt aacaacgttc gataacgtaa gtatcaaacg aattgctttt
    13741 aattttaaga acgcaggaaa tgcaatcgca acgaattgta atggacaatt agatggcgaa
    13801 acagaaatgc aaacggtggt taaaaaatgt ggagcgacag aagtaaaatc aaaatctaaa
    13861 ccaatcaata tgacggtaac aattactgca catgtaccga tggaagttta tcgacgtttc
    13921 aatgggttga aacaagatgg acgtattaaa ccaggcattt actcttacgg tcctgattcc
    13981 gtaggcgaag atttctcact tgctgcagag atcgtggatg acttcgaaga aaatagaaag
    14041 ttagttggta tgttagcatg cacttcgaat acaggattaa cattctctat tgaaaatggt
    14101 gcggatgaag tagctgcgtt agaactagaa acaaaagtta tgcaagatga atttggtaaa
    14161 ttttatcatg aagcaattgt tgcagaactt gaagaagact taacagatca atggatgacg
    14221 aatctatctg ctgatgtgat taaaaaaact tcagttgtga caactacggc cactcaatca
    14281 cagtaaaaaa aacggaggta gcgaaatgaa cgaagattac tcaaaaattg aactaaacga
    14341 tggaacaatt ttgaatttag aacctaaact gaatatcaag aaattattga tgatcaatag
    14401 agattttaac acagacgagt ttgcaaaaat gactgtggga aaaggatcca tggatatttc
    14461 tgttattcaa ggtgcaaagg ctgtgtatat tgcttaccgc caagcgaaca tgactgatta
    14521 tatttcattc gatgaattta tcgataaatg ggattttgat atggctactg ccagctatat
    14581 ttatcaattg atgatgttca aacaagcacg cgatgcttat caaaaagaat ttgaaaaagc
    14641 aaataaggaa aaaaagcttc aaaagtaaaa atgccaaagc tcttagttga aacgtgggtc
    14701 gatgtctatt cgatgttgac cgacgttttt tctatgcctt cagatttggt tttaagcgat
    14761 atctgtttag atgacatttt gcaaatggct tacaacaaga gtgcttatga aggatggaaa
    14821 aactatgcaa taaaccaatc tcagaaaaac taaagaaagg aggtaaaaaa tggctaaaaa
    14881 gagaacagaa gcagaagtaa ctttcatagc taacgatgac ggattgaaat ctacgttaaa
    14941 agaaatcagt gctgaattaa ctaaaaatag agcagaatta aaactagaac aagctcaatt
    15001 acaacagact ggttctgaat cagacaagtt aggaagtaaa ttatcttctt tagaaaagca
    15061 gtatgaatta caaagccaaa aagttgaagt aactagccaa cgtttagcca atgccaaaaa
    15121 atattatgga gaaaattcca ccgaagttca gaaacttgag agagaactga ttaaccaaca
    15181 aacagcgcaa caacgtttgt caaacgaaat tgataaaacg agtaatacac tagctcaagc
    15241 aaaaggcgaa atacagacgt acgagtctac aatgcaacag ttggatagtg aacaaaaaaa
    15301 tgttcaagct agtgcttctc tgattgaatc cgaatacaaa aaatggcaag taactgctgg
    15361 tcaatcagct tctgaatccg agaaattagc gaaagcccaa gaatatgttt ctcaacaatc
    15421 tgaaaatgca gagaaaacga tagatatcct gagacgacag ttagaagcta cacagtctga
    15481 gtttggcgct acatccacag aagcaatgca gatggaggcg aagcttaatg atgctgaacg
    15541 tgaatttgaa gagttaggac aagctgctaa aaatgtagat acaactaact tggacgatat
    15601 cggaagcaaa atagatatga ataatttaat ggaagcttct gacgttttaa gcgacattgg
    15661 cgataagctt acagaattag ggaaacaagc agtggactct gctaacagtg taggtagttc
    15721 ccagagtaaa atacaagcta attttggttt gactaaacaa gaggctgaag aattaacgaa
    15781 tgtagccaga gacatttatt ataaaggttt tggagaatcg ttagatcagt ccacagatgc
    15841 attgattttg gtaaagcgta atttaggcga tttaaataat caagatttac aaaatattac
    15901 ggaacaagct atggtcctag aaaacaccat gggcgctgat atggatgaaa cgttacgtgg
    15961 tgtaaatggc ttaatggtca acttcggatt gagcgctcaa gatgcaatgg atttaatggt
    16021 ttcgggtact caaaacggtt tagataaaac gcacgaatta ggcgacaata tggcagaata
    16081 tagccaatta tggagtcaaa tgggatattc agctgatgaa acgttcggaa tgcttcaaaa
    16141 tggtttagat gcgggtgctt ataaccttga taaagtcaat gacttagtta aggaaatggg
    16201 aatatcgtta acagatggtc gatttgagca aaacatggat atgtttagtg aaagtactag
    16261 aaaagctttt gaagagtgga aaaatggtgg aggaacacaa aaagacgtta ttaattccat
    16321 gattcaagat tttagcaata tggatggtca atacgaccaa ttaaataaag cttctacaat
    16381 ttggtctgca cttggcgaag ataacgcgat gaaagttgtc caatctttga ctgatgttaa
    16441 ccatacattt gatgatgtta gtggatctgc acaaaaaatg aatgaagatt ctactactcc
    16501 gttacaagaa ttaaacggaa aaatagctga attaaaggat tcattagctc ctataggcaa
    16561 cacaatcata gatgcactcg aaccagtaat tgattttcta ggaaagatgg ctgatgcgtt
    16621 taataatctt ccacaaccag tacaggatta tgccgtagcg attggcggat tgactgctgc
    16681 atttacttta ttaatgccaa taatagttgg cttcatggct ctaggtggtc ctactacatt
    16741 aataatagga gcagttatta ctgctattgc tggagttata gcaattataa aaaactgggg
    16801 cgcaattact gactggttta agggaatatg gagtaaattc actgattggt tgggtggtac
    16861 ttgggaaagc ataaaagaag gtgcctcatc agtttgggat ggagtcaaag aaacctggtc
    16921 tggatttgta gattgggttc aagaaatttg gcaaggagtt tctgattggt ttggagagtt
    16981 atggagcgga ttagttgaag gagcttccaa catctggcaa ggagtccaag agacttggca
    17041 agcattcgtt gattgggttt caaatatttg gaacggagtc aaagaagtat ggtcgattat
    17101 ttgggcagac attgtaggaa ttgttcaaat accatggaca ttaataacgt cattgattca
    17161 agctggtatt aatattatcg tgggtatctt tgatgtagct ggacagttat taggcgcagc
    17221 ttggcaagct gtttggacac ctatttctga tttccttaaa aatacttggg atactatgac
    17281 acaatggata agcatcgctt ggaatggaat tgtaactaca ttccatacta tatttgatcc
    17341 agtagtggca tggtggaatg gtatatggac atctattagt actacggctt caaatatttg
    17401 gaactcaatt agtgcaacag cttctagtat ttggaatagt atcaagaata caatcactag
    17461 cttggtacaa gcagctgcta cagtaattca aaatatttgg tcaactgtat ctagttggtt
    17521 aggtggaatt tggaattcaa tcagctctac agcatcaaat atctggagta gcgtgactag
    17581 tagtataagc aatgctataa acgcagctaa aagtgccatt caaagtgttt ggaatagtat
    17641 atcttcgtgg atcagcggaa tttggaacgg tatcaaaaac actgctttga atctttggaa
    17701 tggaattaca agcactatta gctctaaagt aaacgatgga aaaaatgcaa tttcaagcgg
    17761 ttggtccaat ctaacaggta ttgtttccga catattcaat aatgttaaaa gcacgatagc
    17821 taatatatgg gaaggtatca aaaagactgt tagcgctccg attgattgga ttagagataa
    17881 aatcagtagt gtctttgata atttgaatat ttctatacca catattccgt taccacattt
    17941 taaattgagc ggagaattca atccattgaa ggggaaaatc ccaacattgg gtgttgattg
    18001 gtatgcgaaa ggtagtgtgt ttaattctcc aaatattatc ggtgtaggtg aatcaggacc
    18061 tgaagcagtt ttacctttga aaagatctgt gctgcaagaa attggtgatc gtatcttgag
    18121 tagcacctca gtttcatcta ggacacaagc ggttcaacct gtgaacaatt acgaattcaa
    18181 tttcacaatt gatggtaacg cagatgaagt cactatgaag caaacaactc aacaaatcat
    18241 tgatagcatt acaaaagttc aaaatgataa tgcttcggca tggcgttaaa caggagagta
    18301 tttctcctgt ttttttagta ttaaaaagga tgtgaaaaaa tgactgattg tatacattct
    18361 ataatcgatg gatttcctga ttatttgcat aaattggctt tagctgaaag accaaccata
    18421 ccttctccaa aaagacagag agttgaaact tctgttttag gaaggttagg tggcttagtg
    18481 caagattact cgtttgaaga catgtcgttt acattgcact ataactattt agaggatgtg
    18541 gaagaccatc aagcgttcaa gcaatcgttt tatatcatgc gtcattggtt aaactatgca
    18601 aagaaattag aattctctga cgatcccaac gtctattacg ttatccagac tatcgatatt
    18661 ggggatgcag aaaacgatat tgttgaatgg ggagagttcg atgtaaatat tactgcgaaa
    18721 ccattcgcaa gagttcaaga agatgtacct ataaccgtag ataaaccaca gtcatttaac
    18781 ttgctgaata atagtttaga agaaagtttt ccaaagatta tcatcactcc ttcagctact
    18841 tcatgccagt tcatcttaaa tgattatgtg tttagtttcg aaggcttagt agcaggaact
    18901 gatgtagtca ttgatagtga tttgatgctt tgctacgaag agcaatcgga cggagatatt
    18961 ttagatcggt ccaacaaaat gaagaccatg caatatccga cattgcaagt ggatatcaat
    19021 cattttaatt gcactggttt gagcaaaata caaatttatc gtaacgggtt aaggtaggtg
    19081 aaatagatga tcgataattt aataactatt tacgataaaa atgacgcgaa taatttagct
    19141 gaacatttat atgatacgca aggtttaggc gctttgtcag actggttaac agctactgtt
    19201 agcaataaac taaacggagc cgagatattt cagggtactt atccaataag cggaactaat
    19261 gcagatttga ttgtagaagg acgtattatt cagtgttatg tagatgaaaa tcgagcaaaa
    19321 cagcgtctac ggatttatta tgcaaaaact tctgtaatag gcaatacgat agaagtaaaa
    19381 gctgaaccta ttttcaatga tataagaaaa tcggtgttga ataaatatga cagcggaaca
    19441 gaaaaaatca ctgctactca ggcatggcaa aacgcaaaag ctttagcgaa accagctatc
    19501 ccttcgcagt tttctttctc gtcattagta gatacgcttg ctaatgtgaa gatagaaaaa
    19561 gcgaattttt tagaattctt tggtggaaaa gagggatcta ttctagatcg atttcatggg
    19621 gaatttctta aagataataa cacattacat catgaaaaaa ggctaggtac ggatcataaa
    19681 atcaaagcga tttatactaa aaatttaact ggtcttgact tagagataga tgctcaaagc
    19741 gttttagttg gagtttatcc attcattagc agttcttcag aaggagaaga cgagatcact
    19801 ctaccagaag aagtcatttt cacggattac gtggatgatt atcctgctgg atatgtttct
    19861 tttgttgatt ttaaagacaa agcgactgat gtagccacat taagggaagc tgctaaagac
    19921 tggttgaaaa caaacataga taaacaaaaa ccacaagtga gtggttcgat tgaattagta
    19981 ccattgaggc atcaaagagg ctatgaaaaa tttgttgatc tagaaaaagt ttcgatgggt
    20041 gacggagtag atgtgtatca tccacagtta aaagtgaata tgtcagcgag aattgtggaa
    20101 tatacgttta atgttctaac caattcatac gataaattag ttgtaggaaa cgtcaaaaca
    20161 aacttcttag aaaacacaga gaataatgta agcaatttga ttaatgatgc cattgatcaa
    20221 ttgaaaaatg gtggcgaaat cagtgattta atcaatgata ttgtagatca tcaaactgat
    20281 atgattactg gtcaaaatgg tggttatgtt ttattagatc ctaaagaagc gcctagtcgt
    20341 attttgatta tggacacacc agataagaat accgcaagga atgttttaca aatcaacaat
    20401 gctggtattg gtttttctaa aactggcatt aatggaacat atgaaaccgc atggacatta
    20461 gatggcggat tcaatgcctc gtttattacg gctggtgaaa tagtaggaat tactattaga
    20521 ggtactacat taattagtga tggcactgat tatagaacaa gtattgctaa tggcaaaatg
    20581 acttggtact caaaaaaagt taacaaagat attatggagc tagaagcacg tgattatgta
    20641 agtgctgatg ccggtattgt atcatacacc atgaaaactg gtggtggttt catgattaga
    20701 aatccacagg gtaacttggt ttttagtacg tgggataatg gtaataacag accgtttcta
    20761 tcttttggtg cgcccaattt caggtatagc aatgctagtt atgtaacttc tggcgacggt
    20821 agttctttaa gcattaatgg tagtgcgggt aactcatggg aatttaaggt agctggtagg
    20881 actatgaaat ttactagtga tggtatgcta acgttgccag gttgtttttt tggttcatgg
    20941 gaagatggga aacttgctag gtttgaacaa tcaacggtac aagtatataa agattttact
    21001 gttagaggta ctaaaaactc aaccgtacca acagaacatt atggacaacg actattaaac
    21061 gcttatgaaa ctccagaata ttatttcgct gattatgggg aagccgttac aggtgatgat
    21121 ggtaaagttc gtgttgatat tgaccccatg tttgctgaga cagtaaatct aagtcgatat
    21181 atgacacatg tgacacctac agaactagtt ttgtgtgctg ttactcatga agatattgac
    21241 catttcatca ttgaaactag taagccaaac gtattagtta gatggaattt agtggcacac
    21301 cgtctagggt atgaagatat tagattaaaa gaggatacag catatgatag cacagtgctt
    21361 gaccaaaaac gtttttaaaa cgaagacaag gaggtatata aatggctagc agtttatata
    21421 atttggctct agatttcagc aaagaattaa actacaccaa agctattatg gctcgtcaag
    21481 gtgataaagg gattacagtg actgttaaac catttttaaa tggcttgcag atggatacga
    21541 gtggcggaac atttacttta aaaggaacaa cgccatctaa ccgttacgta gatagtgtgg
    21601 caactagtgt aactagtgaa gaagtcacgt tttctcttga tggcacattt atgagtgaag
    21661 caggatatta taaacactgc tacgtagaat atagaaaaga caatcaaatt ttaacaacgc
    21721 aagatatcat ttttttctca ctaggagtgt ctgacatttc gcaaggccaa gccgatgaat
    21781 acgtttcgca attggaagag ttgattcaaa agtataacga aacttttgat gcttttatgg
    21841 ctgaaatcga aggtagagtg gacagcttaa ataaacagat tactgattta actggtcaag
    21901 ctaaaacact acaagacaag ttagacgctc tgaaagaaga aatttctaaa ttaggtaact
    21961 tgcaggtgat gtacagtaac agcatcga
//
LOCUS       DAJSBL010000040        21910 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.040, whole genome shotgun sequence.
ACCESSION   DAJSBL010000040 DAJSBL010000000
VERSION     DAJSBL010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21910
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.040"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(459..1088)
                     /gene="udk"
                     /locus_tag="ONT85_001869"
     CDS             complement(459..1088)
                     /gene="udk"
                     /locus_tag="ONT85_001869"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HCR2378838.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1172..1867)
                     /locus_tag="ONT85_001870"
     CDS             complement(1172..1867)
                     /locus_tag="ONT85_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HCR2378839.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1880..2596)
                     /locus_tag="ONT85_001871"
     CDS             complement(1880..2596)
                     /locus_tag="ONT85_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HCR2378840.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2926..3534
                     /locus_tag="ONT85_001872"
     CDS             2926..3534
                     /locus_tag="ONT85_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HCR2378841.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEEESTGEGGEPEVIGSTEE"
     gene            3859..4182
                     /locus_tag="ONT85_001873"
     CDS             3859..4182
                     /locus_tag="ONT85_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HCR2378842.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4364..5734
                     /locus_tag="ONT85_001874"
     CDS             4364..5734
                     /locus_tag="ONT85_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HCR2378843.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5864..7165)
                     /locus_tag="ONT85_001875"
     CDS             complement(5864..7165)
                     /locus_tag="ONT85_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HCR2378844.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYYYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKTE"
     gene            complement(7519..9477)
                     /locus_tag="ONT85_001876"
     CDS             complement(7519..9477)
                     /locus_tag="ONT85_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HCR2378845.1"
                     /translation="MTGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGLSGINPDFVIGS
                     VSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLIIPAMIGGAALLA
                     DGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFMVQRFGTDIVGK
                     AFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKLGLFILGNIFLA
                     TTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQNPEAQQIKNLNP
                     FFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPRLKIMYPGNSIG
                     QMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALLYFYLIQNGHSR
                     WLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWEQGNIIRESSTE
                     DVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSILDKRPKRARVY
                     WFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQVIHDLMKQGRL
                     PKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAIKKIATSPETWF
                     GLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(9654..9920)
                     /locus_tag="ONT85_001877"
     CDS             complement(9654..9920)
                     /locus_tag="ONT85_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HCR2378846.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(10430..12556)
                     /gene="pnp"
                     /locus_tag="ONT85_001878"
     CDS             complement(10430..12556)
                     /gene="pnp"
                     /locus_tag="ONT85_001878"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HCR2378847.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(12744..13013)
                     /gene="rpsO"
                     /locus_tag="ONT85_001879"
     CDS             complement(12744..13013)
                     /gene="rpsO"
                     /locus_tag="ONT85_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HCR2378848.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            13264..13827
                     /locus_tag="ONT85_001880"
     CDS             13264..13827
                     /locus_tag="ONT85_001880"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HCR2378849.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(14080..14601)
                     /locus_tag="ONT85_001881"
     CDS             complement(14080..14601)
                     /locus_tag="ONT85_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HCR2378850.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(14604..15446)
                     /locus_tag="ONT85_001882"
     CDS             complement(14604..15446)
                     /locus_tag="ONT85_001882"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HCR2378851.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(15439..16164)
                     /locus_tag="ONT85_001883"
     CDS             complement(15439..16164)
                     /locus_tag="ONT85_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HCR2378852.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEIAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            16340..16636
                     /locus_tag="ONT85_001884"
     CDS             16340..16636
                     /locus_tag="ONT85_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2378853.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     gene            17204..18796
                     /locus_tag="ONT85_001885"
     CDS             17204..18796
                     /locus_tag="ONT85_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327538.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HCR2378854.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            18809..19549
                     /locus_tag="ONT85_001886"
     CDS             18809..19549
                     /locus_tag="ONT85_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HCR2378855.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            19717..21066
                     /locus_tag="ONT85_001887"
     CDS             19717..21066
                     /locus_tag="ONT85_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR2378856.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(21194..21784)
                     /locus_tag="ONT85_001888"
     CDS             complement(21194..21784)
                     /locus_tag="ONT85_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HCR2378857.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVIVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
BASE COUNT         6293 a         4462 c         4158 g         6997 t
ORIGIN      
        1 gggggtgcga caaaagagct tcttcaaaaa aataagtcga aatctcacac aaattcgaga
       61 aataattttc gtaagtttct tcttatttat tgcatctgac cacttctgtt acaacctctc
      121 gttaaacggt gttcaaaagc ttgcatctac gataataagc ccaacccaac gaaaaacgtg
      181 aggagctgtt ttatttggat gctcttatcg cttgatgcaa agacagcttt tttacaacct
      241 ctacttcacg gtgctcaatc agctgacctt cggtattagc tcaaaaaacg gtaaaatata
      301 aacagctatt ttccgtttct tctttttaat acttaggtca agatgctgtt ttcacaacct
      361 cttgttataa ataaaaaaac agttggctgg tgactgttca aaaagcagaa tccttgcttt
      421 tcaaacggtc gcagtccgtt caactgtttc gctgcgcact atttatggtt taagaaacta
      481 gctacttttg ttgtaatcaa atcaatagca acgtggtttt ctccgccttc tggaacaatg
      541 atatccgcat agcgcttcgt tggttcgata aattgatgat acatcggttt aacaactgtc
      601 aaatattgtt cgatcacgga gtccaatgtg cgtccccgtt cttccatatc ccgtttgata
      661 cgacgaatga tacgaatgtc atcatctgta tctacgtata ctttgatatc catcaagtca
      721 cgcagacgtt cgtcttctaa aatcaggatt ccttctagaa tgatcacttc ttttggttcc
      781 tgaatgatcg ttgcctgact tcttgtatgc gctacatagt catacacggg tttttcaatg
      841 gctttatagt tcagtaagtc gcctacatgc tgaatcagca gatcattatc aaaggcaaat
      901 gggtgatcgt aatttgtgtt caatcgttct tcaaaactca aatggctctg atccttgtaa
      961 taagaatctt gttcaagcat catgatggaa tggtccggga agttgttgaa aatcgcacgg
     1021 ctgacacttg tctttccgct tccggatcca cccgtcacac caatgataat tggcttgctt
     1081 tttgtcattg actaaaacct ctttcgtatc cgttttttca ttctaatcta ttatagcgaa
     1141 aaaacacgaa aatgctatat ttttctgaaa attaatcctt ttcaatttcc aataatgaat
     1201 aaagttcctc taatgaatcg attcgataag taggctggat ttcttcgatt tctggtaatt
     1261 tatttctatt gaaccaaatc gtatcgatcc ctgccatatt tcctcctttg atatcagaag
     1321 tcaaagaatc accaatgatc acagtttgct ttttatcaaa attaggaatg tgggaaaaga
     1381 catgatcaaa aaattctttc atcggctttt gcgctccgac agcttctgag acaaagatat
     1441 ccttgaagta atcaagcatt cctgcatctg ttaatcgccg atgttgcgtg tgcgacacac
     1501 cgttcgtcac aatataaaga tcagcatgcg gatggacctt tttcaggatt tctagacttc
     1561 ctggaacaag atcatgacct tggttcaagt agtggcggta gcgtttttcc atttgatcac
     1621 tatctactgt tttcccaaat tgctggaaaa gtaacccaaa acgactgcct attatttctt
     1681 ctcgtgatag tctgcctttt tcaaactcct gccataaatg atgattcaaa gccttatagc
     1741 tggtttcgat ttctggtgtc aatgtgattc cttgttctgc aaacaaagca cttaaagcat
     1801 tggcttcagt taattgaaaa tctaacaatg tatcatctac atcaaacaat aaagttttat
     1861 atttcatctt tttgacctcc tatttaaaat aaatacttaa ctttttcatc tctgctaatg
     1921 tcaacgatcg atactcgcct actcctagct gttcatctaa ttccaatggt cccatgctta
     1981 gccgtttcaa tgcggtcact ttctttcctt gggctaagaa catctttttg acttgatgga
     2041 attttccttc ttggatcgtc aataaaaccg tactttggtg cttgtctgct ttgagtattt
     2101 ttaaatcagc cggcttacat tgatacccat cagcgaatac gattccattt tcaaatgctg
     2161 ttacgtctgc cggtgtcacc ctttcgttca caactacttg ataagtcttt gaaacctttt
     2221 tttctggaag tagcatttca tatcccaatt gtccattcga agtgatcagc aatagtcctg
     2281 ttgtatctct atctagtctt cccactggat ataatggttg gaaacgatcg gcacttgcaa
     2341 tcagatcgat tactgtcgtc ttttctggat ctgatacagc tgtaacagct ttttcaggtt
     2401 tattgagaag ataataggac tcatttgttt ctaagtgttc acctgctact tcgatttgat
     2461 gcagtaaact atctacattt cgattttgtc gaaattcgac tgtgccatct atcatcactt
     2521 gcttcaaagc aaacagccgc ttcatttcct tgcgtgtcgt ttctaatttt tcttctatta
     2581 atttatctaa acgcatgctg tttcctttct tattactact aattaattat accttgaata
     2641 ttcttgcttg caaatacatc atcatagtta ttccttgact taattaacag ttcacgttac
     2701 ggttaaatag taaagtgaat tggatttctc atacacagcg taatgacctg gtggagaaaa
     2761 gttgaatcct gactttctaa ggggagatgc gcgcatctct ccttttgcgc ttttttaagc
     2821 gccctgcctt cgatgatgtg cacgagatta gcctttagaa ttcacttaaa taaaatctgt
     2881 ccgttaccaa agcagacagt cacattttaa ggaggaattt tggttatgtc agtatcatta
     2941 gaagtaagta aaagagaagt acgtccccgt tcattaagaa acaaacttcg tcacgaaggg
     3001 aaagttccag ctgttgttta tgggtataaa gtagaaagta cccctatctc attcgatggt
     3061 ttgacactaa gcaaattgtt gcgtgaacat ggagcgaaca cagtcatcac gatggacatc
     3121 gatggacaaa aagtcaatac attggttcac aagatccaaa cgaacacttt taccaatcat
     3181 ttcgaacatg cagaatttct atctgtaaac atgtcggaag aaacagaagt cgaaacagaa
     3241 gtcgttcttg ttggcgaatc agtgggtgct aaatctggtg gagtgttagc tcaaaacttg
     3301 tatacagtag tcgtatctgc tacaccagac aaattgcctg aacgaattga agttgacgtg
     3361 acaaacttgg aattaggtga ttctattaca gtagccgatc ttccaaaaaa cgacgattac
     3421 aaattcgtga ccgacagtga agagcaaatc gctgctgtag tcgctcaaag tgaagaagaa
     3481 gaaagcacag gtgaaggtgg cgaaccagaa gtgatcggtt caacagaaga ataatactga
     3541 aacaatcagg tggtgttaga agcggcgggt cagcagcttt taacacgcct tttttatttc
     3601 taatcgttta ttttcatatg gggcttccac ttttgtaaat ggctttacaa acgaaaaatc
     3661 ctaatttacc aagcttttcc tcgcatactt ttatcgtacc tttataaaaa tttatacttt
     3721 gaaaaaaagt attcaattat tgttgcattt ttcctgattc tttattagaa tgaaccctcg
     3781 ttgaagtaag tatttctagt ttatcctagg attaattata aaaataaaac attcgttgat
     3841 tcgaaggagg ttaaacagat gtttgaaagt tttgatagca gccgcaaccg ttacgcttca
     3901 gttggtgtcg tatcaagttt gccagatgaa ttgattgaca gtatatggtt tattattgac
     3961 ctagatctta agggagtcat tccattagaa aatatattaa cctttgattt gatcgaaaat
     4021 cgtggaaaag tgacgatgca tttttctcaa gcaggaagca atgtggaaat ggatattgat
     4081 ttgccgtttg cctattcgaa ccaatttcct gctcaagtat acgcctatga tgacggtacg
     4141 agagaaacga tccttttacc aagtgagatt agacaatatt aaggtgatag ataaacgtgg
     4201 atgtccctta ttttacagac caggccaagt tgttgattct ttgactcaat tcttggcttc
     4261 tttttatgga gtttcataaa ttatcattga tttctaattg gtattctcag gataaacgct
     4321 atatactact aactaaattt taaagatata aggagtttaa aagatgcgcg atttaacaaa
     4381 aggaagcccc gctaaactga ttttgatgtt cactgttcct ttactgatcg gaaatgtttt
     4441 tcagcagttc tacaatatga tcgatatgat catcgtgggt cagacgctag gtaaaaatgc
     4501 gctggcagcg gttggtgcaa ctggaagcct gacatttttg atcattggct ttgcccaagg
     4561 tttaacagct ggtttagcca ttattaccgc acaacgttac ggtgcaaaag attatcgggg
     4621 tctgaagaaa agttttgctg ctagtgttgt cattagtctt gtcgtcactg tgattttaac
     4681 tgtcctaagt ctcctgttta ttcgtccgat gcttcaattg atgcaaactc ctcctgaaat
     4741 acttgatcaa gcacaaacat ttatttcaat tattttatta gggatctttg catcagtcag
     4801 tttcaatttg ctttctaatg tcattcgcgc acttggtgac agccgcacac cactattctt
     4861 tctgatcatt gcggtcatca tcaacgtcgt acttgatttg atctttatta tctattttgg
     4921 catgggtgtg gaaggtgcag ctattgctac agtaatcgca caagtttctt ccagcgtact
     4981 gtgtctagtg tatatcaaga aaaaaatgcc tttgttacag ttacgcaaaa aagatttcaa
     5041 atttgaaaaa gaagagtttg ctgtacacct aaatgctgct ttgccaatgg cgtttcaatc
     5101 ttcgatcatc gctgttggtg ctatcgtgtt acaagcagca ctaaatagtc tagggacaga
     5161 tgtcgtagct gctcaagccg ctgctggtcg tatcgaccaa tttgcaaatc agccgatgat
     5221 gtcatttggt attgcaatgg caactttcac tgcacaaaac tatggtgcga aagaatatgg
     5281 ccgaatcttg caaggggtac gtcaaacttt actgatgagt gtcggattta gtctcttagc
     5341 aggtgctatc gtcattttct tcggtcattt ctttgttcaa ttgtttgtta gcccatcaga
     5401 aacacgtgta tttgaattag cacaaaccta cttcaatatc aatgggtctc tttactggat
     5461 cttagcagtc ctgttcattt tacgctacac acttcaaggg cttggacaaa gcaagatccc
     5521 tacgatcgcc ggtatgatgg aattgcttat gcgttctttt gcagctatcg ttcttacagg
     5581 aatgtgggga tacccaggag cagcagcagc ttctccttta gcttggatcg gttctgttgc
     5641 cgtgttgctt tactcttata tgcgttcgat gaaacaattg aaacacatgg gtgaagagga
     5701 acatttcaca ttagaacatg cagaagcaag atagccttga ttaaaagaaa tcgttgcggc
     5761 taaaaatgaa aacttttatt gaaaatatct tcttcgttta ccgaaaacag gttttttaga
     5821 taacaaaaag gctgtgacaa aaaatgtcac agcctttttt cttttattct gtttttcttt
     5881 ccgtggactc tactttgaaa taatcgatat gtgcatgctg gtattctaat accgtgaaac
     5941 ggaatgatgc aatctcaatg attgttcctg cttttatttc tttatatcgg ctagaagcaa
     6001 agcccgcaag tgtagtaaaa ttcgtttctt caaactctgg aatctctaca tgaaaataac
     6061 gctggaaatc atctaatgtt agtttgccgg agatgatata agagccatct gactgttccg
     6121 ctatcaatgc ttcgtccaaa tctgtctcat ctcggatttc cccgacgatt tcttctaaaa
     6181 tatcttcaat cgtcactaag ccgcttgtcc ctccgtattc atcttttaag atagcaatat
     6241 gcttaccctt ttttcgcatg atcgtcaata atttttttat agggatcgtt tcagttgtta
     6301 cgatcggctc ttcaatcaac tgttctactg tcagctggtc atctttataa gattgcttga
     6361 tcaaatctgg caaggtgaca taacctaaaa cattgtcttt tgattctaag ataaccggat
     6421 agcgagtatg tccttgttca atcgaagctt ttatcgcttc tttgacggtt tctgaagctt
     6481 cgaatacttg catggacgta cggtctactt gtatatcttt tgccaaggta tcatcaaatt
     6541 caaacacgtt ttccaaataa taatattctt ctttggtcaa ctctcctttt tcataagaat
     6601 tcaatgcgac taatgtcagc tcttcttgag acaatgtctc ttctccttca gatacgagac
     6661 gcacacctaa tagcttccct agccctgcag ctgaatgatt caatacccaa ataaaaggaa
     6721 acattacttt ataaaaataa tgaagtggtt taactacgaa caagacgacc ttctccgtat
     6781 tgacgatact atagctctta ggaagcagtt cgccaataac cacatcgata tacgtaacta
     6841 aaataaatcc cagcgccaca gagattgtac tgctaatagc cgaactaaaa ggtagtttac
     6901 cgatcactgg ttctagtaaa gccgcgacag atccttcacc gacccacccg atgatcaaac
     6961 cggttagcgt aattcctaat tgactagctg ataaatagtt atctaaatgg tgtgtaacat
     7021 gaagagctag cttggcgttc tttacatttt ccttttctag ttgttccaat cttgatcgtc
     7081 tgattttcac taaagcaaat tcacttgcta caaaaaatga agtaattgca atcatcaaaa
     7141 ataaaataat gaaattgata accatattct cccgcctttc tctttttcta ttgtaccctt
     7201 tttctacaaa aagattgaaa ataatgcgta ttcatattta ggaataaaaa aggagatgcg
     7261 gcatttttca gccacatctc cttatcactt caaaatcatt gcgttacttg ttgctaaaca
     7321 gctttttcat gacctctgat aaaaacggta ttcaaaagct tgcatctacg gcattaagcc
     7381 caaacagatg aaaaacatga gaagctgttt ttatctgctc gttcttactg cttgttgcag
     7441 tacagctttt tcatgacctc tgataaacgg tgttcaagta gctgacattc gacattagtt
     7501 caggtcaaag cgctactttc acaaccgctc tttcaaccat gttttccgag tatttccaat
     7561 gataagcgga acagtttcat gttttacttc gctgtactct agcccaaacc atgtttctgg
     7621 cgaggtagcg atttttttga ttgccagttt tgcctgcatg atttgacgtt gccattttcc
     7681 tagagttgtc acattggaaa ctacttcatc aatcaggaca aattggaaat cgccaacttc
     7741 tcgccctggt gtcagagagt atctttgtgg ctgttttggc aaacggcctt gtttcatcaa
     7801 atcatggatg acttggcgaa catatacgtt gatttcctgt tggaccctga aacctaagaa
     7861 cagattcact tggacaataa aatcagtgtc catcatatct acttgatatt ctttcgtata
     7921 tggctcatcc gtcacttcca catgaacaaa ccaatagact cgtgctcttt taggccgttt
     7981 atctaaaatc gaatagatga actgtcggcc aactttatct tcagccatat caggaaccaa
     8041 gaagaccaca tttgtctgat agattggtaa agaagtatca tcacgtaatt ctctaagctg
     8101 cgggatataa tctctcaaag acacatcctc ggttgacgat tcgcgaataa tgttcccttg
     8161 ttcccaaata aacatcacga ctaagatgat caaaccaatc agaacggcta caaaaccgcc
     8221 tcggaagaat ttgacaacac ttgagataaa gaaaacgact tctatcgcac cgaagaaaaa
     8281 ggtaactaaa tatgcaagcc aacgagaatg cccattttgg ataagataaa agtaaagcag
     8341 tgctgttgtc atcaacatcg tcacagtgat cgatagaccg taagcagctt ccatatgatg
     8401 agacgttcgg aatgtgacaa ccactagaga acaggcgatc cataaaatca gattgactgc
     8461 tgggatatac atttgcccga tactgttccc aggatacatg atctttaacc gaggcagcag
     8521 tttcaacttg attgcttctg aaactagcgt aaacgaacct gtaatcaatg cttgcgaagc
     8581 aatgactgca gcgactgttg caaaagcaac gccgatcacg atccaacttt gaggcatcat
     8641 ttggaagaat ggattcaggt tcttgatttg ctgtgcttca gggttttgat aaacattcaa
     8701 caaccacgca gcctgcccaa aataattgat aaccaaacaa attttgacgt aaggccagct
     8761 cagacgaata tttcgtttgc cgacatgtcc aagatcggaa taaagcgcct ctgcacctgt
     8821 cgtagccagg aaaatatttc caagaatgaa caagcctaat ttgttatctg cactaaaaag
     8881 cagctggata gcataataag gattcagtgc acggataact gtccagtctt gactgaagtt
     8941 cattaatccc attacgccta aaaaagtaaa ccaagcgaac atgattggtc cgaatgcttt
     9001 tccgacaata tctgtaccaa agcgttggac catgaataaa acaaaaataa taactaaagt
     9061 gataatgacg atcacgttct ggttattacc gaaccggtca aaaaactgcg gaattcctcg
     9121 caagccttca atcgcagtcg ttaccgtcac agcaggggtc aataccccat ctgctagtaa
     9181 ggcagctcct ccgatcattg caggaatgat caaatatttc ccaccttttc ggacaagtgt
     9241 gtagagtgag aaaattccac cttcaccatg gttatctgca ttcaatgcaa tcacgacata
     9301 tttgattgtt gtcagtagag tcaatgtcca aaaaatcaat gagactgatc cgataacaaa
     9361 atcaggattg attcctgaca atccgccatt atcttcaaca atcgatttca taacgtaaag
     9421 cggacttgtc ccgatgtctc cgtagacgac tcccatcgct acaagcaggc cagtcattga
     9481 tatctttttg gtattgtgcg ttgttgccac gtttttccct catttcttgt tatatggata
     9541 tcctttaggt aagcttgaat gatagatgat attcttatcc atgacacccg ctttcctaaa
     9601 ttgcttctaa ttatccaagt aacacactga agtgtcaact tatttgaata aaattattct
     9661 ttctttggac gttctctgat acgtcttgcc acaaatgaat aaagagcaag gcaaaaaatt
     9721 gcacccaata atgctggtat caaataaaaa ccgccgagtt ccggtcccag tggtcctagt
     9781 aaaaattcgc cgagccaact acctaaaaaa ccgataatga tatttccaga tacgcctccc
     9841 gggacttcct ttcctaaaat tgcgccagct atcaaaccta atataccgcc gataaataac
     9901 gaccataaaa aattcagcat ctcctttccc tcctttatcc atatttctca cttttatttt
     9961 acgctttatt cagaaggact ccaaaaatat gcgccaagaa actccagaaa aaaagaagtt
    10021 gtaacaaaag tgttcagctt atttccgtag aagctgcttc tggaacaccg tgaatcagag
    10081 gttgtgaaat cagcggtttg acctgagtat tagagaaaca aacggaaaaa tagttcctca
    10141 tattttgccg ttttttgagc taatatcgag ggacagctga ttgaacatcg tttattggag
    10201 aggttgtaaa aaagctgttc tgcatcaagt aataagaaca gccaaataaa aacagctcct
    10261 aatgttttta acttggctgg acttattacc gcagatgcaa gcttttgaac accgtttatt
    10321 cgaaaaaaaa ggactgtgac acggcttttg tcacagtccc cttttttcga ataattattg
    10381 tccccctgtc accttcacga tcacaagtac attattccta tgaattaaat tatttttctt
    10441 catttttcgt tgaaccatct tcgttcaaca ttgcttttct tgacagattt acgcgtcctt
    10501 gtttatcgat ttcagttact ttcacaagga tctcatcacc tagtttaaca acatcttcga
    10561 ctttattgac acgatcgttt gctaattggg aaatatggac aagtccatct tttcctttga
    10621 tcaagttgac gaatgcaccg aatttttcga tacggacaac tttccctaga taaacttcac
    10681 caactttgac ttctttggtc aaatcttcga tgatcttgat agcttttttg atcatttctg
    10741 catcagctga agcaatactt acttttccat cttgatcgat atcgattttc acaccagttt
    10801 catcgatgat tccgttgatc gtatcgccgc ctttaccgat aacatctttg attttagcag
    10861 gttcgatctg gatcatttcg atttttggtg catattggct taattcttca cgtggagcag
    10921 caatcgttga agtcaattct tctaagattt ccatacgtgc ttgttttgct tgagttaatg
    10981 cttcagttag gatttgttct gtgattcctt gaatcttgat atccatctgt aaagcagtga
    11041 taccatcttt tgtaccggca actttaaagt ccatatcgcc aagatgatct tccagccctt
    11101 ggatatcagt taggatcgta tagttttcac catcagaaac aagtcccatc gcaataccgg
    11161 cgactggcgc tttgattggt acacctgcat ccatcaatgc taatgtgcca gcacagatac
    11221 ttgcttggga agaagaacca tttgattcca atacttctgc tactaaacgg atcgtataag
    11281 gaaattcttc ttctgaaggg attacttgtg ctaacgcacg ttcacctaat gcgccatgtc
    11341 cgatttcccg acgccctgga gagcctgcac gtcctgtaga accaacagaa aattgaggga
    11401 aattgtagtg gtggatgaat cgtttgctga cttccacccc taaaccatca atgatttggt
    11461 gctctcctag aggtgcgagt gtacaagctg ataaagcttg tgtttgtcct cgtgtgaaca
    11521 aacctgatcc atgtacacgt ggcaataagc tgacctctga tgacaatgga cggatttcat
    11581 ccaatttacg cccgtcagga cggatcttat cgatggtgat caattcacgt acgacatctt
    11641 tttcaagatc ttctgtgatt tgtttaactt ctttcaacaa gtgatcttca tcttcatgac
    11701 ctgcaaattt ctcagcataa gcttcgcgga tatcgatctt cacctgctcg atattatctt
    11761 cgcgagcaag tttttcttca gtcattactg ctgctttcat tgtagcgtaa gaagcatcaa
    11821 agatttcttt tttcaatact tcatcaactt gcagtaaagt cacttccatt ttttctttcc
    11881 ctactgcttg aacgatttct tcttggaaag caactaactc tttgatagcg tcaaaaccaa
    11941 acagcaatgc accaagcatg tcttcttctg aaacttcttt cgcgccgctt tcaaccatat
    12001 tgatggcttg tttcgttccg gcaacagtca attcgatatc tgtttgttct gcttgttcta
    12061 ctgttggatt caagacgtac tcgccattta cacgaccaac ttctacgcct gcgattgggc
    12121 cgtcaaatgg gatatctgag attgatagtg ctaaagaaga acctagcatt gctgccatag
    12181 ctggcgagca gtctgtttct acactcatca cgatgttcgt tacttggact tcattacgga
    12241 agccttctgc aaacatcggg cgaattggac ggtcgatcaa acgggcagtc aatgttgctt
    12301 ccgtacttgg acggccttct cgtttgatga aacctccagg gattttccct gctgcataca
    12361 ttttttcttc ataattgatc gttaatggga aaaagtctgt atcttttgct tctttagagg
    12421 ccacagctgc acttaagact actgtatctc cataacggac taaaacggct ccgtttgctt
    12481 gttttgctag ctgaccgact tcgatttgta agggacggcc gccccatgtc gttttgaata
    12541 cttgtttttc tgacataact tctccttttc cttctcgtga aagcaagacg ctactaaaca
    12601 aatgaaaact gccgaaagcc gtcttttgag cagctttcat ttggttagta ggagtccatc
    12661 tagcttgatc acggaatttt acttaattga tatacgagta aaaaaagtga gattcatgtg
    12721 aatcccactc tttttgacta aaattaacga cgcaagccta aacgtttgat caattcgcgg
    12781 taacgttgaa cgtcagtttt acgtaagtaa gctaacaagt tacggcggtg accaactttt
    12841 ttcattaggc cgcggtatga atgatggtct tttttgtgca cgcgtgcatg ttcattcaag
    12901 tggttgattt cttcagttaa tacagcgatt tgtacttctg gagaaccagt atccccttca
    12961 tggcgagcgt attcgttgat gatttcattc ttgcgttctt ttgaaattgc catttctttc
    13021 acctctttcg attattcccc tgcgactgag taaaacgttg gtgattcgct taaccaagtg
    13081 gcaggtcatg tgcctagagc acacagctat actttacctg aaaccgggtt gaaaatcaag
    13141 tagttaatca ttaagaaaag gtagttttca aacaatctta ctactacttt tccatttttg
    13201 tggtaaacta taatgcgaaa atagacttga aacacagtct tttaagaaag gggtacatat
    13261 tccatgatta caatggatga tatcattcgt gaaggaaatc cgaccttacg cgaagttgca
    13321 aaagaagttt ctcttccatt aagcgaagaa gatatctctt taggaaaaga gatgcttgag
    13381 ttcttaaaaa acagccaaga tccaatcaaa gctgaagaac tacacttacg aggcggtgtt
    13441 ggcttagctg ctcctcaatt agatatttct aaaagaatca ttgctgttca cgtaccaagt
    13501 cctgacccag aggcagatgg accttctcta agtactgtta tgtataatcc taaaattttg
    13561 agccattctg ttcaagatgc ttgtttaggg gaaggtgaag gctgtttatc tgttgatcga
    13621 gaagtaccag gatatgttgt tcgccatgcc aaaatcactg tgtcttatta tgatatgaat
    13681 ggtgaaaaac acaagattcg tttgaagaat tatgaatcga tcgtcgttca acatgaaatc
    13741 gaccatatca acggtgtgat gttctatgac cacatcaacg atcaaaatcc atttgcacta
    13801 aaagaaggcg tattggtgat cgagtagaga attggcaggt tccttcgata ctatgctaca
    13861 agaaagcttt ataaaaaagg ggagtgctca aaagctgcac ttcccttttt tatgtttctc
    13921 cactaatcga aatttagata gacatctaga aatcgcaatt ccaaaaaatt ctgtgtattg
    13981 gatacgcatc ttttcttatt ttttcttatt tcttccatat gggtgtttat ctcagagatg
    14041 atagaatagt ttctaggata aaaagtagca gctacgatgt tatccttcgt agaggattcc
    14101 tgcttcagct agtttttgtt tgatgttttt cagcatatgc ttttctgggt agcggatcgt
    14161 atgcagatgg atgccgcctg ttaattcgga taacaaagta gcattacttt tttgataacg
    14221 tctcataaag gaatcgatat cttgtggcgt tttgatgtgc aaagttcctg ctaactcacc
    14281 gtaaagggga tgttccacaa ttacatcaat gatttcacca ccattttcaa cgattagtct
    14341 cagttcttct tctgtttgat cttttccatg ctggacagcg attttactga tgtgcccagt
    14401 attggtttct tctgtttcga ggatataacc tcttgctgtt gccacgatac ttttacctgc
    14461 agctcgtagt aaagcaatat cgcctacaat gatctgacgg ctgactccaa aagttttcgc
    14521 aaacttgcta gcactcactg gtttttctgt atttgccaag gtttgcatga tttgttttcg
    14581 ccgtgtctct ccttcgatca cacttattcc tccttcattt tgttttggta ttcgcgcgag
    14641 tgtttcatca aatcaacata cgtgtctgtt attgtttcct tatatccctt atttttcagc
    14701 agactaacaa ctttatccaa tacttgctgc tctctttttg catcataaac tgcttggttt
    14761 tcttcttttt tcacttgtcc tattttagtt accgtatcca ttcttttttc taataaagca
    14821 accaactctc gatcgatcat atcgatctta tgacgtaatg tttcaagaga ctcttctgtt
    14881 agttcttcct ctactgtctt gatgatacga gcaggatttc ctgcaatcac tacattgtcg
    14941 ggaaaagatt tggtcacaac cgaacctgcg ccgactacta cgttgtcccc tagagtgaca
    15001 cccggcgtga cgataactcc tccgcctaac caaacattat taccgatttt aatgggtttt
    15061 gcgtactcca gaccgctgtt acgtttcact ggatgtagtg gatgcgtcgc cgtgtaaagc
    15121 tggacattcg gagcgaacat gcagttatca ccgatttcaa tcgtgctgac atctaaaaat
    15181 gtacaattga aatttgcata aaaattctcc ccaacaaata tattatatcc gtaatcaaaa
    15241 ttgataactg gctccatata gatttttttc cctgtacggc caaaagtctc tttcaacaat
    15301 tgggatcgca gttccgctga ttctgcttgg ttgatgattt ggctttgact tcttgcaaac
    15361 atacggtcgg caaccagctc aggatcattg gcaaaataaa gctcgcctgc aatcattttt
    15421 tctttttcgg ttttgaattc agtcataatc tgctcctaat tcttgtattt ttttgaaaac
    15481 aaccgctccg tcttcttgaa taaacgtata aggctccgct aaataaacag gcgtttgatt
    15541 gattactaag attttggctg acggctggcg aaactgtacc agatcacaaa acggatgaac
    15601 ttgaaaactc gttccaacaa tgacgatcag ctctgcttgg ctgacggcat aagccgcctg
    15661 ctccatgact ccatcttgaa acccttcacc atataacacg atgtctgggc gtatttgtcc
    15721 tccgcatgct tgatgtttgt cgctgtgaag atattctttc cagtctaccg tttgttgaca
    15781 ttttcgacaa tgacattgat acagactccc atgaaaattg acgagttgcg ggcttttagc
    15841 ctttgcgtgc aaaccgtcga tgttttggga aacaacccaa acattttttt ctttcgctag
    15901 atgtgctatt tccaaatgaa tgatattggg ttttgcttca ggatgataaa tctttttgac
    15961 aaattgatag aatttatccg gttcattcac cattgcttga tgactcagca agtattccgg
    16021 ctgctccatc ccgtggtaga ttcctgacaa agatcgatag tctggcactc cagaaggtgt
    16081 ggatacccct gcaccggtca aaaatgtaat ttgctgataa gctaatatct cttttgctgc
    16141 ttcgtctact gttaattgtt tcatgttttg tcacctcact ttccattata gcccatactc
    16201 gaacaaattt attgttgaaa agtcagaaaa atgggaataa aaaagtgtta tgggcatttg
    16261 agcaatttat cacaatgttc actgacaaaa gaatgaaaac gtgttacaat actgctgtat
    16321 aaaagtgagg tgaatcgata tgttaccatt ctttatttat tggggcgtta ttctggcttg
    16381 tatcgcctgg ttagcgttaa gcgtttattt ctctgttttt tatcttgtac gcaaagaaaa
    16441 tggaaatctt tgggcttttg ctttattcaa cgtgattgcc gcaattgttt tagccattac
    16501 attagccgta tatcgaacat ggggctgggg aatcacccaa tactcaagtt tgatttatct
    16561 aatattagcc atttatggcg tcgttgttct tttgcaagcg atcctaggac gtgagccaaa
    16621 gaaagcggca gcttaaatga tcgagatgga actggacaca ggaaattttc tgttctagtt
    16681 ctttttttat tgtattacat acatttttgt gtataattat actatcgttt gcaatggata
    16741 gatgattcac attttgattg ttttttcggt aaaagtaaag atttctatat ttatcagtat
    16801 tttttcaaaa accagcagtt atagattgac aaagcctctc gagtatcgta aacttttttt
    16861 gtataaataa ctgaatacga gatgcctcaa tcaatgaggt agaggtcgcg agaatcatta
    16921 accctgtgga gtgaagcaaa catggagaag caggaatagg gaggatcgcc gaaatgtgca
    16981 cttttttgct tggagtgtat gttgggacgc aagttaagag cttgcggact gtcttagcag
    17041 tgatgctaag ttgcgctatg ttttgtgaga ggtttatcac cttatggaat tttagcggcc
    17101 tttttatatc tataaatcgg tcgcttttta ttttttcaga taaagcttta ccgcatttca
    17161 tctagaccgc tcgtgtatca tataatcaga aagggagaaa aaaatgaaaa aaaatatggt
    17221 ttcagttgcc gtactatttg cttttgtctt ctcactattc agtgtgggat ctctggcaaa
    17281 tgcagatgaa aaaacaccga gcgatgagtt tcgcgtaggt atggaggctg ggtatgcacc
    17341 cttcaactgg tctcaacaaa cagatgagaa taatgccttg ccaattcaag gccaaaatag
    17401 ttacgccggc ggttatgatg ttcaaatcgc taaaaaagta gcagatggtt tagggaaaaa
    17461 attagtcatc gtccaaacga aatgggatgg tctggctcct gcccttcaat caggcaagat
    17521 cgacgcgatc atcgccggaa tgagccctac tgctgaacga cgaaaagaaa ttgattttac
    17581 cgatccttat tacgaatcac aattagtagt agtagttcaa aaagatagca aatatgcaaa
    17641 agcaaaaagt ttagcagatc tttctggagc aaagatcact gctcagttaa atacattcca
    17701 ctatactgtg atcgatcaga taccagatgt gcagaaacaa caagcaatgg ataatttctc
    17761 agcaatgaga acagctttag cttctggaat gatcgatggt tatgtcagcg aacgtccgga
    17821 aggtgtaacg gcaactagtg tgaacaaaga cttggaaatg cttgaattca gcggtgcaga
    17881 tggcttccag acaaacccgg aagatgttca gatttctgtt ggtatgcgta aaggcgaccc
    17941 ggatatccaa aaagtaaacc agattctttc tggcatctct aacgatgaac gaaccaaaat
    18001 tatggaccaa gcagtcaaag atcagcctgc tgcaactgat agtgaagaaa aacctggcat
    18061 catttctgat ttcactacga tttggaaaca atacggcaat atgttcttac gaggtgctgg
    18121 acttaccttg ttcatcgcat tgatcgggac agttgtcggt actacacttg gattactgat
    18181 tggtgtcttc cgttcggtac ctgaatcagg aaatgcactg actcgcttct tccaaaaaat
    18241 cggaaatgca cttctttcta tctatgtcga agtattccgc ggaacaccga tgatggtaca
    18301 agcaatggtc atcttctacg gtctggcact cgcttttggc atttcattga accgaacgat
    18361 tgccgctttg tttattgtct ctctcaatac tggagcttac atgacagaga tcgttcgtgg
    18421 ggggattttt gcagtagatg aagggcagtt cgaagctgcg caagccattg gtatgaccca
    18481 tggccaaacg atgagaaaag ttgttattcc acaagtttta cggaatatct tgccagcaac
    18541 aggaaatgag tttgttatca atatcaaaga tacagcagtt ttaagtgtaa tcggcgtagc
    18601 tgatttgttc ttccaaggaa actctgcttc cggtgcgaat ttccaattct tccaaacatt
    18661 tacgatcgtc gggatcatct acttagtcat gacctttacg atcaccagaa tcctgcgggc
    18721 ggtagaaaag aaaatcgatg gtccttcagc ttataccaag cttgaagaaa tcgacaattc
    18781 aaatttgcag gaataaggag gaagaatcat gaatgaaaaa attattgaaa tcaaacatct
    18841 aagaaaaagc ttcggtgaaa atgaagtatt gaaagatatc agcgtaacag tcaataaagg
    18901 tgaagttgtg acgatcatcg gttcttccgg ttctggtaaa tctactttct tgcgctgtat
    18961 caatttactg gaaaaaccaa caggcggcga gatcatttat aatggagaaa atgtattagc
    19021 tcctaaatat aatttaccta aatatcgaac gaatctaggt atggttttcc aatccttcaa
    19081 cctgttcaac aatatgaatg tcttggaaaa ctgtatgtct ggtcaaacaa ctgtcctgaa
    19141 acgagataaa gaaactgcca aaaaagtagc aatcgaaaat ctggaaaaag ttggtatgga
    19201 tcgttatatc gaagccaaac cagcacaatt atctggtgga cagaaacagc gtgtcgcaat
    19261 tgctcgggca ttatcaatga atccagatgt attgctattc gacgaaccta cttcggcgtt
    19321 agaccctgaa atggttggcg aagtgcttaa tacgatcaaa gacttagcac atactggctt
    19381 gacaatgctt atcgtgacgc atgagatgga atttgcaaaa gatgtatctg accgtgtcat
    19441 ttttatggat aaaggtgtga ttgcagaaga aggtacacca gaagatatct ttgtccatcc
    19501 gaaagaagaa cgtacaaaag aattcctttc acgtatttta aatgcttagg atcaatagct
    19561 gattgggaca aagagctgcg gtgtttaccg cagttctttt ttatgaatta ctcataaaag
    19621 gtaaatttct gaaaattttg gaatcttagt tgtgtttttc agaaaactct cttaatatgg
    19681 aagtaattaa acttttttag ttaggatgat ttaaaaatga atgaagagca agaactgcag
    19741 cgtggactga aaaatagaca tgtccaactg atttctattg gcggagcgat tggaacgggg
    19801 ttgtttttag gttccggaaa gtctatccag ctagctggcc cttcgatatt gctcgcctat
    19861 cttattactg gatgcatctg ttttttcatt atgcgtgcgt taggcgaact tctgttatca
    19921 aacaccaata accattcttt tctggatttt gttgcagaat atcttggcaa aaaagcggct
    19981 ttcatcactg gctggacgta ttggttctgc tgggtatcga ttgcaatggc cgatttaaca
    20041 gccatcggaa tgtatgtacg ctactgggcg cctggtgttc ctcagtggct tcctgaactg
    20101 attgcattag gtcttcttct ttgcttgaat atggtggctg tctcattgtt tggtgagtta
    20161 gagttttggt ttgcattgat caaagttgta gcgatcattg cctttatcct cgtaggagct
    20221 tatatgatcc tcacccacta tacgacagat attggcacag ccagtatcac gaatctatgg
    20281 tctcatggcg gattctttcc aactggtgca aaaggattta tcttagcttt ccaaatggta
    20341 acctttgctt ttgctgggat agaattagtt ggattagtcg ctggagaaac agaaaatcct
    20401 gaaaaagtgt tgccggaagc tatcaataat attcctatca ggatcatctt attttactta
    20461 ggatctcttt ttgtcattat ggcaatctat ccatggaaca gcttgaatcc agataacagt
    20521 ccatttgtag aagtattttc ggaaattggg atcacgattg ctgcttcctt gatcaattta
    20581 gtcgtattaa gtgcagcagc ttctgcctgc aacagtgcaa tatatagtac aggacgtatg
    20641 cttcgttctt tagcgcaaga aggcagtgcc cctaaaaaat tcaaaaagtt aacaacacat
    20701 catgtgccag ggaatgcttt gacattttct actatcgtca ttttcatttc tgttatcttg
    20761 aattacgtga tgcctagtga agtattcaca ctagtaagca gtattgccac tacttgtttc
    20821 ttgtttatct ggagtatgct tgtctacacg cacatgaaat atcgtaaaag tatcctcggc
    20881 aaaaaagctc attcatttaa aatgccgttg tacccattta gtaattatct ggtatttctt
    20941 tacatggcct ttgtgtgtgt cgtcttgttc ctaggaaaag atacgagaat cgctttatta
    21001 ctaacacctg tgtggttctt gcttctatta ctaatctatc atatgaaata tgaacgtaag
    21061 aagtaagaaa gtagttgtga cagaagtggt cagcttcgag aaataagaag gaatttacga
    21121 aaattgcttc ttaaattttt gtgaaatttc ggcttatttc ttggagataa aagcttctgt
    21181 cccagcctct tttctatttc cccggaaaca accgctgata ataggcatct gcttttttat
    21241 aggaaaaaat ccaaatacca gatacaaaaa aacgtccgaa taacccgatc aacggtacaa
    21301 aaaagacacc ggcaatcaag cataagttaa tcatcatgga gataagactt cgacggtcct
    21361 ttgacaaaac tctcacgaca tgggaatctg gtttatttgc tttcatgatt tgcctcatca
    21421 agataccaaa agaaaaactc cacatcgtgt atactaagac ataagccatc tcaggccaaa
    21481 aagcttcagg gttacgcccg acccaactag taacagaagg acataaagtc agccaaaaga
    21541 gcaaaaagat attcgaccag accactcggt aattaattcg ttctgccatc tgaaatatac
    21601 gatgatggtt cacccagatc accgcaacaa atataaagct gatggtataa gctaataatg
    21661 tatttcggat gacccataaa gaatgaaagc tagatgtatt agggatcttg atctctaata
    21721 caataactct caaaacaatg gctaccactc catcagtaaa tgcttctaat ctcgactttg
    21781 tcacgatgat tcctcctcag atgattcagc gcttttattt tatcacttac gttattaaat
    21841 aaccatcgtt ttatataggg aattttctga aaattgttgc atatttttcg aaattttgtt
    21901 acttttaaaa
//
LOCUS       DAJSBL010000041        21526 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.041, whole genome shotgun sequence.
ACCESSION   DAJSBL010000041 DAJSBL010000000
VERSION     DAJSBL010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21526
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.041"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            40..300
                     /locus_tag="ONT85_001889"
     CDS             40..300
                     /locus_tag="ONT85_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2378858.1"
                     /translation="MNHEKFIEKCKAIVRERIENEIADLSGAVPEFSIFIVWSCKTLQ
                     NSKALVSASLKGAPYFEITLNGDKGEIYVDTYLKKSNECIKV"
     gene            341..568
                     /locus_tag="ONT85_001890"
     CDS             341..568
                     /locus_tag="ONT85_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2378859.1"
                     /translation="MRNYWYVSLTNEYPRTIGDCSVRVVRSVQIKGKYSIVEMLREAT
                     PNEVDKCKLIYCGHGYWKDEYIQYNIERYIK"
     gene            633..1205
                     /locus_tag="ONT85_001891"
     CDS             633..1205
                     /locus_tag="ONT85_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2378860.1"
                     /translation="MKLKDYIREGYNVVTAPNLAYKIQEDYPNALVFTDRALDAIPIG
                     KLLVDRFYSNNPAVLSAKPLQNAYAIERFTCEFTGYETVVPMTGETGRKVIQQIKKRI
                     AELTLDDGSNTNLLEIKLRDTDSVPEVYYKGQRMDEMPIGLVDITYHWDTEEYECQVP
                     KGANDITIEYYDSHNNKYLDRKIIGHKRDM"
     gene            1206..1517
                     /locus_tag="ONT85_001892"
     CDS             1206..1517
                     /locus_tag="ONT85_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCR2378861.1"
                     /translation="MIDVSSKQARARFYGSTEWRKLRSQCLERDHYECQWCKAEGRLT
                     TQHDSILEVDHIKELEYHPDLALDINNLRTLCKDCHNKRHNRMKYRGQQKKRKWDDEW
                     W"
     gene            1508..1768
                     /locus_tag="ONT85_001893"
     CDS             1508..1768
                     /locus_tag="ONT85_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2378862.1"
                     /translation="MVVRKTANRDDVKRAEAIAMVDKAIDKLVLFKSQLIEGTTTVED
                     ISINNRLIGNANEVTGTFNINVDIDYTLSPFAHIVESNEQDG"
     gene            1929..2288
                     /locus_tag="ONT85_001894"
     CDS             1929..2288
                     /locus_tag="ONT85_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2378863.1"
                     /translation="MADLQERNRLVSAEERRLNKLFKDIDKDKKKVVSGLITQAARLK
                     ILLDEMWIDISEKGDYELFSQSENQKPYERERPVAKQYNSRDQAYQRVIKQLTDYLPE
                     EKREPTKNAALDGSDLL"
     gene            2285..3928
                     /locus_tag="ONT85_001895"
     CDS             2285..3928
                     /locus_tag="ONT85_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HCR2378864.1"
                     /translation="MTTLKPFYFDEYVDLYEKGVIPFNKERIQLITYLKNEVLVRDDI
                     YFDEEMIQKFIKYTEKNFFPLAKYQKFITPFIFCYQKEDDEVFFDEILNSIARGGGKN
                     GFMSARDSFFISPLYGVRNYDVTITANSEKQGKVSFKEVYEMVQMRKLEKQFYLTKMA
                     IVNNVTNSIFSYRTNNPKTMDSARDGCLEFDEIHQFESSDLVDIQRSGLGKIKHPRTF
                     YNGTNGHVREGFYDKMLERAQKIFSGENKNDRLFPFICKLDNIDEMDNPKMWSKANPM
                     FEEDSAYSKRLFSTVMKEYKKLEDEPSGRREFVVKRMNFTEGDAESDITTHEKLMATK
                     QELPNLNGRSCVAGFDYASIRDFATVGLLFKIDDKFIWQQHSFARKKFLDTFKLKAPI
                     KDWEKKGLVTIVDEPSIDPRHLIDWLNEKRKIHQIEIVCADGFRMDLLKPLLEKESYN
                     FEFIRNIRGVQSKVAPIIEDGFANERFIFGDDPMMRWYTNNSFIKEDKLGNKTFLKKE
                     PVRRKTDGFHAFLAALYKREEITDMDYEEAFDMLDEIEF"
     gene            3963..5045
                     /locus_tag="ONT85_001896"
     CDS             3963..5045
                     /locus_tag="ONT85_001896"
                     /inference="COORDINATES: protein motif:HMM:NF028182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2378865.1"
                     /translation="MKKRYFLFVTIFIIIAICVLTVFLPQITEYLNYRIFTTKVDDSV
                     TSLSRLEFIKICASVFGPILTVMVFVNTLNMQKKTEQKQNELEVKQAKAENERAKKEL
                     LTQIDREFYFLLNMFIEIQKDQNAKQAVIALQKLAIFNEKGFNQDYAFTSKDLSDSEI
                     GSTIEQSGPLKGTVEYIKFETFDVSKSMGNYQTKTKDVATSLHDQKVAILNEQYEKMQ
                     IYLGRYFKMFHRIVKTLNEYYDDYNDFDVKRYTKYIGTLRTQISPAEFQVILFNSLYI
                     KRGFGLGIQLIGSGFFGDDFDFETNQHFETSINEQWFLSLSTVDSDNSIKRQKLSEYI
                     KELGSVEYKKIANFESLYKLFNETKL"
     gene            5089..6261
                     /locus_tag="ONT85_001897"
     CDS             5089..6261
                     /locus_tag="ONT85_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HCR2378866.1"
                     /translation="MSLFDIFKQSIRNEEPSEWIPDFVYGEEVSARAYLKIMAKNTVL
                     DFVARTMSTLEVKFKNKDGTSDWDYILNVRPNSDMSAATFWQKFFYRLLDDNEVLVIF
                     TDDNQLLIADDFSRTEYAVYDDVFTNVMVKNFVFERSWNMSDVIYMEYNNEELERFTR
                     GLFEDYAALFGRILEVAMRNNQIRGSVSIDSTGTANDEKGKDGKTRSQRLQEYIDRIW
                     NSFKTNSVAIVPKVKGFEYEEYTNKQGVSNQSLDELNKMKSSLIDDVANAIGVPTALI
                     YGEKAELDSNIKAFRKLCIAPLVKKLQDELVAKIISRQEYKNGERIEVKKVLQKDLLE
                     YATQIDKITSGGAFYIDEVRDEVDYDELPNNEGKIRIRTKNYEETVKGGENDNAES"
     gene            6248..6952
                     /locus_tag="ONT85_001898"
     CDS             6248..6952
                     /locus_tag="ONT85_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HCR2378867.1"
                     /translation="MPKAKKVPFQFTNEIQNGKHILTLSGNVQKKYWRDDDVINAKDI
                     RDSLDTVTDDIVIKLNSPGGDVFEGIEIYNYLKDHPSNITVEVTGLAASAATFIVAGA
                     NDVIMNVGTSLMIHEASTFAWGNKQDIQKTLNALETIDDSILAIYSDKTGQSVDKLRE
                     WMNEEKWFTADEAVKYGFANSVKREEPQNEPQNIAAMIQDAVAVAIANLKEPVTNKIE
                     QESKQKSLIARLRKGE"
     gene            6957..8117
                     /locus_tag="ONT85_001899"
     CDS             6957..8117
                     /locus_tag="ONT85_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HCR2378868.1"
                     /translation="MLKITDKTADAKKAFNAISSKEDATPEQINAALEEYVTAIAEDA
                     GKQVRAEYEELKNVTDNRVLEARGIPTLTAEETKFYNEAVKSGGFDSDVVWPETILER
                     VFENLQNDHPILGLINFTPTVGRVKVIRARRKGVAVFGPLHKDLEGQLDAEFGATEFV
                     QLALTAFFLISNDTLDLGPRWIDRFINLSLSEAVRDVWAKKIITGSGKDEPIGLLKDL
                     DGAVTSGVYPDKASAGTLTFAKEKIVLELAGVMKKLSKYTYKVDGDDEGEVKYRSVAG
                     RVYLIVNPVDYWDIVARVTFANLNNVYGTTMPFIAVDHIIESVDVPESKCIAYVDKEY
                     EATQSRAEKVYVYKETFAMKRATLYAVDMLGNGYPTNNDAAQVYDLDFTAGE"
     gene            8193..8471
                     /locus_tag="ONT85_001900"
     CDS             8193..8471
                     /locus_tag="ONT85_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HCR2378869.1"
                     /translation="MNTEEFIKEYKARFRIFHTSEDEDIGKQLESGFADIKSLIGSFD
                     PSTYEKGKELVYERTRYLRNESLEYFYDNFQTMIMDASIDLAGAGYAN"
     gene            8452..8823
                     /locus_tag="ONT85_001901"
     CDS             8452..8823
                     /locus_tag="ONT85_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HCR2378870.1"
                     /translation="MLDMPIKSNYKKPKIVAGDLNTPVTFFEFQPNKGPEPGDEEKKE
                     LYFCTALVYNPSMKDRDILGTKEGVTIKIRDPHQSYIPTNKHKVRILDYRYGDKVWEI
                     IDVSPDLEENSIVKIVLELIT"
     gene            8839..9243
                     /locus_tag="ONT85_001902"
     CDS             8839..9243
                     /locus_tag="ONT85_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2378871.1"
                     /translation="MSNVKFEGVQETIQALEKKYGEKKTKTLTKKAINVGAEKVEKQL
                     QTDMTVFKDKGYTIAEVVRKNATYRDYKAEAEIGWNGPHQRYRIIHLNEWGYTRKGKQ
                     IRPRGFGVITKSLKNSELLYFDAVGEEVKKSL"
     gene            9240..9650
                     /locus_tag="ONT85_001903"
     CDS             9240..9650
                     /locus_tag="ONT85_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2378872.1"
                     /translation="MKDMLNIIYEALCLNEYIHSMTYNENTEQYRIKFYEQPETADKT
                     GPMITIRPVDTPNEAYHGSNKELSVEHMIQIDVESKYRATCKQIQYEIKKEMKKLGFG
                     QISGQGLDEYFTETNRFVDARRYEGNTKIYDTEY"
     gene            9711..10298
                     /locus_tag="ONT85_001904"
     CDS             9711..10298
                     /locus_tag="ONT85_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR2378873.1"
                     /translation="MTLVGFKKMTIGVFDSTGKIPAANLYVIEGEQNKGATVSAEISG
                     LSKEPSKVYGSNIAYYVSQKGTGDVSATFGLLDLPTEVNDKILGYKTDTNKISFLGED
                     TEPPYCAILMESEDLNGDTAMLAMFKGKFSRESINLNTTTNEAFEPEAEEYVFSAIAN
                     DVEGDAKGQTVAKYVGDEEASITGLKEMVFPAAGE"
     gene            10371..10838
                     /locus_tag="ONT85_001905"
     CDS             10371..10838
                     /locus_tag="ONT85_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig domain-containing protein"
                     /protein_id="HCR2378874.1"
                     /translation="MPDTFKIYKKNGTEFTKVAEGESPLSITGIAANTQVAKGDYQAT
                     RVVGGQESVKVDIPAFTTLPISVTGVTLDKTTAEVEQGGTLKLTPTVTPANATDKTGS
                     WSSSNTAVATVTGGNVTVKTDAEVGGTTEVSFTTTDGGKVAKCTITVIEKAAG"
     gene            10884..11192
                     /locus_tag="ONT85_001906"
     CDS             10884..11192
                     /locus_tag="ONT85_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2378875.1"
                     /translation="MAKVRIELKEESGKKVYENIDTTGKDYRKALETIKKLNEDGVMI
                     WNRLDIYLDFAVGIFRDSKLTAEQILDGLPSEKVMSTLDDILGEVMGIESNPDPDAKK
                     "
     gene            11387..18121
                     /locus_tag="ONT85_001907"
     CDS             11387..18121
                     /locus_tag="ONT85_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCR2378876.1"
                     /translation="MANGKPLGNMIIKLDLDSSAFSKGLAGAKNAVNHQMKAMKSQMQ
                     VMNSSGNSLGALQAKYNGLGGVLQANEKQVELLTKAYKDSFDSNGNATASTAKYANQL
                     NQATARSASYESQMKTTVGQIARMKVETEGFTGKLKANSDVLINSGKKIEAFGKGVSG
                     IGSALTVGVTAPIVAGATAVTSAAISWESAFAGVKKTNDEVVDSNGNVVYSYSDLEKG
                     LRNLATQLPGSHQEIANVAEAAGQLGIKTENVVSFTKTMIDMGESTNMSAETAATSLA
                     RFANITQMSQKDFDKLGSVIVDLGNNFATTESEITEMGLRLAGAGKQIGMSQGEILGF
                     ATALSSVGVEAEAGGSAFSKVMIQMQLAVEKGFGAFDQLKQMAEEQGVPWVNLVNAVR
                     DGGKSLKAVSEQMGFTSSDLKKMYKEADNSKSSLENFANVAGVTSEQFQKLFKSDPSE
                     AIIKFIQGLKDSEKHGTSAIKVLDDMDIKEVRLRDSLLRAANASDIFKGAIEKGNGAW
                     KQNTALTNEANKRYETTESKLKILKNEVIDTAIDLGGPFVDALRDGLNVSKPLIKNLG
                     NMAKAFSAMSDDQQRNIIKWVGLAAAAGPALKLLGTGTSVIGKVTSTTGRLTKSLVDL
                     AAKAAEKKAMDAFASSVTTAGAAAAKTAGAGGIGGLVPMMGKTASAASTAAGASGIGA
                     MTTSLGLLGPALLGIVGVGGALAVGYGAWKLFGEEAWNSSQRVKQWGTDVGEEIDSTL
                     DGVQKNIEGANGQFGLLKEGFTVDDANNMAANFATAGTSLETSLTGRIKALDDALKGL
                     PQSVQDAMKEVAESEEETLGQSLSKIKDNNEEIKKIRERAANQNRDLTVSELQQIQDL
                     MKGTSQAYVDTLDVSAKDRKKILSAMNGDVAQATEEEAKTWLQSLAKQKAATTENYRQ
                     MLEEKKAALKEKGVSKEFIDALEDEMNDYIKTTNQGFDSQIAAITNKYPQLINEVSLS
                     NGQLFSKTAAAMDNTGQYTKVFLESNEKILAQAKKTSDEIADNAEKNAEQLGMVADEA
                     THAGSTWNGLVLDEKTGKVKTNVQEVVNEATKDFTKWNELKPVVHDAKLTTNAKDVIS
                     IAAVQNGYWDDMEWKEKEALLEDECSKNVYKALENAGKWNELDIPSKEAILTSNTPEV
                     MGETLMNLGLWDTYKLQVKDLDLNAYKVYDAIDGSEEKLNSWAQLDNPTKELLLDNSD
                     FSTKLFNSEEMFKRWNQLSSNAKFLSANNTKFTEVLLSSDRMWNQWVNLPDNEKRLLA
                     DNEDLATTVLSSEEIYGRWTQLPDNQKYMLADNTDLANKIFASEESWNAWKAIPDTVK
                     NLLGNNIDLLAKVKDGTVSVEEYNKVMPLLKTLYGDNVNLKEAISGAKSSIENYNKNI
                     FPSDKTAVGHDEASQAAKDAFEAFNIFQTKIPDKITKTVSADFIGPMPKNAKGTNFHP
                     GGAAMVNDQKGPTYEELITLPSGETFIPKGRNVVLDLPRGSKVLNATKTKRLVPKYAD
                     GIGNITTVSSTLNLDALILAINELTTVLRTQLMAGTTDAKDTKLSDETKVADPIIPDS
                     LSEKSDQYLGIGAQWLTNLMNGWNSAVPQYMNSEMVFITNYLNALKLQNNPNYTQGAT
                     WNKNLLNGWNSLTGTFIATINSFCNQAMVTLRNYNTPMYNNGRTWQQNNLNGWNSLYG
                     SFIARVNQLGNDSINNLRSKSGGFYNAGTFLLQSLINGMNSLGNSLSTTMNNVANTMV
                     GGIGKGVNGVISGVNYVLKEVESSKNIGNWPIPQYAKGTDGHPGGLAMINDQKGLVHE
                     EYVQMPDGRSFIAKGRDLLVNLPKGAQVLNASLTKKLKERLNIPHYEKGIGNLDIVDL
                     LDDEKRMLEFLTSKVDFSGINEPWLDMTKSGTSLMSKAANTMLQSKLSEFFTHGNFDG
                     AVNANGVYQYLVDVAQKVMGKFPGLTVTSGYRAGDAYYHGKRQAIDLAYPGISGDPRY
                     TAAANYAFEKFPSKIAYVITNGRVRDRMGLSGTRSSGQWTNWPDGDHFDHIHLNGSMG
                     SGDIFNGGVGGSGVARWRSYVSKALKMNGLPATAAYINAWMSQIQTESGGNEKAIGGN
                     DGLAEGNATGLLQTKPGTFAANAFPGHGNIMNGFDNMLAAINYAKKRYGVAGMLQVIG
                     KGHGYANGGLITKDGLYRAGEGNKPEMVIPLTRKTRAIELMGQALAFLSGSNKKETKS
                     EINSVDNRHILQAIEKQSRMTNDLLSLLLSFFKSNNRSDKELALDIQKILIRRT"
     gene            18125..18997
                     /locus_tag="ONT85_001908"
     CDS             18125..18997
                     /locus_tag="ONT85_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate protein"
                     /protein_id="HCR2378877.1"
                     /translation="MRTVILRNKLNEEIDLSTEDYFATEMSNLGFEVTKEHIGQWGSF
                     REIGESIELSEFQSSVMISVHGFREQQLYGSLVEFLANGPFELEFSFDSETMIRKCSL
                     KSLSKTEIDSQSSLLTDTLNLYFTSSWYSIKREKLIQNSTSLNTRGKVYSFVRPYVYT
                     KNVWEKKGVFKFSNNSVYLTNSTERMSPLKIRIIGECSNPYWEVIQNSKVVASDGYFI
                     NMKDTQILEVSSLLEDQTAILKDVGGVESSVYQQQDFTKSNFVQAPLGEFSVVFHVGH
                     ANVEIELYEERDLF"
     gene            18994..20043
                     /locus_tag="ONT85_001909"
     CDS             18994..20043
                     /locus_tag="ONT85_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2378878.1"
                     /translation="MILAITLFHRDMNLYNERLISTEFEFGTDEIGEEVSNFITDKYV
                     HVKSGDFLLAKFIPSGKIAYFGVISSQEDKKINCKGLLSLADSEIPTVKISGANYEEH
                     IRRLIEYYLLNDPTKQLKNILEVKTESVTSHSYQAVDTNKRRLNAYIINGFKKYNVKW
                     YVKEIKGKKIYTGIRAVDDSIYIKDNSSEFNDWDVFVQSPGPGNENKLLIVDKAMKDI
                     ENPLVLSTWYLDDENNLTQDSTNKNIALPTVNLVNIYDQTQEDRPSYEEVAKSELKGN
                     AYSHEIKVNVVLNARNINVENIETGMLATIVYKEQTYKSVLTAWRISSSKKNIELTFG
                     NVRSRFMDYFEDNGG"
     gene            20047..20871
                     /locus_tag="ONT85_001910"
     CDS             20047..20871
                     /locus_tag="ONT85_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2378879.1"
                     /translation="MVSNVDGYQFENVKVSAENDARLYHVLYNRKNQVIDGYDQSMNL
                     SSSGLTVKVAAGAAIIQGRMVVVRQEESITVPANSSGYIALTVDLTQEVIPGSILPES
                     EEYEWTNNQVKLEFITKVIKGNLNNGDKVYNLPLCSVNSTGSTVSISKISDSYELTLS
                     KGEILWRGTALMHDTQTVQPSKKIWQTVSGFLLVWLPYENGQAIEDRYVTTPFYKERI
                     TFTNVLGEIVSGFDDYHKKWFSKRINYNSNTNIFTGAASNASGDNANMVLRYIVSF"
     gene            20889..>21526
                     /locus_tag="ONT85_001911"
     CDS             20889..>21526
                     /locus_tag="ONT85_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HCR2378880.1"
                     /translation="MANLEIKLSANKRQPYLRHRVVGRVGDGGLTTINVQLLEEDEIT
                     PFVINLNGTLKFVGEVSNGNYTEGEPEIIDSTNGLISYTFTKSNFSTSHQFKQAYFEY
                     VDPNGKKVTFQNFIIDVLERVDINSEQAKYYISSLEKLQSEMQTTFNQFISDKQVQYD
                     QIYSKYNELVRLINESDKQVNDRIDKANNRIDQTNQQIGDLGKLKKMYSNSI"
BASE COUNT         7736 a         3219 c         4559 g         6012 t
ORIGIN      
        1 atacatcaac agctggttat gcgcttgcgg aggtacaaaa tgaatcatga gaagtttatc
       61 gaaaaatgca aggctattgt acgtgaaaga attgaaaatg agattgctga cctaagtgga
      121 gcagtgcctg agtttagtat tttcatagtc tggtcatgca aaacactgca aaatagcaaa
      181 gcattagtca gcgctagctt aaaaggagca ccgtattttg aaattacgtt gaacggggac
      241 aaaggtgaaa tctatgtaga tacttatctc aaaaaatcaa atgaatgtat caaagtctag
      301 caggtgctag gctttttctt tacataaagg aggctgcata atgagaaact actggtatgt
      361 atcactaact aatgaatatc ctcgaaccat tggtgattgt tcagtgcgtg ttgtgcgttc
      421 tgtacaaatc aaagggaagt actctattgt cgaaatgcta agagaagcta caccaaacga
      481 agtggataaa tgcaagctga tatattgcgg tcatggctat tggaaagacg agtatatcca
      541 atacaacatt gagaggtaca tcaaatgaat gataactatg attacatcaa gttgttagca
      601 agtaattatt agatgttttt aggaggaaaa ctatgaaatt aaaagattat atcagagaag
      661 ggtataacgt tgtaactgca cccaatctag cttacaaaat tcaagaagat tatcccaatg
      721 ctttagtgtt tactgataga gctttagatg ccattcctat agggaagtta ttagtagatc
      781 gtttttatag caataatcca gctgttctta gtgctaagcc acttcaaaac gcttatgcta
      841 ttgaacgctt tacttgcgag tttactggat atgagacggt agtaccaatg acaggtgaga
      901 caggaagaaa agtaattcaa cagatcaaaa agaggattgc tgaattgacg ttagatgatg
      961 gaagtaatac gaaccttcta gaaatcaaac taagagatac tgactcagta ccagaagttt
     1021 attataaagg tcaacgaatg gatgagatgc cgataggtct tgtcgatatt acttatcatt
     1081 gggatacgga agaatatgaa tgccaagtac caaaaggagc taacgacatc actattgaat
     1141 attatgattc acataataac aagtacttag atagaaaaat cattgggcac aagagagata
     1201 tgtaaatgat tgatgtttca agtaaacaag caagagctag gttctatgga tcaactgagt
     1261 ggaggaaact tagaagtcaa tgcttagagc gagatcatta tgagtgccaa tggtgtaaag
     1321 cagagggtag actaacaact caacatgatt caattcttga agtggatcat ataaaggagc
     1381 tggagtatca tccggattta gctctagata taaacaacct aagaacctta tgcaaagatt
     1441 gccataacaa aaggcacaac cgtatgaaat atcgtggcca acaaaagaaa agaaagtggg
     1501 atgatgaatg gtggtaagaa aaacagcaaa cagagatgat gtcaaacgag cagaagcaat
     1561 cgctatggtt gataaggcta ttgataagtt ggttctattt aagagtcagc tcattgaagg
     1621 aacaacaact gtagaggata tatctatcaa caatagacta ataggtaacg ctaatgaagt
     1681 aacagggaca tttaatatta atgtagacat tgattatacc ttgagtccat ttgcacatat
     1741 tgtagaaagc aacgagcaag acggctgata aatcagtata aagcgaagat atatagagtg
     1801 aaataccccc ggtcaaaaga ttttgagaag aaataaaatt tttgggaacc ggtggatggg
     1861 gtcaactctg caaataaatg atgctatttt cacacgaccc cctccccacg tagaaaggaa
     1921 gtgagttgat ggctgatctg caagagagaa ataggcttgt ttctgctgaa gaaaggcgtc
     1981 taaataaatt gtttaaggat attgataagg ataaaaagaa ggtcgtttct ggtttgatta
     2041 cgcaagcggc acgcttaaaa attcttcttg atgaaatgtg gattgatatt tccgaaaaag
     2101 gtgattatga gcttttctct cagtcagaaa atcaaaaacc ttacgagcgg gaacgaccag
     2161 ttgctaaaca gtataattcg agagaccaag catatcaacg tgtaattaag caacttactg
     2221 actatttgcc agaagaaaag cgcgaaccta caaaaaatgc agcgctagat ggtagtgatt
     2281 tgctgtgaca acattaaagc cattctattt tgatgaatac gtagatttat acgaaaaagg
     2341 ggtaatccct tttaataaag aaagaattca gttaattact tatttaaaaa acgaagtgct
     2401 agttcgggat gatatctatt ttgatgaaga aatgattcaa aagtttatca agtacactga
     2461 aaaaaatttt tttcctttag ctaaatatca aaaatttatc acacctttca ttttctgcta
     2521 tcaaaaagag gacgacgagg tgttttttga tgaaattcta aatagtattg cgcgaggcgg
     2581 cgggaaaaat gggttcatgt cagcaagaga ctcatttttt attagccctc tctacggtgt
     2641 cagaaattat gatgtaacga ttacagcgaa ttctgagaag cagggaaagg tatcttttaa
     2701 agaagtttat gaaatggtac aaatgcgcaa actggaaaaa caattttatt tgactaagat
     2761 ggctatagtc aacaatgtaa ctaattcgat ttttagttat aggacaaaca atcctaagac
     2821 tatggatagt gcaagagatg gctgcttaga atttgatgaa atccatcagt ttgaatcttc
     2881 agacctagtt gatattcagc gtagtgggct agggaaaata aaacatcctc gaacttttta
     2941 taatggtaca aatggtcatg ttagagaagg tttttatgac aaaatgttgg aacgagcaca
     3001 aaagattttt agtggagaga acaagaatga tcgcttgttt ccctttattt gtaaactgga
     3061 taacattgat gaaatggata atcctaaaat gtggtcaaag gctaatccaa tgttcgaaga
     3121 agattcagca tattcaaaac gtctgttttc tacagtgatg aaagaatata aaaaactaga
     3181 agatgagcca tcaggtaggc gtgaatttgt tgttaaacga atgaatttta cagaaggtga
     3241 tgcagaatca gatattacga ctcatgaaaa attaatggct actaaacaag aattgccaaa
     3301 tttgaatggt cgttcttgtg tggctggatt tgattatgca agtattcgtg attttgcaac
     3361 agtgggtttg ctttttaaaa ttgatgataa atttatctgg cagcaacata gttttgctag
     3421 gaaaaagttt ctagatacat tcaaactgaa agcgcctatt aaggattggg agaaaaaagg
     3481 tttagtgaca atcgttgatg agccatcaat tgatcctcgt catttaattg attggttaaa
     3541 tgaaaaaaga aaaatccatc aaatagaaat cgtttgtgcc gacggatttc gtatggattt
     3601 gctgaaacct cttctagaga aagaatcata taattttgag tttatcagaa atataagagg
     3661 tgtgcagtca aaagttgctc ctattattga agatggtttt gcaaatgaac ggtttatttt
     3721 tggtgatgat cctatgatgc gttggtatac aaataattcg tttatcaaag aagacaaatt
     3781 agggaataaa acatttttga aaaaagagcc agtaaggcgt aaaacagatg ggtttcatgc
     3841 ttttttggca gctttatata aacgagaaga aataactgat atggattatg aggaagcatt
     3901 tgatatgttg gatgagattg aattttaagg tatactcctt attgaaaggg gtagttatag
     3961 aagtgaaaaa gagatatttt ctatttgtta caatatttat aataatagca atttgtgttt
     4021 tgacagtatt tttaccacaa attactgagt atttaaatta tcggatattt accacaaaag
     4081 tagatgatag cgttactagt ttaagtaggc tagaatttat aaaaatatgt gcatctgtgt
     4141 ttggtcctat tttgactgta atggtatttg tgaatacttt aaatatgcag aaaaaaacag
     4201 agcaaaagca aaatgagctt gaagtaaagc aagctaaggc tgagaacgag agagcaaaaa
     4261 aagagctgtt aacacagata gatcgtgagt tctatttttt attaaatatg tttattgaaa
     4321 tacaaaaaga tcaaaatgcc aaacaagctg ttattgcttt acagaagttg gcaattttta
     4381 acgaaaaggg ttttaatcaa gactatgcat ttacttctaa agatttatca gatagtgaaa
     4441 ttgggagtac aattgaacaa tcaggaccgc tgaagggcac agttgaatat ataaaatttg
     4501 aaacatttga tgtaagcaag tcgatgggga attatcaaac aaaaacaaaa gacgttgcta
     4561 caagtttaca cgatcagaaa gtagctatat tgaatgaaca atatgaaaag atgcaaatat
     4621 atcttggacg gtacttcaaa atgtttcacc gaatagttaa aacattaaat gagtattatg
     4681 acgattataa tgattttgat gtgaagaggt atacaaaata tatagggaca ttaagaacac
     4741 aaatttctcc agctgaattc caagtgattt tattcaattc tctatatata aaacgaggtt
     4801 ttggcttagg aattcaactt attggaagtg gtttttttgg agatgatttt gattttgaaa
     4861 ctaatcaaca ttttgaaaca agtatcaatg aacagtggtt tctatcacta tcaactgttg
     4921 acagtgataa ctctataaag cgacaaaaat tatctgaata tattaaagaa ttaggatccg
     4981 tagaatataa aaaaatagct aattttgaaa gtttatataa actctttaat gaaactaagc
     5041 tctagaaata gggctttttt atttttcttt gaaaggtggt gaaaacatgt gagcttattt
     5101 gatatcttta agcagtcaat aagaaatgaa gagccttctg agtggatacc ggattttgta
     5161 tatggagagg aagtatctgc tcgggcatac ctcaaaatta tggcaaaaaa tactgtttta
     5221 gattttgttg caagaactat gtcaacgtta gaagttaagt ttaaaaataa agatgggaca
     5281 tcagactggg attatattct aaatgttcga cctaattctg atatgtcagc agctacattt
     5341 tggcaaaaat ttttttatcg gctcttagat gacaacgaag tgttagtaat atttacagat
     5401 gataatcaat tgctgattgc tgatgatttt agtcgaacgg agtatgccgt gtatgatgat
     5461 gtttttacaa atgtgatggt aaaaaatttt gtctttgaac gttcatggaa catgtctgac
     5521 gtcatttata tggaatataa taacgaagaa ttagaaaggt ttacacgagg tttatttgaa
     5581 gattacgccg cgttgttcgg acggattcta gaagttgcaa tgcgtaacaa ccaaattcgt
     5641 gggtctgtat ccattgattc aacgggaacg gcgaatgatg aaaaaggaaa agatggtaag
     5701 actagatctc aaagattaca agaatatatt gataggattt ggaactcttt taaaactaac
     5761 tcagttgcaa ttgttcccaa agtgaaaggc tttgagtatg aggaatacac caataaacaa
     5821 ggcgtatcga atcaatcgct agatgaactg aataagatga agtcgtcttt aattgatgat
     5881 gttgccaacg ccataggagt acctacggcg cttatttatg gtgaaaaagc tgaactggat
     5941 tcaaatatta aagcttttcg taaattgtgt attgctccac tagttaaaaa acttcaagac
     6001 gaattggtgg caaaaataat tagtcgtcaa gagtacaaaa acggggaacg tatagaagtt
     6061 aaaaaagttc tccaaaaaga tctattagag tatgctactc aaattgataa aataacatct
     6121 ggtggtgcgt tttatattga cgaagttcgt gatgaagttg attatgatga gttgccaaat
     6181 aatgaaggaa aaattcgtat tcgtacgaaa aactatgaag aaactgtgaa gggaggtgag
     6241 aacgacaatg ccgaaagcta aaaaagtacc atttcagttt actaacgaaa ttcagaatgg
     6301 taagcacatt ctcaccttga gtggaaatgt acagaaaaaa tattggcgtg atgatgatgt
     6361 cataaacgct aaagatattc gtgactctct agatactgta accgatgata ttgtaatcaa
     6421 gttgaacagc ccaggcggtg atgtgtttga agggattgag atttacaatt atttaaaaga
     6481 tcacccatca aatattactg tagaagttac agggcttgcc gcaagcgcag caacgtttat
     6541 tgtcgctggt gccaatgatg tgattatgaa tgttgggact tctttgatga ttcatgaagc
     6601 ttctacattt gcttggggga ataaacagga catccaaaaa acgttaaacg cattggaaac
     6661 gatcgatgat tctatcttag ctatttattc agataagact ggccaatctg ttgataagtt
     6721 gcgtgaatgg atgaatgaag aaaaatggtt tacagcagat gaagctgtca aatatggatt
     6781 tgccaattct gtgaagcgtg aagaaccaca aaatgaacct caaaatattg cagcgatgat
     6841 tcaagatgct gttgccgttg caatagcgaa tttaaaggaa cctgttacta ataaaatcga
     6901 acaagaatca aaacaaaaat cattaatcgc acgattgcga aaaggagaat aaagatatgt
     6961 taaaaattac agataaaact gcagatgcaa aaaaggcatt taatgctatt tcttcgaaag
     7021 aagatgcaac acccgaacaa attaatgctg ctctagaaga atatgtaacc gccattgcag
     7081 aagatgccgg caaacaggtg cgtgctgaat atgaagaatt gaaaaatgtg acggacaacc
     7141 gtgtattaga ggctcgtgga attcctacac taacagcaga agaaacaaag ttttataatg
     7201 aagccgtaaa atctggaggt ttcgattctg acgtagtttg gccagaaacg attttggaac
     7261 gtgtttttga aaatcttcaa aacgatcatc ctattttagg cctcattaac tttacaccaa
     7321 ctgtaggtcg tgtcaaagta attagagcac gtcgtaaagg tgttgctgtt tttggaccat
     7381 tgcataaaga ccttgaaggc caactagatg ctgaatttgg tgcaactgaa tttgttcaac
     7441 ttgcgttgac cgcatttttc ttaatttcta atgatacctt agatttagga ccacgatgga
     7501 ttgaccgttt tatcaatctt tctttaagtg aagcagtacg tgatgtttgg gctaaaaaaa
     7561 ttattacagg ttctggtaaa gatgagccaa ttggattgct taaagatcta gatggtgcgg
     7621 tcacaagtgg tgtatatcca gataaagcta gcgcagggac attaactttt gcgaaagaga
     7681 agattgttct tgaacttgca ggggtgatga aaaaattatc taaatacaca tacaaagtag
     7741 atggtgacga tgaaggtgaa gtgaaatacc gttctgttgc tggacgagtg tatctaattg
     7801 ttaatcctgt tgattactgg gatatcgtag ctagagttac atttgcaaat ttaaacaatg
     7861 tgtatgggac tacgatgcca tttatcgctg ttgatcatat catcgaatct gtagacgtac
     7921 cagaaagtaa atgtattgca tacgttgata aagagtatga agctactcaa tcaagagctg
     7981 aaaaagtcta tgtatataaa gaaacttttg caatgaaacg cgcaactttg tatgctgtcg
     8041 acatgcttgg taatggctat ccaacaaata atgatgcagc acaagtatat gacttggact
     8101 ttacagcggg ggagtaatgt cccctgcaat tggtgagata acaccgacga ctgatggggc
     8161 agtcattaat ctttcgtaaa tggggttaga gtatgaacac tgaagaattt ataaaagaat
     8221 ataaggcccg atttcgtatt ttccatactt ccgaagatga ggatatcggg aaacagttag
     8281 agagcgggtt tgccgatata aaatctctaa tcggcagttt tgatccttcc acttatgaga
     8341 aaggaaaaga attagtttat gaacgtactc ggtatctacg gaacgaatca ctagagtact
     8401 tttacgacaa ttttcaaaca atgattatgg atgcatcaat tgatctggca ggtgctggat
     8461 atgccaatta aatcaaatta taagaagcct aaaattgtag ctggtgatct aaatacgcca
     8521 gttacttttt ttgagtttca acctaataag ggtccagaac ctggtgatga agaaaaaaag
     8581 gaactgtatt tttgtacagc actagtttac aacccatcaa tgaaagatcg tgacattctt
     8641 ggcacaaaag aaggcgttac gataaaaata cgggatcccc atcaatctta tattcctact
     8701 aacaaacata aagtaaggat attagattat cgatatggag ataaagtttg ggaaataatc
     8761 gatgtctcac cggatttgga agaaaattca attgtaaaga tagtattaga attaattact
     8821 taaatttggt gataaatgat gagtaacgta aagtttgaag gcgttcaaga aacaatccaa
     8881 gctttagaaa aaaagtatgg agaaaagaaa actaagacat taacaaaaaa agcaatcaat
     8941 gttggcgctg aaaaagtaga aaaacagttg caaacggaca tgaccgtttt taaagacaaa
     9001 ggttatacga tcgctgaggt tgttcgcaag aacgctactt atagagatta caaggcagaa
     9061 gctgaaatcg gttggaatgg tccacatcaa cgatatagaa tcattcattt gaatgaatgg
     9121 ggatatacac gaaagggcaa acaaattaga ccgagaggat ttggtgtaat aacaaaatct
     9181 cttaagaatt ccgagctgtt gtactttgat gctgtaggag aggaggttaa aaagagttta
     9241 tgaaagatat gcttaatatc atttacgagg ctctttgttt gaatgagtat attcattcaa
     9301 tgacctacaa cgagaatacc gagcaatatc gtattaaatt ttatgagcaa cctgaaaccg
     9361 ccgataaaac aggtccaatg atcacaattc gtcctgtaga tacacctaac gaagcgtacc
     9421 acggaagtaa caaagagctt tctgtggagc atatgataca gattgacgtt gaatcgaaat
     9481 atagagcaac atgcaagcag attcaatatg agataaaaaa agagatgaaa aaactaggct
     9541 ttggccaaat ttccggtcaa gggttagatg aatactttac tgaaacaaac cgctttgtag
     9601 acgcgcgtcg ttacgaagga aatacaaaaa tttacgatac cgaatattag aaattaagac
     9661 acgaaaataa cgtgtctttt tttattaccc aaaattagga ggaaaatttt atgactttag
     9721 taggattcaa aaaaatgaca atcggggtct ttgacagcac agggaagatt ccagcagcaa
     9781 atttatatgt aattgaaggt gaacaaaaca aaggggcgac tgtatctgct gaaatcagtg
     9841 ggctatcaaa agaaccatca aaagtttacg gatcgaacat cgcttactat gtctctcaaa
     9901 aaggaacagg cgatgtttca gctacttttg gattgttaga tttaccaaca gaagtaaatg
     9961 acaaaattct tggttacaaa accgatacaa acaaaatcag cttcttggga gaagatactg
    10021 agccaccata ttgtgcaatt ttgatggaat cggaggattt gaacggcgat acagcaatgc
    10081 ttgcgatgtt caaaggaaaa ttcagtcgtg aatctattaa tcttaacact acgacaaatg
    10141 aagctttcga accagaagca gaagaatatg tattttcagc gattgccaat gatgttgaag
    10201 gtgatgctaa aggccaaaca gtcgcaaaat atgttggaga cgaagaagct tctattactg
    10261 gtttgaaaga gatggtattt cctgcagcgg gggagtaact agccccgttg ttggagcaat
    10321 cactcccact attaatgggg caacagttga attaagttag gaggataact atgccagata
    10381 catttaaaat ctataaaaaa aatggtactg aatttacaaa agtcgcagag ggtgaaagtc
    10441 cactttctat tactggaatt gcagcaaata ctcaagttgc taaaggagat tatcaagcta
    10501 cgcgtgtggt cggaggtcaa gaatcagtaa aagtagatat tcctgcattc accactcttc
    10561 caatttcagt tactggtgtg acgttggata aaacgacagc tgaagttgaa caaggcggaa
    10621 ccttaaaatt aactcctact gtaacaccag caaatgcaac agacaaaaca ggaagctgga
    10681 gcagttcgaa tacagctgta gctacggtta ctggaggcaa cgttacagta aaaacagacg
    10741 ctgaagttgg cggaactaca gaagtttcat ttactactac ggatggcggt aaagtagcaa
    10801 aatgtactat tacagttatt gaaaaagcag cgggctagtc ttcggactag cttttttatt
    10861 tggaaaataa tggaggaatc taaatggcaa aagtaagaat tgaattgaaa gaagaatcag
    10921 gaaagaaagt ctatgaaaac atagacacaa cagggaaaga ttatcgaaaa gctttagaaa
    10981 caatcaaaaa gctaaatgaa gacggagtaa tgatttggaa tcgtttagac atttatttgg
    11041 attttgctgt aggtatcttc agggatagca aattgacagc tgaacaaatt cttgatggct
    11101 taccatctga gaaggtaatg agcacattgg acgacatcct aggagaagta atggggattg
    11161 aaagtaatcc agacccagac gcaaaaaagt aacacccgaa gaagcagaag aaatgtactt
    11221 aaacctatgc agagagttag tgaaagcggg atggtcacta tctgacatag aagataattc
    11281 ctttgaaatg atgatgaaaa ttgcgtgcac ttcgcctaag aaagagaaac aaaaagaagt
    11341 tgatcttaaa gacttcttga aatccatgta gagaggaggt aaacatatgg caaatggaaa
    11401 acctttaggc aatatgatta tcaaactaga cttagacagt tcagcttttt caaaaggtct
    11461 agccggagct aaaaacgctg ttaaccatca aatgaaagca atgaaatcac agatgcaagt
    11521 aatgaactct tcaggaaatt ctctcggtgc tttacaagca aaatacaatg gtttaggcgg
    11581 agttctacaa gcaaatgaaa agcaagtaga attgctcact aaggcttata aggatagttt
    11641 tgactctaac ggaaacgcta ctgcttcgac tgctaaatac gctaatcaat tgaatcaagc
    11701 aacagctaga tcagcaagtt acgaatctca gatgaaaaca actgttggac agattgctcg
    11761 gatgaaagtg gaaacggaag ggtttacggg gaaactaaag gctaattctg atgtattgat
    11821 taattctggg aaaaaaattg aagcctttgg taaaggagtt tctgggatag gatctgccct
    11881 aactgttgga gtaacagctc caatagtggc aggcgcaact gctgttacta gtgcagctat
    11941 ttcatgggaa agtgcttttg caggtgtaaa aaagacaaat gatgaagttg tagactcgaa
    12001 tggaaacgta gtttattcct atagcgactt agagaaaggt ctgcgtaatc ttgccaccca
    12061 attacctggt agccatcaag aaatagcaaa tgtagccgaa gcagccgggc aattaggtat
    12121 aaagactgaa aacgtagtta gcttcactaa aacgatgata gacatgggtg aatcgactaa
    12181 tatgtcggct gaaactgcgg caacttcttt agcacgattt gctaatatta cccaaatgtc
    12241 tcaaaaagac tttgataaat taggatcagt cattgttgat ctaggtaaca attttgctac
    12301 aaccgaatca gaaatcactg aaatgggatt gcggttagcg ggagctggta aacagattgg
    12361 aatgagccaa ggcgaaattc taggatttgc tactgctcta tcttctgttg gtgtcgaagc
    12421 tgaagcgggt gggtctgcct tttcgaaggt catgatccag atgcaactag ctgtagaaaa
    12481 aggatttggc gcttttgatc agctgaaaca gatggcagaa gaacaaggtg ttccgtgggt
    12541 taatttagta aatgctgtga gagacggtgg aaaatcgtta aaagcagtat cagagcaaat
    12601 gggattcact tcatctgatt taaagaaaat gtataaagaa gcggacaatt cgaaaagttc
    12661 gcttgaaaat tttgcgaatg tggcaggtgt tacaagtgaa caattccaga agttgttcaa
    12721 gagtgatcct tcagaagcaa tcatcaagtt cattcaaggg ctgaaagatt cagaaaaaca
    12781 tggtacttca gcaatcaaag ttctggatga catggatata aaagaagttc gactacgcga
    12841 cagtttattg agagctgcaa atgctagtga catattcaaa ggagcaattg aaaaaggaaa
    12901 cggcgcgtgg aaacaaaata cagctttaac aaatgaagct aataaaagat acgaaacaac
    12961 tgaatcaaag ctaaagatat tgaaaaacga agtgattgat acagcaatcg atcttggagg
    13021 tccatttgta gacgcgttgc gtgatggtct aaatgtatcg aaaccattga taaaaaactt
    13081 agggaatatg gcgaaagctt tctcagctat gagtgatgat caacaaagaa acattatcaa
    13141 atgggttgga ttagcagcag cagctggtcc agcgctgaag cttttaggaa ctggtacttc
    13201 agtaataggt aaagttactt caactactgg aagattgacg aaaagccttg ttgatttagc
    13261 agcgaaagca gcagaaaaga aggctatgga tgcctttgca tcgtcggtaa caacagctgg
    13321 tgctgcagct gcaaagacag caggagctgg aggtattggc gggttagtcc ctatgatggg
    13381 aaaaacagct agtgctgcaa gtactgctgc gggcgccagc ggaataggcg caatgacaac
    13441 gtcactaggt ttgttaggtc ctgctctact tggtattgtt ggcgttggtg gcgcactggc
    13501 agttggctat ggagcatgga aattatttgg tgaagaagct tggaattcct cgcaaagagt
    13561 aaagcaatgg ggaacagatg tcggtgagga gatagatagt actcttgacg gagtacagaa
    13621 gaatattgaa ggtgcaaatg gtcaattcgg tcttctcaaa gagggtttta ctgttgatga
    13681 tgcaaataat atggcggcta atttcgcaac cgctggaaca tctttggaaa catctttaac
    13741 gggtagaata aaggcattag acgatgcact aaaagggcta cctcaatcag ttcaggatgc
    13801 catgaaagaa gtagctgaat cagaagaaga aaccttaggg caatctcttt ctaaaataaa
    13861 agacaacaat gaagaaataa aaaaaatcag agaaagagcc gcaaatcaga acagagactt
    13921 gactgtttca gaactacaac agatacagga tctaatgaaa ggaacttctc aagcgtatgt
    13981 ggatacgctc gatgtttctg ctaaagacag aaaaaagata ctatcagcca tgaatggtga
    14041 tgttgcacaa gctacagaag aagaagcgaa gacgtggtta caatctttag cgaaacaaaa
    14101 agctgcaact accgaaaatt atagacaaat gcttgaagaa aaaaaggcgg ctttaaagga
    14161 aaagggagta agcaaagaat tcattgatgc attagaagat gaaatgaatg attacatcaa
    14221 aactacaaat caaggattcg atagccaaat agctgctata acaaataaat atccacaatt
    14281 gattaacgaa gtgagtttat caaatggtca attatttagc aaaacggcag ctgctatgga
    14341 caatactggt cagtatacaa aagtttttct cgaaagtaat gaaaagattt tagcccaagc
    14401 gaagaaaaca tcggatgaaa tagcagataa tgccgaaaaa aatgcagaac aacttggtat
    14461 ggtagctgat gaggctacgc atgctggatc aacttggaat ggcttagttt tagacgaaaa
    14521 aacaggaaaa gtaaaaacta atgtacaaga agtagtgaat gaagcaacga aggatttcac
    14581 taaatggaat gaattgaaac ctgttgttca tgatgcgaaa ctaacaacga atgcaaaaga
    14641 tgtcatttcc atagcggcag tacaaaatgg ctattgggat gatatggaat ggaaagaaaa
    14701 agaagcatta ttagaagatg agtgttctaa aaatgtatac aaggctttag aaaatgctgg
    14761 taaatggaat gagttagata ttccttctaa agaagcaatc ctaacttcaa atactcctga
    14821 agttatgggt gaaactctca tgaatttggg attatgggat acttacaaac ttcaagtgaa
    14881 agatttagat ttgaatgcgt ataaagtata cgatgctatc gatggatcag aagaaaaatt
    14941 aaatagttgg gctcaattag ataaccccac aaaagaattg ttattagata actcagattt
    15001 ttctacaaaa ctttttaatt ccgaagaaat gtttaaacgt tggaatcagt tatcatctaa
    15061 tgcaaagttt ctttcggcta ataatactaa atttacagag gttcttttat catctgacag
    15121 aatgtggaat caatgggtaa accttcctga taatgagaaa agattacttg cagataacga
    15181 agatttagct accactgttt tatcttcaga agaaatatac ggaagatgga ctcaattacc
    15241 agataatcaa aaatatatgt tagctgacaa tactgactta gcaaacaaaa tattcgcttc
    15301 agaagaatca tggaatgcat ggaaagcaat tccagacaca gttaaaaact tgcttggtaa
    15361 taatattgat ttactggcta aagtgaaaga cgggactgta agtgtagaag aatataacaa
    15421 agttatgcca cttcttaaaa ctctatatgg agataacgtt aatttaaaag aggcaatcag
    15481 tggggctaaa agctctatag agaattacaa caaaaatatt tttcctagtg ataaaactgc
    15541 agtaggacac gatgaggctt cacaagctgc taaagatgca tttgaagcgt tcaacatctt
    15601 ccaaacaaaa ataccagata aaattactaa aactgtatca gctgacttta taggtcctat
    15661 gccgaaaaat gcgaaaggaa caaacttcca tcctggcgga gcagctatgg taaacgatca
    15721 aaaagggcct acttatgaag agcttataac cttgccaagc ggagaaactt ttatacctaa
    15781 aggacgtaat gtcgttttag acttaccaag aggttcgaaa gtactgaacg ccactaaaac
    15841 taaacgtcta gtgcctaaat atgctgatgg tataggaaac ataacgacgg tttcatctac
    15901 gctaaactta gatgctttaa ttttagctat taacgagcta acgacggtat tgagaactca
    15961 actgatggca ggaacgactg atgctaaaga tactaaacta tcagatgaaa caaaagttgc
    16021 cgatccgatt attccggaca gtctttctga aaaatcagat cagtatctag gaattggagc
    16081 tcaatggtta actaatctaa tgaatggttg gaactcagcg gttcctcaat atatgaatag
    16141 tgaaatggtt tttattacaa actatctcaa tgcgctaaaa cttcaaaaca atcctaatta
    16201 tacgcaagga gcaacatgga ataagaactt actgaatgga tggaatagtt taactgggac
    16261 ctttattgct acaatcaatt cattttgtaa tcaagcaatg gtcacgctta gaaactacaa
    16321 cactcctatg tacaacaatg gccgaacgtg gcagcaaaat aatcttaatg gttggaattc
    16381 attgtatgga tcattcatag cccgtgtaaa tcagcttggt aacgattcga ttaacaacct
    16441 tcgttctaaa agtggtggtt tttacaatgc agggacattc ttgttgcaat ctttaataaa
    16501 cggaatgaat tcgttaggta attctctttc tactacaatg aacaatgtcg ctaatacaat
    16561 ggttggtggt ataggcaaag gagttaatgg cgtcatatcg ggagttaact atgtgcttaa
    16621 ggaagtcgaa tcaagtaaga atatcggaaa ctggccaatt ccacaatacg ctaagggaac
    16681 tgatggacat cctggtggac ttgctatgat taatgatcaa aaaggcctag ttcacgaaga
    16741 atatgttcag atgccagatg gacgtagttt tattgccaaa ggtagagatc ttcttgttaa
    16801 tttgcctaaa ggtgctcagg tcttaaatgc ttctttaaca aagaaattaa aggaacgttt
    16861 aaatattcca cactacgaaa aaggtattgg caatttagat attgttgatt tgttagatga
    16921 tgaaaaaaga atgttagaat tcttaactag caaagttgat ttttcaggca ttaatgaacc
    16981 atggcttgat atgactaaat caggaacgtc attaatgtcc aaagctgcaa atacaatgct
    17041 tcaatcaaaa ttaagtgaat tcttcactca tggcaatttt gatggtgcag taaatgccaa
    17101 tggtgtttat caatatttag ttgatgtggc acagaaagta atgggtaagt tccccggact
    17161 tacagtaact tctggatatc gagcaggaga tgcctactat cacggaaaac ggcaagctat
    17221 cgacttggct tatccaggta tttctggaga tccgagatat acagcagcag caaattacgc
    17281 ttttgaaaaa ttcccttcaa aaattgcgta tgtcattacg aatggacgtg tacgtgaccg
    17341 catgggatta tctggaacaa ggtcaagcgg acaatggacg aattggccag atggcgatca
    17401 cttcgatcat attcatttaa acggttcgat ggggtctggc gatatattca atggaggagt
    17461 tggaggaagt ggtgttgcac gctggcgttc ttatgtgtct aaagcattga aaatgaatgg
    17521 attaccagct acagcagcgt atataaatgc ctggatgtcc caaatccaaa cggaatctgg
    17581 tggtaatgag aaagcaatcg gcggcaatga tgggctcgcc gaaggcaatg caacaggatt
    17641 acttcaaacg aaacccggaa cttttgcagc taacgctttt cctggtcacg gaaatattat
    17701 gaacggattt gataacatgt tagcagcaat caattatgct aagaaacgtt acggagttgc
    17761 aggtatgttg caagttatcg ggaaaggaca tggatatgcc aatggcggct tgattaccaa
    17821 agacggacta tatcgagcag gagaaggaaa taaaccagaa atggttattc cattgacaag
    17881 aaaaacaaga gctatcgaac ttatgggtca agcattagcc ttcctttctg gaagcaataa
    17941 aaaagaaacg aaaagtgaga ttaattctgt agataataga catatcctac aagcgataga
    18001 aaaacagtct cgtatgacaa atgatctgct atcattatta ctctcgtttt ttaaaagtaa
    18061 caatcgatca gataaagaac tagctttaga tattcagaaa attttaatca ggaggacata
    18121 gtatttgagg acagtaatat taagaaataa attgaatgaa gaaattgatt tgtccacaga
    18181 agattatttt gctactgaaa tgtcgaatct aggttttgaa gttacaaaag agcatattgg
    18241 acaatggggg agtttcagag aaataggaga gagtattgaa ttgtctgaat ttcaatcatc
    18301 agttatgatt tctgttcacg gatttagaga acaacaatta tatggttcat tagttgaatt
    18361 tctggctaat ggaccttttg aactagaatt ttcttttgat tcagaaacaa tgatcaggaa
    18421 atgtagtttg aaatcattat ctaaaacaga aattgattcc caaagctcat tgctaacaga
    18481 tactttgaat ctatatttta cttcaagctg gtattctatt aagcgtgaaa aattaataca
    18541 gaattcaact tctctcaata cacgtggaaa agtttactca tttgttcgac catatgtcta
    18601 caccaaaaat gtatgggaaa agaaaggtgt atttaagttc agtaataatt ctgtatattt
    18661 gacaaatagt acagagcgaa tgtctccgtt aaaaataagg attattggag agtgctcaaa
    18721 tccatattgg gaagttatcc aaaactctaa agtagtagct tcagatggct attttattaa
    18781 tatgaaagac acacaaatat tagaagtgtc tagtttgcta gaagaccaaa cggcaatttt
    18841 aaaagatgta ggtggagttg aatcttctgt ttatcaacaa caagatttta ctaaatcaaa
    18901 cttcgtccaa gcgcctctag gagaattcag cgttgttttt catgttggac atgcaaatgt
    18961 agagattgaa ctttatgagg agcgtgatct attttgattt tagccattac cctctttcac
    19021 cgagatatga atttatataa cgaacgttta atttcgactg agttcgaatt tggaacggat
    19081 gaaattggag aagaagtaag taattttatt acagataaat atgtccatgt aaaatcagga
    19141 gattttctac tagcaaaatt tattcctagc gggaagattg cttattttgg agtgattagc
    19201 tcgcaagaag acaaaaaaat aaattgcaaa ggattactca gtttggccga tagcgagata
    19261 ccgactgtaa aaatatcagg tgctaattat gaagagcata ttcgacgact gattgaatac
    19321 tacttattga acgatccaac aaaacaacta aaaaatattt tggaggtaaa aacagaaagc
    19381 gttacttctc attcttatca agctgttgac acaaataaga gacgattgaa tgcatatata
    19441 ataaatgggt tcaaaaaata taatgttaaa tggtatgtca aagagattaa aggtaaaaaa
    19501 atatatactg gtattcgagc agttgatgat tcaatttata ttaaagataa ttcatctgag
    19561 tttaatgact gggatgtttt tgtgcaatct cctggaccag ggaacgagaa caaattattg
    19621 atcgtagata aagcaatgaa agatatagaa aatcccttgg tattgtctac gtggtattta
    19681 gatgacgaaa ataatcttac gcaagattca acgaataaga acattgcatt gcctaccgtt
    19741 aatttagtaa atatatatga tcaaactcaa gaagatagac cttcttatga agaggtagct
    19801 aaatcggagc ttaaaggcaa cgcctattca catgaaatta aagttaatgt tgtgctaaat
    19861 gctagaaata tcaatgtaga aaatatcgaa acaggaatgt tagcgactat tgtttacaaa
    19921 gagcagactt acaaatctgt tctaactgcc tggcgaatat ccagcagtaa aaagaatata
    19981 gaattgacat ttggtaatgt ccgaagtcgt tttatggatt attttgaaga taatggggga
    20041 taaaaaatgg ttagcaatgt ggatggatat caatttgaaa acgtgaaagt aagcgcagaa
    20101 aatgatgcta gactttatca cgttttatat aatcggaaaa atcaggttat tgatggttac
    20161 gatcagtcta tgaatttatc ttcaagcgga ttaacagtaa aagttgctgc aggagcagcg
    20221 attattcaag gtcgtatggt cgttgttcga caagaagaaa gtataacagt tccagcaaac
    20281 tcaagtggtt atatagcatt aacagtggat ttgacacaag aagttatacc tggatctatt
    20341 cttccagaat cggaagaata tgaatggact aataatcaag tcaagctaga atttataaca
    20401 aaagttataa aaggtaattt aaacaatggt gataaggtct ataatttacc actatgctca
    20461 gttaattcta ctggatcaac tgtttcaatc tcaaagatat cggatagtta cgagctgact
    20521 ctctctaaag gagaaatttt gtggaggggg actgcgttaa tgcatgatac tcaaactgtc
    20581 caaccttcaa aaaaaatttg gcagacagtt tcaggttttt tgttagtatg gctcccatac
    20641 gaaaacgggc aagcaattga ggatagatat gtaactacgc ctttttataa ggagcgtata
    20701 acttttacta atgttttagg agaaattgtt tcaggatttg atgactatca caaaaagtgg
    20761 tttagtaaac gaataaacta taattcgaat actaatatat ttacaggtgc agcaagcaat
    20821 gcaagtggag ataacgcaaa tatggtgctt aggtatatag tttcttttta gttaggaggt
    20881 atggaagagt ggcaaattta gaaattaaat tatctgcaaa taagagacag ccttatctac
    20941 gtcaccgtgt tgttggtaga gttggtgatg gggggctcac aacaatcaat gtacaacttc
    21001 ttgaggaaga tgaaattaca ccttttgtaa ttaatctaaa cggtactttg aaatttgtgg
    21061 gcgaagtttc aaacggtaac tataccgagg gagaaccaga aataatcgat tcgactaatg
    21121 ggttaattag ttacacgttc actaagtcaa atttcagcac gagtcatcaa ttcaaacaag
    21181 catattttga atatgtagat cctaacggca aaaaagtaac ttttcagaac ttcatcatag
    21241 acgtgttaga acgagtagat attaattcgg agcaagcgaa atactatatt tcttcattgg
    21301 aaaaattaca gagtgaaatg caaaccactt tcaatcagtt cattagtgat aaacaggtcc
    21361 aatacgatca aatctactcg aaatataacg aattagtaag attgataaat gaatcagata
    21421 agcaagtaaa tgatcgtatt gataaggcaa ataatcgtat tgatcaaacc aatcagcaaa
    21481 tcggcgatct cggcaagctg aaaaagatgt acagtaacag cattga
//
LOCUS       DAJSBL010000042        21430 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.042, whole genome shotgun sequence.
ACCESSION   DAJSBL010000042 DAJSBL010000000
VERSION     DAJSBL010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21430
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.042"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            111..812
                     /locus_tag="ONT85_001912"
     CDS             111..812
                     /locus_tag="ONT85_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HCR2378881.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
     gene            complement(1421..2437)
                     /gene="tsaD"
                     /locus_tag="ONT85_001913"
     CDS             complement(1421..2437)
                     /gene="tsaD"
                     /locus_tag="ONT85_001913"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HCR2378882.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIIAGGVAANQ
                     GLREGLQDALAAKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(2434..2898)
                     /gene="rimI"
                     /locus_tag="ONT85_001914"
     CDS             complement(2434..2898)
                     /gene="rimI"
                     /locus_tag="ONT85_001914"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HCR2378883.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLAEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(2905..3447)
                     /gene="rimI"
                     /locus_tag="ONT85_001915"
     CDS             complement(2905..3447)
                     /gene="rimI"
                     /locus_tag="ONT85_001915"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HCR2378884.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTVEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(3431..4153)
                     /gene="tsaB"
                     /locus_tag="ONT85_001916"
     CDS             complement(3431..4153)
                     /gene="tsaB"
                     /locus_tag="ONT85_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HCR2378885.1"
                     /translation="MITLGIDTANQTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAID
                     QLFADLQISPKDIDRIAVSDGPGSYTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAAN
                     CIGVDNIIVPMFDARRKNVYAGAYRWKENRLETVLEDVHISASDLFTQLQKLNEAIYF
                     VGSDCHKFFSEIKEILPSAMINTVPAWDIPSGVTVAQLGALVEPVAEKQAFLPRYLKR
                     VEAEEKWLETHTPGDESYVEKI"
     gene            complement(4344..5324)
                     /locus_tag="ONT85_001917"
     CDS             complement(4344..5324)
                     /locus_tag="ONT85_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR2378886.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPADGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            complement(5348..6832)
                     /gene="galT"
                     /locus_tag="ONT85_001918"
     CDS             complement(5348..6832)
                     /gene="galT"
                     /locus_tag="ONT85_001918"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HCR2378887.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLEDYPQVIAGVVKWPMSVIRLQSTEKNLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            complement(6844..7833)
                     /gene="galE"
                     /locus_tag="ONT85_001919"
     CDS             complement(6844..7833)
                     /gene="galE"
                     /locus_tag="ONT85_001919"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HCR2378888.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREILGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
     gene            8081..8248
                     /locus_tag="ONT85_001920"
     CDS             8081..8248
                     /locus_tag="ONT85_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HCR2378889.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            complement(8310..10121)
                     /locus_tag="ONT85_001921"
     CDS             complement(8310..10121)
                     /locus_tag="ONT85_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HCR2378890.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(10118..10483)
                     /locus_tag="ONT85_001922"
     CDS             complement(10118..10483)
                     /locus_tag="ONT85_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HCR2378891.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            complement(10646..11041)
                     /locus_tag="ONT85_001923"
     CDS             complement(10646..11041)
                     /locus_tag="ONT85_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HCR2378892.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            complement(11059..12021)
                     /locus_tag="ONT85_001924"
     CDS             complement(11059..12021)
                     /locus_tag="ONT85_001924"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HCR2378893.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            complement(12021..12233)
                     /locus_tag="ONT85_001925"
     CDS             complement(12021..12233)
                     /locus_tag="ONT85_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HCR2378894.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            complement(12254..12952)
                     /locus_tag="ONT85_001926"
     CDS             complement(12254..12952)
                     /locus_tag="ONT85_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HCR2378895.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            complement(12972..13514)
                     /locus_tag="ONT85_001927"
     CDS             complement(12972..13514)
                     /locus_tag="ONT85_001927"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HCR2378896.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            complement(13646..14650)
                     /locus_tag="ONT85_001928"
     CDS             complement(13646..14650)
                     /locus_tag="ONT85_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HCR2378897.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILPSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            complement(14647..15636)
                     /locus_tag="ONT85_001929"
     CDS             complement(14647..15636)
                     /locus_tag="ONT85_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HCR2378898.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVEKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            complement(15633..16439)
                     /locus_tag="ONT85_001930"
     CDS             complement(15633..16439)
                     /locus_tag="ONT85_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR2378899.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            16605..17561
                     /locus_tag="ONT85_001931"
     CDS             16605..17561
                     /locus_tag="ONT85_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR2378900.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            complement(17638..18156)
                     /locus_tag="ONT85_001932"
     CDS             complement(17638..18156)
                     /locus_tag="ONT85_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HCR2378901.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(18244..18393)
                     /gene="rpmG"
                     /locus_tag="ONT85_001933"
     CDS             complement(18244..18393)
                     /gene="rpmG"
                     /locus_tag="ONT85_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HCR2378902.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            18621..19067
                     /locus_tag="ONT85_001934"
     CDS             18621..19067
                     /locus_tag="ONT85_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HCR2378903.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            complement(19262..21157)
                     /gene="asnB"
                     /locus_tag="ONT85_001935"
     CDS             complement(19262..21157)
                     /gene="asnB"
                     /locus_tag="ONT85_001935"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HCR2378904.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
BASE COUNT         6445 a         4549 c         3699 g         6737 t
ORIGIN      
        1 atatttgtaa agtattaaaa cttgtttcta atgttggtat gttaaaactt tacaaattct
       61 ttaaaaaaag tgacaataaa tatagtgaac aataaggaga ggacttttta atgcgtgtta
      121 ataaaatcga ttttcacttt tcaaaactca gatatcaacc tgattttgaa catttctcgg
      181 atgaagagtt ccaacagatc aaggaacata ctgttcttcg ttcttataaa aaaggacaga
      241 tccttttcga cgatggtgat cccagacaac ggttgtatat cttgaccaaa ggactcgttc
      301 gtttagaacg ttatgatgaa agtgcaactt attactacta tgactatgtg aatacaaata
      361 acctttttcc tatgtcagga atgtttggag aagagaccta cacttataca gctcaagcga
      421 tgactgatat tgaaacattc taccttcctg tttcctacta tgaaaaatta gcacgaaaaa
      481 atcccgaaca gttgatctat atgatacaaa aaatttctaa agtagttgaa atacatgaat
      541 tgcgtatcca aatcggattg acctctagcg cctttgaccg ggtaaaacaa aatttattca
      601 ttttgaaaga agaattgggc gtaccaacaa aagatggtca tatcaccatt ccttatccga
      661 tcaccttaaa agaattagca gtgaatagcg gcacgacacg tgaaactgct ggacaagtaa
      721 tcaaacaact acgtgattcc gggctgttag attatgacaa aaagcgtttt actttctgta
      781 agacagctac tactccagct atgagagaat aacgcgaaca aaaataagca gtgaccctga
      841 taaatatcag ggtcactgct tattttcctc cctaaaactt ctgaaagatc cggcagtttt
      901 agatcactgg acaagactca aagattttct atgtgtgttt cagttatgta atattcactg
      961 tttgcaaaaa atactgttag attaactaga tgaagcgtct tgtcctcatc cgatctagat
     1021 tattcgatat tcctcgacaa taaacgatta ctcagtgagg gcaatcagag gaacggataa
     1081 tgataaataa gctgtaaaaa atagcaaaat aacaataatg attaaaagta tgtaaaaaat
     1141 agggctttga caaaaacgtg tcacagccct atttcgatta aacggtgtgt acctgtcaac
     1201 tcttcggtca caagtcataa tgttcataaa catgaggagc tgttttttga aattgttcct
     1261 tgtgaatcag agttaaacga caggcttacg aactcttgat taaacggcgt tcaaaagctt
     1321 gcatacgcgg taataagaac agccaggtca aaaacatgag aagccgtttt tcctgggctg
     1381 ttcttattac ttgatgcagg gcagcttttt cacaacctct ttattcagat aaaaccaagc
     1441 ttggatcagc attcaactga tagctagcaa aatttctttt ttgcatttcc acatgtgctg
     1501 ctgctccaat cattgcagca ttatctccac aaagtcgaag agggggaatc actagttcta
     1561 cctcagggag tttagctgcc aaagcatcct gcaatccttc acgcaaccct tgattggccg
     1621 ctacacctcc agctataatc agttgcttga ctggataatt ttgacaagct cgaagtgttt
     1681 tagtaatcaa tacatcgatc acacttgctt gaaagctagc tgctaaatca tttttgtcta
     1741 gatcttctcc acgctgttgt gcattatgca ccaaattgat aaatgcactt tttaatccgc
     1801 taaaactgaa atcataatta tcttcatgga tcatcgctcg cggaaaatgg taattatctt
     1861 ttccctgatg agctagctga tcgatttctt ttccgcttgg gtaagaaaga cctaatactc
     1921 ttccaacttt gtcataagct tctcctgctg cgtcatctct tgtctcccca atgatttcat
     1981 atgatccatc ttcttgcata tagactaatt ctgtatgtcc tccgctaaca agaagtgcca
     2041 tcaatggaaa ctggaatggt ttaaccagac gggctgcata gatatgccct gccatatggt
     2101 ttacaggaat aagcggcaat tggtgtgccc aagcaaaagc cttagctgca gaaataccaa
     2161 tcagcaacga gccgacaagt cctggtccat aagtgacagc gactgccgat aaatcttctg
     2221 ccgacacacc agcttctacc agtgcatctt ccaagcataa ggtgatttgt tctacgtgat
     2281 ggcggctagc tacttcaggt actacgccac caaaacgttt atgactgttt atttgagaag
     2341 caacgatgtt agataagatc tctgttccat ttctaacgac cgctacactc gtttcatcac
     2401 aacttgattc aatcgccaga atcaattctt cattcatttc atcgtctccg tttttaactt
     2461 tctgctcata atgatcgcat cttccgtcgg attgcgataa tatgatttcc gtttacctaa
     2521 tatacgaaac ttttctgatt cataaagttt tcttgctgct tcattagata gacgtacttc
     2581 taaaaaaaca tgcagcgaat cttttatttg ttccgtaatc agtaattgta acaaatgccg
     2641 tgcatgccct tttccttgtt ctgaaactgc aacagcaata tttgtgatct cgacttcatc
     2701 tattactttc gagtatccaa taaagccaca gatctggtta tcttcagcta aaagcagata
     2761 atcagagtga ggctgttgga tgtctgccaa aaactgcatt ttcgtccaag gtgaaccatg
     2821 tggataagca ttttcgctaa tcatccatac gcattctgcc aagtacgctt catcaaaatc
     2881 tttttttctg caaatcaact aatctcactc attttctaaa agttttatca tatccaacgc
     2941 atcttctttt gtttcagtgt aatatctttt tttgatcgtt gttgcttgaa accccaattt
     3001 ccgataaagt ctttgtgcat cttgattact catacgcact tctaatgaca tcgtctcaca
     3061 gcgattcatt atagcaaagt tctcgatttc gtcaataagc agcgaaccga ttcgtttacg
     3121 ctgatatgcc ggtcgaacgg caatattcgt gatatggcaa tctaaaccga acgctcttga
     3181 accgataaaa ccaatcattc ttccattatc aaagatacct agatatagat gaggaagcgg
     3241 tgacttcaat tctgatataa aggcactttt tgtccatggc aattcgccat tatagacttc
     3301 tcgttcgaca tccagcagct ctttgatatc ttctgtagtc aattctctta gtacgtaacg
     3361 acgatcgatt tgttcgactg ttttttctgt ataaatcatt ttatcttgaa aaaagcttct
     3421 ggcatacgct ctaaattttt tcaacataac tttcgtctcc aggtgtgtgc gtctccaacc
     3481 atttttcttc tgcttctact cgttttaaat agcgaggaag aaaagcttgt ttctctgcaa
     3541 ctggttcaac taaagcacct aattgagcca cagttacacc acttggaata tcccatgctg
     3601 gaactgtatt aatcatcgca ctaggcaata tttcctttat ttcagagaag aatttgtgac
     3661 aatcactccc gacgaaataa atagcttcat tcagcttttg tagttgtgtg aacaaatcag
     3721 aagcagaaat atgtacatct tcaagtacag tttctaatct attttctttc cagcggtatg
     3781 ccccagcgta tacatttttt cttcgagcat caaacatggg cacaataata ttatccacac
     3841 cgatgcaatt agccgcgatg gttttcagac tagatactcc tactagctct ttatctaatg
     3901 tataagcaat cgtttttgct gtcgttacac ctatacgaag ccctgtataa gaaccgggac
     3961 catcagaaac cgcgattcgg tcaatgtctt ttggacttat ttgaagatct gcaaacaatt
     4021 ggtcgattgc aggcatcagt gttacactgt ggttgcgttt gatatttgtt tgtatttgtc
     4081 caagaatttg ttcgtcttct accacaccga tcgccaaagt ttggtttgca gtatctattc
     4141 ctaatgtaat catatatttg atcctttctg ctatgacatt ctcgccttcc attgtagcat
     4201 acttttttat tctgaggatg aaaataccaa aggcaaaact agtacaataa tacatgatta
     4261 tctgctggcg ggcaaatttt gccccttcct gaaacacaaa aaagctgcgg tatcactcca
     4321 ttttcccaca gcttttccat cttttatttc gttgattctc gaataatcaa ctctgtttcc
     4381 acaataatct ttcttgaaac cggtgctttg gaattactta attgaagaat cgtctccact
     4441 gctagctctc ccatccattc ggtataaact tttacagtag aaagcgaggg cgttacatat
     4501 ttcgctacac tgacatcatt gaagcccatc acagaaatat cttctggtac cactaaacct
     4561 ttttcttgaa tcgctttcag tgcgccaatc gctaatgcat cattagctgc aaaaagcgca
     4621 tcaggaacat gcggattcgt ttttaaatat tgcttcactg cttgatatcc tgactcgaca
     4681 gaaaaatctg cttcgataat tggaccttcg tcgtagcctt tttctgccaa aacttgttga
     4741 aatgccataa ttctgggatc ttttagccgc tgatggtttt tcttcgtata ttctgttcca
     4801 gcaagcatag cgatatgttg cgctttatgg gagacaaagt gttcaatgac tttttgaaca
     4861 cttgacttaa agtcgacaac tagagagtcg attccttgag aagtgccgtc agaatctacc
     4921 aaaagcaagg cattttggga tcctttcatt tctaataact gagcttcgtc gaattttcct
     4981 agtgcgatcg ttccatctgc cggaagatca ctaatggaat cccacgtttc tttcaataaa
     5041 gtaacgtttg cttcttctgc ctttttttcg attcctaggc gaatagataa ataataaaga
     5101 tcttcaagct cttccgtatc gtcgtaccac tggatcaggc gaagcgtttg cttagtattt
     5161 gctgcatttt tacgatgttt cgtataattt aagcgttctg ctgcttcaaa aattttttgc
     5221 tttgtggcta agccaacgga aagatcagga tcatagttga gaactcttga aacagtagca
     5281 ggagaaactc ctgccaactg cgcaatgtct ttaattgttg ccatttgtct ctcctccttt
     5341 tttacgttta taaaatcgca gtaaatcgtt caaacgctgc ctgtcctttt tcatctcgct
     5401 tgaatactcc agcgtcttcc agtacacgtg caaaaacctg acctacagat ttttgtacga
     5461 catcggtgac cgtatctttc gtcaaatcag gatgttcttt ttttaattgt tctgcccacg
     5521 gacgatgata ttcagccatc tggttttctt ctcctaataa gaaatgctct acttctacta
     5581 gttctggttt caaacgaggt ggtaaaacag caagccccat tacttcgatc aacccaatgt
     5641 tctccttttt gatatgttgg acatctttat gtggatgaaa aatcccatca ggatgttctt
     5701 ctgaaacatt gttgtctcga aggacaaggt ccaactcaaa gtaattgtcc cttcttcgtg
     5761 caattggcgt aatcgtatgg tgtggtgtac catctgctga aaaagctctg atagatactg
     5821 tttcatccga atatgctttc catttttcca atactgagtt agctgcttca ataagcaagt
     5881 ttttctctgt tgattgcaaa cggatcacag acatcggcca tttcacgact cctgcgatga
     5941 cttgcggata atcttctagt ttgataagtt tttctatcgg tgcttttgcc ataggaaatt
     6001 catgacgtcc tgcctgataa tgatcatgag aaagaatcga tcctccaaca attggtaagt
     6061 cagcattcga accaacaaag taatgaggca agacttcagt aatccgaagc agtctagcaa
     6121 atgtttcctt tgtgattttc atcgggcggt gttgttctga taaaatgatc gaatgttcat
     6181 tgtaataagc ataaggggaa tattggaatc cccagctttc accgtctaga ttcattcgaa
     6241 taatccggtg attggtgcgg gctggatagt tcaagcgacc tctatatcct tcgttttcca
     6301 tgcaaagcat gcaggtagga tagtctactt ttaccgcctc gcgttctgct gcgatttgtt
     6361 ttggatcttt ttctggctta gataaattga ttgtgatttc cagttcacca tactccgact
     6421 ctgccggaaa tttgatgttt tttgcgatgg ctcttgtttt gatgtagtca tttttcttac
     6481 ttaaattaaa gaaatactca gttgcttcct ctgggctttt cgaatagtgc tgtgcaaaga
     6541 atgcattgac gactgaaggt ggcggtgtga gaaaatccat taattgcgct tcaaaaatct
     6601 cttgttctgc tggcaattct gaaagaacac cattttctct tgccttagcg acaagctgat
     6661 ccaataagtc tacagattct tccttaactg gtcttacttc aatgctttct agagcatctt
     6721 ctcctaccat ccctaaaaga cgattctgta aatataagcg gtccatttcc atccatccgc
     6781 cagcttcgat cgcaagtgtg gcaaaatcac aaatcgtttg actgattgac atatctcatt
     6841 cccctattct tcataaccat ttggatggct gacatgccaa tcccatgctg ttttgataat
     6901 cgctttgata tctgtgtatt caggtttcca gcccagaatc tctctcgctt tttcacttga
     6961 agcgactaat cggctaggat ctccagctct tcttggtgct atttctgctg gaatttcttt
     7021 tcctgtcact tctctagctg cttctagcat ttccttgact gagtatcctt tgctgctgcc
     7081 taaattgaag aagttgcttt catttccttc tttcaagtat tccaaagcta aaatatgtgc
     7141 agcaatcaaa tcttctactt gaacataatc tctgatacat gttccatctg gtgtatcata
     7201 atcgtctcca taaacagcca aggcttttct ttgaccaaga gctacttgta aaatgattgg
     7261 gacaagatgc gtttctggtg tatgatcttc tccgatagat gcatctgctt ttgctccggc
     7321 tacattgaag tagcgcaaag cgacatagcg catgccatat gcttggtcac accacttcat
     7381 cattttttcc atcatcaatt tactttcacc atacggattt ttaggattag ctggtgtatc
     7441 ttctgtgatt ggagactctt tcggctctcc ataagttgct gcagtagaag agaatacgat
     7501 tttattcacg ttgaactcgt gcatgacttc aagtaagatt tgcataccgt atacgttgtt
     7561 gttgaaatac atcaatggtt tttcgacaga ttctcctact aaagagcttg cagcaaaatg
     7621 gattactcct tcaatcggtt ccttttcaaa tacggaacgt agaaattctt tatcccggat
     7681 gtctccttca taaaaatgag catctggatg tactgcttgt ttgtgacctg tgagcaaatt
     7741 atctaccaca gctacttgat accccttttg aactagctgg tctacagcgt gcgaaccgat
     7801 ataaccggct ccgcctagta ctaaaattgt cataaaaaac actcctttga ttagtaaaat
     7861 tgtgtttgtt gattctatat aatttaaaaa acgaagttta cttttatggt gaaaacaaac
     7921 aaagtaaaat ttcgtaaatt catatggtaa atttataata aaacatttag taaacatttt
     7981 caagggtttt ttgcaaaggt ttacaaacaa acgtaaaact agcctttttt tctcttttgt
     8041 tccataatag atataacaaa taataaatga ggtggattca atgaaaaaat ttgtatctgg
     8101 aatattagta ggaagtctgg caacagccgc tgccgttgct ggtttggttg cttctgtgaa
     8161 aaaaacagtg atcgatccaa ttgacgaaaa agaagcaatg atcgaagaaa accgtaaaaa
     8221 agcaatgcgt aaacgcgttt ctcgttaata attatttaaa aatacgcatg aagactttct
     8281 aaaaaacaga taagtcatca tgcgtatttt tatttgtcag tcaatataag ttgtgctatt
     8341 ttatctgcct gtctcagatt ccatggatct gtataaggtc tagctactgc tgcagttagc
     8401 caatccacac cttcaacagg tattccatag caataaaagt cagactggat ttcccctgaa
     8461 gcatcaatca cgcgattcgt acctcgctca acatctatag cttctgaatg ataacttcct
     8521 gtattatctg aaaatgaaaa agggcgaaca agtttgtttt ccttcaaatt ttttatgaca
     8581 ggattacttg aacgagaaaa gcttgtttgt ggcaatcgag cttcaatcaa atagcgactg
     8641 ctgatttctt tatcttgtag agaaataaca tagcggttgt gcttttgctg aatatcaaat
     8701 ggcggttcga tcaaatgaaa aatacccgca tctatcagtg ctgctaattc tttcgtccga
     8761 atgattggcg gaccgatcgt taaaaagcta tttagatgag taaaccatcc ccatagtatt
     8821 tctttgtatt ccttagcaga aaaaacgtgc cattcaatag cctgccggat tggatcacgc
     8881 cagtctttta atgcatcaaa tgttgaagca aatgctcctg tgcaattgcc tttttccgct
     8941 tcttttatct gcccttcaat aaatattcgg ctagcttctg aaaagggctc attctgatag
     9001 gcaagaggag tttctaaata cgaccaatcc catctataag ggattagtgc tggatgcttt
     9061 ccgagacact cctctgaaga gagggagaga aaatcatgct caaaattttt ctttggaatc
     9121 ggcaagtgat attcttcgat gattttcttg taataaaaca actcaacgtc ttttttcaga
     9181 tagtcaaaaa atgtttcgcc agtcaaaaat cccgactgat gccattgctc caggttttct
     9241 tttgttagta accgtggcca tgccattgct ccgccttgct tttgatttct tcctcgaggg
     9301 taataaggga gcccccttct tgatccactg tatacaatcg gttccttgcc tgatggatga
     9361 tagaccagtt ttcccttttc gttctcgaag cgccctcctc ttgatactgt aaacaatccg
     9421 acataatcaa aaaaacttaa gcctagacct cttaaaaaga cattttcttt aggcggtatc
     9481 ttcgaaagat ctacatctgc aggattagaa ggaagctgat acagtaaaac atttttcttt
     9541 gcgtagtcta cccacactct ttcttcttct gtctcatcat tttcccagtg accgcttgct
     9601 aaaacaagct gatcgataga atagctttgc tgatctgtct gtaaaagata accatctttt
     9661 cttttgctta ctttttttac taaagaataa acaaactgaa tagaagctgg aaattcttct
     9721 tgcaactttg agaaaaacca gcgttgatag agtccgtata aacatcgagt agattgctga
     9781 tctttttcca gacgattgat ttcttctata attagttgct tatttttggt taccgtctgt
     9841 tcgatataat ctgtagcagg tcctttactc cattgaaaca agttgggtcc ctgtgttacc
     9901 aatcctccac tggacaatgt ttcatctgta aaaagcgtca cttgttgaga aacagaattc
     9961 attaataact ccggtggctg attcaaccgc caaactcgac ctccaggacc attgggatcg
    10021 aaaagaaaaa gttcaagaga atgtctgtcc tgattattat ggagaatcct ttctgctaca
    10081 cttaatcctc taggtccggc accaatgatc cctattttca tttgcatgcc tccatttcga
    10141 taaatttttt caataaaaca gcatggttct gatgttcatt ttttgcacca taaacaaaag
    10201 taatgactgg atgttctttc ataagttgac tgagtttaaa aatagcttgc ttcttttctt
    10261 cttcgaaaat caattcttgc tggtatttct ttgcaaatga agcaaatcgt tctggtacat
    10321 ggtcaaaagt tttgcgtgct tcattactag gtgcaatttc tttcaaccac agatctaact
    10381 gcgctttttc ttttgaaatt cctcgtggcc agaatctatc cacgagtatt ctatagccat
    10441 cctctttttc agcatcaagg taaacacgtt ttaatttgat catagttgtt tccttctttc
    10501 taccttttcg cttagcttat atatgtagat aatagcacaa aataaaaaaa atgaacggca
    10561 caactatcca ttgcgccgtt catgtgatct tggtcaaaat ctaatgattg attcggtttt
    10621 taaagctgtc atacaatcat ctgttttata cttttttact tttgattttt ccagaccatc
    10681 cgtcatatcc acccttaagt aaataaatct cttgataacc tgctttacgt aatttatttg
    10741 ctgtacggat gctcaaactt ttcttttgat cgtaaatata gacaggctgg tcttttcgaa
    10801 tagatcctaa tgaatctttc aaaacagtgt aagggatgtt tcttgcacct aaaatatgcc
    10861 cagcatcaaa cacatctttt tcgcgaacat cgattacttg tgcttttctc attccttctc
    10921 gaaattcttc ttctgtcaaa gtcgttgctg aacgtttggc cattactcgt agatagactt
    10981 cccaaaggat gattgcaagc acaatcaata gcaaaatgat attaattacc cataaaacca
    11041 tgtaatgcct ccgttttttt aagcaaattt caaggctaat gaggcagcac caatgacacc
    11101 agcttcatta ccaagctctg ctaatttgat ttttgttgat tctcttactt gcgggaaagt
    11161 aaattcttta aaatatttct ctacacggct acgtaaaaat tctcctgctg ccgatactcc
    11221 tccacctaac acgatacttg acggatttaa tgtattcccc aagtttccgc aagctaaacc
    11281 taagtagaag catacgcgat caacaaccat caatgcaaac ggatcgtctt cttgagccat
    11341 ttcaaagata tctttacttg ttacttcttc tccgttgtct agcattgctt tcagttttga
    11401 atcccctgcg tattcttctg ccaagaaacg acctaggcgt accacacctg tagcgctaga
    11461 tacagtttct aaacatccgc gtttaccgca agtacattcg aacccttcag gatccacagt
    11521 gatatgtccg atttctcctc cagctccgct cacgccgtga atcaaatttc cgccagcaac
    11581 gatacctccg ccaacacctg ttcctagagt aacgaataca acgtctggat cgttttctcc
    11641 agcacctttc catcgttcac ctaaagcagc aacattcgcg tcattatcga tagcgaattt
    11701 gattcctgtt cctgcctcga tcagtttttt cacaaattga acttctgtcc agtttaagtt
    11761 atacgcacca ataaccgttc cagcttcgct gtccacgctt cctggggtac ccatgccgat
    11821 tccgataaag tcttcggctt tcatattata taaattcaaa cgatgattga ttgattcaat
    11881 gatttcagga acgatatgct taccatcatc caaaatattg gtatcgatgc tccatttttg
    11941 ctgaatctct ccttctggtg ttaaaattgc aaattttgct gttgttccac ctaaatcaat
    12001 cccaataatc ttcttgtcca ttacaaattt cctttctttc ggctttcttc aattcggtgc
    12061 tctcttttta atatgcgcca cgctgtcatg tatgtatcat gatcaatcaa acggctatca
    12121 tacagattac gcaattcgat agtcattaat tcaatgtcat agatgcgtgc acccacatag
    12181 ataaagatgc caaatttttt gaacagctgc tgtacatcat ataagctctc catctttaag
    12241 cacttccttc atattataca agtaaaccat atttttttaa acccagtaaa atacatatta
    12301 caaaaagaaa tacaaaaact agtgtagaaa gaatcctttg gtgtatcgaa tagcttgtct
    12361 gttgtttcgg tagacccaaa acattcccta acactaatcc gccaattagc ccaccgacat
    12421 gtccccaaac atctacagag gtatccagaa gaccaaaaac aaatgtcaac acaataaata
    12481 aaagaaaatg tctgactagc tgctggatcg ctgggttatg tttaaaatga aaccctaaga
    12541 taaacaacgc accgaacaaa ccgaatatcg aagtacttgc accaccagac agaacattcg
    12601 cttcgttaaa agcaaagctt gctgtatttc ccattatccc gctgaacata taaatcaaga
    12661 aaaaacgcca atggccatag attgcctcga tttgctgccc cataaaatac agtatcagtg
    12721 agttcattac gaaatgcatc agtccaaaat ggatgaagat tggtgtcact aaacgccacc
    12781 attcattgaa gtgaacaaga taaggaccat acattccggc ataataaggg atctcaagtc
    12841 gcggaaataa ttccatcatg aggaagaccg ctgtctgtat ccctaaaaaa gcataggtcc
    12901 ataacggtcg tcttaaccat gcacgcaatt ttatttgctg ttgataattc atgaaaacct
    12961 cccatttcat tttagtcaat aaacaattgt tggatcttct gatcaaagct ctccggttgc
    13021 cattgttcat tcaattgttc acgaaaaaca aggctacatg tttttttatg aaaattattt
    13081 aaataccggt cgtaataacc accgccaaaa ccgattcgat aaccggctct attgtagata
    13141 atacctggta caatgaggag atcgatcatc tccggcaaat agagcttctc tgattgtggc
    13201 tcaagaacac caaaactcga acgtacaaga acggactgtt catccatttc ataaaacgcc
    13261 atctgacgtt tcggcaaagt tttaggtaca actattttct tttgttcttt ttctgctcgt
    13321 tggataaccg gctgtgtatc cagttcaaat tcattcgata aagtcgtccc tatcactttt
    13381 gctgacttcc agtactcgct ggcgaatagc tgtttataga ttcgctgttc tttttccttt
    13441 ttcttgccac ttttggccag atttttcagc ttttcgattc ctttccggcg taatcttact
    13501 ttcaattggt ccaaaaactc acccttcctt ctctcataaa atggtatagg aaaaaaagaa
    13561 aaatagcaat cgaactttca aataaatgca taaatagaat aaaaagaaga cctagaaatg
    13621 attggccttc cttttttaat gaatttcaac tgttcagcgc gtgtttttgt ctgataaaaa
    13681 gtgcatacag aaaatatggc gcaccaaaaa gggcaatcaa aattcctgta ggtacttccc
    13741 cacttggtag aatcactcta gcaaccatat ctgcactaag cagaaaaatg gaaccgaaca
    13801 aaccgctcag aacaaaggtc cagttatttt ctttacgtac gactagcttc gcagcatgag
    13861 gagctagtag tcctacaaaa gataaactac ctgtcaccgc tacactaccg cttgccaaag
    13921 caacagctaa caacaaaagg attttttgac ttttagccac gttaaggcct aatccttgcg
    13981 cgcgttcttg accaaatgat agcaatacaa tcctctgtgc ataaagtcct cctagcggaa
    14041 ttaagataac cgcccatgga agcaatgccc aaacataagg ccagctcgca ccccagattg
    14101 atcccgctaa ccatgcattc acaaatccat aattttcctt agataattgg atcgtgcata
    14161 gcaatgttaa tgcagaaagg ccggcattaa cggcaatacc ggacaataag attcgattca
    14221 tacccaaaaa gccatttcga tcataggccg caagatagac acagcaagca gctaaaacac
    14281 cacctagaaa ggctacaact agcaatagaa acggcagcga atagtgcgaa aagaaaccta
    14341 aataaagcat cactaaaaat cctgctccgg cattaatgcc caatatacta gaatctgcca
    14401 attcatttct tgttactcct tgcaagatat aaccagaaac gccaagacaa gctcctgcta
    14461 ataaactgat cagtaatcga ggaactcgaa attctctaac aatcagcgcc aaagaagatg
    14521 attctttccc tatgaacgtg gcaaaaagct gatccaggta aataaaaggc gtaccaatca
    14581 ttaaactacc tatagacagg atgacaagta caaaaagcaa gaaatagaac gtccaatata
    14641 tttttttcac cgttgttccc tccttacttg tatcagtaaa aacggaacgc caatcaatgc
    14701 aatgactaat ccaaaaggtg tttcaaaagg gggattgatc atcctcgctg taaaatccgc
    14761 caacataacc aatccaccgc caactaaaaa agagacaggt agaacataac gatagtccgt
    14821 tccaaccatt ccgcgaacaa ctggcggaac caacagccca ataaatgaaa caggtccgac
    14881 taacgaaacg gcaatggctg ctaacactgc aactagacag aaaacaatca accgcgttcg
    14941 tttaggatga cttccaagag ctaatgcatg tgtttcactc aaatgaatca aagatattgc
    15001 ttttcctaat gaaaaagaaa ccaaaacagc aagtatatat aaaggaaaca atactgctaa
    15061 ttgtttccat gtgatattgg cagctccgcc tacaaaccaa aaagcaagat cctgctgcag
    15121 attaaagaat tgggtaagtg cttggctgat agcagtaaaa aaagaactaa tagctacgcc
    15181 agctaaaatc agctgtaaag gattcatgcc cattttcgaa aactgcaaaa aaccaaatac
    15241 gagcaaagtt acagcaaaag aacctgccaa tgacattaga aacacagaaa aatcagaagc
    15301 tttaggccaa aaagcaaaac taagggccat tgccaacgaa gcacccgcgt tgattcctag
    15361 taagcctgaa tctgctaaag gatttcttgt caccccttgc atcaatgcac cgctcaatgc
    15421 aaaacaactc cctacaagaa atgctcccgc cacacgaggc aaacgaatat ttcgtatgat
    15481 ttggtgactt tgttttgatc tatcaaaatc aaacaacgcc tgccaaaaat ctgctttttc
    15541 cacagtcgct gcgccatttg aaagagcaac aaaaaaaata atcaaaagaa caaaacagac
    15601 caaaagcagg agtctatttt tcttcatagt cctcataatt gatcctcctg ctcttttggc
    15661 aacaaatcat aggttagaaa aactggttta tttgttcttg gaagcgtgac aatcgttgca
    15721 tcaatctcaa atattttttt catacactct tttgtgatca cttcttcagg tgtaccttct
    15781 gtgatcaact taccgttttt cattgctaat aaataatctg aaaaacgtga agcatgatta
    15841 atgtcatgaa tcgacatcaa gatcgttgtt tgtttcattt gattgatttc agtcaataac
    15901 tgtaggatct ctaattggtg tgccggatct aaaaatgttg tcggttcatc caagatcaag
    15961 atatctgttt cctgagcaag cgccatagcg atccagacac gctgggtttg tccaccagac
    16021 agcgttgcga tatcccgcat acgaaatcca gctagacctg tagcatccaa cgcccactca
    16081 attgcttcat gatcttgcgt gtccatgcca ctgaacagtt tttgataagg gtatcgtccg
    16141 taagatacta cttcttctac tgtcatacct gtagggtgtt cactagtttg tgctaataat
    16201 gcaatttttt ttgcaagttc ttttgaactg tatgtagaga tatcagagtt attgacaatg
    16261 atctttcctg atttcacagg tagtatacgg gtaaaacttt tcaataaagt agattttcca
    16321 cttccatttg gcccaatcaa actagtgatt ttcccttcag gaatcgtaac agacatgttt
    16381 tcaatgattt ttttgttttc gtagccggct gcaatgtcag ctgctcttaa tctcgtcatt
    16441 tcacccatcc tttcgctgaa aatcattctc aattatgacg ctgtttcaac atttcgtcaa
    16501 gcgctacttt catatctata gccaaatgaa gcggttgaca gcgttcgaaa tatcaggaat
    16561 aataaccaat gagaaagatt ctcaattatg ttaggaggaa agaaatgaaa ttaaaaacaa
    16621 aagtattcac attatctagc atattgctgc tggctggagt actgagcgct tgctccccct
    16681 cttcagataa agaaacagct acaactgcca attcgacgac ttcatccaat agtactcata
    16741 cagtcacaga tacacttgga cataaagtag agattccaaa cgcgcccaaa cggatcatcg
    16801 gcagttactt agaagactat ctgattgcac taggggaaaa acctatcgcc cagtggactg
    16861 ttggcagtgg ttccattcaa gattatctgc aagatgattt aaaggatgtc ccaacgattt
    16921 cttatgacct tccttatgag aaagtgttaa gctttgagcc tgatttgctg ttgatttctt
    16981 cttccgctac tgtcgaaggc gggaaatacg aacaatatag caaaatcgcg ccaacttatg
    17041 ttgtaaaaaa cggaacagat gtcacatggg aagaacaact aaaagatgtc ggcaaagctt
    17101 tggcaaaaga aaaagaggca gaaaaaatca tttccgatta tcaaaaatcc atcaagaaaa
    17161 ctcgagaaga actagcagat aaaatcgaga acaagacagc tgcagtgttg tgggtgacca
    17221 acaactcagc ctttatggtc agcaaaaatc ggtcaagtgg aagaatcgta tacgacgatc
    17281 tggaatttgg tgttcctgat ctagtggaag aggtctccaa aaaagccact tctgactggt
    17341 ctgctgtttc ctctgaaaag ctagctgaat tagatgctga ctacatcatc cttgtgaaca
    17401 gtgacaaaga tgctgccatg ttcaatgaac caagctggaa gaatctaaaa gcagtgacag
    17461 acaatcatgt actgcaattc ggacctgaat ctagttggct atataacgga cctattgcca
    17521 gtcaggaaat ggtcaatgat atcaaagaaa aactaaattg attttaacta aaaaaagcat
    17581 cttcacgatt agtcgtgaga tgcttttttt cttattaaca aactagacgc atagatttta
    17641 ttcttctttt gaagcaaccc tattttttac tctttcccat ttttgaaaac gatgggcata
    17701 tagattttct tgatccgtta tcccctcata ctcctctata agggcgaagt ctgaaaaatc
    17761 aatattccca atatacgtat cgccttcaaa cgtgctatcg atcaatgttc gccagatccg
    17821 accagtgtaa ggaagaagtt cttgaaaaat ccgactacca cccgaaacat agatatcttc
    17881 tgagatcgtt tgactatacg acaaaagttc atcgatcgaa tgcattactt ccgcattttc
    17941 tttcgtgcga aaatttggtt gcgttgtcaa cacgatgata tggcgataag gaagtgaaag
    18001 atcccccatt ccttcatagg tctttcttcc catcactaaa attcgatcca ttgtatgttc
    18061 gcgaaagaaa cgcatatcat ttggcaatcg ccatggtaac aaaccatttt tgccgatcag
    18121 accattttta tcttgtgccc acatggaaat aaacatatta tccgctcctt atgattttag
    18181 atgttttgag ataaaaaaag tctcaattct ttgaccagca aggaattgag aactttaaaa
    18241 acattatttt gtttcacggt gcaaagtaac tttgcgttcg cgtgggcaat atttttgttt
    18301 ttccaaacga tcaggattgt tacgtttgtt tttgcttgtt agatagttac gttctttaca
    18361 agaagtacac tctaaagtga tgtttacgcg catgtttttc cctcccattt ttacaagaat
    18421 cctctaagat gactctcaga cttgattatc ttaccatgat tttttctaga ttgctagtgt
    18481 ttttcacgat tttgttaagt aaaactactt gacgataaat acaacggttt tacttctcaa
    18541 acaaaatctt tttctttttg tataaatacc cttacacttg tttatataga ttatgttttt
    18601 taataaaggg ggatactaat atgccatgga acatgaagga ctttcctgca tcaatgaaaa
    18661 acttagataa attgacaaga aaaaaagcaa tcgacatcgc aaatgcatta ttagatgaag
    18721 ggtaccctga taaccgtgcg atcccaattg ccattgatca agcaaaaaaa tggtatgaag
    18781 atgctagtga atcagaaaaa cgaactttcg aaaaagaaaa aaatccaagc aagacggatg
    18841 aacacgatac gaatcctcgt gctggaaaac tgcttgattc agatgtgatc gtagaatatg
    18901 aggaagaaca atggatcgtg aaatctaaag gggcaaaaaa ggcaagcaac cattttgata
    18961 caaaaaaaga agcaatcgaa aaaggaaagc aagtagcaca aaacaaagaa tcaactttgt
    19021 tcatttataa aaaagatggg acaaaagaaa aagaaatcga ttattgaaca tatgtaaagg
    19081 ggttgtgacg aaaatcgtgt cacaaccccc ttatgttcga aacaatggtg ttcctctgcc
    19141 aactccgaac cataagtcat aatatctata aaacgggaag agctgttttt tgaaattgtt
    19201 tcttatcact cagtgttaaa cggtaggaga aaacctcttt tttattgata tttgattttc
    19261 atcaaacagt cacatagtcg attgcttctg cttgcggaat cgtttcatct acaaagtaga
    19321 ctttatacca tgtcaagaaa ctgaagattg tccagatttt tctttgctct tctttacgtc
    19381 cttcgtgatg gtcatctagc aacttcatga ttttttcttg atcaaaaaat tcttttgcaa
    19441 aatctgcttc gaataatgct ttgacttgtt cgtagccgtg ttcttctttc aaccaagctt
    19501 tgattggcac agggaatcct aatttcacac gattcgacca ttcttccgga agatgtttgt
    19561 tcgcagcttg tctgaaaata tcttttgtgt tgttttgatt caataaaaac ttacttggta
    19621 tttgttgagc caatttcatg acttcgatat ccaataaagg tactcggact tccaaagaac
    19681 ttgccataga aagtttatct gcctttaaca agatatcttt tggcatccat tgatgaagat
    19741 cgacatattg catcttattg acttcatctt tgattccttc tgttttctcg taatgaacag
    19801 tcatgatttc atccacagat ggtgctgttt gatattttgg tgttaacact tctgatgctt
    19861 ctgattcttc gaacactcga gcatgaccga taaaatattc ttttgcagga gcaagtgatt
    19921 catataaatg gatccgtcca tggaagtttt tcatttttcc gattttacgt cctaaattat
    19981 agcgtgttcc ttttggtaat tttttcaatc cttgagcaat cacacggatg aattttgaat
    20041 tcgtatggaa tccatacgtt tggtatcccg caaacagttc atccgcccct tctccagatt
    20101 ggactacccg cacactttgt gaagcaagat ttgctaaaaa gtacaaaggt acgcaagaag
    20161 gatttgaatc aggttcatct aagtgatact ggatcaaagg gaatgctttg aacgacatgt
    20221 ctccgtcgat cactgctgct gtattatcta agtccaatag ttcggttagt tttctggctt
    20281 caatcgcttc attataggtt ttatcatcaa acccgattga gaaagaatga tctggtttca
    20341 atacagaggt tacataactg gaatcaaccc cgctggataa gaaagaacca acttctacat
    20401 cactgaccgt atgtgcttca atagaagctt gaacggtttc gtcgattttt tcgatccatt
    20461 cttgtcggga atgcgtttct tttgtttcaa aatctgcatc ccaatattgc tgaatgtcta
    20521 tttttccatc tttatacgta taataatggc cttcaggcaa acgatagaca tctttgaaga
    20581 atgtttcgcc gtttaatgga gaatattgga aagtcatata aggtttcaac gcttctttgt
    20641 tcaattcttt gttaaaatca ggatgaggca gaaaactttt gatttctgaa ccaaacataa
    20701 atgttccgtt catttctgca taataatatg gtttgatccc aaagtggtct cttgcaccga
    20761 ataactcatt cttttcatta tcccaaatag caaaagcaaa cattccgcgg atcttttgca
    20821 ataattctgt tccccattct tcgtaaccat gcaaaaggac ttctgtatct gcatgtgttt
    20881 gaaaaacgtg gccagctgca atcaattctt ctctcaaagg ctggtaatta tagatttcgc
    20941 cattaaaaat aatgatcttt gtccgatctt cattgtaaat aggctgagta ccgccctcta
    21001 agtcaataat acttaatctt ctgaatccta aagcaacatg tttatcgata tattctcctg
    21061 aactattagg tccacggtga acgattcgat ccatcattgt attaataatt gtttttttgt
    21121 tgtccttgtc gttaacaaaa cctacaatac cgcacattta actttcacat cctaatataa
    21181 aattactgtt tatttcattc atatgcatct tatcatagaa taaatagcgg acgaatattt
    21241 tctctcttac aatttcgtca cgtgaaaaca aaagatgaaa gtttctgttt ttacagaaaa
    21301 taaagtccca atatctaatt taccatgttt tcttttttga ggctacctta ttctcttcgt
    21361 gttaaaaaaa tattagtgga tgctatttcc aaaaaaacca gcaaaaaaag acacgaccta
    21421 aagatcgtgt
//
LOCUS       DAJSBL010000043        21362 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.043, whole genome shotgun sequence.
ACCESSION   DAJSBL010000043 DAJSBL010000000
VERSION     DAJSBL010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21362
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.043"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..83
                     /locus_tag="ONT85_001936"
     CDS             <1..83
                     /locus_tag="ONT85_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HCR2378905.1"
                     /translation="SVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            74..859
                     /locus_tag="ONT85_001937"
     CDS             74..859
                     /locus_tag="ONT85_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spo0B domain-containing protein"
                     /protein_id="HCR2378906.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDGGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            828..1544
                     /locus_tag="ONT85_001938"
     CDS             828..1544
                     /locus_tag="ONT85_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HCR2378907.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     QPLTCYELTKNGRKKLTDYC"
     gene            complement(1646..2680)
                     /locus_tag="ONT85_001939"
     CDS             complement(1646..2680)
                     /locus_tag="ONT85_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HCR2378908.1"
                     /translation="MEEESMRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIV
                     SAKDHDAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVP
                     SYSPNAIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRI
                     GLTEAVLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQ
                     MINEGFLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSF
                     KPWETIPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNE
                     VSLPKARQLR"
     gene            complement(2684..3742)
                     /locus_tag="ONT85_001940"
     CDS             complement(2684..3742)
                     /locus_tag="ONT85_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HCR2378909.1"
                     /translation="MNLFDIFRSTLTDMSIVSAITSTVFIILLGFVCRKKGIFSESVG
                     KILSKVVLTVALPALAFRAFMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYK
                     GDKQTTLRVLTIFGSTTFFGTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGL
                     KMEAKNIKAMFVNPIIIATFAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPW
                     LFQPMDYLAGLSSPLAWLAIGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILA
                     VLTATNILPVNFEALATIVIMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVL
                     TPVWIVVLQMIHGIGLFS"
     gene            complement(3962..5110)
                     /gene="nagA"
                     /locus_tag="ONT85_001941"
     CDS             complement(3962..5110)
                     /gene="nagA"
                     /locus_tag="ONT85_001941"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HCR2378910.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(5186..6022)
                     /locus_tag="ONT85_001942"
     CDS             complement(5186..6022)
                     /locus_tag="ONT85_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR2378911.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(6206..7423)
                     /locus_tag="ONT85_001943"
     CDS             complement(6206..7423)
                     /locus_tag="ONT85_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HCR2378912.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            complement(7572..7751)
                     /locus_tag="ONT85_001944"
     CDS             complement(7572..7751)
                     /locus_tag="ONT85_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2378913.1"
                     /translation="MGGLIKMEKYDGEFSGLGMILGVLIGLAFGRFLLGFMLGIICGI
                     AMDWAANLWNDYHDN"
     gene            7806..7889
                     /locus_tag="ONT85_001945"
     tRNA            7806..7889
                     /locus_tag="ONT85_001945"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7840..7842,aa:Leu,seq:gag)
     gene            complement(8129..9499)
                     /locus_tag="ONT85_001946"
     CDS             complement(8129..9499)
                     /locus_tag="ONT85_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2378914.1"
                     /translation="MNKHNRKITNNKELILLLIGIWGIFLLFNMYMPTMRADDVVYAS
                     RLDELGYLGASIEHYKTWSSRIIIELFLMFFSKHFMLWKLLNSTIMLGIVVLLCKYVF
                     EKINVKNLLLVCSIYCLIPLTVMGETGWRATTLNYQWPVAFSLLTFYPFFQLLRGEEI
                     NRKIYWVSIPLLIFLTNQEQVNACFFVLTSIVSLYLIVNGRYNYKLSVFSIISLAELI
                     FSLTTPGNALRAAHEINKWFPEYKNFNFLNKLDLGISSFGKPFFLDMNILFLLLFFLI
                     FLLTYRKCQNYYVRILTALPFFLNLIIYFGNTMGQSFTYVNGNKRAMIWSSSNLNNLF
                     TELGTKLSLFYPGTWIATLVVLALLLCLIVGIYLSFDNKKTSIFLVILMIMGFCSRLI
                     MGFSPTVWASGMRTYYILYVVIAILVLMAVKELMKSMSVQKNEFMQFGLTVLGICTFI
                     ITVINR"
     gene            complement(10213..10503)
                     /locus_tag="ONT85_001947"
     CDS             complement(10213..10503)
                     /locus_tag="ONT85_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2378915.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(10711..11403)
                     /locus_tag="ONT85_001948"
     CDS             complement(10711..11403)
                     /locus_tag="ONT85_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HCR2378916.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQALTDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
     gene            11798..12421
                     /locus_tag="ONT85_001949"
     CDS             11798..12421
                     /locus_tag="ONT85_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HCR2378917.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNSISDINLIYSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQP
                     VVQETVQTEKQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYI
                     GRYQLDSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
     gene            complement(12505..13227)
                     /locus_tag="ONT85_001950"
     CDS             complement(12505..13227)
                     /locus_tag="ONT85_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HCR2378918.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(13217..13954)
                     /locus_tag="ONT85_001951"
     CDS             complement(13217..13954)
                     /locus_tag="ONT85_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR2378919.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            complement(13966..14958)
                     /locus_tag="ONT85_001952"
     CDS             complement(13966..14958)
                     /locus_tag="ONT85_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR2378920.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            complement(14973..15716)
                     /locus_tag="ONT85_001953"
     CDS             complement(14973..15716)
                     /locus_tag="ONT85_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR2378921.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     regulatory      complement(15790..15894)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(15957..17279)
                     /locus_tag="ONT85_001954"
     CDS             complement(15957..17279)
                     /locus_tag="ONT85_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCR2378922.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     gene            complement(17266..18231)
                     /locus_tag="ONT85_001955"
     CDS             complement(17266..18231)
                     /locus_tag="ONT85_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR2378923.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            complement(18228..19766)
                     /locus_tag="ONT85_001956"
     CDS             complement(18228..19766)
                     /locus_tag="ONT85_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HCR2378924.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            complement(20182..20469)
                     /locus_tag="ONT85_001957"
     CDS             complement(20182..20469)
                     /locus_tag="ONT85_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2378925.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            20603..21271
                     /locus_tag="ONT85_001958"
     CDS             20603..21271
                     /locus_tag="ONT85_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HCR2378926.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
BASE COUNT         6480 a         4342 c         3532 g         7008 t
ORIGIN      
        1 aatcagtgat aatctatatg acgttttgtt taaagagacg attgagcatc aaacgaccgt
       61 tcggactcaa tccatgtatt taaatgggat agaaactatt tctagcgtta cacctctttt
      121 tagcaatgac cgttttttag gagcagtcgc aactttacga aatcgatcag aagtaatgaa
      181 attaatggag gagcttgggg ggacgaaaca atatataaac tccatgagag ctcaaaccca
      241 tgaattcatg aataaacttc atgtcatttc tggtttgatt gatttgaaga agtatgaaga
      301 agtcacccat tttatccagc agctaaatca aaattataaa aaagagattg gccaagtctc
      361 aagtctgatc aaagtacctg caattgccgg ttttttatta ggcaaaataa acgaagccaa
      421 agaacaatct gttgaaatgc tgttagaaag cgattctttt atccctaaaa tggagatgga
      481 tgactccatc cattcacttt tgcaagtgtt agggaactta cttgataatg caaaagaagc
      541 cgtgctacaa aaacatgaaa gaaacggaca ggtccggatt catttaaact atgacacaga
      601 agctgaagta ttcatcgtga ccgtctgcga tggcggtgtc gggatatcag aagaaatctc
      661 agaaaaaata tttacgctag gtttttcgac gaagggaatc aatcgtgggt ttggattgaa
      721 tctcatccat tcaatcgtca aacgccacgg tggattttta gaattttcca aacaaacaaa
      781 tggcggtaca atcgtctatc tagaattacc aaaaaaggaa gatcaaaatg atgacaaatg
      841 tcctgataat cgaagatgat ccaatggttg cagaattgaa tcgtactttt gtggaaagca
      901 tccctaatat gaacgtaatc gaaagtttca gaaatgtaga agaaggaaaa aaatatttca
      961 gacaaaaaaa cgcagatctg ctgctgcttg atgtatacat gcctggcgaa agtggtatcc
     1021 attttttatc ttggcttaga cagcaaggaa atgatatccc agtgattttg atcactgctg
     1081 cagatgatat cgaaaccata aagaatgtga tccgctacgg tgccttagat tatttgatca
     1141 aaccgttcac tttgcaacgt tttcagctgg cgatcgaaaa gtatcttgct ttctctcaga
     1201 caatccaaac gactgaaaaa gccgatcaga aattgatcga tcaagtattc aaccacacca
     1261 ctcccattca cgaaatacca tcattcatcg atttgcctaa aggtctgtca aaactgactt
     1321 taaaaaaaat aagtgcattt attctaaaag aagatactat tttttcaaca aaagaactgg
     1381 ctcaaaaaag ctcactgtca cgtatatccg tcaagaaata tttgaatttt ttagtagaac
     1441 aagatatatt gaacgaaaga atcaaatatt tggaaatcgg ccagccgcta acctgttatg
     1501 aattgacaaa gaatgggcgg aaaaagctca cagattactg ttgagtcatc gaattatccc
     1561 atacaaaata gtccaagtat acagaagtaa caactgttta cttggactat ttttaaacat
     1621 tttattgacg aatatccatg ttttcttatc ttaattgacg ggctttagga agagatacct
     1681 catttttcgt ttttccagtt tctattattt cacgaaagtt ctcgaaactt gtcgaaatca
     1741 tatttgataa tgcctcatct gtatttgaac ctacatgcgg ggttacaaga actctagggt
     1801 acatttctac taattgctgg atagcagggt ctggaatcgt ctcccatggc ttgaaggatc
     1861 ggaaaaaaac ctctgtttca tttgcaaaaa catcggttgc aaaaccagct aaatgattgc
     1921 ttttcaaagc tcttaaaata gcttcattat cttgaagctc tccacgggct gtattaatca
     1981 agatgctacc ttttttcatt tgagctaaaa atccttcatt gatcatttga tcgttttcac
     2041 ctgggatatg aggtacatga atactgacaa tatcgctttt ggctaataac tcttcaagcg
     2101 agtggaacgt caatactttt ttagcagctt ctgtctgatg aatatcgtag ccgatcactt
     2161 tggctcctag tcccttaaac aaaacagctt ccgtcaaccc tatcctgcct gttcctatga
     2221 tccctactgt gcaatttctg atttcacgac taaacatttg ctcatccact cgaaaatctt
     2281 tgaaagatgt tcggatcgtc gtatacgcag tatttctcaa aagcatcatg gcaaaagtca
     2341 aagataactc tgctatcgca tttggcgaat acgatggtac acgggctact gtcataccaa
     2401 aatcagctgc cgcttgtaga tcgatatgat tgactcccac agtccgtgta aaaacataac
     2461 gaatcccata ttcctgcatt tttgcaagat tggtctcatc tgctatacaa tttcccctca
     2521 acaatacagc atcatggtcc tttgctgaca cgatattgtc atgtgtcaac agttcttcat
     2581 aaagactgag atcataatgg taaatattca atttttcaaa aaaatctatc tcgttcggac
     2641 gaacaccgta acatgcgatt ctcatacttt cttcctccat ttcttatgaa aataaaccta
     2701 tgccatgaat catttgtaat acaacgatcc atacaggtgt cagtaccaca gcaaaaatag
     2761 ttgacagtaa ggatgcattg gaggctaaaa gtgcctccct atcaaaactg atagcatagg
     2821 ctgctgccac tgttgcagct ggtgtagcca tcatgataac gattgttgcc agtgcttcaa
     2881 aattgactgg tagaatattc gttgccgtca aaactgctag aatcacgata ttcaatgcag
     2941 gtacaaatac cactttgact agactgtaat accatgaagt tttttcagat gcagcttttt
     3001 tgaagcttac ttcacctaat gttgcgccga ttgctagcca cgccaatggt gaacttaaac
     3061 cagctaaata atccatcggc tgaaatagcc acggtgcagt ctggtcaatt cgcaaaaacg
     3121 ctacttggct tatctctcct tctgcacctg taactgacac ttgtggcaga tacccttgga
     3181 acagccaaat gaaaaggccg gcaaaagtag cgataatgat tggattgacg aacatcgctt
     3241 tgatattttt agcttccatc ttcaagccac tcatttttat gtatccataa gaatacaaaa
     3301 agattcggta gccaatattg aaaattgagg aatacatcac gccgacagga ccataaatcg
     3361 cactgacgat aggcgttccg aaaaaggtcg tagaaccaaa gatcgtcagg acacgcaacg
     3421 ttgtttgctt atccccttta tagcttaaga aaaaaggttt tgaaactaga ataagaataa
     3481 tataaataac taatccccaa atcaagacat tgattccttg catcagtaat tctgtattga
     3541 tatcttgcat gaaagctcga aatgccagtg ctggcaaagc cactgttaag acgactttcg
     3601 atagaatttt tccaacagat tcagaaaaaa tccccttttt tctacaaaca aaccctaata
     3661 atataataaa aactgttgaa gtgattgcac tgacaatcga catatctgtc aaagtcgatc
     3721 gaaaaatgtc aaataagttc atctttctcc tcctaattat gttcatttat tcacaataaa
     3781 aaaagctttt attttgctaa tacgtattaa catttttaat tttatcttta cattgttact
     3841 tattgaacaa taggaaatta aagaaacttt tgaaacttat gtaaagtgat ttttactaga
     3901 acacaaaaaa accttaaaac caaacttaag aagtttagtt ttaaggttaa cttaaattat
     3961 attatgcgtg gaaacgttcc acgccatcta gataagttgc ttccaatgtc atatcagggt
     4021 tcaatacgat aaagtctgca tcgcgtcctt gagcgatttt tccacatgta tcatcgattt
     4081 tacagctgat tgctggaatc agactagcca tcatgactgc ttgttctggt gttgctaatc
     4141 cccagtctac aacattttta attgcttctt tcaatttcaa tatacttcca gctaagttgc
     4201 cagattctag acgtgctgtt ccttctttta ctactactgg aaattcgcct aaattgtaat
     4261 ttccatcagg catcccaccg gccatcatac agtctgtaat taatgcgaca tgatcatgtc
     4321 ctgctttttc cattaggatt cccgcagcat ttggatgtac atgatggcca tcacaaatca
     4381 attcagaaaa gacatgttct aaagataata acgcacccac catacctggt tcgcgatgat
     4441 tcaatccacg cataccattg aaagcatgga caaatacact tgcgcctgct tcaacagctt
     4501 tttgcgcttc ttctagtgta gcattactgt gtcctagaga aacaactact ccttcttcag
     4561 tcacttgttt gacaaactct tctacacctt gacgttccgg tgctaaagca atttttttga
     4621 ttaaaccacc tgaagcttct tgccattcat ggaaagtatc caaatcagga tccccgaaat
     4681 aacttgggtt ttgtgcccct ttgtgttctt ctgtgaagaa aggaccttca aaatagattc
     4741 ctttgatttt agctcctggt gcttcctgat acattttgcc gattgtctca gctacatctt
     4801 ttaaacgttc tttgcttgac gtcaatgttg ttggtaaaaa tgaagtcact ccacaagata
     4861 acagaccttc agacatcact ttgatgcctt ctgcatcatt atccattaca tcatgattca
     4921 tatagccgtg aatatgtgta tctaccaaac caggagcaat ccatttgccg ctttggtcaa
     4981 tgatgttgac ttctccctca ggtagttctt tggtgtattc accaaaaagt ccatcggtaa
     5041 tttcaagata gcctgcatgt ttgacacctg acgctaaaaa gaatttgtca gcatagataa
     5101 aagttctcat tctattctct ccctttgttt ttctacctct aaagtactct tatttagaga
     5161 agaagtcaag aatggtctac accatttaca aattttttga aatttgtttc acagcttgtg
     5221 ctaagccttc atagaatgct gctctttctg gttcttcttt acgcttgtat attaagcttt
     5281 taagcacaaa taaatcattg aggtaatagt agctattctt ctcttgagcc caatagattc
     5341 cttgattcac ctgttctaac gcttcgtcga tttcattttg atggaataag aaatttccat
     5401 aattataaaa aatttgcgaa aattcacttt ttgatcgttc atcagctgat tgatgcagca
     5461 gttggatgct tcgactcaaa tagtagcgtg cttcagccgt ttttcctgcc acaccatata
     5521 tttttccgat acaactaatc agttgtattt ctgtgtcaga aacatggatt ctttcttttt
     5581 ggctagagta gcttaatgct tctttcaaat aataaaggga ctgagagata ttttgacgca
     5641 acgtgaattc gcaaacaccg agataataat aatagcgttg aaagtcagtt gcttgataca
     5701 gattttcaga aatatcagaa gattttagta cttgttctaa cttccgataa tcacgttttt
     5761 ggaaacaact atccaacagt tcaaataatt tatcgtttgt ccgagtagtt tttcgagaga
     5821 gattcatcac atgatcaatc gttaccccta gccgttgaca aatctgcatc atcaccaata
     5881 cattcggcag ttcctcatta ttttcgatcc gacttaatac actttgtgaa caaatatcat
     5941 gcgaaagcat tttttgcgtc attttttgtt cttttcgaat ctctttaatg atcgttccaa
     6001 aggactcgat taacgtttcc ataagtatcc tccccaaaat atgcgtattc gcattctatt
     6061 cgtattttac tttaggaagt cgcaaacttc aagaaaagac ttgcaactaa ataatatact
     6121 ttagttattt aagaaaactc cactagaaag agagcctcag tgtgtttgtc tgtggccctc
     6181 ctactgaaaa atttggtttt taaaatcatt tttggcgata ggttgctagg aattctctca
     6241 ttttcttagc tgtttgtttc aaatcatcca ttttaggaag ataaacgatt cgaaaatgat
     6301 ctggctggtt ccaattaaat ccaccaccat gtacaagtag aatatgatgt tcatgtaaaa
     6361 agtccaaaac gaatttttca tcatccaaaa tattgaatcg ctttgtatca atttttggga
     6421 atatataaaa agcagctttt ggttttacgg ctgaaagacc aggaatatca ttgattgcat
     6481 tatagatata ttctctttgt tcatagatcc ttccgcctgg caggagcagc tcatcgacac
     6541 tttgataacc gcctagcgcg gtttggataa tttgctgaga caatacattg gaacacaaac
     6601 gcatggagga caacatattc agcccttcta tatagccttt gacgtgtgac ttatctccac
     6661 ttaggaccat ccagcctact cgaaatcctg ctacacgatg agatttggat agaccgttca
     6721 gcgtaacaac gaacaaatca ggcgctaatg tagcaatcgg aatatgttct agaccatcca
     6781 tcactaagcg atcatagatt tcgtcggaat aaataatcag gttattttgt cgagctactt
     6841 caacgatttc ttctaataat tcttttggat aaagtgcccc agtaggatta ttaggattaa
     6901 tgataacaat agcttttgtc cgactcgtta tttttgattt gatatcatca atatcaggat
     6961 accattctgc ctcttcatcg caaatataat gaacaggtgt gcctccagct aatgcaatag
     7021 aagccgtcca caacggatag tccggcattg gaaccagtac ttcatctcca ttgtccagca
     7081 acccctgcat acacatcgtg atcaattcgc tcactccgtt gccggtgtag atatcattga
     7141 tcgtcacatt cggaaaatgt ttcagttggc aatattgctc aattgctttt ctagcagaaa
     7201 agatcccctt ggaatctgag tagccttctg agtcacgggc attcatgatc aaatcacgaa
     7261 tgacttcgtt tggtgcttca aatccaaaag gtgcgggatt tcccgtgttc aattttaaaa
     7321 tactaatgcc ctcttcttgc atccgctccg cttcttctag aacgggaccg cgaacatcgt
     7381 aactgacacc ttctaatttg ttggattttt caaattctct catcacttag ccaatccctc
     7441 atttcaaaaa gttagaactt tccaaatttt ttgttattta ctatttcaat atcatactcc
     7501 atcctagaaa attttgccac ttatttagga aaaacgtaag tattaaaaaa acacaaacaa
     7561 gcgttgagaa cttaattgtc atgatagtca ttccatagat tcgctgccca atccattgca
     7621 attccacaaa tgattcctag cataaaacca agcaaaaaac gtccgaatgc aagtccaatc
     7681 aatacaccta agatcattcc cagtcctgaa aattctccgt catatttctc catcttaata
     7741 agtcctccca tttttgagct tgacaactat gtaaaacctc gctatcatta gtgtgttatt
     7801 attacgcaga tgtgttggaa ttggcagaca agcatgattg agggtcatgt gagcgttagc
     7861 tcgtgtgggt tcgagtccca tcatctgcac tacaactcgt gaagttaatt ttgcttcacg
     7921 agtttttttg tttgaacgtt tctttatgcc attatataca tatcactgta tcctttacaa
     7981 aaagaacgta caatcatgta aacagcctga ggtgagtata tgaaggtcgg aaacaagcta
     8041 ctcgtagaaa cttgcaagtg ttaaaacaat taatcgaaaa acgagtaaaa tccctcacct
     8101 aatactaaag cgagggattt tttgtatatt atctgtttat caccgttatt ataaaggtac
     8161 aaataccaag cacggttaat ccaaattgca taaattcatt tttttgtaca ctcatgcttt
     8221 tcattagctc tttaactgcc attagtacta agatggcgat aacaacataa agtatatagt
     8281 acgtccgcat tccggaagcc catactgtgg gagaaaatcc cataattaac cttgaacaaa
     8341 atcccattat catcaaaata actaaaaaaa tagaagtttt cttattgtca aaacttaaat
     8401 aaattccaac aattaaacat agtaataatg ccaagactac taaagtagct atccaagtac
     8461 ctggataaaa aagtgacagt ttcgttccta actctgtaaa gagattattc aggttactac
     8521 tactccaaat cattgccctt ttatttccat tgacgtaagt aaagctttga cccattgtat
     8581 taccaaaata aataatcagg tttaagaaaa acggtaatgc agtcaatata cgcacataat
     8641 agttttgaca tttcctatat gttagaagga aaattaagaa aaatagcaat aaaaataaga
     8701 tattcatgtc taaaaaaaac ggctttccaa atgatgaaat acctaaatct aacttattaa
     8761 gaaagttgaa atttttatat tcaggaaacc atttattaat ttcatgtgca gcacgtaatg
     8821 cattccctgg agttgtcaaa gagaaaatta attctgctaa gcttattatt gaaaatactg
     8881 ataacttata attatatcgt ccattgacaa tcaaatataa actaactata cttgttagaa
     8941 caaaaaaaca ggcgttgact tgttcttgat tagttaaaaa tatcaataaa ggaatactta
     9001 cccaatatat ttttctattt atttcttctc ctcttaagag ttgaaaaaac ggataaaaag
     9061 ttagtaaact aaatgctact ggccactgat agttcaaggt ggttgctctc caaccggttt
     9121 cccccattac tgtcaatggt attaaacagt atatagagca aaccaataat aaatttttaa
     9181 catttatttt ctcaaaaaca tacttgcaaa gtaatactac aattcctaac ataatcgtag
     9241 aatttaataa tttccatagc ataaagtgtt tagaaaagaa cattaagaat agttctataa
     9301 taattcttga actccatgtc ttataatgtt ctatcgaagc acccaaatag ccaagttcat
     9361 ctaatctact tgcataaact acgtcatccg ctctcatagt cggcatatac atattaaaga
     9421 gtaaaaagat accccaaatc cctattagta acaagatcag ttctttgtta ttcgttattt
     9481 ttctattatg tttgttcatt tttatactcc ttcttatatt tcctaataga gtataacata
     9541 gctttaagca ttacttaaag ataacttaaa agttatcttt taaatactta attgttaaat
     9601 accttatttg atgcaaaaga aatctacgat agtcaacaaa aaaattttca ttatatccag
     9661 aaaaattttt cttagttaag gacctatgga ttgcagtaat gcagaattcg tcgaataaac
     9721 tgccaaagac atacaatcat attgctttta aaattgacga acaagatata gattcgtttg
     9781 tctcaaaaat ccaaatgtta ggtttaactg tcgaaccagg acgttctcgt gtgaaaggcg
     9841 aggcttcttc aatttacttt tacgactatg ataatcacct cttcgagtta cataccggta
     9901 ctctccagga acgtttaaaa tcctacaatt ccactacctg agactagtaa tgactagtct
     9961 ctttttatgg cctctgatcc ccccattgag cgaatactaa tagggcttcg aattgatctg
    10021 ttcaatcttt aagcaatatc cctcaaatga gtttggaaag tttagtgatt catcacaaat
    10081 acttaaatac atgtagttat atatagattg aggttcttgt gagcgtatgc tcgtgtgggt
    10141 tcaaatccca tcgtctgcat ccatctttcc aaaacaagcg gctaggacat ttttgtctca
    10201 gccgcttgtt ttctagttta ttctttcatc ttgtcggacc ttttttcttg ggtatagact
    10261 tttctcatat tctaccacaa ttttgggttt ccctaacaca tgataggatc tcacaacaaa
    10321 ttgctttcta ttattataat aacctgtaac gactaacttc atcccttcgt ctacttcagc
    10381 taggaagttc aaactatggg aagcaatcaa acaattgacc ccatttaaac tgaaacggat
    10441 caacggtctg gcactaactt gcaaagtacg gatcttatct actgttccat attttttctc
    10501 cataaccttc acctcaatac cattatacga acatacattc cctttgtaaa gaagaaactc
    10561 tgttttatct ttttgacttt ttgtctatta cctttcttaa aatcatattg gcacttcctg
    10621 cgaagatgaa ggacatgtac taaaaaaaag aggcagcagt atagattact gttgcttctt
    10681 tactaattta ggtttacttg ttaagaaaga ttatttacgc aaatgttctt caacaatttg
    10741 ctgagattta tcagctgtat cttccacttc atcttttgct ttttctaact tttgaatata
    10801 ttgtttcaag cttgagtttt cagcagttgc ttcgtctaat ttcttttgtg tatctgttaa
    10861 ctgagtttct acatttgttt tttcttgtgt cagtgttgcg atttgttgat tcaaaccatt
    10921 tacagtattt tgtaattctg aaagttttgc ttgtgcttct tgtagtcttg ctgtccaatc
    10981 tgcattcaga tgattgattt catcattttt ttggttgatc tcatctagtt tgacattgat
    11041 ttcattttgt ttgctttgta gttgtgcatc cttgctgact aattgacttt ctaagttttg
    11101 tttttctgct gccgattgat ttttttggtt ttctaattca tttacttttg tttggtattc
    11161 ttggacatct gcctttgaat cttgcaatgt ctgttctttt tgtgctagtc gagcggtaag
    11221 atctgtaagc gcttgctctt tatctgtcaa tgtatgatca agctgtgtca gattctcatt
    11281 gattgtatca atatctgtct ctccacccca caaaataatt tcatttgcgt accgcgtgcc
    11341 tgtgtagcca attagacata gaaatgcaac gacaatccca ccagtaataa atttcttttt
    11401 catttgcata gctccttaat ccaatacttt tacatgctaa aagtaagtga tctcaaacac
    11461 aatgtcaagc gtttttaaat tacatgtgta attttaatat aaatactatc aaatactcat
    11521 ttaatcctcc ttttttatct atttaaaaaa tcttttttat agagaaaaat gcaggttaat
    11581 actttcttaa ataaatattt atggtgaaca aaaacaaaaa agcaagtttt attaaacgta
    11641 acccttttca tttcatagat tactgtattt attacaaaaa acgcggattt gtcataaatt
    11701 gtcatgcttc ttttacctat gaaatatttt cacgtgttaa agtattcctc gttgaacaca
    11761 acaaataaaa ctttttaacg aataaaggag atttttcatg acatcactta aaactttact
    11821 ttttggaaca actttggctg ccggcgctgc attcttcatg ggaacgactg ctcatgcgga
    11881 cgaagcttat accgttcaat caggtgatac tctatcaacg atttctcaaa aatacgttgg
    11941 tgataactcc ttgatcaatg ccattgcaga atcaaattca atttctgata ttaacctgat
    12001 ttactcagga caacaattaa caatcccaac agaaggctct gcacaagcag ctgctgaacc
    12061 tcaagcagcc gttcaagaag caccagtcca agctgaacaa cctgttgtac aagaaacggt
    12121 tcaaacggaa aaacaagcag cgcctgttgc tgaaacacaa cctgctcctg ctgtaacaga
    12181 aactgctgct actccagcaa gcacaagctc tgcaaaagaa tggatcgcac aaaaagaatc
    12241 aagcggttct tacacagcaa cgaacggacg ttacatcgga cgttaccaat tagattcttc
    12301 ttacttaaat ggtgactact ctgctgcaaa ccaagaaaga gtagcagaac aatatgttac
    12361 ttcacgttat ggttcttggg aagcggcgaa agcattctgg gaagcaaacg gttggtacta
    12421 agattcattt attaatcaaa cagtttactt ttatacaaag aaagccaaaa agaggtgcga
    12481 cactagttgt gtcacatctc ttttttattt acctaatcta atgaaacgtt ccaagttttg
    12541 ataagtattt tttttaactt cttcaactga tagctctttg atctcagcga ttgtctttaa
    12601 ctgttcttca agcagtgaga cataatcttt ttctgaaaaa atccccccta aagcccactc
    12661 tgctgcatgt agtccatccg tttcaataag taagtggtcg attgggactg ctttagctag
    12721 ttgccagact gctggattct ttttcaaatc aagaccaatg ctgaaataac agcctaaatc
    12781 aatatagtct tgttgatagt ccaaagaaga ataccaatgg atcaaatagt gattagggta
    12841 agcttggata taatttaaga tctctttttc gcatcctttt gtgtgcagta caacaggttt
    12901 ctcatttatg gctgcaaaag caagctgtcg ctcaaatact tttttttgtg tagtcatcgg
    12961 tacattcgtc caaatattat ctaaccctat ttcaccgatg atccttgctt ttttcaaaaa
    13021 aggctcaact tgttcaaacg tatagctatc tgctttccaa ggatggatac cgtaacttaa
    13081 ggcttgagtc ttacttgctg caagctgtct gttttccttc cattcttctg gcgaatcaca
    13141 attgatgatc gtcaaaatcg tgtgttcctg ctgaaggagt gctaaactct gactcccatc
    13201 taaatgacaa tgagcatcac ttaacatcta attcacctgc caataactga taaatcgttt
    13261 gcttcaactc tgattgctgc agtagccatt cacgtgaccg tcgtttttca ggcacttgaa
    13321 tctcagcaat cacatgtgcc ggacgggcgg aaagcacaag aatcctgtct gataaataaa
    13381 ttgcttcttc gatatcatgc gtcacaagta aagtagtccg ctttgctttt ttaggcagct
    13441 tcaacatcca gtcttgcatc tgccctctgg tcatagcatc taacgctcca aatggttcat
    13501 caagaagcaa gtaagaagaa ggattcatga ttgttcgtaa caaggccgtt cgctgcttca
    13561 tcccgccaga taattgttcc ggcaaaaaat gttcatagcc gtttaaccca aagatatcca
    13621 gataatccct tacttgttct tcattgattg ttagatgatt gattttttga tataacgtaa
    13681 cgttttgata gacgctcagc caaggaagta acatatcttc ttgcggcata taactaaatg
    13741 ctgtatggag accagttact ttttgatcgt ctactttgat cgttccagaa gtcataggct
    13801 ggactcctgt taaagttttc agcaatgttg acttaccggc tccgcttggc gcgacaatcg
    13861 atgtaaaggt atctttaggg atagtgaaat ggatgtcttc taatattttc aggttttcaa
    13921 tgactacatt caagtggctg atttggattg acatatctat cactcctatt ttataaactg
    13981 attagtgaat agttcttgcg aaggtacatc ttgcttgatc aatccgtttt cctgcataaa
    14041 agctgtataa ttgcgccaaa ctttttcttc catcaatccc caattctcag gatcatccgt
    14101 ataatttttt gacaagaatg cttgtgattc ttctaaaaac gtccgatcat tttcttttgc
    14161 atatttttgc aacagtttag cactgtcatt tggatcctta atagcttcct gatagccttt
    14221 tttcgtggca tccataaaag actgtaccaa ttctggatca cttttgatca gttgatcatt
    14281 cgtaataatt accggagtat agtaatctaa ccgttcatct aattctttca atgggatata
    14341 gtttagttca atacccgctt cttttgcttt gatcatatcc caaccttcaa agtaccattg
    14401 gatatctgag gattttccta agctttctgg cccttctcca ttgcttccta ccattgtcag
    14461 ttttgaaaag tccccgccgt ctttttccat cacagctttt aagacagctt cttcacttgg
    14521 actttgccaa cctgcatatg tttttccttc aaaatctttt ggtgaatgga tgttactgtc
    14581 tgatagggtg gcgaatccag aagtattatg tttgatcact gtagcaattg ctttgactgg
    14641 tatattctgt ccatctgcgt gagcataagt gacatcttct tgataactta caccaaattg
    14701 tgctttgtcc gcacctacta gaccaatact tgtcgaatta tctcctggtt cgatgatttg
    14761 gacatttaaa ccagcctctt tataatagcc tttttctttt gctaggtaga tgccagtatg
    14821 attagtattc ggtacataat ccaaaacaag tgtcactttt ttcaaatcct ttatttcttg
    14881 tttgctactt gtttcttttg cagtgccaca agcggataaa agcaacagta gcggtaataa
    14941 aagccataat tttttcattt gatccccttc tcttaaaaaa tataaagcat acgataatac
    15001 ccttttttca ataatgaaca agcgccattc agcaataaac tcaatagaat gacacaaatg
    15061 atcgcagcaa aaactttatc gatctggtag ctgtttttta cgcgtatcat gtaataccct
    15121 aagcctgctt gagcgcttag ccattctcca acgactgctc cgctgacaca ataagtagca
    15181 gccactttta aaccggaaaa gaagccggat aaacccgctg gtattttata tattcgataa
    15241 atcttccctg tatctgcccc catcgttttc aaaaagatga tttgctcttt tgatactttc
    15301 ctcaatgtat ccgaaaaaga aactacgatc ggaaaatagc ttatcaagac aacaaggatc
    15361 acttttggaa gcataccgaa accaaaccat agggtaagta atggccctaa caccatgatt
    15421 ggcaacattt gtgaaatcac tagtaacgga taaaaagaca tttctaaaaa cttccaataa
    15481 tccattgcca aggcagtaat aaaagaaaga acgctagcta ttaccaaacc aagcactgct
    15541 tcatttaatg tcgtgacaga atgggaaagt aatacttgtg catctgccgc taatgcagaa
    15601 aatacagcag caggtccggg taaaataaag gcaggtattt tccaaactat gacaacaatc
    15661 tgccaaataa ttaaaatgaa tgccatactg actgctggat aaagattttt cttcattttg
    15721 ttacctcctt tcttctaata tgaataacaa aaaacgcttg aaatgggcac gacatagtaa
    15781 cccgcttcaa gcgtctgcta tgtttctccc ttcaccagta ttaactgaca ggttcaatgg
    15841 gtctaagttc acaacttact ctcatgtcgt ctacgacatc cccagaaaca ggtattcatt
    15901 ttttaaagtg tagcacaaga aggaaaaagc aacaagcgct ttagttatca tgctgattat
    15961 ttcaataaat aggagagatc acttgccaat gtcggatacc ccatgatata gctatccaac
    16021 tcttttttag tcaatttttg attgatgacg agtgtcaaat catcgatcaa ttcgtctgct
    16081 tgctcgctaa tcactgcagc accaatcaaa taattttgct ggtcaaaggt caattcagcc
    16141 ttagcaactg gttcattgac acgatgatag gtgaaccaac tggtaagatc catctctacc
    16201 acttgatctg acgaagaggc gtgacttttt gtaactccta cttcagcaag tttaggactg
    16261 gcgtagacga tcgtaggtat aatcggatat ttgataggtt cgcttgttgc atccgttatc
    16321 cgcttagcta cataatttcc ttcaaaagtg gcgactggtg tcaattttgg ggttttcctt
    16381 gaaacaatat ctccacacgc aaaaattttt ggattgctcg tttgcagata atcattgact
    16441 gcaattccgt gtttatcaaa gacgatattc gcttgctcaa gtgctaaaga ttctgtattc
    16501 ggctgtctcc cagtcgcaca aaaaatcata tctgctacta attccgtctc ttttccgact
    16561 aaacgatagc gagtaccttc tgaaatgatc ttttgggttt caacaccaaa agcaaactga
    16621 atgccagatg cttccatttg atggacggct tcctctacca aagacgcctc aaattctttt
    16681 aatggccgtt gattatgatg gacaatggtt actttgctcc ctgcagcatt ggcaatcgta
    16741 gctagctcaa aagcaatata accaccaccg ataaaaatga tttctttagg cagtacaggc
    16801 aatgataaaa agtctgcact agttttcaag tattcctttc cctccactgg taagatcgta
    16861 ggtctttgcc cggttgccaa aactaagtaa tccgcatgga agacttcttc gttgacttca
    16921 atagatgaat catccaaaaa acgagcggtt cctgaaagat gatcgatttc agcttctgct
    16981 aattgttttt ttcggctttc tgggactgga tccgtaaatg ttcgtttgaa tttttgaagt
    17041 tcttcccagt tggcagttgg gatctcgtta aaaccttttc cactcaattg ttttacccga
    17101 ttacgtgcct ctactgcact taacaagact tttttaggat cacatccacg gttaggacaa
    17161 gtcccgcccc atagatcttc ttcgaccact agtactgttt tacctgcttc tttcaatcca
    17221 taagcagcac ttaagccgct cacacctgaa ccgatgatga ttgcatcata tgttttcatt
    17281 tccttcctcc tctcgactta aaaaagcaaa gatttcttta cttgtcagcg acccaatact
    17341 tctttgcttt tctactatca caatgcttgg atacaccgaa agaagcgaat atacttcttt
    17401 cataggcgta tcaaagcaaa cttgttgacg agcattttct ggtatttcat ttaagaaata
    17461 tccattgacg actaaatctt ctactcgcca ctctttcgtc tgtttcggtt catcaaagaa
    17521 atgccgaacg tagtcgtttt taggattcat caataactgc tctggtgtgt cacattggat
    17581 aatttctcca tccttcataa atgcaatacg atcacctagc ttgattgctt cttccatatt
    17641 gtgcgtaacg aaaacgatgg tcgtgcccaa ttcttcgtgt aaggacaaaa ccaattcttg
    17701 taatgagttt cttgaaatcg gatctaatgc gctaaatggc tcgtccatca aaatcacatc
    17761 tgggcttgca gcaatagctc ggatgatccc gatccgttgc tgttctcctc cagacaactc
    17821 tctaggcata cgatctcgat aaatatcagg agataagcca actttttgaa gaagttcatc
    17881 tacacggtct gctcttttct gcttttccca acctaacatt tcagggatga cttcaatatt
    17941 ttgcttgact gtcatggtcg ggaacaaagc gatttgctgc agcacatatc ccatattcca
    18001 ccgcattttc tgcagattat attcatttaa cgattttcct ttgaagtaga tgtctcctgc
    18061 tgatggttta gataatccat tgatcatttt caaagtagtt gtttttccac taccggaagg
    18121 accaaccaaa acaaagattt ctcctttttt gattgccaga tttaggtcat cgatgacata
    18181 ctttccatca tacttttttt gcacatgttt gaactcgatt aatttagtca ctgatcttcc
    18241 tcctttacta atccttcatt tttcaggaag attcgtgcta cttctgtcgg ctttttcttt
    18301 tcgacattga cttggtaatt catatcaatc atttgatcct ccgttatttt tccgcttaat
    18361 ttattcagtg ctttaacgat ttcaggatgt tctttagcaa aatctttttt catcagcggt
    18421 gcgccttgat aagctgggaa taaccctcga tcatcttcta gagtcactag atcgtactcc
    18481 ttgagctggc tatctgtgga ataggcatcc acaatattga catctccatt attgattgct
    18541 tggtaacgca aacttggctc catgctttgt acacttgaaa aattcaagcc atatgcttct
    18601 tggattcctt tatatccatc tgaacgatca ataaattcca gtgtgaatcc cgctttgatc
    18661 tgatcagaga tagctgccaa atcggaaatc tttttaagtt gatgttcgtt cgcaaaagat
    18721 ctcttgactg ccaaggcata cgtattttgg taagccattg gttttaataa tgtcatatcg
    18781 aattgcttat tcaataattc ttctgcttcg tcatatgttt tttcttttgt caaatcagca
    18841 cttttcattg cttcaggtac cttcaccaag ctttctagga cagtacctgt gaactcagga
    18901 tagatatcga tttgaccttg atccagtgca ttgaacaaaa aactcgtctt gccaaaattc
    18961 ggttttaatt caacttctgt ttttgtgttg tcttcaatca attctttata catttggatc
    19021 aggatttcag gttccgctcc taattttcca gcaatcgtga tggtttcatt tttaaacaag
    19081 gcagactgat ataaagaaac gccgccaaat aaacacacca ctgctgctaa agtaatgatg
    19141 gaatatcgag gtttcttatg ctgcaaccag cgaatcagtc cgctgaatat aattgcaagc
    19201 aaggcagaac tgatcgcacc aatcaatgtc aaaacaggtg tatttcggtc aatacctaac
    19261 aaaataaatg tccctaatcc acctgctccg atcaatgctg ccaaagtagc cgtaccgatg
    19321 attaaaacta aggcagttcg gatacctgag ataatgacag gtaatgcgat gggaagctct
    19381 actttcagca agcgtctcat tctggacata ccaaatgcta ccgctgcttc ttcaatagac
    19441 gggtcaattt cagctagacc aatatacgtg ttttggaaaa tcggaagcaa agcgtaaata
    19501 acaagtgcaa tcaatgctgg aacagttccg atccctacaa atgggataag taaacctaat
    19561 aaggcaagtg aaggaatcgt ttggagcaca cccgtgatct gcaataagac ttctgccatt
    19621 tttttatgat tggctgccca aatcgctaaa ggaatagcaa tcagtgctgc aatcagcaaa
    19681 gaaatgagag aaatggacag atgttgaaat gcagcttcca gcaactcgcc ttttctttca
    19741 cttaatgttt gtataaaatt attcatgcga ccttcctttc tgttattctt taagtataaa
    19801 catttctttc ctttttccca actaacttgt cttgccttaa ccttacagca cgaacaaaaa
    19861 aaagaaaaaa atacctctaa atatgctgac aaaaaaagaa tccaagcaac aaagtcggat
    19921 caactttttg gttctcgttt tttcactacg aataccataa aaatctatac gaaattgtca
    19981 cttggaatca gctatatctt ttttacacac acaactttta ttttaataaa aagaaacagc
    20041 caataaagta gaaatctcgt gggggagaac acctttattg gcctgttcaa cgaatattat
    20101 actgtatcat gacacttttt tgccaaaaat gtaaaaaatg gttgttttga catcataaac
    20161 gtcttgtttt cttattttta cttatatcac cactttattt tctatctctt tcactccata
    20221 aagaggaatg agttcttctt ttgactgata ataaacagct ttctcacggt tcttcatcaa
    20281 aaaaagaaag atgtctttca tagcaagtgg tgctttgccg tgttcaaata aaaagataaa
    20341 ttgatttgct ttatttatct ggaccattgt cacaggcacc agtttttgtt cttgagccaa
    20401 aaaaactcct tgatactctg tttttttggg tgattttgta agaattgtca ataagtctgc
    20461 tgctcgcatt tttccactcc ttatttgata aataaaaaga aacagccaaa aataattttt
    20521 tttcattttc ctcttgaagt aagagtataa gtcagtataa taaagaagtt gctgaaaatt
    20581 ttcaaaagaa aagaggctag aaatgaaaaa attaaattta tttacagaat tgtgcggaat
    20641 cattttaggc gcaggaatct acggattagc tgtgacagga atcaatattc catctaagct
    20701 ggcagatggt ggggttactg gtattgcttt gcttttgcat aatctttttg gattcgctcc
    20761 ttctgtcact tcactgcttt ttaatttacc tttattgctt atctcattgt ttctttttgg
    20821 aaaacacgct tttctccgga cgatcgtcgg aacatttgcg ctagttttct ttttacattt
    20881 atgggagagc cttcatatac attttgctgt agatagtcta ctgatcaata gtttgatgac
    20941 aggtattctt tctggcattg gttgcggtct cgtctttcga ttcggcggaa gcacaggcgg
    21001 aacagatatc atttatcaag ccattgaaaa atattttcat attaagattg gcaaaagtct
    21061 tttcttgatt acttttggta ttttagttgt ctccttgctt tatttggatc tcacacattt
    21121 tctgtataca ttacttagct gttctattct atcttataca ttgaataaag tgaagtatct
    21181 ggaaattgga tctgttttgc cccaattgga agcaaaacag actacagaaa catttgaacc
    21241 acttgatcct cttgaacagt tagagcactg attcttattt ttcactactt tatttaattt
    21301 acagaaatat cattatgtaa actaaaatga tattattcag taaaatcttc tttataaaaa
    21361 aa
//
LOCUS       DAJSBL010000044        21283 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.044, whole genome shotgun sequence.
ACCESSION   DAJSBL010000044 DAJSBL010000000
VERSION     DAJSBL010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21283
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.044"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..152
                     /locus_tag="ONT85_001959"
     CDS             <1..152
                     /locus_tag="ONT85_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR2378927.1"
                     /translation="NFAKNQGYNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIY
                     MKKTL"
     gene            complement(230..763)
                     /locus_tag="ONT85_001960"
     CDS             complement(230..763)
                     /locus_tag="ONT85_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HCR2378928.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(857..2035)
                     /gene="mutY"
                     /locus_tag="ONT85_001961"
     CDS             complement(857..2035)
                     /gene="mutY"
                     /locus_tag="ONT85_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HCR2378929.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(2028..2825)
                     /gene="recX"
                     /locus_tag="ONT85_001962"
     CDS             complement(2028..2825)
                     /gene="recX"
                     /locus_tag="ONT85_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HCR2378930.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            2932..4314
                     /gene="rlmD"
                     /locus_tag="ONT85_001963"
     CDS             2932..4314
                     /gene="rlmD"
                     /locus_tag="ONT85_001963"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HCR2378931.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(4488..5066)
                     /locus_tag="ONT85_001964"
     CDS             complement(4488..5066)
                     /locus_tag="ONT85_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic polysaccharide monooxygenase"
                     /protein_id="HCR2378932.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            5388..5858
                     /locus_tag="ONT85_001965"
     CDS             5388..5858
                     /locus_tag="ONT85_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HCR2378933.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLTMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(5966..7192)
                     /locus_tag="ONT85_001966"
     CDS             complement(5966..7192)
                     /locus_tag="ONT85_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HCR2378934.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(7946..8146)
                     /locus_tag="ONT85_001967"
     CDS             complement(7946..8146)
                     /locus_tag="ONT85_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HCR2378935.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            complement(8280..8888)
                     /locus_tag="ONT85_001968"
     CDS             complement(8280..8888)
                     /locus_tag="ONT85_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HCR2378936.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            complement(8973..9443)
                     /locus_tag="ONT85_001969"
     CDS             complement(8973..9443)
                     /locus_tag="ONT85_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HCR2378937.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            complement(9491..10405)
                     /locus_tag="ONT85_001970"
     CDS             complement(9491..10405)
                     /locus_tag="ONT85_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HCR2378938.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            10551..11354
                     /locus_tag="ONT85_001971"
     CDS             10551..11354
                     /locus_tag="ONT85_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HCR2378939.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            11532..12479
                     /locus_tag="ONT85_001972"
     CDS             11532..12479
                     /locus_tag="ONT85_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HCR2378940.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(12547..12840)
                     /locus_tag="ONT85_001973"
     CDS             complement(12547..12840)
                     /locus_tag="ONT85_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis protein"
                     /protein_id="HCR2378941.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEMDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(12868..13533)
                     /locus_tag="ONT85_001974"
     CDS             complement(12868..13533)
                     /locus_tag="ONT85_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HCR2378942.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(13673..14305)
                     /locus_tag="ONT85_001975"
     CDS             complement(13673..14305)
                     /locus_tag="ONT85_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR2378943.1"
                     /translation="MIKVLLVDDHEMVRLGVSSYLSIQEDIEVIGEAENGRQGYEKAM
                     TLRPDVILMDLVMEEMDGIESTKAILKDWPEAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNEPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(14312..15379)
                     /locus_tag="ONT85_001976"
     CDS             complement(14312..15379)
                     /locus_tag="ONT85_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HCR2378944.1"
                     /translation="MMGKISRAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLLAISLGVGLIVFLLLSLIQKTKYGKIEEKLRALSSGNYESKLLLLP
                     IPSASDDLYIKDIDKEITKIKEKMIEVSSELQIVTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLSSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            complement(15376..16107)
                     /locus_tag="ONT85_001977"
     CDS             complement(15376..16107)
                     /locus_tag="ONT85_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HCR2378945.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKHPRTKFQNFQLILGSLVIFFSLVNSPALWMMAVFAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMIQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAVISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKIYSS
                     DYDENPRRLKIITNTLIGDVEVIRI"
     gene            complement(16276..16755)
                     /gene="greA"
                     /locus_tag="ONT85_001978"
     CDS             complement(16276..16755)
                     /gene="greA"
                     /locus_tag="ONT85_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HCR2378946.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGE
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(16891..18066)
                     /gene="mltG"
                     /locus_tag="ONT85_001979"
     CDS             complement(16891..18066)
                     /gene="mltG"
                     /locus_tag="ONT85_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HCR2378947.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELHQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVEKYVNNS"
     gene            18270..19439
                     /locus_tag="ONT85_001980"
     CDS             18270..19439
                     /locus_tag="ONT85_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HCR2378948.1"
                     /translation="MKKGKHAKKRKYSFLMIVVVLFSLSAFSTGTILGWQFYNNEHSQ
                     IVAHAQSAQKKLILTTNEQQLVEKIDQLLRKNDYIGSIYVKKNNRIIMEKGYGYANKL
                     TQQPNDPSLYYQIGSVQKALTTLLILKQVERGHLSLDTKLAQFYPQIAGSQQITIRDL
                     LYMRSGLHRTASPTIPMTDEEVVQFAIQHLKFTDYQTSHYDSLNFTILTGILIKLTNQ
                     PYEKLLTDDIIEPLGLQHTKFYDQVKNSSNHALSYQMSKEDDYFKPLTESETEIHNEL
                     GTGNISMSVYDLNTLFTQVLSGKLVSKDLLFSLWRENANGHPYSGGVYSGDNYILSQG
                     NIDSFDTSAIFSKDSQDAIIMESNIKADKTIKLPTADLRNQIYDLMEGTTKLANS"
     gene            complement(19706..20620)
                     /locus_tag="ONT85_001981"
     CDS             complement(19706..20620)
                     /locus_tag="ONT85_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2378949.1"
                     /translation="MIEQTLYQDLNRPYVDILQKRAIYLGKEFLIGSIDSELLSFGIG
                     LTLLICLIILTFNFKKLGILSKITYIMGIISILLCTKIFPWFALQNTPLNIIQAPWRF
                     MIFSTLFISFSVSLFISPQLEKFSYTRRRNFILLLILAISILNYSSVVNMVKEMNFQG
                     TYSNEQMENKLEEFENFDYVPIGYKKDQLLLKNHQVTEDGQVIYSSFSPNKDEIIFTF
                     KSSKGKTIVLPVFRYKGLTVTVNSKNATVLKQGGPFVTVKSESGINKVTIRYSYTFFS
                     KISLFISIITLIVLLFYCIKLKIRTSHE"
     gene            complement(20678..21283)
                     /locus_tag="ONT85_001982"
                     /pseudo
     CDS             complement(20678..21283)
                     /locus_tag="ONT85_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177099.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         6561 a         4170 c         3363 g         7189 t
ORIGIN      
        1 aaaactttgc taagaatcaa ggctataata cgattttatt aggggcacag tccacagctg
       61 aaacttttta cgaaaaacta ggatatacag cttacggcga tccttttgaa gatgctggca
      121 tgcctcatat ttatatgaaa aagactttat aaaaatttat ttcgaaaaaa gcaaaaaaca
      181 gcaagctttt gagaaaaatc tctcactggc ttactgttct tttttgtgtt tacttacgcg
      241 acaattgctt gtaacggtta taccaaatat caatatagcc ttccgagaac gggccatctc
      301 cattattgat ccagtcaacc agaatcttca cattttcttt taggatgaag tcaatatcgt
      361 tcgggtaatt catcattttg ctgtgcatct catattcatc gacatccaat agtcgtttct
      421 cgccgtcagg aaataccttg atatccaagt catagtcgat atatttcaag gcctcatcgt
      481 ccagcaaaaa aggtgaagct agattacaat aataggaaac ccccttttct ctgatcattg
      541 ctattacatt gaaccagtat tttttatgaa aataaacaat cgctggttcg cgcgttaccc
      601 atcggcgtcc atcagattca gtgactaatg tgtgatcatt cacgccgatc atagagtgtt
      661 cgcttgtttt taataccatg gtatctcgcc acgttcgatg caatttgccg tcgtgcttgt
      721 aactttggat cgtcacaaac tctccttctt ttggaactcc catagcaacc cttccttaag
      781 cagcattttg tgctttgtgt ttgggtgttc attacaccga catcataatt attatatcaa
      841 taaaaatgag ctttgcctag aaaggattcc tattgtttcg atttttctct aactgatcta
      901 ccagtttcat ttggacctta ggaaaaacga ccgaatcaaa tgcatcaggc ttcaaccact
      961 tcgaagtttt gttctcggta aattcttgtt ctgctccctc ggttgcccgt ccgtaaaaaa
     1021 ggagaacatg ccatttcaga tgactaaaaa tatgagtaac ttctcctaaa tgtcgtgtct
     1081 gccaaacaaa gagttcctgc ttttcaaatg ggagattttg ctcttcttct gctactaggt
     1141 catcaaacag atcgatttct ggctttgttt cccattcttt tttcaatcgc tcatactcct
     1201 cttgtgtcac ttccatcatt ggaaatgtcc acatattcgc cagcaatttt tggctatctc
     1261 tttcttcaaa ataatacgct ccagaatgat tttgaagagc tgcactgata taataaacgt
     1321 cttttggctt tgcttttttt gtttttacag gaaaactcgt ttggattcct cgtttgttag
     1381 ctaaacaaaa ggcttggatc ggacaagtct cacactttgg agaagttggt gtacagatag
     1441 ctgaacctag atccatcatt gcttggttga attcacctgg atgcttttca tcaatgattt
     1501 tccgcatcgc ttcatcaaag attttcctgc tagaagcttt tgcgatgtct gcttcaatgc
     1561 aaaacaatct gctgactact cgcatcacgt tgccgtcaac tgcaggttct ggaagtccaa
     1621 atgcgatact tgcaatcgcg cctgtcgtat atggtcctat tcccttcaat gagctaattt
     1681 cttcaggcgt ttgcggcatt tccccatcaa attctgacat gatctgttta gcagcagctt
     1741 ggatgttccg ggctcttgag taataaccaa ggccctccca tgcttttaaa agcttttctt
     1801 ctggagcatt tgctagctct tcaattgtag gaaaccattc catgaaacga taaaaataat
     1861 cgatcactgt gtctactctg gtttgctgaa gcatgatctc agagatccag atacgataag
     1921 gatcacgatt atagcgccaa ggcaggtttc ttttttcttg ttcataccat tgaatgaact
     1981 gatcttgaaa ttcttttgtt tctttatctg tccatttttc ccattcttta ttcactttcc
     2041 tcttcctttt ccgtaatgaa tcgctggatc atatccaaat caaatccttt ttgatataag
     2101 ctttgcttta cttttagatt tcttttttta ggttcaaacc ttaaatttct tcgccatagt
     2161 ttatctcctt gtttgactaa ataatcatat tctatttctt gatctgcttc ttgcggaaga
     2221 tcagctatcg cttgttggat cacttcttga gaaaagcctt tcgtcatcaa tgtttgttgg
     2281 atttttctca ataattcttt ttggcttttt ccgtgatttt ttccaaacgc tttttcggct
     2341 gtctgatgtg cattttgaac ttggtcttca aatgtatatt ggctgagcgc ttgttcaatg
     2401 atttcaggtt tcaaaccttt ttgctgcatc tgctgcgcta atttctttgg tcctttgtca
     2461 cttaagcgca tattcgtgcg cacataactt tccccataga ccaaatcatc gacaaggtcc
     2521 aattctttta agcgggctac gattttattt cgatcttctt gaaggatttc cttttctttt
     2581 aagtatgttc ggatttcttt ttccgatcgc aactgataac ttaagtaatt cattgctagg
     2641 tgaaaaccga aatcagctcc gctactcttc ttgatctctt ggatagtttc ttcgtctaat
     2701 tcccgtcctt ttaataatcg atagcgtact aatacatctt cagatacttg taaagcttct
     2761 ccttcagata aatctacgcg atagaaaggg cctttgcctt tactgattct cgtaacggtt
     2821 aacataaaca tcctcctcga acatatattc tctatcatac cacagaaaat ccccgtaatc
     2881 aggtattata tgttagaata aaccacagaa aaaagaaaaa gggagatact tatggcagaa
     2941 acaattgtca aaactggaca aacggttcct ttgaaaatca agcgtttagg cataaatggt
     3001 gaaggaatcg gttatttcaa acggttgatc atatttgttc catatgcact accaaaagaa
     3061 gaagtactag tgaaaatcac gaaagcaact cctcgctatg cagaaggaga attaataaaa
     3121 gtcaaaaaaa caagcaaaga tcgcgtagtg gcaccatgtc ctgtttacta tgaatgcggt
     3181 ggctgtcaat tacaacactt agcttatcaa gctcaattgg attttaaaaa ggatctgctt
     3241 ttgcaagcat tggaaaaatt caaaccagca ggctttcgat cttatcaact tttgccgacg
     3301 atcgggatgg atgagccttg gcattataga aacaaagcac aatttcagtt acgaaaaaat
     3361 aagcagactc aaaaaattga agctggttta tacgaagcaa actctcatca gttagtccct
     3421 ataacagact gcttggttca agaacctcga acacaaaaag taatgaatac tgttgttcaa
     3481 ctattgaata aatttgatgc acccatctat gatgaacgga caaacagtgg aatcttccgc
     3541 acaatcatgg ttagaatcgg tgttaaaact ggggagttac aagttgtttt tattactcgc
     3601 agtaaaaaat tcccgcaaaa aaacgcaatg atccgtgaga tcaatgaacg actgcccgaa
     3661 gttgtttcaa tcatgcaaaa tgtccaggca caaaaaacgt ctctcgtgat gggggatgaa
     3721 acgatccact tatggggaaa agaagccatt gaagaacaaa tcaatgaagt gactttcgat
     3781 ctttctcctc gtgccttctt tcaactcaat cctcagcaga ccgaattgct ttatggcgaa
     3841 ggaatcaaag cattagatgt ccagcctaat gaaacgatcg tggatgctta ttgcggagtt
     3901 gggacgatcg gcttaagtgt agcaaaacaa gccaaagaag tacggggaat ggataccgtt
     3961 cctcaagcaa tcgctgatgc aaaaaaaaat gcggaaagat taggatatca caacactcga
     4021 tatgaagtcg ggacagcaga gcaagttctg cctaaatggt tgaatgaagg ttttcagcct
     4081 gatggtatca tcgtagatcc accacgtacg ggtttggata acgcattgat ccaatcatta
     4141 ttgaaatacc ctccaaaaaa acttgtttat atctcctgta atgtttctac acttgccaga
     4201 gatctggtta atttaagtaa aaagttcaag attgaatatt tacagtccgt cgacatgttt
     4261 cctcagactg cacgttgtga agttgtcgtg aagatgacga gactcgctga ctaaatgaaa
     4321 gaggttgtga aaaagtctgc cctgtatcaa gtaataagaa cagccaaaca aaaatagctc
     4381 ttcatgtttt atgaatactg tacttgtgac cgaagagtta acaggaggac actatttatt
     4441 taaaaaaagg gttgtgacat agtttttgtc acagcccttt ttgattatta ttggatattc
     4501 acgtctatca cttgataaaa tgcatttgtc gtatcgctga tattccatac tgctaaaatc
     4561 acattatatc cttttcgatc ctgcggaagt gtgatcgttt ggttgactgt ttttggtgga
     4621 acagttgctt tgtcgtctat ttttgtgatc aattcaaaat cactaaattt caaaggttga
     4681 ttaggattcc aagttggttt ggtaatgtag taatcccaag tacttgtccg atgttgtgct
     4741 gttaaattcc aagtaatatt taatgttcct ggattaacca tgtttttatg ccatctgtta
     4801 gatgtctgtt catctaaagg ctcaaagccg ctgactcctg cactagcaat ttttccatca
     4861 atgaatgtat ttttggtagc ttcgatactt tgcggttccc attgtgctct tccgacattt
     4921 tgatttaagt ttcctgctgc tgatgttcct aggtatgcac ggcttcctgg agaagtaata
     4981 taaccatgcg caaaacctgg aatgctaaaa ccgaacaacc ctaaactaac taacccaata
     5041 aacaataacg tactaatctt tttcatgtag aaacctcctt catttgataa ccacattgta
     5101 aacgctttat tgtattaaca caaacattat gcttttataa ttttatgttg aagtaaaaaa
     5161 ggtgaaaaaa gtaagatgca gattataaca gaagtagacg agaaaagtac aaatagaaaa
     5221 catgagaatc aaatcatatg atctttttca aaaacatcat tcatataaac aaatgttcaa
     5281 ctttttaata acaacaatta tcaatttttt tcttacttca caaactaaat acaggtcttg
     5341 ctgtgagcct tcatggtaaa ctaatattat agaaaggagt tagtaaaatg aagatgataa
     5401 aagatattcg tttttgggtt tgtgttatag gaattgtcat attaggcttt ttatcagggt
     5461 tgcttagcgg aaatcccgga aaatattact attctttgca acttccacca tttgcaccac
     5521 ctagctggat atttgggccg atgtggacct tactgtatat attgatgggt atttctcttt
     5581 atttgttact taatcacaac aacaaaaaac agcgaaacaa tcttgtcggt ctttttgtga
     5641 ttcagttcat ttttaatttt atatggtctg ctctattttt taatttaaga aatattttca
     5701 ttgcagcaat cgatattaca cttttagtta ttttcttgtc agtgcttatg tatcagttgt
     5761 ggctgcatca tcgcctgaca atgtggctca tgattcctta ttatttatgg gtactattcg
     5821 caactctatt gaattattca atttattttt taaactaagc gctaaaaaga gacgtcctgt
     5881 aatttaacac atagaaaaag tgccacctaa gttagatttt ttgtctaatt taggtggcac
     5941 tctttattaa ccaaactatt tttttctagt acccacttgt tgttccatac tcattattct
     6001 ggtcataacc ataaccattt tgttcctgtc cagcgtaagg atcataactt tgagtatctt
     6061 gacttggtgt atagtactga cttgtatctt ctgtttgctg ataataggta ttcggatctt
     6121 ggtactgatc atatgtgttt tctgaatagg aagaataatc ggtgtaccct tcactattcg
     6181 atccatcacc gtttgtcgaa tcgccatctt ccgaatcatc aaagaacatc attgaagcat
     6241 tctcattttt caaattaggt aatgtatcgc ttgtttttat atttaattgt ttcttcagtt
     6301 cattttggat cgaaagaaga tcattttttc ctaaaatttg atagtagata tcattgatca
     6361 tttgtccttc gccttgcaac tggtcttgtt tgattgtttc aaacgccggg gtgtagttcg
     6421 tcgctacatc aagcatgtca tcccaatcta agtccgtcgt cacattgttc tcaactgctt
     6481 ttaaaatttt tctataattg cttacgccat ctaaacttaa aactttattg acgattttcg
     6541 tcactacttc ccgctgacga cgttgtcgac cgatatcgcc ttctggatct tcgtgtcgca
     6601 ttcgagaata ggctaaacct ttttctccat tcaatgtttg ttcacctact ggtacatgga
     6661 caccatccaa agtaaagtca attttattat tcactttgat gccaccgacc gcattgatca
     6721 aatcttccat gccatccaaa ttgatcgtta cataatgatc aataggaata tcgagcagtt
     6781 gctcaattga atccatggcc atttcgacac caccaaatgc gtaggcatga tttaatttat
     6841 ctaccgttcc ataacctacg atattcgtat aaatatctcg gtccaaactg atgatcgttg
     6901 attttttttg tttcggatta actgttacta ccatcatcgt atcagaacgt ccttgttccg
     6961 ttcttcctaa tccaccggta tctaaaccta atagcaaaac cgaaaaaggt tctttgtcat
     7021 caatgcttac tctctcagaa cgtttggagg atattcgatt agagttttta gaaattttgt
     7081 ttactgtttg gctcgcttct cccataaatc tgattccgta tgctgataat ccggcaatca
     7141 gcaaaacgaa tacccctaac aaggttaata gaactttttg ccatcgcttc ataatacacc
     7201 tctgtttcac attttctaaa atattgtctc atataactgt tcaatcagca aattcacttt
     7261 ttaatcataa gaaaacttta aaaaacattt ttgtgtaacg attattattt tttaaaattt
     7321 ctcaaaattc ttaactatca taccataccg tgaaaaagat cgaaagaccg aatacacgca
     7381 gttcgcaatc gttttatctt tttttatgta ttttttccag aaaaagcgaa caacaaggta
     7441 ttttccaaat aatcgtgcag taaaatgatc gcacatacct ttcccatctt tttaacagtc
     7501 tttgcctacc gttcagatta tcattcgatt tcacgacaat ttcttcctat gactggtcct
     7561 tttgttaaac ccatcttcta ttcgttttat ttccagctca ctgcatggct cttaaaatga
     7621 ctcgaactag tgattgcatt accatcatct tttaatggat agatatgctc ctcttcaaag
     7681 taaaacgatt ttcttttaaa gtaatagaat tgacatggta atgagtatgt atactagtgg
     7741 tttcacaaat ggtcttttat tcaacatcca aaaactgaaa tttctttaaa atccattact
     7801 aacgaagaaa atgttccttg cttgataatt agttaggaca tgtcaagtga acaaatgcat
     7861 ttacttagcc aaaaaatcta aaaacttcat aaatagacaa aaagacacga ctctctaaga
     7921 agtcgtgtct ttattcaaca aattattata atttagtcac ttcagcagct tgaggtccgc
     7981 gtgcaccttc aacaatcgtg aattctactt cttgaccttc ttctaggctc ttgaagccgt
     8041 ctccttgaat tgctgagaaa tgaacaaata cgtcatctcc gccgtctact gtaataaacc
     8101 caaatccttt ttcattgttg aaccatttaa ccgttccgtg ttccataaat gtatatcctc
     8161 caaaaaagtt cttgcaagta accttgcaag aacattatac ccaatttaga aaacgctagc
     8221 aagctttcgc tcttttattt tccaaattat ttttaagact gatatgacgg aggaaatggt
     8281 tattttgctg cttcgaaacg tttgtttact tcatcccaat taataacatt ccagaatgct
     8341 gcaatataat ctggtcggac atttttatat ttcaaataat aagcatgttc ccaaacgtcc
     8401 aaacctagaa ttggtgtctt tccttccatc aatggagaat cttgatttgc agtagaggta
     8461 atagctaatt ttccattttc cattacaagc caagcccatc cagaaccaaa tcgtccagcg
     8521 gctgcttttt tgaattcttc tttaaaagaa gaaaaatcac caaaagcttc attaatcgct
     8581 tcttttattt ctcctgtagg ttcgccacca gcatttggtg ccatgatttc ccagaaaaat
     8641 gaatggttag catgtccgcc accattgtta cgtacagctg tcttgatatc tgttggaata
     8701 gcgtccatat cagataataa ttcttctatt gttttttcgc ctaattctgg gtatttctca
     8761 attgctgaat ttaaattcgt cacataagta ttgtgatgct tatcatgatg cagatgcatc
     8821 gtttcttcgt caatataagg ttccaatgcg tcatatgcat aaggtagatc tggtaatgtg
     8881 taagtcataa aaaaattcct cctcaatgtt ttcttaataa tgtctacatt acaaagagta
     8941 acaaagagaa ggaaattctt caattgttat gcctagtcgt ctttttcact ttgacctact
     9001 tcttcttcaa aaacctcagt ttctttaaaa ataaacaact tgctgaaaat atagttcgca
     9061 aggataacaa taatttgagt gatgattttc gccacatact cattgctgtt cataacattg
     9121 atcaacaata ccattgcccc catgtccaat ccgtaagaaa ggatacggta gaagaagaac
     9181 ttaccgcatt caataagtgc atgtgtaaaa ttcggtgatt ttgaatggaa tacccaaagt
     9241 ttgttcgtta caaaagcaaa caatacagaa caaaaccacg aaatagaatt ggataaggcc
     9301 atacttaaat caaaatattg gcgtgcgaga gcaaaactaa caaaatttac aatcgtagcc
     9361 aaaccaccaa aaaagagata gactagtatc tcccaatatc cttttgcttc taaatatcct
     9421 tttaactgtc gatactgctt catgagccaa cctaccttat taaaaattct tatttatcat
     9481 actctatatt tcaataactt tcatctttat ttaaaaaaat taattttttt tgatcctttg
     9541 cctttgtctc tgcttggatc gtcacatgtg taataccata ttttaatttt aataaagaaa
     9601 ttatacgagg atatatcttt tcaatctcgc ttagtagctg atcttcaaaa tcaatatgtc
     9661 cgtcgaaaat aattatcttt tcgctatact gccaaatatg gacatgatga acatccttta
     9721 ccccatcgat tgcttcaatt tcttgttcca ttttttcata gtcgatagca ggtgcacttt
     9781 gtatcaaaat agcggcagct tttttgatca ccattatcgc ttctatcaat atatatatcg
     9841 aaatgatcag tgtaataact ggatcgatcc agtagatatc gaaaaaacgg atcaaaattg
     9901 ctccgataat cacacttacc gaagacagtg tatcacttaa caaatgcaaa taactagatt
     9961 ttatattcaa actttcatct gcttcttgtc gtaataataa tactgagaac aaattcgcga
    10021 gcaatccaat aactgctact gttagcatca aatgactatt tatcttttct ggtgaagtga
    10081 accgcttgaa tgcttcaacg gttaaaaaca aggataatat gatcaatatg aatgagttga
    10141 tgaaagcact caatatttct aaacgctggt agccatacgt acgtttttta tttgcttctt
    10201 ttcgggccat tttttgtgcc ccgtaagaaa aaataattgc caatgtatca ctaaggttat
    10261 ggaaagcatc tgaaacaaga gacaaactcc ctgacataat ccctccaata aattctgata
    10321 aagtgatcac cgcattcaaa accactactc caaatagttt ttttgatgat ctctgtggct
    10381 gtgtaggcac tattccttcc tccattttat ttatattgga tagtctttag acaagaataa
    10441 catttttttg atatttcacc aatcattcag ctgtttcctt aaagcacttg tatttcactt
    10501 gatacttcgc tagaattgca agtgatgaca ctactagatg aggagtactt atgacaacaa
    10561 aacaattaat tatcagttcc tttatacgat tcaaagacct aaaggaggcg agaaatgtcc
    10621 cattttggaa agccattgtc tatcttttag ttttgagtat aattatggct ttgccaatct
    10681 cttttcaagt ttttcaagtc ttaaacaaca ttcaattgga tgggcaaaag attgctactc
    10741 gcattcctga ttttacaatc caaaacgggc agcttcaaac cgaagaaaaa gaaggattca
    10801 tttaccaaac aaactccatt atcttcacat ttgatccaga gggaaaacga acagaacagg
    10861 acatctctac tgatttgatg gggaattttg ttagtgtcgg gatgttaaaa aacaaactta
    10921 tcatagctct tcctaataca ggcacgactt ctgctttgtt gaacaataac caattagaac
    10981 ttccctacac caatgaatct ttaaaaaact taacaggtaa gcagctccgt tcttttttaa
    11041 gtgaagcatc tataccactt tggataaaag tcctgacatt tatattttct atttatccta
    11101 gttttctgaa tctgctgatc actttgctat ttaccaacat tgctgctttt ctctatgcac
    11161 gctttcgact aacaaaagct acttttcttg attgtttaaa aacattgatc tattcaattt
    11221 cattgcctac tataattgca acaatcttga tggtctttct tccttctttt gacactagcg
    11281 ccttcattgc ttttgcagga atctttattt ttgctcaagc tgtcaaagga tgggcaaaaa
    11341 tatcgatcaa ttaagaaata aagacaactc gttatttcta cttcaataga cccttttaga
    11401 ggttgtgaca tttttttgtc acagcccctt ttttatttga aaaatatttc tttctcaaaa
    11461 aacaagcaag gcgcttcaaa gttcttcttg tatgcgctac acttagagta aagaaaggcg
    11521 ggaaattaac tatgtcagaa agaatcattt atgcagatcg tccatttcgt gatgaattta
    11581 tcgaagaaat ccatactata gcgcctggtt atatctttaa aacagaatta caaccagagg
    11641 atctacaaaa tgtagagatc agtttgggct ggaactcaga ctaccaagaa aaactattag
    11701 cctcttctac cttgaaatgg gtccaatcta tctcagctgg tgttgatcag ctaccccttg
    11761 agactttttc aaagaaacat attttacttt caaatggtag tggcatccac agtgaatcga
    11821 ttacggagca tgtcttagga atcatccttg ggtattctcg tggtcttttt caagcacaaa
    11881 aggcacagag tgacatgaaa tggttaggtt catcggtcca ttaccaatct atccaaggga
    11941 aaaaaatact aatcattgga actggccata ttggaaaaat gattgcaaaa aaatgcaaag
    12001 cattagacat gaactgctat ggaatcaata catccggtca tccagcagaa ggaatggaaa
    12061 agactttttc tttaaatcaa ctaaaggaag ttcttcctga agtagatttc gtagtcaata
    12121 tcttacctct aacggaggaa acaactgggc tctttgatca gcatatgttt gaacactttg
    12181 atccaaaatc tgtattcatc aatgttggcc gtggtgcttc tgttaagaca caagatctgg
    12241 tgcaggcttt aaataaccag caactttcct ttgcagcatt agacgttttt gaggaagagc
    12301 ctcttccaca agatcatcct ctttggaaaa tggacaatgt actgattact tcccacattg
    12361 caggtttgac acctgatttt caaaagaaat taatggctat ttttttaacc aatctgaaaa
    12421 gctactttgc cacccatgag cttcaaacca atcaagtaaa attgtctgca ggatattaat
    12481 agctgaaaac aatattttca aaagaaaaaa gagaaggtga caaaaattgt gtcaccctct
    12541 ctttttttat tgttttttaa agtgataggt tggctcctcc aggttgggat cataatcaac
    12601 aagtaaatca tatcctttaa agaaccattc atctgcttcc tcgataaaaa aaagtgtccc
    12661 atccatttcc actttcttta caggatgttc tggctgttca actgacattc caatcgaaaa
    12721 accatcatga acctctgttt tgccgtagac ttttccaaaa aaacgtattc cgtaagtttc
    12781 tggcagatca atttctgagt caaaccactt ttgtgctttt ggcgttaccg ttaatttcat
    12841 cccaacaacc tactttctct tttctttcta ttgattcatc ttatcgtcta gaatatccac
    12901 atcgcctgtc tctccaataa ccagtagatt atcattttca cgaacatatt catctgctgc
    12961 aggagaagcg ataaccgttt gatctactct tcgtatggcg actactgtta agtcaaaacg
    13021 ttgcctgaaa tttaattcag ctaatgtttt gttgaaaaat ttaggattgg agactcggat
    13081 ttctgctaaa gaatatttat ctgacaactc taggtaatct aaaatattct ttgacactaa
    13141 attatgcgca atccttattc ccatgtctcg ctcgggatgg acgacgcgat cagctccgat
    13201 cttttcaagt actctggcat gatactcatt ttgggctttt gctaaaatat ttggtacgcc
    13261 catttccttt accatcagag taactaagat actggcttga atatcttctc caatagcgac
    13321 aattacatga tcgaaatttc ttatgcctaa tgatcgaagt gtcatttcat cctgtgcatt
    13381 tgccacaaca gcatgagtgg ctatattcat atattcattg acccgatctt cattactatc
    13441 aatagccagt acttcttgac cagcttcgat cagtgttcgg cagatacttc cgccaaatcg
    13501 tccaagacct acaattgcaa aattttgttt caccgttttc ctcctttagt atcttctttt
    13561 ctcagtataa caaagccttt acaaaaacaa aagcaagcat cattattgta aaaaagccaa
    13621 gaaaaataat agttattttc tcagcttttg attatcctct ttatcatcgt ttttatttca
    13681 ccaaaccatg tttgaatgca tagatagcgg cttgtgtacg gtcttccact tctagcttag
    13741 ccaatatatt ggaaacatgt gtttttactg ttttcagtgt aataaacaat tcgtccgcta
    13801 tttcctgatt actttttcct tcagaaatca gcattaaaat ttcattttcc cgattcgtta
    13861 gttcttcatg taatacaggc tcatttcgcc gactcatttt ttccatcatt tttgttgtca
    13921 cttctggttc taatactcgc tcgccacgtt gcgttgccct tatagcatca gcaatttcat
    13981 gtgcggtaga ggtcttcaac aaataccctg ctgcacctgc ttcaatggca ggataaactt
    14041 tttcgtcatc aataaaactg gttacgataa tgattttagc ttctggccaa tcttttaaga
    14101 ttgcttttgt tgattcgata ccatccattt cttccatgac taagtccatt aaaatgacgt
    14161 ccggtcgaag tgtcatcgcc ttctcatagc cttgtcgtcc attttcagct tctccaatta
    14221 cttcaatgtc ttcttgaata gataaataag aagagacgcc taagcgcacc atttcatggt
    14281 catctactaa taaaactttt atcatcctta tctatgcctc cttcatcaaa gggaccttga
    14341 tttctacact tgtaccttga cctttaaaac taatgatttt tactgttcca ccaataccag
    14401 ctactcgttc tttgatattg ttcaatccat aacttcccgt tttcgtttca ttcatattaa
    14461 accctgttcc gtcgtcaata atacgtaaca aaagattatt gtctattttg tgcaaatata
    14521 cctctaattc attggcttta gcatgtctta atgtatttga taacaattct tgaacgattc
    14581 ggaacaagtg atcctcaatg gaactagata gttttacatc ttctacatcc catttcagcg
    14641 aaatctggat tttattttgc aattccttca ataactgctc gataccttgc tttaaacttt
    14701 tctcttctaa attgactgga cgtagatgaa gaagcagcgc acgcatctca gactgggaag
    14761 cgttgatgat ctctgctacc attttcaact gcttgcgaaa catttctggt gtctcgcttt
    14821 tttctgcttg ctccgtcaat gccgacatca tcatcattgc tgcaaacaat tgttgcgaaa
    14881 ctgaatcatg cagctcacgg gctaatcgat gtctttctaa ttctaaaatt tcttctttgg
    14941 tttgtccatc tacatattgg ggacggctcg ttacaatttg taattcacta gatacttcaa
    15001 tcattttttc ttttatctta gtgatttcct tatcaatatc tttgatgtat aaatcgtccg
    15061 atgcacttgg gatcggaaga agcaacaatt tggattcata attgccagaa gaaagtgcac
    15121 gcagcttttc ttcgattttc ccgtattttg tcttttgaat aagtgataat aatagaaaga
    15181 caattaatcc tacacctaag gaaatggcta acaaatggaa gataagcgga acatataata
    15241 aacgtgcacg cagtaattct ccccaccaat gactttgatt gctggcataa aaataagtga
    15301 aaagtgaaaa tagaataata aggaaagccg caatccccga ataaacagct agcatcgctc
    15361 tggatatttt tcccatcata ttcgtatcac ctcgacatct ccaatcaagg tattagtaat
    15421 aatcttcaat ctacgagggt tttcatcata atcactgctg tagattttga tttgttcatt
    15481 tttcaaagaa aattgctctt cttcaaacaa gacatttcct actaatgtcg catgctctaa
    15541 tcggatggct acgcccaaag gtactaagat ccgtgttctg cctatccctt ttcttacaat
    15601 cacaatatta tcatcttttg gcaaaagcgt attgcccaaa tctatgatag tatcaccaga
    15661 aatgacggcg atgttgatat cgtcccattc atagacgtcg tttccaatac gttggttacc
    15721 gaataattgc tgtcttttcc gctcattatt atgtgttttt aattgctctg tttggatcat
    15781 catgatctgt ttctttcgcc aaaaagcatt tttggttaaa tctatccctg aaatctctac
    15841 acctttcaga ccaataaata aaacggcaaa tacagccatc atccataaag caggactgtt
    15901 gactaagcta aaaaaaatga ccaaacttcc taatatcaat tgaaaatttt gaaatttagt
    15961 acgaggatgt ttgcgcatag ctagatatat attgaaaata ccgaatatca ctaaaagcaa
    16021 aagtccagta ttgtttacga tttgccaaac agcaaataac agcagtagtg cttcaactac
    16081 cacaaaaaaa cgccaagaac tattcatcgc tctttctccc tcctgacaca ttctcatttc
    16141 atcataccta tttttttaac tgttggctat aggactttag atgtaagatc gtttgttgaa
    16201 atctaaaata cacaaagaga aaaagatcta gagtgtatca tcagtaagat gaactctcta
    16261 gatctttcag tcagactacg cttgttctac tttgacgatt ttaactaaca tatcgccacc
    16321 tggagtagca atagttactt catcattttt acgtttgcca atcaatgctt gcgcaatagg
    16381 agaatcattg gaaattttgc cagaaaacgg atctgcttca gcgcttccga caatcgtgta
    16441 ctcttcttct tctccatctg gtagttcgat gaatgtaact gttttaccaa ttgatacctc
    16501 atctgcatct acaccgtcgt tatcgatgat ttgagcaaaa cgaatcatat tttcaattgt
    16561 tgtgattctt ccttcaacaa atgcttgttc atctttcgct gattcatatt cagagttttc
    16621 ggaaagatct ccgaaaccgc gggcaatttt gattcgttca atgatttctc cgcgtttgac
    16681 tgtttttagt tcttctaatt ctttttctaa tttttcttta ccttctagag tcattggata
    16741 aactttctct accatgtgta acgtctcctt ttttcgataa aataccgcaa aaggtaatct
    16801 tttgaggaat atagtccaaa aaagattacc acgcttttct tcaaatgtaa atgtttttct
    16861 aaaaatgatc aatgtctaga gcctgttttt ctaagaatta tttacatatt tttccactaa
    16921 ttccatatgt tcttcataag ttttagcgaa ataaacattt ccagtgctcg tatctgctac
    16981 gaaataataa tagtcatttt ctgctggttc taatactgcc ttgattgcct cttcgcttgg
    17041 attgtcaaaa ggtccaggtc catagccggt attggtatac aaattatatg gtgaatctac
    17101 agctgtatct tcatacgtca ctaattcttt gtgttcacct aatgcgtata aaacagagat
    17161 atctgactga agtggcatat ttgctttgat tcggttaaag aagacttgtg caatgttttt
    17221 acgatcattt tcttttacgc cttctttttc aactagagaa gctagagtta aaacttcttg
    17281 tacagtcaaa tttttctgct tgatagctga ataatagttt tgcatgaccg tattcgtctt
    17341 gttcaccatt tcaatcacta gatccttaag cgaagtactc gtgtaataat catatgtggc
    17401 tgggaataag taaccttcca aacggtattt tgtgttctca gctttagatg cactttccaa
    17461 cagttctggg aatttttggt gcaactcgtt aaagaaagtc tcatcattca ctaaatctaa
    17521 aaattctttc ttgtctttac cagttacctt cgctacccgt tcagcaatct gatcgatatc
    17581 gtacccttct ggaatggcaa ttttcccatc tgcaactttt gttggctcag aagtaccgcc
    17641 ttcttgcaac tgtttgccaa tctcgtcaag agtcatatta ggcgctaact gataatagcc
    17701 agcttgaaag ccagttaaat ttttaaattt tgtataataa ttgaaaacaa tcccgctttt
    17761 aataatattg tccttctcca aaatctcacc aatctgcttg ttagaagagc cactcgggat
    17821 ctcgacttgg actaattggt catctgattt atctaaaggc ttcaacccgc tatctacata
    17881 acgatacacg gtaaatccaa gaaatccgcc aataatcaat aatgtaagag caatcactaa
    17941 aatgatcttc ctcacaatct tatcttcttt tttacgcaga tttctccgct tgtcgattat
    18001 atctgctgta gctgccttac tggcttcttt tttatcttta cttgggtgtt gtttgtttcc
    18061 tgacaaaaca actcctcctt actatcaagt tcgtaacttt attcattata catgatattt
    18121 gaattggcgt gaatgatttt atttaaatat tctatcaaaa atgtaagtag taaatccatt
    18181 actataagat taaaataaat gttatcaaga tacaagctat tttagcttta ttctttctgt
    18241 aaaaaagaaa ggagactaaa taaactatta tgaaaaaagg aaaacatgct aaaaaaagaa
    18301 aatattcatt tttaatgatc gttgtcgtgt tgtttagcct tagtgcgttc tctacaggaa
    18361 ctattcttgg atggcaattt tacaataatg aacactctca gatagttgcc catgctcaat
    18421 cagctcaaaa aaagttgata ctgactacca atgaacaaca gctcgttgaa aaaatcgatc
    18481 aactattaag aaaaaatgat tatattggct caatttacgt aaagaaaaat aatcgcatca
    18541 tcatggaaaa aggctatggc tatgcgaata aactcacgca acaaccaaat gatccttctc
    18601 tttattatca gattggttcg gttcaaaaag cattgactac tcttttgatt ttaaaacaag
    18661 tcgagcgtgg gcacctttct ttagatacta agctggctca attctatcca caaattgctg
    18721 gcagccagca aattactatc cgtgacttgc tgtatatgcg ctcaggcttg caccgaactg
    18781 cttctccaac cataccgatg actgatgaag aagtcgttca atttgcgatt cagcacttaa
    18841 aatttacaga ttatcagacc tctcattatg attcattgaa ctttaccata ttgactggaa
    18901 ttttgatcaa attaacaaat caaccgtatg aaaaattatt gaccgatgat atcatcgaac
    18961 ctttaggatt acagcataca aaattttatg accaagtcaa aaattcatcc aatcatgcac
    19021 tatcctatca aatgtctaaa gaggatgatt actttaaacc actgacagaa tctgaaacag
    19081 agatccataa cgaattaggg actggtaata tcagtatgtc cgtctatgat ttaaatacgc
    19141 tgtttacaca agttttatct ggaaaactag tttccaaaga cttactcttc tcattatggc
    19201 gtgaaaatgc gaatggacat ccatatagtg gtggggtata tagcggagat aattatatcc
    19261 tgtcgcaagg aaatatcgac tcttttgata catctgctat atttagcaaa gattcacagg
    19321 atgctatcat catggaaagc aatataaaag ctgacaaaac tatcaaactg cctactgctg
    19381 atctaagaaa ccaaatctat gacctgatgg aaggaactac taagttggca aatagctgat
    19441 atgatgaaaa gatagcaaat actacttctt tgaatagcat ttgcttaaaa aaagtactcg
    19501 actaaacgta ggaaagagtg tgggacaaat ctaaaaatca atttttgcta cagactttaa
    19561 ttccaaataa acagaacaga aagaggtgtt cttctttctg ttctgtccaa attattctaa
    19621 gatttctctt taccaacatt atttgacaaa gggcctatta aatctaaaat agtatcttta
    19681 gattgctaaa atatgtgcta gcaaatcatt catggcttgt tcttattttt aattttatgc
    19741 aataaaaaag aagaacaatt agcgtgatta tagaaataaa taatgagatt ttagaaaaaa
    19801 atgtgtatga atacctgatt gttactttat ttattccact ttcacttttt actgtaacaa
    19861 aaggacctcc ttgttttagt actgtagcat tttttgaatt aacagtaacc gttaatcctt
    19921 tataccggaa tacaggtaaa actattgttt tgccttttga agatttaaac gtaaatataa
    19981 tttcatcttt atttggagaa aagctactat aaataacttg accatcttct gtaacttgat
    20041 ggtttttcaa aagcaattga tcttttttat agcctatagg tacataatca aaattctcaa
    20101 attcttcaag tttattttcc atttgttcat tagaataagt tccttgaaaa ttcatttctt
    20161 tcaccatgtt aactactgat gaataattta aaattgaaat agctaatatt aataatagta
    20221 taaaatttct tctccttgta taagaaaatt tttccaactg tggagaaata aatagagata
    20281 ctgaaaaaga tatgaacaat gttgaaaata tcataaaacg ccaaggagct tgaataatat
    20341 ttaaaggagt attttgtaag gcaaaccatg gaaatatttt tgtacaaagt agaatagata
    20401 ttatacccat tatataagtg attttagata agattcctaa ttttttaaag ttaaatgtta
    20461 gtataattaa acaaataagc aaagttaagc caataccaaa tgatagtaat tctgaatcta
    20521 tacttccaat caaaaactct ttacccagat aaattgctct tttttgtaat atatccacat
    20581 aaggtctatt cagatcttga tacaatgtct gctcaatcat aggacctata aacgctagtg
    20641 aaagaagtaa agtaactata gcagctttta ttaatttaat aattaagaag atttctattt
    20701 tctttttccg ataaaattga ataattaaag caattataat cattaccata gtcatagcta
    20761 cagaaagaac atgagtgtaa attaataaag tcatgctaat cgccaacata acccaagatt
    20821 ctttttttga gtcgtaaagc tttataaacc cgagtaaaat aagaggaagt attgacattg
    20881 cgagtaattc ccccattgcc cctcgaagaa atatttctac actcctaaaa gaagatgttg
    20941 tatatataat agcgaaaaag gagctagtaa catgtttttt ggtaatttca tacattacat
    21001 aatgacatat aaatagagta atgagcgtaa caatataaag aaaaaggtta tagcctattg
    21061 ctaaattagt aaacttgatc aataaaaata aaggatatag agttaaccat gggtaaaaat
    21121 tatttacttg catgccatat gatctaaacg tttcaaaatt aataggggag ctaaaaatag
    21181 attctaaact taacgcacga ttaatgtgaa atgccgtatc ataaaagcca aatatttcca
    21241 tgtttttaaa tgctggaaat aaatataata aactaacaaa tgc
//
LOCUS       DAJSBL010000045        21212 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.045, whole genome shotgun sequence.
ACCESSION   DAJSBL010000045 DAJSBL010000000
VERSION     DAJSBL010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21212
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.045"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(55..771)
                     /locus_tag="ONT85_001983"
     CDS             complement(55..771)
                     /locus_tag="ONT85_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HCR2378950.1"
                     /translation="MKKRILVTIGLAFLLAGCTNGNESKKESTKETAKATVATTTTTS
                     TESKSTAASTETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNAD
                     KSAQLEAFMTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEW
                     SESGTGQADYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFN
                     ETANNELKNGFNQIVYGQAN"
     gene            complement(998..1540)
                     /locus_tag="ONT85_001984"
     CDS             complement(998..1540)
                     /locus_tag="ONT85_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCR2378951.1"
                     /translation="MNIVEEKFVQETKQADIDHHKPTAGAMVGHVLANFKLEEMKLIQ
                     AQLYVLGTSSQELSTLFSEYADQEARWFNKISHELVIENEIVPSTLEEVMRYGKIYEH
                     GQNKYLPASTMLENFAKDFDFQNVFITRAIKLAEKEEKYGLQQLLVELLSFNKEIIYQ
                     VQRLQGKTVRQDLDEEDDDD"
     gene            complement(1545..1772)
                     /locus_tag="ONT85_001985"
     CDS             complement(1545..1772)
                     /locus_tag="ONT85_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HCR2378952.1"
                     /translation="MKKIIMQLDTLACPSCMQKIEHAVSQQPGVSNTKVLFNASKIKA
                     NFDPDEVHPEQLAAVVEKLGYEVQSAKVKEA"
     gene            complement(1851..3731)
                     /locus_tag="ONT85_001986"
     CDS             complement(1851..3731)
                     /locus_tag="ONT85_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HCR2378953.1"
                     /translation="MKLQKWIQEHRNHITALSAVLIIFGFIGKFTGFVLFYNIALAIA
                     SIIAVIPIMIHAYQAVKVKVISIDLLVTIAVLGAFMIGEYKESAIVTFLFLFGSYLEQ
                     KTLTKTRESIKALTEMAPSTASVVQEDGTTEEVEIDDVEEGDIVLVKTGGAIPVDGRV
                     IDGKGYADEAAVTGESRQVVKISGDEVFSGTILADGYVTIEATKVGDDTTFAKIIELV
                     EEAQDTKSHAEKFIDRFSQYYTPTVLLIALVVGLISRDFRLAITVLVLGCPGALVIGA
                     PVSNVAGIGNGAKRGILVKGGEAMDTFSKIDTFVFDKTGTLTKGTTAVSTIKNYTEDE
                     GDSLALVARLETLSDHPLGRAVIHYAQEKDIDFQQLAVKDNQTIKGQGITAEIDGHTV
                     CAGNTKLIAAHKLSLTPKQVQDLHQLQQTGSSVIIVAIDKKITQIIGVSDVIRPEVAE
                     QLAKLKQAGAKQLVMLTGDNQMTADYVADMLGIDEVHAELLPDEKVQFVKKYQEQGLR
                     VAFIGDGINDSPSLAAADIGIAMGSGTDVAIETSDVVLMQSNFESLVHAYRLAKKTVL
                     NTRENVVIAIGVVLFLLNGLFAGFIYMASGMFVHEASILIVIFNAMRLLYFGHESKEE
                     KS"
     gene            complement(3920..4534)
                     /locus_tag="ONT85_001987"
     CDS             complement(3920..4534)
                     /locus_tag="ONT85_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR2378954.1"
                     /translation="MKLTFKPLEKKDYKKAIYFAVEGMNFERYMENELLLNLYGRYFL
                     YMELNRATQILALYAEDELAGVLLAEIKGEKKKHRSFGQQIYVKIFDFLQNIFAKESK
                     GAYETANEQMLAAYLKKHSPDGEIIFLAANPDLKTKGIGTKLLTEFEKREKGKEIFLF
                     TDSGCTYQFYERRGFERLKEKISKIKILDKEIDLRCMLYRKIIQ"
     gene            4676..5236
                     /locus_tag="ONT85_001988"
     CDS             4676..5236
                     /locus_tag="ONT85_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCR2378955.1"
                     /translation="MKKTDRRVKKTEKALAETLSTLLVNKKIQAITIRELTETADVHR
                     STFYTHYKDIYDLYDQLESNFFRDLNEILSYDPAHSYEELYTRLIDYVYANTFLYRLF
                     ILNHGDISFQKKVKQVIEENYLNIWLYEDKKTIITEEMRFFTTYHVQGCLSVLNRWVE
                     SNYKYSKKELLELLQKLNDHIEKIMP"
     gene            complement(5541..5855)
                     /locus_tag="ONT85_001989"
                     /pseudo
     CDS             complement(5541..5855)
                     /locus_tag="ONT85_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
     gene            complement(6109..7744)
                     /locus_tag="ONT85_001990"
                     /pseudo
     CDS             complement(6109..7744)
                     /locus_tag="ONT85_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294751.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(8299..8862)
                     /locus_tag="ONT85_001991"
     CDS             complement(8299..8862)
                     /locus_tag="ONT85_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HCR2378956.1"
                     /translation="MITVVHKERQEKSSSDVRLWVYLGSISTKIQYGYTDSAKKQGNV
                     KFLRITDIQEGRVNWSSVPYCDISNSKLVDLRLEENDILIARTGGTMGKSFLVKEISE
                     ESVFASYLIRIRLVEKLLSEYVDCFLDSPLYWKLLEKISYGTGQPNVNGTNLSKLLIP
                     LPPLEEQQRMTTKIEMIRRSIRRINFE"
     gene            complement(8877..9920)
                     /locus_tag="ONT85_001992"
     CDS             complement(8877..9920)
                     /locus_tag="ONT85_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2378957.1"
                     /translation="MTQRVAIMILVLLVIGLLVYYVLKFKHWKQQRIHQDIEKKLKRY
                     PIVQAAWEKAEAKQYNIPGLTETRMVVPETGENEVCQWMTPQGLAFSQDFVFISAYCY
                     DHQHHSIIHVLDRETGQPIKLLILPKRPHVGGLVYDTKRELLWLTITGSATGRVAALR
                     LIDILADTSEETGQPIAYWLTTDLSEIPQASYLTQNNDQLVSGNFTLKGEGQLTFYLL
                     PTIAEMKTAIRRKDKIQPTVTMSRKTSDKIQGVAFYGHYLLASRSYGPYTSELLVSER
                     DSPSRILKRIKFPPYLEQIVVVEDRLAVLFESGAAAYREKANPVLANVLLLDLATLLH
                     ANKRPKKIAATKK"
     gene            complement(10053..10457)
                     /locus_tag="ONT85_001993"
     CDS             complement(10053..10457)
                     /locus_tag="ONT85_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HCR2378958.1"
                     /translation="MKKMYETAMINRGGRDGEVEAPNGSMHMKIDRPGIHSEGTNPEQ
                     LFAAGYASCFNGAVQHMLEENNLESDSEVKARVSLFQLEDGGYQIGVVLEVSLPGLEK
                     DQAEKIAEEAHAFCPYSKATRGNIDVEVTIVE"
     gene            complement(10690..11325)
                     /locus_tag="ONT85_001994"
     CDS             complement(10690..11325)
                     /locus_tag="ONT85_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HCR2378959.1"
                     /translation="MIFLQKYGFYFLLLGVMSDLSTPYILGLFYPKLNQMTTVISVFG
                     DVDSPVRRAFLVWSVVSGLFFVLSLPALYHLFVGTSKTLAILVVATVGLYGIGDCIFT
                     GLFSINTNESSCNLSTWIHNIGSGLGYAGFLLFPLLLVLLYRQSGQGTLSHFYLVLTV
                     ISLLIAGLYGLARIPSISQFAFFKQLGFFQRLSFFFNYLPMICLSFFQLRK"
     gene            complement(11591..12643)
                     /locus_tag="ONT85_001995"
     CDS             complement(11591..12643)
                     /locus_tag="ONT85_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCR2378960.1"
                     /translation="METSQKISPKIFLNKVLAGTATGIIIGLIPNAVLGAILKYFSAY
                     PFAVTIAQLAVIFQLATPLIIGGLIALQFGFDPMRMMVVAGASFVGSGVVKFTPDLGE
                     TGVYVGAGTGDIINTMLTASIAVGLILLIGNSFGSVSIVLTPIVVGVGAGLLGFYLFP
                     FVTAITSAIGSLINNFTTLQPLLMSILIGCSFAFLIISPISTVAIGMAIQLDGVSAGA
                     AAMGVAATTFVLVVNSWKVNKSGVTIAIALGAMKMMMPNLFRKPVILIPCLVSAVITA
                     IPVALFSISGTPASAGFGVVGLVGPLASLDAGLNGFLVVLCWLVIPVFAALITQFLCE
                     KVFKLYDREEVFKFLG"
     gene            complement(12656..13594)
                     /locus_tag="ONT85_001996"
     CDS             complement(12656..13594)
                     /locus_tag="ONT85_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015081805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HCR2378961.1"
                     /translation="MKIAIAGAGAMGSRFGLMLHQSGNEVLLIDGWAEHVQQIKEHGL
                     QANFNGKEVEAKLPIVLQSEVEKEDQVDLIILFTKAMQLEKMLQDIQSLIKKDTEVLC
                     LLNGIGHEDIIEKFVPMENIYIGNTMWTAGLEGPGQVKLFGSGSVELQNLGDGKEAAA
                     KKLADKLSESGLNAHFSDNIHYSIYRKACVNGTMNGLCTILDVNMAELGKTSTAHKMV
                     ATIVNEFAKVAAVEKIELDVPEVIAHCESCFDPETIGLHYPSMYQDLIKNHRLTEIDY
                     INGAISRKGKKYGVATPYCDFLTELVHAKEDSLNVK"
     gene            complement(14045..14695)
                     /locus_tag="ONT85_001997"
     CDS             complement(14045..14695)
                     /locus_tag="ONT85_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2378962.1"
                     /translation="MNDEYKNDEDKMLFEEIENRCRLNFELRGKMSLIQQKKYLANKS
                     EFTLGHVEKLISDWISSRSEFTKIKQPIKFDMKKLLLNKSEIGNRDQYIRAKGQEIID
                     SLGEMRSYNYLYVTHRADGMVITVGKSSSNDIFLDGDLFYQLNINHLSGTENIILRTE
                     YGNEIFAKYDEILKNYLDWAWIIPVESGDAKKLERLLGDELINKKVPILNYYSHRQ"
     gene            complement(14795..15304)
                     /locus_tag="ONT85_001998"
     CDS             complement(14795..15304)
                     /locus_tag="ONT85_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HCR2378963.1"
                     /translation="MNNNDRLLRLRYAIDLKDTDLIKAFELGGVPLTKEEAQAVLTKV
                     QDKNKDNAENNVYEKTINNQVFDAFLNGLILLARGPQKDKPIVDTPQKSKEIKYINNV
                     LLKKLKIALSMTTDDILDVFAEAEIYPSKGEIGAFLRKEGQRNFKPCGDKYMRNFLKG
                     LGIYNRRKV"
     gene            complement(15505..16374)
                     /locus_tag="ONT85_001999"
     CDS             complement(15505..16374)
                     /locus_tag="ONT85_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HCR2378964.1"
                     /translation="MYGSIEAGGTKFVCAIGNDDLKVLERVSFPTTTPNETMSLVIDF
                     FNHYKEQLESIGVGSFGPIDIHRESKTYGHITSTPKTAWKNFDFVGTLNKHFEIPIAW
                     TTDVNAACYGEYVSGHGKGLSSVVYYTVGTGIGGGAIQDGIFVEGFSHPEMGHTLVKR
                     HSGDTFSGTCPFHHDCLEGLASGPAIEMRTGTKGQDLSIEDPFWEIEADYIAQCACNT
                     TLMLSPDIIIFGGGVMQQEHLKKKVQRRFLELINGYVDTPNIEEYIVTPKLADNAGTI
                     GCLTLAKDVRINS"
     gene            complement(16394..17515)
                     /locus_tag="ONT85_002000"
     CDS             complement(16394..17515)
                     /locus_tag="ONT85_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HCR2378965.1"
                     /translation="MNCLNNIWLHKGEESRTISPENKNGEKGKAAMTASHLGIGRKGS
                     PSVPINAGETITLAEMEGPGILRHFWMTVTDKTIAGSFVLRDLILRIYWDDESTPSVE
                     SPLGDFFCNGFGTRCEVNSLPIVVNPTSGMNCYFEMPFRKKAKITLTNEHPEFIKHIF
                     YTFNYALTEVPDDAMYFHAKWNREPITTKGKDYTLIDGIKGTGYYVGTYFALCALERY
                     WWGEGEFKFYIDGDEDFPTITSTGAEDYFGGAWAFHKKNFGERAYAQNFNTLFMGYPF
                     QSKRDHTRDYFSAGKPNPNPVHGFGDDALPMHGLYRWHLPDPISFKENLRVTFQTLGN
                     DDIKLYERQDDISSVAYWYQKEPHEFFEKLLDREERIPR"
     gene            complement(17517..17912)
                     /locus_tag="ONT85_002001"
     CDS             complement(17517..17912)
                     /locus_tag="ONT85_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HCR2378966.1"
                     /translation="MRRLIIASHSILAEGFKETLEFLTGMHNQISSLCAYVEEIDDID
                     EQIKELVDTTDEIIVLTDMAGGSVNQKFFTYISDRVHVIAGMNLPLAMSIALQLGNDN
                     LDIGAIIAEAQQQIIYMNNQKSEERIEDE"
     gene            complement(17931..18737)
                     /locus_tag="ONT85_002002"
     CDS             complement(17931..18737)
                     /locus_tag="ONT85_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HCR2378967.1"
                     /translation="MNKMSEKYQVTKKDLRKANYRWLMSVCTFNYQTQQGASVTYALS
                     PILRKLYKDDDEYIEALNNHFQYYNTQPWLAAIILGACVAMEEKQGLEAKDAINDFKV
                     GTMGPIAGIGDSLLMTMIPTIMGSIAAYMALENNPVGIFLWFILIMVIFFFRMRGFEF
                     GYKQGMKIVTEYGEKINYLTEAASVLGLTVVGSLIASVISVSTPLKFTFGEVSMKIQP
                     MLDKILPTLIPVIITAAAYYLLSKKKISMTILILLVIVFAMVMAAFGILA"
     gene            complement(18730..19518)
                     /locus_tag="ONT85_002003"
     CDS             complement(18730..19518)
                     /locus_tag="ONT85_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCR2378968.1"
                     /translation="MHFTIFQIIMLTILAFIKHVDYYGIPMIFVNYAVFWGLFTGILM
                     GDIKTGLIVGGTVQLMSLGVAGFGGSSVPDYGTCAIIATAFGSQLGVEAGLAIGLPVG
                     MLGVQLDVIVKILNGFVVRKSQEYANKREFKKMNSVLWLGPIFFGLSAAIPVFIAVTL
                     GQSAVNYVLDVLPNWFMDGLTLAGKMLPAVGIAMLLRFMPTGRYISYLLVGFFLAAFL
                     KVPIIGAAIIGFALAFNVYHRAEKDAQITASAGASDVGFIGEDE"
     gene            complement(19532..20008)
                     /locus_tag="ONT85_002004"
     CDS             complement(19532..20008)
                     /locus_tag="ONT85_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCR2378969.1"
                     /translation="MSIVMSRVDNRLLHGIIVTQWAPMSKANRVMVIDDSVANNDIQK
                     SSMRLARPAGMAVSIITEETAIANFKKGKYDQEKVFVIVKEPETILHLVDNGIRIDRL
                     IVGGTVVKDNALQLSSRAYASDENISTYKKLLDSGVEIVTQFVPADKAQEIQSKLG"
     gene            complement(20080..>21212)
                     /locus_tag="ONT85_002005"
     CDS             complement(20080..>21212)
                     /locus_tag="ONT85_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCR2378970.1"
                     /translation="KIQKNVLVILFKLMCSKEFQQKRLALILAHGDLTATCIASTANR
                     IVERYIYDAIDMPLMTSSSEIADKINRYLQECTGYEEIILLVDMGSLEKIYLNLELDA
                     QKKFGLLNNVSTRMAIDVANQILLDLPLKQILEQVKENQNVDYIYESSNIKKDMIVCS
                     CASGLGTSFKLKKILEDSFPRDTDLVIETCDYYSLVNENYLSELRKENNILFIVGTLN
                     PHLEDVEFFSIEDMILGMNISKFSNQLNRHFNDNELKVLNNNILKNFSLTNLMEQLTI
                     LNPTKLLERVSDAIYILQNDLGISFDNNTCFGLYVHISCLIERLVKQNTLEDEIYFNE
                     TSEEFQKFQTHFKQSFSVVEHYYSVDIPIHEVKYVYDYVKRA"
BASE COUNT         6577 a         4350 c         3232 g         7053 t
ORIGIN      
        1 aaactgagca aggggttgtg ataaaagaca tgtcacaacc ccttgtttat ctcattaatt
       61 tgcttgtcca taaacgattt gattaaagcc atttttcaat tcattattcg ctgtctcgtt
      121 gaagtaaaga taattatcgg gattcccctg attttgttgt gtcaccaata cttttggctg
      181 attttgctga aacccgaata gatagacgtg ctttttcaga tatggttctg tctctgcatc
      241 ggaatagacg gctgtgattt gatagtctgc ttgacctgtc ccactttctg accattctgc
      301 agaaacaggc actccaccaa tagccatctt ccactccccg ttcaaaatag cttgagggac
      361 tttaagacca tataagtcca cactcatttg attcgtataa cttttgtatt gttgtcccat
      421 tgtttggccc catttggtca taaatgcctc taattgtgcg gatttgtctg cattccacag
      481 tgtttgttca acttgttcgc tgctttctga agaggaagat gaagcctcta cagaagaact
      541 tgaagtagat gtttgagttg tttcagtcgt tttggaagac tctgacgaag tagaggtatc
      601 tgctgtttct gtacttgctg cagttgactt gctctctgtg ctagttgttg tagtagttgc
      661 aactgttgct ttcgctgttt cttttgttga ttcttttttc gattcattcc cgttggtaca
      721 accagctaac aaaaaagcta gaccaattgt tactaaaatc ctttttttca attaaaaaac
      781 tcctttcgat tactcccact tctttattct atcaaataag caatgtgatt ttattgattt
      841 ttcgtatttt ctcatcaatc tttcaaatct tctaaaaact gagaagcacc cgctgatgtt
      901 gtctgtaata catcagcggg tgcttctttt attttaatct tcgtgatttt ccaatttacc
      961 tcacgcttta tatttgtatt tgaagaaaca tagactttta gtcatcatcg tcttcctcat
     1021 ccaaatcttg tcggacagtt tttccttgga gtctttgtac ttggtagatg atttctttat
     1081 tgaaacttaa aagttcaaca agcaattgtt gcaagccata tttttcttct ttttcagcta
     1141 atttgattgc ccgtgtgata aagacatttt ggaaatcaaa atcttttgca aagttttcca
     1201 acattgttga ggctggtaaa tatttgtttt gaccatgttc gtaaattttt ccataacgca
     1261 tgacttcttc taaagtagac ggaacgattt cattttcaat cactaactca tgagagattt
     1321 tattgaacca tctcgcttct tgatcagcat attctgaaaa aagggtactt aattcttgcg
     1381 aagatgtacc caaaacatac aattgtgctt ggatcaattt catttcttct aatttaaaat
     1441 ttgctaatac atgccccacc atggcgccag cagttggttt atgatggtcg atatctgctt
     1501 gttttgtttc ttgaacaaat ttttcttcca ctatattcat tgatttacgc ctcttttact
     1561 tttgctgatt ggacttcata acctaatttt tccacaactg cagctaattg ttcagggtgg
     1621 acttcatctg gatcaaagtt tgctttgatt ttgctcgcat taaacaaaac tttcgtattg
     1681 cttaccccag gctgttgaga aaccgcatgt tcgatttttt gcatacagct tggacaagct
     1741 agtgtatcta attgcataat aatttttttc atcgtagttc ttccttcctt tttcttgata
     1801 gtcttattct agattacagc ttccataaaa aaattgattc ttatcaagtt ttacgatttt
     1861 tcttctttgc tttcgtgtcc aaaatacaat aaacgcatcg cgttaaagat aacgatcaag
     1921 atactagctt cgtgaacgaa catcccacta gccatgtaaa taaacccggc aaataagccg
     1981 ttgagtaaaa ataagacgac accaatggca atcactacat tttcacgggt atttaaaaca
     2041 gttttcttag ccaagcggta ggcatgaacc aacgattcaa aattagattg catcaagaca
     2101 acgtcactcg tttcgatagc aacatccgtt cctgatccca tagcaatccc gatatcagcc
     2161 gcagccaaag agggagaatc attgattcca tctccaataa aagcaacgcg caatccttgt
     2221 tcttggtatt ttttcacaaa ttgaaccttt tcgtctggta ataattcagc atggacttca
     2281 tcaatgccta acatatcagc tacataatca gcagtcatct gattatcacc tgtcagcatc
     2341 actaactgtt ttgctcctgc ttgtttcaac ttagctaatt gttcagcgac ttctggtcga
     2401 atcacatctg acacacctat gatttgagtg atttttttgt cgattgctac aataatgacc
     2461 gagctgcctg tttgttgtaa ttgatgtaaa tcttgtactt gctttggtgt gagagaaagt
     2521 ttgtgagcgg caatcaattt ggtgttgccc gcacaaactg tatgaccatc aatctcagct
     2581 gtgattcctt gtcccttgat tgtctgatta tctttgactg ctaattgttg aaaatcaatg
     2641 tctttctcct gagcataatg gatcacggcg cgccccagag gatgatcgga taacgtttct
     2701 aatcgagcca ctaaagctaa gctgtcccct tcatcctctg tatagttctt gatagtggat
     2761 acagcagtag ttcctttggt caatgtacca gttttatcaa agacaaaagt atcgattttg
     2821 gaaaaagtat ccattgcttc accgcctttg actaagatgc cccgtttcgc tccattaccg
     2881 attcctgcta cattcgaaac aggcgcacca atgactaaag cgccaggaca accaagaacc
     2941 aaaacagtga ttgctaaacg aaaatcacga ctaatcagac caacaaccaa cgcaataagt
     3001 aaaacggttg gagtataata ctgagaaaat cgatcaatga atttttccgc atgggatttg
     3061 gtatcttgtg cttcttctac taattcgata attttggcaa aggtcgtatc atctccgacc
     3121 ttagttgctt caatcgtcac gtagccatct gctaaaatcg ttccagagaa gacttcatct
     3181 ccactgattt tgacgacttg tcgtgattca ccagtcactg cggcttcatc cgcataccct
     3241 ttcccgtcaa tgactcgtcc gtccacagga atagctccac cagtttttac taagacgata
     3301 tcgccttctt ccacgtcatc gatttccact tcttcagtcg ttccatcttc ttggacaaca
     3361 ctagcagtag atggggccat ctcggtcaaa gccttgattg actcccgcgt tttggtcaat
     3421 gttttttgtt ccagatagct accaaacaaa aacaagaatg taacgatagc tgactccttg
     3481 tattctccga tcataaaggc accaagcaca gcaatagtaa cgagtaaatc aattgagata
     3541 actttgactt tcactgcctg atacgcatgg atcataatag gtatgaccgc aataatagaa
     3601 gctatggcta gagcaatgtt atagaacaag acaaaaccag taaattttcc gataaaacca
     3661 aaaataatca acacagctga taaggcggtg atatgatttc gatgctcttg gatccacttt
     3721 tgtaatttca tctttctccc tcttttcctt tcattcttac gagactaagt atagatactt
     3781 atccatcata aaaaattgac cactatcaag tttagtcaaa aaggcggttt tcttttggta
     3841 gaatacacta caaaaaatag ttagcaagaa gactcactaa aagtgatctt gccaactatt
     3901 gtcgtttcta tcgaaatttt cattgaatga ttttcctgta cagcatacat cggagatcta
     3961 tttctttatc taagattttt atcttgctaa ttttttcctt tagtctttca aatccacgtc
     4021 gttcatagaa ttgatacgta cagccactat ctgtaaaaag gaatatttct tttcctttct
     4081 ctcttttttc aaattctgtt agcaacttgg taccgatgcc ttttgttttc aaatcaggat
     4141 ttgcagctaa aaaaatgatc tctccatctg gcgaatgctt ctttaaatat gctgcaagca
     4201 tttgttcatt tgctgtctcg taagctcctt tactttcttt agcaaatata ttttgtaaaa
     4261 aatcgaatat cttaacatat atttgctgtc caaaagaacg atgtttcttt ttttctcctt
     4321 ttatctccgc cagcaaaaca cctgccaact cgtcctctgc atacaaagca agaatttgag
     4381 ttgctcgatt caactccata tacaaaaaat atcttccgta aagattcagc agaagttcat
     4441 tttccatata tctctcaaag ttcattcctt caacagcaaa atagatagct tttttgtaat
     4501 ctttcttttc taagggttta aaagttagtt tcatcatttc gcctcctcaa cttcaatgat
     4561 tgtgttatac ttacattata atagacacag tgtctactat ctataatcaa tcatctataa
     4621 gtttgtcgat taagcgacag ctttgctttg atatgtcgga taaggagata ggtaaatgaa
     4681 aaagacagat agacgcgtca aaaaaacaga aaaagctttg gctgaaacac tatccacatt
     4741 attagtaaat aaaaagattc aagcaatcac tatacgcgaa ctaacagaaa cggcagatgt
     4801 acatcgttcg actttttata cacattataa ggatatctat gatctatatg atcaattaga
     4861 aagcaacttt tttcgggatc taaatgaaat tctctcttat gatccagccc atagctatga
     4921 ggaactatac actcgcttga ttgactatgt ttacgcaaat acttttttat atcgcttatt
     4981 tatcctcaac catggtgata tttcgtttca gaagaaagtt aaacaagtta ttgaagaaaa
     5041 ctatctgaac atatggctat atgaagacaa gaaaacgatt atcacagaag aaatgagatt
     5101 ctttactacg taccacgtcc aaggttgtct atccgttctc aatcggtggg tagaaagcaa
     5161 ttacaagtac tccaaaaaag aattattaga actcttacag aaactgaatg atcacataga
     5221 gaaaatcatg ccttgaataa aaacatacta agcttagtta agaagaaccg taaccttctc
     5281 gtataacaac tgaatcttat ttagccaaat tgtttactga agattttttg agtaaaattt
     5341 ctataattcc tacttattga aaagtagttt gtagtgctgc ttctccctct ctaagtgata
     5401 tatcttgttg taccaatcaa attcacctca tcattaataa attactttta ggctaattta
     5461 ttacaactta taggttgaag ctgtaaccaa tttatagtca tattatattt ttatcatcca
     5521 gtttattgaa ttcttctcgt ttacctcaat tcattttcaa ggggaatcag ctcatcatct
     5581 aacattttac gccatgctcg cttcatttct tgtgggtatt taaatggttt cgcttctgga
     5641 tgtttggctt tatatccttc gtaatcctta ctgttgataa ttgctttcat attaggaatt
     5701 ggatcttcac ctggcatata ttgcagatta gaagcatgta attttttctc agaaacaaac
     5761 cactcattgg aaaagacatg aatcaaatca tcacttactt gtgtaaaata gcgacgttta
     5821 atgacaaaga tatcttcatt gctatctagt gtaccttcat ttctttgtgc ttaaattcaa
     5881 agataatttt ctcaataaag atttttgatt ccagtaagat tcctttactc cttttacaac
     5941 atagcgttgt cctttgtttt gaaacatctt cttcccctct ttgccattaa aaatagccca
     6001 attctctata aagaagatta agcttcatta ttataatata aagtttaaat ttggtttatt
     6061 acttactttt tcttattctc tagtaagcta atattgaaaa aaatgtaatc agatcattat
     6121 ttttatatgt tgtaacttcc tgataagaaa actaattaaa agaaagaggt aactacataa
     6181 actaattaaa aaagctaact tcatagttgg aggataaatg gaagaaactt caatgattgt
     6241 ttctccttgt gttccttcaa tcgacactag tcctattatc aatctctctt ttttgtccat
     6301 ttacttttgc gactgttccc caataatcaa atacaggaag cgttattttt tgtcctttag
     6361 attcatgtac tgtatagact attttcgaat ccatcacttg ataaggcata gtatgccatt
     6421 ccttgccaat tcttacttca tgattctgca aatgttcttt gtattgtaat gcttccgtgg
     6481 gtgcatagtc ataaagtcct ccgggaagga gtgtcgtgta acgaataaga ttttcttgat
     6541 tcattaccaa gtgatctctc tgagtgacaa ctttatgttc atagtttgct tttgttgcag
     6601 cagatacagc caacagtaat aaaacaattg ccgttatatt aactactttt ctttgcttag
     6661 ctgatgtgct cttcatgaaa acgaacgata aaatcaacga gccaaacagt acttgaaagc
     6721 ctaagattcg ccaaggaaat tgaatcattt gaatgggtgt attttgtaat aaagaccatg
     6781 gaaaaaattt ggtggataag agccaagaga ctaccgcact aaggtaaatc actttttctt
     6841 tgcgattaaa tcctttaaaa aagaagagtg gacagatcaa agaaataata ccaatgattc
     6901 ctaccgtaaa ggttgtcagg tcattattca tcgcaccaat caaactatcc gagacattta
     6961 atgcttggtg ttgtaaggtc gttttatctg gctcattaat cgattgaacc atgatttgtc
     7021 ggaacatcgg ataccaaaag tatgctgtta aaagaatcgt cattcctact gcctttgaca
     7081 actccacaat caaccggatg ctttcttttt ttcttgtttc acttactaca aagccgccta
     7141 agactagtaa gccaacggct attacactca taaaagtgga aagaacatgt gtataaatga
     7201 gtaagctcat actgactgct aatggaattg cacatggctt tttctcataa atcagtgcgt
     7261 aagcataata cataactatc ggtataaccg taagtgcaat cgcttcagca atagcagaac
     7321 gatgataaat atcaatcgta cggtacccat aaaatgtata taaacagcta aatataaaag
     7381 ccgcatcgat tctttttaaa aattttagtc cataatgaaa catcagcatg atagtacaaa
     7441 cattcaatag ccaaacatat aagatataac taacgattaa attattactt atccaataaa
     7501 aaatgactgc cggaaaaaag gtcaagtagg gataaaaata attcactccg ctcccataat
     7561 gattaaacgt cgtaaaattg attggaccag aaaaaattga agataaccct ttgactctag
     7621 caaaatggaa ggcaatatcc cccgatgtag aaatagtatg aacatttcta aaataattga
     7681 ttgaataaag tccagcaata attgctagga gaatggacaa tattattttt ttctttactt
     7741 gcattactta attatatact cctttcatgg attgctttat ctagtataac ttaaatttta
     7801 aataaatcgt tatttatttt tactctcact taaaaaagca ctaaaattat aagagaggga
     7861 ggtaatggtc tgaacaccaa gaattgttcc aacatcgaat tgcttaacta atgagttcaa
     7921 agcttaatta tgtggctctt atatttaccg agcgtggatt gcgatctctt tagcgaaaca
     7981 aacatctttc ataaaaaaaa cagccatatc cgctttcttg gatatgacta tttactacta
     8041 ctttactatc tttcattctt cttgtctcac ttatgataag gcgctgcgta tcatgagtga
     8101 atgatattag gaaaacttca taaaataaat cttttataca tttccacttc tagatggtca
     8161 ttcatgttag aagaatcaaa ctattttttt actgtcgtaa taatattaaa acactagaac
     8221 aaatcaatta aattccataa aatataaata atagacctag catcatctgg tataaaatgg
     8281 atgctaggtc cgttatattt actcaaaatt gattctacga atagatcttc ttatcatttc
     8341 tattttagta gtcatgcgtt gttgttcttc taaaggtggt aatggtataa gtagtttaga
     8401 taaatttgta ccatttacat ttggttgccc agttccgtaa gaaatttttt ctaataactt
     8461 ccaatataat ggcgagtcta agaagcagtc tacgtattct gataataatt tctcaactaa
     8521 cctaattcga atcaagtaag aagcaaaaac agattcctca cttatttcct taaccaagaa
     8581 actttttccc atagttcctc ctgtccttgc tatcaaaatg tcattttctt ccaatcttaa
     8641 atcaactagt ttagaattac taatatcaca gtaaggcaca ctggaccaat ttacacgtcc
     8701 ctcttgtata tctgtgattc tcaaaaactt aacattgcct tgttttttcg ccgaatctgt
     8761 atagccatat tgaatcttag tagaaatact tcctaaataa acccatagtc gaacatcgct
     8821 agatgatttt tcttgtcgtt ctttatgaac aacagtgatc atcttgagtt gtccgactat
     8881 tttttagttg cagcaatctt tttgggacgc ttatttgcat gaagcaacgt tgctaagtct
     8941 agtaataaaa cattggctaa cacaggattg gctttctcgc gatacgctgc ggcgccactt
     9001 tcaaacaata ctgctagacg atcttctaca acaacgattt gttccaaata cggtggaaat
     9061 ttgattcgtt ttaggatccg gctaggagag tcccgctcgg acactaagag ttccgaggta
     9121 taaggaccat aagaacgact ggctaagaga taatggccat aaaaagccac accttgaatc
     9181 ttatcagatg tttttctgct cattgtcacg gttggttgga ttttgtcttt gcggcgaatt
     9241 gctgttttca tttcagctat cgtcggcaat agatagaaag tcaactgtcc ttcacctttt
     9301 agtgtgaagt tgccagatac cagttgatca ttgttttgag tcaaatacga tgcttgtgga
     9361 atctctgaga gatcagttgt cagccaataa gcgattggct gtcccgtctc ttcagatgta
     9421 tctgctaaga tatcgatcaa acgtaaagcg gctactcgtc cagtagcaga tcctgtgatt
     9481 gtcaaccata aaagttcccg ttttgtatca tatactaagc cccctacatg tggccgttta
     9541 ggtaaaatca gcagtttgat cggctgtcct gtttctctat ccagtacatg aataatagaa
     9601 tgatgctgat ggtcatagca gtaggcagaa ataaacacaa aatcttgaga aaaagccaac
     9661 ccttgcggcg tcatccattg acaaacctca ttttcgccgg tttccggcac caccattctt
     9721 gtttctgtta atccaggaat gttatattgt tttgcttcag ccttttccca agctgcttga
     9781 acaataggat accgttttaa ctttttttca atatcctgat gtatccgttg ctgcttccaa
     9841 tgcttaaact ttaggacata gtaaacaaga aggccaatga ccagcaacac taaaatcatt
     9901 attgctaccc gctgtgtcat ttttttgcct ccttgttcta ttcgatcaat cgtccatata
     9961 ccgctattaa ttaccgtaac tacatgtttc ttttactagc gtttctttat aggatctttc
    10021 ataatctcat ttatagtaac attaagaatc ccttattcta ctatagtcac ttccacatca
    10081 atattgcctc ttgttgcttt agagtatggg caaaatgcgt gggcttcttc tgcgattttc
    10141 tctgcttggt ccttctcaag tcctggcaaa gaaacttcta aaacgacccc gatttggtag
    10201 cccccatctt ctaattgaaa taaagatacc cgtgctttga cttcagaatc agactcaaga
    10261 ttattttctt ccagcatgtg ttgaaccgcg ccgttgaagc aagatgcgta tccagccgca
    10321 aaaagttgtt ccggatttgt cccttcacta tggattcctg ggcggtcgat cttcatgtgc
    10381 atacttccgt ttggtgcctc aacttcgcca tcacggcctc cacgattgat cattgctgtt
    10441 tcgtacattt ttttcattga gatcatcctt tctttcgtcg ttctatatta agtgtaacga
    10501 gtatttttta atctgacaaa ggtaatgctt gcattcggcg acatcagtta gacaacatcg
    10561 atgatcagtg atagaagcag tttatctata caaaaaaaga agggttctat gatatttggt
    10621 gtccttactc gccttgagta acaacaccaa atatcataga accacttaat cagcttcaca
    10681 ctgctttttc tactttcgta gttgaaaaaa acttaaacaa atcatcggca aatagttaaa
    10741 gaaaaaactt aatcgttgaa agaagcccaa ttgcttaaag aaggcaaact gactaatcga
    10801 cggaatccgt gctaatccgt aaagacctgc aattagtaaa ctaataactg ttaaaacaag
    10861 ataaaaatgg ctaagcgtac cttgaccact ttgacgataa agcagaacaa gtaacaatgg
    10921 gaaaagtaga aagcctgcat agcctagccc agaacctata ttatggatcc aagtcgatag
    10981 attacaagag ctttcattcg tattgatact gaataagcca gtaaaaatac aatcacctat
    11041 cccatataaa ccaactgttg caacaacaag tattgcaagt gtttttgatg ttccaacaaa
    11101 taggtgatac aacgcaggca acgaaagaac aaaaaaaagt ccagatacaa ctgaccagac
    11161 caagaaagca cgtctgactg gactatctac gtcaccaaac acactaatta cggtagtcat
    11221 ttgattcagt ttgggataaa aaaggccaag gatatacggt gtcgataaat cactcatcac
    11281 acctaataat aaaaaataaa aaccatattt ttgcaaaaaa atcattttct tcccctactt
    11341 tttagtgatt attcgataca tccaacaatc aaattttttc tcattttaac acatattttt
    11401 attctagttg tcttttgaac aatcaataac ataaaggtga gtttcttcta cattttaaaa
    11461 aatcgaatgg cactgaagag ttcagttgtc agcatcaatc tcctactcaa aaaggcaaaa
    11521 aaacgtaagt cagatgttgt tgacatctca cttacgcatc cattgattga aaatttctta
    11581 gtaaaatcta ctagcctaaa aatttgaaga cttcttctcg atcatacagt ttaaatactt
    11641 tctcgcataa aaattgagta attagtgccg caaaaacagg gatgactaac caacaaagaa
    11701 caacaagaaa gccatttagc cctgcatcta aagaagccaa cggaccaaca agtccgacca
    11761 cgccgaaacc agcagaagca ggcgtacctg aaatagaaaa tagagcgaca ggaatagctg
    11821 taatcactgc actaactaaa caaggaataa gaatcacagg ttttctaaat agattaggca
    11881 tcatcatctt catagcgcct aaagcaatcg caattgtcac acctgactta ttaaccttcc
    11941 aagagttcac tactaaaaca aaagtggttg cagctacgcc catcgcagcc gcaccagcag
    12001 aaacaccatc tagttggatt gccattccga ttgctactgt tgaaatagga gagataatca
    12061 aaaaggcaaa ggaacaacca atcaagatag acattaacag cggttgtaag gtagtgaagt
    12121 tattaatcaa tgatccaatt gcagaagtaa ttgctgttac aaaagggaat aaataaaaac
    12181 caagtaaacc tgcacctaca cctacaacaa tcggtgttaa cacaatcgac actgacccaa
    12241 aactatttcc aatcagtaaa atcagcccga cagcaatcga tgctgtcagc atcgtattaa
    12301 taatatcgcc tgtaccagca ccaacgtaaa cacccgtttc acccaaatca ggtgtgaatt
    12361 tgactacacc agaaccaaca aaagaagctc cggctacaac catcattctc atcggatcaa
    12421 atccaaattg taaggcaatc aaaccaccaa tgatcaaagg tgttgctagt tgaaaaataa
    12481 ctgctaactg tgcgatcgtt accgcaaagg gatacgcact aaaatatttt aaaatggcac
    12541 ctaatactgc attaggaatc aatccaataa tgattcctgt tgctgtacca gcgaggactt
    12601 tattcagaaa aattttcggt gatatttttt gtgaagtttc cattcaatct ctcctttatt
    12661 ttacgttcaa actatcttct tttgcatgga caagctcagt caagaaatca caataaggcg
    12721 tcgcaacacc atatttcttc ccttttctag aaattgcgcc attgatataa tcgatttctg
    12781 ttaatcgatg gttcttaatc aagtcttgat acattgaagg ataatgtaac ccaattgttt
    12841 ccgggtcaaa acaagattca caatgtgcaa tgacttctgg gacatctagt tcaatcttct
    12901 ctactgctgc tactttggca aactcattga caatcgtcgc caccatttta tgagcagtcg
    12961 atgtttttcc tagctcagcc atattaacgt ctaagattgt gcatagtccg ttcattgttc
    13021 cgttcacaca tgctttgcga taaatagaat agtgaatatt gtcagaaaaa tgagcattta
    13081 atcctgattc agacaatttg tctgctaatt tttttgcagc tgcttctttt ccatcaccta
    13141 aattttgtaa ttctactgaa ccacttccaa ataatttgac ctgaccagga ccttcaagac
    13201 ctgccgtcca catggtattt ccaatataga tattttccat tggtacaaat ttctcaataa
    13261 tatcttcatg cccgatacca tttaatagac ataagacctc tgtatctttt ttgattaatg
    13321 attggatatc ctgcagcatt ttttccagct gcatcgcttt ggtaaataga ataatcagat
    13381 caacttgatc ttctttttct acttcggatt gaagaacgat tggtagtttt gcttctacct
    13441 ctttaccatt aaaatttgct tgtaatccat gttctttgat ttgttggaca tgttctgccc
    13501 agccatcgat taataatact tcgttcccac tttggtgaag catcaaccca aagcgacttc
    13561 ccattgcgcc agcgcctgca attgctattt tcattgattc ttcctccttt ttctttctat
    13621 tatactcatt tgagaatctt ttcacaacct cgttttgtta ttcttctctt atttatcatg
    13681 tttatatcca ccattcaagt tattttatca gcaaatcaat aaaaactgga atagaatccc
    13741 aatttcatcg taagagaaac tagactagca ataagaaaat agaaacattc atagaaaaaa
    13801 tctcactcct ctgattataa aagagggggc attaacctcg tgtatactaa aaccttccat
    13861 tcattcgatg gatgattttc tttttgtgaa aaaatattca ttgtagtacg atgttgcgca
    13921 acacagacgg atggtattgc ttattattta ttaaaatcga acaatacact tatttaaaaa
    13981 cttctttccc agattttctt ccatttttac agttataact agtattatca tgaaaaatag
    14041 ccttttattg tctatgggaa taataattta aaattggtac ttttttatta attagttcat
    14101 cgcccaataa tctctccagt ttcttagcat ctcctgactc aaccggaatt atccaagccc
    14161 agtctaaata attttttaaa atctcatcat attttgcaaa tatttcattg ccatattccg
    14221 tccttaaaat tatattttca gtgccagaca agtgatttat attgagttga taaaaaagat
    14281 ctccatctaa aaaaatatca ttagaagaag atttccccac agtaatgacc attccatcag
    14341 ctctatgtgt tacatacaaa taattgtatg acctcatttc acctaatgaa tcaattattt
    14401 cttgcccttt agctctaata tattgatctc tattacctat ctcactctta tttaacaaaa
    14461 gttttttcat gtcaaattta atcggctgtt ttatttttgt aaattcgctt ctactactaa
    14521 tccaatccga gattaatttt tcaacatgtc ccagagtaaa ctctgattta ttcgctaaat
    14581 actttttttg ttgtattaaa gacatctttc cccgaagctc aaaattgagc ctacatcgat
    14641 tctctatctc ttcaaaaagc atcttatctt catcattctt atattcatca ttcataaaac
    14701 caatccagct cctcgattaa gataatattt taatctcata caaaaactag ctctcatcaa
    14761 aacaaccatt gttttgattt ctcatgaaat ttctttatac ttttctccga ttataaattc
    14821 ctaacccctt gagaaaatta cgcatatatt tatctccgca aggcttaaaa tttcgttggc
    14881 cttcttttct caaaaatgcc ccgatttcgc cttttgatgg atagatttct gcctcagcaa
    14941 aaacgtctaa aatatcatca gtggtcatag acaaggcgat ttttaatttt tttagcaaca
    15001 cattgttgat atatttgatt tccttgcttt tttgcggcgt gtctactatc ggcttatctt
    15061 tttgcggacc acgcgccaag agaatcaggc catttaggaa tgcgtcaaaa acttggttgt
    15121 tgatggtttt ttcatacaca ttattttctg cattatcttt gttcttatcc tgtactttgg
    15181 tcagtaccgc ctgagcttct tctttagtca gtggtacacc gcctagctca aaagctttaa
    15241 tcaaatccgt atctttcaaa tcaatcgcat aacgcaatcg caataaccta tcattgttat
    15301 tcatttgttc ctccgaagta tttagtcttt actttcttac gtactttttg tcttgctaag
    15361 atttttagga tcaatgataa aaaccgcatc acttatgata aggcgctggg tatcatgagt
    15421 gaatgatatt gctgaataca aatagtccga ccacattaag ttgatcggac ttcataagca
    15481 ttttattgac taatactttt ttgcttaaga attgattcta acatcttttg ctaaagttaa
    15541 gcatcctata gttcctgcat tatctgctaa cttaggtgtg acaatgtatt cttcgatatt
    15601 tggcgtatcg acatagccgt taattagttc taaaaaacgt ctttgtactt tctttttcag
    15661 atgttcttgt tgcatgacac caccaccaaa aattataata tctggtgaaa gcatcagggt
    15721 cgtattacag gcacattgtg caatataatc cgcctcgatt tcccagaacg gatcttctat
    15781 agaaagatct tgtcccttgg ttcccgtacg catctcgata gcgggtccag atgccaaccc
    15841 ttcgagacaa tcatgatgaa aaggacaagt accagagaaa gtatctcctg aatgtctctt
    15901 aactaacgta tgccccattt ctggatggct aaacccttca acaaaaatcc catcttgtat
    15961 agctcctcca ccaattccag ttccaacagt gtaataaaca acactggata aaccttttcc
    16021 atgacctgaa acatactccc cgtaacaagc agcattcaca tcggttgtcc aggcaattgg
    16081 aatttcgaaa tgcttattca acgttccaac aaaatcaaaa tttttccagg cagtttttgg
    16141 agtagaggtg atatgtccat aggtctttga ttcacgatgg atatcaatcg gtccaaatga
    16201 ccctactcca atgctttcta attgttcttt atagtgatta aagaaatcaa ttaccaaact
    16261 cattgtttca ttcggtgtag ttgtaggaaa actcactctt tctaaaacct ttaaatcatc
    16321 attgccaatg gcacaaacaa atttagtccc acctgcttct attgagccat acatttctta
    16381 ctccaccgtc cttttatcta ggaattcgtt cttccctgtc taatagtttt tcgaaaaact
    16441 catgtggttc cttttggtac caataggcaa cagaagaaat atcgtcttga cgctcataaa
    16501 gcttgatgtc atcatttcct agggtttgga aagtaacacg taaattttct ttgaaactaa
    16561 tggggtcggg taaatgccag cggtacaacc catgcatcgg taaagcatca tcgccaaaac
    16621 catgaactgg atttggatta ggttttccag cactaaaata atcccgggtg tgatctcttt
    16681 tagattgaaa aggatagccc ataaatagcg tattaaaatt ttgagcatat gccctttctc
    16741 caaagttctt tttatggaaa gcccaagcgc cgccaaaata atcttctgct cctgtagatg
    16801 ttattgtagg gaaatcttca tctccatcta tataaaactt aaattctcct tccccccacc
    16861 agtatctttc caatgcgcac aaagcaaagt aggttcctac ataataccca gtgcctttga
    16921 ttccatcaat aagggtgtaa tcttttcctt ttgtcgtgat gggttcacgg ttccatttag
    16981 catgaaaata catcgcatca tccggtactt cagtcaatgc ataattaaat gtataaaaaa
    17041 tgtgcttaat aaactcagga tgttcattcg ttaaagtaat cttagctttt tttcgaaatg
    17101 gcatctcaaa atagcaattc atccctgatg ttggattcac tacgattggt aaggaattca
    17161 cttcgcatct tgtcccaaac ccattacaaa aaaaatctcc taatggactt tctacagaag
    17221 gagtggattc atcatcccaa taaatacgca aaattaaatc acgtaagacg aaactcccag
    17281 cgattgtttt gtctgtcact gtcatccaaa aatgacgtaa aattccaggg ccttccattt
    17341 ctgctaaagt aattgtctct ccagcattta tcggaacaga aggtgaccct tttctcccaa
    17401 ttcctagatg actcgctgtc attgccgctt tccctttttc accattttta ttttctggtg
    17461 aaatagttct actttcctct cccttgtgga gccatatatt atttaaacaa ttcattttat
    17521 tcgtcctcta tcctttcttc acttttttga ttattcatat agatgatttg ttgttgtgcc
    17581 tccgcaataa ttgctccaat atctaaatta tcatttccta actgcaaagc gattgacatt
    17641 gccaagggaa gattcattcc tgcaattacg tgcactctgt cagaaatata ggtaaaaaac
    17701 ttttgattta cacttcctcc agccatgtca gttaaaacaa ttatttcgtc tgtagtatcc
    17761 actaactctt taatttgttc atctatatca tcaatttctt cgacatatgc acataagcta
    17821 ctaatttgat tatgcatacc tgttaaaaac tctagtgtct ctttaaaacc ctcagctagt
    17881 attgaatggc tagcaatgat caatcttctc ataattcaaa tccttttcct ttacgctaat
    17941 attccaaatg cagccataac catcgcaaaa acaataacga gtagaatcaa aatggtcata
    18001 ctgatttttt tcttactgag tagataatat gctgcagcag taataattac tggaattaat
    18061 gttggtaaaa ttttgtccaa cattggttgg attttcatac taacttcacc aaaagtgaat
    18121 ttaagtggcg ttgaaacact gataactgaa gcaattaatg atccaactac tgttagcccc
    18181 aatactgaag cagcttcagt taaataatta atcttttcac cgtattcagt aacaatcttc
    18241 attccctgct tataaccaaa ttcaaagcct ctcattcgga aaaagaagat taccatgatt
    18301 aaaatgaacc ataaaaagat tcctacagga ttattttcta aagccatata agccgcaatt
    18361 gatcccatga tggttgggat catcgtcatc aaaagagagt ccccaatacc agcaattgga
    18421 cccatcgtac caactttaaa gtcattaatc gcatcttttg cttctaatcc ttgcttttcc
    18481 tccattgcaa cacaagcacc taaaataatg gctgccagcc aaggctgtgt attgtaatac
    18541 tgaaagtgat tatttaaagc ttcgatgtat tcatcatcat ctttgtataa ttttcttaaa
    18601 ataggtgaca atgcgtaagt cacagatgca ccttgctgtg tttgataatt aaaggtacaa
    18661 actgacatca accaacgata gttcgctttc ctaagatcct ttttcgttac ttgatatttt
    18721 tcgctcattt tattcatcct ccccaataaa cccaacatca gaagctcccg cagaagcagt
    18781 gatttgtgca tctttttcag ctctatgata cacattaaat gctaaagcga atcctatgat
    18841 cgccgctcca ataataggta ccttcaaaaa agcggctaag aaaaatccaa caagtaaata
    18901 actaatgtat cgacctgtag gcataaagcg taacaacatc gcaataccaa cagcaggcaa
    18961 catttttccg gctaatgtta gcccatccat aaaccaatta ggtaaaacat ccaaaacgta
    19021 attaacagca gactgtccta aagtaacggc aataaatact ggaattgctg cagataaacc
    19081 aaagaaaatt ggccccaacc acaaaacact attcattttc ttaaattctc gtttattcgc
    19141 atattcctgt gatttgcgaa caacaaaacc atttaagatt ttgacaatta catctagttg
    19201 cacccctaac atacctacag gtagtcctat tgctaatccg gcttctactc caagctggct
    19261 accaaatgcc gttgcaataa ttgcgcacgt tccatagtcc ggtacagatg agccaccaaa
    19321 accggcaact cctaatgaca tcaattgaac tgtaccgcca actatcaaac ctgtttttat
    19381 atcacccatc aaaattcctg tgaataatcc ccaaaaaact gcgtaattta caaagatcat
    19441 cggaatccca taataatcaa catgtttaat aaatgctaat atagtcagca taataatttg
    19501 aaaaatagta aaatgcatta tccttactcc tttatcctaa ttttgattga atttcctgtg
    19561 ccttatccgc cggaacaaat tgtgtaacaa tttctacacc agaatccaac aattttttat
    19621 acgtgctgat attttcatct gatgcatagg ctctagaaga tagctgtaat gcattatctt
    19681 taacaacagt accgccaaca attagtcggt caattctaat accgttatct actaaatgaa
    19741 ggattgtttc cggctctttc acgatgacaa atactttttc ctgatcgtat ttcccctttt
    19801 taaaattcgc aatcgctgtt tcttcagtga taattgatac agccattcca gcaggtctag
    19861 ctaaacgcat acttgatttt tggatgtcgt tatttgcgac agaatcatcg atcaccatca
    19921 ctctgtttgc tttagacatt ggtgcccatt gcgtgacaat aatcccatgc aataaacggt
    19981 tatcaactct cgacataaca atactcataa atacttcctc ctttatcttt cattttctta
    20041 caagcaatta ccatgccaaa acaaaaagaa cttgataaat caagctcttt ttacataatc
    20101 ataaacgtat ttaacctcat gaatcgggat gtcgacacta taataatgtt caacgacact
    20161 gaaagattgc ttaaaatgtg tctgaaattt ttgaaattcc tcactcgttt cattgaaata
    20221 aatttcatct tccaaagtat tttgttttac taatcgttca attaagcaac taatgtgaac
    20281 atataatccg aaacacgtat tattatcaaa tgaaattcct agatcatttt gtaagatgta
    20341 tatagcatct gaaacccgtt ctaataattt agtcggattt aatatggtca attgctccat
    20401 taaattcgtt agactaaaat tcttcagaat attattgttc aaaactttca gttcattatc
    20461 attaaagtgt ctattcaatt gattggagaa tttacttata ttcattccta aaatcatatc
    20521 ttcaattgaa aagaattcta catcttctag atgtggattt agtgttccaa caataaaaag
    20581 aatattgttc tctttacgaa gctccgataa atagttttca ttcactaaag agtaatagtc
    20641 acacgtttct atgacaagat cagtatccct tggaaagcta tcttcaagta tcttttttaa
    20701 tttaaacgaa gttcctaatc cgcttgcaca agaacagaca atcatatcct tcttaatatt
    20761 tgatgattca taaatataat ccacattttg attttctttt acttgttcaa gaatctgttt
    20821 aagcggtaaa tctaataata tctgatttgc aacatcaata gccattcttg ttgaaacatt
    20881 gtttagtagt ccgaattttt tttgagcgtc tagttccaaa tttagataaa ttttctccag
    20941 tgaacccata tcaacaagta aaataatttc ttcatatccg gtacactctt gtaagtatct
    21001 attaattttg tctgcaatct cactacttga agtcattagt ggcatatcaa tggcgtcgta
    21061 tatatatctc tcaacgatcc tattagcagt acttgctata caggttgccg ttagatctcc
    21121 atgagccaaa atcagtgcta aacgtttttg ctgaaattct ttactacaca ttaacttaaa
    21181 taaaataacc aaaacatttt tttgaatttt tg
//
LOCUS       DAJSBL010000046        20981 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.046, whole genome shotgun sequence.
ACCESSION   DAJSBL010000046 DAJSBL010000000
VERSION     DAJSBL010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20981
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.046"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(193..1656)
                     /locus_tag="ONT85_002006"
     CDS             complement(193..1656)
                     /locus_tag="ONT85_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HCR2378971.1"
                     /translation="MKKTSKCLSALLLVGQVFGYTPAVLANEQGNNQLTNSQSATITE
                     ETTTNSTGEENQPETPTEDTIDTPDNPEMNAPPTGSEEQPETPNQPDKPEDNQQPEEE
                     PSEEPNEQPSDESSQTPQKPSTETEAEPPTKTERPQKAPEQSVTVPMNDVPQAQSIGS
                     EETKNYHQAPSAPIQELVPSSTQDNEGTIHFEKDASVETFIRKIGESARKIGQENDLY
                     ASVMIAQAILESASGQSQLAQAPNYNLFGIKGTHNGKGVSFATQEDLGNGTLYTTQAT
                     FRQYESYEESLKDYAKLLKEGLVGNTSFYDGVWKSKAKTYQEATKFLTGRYATDTSYD
                     KKLNGLIETYDLIKYDKEVAGPQLSKEGYMVPLKNYSISSSFGWRGEEFHRGLDMAAN
                     QGEPIYASKAGTVIEASYHTLSGNYVTIEHEDGSVALYAHQQEYCVNVGQTVEQGQII
                     GYVGSTGNSTGPHLHLELALDGSLTKDKLINPETVLF"
     gene            complement(1720..4737)
                     /locus_tag="ONT85_002007"
     CDS             complement(1720..4737)
                     /locus_tag="ONT85_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HCR2378972.1"
                     /translation="MFKRMATWLAIFVTFLNPMLGSVTAFAETINESNSTEEVMKTEE
                     SKSSEEAISTETTSETTEETTTQDTTEDVSPPNVMTQEVPEGYEKYFITIEDIQKATS
                     FSEQERLAQESEEWQAILEKNAQTRATNLTLTENGRKDVNMTRITPEGTMYTHDYMVR
                     WQIDGEDVFCIQEGAITSAGIQYDVTPLENLITTSAIRKKISLIGYFGYYSSGSMDNY
                     VLAQTYIWEVLGGSFVNYGTIGQSNYEAFKQTVDRKINNLLEDPSFDSQTVTVKVGEE
                     VEVKDTKGVFSQWDGSVVENTANVQVRKSGNSLFIKANQNSNDGRISVSSLDPNSRGV
                     GSSFAYVDPVAQNLGKLYVPDPNRAGLTIKVLKNGDFKLTKLDAVTGKKVPNVTYEIK
                     RHDTGETKQYTTDAKGEIALKEIPAFYEKDGKYVQNKLDVKEVKAPAGYILDKQTHTI
                     TIEPNKEVTLTAKNDLQQFIPEGKKVKEVYNAEESWNQGKPVYDTVPAENIEFDIIAK
                     TDITAPDKETVLVKKGEVLDSVKTDKDGHWKANKKFYNGDQNKYQLIEKNVPDNYRQP
                     SEEQTTFSVAYGDNTTPVLTFELDVIDNLLKTGEVDFNKVDSINHLQLPGAKFLIEGM
                     TAHNKNVSFVYETKEEPEKLKLPDGTYKFTEIVFPEHYGQASGQTETILVTVKDGEVQ
                     PITVKNEEIVVPEEDQPEISTLFVTTTGGKDFNPKKDQELVDKVSQVFPKSAVGTEKY
                     WVTQFHKITADGKVTVLDTVKETRKVTDEKETFDVTFNYKADMVSDGDTIVATHIVYN
                     DKEEEEEFARHFDLTNKDQTVTAKEPKIETRFITVNGDQTFDPDKDQELIDHVSQEFP
                     ESTVGETKYWVTQFHKIDKNGKDTVVGTVETARKVSKASEEFKVSFDYKAGTLKDGEK
                     LVATHIVYKDKDHKEEEARHFDLKNEKQTLTAKGIKKETPTTPVKQTPTSTPKQSLPK
                     TGETVNDVLIALGLFLFVGVGAVLVRRKMKQSN"
     gene            complement(4888..5727)
                     /locus_tag="ONT85_002008"
     CDS             complement(4888..5727)
                     /locus_tag="ONT85_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HCR2378973.1"
                     /translation="MKMLKVLALGIVVGGVSLAVGTNAEAAENKVWTPRSAEQIKAEI
                     KGNEYTIVWGDTLSAISQATNITVQKLADMNKITNVDLIYAGNKLVFNGNVASVQNSA
                     GETVAQTVIQEKDKVDPNQPVGQGTTSNTQTTPNTTTPTTPAGDQTGTTGGTTTPSTP
                     ENNGGTTQTPTQPGNGGNTGGETTPSNPSTGGGTTTPEEPSNPGGGDQGGNGGTTTPE
                     EPTIVVGEIGNSGKVFNTVDEAYDWANAEVSDPNSQWYNKGFMVDVIIYSDGSQKHTV
                     DFY"
     gene            5872..6117
                     /locus_tag="ONT85_002009"
     CDS             5872..6117
                     /locus_tag="ONT85_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014862476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2378974.1"
                     /translation="MQLNNLNEIHDWAQNNLLNRKQAAKITGQNYDAFSSSLRRNNIK
                     PFLEIGDEPGPSIIRLYLREDIEKYAKQLEEKRQKRQ"
     gene            6180..6602
                     /locus_tag="ONT85_002010"
     CDS             6180..6602
                     /locus_tag="ONT85_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014862359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2378975.1"
                     /translation="MNKKYIVSVGSIVLIVLAIIAFSVSNKTETQPQNTVQSTTTSQS
                     SDHSVYPSLNSLKETHGQDLIDLGSSQITTVLFEKKNIAFITPKEGTDLEFSIKVTNT
                     KENLFSIPDIQYTPNKLKIGDTFGLAKVNEQYFAYSLK"
     gene            complement(6696..7013)
                     /locus_tag="ONT85_002011"
     CDS             complement(6696..7013)
                     /locus_tag="ONT85_002011"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2378976.1"
                     /translation="MPVKGTSNFDFEIFYVDDEICGVFNKQKYTKEQAIEAWKNELFD
                     PEEEISFVVDESFVGWYHGNAEEEEVHYAGWWLEEGEPREQSVPVWVISENPLEEIEK
                     LEK"
     gene            complement(7040..7246)
                     /locus_tag="ONT85_002012"
     CDS             complement(7040..7246)
                     /locus_tag="ONT85_002012"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2378977.1"
                     /translation="MNWLKNEAELYVFHEEMQEKHLVPNVRNYLFWLEEKNDAQVFGK
                     SAQSPKIKLQLTSKQWNEHEWYIY"
     gene            complement(7227..7409)
                     /locus_tag="ONT85_002013"
     CDS             complement(7227..7409)
                     /locus_tag="ONT85_002013"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2378978.1"
                     /translation="MTKCERVSVTISFIALLLSVISLLNTAIFIGQVEKQKEISLSEW
                     RYEEVNEGMNNELVEK"
     gene            complement(7423..7773)
                     /locus_tag="ONT85_002014"
     CDS             complement(7423..7773)
                     /locus_tag="ONT85_002014"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2378979.1"
                     /translation="MKKSYKVFENNEIKEQEAIILSAQTPEEALEQALPFLVESQNIV
                     VEELPEFDSKIVFSFNCKKESEISVSPEFYCRSDISVEGLVMCASAFIEIFANHLQIS
                     VKELLDLLQEITNE"
     gene            complement(7799..8101)
                     /locus_tag="ONT85_002015"
     CDS             complement(7799..8101)
                     /locus_tag="ONT85_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2378980.1"
                     /translation="MLSKQEKKYIRQKMYAMIGNRAITELSSEELLQVQQLAQIIGSN
                     SIFDSNPLPEMTLETLTAKRYKELRAIGYRMIDIRRALRISDTKFREWRTSNGLAN"
     gene            complement(8104..8337)
                     /locus_tag="ONT85_002016"
     CDS             complement(8104..8337)
                     /locus_tag="ONT85_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2378981.1"
                     /translation="MTISVGNKVKYIGTAIPRYTNKLVVVKNILVNGLILEYPKEDRR
                     KVVVEGNGTWNMNSFICGFTEVEVTNDNENRSG"
     gene            complement(9323..9645)
                     /locus_tag="ONT85_002017"
                     /pseudo
     CDS             complement(9323..9645)
                     /locus_tag="ONT85_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347542.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(9768..10883)
                     /locus_tag="ONT85_002018"
     CDS             complement(9768..10883)
                     /locus_tag="ONT85_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HCR2378982.1"
                     /translation="MATKRFTATRKYNELYYQFPKVFITSEKYRSLSDKAKIAYMVFR
                     SRLDLAIQRNQVDSQGFVYFEFTEQELANALNCCRRSVNTIKKELKEYELLEIEDMGF
                     DQQTGKRNKARLYLGELDTTENDVYSLGPVDSTSVDSAQGKEEEESVDNLAIFDDFDE
                     KNPCENSAQLFNNSSNNLDTENLDTKIDTTSPVEMADDWQKEFIAHYSESTFVPKDVL
                     TWIVRSSAEWREAKEQLNMIYDSKRIIEKRQRKYNQTHGLSDGSEYRILGETFAEDLS
                     KTYQRCIQNEKIARHEGKAIQNRKGFYFRALLNFWECCVRELNLDPMATKLSDQAEYS
                     LLQAKRSCSYEDLETQIYGLSQLPKISMHNWLEELDT"
     gene            complement(10889..11287)
                     /locus_tag="ONT85_002019"
     CDS             complement(10889..11287)
                     /locus_tag="ONT85_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HCR2378983.1"
                     /translation="MVKNKFRATDVLTQATFHMPKLFFIKSSKYYAMTAQAKYAYSLL
                     NEYLEESLIEKRIDEKKHVYISYTNQELAILLNVSEEEIEEIKNELEDYDLLTIEFGR
                     IYLKELIATMEDVRVYQHQNSFLIYGQDEV"
     gene            complement(12316..12702)
                     /locus_tag="ONT85_002020"
     CDS             complement(12316..12702)
                     /locus_tag="ONT85_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HCR2378984.1"
                     /translation="MNKRKTEFILILISGITGILCSLLALMGGMFLSSIDSTTLNTTE
                     LTSKDIDMLVNLSGNVVLFGGITLVFSIMIFIIGFLVRKNQKTVVYGISLLILSIIPF
                     FLLTLLWVIPGVLGIIAGLMLLIRKS"
     gene            complement(12699..13220)
                     /locus_tag="ONT85_002021"
     CDS             complement(12699..13220)
                     /locus_tag="ONT85_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HCR2378985.1"
                     /translation="MNKKSGIETLPNYVFAGFWLRFFAFLIDLICISIITHATIGLFY
                     KIMGLESDKSIITFYGIASLAIYLTYFVLLTKLNSGQTIGKMIFGIRVVSFEEKELSW
                     QTIFIRECICRFILKNFVFQIGYLVAAFTPKKQHIGDYFSNTSVVVIRSLEAINYGQK
                     ISNSKTLNLERIK"
     gene            complement(13222..14256)
                     /gene="sppA"
                     /locus_tag="ONT85_002022"
     CDS             complement(13222..14256)
                     /gene="sppA"
                     /locus_tag="ONT85_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HCR2378986.1"
                     /translation="MNKRRWIAVGVACGLLLLSIIVALIPGKDKEEASNTTLTGINKI
                     FYGSNEITEETLEEGASNKKIVKLSVNGVIADTGESNLFSREQYNHQNFLTQLKKIQE
                     DKAVKGVLLEVNSPGGGIYESAEIAKEMAKIKKLDIPIYTAFKNTAASGGYYISAGSD
                     KIFATEETTTGSIGVIISGLNYSGLLEKLGVTDATYKSGALKDMMSPQHKPSEEENKV
                     IQEFVMSAYDRFVNVVAKGRNMDTNAVKELADGRIYDGNQAVENGLVDQIGYSEDALD
                     SLKKEKKLTDATVIEYKNDTTGFASSWLGNKIAEWQGLKATTSDRVISVFEKLGFSDT
                     PKPMYYYGGL"
     gene            complement(14289..15704)
                     /locus_tag="ONT85_002023"
     CDS             complement(14289..15704)
                     /locus_tag="ONT85_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCR2378987.1"
                     /translation="MVSIKNYGKSRRGKQFMQNNNWFEHSSIYTKQHHSFYRWVLYPV
                     ILFLCIIGLFLTFAKKEVVIRTTAQLNPEKIEKLQVPLEAKIMENFLKENQFVHKDDV
                     LVRLDCSLIENEKAQIEQENQRITQQIKMAQLFIESISKGKNLFSTDDSFGYSNQLKS
                     MLSEKESLRYALKQSELNDQKQLEVYEKTKRQLEKQIESSDSKLQEWQQVQVAWSNNQ
                     SLKDFSKEMMANYENWQEQLNNVSDDQKNQVKLTISASINEQIEQLKKEVEQYQSEKA
                     KLVKPTTSENDRISQTEKGKQELEQAITTVKQTVTELNEKQEKNQSVIKSLDDQLSKG
                     ILKAPVSGTVHLNEETKGQAELAKGTVLAEIYPIHEKSKMKFTALLPANESTYIKEGM
                     KVHFKLDQKGGAPITIDGTLDEISENSTATEKGVFYAVKGTLKTTQSFPFRYGLTGEL
                     SLIVGEKTYWQQIVDVFLNKK"
     gene            complement(15673..17826)
                     /locus_tag="ONT85_002024"
     CDS             complement(15673..17826)
                     /locus_tag="ONT85_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HCR2378988.1"
                     /translation="MIKSFPFVQQQDEKDCGVACLSMILKYFKTDIPIHKLRDLSGTD
                     LEGTSAFGLKKTFEKLSFDCMAIQADSEIWKERDLILPLIAHILVDNSYMHYVVIYKT
                     KGNVLWIADPANGKNRKTIEAFQKEWTGILLLPMPRETYIPIKEKVAGLSSFLPIIWK
                     QKGLVFHIVLASIFITFFGIGSSYYFQGILDFFIPNQARSTLNIISIGLIAVYLFRVL
                     FEYSRSYLLIILGQRMSMAIMLQYFKHVLTLPMNFFATRKSGEIISRFLDASKIIDAL
                     ASATLSIFLDIGMVLLVGVTLAIQNSTLFLITLCSLPFYVVAIFAFVKSYEKSNREEM
                     AAGAAVNSSIIESLKGIETIKAYSGEEKVYNRIDHEFVKLMKKSFRTVTLDNIQQGIK
                     HLIQLISSALILWLGSYFVMDNRISLGQLITYNALLVFLTDPLQNIINLQVKMQTAQV
                     ANNRLNEIFAIDSEKSNQSFEYMLSEQLFQQDITVKNVSFSYSMKAPTLNEISCTIPS
                     HSKTAIVGVSGSGKSTLAKLFVRFYEASEGSIHYGNINHLDIPFQILRDRVTYLPQES
                     FFFSGTIIENLIFGLDSPPSFEQIIEVCEIVQLNEFINQQPLRYETLLEEGGNNLSGE
                     QRQRLALARALLKNPDILILDEATSGLDTLLEHAIMNYLLNLKEKTIIFIAHHLPIAK
                     ACDQILVLHEGHLVEKGTHDELRFADGIYQKLWEI"
     gene            18084..18320
                     /locus_tag="ONT85_002025"
     CDS             18084..18320
                     /locus_tag="ONT85_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HCR2378989.1"
                     /translation="MFKHAKCKGTKYCGNAQTVGGGNNAWGKLGQVVGGLTTGAVGGA
                     GLGTAICGPACGVVGGLYGAVAGGAAAGWDARKK"
     gene            18339..18554
                     /locus_tag="ONT85_002026"
     CDS             18339..18554
                     /locus_tag="ONT85_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Blp family class II bacteriocin"
                     /protein_id="HCR2378990.1"
                     /translation="MHIKNTKTTFILSSEELKNIQGGSAVGVLGTTFSGATAGVKLCS
                     AGGPYAIAACGVGGALLGAGFSMWTGA"
     gene            complement(19257..20009)
                     /locus_tag="ONT85_002027"
     CDS             complement(19257..20009)
                     /locus_tag="ONT85_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR2378991.1"
                     /translation="MSYPIILCEDQIIQLNQLERIIDNFILFHDKVFKIVLKTQSPLE
                     VKKYLKQFRPKQGIYFLDIDLNHEVNGIELAEVIRKYDVQAKIIFTTTHDEMLPVTIK
                     RRVETLGFVTKDQTLDEYRNEIVELLLLAQERIDATKQVTNQAFVFSIGSQTFTFDIN
                     DIYFVEASKLPHRLSLCTKDGQYEFYGRISELEKKYPMLTRISRACLVNIFNVKEIDF
                     KKRSLYFDSELARNFTLGKAQKIKEKLKESTV"
     gene            complement(20024..>20981)
                     /locus_tag="ONT85_002028"
     CDS             complement(20024..>20981)
                     /locus_tag="ONT85_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HCR2378992.1"
                     /translation="KGYGYVVFQIMLDSLFLLMFIWLYRKLAINRIVEQSSSKLTAIF
                     MIYLLTIGLFVSYVAHQYQVFDHFVFGVLIFLIIQMFVVLFLFIRITIRQREQYEQQL
                     KRKELVYLKKYTDSLEKDQEKFARFRHDYKNLLLSLKEIANQNHDSILEKEIKELEIY
                     SNSYLTNKFEYRYLKNIKNEYLKSLLIAKLYQANNNKICCKFECPSTIIEVPVPIFDC
                     IRVLGIILDNAIEASIESQDRELSLAIYRDDKQIEISISNSCREILLSIDTLMKKGIT
                     TKKGHQGLGLSSIEEINKKNPNMFVNYQNDPYQFTTQVILLF"
BASE COUNT         6137 a         4032 c         3105 g         7707 t
ORIGIN      
        1 tttgagtaaa gaaataagtc aatgaagacc caaacgataa aaaatattcc taatactata
       61 tttaataaac tacgttctag atagatacga gatttcaaat taaaaaacga aaatgagaga
      121 tacaaatata tcaaatattt aaaaagtaac cacattttct ataccactcc ttatcagaaa
      181 cagccttatt ctttagaata agactgtttc tgggttgatt aatttgtctt ttgttaaact
      241 accatctaag gcaagttcta aatgtaaatg tggtcctgta ctatttcctg ttgaacccac
      301 ataaccaata atttgtcctt gttcaactgt ttgtcctacg ttcacgcaat attcctgttg
      361 gtgcgcataa agagccacac ttccgtcttc atgctcaatt gtgacataat ttcctgacaa
      421 agtatgatac gaagcttcta tgaccgttcc tgccttgctc gcatagattg gttctccttg
      481 attggctgcc atgtctaatc ctctatgaaa ttcttctcct cgccaaccga aggaactaga
      541 aattgaataa ttttttagag gtaccatata gccttctttg cttagttgtg gaccagctac
      601 ttctttatca tatttgatta agtcataggt ttcgattaac ccatttaatt ttttatcgta
      661 gctagtgtct gttgcgtatc ttcctgttaa aaatttagtc gcttcctgat aggtttttgc
      721 cttacttttc caaacaccat cataaaaaga ggtatttcca actaagcctt cttttaacag
      781 tttggcatag tcttttaagc tttcttcata gctctcatat tgccgaaaag ttgcttgggt
      841 cgtataaagt gtcccattgc ctaaatcttc ttgtgtcgca aaagagacgc cctttccgtt
      901 atgtgtgcct ttgattccaa atagattgta gtttggtgcc tgagccaatt gactttgtcc
      961 gcttgcggat tctaaaatgg cttgcgcgat cataacagaa gcgtataaat cattttcttg
     1021 tccaatttta cgagcagatt caccgatttt acggataaac gtttctacag acgcgtcttt
     1081 ttcaaaatga atagtccctt cgttatcttg tgtgctggaa gggactaatt cttgtatcgg
     1141 tgcgcttggt gcttggtggt agttttttgt ctcttctgaa ccgattgatt gtgcctgcgg
     1201 tacgtcattc ataggtacag tcacactctg ttcaggtgcc ttttgtgggc gttccgtttt
     1261 tgttggcggt tccgcttctg tttcggtact tggtttttga ggagtctgtg agctttcatc
     1321 agaaggttgc tcatttggtt cttctgacgg ttcctcctca ggttgttgat tgtcttctgg
     1381 tttgtctggt tgattcggtg tttctggttg ttcttcagaa cctgttggtg gagcgttcat
     1441 ttctggatta tctggtgtat ctattgtatc ttcagtcggt gtttctggtt gattttcttc
     1501 tccagtggaa tttgttgtcg tttcttcagt gatcgttgca gattgcgaat ttgtcagttg
     1561 attgttgcct tgttcattag ccaaaaccgc tggcgtatac ccaaatactt gacctactaa
     1621 taacaaagca gataaacatt tactcgtttt tttcacaaga ttgcctccat tcttcaatca
     1681 atcatctaga gacagctgat tgctgtctct agtcatactt taattgcttt gtttcatttt
     1741 acgacgaacc agaacagccc ccacaccaac aaaaagaaac agtcccaatg caattaacac
     1801 atcgttcact gtttctcctg tttttggtaa gctttgtttt ggtgtgctcg tcggcgtttg
     1861 tttgacaggg gttgtcggcg tttctttttt aatgcctttt gctgtcaatg tttgtttttc
     1921 attcttcaaa tcaaagtgac gtgcttcttc ctctttatgg tctttatcct tatagacaat
     1981 atgagttgcg accaatttct ccccgtcttt taacgtgcct gctttgtagt caaaggatac
     2041 tttaaactct tcagacgctt tagaaacttt acgtgcagtt tctaccgtac caacgactgt
     2101 atcttttcca tttttatcaa tcttatggaa ttgagtcacc cagtatttgg tttcccctac
     2161 cgtagattct ggaaattctt gcgatacatg atcgatcaac tcttggtctt tgtctggatc
     2221 aaatgtttgg tcgccattca ctgtaataaa gcgtgtttcg atttttggtt ctttcgcagt
     2281 cactgtttga tctttattgg ttaaatcaaa atgacgagcg aattcttctt cctcttcttt
     2341 gtcgttatag acaatgtgcg ttgcgacaat cgtgtcaccg tctgaaacca tgtctgcttt
     2401 gtagttgaac gtcacatcaa atgtttcttt ttcatcggtc acttttcgtg tttcttttac
     2461 cgtatctaat acagtgactt ttccatcggc agtaatttta tggaattgcg tcacccaata
     2521 tttctctgtc ccaaccgcag atttagggaa tacttgggac actttatcca ctaattcttg
     2581 gtcttttttc gggttgaaat cttttccacc tgtcgttgtc acaaacaaag tagaaatttc
     2641 tggttggtct tcttctggaa ccacgatttc ttcgtttttc acagtaattg gttgtacttc
     2701 tccgtctttg accgttacta aaatcgtttc tgtttgtcct gacgcttgcc cataatgctc
     2761 agggaacaca atttccgtaa atttatacgt accgtctggt aattttagtt tttctggttc
     2821 ttcttttgtt tcatacacaa aggacacatt tttattatgt gcagtcatac cttcaatcag
     2881 gaatttcgct cctggtaatt gtagatgatt gattgaatcg actttattga agtctacttc
     2941 acccgttttt aacaagttgt caatcacatc taattcaaac gtaagcacag gcgtcgtgtt
     3001 atcgccatag gccacagaga acgtggtttg ttcttcagaa ggttgacggt agttgtctgg
     3061 cacatttttt tcaatcaatt ggtatttgtt ttgatcgcca ttgtagaatt ttttgtttgc
     3121 cttccagtgt ccgtctttgt cagtttttac tgaatccaag acttctcctt ttttcacaag
     3181 gactgtttct ttatctggcg cagtaatatc tgttttggcg ataatatcaa attcgatatt
     3241 ttctgcagga acggtatcat aaacaggttt gccttgattc caggattctt ccgcattata
     3301 gacttctttg actttctttc cttctggaat gaactgttgt aagtcatttt ttgcagttaa
     3361 cgtcacttct ttgtttggtt caatcgtaat cgtatgagtt tgtttgtcta gaatataccc
     3421 agctggtgct ttgacttctt tgacatccaa tttattttga acatattttc cgtctttttc
     3481 atagaatgca gggatttctt ttaaggcgat ttcccctttg gcatccgttg tatattgttt
     3541 tgtttctccc gtatcatgac gtttgatttc atacgtcaca tttggaactt ttttccctgt
     3601 gacagcgtct agtttggtta gtttgaagtc gccatttttt agaactttaa tagttagtcc
     3661 agctctatta ggatctggca cataaagctt tcctagattt tgagcaacag gatctacata
     3721 ggcaaaactt gaacctaccc ctctagaatt tggatccagt gaagaaacag aaattcgtcc
     3781 gtcatttgag ttttgatttg cttttataaa caaactattt cctgattttc ttacttgaac
     3841 gttagctgtg ttttcaacaa ccgacccatc ccattgagaa aacacacctt ttgtatcctt
     3901 aacttctact tcttccccta ctttaaccgt cactgtttgg ctatcaaaag aaggatcttc
     3961 aagtaaattg tttatttttc tatctacagt ctgtttgaaa gcttcataat tagattgtcc
     4021 aatagttcca taattaacaa atgaaccacc taaaacttcc caaatatatg tttgtgctaa
     4081 tacatagtta tccattgaac cactagaata gtagccaaaa taaccaatta aagaaatctt
     4141 ttttctaata gcacttgtcg taattaaatt ttcgagtggt gtcacatcat attgaatacc
     4201 cgcactcgtg attgcgcctt cttgaatgca aaaaacatct tctccgtcaa tttgccagcg
     4261 aaccatgtaa tcatgagtat acattgttcc ttcaggagta attcgagtca tattcacatc
     4321 tttacgacca ttttcagtca atgttagatt ggtagcacgt gtctgtgcat tcttttccaa
     4381 gattgcttgc cattcttcag attcttgcgc caatcgttct tgttcagaaa aagaagtcgc
     4441 tttttgaata tcttcaatgg ttataaagta tttctcataa ccttcaggga cctcttgagt
     4501 cattacattg ggcggagaaa catcttctgt cgtgtcctgc gtagttgtct cttctgttgt
     4561 ttcacttgtg gtttcagtac tgatcgcttc ttcactactt tttgattctt cagtcttcat
     4621 tacttcttca gtagaatttg attcatttat cgtttctgca aatgccgtta cagatcctaa
     4681 catgggattg agaaatgtaa cgaaaattgc caaccaagtt gccattcttt taaacatatt
     4741 tttttcgttc ctttcatttt atttttcagt cttctaaagt aatttcttat ggacttcgac
     4801 accgaaacat ttttatcctc ctcaaaacaa aaaaagacta gaacttataa ttctagtctt
     4861 catcaaccac ttacttttct tttattttta gtagaaatct acagtgtgtt tttgtgaccc
     4921 gtcactgtaa ataataacat ccaccataaa acctttgttg taccactgcg aatttggatc
     4981 gctaacctct gcattagccc aatcataggc ttcatctaca gtgttaaata cctttccact
     5041 attaccaatc tctccaacta caattgttgg ctcttcaggt gtcgttgtgc caccatttcc
     5101 gccttgatcg cctccaccag gattgcttgg ttcttcaggc gttgttgttc ctccacctgt
     5161 gcttgggtta gaaggagttg tttctcctcc agtatttcca ccattacctg gttgtgtagg
     5221 tgtttgagta gtgccaccat tgttttctgg tgtacttggc gttgttgtac caccagttgt
     5281 tcctgtttga tctccagctg gtgttgttgg tgtcgttgta tttggtgtcg tttgtgtatt
     5341 tgaagtcgtt ccttgtccta ctggttgatt cggatcaact ttgtctttct cttgaatcac
     5401 tgtttgagcc acagtttcac ctgcgctgtt ttgtacactt gctacattgc cattaaatac
     5461 taatttattt cctgcataga ttaaatccac atttgtaatc ttattcatgt ccgctaattt
     5521 ttggacagta atatttgttg cttgactgat tgcgcttagg gtatctcccc agacaattgt
     5581 gtattcattc cctttaattt ctgctttgat ttgttccgcc gaacgaggtg tccaaacttt
     5641 gttttctgct gcttcagcat tcgttcccac agcaagacta actcctccga ctacgattcc
     5701 tagtgccaat actttcaaca ttttcattgt ttttcgcctc cgatttgaat aaatttattt
     5761 acaaaaacat agtaccaaac ttatgtctat cagtcgatat attttttgta gattttttta
     5821 ttttttgata tactaacttt ggtactaaaa taactaaaag gaatgtcaca catgcaactt
     5881 aataatttaa atgaaataca tgattgggct caaaataatt tacttaatcg taaacaagct
     5941 gccaaaatca ctggacaaaa ctatgatgct ttttctagct ctttacgtag aaataatatc
     6001 aaaccatttc tagaaatagg cgacgaacct ggtccttcca tcattcgctt atatctaaga
     6061 gaagatattg aaaaatacgc aaaacagctc gaagaaaaaa gacagaaacg tcaatgaggc
     6121 gttttctgtc ttggatttac aagtaaaaca ataataaaca cttttacaac ggaggttcta
     6181 tgaataagaa atacattgtt tcagttggtt caatcgtact gattgtactc gctattattg
     6241 ctttttctgt atccaacaaa acagaaacac agccacaaaa tacggttcaa tcgactacta
     6301 caagtcaatc tagcgatcat tccgtctatc cttcactaaa tagtttaaaa gaaacgcatg
     6361 gacaagattt aattgatctt ggttcttctc aaattactac tgtcttattt gaaaagaaaa
     6421 atattgcgtt tattactcct aaagaaggaa ctgaccttga attttctatc aaagtaacaa
     6481 ataccaaaga aaatttattc tccattcctg acattcaata tacccctaac aagttaaaga
     6541 ttggggatac gtttggtttg gctaaagtaa atgaacaata ctttgcttac tcactgaaat
     6601 aagtttctaa tagcaagagg aaaaaccctc ttgttatttt tatcacataa tcattttata
     6661 aaaacgcttc gaaaatatca acatttttga agaaactatt tttctaattt ctcaatttct
     6721 tccaagggat tttcgctaat gacccataca ggcacagatt gctctcgagg ctccccttct
     6781 tctaaccacc aacctgcata atgaacttct tcctcctcag cattaccatg ataccaacca
     6841 acaaaggatt catctacaac gaatgaaatt tcttcttctg gatcaaataa ttcatttttc
     6901 catgcttcaa ttgcttgttc tttggtgtac ttttgtttgt taaatacacc acaaatttcg
     6961 tcatctacat aaaaaatttc aaaatcaaaa ttactcgttc cttttactgg catcttaact
     7021 tttcctcctt tgactcactt caatagatat accactcatg ttcattccat tgtttactag
     7081 ttaattggag ttttatctta gggctttgtg cactctttcc aaaaacttga gcgtcgtttt
     7141 tttcttctaa ccaaaaaagg taatttctta cattcggtac taaatgtttt tcctgcattt
     7201 cttcatgaaa aacatataat tctgcttcat ttttcaacca attcattatt cattccctca
     7261 ttcacttctt cataccgcca ttcagatagt gagatttctt tttgtttctc cacttgtcct
     7321 ataaaaatcg ctgtattcaa caagctgatc acacttaata ataaagcaat gaaactaatg
     7381 gttacactta ctctttcaca ttttgtcatt ttgtttcctt ctttactcgt ttgttatttc
     7441 ttgtaatagg tctaataact ccttcaccga aatttgcaaa tggttcgcaa aaatttcaat
     7501 aaaagcactc gcacacatta ccagaccttc aactgatata tcactgcgac aataaaattc
     7561 tggagaaaca ctgatttctg attctttctt acaattgaac gaaaaaacta tttttgaatc
     7621 aaattccggt aattcttcca caactatgtt ttgggattct accaagaaag ggagtgcctg
     7681 ttctaatgct tcttctggtg tttgagcaga tagaataatt gcttcttgtt cttttatttc
     7741 attgttctca aatactttat aacttttttt cattgttagt tcctctttca ttcctctctc
     7801 aattagctaa gccgttactg gtacgccatt ctctaaattt agtatcgcta atccttaatg
     7861 ctcgtcgaat atcaatcatt cgataaccta ttgcacgaag ttctttatat cttttagcag
     7921 ttagcgtctc taacgtcatt tcaggcaatg ggttcgaatc aaaaatagaa ttcgaaccga
     7981 ttatttgggc caattgttgt acttgtaaca gctcttctga acttaattca gtgattgctc
     8041 gattgcctat cattgcatac attttctgtc gaatatactt cttttcttgt ttagacaaca
     8101 tttctatcca ctcctatttt cattatcatt tgttacctcc acttcagtaa atccacaaat
     8161 aaatgaattc atattccaag taccattccc ttcaactaca acttttctcc tgtcctcttt
     8221 cggatattca agaatcagac cgtttaccaa tatgtttttc actactacta atttgtttgt
     8281 ataccgtgga attgctgttc cgatatactt cactttattt cctacgctaa ttgtcatttc
     8341 ctcactccta ttctttttta tccaaaactt tttcctgtcc tattcctgcc aacgaatata
     8401 cttcactgca tgcttaaaca aaacaaaatg ttgccctgtt ttatttccgt cttttacttg
     8461 ttccacatac agttcatatt tggtcacacc ttttaaaacg ccagatactt tttttcatcc
     8521 attgtcacaa ttagtttttt catcaatcga ttgatactgc actgctcatt tttcttgttc
     8581 atatagtatt cctttctttt tcaaaactag cctcttttgt ttactagttt tcatcaatca
     8641 tcaacgattt aacagatttt ttctttcgca gggtttctag cgtcagcaac cccaaactct
     8701 aaaaaagtgc ctttgctccc ttgaaaaacg agcggacgat tgtactcgtc atcatatgtt
     8761 tttcatacta cctgatccac gcaaataacc tttttttgtt ctgtgccaca cccatccata
     8821 cgtatcgctt gagattcacc atactttttt aggtggttta tccttcctaa gtccctgtta
     8881 gacacgctga ttaacttaga aagtcctgtt tgtatccaat tttcaaagat ctcccaaaag
     8941 aaataagtag gtttacgtgt atcgcttctg ttcaaagtac ctgtttgtac ttgagtattt
     9001 ctactcccct cgttcaaggg attccttcca ccagctagct tttcctttca tcatcaaatg
     9061 ttcctgaaaa gcatttacgt ttcgtgtccg tgtgcttact tattcttttt tcgatagcga
     9121 ttatccgctc gctactcgct attttatact ttgtttgtta gctcaaataa aaactaacaa
     9181 acaaaaaagg ctagtttctt tccgttttat ttttttaact gtcgataaat agttgttagt
     9241 ttaattccaa gttctaaaac caatacgccc aacaaaacca caggaaaaac aattggggca
     9301 aacaagtaaa atcggatagt tttcatgtct tccctctttc tttttggtgt cgctgtggtc
     9361 gttcttgccg ttcaatactt cgatacattt tttattcatt atttgctcgt tttgttttcc
     9421 tctggactgc ttttcttgct ttttgaatac tttatacgct tttcgtgcct gaattaatag
     9481 gtcttgtcgg cgtttcattc gtgagaaaac aatatttgaa accatcaaat cctggttcga
     9541 acaaccacga ttcgcaataa ttttcccatc aaaaaaaata gttttcactt gagaaaaaaa
     9601 cagttggaaa tcttgttttg ttgctaattt ttttgtcaaa atcactgtgc ttttccctgt
     9661 tgaatctaca atgattcttc gaacaatccg attcgataac ttccaacgtt gccgattaaa
     9721 caatgttact tcacaacaaa tatgacgtaa gtgattaggg gtcattttca tgtgtccaat
     9781 tcctccaacc aattatgcat cgaaattttg ggcaattgtg acagcccata aatctgcgtt
     9841 tccaggtctt cataactaca agaacgcttg gcttgaagta atgaatattc tgcttgatct
     9901 gatagttttg ttgccattgg atctagattc aattctctta cacaacattc ccaaaaattc
     9961 aatagcgctc taaaataaaa tccttttcga ttctgaattg cttttccttc atgacgagca
    10021 attttttcat tttgaataca ccgttgatac gttttgctta aatcttctgc aaatgtttcc
    10081 cctaaaattc gatactcgct tccatcagat aaaccatgtg tttgattata ctttcgttgc
    10141 cgcttttcta taattcgttt actgtcgtat atcatgttta gctgctcttt cgcttcacgc
    10201 cattcagctg aactgcgaac aatccatgtc aaaacatctt tcggaacaaa cgtactttcg
    10261 ctgtaatgag caataaactc tttttgccaa tcatcagcca tttcaacagg actagtcgta
    10321 tcgatttttg tatctagatt ttctgtatct agattgttag aagaattatt aaataattgt
    10381 gcagaatttt cacatggatt tttttcgtca aaatcatcga aaatcgctaa attatcaaca
    10441 gattcctcct cttcttttcc ttgtgcagaa tctacactgg ttgagtcaac aggacctaat
    10501 gaatacacat cattttctgt cgtatctaat tctcctagat acaaccgagc tttgtttctt
    10561 tttcctgttt gttgatcaaa tcccatatcc tctatttcta gtagctcata ttctttcaat
    10621 tcttttttga ttgtattaac agaacgtctg caacaattca atgcattggc taattcttgt
    10681 tccgtaaatt caaaataaac aaaaccttga ctatctactt gatttcgttg aatggctaaa
    10741 tctaagcgtg accgaaaaac catatacgcg atttttgctt tatcagacaa tgagcgatat
    10801 ttttcacttg taataaatac ctttggaaat tgatagtata attcattgta tttacgagtt
    10861 gcggtaaatc tttttgttgc catcgttatt acacctcatc ttgtccatat attaagaaag
    10921 aattttggtg ttggtagaca cgaacatctt ccattgtagc aatcaattct tttagataaa
    10981 ttcgaccgaa ttcaattgtt agtaagtcgt aatcttctaa ttcatttttg atttcttcaa
    11041 tttcttcttc agacacattg agtaaaatgg ctaattcttg attggtataa gaaatataga
    11101 catgtttctt ttcatcaatc cgtttttcaa ttaaggattc ttctaagtac tcattcagta
    11161 agctataggc gtattttgct tgtgcagtca tcgcataata ttttgacgat tttataaaga
    11221 atagttttgg catatgaaaa gttgcttgtg tcagcacatc tgttgcgcga aatttgtttt
    11281 ttaccatatt ttttcctcct agaaaaacac gcaaaaaaag ctctgtataa atcgaattac
    11341 acagagtttc ttcttgttga tatttaattt ctaaaatacg tttcgggtaa aaactaaaca
    11401 cttactaata tttagattgt agaatcttcg ccttcattta ttcattttaa agagaaaaag
    11461 atcaagtgaa atgtgcccaa aagtttgatt ctattaattt ttaaacacca ttcataatat
    11521 atttcctata atgtaaaaag cgtcgatact tattaaagca tcgacgttat ttttatagta
    11581 aatttatcaa aatttttgcc aacatttttt ccgatttttt tccctatttc tatctttcct
    11641 tatctttctt agttaatatt atttgatgtt aatatatcac tactaatatt aaaaaacatt
    11701 gatttatcat aggataaaga gtgatagttt aatacttaaa gagacatatt aattttattt
    11761 aaaaattgac attaatgact cttaatcagc gggtcgcggg ttcgagcccc tcacggccca
    11821 ttgggtgcca aacccacgga acaggtaaat gttggtgcga aagcatttcg aaaacattta
    11881 cgctgttctt tttttatttt tcaaaataac tagggctttt tatttttaac tcaaagtcca
    11941 ctgtagccct ttggactttg agttaaaaaa gaagcataat tatgaacagt aaagtttcta
    12001 atataacaat aattcgagaa gctttgcaaa atcatgctgc caatgaactc tctaaacata
    12061 taggattaaa tctaagtact ataaaaggtt aaaaagtgaa gaatatttaa tagaaaaact
    12121 aaatttactt gctgctttcc ccttttccat taatacaaca atcagagagt gtcttttttt
    12181 cgaaaaaatt agaataaata aaagacacgt agttttttta tctacatgtc ttccatcgac
    12241 tacaagttat catttctcaa aacgtattta gcttttaatt gattaatcgt ttcacgttgt
    12301 ctacacattg cctaattaag attttcttat tagaagcatc aatcccgcaa taatacctaa
    12361 aactccagga attacccaca ataatgttaa aagaaaaaat ggaataatag ataaaatgag
    12421 taaactaata ccatagacca ctgttttttg attttttctt actaaaaaac ctattatgaa
    12481 aatcattatc gaaaaaacta gggtaattcc tccaaataaa acaacattcc cgcttagatt
    12541 gactaacata tctatatcct tactggttag ttctgtagta tttaaagtag ttgaatcaat
    12601 agaagataga aacataccac ccatcaatgc taaaagacta cataaaatcc ctgttattcc
    12661 actaattaaa atcaaaataa actctgtttt tctcttattc atttaattct ctccagatta
    12721 agtgttttag aattagaaat tttttgacca tagttaatag cttctaatga acgaataaca
    12781 accactgacg tgttagaaaa ataatcccca atatgttgtt tcttgggcgt gaaagcagcc
    12841 accaaatacc caatttgaaa tacaaaattt tttaatataa acctacatat acactcacga
    12901 ataaagatag tttgccaact tagctctttt tcctcaaagc tcactactct tatcccaaaa
    12961 atcattttcc caattgtttg tcctgaattc agcttagtca aaagtacaaa ataggttaaa
    13021 taaatagcta atgacgctat accataaaaa gtaataatgg atttgtcaga ttccagtccc
    13081 atgattttgt aaaagagacc tatagtagca tgggtgatga tgctaataca aattaaatca
    13141 ataagaaaag caaaaaatcg taaccaaaat cccgcaaata cataattagg caaagtttca
    13201 atgccactct tcttattcat tttatagccc tccatagtaa tacattggtt ttggagtatc
    13261 agaaaatcca agtttttcaa aaactgaaat tacacgatct gatgtagttg cctttagccc
    13321 ttgccattca gcaatcttat tacctaacca agaagaagca aagccagttg tgtcattttt
    13381 atattctata accgtcgcat cagttaattt cttctctttt tttagggaat ctaatgcatc
    13441 ttcagagtaa ccaatttgat ccaccaaccc attttctact gcttggttac catcataaat
    13501 tcttccatcc gcaagttctt tcactgcatt agtatccata tttcttcctt ttgctacaac
    13561 attgacaaaa cgatcataag cactcatcac aaattcctgt ataactttat tctcttcctc
    13621 tgaaggtttg tgttgaggtg acatcatatc ttttaatgca ccacttttat acgtcgcatc
    13681 agttacgcca agtttttcta ataagccaga ataatttaaa ccagaaataa tgacaccaat
    13741 tgatccagtt gttgtttctt ccgtagcaaa tattttatct gaaccagcag aaatgtagta
    13801 ccctccactt gccgctgtat ttttaaatgc agtatagata ggaatatcta gctttttaat
    13861 ttttgccatt tctttagcaa tctcagcact ttcatatatt cctcccccag gtgaatttac
    13921 ttctaataac actcccttaa ccgctttgtc ttcttgaatt ttctttagct gtgttaaaaa
    13981 attctgatgg ttatattgtt ccctactaaa aagattgctt tcacctgtat ctgcaataac
    14041 tccattgacg gatagtttga caatcttttt atttgatgcg ccttcttcta acgtttcttc
    14101 tgtgatttcg ttagatccgt aaaaaatctt atttattcct gttagggttg tatttgatgc
    14161 ttcctcttta tcttttcctg gaattagcgc gacgataatg gatagtaaca ataatccgca
    14221 cgctactccc acagcaatcc atcttctttt attcatataa actcctccta tattcttagt
    14281 aaaaataatt attttttatt tagaaacaca tcgacaattt gttgccaata tgttttttct
    14341 cctacaatta aagacaactc tccagttaat ccataacgaa acggaaacga ttgagttgtt
    14401 tttaacgttc ctttcactgc ataaaaaaca cctttctcag tggcagtaga attttctgaa
    14461 atttcatcta aagttccgtc gatcgtaatc ggagccccac ctttttgatc caatttaaaa
    14521 tgaaccttca tcccttcttt aatataggta ctttcattcg caggtaaaag tgctgtaaat
    14581 ttcatttttg atttttcgtg gatcggataa atttctgcaa gcactgttcc tttggctagt
    14641 tctgcttgtc cctttgtttc ttcatttaaa tgaaccgttc cagatacagg agcttttagt
    14701 attccttttg aaagttgatc gtccaaactt ttgataactg actggttctt ctcttgtttt
    14761 tcatttagct ctgtaactgt ttgttttaca gttgttattg cttgttctag ctcttgcttt
    14821 cctttttccg tttgactaat tctgtcattc tcagaagtag ttggtttaac taatttagct
    14881 ttttctgact gatactgttc tacttctttt tttagttgct caatttgttc atttatgctt
    14941 gctgaaattg tcagtttcac ttgatttttt tgatcatcag aaacattatt tagttgttct
    15001 tgccaattct catagtttgc catcatttct tttgaaaaat cttttaatga ttgattatta
    15061 ctccaagcta cctgtacttg ttgccattct tgtaatttac tatctgaact ctcaatttgt
    15121 ttttctagtt gtctttttgt cttttcgtat acttctaatt gcttttgatc atttaattca
    15181 ctttgcttca aagcgtagcg gagtgattct ttttctgaca acatgctctt taattgatta
    15241 ctgtagccaa aactgtcatc cgttgaaaac aaattttttc ctttacttat actttcaata
    15301 aatagctgag ccatctttat ttgttgagta atacgttggt tttcttgttc tatctgagcc
    15361 ttttcattct ctattaaaga acaatccaat ctaaccaaca catcgtcctt atgaacaaat
    15421 tgattttctt ttaggaagtt ttccattatt ttagcttcta atggcacttg taacttctct
    15481 attttttcgg ggttcaattg agccgttgtc cgaataacaa cctctttttt agcaaaggtt
    15541 aaaaacaatc caataataca taaaaaaaga attacagggt ataacaccca acggtagaag
    15601 ctatgatgtt gtttagtata gatactagaa tgttcgaacc aattattatt ctgcataaat
    15661 tgtttccctc tcctagattt cccataattt ttgatagata ccatcggcaa aacgaagttc
    15721 atcgtgcgtt cctttttcta ccaaatgccc ttcatggaga actaaaattt gatcacatgc
    15781 ttttgctatt ggtaaatgat gcgcaataaa aataatcgtt ttttctttta aattgaggag
    15841 ataattcata attgcatgtt ctaacaaggt atctaatcca ctagttgctt catctaaaat
    15901 taatatgtcc ggatttttta ataaagcccg tgctaaggct aatcgttgtc gttgctctcc
    15961 tgaaagatta tttccacctt cttccaacaa tgtctcgtaa cgtaatggct gttgattaat
    16021 aaattcattt agttggacta tctcacaaac ttctattatt tgttcgaaac ttggtgggct
    16081 atctaaaccg aatataagat tctcaatgat tgttccacta aagaaaaatg actcttgagg
    16141 caaatatgtc actcgatcgc gtaatatctg aaaaggaatg tctaagtgat taatgtttcc
    16201 ataatggatc gatccttctg atgcttcata aaaacgaaca aataactttg caagagttga
    16261 ttttccagaa ccactaactc caactatagc cgttttactg tgcgaaggaa ttgtacatga
    16321 aatttcgttc aacgttgggg ccttcatgct atacgagaat gaaacgtttt ttacagtgat
    16381 atcttgttga aataattgtt cagaaagcat atattcaaaa gactggttcg atttttctga
    16441 atcaatcgcg aaaatttcat ttaaacgatt atttgcaact tgagccgttt gcattttcac
    16501 ttgtaaatta ataatgtttt gtaaaggatc tgtcaaaaat actagtaaag cattgtatgt
    16561 gataagttga cctagactaa ttctattatc cataacaaaa taagaaccta gccataaaat
    16621 taatgcactg ctaattaatt gaatcaaatg tttaattcct tgttgaatat tatcaagcgt
    16681 aaccgtacga aatgattttt tcattaattt aacaaattca tggtctatac ggttatatac
    16741 tttctcttca ccgctatatg ctttaattgt ttctattcct ttaagacttt ctatgatact
    16801 agaatttaca gctgcaccgg ctgccatttc ttccctgttc gatttttcat agcttttcac
    16861 gaaagcgaat attgcaacta cataaaaagg caaagagcat agagtgatta aaaacaacgt
    16921 actattctga atagctaacg taaccccaac aagcaacacc ataccaatat ctaaaaatat
    16981 tgataacgta gcacttgcta aagcatcaat aatcttactg gcatcaagaa aacgagaaat
    17041 aatttcccct gattttctcg tagcaaaaaa attcattggt aaagttaaca catgtttgaa
    17101 atactgaagc ataattgcca tactcattcg ctgtcctaaa ataataagaa gataactacg
    17161 actatattca aacaataccc gaaataaata gacagcgatc aagccaatgg aaattatatt
    17221 taaagttgaa cgtgcttgat tagggatgaa aaaatcaaga ataccttgaa aataatatga
    17281 gcttcctatg ccaaaaaacg taatgaaaat cgatgctaaa acgatgtgga atactaatcc
    17341 tttttgtttc caaattatag gcaaaaaaga acttaatcca gctacttttt ctttaatagg
    17401 aatataagtt tctctaggca taggtaataa aagaatacct gtccattctt tttgaaacgc
    17461 ttcaatcgtt tttctatttt ttccattagc gggatctgca atccatagta cgtttccctt
    17521 cgttttataa attacaacgt agtgcatata gctgttatca accaatatat gtgcaattaa
    17581 tggtaagata agatctcttt ctttccagat ctcactatct gcttgaatag ccatacaatc
    17641 aaaagacaat ttttcaaatg tctttttcaa tccaaatgct gaagtaccct ctaaatcagt
    17701 cccagatagg tcccgtaatt tatgaatcgg gatatcagtt ttaaaatatt ttaaaatcat
    17761 cgaaaggcaa gcaacaccac aatctttttc atcttgttgc tgcacaaaag gaaatgattt
    17821 tatcacttat ctaacacaac tccttctaaa aaatttttat agaaaaattc tcaatatagc
    17881 tttaatacag tgaaaatatt gtctcataat tccacaaaaa ctttgaaaac agttcttgaa
    17941 cactaacttt ttcattcctg aatcaaattt attttggaaa ttttactatt caagaataaa
    18001 cttgataagt ttcaggaaga tttttactgt taatcaaata cgtatgttac agtagcaccg
    18061 taaaaaatat tatttaaaga agcgtgttta aacatgcaaa atgtaaagga actaagtatt
    18121 gtggaaatgc acaaactgtt ggtggaggca ataatgcttg gggaaaattg gggcaagttg
    18181 taggtggact taccacaggg gcagtaggtg gtgcaggttt aggaacagca atttgcggac
    18241 cagcttgcgg tgttgttgga gggttatatg gagctgtcgc tggaggtgct gcagctggct
    18301 gggatgctcg aaaaaaataa aaaaggagaa taagtagtat gcatattaaa aacacaaaaa
    18361 caacatttat attatcttca gaagagttga aaaatattca aggtgggagt gccgtgggag
    18421 ttttaggcac tactttttct ggggcaacag caggagtaaa actttgttca gccggaggac
    18481 cttatgcaat agctgcttgt ggtgtaggtg gagctttatt aggtgctgga ttcagcatgt
    18541 ggaccggtgc gtaaacaatt aaaaaatatc aaacctcaag attggtttgt gtcgattacg
    18601 aacataggta tgttctttat cttttcaaaa gtttggaatg gacagaactt tttactaaag
    18661 cttcttgtgc tagtttttat aattatatta aatttttatc ttttattaaa gtcagataaa
    18721 aattctttgg aaatgtaatg gtagagatat ttttacaaaa ggacttgcag cgctgtagtt
    18781 ggtgctgcag gaggacctat ttcggccgtt acaggaggta ttggtaatgt acttgtcgga
    18841 tgtgcattaa agcctgttcc tgcaagttaa atttataaaa acaggaaagt aattatctca
    18901 aatcctatca agtacaatca taggattgca cttgacagga caaatttttt aactaaaaaa
    18961 tgaaaaacac tttctaaaaa ttagtagaat ttgccaaatc tttcgcatct tccaccatac
    19021 atttaatttt aattcccctt tatggtactt aataaaaaca agaaaagaac tattcatttg
    19081 gccctttttt ggttccagac catagaaata ttcctccagt aactaatatt gctgttatgc
    19141 acgtaactaa taacagctat aaaaattggt acaactttaa taggattcag aaaaatttgc
    19201 actagtgaag gaatctattt tattagaaaa atgcaatctc cctaaagaaa aaaccactaa
    19261 acagtgcttt ctttcaactt ctccttaatt ttttgtgctt tccctaaagt aaagtttctg
    19321 gccaactctg aatcaaaata tagtgaacgt tttttaaaat ctatttcttt aacgttaaag
    19381 atatttacta aacacgctcg actaattctt gttaacatag gatatttctt ttcaagctca
    19441 ctaattcttc cataaaattc gtactgccca tctttagtac acaatgataa tctatgtggt
    19501 aattttgaag cttctacaaa ataaatgtca tttatatcaa aagtaaatgt ttgagaacct
    19561 atggaaaaaa caaatgcttg attcgtaacc tgttttgttg catctatcct ttcttgcgct
    19621 aataacaata actcaacaat ctcgtttcga tactcatcta gtgtttgatc ttttgttaca
    19681 aatcctaacg tttcaactct tctttttatt gttacgggta acatctcatc atgagtagtt
    19741 gtaaaaatga tttttgcttg aacatcatat tttctgatta cttctgctaa ttctataccg
    19801 ttaacttcat gatttaaatc aatatctaag aaatatattc cttgctttgg tcggaattgt
    19861 ttgaggtatt ttttaacttc taacggactt tgtgttttca atactatctt aaatacttta
    19921 tcgtgaaaaa gaatgaaatt atcaataatt cgttctaact gatttaattg tataatttga
    19981 tcttcacata atattattgg ataactcata aatcctcctt ccctcaaaac aacaaaataa
    20041 cttgagtagt aaattgatac ggatcgtttt gatagttcac aaacatattg ggattctttt
    20101 tattgatttc ctctatacta gataaaccta acccttgatg tccctttttt gttgttattc
    20161 cttttttcat tagagtatct atagagagta gtatctctct acaactattc gatatcgaaa
    20221 tttctatctg tttatcatct cggtaaatag ctagagacag ttctctatct tgactctcta
    20281 tagatgcttc aattgcatta tcgagaataa ttccaagcac cctaatacag tcaaatattg
    20341 gaacaggaac ttcaataata gtacttggac attcgaattt acaacaaatc ttattgttat
    20401 ttgcttggta tagcttcgct atcaacaaac ttttcaaata ttcatttttt atatttttta
    20461 aataacgata ttcaaactta ttggttagat aagaattaga atagatctct aattctttta
    20521 tttctttttc caatatcgaa tcatggtttt gattagcgat ttcttttaaa ctcagcaata
    20581 aatttttata gtcatgtcta aatctggcaa atttttcttg atctttttcc agtgaatcag
    20641 tatatttttt tagataaacc aattcttttc ttttcaattg ctgttcatat tgttctcgtt
    20701 gcctaatagt tattctaata aataaaaata gaacaacaaa catttgaatg attaaaaata
    20761 ttaagacacc aaacacaaaa tgatcaaata cttgatattg atgagcaaca taagaaacaa
    20821 ataaaccaat tgtaagtaaa taaatcatga atatagcagt aagtttagaa ctggactgtt
    20881 caacaatcct atttatagct aattttctat ataaccaaat aaacatcagc aaaaacaaag
    20941 aatcaagcat tatctggaat actacataac catacccttt t
//
LOCUS       DAJSBL010000047        20487 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.047, whole genome shotgun sequence.
ACCESSION   DAJSBL010000047 DAJSBL010000000
VERSION     DAJSBL010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20487
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.047"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            3..551
                     /gene="hslV"
                     /locus_tag="ONT85_002029"
     CDS             3..551
                     /gene="hslV"
                     /locus_tag="ONT85_002029"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HCR2378993.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            565..1965
                     /gene="hslU"
                     /locus_tag="ONT85_002030"
     CDS             565..1965
                     /gene="hslU"
                     /locus_tag="ONT85_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002409948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HCR2378994.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            1983..2774
                     /gene="codY"
                     /locus_tag="ONT85_002031"
     CDS             1983..2774
                     /gene="codY"
                     /locus_tag="ONT85_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HCR2378995.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            2856..3731
                     /locus_tag="ONT85_002032"
     CDS             2856..3731
                     /locus_tag="ONT85_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HCR2378996.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(3863..4501)
                     /gene="plsY"
                     /locus_tag="ONT85_002033"
     CDS             complement(3863..4501)
                     /gene="plsY"
                     /locus_tag="ONT85_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HCR2378997.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            4628..5062
                     /locus_tag="ONT85_002034"
     CDS             4628..5062
                     /locus_tag="ONT85_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HCR2378998.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            5228..7273
                     /gene="parE"
                     /locus_tag="ONT85_002035"
     CDS             5228..7273
                     /gene="parE"
                     /locus_tag="ONT85_002035"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HCR2378999.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            7285..9735
                     /gene="parC"
                     /locus_tag="ONT85_002036"
     CDS             7285..9735
                     /gene="parC"
                     /locus_tag="ONT85_002036"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HCR2379000.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            10012..12243
                     /gene="pflB"
                     /locus_tag="ONT85_002037"
     CDS             10012..12243
                     /gene="pflB"
                     /locus_tag="ONT85_002037"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HCR2379001.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            12487..13248
                     /gene="pflA"
                     /locus_tag="ONT85_002038"
     CDS             12487..13248
                     /gene="pflA"
                     /locus_tag="ONT85_002038"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HCR2379002.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            13333..14259
                     /locus_tag="ONT85_002039"
     CDS             13333..14259
                     /locus_tag="ONT85_002039"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HCR2379003.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            14441..15334
                     /locus_tag="ONT85_002040"
     CDS             14441..15334
                     /locus_tag="ONT85_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HCR2379004.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            15468..15593
                     /locus_tag="ONT85_002041"
     CDS             15468..15593
                     /locus_tag="ONT85_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR2379005.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(15727..16359)
                     /locus_tag="ONT85_002042"
     CDS             complement(15727..16359)
                     /locus_tag="ONT85_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2379006.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(16507..17049)
                     /locus_tag="ONT85_002043"
     CDS             complement(16507..17049)
                     /locus_tag="ONT85_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HCR2379007.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            17184..17573
                     /locus_tag="ONT85_002044"
     CDS             17184..17573
                     /locus_tag="ONT85_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2379008.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            17577..18341
                     /locus_tag="ONT85_002045"
     CDS             17577..18341
                     /locus_tag="ONT85_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HCR2379009.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            18470..19417
                     /locus_tag="ONT85_002046"
     CDS             18470..19417
                     /locus_tag="ONT85_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR2379010.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            19547..20287
                     /locus_tag="ONT85_002047"
     CDS             19547..20287
                     /locus_tag="ONT85_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2379011.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
BASE COUNT         7193 a         3481 c         4316 g         5497 t
ORIGIN      
        1 acatggtcga atcaccattt cattcaacca ctatctgtgc cgtcgaaaaa gacgggaaat
       61 ttgctatggc tggagatggc caagtaacaa tgggtgaatc tgttgtcatg aaaggcactg
      121 caaagaaagt acgacgtatc tacaatggag aagttgttgt aggtttcgcc ggaagtgttg
      181 cagatgcgtt tactttagaa gaaaaatttg aaggcaaact aaacgaatat aacgggaatc
      241 tacaacgcgc agctgtcgaa ttagctcaag aatggcggac acagcaatct atgcaaaaac
      301 ttgaagcgat gctgatcgta atgaatgata aagaaatgct tcttgtttca ggtacaggag
      361 aagttatcac accagatgat ggtatcttag ctatcggatc aggagggaat tttgcgttgg
      421 ctgcagcccg agcaatgaaa aactatgctc aaaacgacat gagtgcgaaa gaaattgcag
      481 aaaatgcgtt aaatattgcc gcagatatct gtatctttac taatcataat attatcgtag
      541 aagaactgta agaggtgagt agatatgtat gaattaaaac aaacaccaag acaaatcgta
      601 agtgaattag atgaatatat cgtaggacag cacgcagcaa aaaaatctgt tgccgttgct
      661 ttgcgtaacc gctatcgccg tttgcagtta gatgaaaaaa tgcaacaaga tattacacca
      721 aaaaatatgc tgatgatcgg acctacaggg gtcggtaaaa cagaaatcgc acgacgtttg
      781 gcaaaaatcg tcaatgcacc atttgtcaaa gtcgaagcaa caaaatttac tgaagtcggt
      841 tatgtcggaa gagatgtaga atcaatggtt cgggatttag tagaaaatgc gatccaaatc
      901 gtagaaaaag agcagtacag ccgtgtatac acaagtgcta aaaaaaatgc agaaaaacgt
      961 ttggtaaaaa tcttggtacc tggtatcaaa aaagaacaga aacaatccac taatcaattt
     1021 gaacaaatga tgaatctttt taatgctagt cagcaaaata ccgctgaacc gcaagaagaa
     1081 gtgacagaag atattcgaat caatcgccgt acagtcttgg aacaactaca aaagggttta
     1141 ctggatagtc gtgaagtaac cattgaagtt gaagaccaaa agaaacctgc accaatgatg
     1201 aacaatggat tggagcaaat ggggatcgac ctaaatgaaa ctctaggtgc tttgacaccg
     1261 aagaaaaaag tggaacggac ggtaacagtc aaagaagctt tagagttatt gatcaaggaa
     1321 gaatcagcaa aattagtcaa ggatgctgat atccatagtg aggcaatcaa attagcggaa
     1381 gaaagtggaa ttatttttat cgatgaaatc gataagatca cttctaaatc acagcaatct
     1441 ggagaagtat ccagagaagg cgtccaaaga gacatattgc caattgtcga aggttcacaa
     1501 gtaaacacaa aatacgggat catccaaaca gatcacgtcc tattcattgc ttcaggagct
     1561 ttccatttgt ctaaaccaag tgatctaatt ccagaacttc aaggacgctt cccgatacgg
     1621 gtagaattag atgatttgac agctgaagat tttgtgaaga ttctgaccga accaaataat
     1681 gctttagtaa aacaatacgt cgcgttgatc ggtacagaaa atgtgtcagt tacgtttaca
     1741 aaagaagcaa ttgaacggat cgctcaaatc gctttcgatg tcaaccgtga cacagataat
     1801 atcggcgcac gcagactgca taccattttg gaaaaactac tagaagatct attatttgaa
     1861 gcaccagata tgcaaatggg agaaataacg atcacagaag catatgtgga tgacaaactt
     1921 ggctcaattg cggcaaatga agatttaagt cgctatattt tataaaaaat cggaggacga
     1981 caatgacaga cttgctaacg aaaacccgca agataaacac acttttgcag caaaagaata
     2041 cttttgacat gcaagcagat ttgccttacg ataaaatggc tgtcacacta ggtggtattc
     2101 ttgacagcaa cacctatatc ataggaagtg acggagtatt actaggttat aatgaacgtc
     2161 acgatgtgaa caatgaacga atcaaaacaa tgtttgttga gaaacaattt cctgaatctt
     2221 atactgatac agtggcacag ttgatgaaaa ctgaagctaa tatcccaatt actagtgata
     2281 ttactgcatt tccggtagag tcaagagaga aatatccatt tggtctgacg actgtcgttc
     2341 cgatctttgg agctggcgaa cgtttgggta cgattatctt atctcgtatg gaacaatcgt
     2401 ttgcagatga cgatctagtc ttagcagaat atggagctac ggttgtcggt atgcagatcc
     2461 tttatcagaa gtctcgaagc atcgaagcag atgtacggag tgcaacggct gtccaaatgg
     2521 caatcaacac attatcttat agcgaattga aggctgtaca agctatcttt gaagcattgg
     2581 atggagatga aggtcgtctg actgcttcta atattgccga tagtatcggc atcactcgtt
     2641 ctgtgatcgt caatgcttta cgaaaattgg aatcagctgg aatcattgaa tctcgttcgt
     2701 taggcatgaa gggaacttat ttaaaagtat tgaattcccg ttttaaagac gagttagcaa
     2761 aacacagtta ttagaagtaa ggggactggg gcagatagaa atgtttgttc cagccttccc
     2821 attttaaaaa gtaagtcgaa gttggaggaa tggcaatgac tgttattatt caaaatgacc
     2881 agctgatcgc cgaaatcgct gaacacggtg cggaattgat cagtctgaaa tcaaaagaac
     2941 atggtttaga gtatatctgg caaggtgatc cagcgtattg gggcagacat gcaccggttt
     3001 tattcccttt tgttggacgt ttaaaagatg atcagtatgt ttatcaaggg aaaacatatc
     3061 aaatggggca acacggattt gctcgggata tggattttga agtcattgaa catggacaag
     3121 aaatggctag tttcatctta aggagtacgc cggagacatt agaaaagtat ccatttgatt
     3181 ttgaattagt gatcagctat gaattaggag gagacggcat cacggttcat tatcaagtgg
     3241 caaataccgg tgaaaaagaa atgttctttt ctatcggtgg acatcctgct ttcaatgtac
     3301 ctcttgaaga aggattgtca tttgaagatt tctatctggc attttctcca ttgaaatcaa
     3361 gattacgcct accattgaaa ggcccattca tcgatatgga tcaaaagacg ttaggtcaga
     3421 caaatacgaa tctggctttg aatcgtgaac tttttgagca agatgccttg gtttttgaaa
     3481 caaggggtct aaatgcgtac gccattcgaa gtgaaaaatc ttcccatagc gtaactttaa
     3541 gctataaaga tatgccttat gtagggatct ggtctcctta tccaaaagaa gcgcctttcg
     3601 tttgtatcga accgtggtgc ggaatcgctg acacggtgaa cagtagtgga aacttgatgg
     3661 aaaaagtagg gatcaatcaa ctaggtgccc atgaaatctt caagacaaaa tattccatca
     3721 cagtgaagta agaggttgtg accgagaagt tagcagggaa caccgtttac caaggtgtgt
     3781 aaaaaagcta tcctgtaagt aataagggag tgtgacatcg atttgtcaca ctcccgtttt
     3841 atctctttgg aatgaaaaag tatcaggatt ttttcttgct gctattcaaa ccaaacggga
     3901 ttcgactttc agtatggttt ctgatacgct tgatattgtc tttatgccga taaaagataa
     3961 aggaagaaac taaaaaggca atgatcgtca gtaaccaatt gggcttagcc aaaatcacag
     4021 gagctacata aggaaggacg atcgtagata aagtgatcaa tactgcactg atcatactgc
     4081 tcaaactcac catacttgta caaaataaac taacgataaa gatgatacaa gaataaacaa
     4141 aaaatacagg attaaatcct aaaagcattc ctgcacttgt cgcaacagct tttcctcctt
     4201 tgaacccagc aaaaatcgga aatgtatggc caagaatggc gcatacccca aaccaaagag
     4261 ggttaatccc agaaaggtgg aagatcatcg gcaaacaagt agccaatgta ccttttaaaa
     4321 tatccataag caggacaaca atcccggctg ttttgcctaa tacacgaaat gtattggtcg
     4381 ttccagtatt accgctcccg tgttgtcgga tatctttttt gaaaaacaat ttgccaatcc
     4441 agacaccgga aggaatagaa cctaaaagat atgctaaaat aagtaataag atcaatttca
     4501 ttttttaccc tctttcaagt tgaaaactat cctttatttt agcatgaata ggtaacagga
     4561 acaattgaaa aaatgaaaga tacaagtata atagatggaa taaaaagaaa agcaaggggt
     4621 ggcagaaatg tctgtaaaaa atccaacaca agaaacgatt ttcaactatt tggaagaagc
     4681 aaaaaatatt gccgtcgtag gcttgagcaa taaaccagaa cgaacaagtt acatggttgc
     4741 acaggtattg caagacaatg gatataaaat cataccagta aatccattat tggctggtga
     4801 agaaattcta ggcgagaaag tctatgcgaa tttgacagaa atcgatgatc caatcgatat
     4861 cgtagatgta tttagacgga gtgaattttt acctgaaata gcaggtgatt ttttgaaaac
     4921 tgatgctaaa gtgttctggg cacaattagg gattgaaaac aaagaagcag ctgagatgct
     4981 aagaaaagca ggacgtacag atatcgtaat ggatcgttgt attaagattg aacttggtaa
     5041 gttgaaagaa aaccaaaaat aaaaatgacc acaaagaaga gatgatcgat agaggggaat
     5101 gtcgaaaaag cggttaggag ataattaaaa ttgatttctc gtctaccctc ttttttttca
     5161 cgaccttttc tagtatgata atcgtggtgc ggttttgccg caaagtatta ttgaaggagc
     5221 gtttgccttg gcaaaaaaag taaacaacga atacaatgac tcgtccatcc aagtattgga
     5281 aggattagag gcagttagaa aaagaccagg gatgtacatt ggatccactg acagccgagg
     5341 cttgcatcat ttagtctatg aaatcgtcga taatgcagtt gacgaagcat tatcaggtta
     5401 tggcagtgag attgatgtga ccatccatga agacaatagt ataactgttg cagatagcgg
     5461 acgtgggatg cctgtaggaa tgcatgcttc cggaatacca acagttgaag tgatttttac
     5521 tgtgctgcat gctggcggaa agtttggtca aggagggtac aaaacttctg gtggactgca
     5581 cggtgtagga gccagtgtgg taaatgcctt atcgaaatgg ctaattgtaa cgatcgttag
     5641 agatggcgta gagtatcagc agaaatttaa aaacggcggt aaacctgacg gtactttgaa
     5701 aaagatagga aaaacaaaaa aagcaaacgg gacgactgtc cattttttac cagatgatac
     5761 aatattcagt acaacgaaat tttcatatga aattttagcc gaacggttaa gagaatctgc
     5821 ctttcttcta aaaggcgtta aaatctctct ttctgatctt cgtggagaag aaccagtcaa
     5881 ggaaattttt cattacgaag aaggaatcaa agaatttgtt gattatctga atgaagaaaa
     5941 agatacattg acaccagttg tttatttttc aggagaaaaa gaaggtatcg aagtagaggt
     6001 cgcttatcaa tacaatgacg gttattcaga aaatgtttta tcttttgtca ataacgttcg
     6061 gacaaaagat gggggaaccc atgaagctgg tatgaaagcg gcgatgacga aatcttataa
     6121 tgaatatgcg agaaaagttg gtttgctgaa agaacgggac aagaatctgg aaggtagtga
     6181 tttcagagaa gggttagctg ctgtactttc tatccgtgta cctgaaaatc ttctgcaatt
     6241 tgaagggcaa acaaaagaaa aacttggtac gccagttgct cgtaccgtag tagataacgt
     6301 catcagtgaa caaatgggct tttatttgca ggaaaatagc gaaatgagcc agatgttggt
     6361 ccgtaaagca atcaaagcca gagaagcgag agaagcagca cgtaaagcca gagaagaaag
     6421 ccgaaatggg aagaagcgta aaaaaggcga atctcttctt tcaggaaaat tgacacccgc
     6481 acaatcccga aaccctaaga aaaatgaatt gtatttagtc gaaggggatt ctgctggcgg
     6541 atctgctaaa caaggaagag acagaaagtt ccaagcaatt ctacctttac gaggaaaagt
     6601 catcaatacc gaaaaagcta aaatgcaaga tatcttgaaa aatgaagaaa tcaatacgat
     6661 gatctatacg attggtgcag gagtcggtcc tgagttttct atcgaagact gtaattacga
     6721 caaagtaatc atcatgaccg atgcggatac agacggcgcc catattcaag tattactgct
     6781 tactttcttt tatcgttata tgaaaccgct gattgaagct gggaaagtat atatcgcgtt
     6841 accgccttta tataaagtat ccaaaggaca aggaaagaag caagtcattg aatatgcgtg
     6901 gacagatgac gaattggcag caatgattaa aaaagtcggc aaaggatata tgctgcagcg
     6961 atataaagga cttggagaga tgaatgcgga acaattgtgg gaaacaacga tggatccgac
     7021 ttcccgtacg ttgatccgcg tccgtatcga tgatgctgcg caagctgaac gtcgagtaac
     7081 cacactgatg ggtgacaaag tagaacctcg aagaaaatgg atcgaaaatc acgtgcaatt
     7141 tacgctcgaa gaagatggca gtatcttaga taaaaaagag gatactgaaa tctctccttc
     7201 agtttctaat gatttattag atgaagaacg agcagataaa aatgaaaaca atcagttatt
     7261 tgaagtagaa taggaggtca cctgatggaa aatcgacaag aagtacaaga attaacttta
     7321 gaagaagtga tgggtgaccg cttcggacgt tattccaaat atattatcca agaacgtgca
     7381 ctgcctgata tccgtgatgg attgaagcca gtgcaacgcc ggatattatt tgccatgaac
     7441 aaagatggca atacgtatga caaaggattc cgtaaatcag caaaatctgt cggaaatatc
     7501 atggggaatt atcatcctca tggtgacatc agtatttatg aagcgatggt ccgcatgagc
     7561 caagactgga agttgcggga agtactgatc gaaatgcacg ggaataacgg gagtatggac
     7621 ggtgatccac ctgctgcgat gcgttatact gaagccagat tatccaagtt aagtggagag
     7681 atgctagcag atatcgagaa agaaacagtc gatctcgtgt ggaattttga tgatacagaa
     7741 aaagaaccaa cagtccttcc agctcgatac ccaaaccttc tcgtcaatgg atcgacaggt
     7801 atatctgctg gatatgcaac agaaatcccg actcataact tagctgaagt gatcgatggc
     7861 acaatctata tgatcgatca tccacaagcg agccttgaaa aattgatgga atatattcct
     7921 ggacctgact ttccaacagg agggatcttg caaggaaaag atgaaatcaa aaaagcttat
     7981 gaaacaggac gaggaaaagt tattcttcgt tcgaaaacaa agatagaggc aatgaagggt
     8041 aataaacagc aaatcgtgat cacagaaatc ccatatgaag taaataaagc gaccttggtg
     8101 aaaaaaatgg atgagatccg tttgaataag aaaatcgatg gaatagctga agtacgagat
     8161 gaaagtgata gaacaggtct gcaaattgtc gtcgaattga aaaaagacgc caatgctcaa
     8221 ggaattttga attatttgtt caaaaacact gagctacaga tcaattacaa tttcaatatg
     8281 gtcgctatcg accatatgac tccgcatcaa gttggactga aagacatttt aagaagttat
     8341 atcgagcatc gaaagcaagt cataacaaaa cgtagtcaat tcgatttagc caaagctcaa
     8401 aaaagacagc atatcgtaga aggattgatg aaagctttat cgattctgga tgaagtaatc
     8461 gcaacgattc gtgaaagtaa agacaaaaaa gacgccaaaa agaatttagt cgacgtcttt
     8521 caatttacag aagaacaagc agaagcaatt gtcacattgc aattataccg tttgacgaac
     8581 acggatatca cagagcttca aaaggaatcc gaaagcttga ttgcgcaaat cacagagttg
     8641 aataaaatcc tttcaaatga caaagagctt ttctctgtta tgaaaaaaga attgcgagaa
     8701 gtgaaaaaaa attattcttc cgctcgtcta acgacaatcg aagacgaaat cgaagaaatc
     8761 aaaattgata cgcaagtctt agtggcacaa gaagacgtaa ttgtcagtgt gacgcgagaa
     8821 ggctatgtga agcgaacaag ccttcgttct tatagtgctt ccaaaccaga agaaatcggg
     8881 atgcgagaag gcgactactt gctatactca ggtgaattga gtacattgga ccatgttctt
     8941 ctgatcacaa ataaagcaaa tgtgatctac cgccctgtcc atgaactacc cgatttgaaa
     9001 tggaaagatg ctggtgagca catttctcaa acaatctcca atctgtctgt agacgaatcg
     9061 attttagctg ttttcccata tcaaaaaatt caagcagaga aaacatttgt attcatcagc
     9121 aagaatggat tgatcaagca aacacgaatg acagagttcg aaccatggcg cacctataag
     9181 agccgtccgc tcagctgtat gaagctaaag gcggaagaag atgaactgat tgctgtgtat
     9241 ctgaaagaag atcagccagg cttagatgtt ttcttagtaa cgcatcaagg aatgggtttg
     9301 cgctatcctt taacagatgt tcccgttgtt ggagcaaagg cagctggagt gaagtctatg
     9361 aatttgaaag atggagatag gattatcaac gggctgcttg tgttagcaga aggtgatacg
     9421 ccgatcgtaa tcgttactca gcgaggagct gttaaacgca tgcttgctca agaaatcagc
     9481 caaacttcac gtgcgaagcg gggagtgacg gtcatgcgtg agctgaaaaa aaatccgcac
     9541 cgtattatct atatgtcgga aggaagatca aaagaaataa ccttgatgaa tcaaaaaggg
     9601 cagcagttaa cgattgaccc aactgatttc cctataggtg atcgaacatc gaatggatca
     9661 tttgcgatgg atgagaaaaa aggtggagaa gtcatcgaag ttatcgatgc tcctgagatt
     9721 ttcatcgagt cgtaactgtt gtagtatttt aatgaagaga cgccatctgt attaatacag
     9781 gtggcgtctc tttttcattc atttttaata aatccttata cgtttgcaaa aaaagatgaa
     9841 agtgttcaca atgcttaaat gttggtatgt caacgttgat ttaaatatat gtaaataaaa
     9901 cataaaaatt gttaaataaa aaacaagaaa acccttgcaa agatagggtg ctgtgttata
     9961 ctaatcacgt aaaaactgtt acataaaaca gaaatttagg aggtccatac aatggagcaa
    10021 tggaatggat ttaaaggtac caaatggaaa gaaggcgtag acactagaga ctttattcaa
    10081 aataactaca ctgaatatcg cggtgatgac agctttttgg aaggcccaac agaagcaaca
    10141 gaaaaattat gggatgaatt gcaaaagtta aatgatatcc aattcgagcg taacggtgtc
    10201 tatgatatgg acacagaaat cgtatcaact attacttcac acgatgcagg ctatttggat
    10261 aaagatttgg aaaaaatcgt cggtctacag acagacaaac ctttgaaaca agcattcatg
    10321 ccgttcggcg gaatcaatat ggctaacaat gcgctaaaaa gcaatggcta tacaccaaac
    10381 gatgaattga cacatatttt cactgactgg cgcaaaacac ataatcaagg tgtatttgac
    10441 gcttatactg ctgaaatgcg tgctgctcgt aaaaacaaaa tcattacagg gcttccagac
    10501 gcttacggtc gcggacgtat catcggtgac tatcgtcgtg tggcattgta tggaatcgac
    10561 tatttgatgg aacaaaagaa aaaagatcat gatatgactg gttatcatac aatgagtgaa
    10621 gaagtgattc gtttacgtga agaaatcact gaacaatatc gtgcattagc gcaaatgaaa
    10681 gaaatggctg cgaaatatgg ctatgatatt tctaaaccag ctgcaaatgc aaaagaagct
    10741 gtccaatggc tatacttcgg atatttagca gcaatcaaat cacaaaatgg tgctgcgatg
    10801 tcaatcggac gtatctcagc attccttgat atctatatcc aacgtgattt ggataatgga
    10861 gtaatcacag aaaaagaagc acaagaattg atcgaccact tagtaatgaa attacgtatg
    10921 gtcaaattcg cgcgtacacc tgaatataac caattattct caggattccc tatctgggct
    10981 actttatcca ttgcaggtat gggattagat ggtcgttcat tagttacaaa gaatgatttc
    11041 cgtctgcttc atacattaac aaatatggga ccttcaccag aaccaaactt gactgttttg
    11101 tattctgaac accttccaga aggattcaga acatatgcta gcagaatcgc tatcgaaagt
    11161 tcttcgattc aattcgaaaa tgataatcag cttcgtgaac aatggggttc tgatgattgt
    11221 gcgattgctt gctgtgtatc aggaacagtt gtcggaaaag acatgcaatt ctttggcgct
    11281 cgtgctaact tggctaaaac actattatat gccatcaatg gcggagtcga tgaagtgact
    11341 ggcgcccaag ttggaccaga atatcgtcca atcacatcag aaatactaga ctttgaagaa
    11401 gtgaaacata acttcatgaa tatgatggat tggttagcag aactatatgt caatacgttg
    11461 aacatcattc attatatgca tgataaatat tcttatgaag cagctcagct ggctttgatg
    11521 gattctgact tgcaacgcac atttgctaca ggtattgctg ggatctctca tgcggcagac
    11581 agcttgtcag cgatcaaata tgcaaaagta aaagcaatcc gtaatgaaaa aggaatagca
    11641 gttgattttg aagtggatgg cgacttccct aaatacggaa atgatgatga ccgtgcagat
    11701 gaattagttt catggatctt ggaatacttc atgggacaaa tcaaacgtca taaaacttac
    11761 cgcaactcaa aaccaacaac ttcattattg acgattactt caaatgttgt ttacggtaaa
    11821 aatacaggaa atactcctga tggccgtgaa gctggaaaac cacttgcgcc aggagcaaat
    11881 ccaagttaca atgcggaaca aagtgggtta ttagctagcc tgaactcaac agctaaaatg
    11941 aattacggct gtgcattgga tggaatttca aatacccaaa caatcaatcc ttcaggtctt
    12001 ggaaaagaac aagatacaag aatcgaaaac ttgcgtaatg tcttagatgg atacttctca
    12061 aaaggagctt atcatttgaa tgtcaatgta ttcagtaatg atttattgcg tgatgcaatg
    12121 gaaaatccaa tgaaatatcc gaacttgacg atccgggtat ctggttatgc agttaaattc
    12181 cgtgacttga caagagaaca acaattagat gtcttgatga gaacttcaca cgacagaatg
    12241 taaaaagttc gtgaaaaaag tttttcgact tagcgtcgaa aggacgttga atagccatcg
    12301 catcaaaatc gaataatcag tatgcttatt tgaaagaaac gatttacagg ggcaggactt
    12361 gtatgttccg tccctgtaaa ttttacacat aacgaagcgg caattgcgaa aaattttgtt
    12421 cagaaagctt cgcttcgaca gctaacttgt acttaaaatg aaatcaaaga aaagatgtga
    12481 acttaaatgg aagaaaaaac aatcggctat gttcattcta tcgaaacttt cggttcagta
    12541 gacggacctg gactgcgctt tgtcgtgttt atgcaaggat gccgcatgcg ttgccaattt
    12601 tgtcacaatc cagatacttg gaatattggc ggagggaaag aatacacagc agatgaactt
    12661 ttagataaag cagaacgctt cagaccttac tggggagata aaggtggtat tactgttagt
    12721 ggtggagaac ctctattgca gatcgatttt ttgatcgaac tatttaaaaa agcaaaagag
    12781 cggaagatgc atactacatt ggatacatgc ggaaaacctt ttacgtatga agaacctttc
    12841 ttttcacgtt ttcaagaatt gatgaagtat acagatttac tgttatttga catcaaacac
    12901 atcgataatg aagagcataa aaaactaacg cattgggaca atgaaaatat tttagaaatg
    12961 gctcaatatt tgtcaaaaat caacaaaccg gtttggattc gccatgtact agttcctgag
    13021 cgaagtgatt atgatgaata tttgatccga ctagacaact ttattcaaac cttgtcaaat
    13081 gtagacagag tagagattct tccttatcat acgatgggaa aatacaagtg ggaaacactt
    13141 ggtttgaaat atccattaga agggatcgaa ccacctacaa aagaacgtgt cgaaaatgct
    13201 aaacgattgc ttcatacaga agagtataca aaatatttaa cgcgataggt cgcatcggaa
    13261 agaagataag tattgcttct ttccgttttt ttgctattat taaataggag aaatcacaaa
    13321 ggagcgaatg acatgtcaaa aattttagtt tttggtcacc aaaatccaga tacagatgca
    13381 atcggggcag ccattgcgtt ttcacacttg caaaatgaat taggtaaaga aacagaagca
    13441 gttgctctag gtacacctaa tgaagaaaca caatacgcat tagaccactt tcagctatct
    13501 gcaccacgag tagtagagtc agctaaagca gaaaatgcag atgtcatgct tgtggatcat
    13561 aatgaattcc aacaaagtat ttcagatatc agcgaagtaa atattttagc tgtagtagac
    13621 caccatcgta ttgcaaactt tgaaacagct aatccattgt actatcgtgc agaaccagtt
    13681 ggatgtacaa gtacgattat tttaaaacta tataaagaaa accaagtaac aattcctaaa
    13741 gaaatcgcag gaatcatgct ctcagctatc atttcagata cattgttgtt caaatcacca
    13801 acatgtacgc ctgaagatga agcagctgca aaagaacttg cagcaatcgc tgaagtcgat
    13861 ctaaacactt atggacttga aatgttgaaa gcaggaacaa atctaagtga taaatcagct
    13921 gcggttcttc ttgatttgga tgcaaaaagt ttcccaatgg gcagttccaa cgttcgtatc
    13981 gctcaagtca atacagtgga ccttaatgaa gtgatggatc gacaagcaga gctagaagca
    14041 gcaatggaag atgaaatgaa agcaaatggc tacgatctgt tcgtattagt tgtcacaaac
    14101 atcttagaca gtgattcaga attacttgtt gctggtaaag gaaaagaaaa agtagaagaa
    14161 gctttccaaa caactttgac caacaaccgt gctttgctta aaggtgtcgt ttcacgtaaa
    14221 aaacaagtcg tacctcaatt gacggctgta tttagctaat caaatggtag tatgaagcat
    14281 tcaaaatgaa tggataagcg attgatataa aaagaaacat agaacatact aaaggccgaa
    14341 gaaatggagg agaataacag tcaattatga ctcttgttct tcctcctttt tacgtaaaag
    14401 ccttttaagt aaaataaaac ggatagaaaa gagaacatat atgaactatt attcaaaaaa
    14461 taaggaaaac ttttatcaaa aactgaccga tgatccgctt ttttcttcat tgaccgatta
    14521 tctatatgaa catcgagaac aagaaacgat tctaagagaa ttgaaaaaag aattttcgca
    14581 aaataaattt tcccattttc ttgatttatt gatcgatgca ggattaatca agagggaaga
    14641 acgaagatat catttgaact ttccaatatt tgatcctaaa gattacttgc aacaagccac
    14701 ttcggctgct gaaaccatag ctgaacaatt gaagagactc tctgttgatg aacaaaaatt
    14761 agcgatggga gaagttattt gggcgtattg tttcgaagat gaacggaaag aagcgtattt
    14821 ttatggagta agaaacagcc gagagacaga attgctgagg acaactgcag gaaatgagaa
    14881 gtaccgtttt atcacacttt cctctataga acacttcccg cttactttgg ctaattactt
    14941 ttttgtacag aagaatcagc ttcctgtaac gaaggcattc aaagaattag ccgaacttat
    15001 tggagacgta aatgaatcgt acttttttga tcaaatagaa gttatcgttg acaggatacg
    15061 taagaataaa tataaaaatc gccggcctag tattttccat cagtctctac ttgtgacgaa
    15121 tactataaag gaagaagaat cattcacatt agaattacct attgtcgaaa aaaacaattt
    15181 tgagattgaa ttaccaacat tggatccttc attgacaatg gaggaaatgg cctttcttaa
    15241 acgacaaatc ttttctgagc tgagcaagaa atttataccg catgcatttt cttatatcaa
    15301 agaatatgga acagttttag tgagtaaaac ctaagagagc agtaagcaaa aattatactg
    15361 actcttgcag gaggagcctt tctcttcctt tatggttaaa agaattattt gagtattttg
    15421 cctatctacc acctattaga ttcactacat ggtctggaaa caaaggaatg caaaaaaatg
    15481 gtgagaaatg tggaatgaca aaggaagtag tgataagaaa agttagattt ttaaataatc
    15541 aatattatga ttcagtcaag tatggcattt tatgggaaga actagctgat tagattagga
    15601 agtccatttg ataatccaga atcactaaga aacgaatggc gagtcattaa taatttatcc
    15661 aatcttgggt tattctttta gattgaagtg tttaaaacta aaaaagcatt tatgaagtcg
    15721 taatagtcag gcttcaaaat gctttttatt ttgtttgttt tttctttgcc acataaatac
    15781 acctagactg acgatgaaga gcatatccaa cagatttcct gcgacttgga tcgtcgaagg
    15841 gttggtcagt ggcattaaag caaggacaaa aaaggaaaac aagctgtaaa taaaacaggt
    15901 gacaggaact aatcgaaaga agagcggaca tttcaattgg ctaaaaatga gtgaaaaaat
    15961 aaaataagta aaaatggata aaatcacgac taaaccattc atgataaaga agaaaagcgt
    16021 attgtgatta aacgaataaa aaagaattgc taaaaaagaa aacgttttaa atgcaatcaa
    16081 tacgcttgaa gcttcgttta acgtaccagg atgtcgatga aaaaaatagg caccgaaata
    16141 aacagaaaac agtaagccgc aaagagaaga caaaatcaaa aaaaacggat tttcttcggc
    16201 ttctgtgata tcagaatgat ccaaaagcaa ccagcctaat gcactagaag gcgcatgtga
    16261 ggtcttaagg tatgaataac atagaaaaaa taagatcgtc aaagttgcaa aaaaagagcc
    16321 taaatgtaaa aaattagtgg ttctacgttg aatttccata atatctccaa attaaattga
    16381 taagtatata aagtataata acaaattgat gagtatatgt cgatgttttt taatcaaaat
    16441 aataaataaa aactgtctaa catttaaaat ttaatttaga tattagacag tttttaagtt
    16501 tgtgagttat tttccttgtc gttttctcac ttttttagtc cagaaaccac ctgaaatata
    16561 ttttggctca taagaaatga taaaagcacg ctcttcgatt tctttgatca ggcgatacag
    16621 ttcacgttcg ctttttctag gagaaagtat ttcgagaact agccgctcac cttccagccc
    16681 cattgcagaa gtctgtgtca caccataacc gtgttctctc agtatacccg gcaaactttt
    16741 atcttctgcg ctatttgctg gcaaaatcgt tgtgaccatg atgtatccta atgccaattt
    16801 gtcttcaatc aaaattccga cactgatacc tactgcgtaa cctaacgcat agacaaataa
    16861 attcagtgga ttatctaaac gatctaaaac aagggataag ccgacgatat aaatcgtgat
    16921 ctcgaccata ctgacaagtg gagcaatcag tcgatagcct ttcattgtca acatgaaacg
    16981 taacgtgttc agagtgatat aagcaaaatt aatgaggaag atcatcccca acatttttgg
    17041 atcaatcatt tgtaaaactc ctttcattta tataacaaca atatcaaaaa aaatcgtaag
    17101 tgaagaaaaa ttgaaacttt atacatacaa aaaaatgata aaatgattat aatagaatta
    17161 taaagatagg ttaggtgatt aaaatgaaaa cggtcaatcg tgtgttttgt gccagccacg
    17221 tgctaagtct ccttctcagt atatacttag gtgtctattc gattcaacag ccaattgagg
    17281 tagagaacat tgtttttcag atgtcgctga cagatggttt aattttaagc gtttttttct
    17341 ttttgatact gtttattggt aatattttcg gtagcgttgc cagttttttg aatttttcta
    17401 tttatccact cctttcgcta gcattaggag tggtgggact ttgttctctg tgcttatttc
    17461 ccgagccatt cagtcctgca tttatattat ttggtacact gaacctgttc cagacaactg
    17521 ttggagcatg gttgctatgg cgatcaggaa atttaatgaa aataggtgag taacaattga
    17581 atccaattat attgaaacgt tctgtccatc atattccagt gttagaagtt gttctgaaag
    17641 aattaagaga ggaacgtatt cccgttgtca tctattatca cggatggcag acaagtaagg
    17701 agttagtttt gacgcaagga agaaaaattg cgcagcaggg cattcgtgtg atcctgccag
    17761 atgcgatgaa tcatggggag cgcaaacagc cggtttcttc tattccatcc tatacatttt
    17821 gggatagtat ctacggaaat ctgtttgaat ttgacacact tatcaagcat ttagaaaaaa
    17881 gaggtcttct acatgatcag cttgcagttg gcggggtttc gatgggggga atgacaacat
    17941 gcgccttgtt ggctaaacat cctcatatag tagcgggcgt gtgtttgatg ggttcccctg
    18001 cacctttgga atatggaaaa gaaatcgtaa ggcgcgcatc ggaatataat tatgtcgtac
    18061 caaaagacta ttttgatttg atttcatggg ttaagagcta tgatttgtca ttgcagccgg
    18121 aaaagttagc tggacgtcct ctgttctttt ggcatggaca agctgacgaa aaaatccctt
    18181 atatacaaac ggaacgattt gtagaaaaaa atcggcataa gtcttacgga aaagaaattg
    18241 tttttcgttc ctcaaaaaca cagcgacata tggtacaaat accactaatg gaagaagctt
    18301 ccactttttt agcaaaaaat ttgataaaaa agtataaata attgaaacgg aaaatcactc
    18361 atgacgatag aggattttcc gttttttatt ttgggctata ataaactctg ttaggaaaat
    18421 ttggtataat tgatagtaag aatgacaaca tgttagaaag gaagagagca tgcccacttt
    18481 agcagatgtt gcaaaacgtg ccaatgtttc aaaaatgacg gtctctcgag tcatcaatca
    18541 tccggaacag gtgactgacg aattgaaaga gttagttttc tcagcaatgg ctgaactgga
    18601 ttaccgtcca aatattgctg caaaagcact tgtcacgaat cggtcgcaga tcatcaaatt
    18661 atttatactg gaagaaatcg acacaactga gccctattac atgaatttgc tgatgggaat
    18721 cgctaaacgg atcggtaaag cgcattattc tctccaatta gttacaaatg atgccttcga
    18781 cgtcggttct tgtgatggat acatcatcac aggtgtcaga aaaaaagact tcgattggat
    18841 cgaccgtttg gaaaaaccag ttgtcttgtt tggggaaaat tctgaaggat atgattatgt
    18901 agacagcgat aatgaatatg gtacagcaaa agcaacgcaa tatgccttag aaagagatta
    18961 tcaaacgatt atttatatcg gtatcgaagt ggacgagcct tttgaaattt caagggaaac
    19021 tggttattta tctgtgatga aggaagttga tcttcctagt aaaatcttgc ggtttccaaa
    19081 tcattcgaca gaagtagaaa gatatatgac agaaaactgg gcagatttta cagataaaac
    19141 ttgttttgta tgtagttcag atcggctagc actaggagca gtaagaggga ttataaatgc
    19201 tggaggacac ttgcccgaag aatttggtgt gattggattt gatggggtat ttttagacca
    19261 aatcagttca cctaaattga caacgatcaa gcaagatatt atacgtatgg gtgaagtatg
    19321 tggagaaatg ttgttaaaaa aaatagaaga aaaaggggcg agtcaaggtt atcgtcgttt
    19381 ttttcctgaa ctagttatca gagagacaac gaattaagtc ttattactat atatatattt
    19441 atgaaattca tcgctatttt cttaaaataa tcagcgcatt gctactgaaa acaatgaatc
    19501 tgtgctatta ttgagaaaag ttccattttt gagaaggatg gtttgaatga aagaaatcag
    19561 acgaaaacat ttccgttttc ctgcgtacga tgatgagtta ggcgtaaaat taaccaacga
    19621 caagaagcga gtattatttg acggacaaga agatcttttg actgaacaga atcagtcccc
    19681 gtttgaacag atgaaagaat ttcatttcac gaacacgaac accgagaaaa tccgtcgcaa
    19741 gcgtcgtgca acaaatggat tgaagtcagc tccgcgtaag gaaaatttgg aaaagcataa
    19801 aggaaaatta ccggattata ccggccatta ccaatcagaa atcacatcga caggacgaaa
    19861 aaaacttttt ggtgattcct ctcgttctgt cagacaaagt gaaacaagga cagagtccaa
    19921 gcatactaca cgaaacgcat ctgttgacac gaaggtgcgg gagcgttctt attttgttcc
    19981 taaatatgta cccgcatcga ttatcccaga ccctagagaa agcaaaattt ctgaggatca
    20041 tttaatcgaa tcgatgaaaa aaacggatga ctcttatctg ttgttcgata cagaaccgac
    20101 gccgtatcaa gaaaaaagaa atggtgagcc gtctgtacga acattcaatc gaacaaaaac
    20161 gttagaggaa acaaagcagt cagaagaaaa aaaatcgaaa aaaagccatg gtatcttaga
    20221 acgctcatta caaggattga tcgatgatca aggaaactct ttgggagaaa atagctattt
    20281 taaataagtg atatacaaat ttatattcgg ctacagttgg aaaggaaacg ctgcggcgtt
    20341 tccttatttt atactctatc cgaaagttta tatcgtatcc tcaaattttt ttgagtttaa
    20401 gcgaattttt tctcccaata agtaagaaaa taatggcaaa ataaaaaaac aaactatttt
    20461 cgcagatcgt ttgcaaaaat agtttat
//
LOCUS       DAJSBL010000048        20428 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.048, whole genome shotgun sequence.
ACCESSION   DAJSBL010000048 DAJSBL010000000
VERSION     DAJSBL010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20428
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.048"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            24..1103
                     /locus_tag="ONT85_002048"
     CDS             24..1103
                     /locus_tag="ONT85_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HCR2379012.1"
                     /translation="MTQTHDNTVSRKGKHLSYSERCQIAILKKEGYSNRQVAKVLGRV
                     PQTINNEIDRGTITQLKRQTQNGKVYDTYYSVYDADAGQAFYEKQRLNCGRRPKWANT
                     NAFIDWADDKMLNEKWSPDVVVGFARKHELFDCAIIPGTTALYQWIDRGIMRTKNMDL
                     LEKLSRKIKAVRPGHRPNKRVLGTSIEERPKEVETRETFGHWEIDTVVGNKTKSDAVL
                     LTLAERQTRFEVILKLNGKDTESVDQAVQQLRERAGANFDQLFKTVTSDNGSEFSGLH
                     DALQDVADVYFSHPYASWERGTSENQHKLIRRFVPKGSPISEVSENQILRIQRWMNDY
                     PRKILGYATPHDTFVQAFKQERLVA"
     gene            complement(1300..2001)
                     /locus_tag="ONT85_002049"
                     /pseudo
     CDS             complement(1300..2001)
                     /locus_tag="ONT85_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            complement(2015..2939)
                     /locus_tag="ONT85_002050"
                     /pseudo
     CDS             complement(2015..2939)
                     /locus_tag="ONT85_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            3056..4135
                     /locus_tag="ONT85_002051"
     CDS             3056..4135
                     /locus_tag="ONT85_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HCR2379013.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            4167..4760
                     /locus_tag="ONT85_002052"
     CDS             4167..4760
                     /locus_tag="ONT85_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR2379014.1"
                     /translation="MTKSLQTILESTKEIRVLGIGHCAKDALVLYETHRPDVLLTDIR
                     MPKQTGIDAAKEILAKFPKAILLLLTTFKDEEYIREAFSIGVKGYLIKQNLQAIIPSV
                     KSAYNGQAVFGNEIIETFTQMMKNKPTIDSHAYASFSERELAIIKEVAAGKNNKEIAD
                     ALYLSDGTVRNYISQLLEKLDLRDRTQLAIYFYQHLQ"
     gene            complement(4870..5097)
                     /locus_tag="ONT85_002053"
     CDS             complement(4870..5097)
                     /locus_tag="ONT85_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HCR2379015.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(5200..7788)
                     /locus_tag="ONT85_002054"
     CDS             complement(5200..7788)
                     /locus_tag="ONT85_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HCR2379016.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(7808..8467)
                     /locus_tag="ONT85_002055"
     CDS             complement(7808..8467)
                     /locus_tag="ONT85_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HCR2379017.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVAQHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(8516..9019)
                     /gene="trmL"
                     /locus_tag="ONT85_002056"
     CDS             complement(8516..9019)
                     /gene="trmL"
                     /locus_tag="ONT85_002056"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HCR2379018.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(9240..10106)
                     /locus_tag="ONT85_002057"
     CDS             complement(9240..10106)
                     /locus_tag="ONT85_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HCR2379019.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            10215..10856
                     /locus_tag="ONT85_002058"
     CDS             10215..10856
                     /locus_tag="ONT85_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HCR2379020.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(10918..12279)
                     /gene="mgtE"
                     /locus_tag="ONT85_002059"
     CDS             complement(10918..12279)
                     /gene="mgtE"
                     /locus_tag="ONT85_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HCR2379021.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(12297..13196)
                     /locus_tag="ONT85_002060"
     CDS             complement(12297..13196)
                     /locus_tag="ONT85_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HCR2379022.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(13198..13995)
                     /locus_tag="ONT85_002061"
     CDS             complement(13198..13995)
                     /locus_tag="ONT85_002061"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HCR2379023.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(13973..14677)
                     /locus_tag="ONT85_002062"
     CDS             complement(13973..14677)
                     /locus_tag="ONT85_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HCR2379024.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            14808..15383
                     /locus_tag="ONT85_002063"
     CDS             14808..15383
                     /locus_tag="ONT85_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HCR2379025.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            15501..16178
                     /locus_tag="ONT85_002064"
     CDS             15501..16178
                     /locus_tag="ONT85_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HCR2379026.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
     gene            complement(16257..18065)
                     /gene="pepF"
                     /locus_tag="ONT85_002065"
     CDS             complement(16257..18065)
                     /gene="pepF"
                     /locus_tag="ONT85_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HCR2379027.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(18241..19239)
                     /locus_tag="ONT85_002066"
     CDS             complement(18241..19239)
                     /locus_tag="ONT85_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HCR2379028.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(19465..20118)
                     /locus_tag="ONT85_002067"
     CDS             complement(19465..20118)
                     /locus_tag="ONT85_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HCR2379029.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
BASE COUNT         5913 a         4443 c         3640 g         6432 t
ORIGIN      
        1 tcaatagaca aggatgaatt cacatgacgc aaacccatga taacacagtt tcacgcaagg
       61 gaaaacatct ttcctactca gaacgttgcc aaatcgctat cctgaaaaag gaaggctatt
      121 ccaaccgtca ggttgccaaa gtgctgggac gtgttccgca aaccataaac aatgagattg
      181 atcgcggaac catcacccag ttaaaacgcc aaacgcaaaa tggaaaagtg tatgacacct
      241 actattctgt ttatgacgct gatgcaggac aggccttcta tgaaaagcaa cgactgaact
      301 gtggcagacg ccccaaatgg gcgaacacaa atgccttcat tgattgggca gatgacaaaa
      361 tgttaaatga aaaatggtcg ccggacgtcg tggtcggatt tgccagaaaa cacgagctgt
      421 tcgattgtgc catcattcct ggcacaaccg ctctttatca atggattgac cgcggaatca
      481 tgcgaacaaa gaacatggac ttgcttgaaa aactgtcccg taaaatcaaa gcggtccgcc
      541 caggacaccg tcccaacaag cgtgttctcg ggacgtcaat tgaagaacgc cctaaagaag
      601 ttgaaacacg ggaaacgttc ggtcattggg aaatcgacac ggtagtggga aacaagacca
      661 agtcggatgc cgtgctgctg acgctggctg aacggcagac acgctttgaa gtcatcctaa
      721 agttgaacgg gaaggacacc gaatcggtgg accaagcggt tcaacagcta agagaacggg
      781 ctggcgctaa cttcgatcaa ttatttaaga ccgttacatc cgacaatggc tcggagtttt
      841 ctgggctaca cgatgctctg caagatgtag ctgacgtcta tttcagccac ccctatgctt
      901 cttgggagcg tgggacgagc gaaaaccagc acaaattaat tcgccgtttc gtccccaagg
      961 gaagcccgat tagtgaggtg agcgagaacc agattttgcg gatccagcgt tggatgaacg
     1021 attacccgcg gaaaattttg ggttatgcga caccacatga tacattcgtc caggcattca
     1081 agcaagaacg gttggtggct tgacccatta ttccttgata acgatcctca ctcgggacat
     1141 ggcttcatgt ctggaaagct ggcctggggc cagaatcgtc tgaagactat catacctcag
     1201 ttcattatca aggcctcact gatttagaat aacgaggcag ttacggtatc aaacaaatga
     1261 gttacctgta gtggctaact taaacttgaa atttacggac gtaaagcttg ctgccaaatt
     1321 agcgattgaa actaacgatt gctttctagg aatcaaggac aagcgaatac catttgacga
     1381 ttgatttgct tgctgatcct gtacattgcg gaccccccac atcgtcccgt agagaatcgc
     1441 cattccaatc agagtgaaga aataaaaact tttgaaactc atattacgac ctgcatgaac
     1501 ttcttgaaaa gatacttctt gttcaaaaac agtcatgatg ggcgcgagat caacgttctc
     1561 cacttctgcc actttactcg aaattatacc aatacgctgt aaaaattgat ccatgacgtt
     1621 tttcatcaac gtctgactca ttcctgcttg acccacatgg agcgaaacag atgaaccatc
     1681 aatgagataa aaaccagcca aatcatctgc atttagcttt tcattcgctt ctacttccga
     1741 tagttcctgt acttcaaata atggtttatc attgacttcg acttgttcta agactgtggt
     1801 aaaattttcc gaaagcgctg tttgatccgc tttgattaca attccagtat taatcgtttc
     1861 gagattgcca ccttgatcaa tctcgccaaa ggccgccata aacatcaatc ctaaaagaat
     1921 tggaaaggcc aatgtccaaa acaacatgga tttatttcgc agcagtattt ttgtgcgata
     1981 tttataaaaa tgccaaaaca ttatttcccc tcctctagtc tctcaaatcc ttgccagtaa
     2041 tctctaaaaa gacatcattc aaggacgctt ctttggttgc tatttttccg taactgatct
     2101 tttcttcttg taagattgta agaatcggca acaatactgt atttagttca gtagttgtta
     2161 cttggagctg acctttttta taagcgattc catcagaagc tattgttcgc tgacaacacg
     2221 ttttctggga gtaatgggat gtcgatcgtc aaacgatccg ccgtccgtac catatctttt
     2281 agagattctt tggttccttt agcaatcacc tgtccttgat ccatgatcac gatctggtcg
     2341 caaagaattt ctacttcttc catgtaatgt gttgtataga caatggttgc tccttgtcga
     2401 ttcagctcct tgatactttc taaaatttta ttccgacttt gggggtctac agctactgtc
     2461 ggctcatcta aaaaaattaa ccgcggcttg tgggcaatcc cacatgcgat gttcaaccga
     2521 cgtttcaagc caccactaag ttctcctgga cgaaacgaaa catatttttc taatcccact
     2581 aattgaatca cctcttcaat caaggctttc cttttctctt tttcggaaac atataaaccg
     2641 caaaaataat cgatattttc ttgcacactt agttcgtcaa agaccgcaac gtcttgagga
     2701 acgaccccaa tcattgcctt gacagcataa ttttcaggag tcattgttgt cccgaacaat
     2761 ttaatggttc ctttgtcata atttaacaaa gaaagcatac agttgatggc agtcgatttc
     2821 ccactgccgt ttggtcctaa cagacctaag acttctcctt cttgtacttg cagatcaaaa
     2881 tgattcaatg ctacaaaatc tccatagcgt ttgactaatt tatcaatttc gataatcatc
     2941 atgaaaaact ccttccaaat ttcttgttga tctgactata ataaagtgca agaaaagaaa
     3001 ctagtgacga ttggctaaac cacacatgac atttgtcaca atgaaggagt ctgctatgca
     3061 taaatctcgg ctactcaacg aaactagctt tctactggtt atctcattgc ttttgctggt
     3121 aagtcaagga ttcgttgcta tgcaagttat ctatttattg atcgccgttt tattcactgg
     3181 tgtcagttat ttactgccta caaagccaaa ttggattttc caagttattc tatttctttt
     3241 atgtgccttt ttacctgatc ttgtcctgtt tctgccagcg acgatgcgag tgatcgcccc
     3301 tgaagaaagc caccttgatt acttgttttt gttaggtttt ttgttgattt cgacggttgg
     3361 taatcatccc cccatcaagg tcgcctttgt cgtaattttg ctgttttttt ccttttactt
     3421 acgaaaaaaa gatcaacgaa cgaccgatct tgagaaaaag tacattgtct ccaaagatac
     3481 tcattgggaa aaacaacaag agttatcgga acaaaataaa gcattggccc tgtcacaaga
     3541 aacacaatta gccttgcaaa ttgtggaaga acggaaccga attgcccgag acatccatga
     3601 caatgtcggt catctgctgt caagtgcgat tttacaaatt ggtgcgcttg aaatgatcaa
     3661 tcaagaacca aaactaaaag aaccattaga aaatttaagt gccaccgttc acactgggat
     3721 ggatcggatt cgtcaaagtg tccatgacct ccatgagaca tcgatctctt ttcaacactc
     3781 cttagagttt ctgatcgctg attttccttc ttctgttacg attgaaggaa acttgtttga
     3841 aggattgaat gaaacgcagc aaaactgctt cttaatggtc atcaaagaag ccttaaccaa
     3901 cagtatcaaa catagtcatg ccaaaaccgt tgttttacat tttcgaacgt tgccggcctt
     3961 ttatcgcttg caaatcacga atgatggcac cagcccacaa aaaaattctg ctcccggtat
     4021 tggtttgaaa acgatgcgtc aacgtgttca agaacaaaag ggacaagtcc atattttcca
     4081 agaaaaccag caatttcagt taacgatcat tttaccaagg gagggacacc aatgataaca
     4141 gtagtcatta ttgagatgat ccatttgtga cgaaatcgtt acaaaccatt ttagaaagca
     4201 cgaaagagat ccgtgtgtta ggcattggtc attgtgccaa ggatgcgcta gtcttatacg
     4261 aaacccatcg tcccgatgtt ttgctgacgg acatccgcat gcctaaacaa acgggcatcg
     4321 atgctgccaa agaaattttg gctaaatttc ccaaagccat ccttttgctt ttgacgacat
     4381 tcaaagatga agaatacatc cgcgaagcat tttccatcgg tgtcaaaggg tatttaatca
     4441 aacagaactt gcaagcgatt attccttctg tcaaatccgc ttataatgga caggccgtat
     4501 ttggtaatga aatcatcgag actttcactc aaatgatgaa gaataaaccg acgattgata
     4561 gtcatgctta tgcttctttt agtgaacgtg agcttgcaat catcaaagaa gtagctgccg
     4621 ggaaaaacaa taaagaaatt gcagatgcct tgtacttgag tgacggcact gttcgcaatt
     4681 acatcagcca attactagaa aagctagatt tacgggatcg cacgcaattg gctatctatt
     4741 tttatcagca tttgcaataa gtcttatcta acacaaaaaa acctgaatga tgctccttcg
     4801 catcattcag gtttttttca catcttctat tcaattgtcc cgcaaaccat ttcctcacga
     4861 ttgaaagaat caaggattcg cagggctgcc aggattcgtt ggattgttcg gcgtgttagg
     4921 cggaaaaata ggcgccttgt ctaacgtttt ttctagtttt gattcttcac gatcgtctcg
     4981 ttctacatca ggactttcat tcaatttgaa ttctttttcc atctcatcta actttttaaa
     5041 gtcattgcca gattcttttg ctaatttcgc taatttatct tcggtttctt tagccataaa
     5101 gtaacgcccc ccttttgatt tcagcttagc atatcacact gaaaaaacaa aagaaaacgc
     5161 taaccaacga tcatttttag ctatttctca tctttaaaat caagcatttt ttgccggtat
     5221 aaaatcgtag ggagaactgt tttccatacc aatcatcgga tctactaact ggttatcgat
     5281 cacttctgct gtaagaacga aatctttaga aacagcaggt tctacctctt ctttcttttc
     5341 ttctccaaca aatgaagaaa acagatctgg ttccactgtt tccattactt ggtttttcgt
     5401 ttcattcgtc ccactgtcat cttctacagg tagcttttct tttacaaccc gtggctcttt
     5461 tgtatagttc ccatcttcaa atggatcatt tccgaccaaa gaatcttcat aagctgctag
     5521 tttggtccgg agagtgatat tcattttatc attatccatg atccttgatt tcaacgtagt
     5581 catccggctt gctgattcat tcgctacaca agtgacatag gcttgctctt cccctaacaa
     5641 aatcaacagt tccttactgc gagtcactgc cgtgtagagc aaattacgct gaagcatccg
     5701 ctgatattga tggaccatgg gcaaaataac catcttgaat tcactgcctt gtgatttatg
     5761 gattgagcaa caataagaaa gagtgatttt attccattcg tttcttttat aagtgacctc
     5821 atttgcatca aactggataa ctaattcatc cactttatct tcagaatctt ttgccaagat
     5881 gatacccgtg atttctccca tatctccatt gaatacattc aattcgggag tattgactaa
     5941 ttggaggact ttgtctccta tccgataaac ggtgtcgttc caatgtactt ccttttttcc
     6001 aggtttccct ggattgaata tctcctgcat cattttattt aacgcatcga tccctgcagg
     6061 tccccgatac attggtgcta atacttggat atcttgtgca cggaatccct tatttttcgc
     6121 tttttccaca attttagaga tcaagggttc aatttgataa gcatcacttc ggaaatagga
     6181 acgatctttt tgattctttg tgaaatcttt tggcagacgt ccttctttga tctcatgggc
     6241 aagaggaata atactagaac catcaccttg ccgataaatc tgattgagtt ccattttggg
     6301 aatttgttcg atttccaaaa gatcatggag tacttgacca ggaccgacag agggaagctg
     6361 atctttgtct cctactaaga taacttgcat attcgtcgga atcgctttga ataatgtgtt
     6421 agccagccat gtatcgacca tagacatctc atcaacaatc aaaagtccgc cttctaattc
     6481 tttcgtactg actgaaggtg ttttttcccg tccattcagc cccagaagac gatggatcgt
     6541 actggcaggc agacctgtcg tttcgttcat tcgtttagct gctcggccag ttggtgctgc
     6601 aagtagaata ggaaaaattt cttgggtata ttttgaagga tccaattcca gtccgttcaa
     6661 ctctgcaaaa agattgacga ttccgttgat cacagtagtt ttccccgtac ctggtcctcc
     6721 tgttaaaata aatagagggg aacggattgc ttcagcgata gcttgttctt gcgaaggtcc
     6781 atactgtatg ccgaatattt tttcgatacg ccgaatattc ttctgcactt ctttttcagg
     6841 ataatggatt tcttttttac gactgagaag gcgctggatc gaattaccga tcccccattc
     6901 agaaaagtac aaactgtttt cgtaaagatt agtctcttct tgttgtatct ttccttcttc
     6961 aacaagttgg ataattacag tagcaacctg atcaggctgg atttcaattg ggcgacttgt
     7021 ttctaaaata cggatcgttt gctgaagcaa tgtctccgcc ggtacgtatg tatctcctgt
     7081 ttgaactgca tgctgaaaaa tctgatgcaa gatagctgct cgaattcttc tcggtgaatc
     7141 tgctgcaata ccgatttgtt cggctagatt atctgcacgt ttgaacccga ttccttctat
     7201 gtcttcgact agctggtacg gattttcttc gatgatatcc aaagactcgt tcttataggc
     7261 ctggtagata ctgaaagcta actgactacc gaacccgtaa cgattcagtc ccatgatcac
     7321 ttgatccatc ccgtggttta gccgaatcgt ctcagcaagc atttctcttt ttggcttcgt
     7381 caaaccggag atttgtttta ataccgaagg atcagcaatg attttttcga tagcatcttc
     7441 tcccagcaat tctacaattt tttctgctgt tttcttcccg attcctggga atttttcgct
     7501 ggaaagatac gctacgatcc catttgcagt tgttggcttt tcttgctcat atctttcgag
     7561 tttaagctgc cttccgtatt taggatgatc cactaattct ccataaaaac gataagtctc
     7621 attttcttga atatcgccga aacttccggt gactacaatc tctttttcac ggtaatcagc
     7681 tgtggtttca ctgatgtgta ccaaaagcac tttgtaaaaa ttactaggat tttgaaaaaa
     7741 aatcgcggca actgtaccaa cgaaataagg catttcttct ggctgcatcg gaagccgcct
     7801 ccttcttcta tagtaaaggt tctggtgctg tttcagttcc taaaaaatct gtttggttcc
     7861 atactttcag ttcatatggc aataatctgc aaggctcttc agtctctaaa atcgtcaaac
     7921 tactattgaa caatccaccc atttcacgca actcacttag ctcttttcct gtcatccatt
     7981 gaatggctgc agttaaagaa gctccatgtc ctacgaataa aagtgggcca tctttatgtt
     8041 gggcaaccgc atctgctaca acagtttcga tacgtgtgat tgccgattca atcgtttctc
     8101 cgtgaaaagg agaaggatca tacagatcta aatgattacg caaattcggc agattatccg
     8161 gatatttctg gtacatttca tcgatcgact gaccttctaa agtacccagc cctagttctc
     8221 ttagcttatc tgtataaaaa atctctaccg gacgagcaag ctctttgttg ataccttctg
     8281 ctgtatcacg tgctcgcttt gctgtactgg aataaattgc tttaaaagga acatatttta
     8341 cctttttccc aaatgctttg atttcttcgt aactttgtgg caaaagcgga gaatcgccat
     8401 tgctcccttg aaaacgtctt tcctgattcc attgtgtctt cccatgtcgg gtaaaatata
     8461 gttccatacg cagcaaactc ctgtctttct tttattttac tctataataa actgtctaat
     8521 ccagcttgtc attttcatag tgatgcgtca attctaaatt agggaatcct tgctggcgaa
     8581 gtgcttcata cactatcatt gcagctgtat tcgataaatt caatgagcgg acatgctcat
     8641 cattcatagg gatgcgtaaa cacttttctt cgttttcacg cataaatgtt tctggcaaac
     8701 cggttgtttc ttttccaaat aaaaagaagt gctcttctcc atcagcaaaa ttttcgtcac
     8761 tatatacttt atgggcaaat tttgagatca aatgtagttt acgattacct aaaaatgtta
     8821 aaaaagcttc taaattactg tggtaagtaa tatttacatc attccaataa tccaaccctg
     8881 cccgttttag atgtttgtca tcagttgaaa aacctagtgg ctcgattaaa tgaagcggag
     8941 tattcgtggc tgcacacgta cgggcaatat ttccagtgtt tgcagggatt aatggttcaa
     9001 ataaaacgat atgattcatc aggatcattc ctctcatatt tatgttcgtt tctattttct
     9061 gacaatttga tttttgcata aaaaaaaacg tatcaactcg gtgtcagtca ctgcgagtcg
     9121 ttacgttagt gaagctcttc tcactaactt ttatcagaac aggaacctga taaaaaccaa
     9181 tgaaaaaaca acttgttgct aatataacac cctcaacttt tttttgcaac ctctactcat
     9241 taaaaactgc tagaaaaacg ttttctctta gttcctttta ataattctat gtcaattgta
     9301 atcttcttca aaggattctt atataagtct gctttgattc gctcttctac ctgccattct
     9361 aaaggtgaca tggccaaaag atctttttga ttttcctctg gtatctcaaa ttcataggtt
     9421 acacgctgac gttcagttaa tttcattgct tcagcaaact tggctactac acgttcattg
     9481 gaataactct gtttttcagg ttgattcgga taaaaagcct gacgtagttc ctttaaataa
     9541 ttttcttgtg gcaccacctt caacacttct ccatcttctg agagtacacg ttggaattct
     9601 tggtaatgag aaggcgaaaa gatatttaaa accgcgttga atgtttgatc agcaaacggc
     9661 aaattggtca aatcagctac acaccaaaat gcttcggcag attgttctgt agctgcgtag
     9721 actccttctt ttgaaatatc aaatccaaca gcattttgaa tctttttcaa ttttgaaagt
     9781 tcattcaaga agctaccttc tccgcagcca acatctaata gcgagacttc ttcaggtaaa
     9841 tgctgcgcaa tagtctcaat aacgggctga tacatgccag attggatcat tctacgcctt
     9901 ggaacgaaca ttccttgatc gtattctgtt ttaaccgcat gggataaaaa ataaagtgtt
     9961 ccttttttgg aaaggtcaaa acggtgatta tttttgcata tcaagccatt atcagacttt
    10021 tttacttctt cttggcaaac cggacagcgg aacagctgat gattttctgc caaaaactgg
    10081 cgtcctcggt caattttttt taacatagtt ccccctcatt tctgttttat cttaccgtta
    10141 gatgctcact ttggcaactg ctaaaaatat gatacaatgc ctagaagaaa gtgaaagata
    10201 aaggagaaga ttagatgatc aaagaatttt gcgctgaaaa ctacacatct attcctctag
    10261 caattgctag aggagccgat cggatcgaat tgtgtgacaa cttagcggtc ggtggcacaa
    10321 ccccgagtac tggtgtgatc gaagaaacgt taagttatgc tggggaaaaa gatatccccg
    10381 taatgacgat tattcgtcct agagggggcg acttcgtcta taacgatatc gaattgaaaa
    10441 tcatgcatac tgatttgatt gaagcaaaga aattagggac ggatggcgta gtatttggtt
    10501 gtcttactcc ttcaggctgg ctagacgaag aggccttaga actattgatc gaaacagcag
    10561 aagggttgca gatcaccttc catatggctt ttgacagtat tcctgaagat cggcaattcg
    10621 aagcaatcga ttggctggta gaacatggtg ttgaccgtat cttgactcat ggcggtcctg
    10681 ctgacaaaac tattgtagaa aatttcccac ggcttaaaga attgattgcc tatacagatg
    10741 gccgcttaat cattttgcct ggtggtggtg tcactgcaga gaatgcagca catgtagcta
    10801 aagaattgaa cgtaagcgaa gtacacggaa ccaaaatcgt ggatcttcag ttataatctt
    10861 ataaacaaaa aaacatttgc aacaggcata atgcttattg caaatgtttt tgttttttta
    10921 cataaaatag cccataaata aagaagcaat attgaaataa atcagtacac tcgtcaaatc
    10981 gctcaaagtc gtgataaagg gaccgcttgc tactgctgga tcaaatccta gacgatccat
    11041 tagcatcggg attaaactac ctgcaagatt cgccactgtg attgcaaata gcatagccat
    11101 ccctatgaca aaccccaaga taaaattatg tttccaaaaa cctacgatta gaaaaatcgt
    11161 gatccccgta acagctcctg tgacaacgcc ggtcaagact tcagccaaaa caagtcgaac
    11221 aaatccatga tctttctcat cagaaatcgc aagtcgacga acagccacag ctagcgactg
    11281 ggttccagca ttaccggcag ttcctgtaat cagcgaaatg aagactgcta aaatactcgc
    11341 ttcacttacc aagtcttcat agtgactgat taaagaagcg gtcgacatac ccaaaaacaa
    11401 cagagtgatc agccaaggca aacgtcgaga cgctgccttt actggatttt cattaatttc
    11461 ttccacattg accccggcaa gtcctgaata atcgctagca gcttcatcat caataacgtc
    11521 gatgatatca tcgactgtga cgattcctaa caaatgatca tcataatcgg taacaggaag
    11581 agctaagaag tcataatctc taaatgtttg tgccacatct tcttggtcgt cgcctacatg
    11641 aactgaaagc actcgttcgc tcatcagatc agaaatcatt gcgtcgtcat cattcacgat
    11701 taggtcgcgc agagaaataa cccctactag ctgaccttct tgattaacga catagatgta
    11761 atagattgtt tcagctacat ctgcttcgtt cttcagcacg tacatcgcag agcgaacagt
    11821 ttgattggct acgatcgaaa caaattcagt tgtcatgatc gcgccagccg tttcatcctc
    11881 gtaatggaga agttccttga tttcactggc atcgtctgcc gacatcaaac ttaaatattt
    11941 agcaatttgc tttttatcca gcgtattaag caagtcaaca gcattatctg tatacatctc
    12001 agaaagcata tcagctgcat agctaggacg catttcatct aggtattctt tcatatgttc
    12061 gttgtcttct tcaatcacat cgaacatatc cgctaattct ttaggcgaca gataggaata
    12121 gatcagctgc ctgttctctt ctgtaagaga ttggtagaat tgcccctgct catagatatg
    12181 catctctaaa aaactgtttc tgaatgcctg aatattattt tcccttaaat cttgagtcaa
    12241 ctgggcaaat ctttcctcta attcttgatt ttcatccaaa ccaatctcac ctcgccttat
    12301 tcttcaaacc gtctcacaac tgcctccatg tctgcggcta aaggctgctt gaactccagc
    12361 aactcttctg taaatggatg gtaaaaacgc agatcataac aatgaagggc ttgtcttttt
    12421 attcccagat ccattcgccc gccatacata tcatcaccga gcagggcaca gccaattgcg
    12481 gaaaaatgaa ctcttatttg atgtgtcctt cctgtatgca gctggatatc aactaacgcg
    12541 atttctgtta gtcgcttctc tatccagtac tcagtctgtg ccgaacgtcc ttcatcagag
    12601 accattcgct tcaatagcga agaataatct cgggcgatcg gcaaatcgat atgtccatgt
    12661 gccgataaat ctgaaagcct tccactgact aaggcctgat attttttcac aaacttcttt
    12721 tcacgaagtt gcatatctaa ttttgcgtgc gcaaatccat gctttgcaaa aagcatcaaa
    12781 ccagatgtat cccgatctag tcgcgtcacc acatggatta cttggttttc gtaaccttgt
    12841 tttttataat aagcctttac acgattagcc attgtcccat ttggatggta ttgggcagga
    12901 atagacgaaa cgcctgctgg tttatttact accagaacgt gttcatcttc atacacgata
    12961 tcgatcggtg tttcatccaa aagtacggtt tcatgttctc cttcagcagg aatcacaatg
    13021 gttaccttat catccttagc tagagaaaac aagacatttt ctacttgatc attaactttg
    13081 atttgaccgc cttgaaattt tattttcgct aacagaccct ttgagatccc tttttctttt
    13141 aaaaaatatt ttacttgctg agaatgatct ttctcataat cccatgtaaa ctgcatatca
    13201 atcctcacta ataaaggcat ctttcacacg atgccaaaaa tgcatatgtc gataagaagc
    13261 aaaatggatc cgttcttctg ctattcgata ataaattgag cggatatttg cttttgcgac
    13321 attcaattga tcaaccgtta caagataatc ttctgtattt tccaatttta tttctagcca
    13381 ttcatcatgg gcaatcacga tcggagaacc caaagtacga aacactcgat tattcagtga
    13441 agcaatctct gccaactgaa aggcattgat actaggatgg atcacagcac cgccgacact
    13501 tttattatat gctgtcgaac cagtaggcgt agaaatcgtc aatccatctc ctctaaaact
    13561 ttcaaacagc tcgtttttga tgtatacatc ggctaccatc gtacgattgg cccgtttaat
    13621 cgttgattca ttcagtgcta aaaaatgctg gtccggtgtt tcatccaaat aactgatccg
    13681 aacatccagc aagggataac taacactttg ttcccggttc gttcgtaagc tgtctactag
    13741 ttcttccagc tcgtaatccc gccaatccgt gtaaaaccct aaatgacctg tatgcacgcc
    13801 caagaaacgc acatcattca gacaatgact gtagtgatgg aacgccgaca ataatgtgcc
    13861 gtctccgccg actgagatga ccaattctgg ctcatgatca tctcgttgaa tccctgcttg
    13921 ttctaaaagt gcgttcaaac gttctgtcac ttctatagtc tttgcttctt tattatgaac
    13981 gatcgctact ttcatcccct gcctcctctc tttcgctttc tttcttcaaa agcgttcgat
    14041 agtagacatc atctgatttt cctcttccat gggagaataa ccgctgtgct tcttggattt
    14101 cttcacggat actggacatc tcttcatcca actgataggc agcttctgcc gcacgctgaa
    14161 gtcgttcact catttcttct ggaaaaacgc cttgatattt ataattcagc gaatgctcaa
    14221 tcgttgccca gaaattcatc gcaagagtcc gaatctggat ttccgccaaa attttcttct
    14281 caccgctgat cagctgtaca ggatactcga tcaccagatg ataagaacgg tagccacttg
    14341 cttttttatt tgtgatgtaa tctcgttctt ggagaacttt catgtctttt cttttacgga
    14401 tgatctcaac aacttgatgg atatcttcta caaactgaca catgatcctt aacccggcaa
    14461 tatcagacat ttcttcttct aaacgtccca ttggaatatg ccgtaattgt gattttgtta
    14521 caatactatc gactggcttg actctcccag tgacaaattc aatcgggaca tgtcgatttt
    14581 gttcacggaa ctgtttgcgg atcccccgca gtttcacttt caattctgat acagtttgtt
    14641 catatggtgc taaaaatgct tcccaatctc gttccatagc tgatacctct acttcattaa
    14701 cattcattgt ttattctacc atatatgcaa agttttttcg tactcttgat ttatgcattg
    14761 accaaaaaaa gaaaattagg caaaatagac aaaaaggagt cgatcaaatg acacaaatgc
    14821 ttgaaatcga atataaatct atgctgacaa agcaagagta cgagatattc atcgctcact
    14881 atcaattaac agataaagat ttccgtgtac agactaatat ttatttcgat accgttgatg
    14941 aacagctgaa gaaacaaaat tgtggcctac ggatccgttt cgttggtcat caagctgaat
    15001 atacattaaa aacacctgct gaaaaagggc gtctcgaaac aacggatacc tttataacag
    15061 aagaagcaga agcatttatc ttcgaaaaac gtctgcctcg ttcaggcgca gtttttcaaa
    15121 aactttctga tttgaatatc gatcctgctt cattaatcca aaccggaaag cttacaacaa
    15181 aacgggctga attccctatt gaagaaggac ttttagctat tgatgaaagc tggggagaca
    15241 atctacacga ttttgaactg gaactagagg taggggatgc atctgtggga aaaattgctt
    15301 ttataaattt tttaaaacgt ttttctttac cttatcgtcc tgctaagaac aagatccaac
    15361 gtatgcttga agctaaaaat tagaaacctt tcattttcac aaggcacttg agttcatttt
    15421 tttacttttt tctgttaagt tataggatgt gaaagaatct agtcactagt tgtatagaat
    15481 ctattttggg ggagattcat atgatcgaaa tctatttatt tgttaatcct ttaggggaat
    15541 actgtttcga aatggaacaa cagttattac aatttatcga agaagagtac gggacaccat
    15601 caaaagaaaa gatgcaattt cgctttcttc ctttggtcaa tttgcagaca attggggatg
    15661 tcatgcaaca aaaaggaatt tctcagaaca atcttgagga acgtaatcac ttattctcaa
    15721 tgacttattc tgcagcattg gactgcaaag cggcgcagtt tcaaggaaag aaaaaaggcc
    15781 gccgtttttt gatcaaattg caagaagcag tcggctgtaa tgacatacca tattctcaag
    15841 aacttgctga atccatattt gaagaaatcg gcggcgattt gaatatgttt aaagaagatc
    15901 gtcagtctga cttcgttaaa gaagtattct tttctgatca aaaaattgct cgggaaatgg
    15961 gcatcacaaa acacccttct gcagtcgttt acaattatgc ctgcgaccgt gattttggtg
    16021 ttttagtaga agtaaaaggt acaagtagcc tttggagaat cacagagctg tgtaaaacaa
    16081 cagataactc atatcaagtt tttaatggtg aacatttaca tacaaatgat tctcagttcc
    16141 attcaagaac tagccattta cacttgcttt ctaattaaaa aaatacgtcg actcccttac
    16201 aagagagccg acgtattttt ttcgtttcta ttcgagaaaa tcaaaaatag aaagccttat
    16261 aaacgatcga tcaattcttc taattcattt agacgttgtt caaacatcga cattgcatct
    16321 tcaatatatg cagcctgtgt catatcaact cctgcttttt tcattacttc cacaggataa
    16381 tcgctgttac ctgcttttag gtaagccaag tagttctcta gtgcctctgg ttcctggttt
    16441 agtattttct tcgccagtgc tgaagcagca gaaaagccag tcgagtattg gaaaacatag
    16501 taattataat aaaaatgcgg tatccgtgac cattcgaatt taatttctgg gtcttcttcg
    16561 actgctggac cataatattt tgcattcaat ttaccataac tatcacttag gtattcacta
    16621 gtcaatggca cacctttttc atcttccgta tgcatgaaat gctcgaattc agcgaattgt
    16681 gtctggcgga aaactgttcc tttaaaccca tccaagtaat ggttgagtac ataagcccgt
    16741 acacgagggt ctttttctgt ttccaataaa tactccgtta ggatattttc attcgttgtc
    16801 gaagcaattt ctgctaaaaa gatggagtag tcgccgtaca cataaggctg gttcgaacga
    16861 gtgaaataac tatgaacact atgtcccatt tcgtggacaa gcgtaaatag ctgatccagc
    16921 gtatcatgcc aattcaataa aatatatgga tttgtgtcat agcttcccga agaataagca
    16981 ccgcttcgtt tccctttatt ttcgacaaca tcgatccaac gttcagagaa tgctttttct
    17041 acgatggcca tgtattcttc acccattggt ttcagtgctt ctaaagcttt ttcttttgct
    17101 tcttcgtacg taaaggtaat tggagcttcc cctaagaccg gtgtataaag atcatacatg
    17161 tgcagttttt ccacttctaa taaacgtttc cgtaattcca tgtatcgatg taacaaaggc
    17221 aaatgcttgt ttaccacgtc taccaaagta tcgtatacac tttcaggaat atgattattg
    17281 ctcaaagacg cttctctggc agagctgtaa ttacggactt tcgctttaaa attatgtcct
    17341 tttatatgtg tgcctaaagt agaagcaaat gtatttctaa attgttcgta aacactgtac
    17401 aatcccttaa atgctgcttc gcgcaccctt cgatccgtgc tttctagcaa ctgaccatac
    17461 acgccatgag ataattggac tatttcacca ttttctcctt caatcgttgg aaaaactaga
    17521 tctgcattat tcaaaacagc aaatgtatca cttgaagcat caaagatttc acctgctcca
    17581 gcaagaagag attcctgctc agcagataag acatgggctc gattatctac tatttgctgg
    17641 atataatggc gatagacttc caattttggt tcttctttga aatattgcca aatctggtca
    17701 tctgacaatt gcaatatttc tggttcaaac caggaaacgg cttcactaac tttcgaaaac
    17761 agactgcttg ctcttgcata aagcgcttgg taatctgtat ttccagtgtc ttgatcgttt
    17821 ttaaggtgcg catatacata aatgacttca gtttggcgat aaaccctcaa tacgaattca
    17881 atcgcattta aaaattgaga agcgccttga tcaagtgttc ctttgtgttt ttcagattgt
    17941 tttaactctt ctgataattc caaatatttc tcatcaaact cttggtcgct tgaaaagatc
    18001 ttggtcaagt cccaagttaa attttcaggc aattcttctc gttttggcaa ctgctttact
    18061 tccatgaaat ccactccttt tcttccctta gctctatcta tcaaaataga tcatagtcta
    18121 acatccacat gtcacagcat ctaacttttc tactcattca tgtgtctgct tccatcaatt
    18181 ttagcataac taaatcttgt tttacataga aaaccgattc ctagcctatt tttatactta
    18241 ctattttttc aaagaagaca agtgtatgca ttcgttaaaa aactgctgat agaccatctc
    18301 ttcatcaatc ataggaaaaa gccattccct tttatgttct tttatttggc aaagccatga
    18361 gttgaaatca ggaactttct ggttttccaa ggcttcttcg tacaacattc gataaagaaa
    18421 aacctgttct tgtaaataaa agaaaaaccg acttttttga tagatccacg atgatagatg
    18481 caaaagatgt ttatgacgca agtagcattg ttcttgtatg gatactgtat tcttttgctt
    18541 gtgaataagt tggcgtgtca gccatttctt cccatcttca ctcacttttt ctattttttt
    18601 catttggttc attttaattt cgttactagt gaatatttct ttaaggtccc tactagaaaa
    18661 agtccagcag ctactgcaaa aggaaatatg ccctgataca gtctctgtaa tatgatgata
    18721 caaataaagc ttttgtttct tgagatcagc tttccagcaa tgcactcctc tttttctatt
    18781 atagtagcag caactttttt ctatcaatga gaattgcgat tgtccaagaa aagaattacc
    18841 aagtatccac catacttggt aactgttatt caaataattt tgggttcttt caataaatct
    18901 ctggtgagac agtctagaac attggatttc tattgctaga ttcgccaaca aaatatccgg
    18961 cctttgccgc aacctcggca aataggcttc caaaaatact ggttctactg actgtcccag
    19021 ccaatccatg aagcattcct ttaactgtaa gtgctctttt gattctcttt cactaaagac
    19081 cgaacagtta tctgattttt gatgggcaaa atgagcacat tttttctttc cctttttcaa
    19141 aatcactctt tttcgacagc cacgacaata atatttcttt cctttttctg catgtttagc
    19201 aaaacagcac ttattatatt cattcattgc aagcagcatt tttctccctc tgaacttatt
    19261 tacgtctaag aatttattta cacgaaaaaa gagggcgtga aaaagctatt ctgcatcaag
    19321 taataagaac agctaaacaa aaatagttcc caatattttt cgtttggttg ggcttgttac
    19381 cgcagatgca agcttttgaa cacatttata cgaaaaaaag actgcgacac aatttttgtc
    19441 acagtctctt actataatgc tgatttatac aaaataaaat ttcgttcgct cgatagcatt
    19501 acgcgccatc agacattgtc catattcatt caatgtttct ggtgtacgat cgctcttttc
    19561 agcaaactcc aaaattcgag caatctgatt ctctacatcc gcttggttga gattttccat
    19621 ccagaagtga acgactaggt aatagcgctc ttcgaataaa tacaaatcag tccaagcaga
    19681 ctctagttcg acatctgttg caagttggat cacttgttca aaatcttcaa aagcaaaagt
    19741 tgcttgtctt tcctgttctt ctagggcgct tccagataat tcttcctccg gatcgctgcc
    19801 aataatctgc tgacgaagaa agtcactcac atcttctgaa tcaacagtag aatcttctga
    19861 ttgagaaatt tcttcatccg gaagattttt actgataaac agttctaatc cgtctccttt
    19921 tggcaaaact tggaaagtga cagcttcgct tcctcggaac tcctcttcga tatcaacttc
    19981 ttccaaaata ctataaaaga aattttcgat ttctttgtga ttgccaagta aatccaaaaa
    20041 tgaaacgccc cgttcctcta aatcttcatg accaattagt acacggatcg tattttcatt
    20101 aatatgttcc atttccattt tgctacacct cactttgcca caatgtttaa tatagattca
    20161 ttgtagcgaa atcagcttaa atgtaaagaa aaaaaatctc gctgttaagc gaatttaata
    20221 aaaccagcgg atttttcaac ctttcaagct atcttattta actataacat ttttggcaga
    20281 cgaatactat atagccgact tttcagctaa ttttccctct ctatttttcg tgcaatcatc
    20341 atcttctact acctttttat acatctaatc aagaatactt tttgtaaaaa caaaaaaact
    20401 tggaaaaact atctttgtaa tagtcttt
//
LOCUS       DAJSBL010000049        20133 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.049, whole genome shotgun sequence.
ACCESSION   DAJSBL010000049 DAJSBL010000000
VERSION     DAJSBL010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20133
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.049"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(103..600)
                     /locus_tag="ONT85_002068"
     CDS             complement(103..600)
                     /locus_tag="ONT85_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HCR2379030.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
     gene            complement(730..1440)
                     /gene="budA"
                     /locus_tag="ONT85_002069"
     CDS             complement(730..1440)
                     /gene="budA"
                     /locus_tag="ONT85_002069"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HCR2379031.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNIQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            complement(1453..3117)
                     /gene="alsS"
                     /locus_tag="ONT85_002070"
     CDS             complement(1453..3117)
                     /gene="alsS"
                     /locus_tag="ONT85_002070"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HCR2379032.1"
                     /translation="MAEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVEKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAKLTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            complement(3325..3987)
                     /locus_tag="ONT85_002071"
     CDS             complement(3325..3987)
                     /locus_tag="ONT85_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HCR2379033.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGCIVMIGMTEELAKENSFSPVRLFIKMFRQFWLKGFFYWLF
                     AWIVSVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSTNCWYFQVRNPASKPNQVL
                     RIAFYYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTM
                     DL"
     gene            complement(3997..4812)
                     /locus_tag="ONT85_002072"
     CDS             complement(3997..4812)
                     /locus_tag="ONT85_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCR2379034.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTDTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            complement(5073..5516)
                     /locus_tag="ONT85_002073"
     CDS             complement(5073..5516)
                     /locus_tag="ONT85_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HCR2379035.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     HSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            complement(5650..5988)
                     /locus_tag="ONT85_002074"
                     /pseudo
     CDS             complement(5650..5988)
                     /locus_tag="ONT85_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(5976..6353)
                     /locus_tag="ONT85_002075"
     CDS             complement(5976..6353)
                     /locus_tag="ONT85_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HCR2379036.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKKIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            6577..7770
                     /locus_tag="ONT85_002076"
     CDS             6577..7770
                     /locus_tag="ONT85_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR2379037.1"
                     /translation="MTTYQRKVLVSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFVMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            complement(7936..8358)
                     /locus_tag="ONT85_002077"
     CDS             complement(7936..8358)
                     /locus_tag="ONT85_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR2379038.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQQYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
     gene            8941..9435
                     /locus_tag="ONT85_002078"
     CDS             8941..9435
                     /locus_tag="ONT85_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2379039.1"
                     /translation="MTEAESALSQAEANPTKENYATASAAIQALPENKQELTSRLAAI
                     DSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQEAIAQQQAEQARQQAA
                     AEQASQQAQAAAPEQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLSEAQARGLTPCSK
                     CYGN"
     gene            complement(9597..10769)
                     /locus_tag="ONT85_002079"
     CDS             complement(9597..10769)
                     /locus_tag="ONT85_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HCR2379040.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEGSSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(10975..11790)
                     /locus_tag="ONT85_002080"
     CDS             complement(10975..11790)
                     /locus_tag="ONT85_002080"
                     /inference="COORDINATES: protein motif:HMM:NF033902.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HCR2379041.1"
                     /translation="MTGFDENTGVALYDPAESALTFTTDTEGSISVNGLIDGNYTLVE
                     TKAPDGYQLTNPQKETTFIVKNGKLQQTTGIIHGADEANLGNPNGVVNRENTTTDNTI
                     TVENVQNYGDFHFIKQDVNTQEKLADAEFSILRAKDPTVKLMKKIANLASGEYPYAWS
                     DEVTGSDWEEVKLISDSEGKFSIEGLKQGAYCLQETKAPEGYALPKNEAAYTEFTVDD
                     DPTTSDETTIDNQPKGILPSTGGMGIIVFILVGTALVGGAVIYFKKRHQETEA"
     gene            complement(11940..12386)
                     /locus_tag="ONT85_002081"
     CDS             complement(11940..12386)
                     /locus_tag="ONT85_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HCR2379042.1"
                     /translation="MKNIKTKIWTFLGTAIMLLPFVLGLGTAEVSAAVSPTPENVTVN
                     LHKLKFTSAPENQINNGTELTFPNSEPLNGVEFNVYDITATYYPSKDTAVPADATPFA
                     SVTTSGEGLANLTLPGKSDGKDAVYVFVETPKPGVETSPNIVLSLP"
     gene            complement(12383..13387)
                     /locus_tag="ONT85_002082"
     CDS             complement(12383..13387)
                     /locus_tag="ONT85_002082"
                     /inference="COORDINATES: protein
                     motif:HMM:NF037639.1,HMM:TIGR04226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HCR2379043.1"
                     /translation="MTDSSNHKLTTIHLYPKSEQKIPAALTLTKTVENKQTDFADGDK
                     VPYLITVTIPENINEIGTYTIKDTADPQLWLEPETIDFLIGGDKIHTFHTQKIKHGFV
                     LSDSGKDLSSFAGKKLTIRYQMTLKENSEKTTFDNDVRLTTDNQTVETHLAIQTGGKQ
                     FVKVDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFTSSKDGTFSVHG
                     LSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEPKANPTNNGKTP
                     VQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKKQQKGNNRREIK
                     "
     gene            complement(13427..13756)
                     /locus_tag="ONT85_002083"
     CDS             complement(13427..13756)
                     /locus_tag="ONT85_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HCR2379044.1"
                     /translation="MFESDKMPSISQNDGINIPDNFQGVKGLNGVTFAIYDVSDEFYK
                     LRSEGSSVEDAQRKLAQKSDSEKILAENVTKTVDEEEGIASFSASDKDEQGRDAVYRF
                     TEIKTSD"
     gene            complement(13882..16209)
                     /locus_tag="ONT85_002084"
     CDS             complement(13882..16209)
                     /locus_tag="ONT85_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HCR2379045.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSAIETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
     gene            complement(17052..17345)
                     /locus_tag="ONT85_002085"
     CDS             complement(17052..17345)
                     /locus_tag="ONT85_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HCR2379046.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
     gene            complement(17748..18926)
                     /locus_tag="ONT85_002086"
     CDS             complement(17748..18926)
                     /locus_tag="ONT85_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR2379047.1"
                     /translation="MEKTDRFKKQISSTGRITWYFQAYRTTRRGFNSKREAQLAYLEM
                     EKQQRLKKKIVASNDKFSVVGEQWFQYYRSLNEQKEITYDKREEQLKIIVRWIGDKKL
                     SDLTPECLQELLFTLKECGVNGTNVGYAKNSLQSLIQVLNMIFKYCQRKNLISENPMK
                     SVRMPKYQLTVTDLKEAINNIEDKFLTVDELRTFLNYGIVHEELPMSVLFHVLFYTGC
                     RVSEALALQPGDIDFKRNEILFYKQTAVKGKSKDFRIETTKTVSSARRVLVTPLVMEK
                     LQQLIKALGEMRQHYRFIIEETYLFVYLDSSKRGVPYRREYVNDHVKRCVNRSGINKP
                     FHTHLARHTMASLVAEHCSWEVLKDRLGHTDQTTSKIYRHLTSNEKVKPLIAFSSLES
                     "
     gene            complement(19030..19344)
                     /locus_tag="ONT85_002087"
     CDS             complement(19030..19344)
                     /locus_tag="ONT85_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR2379048.1"
                     /translation="MELEVKTNVNSLEIEFYSILQKKLEDYTEKLLAVSHLPLVLTNT
                     DLKRLFQISDSTLNRLVKLGEFPPCWYGIRGHYFRDDVLEWMRKSDSNDYREKLRLLR
                     SL"
     gene            19451..19669
                     /locus_tag="ONT85_002088"
     CDS             19451..19669
                     /locus_tag="ONT85_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2379049.1"
                     /translation="MKQGIADIKIIKEILEKSTANAIAFGTGINLSTVKKLKSGERAE
                     EKLNLADAIKITEFGMKNMPTKIEIWKS"
     gene            complement(19664..20059)
                     /locus_tag="ONT85_002089"
                     /pseudo
     CDS             complement(19664..20059)
                     /locus_tag="ONT85_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317574.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
BASE COUNT         5884 a         3976 c         3450 g         6823 t
ORIGIN      
        1 gattttataa aaaaataaaa aaaccgaaaa acagaaatga ccactgtctt tccggctctt
       61 tttattcttg atttctcttc ttttttgttt caatagacga ttttagttat taataaatga
      121 aaaagcattt atattaaaat ttgctaataa aagatctaca tatttttcta gaaatgcttg
      181 atcgatttca tcataccctt tcgttactcc gcaatcgata tctaaaacac cgattaaatt
      241 accttctttt accattggca ctacgatttc tgattgtgcg gctgaatcgc aggaaatgta
      301 atttgcatgg gttttgacat catcaacgat caatgtttct tgtttttcag ctgattctcc
      361 acaaactcct tttcccatct tgatacgtgt gcaagaaact cgcccttgga acggtcctaa
      421 aatcaattca ttaccatcga ataaataata tcctgcaaag acagtctcag gcaaagtctc
      481 attcaacaat gcagatgaat ttgctaaatt tgctaaccag ttatcttctg cttctaataa
      541 tccttgttgc tgtaataata aaatttcgta tgcatctttt ttctgttttt ctgtccacat
      601 gataaaaacc gctccttttt gatctatgga gaagattata gctcagtttt tttgtaaacg
      661 ctaggtttcc tcctcaaaaa aacgagatac gtcaggttaa tcaccttgaa cgcatctcgc
      721 tgtctttttc tattctgctt gttggatttc tgctgctaaa tcagcataat caatcgtttc
      781 gtgaacaaaa gtcggatcat caactggaaa atgctgtaca agggacgaga tgcggctaat
      841 ctcaactttt ccttcagcca cttcaaaatc cattacatgt ccgccaaatg tatgtgtatc
      901 atcgataaaa tgcaggtgaa atcccgctgc agcaactcct tggaataatt caggcgtaaa
      961 gaacccgact aatgttcctt ggatattgct ttgttggaat tctggttgag actctgaaat
     1021 ttttgctagt cttctgtaag gtttttcttg cttcggcatg atacggatgt gcatattttt
     1081 gaataatcca gttatcttca ctgccaaaaa taggttttcc ccggcttctc ttactaatat
     1141 gtcttttttc aattgttctc catctgcagt ttttgagaca gaaaaactag tatctggcgt
     1201 aaaatctgta attgctgcat agggggtaag ttcacttcca tctaattggg caactgagcc
     1261 atctgatctt ccttgataag ctacgccatc taaaaagatg acttcaccat caagaccatg
     1321 caatgttccc aatcccagag ttcctttttc taatatatga gcaatcgttt ctgttccatc
     1381 catcaagccg gccatcaaag ctcctaatgt cccgtgctgg tataagattt tttcagtcat
     1441 gtcgacactt ccttaataga attgatctgg taagattgtt tctcccaatt tctcattatc
     1501 acggtaatca atcggaatat caataattac gggtccttct gtcgcaaatc cttcttctaa
     1561 agctttttct aattcctcta cacttgtagc tcggattcct ttagcgccaa aagcttctgc
     1621 atatttcaca aaatcaactg gaccaaagtc tacacctgac gcccgctgat atttcatttt
     1681 ttcttggaac tctaccatat tgtagtgtcc atcattccaa atcaaatgaa cgatattcaa
     1741 ttttttacgt accgccgttt ctaaatcctg ggcagagaat aaaaatcccc catcgccgga
     1801 aactgatacg atttgtgtat ttggacgcac caatgcagca gaaatcgccc atggcaatgc
     1861 aacaccaagc gtttgcatac cattactgaa taacaaatga cgaggttcat aacttctaaa
     1921 gtggcgtgcc atccaaatat aatgactacc tacatctacg gtaacagtca tatcatctgt
     1981 tacttttgac tgaagcgtgt taataacttc taaaggatgc aagatacctg cttctccttt
     2041 tgattgaaca atgtcccgct ccgttaattt tgcttgaaga gatgctaaat attcttttgc
     2101 atcttctgac acttgttgag gctctaatga acctgtcaat aaatcaagag tggctgagat
     2161 atcaccgatc aattctcttt caggctgcat aaaaggatcg atttcagctg gcgcttcatc
     2221 gatcacaatg atccgcgcat ctttttccgc attccagttt cttgcttcat attcgattgg
     2281 atcatagcca attgcaataa ctagatcact tcgtttcaaa agcatatctc ctggttggtt
     2341 tctaaataaa cccacacgac caaagaaatg cgcttctaat tcacgtgaga tcactccagc
     2401 tgcttggaat gtctctacaa caggcaatgc tgtcttttca actaattgcc tgatggctaa
     2461 cgtttctttt tcacttgatc ctcgcatacc tactaatagt accggtagtt ttgcttctct
     2521 aatgcgttca gctaggtaac ggatatcatc tgcagaagca gaacctaatt taggatcgct
     2581 caatggcttg atgacattac cttggacagg tgcatccact acatcttgcg ggatactgat
     2641 aaagctcgcg cctttttttg aactcttagc catacgataa gcattggcaa tcacttctga
     2701 aattgtttct ggatcttgga tttctgcact gtattttgtg attggctgga acagtgctgc
     2761 attatccatg ctttgatggg tcaacttcaa tagatcgctt cgttttactt gtccggcaat
     2821 tgctaaaact gggtcacctt ctgctgtcgc tgttaccaaa cctgtagcta agttactagc
     2881 accaggacca cttgttgcga taactactcc tggctctcct gtaatacgtc cgattgcttg
     2941 tgccataaat gcagcatttt gttcgtgacg agtcacgatt aattcaggac cttgatcctc
     3001 taattcatta aatacaccat cgatttttgc acctggtata ccgaatacat aatcaacatg
     3061 atgattaatc aaactttcta ctacagattt cgaaccttgt ttcatttttt cagccattga
     3121 acatcgtcct ttttatgctt tatttttaat tattagttag aaataatcac aaataagtga
     3181 tacagtttct aaacctgttt catacaaaag tatagcacgt tttatttcat ttgtttagcc
     3241 atatgtttca aaaaatgaaa aagaatgaca gtttttacat aaaaaaaact gtcattctta
     3301 attgaaagtt tcaatttata aagtctacag atccatagtt ctcatcaatt tacgattgtt
     3361 gaagtaaatc aaaccaaata cgatagaagg acctagtaga atacaccatt ggggctttac
     3421 gaaaaagaag agaaacaaac ttgtcaatag taaaaaatct agtaaactca cataccattt
     3481 tttcaacgta tagtagaacg ctatccgtag aacttgattt ggtttcgacg ctggatttct
     3541 cacctgaaaa taccagcagt tggtgctaaa ggaaactgat aaacaagcaa gtaaaacaaa
     3601 caacggaatc aaccattttc cataagaaaa acgtataaaa aagaaacaat caaaaatcat
     3661 tatcacacta actatccaag caaatagcca ataaaaaaat ccctttagcc aaaattggcg
     3721 aaacatcttt atgaacaaac gtactggtga aaaactattt tcttttgcta attcttctgt
     3781 catacctatc atcacgatgc aacctggacc aataaaaagt aaagaaaaaa taaaggaaaa
     3841 gacattcctt ccatctatcg acaaaaggaa cgcagctgct atatacaaga tattgacaca
     3901 ccaaaaacaa aggctacttg ccaatgattg atacaaccaa taaaagaatt ccatataaac
     3961 atttttgtca aaacgctgct ctttcataat cacctcctat tccttcattc ctgatgaagc
     4021 gatcccttct acaaaatatt tttgcatcgc taaaaatata aaagcaacag gaagaacaga
     4081 aacaactgag gcagccatga ttaatgcgta ctctgcatca aatagcccaa taaacatttt
     4141 caaccctaac tggattgttt tattttccgt agaagacaaa taaatgaatg gccccatata
     4201 atcgttccat gtattaacga atgtgatgat cgtcaaactg gcaatcgccg gttttgtaag
     4261 aggtaaaatg atcttgcgat agattcccca ctctgataag ccatcgatcc gcgcactctc
     4321 acataaagaa tctgggatac tactgtaata ttgcttcaac aagaacacac caaaagcatt
     4381 gaacgcttgt aatagcacca acgaccacaa cgtatctgtt aaacccactt ttctcatcat
     4441 aatgaactgc gggatcatat aagactgcca agggacagca atcgtgccga tatatgctaa
     4501 aaataaaata tcgcgccctt taaaatttag tttagaaaac ccgtaagcag caaaactaga
     4561 agtaaacaac tgaatcaacg tgatcacaac gcttaaaaaa gctgtattct taaaaaatgt
     4621 cactaaagga atacgagacc aaataaccag ataattctct aaatgccaag ttttagggat
     4681 ccattgaatc ggaatcgtga acacttcatt atttgctttg aatgaagcag aaaccatcca
     4741 taaaaaagga atgatcgtca ataaagctaa tacaatcagc agaatcatac ccaaccattg
     4801 aataaagggc atgttttttg aacgaatttt tgcttttttt ctttcatttc gaacacctcc
     4861 aaagttataa tcttcatttc tttgtaatga ttacaatcca gaacaacggt tattatagct
     4921 gattcatttc cgttcttctt atttttgtaa gcgcttattt aacaagttat gtaaatctct
     4981 ttattttttt gatttaaaaa tgaaaaaata caaaagcaag actgtcatac gtctcgcttt
     5041 tgtattgaaa tttcttcgat tagagatact tattactgtt caaaagcttc tttgaaacgg
     5101 tgaatcaatt cattgactac aggtgataaa atccgattct ttttccagac tagcatgcag
     5161 ttcgtttcaa tcattgggtc aagcggaacg aatgtcactc gctttgaatg atgacttaca
     5221 gcaccttcta tgactaaagc agcacctacc tgattctcaa ttagcgcaaa tacattgaaa
     5281 atcaaattgt acgttcctgt gatcagcaac tgctcctttt ttatgcctaa ccagtcagca
     5341 aacattcgct gtatttctgg acgctttgag atcaaaagag gtacattcgc aaagtcttct
     5401 tttgttatca cgtctttttt tgctaaaaca gaattttcta aaatgagcaa tccccatttt
     5461 tcctttcttg gtaacaaaat cgtttcgtat ttctctgtat ctactggatc tagcaaaatc
     5521 gctaaatcta acaggctttt ttctagtcac tccgtgatat catcacttgt tccgctgtgg
     5581 atatgaaatt gaacgctggg ataatcacgg atcaactttt ctagtaatag tgccaatgta
     5641 ttcgaactat cagcttccac acagccgatc gtaaatcggc cgctgaataa ttgttttttt
     5701 ctgttctcga attcttgagt ggtttgattc gttaaatcta aaatttcttc tgctcggctt
     5761 ttttagaaaa taacctgctt ctgtcaagat cattttgcgg ttttcgcgta caaacagttc
     5821 tgttcctagt tactcttcta actccttcag ttgtcggctt aaggttggtt gtgtaagatg
     5881 tagaacttct gcggcttgag agattgttcc ttcttctgca attgtccaaa aatattttag
     5941 taagcgtaac tccatagcga tgctccttga tttttttact tgatacattc cattttattt
     6001 ctagataaca cacgatcata tttcttgatt tttccttgta aacgttccaa acattcttgc
     6061 atttcagcaa tttttttcgt caaaagttca ctttcttcaa tcaaaagctt acgccgctca
     6121 aatacagttg cttctccttc gctagatagg cgaatatatt caatcaaaga ctcgatcgat
     6181 aatccggcat ttcgcataca gatcgtaaag tcgacccatt ttaggtcttc ttctgtataa
     6241 tcacggattc cgccttttgt ccgattaact ggtggaatca acccgattcg ttcataatag
     6301 cgcaatgtat cggccgaaat gcccttttct tcactgactt gtttgatatt catctgatca
     6361 ctctttctct taatttcttg ccactttact tatgtatttt atttattcta caactttaag
     6421 tgtactccaa gtcaatactc atttctatta aaactaagct ggattatcta tatcggttgt
     6481 ttgtgtatta tttcaaataa aaaagtcttg tttttttatt cttttttgga tatactttta
     6541 acaatttagg gaaattttag gaaaaggggt ataaaaatga caacgtatca gcgaaaggta
     6601 ttagtatcga catcggcggg aattgcttta gagaatatgg atattatgtt tttagctttt
     6661 tcattatctt caatgattac ttcttttcat attagtggta cgcaagcggg tttcatttca
     6721 acgatcacta atttggggat gctcattgga ggtatttttt ttggcatcat agctgataaa
     6781 tatggacgtg tgaaagtatt ttctcaaacg gtccttctgt tttcgattgc ttctttattg
     6841 atgtattttg cttcaaacat ctacttggtt tatctctttc gctttatcgc aggtgtagga
     6901 gcgggaggag aatatggggc gtgtatgtcc ttgatttccg aaagtttttc aaaaaaacaa
     6961 attggacgag cttcttcggt tgctggtatt ggtgcgcagg tcggtgcggc tttggctgcg
     7021 attttagctg ctgtgatcat tccaacgttt ggttggaaga tgttgtatgt cgtaggtgta
     7081 ctgccggtgt tgatggtctt gttcattcgt cgcggcttga ctgaaccaaa ggaattcaaa
     7141 tctgtacaat ctgccgaaaa gaaaaccaaa cttagcagac tgtttgaatc aaaacatctt
     7201 gcttggcaaa catgtggcct tagtatcatg gtaactgtcc aaattgccgg ctattttggg
     7261 ttaatgaatt ggctaccttc aattatgcaa aatcgattag ggctttcggt ttctgggtct
     7321 tcattgtgga tggtcagtac tattttaggc atgtctttgg ggatgatgac ttttggcata
     7381 atcatggata aattggggcc taaactagcc ttttctttgt ttttaatttg ttcatcctta
     7441 tctgtctatc tacttgtgtt agcccatagc cagtggagtt tggtagcagc cgctgtgata
     7501 gtcggctact ttatcaatgg catgtatggt ggatatggcg cagtgattag tagtttgtat
     7561 ccaacagaaa tccgcgcgac ggcgaataat ttcgttatga accttggacg agctgttggt
     7621 ggtttttctt ctatcgtcat cggtttcttg atggatcatt atagtttaac tgccgtaatc
     7681 ttgtttttga gcggtattta cctagtcagt ctgctggtct tgacaactct tactggtgtt
     7741 acagcattaa gggaaagctt gcagcattga tagttgagtc tgtaaatgac aagaaagagg
     7801 tgtggaaaaa gcttgcatct gcggtaataa gaccagccaa atcaaaaaca tgagacactg
     7861 tttccgtttg gctggtcgta ttacttgatg cagggcagct ttttcaactg ctaatttcct
     7921 tctatctggc ggcgactatt ctttgacgtc cgtcatttca aagtacttta ctaataactt
     7981 agcataacga aaaactgtgg atacactcgt atattgagcg tctgcataat cttccaaatt
     8041 tttctcagga tgttgataca tgaattcaat caaatgataa ttatatgatt ttctgtagaa
     8101 atgaagataa ataatgtaga ttgggaagtt ctcctgcttt tccatagaaa agcgccgatt
     8161 attctcattc ttcaaaatga ccttatcagg atattgttgg aacaattctt ctaaatctgt
     8221 tagataattc aagatagact tacttgatac cttcaattcg ttttgtagtt cttctattga
     8281 acaccaacgt tctgtttcaa gcagtaaatg aatcatttta attttctttt gagaaacttt
     8341 tgttaaaaaa gtctgcatat tttctcctcc tgtatctact ttactatact acatacaaga
     8401 aaaaaacgga tacaaatatc atattgtcac accgtcaata gtgcataaac ctgttattat
     8461 ttctcttttt ttcctaagta ttctattgat atgaatcatt taatcgttta ttatataaat
     8521 gtcaataatc taataataaa ataacgtaca tattttatta ttgcatactt aataacagaa
     8581 ctatagcaaa aaatcccgtg tatcaacacg ggattagatt aagtttcctc aatcactatt
     8641 cataacacaa ttattataac aagtaagtaa acaattgaaa aggacattta ttgaaaacgg
     8701 ttgtttttta cttaaggagg aaatttagtg aaaaaattag ttgcgttgtt tttggtcgca
     8761 ggaagtttgt tgagtgggtg tggtgctact acgtctacta ctcaaagcac aagttcagct
     8821 caggcttcca gcagcagtac cacgatctcc gaaagtacgg cagattcatc gaaagaacaa
     8881 attgaaaaga aaaaagaaga agagaaacaa aaagcggaaa aagctgcact agaaaaaaaa
     8941 gtgacagaag cggaatctgc tttgtcacaa gctgaagcaa atcctacaaa agaaaactat
     9001 gcaactgctt cagcagctat ccaagcgcta cctgaaaaca aacaagagtt gacaagccgt
     9061 cttgctgcaa ttgattccac aatcaaagca aaagaagcag aagaagaacg gaaaaagcaa
     9121 gaagaaattg cacgacaaca agcagaacaa gcgcaacaag aagcaatcgc acagcaacaa
     9181 gcagaacagg cgaggcaaga agcaatcgca cagcaacaag cagaacaggc gcgacagcaa
     9241 gcagcagctg aacaggcttc tcagcaggca caagccgcag ctcctgagca aaataatgaa
     9301 caaactgtct atgtcacacc gaccggttca aaataccata cacataaatg tggaaatggt
     9361 acgtataccc ctgccacttt aagcgaagct caagctcgtg gtttaacccc ttgctccaaa
     9421 tgttatggaa actaattcaa aagttatact ataataataa aattcttctg aaacataaaa
     9481 aaatagaagc tggcgttcca gatagtaaac caaaaatagc agtttacacg tccatttcgt
     9541 cagcttctct cttttttcat ttagtctgtt ccctctgctt ttttggacat cttcattcat
     9601 tctttctcaa tcaaatagta cacctctttt tcttttcgca gtttatatct tccttttttc
     9661 aatttcattg aaaactgttt cgaatgttta tgttttacat ggattttaag caggagctcc
     9721 tcatgttgca agcgatagtt cctgcctttt aaatcagaaa atctcactag tccgtattca
     9781 tcagaagctt tcacaaatgg aactttttct ctagtaatat agtgtttgcc gttgagcgtt
     9841 tttacagaaa agaccattcc ttcgattggt cgtccgtttg tgttctttac ataaaaagat
     9901 aaaggatagt ttcgcttagc gatggccaaa gaatctaaat aagcgatgag tagccaaact
     9961 aagccgattg atccaagtag tataccgata aaccaaataa aaaatttcca ttttttccat
    10021 tgatcaattc cttggatcgc tttttttgcc tcttttgctt gataaggaat acgatgcccc
    10081 gtcacaagca gtcgatgtgt attgaccata taaggagtgc aggtaagcaa agtcacgaga
    10141 tctctgcctt tttggatacc caaagaatct acttcgtcag gtaagaccac ttggattttt
    10201 tctacttgat acgccaatgt ctttccattg atctggatga aaaactggtc tccttttttc
    10261 aacttaggca aatctgtaaa taattttgct tctgggagtc cacgatgccc agaaagaacg
    10321 gcatgggtgt ttttcccgcc agtcggataa gaacttcctt ccaataatga cgttcctttt
    10381 tgcaaaaata tttccgttgt ctgatcgaat ataggtaagc tgacattgat ttttgggatt
    10441 tcgatgactc caagcatatg ttccataaaa aaggcattac taggtaaatc ttttaatgct
    10501 tttgcatcaa ctgcttggtt gaaactgcct atccctggtg atacgttttg ctctgccaac
    10561 tgttggtttt tctttgtcat tttttcctgc tgttttttta tttcctctga attcttattc
    10621 gatgcttgct tttgataatg gatgatcatt tgctgggcaa gaaaatcatt cagtgaatct
    10681 tctacaaaag gataagaaaa gatcccgatc ccaacgacta aaatgattgc catgaagatg
    10741 tccagtattc ttattctgct tttattcatc atgtccacct attttctaat atgtatatga
    10801 agaggttgtg aaatcagcgc cttgacctga gtattagcga agcaaacggc aaaatagttc
    10861 ctcctatttt gccgtttgtt gagcgaatac caagagaccg accacttgtc acaacctttc
    10921 tccccatttg aatcactatc gtttatttat cgtatgattt ttgtttccaa tagtttaagc
    10981 ttctgtttct tgatggcgtt ttttgaaata gatcactgct cctccaacta aagcagtacc
    11041 taccaagatg aatacgatga tccccatacc accagttgaa ggcaagattc ctttaggttg
    11101 gttatcgatc gttgtttcat cagaagttgt tggatcatca tctactgtaa attcagtgta
    11161 cgcagcttca ttcttaggta atgcgtagcc ttctggtgct tttgtttctt gaaggcagta
    11221 tgccccttgt ttcagccctt cgattgaaaa tttcccttct gaatcagaaa tcaatttcac
    11281 ttcttcccag tcagaacctg tgacttcatc tgaccaagca tatgggtatt ctcctgaggc
    11341 aaggtttgct atttttttca ttaatttaac agttggatct tttgctctta gaatagaaaa
    11401 ttctgcatcg gctaattttt cttgcgtgtt cacatcttgt ttgatgaagt ggaaatcacc
    11461 gtagttttgg acattttcta cagtaatcgt gttatccgta gttgtatttt cccgattgac
    11521 tacaccgttt ggattcccaa gattggcttc atctgcgcca tggatgatac cggttgtttg
    11581 ttgcaatttt ccgtttttta ctataaaagt agtttctttt tgtggattgg ttaattgata
    11641 accatctggt gcttttgttt caactaaagt ataattgccg tctattaatc cattgacact
    11701 gatggatcct tcagtatcag ttgtgaatgt caacgctgac tcagcaggat catagagagc
    11761 gacaccagtg ttttcatcaa aaccggtcac gaattgattc tctgaatttt tgatctggaa
    11821 ttgcgcccct gctaacttat ctacccctga aaagttccca gctttctgga ttttgatatt
    11881 gctttctttg atgacatttt tagcatatag gtgaatcgtg tctaaaggtt tgtcagggtc
    11941 tagggtaagc ttaacacgat atttggcgac gtttcgacac cgggctttgg tgtttctaca
    12001 aagacgtata cagcatcttt cccatcagat ttacctggaa gagtcaggtt agctaatcct
    12061 tcaccagaag tcgttacact tgcgaagggt gtagcgtctg ctggaactgc tgtgtcttta
    12121 cttggatagt acgtagcagt gatgtcatat acgttgaatt caacgccgtt caacggttcg
    12181 ctattcggaa atgtcaattc cgttccgttg tttatttgat tttctggtgc tgaagtgaat
    12241 ttcaatttat gcaaattaac cgtaacattt tctggtgtag gtgatacggc tgcagaaacc
    12301 tctgcagttc ctaggccgag aacgaaagga agcagcatga tagcagttcc taaaaacgtc
    12361 catatttttg ttttgatgtt tttcatttta tttccctcct attgtttcct ttttgttgtt
    12421 ttttataaaa ccaagctgtc cctgttgaaa ggactagcaa taaccctaaa attgagaaac
    12481 ctccaagcat ctcttcgctt gtttttggaa agcttcctcc atttttttca tttgatggac
    12541 catttgtctg catctgaact ggcgtttttc cattattcgt cggatttgct tttggttcgt
    12601 tgatgatttc caatggtata ggatgaagcg gatcataact gcctttttca acttcaaaga
    12661 gaatggttga ttgactcaat atatatcctt taggcggttt aatctccact agttcgtaac
    12721 ttccaaaaga caatccatgg acagaaaatg ttccatcttt actggaagta aaaatcgttg
    12781 ctcttgaatc atctttggct acccaatgat ttatcccgtt ttcttgcgct aagtattggt
    12841 catttttttt gactagaaat tgagcatctg ctagtttttg tttcgtttgc cgatccactt
    12901 tgacaaattg tttgcctcct gtttggatgg caagatgtgt ttctactgtt tgattgtcag
    12961 tcgtcaaacg tacatcgtta tcaaatgtcg ttttttcact gttttctttc aatgtcattt
    13021 ggtaacggat cgttaatttt tttccagcaa acgaactaag atcttttcca gaatctgata
    13081 agacaaaacc atgttttatt ttttgtgtgt gaaatgtatg aatcttgtct ccaccaatca
    13141 aaaaatcgat cgtttctggt tctagccata actgaggatc agctgtatct ttgatcgtat
    13201 aggttcctat ctcgttgatg ttctcaggaa ttgttacggt gatgagataa gggactttat
    13261 ccccatctgc aaaatctgtc tgcttattct ctactgtttt tgttaatgtg agtgcagcag
    13321 gtattttttg ttcgctcttt ggatacaagt ggatggtcgt caatttatga ttagatgaat
    13381 ctgtcacagg aagcacaacg acaaatggtg ctgatttttc ttttacttaa tctgaagtct
    13441 ttatttccgt aaatcggtaa acagcgtcac gaccttgctc atctttgtcc gatgcactaa
    13501 aagaagcaat gccctcttcc tcgtctactg tttttgtcac attttctgcc agtatttttt
    13561 cactatcact tttttgtgct agttttcttt gtgcgtcttc cacactactt ccttctgacc
    13621 gcagcttgta aaattcatcc gagacatcat atatagcgaa agtaacgccg ttcaaccctt
    13681 ttactccttg aaagttatcc gggatattta ttccgtcgtt ttgactgata cttggcatct
    13741 tatcgctttc gaacattcga ttatgcaaag taaatgtaat aggtgattct ctagctgaca
    13801 cagtcggaga acctataaaa aagaccagta ggataaaaaa gagaacagca aatatatcca
    13861 cacgtacatt ttttgttttt ttcatcgctg ttcacctgct tttttcttac gatggataaa
    13921 ataaaagaag ccagaaaaaa taaagatcag tcctaaaaag acaaagggca gtagacctat
    13981 cccacctgtc gaaggaagca agagagtcgg attgatcgtc aacttagcgg tatctgttgc
    14041 gataatcgta taattattgt ttacacttcc ttcgcctaaa ctaatcgtta ctatttgtga
    14101 tccaatatct gtccggctgt taactgttag ggtataatcc aatgattcgc ctaagacaac
    14161 accggtaacg tggagatttc caaccgaaat cgagtgactt tgggcatcat aattaaaggc
    14221 agcgtcatcg acagtaatca ctgcgttagc tttatagatg gtataagttc ctgatgtgtt
    14281 ggctatatca gtaaatgaca acttgggatt tgctgctttg actttgtcta ttccgctatt
    14341 cgttagttta attgtatagc tgccagcatt aaccatattt tttgctgatt gtgcatctgc
    14401 tgtgtaatcc atactgctaa gtgccacttg tactggagta agcgaactag tgacaccctc
    14461 tgctagcttg acatggattg acgggacacc tgcttttcca tcatatgtct tactgccact
    14521 gagctctacc gcaccgctga tttgcttaat cgtatagaca tcagatgtct gcacgatagt
    14581 aaataaatag tttgttctgg cagctagttt agctagtccg ctttttgtca attgccaaga
    14641 cgtataggtt ccaatttcag taggcgcagt tacttcaact cccttttcat tcatcgcttt
    14701 gaaatcagtt ggattccatg tgatttgatc aatactgaga tcattgttta aaagggatgt
    14761 tgattctgtt ttatcattaa aggcatattt aacgatcggt acaacagaaa gaccatcata
    14821 gtacttactg ccatttgcat tgattgatac tgttgcattt gtcttataat ataggatgac
    14881 ttcagaaatc tgttttccat ttgcgtcctt tacgattaca tttccatttg tatctaaact
    14941 agctaaaaca ctcattgctg attggttctt tgtttctatc gctgaaacag ctgtaaaggt
    15001 atatccgttg atagtaggcg tattagtcgt caggtcttgt gcagcacttc cgtaagtaag
    15061 attcattatt ttagaaggag caatcatctg tccaaaatag tcttgatact taatagtgat
    15121 tggtgtttgt ttgcctgtat aggtcaccgt atcgacgtta ctttctaggg ctgtgaaaga
    15181 accgtttgtt tgtgcaaaag aatagcccgt tactgctgtt ggagtgataa ccactttgtc
    15241 acccacatgg attccagata atgtagtccc gtacgttccg atttttgctg ctccaccatt
    15301 gactgtataa gaaaaagttc ctgtccgatt cgcattgacc tgaaccgttg ctgcttgata
    15361 ggttggaaaa tttgaaacat ttcccttacc atcaacgatc tgcccatttt tgatcgagta
    15421 ccccgtgaat ggaatatcat ttgctaactg tataccgatt gaaaaagtac tcatacctcc
    15481 tccggcagga agccctcccc atgttgaggg tgtaaaagaa taacttttat ccgcattggg
    15541 tgtaactgaa tcgatcgtat ttccagcggt agccaccccc atatagatac tttttgggaa
    15601 tgtgatagta gtaccattta ctgtggagcc aataaacaga ttatggatat ctggacggtt
    15661 atcaataaaa gcgattgctt tggttacgcc ttgtaacgca gttttatccg cactcatcga
    15721 tccgttaacc gcaacaatga ctccatcttt ggcatatatc actgtatatc catctggtat
    15781 ggtatgtccg ttgaaattcg cagtggccgc aacagtacta aatgtctggc ttctacctgt
    15841 ttctgaataa gctttctccc ctgctgcttt tgctgctgtg atttcttttt ctgtcggatc
    15901 aatcagttga gtcgaggatg tggttgaggt tgtctcgttg ttgctagact gattggtgct
    15961 ttctatggac tgtatttcag aatagggtgg actatttgtc tcctctgtcg tttggttagc
    16021 aaaatttgac gtttctgtat aaacgtttac ataaatactt gataagctcc ctaagaaagt
    16081 aaaaatcaag atgatcgaaa tcataatggg atgtaagtat tttcttttag atacagcttt
    16141 gagtctagaa acaaaatgtt tttgacatat acgttggatt ttattttgag ttttcttaat
    16201 cattttcatc cctcctctca ataaaaaatc atcaaataat cctttacacg ttattctaaa
    16261 tttaattttt attattttat tgatgtatag aaagtttggg gctttttttc tctttttatt
    16321 ctaaggttat tttagtcttg tcctttaata caaagatttt atttttaaat aaaaaatact
    16381 ctttttttac gattattgaa aaaaaaagat aaaacttgat taattgaagt attgcttaaa
    16441 tttggaagct atgagggaag aataaaagag ttaggacggg attatctact atttttcttt
    16501 gttttaaata tttgttatct ttgaacataa aagagaaatg tgtcgactgc agtgttgtgt
    16561 ccctcatcac gaataaacgg tgttcaatta gctgatcttg tattcactta aaaaatggta
    16621 aagtacgagt tgctattccc cattttcaat tttctaacgc tcaggacaaa atactgtttt
    16681 cacaacctca ttggaataaa agcatatata ttgtttcaag aacgaaatct tgttatcttt
    16741 ttaaagggca tctgtccaaa atctaatttt aggaggtacg ttttggtatt tattcgtaca
    16801 aataaaagag aattatcttt attcatgtaa cgcatccaat tgattttttc atgtacgctc
    16861 ctggaacgat tttactgacg attgcttatt taactgcgaa tcgctcatta gcttttatca
    16921 tagctatcca tattttgaat aacgttcttg gctttgtatt atagatacag atgacaaaaa
    16981 aagttcaagt atgcggtgta gaaccaatat cgcatacttg aacttttttc aatgagtata
    17041 aattaaaact tctaattttc aattcccatc atctgatatg cccgaccgtc tgaacttctt
    17101 ttgaaaaagc caaatgtgat tagataacgt ctttgttcga cataatcgcc actagaaaat
    17161 acgttcagca gtatcgaatt aacctctgtt tctgtataag tcactccatg gtcaaagcgt
    17221 ttcaagactt ctttcaatag gatgtatttc ttttttgttg aacttggtac ttgttttact
    17281 tttccatcat tcatgaatgt gtctaaaact ttcatctttt cttgctccgt ataagtataa
    17341 accatcttct ttctcctttt gattctaaga atactccatt tatgttagaa tattctggat
    17401 tttttattaa agcggttact cttaagaaag atattatcat agtgctcttt tttttactac
    17461 tacttatagg tagatttatt ctaatccatt attctcccct accaataatt aaagaaaaaa
    17521 ctggatcagg gtgttcaaat tacatgctaa gaaaaaagaa cccttgaata ccaagggttc
    17581 tttctcagtc tgataacaat gattaacgac ggatttcttt gatacttgtg aaaagtcgtt
    17641 aaagtattga tataaaagga tttttattac ctctgactac atattgacta atttgttaaa
    17701 aaatcgtttt tcaagaatcc tgtaatacca gtgctttcac cgttttttta actctctaaa
    17761 gaactaaaag caatcagagg ttttaccttt tcatttgacg ttaagtggcg ataaattttc
    17821 gaagttgttt ggtcagtatg gcctaagcga tctttcaaga cttcccagct acagtgttcc
    17881 gctactaagc ttgccattgt atgacgagct aaatgagtat gaaatggctt attaattcct
    17941 gagcgattca cgcaccgttt gacgtgatca tttacatact ctcgacgata aggaacacca
    18001 cgtttactag aatcaagata cacaaacaaa tatgtttctt ctataataaa cctgtaatgt
    18061 tgcctcatct cacccaaagc cttaatcaac tgttgtagct tttccataac tagaggagtt
    18121 actagaactc gtctagcaga actaactgtc ttagtagttt caattcgaaa atccttactc
    18181 ttacctttaa ctgccgtttg cttatagaac agaatttcat ttcttttgaa atctatatct
    18241 ccaggctgta gagcaagcgc ctcgctgact cggcagccag tgtaaaatag aacatggaaa
    18301 agtacagaca tcggtaattc ctcatgaaca atgccataat tcaaaaaagt tctcaattca
    18361 tcgacagtaa gaaacttgtc ctcaatatta tttatggctt cttttaaatc agttaccgtt
    18421 aactgatatt ttggcattct cacagacttc attggatttt cacttatcaa attctttctt
    18481 tgacaatatt taaaaatcat atttagaact tgaataagtg attgtaaact atttttggca
    18541 tagccaacat ttgttccatt aacgccacat tcctttaagg taaaaagcaa ttcttgaagg
    18601 cactctggtg tcaaatcaga caacttttta tcaccaatcc atcgaacaat aatttttaat
    18661 tgttcctctc gcttatcata ggttatttcc ttttgttcat ttaacgaacg gtaatattgg
    18721 aaccattgct cacctacaac actaaacttg tcattgcttg caacaatttt tttcttaagc
    18781 ctttgttgtt tctccatttc cagataagcg agttgcgcct cacgttttga attaaagcca
    18841 cgtcttgttg ttcgatatgc ttgaaagtac catgtgatcc taccagtaga actgatttgc
    18901 ttcttgaatc gatctgtttt ttccatttat tgattcctcc tattttgaag gaacccaaca
    18961 agcagaaacc ctttggaaat ctttctatct atgtcatcat tgtaccgcat cttagaaaga
    19021 aaatgtaact tataaagatc gaagtagtcg caatttttct cgatagtcat tcgaatcact
    19081 ctttctcatc cattctaaaa catcatcacg aaaatagtgt cctcgaatcc cgtaccaaca
    19141 gggaggaaac tcaccaagtt ttactaaacg gttcagtgta ctatcactta tctgaaatag
    19201 tcgctttaaa tcagtatttg ttaataccag aggtaaatga ctgactgcaa gcaatttttc
    19261 cgtgtaatct tccagttttt tttgtaaaat tgaatagaac tctatttcca gagaattaac
    19321 atttgtttta acttccaact ccatcaccct tctctctcaa aatcaaaacg accaccacgg
    19381 taccccttaa aattgtaaaa aaacactcca gtactccatc aaaacggact agaaaagagg
    19441 aatttttaat atgaaacaag gaattgcaga cataaaaata attaaagaaa ttcttgaaaa
    19501 atctacggct aatgcaatag cattcggaac aggaatcaac ttgagtacag taaaaaaact
    19561 taaaagtgga gagagagctg aagaaaaatt aaatcttgcc gatgctatta aaatcacaga
    19621 atttggcatg aaaaatatgc caaccaaaat cgaaatatgg aaatcctagc tcttccaaag
    19681 ccatttgtcg ttggtaccaa acactgccga gacttttccc aataatatcg ccaatttcac
    19741 gatcattaag accgatgaag aaggacataa aaatgatttc ttgtctttga ctaggtaatg
    19801 ctgcaatcgc atttgccaac tcctcattgg aaataaagat tttcatacca aggacaataa
    19861 aagtaccact actattcaaa tttggttcca aataatagaa ctctgtacct aacccttttt
    19921 gttccaatat ttccattgat atttgccgtt ggcgaaatct agcgtacttc ttttgaatat
    19981 ttaacgcctc attgcagact actttcttac aaaaactgtc aaacgtatgt tgaatgtggg
    20041 tttcctctga gtcgcccata atattctccc cctcaaaaaa ttcattcaac tcgttttact
    20101 tctatccctt tccattatta ctacaatcta gga
//
LOCUS       DAJSBL010000050        19821 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.050, whole genome shotgun sequence.
ACCESSION   DAJSBL010000050 DAJSBL010000000
VERSION     DAJSBL010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19821
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.050"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            6..305
                     /locus_tag="ONT85_002090"
     CDS             6..305
                     /locus_tag="ONT85_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCR2379050.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="ONT85_002091"
     CDS             378..1739
                     /locus_tag="ONT85_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HCR2379051.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="ONT85_002092"
     CDS             1761..2021
                     /locus_tag="ONT85_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2379052.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="ONT85_002093"
     CDS             2035..2787
                     /locus_tag="ONT85_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HCR2379053.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="ONT85_002094"
     CDS             complement(2881..3486)
                     /locus_tag="ONT85_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR2379054.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="ONT85_002095"
     CDS             complement(3539..4513)
                     /locus_tag="ONT85_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HCR2379055.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5610)
                     /locus_tag="ONT85_002096"
     CDS             complement(4891..5610)
                     /locus_tag="ONT85_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR2379056.1"
                     /translation="MPKYEEIANILRERIRSKEYPLDSFLPNQVDLVKEFGASRMTIK
                     KAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEYEGLSLQMKRRHRSLKSQIIH
                     FDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVLEHTFMPVHLVPGLKKEHLLA
                     SIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAENDPVLEVQQVVYLKDGQPIE
                     YSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="ONT85_002097"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="ONT85_002097"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HCR2379057.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="ONT85_002098"
     CDS             7161..8558
                     /locus_tag="ONT85_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HCR2379058.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="ONT85_002099"
     CDS             8639..9079
                     /locus_tag="ONT85_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HCR2379059.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="ONT85_002100"
     CDS             9227..9553
                     /locus_tag="ONT85_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HCR2379060.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..10303
                     /locus_tag="ONT85_002101"
     CDS             9554..10303
                     /locus_tag="ONT85_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2379061.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10383..10838)
                     /locus_tag="ONT85_002102"
     CDS             complement(10383..10838)
                     /locus_tag="ONT85_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HCR2379062.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
     gene            complement(11265..11540)
                     /locus_tag="ONT85_002103"
     CDS             complement(11265..11540)
                     /locus_tag="ONT85_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2379063.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
     gene            11834..12649
                     /gene="thiD"
                     /locus_tag="ONT85_002104"
     CDS             11834..12649
                     /gene="thiD"
                     /locus_tag="ONT85_002104"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HCR2379064.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
     gene            complement(12978..14429)
                     /gene="cls"
                     /locus_tag="ONT85_002105"
     CDS             complement(12978..14429)
                     /gene="cls"
                     /locus_tag="ONT85_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HCR2379065.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            14770..17436
                     /locus_tag="ONT85_002106"
     CDS             14770..17436
                     /locus_tag="ONT85_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HCR2379066.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            17800..18552
                     /locus_tag="ONT85_002107"
     CDS             17800..18552
                     /locus_tag="ONT85_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCR2379067.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(18574..19728)
                     /locus_tag="ONT85_002108"
     CDS             complement(18574..19728)
                     /locus_tag="ONT85_002108"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HCR2379068.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYGLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
BASE COUNT         6330 a         3473 c         4060 g         5958 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aacccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaatttta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag attattttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacctgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc cttaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg atgggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagaaga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cgtcaattca ctcaaagcgc ttattactgg tgaatgtatt cagatttgta
    10321 aaaatattct atttatccgg cgtagaaaaa aagactgtga cacgcttttg tcacagtctt
    10381 ttttatatat aataatttaa aaattcatca tctaaatccg tagaattttc tagattgctt
    10441 acccatgtaa aatcgttata gacagatttc agttgacttc tgcgccattg tacagggata
    10501 aaaagacgca tgctatcttc attgaatccg actaataatt tctttccgtc agtaatgatg
    10561 ggacgttgta acaaagtaaa atgcgagacg atcagatcga caagctctga ggtcgtacat
    10621 tggtcgattt ggtctttcaa atttttataa tcaatcgagc gtttagctaa tagatcgtca
    10681 aatccgtttt ctgttaatga caataaataa aatatctctt tccttgttaa aggctgttta
    10741 ggtgtgaaaa ttcgttctct gtaaggcatt tgatggtctt tcagccagtt tgccatcaga
    10801 tggtaactat gtgtgcgaga agaatagatc gtaagcataa aaaaactcct taaatagtcc
    10861 attaatttgt tagatctgtt gagataaata ataggtaagg agctgtgcat aagcattgat
    10921 caaaacaacc caaacatcat tagaaataac tttctaaagt cttaaacttg cgacgagaaa
    10981 caactagact aatagaaaaa atgtcttagc catttggctt cctgtttgtt tttttgctta
    11041 tgctgcagct cctcgggaaa gggttctaac aatttgaaga aggttgatga ttgatctagt
    11101 ttaaattgtg tgaaatttga aatctggtgt ttttactaga tgaatcacct aacaactatg
    11161 attataactt atagaacttc tgttttgcat tcggttttgt tatagttgtt ataaagtttt
    11221 attttattac agaacatttt taattaataa agaacagtta gaagttaaaa ttgctgattc
    11281 acatagagtt tatgacttct tggaaattct ttttcaatca gttttttcat ctgtttatga
    11341 aattcttttt tttcagtaaa cataaaggtt tgggtatcgt aaccttcttc atgtaggaaa
    11401 tagattattc ctttttctgt gttttgcgta taagctttta tactcctgtc ttttttgaaa
    11461 gaacagatcg tatagccatc tatttggtct ggtagtcgtt tgattttctt caaaatgact
    11521 tttttatcct caagtttcat ttttttcacc tgactttatt tttttcatat ggaaatcata
    11581 gcgcctttgg ttatatgaaa acaagaagga tactgaaata tatataaatc aaatagtggt
    11641 tatttattat tagaatagcg aatacaaata aacgaccact attttctgat ttattgcaaa
    11701 tgttttttca aataaatttg attaccaaga aaaggtagtg gtatatttac tgagggatac
    11761 tttgaaaaac atatgataga aacagtaaca gtattatgaa aaatcttttt ataaaaatgg
    11821 aggaatcgat ttgatggaag atcagtatgt acaagcaatg acgatcgcag gatcagatag
    11881 cgatggaagc gcagggatgc aagcagatat gcatacattc tttacaagaa atgtttacgg
    11941 tgtttctgtc atgaccgcat gtgttgcagg taattcttac ggaatcggag caagtgtgac
    12001 attgccaaca gattttatcg acaaagaatt tgaactgatt gcgaaggact ttcaaatacg
    12061 tgcagctaaa acaggtatgc tagcagattc aaagttgatt gagacagttg taaaaaatta
    12121 ccaaaaatat gattttggac cattagtcgt ggatcccgta atcgtaacga aacatggaaa
    12181 tcttttacta gaagaatctg ctttgcaaag cttgaaagaa aagcttgtcc ctttggccga
    12241 agtactgaca cctaattttt atgaagcaga aaagctgact ggacggacat tagcagctga
    12301 agaagatttt gtgcaagcag caaaagaact gcaagatatg ggtgcaaaaa acgttatgat
    12361 caaaggacat catgctaaaa atgcgcagca agaaacagtc aatgactacg tattgttcga
    12421 atcaggaaaa tcattttggt taaatgcacc attttatcct accgaccgga tcaacggaac
    12481 tggcgattcg ctatctgcat gtattgcagc tgagttagcg aaaggaaaaa cagttgaaca
    12541 agcgatccga ttggcaaaag actatgtaaa ccgtgcaatt cgtgaagaaa taaatgtagg
    12601 gcacaaatat ggccccatca atcattgggc agctggtgaa ttagagtaaa agaaagaagt
    12661 tgtgaaaaag ttgccctgca tcaagtaata agaacagcca agtaaaaaca gcttcttatg
    12721 tttttaactt ggttggactt attaccgcag atgcaagctt ttgaacaccg tttaagaaag
    12781 aggtcgtgag cctgccgtcc agctccgagt cacaaggaac aagttcaaaa aacagcttct
    12841 catgtttgtt gaatattgtg acttgtgacc gaggagttgg caggagaaca ccgtttgttc
    12901 ggaagaaggg actgtgacaa gatttttgtc acagtccctt taatcttagt atatttttct
    12961 ctacttgaat aaaaaaatta taggattgga gaaaataatc gagaaatttg ttgcttgaat
    13021 aatagccagt tagacatttc cttgactgtt tcagttgtca tggatgaaca atgtgtctca
    13081 tcttctttga actgagtagc aagttcttct aaaaactcag gatcgtaaat gacagcactt
    13141 gtttcaaaat tcaatcggta gctacgaata tcttgatttg ctgaacctac catgcagatt
    13201 tcatcatcca ttatcaatgt ttttgcatgg aggaagccgt tctcatagac aaggatttgt
    13261 acattttcct tgatcagctg ccgagcataa tattgtgttg ctcgataaat aaaaggatga
    13321 tccggtttgt tgggaatcat gattttcaca tctacaccag aggcagttgc gatttttaaa
    13381 gcagcaatga cactttcatc aggaacaagg taaggtgtct gtatccaaac acgtttttta
    13441 gcagaagtaa tcaatttgat aaatgaaagc ttgatttgtt ccctttcgtt attcggtccg
    13501 cttgaaacaa gctggatgga tgtatcagca agcttagatt catctcgatc tgctttttta
    13561 aagaaataat tcaaatgata gccgaccttt ttttcttcag gtaccgagac gttccagtct
    13621 gttaagaagc gaagctggag caaagaagaa gcggcaccga aaatccgtat atgcgtatcg
    13681 cgccaatagc cgaatttttt tgttgtattt acatattgat tggcaatatt gaaaccaccg
    13741 gtataactaa tctttccatc gatcacaacg atttttcggt gatcatggta attcaatcgg
    13801 aaacgaagta atgccctttg tgaggtaaca aatgtatgga catgtccacc atttttgatt
    13861 agacgattga aatcttttgc ttttgtgcca tgagagccaa atgcatcata taatattcga
    13921 acttccacgc cttctgcggc tttttcttct aaaacatgta agacttgctg gccgatatta
    13981 tctgtcacaa atgcgtaata ttcgatatgg atcgaatgtt tggctttttt tagatcttga
    14041 agcagtgaat ccaatttctc ttgtccgtct gtgagaagag tgacagaatt cattcttgtc
    14101 agcggcatac gatttaatga agagaagaag tcaacaaatt gctgtttgtc tttatcgccc
    14161 atgtcaggat cgtaatgttc aatggtatct cctctaaaag actgaaagtt ttctaattct
    14221 ttcaagtcac tttgtcggag atagaatttt ttcttatccg ttaatccacg tccgaaaaat
    14281 aaatataata caaagcccac cccaggaagg gcaaacaata ctaagagcca tgcccaaatg
    14341 gctgctacat ctcggggttt gatcaaaata gccaccaaag caataagtgc atttaataga
    14401 taaagggcgg ttataatact agataccaca aaaatgttcc tccttacata caataacatt
    14461 agtatagcat gatagaagaa agaagcctac caaggtgaat atacttttca ctcctttcat
    14521 gaaaatgtag gtaaatcggg ctaaagtagt tgctttttgt aggttaataa ggtaggatat
    14581 tagtacagtt gaatatctcc ttaggggagt aaccggcaac gtttgttgcg ataatatcaa
    14641 cagtaagtta accaaatggt tttctggtat tgtatgaaat ggtgagacct aagacaaatg
    14701 cgtatttgtc ttaggtctct tttttttcag acaggactag ggccaagatc aaaaaatggg
    14761 ggatgaaatt tggaaaacaa ggaacaagca caacaagcgg tctataaaga aacgatcgac
    14821 tcgttgatgc agcaaacgca gagctcaacc gatggattga gcaccacgca agcgaaggaa
    14881 aaattagaaa gagatggctt gaaccagcta aaagaaaagc cgaagaagcc aacttggaaa
    14941 ttgttcttag aaactttcaa agatgcaatg gttatcgtat tgctgattgt cgcactgatt
    15001 cagatggtca tgggtgacta catcgaattc ttagttattt ttgcagtatt gatgttgaat
    15061 tctataatca gtgttgtcca aacaaaaaaa gcagaaggtt ctttagaagc gttgaaaaac
    15121 ctttcagcgc ccgatgctcg agtgattcgt gatggtaagg aaaaaacgat tccggcaaac
    15181 gaacttgtgg taggagatat cgtgttgtta gacgcaggcg attatgtacc tgctgatgga
    15241 cgtttactag atgcgggatc attaaaagta gatgaaggta tgctgacagg agagtcgact
    15301 tctgcagaaa aagaagtaac ggatatcaca agtacggtac caatcggaga tcgtacaaat
    15361 atggtattca gtggaacgat cgtaacttac ggacgcggta aattcttagt gacttcaact
    15421 gggaataata cggagatcgg acaagttgct ggccttttgg aatcaacaac tgagcaagtc
    15481 actccgttac aacgaggtct ggataaattc agtaaaaaac taagtttggc tattttggca
    15541 ttgtctttat tgattttagg tatccaactg tttcgtattt atcttggaga aggtacagga
    15601 gatatgactg cagatatcgt cagcgcagtg atgtttgccg tagccgtagc cgtagctgct
    15661 attccagaag cattacagtc tatcgtaaca atcgtgttat ctttgggaac gaataaaatg
    15721 gcaaaacgtc atgccattat tcggaagctt ccagcagttg aaacacttgg ttcgacaagt
    15781 atcatctgta cagataagac tggtacattg acacaaaaca aaatgacagt tgttgactac
    15841 tttttaggta atggcgatac tggggatttc acaaatgacc cttcaaaatg gacagctgac
    15901 gaacgtcgct tgatcgaaat cagtgtttta gcgaatgatg catcgatcag tgaagacggt
    15961 actaaattgg gcgaccctac ggaagtagcg tttatcgact ttagtgaaaa attaaaccag
    16021 ccttatcagg aaatccgaaa caactaccca agacaagcag aacttccttt tgactcagat
    16081 cgaaaattaa tgtctactgg acacacaatt gataacaaaa attacttgtt tgttaaaggc
    16141 ggaccagata tcgtcttcca acgcagtaaa aaagtattgc taaatggtga agtcgttccg
    16201 atgacggatg aattgatgaa aaaattccaa gagcaaaatg aagctttttc aacacgcgca
    16261 ttacgtgtac tagcttttgc ttaccgtcca cttgataatt ttgatttatc atttgaagat
    16321 gaagacgatt tgatccttgt tggtttgctt gctatgatcg atcctccacg tgctgaagtg
    16381 actcaggcgg taacagaagc aagaagtgca ggaatcaaaa caatcatgat tactggtgac
    16441 cacaaaacaa cagctgttgc tatcgcaaaa caaatcggga tttttgaaga aggaaacctg
    16501 gctttgaccg ggacggaact agatgaattg acagaagatc aactaaacga agttttagaa
    16561 aaagtcactg tttatgcacg tgtttcacct gagaataaaa ttcgtatcgt tcgtgcttgg
    16621 caaaataaag gtcatatctc agctatgaca ggtgatggtg taaatgacgc accagcattg
    16681 aaacaagcag acatcggaat tgctatggga acgggtacag atgtggcaaa agatgcagca
    16741 gcgatggtat tgacggatga taactttgct tctattgtca gcgcagtaga agtaggacgt
    16801 aatgtgtttg ataatatcaa gaaagctatt tcttatctat ttgcaggaaa tttaggtgcg
    16861 atcattgcga tcatctttgc cttagttgct gactggtcca atccatttac agcattacaa
    16921 ctgttgttca tcaatttagt caatgattca ctgccagcaa ttgcattagg tacagaaaaa
    16981 gctgaacctg ctacgatgaa ccgcccgcca agagatccaa attcaggaat ctttacggac
    17041 aaaacattga tttctgtaac gtatcgtggt gtgctgattg ggattgctgt tatctttgct
    17101 caatggcttg gcatgcagca ttcacctgaa ttcggtgtag ccatgtcatt ttcaacattg
    17161 atttggtgtc ggacattgca aacattccca gcacgttcaa acacacaaac agctatccaa
    17221 gcagggttct tctctaacag aagtgtctta ctagctgtag ttgtatgtag cgcgctttac
    17281 tgcttaacaa tgatcccagg actgaaagaa gtcttctcta tcccatcagc atttggattg
    17341 acagaagtct ttacatgtat gggaatcgca cttgtgacaa ttgttttgat ggaaatcact
    17401 aaattgatca ttgtacgagt acaaggaaat aaataaaacg aatcaacgag gttgtgagcc
    17461 tgccgtttaa ttctgagtca caaggtgcaa tttcaacaaa cagcttctca tgtttgttga
    17521 atattgtgac ttgtgaccga agagttggca ggagaacacc gtttaaggaa gaagttgtaa
    17581 aatcagcatt ttgagctaat actgagggtc atctgattgg acactgttta ttcggaaaga
    17641 gggactttga cacgactttg gtcacagtcc ctttttttat ttatgataga cggaaagcca
    17701 atcattttat tattcatcaa aagagataga atagataaaa gaggagaatg atttattccg
    17761 aaaagtgaaa acatataaca aaaaattaga ggaaatcaaa tgaatgaaaa agaatgggat
    17821 gaatttgctg aagaatatta tgaaatccag caagaatcgc aagtaactat tgctgaagat
    17881 gtaaaaaatt atctgaaaac ggagaaagtc ctgcctgctg aaacagcagc agacgttgct
    17941 ggaggatctg gcagatttct ttccttagca aaagaaatta atcgctatga tttgatcgat
    18001 ttttcttcag gaatgctaga aaatgcaaaa aaagaagcag aaaaaaatga gttgccaaat
    18061 gtttccttta tcaaacagtc gttagaagaa tttatccaat caaatgagac atatgaaatg
    18121 gtttttacag cagcaaatcc agcattggta catcttattc aattagaaaa attgatgaaa
    18181 aagtcaagca aatggtgcgt gataatcaga gtagtggaat caatcgatga tgttttttat
    18241 cctatcgaaa agcaactagg aatgatagaa gaagatccta atacatcgcc agttgtaatg
    18301 gatcaatttg aaacatactt aattgatgaa ggattcaaag tgaagaaaaa agattttact
    18361 tattttacac aggaatcaat tactgaagaa ttgatgaagc tatactatga cgcgtacgca
    18421 aaagatcctg ttgtgaccag gtacattcgc aaactattca gtgaaaaacg agaaatcctt
    18481 agtacgacta agctgaccta tagactgctc ctttggtccg cagataaaaa aagaaccttt
    18541 cacgactcgt gaagggttct ttttatgatt ttattatatt ttgtagtaac gaatatcatt
    18601 ttcgatcttt gtaatcgaaa aaggtacatc tgaagtatat tctgcacatt ctccgtgttc
    18661 aggaagcgtc tcactgaaaa tcgtttcata ttcgtcaaca gtgatccgtg ttcgagaatc
    18721 tagcatctct tgatgagatt gtgcgaagag atattcttga tagttttctt ccagataacc
    18781 tgtaaaaaat tcacttactg caccagaacc gtagctgaat agaccgatgc gatctcctgg
    18841 ctgtagtgat ttcgaatttt ccagtagaga agttagcccc aggtataatg aaccagtgta
    18901 aagattaccg attcgtcggc tgtaacggat gctttcttca tagcgagcca taagacgttc
    18961 ctgattttct tcgtctgttt ggtctaatac gctttgcaat gccttttttc ccatcttagt
    19021 atacggaata tggaaagcaa tcgcctgata atcttcgagt cctcgacccg acaattcttt
    19081 atgtcgattc caaacttttt ggaatgattc gatatacgta gaattagata aaggaccatc
    19141 aacaacagga aattcgctat aatctggacg ccagaaatca tagatatctt ctgtcagaaa
    19201 tacgctgtcg tcttcaatcg ataaaatacg cgggttttga gtgatcatca tcgcaacagc
    19261 accgacacct tgcgtcactt caccaccgct tgccaagccg taacgagcaa tatcacttgc
    19321 tatgactagt acttttcgtt ctggatgatt tttgacatat tctttcgcca tgtgcaaagc
    19381 tgcggtgcca ccgtagcaag cctcttttac ttcaaaagaa cgagcaaaag attggatttt
    19441 cagtaaatgg tggataatta ccgcactcgc ttttgaatga tcaatcccgg attctgttcc
    19501 gacgatgacc atgtcgatta gctcacggtc tttttctgtt acgattttgc tggcagcatt
    19561 tgctcctagt gtaatgatgt cttcatttgc acgattgact gccatttggt cttgaccaat
    19621 ccctatatgg tattttgcag gatcatcccc acggctttct gccaactctg tcatatctaa
    19681 atataaatta ggaataaaaa aggaaagacg atcaatccct attttcattt caacacctct
    19741 ttacttatga taaaaaactt actttactaa ggtatcataa aaagattaaa agaaatataa
    19801 aaaagagaat atttaaggat t
//
LOCUS       DAJSBL010000051        19476 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.051, whole genome shotgun sequence.
ACCESSION   DAJSBL010000051 DAJSBL010000000
VERSION     DAJSBL010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19476
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.051"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(22..996)
                     /locus_tag="ONT85_002109"
     CDS             complement(22..996)
                     /locus_tag="ONT85_002109"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HCR2379069.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            complement(1076..1951)
                     /locus_tag="ONT85_002110"
     CDS             complement(1076..1951)
                     /locus_tag="ONT85_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HCR2379070.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            2081..3988
                     /locus_tag="ONT85_002111"
     CDS             2081..3988
                     /locus_tag="ONT85_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family peptidase"
                     /protein_id="HCR2379071.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            complement(4529..7807)
                     /locus_tag="ONT85_002112"
     CDS             complement(4529..7807)
                     /locus_tag="ONT85_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HCR2379072.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEVPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLESYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(8078..8623)
                     /locus_tag="ONT85_002113"
     CDS             complement(8078..8623)
                     /locus_tag="ONT85_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HCR2379073.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDAVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(8635..9327)
                     /locus_tag="ONT85_002114"
     CDS             complement(8635..9327)
                     /locus_tag="ONT85_002114"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HCR2379074.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYKPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(9346..9630)
                     /locus_tag="ONT85_002115"
     CDS             complement(9346..9630)
                     /locus_tag="ONT85_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2379075.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(9643..10206)
                     /locus_tag="ONT85_002116"
     CDS             complement(9643..10206)
                     /locus_tag="ONT85_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HCR2379076.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            10434..11096
                     /locus_tag="ONT85_002117"
     CDS             10434..11096
                     /locus_tag="ONT85_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HCR2379077.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            11220..12416
                     /locus_tag="ONT85_002118"
     CDS             11220..12416
                     /locus_tag="ONT85_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HCR2379078.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(12517..13893)
                     /locus_tag="ONT85_002119"
     CDS             complement(12517..13893)
                     /locus_tag="ONT85_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR2379079.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(13930..14796)
                     /locus_tag="ONT85_002120"
     CDS             complement(13930..14796)
                     /locus_tag="ONT85_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose transporter GlcU"
                     /protein_id="HCR2379080.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(14885..15910)
                     /locus_tag="ONT85_002121"
     CDS             complement(14885..15910)
                     /locus_tag="ONT85_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HCR2379081.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(15928..16602)
                     /locus_tag="ONT85_002122"
     CDS             complement(15928..16602)
                     /locus_tag="ONT85_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR2379082.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(16786..17679)
                     /locus_tag="ONT85_002123"
     CDS             complement(16786..17679)
                     /locus_tag="ONT85_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HCR2379083.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(17753..19450)
                     /locus_tag="ONT85_002124"
     CDS             complement(17753..19450)
                     /locus_tag="ONT85_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HCR2379084.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
BASE COUNT         5684 a         4189 c         3445 g         6158 t
ORIGIN      
        1 ttctttccct cttttttttg tttattcgaa ttctttttct tccagacgga ataacggact
       61 catcggtgta ttttcatgga tgcgtttgac cgcttcaccc attaattctg aacatgtaac
      121 gattgttaag ttttctggat gacgattttc ttctgtgaaa acagaatcag tgatacaaat
      181 atctttgatt ggtgcattgt ctaaaatact tttcgcatga tcggacaata atccatgtga
      241 cgcacaggca tatacttctt ttgcgccgtt ttcttttaat acatttgctg ctcgtgcaag
      301 cgttgctcct gtgttcaaga tatcatctac catgatacat gtctttcctt gaacatcacc
      361 gatcacataa ccaccctcag ttgaagcatc atctgcatga tctacgatgg ctaatgtgct
      421 gttcaaatat tctgaaagac tacgtgcacg ctgaacaccg ctgttttttg gcgaaacaac
      481 tacgatgtca tctcccgtca gacctaattg acggtaataa tgagcaaaca acggcatcgt
      541 aaataaatta tctacaggaa tatcaaagaa tccttgtact tgaaccgtgt gcagatctaa
      601 agtcagtact cgagttgccc ctgcttcttc gatcaagtta gcgatcaatt tcgcagtgat
      661 cggttcatga ggtttcgccg tgcggtcttg cctagcatac ccatagtaag gcaatacgac
      721 attgatcgtt ttcgcacttg ctcgcttcat tgcatcgatc atgatcagta gttccatata
      781 gtagtcattg acaggtgcat tcgttgattg aacgatatac acatgatccc ctcgaatact
      841 ttcttcgata ttgatggaaa tctcgccatc actaaattgc ttcacgacgc ttttacctaa
      901 ttctgttccg aacactttcg cgattttttc agctaatgga cggttgccat tcaaactaaa
      961 aatcttcaac gtgtcatcct gatacttatt actcatgtag tcctccccaa aacttctcta
     1021 ttcttcgata gtttaacata tttttccaag attttctgtt aatttcagca aattcttaag
     1081 gaagtgaaac tgttccgtta ctccaactgg caggaatgac tttatgataa tcgccccagt
     1141 tgtcgtgtac tttgtaataa cgttctcctg tttcagtttc aaaataagcg tacgctgtca
     1201 cccaatgatt gccatatgta ctgccaagta ctttcaaaat accaatcatc acaggtttgc
     1261 cttcacgtat acgttttgta gctcgctgcc atgaacctag aagagttgct cgtgcacgct
     1321 catgattgcc attcttcttt aaaaaactag aaatccctgt actaacctga aaaggaatcg
     1381 taggaagtcc tagtggttgg atctggctac gcaatgcctt cgtcaaaacc aacgaatcag
     1441 ccgtattttt cttgcgaatc tcatttggaa gcatccaccc cttacgatag tcttgataat
     1501 aggcaaggag aactgctgcc gcgtaagtac cacaaagata gcctgagggg gtagcccatt
     1561 tcttatacct caaaaaacgt tcttctggca atcccaccca gcaatttgcg agttcctttc
     1621 ctgacctttt ttcttcgatc aaaacgggaa tatcttcttt tctgttgatc catctagata
     1681 actcgattgg ctcttcattg atcagcttcc attttttaca agaaagaccc tcttgataag
     1741 tatattttgc tggagaatat tctaccggat gtcctgattg atcgataatc acataacttt
     1801 gatcttcaaa aaccagagct aaaaaaagca atttactttt tcttgactgc agctgctgag
     1861 tgctggctaa ttttttaatt tttttatcta ctttgaaata taaaggttta tccactactt
     1921 gattcagttg ggatcgcctc ctttttttca ttataagtga aggattgaaa aaaacaaatc
     1981 agtttgattg ttcgaatttt caatttttta tgttctattt gttaattcct tcattttgta
     2041 agataatggg cacacgaaac aaaaaaggag cgatcgatga atgaacaaag aacgattgaa
     2101 acaagatttc tatgaagcag tcaatgaaga atggcaaaaa acagcaaaaa tccctgcaga
     2161 taaaccagct acaggcggtt tccaagattt agttgacggg atcgaccaac ttttgatggg
     2221 agaaacagac gatctgcttg cccatccaga aaaagtgtct tcagaaaaaa tgaaacattt
     2281 ccttgcatat tatcaactgg caaacgatta tgaccgaaga aatgagttgc atgctcaacc
     2341 gcttcaacct ataatcgaaa aagttgaatc attgcagtct ttcgacgaat tgaataagca
     2401 atttccagaa tggctgttgg atacattacc attgccattt aatctggatg tagatgcaga
     2461 tatgaagaat gcgcaaacga acgccttctt tgcttatccg ccttctttat tccttccaga
     2521 taagacgtat tatgcatcag accatcccaa cggtgcacaa ctaataggcg ttttctttga
     2581 tatggctgtt cagttattag agcttgccgg caaagaaaaa agtcaggcag aagcaatcac
     2641 ggaacaagca gtgcaatttg ataaattgat tgctcctcat gtaaaaagtg cagaagaaaa
     2701 cgcagattac agcaaaatgt ataatccgca atcttttgat acatttatta aatacacaga
     2761 taaactggac ttgaaaaaac ttgtcacggg tttgattggg acaactccag ataaagtgat
     2821 cgtaactgat ccagtgtact tcgatcattt gaatgacatc ctgacagaag ctcattttcc
     2881 gttattcaaa agctggatga tcattaaaac agtacgttct cttagtggtt atctatcaga
     2941 agaattccgc caagtcagcg gaatctattc tcgcgtattg tctggaacag acgaagcaat
     3001 gcctcagaaa aaagcagcgt attaccttgc ctctggacaa ttcgatcaag tagttggcga
     3061 ttactacgga aaaaaatatt ttggcgaagc tgctaaaaaa gacgtagaac aaatggtgaa
     3121 aaaaatgatc gccgtttata aaaaacgtct agaagaaaat gactggcttg gggatgcaac
     3181 aaaagaaaaa gccgtcataa aattaaataa cttaggtatc caagtaggct accccgataa
     3241 gatcccagca ttattcgacc agttcaaaac agtacctgca gatcagggtg gtactttgct
     3301 ttcaaatact cttgttttca gcaaactcac attaaaagat cgtttcagca aatggaacaa
     3361 accagtggat cgttcagaat gggaaatgag tgctgacacg gtcaatgctt attatcatcc
     3421 atttcggaat gtgatcgtct ttccggcagc gatccttcaa gcaccatttt acagtctaga
     3481 acaatcaagc tctgctaatt atggtggaat cggagctgtt atcgctcatg agatttccca
     3541 tgcatttgat aataacggcg ctttatttga cgaatatggc aacttgaaca actggtggac
     3601 agaagaagat cttacacaat ttcaaaacaa agcacaagca atgatcgaag aattcgatgg
     3661 tctgcctttt gctgacggaa aagtaaacgg aaaattaacc gtttcggaaa atattgctga
     3721 tgcaggcggt ctaagctgtg cattggaagc agcaaaaacg gaatcagatc tttctatcga
     3781 ggaattcttt gtcaattggg caacgatttg gcgtacaaaa gcaaaaaaag aataccaaca
     3841 acttttactg caaatcgatg tccatgcccc tgctaaactc cgagcaaacg tgcaaccaaa
     3901 aaaccttgac gaattctatg aaacatttgg tatcacggaa caagacgcta tgtatctagc
     3961 gccagaaaaa agagtgcgaa tctggtaaga ggttgtgcca gaagtggtca gcttcaagaa
     4021 ataagaagga atttgggaaa atcgcttctc aaatttttgt gacatttcgg cttatttttc
     4081 gaagaagtta cttctggaat accatgtatt agaggttgtg aaaaagctgc ttagtttcaa
     4141 gtagtaagaa aaattttgtg aaaatggctt tccacatttt tgcataaatt tgacttacta
     4201 ccgagaaact ggcttttgaa caccgtttat cgagaggttg tgaaatcagc gtcctgacct
     4261 gagtattaga gaaaaaaacg gaaaaatagt tcctcatatt ttgccgtttg ttgagctaat
     4321 accgagggtc agctgattga acaccgttta gtggagagat tgtgaaaaag ctgtcctgca
     4381 tcaagtaata agaacagcca atcaaaagca gcaaggagaa cctcatctat aaaaagatag
     4441 aaggaagtga gacatctatt ggtctcattt ccttctattt ttactttcct cagtcaattg
     4501 ttttacatca taaaaggaga aattccactt atttttttcg ctgtttcatg acataaacaa
     4561 ctacaccgat taacaaaatt cctccagcta atgctagcaa cgggttatta aattctcccg
     4621 tttttgggag tccttgagtg ccattttttt cactttcttt tgctgtccta tccggatgag
     4681 aaggttttcc atttggtttt ccatttggtg attcatttcc tggattttct ctttctggtt
     4741 tgtcatttcc cgggggcaca tcggcagttt ctcgcacagt gatcgctttt tgctgggtaa
     4801 cagattctgt atgagcagac catttttccc catcaaatat ttccgaacga taagtgactt
     4861 ctagcgtata ttctcctttt gaaggtgctg aaaaaatagc ggtatgatta gaaaattgag
     4921 cacttgactg tgtttcaacg atttggatct tttctgggaa ataacgcata tcttcaaaga
     4981 caggcgttcc ttgatttggt gtgaaagttg acacaaaaac tgtaaaaggc ttcagttcaa
     5041 tgatatttcc ttctgcattc tccgccacac catttgcttc ttgtataata atcggattat
     5101 tttgttcatt tggttttgtc tgatttggtt cttgtacgat tgcatactct ggatattccg
     5161 aagacggtga ggtttctccg tcaaaaatag cacctacaga taatggaaga ccatccactc
     5221 tttccttttc tgtttgacga ttccccggtt taatctgcat cagtccatga acatggatac
     5281 ttccattttc atagcgcgta acaggctgct gaccttcttt cgaacatgta tttagagact
     5341 cgatccaatc ttttgatatg gtatgtccaa gagtattttc tgattgtttc tctcgttcat
     5401 tccaatagta gttcaacgag tggtagattt gatcttcgtt ttgattcaat gggcggatct
     5461 gctctctcat ctcaagattc tctgttcgga aagataagat accagaagca tggtagtctg
     5521 tgtttcctgc tgaagaggta taaagagcca cattatatga accgttatta aaacttgtac
     5581 tgctattgac gataatatct gttccgctat tggaatcgat tcctttcgca agattttcga
     5641 aagacaaact attgatcaat tcatgagggc ccgttaaaga agaaccgccc attttgaatc
     5701 cattaccgtt accttctccc tcaattcctg gtacccagcc gtttcgataa gcaactgagt
     5761 ttttgatgat tacttttccg atcgaacctg tttcattttt agcaaaaagg tcccaaccgt
     5821 catctgcatt atgatacgcg atacaaccgt caaatacatt tccttctcct attgtcagtt
     5881 tggccgcaaa tccatcggca tcttcaaatc ctggatcggc attttcaaaa gatgtacagt
     5941 tcttgactaa gttatgcgcg ggccatgctt cgaaaggatc atttgagcta ccgctgattt
     6001 ggattcctgt atttccattc cgataagcat gaatctcttc gatcaaatta tgatttccag
     6061 aaatctgtaa tcctttttgc atagctgcac tatttgtaat gtcaaaccct cgtaaacccc
     6121 agtagtgact gaacagtgtc atcccatttc cagttccttt aaaatcaagt acaggacgct
     6181 tgttttctgg atttttctct gcaatcaact gaatcgggtt ttctcttgtc ccgttaacgc
     6241 cttttggaat cgtcaatcct ctttcgaagg aataagttcc aggcgccatg acgattgtct
     6301 gcccagcata tgcgtatctc aatgcttgtg tcagagataa tggattttgt ctgcttccgt
     6361 ttcctgcatc tgacccttct ggtgttacat aaactgtcga atatttaggt ttacggtagt
     6421 ccactgtatg aagaatctct tttgtttcat aactagtcat ctctacgtat tcttctggcg
     6481 gatagttttt atctggcgta aaactgaaag taaattcgtt cgttccttcc atcagttttg
     6541 tctgataatc aaattcccga ttcgcagtga tagccaagtt ttttgcgttc ttaagcacct
     6601 ctcctgttgc tttattttta attgtcaata atccgtcagc atttgctcta aaggacatag
     6661 tatgatcttg gtttcctgaa tgattactag aggtcacagt gtaaatcgga tcaatcaagc
     6721 gagtaggtct ttcttgccca ggaggatctg tcgcaggatc tgagtaagca acttcgattc
     6781 cttctacctt gatcgtcata ttccttgctg cagcaaatcc tgcataaata accgattcat
     6841 ctgaagcgta taatttttcc cagtcataca tgatgtcaga agccatttct tctcctgttt
     6901 caggatcgta ataatatgac tctaaaccag tattcgtttt tttcatctct acgtccagtt
     6961 tagtaatcga gttcgtggcc ggaaacaaat tcccatgacc gtttccgaat atattataag
     7021 aacctgtttc tttttccaag aaccctgctg aagtctctaa aggtgttgtt tccactgaga
     7081 cactcccagg agctggcgca gcttgcggat cttcagatga tattcctgtg attgcccgtg
     7141 tccccaatcc aagtcgcatc gtatatttgc tgccgctcgt gttgaccact tcttgtctat
     7201 ccgagtccca gacatattcg atccgactgc ctagtatggc aaatgaattt gaataaaatg
     7261 cctgcccaaa caaatgattt tttggtactg cgtctcttac cattaaagca aatccttctt
     7321 gggcattcgt ataggtccaa gattctattt cgatcgtcgc ccgcatccta aagttgatgt
     7381 ctttggggat tgcttgataa taataggtca ttccatctga tccgcttgac tggaattttc
     7441 cgccgttatt cgtggaagag agccgcacgc cgtcttctaa tggttcaatg gcatacctac
     7501 tagcacttgt gctttctccg aaaacaaccc ctttccactc ttcatttgct tcttcagatt
     7561 gcttttttgt cttttcattt tctgattctt tcatcatagg atcatttcct tcttccgtat
     7621 ccatatcttc actagttctt ggtacttcct ccattgttga catcccttca atgttcacgt
     7681 gtgcattttc ttcgttcaca gactgttgtt cgttagcagc aacttcaacg acagaaatta
     7741 aaaccggggt actgagcata aggagattca tcattagcgc actgtatttt atacgttttt
     7801 ttaacaagtg tattcctccc gtaacaaatt agaaagcgtt tacttaaagt attacctaat
     7861 ttatttttac tgtcaataaa aaacaaaatt ataataagtg tattttataa aaagaaaaaa
     7921 tataacttaa gttgttgata ataaagagat ttattaacgt aaaaagaata agttttttta
     7981 aaataacgta taaaaaagag cataaaacaa agtcgtttgt tttatgctct tcttttatta
     8041 ttttgtgctt tttattttat caaatcactt tgttttttta taaaatttct gcgcctagtg
     8101 tggactcaaa atgtctcaat gcccattcat gtccaacggg atcgaaactt gccacggcat
     8161 ctttgaaaat atggagtttg tatcctagat tatacgcatc gactgctgta tgcaacacac
     8221 agatatctgt acatacgccg gtcaaataaa tatctgtgat ctggcgttct cgcaaacgga
     8281 tgtctagatc tgttccacta aaagcggaat aatgccgttt gtctatccaa taaacatttt
     8341 cctcttgctc atagtcttgg tataaacttt ttaaagagcc gtacaattcc cgtccacccg
     8401 ttcctatcac attatgcgga ggaaataagc ggttctccgg atgaaaggaa tcttttggat
     8461 catgcgcatc aatggcaaaa acgacaaaat ccttttgatc aataaacttc tttgtatgag
     8521 cagtcagctt tttttcaatt acttgtcctg ctgtcccagt agtcagcttt ccatcctctg
     8581 caacaaaatc atatgtgtag tcaatcgaga tcaatgcgtt catcttagtc ctccttatac
     8641 gagatgtttg acaaattcga tcaccatttc tgctgatcgt ttgcctgcgt cttcgataaa
     8701 ttcgtcaaag ctttgtgtcg ccgagtgatc tgctgtgtcg ctcattgcac gtacgatcaa
     8761 aaatgggata ttgaactgac gagctgtttg tccaacagcg gctccttcca tttcgcaagc
     8821 taatgcttca gggaaattcg ttaagatttc tttgatttta tctggcgagt cgacaaatgt
     8881 atcacctgta acgatcaatc cttcttttgc gttcaaattc gttgcttttg ctgctttaac
     8941 catttcactg cgcaaatatg tgcttgcttc ataatataga ggcattcctg gtaattgtcc
     9001 tggtttgtag ccaaatcctg ttgcatctgc atcaaaataa gctactttgt ctgatataac
     9061 gatgtcacct acttgtaagc cttcaccgat tccgccagct gaacctgtat tgatcaccat
     9121 gttcacgccg tattgttgga tcaacagact tgtggtaata gatgctaaga cttttcctat
     9181 accggaacgt acgacgatca cttcgtggtt acctaatgaa cctgaaataa ataaagcgcc
     9241 tgcgcgttcc catgataatg gttcgcttag gttctctctc aaaattttta cttcttcttc
     9301 cattgcgccg ataataccga ttttcatctt tttaattcct tctttctata taaatgcgat
     9361 tgctaaaaaa accacacata acattaggat aacaataata agagatttca tcaaaaaaga
     9421 gttccatttg gtcgtctttt cccgttcgct gatccttgtt ttcgtgattg gtttattctt
     9481 tttgctttct ttacgataaa agctggcaat ctttttttct tcttgctgat aagctgtttc
     9541 tgcttttcgc tgttttttta atgactcgct tgcttgtgct tgttgtcgct tgcgaagttc
     9601 gcttcgcgta ataagcggtc ctttggccat aagccgatcc ctctactttc ctttggtcaa
     9661 tagttcccag tattgaatcg cataaatcgt ctttgcatca caaatcaact gatctttcat
     9721 cgcttgttgc gcctcttcaa gagttagatg atacaactcc agtacttcgt cttcatcttg
     9781 tgctaaagga ttctcgacct tctctacccc ttgtgcatga taaatatgaa ggacttcatt
     9841 tgcaaaacct ggtgaaagat acatcgaagt aagatggctc agcgacttcg cacgatagcc
     9901 agtttcttct tctaattctc tagctgctgt catttcggga ttttgtcctt ctcctggatc
     9961 gatttttcca gctggaatct ccaaaatcac tttttctaaa ggttttcgaa attgtttgac
    10021 aagaagcaga cgatcttgtt cgtcaaaagc aatgatcccg acaccaccag gatgaaatac
    10081 cagttctctc ttcgaagttc ctccatctgg caaacgaact tcgtctacag caacatcaat
    10141 aatttttccg ctatagattt ctttgcgact gattgttttt tcttcaaatt gctcattgtt
    10201 cagcatatct tctcctgctt tctttgtaat cataactgct tttttttcat ttttcaattt
    10261 ttctcaaaaa ctccttatta gtttaccata gataaacctt agattttctc taaatataga
    10321 aaatccttct ttagtccgat actctttcta gtagtccgta tgctaaaata agtatgtaat
    10381 cgaataacga aaagaattca atactcaacg ataaatgaca tggagggttt attatgataa
    10441 aacgaaattg gccgtggctt ctagcattgg tcttgctatt tctctttgtc cgaaacaata
    10501 gtttttcttt tgttctagga cttttattga taggtgtagt tttagttttg tggggactct
    10561 ttggttcgcg caggaaaaag ggaaaacaag aagaaattcc tgcactgact gacgaacttg
    10621 aaactcacta tgaaaaaagc ggtatgacag cgaatgaaat ttcatttttt cgacaaacaa
    10681 tgaatcaaac aaaaaatgag atcaatcaat tgcaaatgaa tatgcagcag acagccaaac
    10741 ttaaagcaat cgatctccgc catgatccag tcaaagcagc gaaagcttta ttcaaagaat
    10801 tggtgaaaga accaaaccga ctgcatgaag caagtcagtt tctttatacg catctgccga
    10861 atctagttga tttgacaaat aagtataatg agatcaatga ccatgagatc aaaaacaaac
    10921 agacctatga aaaactagaa gaaagcgcgc agatcatcga ccagctgtca gctttaatcg
    10981 cacaagatta tcaaaaattc gttgctgagg atctagatga tatcgacgta gagatatctg
    11041 ttgcgaaaca gagcttaaag cgtgataata aatatgaacc agaacaaaag gattaactct
    11101 tttgttcata aaagaagact cggaacattc agatgatgtc tggctgtttc gagtactttt
    11161 tctaaagtag agacattcat gtaagaatcg agaacagatt caacaaggag gaatttttta
    11221 tggatcataa accgactgaa acaaaagatg ttacaccagt taacgataca cttgaagatt
    11281 tattgaacaa cccgttttca actccaacag atacgctgac cacttcacaa caagcggaga
    11341 ttgatcagct ccaagagcaa cagacagctg ctcgtttaat tgacaaacta ccggcggaac
    11401 gccaagaaca agctaaacag ctagcagcaa aaatcgatgc taatgatgca cagtctgtca
    11461 tcagctacgg atcggctgcc caagcaaaat taagtgaatt ctcacaatcg atgttgaatc
    11521 atgtgcaagc acaagatatt ggtcctgtag gagattcctt aactgaactg atgtatcgtc
    11581 tccaagaagc caatcctgac gaactgcgtg ccggagaagg gaacatcttc cagcgtgtct
    11641 tcgggaaggt caaacaatcg atctatgaag tgacagcaaa atatcaaaag attggtgcac
    11701 aaattgacaa gatttcagtg aaattagata aagaaaaaga cggtctgcta aaagataatc
    11761 tgatgttaga acagctttac cagaaaaaca aagactactt tgatgcactg aatatttata
    11821 tcgctgctgg agaattgaaa atggaagaac ttcaaacaac gatcattcca gaagcgatga
    11881 aaagagcaga agaaactggc gatcagatgg atgttcagat tgccaatgat tacacgcaat
    11941 tcctagatcg tctggataaa cgtacgcatg atcttcgctt agctagacag atcacgatcc
    12001 agcaagcacc acaaatccgt ttgatccaaa acacaaatca ggccttggct gaaaagatcc
    12061 aagcatctat cgctacagcc attccgctgt ggaaaaacca agtcgtgatc gcattgacct
    12121 tgcttcgtca aaaagatgca gtaactgctc aacgccaagt ctctgaaaca acaaacgact
    12181 tattgaagaa aaattcagaa atgctgaaaa tctcagccat cgaaacagcg aaagaaaatg
    12241 aacgtggaat cgtggatatc gagacccttc aaaccactca aaatgatttg atcgaaacaa
    12301 tccaagagac attgcgtatc caaaaagaag gaaaagaaaa acgtcgtcat gcagaagtag
    12361 agcttggaca catggaagaa gatctgaaac aaaaactgtt ggatttaacc caatagatca
    12421 cctttcgctt gttactaaaa caaacgccca acaacagttt attacaactg ttgatgggcg
    12481 tttttcttat ctttcttcta acttctccat tggagtctat ttctcattca tcttgctgtg
    12541 tccatagcca tagccaaaat aaatgatcag tccaataacc attacgatac cgaacacgat
    12601 ccatgtcaca gtttgcagac gaagcatcaa tacaatacaa gtcaagatgg agacgattgg
    12661 caatagcgga acgaatggca ctttgaattc tccagctttt ggtttgccaa acatttttct
    12721 caacttcagt aagcctagac taacaagcat caaataggcg agcgccatga tattcgttag
    12781 ttccgctaat acattcaatg ggaaaaatcc ggcaaagaat acagctgcca caccagcggc
    12841 ataagtcgct tttttcggtg tacgatgctt ttgatcgatt tctgtcataa acttaggaag
    12901 taagccatcc ttgctgacag caaacagtaa acgagcaagt gaatacatca tcgagatcgt
    12961 taccgtcagt agcgttaaaa ttgcccctac agcgatcacc gcgcccactg ttccatgtcc
    13021 tacatacctc attgcaaaag ccactgggtc attgactcct agttcagtat acggaacgat
    13081 tcctgtcaag atcagcgtca cgatgatgta aaggacagta gcaatcagta tcgaaccaat
    13141 gattcctctt ggcacatctt tttgcgggtt tttgacttcc tcggctgcca ttgcaacggc
    13201 atcaaatccc aagaaagcaa aaaacacaag agctgcacca tctagcacac cggaaaaacc
    13261 aaaaggcatg aatggctgcc agttatcagg tttaacgaaa aatattccta caatgataaa
    13321 caacgcgatg ataccaaatt tgacatatac catggcatta ttcaaacgta atgctttctt
    13381 ggcttcaagc gacacgatgt aagtgacgaa tatcactacg agcaccgcaa tcaagtcaat
    13441 gtatgtacca ttttccggat tgtacccgcc agttaatgct tgtggaagtt cggcccctaa
    13501 acttttcaaa aaaccttgca tatagccaga ccatccggaa gcaacagagg aaaccgctag
    13561 taaaaattcc ccgatcaaca accaccctgt cagccaggca gcaaattcac caaatacaac
    13621 atacagatag gcatatggcc caccaatcac tggcactcgc gaagcaaatt ctgcgaaact
    13681 tagtccagac aaaatgacaa cgattgcagc taagacaaat gaaagtgaca gtgctggacc
    13741 agcattttga ttggccgcaa cccctgtcac cacaaatatt cccgtaccga tgatcgcacc
    13801 aattccaagc atgatcaaat cagacgtttt tagttctttt ttcatctcac tcgtatgatt
    13861 gagattgatc tctttcttac gaaacaaatc cattgaagtt cctccccatt ttaggttgga
    13921 aaacagcgat taaactgttt tcatataccc taaaaggata ccacctaaaa tgactaaaag
    13981 gcaaccaaca ataacataaa tgagctcttt tttcgttttt ctttcgccaa ggatgaaaat
    14041 cccacctaaa gtagagataa tgatccccat ttgtgaaagt gagaagccaa cagctaaacc
    14101 aagtgatttg actgttagaa gcatgcagat gtttcctaag ccccataata atccacagat
    14161 catgtttttc cacacaaact gatcgacttt aactttacgg aaagcaaaga agcttgcgcc
    14221 taagatcatt ccgatacttt gaggtaagat aattgacatc gcatccaaat tcgcccagtt
    14281 gacgatgatg gtatatacac cataaccaat cgtagagaag atcaatgctc taaaaccctt
    14341 accaaaatca agcattttat tatctgtatc tatttttcct tcgtcatctt gtcgggcagt
    14401 caaatacgca ccgcttacaa gaagagctaa agcaataata ccgtatacat aatctttcgt
    14461 ttgtgtccat tcatggaaga aaacggctcc cgcaatcgta ttcaaaatta attgaaaacc
    14521 agtagacaat ggtagcccta cagaaacacc catgtacttc attccatgga actgtccatt
    14581 ttgtccaaca ctccaagcta agccggataa aaatccgatc aagaaaatcc aaggggtgat
    14641 tacaggacta acaacgaaaa agacaactaa agaaaacaga aatgcaccga tcgtcattcc
    14701 tagtgtttgt tgattggcat cgccaccaat tttaccactt actaaaccaa tactacccca
    14761 tgccaacata gggactaatg ctattaaaat ttccataacc aatctccttt tatttcacat
    14821 gataagaaac ttatcagcga aaacgttaac aacacaagca ctttttttcg tttctaacga
    14881 ttttctaacg tttttcatta taaaaattta agattgggaa aaataaagtc attgttgtcc
    14941 cttttccttc agttgattca gccgtcaaat ggatacctaa tttttcggcc agattttttg
    15001 ctaaataaag tcctaatcct gttgaatgtg ttttgctaag tcgaccattt ctcccagtaa
    15061 atcccttatc aaagatccgt ttcaagtctt ccgctgggat accgatccct gaatctttaa
    15121 ctgaaaggct gactccttgc gcttcctttt ctatgctcac aatgatgttt ccgtgatccg
    15181 gcgtatattt gatggcatta ctaagcaatt ggttgaagat aaaagcgatc catttagcat
    15241 cggtcaaaac agtatgatcc tcaccaacga tctcgtagcg aatatttttt tggataaaat
    15301 aatttgcctg actgcgtatc acaggctgaa tgattccttt tagtccatat tcttggatga
    15361 ggtagtcccg cgagaaactg tcgagtcttg cataatacaa aacttgttct acgtattcat
    15421 cgatttttga taattcattt tctagtaatg tatacttttg atcgtcaata tcgaactcga
    15481 tcgaacgaac tagcaattta gaagcggaaa gtggcacttt gatttcatgg acccatgagt
    15541 caatataatc tttttggtct tgttgattac tgattgctga ttgcattact tcttggtgtt
    15601 cacggatcag ctgattgata tattcttcta ccagtttttc ttcttctgtc ctcccaccac
    15661 tcaaataatt ttgcaaaggg gaagtttttt gtatgtcggc taattttccc caccatcttc
    15721 tcttgctcaa gtaaccaccc agcagataaa aaatcagaaa gacgccctca atcaaagcta
    15781 aataagcaat tgtgctcaaa tcaactttga tgtttggcgc cagccacatc acaaagatcg
    15841 tcaatgcaat aaaaaacagc catcccaaga tcaatgtcca ctgatctttc aaatatttaa
    15901 aaaaattcat cttaaccgct catcctttca gggaacgata tatccctgtc ctacttttgt
    15961 ttcgatatat ccttggatac ctgcctgttc gatttttcgg cgtaaccgat tgatgttgac
    16021 tgtcaatgta ttgtcatcta caaatcgttc gtcatcccac agtgctcgca gcaatttttc
    16081 ccgacttaag atctttccat tgtgcttcat caaaataaat aatagacgat attcattttt
    16141 gctcaaatca atgatctctc cgttgatttc catacttccg ttgtcaacat tcaacgtaat
    16201 gccattgtgg ctcatcattt cactagataa ttctgtgtag ttgtaagaac ggcgaagtaa
    16261 tgcattgatt tttgcaatca gtacatcgat ttggaaatgt tttgtcacaa aatcatctgc
    16321 ccccatgttc atagccatga tcatatccat attcgtattc ctgctggaga taaaaatgat
    16381 cggtactttc gatacttcac gtattttttg acaccaataa tagccatcca aaacgggaag
    16441 attaatatct agtaatacaa gctgtggttc ttctctttca aagtctgaga acacttgatt
    16501 gaaatcggtc gttccaaagg tttcaaattg ccatttctca agttcctcca tgatcaattg
    16561 tcgaatgatc tcttcgtctt cgactaccat gatttttgcc ataaaaaatc cctccatctt
    16621 tcttcttgct actacaatat caaagaagcc cttgtgaaac aagttggctt acctggaaaa
    16681 tgtcatttta gcaaacgtta aaaaaaactg gaaatatgcg atttaatcgc gtttgtctct
    16741 tccaagcagc ttaaatttat tatgttattc ttaataattg gtctattatc tgttattttt
    16801 ctttcgttgt ttttgtcttt taaattttcg ataacaccaa taaccgaaaa gcgttagcac
    16861 tagcaatata cctagaagga gtataagtaa tcgataaaga tggtattttt ttgtctgttc
    16921 tatctttgaa gagacttctt ctgtaaaggg aactcgtctt cctgtcacta gtaatcggtg
    16981 agtgttcacg gtgtaaggtg tacaagtgat aagtgtgact agatcctgct tttctctaat
    17041 tgctaatgta tttgtttttg ttggaaggac aacaattttc tcaacgactt cataagcgag
    17101 catccgacca tacacttcaa gatagaatcg gtcatcttgc tccatttctt caagatccgt
    17161 aaagagtttt ttctcagcaa taccgctatg ggcggtaatc acggaatgtg tactctctcc
    17221 tccagttgga aaagaggtgc cttgcaggac tgtcgctcct ctatctaaca aaagatcatt
    17281 cgtttcatca aataaaggca agctaacatt gattttcgga atatagatgg ctccgatcat
    17341 atgttgttcg taatattctt ttcctggatt ttggacattt ctaaccgctt gctcaaaagg
    17401 atctttgact tgtcccattc ctgggattac tgatgtatga gcaagcgtct gatttttctt
    17461 ttcatgcact gctaattgtt gctcatgttt ttcttcttta tcgcgttcaa gctgcttttg
    17521 ataattctcg atccttcttt gatccaaata attgctgaga gcatctgcta aaaatggata
    17581 tgtaaaaact gcagctccgg caagaaacat cactgccata aataattgta ataaaagact
    17641 gatttgggtt tgaggtctat tacctatttt ctttttcatt ttcttcacct tcttatttaa
    17701 aaaaactgca gcaaccttta taaaaccgat tgctgcagct tatttttgtt gcttatactt
    17761 cagacttcat tttagattta cggtaccagc tgtacgctcc aatcatcaaa cttattccga
    17821 ttagtaagaa tgccaagagc ccgttcccac cagtagcagg aagtgacccg cgtttcatat
    17881 tcggtactct ttctggccct ggattttcta ctcctacgat ctgaatttct tcattatagc
    17941 ttccataaga gatgataaat tcagtaaagg ctgcctcacc tgttggaagt acataacctt
    18001 ctggcgccat ggtctctttc atttgatagg ttccataatc taacccttga acctcaaagt
    18061 atccatttcc atctgcgaca aattctgttg cttgtccgct tgttgcccac tcaattctat
    18121 ctgctcctga ttcataaaca gctaaaggcg actccccttt ataaaatatt gcgtattcag
    18181 aacggtcttc attccacaat tcgaatcttg catctttaag caattcactg cttgatgcat
    18241 catatttttc aaactttctt ccacctgttg tgactttctc tggcggctct actccttctt
    18301 cataatcaaa ttcatcattt cgtccgattt caataaccgc tgtgttgtta tggaaatcat
    18361 ccgcttctag ttctgtggaa accttcaaat tatacgtgat cgttaaatct cctccacgtg
    18421 catctgctaa tctacctaaa gtatctactt tggctactcc attttcttct gtcaaaatcg
    18481 tcacgacaaa cccgttgctt actacttcta ctatataatc ttgtcctttt gttaaaatcg
    18541 tctcgccggc tcgcacttct atttcctgat cataataagt tagaccaact gtaggcgtat
    18601 ctgtgatttt gtaatttttg ataacagcgc ttccgttttc aaggacagaa tcgatgaaat
    18661 aaggaatagg tgctgtgatc gtgtaagaag ccgtttttcc tctttctaga tcaatataag
    18721 aaatcgtttc ttgttctcca gcttcattaa taatcgttac ttgtctaagg tcgctttcct
    18781 ctacattcat ctgtttatct gcttctctga tttcgttttt cggatacaaa tgcacatctg
    18841 tattgtatgt gtctttccat gtagaaccat cccacacgcc atccaccata tcaggcatca
    18901 tcactggcat cataacaacc attggagacg cgattctgga aatatgagct ggcgaataag
    18961 tttctaagaa aagatagact ttatctctag aagaaggtgc ttcttccgtc acttggacgg
    19021 tagagaatac cgcttctcca gcttcattcg ttgtttgttt gtcaatactt gtcagtcgat
    19081 attttaaaaa ccaactttct gtgtgccgtt tttgaatcca ttcaatcgct tcggcagaat
    19141 taagaccgtc ttctcgttcc ggtgttaaag gattatctcg aatcaaatcg taatatactt
    19201 ccgttacgtc aaacaaatca aaatcgactc ctggaatatt ttcttcacca aaatccgtag
    19261 aaaccaatcc gctattcggt cgttcttccg gaacagatga gaaacctttt ttgtgtaatg
    19321 taatcgtcac ttcacttggt ttttgtgcga cttcttcggc tttgactagt tttccgttcc
    19381 cgaaaatccc tattcccgca gtaacgagta acagtacact cattcccgtg acaatcattt
    19441 ttttattcat ttttttctcc ccgttctttt tttatt
//
LOCUS       DAJSBL010000052        18740 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.054, whole genome shotgun sequence.
ACCESSION   DAJSBL010000052 DAJSBL010000000
VERSION     DAJSBL010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18740
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.054"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            256..531
                     /locus_tag="ONT85_002125"
     CDS             256..531
                     /locus_tag="ONT85_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2379085.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            544..2616
                     /locus_tag="ONT85_002126"
     CDS             544..2616
                     /locus_tag="ONT85_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HCR2379086.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKRVQ"
     gene            2781..4667
                     /locus_tag="ONT85_002127"
     CDS             2781..4667
                     /locus_tag="ONT85_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HCR2379087.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            4679..5626
                     /locus_tag="ONT85_002128"
     CDS             4679..5626
                     /locus_tag="ONT85_002128"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HCR2379088.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            5646..6173
                     /locus_tag="ONT85_002129"
     CDS             5646..6173
                     /locus_tag="ONT85_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HCR2379089.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(6236..6889)
                     /locus_tag="ONT85_002130"
     CDS             complement(6236..6889)
                     /locus_tag="ONT85_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HCR2379090.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            7021..7863
                     /locus_tag="ONT85_002131"
     CDS             7021..7863
                     /locus_tag="ONT85_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HCR2379091.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            8021..8917
                     /locus_tag="ONT85_002132"
     CDS             8021..8917
                     /locus_tag="ONT85_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HCR2379092.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            8920..9633
                     /locus_tag="ONT85_002133"
     CDS             8920..9633
                     /locus_tag="ONT85_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HCR2379093.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            9653..10171
                     /gene="msrA"
                     /locus_tag="ONT85_002134"
     CDS             9653..10171
                     /gene="msrA"
                     /locus_tag="ONT85_002134"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HCR2379094.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            10168..10395
                     /locus_tag="ONT85_002135"
     CDS             10168..10395
                     /locus_tag="ONT85_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HCR2379095.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            10547..11098
                     /gene="lepB"
                     /locus_tag="ONT85_002136"
     CDS             10547..11098
                     /gene="lepB"
                     /locus_tag="ONT85_002136"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HCR2379096.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(11181..11681)
                     /locus_tag="ONT85_002137"
     CDS             complement(11181..11681)
                     /locus_tag="ONT85_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HCR2379097.1"
                     /translation="MNARLLSTSALLLALDVILYKIAIGPAYFQITFGFLSMALMGYL
                     YGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLFLYHKKINLPSVIAA
                     QLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQIMVVYSFIKTLEARK
                     VFKRAV"
     gene            complement(11960..12358)
                     /locus_tag="ONT85_002138"
     CDS             complement(11960..12358)
                     /locus_tag="ONT85_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCR2379098.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            12618..13478
                     /gene="ylqF"
                     /locus_tag="ONT85_002139"
     CDS             12618..13478
                     /gene="ylqF"
                     /locus_tag="ONT85_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HCR2379099.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            13471..14238
                     /locus_tag="ONT85_002140"
     CDS             13471..14238
                     /locus_tag="ONT85_002140"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HCR2379100.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            14294..15151
                     /gene="dprA"
                     /locus_tag="ONT85_002141"
     CDS             14294..15151
                     /gene="dprA"
                     /locus_tag="ONT85_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HCR2379101.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            15273..17351
                     /gene="topA"
                     /locus_tag="ONT85_002142"
     CDS             15273..17351
                     /gene="topA"
                     /locus_tag="ONT85_002142"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HCR2379102.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            17320..18696
                     /gene="trmFO"
                     /locus_tag="ONT85_002143"
     CDS             17320..18696
                     /gene="trmFO"
                     /locus_tag="ONT85_002143"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HCR2379103.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
BASE COUNT         6518 a         3231 c         3899 g         5092 t
ORIGIN      
        1 ttttgtcgca gccccatttt tttgttgtga taaaagaaca agaaaggcag gaatgggaaa
       61 gtaaacatcc ttttgtcaga aacttttatc tttcttaagt tttacttgat atctgatact
      121 atttggaata gaatgaggcg ttgaaaaatt tatggaaatt tctaactagc aataaacgat
      181 gaaacaatga aatatttttc atccaaaaag aactttgcta taatgatgga agtttgtgta
      241 taggagatct ggaaaatgag agagagtttt gaacaacaaa aaaaactgtt gcatgatcgc
      301 tatggcgccc tttcgatgga tgaccgccgt caaatacttt gtaagttgcg taaaagaaat
      361 atattgatgt atcgtcagtt ggaacgtctg aaacatgatc tgcttcgttt ggagtcaaaa
      421 cgtgtacagt gtgaattaga ggggaaccaa acacaggttg aagtagtcga aaccaaaatt
      481 ttaaagaaaa aagaacaatt tttaaaaatg cttacacaaa ataaaaaata aaggggatgg
      541 acgatggaat ttgctgagtt agttattgta tttgcagtaa cgatcacctt atcgaacata
      601 gctagccgca ttttgccaat gattccagct ccactgatcc aaattttcct tggagtggta
      661 ttaggtctga cagagtgggg acaatcaatc gattttgaac ctgaattgtt cttagttatg
      721 attattgcac cattgttatt tagagaagga gaaaaagcag atatttcttc gattctcaaa
      781 aatttcggga caattttgtt cttggcattt ggaggagtca ttttgaccct tgtcggggta
      841 ggtgcgacgt tatcattcct gttgccaagt gtcccacttg ctgcctgctt tgcttttgga
      901 gcagcgttag gtccgacaga tgcggtagca gtcagttctc tttctggacg agtgaatatc
      961 cctaaaaagg cgatgcatat tttggaagga gaaggtcttt tgaatgatgc atctggtgtt
     1021 actgccttcc agtttgctct tggtgcgctg atcaccggat ctttttcagc agtaaacgcg
     1081 gggatgagtc tagtagtttc tagtattggg ggagctttga tcggcttttt gcttgtctgg
     1141 ttcaaacaaa aaataattca tttgatcgaa aaagcatcag cacaagatgt cacagggtat
     1201 ctgctgatcg aacttttgct tccttttcta gcctatgtat tggcagaggc atttgatgta
     1261 tctggaatca ttgctgcagt ggcagcaggt attctccaag cttctggttt taggaaaatc
     1321 tctgtgtttg atgcagaatt gtcgagcttg tcccacagta catggacaac tattgctttt
     1381 acactgaatg cattagtatt tatattttta ggaatcgaat tgacgcaagt gttctcgcca
     1441 gtatggggag acggtttata tccaaacggt cttttattag cgattatcgt attaatctct
     1501 gtcatgcttt ttgtgatcag attcatttct atcagtcttt tttatgtatt caaagatggc
     1561 agtaaaaagt tcaaaaaaca gttgaacgag atcttgatct taacatttgg cggtgtaaag
     1621 ggaacagtca gtttggctac gatttttatt ttgccatttt caatcaataa tatgatgttt
     1681 taccagcggt ctttgctttt gtttctgacg gcgggtgtca tcttagtgac tttggtcata
     1741 gggatcattg tcttgcctat gctgacggaa acagaggaag cgaaaagcac ggatttgaat
     1801 gctttgatga ttttagaaga ggtcgttgaa gttttgcgaa aagaaataaa ggaaattgat
     1861 cgtaatacaa aagaattttt agccactgaa gcagtgatag aaaattatca agaacggata
     1921 cgtgatctgt acttggaaga tttaaccgat gatgaaaagc aagaagtaca agaaatacaa
     1981 gcactgattt tgtcgatcga acgggatgga ttggatgaaa gttatcgttc gggcaagctt
     2041 tcttctaatg gttatcgttt ttactcccgt tttctttctc gattcgaaca ttctatcaca
     2101 agccagattt tgtcttttat cggcttttgg ttcattgttg tccgtaggtt aatgcgaatc
     2161 gttctccatc cgaaaatgtt ttggcaaaga agatatgctg atcgtcagac atttattagt
     2221 gatgaagata tccaagaaat cagagagaca tatcaaaaaa atactcagtt gattattgaa
     2281 agtttagata acttgactga tatttatgat gaaacgatgg ttcaattttt tatccagcaa
     2341 cgcaaacttg aaggaataaa aatgaactcg gggaatttga tttcttcgtg gatgatccag
     2401 caagatagtc tgtttacgaa aaaaatgcta agaggttatt atcttgaaag aaaaatcatt
     2461 gatgaatacg aagttgctga aaaaatcacg acgttttctg cgaacagcta tcgcaggaat
     2521 atcaatttgt tagaatctta tacgatgaac aaaccatctg ataacttttc tttccgattt
     2581 gcgtttcgag caaagtcaaa aaaacgagtc caataaaata aaaaggtaaa aattttcaag
     2641 ttttataaaa aacaaaacat atcagaacag gatcgaatcg gtcctgttct ttttttgaga
     2701 acacaaaaag aacaaatacc aaaaaaattt tcctgaagtt cactattttt atgatacaat
     2761 atccaagata aggagggaac atgttgaaag aattaaaagt aactgacctc aaaaaaactt
     2821 atggtgaaaa agatttgttt gaccagattt cttttttggt ccacgaaaaa gaccgtatcg
     2881 ggttgattgg gacgaacggg acgggtaaaa caagtctctt aaatattatt gccggtatcg
     2941 atagcagcga cggtgatcgg caaacagtgt tttatccaac tgattaccgt attggttatc
     3001 tttctcaagc agctgaattt tccgatgaat taactgttct ccaagcagtc tttcaaggaa
     3061 acagcccctt gatccaaact gttcgagcat atgaggaagc attgatcgaa ctaggggaaa
     3121 acggagaaga ccctgatgtg caaaaacgtt atgctaaagc agaagaacaa atgaataaag
     3181 aagatgcttg gactacagat accaacgcaa agattatttt gcaaaaatta ggaatcagtg
     3241 aattagataa aaaaatcagt actttatcag gtggtcagaa aaaacgagtt agtctggcgc
     3301 aagtgttgat tgatgaaccc gatttactat tactagacga accaacaaac catttagatt
     3361 atgaagcgat cgagtggttg gaaaactatt tgaaacaata ccgtggcgca ctattgatgg
     3421 ttacccacga tcgttatttt ctagatagag tagcaaaccg aatcttcgaa ctttcatttg
     3481 gaaaattata cgagtataaa ggaaattatg aagcttacgt cttagaaaaa gcagaacgtg
     3541 atcgtgtaga agtagaacaa gaagaaaagc gcaagcggct ttataaacag gaattagctt
     3601 ggatgagggc tggcgtacag gcacgtggca ccaagcagca agcacggatc aaccgtttcg
     3661 aagatctaaa ggagaacttg taccaagtaa accaagaaga tgatttagag ctgaacctag
     3721 cgacacaacg tttagggaaa aaagtactgg aaatcaaaga tggcagttac cgtataaatg
     3781 accaaacact gctagaacat ttagatttat tgattcaatc tcgggaaaga ctaggtatta
     3841 ctggtaaaaa tggtgcggga aaatcgacat tgcttaatat tctagctggc agaattcccc
     3901 tggatagcgg tacaatgtct atcggtgaaa cggttcatct tgcgtattat acacaagaaa
     3961 atgaagaaat ggcaccagat aaacggatga ttgcttactt acaagaagct gctgaagaag
     4021 caaaaacagc agatggcagt caaatcggag tagcagaatt acttgaacga tttttatttc
     4081 caagatttat gcatgggaca ctgataggca aactttcagg cggagaaaaa cgccggttat
     4141 ttttgttgaa actgctgatt cagcaaccta atgttttatt attggatgaa ccgaccaatg
     4201 atttggacat agcaacattg acgattttgg aagattattt tagaagtttt ccaggtgctg
     4261 tgatcactgt ttcccatgac cgttatttct tggacaaggt agcagataag ttattagtat
     4321 tccaaggaaa cggaaaacaa gaactttatt acggaaacat gtccagttat ttactgaagc
     4381 aaaaagagac ccagcagcct gctgaaaaag cgaaaccaaa aatacagagc aaggaacctg
     4441 ctgaaaagaa gaaattatct tacatggaac aaaaagaatg ggaaacgatt gaagatgaga
     4501 ttgcagaact tgaagaaaaa atctcattgc tccaagaaga gatgaatcat caaggagata
     4561 atttcactcg attacaagaa ttgcaaaacg acgtatctga aacagaagca caacttgaag
     4621 aaaaaatggc acggtgggaa tacttgagtg aatgggtaga agattaggag gaaaaaggat
     4681 ggaacaagcg tatcttgact taggaagaaa attgttggaa gaagggcatc gtaaagaaga
     4741 cagaacaggc acaggcacat taagcttatt cggataccaa atgcggtttg atctgcaaaa
     4801 agggtttcct ttgttaacaa cgaaacgagt accttttgga ttgatcaaaa gcgaattgct
     4861 ttggttttta aaaggagata cgaatatccg ctatctatta caaaataaaa accatatttg
     4921 ggatgaatgg gcatttgaac gctacatcaa aagtccagac tataatggac cagatatgac
     4981 tgattttggc catagatgtt tgacagatcc tgaattcaat gaagaatata aaaagcagag
     5041 caagcttttt tgcgagaaga tcctaaccga tgattccttt gctgaaacat acggcgatct
     5101 tggacacatc tacggctatc aatggcgtca ctgggaaaca aaagacggtg gctttatcga
     5161 tcaaatcaaa caagtgatcg aagcaatcaa aaagactcct gattctagaa gattgatcgt
     5221 ttctgcatgg aatccggaag atgttcctag catggctttg cctccttgtc atacgatgtt
     5281 tcaattttat gttcaagagg gacggctaag ctgccaattg tatcaacgca gtggggatgt
     5341 gtttctaggc gttccgttca acattgcaag ttatgcgttg ctgactcacc taattgcaca
     5401 tgaaacaggt ttagcggttg gcgaatttgt tcacacatta ggagatgctc atctttatat
     5461 caatcatatc gaacaaatga aagaacaatt gagccgagaa atacgcgcat ttccgacttt
     5521 agttttgaat caggaaaagc agtcagtatt tgattttgag atggaagata tccaattaga
     5581 aggatatgac ccacatccgg cgatcaaagc tcctatcgct gtttagaatc agaaaggatg
     5641 agaaaatgtt agctgcaatt tgggcacagg atgaaaacgg attgattgga aaagaagacc
     5701 gtcttccttg gcgtttgcca aatgatctga aattcttcaa acaaatgaca gaagcaaata
     5761 cactagtgat gggacgaaaa acattcgaag ggatgggtag ccgtccgttg ccgaatcgtc
     5821 agacgattgt tttgacgaga gattcttcct ataaagcaga aggcgtgcat gtcatgcata
     5881 gtgtggaaga cgtgcttgct ctggaaaaag aaacagacgg tatctttttc atcgctggag
     5941 ggtcagctgt ttatcaagaa tttctgccat tttgtacgat attataccgt acagtcatcc
     6001 atcatgtatt cgatggagat gcttattttc cacctgttga ctggtctgat tggtcactga
     6061 tcaatactag tcaaggagaa atggatgaaa aaaatcgtta tccgcatcaa tttgaaacgt
     6121 atcagcgtaa tgagaatcct gtaaaaagcg ggacaggcga attgacgata taaaaaagct
     6181 atggatagac tagctgttat ttagagctgt cccatccata gctttatttt ttttattaag
     6241 taaagaataa aacagagaag tacataaaac ctgcacctaa cataacaaac aaatgccaaa
     6301 caacatgcat atagcggata gaattcaagc tataaaagaa tgcgcctatc gtgtaagaaa
     6361 gcccgcctgc taccatcaaa ataacaccag tcattccaag agagtgataa agaggaacag
     6421 cagccacgac acagagccac cccataaaaa tatagatgat cgtagatact tttgatactc
     6481 tctcccgttt atgcaacgtc aacgctttat atataatgcc aagaatcgca aacaaccaga
     6541 ttaatacaaa taaagtccaa cctagccatc ctcggatact cagcaaacaa aaaggagtat
     6601 aacttcctgc aatcaaaaga aaaatcgaac tatgatcgaa tacttggaac accttttttg
     6661 cttttgtaaa aatcaatgaa tgaaacaaag tagacgttaa aaataaaaga atcatcatcg
     6721 atccatagat agcataagag acaatatgga gggaggaatg caagtgggca ccttttacta
     6781 aaagaaccac tagtcccgcg atactcaatc cagtcccaat tccatgggtg actgcattaa
     6841 acacttcatt taatatttca tacgttcgat taaatttcgt tttttccatg aaaacggctc
     6901 ctttactagg tgtaattaat gaaaaatcat tatttgtctg ttatactaaa aatagaaatt
     6961 gtcttacatg tagtttaaca tgaataaggc atcttgttaa agaaagagag agagaaaata
     7021 atgacaaatg taaaaatcgt aacagattct tcatgtacga tggaaaaaaa tgtacgcgat
     7081 gctttaggca ttcgtgtcat gccgctatcc atcatgatcg acggaacagt atatcccgat
     7141 gatgaccaac tagacggcga aagatttatg gaaatgatgg cgaaagcaaa aaatctgccc
     7201 aaaacaagcc aaccaccaat tggagaattc atctcgatgt atgatgagct aggagaagac
     7261 ggcagtgaag tgatttcgat ccatatgaca aaaggcttga gtggaacagt agaagcagca
     7321 agacaagcca gtaatctaac aaaaacgaaa gtaacggtta tcgacagtga taccactgac
     7381 caaggattat cttttcaggt aattcgagca gctgaattag caaaagaagg taaaacagta
     7441 gagaaaatcc ttgaagaagt caaaaaagta caagacaata caaaattata catcggaata
     7501 tccacactag ataacttggt aaaaggtgga agaatcagcc gaacaactgg tttgctgtct
     7561 agtgttttga acatacgcgt ggtgatgaat ttcgcccatt ctgaattaat ccctgtaact
     7621 aaggggcgag gaaagaaaac gttcgataaa tggtttaata atttgaaaaa tgaactgaaa
     7681 gaattgccaa atgtgcgaca aatcggtatt tcacatgcgg atgcaaaaga attggcgcaa
     7741 gaattcaaag agggtttgca agaattattc ccggacatgc atatccctgt actgcatacg
     7801 aatccggtta tcgctacgca tactggtaaa gacgcgtttg caatcatgta ttatgtagat
     7861 tagtcgaaaa gaggataagc cctcagcctg aaaggcttgc agcttattct ctttttcttt
     7921 ataaaattag gaaaataatt gttttctttt ttcataaaga ctaccttttt gataaaattt
     7981 ttgataacat tagaaccaat cgaatacaag aggtgaaaaa atgaaattaa cgaaacgaca
     8041 tttgatcctc gttttgttcc cattattaat tgcgatcatc atctatgcag gtttagctgc
     8101 ttctataccg aaagctggcc cactaatgaa accagtaact actgtgagag aagatccaaa
     8161 taaaaaagaa aaacttcact acatagctat cggagattcg ctgactgaag gagtaggaga
     8221 tcaaaccaag caaggaggat tcgttcccct tgtcgcaaat gatctaaaag accgttataa
     8281 tttgaccgcg gttgaaacag aaaactatgg tgtaaatggt gaacgaagtg accagatctt
     8341 aaagcgagta aaaacaaaag aagaaattcg aaacaatatc aaaacagctg attttatcac
     8401 actgactgtt ggcggtaatg atttgatgaa agttatccaa aataatttat tcgggttaac
     8461 ggtcaattcc tttaaaaagc cactgaaaaa ctatcaggaa aatgtcacag aactattgga
     8521 agaatttcga tccctcaata aaaccgcacc tatttacatt ttaggcatct acaatccatt
     8581 ttatttgaac tttcctgaga tcactgacat gcagacaatt gttaacaatt ggaacaaagg
     8641 aacagaaacg gtgacaaaaa aaatgccgaa ctgctacttt atcccaatca atgatttgct
     8701 ttatcaagga ttgaatcagg aagtaggtgt cacagaagaa ggaacagatc aaacaactag
     8761 cacgacagaa aatctaaatg gcatcaaaaa taatgtgctc tatgaagaag atcatttcca
     8821 tccaaataat ttaggttatc aattgatggc aaatgcagtg agagatcagc tgatcgagac
     8881 acaaaaccaa tggttagaga aggaggatga tcaataaaca tgaaaacaag atctgacgta
     8941 tcttcaaaca aaacaaagaa aacaaattac tggaaattcg ccttccttat ccttcttggg
     9001 gtagtacttg gttcgaccgt atttttaggc agccgtattt ttgctaatcg ggaaccagaa
     9061 ctcccagaaa taccagctct tacggaaaga caaggagatc ctgtcttaac gatcaattca
     9121 aataaagaaa aagtcaatca aatcatcagc tttttcctta gtgagtatca aaaagatagt
     9181 gatattgaat ataaatttta tttggaaaat gaagcactac tcaatggtac tttcgaagta
     9241 ctaggttttc ctatcgattt ctatttatat tttgatccgt atgtgatgga aaacggaaat
     9301 gtccaactaa aagctagaag tctttctatc ggaacattga gtttgccaat caaagatgtg
     9361 atgaatatga tcaagagaaa ctataaatta ccagattgga tcgaagtcaa tacagatgat
     9421 ctgactatca tgcttaggtt ggatcaattc cgaatgcaaa acgggatgta tattaaagca
     9481 gataaaatcg acttagtaaa tgatgacatt cggtttagtc tatatttgcc aaaagatacc
     9541 tctgatgctt caaacaactc aaataccaac aaaactagca ccagcagtac tgaaagcaag
     9601 acaaaagaaa ataaaaaatc atcggatgag tagtattaga gggggaatca taatggaacg
     9661 agcaatattt gcaggaggct gcttctggtg tatgatccag cctttcgaca cgcttcctgg
     9721 tatccataca gtgatgtcag gatatactgg agggcatgta ccagatccga cttatgaaca
     9781 agtaaaaagc caaacgactg gacatacaga agcagtggag attatctatg atccaacatt
     9841 gatttcatat gaacaattag tagaacttta ctggcagcaa actgatccaa ctgacgcttt
     9901 cggacaattc gaggatcgcg gtgacaatta tcgcccggtg atcttttatt tgacagaaga
     9961 acagcggatc attgctgaaa aaagtaagaa acagttagct gaaagtggac gtttcgaaca
    10021 gccaatcgtt acaaaaatcg aaccagccca accattttat ctagcagaag aagaccatca
    10081 agagtattat aaaaagcatc ctgaaaattt taaaagaaac catgcacgcc gggctgcctt
    10141 tatcgaagaa aattggagga acgaagggtg aatcgaagct tttttcatta tttaatgaca
    10201 ctgcgaggcg gtggcacaca tgatgcttta tctgttttcg ctacagaagc cggtaaagac
    10261 atccaatttc ctaagcacag ctctagctac gaagaaattt ctaattatct ggaactgaat
    10321 gtcgactatt tgccgtccat ggatatcttt gacgaagcat gggaaaaata tttagaaaat
    10381 aatcatgaca tataagagat agttttgtac tttatgatca aaaaagtttt ttgtccaata
    10441 gcctaattga aagaattatt tttatttcag ctatctagtc gcattttttt gatatttttc
    10501 ttataataaa gaaagaatga aaaaaagagg gatggattga tagagattga caaaaaaaca
    10561 acgctatatt aatcgcttct ggcttgtatt caaatactta cttgtttctg tattccttgc
    10621 gttcatgttg cggggatttt tattcatccc ggtaccagtg gaaggcaatt caatggaaaa
    10681 cgtgttgaag caaggtgata tggttgtgat ggaaaaattt tcggagatca gacgatttga
    10741 tattgttgtt tttcaattag cagatggcac gatttatatc aaacgagtaa tcggtcttcc
    10801 aggagaaaat gtcagctacc aaaatgatca attgaagatc aatgggaaag tagttaagga
    10861 accttatcta actaaaaacc tgaaatcaga tcatgcaaat gcatcctata cgacggactt
    10921 tacactacaa gagctaacgg gacaatccaa gcttccggaa gataattatt ttgtacttgg
    10981 agataatcga agagtttcaa aagatagtcg ttcgtttggc acgatcaata aaactgatat
    11041 tctaggtaaa gcaagatttg tctattatcc acttgatgaa ataaaatgga tccagtgaga
    11101 aacaataaag cgtgcagtct gttgagactg cacgctttat tgtttaatcc aaaatcagtt
    11161 tactacctta gtacgtaaaa tcaaacagct cgtttaaaca cttttctagc ctctaaagtt
    11221 ttgataaatg aataaacaac cattatttga atagggattt ggatcatatt cctgatgaat
    11281 ctacctagaa aaattacttt gaaatctgca ccaaacaaaa cagacatcag ccaagaattg
    11341 ataaaaaggc tgactgggat cataatcaat aattgggcag cgatgacgga aggcaggttt
    11401 atctttttat gatataaaaa taagccatta ataaatccac caaggaaagc cgtgataaga
    11461 aaagcagctt gaaacgttcc tgaaccaaat atcgtgaaat ttaaagtgtt cgacaacatt
    11521 tcaagcaaac cagcccagat cggtccatat agataaccca taagcgccat acttaaaaat
    11581 ccgaatgtga tttgaaaata agcaggacca atcgctatct tgtataagat gacatcaagg
    11641 gctaataaaa gagcagaggt cgacaacaat cttgcattca tttcgggcaa tctaaaaacg
    11701 tcttttgcaa tcaaagtgtt caaaatattc tctcctttgt cagttaataa taaaaggata
    11761 ccataaaatc accaatgtgt catgacacat tggtgattta catacactgg catctaacaa
    11821 ttaaaaaaag ggtgagaaga ctgcttttat aaactgtaaa aaagagattg tacgaataaa
    11881 aaaaatagag gcggagaata cttatcgtat catccgcctt tgtttttttg ttataaaaaa
    11941 cgtcagatta gttatctgct tacatcttca tccatacggg ttctaatgta tttccgtcta
    12001 gatcttggac ttctaatgaa aacatttctt cttcaggcat tcccatatct acttcgtgga
    12061 aagtaccacc attttctttt gcaatttgag caaatttttt tacttcatcc gcgttttcaa
    12121 cactaaacga aatcaaagca ccacttgttt ttttattatc agctatttct ttatctttta
    12181 taaatttttt ataaaaatca tgagagagaa gcattatcca aaaagtgtca tcccatacca
    12241 ttgaaccagc ttcttcagta gaaaattctt catttttttt aaaacccagc ttttcataaa
    12301 aagcaacgga acgattgaca tcagcaacag gaaaattaac aaaaaccata ctattcattt
    12361 gtttcgtctc ctttctgttt tgttcgtcta tcatctctta tttaggaaac gctgtcaaaa
    12421 atttagattc atatatagaa accaaaataa aaatttgcat catcaaaaaa gtgaagatgt
    12481 gatagaatga ggactggttc actttcactt aactaataga tggaatttct ttataataaa
    12541 aaagtgaatt tatattgaaa aagaaaaaaa caagtaaagc cggggaatat tccggtatga
    12601 aaggaatgga gagaacaatg accatccaat ggtttccggg acatatggca aaagcccgac
    12661 gagaagtttc tgaaaaaatg aaatatgtgg acatcgtatt tgaattggta gacgcacgat
    12721 tacctttatc ttctcgtaat ccaatgctag atcagatcat tcagcaaaaa cctcgccttg
    12781 ttttattgaa taaagcggat ctggcagatc cacaacagac aaagctgtgg caagaatatt
    12841 ttcgtaaaca aggccatctt gcattagcta tcacggcaca agaaagtaaa ggaattaaag
    12901 gtctagtacc tgcggctaaa gaagcattga aagaaaaacg ggcaagagat gctgctcggg
    12961 ggatcaagcc tcgcgctatt cgggcaatgg tcttaggtat tccaaatgtt gggaaatcta
    13021 cgttgatgaa tcgactagta ggcaagaaaa ttgcacagac aggaaataag cctggcgtaa
    13081 caaaaggtca gcagtggctt cgtttaggta atgagttgga attgttagac acaccaggga
    13141 ttttatggcc caaatttgaa gatgaagaaa tcggcaaaaa actagcgctg acaggcgcga
    13201 tcaaagacca gcttgtccat ttagatgatt tagcgattta cggattagac ttttttgccc
    13261 gatattattc aggaagaatc agcgatcgtt atggtttatc aatcgagcaa gaagaaaaat
    13321 tggcaccgga attactgatg gatattacag caaaacgtgg gtataaagaa gactatgatc
    13381 gtggcagcga attagtcgtg catgatattc gtcaaggaaa acttggacga tacacactgg
    13441 atcgttgcga agaaatggag caaaaaagtg atgactgaat ccatccaaag tatcaaagaa
    13501 cgattgaaaa gcatacagag aatagatgat cctacactag aatcattaag aaatgatcca
    13561 cgcaaaggcg tgcagcaagc actggcttct tttgaacgac gattgaaaaa ggaagcatta
    13621 attaaggaaa aatatgaaga aatgttttgc tttgaaaagc aagggtacgc acaaggtcac
    13681 caatggattg ccggcattga tgaagtagga agaggaccat tagctggacc tgtcgtggca
    13741 gctgcagtta tcttgccgaa aaatgagatc atattagggc tgaatgactc caaacaatta
    13801 tcagagaaaa agcgagaact tctttatcaa caagttcaag aaaaagccct ggcaatcggg
    13861 atcgggatcg ttgatcagca gacaatcgat gatgtgaata tttatgaagc aagtaaatta
    13921 gcgatgatcc aagcagtaga aaaattgtcc attgtaccaa gctatttgtt gatcgatgcg
    13981 atgaccttgg atttgcctat tcagcaggaa aaaataatca aaggggatgc acgatctgtg
    14041 tcgattgctg ctgcaagtat cattgctaaa gtatatcgag accaattgat gaaagaatat
    14101 gacgtgcttt atcctggata tggtttctcg cataatgcag gttatggaac aaaggaacac
    14161 ttagaaggac ttgaacgaca aggaattacg ccaatccatc gtaaaagttt tgcacctgta
    14221 aaaaaatatt tacaataaaa aacagccgct tttacgtatc ttattagaaa gagagacgaa
    14281 aggcggtttt atgatgtatc aaatagaaga aaatttattg aaacttgctt atacaaaagg
    14341 aatcagtagt caaggaaaat gggcacttgc taactggatg atgcagtatc cttataaaga
    14401 aatcggattt gaagacatca ttaagatagg taaaataacc aaacatcggg agctgtttcg
    14461 caaaagttgg caggaaattc acgctaactg gggcaaaatc caatcgaaac agtcgtttat
    14521 cacttgcttt gatcccaaat atccccctca gctgcttcac ttgacctatc cgccaattgt
    14581 actgttttat aatggtgatc tatctttact ttcttgtaac atgttaagcg ttgttggtgg
    14641 aagacacact tctgctttag ccaaaaaaac agtttattat ttactaaaac cagtgataga
    14701 agctggatat gtgatcgtca gtggctgcgc gaaagggata gatacatacg gtcaccaagc
    14761 agcaatctct catggtggac ggacaatcgc agtcattgga acagggatag agcaagtcta
    14821 tcctgctgaa aatcgtgact tgcaagaggt gattgcaaat gatcatcttc ttttgagtga
    14881 atatcctcct caatccggcc cgaaaaagca ttgttttcca atgagaaatc ggattatcgc
    14941 agctttgtct aaaggaacat gtgtctttga agccaagaaa aaaagcggct ctctgataac
    15001 agcacagcaa gcattggatc ttggaagaac agttttttct gttccaggaa atattttaac
    15061 agaacattct accggatgtc atcatttgat ccaagacggt gcagtttgca cggtttcggg
    15121 tgaagatatc ttggcagaat ttaaagaata acaccatact tttcatttgc ttttcttgac
    15181 aaacgttttc tgaatggata tgattagcta cgatttgtat gaatcctctt ttaatagtag
    15241 gaacacgaca atggaaagga gtccattcag taatggcgta taaatattta gtgattgtag
    15301 aatcacctgc aaaagccaag acaattgaga aatatcttgg acgaaactat aaagtaatgg
    15361 ccagtgttgg ccatataaga gatttaccga aaagtaaaat gggtatcgat tttgaaaaca
    15421 attatgaacc ccattatatt tctatacgcg gaaaaggcga tgtcatcaaa agcctgaaag
    15481 ccgcagcaaa aaaagctcaa aaagtttact tggcaagtga cccggataga gaaggagaag
    15541 cgattgcttg gcatttagcg tacctacttg ggttggatct gaaagaaaaa aatcgggtgg
    15601 tcttcaatga aatcacaaaa gacgcagtca aagcagcttt taaggaacca agaacgatcg
    15661 atgtagattt agtagatgca cagcaagctc gtcgtacctt agacagaatc gttggttatt
    15721 cgatcagtcc tattctctgg cgtaaggtca agaaagggtt aagtgcagga cgtgtccaat
    15781 ctgtcgcttt aaaaattatt attgaccgtg aaaaagagat ccgagaattt gttccagaag
    15841 aatattggag catcgacggt aattttaaaa aagctcgcaa gaaattcaaa gcaaatttct
    15901 ggggaatcga cggtaagaaa aagaaattac cagatgcaca aagtgtaaaa gaagtcactg
    15961 ctagaattga tggaacagac ttcgaagtta aaaaagtaga aaagaaagag cgcaaaagaa
    16021 accctgcgaa tcctttcact acaagtagcc tgcaacaaga agctgcccgt aagttaaact
    16081 ttcgaacaag aaaaaccatg atggtagcac agcaattata tgaaggaatc actttgggta
    16141 aacaaggaac agtcggcttg attacataca tgcgtactga ttctacccgg attgccgata
    16201 cagcaaaagc agaggccgca gcatttattg aagatacgta tggaaaagaa ttttctagac
    16261 atgatcaccg aaagatcaaa aatgctcaag gcgcacaaga tgcccatgaa gctgtgcgtc
    16321 caacgagtgt gcttcgtaca ccagatgaac tcaaacaata tttagataaa gatcaattga
    16381 aactttatac attgatctgg tcacgttttg tggctagtca gatgacacca gctattttag
    16441 atacaatgaa agtgacatta gaacaaaatg gtgtcatctt cattgcgaac ggttcaaaaa
    16501 tcaaattcaa aggattcatg caagtgtatg tggaaggtag agatgacggc aaagaagaga
    16561 aagaaaatat tcttccagaa cttgaagaag gcgatgtagt ccaatctgtt gatattgaac
    16621 caaaacaaca ttttactcaa ccaccagcac gttttagtga ggctacgttg attcgtgcac
    16681 tagaagaaaa cggggtaggg cgaccttcca cttatgcacc aacacttgat acgatccaaa
    16741 gacgctacta tgtaaaattg acacaaaaaa gatttgagcc gactgaacta ggagaaattg
    16801 tcaattcatt aatctgtgaa ttcttccctc aaatcgtcga tatccatttc acagcagaaa
    16861 tggaaggaga tttggataaa attgaagaag gcacggaagc gtgggtcaaa gtggttgatc
    16921 gtttctataa accgtttgaa aaagaactga cgaatgcgga agaaaaaatc gaaaaaattc
    16981 aaatcaaaga tgaaccagca ggattcgatt gtgatgtctg tggacatccg atggtgataa
    17041 aattagggaa atatgggaag ttttatgctt gcagtaattt tcctgattgt cggaatacga
    17101 aaccaatcgt aaaagaaatc ggtgtgactt gcccagtatg tcatgaagga caagtcattg
    17161 aacgaaaatc taagaaaaac agactattct atggttgttc ccgtttccca gaatgtgaat
    17221 tcacttcttg ggataaacca gtgggcagaa attgtccaaa atgtgacgga tatctggtcg
    17281 agaagaaagt caaaggcggt aaacaagtag tctgtgtaaa tggagattac gaagaagaca
    17341 tccaaaaata atagaaaaga ggttctttca ttgaaaatgg taaatgttgt tggagcaggg
    17401 cttgccggaa gtgaagctgc ttggcagatc gcacaagcag gagtccctgt ccgtttatat
    17461 gaaatgcgtc cagtcaaatc gactgaggcg catcacacaa gtaattttgc tgaactagta
    17521 tgttccaatt cattaagagg aaacagtttg gctaatgcag tagggttatt gaaagaagaa
    17581 atgcgccgat tgaattcggt agtaatcact tcggcagatg aaacagcagt gccagctggt
    17641 ggtgccttag ctgttgaccg cgattctttt tctgaaacaa ttacccgaaa agtcaaagag
    17701 catccattag taacagtgat aaacgaagaa attactgaaa taccggaagg aatcaccgta
    17761 gtagcaacag gacctttgac ttctcatcca ttagcagaaa gcatcaaagc attcaatggt
    17821 tcagatggtt tttatttcta tgatgcagct gcaccaatcg tagacaaagc tacgatcgat
    17881 atggataaag tatatttgaa gtcccgctat gacaaaggag aagcagctta cttgaactgt
    17941 ccgatgacag aagaggaatt caaagctttc catgaggcac taatcaatgc cgaagtggca
    18001 gaacttaaat cttttgagaa agaaaaatat tttgaaggat gtatgccaat tgaagtaatg
    18061 gcacagcgag gattcaaaac gatgcttttc ggacctatga aaccagttgg attggaagat
    18121 cctaaaactg gcaaacgtcc ttatgctgtt atccagctgc gtcaagacaa tgctgcagct
    18181 tctttataca acatcgtggg ctttcagact cacttgaaat ggggcgagca aaaacgtgtt
    18241 ttccggatga ttcctgggtt agaaaatgct gaattcgttc gctatggtgt gatgcaccga
    18301 aacagcttta tgaattcgcc agaattgctg aaaccgactt accaatcaac gaaacgcgaa
    18361 gatctgttct ttgcaggtca gatgactggt gtagaaggct atgttgaaag tgcagcgagc
    18421 ggactacttg ctggaatcaa cgccgcacgc ttagcaaaag aaatggaacc agtcgagttt
    18481 ccacaagaaa cagctattgg aagtatggct tactacatta ctcatgcgga aggaaaacat
    18541 ttccaaccga tgaatgccaa ctatggtctg tttcctgaac tgccagagcg cattcgagac
    18601 aaaaaagcaa gatatgaagc aattgcacaa cgagctttaa aagctaatga ggcaataaaa
    18661 acagaattag aaaaaactgc aatcccaaat gagtaaggaa actccatgaa gagggtgaga
    18721 cggatagtct cgctcttttt
//
LOCUS       DAJSBL010000053        18136 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.055, whole genome shotgun sequence.
ACCESSION   DAJSBL010000053 DAJSBL010000000
VERSION     DAJSBL010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18136
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.055"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(27..725)
                     /locus_tag="ONT85_002144"
     CDS             complement(27..725)
                     /locus_tag="ONT85_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HCR2379104.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(718..2070)
                     /locus_tag="ONT85_002145"
     CDS             complement(718..2070)
                     /locus_tag="ONT85_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HCR2379105.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(2293..3693)
                     /locus_tag="ONT85_002146"
     CDS             complement(2293..3693)
                     /locus_tag="ONT85_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HCR2379106.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            3854..4591
                     /locus_tag="ONT85_002147"
     CDS             3854..4591
                     /locus_tag="ONT85_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HCR2379107.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            4716..5195
                     /locus_tag="ONT85_002148"
     CDS             4716..5195
                     /locus_tag="ONT85_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HCR2379108.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            5192..5959
                     /locus_tag="ONT85_002149"
     CDS             5192..5959
                     /locus_tag="ONT85_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2379109.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            6189..6758
                     /gene="lepB"
                     /locus_tag="ONT85_002150"
     CDS             6189..6758
                     /gene="lepB"
                     /locus_tag="ONT85_002150"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HCR2379110.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(6862..7464)
                     /locus_tag="ONT85_002151"
     CDS             complement(6862..7464)
                     /locus_tag="ONT85_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HCR2379111.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            7570..8039
                     /locus_tag="ONT85_002152"
                     /pseudo
     CDS             7570..8039
                     /locus_tag="ONT85_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            8145..8729
                     /locus_tag="ONT85_002153"
     CDS             8145..8729
                     /locus_tag="ONT85_002153"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HCR2379112.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            8732..9805
                     /gene="prfA"
                     /locus_tag="ONT85_002154"
     CDS             8732..9805
                     /gene="prfA"
                     /locus_tag="ONT85_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HCR2379113.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            9798..10637
                     /gene="prmC"
                     /locus_tag="ONT85_002155"
     CDS             9798..10637
                     /gene="prmC"
                     /locus_tag="ONT85_002155"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HCR2379114.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            10630..10809
                     /locus_tag="ONT85_002156"
     CDS             10630..10809
                     /locus_tag="ONT85_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2379115.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            10831..11856
                     /locus_tag="ONT85_002157"
     CDS             10831..11856
                     /locus_tag="ONT85_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HCR2379116.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            12031..13275
                     /locus_tag="ONT85_002158"
     CDS             12031..13275
                     /locus_tag="ONT85_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HCR2379117.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            13428..14375
                     /locus_tag="ONT85_002159"
     CDS             13428..14375
                     /locus_tag="ONT85_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR2379118.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            14509..15138
                     /gene="upp"
                     /locus_tag="ONT85_002160"
     CDS             14509..15138
                     /gene="upp"
                     /locus_tag="ONT85_002160"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HCR2379119.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(15277..15519)
                     /locus_tag="ONT85_002161"
     CDS             complement(15277..15519)
                     /locus_tag="ONT85_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2379120.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            15640..15712
                     /locus_tag="ONT85_002162"
     tRNA            15640..15712
                     /locus_tag="ONT85_002162"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15673..15675,aa:Thr,seq:cgt)
     gene            complement(15831..16469)
                     /locus_tag="ONT85_002163"
     CDS             complement(15831..16469)
                     /locus_tag="ONT85_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HCR2379121.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
     gene            complement(16592..17224)
                     /locus_tag="ONT85_002164"
     CDS             complement(16592..17224)
                     /locus_tag="ONT85_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HCR2379122.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
     gene            17575..17874
                     /locus_tag="ONT85_002165"
     CDS             17575..17874
                     /locus_tag="ONT85_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HCR2379123.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
BASE COUNT         6220 a         3162 c         3573 g         5181 t
ORIGIN      
        1 ttgtcacatt cccctttttc gaataactag gctgtttgtc cttcaagata gggcagatcc
       61 aattctttac cgtaacgttg ttctaaagct aggcggatca aacgagctgc caggttctca
      121 tttcttgcaa ggataggtcc atggaaataa gaaccgaaga catttcggta aatgacacct
      181 tcagattggt cttcaccatt atttccttga ccttgtacca ctttccccaa aggtcgttca
      241 ccttcaccta agaaagtccg accattgtgg ttttcaaatc cataataagt ttcattgaat
      301 tcttcgttat ggataacgat atcaccgata aaccggttat tttcctgact taacgtgtag
      361 tgatccagtg cactgattcc ttggatcttt tctccattag ctcccatgta gtagtgacct
      421 agcaattggt agccaccaca aatggctaac atgacgccat cattttcgat atagttgatc
      481 aaagaatctt tctttttctg gatgtctttt gaaatgatta attgctcaaa gtcttgacca
      541 ccgccaaaga aaaccaagtc atatttatca gcatcaaaag gttcatgtat actaacgatc
      601 tccgtatggc aagaaacccc cattttttct gctatatatt tcaacatcaa aaggtttccg
      661 ttatcaccat atgtgttcat caaatttcca taaaggtggg cgatattgag ttcataatta
      721 gccacggttc attcctcctt tgataaatcc ttgagcagct agttctttac gaagctgcaa
      781 tacagcagtg taagtagcta aaatatagac atgctcagtt ggcaattctt tgattttttc
      841 gattacttct ggtaattctt ttgtttctgt caatttattt tctggaatcc cggcaacttt
      901 tagacgtaaa gtcatatctg tatgacggtc tcctccagaa ataacagctg ggatatccat
      961 atcggcaaag ttttcaaaat taccatccca aatccagctg acatcgattc cgtctgcgta
     1021 attggcattt aataaagcca ctaaagaaaa tggataagga gctagaccaa tcatatcgat
     1081 tacttgattg atacctacag gatttttgac aagtactaat gtacattttt tcccattgat
     1141 attgatgact tcttgtcggc cgaatacttt ctcgtcatat cctaaacctt gtttgatttt
     1201 atcaggatcg acattgaagt actctgccac tgcagtagct gctaaagcat tgtagacatt
     1261 atacataccg ccaacttcga ttccgtattc atgaccatcg atcacgaaat ctgctgacac
     1321 attgtccatc cgtaccatat cagttaatcg ataatccaat tcaggacgtt tgaagtcaca
     1381 atttggacag taatatttcc ctaagtttgc ataagtgatc attttataat gcaagatgtg
     1441 atgacaattt ggacataaga caccgtctgt attgtaatga gccatttgtt cacgatcttc
     1501 ttcatggtca aagccataat attttctagg attgactgtt tcgatagaat tgaagattgg
     1561 tgaatcaccg ttactgataa tcgttgcatt tggtgacttt gcggcaccgt ctacaatcat
     1621 tttataggtc gtatagattt ctccataccg atccatttga tcacggaaaa tatttgtaaa
     1681 taagaacagt tctggtttca tatattctgt gactttgctc aaacttgctt catctatttc
     1741 taggacagca aactttttct gtcctttttt tggttttgcc tgcaagaaag tcgatacaat
     1801 tccctgtacc atattcgcac cagtagggtt ggttagaact tcatcgaatt gctggcgcaa
     1861 aatattgact gttaaagcgg tagtcaatgt tttaccgttt gttccggtga caacgacgac
     1921 ttggtagtct tttgccaaac tatctagtat atgtggatcg actttcagcg ctagtttacc
     1981 tggtaaactt gatccgcctt taaaaaaagt ttttaatgcc cattgcgacg tttttccaac
     2041 tgcaatcgca aaatgactgc gtattcccat tatgtaacct ccaaaaacga tttgctgacg
     2101 aataaattca actaatcata ccatatgttg aaaaaaaggt taaattgctt ttcttattct
     2161 taagaaaagg ttacggctga aagtgcatga aatcggacaa aatcgcatga tttaacaaaa
     2221 aaacagcttg aaacaaaatg attgtttcaa gctgctgtga ttttattatt ccctcttacc
     2281 tatattccca ttttaaccga taataatttt ctcgcttgga tattcatagc ctagatctcg
     2341 tgtttctttt ggcacgaata acatcaatgt atatagcatt ccgatccgcc caataaacat
     2401 cagtgtagca atcgtgattt tgcccacaac agacaagtct ccggtaatcc ctaaagataa
     2461 accagttgtt ccaaacgcag aagttacttc gatgatgatc gagatcagcg tttgttcttc
     2521 tgtagcagat aaaaaaacga tacaaaagaa acacatacca agagaaagca taaagacaac
     2581 gactgatttt cgcacatcat cttgatcgat tctccgtcca aaaatattga tgttatcttc
     2641 acttttgagg aaggaatata gatacaaacc gatgattgct acagtagttg ttcgaatccc
     2701 gccgcctaca gaactaggac tgcatccgat aaacatcagt aaagaaaaaa tgatcagtgt
     2761 tgtgatttga aaatcaccca aatcgtgaat ctgcagacca gcattacgag tcgtaatcga
     2821 ataaaacatc gaattgatcc atttgacact catattggaa tcttggaata aatggtcctt
     2881 ttccaataga taaatcaaga cagttcccga aacaaacaag ataacaaaag ctaacacagc
     2941 taatttggta aacaaggaaa aacgaaacgg aagcttcgca ttggaacgtt tgtataatag
     3001 ccattcccgg cactccatca atacaggaaa accaattcct ccgacaaaaa tcaaaaacat
     3061 gatcaggatc aagaaaaaat agtcatgtgc aaagggcttg atggaatccc cagtgacgtc
     3121 aaacccagag ttcgtgacag ccgatatcgc ctgataaaaa ccgtaaaaaa cggcatcacg
     3181 ccaacgatca aaatagcctc tgtaataaaa ataaatagaa aagaatgtcc caaaaagtaa
     3241 ttgaaaccag attaaaatag caaaagtgat ccgaatcagt cggacgatac cagacaaacg
     3301 cggttgattc atatccgtca tgatcagctg tcgctgtttc agtgtgatcc gtcgtttaga
     3361 aaaaatgaca aaagcagtag aaatcatcat gatccccaaa ccaccaactt gaaataatat
     3421 ttccagcagt ataataccgt tatcattgaa cacagaattg atatcaaaag tcgatagtcc
     3481 agtcacactc actgtactga ttgccatgaa cagcatgtcc acaaaagaaa catggctatc
     3541 tggttcgcga aaaatcggca tataaaatag aacgagtgat aagaccgtca tcaatatata
     3601 ataaaaaacg atgatttgga tcgatgagaa atgattggca gcaaacttct gtcctcgccg
     3661 ctgcagcctt cgaaagaata aatcaacatg cacagtacta tcctcctgac aatagtcgtt
     3721 tctaccagta tacaagggga attaggaata ctcaaagaaa ttatcaaaca gataacaata
     3781 cagctttcga ttaaaataga aatggcttat aataaggaaa atcctagaaa aaagaaggag
     3841 gaggaataac aaattgatta atgctcaaca aatcgtagaa aaaatgaaaa atcaaaaaat
     3901 aaattatgat acagttctga aaaaaatgat catgcaatgg gagaaagaag aagtaaagcc
     3961 taagatatta atacatacat gctgtgctcc ttgcagtacg cacacattgg aattcatgtc
     4021 tcaatatgca gagatcacgt tatttttttc aaattcgaat attcatccta aaagtgagta
     4081 tttgcgaaga ttacatgaac aaaaacgatt tgtagaccaa tttaatgaga aaactgacca
     4141 tcatgttgcg ctaatcgttg atgaataccg tccaaatgaa ttcaccaaaa tggttttaac
     4201 aaaccatctt gaaaacgaaa aagaaggtgg tgctcgctgc agtgcatgct tcaatatgcg
     4261 attagatctt gttgcgcaaa aagcgcaaga actaggatac gattactttg gaagtgccct
     4321 gaccatctcg ccaaagaaaa atgcagcttt gatcaatcag atcggaatgg atatccaaaa
     4381 gatttacggg acacaatact tgccaagtga tttcaagaaa aacaaaggct atgaacgctc
     4441 tttgcaaatg tgcaaagagt atgatattta tcgccaatgt tattgtggtt gcgtgttcgc
     4501 tgccaagcag caaggatgcg atctgaaaga aatcaatcgg gaagcaaaag cttacttaaa
     4561 aacatcaacc gtagtttttg aggaaaatta aagaaatctt tcggaaaaac gtaagaaaaa
     4621 aataaagaat ttatggtaga aataaggtag actgtataat ttgtccgtag taaagtaagg
     4681 aaacaagaat aaatttcaat gaaggaagag ataagatgcg aaaagtagca cctattttga
     4741 agccaatcgc tgtattaaca atctcgacga ttgtcgcgac tactacaggt attgtgaata
     4801 gttccagcta ccgtgcacca agttcaagtt ctagtactgt agaagaaaca acaccaacta
     4861 cttcagtctc gacatctgaa ccaacaacaa cggaaagttt gaatgaaacc actacgacaa
     4921 gtacatggga cgatacaaca actagttcgt cgtatgatga gacatataca actgacagct
     4981 caagcgaaac aagtaatacg gtaccagatg gcactccatc ggattcaacc aatccaagta
     5041 caagtccgga tacaagtgct agctccgaaa cgataccaag cagtacgact gtagatgata
     5101 acacgcaaaa tgatgatgca gcacgtacag cacagcaaaa ccaagattca ggaaataccc
     5161 aaacgtataa ccagcaagca ggtgattctc aatgacattt ttactagacc ttatcaacgg
     5221 ggtgcataaa ttccttggct atttagacat cagtcctaaa tatcttaacc gtggatatac
     5281 gattttaagt gtgattccca cgctttattt attgagaatt gtttatggac tttggcaaaa
     5341 ccaaaactat cttcaatttt tcttatatgg acttgcattt ctagtgcttg tttattttac
     5401 tgttttaaat gtattttatt actttttaga taaaaacacg aaagccgatg tcactcagct
     5461 ttttgtgaag tatttacctg atgaagcgtt caacatccag acagaagaat cgatagataa
     5521 tggaaattcg atcgataaag tgaatacaca agaggtcatg gtttcttatg acgaagatta
     5581 tcaattgaaa ttagccgaga atatgcggta tttgattgga aatggtgaga tcaaaacaaa
     5641 tgaactcggt cgcatggatg gatttctggt cgatagaaat acattgtatc cttattacta
     5701 tgtcaagaaa acaggagaga aagagtatgt tctaaatatt ggacgaagct actcggacat
     5761 ggaaaaagta gggaccattc gacttgactc aacagaagac acattggaac ctgtcggatt
     5821 gttcatcgtc ggcggtgatt ttgtcaaaga cgggttacgc tatcatgagc cttatcgtct
     5881 gaaattgatt gcaaaaaagg cacagtcgca ggaagaacca gtggtttata gtcgtagcca
     5941 aagaagaaga cattcataat gtgatgaaac gccgtgtatc agtggttgcg ataaaaacgt
     6001 ttattcggaa taagggggat gtgacacgtc ttttgtcacg ttcctttttt atataacgag
     6061 aaagataaga cgaacacatc acttattctt tcctttcatt tataaacatt gtagaataaa
     6121 gaaagtacaa cgaagtattg tgaaaaaacg taataaataa cgaggaaaat acaagtagga
     6181 ggtaagctgt gaaagaattt ttgaaaaatt gggggatttt gatccttgta ttagcggcta
     6241 ttttattagc tagagtgtac gtgtttacac cagttactgt aaacgggcat tcgatggacc
     6301 ctacattaag tgatggacaa cgattgattt catctaagat ctcaaactat gaacgaatgg
     6361 atattattac tacaaaggaa ccgggggatg aagaacggat gattgttaaa cggatcatcg
     6421 gtatgcctgg agatacagtc aagatggaaa atgaccagtt gaccattaat ggcaaaaagt
     6481 atgatgaacc ttatctggat gagttcaaaa aagaattttc agaagataag ttacaaggag
     6541 aatatgctta tagctcagga ttccaagcac aagcagaaag ttcatccacc tttacgagtg
     6601 actttgaata tactgtgcca aaaggaaagt atcttgttct aggcgataac cgcttgattt
     6661 caaaagacag ccgaatgttt gggttagtcg acaaagatat gatccaagga aaagttgttt
     6721 tccgttattg gcctctttca gaaattaaaa tcatgtgatg aaaaaataaa aagaagctgt
     6781 gacatgtttt ttgtcacagc ttctttttcc gaaaattcct tcttatttct tggagttgct
     6841 cacttttttt gcaatctcat tttaagcttt ttggctgatg gataagaaaa gcgaagcgtt
     6901 tttgttcata aatagctttt ccccacgatg gaaggtatga ctagtagcag agacaaagcc
     6961 cgttttttcc aatcgattat tcaattcttc ttggctaaag ccattatgga ctttcggatg
     7021 actaatttgt tcattcttgt caaagtctac gatgatcaat tgaccgccag gagctaaaat
     7081 ttcataaagt tttgtaagaa tattctccgt atcaggaatg tgtaaaagta caagtgatag
     7141 aaggatgaga ttagcttgga cagcaggaaa ttcgacagaa gcatcagcgt ggatcgttcg
     7201 gacatttttc agatctgctg cttggatctt ttcttctgcc attttcagca tagtttctga
     7261 agcatcggca atgatcagtt ctttgaatct ttccgcaagc ggtaaactaa caagcccagt
     7321 tccgcctccg taatccagca ggactttttg gtggctgtcg cgcggtagat aagtagttag
     7381 ttcttcgcga ataatcttgg ctaatgcttg acggtcaggg gaatcataat ggttagcgat
     7441 ggaatcaaaa atattttttg tcatcaaata gctccttacg taaaactttg aaattttaac
     7501 ttattatagc acgtgaaaag aaagaaagca tgctatactc aattcaaaaa gggagaatct
     7561 agaaagaata tgccgatatc gtttgtaaaa gatagagagg aaaaaggaaa gtgtgtccga
     7621 gaaatattac tagacttgcc tgagtggttt gggcttcctg aatcaacgga aaaatatatc
     7681 gaggaatcaa gtaaactacc tttatggtgt gaaaaaagaa aagaagaata tttaggcttt
     7741 atcactcttt cacagacaag tgaagatact gccgaaatct attctattgt atgggaataa
     7801 aaaaagacta tcatcaccaa ggattaggaa caaaactttt taaagaagca gaaggttacg
     7861 cagcaaaaca ttacaaatat ttgcaagtga agactgtaga cgaggggcac tactctatat
     7921 atgatcaaac tatttgtttc tacgaatcat taggattttc aaggttggaa gtttttccaa
     7981 atttatggga tgaatggaac ccatgtttag ttcttgtcaa aaaattggaa caaaagtgag
     8041 caatagattt aatactaatc tgtgcttgat gaaaattctc actgtaaaaa atgctgaaaa
     8101 tcaagtataa taccatagga acaaactgga aggtgaaaaa gagcatggca caactatttt
     8161 ttaagtatgg cgcaatgaat agcggcaaga cgatcgagat cctaaaagtc gcacataatt
     8221 atgaagaaca aaataagcca gttgtcttaa tgacaagcgg tattgataca agagatggtt
     8281 taggcgttgt ttcaagccga atcggactga aacgtaatgc gatgccgata tttgaagaaa
     8341 cagacgtatt tgacgtaatt cagaaaatgg aaagcaaacc ctattgtgtg ctgatcgatg
     8401 aagcgcaatt cttaaaaaag aaacatgtca ttgcttttac gagaattgtg gacgaattgg
     8461 atattccggt tatggcattt ggtttgaaaa atgattttcg aaacgagtta tttgaaggat
     8521 caaaatattt attgctttat gcagacaaaa tcgaagaaat gaaaacaatc tgttggttct
     8581 gccacaaaaa agcaatcatg aatttacatt atattgatgg aaaccctgtt tatgaaggcg
     8641 atcaagtgca aattggcgga aatgaagctt attaccccgt ttgtcgaaaa cattactttc
     8701 acccatcaat gattacagaa ggagaataag catgtacgat caattacaag caattgaaga
     8761 ccgttatgaa gagcttggag aacttttaag tgacccagaa gtgatttctg atacaaagcg
     8821 ttttatgcag ctttcaaaag aagaagcaaa cacgcgcgaa acagtcgaag tttatcgtcg
     8881 ttataaagaa gtagtacaag gaatcaccga tacagaagaa ctgcttggtg aaaaactgga
     8941 tgatgaaatg gctgaactag caaaagaaga attatctgaa ttaaaaaaag aaaaagaagt
     9001 cttagaagaa aaaatcaaga tcttgctttt accaaaagat ccaaatgacg ataaaaatat
     9061 catcatggaa atccgtggag cagcaggcgg agacgaagca gcattattcg ctggcgatct
     9121 tttcaatatg taccaaaagt atgctgaagc acaaggctgg aaaacagaag tgatggaagc
     9181 taacgttacc ggaatcggtg gatataaaga agtgatcatg atgatcaccg gagacaacgt
     9241 atattcgaaa ttaaaatatg aaagtggtgc gcaccgcgtc cagcgtgttc cttcaactga
     9301 atcgcaaggg cgtatccata cttccaccgc aacagttgtc gtgatgccag aagctgaaga
     9361 agtagagatc gatattgaag acaaagatat ccgtacagat atctatcatg cttcaggtgc
     9421 tggcggacag cacgtcaata aaacagcatc tgctgtacgt ttgactcact taccaacagg
     9481 gatcgttgtt gctatgcaag atgaacgttc tcaaatcaag aaccgtgaaa aagcaatgaa
     9541 aatcttgcgt gctcgtgtgt acgataagat ccagcaagaa gcacaaagcg agtatgatgc
     9601 caatcgaaaa tcagcagtcg gaactgggga tcgttcagaa cggatccgga cgtataattt
     9661 cccgcaaaat cgtgtgactg atcatcgtat cggattaact atccaaaaat tagatcagat
     9721 tttagcaggg aaattagatg agatcgttga tgcattgatc atgtatgatc aaacatcaaa
     9781 actggaagct atgcaaaatg ggtaaaatga cctatcgaga agtcctgtca cgggcttctt
     9841 cttttttaga ggatcatggg aaagaagcat atagtatcca atttttattt ttggaacgaa
     9901 aacaatggaa aaaacttgat tggctccttc atatgaacga agagatttca gaagaagaac
     9961 agcggttgat cgaaaccgat ctgcgacttc tgcttgcaga tcatccacct cagtatttgc
    10021 taggatatgc agatttttat gatcatcggt taaaggtaac tgaagcaaca ctgatccctc
    10081 gaccagaaac agaggagtta gtcgaatggt gtttagacga gacgcctggt gtaccgttag
    10141 aagtgataga tattgggaca gggactggag caattgctat tagtttgaaa gcagcaagaa
    10201 aaaattggca tatatctgct gtggatcttt cagaagaagc attagaagta gcgaaagaaa
    10261 atgctcagaa agaaggaacg aaaatcagtt tttatcatgg agatacatta ggaccagtaa
    10321 tggatcagca atttgatgtg attatttcta atccgccgta catcagtcgg aatgaatggg
    10381 aactgatgga tgaaagtgtc cggtcattcg aacctaaaat ggcccttttt gctgagaacg
    10441 atggattagc tatttatgaa aaaatagcag ttgaagcacc aagcgtacta aaatcaaatg
    10501 gaaagatttt tttagaaatc ggatttcgcc aaggtgaagc agtaaaaaaa atatttcagc
    10561 aagcatttcc agataaaaaa gtggcgataa aaaaggatct ttttggcaat gaacgaatga
    10621 tccaagttta tggttgaagt tgtggatgaa aaatttttta tttgtttttt tattataaaa
    10681 aaacacttat ttgacaagga tgtaaacgac tttttggttt atcaacaagt ttatccacta
    10741 ttgacaaata gattatccac aaacagtgga aaacttgagg ataaactcag acaaaataag
    10801 aatgtatgaa aaaggggttg tggataaact gtggaaacga aacgatatga tgaaacagaa
    10861 ttgcaagagg cagccgaagc actgaaagaa ggagaacttg ttgcttttcc taccgaaaca
    10921 gtatacggat tgggcgccaa cgctctttta ccaaacgctg tcaagaaagt gttttctgta
    10981 aaagggagac cgcaagataa tccattaatc gtccatgtcg cctcttttga acaagtaaaa
    11041 gagtatgtgg acaactttca tccggcagca gagaagattg tgaagaactt ctggcctgga
    11101 ccattaactt tgatatttaa gatcaaaaaa gacactttgc cctcagttgt gacgggagga
    11161 ttatccacag ctgcatttcg tataccggat aacaaaaaaa cactagaagt aatcgaactt
    11221 tccggtgttc cgcttgtcgg tccaagtgca aatacgtcgg gaaaaccaag tccaacgact
    11281 gcagatcatg tatatcatga tctacaagga aaaattaccg ggattattga tgatggagct
    11341 acgcgaattg gtgtagaatc cactgttctt gatttaagtg atccaacagc catgcctatg
    11401 attttacgac caggtgcagt aacgaaagaa caaatcgaag cagtcatcga atctcctgtt
    11461 gctattgatc agcatcttgt aaaagaaaat gaaacaccaa aagcccctgg gatgaaatac
    11521 aaacactact cacccgatac acgtgttctg atggtcagag atggcgattg gtcaactgca
    11581 gttcaatggg caaaaaataa gaagatccgt gcaggtgtca tagcaagccc cgaaattgcg
    11641 gatcaagtgc ggacagatac tgcagctgtt tatatgtata acgacaactc agtagaagcg
    11701 gcggctaaag gattgtttgc aggtctaaga ggattagatg aaccaacact tggattagat
    11761 ttgatttttg tacaagtgta tccagaaact ggattgggga atgcttacat gaatcgcttg
    11821 aaaaaagcag caggacaaaa ttattttgaa aaatgatcaa ttggttattg tcgcattctt
    11881 ttagtatgat acaatgctat caagtaaatc ttctatcagt gaacgaaaaa ctaagcgtaa
    11941 ctaatcgtaa tagaagagat acgaatattt ggcaatttgt ttttgatggg aaaaataaaa
    12001 ttgtaggaaa ataaaaagga gctgttgttt gtggtagatt acaaaacgtt tgaccctgat
    12061 ctttgggctg cgatcgccaa agaagaagaa agacaagaac ataatttgga gctgattgcc
    12121 tctgaaaact ttgtctccga agcagtgatg gctgcacaag gaagtatctt gacaaataaa
    12181 tacgcagaag gttatccagg gcatcgctat tatggtgggt gtgaatttgt cgatatcgtt
    12241 gaaaatttag cgattgaccg tgcaaaagaa ttattcggag cgaaatttgc gaacgtccag
    12301 cctcactccg gctctcaagc caacacggct gcttatctcg ctttggtaga accaggggac
    12361 acgatcttag gaatggatct ttccgcaggc ggacatctaa cacatggatc accagtcaac
    12421 ttcagtggca aaacctatca ctttgttgct tacggtgttg atccgacaac agaagttatt
    12481 gattataatg tggtacgtat tttagctaga aaacatcaac caaaattgat cgtagcaggt
    12541 gccagtgctt acggacgaac gatcgacttt gcgaaattca gagaaatcgc agatgaagta
    12601 ggcgcaaaac tgatggtaga tatggcacat atcgcaggat tagtagcagc aggacttcat
    12661 cctaatccag ttccttatgc agatatcaca acgactacca ctcataaaac attgcgtggc
    12721 cctcgaggcg gcatgatttt gacaaacgat gaagcattag caaaaaaaat aaatagtgcg
    12781 gtatttcctg gtattcaagg cggccctcta gagcatgtga ttgcgggtaa agcagtagca
    12841 ttcaaagaag cattagatcc tgcatttaaa gaatatagtg aacaaatcat cgccaatgca
    12901 aaagcaatgg tcaaagtttt caatcaagca attggaacca gagtcatttc aggggcgaca
    12961 gataatcatc tgatgttgat cgatgttcgc gagttaggta taaatggaaa agaagcagag
    13021 agcattttgg acagtgtgaa tatcactgta aataaaaact cgattccttt tgaaacacta
    13081 agtccattca aaacaagcgg tattcgaatc ggcacgccag caatcactac tcgcgggttc
    13141 aaagaagaag atgctgtaaa agtggctgaa ctagttgtca aagcattaca agcaaaagat
    13201 gataacgctc aactagacga agtcaaaaca ggtgtacgcg aattaactga aaaatttccg
    13261 ttgcataaaa aataaaaatt taaattccat tagtaaacag aaaagttcaa tgttgggctt
    13321 ttctgtttat tttttgggaa aacggattct ttgacaatat cggtgatgct ctctagtata
    13381 tgaggtgtaa ttttagtctt cacactaaat ctcagggagg aaaagtcatg actgatcgta
    13441 tctcgttaga aaaggaagca gtagcattca gtgatgcaaa tgcgcctcat ccgcgtatct
    13501 atgaactagc accggaagaa ggaagagttt tactagaaga agtacaagaa tcacctgtga
    13561 acaaatattc cgtcgatatt gaagacttga tggtggatac tggcgaatgg ggaaaactca
    13621 atattcgtct cgtacgacca gaagggaata tggataaatt accagttatt ttttatattc
    13681 atggagcagg ctgggttttt ggcagtgctc aaacccatga taaattgatc agagaacttg
    13741 cggttcgtac gaattccgtc gttgtctttc cagaatatag tcgatcacca gaagcaaaat
    13801 atccaacagc aattgaacaa aattatgctg tgttgcaaca gctgaataaa ttagccaagt
    13861 caaaaaatct ggatctcgag aatctaatcg ttgcaggaga ttcagtaggt ggtaacatgg
    13921 ctacagtcat gacgatctta acaaaagaac gaaagggcct cccaatatcc aagcaattgc
    13981 tgtattatcc agtcacaaac gctgattttg atacagattc ctatcatgaa ttcggagaaa
    14041 actatttttt gacaagagaa ggaatgatgt ggttttggga ccaatatacg acgaatgaag
    14101 aagagcgtgc tgaaatcaca gcttcgcctt tgagagcaaa tataaatgaa ctaaatgatc
    14161 ttccttcagc aatgatttta acgggagaag cggatgtatt aagagatgaa ggtgaggctt
    14221 atgcgcgcaa acttcgtgat gcaggggttc ctgttacaca agtacgcttc caaggaatca
    14281 tccatgattt tgtcatggta aatgctatgg atcagacaaa ggcaacgcgt gcagcaatgg
    14341 cattatctac ttcttggatc aagaaaaaag aataagcttt gcttgtccgc aggaaaactt
    14401 cttttcaaaa acggattttc ttaggaaaaa tggcgagttt gactagtcaa tcacttgatt
    14461 ttgttttaca attaaaaaga aacgacagaa caaaggagag cttaaaaaat gggcaaattt
    14521 caagtgatcg atcatccatt gattcaacac aaactaacta ttattcggga caaaaactgc
    14581 ggaacaaagg tttttcgtga agtagtcgat gaaatcgcta tgttgatggc atatgaagta
    14641 tcacgggata tgccattgga agacatcgtg atcgaaacac cgatcactga aacaacacaa
    14701 aaaacgttgt ctggtaaaaa agtagcgatt attccaatct tgcgcgcagg tattggaatg
    14761 gttgacggta tcttggaatt gattccagct gctaaagtgg gacatatcgg tctttatcga
    14821 gatgaagaaa cattggaacc acatgaatat ttcgtcaaac ttccagaaga cattgatgca
    14881 cgtcagttgt ttgtagtaga tccaatgttg gcaacaggcg gatcagcaat catggcaatc
    14941 gatgcattga aagaacgtgg cgcgtcgaac attaaatttg tttgtttggt agcagcacct
    15001 gagggagtaa aagctttgca agaagcgcat cctgatattg acatctatac agcttctcta
    15061 gatgaaaaat tgaatgaaca tggttatatc gttcctggac taggggatgc gggtgaccgt
    15121 ttgtttggta caaaataagt aaatacgaaa aagggtgtga cacaactttt gtcacatcct
    15181 tttttggtca tttttaacgt ttgatgcaga acagcttttt cacagctttc gtttgcatat
    15241 aaaaaaacca tcccaaaaat gcagagatgg tcaaattcaa atctttttta gttctttgta
    15301 gttaacgatt actacaccct tactttgcgt catcaagttc gcattttctg cagtttcatc
    15361 aatctcaaca acagcggaat ttttcaattg ctttgaaatc gttccagttt gcgacttgcc
    15421 gtgaattgtg aacattacgt gagtattgat cttgaatttt tcaccaattt ccgatggtac
    15481 gatagttgta tcaaattttc caaaagttgc cattttcata aaacctgcct ttcacatcaa
    15541 ttataccacg ttttatgaaa aatatcatga aattaaacta attttccatc ggactcttta
    15601 caatcagcca aatagagtat ataatttctt ttggataagg ccagcttagc tcagttggta
    15661 gagcaacgca ctcgtaacgc gtaggtcgta ggttcgatcc ctatagctgg cattaaaaga
    15721 gaagacagat atatcaagga tttttaatcc attgatacat ctgtcttttt gtttaaagga
    15781 aataaagtat gctttcttag caatagagca tgtattattt ctataaaaat ttaggagaat
    15841 aaaacgacta gcaatatagc tgtgaaatca aatatcacat gaactgccca acttgtaatg
    15901 atcgagttgc ttttaatggc agcttggttg aataacaatc ttgccacccc ttgtatcaaa
    15961 aggatgtgtg ctacagtgtg taaaatattt ccattatcat aagtactgta atggataaat
    16021 ccaaagatga ttgctgacaa gaaactagcg acaggaatgc tgaacttact agaaaaaata
    16081 gcgagaaaat acactgagaa caattcttct ccaagtatca tgataggcaa aataataaat
    16141 aataacgggc tgaaagcctc atttattgga ttgcctgtta aaccccattt caaaatttgt
    16201 tgcaaaaata ccgagactgc ggcactgatt actacattga taaaaaaata tttggctata
    16261 tttttgacag gagcgacagg tttcgagaac atttttttcg tatctttttt accaaaaaca
    16321 agtgtaaatg ctataaatcc agtgatagtt aaagaaaaaa tgctaataat ggatggcgca
    16381 aatagcaacc ctaacaaacc gagtacaacg acaatagtgc ccaatatccc ttttttaagt
    16441 tctatttttt ttcctaaaat gttattcatg ataataactc ctactattca gtttttgaag
    16501 atagagtcag tatacatgaa gagttaaaat aggcaatgtt tttttatgga tatttgtatt
    16561 gctaaaaatt tatcttatat attcattttg attatactac ttttatttgt aaaaattttc
    16621 tagcaaaacc aagtgcttgc aactgattaa gcgtacaatt ttttagatgt tccgagcgta
    16681 cttcaatcat ttcaaaggag ttgctactta aatcgatatt ttttaaatca gtatcaaaaa
    16741 agctagcatt ttctaaatca ttagtagaaa acattatttt agtgagggaa cagtctacaa
    16801 aatctgcgct tgataagaca gagtgctcaa aggtactttc ttttatgttt gaaccagata
    16861 aatttacata gtccatttta caattctcga ataacgtgtt cccaatataa ctttccatca
    16921 atttagtacc tgttaattta caattgacaa atttcacgcg atgcaacatg ctttttgtca
    16981 atatacagcc tgaaaaatca cagttttcga atatacagtc ggtaatctcc attctttgaa
    17041 aatcattgga agcaaaaaat gcatacttga aaatagattt gtaaataatt ggatggcaga
    17101 ttatttggtt ttcgatgacc tctttttgtg aaaggatatt atacagttca aaatcttcgt
    17161 tttcatagat atgaaaaaag gatcctttat tcaagcttgc ttcattaact ttaggaaaaa
    17221 tcatatttaa ttacctccat tttcataaag tcatattaac aaaagctaaa ccaacttaca
    17281 ttagcagaaa atgagatagg ttttcgcctt tttcgataac gatagtgtcg ctatctcttg
    17341 aaacgttctt agaattgccc tgagagacac aaattaggcg taattctaat aaaaatctta
    17401 gaataaaaat ttttactgat ttaatttaga atgtttttaa aataaaaatc gttgccataa
    17461 cagtgtattg aaaggtttta acaatccagt aatgatttaa aaaaatctag atgtagcgta
    17521 tactttgttt atagaatgta gtgggaagaa atatattttt gggaggaaat cgtgatgtct
    17581 aaaagtgaag aaatagaaaa catggtacta aaattatatg atctcgtttt aaatccagag
    17641 atcaaagaaa aggaacgtat acttttgata gacgcaaaga caggcttaga aaaaggtcag
    17701 tattatccta aagtcataaa taatttggaa cgtagtttac gtccgttagc aatcagggga
    17761 gaattgtcta aaccagtatc accgttttat atggagatat caacaatagg aaaatttgaa
    17821 aaagaattag gtcgaggaat ggcttcggca ccgattactt ttgggcattt ataaaaacga
    17881 gttattcatc tcaatgaatt aaaaagtata gtcaatctca taatgcaggc taggttcatt
    17941 tatttgaaca gagtctgtat ttttttattc taaataaaga aacagatgtt tctttatatt
    18001 gtattttatc ctttaattaa cacaaaaaca gctagtataa actaataatg ttaattaaaa
    18061 gtgtatttta tgaaactatc gttatttatt aatgttgcat ttttgtaagt taaaaattgg
    18121 aaatatagat tgaaaa
//
LOCUS       DAJSBL010000054        17889 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.056, whole genome shotgun sequence.
ACCESSION   DAJSBL010000054 DAJSBL010000000
VERSION     DAJSBL010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17889
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.056"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(45..1511)
                     /locus_tag="ONT85_002166"
     CDS             complement(45..1511)
                     /locus_tag="ONT85_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HCR2379124.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
     gene            1898..2494
                     /locus_tag="ONT85_002167"
     CDS             1898..2494
                     /locus_tag="ONT85_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HCR2379125.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAGEKEYMNDADRFRVFG"
     gene            complement(2552..3034)
                     /locus_tag="ONT85_002168"
     CDS             complement(2552..3034)
                     /locus_tag="ONT85_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2379126.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(3140..3337)
                     /locus_tag="ONT85_002169"
     CDS             complement(3140..3337)
                     /locus_tag="ONT85_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2379127.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            3632..4390
                     /locus_tag="ONT85_002170"
     CDS             3632..4390
                     /locus_tag="ONT85_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HCR2379128.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
     gene            4450..4878
                     /locus_tag="ONT85_002171"
     CDS             4450..4878
                     /locus_tag="ONT85_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2379129.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            4924..4996
                     /locus_tag="ONT85_002172"
     tRNA            4924..4996
                     /locus_tag="ONT85_002172"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4957..4959,aa:Gln,seq:ctg)
     gene            complement(5146..5517)
                     /locus_tag="ONT85_002173"
     CDS             complement(5146..5517)
                     /locus_tag="ONT85_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HCR2379130.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            5732..5917
                     /locus_tag="ONT85_002174"
     CDS             5732..5917
                     /locus_tag="ONT85_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2379131.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            complement(6000..6188)
                     /locus_tag="ONT85_002175"
     CDS             complement(6000..6188)
                     /locus_tag="ONT85_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2379132.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(6212..6475)
                     /locus_tag="ONT85_002176"
     CDS             complement(6212..6475)
                     /locus_tag="ONT85_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2379133.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            6633..6890
                     /locus_tag="ONT85_002177"
     CDS             6633..6890
                     /locus_tag="ONT85_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HCR2379134.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            complement(6961..7152)
                     /locus_tag="ONT85_002178"
     CDS             complement(6961..7152)
                     /locus_tag="ONT85_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2379135.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(7293..7613)
                     /locus_tag="ONT85_002179"
     CDS             complement(7293..7613)
                     /locus_tag="ONT85_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2379136.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            complement(7636..8070)
                     /locus_tag="ONT85_002180"
     CDS             complement(7636..8070)
                     /locus_tag="ONT85_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HCR2379137.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            8405..9043
                     /locus_tag="ONT85_002181"
     CDS             8405..9043
                     /locus_tag="ONT85_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HCR2379138.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNSISDINLIYSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPV
                     QAEQPVVQETVQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTAT
                     NGRYIGRYQLDSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
     gene            complement(9367..9450)
                     /locus_tag="ONT85_002182"
     tRNA            complement(9367..9450)
                     /locus_tag="ONT85_002182"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9414..9416),aa:Leu,seq:cag)
     gene            9978..10478
                     /locus_tag="ONT85_002183"
     CDS             9978..10478
                     /locus_tag="ONT85_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR2379139.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            complement(10668..11012)
                     /locus_tag="ONT85_002184"
     CDS             complement(10668..11012)
                     /locus_tag="ONT85_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HCR2379140.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(11166..12086)
                     /locus_tag="ONT85_002185"
     CDS             complement(11166..12086)
                     /locus_tag="ONT85_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HCR2379141.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            12273..12764
                     /locus_tag="ONT85_002186"
     CDS             12273..12764
                     /locus_tag="ONT85_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HCR2379142.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            complement(12788..15163)
                     /locus_tag="ONT85_002187"
     CDS             complement(12788..15163)
                     /locus_tag="ONT85_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HCR2379143.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            15489..16115
                     /locus_tag="ONT85_002188"
     CDS             15489..16115
                     /locus_tag="ONT85_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HCR2379144.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            16138..16842
                     /locus_tag="ONT85_002189"
     CDS             16138..16842
                     /locus_tag="ONT85_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HCR2379145.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            17034..17867
                     /locus_tag="ONT85_002190"
     CDS             17034..17867
                     /locus_tag="ONT85_002190"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HCR2379146.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
BASE COUNT         5553 a         3489 c         3015 g         5832 t
ORIGIN      
        1 aggaactgct gcaaataagc aattcctgtt tttatgttca ttgattaatg aatagagccc
       61 attagttttt tgattggttc tttcacagta aatagcaaga taccaccgac gattgctact
      121 gcaccgataa taccgaagta agcagattct gttcccggtg tgtagaagcg tgcaatttgt
      181 gcattgattg cttgtgaaga agcatcagct aagaaccaaa tagcgattgt ttgtgattca
      241 aatgctcgtg gtgctaattt tgttgtcaca gacaaaccaa ctggtgaaag acacaattca
      301 gcaataacca cgatgaagaa acttcccaac aaccacaatg gacttacttt tccacttgta
      361 ccataaagca tacccggcaa catcaatagc aagtacgata atcctgcaaa taaaagtcca
      421 agagagaatt tcactactgt agatggttga cgttcaccaa gtttcgtcca catcgttacg
      481 aatactggtg tcagaataaa gatgaaaatt ggattcaatg attgatacca gcttgcttgg
      541 atcgttacac caaatagcgt agattgtgta cgttcactag caaataaagc taagatagat
      601 gaaccttgtt cttgtaatga ccagaaaatg attgctgcaa ggaataatgg gatataagcc
      661 catactcttg gtttttcttc agcagttaca tcttttgaag tcagcatttt gataaagtaa
      721 tacaacggta agataatccc caatacactg attgtattga caaaaaagtc aattgtcaga
      781 tgaccagtta gttgtgcccc gccgaatact gcagcgataa caacaagtgc gatgatcaag
      841 ttacggatga atcctttacg ttcttcagtt gtcaaaggat tcggcggtgt tgtacccact
      901 ccgtctaatg tcttgcgtcc ttggatgatg tattgtaata atccaaagaa catcccaata
      961 gcagctaaag agaaccctaa atgatagtta tattcttgac cgattgtacc aacgattagt
     1021 ggtgctaata atgcccctaa gttgacaccc atgtaaaaaa tcgaaaatcc tgcatctctt
     1081 cgcaagtcag tctttgaata aagatgtccg accatgccag aaacatttgg tttcaaaaag
     1141 gcggttccga taataatcaa tgccatcgaa acaaacaatg tacttacacc aaatggtaaa
     1201 gccaagacga tgtggccaag cataatgaaa actccaccga agaatacagt gcgttttgct
     1261 cctaacactc ggtcagatac ccatccgccg acgatactag acatgtagat caaagatccg
     1321 tagatcgaca tgatagctaa tgctgttgct cgtggtaatc caagtccccc gttggcaact
     1381 gtatcgtaca tataatagat aagtaatgca cgcataccgt aataactaaa tcgttcccac
     1441 atctcagtga agaacaaagt tgacaatccc ttcggctgcc cgaagaagcc cttgtcttgc
     1501 tgtttttcca taatctatac cccaattctt taaattttca gaaaatatta tacatcttta
     1561 ccgaaaaatt taactaaaaa acttatttaa gaaatataaa ctaattttct ataaaagcaa
     1621 gtaattttta atggcaatat cataaaaaaa agaagataga gatttaataa ttatatatca
     1681 gctgtttttt tcaaataagc aaaaattcct aaaacacaac aatgaaagcg ataactaaga
     1741 aaatccttac ataacgattt ttcttattaa aattgttttt tgaacttcgc ttattttttg
     1801 tgagcatatc tgtttactta ctttttgtaa ggtgctatac ttcatttcgt aatcaattaa
     1861 aaaaatgcaa aacgcaaatc aaaggagaaa ttttattatg acaactttta ctgacacttt
     1921 gaaaaaccgc cgttcaatct atcatctagg acgtaatgtt tcactttcta atgaagaatt
     1981 aacaactttg atcaaagaag caattaaaga aagcccaact gctttcaatg ctcaatcaac
     2041 acgcgcagtt atccttttcg gagatgcaca tgaaaaactt tgggaaatca ctgaagaagc
     2101 acttcgccca ttgactccag cagaagcttt cccaaataca caaaacaaat tagctggttt
     2161 caaaaatggt tatggtacag tattattctt caaagatact gacgtggtaa aaggtctaca
     2221 agaacaattt gaattatatg cagacaactt cccagattgg tcagaacaat caaacggaat
     2281 cgcaacagct aatacttggg tagcattagt tgataaagga ttgggcgcaa acttgcaaca
     2341 ttacaaccca gttattgacg aagcagttgc aaaagaatgg aatatccctt caaactggaa
     2401 actacgttcg caattagtat tcggttcacc agaaacaccg gctggggaaa aagaatacat
     2461 gaacgatgcc gatcgcttcc gcgtatttgg ataattttat ttgaaataaa aaacagaggt
     2521 tgtgacaaac atcttgtcgc aacctctgtt tttattctgg atcaatcata atatcaatct
     2581 ctgcctcatc gtttaagcgt tgggacaaaa atggaactaa ttcttctaat gatgcagaaa
     2641 atttcagcat cggtcctcgc catccgtttc ggaaaaaatt catttttacg attttagaac
     2701 cttttggctg atataataca attttattga tctcagaaaa aggaacacct tttttgtcaa
     2761 agctattcaa aacaagtcct tcttctgtga ttcctctact gtccaacaaa aaactaataa
     2821 aaatcattgc tgacaagatc ccttttgcca attcatctgc tgtaggatga ctgatgagtg
     2881 tataccaaat gactagtgac actaagaagg gtatcaccaa gtagacccat cgatttcgca
     2941 cagtccgata aataatttct tttcgaacca gccaaagaaa taaaaacatc acaacaacga
     3001 ataaaaacaa tagaaccgta aagaatgtta ccatgacaac tcgcctcgct ttttcttctc
     3061 cagaaaatag gaaaagaata ctgatttacc ttctcaaaga aattggactt gcagcacgcc
     3121 gcacgtccaa ttgttttctt tattcaaatg tatcatattt tgcaacaacc atgcttgctt
     3181 tttcatctaa taattcttgg tcctctgcag cacattgggc aactttgacc acggcgcagt
     3241 ttgtcatttt tgatacaact tcaccaatga ccgtttttgt aaaaccaatt ggtttgcatg
     3301 catattgttc tcctaaatta atcatttctt ccgtcatcaa aatcctccta aactgctgga
     3361 acttttctcc acattaccac tgctatttct ttggagattt aactcattga tttttattta
     3421 cagttaaaaa tcaaccttac aggcattata gcgtactgtt ttcgttttca caattaaaac
     3481 agctgttgta tttagaagaa tttttttcaa aaaattaaaa catattcctt aaaaactaat
     3541 tatttcctac atattaaaat ataatacttc catttccaat aaaaatacat ttataatagg
     3601 aaagataaac agctgaaaag ggagatttca aatgaagaaa aaaacgttca aaaaactatt
     3661 gtttctaggg ggcacagctg ctcttttatt aagcggtaca agttattacc tacatatcca
     3721 ttctccagaa catacaatga acactgcaca aaaaagttca acacaaaaaa cgaaaaagac
     3781 aaaagagaaa gcagaagttt tactagatgt tccattagag agccagtttg attctccttc
     3841 tttagaaaat ggctgtgaag tcacttcgct ttctatgctt ttatcttttt atggttatga
     3901 aacaactaaa aaccagttag ctgagcaatt ggattacgtc cctgttttca atgcagatgg
     3961 gactcatggc gatcctaatg aaggatttgt tggtgatatc agtggcggag actgggctat
     4021 ggtcgtctat gttcccccag ttgcctccct tgctcagcaa atcgtccaaa cggattatca
     4081 cacttttcca aaaaccgatg ctcaactcga tgatatcaaa aaagcactat ctaaaggaaa
     4141 tcctgtctgg acttctgtga ctatcaattt tgaagtccca gatgaaactg attttatgac
     4201 ttggaccaca aataatggag aagtaagagt cactcctctt gttcatgctt gtgtagtcac
     4261 tggatatgac aaagaaaata tttatgtcaa cgatccctat ggtgtaaaaa atcgagcagt
     4321 accgatcaac gatttttcgg cgattttcac tgcaatgggt ggacagatgc taactttgga
     4381 gaaaagctag taattcaaac aaaaatccct tatactcaag ataccaaacc tttgaaggga
     4441 gcgtagataa tggaaaaatt cacacataaa aaaatggatc caaatgaaat tccgattatt
     4501 tttgtccgtg actgcaaggg aaatgttcaa ggtaaagtct ctattaatga gtggaacgag
     4561 cgtcgcagac ctgctaccct caatgaactg gaaatcaaat tgtatcgtca atctcttgtt
     4621 tattacgcag atcaagagta cgagaaagcc accgatttat tgaaatttct aattgctcga
     4681 acagagtata ctcgctttga atatattgaa cgtttagcaa acatctatca tattatgaat
     4741 gaacctgtca aagaatatca gttattggac accgtcttgt cagtagcaga actgattgcc
     4801 cttcctgctg gattagaaaa gaaactagta cgccgccttt tacgagtaaa gcaacaacta
     4861 tccgatcaag aaaaataact tgcgtttttt taggaaagtg ctatgattat tttgttgaat
     4921 aaatgggatg tagccaaact ggtaaggcac ttgactctga atcaagcaat tgtaggttcg
     4981 aaccctgcca tcccaatttg atgtgcttgc tgaaaaaatg aaccccctgt aaaagaacaa
     5041 tctttttgca gggggattat tgtattattt ttactacttg tatttagttt acataattaa
     5101 attatgaata ataaaatcgg gcgtattctt aatacaaaaa ctgatttacc caataattgc
     5161 taatgctttc tccggaactt tttctctagg aacttcctca tacgtttcta tgtgtgctcc
     5221 tttattgcta attttcaaat aatgattttc ttttaacaca gaaagaccat taaattcaac
     5281 cgttcgttct ttcccattgg catcagcagc tttctgcaca taccttgctg taccatattc
     5341 atttacttcc tctggttttt gtgtctttac atagacctct cctttttcaa ctaatggatt
     5401 tagctgatca agtataccag ggatttctcc ataacttcct tcagtataat atttcagtcc
     5461 aaatagcaaa ataccactaa tcgccgccag tccaatcaaa tatttaatca ctttcatctt
     5521 tctacctcct tgttatatat tcagtatatg ggtcagttaa agaaattcca tccgtcccaa
     5581 gttggagatt tttctcctac tttagaagcg agaatcgagt gtttttttag taaaaatgat
     5641 cgttatttga tattattgac gtgtggataa atcagttttt ctcgttttgt ccatgtaaaa
     5701 aatcatgttg atagcaaagg atgtgttcaa aatgaatcaa gaagaattat ttcaaaaagt
     5761 caaggaaatg attaagaacg gcaatttcga tggcgcaaaa cgatttatcg aagaacataa
     5821 agaacaatta ggaccttata aagagaaagc ccaaaatctt ttaaaagatg taaatatcga
     5881 cagtgtcaaa aacaaattta aaaatctatt caaataagta aaactttcac ttttgtaaaa
     5941 tcaaaggagg acaatgctgc tgcatcgtcc tcctttggtt cttttctgaa tacttcatac
     6001 taaaataaac gtgtcggatc gtaatgataa gtcacattgc cgttcagtga ggcttttccc
     6061 accacatatt tacgattctc caacttcagc cacgcttctc gaaaagccat taattcttct
     6121 ctttttacat caatcttttc gatttttcca tcgatcaaat cttgaataag ttgttcatat
     6181 ggattcattt ttttcctcca tctgctgtca atcaataagt tgtaaaaata ggttcatatg
     6241 cttttttggt atctagatct tctctgactt tgactgtcgt ataaataatt ttttctactg
     6301 ggatactata ataacttcct tcaccattat tgaatcggtg aacgtctttc tttgctaaaa
     6361 taccatttat tttattcgtc agtacacgtt gttcgacact gttacttaca acatagtaaa
     6421 tcacccgaat attattgatc aaatgcaaat cgatttctaa cactacatga tccatgaaaa
     6481 tcccactcct tctttcactt tgattatagc aagtttctcc tctaatttca aaatcgatac
     6541 agattttacc ggataaatcc acaaaactgc ttgcgccatt tcgcacaaag tgttattttg
     6601 tatcatggac tttatttatc ggaggcaata gtatgatcgc gatcggtcaa tttgtttttt
     6661 atattccttt ttttattatg ataagcattc ttttctatta cataaaatgg acaaaaaaga
     6721 agttttcagt tttactggcc tctttacctg ctgtatattt tacttatcaa attttttctt
     6781 ttaggcattg ggaaacaacg tctgtattaa taacacacat catcgaattg acgctttcag
     6841 taatttttct tattatttgg atctattttt tatataagaa tcagaattga atccctcgca
     6901 tgtttaaaaa gaaaacgatt aaagatgcag caaatttctg ccgatcttta atcgtttttt
     6961 ctattctgtt ataccaaaaa aatgtttgaa tcggtgaagg atcgttcctt gttttaattc
     7021 tataaattct tgtgcgatcg ggtacttttc ttccaaattt tctttgttct tttcgatcag
     7081 gcttttcgct tcttcatatt ttttatctgc acatagtgct tttacttcct gtaatacttt
     7141 tcctgcttcc atttgcctca ccctcttgcc attttattgc tttaagtttt atttaagtta
     7201 tacgcattat tctagtttca tcccaaataa ccttttttca tttttactcc tcgaactaat
     7261 cagttttcta aaggctgatt ggttcgtctt ttttattttt cctccatttc attctgctct
     7321 tctgttaatt tatctaatgc tttgattgat gctgcggctc ttgcataatc atagctgtat
     7381 aattccataa atttatcagg cagaacaaac cacgctggat gataaccgtt agctgtgaca
     7441 gggaatcctt tatcttgttg gttatctcca tcccatctca gagcaacacc tttttttcct
     7501 ttccacttta ctaaagcgat cgagtatccc ttctttgttt gtgtgccgtc ttcaataatt
     7561 gctaaaattt ctattttttc ttttggcgca cggacttctt gaggacgaac cattttctat
     7621 tcctccctta tctcattact tcacgaccaa cacactgcaa ggggcatgtg atacgacata
     7681 agaagcggtc gatccaacta atgcttgttg tatagcacct tttcctgtcg ctcccataac
     7741 gatcaaatca atattttctt gtccagggat gacattggca atcaaatttc ttggatctcc
     7801 agtttcaact attgcgtgga tctcagcaat gccgtactct ttggcatcat tgattttttt
     7861 cagcatggct acttctgttc tttgtttttc atctgcatag atttttgaaa aggcataggc
     7921 actatgggtc agctcaactt tgttgatgat tgacacgata aataagtggg cttcattgtc
     7981 ctttgctaag tcaagggctt cagcgaacgc tttgatcgat tgtcttgatc cgtcaactgc
     8041 taccatgatg ttctggtatc ttttactcat tggaaccgcc tcctttttct tttattgtaa
     8101 catctcaagc aaaaatacaa aaaatatacg caatatgtaa ataaaatgat gaacaaaagc
     8161 aatttctttc aagttattta tacatttctt ttctcatcag aacacctgtt ctaatcccat
     8221 tatttctgca ttatgtgaaa ttcctcatca tttttcacat cgagcgaaca tcattctgtt
     8281 acaaaaaacg tggatttgtc ataaattgtc atgcttcttt tacctatgaa atattttcac
     8341 gtgttaaagt attcctcgtt gaacacaaca aataaaactt tttaacgaat aaaggagatt
     8401 tttcatgaca tcacttaaaa ctttactttt tggaacaact ttggctgccg gcgctgcatt
     8461 cttcatggga acgactgctc atgcggacga agcttatacc gttcaatcag gtgatactct
     8521 atcaacgatt tctcaaaaat acgttggtga taactccttg atcaatgcca ttgcagaatc
     8581 aaattcaatt tctgatatta acctgatcta ctcaggacaa caattaacaa tcccaacaga
     8641 aggctctgca caagcagctg ctgaacctca agcagccgtt caagaagcac cagtccaagc
     8701 tgaacctgtt caagctgaac aacctgttgt acaagaaacg gttcaaacgg aaacacaagc
     8761 agcgcctgtt gctgaaacac aacctgctcc tgctgtaaca gaaactgctg ctactccagc
     8821 aagtacaagc tctgcaaaag aatggatcgc acaaaaagaa tcaagcggtt cttacacagc
     8881 aacgaacgga cgttacatcg gccgttacca attagattct tcttacttaa atggtgacta
     8941 ctctgctgca aaccaagaaa gagtagcaga acaatatgtt acttcacgtt atggttcttg
     9001 ggaagcggcg aaagcattct gggaagcaaa cggttggtac taagattcat tatttgaacg
     9061 tttaaacaca ttcaaaatat agtaagaggt catgcgattt attctttcgc atgacctctt
     9121 tttgagttta agtttagtct ttattcctta actatcgttt gccagtattt cttgtatcag
     9181 aatctataaa tctactattt gaatcaatct attaggtttt aaactgtccc accttcacac
     9241 aaattttttt tgatttattt gaatctggtt ccgttgttta aaatttttcc tatattcttt
     9301 tagataatat gttattttct ttctcatcgc tccaggtgta ccttctcttt ataaaaaaac
     9361 aaattatgcg gctaagaaga ctcgaacttc cacgaggtca ccctcactag cgcctgaagc
     9421 tagcgcgtct gccaattccg ccatagccgc atcacatctt ttagtttagg attacatgtg
     9481 ttttttgtca agaacatatt tttgttcttt ttcatttctg tacgccctcc cttatgccat
     9541 ctacacaaat aattcatccg agcaactcca tcatctattg agggttggtg aaaaaatctg
     9601 ttttatccat atgatcgatt tcaatgttaa acgaacgact ctgggattag taactatttt
     9661 atttttttct tcatttcttt tttcctcttt ttctcgattc agctatcttc tttcatttgg
     9721 atattgatta tttcttctgt tgattactat ccttctggaa ttctagacgc tgaagtaatt
     9781 cacgttttct ttcttctaga gatcataagt aacgaatgaa cgaacttcaa ataatttttt
     9841 tattgtaatt catatttgta ttgttcgatg attcttaata cgatatgaca caataaataa
     9901 aaataggcat ggctatttac aacaatagtg ctatagtaga aaacaaatga ataaggttac
     9961 ttaaagaggt gaatacgatg aaactgatca aactatggga agcagattta aagaaagctt
    10021 atcagctaca aactagcttt ggaccaaatg agagtggatt tatcaacaat gcttatggct
    10081 atacatttgc tgaattttta gattatgtaa aaaaatgcga aaaaacgtct ttaggaatcg
    10141 atcttcctga gagttacgtt cccgcaaccg tctttatttt agaaagtgat gaacatgagt
    10201 atgtaggaat ttttaatttg cgccataaac tgaacgaaac attaagaaac ggcgctggcc
    10261 atattggtta cggcatctcc cctaattatc gtaaaaaagg atatgccaca caaggattag
    10321 cattagtact acaagaggca aaaaaaatcg gcattgatga ggcacttttg tctgtaaata
    10381 aaaaaaatct tggcagtctt aaagcacaac aaaaaaatgg ggctgcgatt tatcgtgaag
    10441 atgagaaaga atactatacg aaaatcaaac tgatgtaaaa aagaaggatt ccaaaaggaa
    10501 agggatttga atgtccttta tcaatagaac acatttattt aattaactta taatatagca
    10561 gacaaaattt tataaacaaa gaaacctttt atctcttttc ttctagaaag gataaaaggc
    10621 ttttttgttt ttcctatttg cttctcatag ctagtcagcc aagatttcta tcgtatcccc
    10681 aacgcaatcc gtgcataacg gctcatctta tctgtcgtcc atgctggata ccagactagt
    10741 ttgacttctg ctttcgttac ttctggcact tcttttaatg cgtcatggat agattctgtt
    10801 aaaatatctg ctaatggaca gcccatagtt gttagagtca tcttaatgac tgtttctcca
    10861 ttttcaggat tgaattctat atcataaatc aagcctaaat tgacgatgtc gattcctaat
    10921 tcaggatcaa tcaccatttc caaggcagca agaatacgtt ctttgattag ttctatttct
    10981 tgtccagacc gttcttggtt tgcttcgctc atcgtacgac actccttaac acgattttta
    11041 tttatcatac tcttttttta gctgcttgta aatgagaatt tagaagtaag attctcgttt
    11101 aaatttataa aaatgttctt attagatgta atagattaaa tttattctaa aataaacctg
    11161 attcctcatt ttttttgtga acgatttttt atttctggca atacgccagt tgttcgatac
    11221 attttttgtt cgttttcatt tgcaaaccgg ccaaatactg aatgagttgg taaaatattg
    11281 ataaatgcat cgtcgtcgat ttcatgaacg atctgatcaa gatcatacat ttcgtagcgg
    11341 gtgatgacca tcatgatcat gttcccttgt ttttttgaat agccaccgac agacggcagt
    11401 aatgtcattc cacgtaccat ccgttttgct agttcttctg ttacctcttc tggcttgacg
    11461 gtaacgatca ttgctgttac tttttgatga ctcgtgtgga tggcatcaat tacttgagtc
    11521 attgcataaa tggaaataat cgtatacaag gcactttccc agttaaagaa gaagcctgcg
    11581 atgactacaa tgataccatt tagaatcagc atgtagtttc cgactgtttt tccagttgtt
    11641 tgtgaaagta caagagagat gatatccatc ccaccagttg taaagcccat ttttaaagat
    11701 aatcctacgc caagacctaa taaaacaccg cctactaggg cattcatcaa aatattatca
    11761 gtgatctgtc cgactggaag cagcatcgtc atcaccgata cacatacaac attcacaaaa
    11821 ctaaggatcg ttgctcgttt tcctactttg agaaacccta atacaaacac agggatattc
    11881 aaaagaaaaa taaatgttcc tgtgtctatc ttgatatgaa accattcata taaaagcgag
    11941 gaaatgattt gagcgatccc attcatccct gcagaaaaga cattggctgg aatcaaaaaa
    12001 taatttaaag cgaccgcagc ggtcaatccg gttatcaaaa taaccactat tttctttaat
    12061 ctttcatttt tatgatacgc agtcataatt ttactcacaa gcacctctcc cctactttgg
    12121 tattccaaat gtaagaataa cagtttatct attaaaataa aatacttcga actcaaatga
    12181 gaagaatttt agcaaacagg taagaatctg ccctaatcct atgataagat agatgtatgg
    12241 taaatattta tatgtatatc aggagggttc ctatggcaaa agatgctagc ttcgacattg
    12301 tatcagaagt caatacagaa gaagtaaaaa atgcgattca gcaaacaaca aaagaattgt
    12361 ccaatcgctt tgacttcaag gattctacag tagaaatcaa attagagaac agcaatctag
    12421 ttgtattgag cgatgatgat ttcaaaatcg aacagatcaa agatgtatta tttggtaagc
    12481 tgatcaaaag aaatgtgcca atcaaaaata tccatttttc cgaatcagaa cacgctctag
    12541 gtggcaaagc tcgacaaact gccgatttgg tcaacggaat cgatcgtgaa aacgcgaaga
    12601 aaatcactac agctatcaaa aatgaaaaac tgaaagtaaa agcacaaatc caagaagatc
    12661 agattagagt gacaggtaag aaccgtgatg atctacaagc tgtgattgct ttattacgta
    12721 aacttgactt accaatcgag ttgcaattca ctaactatcg ttaatcaaaa aacgaggctg
    12781 acgtctgtca ttcctcgttt ttttgattta ctgcttcttt tgataaccaa aaacgctgta
    12841 ttccttcttc caattcccca acacttcgta cttctcttgc tgtttgccaa tgttgtggga
    12901 ataaacgtcg ataagaaaaa ctagaaaatc gttgatctgc caatacaacg attccttgat
    12961 ccgccatgcc acgaatcact cgaccaccag cttgcaagac tttgttcatt ccaggaagct
    13021 gataagcgta agcaaatcca aggtgttcct tctcatcata atatgatttg atcagttcct
    13081 gctcatgatt catctgcggc aaacctacac ctacgatcat actgccgatc aatcggtccc
    13141 ctctcaaatc gattccttct gaaaaaatcc cgccaagtac gcaaaatcca atcaatgttt
    13201 cagcgggatt tgtttgaaat tttgttaaaa atcgttcgcg ttcttcctca ttcatgtccg
    13261 tttcttggat tagcacattg ctgtcgggat attgctgtct gaatgcttcc accgcaagat
    13321 ctaaatattg ataagaagga aagaaaacga aataattccc gacctttcca tcagcaaaag
    13381 catgaatcgt ctctactaat tttgccagac tgtgttccct ttttcgataa gtcgtctcta
    13441 ggtaatttgc gatgaaaact tgctggtttc cttctgggaa aggactcggc agtttgtaag
    13501 ccagactttt ttgtcccccc agtgtttcct gataataaga gagtggtgaa aaactagcag
    13561 aaaaaagaat actgcttttt cctttatcca acgactgctc aagaaacaag cttggatcaa
    13621 tacaaaactc ttttactatc agatcgcgat acgttttttc gactgttgtt tcgtagtgat
    13681 catcataaaa ttcactgacc ttcaaaaaat gaagcaaatc aaaataataa ggaagcaatt
    13741 gttcttgtga tgggtgctca ggaaattctg ccagccattc tttaattttt tcgcttaatt
    13801 gataacctgc ctttactaaa gattcagccg gtgctttttg atgatgataa tcccaatgat
    13861 cttcttttgc tatttctcgg atcgaatcaa attcttttaa tagtttgttg aaacggcgat
    13921 gaagtttttt gaattccttt ggtatagctt ccttacttcg tttcgttttt tcataagata
    13981 gttctgccga atacatctct cgggaacgat tcaccaaatt atgtgcttcg tcaatcaaaa
    14041 acagatagtc ctcgtttttt tcatcttcaa aaaaacgtct taaataaaca gttggatcaa
    14101 ataaataatt gtaatcccca atgattacgt cacaccagat actgacatct aatgatagtt
    14161 cgaaaggaca aagtgtatgc tttcttgcat aagtttcgat gacttcacga gtgatctgat
    14221 tctcatgatt caaaagatcc cataaacctt cattaatccg attgtaataa ccattggcat
    14281 atgggcactg atctggatta caggtcgtct catctaaaaa gcagatcttg tctttcgccg
    14341 tcaaagtgac actttttact tcagctcctg tatcttttaa tgctgtcata gcatcttcag
    14401 ctacttgtct agttattgtt ttagccgtta gataaaagat cttttctccc tcttcttccc
    14461 ctacagcttt caaagcgggg aataacgtag agattgtttt tcctgttccg gtaggtgctt
    14521 ctacaaacaa tttctgtttc gtacggatcg ttttataagc agctaccgct agttcacgct
    14581 gacctttcct atactcatca tgggggaatt tcaaagccat caaagaggca ttgcgtacag
    14641 tgcgccagtt ttcttgaaaa acaagccatt ctttataatc ttcagtcagc tttttaaaaa
    14701 aagtttctaa ctgctctctt gtctgatgtt caacctttct cgtgatcact tcttctgccg
    14761 tctggaagta agtcaattgc agatttattt cttgtaaatc atgttcttga ctatagatat
    14821 aggcatacac ccttgcttga tgaaagaaaa gttccacttg ttctggttct aaatcttcaa
    14881 atcgtggttc ggaggttttg atttcatcta tataataaat accgtctctt ttgaaaatac
    14941 cgtctgcacg gccttcaacg atcaggtaat attcatctaa ttgaactgtt gtttttaaaa
    15001 agacttcttt ttcatagttt tctcccgcat ctttttggag cttgcggtgg attctcgctc
    15061 cttctaatgc agtatggtta ctttttttac gattgtcgat acttccacgt cgcaaaataa
    15121 attctactaa gtgccgcaca gatatttttg actgtttgat catttccacc acctctttct
    15181 ctctaagatt acctgaaaaa acctagttta gcaaaaagag ctctctttcc aaacataaaa
    15241 aaacaccctg ctgattaggc agggatatag gatatagaat ttggattttt ggatgaatct
    15301 aatgtaacaa ggacatatta aaatgctatg aaaacttact ttccttctct ttagaatatc
    15361 caaatacttt tcatatagtc tgataattca gataagtaaa gagaaataag tacctgtttt
    15421 tgtttcctag caatgaatgc gttatactaa tggatgaaag aagttttggc tatcaaggag
    15481 gaatgcacat gcatatcgaa agattaaaaa caggaatgat tgaagaaaac tgttatttgg
    15541 tctacaacga agaagcactt ttgatcattg accctggaga agatgcggaa cgaatcaaac
    15601 aagaaatcaa aaaaacaaac caacagccgg tagcgatttt gcttacccat actcactacg
    15661 atcatatcgg tgcagtggaa cctttacgcg cgtattatca aatcccagtt tatgtcagcc
    15721 cactagagca aaaatggcta ggtgatccga ttttgaatct ttctggactc ggtagacatg
    15781 atgatattgc tgatgttatt gtctcgcctg cagaatatga atttgaaatg aaaacttata
    15841 ctttaggaaa catgtctttt cgcgtcgtcc ctaccccagg gcattctata ggaagcgtca
    15901 gctttatttt tgatgatttt gtcgtttctg gcgacgccct gtttagagga agtatcggta
    15961 gaaccgatct gtatacaggg aatttagaac aattacttca tagtattcgg acccagctgt
    16021 ttgtattacc tgatgaattt gcagtctatc caggacatgg cgatgcaacg acgattgaac
    16081 aggaaaaaag gacaaatcct tttttcaatc gttaaaacga cttaggagga gaataaaatg
    16141 acagaaacta cactttatat cgtacgacac ggaaaaacga tgttcaatac aattgaacga
    16201 gtacaaggct ggtgtgatac acccctcaca aagcaaggac aagaaggcat tcattattta
    16261 ggtcgcgggt taaaaaatgt cgacttttca tttgcttatt ctagtgacag tggtcgtgca
    16321 atcgaaactg cccgtattat tctaggagaa cattctaagg gaaaagaaat tccttatttt
    16381 attgaaaaaa ggattagaga atggtgtttc gggtctcttg aaggtggata tgacatggaa
    16441 atgtggggtg tgatccctcg tgtattgaat tttaaaacat atgatgaaat gtttaccaca
    16501 gaagtttctt ttgaacaaat tgctaatgct gtatacaact tagataccgc tgggtgggcg
    16561 gaaacttatg aaacattgcg agaccgtgta tggacaggat ttgaagatat cgcccatcac
    16621 tgcgaaaaaa atggtggtgg aaatgtttta gtcgtatctc atggtttgac gatcgccttt
    16681 ttgcttaatc tgatcgatcc taaacagcct gttcgtgctg ggcttttaaa tggcagcgta
    16741 acaaaggtca cttatgcaaa cggtcgattt tctatccacg gcatcaatga tacgtcttat
    16801 atcgataacg ggaaaacaca tagtcgcgac tcgtttctat aatggggata ttttaatttt
    16861 taccagagaa tcataaaagg caaagtctga acgagggctt tgtcttcttt tttctcttcg
    16921 tgtacttatc tgctttattt ctcaagtttt tcttaatatt aaaaaacaga ttgggtttct
    16981 caaaaaataa cggtatacta taagatgtat cttaattggg aaggaaggaa tttatgtttt
    17041 ttgcaaatat tatcaaagca gtgattctcg gtattattga aggaataacg gaatggctgc
    17101 ccatcagtag tactggtcat ttgatcctag cagatgaatt catcaaactg gatgccagtt
    17161 cacaatttat gtcgatgttc aatgtcgtta tccaattagg ggcgatcttg gctgtcgtcg
    17221 ttctttattt tcacaagtta aacccatttt ctccaagtaa atcaacggta gaaaaaaaag
    17281 atacgtggac actttggttt aaagtgatcg ttgcttgcat accagccatc atcattggtt
    17341 tgcctttgga tgattggtta gaagcaaatt tccacaagtt cttaccagtt gccattgttt
    17401 tgatcgttta cggtattgcc tttattattg tcgaaaaacg caacaaaaac aaaacacctc
    17461 gttggacttc tctgaatgac tttacgtacc aagcagctct tcttgttggg ttattccaag
    17521 tcttggcact tgttcccgga acatcccgtt ctggcgcaac gatcctagga tctatcttga
    17581 ttggtgcttc gcgttttgtc gctacagagt tttcattttt cttagggatt cctgtcatgt
    17641 ttggcgcaag cggcttgaag atcgtcaaat atttagctga agggaattca ttcaaaatgg
    17701 aagaaactgt tgttcttctt gtaggaacat tcgtatcttt tgtggtttcc gtcttagcta
    17761 tcaaattctt actaagctat ttgaagaaaa acgactttac cgcttttggc tggtatcgta
    17821 ttatccttgg tatcattttg attggttatt ggtttattgc aatgtagaaa aaaccagaac
    17881 acttctctt
//
LOCUS       DAJSBL010000055        17225 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.057, whole genome shotgun sequence.
ACCESSION   DAJSBL010000055 DAJSBL010000000
VERSION     DAJSBL010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17225
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.057"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            2..370
                     /locus_tag="ONT85_002191"
     CDS             2..370
                     /locus_tag="ONT85_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HCR2379147.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            550..1491
                     /locus_tag="ONT85_002192"
     CDS             550..1491
                     /locus_tag="ONT85_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HCR2379148.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            1560..2291
                     /locus_tag="ONT85_002193"
     CDS             1560..2291
                     /locus_tag="ONT85_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HCR2379149.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            2435..4615
                     /locus_tag="ONT85_002194"
     CDS             2435..4615
                     /locus_tag="ONT85_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HCR2379150.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            4635..5117
                     /locus_tag="ONT85_002195"
     CDS             4635..5117
                     /locus_tag="ONT85_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HCR2379151.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            5133..5894
                     /locus_tag="ONT85_002196"
     CDS             5133..5894
                     /locus_tag="ONT85_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HCR2379152.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            6025..7359
                     /locus_tag="ONT85_002197"
     CDS             6025..7359
                     /locus_tag="ONT85_002197"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HCR2379153.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            7666..8151
                     /locus_tag="ONT85_002198"
     CDS             7666..8151
                     /locus_tag="ONT85_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydratase"
                     /protein_id="HCR2379154.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            8144..8827
                     /locus_tag="ONT85_002199"
     CDS             8144..8827
                     /locus_tag="ONT85_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HCR2379155.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            8953..11598
                     /gene="polA"
                     /locus_tag="ONT85_002200"
     CDS             8953..11598
                     /gene="polA"
                     /locus_tag="ONT85_002200"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HCR2379156.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            11643..12479
                     /gene="mutM"
                     /locus_tag="ONT85_002201"
     CDS             11643..12479
                     /gene="mutM"
                     /locus_tag="ONT85_002201"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HCR2379157.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            12443..13072
                     /locus_tag="ONT85_002202"
     CDS             12443..13072
                     /locus_tag="ONT85_002202"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HCR2379158.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEKLVGQIENWLFANRFL"
     gene            13124..13456
                     /locus_tag="ONT85_002203"
     CDS             13124..13456
                     /locus_tag="ONT85_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HCR2379159.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            13768..14259
                     /gene="nrdR"
                     /locus_tag="ONT85_002204"
     CDS             13768..14259
                     /gene="nrdR"
                     /locus_tag="ONT85_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HCR2379160.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            14282..15655
                     /locus_tag="ONT85_002205"
     CDS             14282..15655
                     /locus_tag="ONT85_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HCR2379161.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            15655..16581
                     /gene="dnaI"
                     /locus_tag="ONT85_002206"
     CDS             15655..16581
                     /gene="dnaI"
                     /locus_tag="ONT85_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HCR2379162.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            16710..17150
                     /locus_tag="ONT85_002207"
     CDS             16710..17150
                     /locus_tag="ONT85_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR2379163.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
BASE COUNT         5875 a         3008 c         3796 g         4546 t
ORIGIN      
        1 gatgtttacg aatgaaataa aaatcatgct ttatgtggac gatgtagaaa aaaatgcaac
       61 gttttggaga gcagttggct ttgcggaaaa agatcgtcaa gaaatggatg ggacgcttat
      121 aattgaagta ggagttgagg acagtcaaac ctcatttctg ttatatgatc gagcatttgt
      181 tgagtcccat tcacctggca cagctactag tgccccatct ttgatgtttt acagtaaaga
      241 tatctttgga ctatataaaa aaatgcagac gcaatcagtc acattaggtg atatggtaca
      301 gcttggagaa gaatatgtct ttaactttgc tgatcccgat ggcaactact ttgcggttac
      361 aggtaaataa tagaaataaa gactttgttt ttctgtgcga aacaggaaag cgaagtcttt
      421 ttaattcaaa taataatatt atgtaaacaa aaaactttcc atgcgtaatt atacacaagc
      481 aaataaatcg tcaaatgcga acgccttctt tatactgttt aggaacgaaa agaaggaagg
      541 aagaaataca tggattatca agtactgctt tattacaatt atacaaaaat cgaagaacca
      601 gaggaatttg ccaaaaatca tttagccttt tgcaaatctt tggatcttaa aggtcgtatt
      661 ctagtagctg aagaaggaat aaatggtact gtgtctggaa ccgttgaaaa cacgactgcc
      721 tatatgaaag caatgaaagc tgatcctcga tttgctgata cgatgttcaa gatcgatcca
      781 agcgaaggac atgcctttaa gaagatgttt gtaagatacc gtccagagct tgtaagttta
      841 aagctagaag aggacattga tccaaaccaa gtgacaggtg cctatctttc accgcaagaa
      901 tttaaagaag ctattttaga tgaacagaca gtggtgattg atgcacggaa tgattatgaa
      961 tatgacttgg gacattttcg aggggctgta cgcccagata tacgcagttt tcgagagctt
     1021 ccagactgga tccgcgagca caaagaagaa tttatggaaa aacgggtagt gacctactgt
     1081 actggtggca ttcgttgcga gaagttttct ggttggcttg tccgtgaagg attcaaagat
     1141 gtcggacagc tgcatggcgg tattgctact tatggtaagg atcctgaagt ccaaggagaa
     1201 ttatgggacg gcaagatgta tgtgtttgat gaacggatcg ctgtcgatat caaccaagta
     1261 gataaacaaa tcgtcggtcg cgactggttt gacggaacac catgtgaacg atatatcaat
     1321 tgtagcaatc ctgagtgcaa tcgtcagatt cttgcttcca ttgaaaatga agaaaaacat
     1381 ttaggagcat gtagcataga gtgtgcgaaa catccgcgca atcgctatgt tcaagaccat
     1441 ggaatctctc cagaagaagc agaactgcgg tttacagctt taattaaata gaaaacaata
     1501 gttttgattg tcgaccattt ccaatatgcg ctatactagc gttgaggatg gtgatccaca
     1561 tgaaatcatt gatttatgct attggggatg tccatggcaa acatgagctt tttgaaaaac
     1621 ttattcacta ttatgatcca aagatccatc agcttgtttt attaggggat ctcaatgatc
     1681 gaggtcctcg tacaaaagaa tgttttttat taggaaagca attagtcgaa gaaacaggag
     1741 ccatttacct ccggggaaac catgaagaat attttttgca atttctgcaa tcaccggagg
     1801 actggtatgc gccttatatg agaaatggtg gaaaagaaac gtttgaaagc ctgattcacc
     1861 caggggcatc ggaggaatac tccccaactg aaatctcgat gatgatccaa tcgagatatt
     1921 cagaactgat taaattttta gtccagcgcc ccttgtatta tgaatgggaa aattatcttt
     1981 ttgtccatgc cggtgtggat ttgacaaaga aagactggcg taagacaagt ctaagagact
     2041 ttatttggat aagggaacca tttcatgaag ggaaaaacaa tacaggtaag acaattgtat
     2101 ttggacatac gattacacca atgctccatg gagatatgca gacaacagac ctctggattc
     2161 aggatcataa aatcggaatg gacggtgggg ctgtattcgg agggtcattg catggggttg
     2221 tttttgacaa ggaaggtatt cttcaagata ttgaatatca gaacaacgaa ggaccttggc
     2281 atggaatctg actaatggaa agaatgaagg agctgtgact ttgttgcggc tctttttatt
     2341 tttatagaga atattgtttc tttcagtagt ttaacccagg ataaaaagta tgatagaata
     2401 aagaagcaat ttttagcaga aaagaggcat tttaatgacg gaaaatttga accagacgat
     2461 cattcagctt gtacaaaaag aactttcaga ctatcgtcct aagcagttga ctactgtact
     2521 taatttgtta aatgaaggaa acactgttcc ttttatcgcg cgttaccgaa aagaaatgac
     2581 tggaagtctt gatgaagtcc agattcgtga aatcgaagag cggtatgcct atcttgaaaa
     2641 cttagaaaaa cgtaaaactg aagtgattcg gctaatcgat gaacaaggga aactgactcc
     2701 cgagttagaa gcagaaatca cccaagccgt gaaaatgcaa caagtggaag atttgtatcg
     2761 tccatacaaa caaaaacgac ggacaaaagc aacgattgct aaagaaaaag gacttgaacc
     2821 actcgcgaag tggctgatgc agcttacaga tggagaggtg caatctgaag cagaaaaata
     2881 tattgacaaa gaaaaagaag tctcttctgc cgaagaagcg ttgcacggtg ctcatgagat
     2941 tattgctgaa caagtcagcg ataatgcaaa atttcggact tggattcggt cttatacgta
     3001 taacaaaggt atgtatgtca gcaatgtcaa ggatgagcaa gcagatgaaa aaggcgtata
     3061 tgaaatgtat tatgactttg cagaacccgt ccataaaatg gtttctcatc gaatattagc
     3121 tactaatcgt ggagaaaaag aagatattct gaaagtattc tttcaagtag atgaagcagc
     3181 aattttagct tacttggatc gccaaatagt caaaaatcct gcctctcctt cttcttcatt
     3241 cgtcagagaa gcctatcagg atagttataa acgctttatt cagccagcaa tcgagcgaga
     3301 actgcgcaat gaattgactg aaaaggcaga tgagcaagca atcgctattt tcggcgaaaa
     3361 cctccgtaat cttttgttgc aaccgccact aaaaggtaaa gttgtcctgg gatttgatcc
     3421 ggcttatcgt actggatgta aactagcagt cgtagatgcg acagggaaag ttttagcaat
     3481 cgaagttatt tatcctcata aacctgcagc gcaagcaaaa agagaagctg ctggaccagc
     3541 ttttatccaa ttaattaata aatatcaagt agatatggta gctatcggaa atggtacagc
     3601 cagtcgggaa tctgaacttt ttgtggctga gcagttgaaa tccgccgacc ataaaaccta
     3661 ttacgctatc gtcaacgaag caggcgcttc tgtttactcc gctagcgaaa ttgcacgaaa
     3721 agaatttccg catctacaag ttgaagaaag aagtgctgtt tctattgccc gtcgcctgca
     3781 agatccttta gctgaattag tcaaaatcga tcctaaagct gtcggagtag gccagtacca
     3841 acatgacgtt tctcaaaaac gtttggctga acaattggat tttgttgtgg aaacagcggt
     3901 taaccaagta ggtgtggatg tgaatacagc aagcccgcag ctcttgcagc atatatcagg
     3961 attgaataaa acaactgccc aaaacattgt catttatcgt gaagaaaacg gagaattcac
     4021 cgcacgaact caattgaaaa aagtgccacg cttaggacca aaagcatatg aacaagcaat
     4081 cggattcttg cgagtccctg gtgggaaaaa cattttggac aacacaggta tccatcccga
     4141 aagttattct attgcaaaag aaatattaac ttcagtccaa ctttcagaaa aagagcttgg
     4201 tacagaagaa gcagtagaaa aattgacacg attatccgta gagaaactgg ctgaaagcct
     4261 atcagtcgga gaagaaacgt tagccgatat tttagctggg ctgacacaac ctggacgaga
     4321 tatgcgtgat gagatgccag cgcccttatt acgtacagat gtattgagta tggaagactt
     4381 gaaaccaggg atggaattga ctggaacggt ccgtaatgtc attgacttcg gcgcatttgt
     4441 tgatatcggt gtgaagcaag acggacttgt ccatatatcg aaactcagca aaaaattcgt
     4501 caagcatcct acagatgtcg tgtcagtagg agatatcgta acagtttgga tagaacaagt
     4561 cgatacgaaa aaaggtagaa tcagtttgac gatgctttcc ccttatgaag agtaacaatg
     4621 aggagtaaca aaaattgaca caagaagaac tgcagcagtt agtagaagag atatcattaa
     4681 aaagcttcca gcttcctttc aagcaccaag caatgttcaa tccacgctta cgtacaactg
     4741 gcgggcgcta tcatttaaac gatcatcatt tagattttaa tgcgaagcta tttgaagagt
     4801 cagacgaaga gacgattgca ggaatcatca aacatgaact ttgccattat catcttcatc
     4861 tagctggaag agggtatcag cataaagata tggatttcaa gtatcttttg aaaaaaacag
     4921 gagggttgcg ctatgcacca gccttgacga agaaacagat aaagatggag tattatcgat
     4981 gccaagattg ttctgccacg atctgccgca agagaagaat cgatgtacgc aaatatcgtt
     5041 gtggaagatg tcatggagaa atcaaatgga ttgagacaaa aagagaagac tttaatgaag
     5101 cacaaaaaat cagatgaaag gatagaaggc ctttggaaaa aagtgagcag aggaatctaa
     5161 aagactggaa tcagctatta aattttttag ccgcacctga agagggaaat tttgaggaac
     5221 tgacggcacc tacacttatt ttagctggaa actgtcttcc tatcttagct gataaagcag
     5281 cacaaatgta tttaaatggc caagtagatc aattgttttt ggtcggaggg gtggggcatg
     5341 cgactagaat cctttatgag aattttgaaa agcaaggatt tcattttgaa gatggcatga
     5401 gtgaaagtga aatctgtcgt cagtatctaa aagaagtcta tgatttgccc gataaggctt
     5461 ttctgataga atcgaagtct acaaattctg gagagaacgc cactttttca ctggagattc
     5521 ttcattcctt aggagctgta ccagaaaaaa tcttattgat gaatgatccg acactgcaac
     5581 gcaggactcg agcaactttt gagaaagtat ggcaaaatga acaaactatt ttcgcgaatg
     5641 ctgtgccatt tgtgccggaa atccttcact tttctgaaga gatcatcttt acagcaaaag
     5701 aactgaatca tcaatggcct aaggaatatt tccatgcgct agtattagga gaaatggaac
     5761 gtctccatga cgatgagaac ggctatggtc cgaaaggaaa agattttatt cctcacattg
     5821 atatatcaga ggaagtttgg tcttcttaca ccagaattaa agagtcaata aaaacggatt
     5881 tttctcgtac ttgaccattt tagcaagcga atcctccttt ccaaataaat ttggttgaaa
     5941 ttttcaatcg tttataagat aatgaggagc gtgttgaatg taatagctaa aagaatggac
     6001 gctagataag agaagaggat tcaaatggaa aatcaaaata aattgtatca ttttgttggt
     6061 atcaaaggtt caggaatgag ctccttggct cttgttttac atgaacaagg tctaaatgtc
     6121 caaggatcag acattgaaaa atatttcttt acacaaagag atttagaaaa agcaaatatc
     6181 acgattctgc catttaacgc agataatgta aaaccaggga tgacgatcat tgcaggaaat
     6241 gcatttcctg attcacatga agaaatccag cgagcaaaag aattaggttt ggaagtgatt
     6301 cgctatcacg actttattgg tcattttatc caaaattata cgagtatcgc tgtaacaggc
     6361 tctcacggaa aaacaagtac gactggactg ctctctcatg tattgagtgg tgtccgtcca
     6421 acaagttatc taattggaga tggaacagga catggtgatc cgcaagcgga attcttttca
     6481 tttgaggcct gtgaatatcg ccgtcatttc cttgcctatt caccagacta tgcaatcatg
     6541 accaatatcg attttgatca tccggattat tatacaagta tcgacgatgt atttacagct
     6601 ttccaaacaa tggctggaca agtgaaaaaa gctatttttg cgtatggtga cgatgcttat
     6661 ttacgcaaat taaaagcaaa tgtgccgatt tattattatg gtgtcacaga aaacgatgat
     6721 atccaagcac gaaacatcga acgcacaact tctggttctg cttttgatgt ctatcatggg
     6781 gatgaatttg ttgggcactt cactgtacca gcattcggta aacacaatat cttaaatgct
     6841 ttaggtgtta ttgcagtagc ttatttcgaa aaactggatc tgaaagaagt tgcagaagaa
     6901 atgctgactt tcccaggagt aaaacgtcga ttcagtgaaa agatcgtagc agacatgacc
     6961 gtagtcgatg attatgcgca tcaccctgca gagatcaaag caacgattga tggcgcacgc
     7021 caaaaatatc cagataaaga aataattgca gttttccaac cccatacgtt tacgcgtacg
     7081 attgcattga tggatgaatt tgcagaagca cttgatttag ctgataaagt ttacttatgc
     7141 gatatttttg gttcggctcg ggaagaacaa ggaaacgtta agattgaaga ccttggagcg
     7201 aaaatcaaaa aaggtggaga agtcatcaaa gaaaataatg tatctccatt gttagattat
     7261 catgatgcag tagtaatctt catgggtgct ggagatgttc aaaaatttga acaagcgtat
     7321 gaaaaacttt taagcagcac gacgaaaaat gtactgtaat acaattactg cagtgtcctt
     7381 ataaaaatag aaagaggggg agtggcaaaa atcttgccaa gccccctttt ttgcaactta
     7441 tgataaacgg tgttcaaaag cttgcatctg cggtaataag cccaatcaaa tcaaaaacat
     7501 gagaagctgt tttttcttgg ctgttcttat tatttgatgc aggacagctt tttcacgacc
     7561 tctgtaaact ataggaatct gttaaatcaa gggagcagaa cttgcctttt acgctacttt
     7621 tggtacaata atgtatgatt aaaaataaac tggagggttt ctatcttgaa cataggtaag
     7681 ccgagaaaac taggaaaaac aataaatgaa atcgaggaag gcgactcgtt atccttaaca
     7741 gaatctattg aagataacca gcttttgctg tatttgggat tgacgaatga tgcaaatcct
     7801 ttgtatatcc aacatgactt tgcacaaaaa acagaatatc aacagccaat cgttccatca
     7861 gtgatgctga tggggatcat cacaagtgca atttcaaagc atcttcctgg accaggatca
     7921 catgttgtca atttttctgt caattttatt gaaccggttt tccattatga aacactgact
     7981 tttcagtttg aggtcataaa agtagataag atgaaggatg tggtgacgat ttctgtagaa
     8041 gcggagaata cgaacgaaga tcgtgtgttg gatgctgtcg ttatggtcca accgccacaa
     8101 gttcctgaat tggaaatagc aatcgaaagt gaaggtgaat tgaatgaata atccaagtac
     8161 atcaatgtca ggtatcaatc gtttttatgc aaaagtttat ggatttttag gtatgggact
     8221 tgcccttagt gcagtcactt cttacttggc tctttgggta tttcctttgc aattagcaac
     8281 ttttatcaat aattttccac taggctttgt gggtctgtgg atcgttgaac tgattttagt
     8341 aatggttcta ggcgtaaaag cacaaaagaa tccgagctta gcaatcggtg gatttattgc
     8401 ctactctctt ttgaatggca taacattagc ggttactctt gcatactacg atattggaac
     8461 agtaacgcaa gctttcttga cagctacttt gatgttcatg gggatgtctg ttgtcggagt
     8521 aacaacaaaa agagatctta gcggggttgg aagagcagga caggcagctt taatcggagt
     8581 gatcattgca ctagtcttga acatcttcat tttgagaagt agcgtagttg agctgttcat
     8641 ctccctactg ttagtcgtca tctttgcagg gattacagct tatgataacc aaagcatccg
     8701 taatatctat gtccagacaa acggaacagc acatagtgga attgctgtat tcatggcatt
     8761 gaagctgtat ttggacttta tcaacttgtt cttggcgttt ctgagaatct ttggacggaa
     8821 caactaaaaa agaaaaaaat tcatcagttg gggctatacc gaattatttg gtatggtccc
     8881 gctttttctt ttcgcattcc gcaggattct ttgttagaat gaataagaac tttacagtaa
     8941 ggagttaaga agatgacaaa aaataaatta ttactcgttg atgggaacag tgtggctttt
     9001 cgcgcattct ttgcgcttca caattctctt gaacgtttca aaaatagaaa cggtcttcat
     9061 acaaatgcaa tctatgcatt caatacgatg tttgaaaatg ttatgcaaaa agagcaaccg
     9121 actcatgttc tggttgcgtt cgatgcagga aaaacgactt ttcggacaga aatgtatgcg
     9181 gagtacaaag gtggaagatc caaaacacct ggcgaattca aagaacaaat gccatatata
     9241 cgtgatttgt tgacaggact aggggtacag tattacgaat tgcccaatta tgaagcagat
     9301 gatattatcg gtactttggc agaaaaagta gacaaagatc aatttgatgt cgtcgtgctt
     9361 tcaggtgatc gggatttgac tcaattagct agtaaagaag taaaagtgga tatcactgta
     9421 aaaggtgtca gcgatatcga aagctataca ccagaacatg tagcggaaaa atatgatggg
     9481 ttgacgccaa aacagattat tgacatgaag ggactagccg gagatgcctc tgacaatatc
     9541 cccggtgtga ctaaaatcgg tgaaaaaaca gctatcaagc tattgaaaca atatggctca
     9601 gtcgaaggaa tttatgaaca tatcgatgaa atgaagcaaa gcaaaatgaa agaaaatctg
     9661 ataaatgaca aagagcaggc tttcttatcg aaaaaattgg cgaccatcaa tacttcagcg
     9721 cctgtggatg tctctatcga ctcattgaag tatgaaggga aaaatctcga taaactcgtt
     9781 ccgttttata aagaaatgga tttcaatcag ttcctttcta aattgaatat cgtagaagaa
     9841 gaagtaaaaa tggatgatat tttgtttgaa gtcgttcatg aattcaaaga ggaaatgttt
     9901 actacggata tggcgttgta cgtggaaatg atgggcgaca attatcatac agaggaaatc
     9961 gtcggagtag cgtgggggac agaaaagaaa atttatgtca cgaacgatct atctgtattc
    10021 gaaaaccgag cgttccattc ttggatcact gatccgactc ggctgaaaaa agtatatgat
    10081 gcgaaacgaa cttatgttgc tttaaaccgc tatactggaa aaacgaaagg aatcgatttt
    10141 gatgtcttgc taggtgcata ccttctcgat acgaatgaaa aaagcacaga tatcgaagga
    10201 atagctgctc attacggata caacgatatc caatcagatg aagctgtata cggcaaaggg
    10261 gcgaaaaaag gtttaccaga agaagaggaa ctcttttttg ctcatttagc gcgaaaagta
    10321 gctgcaatca atgcgttgac accaaaattg agccaagaac tggcagaaaa aaaccaagaa
    10381 gatcttttcc ggaaaatgga actgccattg tctatcattt tggcagagat ggaaataagc
    10441 gggatcaaag tagatgcgac tcgcttacaa gaaatgaagg gcgagttctc tgctcgctta
    10501 agagagatcg aacaaaaaat ctacgaagaa gccggcgaag aattcaattt gaattcacca
    10561 aagcaattag gagtgatctt atttgaaaaa atgggattac ccgttatcaa aaaaacaaaa
    10621 actggatatt ccacagccgt agatgtactg gaacagcttc gtgaacaagc accaatcgta
    10681 gaggatatcc tcacctatcg tcaaattgca aaaattcaat ctacctatgt cgagggactg
    10741 ctgaaagtga tccaatcaga tggaaaagtg catacccgtt atgtccagac attgacccaa
    10801 acaggacgat taagctctgt tgatcctaat ttgcagaaca tcccgattcg attagatgaa
    10861 gggagaaaga tacgtcaggc atttgttcct agagaaaaag actggctgat ttattcttct
    10921 gactattcac aaatcgagtt acgcgtactt gcgcatatct caaatgatga acatttgaaa
    10981 gctgccttcc tagaaggtca agatatccat gccagcacag cgatgcgtgt atttggaatc
    11041 gaaaaggcag aggatgtcac accaaatatg cgtaggcagg caaaagcagt gaattttggt
    11101 atcgtgtatg gcatctctga ttacggactt tcacaaaatc taggaatcag ccgaaaagca
    11161 gcacagcaat acatcgatac gtactttgaa aaatacccag gtgtcaaaga atatatggaa
    11221 agaatcgtac gcgaagcaaa agaccaaggc tacgtcgaaa cgctgtatca ccgccgtcga
    11281 tatctgccag acatcaattc aagaaattac aatatccgtt cttttgcgga acgaacagct
    11341 atcaatacac caatccaagg aagtgctgct gatattttga aaatcgcgat gatcgaattg
    11401 gataaacgat taaaagaaac aggtcttcaa gctacgatgc tgttacaagt tcatgatgaa
    11461 cttgtcttcg aggtaccgga aaaagaactt gaatcattag ataaattggt gaaggaagtt
    11521 atggaacaag ctgtttctct tcatgtacca ctgatcactg acagcagttg gggaaaaacg
    11581 tggtacgaag caaaataaac attgtttaag acagagcaat ataatgaagg tgaaaagaaa
    11641 aaatgccaga attaccagaa gtagaaaccg ttcgtaaagg attggaacgg ttagtagtag
    11701 gaaaaacgat tcaaaaagtt caggtcttgt ggcccaagat catcgagcaa ccagagacac
    11761 ccatctttga ggcttctctt gttggcgaaa ccatccaatc cattggacgt agagggaagt
    11821 ttttaatttt tcatctcgat cattgtgaac tgatctcaca cttgcgaatg gaaggaaaat
    11881 atcaatttac gaaagaaaat acaccaatcg ataaacatac gcatgtactt ttcttttttg
    11941 aggatggaag ccaacttcgc tacaacgatg tacgaaaatt tggtcgtatg acaatcgtcg
    12001 aaaaaggagc aagtgcaact taccgaggaa tcatgaaatt gggacctgag cctttgccag
    12061 atagcttctt attagcagat ttcgcaaatg gactgaaaaa gtcccacaaa gcaatcaagc
    12121 cattgctgct agaccaacgt ctagtgactg gtctaggtaa tatttatgta gatgaagcat
    12181 tatgggaagc gaagatccat ccagaacaac cggcgaacac actgcgcccc aaagaggtag
    12241 aacaattacg tctatcgatc attgatgttt tggatcgtgc aatagaagcc ggaggtacca
    12301 cgattcgcag ttatttgaat gcattaggag aatcaggcgg atttcaagta gcactgcatg
    12361 tctatcagca aactggcaaa ccatgtatac gttgtggaac gccgatcgtg aaaacaaaag
    12421 tggcacaaag ggggacgcac tattgtccga attgtcaaag gctaaaaatc gcaaagtagg
    12481 atttgtttta ggtttgacgg gtggaatagc taccggtaaa agtacggcag ccaaagtctt
    12541 tcagtctcat ggatttcctt tgatcgatgg cgatgtgatt gccagagaaa ctgttgagcc
    12601 tggaacgctt gctctgagga aaattgtatc agtgtttggc caagaggtat tgcagcctga
    12661 tgggcaacta gatcgaggga aacttgggat gattgttttt ccatcaaagg aaaaacggca
    12721 aaaacttgat cagctattag atccgtttat ccgaaaagcg ataaaagatc aaatcgcctc
    12781 tctaagttcc aatcatcctt tagtaattgt agatatccca ttgctctacg aaggtcatta
    12841 tgaccactat atggatgcag tagctgtcgt ttatactacg ccagaaactc agcttcagcg
    12901 tttgatgaaa cgtaatcaac tgacgctaga acaagcgcag cagagaatta gtagtcaact
    12961 tccaatcgaa gaaaaaaagc agcgtgcgga tattttgttc gacaacaatg gaacaaaaga
    13021 aaaacttgtg ggacaaatcg aaaattggct ttttgctaat cgctttcttt gagtatctgt
    13081 gtcaattttg ctatcctgag tatatagaaa gtgagggata gcgatgggat cattaaaaaa
    13141 tcaaaaggat aaagtcgtag gtaacgcaaa agaaaaaatc ggcgagatgc tcaataaaga
    13201 aaatctaatc gatgaaggtc gtacgcaatc agaaacagca gaagaaaaag aaaagcagta
    13261 ccaagcaaga atcgatgaaa tagaagacca gcaggcagaa gaagaagcga agcggcaaga
    13321 tttgactcaa gaacgaaaaa atccgacacc tgatcgtagt aaaacaccgc tcccagaaag
    13381 tgaccacatt cccgatgagc aaaaaatcga ggaacagcga atgaaacact atacaggaat
    13441 cgaagagaag ttataaaata gtaaaaaatc gagatgtgac ttgaagcaga tgagaaggtg
    13501 ttcatctgct tcaagttttt tgtgctagat gactaaatca gcttttgtgt aagttatttc
    13561 tcagtaatca aggtgatcag tattggaaaa ttgaaattta gtaaggacag gggacacaat
    13621 tacgcatgaa agtagaaaaa attgttctta tatccgtgtt ttttgggtag gacagtcgcg
    13681 tttgtgattt gtttcgtgtt ataatacaac tagttgtaaa gaaagacaca tcctttatat
    13741 aaaatgcgat gcaagaggtg agacaaaatg agatgtccca gatgtcaaca taataattca
    13801 cgagtaatcg acagccgtca agcagatgat ggacgtgcga tccgtcgccg tcgcgaatgt
    13861 gagaattgtt cgttccgatt cactacattt gaacgaattg aagctgctcc gttattagta
    13921 atcaagaaaa acggggaacg cgaagagttc aatcgcgata aaattttaag aggactcatc
    13981 cgttcagcag aaaaaagacc tgtagctatg gaacaaatgg aacaaatcgt caaccatgtc
    14041 gaaaaccgcg tacgcagtct cggagaaaac gaagtatcga cgaatctaat tggtgaatat
    14101 gtgatggaag atttagttga tttagacgaa atcgcttata ttcgttttgc aagtgtttat
    14161 cgccagttca aagatatgtc tgttttctta aaagagttgc aagatatcgt ggataaagca
    14221 aaagaaaata ataaagagaa taataaagaa aacaattaag aaacaaaaag gaggcatttc
    14281 cttggaaaat gcttggaaag agcttcaacc aaaaaatatt taccaaggaa gaaaagcggc
    14341 tccgctgaga gaagacagca agaacgcctt gctgtatctt tatcagccac taatcggcgg
    14401 agaagccttg agtatctatt tcaccctgtt atcagaaatc tctcaagaat ctggcaaagg
    14461 tcctgaaggg atgcatgcag atctgcttag tagtctaggc tgcggtatcc cgcaatttta
    14521 tgatgcaaga aaaaagctag aaggaatcgg cttactggat gtttatttta aagaagatag
    14581 tgatctggga cctcgtttta tttatgagct gttagaacca ctcattccta aagcattttt
    14641 caaagactct gttctggctt ttcttcttct ggaaaaagtt ggtgaacgac gattccagaa
    14701 gcttacccaa ttctttgaac ctaaaaggca tacagcagag ggatatcaaa aaatcacaaa
    14761 aaaattttta gatgtttatc gcttcaatga agcagtgtac gctgctaatc aagaaaacat
    14821 cgaatcgctg aataaatctt ttgaagaatc tgaatcagta gcattacttg aagcagattc
    14881 gacattcgat tggcagtttt tgaatgattg gctggcaaaa aaacatattg caagtcttga
    14941 tgatccaatc atcaaacaag tcgacatgta tcatcagctt tacggtttcg atgaattgac
    15001 acttggcgaa ctgatcagtc aatcttatga ctttagtgaa caaaaagtat ctgaaaaaga
    15061 actgcaaaaa gccattttgg caatgcagag acgtccggtg aagcaaacaa aagaaacagt
    15121 tgcggtttct gaaaacagta atgaaccctc gtcaaacgaa catttgacaa acgcagcgct
    15181 tcagctgata aaagaagcac agagcattcc accgatgaaa tatctggaag caatcaagtc
    15241 agaaaaaggc ggatatgttt ctaaacaaga aaactggtta ctgcaagatc ttgtttctca
    15301 atcaggtctt tccagtagcg tgatcaatgt tttgctgaat tatgtactag tgatcaagaa
    15361 taatgctagc ttaaatgcca gttatgtaaa tacagtggca aatgaatggg cacaaaagaa
    15421 aatcgccaca gcagaagaag cgatccagca cattcggcaa ataagtaatc aagcaaaaca
    15481 atcaaaacaa aagaagcaaa caaattatca aactggcaag cgaaatgttc gacgggaaaa
    15541 gcttcctgat tgggtcaacc agccaaaaga tgaaaaacag atcagcagcg agaaaaaagc
    15601 agagattgac cgccgtttta aagaatacct ggcaaaaaag gagggtgata tctagtggaa
    15661 gacgtaggca aagaattgaa gaggatcatc agcaaacgca attatcaaga aaagtatgct
    15721 gaaatgatcg ctgaagtctt aaatgatgcg gatgttcaag cttttctaag cgcacaccaa
    15781 gaagaattga cacaagcgga tattgaaaaa agttacgcta aattgtatga attcgtgcaa
    15841 gaaaagcgta aatatcaaaa aaacgatcca acgatgatcg ctcccggcta tgagccgaag
    15901 ctgacgttga attttcatta tgtggatgtc acttatgtac caacggaagc attgttagca
    15961 cggcaaagac aagaagaaat cagaagccga gtaaaagcaa tggatatgcc aaaagatatt
    16021 caatcggctt cttttgaaaa ctttgaacgt acagatggac gaggatatgc gagcttagaa
    16081 gccctagatt ttgtagagaa atatactgct gatccaaaag ggtatcataa aggactgtat
    16141 ctagtaggaa gttttggtat tggaaaaaca tatttattag gagcaacagc gagagaatta
    16201 gcgattgctg gattcaccac gacgctagtc cattttccga catttgccgt tgagatgaaa
    16261 caggcaatcg gaaaagatca agtggcagaa aagttagatg cagtgaaacg ttcacctatt
    16321 ttgatgatcg atgacattgg tgcagatgcg atgagtagtt ggatacgaga cgaagtattc
    16381 ggtgttatcc ttcaatatcg gatgcaggaa cagttaccaa ccttcttctc atcgaatttt
    16441 acaatggatg aattggaaca gcatttgtcc gtcactcaac gtggagatga agagccgcta
    16501 aaagcgaaac ggataatgga acgcatacgt tatttgacaa aagaaatcga aatgactggt
    16561 cgcaaccgac gcaatggttg aacagttgac aagtccaact gacatttgac ctagtagtaa
    16621 aagcaggtca gcgaatcatt atatggacag aaatgaccgg agacaatcac cgcaacaagt
    16681 aatgcagaaa cttaaacgga ggatttttta tgagggctat tttttctagt atatctgtag
    16741 ttggtctatt ttttctttta gcaggctgcg gtagcgcaga tagcaacaca ggttctgttg
    16801 attcgattga tgcgggtcaa acagtaaaaa caagcagtac gaaaacggag agacaaagca
    16861 tatttgtcgg aacgattgaa agtattactc aagtagaagc agaagaagaa acgcttcagt
    16921 tgaaactcgt cgatgtcatg gccaaagaag atccagcgaa tatgggaacg tcattcagca
    16981 atgacggagt gatcttaaat gctactgctg aaaaaataag cgtggacata aatgaattga
    17041 aaatcggtga caaagtccaa tttacattag ttgagatgcc gatcatgacg atgtctatcc
    17101 caccgcaagt tcctggagat tcgattattg atgtttcatt gaacaaataa ccaataacat
    17161 tttttcaaaa ataagaaaat gttgttttat agaacgaaaa ggtatatttt ttaaggagat
    17221 atggg
//
LOCUS       DAJSBL010000056        17152 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.058, whole genome shotgun sequence.
ACCESSION   DAJSBL010000056 DAJSBL010000000
VERSION     DAJSBL010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17152
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.058"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            138..1475
                     /locus_tag="ONT85_002208"
     CDS             138..1475
                     /locus_tag="ONT85_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HCR2379164.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAILCLITFLLYGNA"
     gene            1523..3652
                     /locus_tag="ONT85_002209"
     CDS             1523..3652
                     /locus_tag="ONT85_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HCR2379165.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLLENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            3627..4316
                     /locus_tag="ONT85_002210"
     CDS             3627..4316
                     /locus_tag="ONT85_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2379166.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
     gene            complement(4724..5410)
                     /locus_tag="ONT85_002211"
                     /pseudo
     CDS             complement(4724..5410)
                     /locus_tag="ONT85_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
     gene            complement(5546..5713)
                     /locus_tag="ONT85_002212"
     CDS             complement(5546..5713)
                     /locus_tag="ONT85_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2379167.1"
                     /translation="MYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKESEAFAKRIGG
                     RVKPIQEVRPD"
     gene            complement(5792..6232)
                     /locus_tag="ONT85_002213"
     CDS             complement(5792..6232)
                     /locus_tag="ONT85_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HCR2379168.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            complement(6236..7081)
                     /locus_tag="ONT85_002214"
     CDS             complement(6236..7081)
                     /locus_tag="ONT85_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HCR2379169.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVVWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLAANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAIYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            7230..8648
                     /gene="pepV"
                     /locus_tag="ONT85_002215"
     CDS             7230..8648
                     /gene="pepV"
                     /locus_tag="ONT85_002215"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HCR2379170.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            complement(8705..9652)
                     /locus_tag="ONT85_002216"
     CDS             complement(8705..9652)
                     /locus_tag="ONT85_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HCR2379171.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
     gene            complement(9875..10225)
                     /locus_tag="ONT85_002217"
     CDS             complement(9875..10225)
                     /locus_tag="ONT85_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HCR2379172.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
     gene            10425..11504
                     /gene="pepA"
                     /locus_tag="ONT85_002218"
     CDS             10425..11504
                     /gene="pepA"
                     /locus_tag="ONT85_002218"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HCR2379173.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            11648..11968
                     /locus_tag="ONT85_002219"
     CDS             11648..11968
                     /locus_tag="ONT85_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HCR2379174.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            11990..12454
                     /locus_tag="ONT85_002220"
     CDS             11990..12454
                     /locus_tag="ONT85_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HCR2379175.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            12659..13264
                     /locus_tag="ONT85_002221"
     CDS             12659..13264
                     /locus_tag="ONT85_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HCR2379176.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
     gene            complement(13303..14592)
                     /locus_tag="ONT85_002222"
     CDS             complement(13303..14592)
                     /locus_tag="ONT85_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HCR2379177.1"
                     /translation="MDRKNVTPKMKKSKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            15020..15499
                     /gene="rlmH"
                     /locus_tag="ONT85_002223"
     CDS             15020..15499
                     /gene="rlmH"
                     /locus_tag="ONT85_002223"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HCR2379178.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYALAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            15702..16252
                     /locus_tag="ONT85_002224"
                     /pseudo
     CDS             15702..16252
                     /locus_tag="ONT85_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299296.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
     gene            16323..>17152
                     /locus_tag="ONT85_002225"
     CDS             16323..>17152
                     /locus_tag="ONT85_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEfa13 family transposase"
                     /protein_id="HCR2379179.1"
                     /translation="MNDFTTEIMEILINKGDLDDLFRRHLELAINALLQAELTVFLDY
                     EKYDRAGFNSGNSRNGNYSRSFKTEYGELNLVIPRDRNGDFSQQTLPAYKRTNDSLET
                     TIIQLVQKGITMSEISDLIEKMYGHYYKPQTISNMSKIVSEDVSAFKERTLEAKYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFTIAPTESAYVWKEILQDLKDRG
                     LEEVLLVVTDGLSGIDDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLV
                     "
BASE COUNT         5460 a         3105 c         3467 g         5120 t
ORIGIN      
        1 cataattaag gttttttaat tgaaaactga acatttatgg tcacccttta atgaaagcgc
       61 taactacata agttagaatg agtttgactg atatattagt ttgcttatca gaaaaacaaa
      121 atggacggag gaataacatg agtgatatta ttattggtat tttgttagtt atctcattct
      181 ttttcatggt ctggtactgt gtaaaaggct acaacttgat ggtcggcttt gccatcatgg
      241 ctactgtatg gatgggatta gcgctagtcg gaaacacgtt ttcaccaaac ccggcgatgg
      301 aagggcaagg agtaattgat gtgttgacgc atatctatac gacggggcca gctgagtacg
      361 ccaaatcgat cttggtaaat gtcttctttg gtgccttttt cgggcgtgtt ctagtagata
      421 gcggaatcgc agctaccctg attcgaaagg tagttgaact tggaggggac aagccaagaa
      481 tcacgatgag cttactatgt attgtaaccg ctgttatatt catgtcgatg acaggaatcg
      541 gaccagtgat ttctattgca gttattgtgt tgccaatctt gatgtcatta ggtatttcag
      601 taccggttgc actgttctca tttatgggct cgattatggc tggaatcttt gctaatatcg
      661 tcaatttcaa acaatatcaa acgatttatg ctggtttcaa tccagcggct gaaagctata
      721 cttataatga ctatttccaa attggtatga ttgggatgat tgtaagctta gttgtggtac
      781 taacggtcgc taatatatca atgaacaaaa agaaacgcta tgcaatggca gcaaacgtac
      841 cagctgaagg tggcgacgca ccaatgatct catggttagc cgtattatta ccagttttag
      901 gggtcgttct tttagatctt ccaatcatct taggttttat tctagcaggt atctgggcat
      961 tgctgtttac tggaaaattg cgcggaggtt ataaagaaat ctgtcgtcag tttgcgaaat
     1021 tatttacaga tggcgcagta gatgttgcgc caatggttgg tttcttgatg accttagcga
     1081 tgttcaataa ttcagctgcg tatgcttctc cttatttttc agctattttt ggagattgga
     1141 taccgcaatc accattagtc ttggcaatcg tatttgctat attgacacca ctaggatttt
     1201 tccgcggtcc aatgaatcta gtcggttcag gctcagctat tttagctgtc gtattagctg
     1261 ttaatccaac gatgtcgcca gcctttttgt tcccgttatt tgcaatcaca acgattgctc
     1321 ctcagcattt agatattaca caatcttggg ttgcttgggg attaggttat acaaaagtaa
     1381 cttctcgaga atatatgaag aaatccatcc caactggttg gatcatcgga gcgatcttat
     1441 gcctgatcac gttcttattg tacgggaacg cctagtaaac aaaacgtcga taaaaacgat
     1501 tagatgaatg gaggaaaaga aaatgaaacg ttttgtgcga atgggaattg atgttggcgg
     1561 tacacatacg aaagcagtcg caatcgataa tgcgacgcac gaaatcatcg gaaaatcttc
     1621 agtgaaaaca acccacgatg atgtccgtgg agtagccgca ggtgtcgttc agtcttttca
     1681 aaattgtttg ctggaaaata atatcagtcc agaagatgtt gtgtttgttg cacattctac
     1741 cacacaagcg actaatgcac tgattgaagg agacgtagca aaagtcggcg taatcgggat
     1801 ggcaaaaggt ggattagaag gttttttagc aaaacgacaa actagattga acgatatcga
     1861 tttagggaat aagaaaaaaa tagagattgt caatgcattc ttacctgtca aacacttaaa
     1921 tgtagaccgt gtttcagaaa cgatttcttc tttagaaaga gaaagagcgg aggtcttggt
     1981 ttcttctatg gcatttggag tagataatgg ggaaccagaa cgagttgttt atgaagcagc
     2041 atcggtaaaa tcgatcccta caacgatggc ttctgacatc acaaaattat atggattgac
     2101 aagaagaact cggacagcag caatcaatgc atctatttta ccaaaaatgc tagatacggc
     2161 aacctctaca gaagattccg tacgagaagc aggggtcaat gtttctttga tgattatgcg
     2221 cggagacggc ggagtaatgg aaatcaatga aatgaaaaaa cgacctgttt taaccatgct
     2281 ttccggtcca gcagcatcag tcatgggctc tttgatgtac ttgagagcgt ctaatggtgt
     2341 ttattttgaa gtaggcggaa caacaaccaa tatcggggtc attaaaaatg gacgtccagc
     2401 aatcgattat tcaattgtag gtggccaccc aacatatatc tcttcattag atgttcgggt
     2461 tctaggtgtg gcaggcggtt cgatggtacg ggctaatcag tcaggaatca tcgatgtcgg
     2521 tccacgttct gcacatattg ccggtctaga ttatgcagta tttacggaga cagaaaaaat
     2581 caaaggtcct aaggttgaat ttttctctcc taaagaagga gaccctgctg attacgtcaa
     2641 ggtagtgatg gaagacggag aagaagtgac tattacaaat acatgtgcag ccaatgtcct
     2701 tggattagtg caagaagaac atttttctta tggaaatgtc ccatctgcac gaaaagccat
     2761 ccaagcatta gctgattatt gtcatacgac ggttgaagac atcgcagaac agatcatgga
     2821 aaaatcctat gctaagattg aaccggtgat tttagaattg gcagacaagt atcacttgga
     2881 gaaagatcag atttctcttg taggtgtagg gggcggcgct gcctcattga ttacttactt
     2941 tagtaataaa atgggtgtga aatattcgat tcctgaaaac gccgaagtga tttcttctat
     3001 tggtgtagca cttgcaatgg tccgagatgt tgtagaacgt atcatccctt cgccaagtaa
     3061 agaagatata cgatcgttga aaaatgaagc gatgaataaa gcgattgaat caggcgctac
     3121 accagaatcg atcgaagtcc acgtagaaat cgatccacag acttcaaaag taacggctat
     3181 cgctactggt tctacggaag tgaaagcaac cgatctaacc aaggagatca caacagaaga
     3241 agcactagag cttgctgcag aggatatgcg attaaataaa aatgaagttt gcttattgga
     3301 aaatacacct ttcttttatg tatgcggcga acagaatcgt tcaaaaaatg ctggaagtct
     3361 tcggatcatc gatcaaaaag gatttatcaa ggtccaacgt ggacatgctt catgtatgaa
     3421 aacaactgca gccaattata tgacagctgt cgaacaactt tgggaagata tggcagttta
     3481 tcaaactgaa ttgattgccc gaccagaatt ttatctctgc ttaggagcaa gagtctctga
     3541 ttttacagcg acggatctgg aacaactaca gcttttgatg gatctggaag tatctactat
     3601 ggaaccggag gaagaagtga tcgtcgttgc cggaaatatc aaacaaacat aagttagaag
     3661 agttactaaa cgaattacaa cagatccccg aagaaatttg gggattctac cagtttcggc
     3721 gagatttatt ttggaaaaag attcctttgt ccaagcagaa aattcttata caacaatcaa
     3781 tcgattgtgg tatcgaaacg gcatgtagca tcaaaaaaaa gtaccctttt gccgatgtag
     3841 gagaaatttg tgaacaaatg gctgttccaa tcgtttcctg tgaatcagag caaataaacg
     3901 aacgaatcac ttttgcaact tatgcagaag atgaagggat tcgattgatg acggagccgt
     3961 tagaaaaatt aaaatgttca ggactagctt cgatctctaa agaaacagcg caagctttga
     4021 ttattggaca tgaactattt caccacatcg aagcttctgt taagggaatt tatacgcaaa
     4081 gcgaaaaaat tgtgctgtgg cggttgcctt tttatacaca tcaatcaaac attcgtgcat
     4141 tgagcgagat agcagcgatg agtttttcaa aagagatgaa tcaaagcgat ttttcgcctt
     4201 atgtattaga ggcagtctta ctttggccat ataatgaaat tcgtagccaa gggatcttag
     4261 aagaaataaa aaaaatagaa aaaagatgta cggagtatga tttagattac aaataaagag
     4321 actgtgacaa aagtcgtgtc acagtccttt ttttccgaat aaacggtgtt ctcctgtcaa
     4381 ctcctcggtc acaagtcaaa atattcaaca aacatgagaa gctgtttttt gaaattgttc
     4441 cttgtgactc ggagatgaac ggcaggctca cgacctcttg gaaaacggtg ttcaaaagct
     4501 tgcatctgcg gtaataagtc cagccaagtt aaaaacataa gaagctattt ttacttggct
     4561 gttcttatta cttgatgcag ggcagctttt tcacaacctc tcaataaacg gtgtgcaatc
     4621 agctgaccct cggtattagc tcaaaaaaaa cggcaaaata tgaggaacta ttttgccgtt
     4681 tctttctcta atactcaggt caaaacgctg attccaaaac ctcttatttg atgggtctgt
     4741 cctgaatatc ttcaaaataa tcggggtaat tatgaatcac gtattcttgc tcctcgataa
     4801 tcatatgtaa atgcatcgtg caaagaaatc cgacttcatc aaagttatga tcaatcattg
     4861 attgaagcat tgtctgatgt tcatctaata ctcgacccca gtctaattct gaaatcgtca
     4921 aacgtaacca tctgaaacgg ttcaagtgcg tactataact ttctagccaa tcccaaattt
     4981 ctcgttttcc agcaatttca taacaagttc ggtgaaaatc agtatccaaa tcaaaaaaag
     5041 cttttttatt ttgagcgatt actgcctgat cggtttgttc tagtattttt tctagtttag
     5101 cttgatctta cttggtcatt ttcgcactgc attcttgcag gatcggtttt tccaactttt
     5161 cccgcgtata acgggctaat gaagcagatc ggatatcgat ttttgaaaca taagtgccgg
     5221 actgtggaat ggtttttaca agctcttgat tgcgcagttg gatcagtgct tctcgaatcg
     5281 gtgtacgccc gatagctagt tgttcttcta acactttatc ggagatttta cttccaggtg
     5341 ctaactcgga ataaatgatt tgtttacgta tagattcata agcttgctgt tgcaaattat
     5401 tgataaccat ttaaccttcc tttctatgca gatataatct attataacat aaatcgctta
     5461 caaatcgtgt gggcataaaa acacagtctg caaaaaaagc cctgataggc atgcctatta
     5521 gagcctcgaa aagaaaaata ttgaatcaat ctggtcgtac ctcttggatc ggtttgacac
     5581 gaccgccgat tcttttagca aaagcttcgg attctttttt gtctttgaaa atgagcagat
     5641 tgtctaagtt tttttcccga gtgcgacagc cagttttatc gatgtaaccg tgcattactt
     5701 taacgacata catatgggga tcacctgctt ttttcttttc attatacgaa ctactcgaca
     5761 caatacaaga aaaatgctta gacggtcttt attaagaatt tacatcatcc aaagaagcgt
     5821 acgcttggaa taactgatct aactccggat tgactttgct cagcttcact aatcgttgcg
     5881 aatcagaaag taaaaaacag tgtttactac tgccagtagt ttttaatttt ttgtcttcag
     5941 ttcccacaat ttcgtagcta aaatccaacc gtgtccccgt atacttttgg atagttggat
     6001 ggatgctcac tttttctccg taatggatca tttctttata gctgcaattt acttccagaa
     6061 cggggatgat gatcccagat tcttcgattt ttttataggg aaaccctaga tgtaccaata
     6121 aggctacccg agcctcttca aaccagcgga tataattgct gtgatgaata atccccattt
     6181 gatctgtttc ataataaaaa ggttctcgca cataacctgt aaatttctcc atgactcatg
     6241 cctcgttttc cattgatttt ttacaaaaat aattcatcca tttcggaagt gcttcgctga
     6301 tttgtgtcgg caaaacaata tattgttctt ttgccagctg gtctccaatg aaactatgaa
     6361 gataaatagc tgctgcaatc gtttggtcat tttgaggaaa ctgggctaaa aatcctgtga
     6421 tcatcccagc taaagtgtca cccatgccgc ccgttgccat agcagcagaa cctaaaggat
     6481 tggtgtagaa gtgctggtga ccataaattt ttgtctcatg acttttcaaa acgagcacag
     6541 caccgatttc ctgttgcttc tttttgtttt ctttatcccg ttgttcttgt atcggtagag
     6601 aactcaaccg ttgccactcc atttgatgag gtgtaagtac agtttgttcg ggatagggta
     6661 gcaaaagatg attagcagca agtaaactta aagcagagcc atcaatcacc agccattgtt
     6721 cttttgattg gttttctagt acttgtttga aacgttccaa actcttagaa gaagtaccca
     6781 atccgggacc aatcaagagt acatctgctt gctttattac ggaaagaaac tggtcagtat
     6841 ccgtccaaac tacaatcatt gcttctggca gatgactgtg aagagcactc cgattttttt
     6901 catcagttac tacagtcgtg agacccgctc cgctgtgtac ggcagctaaa gcactcataa
     6961 tgattgctcc gccatattgt tcattgccgc caatcaaaac gatacgtcca aatgttcctt
     7021 tatgtgaaat ttttggacga ggtgtgatga ctcttttcac tagttgttcg tttatcagca
     7081 tctaaaaacc tccttttact cattatagcg tatatttttt ttagagaaaa acagataaga
     7141 aaaggaattg tcagattttt tgaactattt ttttctgtaa tttgtggtat catgaacacg
     7201 aagacttaaa taagtggagg attaacttta tgacaattga ttggcaaaaa gaagtagaag
     7261 cacgtaaaga agacttgctt gaagacttga aaaatctttt acgtgtcaac agcgagcgcg
     7321 atgactcaaa agtaacacca gatgcaccat ttggacctgg acctcgcgac gcattgaaac
     7381 atatgttagc ttatggcgaa cgtgatggat ttaaagtaaa aaatgtcgat aactatgcag
     7441 ggcatattga tcttggcgaa ggagacgaaa cgctagggat atttggacac atggatgtcg
     7501 ttcctgcagg agatggctgg gacacagatc cttatgaacc agtaatcaag gatggaaaga
     7561 tctttgcacg cggatcaagc gatgacaaag gtccaagtat ggcagcttac tatgcgatga
     7621 agatcattaa agaacttgac ttgaaactat caaaaaaagt tcgcttcgta gtcggcagcg
     7681 atgaagaaag cggctggggc gatatggctt attatttcga acatgaagaa gaaccagatt
     7741 ttggtttctc accagatgct gaattcccaa tcatcaatgg tgaaaaagga aatgtttctt
     7801 tagctttacg cttcaaaggc gacaatgcag gagattatgt cttgaaatct tttgtttccg
     7861 gacttcgcga aaatatggtg ccaggaacag caactgcagc acttcaagta ccaagtgcag
     7921 atgcagctat cgcgatggaa gaagcatttt accaattcat cgaagcaaat ccagtaagcg
     7981 gaacgattga agcggacaat acgtatgtaa aaatcgaatt agttggtaaa ggcgcacatg
     8041 gtgcaagccc acaatctggt atcaatgcag gttctttctt ggcattgttc ttagataact
     8101 atgaattcct aggatctgct aaacaattta tccatgtagc agctgcttat gtccatgaag
     8161 atttttatgg agaaaaacta ggagtcgctt atgaagatga aaaaatgggc aaattgacaa
     8221 tgaatgctgg actatttgct tttgaagaaa atggtacaga ggaagcgaac ttcatcaata
     8281 tgaatttccg ttttccaaaa ggtgtaacag tagacggatt gcaaagtgac atcgaacaaa
     8341 cagtggggca agaaggcgca acagttactc gaggtgctcg cgtaatggaa ccacattacg
     8401 taccaatgga tgatccattg gtggctacat tattgcaagt ctatgaagac cacactggag
     8461 aaaaaggcta cgaacaaatc atcggtggcg gaacttacgg acgtttgttg aaacgtggcg
     8521 ttgcttatgg agcgatgttc ccaggatata ctgatacaat gcatcaagca aacgaattta
     8581 tgagtcttga tgacttgttc agagcaacag caatctatgc agatgccatc tatcgtttgg
     8641 cgaaataatt aattaaaaag aggatcaaga attgcaaatt atttttgcag tcttgatcct
     8701 ttttttactt tctactgtgg tattcatgat aaacatgata tagattcaat tcatattctt
     8761 tcttagcgtt cgtgacgact gtatccaaaa tcaaccctgt tactaatgaa agaagtgcta
     8821 aagtcatcaa tccagtactt aaaatcgcag aagggatgcg tgtaataaag cttgtcagca
     8881 tatattcgcg aatcacagga atccccgtga ccaatccaaa taaaaagaag aagatggtcc
     8941 aaatcccaaa gaaaagcaaa ggcttataat ctttaaacat tttaataatc atcatgatca
     9001 ctttgaatcc gtcagagaat gtattcaatt ttgattcgct gccttcagga cgatcacgat
     9061 aatcgattgg gatttcaacg aacttgaatt ttttatctaa agcatggatc gtcaattccg
     9121 tttcgatttg aaaaccagaa ctcataatag gaaaactttt gacaaagata cgattgaatc
     9181 ctcgatagcc ggtcatgaca tcaagaattt ttgttttata cattctagta attgtatttc
     9241 taacaaggtt atttccaaaa tcgtggaaag gtctcttatt ctcatcaaag taggttccat
     9301 ttgataggcg atcaccaatg accatatctg cttcaccaga acgtaatttc tcgagcaaac
     9361 catgtacagc ttctgcagga taagtatcat cgccatctac cattaaataa tagtcggcat
     9421 cgatatcaaa gaacatttgg cggatcacat ttccttttcc ttgacgaggt tcttttttta
     9481 cgattgctcc accttgaacg gccaattcgt atgttcgatc ggtcgaatta ttatcataga
     9541 cataaatatc tgcttctggt aattctcgtt taaagtctgc aatgactgtt gaaattgttg
     9601 cttcctcatt gtaacaagga atcagaacag caattttttc ttgttgttcc atttttcact
     9661 ctccttaact atagaaaaca aaagctaaat taaaaatacc tcataatcaa aggaaatgct
     9721 agtttttccc gctaaatatc atttatttaa aaagaaaata ctggaatata tgtttggaaa
     9781 gctctatcta aaaaaccacc cactacaaaa gaaggacatg atacctctct tttgaatagt
     9841 gggtgcgtat tcgttctctc acttttttga cgtattaatc tgttgcacct ttttttcttg
     9901 aaatttctaa tacggttcct gtatgagcat ctgctaaaaa ttcataataa acagtttcgc
     9961 catcttctat tcgagaaaga cctccccgat aagcactcga ccgaacggcg aatttcctaa
    10021 taggttcctt ttcaaaagag atccaagaac cttcgattgc accttctttt agaaaagatg
    10081 acttgatatt ttcaagtact tggtctgctg acaatacttt tttgcgatga taccaaatcg
    10141 ttgaagctac cccgctcaat aatcctagac cgattccaac tgctaacccg cctttaaaat
    10201 agcttagttc gctttcttca tacatgtgga catacctcct tcttctcact tgttactcct
    10261 tgtgcttatt ttaccacact ctcatatttt ctttgaaata aataaccgct accagaaaaa
    10321 ataagctata ataaatacga tcggtctgca gaaaaaatca tattttatcc gcagatcaat
    10381 ataagtctga gaggagttta gtctgaaatt tagacgaata gcatatggaa gaaaaaacat
    10441 ttcaacgaat caaagagtta acagaattac aaggaacaag cggttttgaa caagatgttc
    10501 gcgcttatat ggaaagtcat atggaaccgc tggtagatga gttacagtta gatggtttgg
    10561 gcggcatttt tggtctgcgc catcatgaag aagcggatgc accccgtgta atggtcgcag
    10621 cacatatgga tgaagtcgga ttcatgctga cacaaatcca agaaaacggg ctttttaaag
    10681 tcgtacctct aggcggctgg aatccttacg ttgtttctgc tcagcgtttt acacttaaaa
    10741 cctctactgg taagaattat ccatgtattt cttcttccgt tcccccacat ttattgcgag
    10801 gaacaagcgg acaaaaatca gtagaagtaa ccgatatttt gttcgatgct gggtttgaat
    10861 caagagaaga ggcaatgagt tacggtgtcc ttccaggaga cacaatcgtt ccttacgctg
    10921 aaacgatcaa aacagccaac ggaaagaata ttatcagtaa atcatgggac aaccgctatg
    10981 gctgtacaat ggtattagaa gctttagaag cgttgcaaaa tgaaacatta ggtcatacat
    11041 tgattgcagg cgcgaatgtc caggaagaag taggcttgcg tggttctaaa gcttctgtca
    11101 ataaattcaa gccagatctg ttctttgcag tagactgctc tgcagcagat gatacagtga
    11161 cgaaaaatgg cacattcgga catttaggag aaggtacatt gatgcgtatc caagatccag
    11221 gtttgatcat gcttcctcgt ttgcgcgagt acttgctgga tatcgctgaa acaaacaata
    11281 tcccttatca atactttgtt tcaaaaggcg gtacagatgc aggtgctgct catacacaaa
    11341 acgaaggaat cccaagtaca gtgattggcg tagtgggacg ttatatccat acgcatcaaa
    11401 cgatgttcag tattcgcgat tttgaagctg cacgagaaat gttgattcaa acactgaaag
    11461 gtttggataa atcaacagta aatacgatcg tatacggaaa ataatgcgat agtgaaacta
    11521 taaaatctgg caaaagattg ctttttgcca gatttttttt ataaaaagag gatcaatgtt
    11581 tgttagttcg aaagaaaggt gtataattta attgaaaaat acataaacag aaagtagagg
    11641 acaaagaatg attatcccaa atagtttaga agaattagca ggctacgtag aaactggaaa
    11701 gagtgtattt ttctttactg ccggctggtg cggagactgc cgttttatca aaccgcaaat
    11761 gcctgaaatt gaaaatgact tttctgattg gcaatttatt gaagtagacc gtgacaagta
    11821 catagatgta gccgctgaat ggaacatttt tggtatccca agttttgtcg tgatccaaga
    11881 cggaaaagaa ttaggtcgtc tagtgaataa agatcgtaaa acaaaacagg aaatcgaatc
    11941 gttcctagaa agtgtaacaa aaggctgaca tctgttagga ggggtcaaga tgaatcaaga
    12001 atataagaat attttagtag gtatcgacgg tagtgaacaa gcacttgctg ctttcaaaaa
    12061 agcggtagaa gtggctcgcc gtaataatgg gaaagtatat gtagcaaacg taatcgatca
    12121 gcaattttat aactttatgg gctatgcacc tattgatcaa aatctagtcg atcagcaaac
    12181 tgaaacagcc aaggaaatga tcgaagaatg taaagaatat gggaaatctg ttgattacac
    12241 aaatatagaa ggaattattg catatggttc tgttcgtgaa tccatggcag tcaaattgcc
    12301 aaaaaaatat gagatcgatc taatcatggt tgggcagtca ggtctaaatg cagtcgaacg
    12361 ctttatgaca ggaagtgtag ccagctacgt gatacgtcat tcaccttgtg atgtcttgat
    12421 cgtttctgac gaagaagaac agccaagtaa ttaagcaagt ggaacttgta cgaccttttt
    12481 aatcaattaa ctaaaacagt aaagggactg tgacaaaagt cttgtcacag tcccttgtta
    12541 cgaataaatg gtgttccaga agaggcttct tcggaaataa agcatgaaaa aaatttcatg
    12601 cagtgaatgc aattttttgt taagataaat ctcagcaaag gataatggag gaaagaacat
    12661 gatttttgct tataacaaag cacacgtcgg agacaccttg atggttatta tagccgacga
    12721 taaaggaaca gaaaacactg ttgaacgcaa atggaatgtt gctcggatca aagatgaaca
    12781 aggtcaagtt gttgcttgga actttttcca tatctccgat catctaacaa tcgaagggaa
    12841 cggccaagta agcttaacag atgaggaatt agcgaaattg aatcgattga tcaaagaagc
    12901 aggattcgaa gaacagcttg aagctgatca tgaaccgaag atcgttgtcg ggtacgtaaa
    12961 aacatgcgtc ccacatcctg attctgacca tttatcgatt actgagacag aagtagacaa
    13021 tggtacagta ttgcaaatcg tttgcggtgc acccaatatc gaagctggac aaaaagttgt
    13081 cgttgcaaaa ccaggagcta tgatgccaga tggaatgatg atctggccag gaactttgcg
    13141 aggggtagat agctatggaa tgatctgttc cgctcgtgaa ttgcatttgc caaatgctcc
    13201 agcaaaaaaa ggaatcttag tattgcctga ggatgccgta gtcggagaag catttccaaa
    13261 ataaagaggt tgtgacaagt ctttgtcaca acctcttttt tattaatttt cactattttg
    13321 attttgtttt aatgccgatt gatcgatcgt caattcaaca gtagttgttt tttctttcga
    13381 gccttcatag taagtgattt tgacagaatc tcccactttt ttcttataca aagcagattg
    13441 aagttctaca ccagaagaaa cttctttgtc atcgatctta gtaatgacgt cgtattgttt
    13501 caatccagct ttttcagccg gtgtagctgt ttggacattt gtcacgatga caccgttagt
    13561 tacagatgaa ggaatcttca agatttgttc ttgttgctga gtagaaacgg cagataagtc
    13621 gaccattgtg atacctagag ctggacgtgt cacttttccg tctttttcca gttgattgat
    13681 gatattcact acatcgttac ttggaatagc aaatcccatt ccttctacgc ttacattaga
    13741 agtagattca gaagtacttg cgatcttact tgagttgata ccgataactt gtccttcgat
    13801 attaactaaa ggaccaccag aatttccagg gttgattgct gcatctgttt ggatcgcatt
    13861 gatattgact gtttcgttcg attcattcgt acttgtcact tgtcggttca aggaagaaat
    13921 gattcctgaa gtgacagaat ttgcatattc agatcctagt ggggaaccaa tcgcaatcgc
    13981 aggctcacca accttcagag cacttgagtc accaaaagag gcaactgttt cgactttgtc
    14041 tgaattgatc ttcaacaccg ctaaatctga ataagcatct gttccaacta attcagcttt
    14101 taccttcgta ccgtctttca tcaagacttc aagaccttgc tggccatcta ctacgtgatt
    14161 attcgtgact acataagcag tattcccgtc ttttttgtag atcacgccgc ttccttcact
    14221 ataagcttcc aaattactat catctgagct gctactttct tcttgctgcc cgaatagtcc
    14281 gccaaaaccg cttgattggt tttgactttg taagttgata acagaaacga cagcgtcttg
    14341 aactttatct accgctttgg tgatatcaga atctacattg actttgacat tttccacaac
    14401 tgtttcaccg gcagaattct gattgccgct tgtagcagta gaactattgt tgccagtgcc
    14461 catcgctgca taaaaaatgc cagctgtcag tagtcctcca acgatccctc caacaagccc
    14521 cagacctaac ttacgccaga ggctattatt tttacttttt ttcatctttg gtgtcacatt
    14581 ttttctatcc atgtgttcct cctccaatta cattcattta tctactgtta gtatagtctt
    14641 atttgttgac tttagtctac tttgagactg tgaacttttt atgaaaaact ttaggaaaaa
    14701 tcattgtttt cagatgaaaa tatttgattt tctttttcca cagatactgt gtaaaactac
    14761 gcggatagga ttggaaaaat aagggataaa acatatccac agcctgtgta taaagtggat
    14821 aaattcttat aaaacgtatg tttgcctaat gaatgtgatg aaataaggta aaataacact
    14881 gttttctgtg gataaacctg tggatgttgt gcataactgt tggggaaatc tttttaacaa
    14941 aaatagccaa aataaacata tagacgaaaa aattgattat aataaacaaa gtgagcatga
    15001 agaccaaagg agagaaaaca tgaatatcaa aattatctca gtaggaaaat taaaagaaaa
    15061 atacctgatc caaggtatca atgagtatgt caaacggctg aatgcctatg caaaaatcga
    15121 attgatcgaa gttcctgacg aaaaagcccc ggagaattta agcgaagcgc aaatgagaca
    15181 agtaaaagaa aaagaagggg aacggatctt agcgaaaatc aaagaacaag aatacgtcta
    15241 tgccttagca attgaaggga aaaatcctac aagtgaatct tttgctaaac aaatcgacca
    15301 attaggtatc caaggaaaaa gccaccttgt ttttgttatc ggcggctctt taggactcag
    15361 cgaagccgtc atgaaaagaa gcaatgctca gatctctttt ggaaaaatga cgtatccaca
    15421 tcaattgatg cgtctgatcc ttgtcgagca aatctaccgt gccttccgga tcaatgcggg
    15481 ggctccatac cacaaataat gatatttttt aagagaaaaa attatcccta ttttaaataa
    15541 tagcaatagg tagtggtagg gagaaattta taaataactt ttttatttgc tttggtagcc
    15601 aagtaagttg caaaagaaaa atagatactg ttaaaaagat aatgtagaca tagaaacaaa
    15661 tacgtaaaat tttagttaga ataatggcaa ggagaaaaaa catgatagat gaaaaaaagt
    15721 gtttaaaaaa tctattagag gatatggatg atattgaaga aaactatcta aatggactgg
    15781 gaaaacaaat atatttcaga tacttaagct atctaaaaat gaaataagac atagtaattt
    15841 tttagctttt ttgtttgctc caaatgaaac ccacaattta tctgatgagt tttttaaaat
    15901 gtttttgaaa aggtatatag attctaatga tgataccaaa gctgctatca attattttga
    15961 tgctttactt aatagctatg aagatttaat tgtttacaga gaaaataaga atattgatat
    16021 tttattggta tcggaaaaaa ataaaatagt ggtgtgcatc gaaaataaaa ttctgtcttc
    16081 ggagtctgga gggcaactta ataaatacca aatatatata gaacgcaatt attctaacta
    16141 taaaaaaatt tttgtttttt taactccaga tggaaatgaa cccacaaatc ctagttgggg
    16201 cataattact tacaagaata tcgttgaaga gagtgtaaaa tattttgtgt aaatagaaaa
    16261 aaggaagtcc cttctgtaga atagagttac cacaacacat tcacagaaaa gaggacttcc
    16321 atatgaacga ttttactaca gaaattatgg aaatactaat caataaaggt gatttggatg
    16381 atttgtttcg tcgtcaccta gaattggcta ttaacgcttt attacaagct gaattaacgg
    16441 tatttcttga ctatgaaaag tatgatagag ctggatttaa ttcaggtaat tcccgtaatg
    16501 ggaattattc gcgctctttt aaaacagagt acggtgaatt gaatttagta atacctagag
    16561 ataggaatgg agacttttct caacaaactt tacctgctta taaaagaacc aatgactctt
    16621 tagaaaccac tattattcag ctagttcaaa aaggaattac tatgtccgaa atctctgatc
    16681 taatcgaaaa aatgtatggt cattattaca aaccacaaac tatttcaaat atgagtaaaa
    16741 ttgtgtctga agatgtttcg gcatttaaag aaagaacttt agaagctaaa tactcagtca
    16801 tttttatgga tgctactcat attcccttaa aaagacaaac cgtatcaaaa gaagctgtct
    16861 atatagtaat tggtatcagg ttggatggaa ctaaagaggt tctaggattt actatagctc
    16921 caactgaatc tgcttatgtt tggaaagaga ttcttcaaga tctaaaagac cgtggtttag
    16981 aagaggtttt gttagtcgta acagatggtt taagtggtat tgacgatagt attcatagtg
    17041 tttatccaaa tgctcaattt caacaatgtt gtgtccatat atctagaaat atcgctcata
    17101 aggttcgtgt tagtgatcga caagaaatct gtagcgattt caaattagtt ta
//
LOCUS       DAJSBL010000057        17121 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.059, whole genome shotgun sequence.
ACCESSION   DAJSBL010000057 DAJSBL010000000
VERSION     DAJSBL010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17121
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.059"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            34..240
                     /locus_tag="ONT85_002226"
     CDS             34..240
                     /locus_tag="ONT85_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2379180.1"
                     /translation="MKQKVVGEVISKLENCVVICVDEYHDVDRNEILEKCGKVVAKYT
                     HVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(602..841)
                     /locus_tag="ONT85_002227"
     CDS             complement(602..841)
                     /locus_tag="ONT85_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2379181.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
     gene            1127..1441
                     /locus_tag="ONT85_002228"
     CDS             1127..1441
                     /locus_tag="ONT85_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2379182.1"
                     /translation="MDFYSNFILIIAILLLLNIWFFDKSRNAGIGFRTKRSTSSEKKW
                     VYSQTIFYGGVISISLLSSTLYSFNVIDVSMSNFISIIGILISAIITQLLLVFEEKSK
                     NN"
     gene            complement(1875..2241)
                     /gene="ssrA"
                     /locus_tag="ONT85_002229"
     tmRNA           complement(1875..2241)
                     /gene="ssrA"
                     /locus_tag="ONT85_002229"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(2412..3185)
                     /locus_tag="ONT85_002230"
     CDS             complement(2412..3185)
                     /locus_tag="ONT85_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCR2379183.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMGNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            3329..3679
                     /locus_tag="ONT85_002231"
     CDS             3329..3679
                     /locus_tag="ONT85_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HCR2379184.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            3660..4376
                     /locus_tag="ONT85_002232"
     CDS             3660..4376
                     /locus_tag="ONT85_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR2379185.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAAVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            4376..5824
                     /locus_tag="ONT85_002233"
     CDS             4376..5824
                     /locus_tag="ONT85_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HCR2379186.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(5894..6403)
                     /locus_tag="ONT85_002234"
     CDS             complement(5894..6403)
                     /locus_tag="ONT85_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2379187.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(6393..7118)
                     /locus_tag="ONT85_002235"
     CDS             complement(6393..7118)
                     /locus_tag="ONT85_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2379188.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHLQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWRLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
     gene            complement(7341..9461)
                     /locus_tag="ONT85_002236"
     CDS             complement(7341..9461)
                     /locus_tag="ONT85_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HCR2379189.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLAKGKNVIYIHLESFQQFLIDYKLTDENG
                     LQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYTSAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            9691..10869
                     /locus_tag="ONT85_002237"
     CDS             9691..10869
                     /locus_tag="ONT85_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HCR2379190.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIVGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            10885..11643
                     /gene="aroD"
                     /locus_tag="ONT85_002238"
     CDS             10885..11643
                     /gene="aroD"
                     /locus_tag="ONT85_002238"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HCR2379191.1"
                     /translation="MVWVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLAGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            11666..12151
                     /gene="ybaK"
                     /locus_tag="ONT85_002239"
     CDS             11666..12151
                     /gene="ybaK"
                     /locus_tag="ONT85_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HCR2379192.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(12222..13475)
                     /locus_tag="ONT85_002240"
     CDS             complement(12222..13475)
                     /locus_tag="ONT85_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCR2379193.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            13593..14942
                     /gene="gdhA"
                     /locus_tag="ONT85_002241"
     CDS             13593..14942
                     /gene="gdhA"
                     /locus_tag="ONT85_002241"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HCR2379194.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNVQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            15054..16400
                     /locus_tag="ONT85_002242"
     CDS             15054..16400
                     /locus_tag="ONT85_002242"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HCR2379195.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(16708..17094)
                     /locus_tag="ONT85_002243"
     CDS             complement(16708..17094)
                     /locus_tag="ONT85_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HCR2379196.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYPEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
BASE COUNT         5615 a         2991 c         3414 g         5101 t
ORIGIN      
        1 ttaggaaaaa aatatatttg ccaacctatt ggcttgaaac aaaaagtggt tggagaagta
       61 atcagtaaat tagaaaattg tgttgtgatc tgcgtagacg aatatcatga cgtcgatcgc
      121 aatgaaatat tagaaaaatg cggaaaagta gtggcaaaat atactcacgt ttatgagttg
      181 gaaacaacag aatatctata taactatagc aacaaatatg aacatacttt agtttcataa
      241 agatttaaat aaagtgcctt caaaagcgag aagaccaagc ttttgaaggc actttattta
      301 gttggcagtg attgttcatg atttttgatt tattcaaaag gttcgcctag agaatctgtc
      361 tctgtaacga ggcctggtgg agaaactcga taaaaaccta tactgccaga actgtcctat
      421 tttcggatag acaaactagt agttttaaaa agatcatccg atccaatcat ttcaaaaaat
      481 gcaaaactga tatcaggatc atttagctca ggcttgttat cgtaaagcat ttgtaaaggt
      541 tgcatttagt taggagtaaa cgcgaaacta tcatcattat ttgctgaaga agatgaagaa
      601 gctaattcag agtttgtttg aaataaagga gacgtgctga cagtttcttt accctgtttt
      661 ttgcagaggt agaaactttt atatcggaag tttctaccgg agaatttttc tcatttgtag
      721 aaattgttga atcgatacta ttcgaaattt gcgtatctga aggtatgttc tttgatgcag
      781 aagtcccaca agcagtaagt aacaataaat ttgataaact gataaacaga attcttttca
      841 ttatgtacac actccttata gtatcgttta gcatacacct atgctcagta ttaggcaaaa
      901 tcaatgcaaa actatcaagt cttttatagt atctattagt cttagtaata catatatgga
      961 gagggtgata tcccagttca aaccagatac attggttacc atctgtatta acgattatgc
     1021 ctctaaacgc tttttttatg aaaaaattta taagtgaaaa cgtaggtatg atattatcta
     1081 gaggtaattt ttttgggtat ttaaatagtt gctaggagga tagcttttgg atttttacag
     1141 taattttatc ttaataatag caatattatt acttctaaat atttggtttt ttgataagag
     1201 tagaaatgca gggataggtt ttagaaccaa acgaagtacg tcttctgaaa aaaagtgggt
     1261 atattcacag actatttttt acggaggggt tatttcaatc agtttacttt cttcaacact
     1321 ttattctttt aatgttatag acgtttccat gtcaaatttt atatctatta ttggaatcct
     1381 tatttctgca attattacac aactacttct tgtgtttgaa gagaagtcaa aaaataatta
     1441 aagcaattat gccttttagt acaggaatta ttacttctta caaatgtata gcgaatgtga
     1501 agcctgctgt tttaattgtt ctagaaaaac ttttgagtga acaattattt tatgttagga
     1561 tgaattaatt tctctagagt ttataattaa aaaacttatt tgaaggaaca tgcttaaacc
     1621 ataattgtgt ttttccttcc atcagcataa aagttcacct caaaaagagt gtacgaacaa
     1681 aagttctttt tgtaaacctt aaaatagatt ttcctgaata cgtattgggg atttctaaat
     1741 gtttgaatgt ttttttgaat gcaaaatcaa tgcaaaaaat tttgtatttc tctacttttc
     1801 tctccttgac caaaataaaa aaacgttgat ttaacaacgt ttttcataca tgtttatctg
     1861 tctcaaccat acatatatgg agacggcggg agtcgaaccc gcgtccaaac acatcgccac
     1921 ttaaaaatct acgttcatag tcaactcatt taaggattca ctttacagct tgccgagtga
     1981 caggcattct gtatcgctag tctgatgttc tctttttcaa cttacagacg gaaagttgaa
     2041 acgtatccca ctaaagttag gacccttact cgagcacatg ggcgatgccg agaggatcta
     2101 cgctaactgt ttttaggcag ctaaagcgta agagttgttt tcgtttttag cagttatatt
     2161 taactgtaac gtttttacgt agacgtaacc tacgaaacgc aattcaagct cgactgtgcc
     2221 tgtcgaatcc gtaacgtccc cgttagctaa caagggttac gtgtattttt catacaaaat
     2281 tagtatagca tatattggga taaattcaat aaaaaagcga atcatatccc attatttata
     2341 taataactgc ccctttctta agaaaaaata agcgattttc ttaagaaaag aggtagcttc
     2401 aaaaaaagat cttaaatcag atcaaaatac ttcaatgcgt ggacaattcc gccttctgta
     2461 tttttctttg tgataaaggt agaaagttct tttaatccgt cataggcgtt gcccatagcg
     2521 attttgttgt cgcaagcttc caacaaggca aaatcattcg gaccatcgcc aaatccaaaa
     2581 gtaggaactt tttccagctg aagttctttt tgcagacgtt tgacacctgc cccttttgag
     2641 attcctttct tcactgtatc gatcgagtaa ggaccattcc gataaaaagt tagctcagga
     2701 aaatgttcgt gatagtattc gtcacctgat tgagataaga tcagcaacat atttactgtg
     2761 ttttctttaa agcctaaagg atcaatttcc ggaacatctg aatgaatgaa gtcgtaagca
     2821 ttgatcattg tttggctatg accggtacac cagattttct gatcgttata gtaagaaagt
     2881 tcatgaccgt tcaactgtac atgatcatac attttttgac attcttctga agaaaatgtt
     2941 tgagaataga tttcttcgcc gtctactcga atgaaagaac cattcatggt gaccgcagaa
     3001 gtaatgcccg catcttccat gatagagtgg atttctgctt ctgtacgtcc tgtagcgatg
     3061 acggggagaa cattgttttc ttttaatgcg ttcatagctt ttgccacttc gggtgtgatt
     3121 tttgatttcg catctagtaa tgttccatca agatcaaaga aagtgattgc acgataattt
     3181 gacacaaata aaactccttt tttattggaa taagtttatc ttaacaaaga atgaacaaaa
     3241 gacaaacctt tcgtgaagaa ttcctgaaca tattggttca gtcaacaatt tagagtatac
     3301 tataagaaga gaatttagga gggaaagaat gatttttttc agtattggta tcttattctt
     3361 aataattggc agtctttttc ggattctgcc ctcaaaagga aatctgcctt tttatgggta
     3421 tcattcgcct cttgccgcaa aaacagacgc tcattggcgg ctggctcaaa agacaagcgg
     3481 aaactggttt tttcttatgg gcttactgat ggcattgatc ggctattatt taaagacatc
     3541 tgggcatacg aattattttc tgattgaaat gcttctgcta gtatttccaa ttatgcctat
     3601 ttttattatg acagaaaaga aattacagaa atatgacctg gaaactggag ggaacgataa
     3661 tgaatatttt aatgattgaa gataatcaat ctgtttctga aatgatgcag atgtttttct
     3721 taaatgaagg ttgggaagca actttcaagt atgatggaaa agaagggctc gatgcctttt
     3781 tagaaaaccc acaacattgg gatatgatta cactagacct gaatttgccc acactggacg
     3841 gtatgctagt aggaagagag atccgcaaag tttcaagtac tgtgccgatt atcatgctga
     3901 cagctagaga ttctgaaagc gatcaggtga tcggcttaga gatgggggca gatgattatg
     3961 tcacaaaacc tttcagtcct ctgacgctta ttgctcgaat gaaagctttg catcgccgta
     4021 gtgagttggc agaagcaaga gaagaagcag cagtttcaga tgatcatttt gatatcgaga
     4081 ctagtcattt caagatgaat acgaaaacac gagaagcgta tctggacaac cggccaattg
     4141 aaggactgac gcctaaagag ttcgatctat tgtttacttt agcaaaaaaa ccacgtcaag
     4201 tattctctcg ggagcagttg cttgaattag tctgggatta tcagtacttt ggtgatgaac
     4261 ggacagtgga cgcccatatc aagaaattac gacaaaaaat cgaaaaggtc ggaccacaag
     4321 tcatccaaac tgtttgggga gtcggctaca aattcgatga ttctggtgtt gcctaatgcg
     4381 ttatttgtat cagcaactct tagctttttg cggaatggtc gcgttgatct tattgatcgt
     4441 tggagtttct tttacacaac tgacaaaaca aacgattgaa gaaaacaatt accagcaact
     4501 gtttggatat gctgaatcag ttgaaaagac aacgcaaacc tatgccgata cgctcccgca
     4561 gtttagtcaa gatcaggcgt ttcaaaatgc attgctgctg acagagcaag tactgacaga
     4621 acaaaatgtg aactttatat ttatcgataa aaacgaacgt gtcatttatc cgactaatac
     4681 agctttactg aattttacct taacagctga tcaatggaaa aatctgagga atggacgtca
     4741 agaaaaattc acatcgaata aaaatatttt aggacaagca caggcgacct cttacgcact
     4801 tgtgccattt aatttgaatc atgaattcta tggagcactt gtagttagtc aacctgcgcg
     4861 aaatatcgat aacagcgttc gtccaattac actgaacctg ttcaaaggat ttattttttc
     4921 cagtatcgtt gcagttattg ccagctatgc ttttgcagca ttccaagtta agcgtatcaa
     4981 tcggttaagg aatgcgacga aagaagtgac gaatggtaat tttgatgtac agctccccgt
     5041 acatgacaaa gatgaatttg atgagttagc agatgatttc aataaaatga cgaattcatt
     5101 gaaagaatct caagcagaaa ttgaagaaca agaaaatcgt cgaagacaat ttatggcaga
     5161 tgcttctcac gagatgagaa cacctcttac gacaatcaac ggacttctgg aagggttaga
     5221 atacaatgca atccctgaaa atcagcgaga aaacgcgatt aaattgatga aaaatgaaac
     5281 agagcgtctg atccgtttgg tgaatgaaaa cttggattat gaaaaaatcc gtacgaatca
     5341 aatcagtatg gtcattaaaa aattcaatgg aacagaaaca ttgaaaaata ttgttgcaca
     5401 gttagaagct aaagcagaag cagcaggaga tacattgaca ttgaaagccg ttgatgatat
     5461 tgatgtttac gctgattatg accgatttgt ccaaatcatg gtcaatatta ttcaaaacgc
     5521 gatacagttt actgaaaacg gccaaatcac gattacttta gaaaaaggct atcttgaaac
     5581 gatcatcacg attgaagata cgggaattgg aatgtctgaa caacaaatga aaagtatctg
     5641 ggatcgttat tataaagtag atccttcaag aaaaaataca aaatatggtg aatcaggatt
     5701 aggtttacca atcgtgcagc aacttgtacg cttgcataaa ggaaaattag aagtagaaag
     5761 cgaactagga aaaggaacaa aattcgctgt tcgtttgcct gacgtagaaa tcaaaaaaga
     5821 gtaaaaaaca agcggtataa aagatcatga atcgatcttc tataccgctt gtttttacta
     5881 taaaatgatt gatttagccc gcgggctcta aacgatcaat ataaggactt tgagcaatag
     5941 tagcatcatg agtaaagagt aagatacctt gattttcttc atgagctaaa taagaaagca
     6001 agtggagtag ctcttggatc tccaatacac taagttcatc aaatgtatct tcaataacaa
     6061 tattgtttcg cttggaaagc aaggcatgaa gtaattgcag ctttactttt tcaaaagacg
     6121 tcaactcagc tgccgcacaa ttaagcaagg aaggagagat atcaagtttt tcttgatagg
     6181 ccaaacgatc ttgtttgatt tctttttccg gtacatccaa caggacatgg tctttaagat
     6241 tcagataagg aaacatcggc caattttttt ggatgatcgc aaaattatcc agttttgcaa
     6301 caagaacctc ttttttcaag tgctccaata ttttatgctg tttttcgaga ggacacaaaa
     6361 taacggtcaa gtgttcagca tgaatcaaat attcaggttt cattacgtcc aggcctccta
     6421 ctttgcaaat gatggataaa aaaagtgaaa gggaatagac taagaatgag acagcatcca
     6481 ctgagcccaa taagagtttg ggcaaatccc ttaagtatat ctgtgaaaaa aagatcattt
     6541 tggaaatcga tggtaaaaaa agtctgttga tccatcggaa ttgtaagtcc gcttaccgaa
     6601 gtcaagatat cacggatatc tggcagttca gtagtcttta agacaagaaa attgatcctt
     6661 tgaagaagcg cttcataaac tgactggaag cttacgatcc acaataaaac caagatacaa
     6721 caaagaaaga gaggaaagaa cagctgccag aaaataaaaa ggaataattt ggttttagaa
     6781 agaccagtca ggcgccaatt gatcaattcc tgcttgtttt gcctgactga atagtaaaag
     6841 aaaaagataa aaaggaaggc aaatagaaaa aataaactga atatgccttt tatataaaat
     6901 cggctagcta attctaaagc cggaagaagc tctgtagatg cagaagcgcc aataaatgaa
     6961 agccgatctt ggacttgatt ggctaaagcc gattgcaaat ggtaaagagt caggcatgct
     7021 atcaataaaa aagcagaaaa gcttgaaaat aaacaataga gtattgtctg ctttttgtaa
     7081 taaattaatc gagcagtagc atagcggatt attttcatac ttttcacctc tataaaaagt
     7141 atagaaaatg aatatgaata aagtatgaaa ttcctttagt gaaatcacga attaaaacca
     7201 gaaaatattt tgtaaaaagc aagaggttgt gaagaagctg ccctattcta ttaccgtaga
     7261 tgcaagcttt tgaacactgt ttattcgaaa agaggagcag cgacaataat tttgtcgctg
     7321 ctccttttaa atttattttg tcattcggaa gaacttgtgg tcgttccgtg aagttcttgg
     7381 tacgtttgcg ttttataaag atcaacagtt gattgtccgt tgtgttcact aaagacacta
     7441 gttgacttgt caccaagaga tttttcagct tcttttaatt tctctaagcc attcttgtag
     7501 tcataatcac tggtatcaat tgggtctaat ccactattcg tatagaaccg aagaagatca
     7561 ccattattga tttggtcaga aacagctaac tgtttatcaa ctttttcttt cagtgaatca
     7621 atttcattct gtacttcctc agtaggttca gtaatagctt caccagtttc gttactatag
     7681 atcgtaccac cgtagtaagt atattttggt gtaacaaaat caccatcacg gaaagaaaca
     7741 agctgttcat gatctttcga gaatagatct tgtcctaact gaatataatt ttttgtatct
     7801 acacctagta agtgaagtaa tgtaggcaag gcatcaactt gtccgccgta agtgtgattg
     7861 attccgccct gtgtttgtcc agggatatgg atcatataag ggacacgctg catttgtgca
     7921 ttgtcgtagt ttgtccaagt agaacttgtt tttccaacta attcagctag atttttattt
     7981 cttgaattcg agacaccgta atggtcaccg taaaggacga tgactgaatt atcatacaag
     8041 ccgctttctt tcaaataatt gaagaattct tgtacggcgg tatctaaata attggctgtc
     8101 gcaaagtaac cgttgatcgt ttcatcattt gtattggcta acgggaatcc tgcttcgtca
     8161 ttggtgaatt ttgaataagg ataatggtta gatacagcaa tgaattttga ataaaatggt
     8221 tgttgcagac gttccaagta ttgtacggat tgttggaaaa atggtttatc atgcaacccg
     8281 tattggaacg aattgtcatc attaacatca taataagaag aatcaaagaa ataatcatat
     8341 cctaaattct tgtatgtctc gtttcgattc cagaatgtac cagcgttccc atggaatacc
     8401 gcactagtgt aaccttgtgt ttgacttaaa atatcaggtg cagcttggaa agtatttttt
     8461 ccgcctaatt gtgtgaacaa ggagccttga ttcaagccaa acaatgaatt ttccatcagc
     8521 gtttccgcat cgcttgtttt tcctgctttt acttgatgga aaaagttgtc aaaactgaac
     8581 gtgctgttgc tgtggtaaag actattgatg aaaggtgtaa cttcatgctg gagaccattc
     8641 tcatctgtca atttataatc gatcaggaat tgctggaagc tttctaaatg gatataaatc
     8701 acattttttc ctttagcgag cccgaacaaa tcgtcatttg gtgctgcata atgttctttt
     8761 acataatttt ctacttcttt catatcattg ggacttgctt ctgcccgcac ttggtttgtc
     8821 tggtaagttt gaacaccgtc ataaaccatg aaagcattca agccaagata tttgaccaaa
     8881 taatcacgag agaatgttcg agtcagcaat tctggacgat cagcttccgc cataaaaaga
     8941 tttcctgaga aaatcataac tgctaatgta gagatcgcaa aagccattct tgctcggatc
     9001 gggcgagggt ccaacttgat tttttttgca atcagcagaa cagtcaagat aataaaatcg
     9061 atccaataga tcacatcata tgggcgaaac agtcgaatgg cactttctcc cagaccactc
     9121 gctactttcc cagcacctaa aatcgtgttg atcgtaatga agtccgtgaa ttctcgataa
     9181 taagtgacat tcgaaaaaac aagcaatgtc aaaagaaaat aaatgaccat catcgtgata
     9241 taagcaactc tagggcgtct gatataaaga gcaattccaa gtaataacaa tgtagttgca
     9301 atagggttga taaacaagat aaagtattga atgccacttt cgattccgag atggaaatca
     9361 actaaatagg caaaaatcgt tttcaaccaa agtaaaacga ctagtaaaga aaaaaatccg
     9421 agcctcttat tcaaaaaggc aggtgttttt atacatttca agataatctt cctttctgca
     9481 cttcttaatc acagaagtgt catgaaacca tgttacacag ctcacttatt ctcatttatt
     9541 ttactagtat gacaatatag tgtcaattga aaccagaagt ttatgctgtt tcattaaggt
     9601 tttctttaag cttcatgaaa aaatgagcag taagacaagt cctatgtttt ttgttatact
     9661 tgggaagaga aagctaaagc aggtgaagga atgaaagtaa aagtaaaaaa aagagcaagc
     9721 aatcgattga aaaaaggata tccactgatc caaaaagaag accttgctca tccccaaaaa
     9781 gaaaccacgc aatgggtgga attagttgat tcgcaaaacc aatttatcgc aaaaggatat
     9841 ctaggaaagc agaataaggg aatcggttgg gtattgagcc agcagaacat cccatttgat
     9901 caatcttttt ttgaagaatt ttttgtgaag tcaaaagaaa aaagaacttt ctttttttca
     9961 gatgattcta ctaccgcttt tcgtatattc aatggagaag gagacggaat cggcggtctg
    10021 attgttgatt attatgcagg ttatgctgtt ttttcttggt ataacgaaac gttatatgca
    10081 catcgtgaat tattgattca agcttttcgt gaaatctacc cggaaatcgt aggaggctat
    10141 gagaaaattc gatttgaatc agtaaatctg cctgagagcc agttcattta cggaaaagag
    10201 gcaccggaac ccttgatcgt aaaagaaaac ggagtaaaat ttgccgttta tttaaatgaa
    10261 ggattaatga ctgggatctt tttagaccaa aaagaagtac ggggatcatt agcagaaggt
    10321 ttcgcttctg ggaaaacagt tttgaacatg tttagttata ccggcgcctt ttctgttgct
    10381 gccgcaatgg gaggggcaat cgaaacaaca agtgtcgatc tagcaaaacg cagtttaaaa
    10441 aaaacacagg aacagtttga agtgaatggt ttggatgtcg atcagcaaaa aatcattgtt
    10501 atggacgtat tcgagtattt caaatacgcc aaaagaaaac aatttttata tgacatgatt
    10561 gtattggatc caccaagttt tgccagaaat aagaaaaaag tctttcgcgt agcaaaagat
    10621 tatggggaac tggtcaagga tagtctggat attttagcag aagacgggat acttattgcg
    10681 tctacaaatg ctgcgaatgt ctcgatagaa caattccaaa caatgattga agaggaattc
    10741 catgccaaaa atgtttcttt tgaacaactt gctttatatc gtctgccaaa agatttccag
    10801 acgacggata cctttttaga aggcaattat ctgaaagtgt ttgtttatca aattaaaaat
    10861 aaccattaga aggagcgatg cagaatggtt tgggtaaagg gattgaatat aggaagcggt
    10921 agaccaaaga tttgtgcgcc tcttatcggg gccgtccgag aggaagtatt acgtgaagca
    10981 gttcttgcac gtgaagcagg ggccgattta gtcgaatggc gagtcgatca ttatcgtgaa
    11041 gttttttctt ttgatgaagt agcagaaaca ttggttctta tccatgagat gcttgatgga
    11101 ctgccgttat tatttacgtt tcgtacctta gctgaaggcg gagaacaaga catatcgatc
    11161 cgtcagtatc gcgaccttta tgaacatgtg atacacacag gattagtaga tatggtcgat
    11221 atcgagttgt tcaaagtaga aagtctagga aaaagactga tagaggaaat caagtcttta
    11281 aacattcctt taattatcag tagtcatgat tttaaagaaa caccagcaga ccctgtcttg
    11341 ctttaccgat tgaatgttat ggaacatttt ggagccgata tcggaaaact ggcagtaact
    11401 cccaataatg aacgagatgt tttgcggttg atggagttga ctagacgggc aaatgctttt
    11461 gtctccatgc ctattattac gatgtccatg ggaaatcttg gtaaaatttc tcgtctggct
    11521 ggcggaacga cgggttccgt tatgacattt ggttccttaa gcagcgattc tacttctgct
    11581 ccaggacaat taccggtcaa agcattgaag caaattattc aattattaaa tgaaccgttg
    11641 tagaacaagt ttgaggtgat attttttggc aaagaaaaaa atagtaaaaa cgaatgcaat
    11701 ccgtatggtc gaacaaaaaa agattcccta tacagagcac gaatatgagt gggacgaaag
    11761 ccacctaagt gcgtcatcgg tcgctgaaca gcttccagaa agccaatctc gtatatttaa
    11821 aacactggtt gctgtgggca atgtgacggg gcctcttgtt gctgtgatac cgggagaagc
    11881 cgaattgaat ctaaaaaaat tagcaaaagt ttcagggaat aaaaaagtag aaatgctcca
    11941 tttaaaagat ttggaagcaa cgaccggata catacgtggt ggctgctcgc ctataggtat
    12001 gaaaaaacta tttcctactt atttagatca aattgcagaa agctatgaac aaatcattgt
    12061 ctcggctggc agaagagggc tacagatgga gttagcacct caagatatct gtgcattaac
    12121 gagcggacaa tttgcagata taaaacaata aatcatacgt ataaaagaaa gaaggaggcc
    12181 gggatgttgt ttgtccagtc tccttctttc ttttttatac attatgctct ttgcccggca
    12241 ttcattcctg ctacatgccc tgtagcgaaa gccgctgtga tattaaagcc gccagtataa
    12301 ccattgacat ccagtaattc tcccgcaaaa tacagaccat caatgatttt actctccaat
    12361 gttttaggat aaatttcctt tagagaaacc cctccgccag ttacaaatga tttctcaata
    12421 ggaaaagttt tgttgattga tagcttaaac tctttgcata gttgaacaaa atcttgaatt
    12481 tgtttttgag aggcttgttg tcctgtcaga tgatccattt caagccgctc aagaaagaaa
    12541 actaacaatc gctcaggcaa tataccatgc caagcattga ccaagttctt tttggttgct
    12601 ttactttttt ctgtcaatat atgaataagc tcttgtttgg tttgtgtagg gaaacaatcc
    12661 agagaaagtg tcactggttc tcccgttttc tctaattctt tattgacaaa acttgaacaa
    12721 cgcagtgcag ccggaccaga aataccgaaa tgggtgaaaa gtaaatccat cgtatgttca
    12781 gtcaatacac gacctttttt atttaaaacg cgtaaagtga tgtcttgtaa agaaagccct
    12841 tgaagtgttt tctcctgaat atacggttca tccgatatca agggggactc tgtcgcataa
    12901 agaggcgtca ccgtgtgacc cactcgcttc acgatcttgt acccatctcc agtagaacca
    12961 gtagatggat aagtccgtcc accagttgtt aagataatgg caggagcatg gaactcttcg
    13021 aattccgtgc gtataccgta aattttatgt tctttgtgga tcaatttctc tactcgagta
    13081 gagaatagaa gcgtcacatc tagttctttc agacgattga gcaatgcttc gataatcgtt
    13141 tttgatttat ttgttacagg aaacatccgt ccatgatcct cttcttttaa atgtacgcct
    13201 tgtgattcaa agaagttcat gatatcgaaa ttattccatt gcgcaaacgt actatataaa
    13261 aactttccat ttccaggaat atgtgcaatc aaatcatcaa caggacgatt gttcgtaaca
    13321 ttgcaccgac caccacctgt catcagtagt ttttttcctg cttttttgtt tttttcgatc
    13381 aagagtacgc gtgcgccttg ttcagctgca ctgatcgctg ccatcatccc gcttgttcca
    13441 gcgccgacta cgatcacatc atacagtttt tccattccct cacctcaagg aaagtgtagc
    13501 atatctgaag aaagaaaaca gaaaaaaaat gttttgagcc aaaagagaaa atgagctaaa
    13561 atgagggaag ataagcaaag gagcgtggaa cgatgacaaa cgcaacagaa tatgttcaag
    13621 caattcaaga aaagctacat caaaaggatc aagggcaaga agaattttta caagcaatcg
    13681 atgaattcat gcccacggtt atgtcttttt tggacactca tcccgaatat atcgaaaaga
    13741 atatcttaga acttttaacg gaaccagaaa gagttattca atttcgagtg ccttggcaag
    13801 atgatgaagg aaactggcga gtaaatagag ggtaccgtat ccaatacaac tcagctatcg
    13861 gcccttataa aggtggattg cgcttccatc ctagtgtcaa tctaagtatc ttgaaatttt
    13921 tggcttttga acagattttc aagaatagtt taaccggtct gccaatcggt ggaggaaaag
    13981 gcggaagtga cttcgatcct aaaggaaaat ctgataatga gatcatgcgt ttttgtcaaa
    14041 gtttcatgct ggaattgtca aaacatatcg gcccatcgat tgatgttcct gcgggagata
    14101 tcggtgtagg agctagagaa atcggctatt tgttcggcga atataaacga ttgaaaaaat
    14161 atgatactgg tgtgttgaca ggtaaacctt tggatttttg gggaagtaag atacgtacgg
    14221 aagctactgg ttatggatta gtgtattatg tcaaacatct attgaacgaa gaaaaagatt
    14281 catttaatca aaaaacagtt tttgtttccg gaagcgggaa tgtagccatt tatgcaatcg
    14341 aaaaagtgca agagctaggc gggaaagttg ttacatgttc agattctagc gggtacatct
    14401 atgatccaga aggaatagat gtgaaactat tgaaagaagt caaagaaatc aatagacaac
    14461 gtttgacgga atacgcaaac caacgtcctt ctgctattta tcatgctgga gaatctgttt
    14521 ggacattgaa agaaaaagcg gatatcgctt tgccatgtgc tactcaaaat gaaatagacg
    14581 aaagcttggc cgaaatctta gtagaaaatg ggatcaaaat cgtagcagaa ggcgctaata
    14641 tgccatgtac agtaaaagct gtttctgttc ttcatgaagc aggcgtgtgg tattgtccag
    14701 gaaaagctgc taatgctgga ggagtagcag tatctgcttt agaaatgagt cagaacgtcc
    14761 agcgattgac ttgggaaaga gagcaagtgg atgagcagct ggatcaaatc atggaaacga
    14821 tttaccatac gtgtcgccaa acagcagccg agtacggcga cgaaaaaaat ctgcttcttg
    14881 gagcaaatgt tgctggattt gaaaaagttg ccaaagcaat ggcagcagaa ggtttagttt
    14941 gaaaaatgaa actttcttgg aaatattgtc agcattttgt ttatttgctt gaaaaataat
    15001 aaaaaaaaac gtaaagtatt acctgtagag taaacctata aggaggagaa caaatggcac
    15061 acattcattt tgactattca aaagtagcac cgtttgtcaa cgaacaagag ttagggtaca
    15121 tgcaaagcca agtaacagca gcccacaacg aattacggga aggtactggc gcaggaagcg
    15181 acttccgcgg ctggatcgat ttaccaacga actatgacaa agaagaattt gcacgtatca
    15241 aagaagcagc taaaaaaatc caatctgatt ctgaagtatt agttgttatc ggtatcggtg
    15301 gttcttattt aggcgctcgt gcagcgatcg acttcttgaa tcacacattc ttcaatttat
    15361 tagatagcaa cacaagaaaa gcaccacaaa tcttctttgc cggaaattca atcagctcaa
    15421 catatattgc tgacttgatc gaagtaatcg gtgatcgcga cttttcagta aacgttattt
    15481 caaaatctgg aacaactact gaaccagcga ttgcgttccg tgtattcaaa gagcttcttg
    15541 ttaagaaata tggacaagaa gaagcaaaca aacgtatcta cgctacaaca gataaagcaa
    15601 aaggtgcagt taaagtagaa gcagatgcag aaggttggga aactttcgtg attcctgatg
    15661 acatcggcgg acgtttctct gtattaactg ctgttggttt gttaccaatc gctgttagtg
    15721 gtgcagatat cgatgcattg atgcaaggtg ccgctgatgc aagcaaagca tactcaagcg
    15781 acaaactaga agaaaacgaa gcttaccaat acgcagcaat gcgcaacatt ctttaccgta
    15841 aaggcaaagt aactgagttg ttgatcaact atgaaccagg aatgcaatac ttctcagaat
    15901 ggtggaaaca attgtatggt gaatctgaag gaaaagacca aaaaggcatc tatccttcaa
    15961 gcgcaaactt ctcaactgat ttgcactctt taggacaatt catccaagaa ggacgtcgca
    16021 atatttttga aacagttgtg aaggttgaaa aacctcgtaa atcaatcaca attcctgaac
    16081 aagcagaaga tttagacggt ctaggctact tgcaaggaaa agaagtggac ttcgtaaata
    16141 caaaagcatt tgaaggaaca ttgcttgctc atacagacgg tgacgtgcct aacttgctag
    16201 tcaaaattcc agaaatggat gcttatacat tgggttatac aatgtacttc tttgaaattg
    16261 ctgtaggtat ttcaggttac ttgaatggaa tcaacccatt cgaccaacca ggtgtagaag
    16321 cttacaagaa aaacatgttc gcacttctag gcaaaccagg ctttgaagat cttgctaaag
    16381 aattgaacga acgcctgtaa gataagggtt tttcgctaga tttaaataat cagtgataga
    16441 aacatgttcg ctttaaaatg aaaactggta cccatttagg tactcaaaca tactgcatcg
    16501 tttattcaga ataaggggct gtgacaagac gtttgtcaca gtcccttgtt tgtatagaaa
    16561 tcgattgatt gccttgctct ttagtttgtt ttaggacttt aagaacagtg aggctgactt
    16621 cgaaggttaa ttctgttttt ttgttttcta gaatcagtaa gcaaaaaagc actcacttcg
    16681 agtgcttttt tgcgatatat tcttagctta agataggcct aatttttctt ttacagatga
    16741 gggtacctta tcgctggtaa ttactttttt ctctatgatc cgcttttcgc ttgctttcaa
    16801 ctgcaagatc gttccttcgg ggtaagcaga accattggca gagtcaccag aagtaatgac
    16861 atcgaaatct aactgtcttt tttcaccgtt ttcagcataa gcatctacgt tgtatttaaa
    16921 acctcttcca attacttccc cattatctgc tttgatatca gtttctgcag gcattggagc
    16981 tttgactacc gcataataat ctttacctac ataagtagac tgatagtagt cccaaccttt
    17041 ccatcctata aaagcaatca atatgataac gatcaaccct aaaatttttt tcatgaaaga
    17101 tctccttttt tatttttatt a
//
LOCUS       DAJSBL010000058        16607 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.060, whole genome shotgun sequence.
ACCESSION   DAJSBL010000058 DAJSBL010000000
VERSION     DAJSBL010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16607
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.060"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(83..1819)
                     /locus_tag="ONT85_002244"
     CDS             complement(83..1819)
                     /locus_tag="ONT85_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HCR2379197.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSGFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(1816..2520)
                     /locus_tag="ONT85_002245"
     CDS             complement(1816..2520)
                     /locus_tag="ONT85_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR2379198.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            2651..3154
                     /locus_tag="ONT85_002246"
     CDS             2651..3154
                     /locus_tag="ONT85_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HCR2379199.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            3114..3452
                     /locus_tag="ONT85_002247"
     CDS             3114..3452
                     /locus_tag="ONT85_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HCR2379200.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            3473..4891
                     /gene="ffh"
                     /locus_tag="ONT85_002248"
     CDS             3473..4891
                     /gene="ffh"
                     /locus_tag="ONT85_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HCR2379201.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            5003..5581
                     /locus_tag="ONT85_002249"
     CDS             5003..5581
                     /locus_tag="ONT85_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HCR2379202.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            5585..6106
                     /locus_tag="ONT85_002250"
     CDS             5585..6106
                     /locus_tag="ONT85_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HCR2379203.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(6185..6739)
                     /locus_tag="ONT85_002251"
     CDS             complement(6185..6739)
                     /locus_tag="ONT85_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HCR2379204.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            6992..7267
                     /gene="rpsP"
                     /locus_tag="ONT85_002252"
     CDS             6992..7267
                     /gene="rpsP"
                     /locus_tag="ONT85_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HCR2379205.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            7279..7530
                     /locus_tag="ONT85_002253"
     CDS             7279..7530
                     /locus_tag="ONT85_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HCR2379206.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            7655..8446
                     /locus_tag="ONT85_002254"
     CDS             7655..8446
                     /locus_tag="ONT85_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2379207.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            8616..9137
                     /gene="rimM"
                     /locus_tag="ONT85_002255"
     CDS             8616..9137
                     /gene="rimM"
                     /locus_tag="ONT85_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HCR2379208.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            9127..9888
                     /gene="trmD"
                     /locus_tag="ONT85_002256"
     CDS             9127..9888
                     /gene="trmD"
                     /locus_tag="ONT85_002256"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HCR2379209.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            10024..10371
                     /gene="rplS"
                     /locus_tag="ONT85_002257"
     CDS             10024..10371
                     /gene="rplS"
                     /locus_tag="ONT85_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HCR2379210.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            complement(10503..11639)
                     /locus_tag="ONT85_002258"
     CDS             complement(10503..11639)
                     /locus_tag="ONT85_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR2379211.1"
                     /translation="MWIETKTDKNGKKVYKYNERYIDPKTRKRKKVSITYKNKSRETQ
                     KVALLELNKKIDIKLNEKTLQKPDLTFHELVEEWLVIYKRQVKESTYYPTNNILNTIK
                     KKIPETYIVSGINTVDLNNIFEDMIYKDDLSNKYVSVIKSKLNLLFSYAMKKGYVEKN
                     PINEVVIDYKRESKTIKIKDKFLEDDEYKRLVNFTTSHNKRYSLLFQWLYLTGMRPGE
                     AIALSKDDVHITNNNASVVINGTMMYRERSIADMKKSDSTKTAAGMREIDLPKKAIAI
                     YNELLELNPNGQFLFQTTKGTPFQLTAINTYLRNHKADMKIDKKLSSHIFRHTHISKL
                     AELGTPLYAIQDRVGHENSDITEKIYLHVTKGVKEKLKEDIEKL"
     gene            complement(11755..12669)
                     /locus_tag="ONT85_002259"
     CDS             complement(11755..12669)
                     /locus_tag="ONT85_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HCR2379212.1"
                     /translation="MKKIVFGLLFLGLVLSGCSTSPNSASTKENTVSSTSEYETKKLT
                     SSDVHKIKVGDDLTQVYQTLGSPLRKWTNDFVYKELDNAITRDELIVSISKDAQGDYY
                     KEHSELIEHGNSAKDIKKLEMLQYTYSTDSDDETMLIWVSPKTEQVVYYNQRTFLDKD
                     GKSKDSSSTTTTTTTSSSEPQTASVAHSIGEAVPFTSENGNSINVTVQSVSKDYGDDF
                     YKPEGLFYAKVDFLVENTGTTPFDASSSYLEFYDSSDIKSDLASRDYFSETIQPGKSA
                     QGTAYFEVKNDGTTFEVFFADTSWKGSY"
     gene            complement(12760..13188)
                     /locus_tag="ONT85_002260"
     CDS             complement(12760..13188)
                     /locus_tag="ONT85_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HCR2379213.1"
                     /translation="MEMDTINLVEELKRKYQSANPFYICEKMGIKIQYVPFIENPKGQ
                     FQEIRGRAIIFLNDELRDSEERFYICAHELGHAIFHRGLSSYYVSTRTSRSKSESEAN
                     CFAANLIVSLYKEDNDQYPKRIEELKNLYGLPESAYRFLI"
     gene            complement(13193..13567)
                     /locus_tag="ONT85_002261"
     CDS             complement(13193..13567)
                     /locus_tag="ONT85_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR2379214.1"
                     /translation="MNLLDRIKELAHKRGISITQLEEELNIPKNTIYQWKNRTPSTKR
                     LQLVADYFNVTTDYLLGRNQVPDWATKDEVVELDKLLDSNVNMSYGGETLTPEQVQRV
                     KDILIGTFWDIVKEDKEKGKKM"
     gene            13853..14038
                     /locus_tag="ONT85_002262"
     CDS             13853..14038
                     /locus_tag="ONT85_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR2379215.1"
                     /translation="MSENLDLKIRAEMRKRRMTFKELAALIGISGAYLSDILNGNRDG
                     KKAQQHIETVKKILDIR"
     gene            14050..14298
                     /locus_tag="ONT85_002263"
     CDS             14050..14298
                     /locus_tag="ONT85_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2379216.1"
                     /translation="MTKLKKQDFVKKYNYSPSTYQRRMSELKNTAIFSAAYERVTGQE
                     VWINTELYDKFLSFKSYNRLRTRKVTPKEFIEKHLVDL"
     gene            14329..14508
                     /locus_tag="ONT85_002264"
     CDS             14329..14508
                     /locus_tag="ONT85_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2379217.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(14551..15021)
                     /locus_tag="ONT85_002265"
     CDS             complement(14551..15021)
                     /locus_tag="ONT85_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2379218.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            15108..15806
                     /locus_tag="ONT85_002266"
     CDS             15108..15806
                     /locus_tag="ONT85_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HCR2379219.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            15984..16325
                     /locus_tag="ONT85_002267"
     CDS             15984..16325
                     /locus_tag="ONT85_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2379220.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            16318..>16607
                     /locus_tag="ONT85_002268"
     CDS             16318..>16607
                     /locus_tag="ONT85_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HCR2379221.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTET"
BASE COUNT         5465 a         2900 c         3247 g         4995 t
ORIGIN      
        1 agtaaaaaag aacaaccact cgtgtatctt tcgaagcagg atgagtcatc caactttaaa
       61 acgcacgagc agttgctctt ttttacattt tcggaatggt aacggtgaaa gtcgaacctg
      121 ttccaagatg actgtctaca gtaatcgtac cattaaggag ttgcacgtag tcttttacaa
      181 tcgctaaccc taatccggtc ccgcctgaat gtcggcttcg cgccttgtct acgcggtaaa
      241 aacgctcaaa gatccgttcc tgatcctcaa tgtcgatacc tataccaaag tcttggacag
      301 aaaacgatag atcatctgtt gcctgatagc ggataataat ctcgctgtcg ctttttgaat
      361 attggacagc attttcaatt agatttttga taattggata aaggatatct gttttagtag
      421 taaatggttc gttctttcca atcaatcgaa tcgtcaaccg tttcttttcg atgattggct
      481 gataattttg aacgatttgc tggaaataag gttctgtata aattgtctgg atctcgtaag
      541 aattctcgcc atcttttgac agctggatga tttcccgaat cagtttatct aaccggatcg
      601 catctttttg catgatctca agaaaagaag tcagagtttg cgggtcatct tttgcaccat
      661 ccagtaaagt ctcggtaaaa ccaatcagtg aagtcacagg agtttttaat tcgtgagaga
      721 cgttgccaac gaaatccttt tgtagctttt ctaaacgtcg cactttcgtt aagtcatacg
      781 ccacccctag aatctgtccg gtaccttctg aattagtaaa gtagcgcaaa ctcaagtcta
      841 atgtctgccg accttctgta agggtgatct cctgatggat cagcggatgc tcaggagtca
      901 cttgatgaat cagctgaatg agttttggct cttgaatgac ttctgtgtac cgctgttcag
      961 gttgatagct taccacgttt aaatgtagct gcatctttgg attaagcagt agtaaacgag
     1021 aatcagcatc atcaatcagg aatacgccaa tcatcagttc gttcagtaac gtatataatt
     1081 gctcttctgt ggtagtacac gcccgataca tcttactcat ttgttcgctc aacgcattaa
     1141 tcgtctgata caattcttcc cattgatcgg gtgtttgcat gattacttcc gcctgattag
     1201 gattttgcac catttttttt agtacgggca gcacggtttt caatggctgg tttttttgat
     1261 agatcagata atagatcatt cctgcaagaa caaggaagaa agacagaaag aagaaaaaga
     1321 cccaacggcg aaaactctca gttctcggta aaaaaccgct tgtcggttct gcgatacgta
     1381 aaatggcttc taactggcca ctttttttga ctggtaatgc gacatataaa agatcctcat
     1441 tcaaagtagt acttttgcgc aaagcgctac ctaggttccc accattcaat acggctttga
     1501 tttcgggacg ggagtttcgc tgttcattca atgtttcatt gtttgtgtca aataagatct
     1561 ttccagtagc atccattaaa gtaatccgtt cgttcgactg atggacgaat tcttccaaag
     1621 cagttttgtt ttgcgctgct tggaggtttt cgacatctaa ttgccggatc agcagttgtg
     1681 ctttttttgt caggtagctt tcttgctgtt ccacgatctg ctgttgaaaa taatgggaaa
     1741 tcaattgcca gccaccaaaa aacaaactaa ctaaaagaat aagaaagaag agacgttcat
     1801 aaattttttt ccatttcatc gtttgggttc ctgaaactta tagccaaatc ctctaatagt
     1861 caccaagtac tttgggtgtt ttggatctcg ctcgattttt tctcgcaaat gactaacgtg
     1921 tacatctacg attcggcttt gtcctgcgaa gtcaaaattc caaatgcggt ctagcaatgt
     1981 gtctcggtcg atcacacggt cttttcgttt catgaaataa acaagtaatt caaattcttt
     2041 cggtgttagt tcaatcagat ggtcgtctac ggtcacttga taattggtca aatcagcagt
     2101 gatttgtcca atcgatagat attcaggctc agcttcatct tttgattgct tacgaggttc
     2161 aattcgtctg aatattgctt tcatacgggc taatacttcg cgaggactaa atggtttggt
     2221 caaatagtcg tcagcaccta tttctaggcc aatgatccga tccacttggt catctttagc
     2281 cgtcagcatc aaaatcggtg tttcattttt ttcttgacgc agccgctgag tgatctccat
     2341 gccatccatc gttggaagca tcacatctaa aatgatgaaa tcaaatgatt gttctaatgc
     2401 taattctaat ccttcgcctc catctgtggc actggttacc ttgtagcctt ctttttccaa
     2461 attgaacgtc aagagcgtta caatagaagg ttcatcatca acaactagta tttttttcat
     2521 tcaaatctcc tttaaatagg tttcattctg tttatatttt agcatagaat cagtacaaag
     2581 ggctatttta caaaagaaat aaatggtata ataggaaggc actttgctga atagaaagga
     2641 agagcagtca atgattggaa tcagtgcatg tttaggcggt ctggcctgtc gctatgatgg
     2701 acagaaaaaa gaagtaccag aattgatgga actagttgaa actggagaag ctgtcatggt
     2761 ctgtccagaa gtgatcggcg gattaccaat tcctagagat cccgctgaaa tcattggtgg
     2821 aaatggattc gatgtgtgga aagatcaagc aaaagtatgg acgacaagcg gcgaagatgt
     2881 aacggaagcg tacaaaaatg gcgcgatcaa agcctatcaa aaattgcagg aaaaacacat
     2941 tgatctagtc atcatgaagg aaaaaagccc ttcatgcgga acccattcga tttatgacgg
     3001 aagtttttcc ggaataaaaa aaaatggtgc cggagtggct actgcttatt ttattcagca
     3061 aaaaatgact gtgctctctg atgaagaatg gctgaaaact agaggtgagc agaatggaaa
     3121 tcgaaaagac caatcgaatg aacgcgctct ttgaatttta ttcgacctta ttgacagaaa
     3181 aacaaatgaa ctacatggaa ttatattatg cagatgattt ttcattaggt gagatcgcgg
     3241 aagaatacga agtaagccga caagcagtat atgacaatat caagcggaca agcaagatct
     3301 tggaggatta tgaaaaaaaa ctccatcttt tttctaatta tattgttcga gaacaggtat
     3361 tggaaaatat gactgcatat attacagata agtacccgga agataaaaaa ctacttgaat
     3421 atgtacagca aattcaagca atcgaagaat aaataaaagg aatgaagaaa atatggcatt
     3481 tgaaagctta actgaacgtc tccagcaggc gatgggaaaa ataagaaaaa aaggaaaagt
     3541 atcagaagct gacgtaaaag aaatgatgcg ggaaatccgc cttgccttac tagaagccga
     3601 tgttaacttg caagtagtca aagattttac taagcgcgta cgcgaacgtg cggtaggggc
     3661 agaagtgctg gaaagtttat cacctgcaca acaaatcgta aaaatcgttg atgaagaatt
     3721 gacgatcaca cttggttctg aaacagcaga gttaaataaa tcaccaaaaa taccaactgt
     3781 tatcatgatg gctggtcttc aaggggcagg taaaacgacc tttactggta aattagccaa
     3841 ttatttaaag aaaaatgaaa atgctcgtcc gctcctaatc gcaggggacg tatatcgacc
     3901 agctgcgatc gaccaattga aagtattagg tcaacagcta gacgttcctg tatttgatat
     3961 gggaactgaa gtcagcccgg tagagatcgt ccgtcaaggg atggagttag caaaagaaaa
     4021 gaaaaacgac tatgtattga tcgatacggc aggacgtctg catatcgatg aaacattgat
     4081 ggatgagttg aaacaaatca aagaattgac gcaaccaaat gagattttgc ttgttgtcga
     4141 tgccatgact ggtcaagatg ccgtcaacgt agctgatagc ttcaaccagc aattaggtat
     4201 tacaggggtc gtcatcacta aacttgatgg ggatactcga ggcggggcag cactatcgat
     4261 ccgctcagtg acaggcgcac caatcaaatt catcggttct ggtgaaaaat tgactgactt
     4321 agaagtattc cacccagatc gtatggctag ccgtatcctt ggtatggggg atatgctgac
     4381 attgattgaa aaagcgcagc aagactacga cgagaaaaaa gcggaagaac ttgcacagaa
     4441 aatgcgggaa aattcattcg atttcaatga ctttattgaa cagcttgatc aagtgatggg
     4501 tatgggccca ctggaagatc taatcaaaat gatcccagga atgaatcaag tccctggaat
     4561 cgaaaacgtc aaggtagatc caaaagatgt tgaacgcaaa aaagcgatgg tttattctat
     4621 gacgccagca gaaagagcaa atcctgatct gttgaaccca agccgtcgtc gccgaattgc
     4681 tgccggttct ggaaacagtg ttgttgaagt gaatcgaatg atcaaacaat tcaaggaatc
     4741 aagaaaaatg atgcagcaaa tgtccaaagg ggatatgaac atcccaggta tggaccaaat
     4801 gtttggttca ggcgtcaaag gaaaactagg caaaatggcg atgaatcgaa tgatcaagaa
     4861 aaataagaag aaaaagaaaa aacggaaata agatgaaaaa aacagcttcg gtacagactg
     4921 aaaatgtcta ctgccggagc ttttctatca gcaattttta ttctttacta atacaattac
     4981 tctaaacgaa gaggtggcac agatgagatg tccgtggagt gaacaaacag aaagtatgcg
     5041 gcaatatcat gatactgtct ggggtgttcc tgaatatgat gaccaaaagc tgttccgaaa
     5101 attgatgctt gatattaatc aagccggatt gagctggcag acgattttaa ataaaagtga
     5161 agcattcgat gaagcatatg atcgattcga tatcaaaaaa gtcgcaaatt atggagaaac
     5221 aaaaatcagt gaactaatgg aaaacaaagg aattattcgt aaccgaagaa aaatcgaagc
     5281 agccgtccaa aacgccaaag cagtcttgaa aattcaagaa gaaatcggca gtttttcaga
     5341 gtatctttgg tcgttttcta atggaaaaat cgttgattct tcttatcatt cacaagaaga
     5401 agtcccaaca acaagtcctc tttcagatga attagcaaaa gatctaaaga aaagaggatt
     5461 taaatttatc ggaagcacaa caatctatgc ttttttagaa gctgtcggga tcatcaatga
     5521 tcaccttgac agttgtttcc gcaaagaaga ggtcgtacgt atcggcagaa agggcgaata
     5581 aagcatgcga aaaatcatat tttatggtgc tataagttta gatggctatt tggcaacgaa
     5641 aacggatgat cttcaatggc tttttgacac tccaacaggt aagcaaacta cttatgaagc
     5701 tttttatgaa acaatcgata cgaccatcat gggcagaaaa acttatcagg aagcaaaaaa
     5761 atatttggag accgaagcaa tttatccaga gaaaaagaat ttcgtttttt ccacccacgc
     5821 acaattacaa ttgccagatg ctgaggtcgt aaacgaagat cctgttgctt ttctaaaaaa
     5881 tctacaaatg acagaaggaa aagatatctg ggttgtgggt ggcggtcgat tgatcaaacc
     5941 gctattggaa catcaaatga ttgatgaatg gtatatccag attacccctg tattattagg
     6001 ggacgggatt cctttattcc aaaaaggaac atatgaagcg cgttttgaaa tgctagacac
     6061 gacacgattc ggtgaattta tcgaattgca ttatgtacga aagtagaagt aaataaaata
     6121 aaaaaggcaa ctatatgcag aaatgtcctg tacatagttg ccttttttat taccgttatg
     6181 ctttttactc tgttaatttg ttagaaaaat ctagatacgc atctaaaatc gtttggaaga
     6241 aacttaatgt tccttcaacg atattaccat tttcatcaat tagattcgca acgtttccga
     6301 tatatgcttc cggttgctgc aaggttggca cattcaaaaa gacaagtgat tggcgtagat
     6361 ggtggtttgc gccaaagccg ccgatcccac ctggtgatgc agtaacgact aaacctggtt
     6421 tatgatccca aacactgtgt ccataaggtc ttgagcctac atctaaagca tttttcaata
     6481 ctgctggcac agaacggttg tattcaggag taacgaaaac gacaccgtct agtcccctca
     6541 cttcctcacg aaaacgagtc cattcagcag gagcctgttc tggagtttcg agatcctcat
     6601 tgtaaaatgg cagatcatcg atttttacaa atacactttc gtacccttca ggaagcaatt
     6661 tcgcaaaagc ttctgctact tttttgttgt aagaatcttt tcttaaacta ccaataaaaa
     6721 atccaatttt ttttgtcata cgatttttcc tccttcaaac tatctataat aatcttacaa
     6781 aagataagta actggagcaa ataaaaacac ttactgacaa aagctacata gaagatagat
     6841 tacttatttt taacaaaaaa gaagtctaaa atcttctatt ttctaaaaaa aatcgaattt
     6901 aatcaagctg taaagaaaaa aagctttaca gggtaaaaaa aaactgttac actacatctt
     6961 gtgaataaaa caatggaggt ggaaataaat aatggcagtt aaaatccgtt taaaacgtat
     7021 gggttctaaa aagagtccgt tttaccgtat cgtcgtagct gattcacgtt ctcctcgtga
     7081 tggacgtttc atcgaaactg taggtactta caatcctttg aaagaccctg cagaagtagt
     7141 tttaaaagaa gacttagttc ttgactggtt atcaaaaggt gcacaacctt ctgacactgt
     7201 acgtaacatc ctttcaaaag aaggcgttat gcaaaaacac cacgaagcta aattctcaaa
     7261 gaaataaggt gaccggatat gaaagattta agcgacttag tcttaacaat cgttcgtccg
     7321 ctagtcactt atcctgatca agtcaagtta gacgtcgtag agtcgaaaga cttttacgag
     7381 tacaatctaa cagtggctcc tgaagatatt ggccgtatca ttggaaaaca agggcgtgtt
     7441 gctaaagcaa tccgcacaat cgtttacggt gttcggatca atgcaccgaa aaaagtgcgt
     7501 ttgaatatca tcgatgataa ggaaaagtaa ataaacgtgc tgtactcgaa ttttcgagtg
     7561 cagctttttt gtttttttta ttaattgaag aaatgattat ttggcaaaac aagcaagaaa
     7621 gcgtatcctt ataggaggag ggagtgaaca gccaatgaga aagaaaatgt tatttattcc
     7681 atttgctcta ttgttaagcg cttgtagttc ggttactcaa gaatcagaca atacacatct
     7741 atcaagcagt atatctgaaa ctgttaccag tccagaaaaa agtagtacca aaacggcttc
     7801 caccactgaa caaacaatgg ctacctcaaa taatgagaaa aagactgctt tagaccagtt
     7861 gaaagaacaa cagccaaatg ttcccatgcc tctagatgta ccagtctctt caggctatct
     7921 caatatcgca gcgacacata caaagcaagg ttattctatt ttatattaca gaacagatcg
     7981 tcccctcggg ttgaatgctg acgaattgaa tcaagaaacg cctattgcaa cctatctata
     8041 tcaatatggg tttgcttcta gtcaagaaac aatccaagtt ttgcagccat ttgagatcga
     8101 cacaaatggt cagcaagtgg acttaggttc tcgaatcaca gggtatcagc aaggtgcagc
     8161 aggttcaagt tttcttgaat ggcaagaagg aaactggcgt attcgaatcc gtgggaataa
     8221 tatagaaggc caagatccct tactattagc taaagaaatc gtagcttatt tggaagaaaa
     8281 cagtctacca gcacctgaac aatttggtaa aattactgtt gatatgggtg atacgacgaa
     8341 tcgagccgta gaagtaagct ggcaagaacc gaaaaatgcc tatacgatca ctcatcaaga
     8401 tcctatgtct gcgttgaaga tggcagtttc tatgaagagg ttgtgaaatc agcgcgctga
     8461 tctgagccca tcatttaact ccaagtcaca ggaagtaatc aaaaatagca cgccgcattt
     8521 ttgcataatt ttggcttaat accttaaaag aaaactagaa ttgcctcttc acacatgcta
     8581 aaataaaaca gacaaaaaag aaatggggat ataagttgac tgaatacttg aatgtaggaa
     8641 aaatcgtaaa cacacaaggt atcaaaggcg aagtccgtgt tatctcaaca acggatttcc
     8701 cagaagaacg ttacaaaaaa ggaacggtgc taacactatt ccaagacgga aaagcacctg
     8761 tggaattaac agtcaaaagt catcgcaaac ataaaaattt tgacttattg agttttgaag
     8821 gacatccttc tatcaatgat gtggaaaaat atcgggatgg catattaaaa gtgtcaaaag
     8881 acaactctgt agatctagct gaaaatgaat tctattacca tgagatcatc ggtctgcatg
     8941 tattagaaga tggaactgag ttaggtaaag taaaagaaat cttgtcacca ggtgccaatg
     9001 atgtttgggt cgttcaacgt ccgaaaaaag ctgatatatt gcttccatat atcgcttctg
     9061 tcgtcaaaga agtcgattta gaagctggag tggtccgtgt agaaattcca gaaggactga
     9121 ttgacgatga agattgatgt attgacttta tttccaagaa tgtttgaagg tcctatgggg
     9181 gaatccatca tcggtaaggc tgtagataaa ggattattag atatcaatat ctctaacttc
     9241 cgcgattatt cagataacaa acatcagaca gtagatgatt atccatacgg cggtggagca
     9301 ggtatgctgc tcaaagtcca gcccatctat gataacatcc aagccattga aaaagctgca
     9361 ccggatgtca aaaaaagagt aattcttctt gacccagctg gtaaacgatt cgatcagaaa
     9421 atggcagaag aattttcaca agaagaacac ttagtattca tttgtggtca ttatgaggga
     9481 tatgatgaac gtatccgcac attagtgaca gatgaagttt ccttaggaga ttatgtcctt
     9541 actgggggag aattaggtgc gatggtcatg atcgatgcaa cagttcggct tttgccagat
     9601 gtgttaggga atcagacttc agctcaaacg gattcttatt caaccggttt attggaacat
     9661 ccccaatata ctcgcccggc agaatataaa gggatgaaag taccagaagt attgacaaat
     9721 ggaaatcata aattgattga agaatggcaa ttaaaagaat cactaagaag gacctatcaa
     9781 cgtcggcctg atcttttgga aacacttgaa tggacacctc aaatgacgaa atttttagaa
     9841 gaaatcaaga aagaagaaca agaaaaaaca gcagatgaag ccaagtgact ttttctaaaa
     9901 tcttctttac agtaggttga taatatgtta gtattttatg gtgagtgcaa aagcacttaa
     9961 ctattacgat attccgctgt gagaaaagct cataagaata tttggaataa ggagaagaaa
    10021 accatgaatc cattaatcga agaattaaca aaagaacaat tacgttctga catcccagcg
    10081 ttccgccctg gtgacactgt gcgtgttcac gcaaaagttg ttgaaggtac tcgtgaacgt
    10141 atccagttat ttgaaggtgt tgttatcaaa cgccgcggtg ctggaatcag cgaaacttat
    10201 acagtacgta aagtttctaa cggagtgggc gttgaacgta cattcccatt gcatacacca
    10261 cgtgttgcaa aaatcgaagt agttcgctac ggtaaagtac gtcgtgcaaa attgtactac
    10321 ctacgtgcat tacacggaaa agctgctcgt atcaaagaaa tccgtcgtta atcatcgtta
    10381 tcagactgag aaagaaccct tggtattcaa gggttctttt tttgttctct gtttttttat
    10441 attaacgatt tctatttggg acatttttga ttaataaaaa ggggcaaaaa aggggcaaca
    10501 agttacagtt tttctatatc ttctttcaat ttttctttta ctccctttgt cacatgcaaa
    10561 taaatctttt cagtgatatc actattttcg tgcccaacgc gatcctgaat agcatacaga
    10621 ggagttccta gttccgctaa ttttgaaata tgggtatgtc taaaaatatg tgaactaagt
    10681 tttttatcaa tcttcatgtc agctttatga tttctcaaat aggtgttaat tgctgttagt
    10741 tggaaaggag ttcctttcgt tgtttgaaat agaaattgac cattcggatt taattctaga
    10801 agctcattgt aaatagctat cgcttttttt ggtaaatcaa tttctcgcat tccagcagca
    10861 gtttttgtag aatcagattt tttcatatca gctattgaac gttctctata catcatcgtc
    10921 ccatttataa ctactgatgc attattattg gtaatgtgta catcgtcttt acttaatgcg
    10981 attgcttctc caggcctcat tccagtcaaa tatagccact ggaaaaggag agaatatctt
    11041 ttattgtgtg aagttgtgaa atttactagt cttttatatt catcatcttc taaaaactta
    11101 tctttaattt ttatcgtttt tgactctcgt ttataatcaa taactacttc gttgatagga
    11161 tttttttcta catagccttt tttcatagcg tatgaaaaaa gaagattcaa tttggattta
    11221 attacactga cgtacttatt tgacaagtca tctttatata tcatatcttc aaaaatatta
    11281 tttaaatcta ctgtattaat accagaaacg atgtaagtct caggtatctt tttctttata
    11341 gtgtttaaga tattattcgt aggataatat gtagactcct taacttgtct tttgtaaata
    11401 actaaccatt cttcgacaag ctcatgaaat gttagatcag gcttctgaag tgttttttcg
    11461 tttaatttta tatcaatttt tttatttaac tctagcaatg ccactttctg agtttctcga
    11521 gatttatttt tataagtaat gctcactttt tttctttttc tagtttttgg atcaatatat
    11581 cgctcattat atttatatac tttttttccg tttttatcag ttttggtttc aatccacatt
    11641 gttttcatct cctatttgtt gctataatag gaatggataa gtaagccaat tatagcaggt
    11701 ttatttttca tcacgtccac aaactttggc gagggagggg gcgtgttttt ttgtttagta
    11761 gctacctttc catgaagtat ctgcgaagaa cacttcaaat gttgttccat catttttcac
    11821 ttcaaaatat gctgttcctt gtgcggattt acccggttgg atggtttccg aaaagtaatc
    11881 tctactagct aaatcagatt taatatctga tgaatcataa aattctaaat aacttgaact
    11941 tgcatcaaaa ggtgtagtcc ctgtgttttc tacaagaaaa tctacctttg cgtaaaatag
    12001 gccttctggt ttatagaaat catcaccgta atcttttgat acagactgga cagttacgtt
    12061 aatagagtta ccattttcag aagtaaaagg aactgcttct cctatactat gtgcaactga
    12121 tgccgtctgg ggttctgaag acgaagttgt agttgtagtt gtagtagagc ttgaatcttt
    12181 ggattttcca tctttatcta agaaagttcg ttgattataa tatacaacct gttcagtttt
    12241 tggactaacc caaattaaca ttgtttcatc atcagaatca gttgaataag tatattggag
    12301 catctctaat ttttttatat cttttgcact atttccatgt tcaattaatt cagagtgctc
    12361 tttataataa tctccttgtg catcctttga aatagataca attaattcat cccgtgtgat
    12421 agcattgtct aattctttgt aaacaaaatc atttgtccat ttacgtaacg gtgaaccaag
    12481 tgtctgataa acttgagtta aatcatcacc aacttttatt ttatgaacgt ctgatgaagt
    12541 tagttttttt gtttcatatt cagatgttga tgaaacagta ttttctttag tgctagcaga
    12601 attaggacta gtggagcatc cgcttaatac caagcctaaa aaaagcaacc caaaaacaat
    12661 ttttttcatt tttaattcct cacttctatg atatgatttt tttgtaggat cttagaaata
    12721 aggtttttag tccgtgttgc agcacggact ttttttacat tatataagaa agcgataagc
    12781 gctttctgga agtccgtaaa gattctttaa ttcctcaatt cgtttaggat attgatcatt
    12841 gtcttctttg tatagagaaa caatgagatt agcagcaaag caattagctt cgctttcaga
    12901 tttgcttcta gatgttcttg ttgatacata gtaactggat aagccacgat gaaaaatagc
    12961 gtgacctaat tcgtgagcgc aaatgtagaa tctttcctca gagtctctca gttcatcatt
    13021 taagaagatt attgcacgac ctctaatttc ttgaaactgt cctttgggat tctcgataaa
    13081 aggaacgtat tgaattttaa tgcccatctt ttcacaaata taaaaaggat tagcggactg
    13141 gtatttccgc ttcaactcct cgactaaatt aatcgtatcc atctccataa gctcacatct
    13201 ttttgccttt ttctttgtct tctttcacaa tatcccagaa ggttccaata aggatatctt
    13261 ttacgcgttg gacctgttca ggcgtcagtg tttctccgcc ataagacata ttgacatttg
    13321 aatctagtaa tttatcaagt tcaactactt catcttttgt tgcccagtca ggaacttggt
    13381 ttctaccgag taaataatca gtagttacat taaaataatc tgctacaagt tgtagtcttt
    13441 tagtactagg agttctattt ttccattgat aaatcgtgtt tttaggtata tttaattctt
    13501 cttccaattg agttatactt atgcctcttt tatgagctaa ttcctttatt ctatctagta
    13561 aattcatttt cgcacctcac aagctacgaa aacactaata aaaaaattag ttaaaggtgt
    13621 tgacaactaa taaaagtgtt agtatactgt tttcgtaagc taaaatatta gctaaaaaga
    13681 cttgataact aataaacact tcacggtcgg caaactctga aatgtaaatt actaggcatt
    13741 tctgtgtctt atttagctat gtctatatac taataaaaat attagttatt gtcaacgaat
    13801 attagctaat tttttagctt acagattatt tttttagaaa ggagctattt ttatgtctga
    13861 gaatttagac ttaaaaattc gagcggagat gagaaaaaga agaatgactt tcaaagaact
    13921 agctgcgctt atcggtattt caggagctta tttatcagat attctaaacg gcaatcgtga
    13981 tggaaagaaa gcacaacagc atatcgaaac agtgaaaaaa atattggaca tccgataggg
    14041 gggtaggcga tgacaaaact aaaaaaacaa gattttgtaa aaaaatacaa ttattctcca
    14101 tctacttatc aacgtcgaat gtcggaacta aaaaatacag caattttctc agcggcgtat
    14161 gaacgggtca caggacaaga agtttggatc aatacagaat tatacgataa atttttgtct
    14221 ttcaaatcct ataacaggtt acgcacaaga aaggtaacac ctaaagaatt tatcgagaag
    14281 catttagttg atttataaaa aacagaacat tttgagaggt gaaggttaat gggtaaattc
    14341 aacagagcat tggtattcag cgcaccgcta atcatctacg ctttaggact ttggggaagc
    14401 aggcaagcgt tgataggaac gattgtttac atggtttgga tttttatggg gcttgatgaa
    14461 gctgaagctg agtacagagc gaaaaagcca accgaggggg ctgactaaaa atgaatagaa
    14521 aagagaaact agaatgtata ttactattac tcagtttaat tctttcacta atttctctat
    14581 tgggaagttt gtatttttga tatcaacaat tttgtggatt tgggttctat ttagagtaaa
    14641 tatcattttt gtttggtgat taaggagttt attaaaatta attttcccta cttcaaatgc
    14701 aaggtaacaa gatatagcac ttttaggagg aatatttagc ggtacagctt cagagtatat
    14761 tctattatca tcgactgatc ctcctttagc aattaatttc ttaaatggag tagctgacca
    14821 tctgcggttt aagttagcgg atgaaagttc taaattaact aacatagctg gttctgttga
    14881 atagttagaa atgattactt ttgtataaat catatctgca gcgaagtaag aagcattaag
    14941 ttcaacttct agttgagggc gcattccttt tatttttatc cctgttaaaa acgtactcaa
    15001 tataaaactt attattgaca tccattgaaa aattgttaag tgaaaaaaat tcaaaataaa
    15061 caccaccagt ttttaactaa attataccaa aaaggagaga agaaataatg caagaattag
    15121 taattttgaa aaataaagaa gctgtgacta cgagcttaca agtggcagaa agcttcgaga
    15181 aaaaacatca acatgtttta agagatattg atgcactaaa aaaagatgtg tccaattttg
    15241 gacagatgtt tgtagaaggt aatgaaccag attcatatgg cagaaatcga cgagttttct
    15301 tcattagtag agatggattt ttcttgctgg ctatggggtt tacaggaaag aaagctatct
    15361 acttcaaaca aaaatacatt gaagcattca acgaaatgga agatgttatt cgcaagaata
    15421 ctgttcctca aacaattgaa gatatgatga tctatcaatt agaagaaatg aaagatgtta
    15481 aaaaagatgt ttccatgctt aaagatacta tgcgaattag cggacaacaa gagtttgaaa
    15541 ttaagcaaaa aggaaatatg aaagttatgg gagttctagg gggaaaagaa agccgagctt
    15601 atgaagaaat cagcaaaaaa gtattctcaa aattttggtc tgaatttaaa cgtacctttt
    15661 caatcccaag atatggcgag ttacctcgta agagattcga tgatgctgtt tcatttattg
    15721 aaatgtggtt gccagaaact gcgatccgta tggaaattga tcaactgaac agacaacaga
    15781 gactttttgg tgatgacaat gaatagagct gaagcgctaa gaatagggac ggcaattgct
    15841 aattgctggt ggaaatacta taaaccaatc atcctaagcc aacaacatat tgacaagcaa
    15901 aaagcatggc aacaaataaa aaagtgactc cgccggcaag caaagagtca caaagaaaac
    15961 acatcataag gagattttag catatggaaa aagaactttc cactctagat caatatttga
    16021 ttgatcctga ttggggcaag ccgaaaattg aggaaacaag tggtcgaaaa atcagacgaa
    16081 atcttttgac gaatgaagaa ctagcttggg atcaagatga tttaggcaac catgtaacta
    16141 tttgggatca tgtttatctt atccatctat cgaagcattc gaataaacct gaatatattt
    16201 acgtcatcga agatggcttg attgatgcgc tagaagagta cgacagagat aacttgattg
    16261 atatctctta ttacggacca ggtaagaaat acattgctga aatggaggca gaatttgatg
    16321 agtgaaatca aagggacaac gaactttgaa aaacttttta gtcgtaagtt aaataaaatt
    16381 ctcaagaaaa aaggaaattt tgattattta tcttgggctc acgcgtggga gattatgaaa
    16441 aagaatgatc cacaggcaac ggtaactatt aatgagtaca aacactacag ggttgtttct
    16501 ggaactcatc aagactttct tgttgaggaa tataaacctt ttcttatgga tgaaactggg
    16561 acttatgtat ctgtctcagt aacggttaaa ggacacacgg aaaccga
//
LOCUS       DAJSBL010000059        16130 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.061, whole genome shotgun sequence.
ACCESSION   DAJSBL010000059 DAJSBL010000000
VERSION     DAJSBL010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16130
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.061"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..1228
                     /locus_tag="ONT85_002269"
     CDS             <1..1228
                     /locus_tag="ONT85_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HCR2379222.1"
                     /translation="FFDEEWLIEKEYKGVQAQFIKGKLDDSPSYCEHCRSVRIIRNGS
                     YTTRTQILKVKEKLTILELKRTRFLCHDCGRTFSAKTDLVDEHHQLTKELKQAILMEL
                     YENQSRKLIAKKYFVSDGTVTRILREATKHYQSRMNFLPTVLCMDEFKSMKSVSGSMS
                     FICVDGTTHQLFTILEDRRLYKLTQYFLHFPRKARLKVKYLVMDMNASYCQLLKTVFP
                     NAEIVTDRFHIVQHINRSFNQLRVQIMNRFRTSHSENQKKYRRLKRYWKLLLKDSTTW
                     EPLKRHYHRLFKRPISQTEIVDELLSYNEELRTAYHFCQLLRYYFVKRGTEGFQETLK
                     TISSTLPQSFKKKFTIFHCYQSGISNAFKVSHSNGVTEGLNNKIKLIKRIAFGYRNFY
                     NFRARIYLQQGLIFEN"
     gene            complement(1611..2339)
                     /locus_tag="ONT85_002270"
     CDS             complement(1611..2339)
                     /locus_tag="ONT85_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HCR2379223.1"
                     /translation="MLRIAIVDDDCTIVEGIRHYLLKKVPDIIIDHCYTDGDEAFKNL
                     QNQRFDLLITDITMGKISGLELIESLQQTSNRYFTSIVISAHQNFQYAKKGIDLSIKS
                     YITKPIDFPYLVKLVQEEKMRKSAANQNSLTDDSFNESIQQVIDYVQNNYSEKLTLKD
                     VATQFHYHPAYLGRAFKKETGYSFHDYLLDFRIKRASALLKDTFLSIDEISQKVGIDH
                     VSYFSVLFKKTTGHSPSNYRKKLL"
     gene            complement(2317..3891)
                     /locus_tag="ONT85_002271"
     CDS             complement(2317..3891)
                     /locus_tag="ONT85_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HCR2379224.1"
                     /translation="MKNDLSKVIMRFTYILIFFITLLACFTLFFSYINVIEKEKNQNL
                     QNIQQITHYMETYNRILSGIVTEIYQNYDFQENLNDYYLSSPAEYMEDILSKDRSPTY
                     LVDNLKKLVQDDEVKNISISLDYEKYLSAYAPTKNEKISFIRSLSFISEDMPDATLIL
                     DVSINALKKSLFPTHYNYSLSNGIDSVNLSEDISPSKWKKTQQIAIGNHTLQVYENPN
                     YSSQTLLGLAIMIFLLMSLFILAIHQLLNRSFRNYIQQYQEIITSLNHSSTGDYKSIN
                     IENKVGDLKEIAMHINQFYLDNATYQKTLYENNWLKQEAKYAALQYQIEPHFLFNNLE
                     FIRMTAILNDDEEISDSIYLLSKLYRNNTYQPDIIDLKEELINIDLSLQLHQRRYRHN
                     FDYQLIDHIDQLMLPKLSLQPFVENYIKHGFDSSKKENRLTVYATETTNHYVLKIVEN
                     GKGMDQDKLEHIQQKLNDDIPYSENIGISNSFRRIKHLYPGDTRFVIESQLHVGTTIT
                     IKIQKGGNMHVENCNC"
     gene            4120..5058
                     /locus_tag="ONT85_002272"
     CDS             4120..5058
                     /locus_tag="ONT85_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCR2379225.1"
                     /translation="MKLKKGLLGNLKKNRALFIMLLPGALWLFIFCYIPMFGVVIAFK
                     DFRISSRGFFTSLFNSPWVGWENFKYLFASQDAWIITRNTVVYNVVGILLSTFCTVSL
                     AIILSQMANKRLLKKMQTFMIFPNFLSWVVISYFVYAFLSTDKGLINILLSNFGVEPI
                     NWYSEPKYWPFIIVFTSLWKGVGYGSIIYYASIMGIDTSYYEAALVDGASKWQQIRNV
                     VLPHLRSIIVIMLILSVGSIFRADFGLFYQLPRNSGALYDVTNVLDTYVYRGLMNSGD
                     IGMSTAAGLYQSSVGFVLILITNKIVNKLDPSSALF"
     gene            5070..5981
                     /locus_tag="ONT85_002273"
     CDS             5070..5981
                     /locus_tag="ONT85_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCR2379226.1"
                     /translation="MISKLKRQMYFSLPVERFFTILLILFTLSCIFPFIFVIVISLTD
                     EKALQLNGYELLPAQWSTDAYQYLIQDGGQLLRSLGVTIMITVIGTLITVFMTGTYAY
                     VLSRASFPYRKFFTFYLFFTMLFAGGMVPSYLVMTKMLGLKNTIWALILPLAFSPYNV
                     IILRTFFKKSIPESIIESAKMDGCSEFRVFFQIVLPLAIPGVATIGLFSSLGYWNDWF
                     NALLYIDTNKLIPLQYLLMKIQNSMEFLANNNDITLAQQQAIQNSLPQESTRMAMVVV
                     ATLPIAIVYPFFQKYFVQGLTIGGVKE"
     gene            6005..7477
                     /locus_tag="ONT85_002274"
     CDS             6005..7477
                     /locus_tag="ONT85_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HCR2379227.1"
                     /translation="MKKNMQKYLIGGASVLTLGLLGACGSSNGEANNETGTTKVTWYQ
                     VGEEPKDFDEVMTKVNEKLKEEINVELDMRLIPDGDYQQKLGVMINSGEEYDICFVNG
                     TDYVNYGNKGAFISLNDHLDKELKDYAAELNEGFIEGGAINGETYGLPVNNDIATTEY
                     WTFNKDLVDQYQLDVENVEGVETLAPVLEKFAEKNTDTNITPIGMTRTSVCIPYDNII
                     GGGVPLAVAFEGDTQTVVNTYETPEMMTYLKAMHEFYKKGYILKDAATVTTDPFRIDS
                     KNWFLGRNSSGYQPLAQKKLEALAGHGLILKPAGKAFKTTGFARVAMHAVSASSKHPE
                     AALKLLNYVNTNQEVADLMVYGIEGKHYDRNEDGSITLTNSDDYYQGAWALVNTYLVS
                     KTSPITEESKKLEEESKKFQNEAPMSPILGFSFDASDFRTEIASVQNIVSQYEVLLSS
                     GTVDPEQYVPELLEKLEKAGLDKIQASAQEQYDEWRKSNK"
     gene            7528..8139
                     /locus_tag="ONT85_002275"
     CDS             7528..8139
                     /locus_tag="ONT85_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HCR2379228.1"
                     /translation="MNENKWTKGFNWVFQLLLLNFTYFILICLGFGILGFFPATIALI
                     AGVVNRKKYGLQESVKFMSKIYKKNFVRYNLYCWSATLLLAGNITTIWLCWQQSAPLF
                     YLLSTLLSVITLFLVFAMLNWVVIKSLGLWMMGDRLIDYLLFTATMLPTVVMQGLVLG
                     IYFRILMILPVIFLFGGTAVTVILISKLTERALAKLPCQIQSH"
     gene            8169..10082
                     /locus_tag="ONT85_002276"
     CDS             8169..10082
                     /locus_tag="ONT85_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HCR2379229.1"
                     /translation="MKLTDKQVANIAFIQQLEIPLTQKDEEQIDLIFTDTKANVRQIK
                     ITKNEQQRICLSYSHEDLLYRGLFICLPRFLAQGGEMPYIEETSIIETSYSLDASRNG
                     VMNKETLKKFISYLALMGYNTLYIYTEDTYEVEDLPYFGYLRGAYTKEEVNEIVEFAS
                     GFGIEVVPSIQTLAHLTQFLKWYAQQELMDDQNTLLIGEEKVYAAIEKMIIACKEMYQ
                     TERIHLGMDEAYQAGLGRYLSLYGYCDRTTLMIQHMDRVLSIVDKYKMKPMIWSDFIY
                     KLLDETGKDRYYNPDFKLKALAAKGLPAGLTYVHWDYGSDEVSSYEKVIDNHLKFCDI
                     DHYVLASGAHIWGRLAPNHGKTEAIVSAAVKASKNKGVRSVMLTTWGDEGQETEHWHS
                     LIGALKLADYVYSEEEWNDFEEIYDRLLGTGSYQLMSELRYFDEVDTITNNNLQMASV
                     SKLLLWQDPLVGIYDFHVAAHNKIATRTIGTYYHELAQKLDMNKNWNPKQPILTTIRQ
                     RYILLAEILAQKAELGIYLQHCCLVEDLAGLMELANRCEALANRVNELASIHEIIWYQ
                     TYKTYGWEVLEQRYAALAHRLKTVARRIKNHLQGQDALEELLEERLPFCQYQVPLEIS
                     GFNYRQSSVSGYN"
     gene            10210..13191
                     /locus_tag="ONT85_002277"
     CDS             10210..13191
                     /locus_tag="ONT85_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 42"
                     /protein_id="HCR2379230.1"
                     /translation="MLSDYYQNLSVISVNTEPLRNYYIPYASKEEAKQELEREKTSQF
                     IDLNGTWDFQYYPSVQQLPENFFQEAFTESYDRISVPGVWQTEGFDLPQYINVSYPIP
                     FDPPYVPNDTPCGVYRRQFQIEQTDARFYLNLEGADSNCFVWINRNFVGYDQVSHSTS
                     EFDVTKYLQVGVNEIQILVMKWCDGTYFEDQDKWRMSGLFRPVYLLQRPTMHLTSYQL
                     RTLTDPKKGSGTLMVSFQRFTPIEVSLPISYELFHPDGRLIAKGECYSEQQINLDVED
                     CLFWTAETPKLYTLLLTCNGEYFKEKVGFRALTIKNRKLLLNGTPIKLYGVNYHDVNQ
                     QGTPVMTREQLRADLLLMKKLNVNTVRTAHYPKSPDFYELADEIGLYVINEADLECHG
                     VVNLYDTEANYQLIANDPTFEKPIMDRVIRSILPYQNRSSIIMWSMGNESGYGVNFEK
                     AQALARKLDDSRLIHNERSIEPVIDQVNDFSNLDVISQMYPPLEDVHRHCQRSDTEKP
                     FFMCEYSHAMGNGPGDLKTYFDLIQQYDSFLGGCVWEWTDHAINRGTLETPSYLYGGD
                     FGEKYHDGNFCVDGLLTPDRQLTVGALEYRNIHSPIQLIAVDEELQEVTLSNITAFTA
                     GSAFNLSLEWYIDGKKTVYEVMNPFILEPGKSCSLPITVPTTIHGAHTVAVSLSVYKN
                     DELYGQQQLFLKKEKMRLPRTAHHNDAPQISVKGTELIIKGESFIYIYQLAKAAFTSV
                     KINQKELLERPTQFSIWRAPTDNDRNLVNKWKAAGYAEMSLEIRKKDWKFVDDHLLIE
                     TSYRGVIPGLQPIFTMHIQLKINGAGVINQKVSVEKNPIFPDFPRFGMDWPLNDTFSK
                     VSYCGYGPYESYPDKHYAAMFGIYEMEIEEFDQPYIKPQEYGNRWQCDWVSIKNHQGS
                     TMTVYGNSNFSALPYSQAQLTEKSHHYELIKENRTWFNLSYKQNGIGSHSCGPYLEDR
                     FRFMEDTFDWNIVITFKGENR"
     gene            13188..14849
                     /locus_tag="ONT85_002278"
     CDS             13188..14849
                     /locus_tag="ONT85_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hyaluronate lyase"
                     /protein_id="HCR2379231.1"
                     /translation="MKETKINQVKKKLSRYQEVVYRNHCLKWSKNKLNVRFNGLFYRF
                     EQLEEVWKPVTNFQSCPVQLINDPKETYDLCITHLYNQHLKNDGFYLKVTNDNQLTIE
                     SANLRGFQYAMEALLESFFSVGDQLLLPELILKHEPSVKIRGIIEGFYGIPWTHEMRL
                     SLFSYMKDNQLNTFMYAPKDDELLRKKWRELYDAQELDKFKELLSAAEASNIDFWYLI
                     SPGNDIDITCEEDIQVLLKKLEQLIELGVFQFGLLMDDIDYQLKGAAKRRFREPAFAH
                     AYLVNRVEEYLSTRLSNHELVICPTEYDNRHGSRYLATLSQEIPEQLPFFWTGPSTLA
                     ASISTEELQEMASVYQRPMLIWDNIPVNDYLEDKELLFMSPYENRTPNLSKERYQVTG
                     VVSNPMAQLEASKFTINSMANYLWNCERFDPLETWTSVVEKVVGSKLQPAYLTLTNAF
                     PNHYTSSLLSDEELLLAKDPEWVTKEMAALVQAVKWIQQEQHTSRLRTELEPWLQAIT
                     ESWTFWQEWQLKKDPKEAEEWLAKKKTHRIGRDLVTAHLEQIIKS"
     gene            14889..15821
                     /locus_tag="ONT85_002279"
     CDS             14889..15821
                     /locus_tag="ONT85_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005229489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HCR2379232.1"
                     /translation="MVKRLISANYSDVKKMTSKELKQSIKASEGRVIVCETVANTLPQ
                     SGEITNAEVASAFGADLILLNGFDCYRPAVFGLPNVTIEKVFDLQQTIENPIPKLKEL
                     VGRPVGINLEPIAPKTNMMSEKVEISQGRASSVETIQKAEELGVDFICFTGNPGTGVT
                     NLEITKSVKMAKKYFSGLIIAGKMHSAGSEEPVVNKEAIISYAEAGADILLVPSVGSI
                     PGFLEQDLIEAVRLAKKYDLLTMSAIGTSQESARPETLRQLAIMNKSCGVDLQHIGDA
                     GYGGLAPAENIFETSVAIRGMRHTINCVSRSINR"
BASE COUNT         5228 a         2609 c         3232 g         5061 t
ORIGIN      
        1 attttttgat gaagaatggc tgattgaaaa agaatataaa ggcgttcaag cgcagtttat
       61 taaaggaaaa ttggatgact ctccttccta ttgtgaacac tgtcgatcgg ttcgtattat
      121 ccgcaacggt tcctatacga cacgcactca gatattgaaa gtcaaagaaa agttaactat
      181 tttagaatta aaacgtaccc gttttctctg ccatgattgt ggaaggacct tttctgcgaa
      241 aacggactta gttgatgaac atcatcaact aacaaaggaa ctgaagcagg caattctcat
      301 ggaactgtac gaaaatcaat cacgtaaact aatcgctaag aagtacttcg tctcggatgg
      361 gacggtgaca cggatcttgc gtgaagcaac gaagcattac cagtcacgta tgaacttcct
      421 tccgacggtg ttgtgtatgg atgagttcaa atcaatgaag tctgtttctg gttccatgag
      481 ttttatttgt gttgatggga caacccatca gctatttacg atccttgaag atcgcagact
      541 ttacaaacta actcaatact tcctgcactt tcctagaaaa gcccgtttaa aggtcaagta
      601 cctagtgatg gacatgaatg ccagctattg tcagttgctt aaaacagtct ttccaaatgc
      661 ggagatcgta actgaccgct ttcatattgt ccaacacatc aatcgcagct tcaatcaatt
      721 acgtgtccag atcatgaatc gttttcgaac ctcacactct gaaaatcaga agaagtaccg
      781 tcggctcaag cgatattgga aactcttact caaagattcc acgacatggg agccactcaa
      841 acggcattat catcgactgt ttaaacgacc aattagtcaa acagaaatcg ttgatgagct
      901 gttaagctac aacgaagaat tgcgaactgc gtatcatttt tgtcaattat tacgctatta
      961 tttcgttaaa cgaggtacgg aaggctttca agaaaccctt aaaacaattt catctacgtt
     1021 acctcagtcg tttaaaaaga aattcacgat cttccattgc taccaatcgg gtatttcaaa
     1081 tgcctttaag gtgtcccact caaacggcgt cactgagggg ctgaataaca agatcaagct
     1141 gattaaacga attgcgtttg gttatcgcaa cttttataac tttcgggcac ggatctattt
     1201 acaacaaggt ctgatctttg aaaactaaaa aaatatgcgg cgcacagaac gcgccgcata
     1261 tcggactaag ttttattcaa atttgtcttc accaacacga tttgacgaag agcctattta
     1321 aataactaca tccatttatt ctatcttcta aaatagcctt tttcatttca gttaaatagt
     1381 tcagcgccat ttttgcactc gacttgcata gtagtttttt ccatattcct caacctcagc
     1441 gggggttgaa gacataagga aaatcctatg cgttatgctc aactggatct gattaaatat
     1501 tctaatgtat ttttctctac catcattaaa tggttaatag ccaaaaatca ccacttttca
     1561 atggctcctt gttgtataag tataactaga agatgaacac gtctgagaaa ttatagaagc
     1621 tttttacggt aattactggg gctgtgtcca gtagtttttt tgaagagaac agaaaaatag
     1681 ctcacgtgat ctattcctac tttttgacta atttcatcaa tagataagaa tgtgtctttc
     1741 agtagagcac ttgctctttt gattcgaaag tctagcaagt aatcatgaaa ggaataacct
     1801 gtttcttttt tgaaggcccg tcctaaataa gcgggatggt aatggaattg tgtagctaca
     1861 tctttgagcg taagcttttc actataattg ttttgaacgt aatcaatcac ttgttgaatt
     1921 gactcattaa aagaatcatc agttagagag ttttgattag ctgctgattt acgcattttt
     1981 tcttcttgta ctaacttaac taaataagga aaatcaatgg gtttcgttat gtaactttta
     2041 atagataaat caatcccctt ttttgcatac tgaaaatttt ggtgggcact aatgacaata
     2101 ctagtaaagt agcgattgga tgtttgttgc aaagattcga ttaattcgag tccagaaatt
     2161 ttccccatcg taatatctgt gataagtaaa tcaaagcgct ggttttgcag gtttttaaat
     2221 gcttcgtctc cgtctgtgta gcagtgatcg ataatgatgt cggggacttt cttcaaaaga
     2281 taatgcctta tgccttctac aattgtacaa tcatcgtcaa caattgcaat tctcaacatg
     2341 catattcccc cctttttgta ttttgatagt aatcgttgtt ccgacgtgta gttggctttc
     2401 tataacaaaa cgtgtatctc caggatagag atgctttata cgtcgaaaag aatttgagat
     2461 gccgatattc tcactataag gaatatcatc attaagtttt tgttgaatat gctccagctt
     2521 gtcttggtcc attcctttcc cattttcaac gatttttaac acataatggt tggttgtttc
     2581 agttgcatag acagtcaaac gattttcctt ttttgatgaa tcgaagccgt gtttaatata
     2641 attttcaaca aatggctgta aagaaagttt tggtaacatt aattgatcaa tatgatcaat
     2701 cagctgatag tcaaagttat gtcgataacg acgttgatgt agttgcaagg agaggtcgat
     2761 attaattaat tcctctttca aatcaattat gtcgggctga tatgtattat tgcgatacaa
     2821 cttgctcaat aaatatatac tgtcgctgat ttcttcgtca tcattcaata tggcagtcat
     2881 tcgaataaat tccaaattat taaatagaaa atgaggctcg atttgatatt gaagtgctgc
     2941 atactttgct tcttgcttca accaattatt ttcataaagt gttttttgat atgtagcatt
     3001 atctaaataa aactgattga tatgcatagc gatttctttc aaatcaccga ctttgttttc
     3061 gatattgatt gacttgtagt ctcctgtaga tgaatgattt aaggaagtaa tgatttcctg
     3121 atactgctgg atatagttac gaaaacttct atttaataat tgatgaatag caagaataaa
     3181 caaactcatt aataagaaaa tcatgattgc cagacctaat aaggtttgtg acgaataatt
     3241 tggattttca tatacttgta atgtgtggtt accgatagct atctgttgcg tttttttcca
     3301 tttagaaggc gagatgtctt cagataaatt gacggaatca ataccattag acaaagaata
     3361 attgtaatgt gttggaaaga gactcttttt tagcgcgttg atcgaaacgt ctagaatcaa
     3421 tgtagcatcg ggcatgtcct cactaatgaa acttaatgaa cgaataaaag aaattttttc
     3481 gttttttgta ggtgcgtagg cacttaagta tttttcgtaa tctaatgaga tagatatatt
     3541 tttaacttca tcatcttgca caagtttttt taagttatct actaaatagg taggggagcg
     3601 atcctttgac aaaatatctt ccatgtattc agcaggtgat gagaggtagt agtcattaag
     3661 gttttcttgg aaatcataat tttgatagat ctctgtcacg ataccggaga gaatacgatt
     3721 atatgtttcc atatagtggg tgatttgctg aatgttttgc aggttttgat ttttttcttt
     3781 ttcaatcaca ttgatatagg aaaagaacaa tgtgaaacag gcaagtaaag taataaaaaa
     3841 aatcaatatg tatgtaaaac gcataataac ttttgataaa tcgtttttca ttcaaagtcc
     3901 tcattttgat aatctctaat tttatgaatc attatagcac atattacaag tgatgtaaac
     3961 gctttgcaaa cagggctact tatgattgtg aatacagttt ccgtttaaaa actattctat
     4021 tgtagaggtt taataaacta aaaataatgt ataaaaaatt aatgtaagcg attaaccaag
     4081 tgggtataat caaggaaacg ctgagagagg agagacaaaa tgaagctaaa gaaagggtta
     4141 ttaggaaatc taaaaaaaaa ccgagcattg tttattatgc ttttaccagg tgcgttatgg
     4201 ttatttatat tttgctatat accaatgttt ggagttgtaa tcgcttttaa agatttcaga
     4261 atttcatcaa ggggattctt tacaagtttg ttcaacagtc cttgggttgg ctgggaaaat
     4321 ttcaaatatc tctttgcttc gcaagatgct tggatcatta ctcgcaatac ggttgtttat
     4381 aacgttgtag gtattttgct aagtactttt tgtacagtgt cattagcaat cattctgagc
     4441 caaatggcga acaaacggtt attgaaaaag atgcagacat ttatgatttt tcctaatttt
     4501 ttatcatggg tagtcatcag ttactttgtt tacgcatttt tgagtacgga taaaggatta
     4561 attaatattt tactaagcaa ttttggtgtg gaacccataa attggtatag tgaaccgaag
     4621 tactggcctt ttattattgt ttttacgagt ttatggaaag gcgtaggcta tggatccatc
     4681 atttattacg cgtcaattat ggggatcgat actagttatt atgaagctgc tttagttgat
     4741 ggtgccagta aatggcaaca gataaggaat gtagtcttgc cacatttaag atcgattatt
     4801 gtcattatgt tgattttgtc tgttggtagt atttttcgag ctgattttgg attgttttat
     4861 caattgccaa gaaattcggg ggctctttat gatgtgacga atgtattaga tacttatgtc
     4921 taccgtggat taatgaatag cggagatatc ggaatgtcta cggcagcggg attatatcag
     4981 tcatcagttg gctttgtctt gattttgatt actaataaga tagtcaacaa gttagatcct
     5041 agttcagcac tattttagga ggaatcaaaa tgatatcaaa attaaaacgg cagatgtact
     5101 tttcgctgcc agtcgaacgg tttttcacaa tattactaat cttatttact cttagctgta
     5161 tttttccctt tattttcgta atcgtcattt cattaacaga tgaaaaagca ttacaactca
     5221 acggttacga attattgccg gctcaatgga gcactgatgc atatcaatac ttaatacaag
     5281 atggcggaca gttacttcgg tcattaggcg ttacaattat gattacagtg attggaacgt
     5341 tgattactgt ttttatgacg ggaacctatg cttatgtttt atcaagagcc tctttccctt
     5401 accggaagtt ctttactttt tatctttttt ttaccatgtt atttgcggga gggatggttc
     5461 cttcttactt agtgatgacg aagatgcttg gcttaaaaaa tacaatttgg gctttgattc
     5521 tacctcttgc ttttagccca tacaatgtca ttattttaag gacattcttt aagaagtcga
     5581 ttccagaatc gattatcgaa tccgctaaaa tggatggctg tagtgagttt cgagtttttt
     5641 tccaaatcgt tttgccttta gcgatcccag gggttgcaac aataggttta ttttcaagct
     5701 taggttattg gaatgactgg ttcaacgcat tgctgtatat tgacaccaac aaattgattc
     5761 cattgcaata tttgttaatg aagattcaaa attcaatgga attcttagca aataataatg
     5821 acattacatt agcgcagcaa caagcaattc aaaatagcct tcctcaagaa tcaacacgaa
     5881 tggcaatggt cgtagtagct actttaccga ttgcaattgt ttatccgttc ttccaaaaat
     5941 actttgttca aggattgacg atcggtggag tgaaagaata aaaaagaata ggagatgtta
     6001 aaagatgaaa aagaacatgc agaaatattt gattggtggt gcttctgtac taacccttgg
     6061 cctattaggt gcttgtggta gcagtaatgg cgaagcaaat aatgaaacag gaaccacaaa
     6121 agttacttgg tatcaggttg gagaagaacc caaagatttt gatgaggtca tgacaaaagt
     6181 caacgaaaaa ttaaaggaag aaatcaatgt agagttggat atgcggctga ttccggacgg
     6241 ggattatcag caaaagcttg gtgtgatgat caattcagga gaagaatatg atatttgttt
     6301 cgtcaatggc acagattatg tgaattatgg gaacaaaggt gctttcatta gtctcaacga
     6361 tcatttggat aaagagttga aagattatgc agctgaattg aatgaaggat ttattgaagg
     6421 tggggcaatt aatggcgaaa cttatggatt gccagtcaat aacgatattg ccacaacgga
     6481 atattggacc tttaataaag atttggtaga tcaataccaa ttggatgtag agaatgttga
     6541 aggtgtagaa acattggctc cagtattaga aaagtttgca gaaaaaaata ctgatacgaa
     6601 tattacgcca atcggtatga cccgtaccag tgtctgtatt ccttatgaca atattatcgg
     6661 tggtggtgta ccgctagcgg ttgcatttga aggcgataca caaacagttg ttaatactta
     6721 tgagactcca gaaatgatga cttatttgaa ggctatgcat gagttttata aaaaaggata
     6781 tattttgaaa gatgcagcca ctgttacgac agatccattt cgaattgata gtaaaaactg
     6841 gtttttaggt cgtaactcaa gtggttatca accattagca caaaaaaaat tagaagctct
     6901 agcgggacat ggattaattt taaaaccagc tggaaaagct tttaaaacga ctggatttgc
     6961 aagagtggca atgcatgcgg tatctgcttc aagtaaacat ccggaagcag ccttgaaatt
     7021 gttgaattat gtgaatacca atcaagaagt agcggattta atggtttatg gaattgaagg
     7081 aaaacactat gatcgcaatg aagatggttc gattacttta acaaactctg atgattatta
     7141 tcaaggcgct tgggcattgg taaatactta tctagtttca aaaacttcac cgattacaga
     7201 agaaagtaaa aaactagaag aagaaagtaa aaagtttcaa aatgaagcac cgatgtctcc
     7261 tattttaggt ttctcttttg acgcttctga ttttcgaaca gaaattgcaa gtgtacaaaa
     7321 tatcgtgtca caatatgaag tacttttaag ctcgggaaca gttgaccctg aacaatatgt
     7381 accagagttg ttagaaaaac tagaaaaagc tggattggat aaaattcagg catctgctca
     7441 agaacaatac gatgaatggc gaaaatctaa caaataattt tttactaaat gaaaagaata
     7501 gactaagcag ttagtggggg gattcaaatg aacgaaaaca aatggaccaa aggatttaat
     7561 tgggtttttc aattgttgtt actaaacttt acttatttta tactaatttg tttaggattt
     7621 ggaattttag gttttttccc tgctacgata gctttgattg ctggcgttgt gaatcgaaaa
     7681 aaatatggct tgcaagagtc tgtgaagttc atgagtaaaa tctataagaa aaattttgtt
     7741 agatataatc tatattgctg gtcagcaact ttgttgttgg ccggcaatat tactacgatc
     7801 tggctttgtt ggcagcaaag cgcaccattg ttttatttat taagtacatt attatcggta
     7861 attacactgt tcctagtctt tgcaatgttg aattgggtcg tgatcaaaag tcttgggttg
     7921 tggatgatgg gagaccgact gattgactat ttgcttttca cagcaacgat gttgccaacg
     7981 gtagtcatgc aaggcttggt gcttggaatt tatttccgaa tattgatgat tttgcctgtg
     8041 atttttctgt ttggcggaac agctgtaacg gtaatattga ttagtaaatt aactgaaaga
     8101 gcgttagcaa agttgccttg tcagatacaa tcgcactaat tttttcacaa aaaggagtat
     8161 ttctttctat gaagttaact gataaacaag tagctaatat agcctttatc cagcaacttg
     8221 agattccatt gacacaaaaa gatgaagaac aaatagactt gatttttact gacacaaagg
     8281 ctaatgtgcg acaaatcaag attactaaaa atgagcagca acgaatttgc ttgtcttatt
     8341 cacatgaaga cttgctgtat cgaggattgt ttatctgcct tccacgtttt ttagctcaag
     8401 gaggagagat gccatacatt gaagaaacct cgatcattga gaccagttat tcattagatg
     8461 cttctcgtaa tggcgtgatg aataaagaga ctctaaaaaa attcatttct tacttagcgt
     8521 tgatggggta caacacattg tatatttaca cagaagatac ctatgaagta gaagaccttc
     8581 catattttgg ttaccttcgc ggagcgtata caaaagaaga agtgaatgaa attgttgaat
     8641 ttgcttctgg ttttggcata gaagtagttc catcgatcca gacccttgct cacttgaccc
     8701 aatttttgaa gtggtatgct caacaagaac tgatggatga tcaaaatact ctactgatcg
     8761 gtgaagaaaa ggtttatgcg gcaatcgaaa agatgattat tgcctgcaaa gaaatgtatc
     8821 agacagagcg tattcatttg ggaatggatg aagcctatca agcaggtctg ggacgatatt
     8881 taagcctgta tgggtattgt gatcggacaa ctttaatgat tcaacatatg gaccgtgtgt
     8941 tatcaattgt tgataaatat aagatgaaac caatgatttg gagtgatttc atttataagc
     9001 tattagatga aaccggtaaa gataggtatt acaatcctga ttttaaactc aaagcattag
     9061 cagctaaagg attaccagca ggattaacct atgttcattg ggactatggt tcagatgaag
     9121 tttcctccta tgagaaagta attgataacc atttgaaatt ttgtgacatt gatcattacg
     9181 ttttagctag tggtgcccat atctggggga gactagcccc gaatcatggg aaaacagaag
     9241 caattgtttc tgcagctgta aaagcttcta aaaataaagg tgtacgttct gtgatgttaa
     9301 ctacttgggg agatgaaggg caggaaacag agcattggca ttccttaatt ggggcattaa
     9361 aattagcaga ttatgtctat tctgaagaag agtggaatga ttttgaggaa atctacgatc
     9421 gtttattagg aacaggtagt tatcaattaa tgtccgaatt acgatatttc gatgaagtgg
     9481 ataccattac aaataacaac ttacaaatgg cttccgtgag taaactgcta ctttggcaag
     9541 atccgttagt tggtatatat gactttcatg tagccgctca taacaaaatt gccactagaa
     9601 caattggtac ttattatcat gagttagctc agaaattaga tatgaataaa aattggaatc
     9661 caaaacaacc aattttaaca actattcgtc aacgatatat acttttagct gaaattttag
     9721 cacaaaaggc agaactaggc atctatttac aacactgctg tctggtagaa gaccttgcag
     9781 gattaatgga attagcgaat cggtgtgaag cactagccaa tcgagtaaat gaattggcat
     9841 ctattcatga aataatttgg tatcaaacat acaaaaccta tggttgggaa gtattagaac
     9901 aacgatatgc tgcattagct catcgtctga aaaccgttgc gcgtcgtatt aaaaatcatc
     9961 ttcaggggca agatgcccta gaggagcttt tggaagaacg attaccgttt tgtcaatatc
    10021 aagtgccgct tgaaatttct ggctttaatt atcggcaaag ctcggtgtca ggctacaatt
    10081 aacgaaggaa tcttcccgtt gaacggacca cttgctcatc taatgggaaa gtggttcgtt
    10141 ttctattcat aagtagctta ccactaactc ttttattaag aagagaaaaa aagtaggaag
    10201 tgagaaaata tgttatcgga ctattatcaa aatctgtcag ttataagtgt caatacagaa
    10261 ccattacgta attattatat tccttatgca agtaaagaag aagcaaaaca agagttagaa
    10321 agggaaaaaa cgagccagtt tatcgatctc aatggtacat gggattttca gtattatcct
    10381 tctgtccaac agttaccgga aaattttttt caagaggcct ttactgaaag ctatgataga
    10441 atttctgttc ctggagtttg gcaaacagag ggatttgact tacctcagta tatcaatgta
    10501 tcttatccaa ttccttttga tccgccttat gtacctaatg ataccccttg tggtgtatat
    10561 cggcgtcagt ttcaaattga gcaaacagat gcacgctttt acttaaatct agaaggagca
    10621 gatagtaatt gttttgtttg gataaataga aactttgtag gctatgacca agtgagtcat
    10681 agtacatctg aatttgatgt gaccaagtat ttacaggttg gggttaatga aattcaaatt
    10741 ctagtgatga agtggtgtga tggtacctac tttgaagatc aggataagtg gcgaatgagc
    10801 ggtctatttc gtccagtgta tcttcttcaa cgaccaacta tgcatctgac aagttatcaa
    10861 ctacggacgt tgactgatcc taaaaaagga agcggaacat taatggtttc ttttcaacga
    10921 tttactccta ttgaagtatc gttacctatt tcttacgaat tattccatcc tgatggacgt
    10981 ctcatagcga aaggggagtg ttactcggaa caacaaataa atttggatgt tgaagattgc
    11041 cttttttgga cagcagaaac gcctaaatta tatacattac ttttaacatg taatggtgaa
    11101 tattttaaag aaaaagttgg ctttcgagca ctgacgatca agaatcgtaa gttattgctg
    11161 aacggaacac ctattaaatt atatggagtc aactatcatg acgtgaatca gcaaggcacg
    11221 ccagtgatga cccgagagca gttacgtgca gatttgttat taatgaaaaa attaaatgtg
    11281 aatactgtac gaacggcaca ttaccctaaa tcaccagatt tttatgaatt ggcggatgag
    11341 attggtctgt atgtaatcaa tgaagcagat ttggagtgtc atggtgtggt taatctatat
    11401 gatactgaag cgaattatca actaattgct aatgatccga cattcgaaaa gccgattatg
    11461 gaccgagtga tccgttctat tcttccttat caaaatcgaa gctctatcat tatgtggtct
    11521 atggggaatg aatctggtta tggagtaaat tttgaaaaag ctcaagcttt agcacggaaa
    11581 ttggatgatt cacggttgat tcataatgaa cgctcgattg aaccagttat tgaccaagtg
    11641 aatgatttta gtaacttgga tgttattagt caaatgtatc ccccactgga agatgttcat
    11701 cgtcattgtc aaagaagtga tactgaaaaa ccttttttta tgtgtgaata tagccacgct
    11761 atgggcaacg gtcctggaga tcttaaaacg tacttcgatt tgattcaaca gtatgattcc
    11821 tttcttggcg gttgtgtctg ggagtggact gatcatgcaa tcaatagagg aactttagaa
    11881 acgccatctt atttatacgg tggtgatttt ggtgaaaaat atcacgacgg caatttttgc
    11941 gtagatggtt tattgacccc agatcggcaa ttaacagtag gtgcattgga gtaccgtaat
    12001 attcattcgc ctattcaact gattgctgta gatgaggagc tgcaagaagt aaccttgagc
    12061 aatataactg cttttacagc cggttcagca tttaaccttt ctcttgaatg gtatatagat
    12121 ggaaaaaaaa cagtttatga agtaatgaat cctttcatac ttgagcctgg caaaagttgt
    12181 tcattgccca tcacagtacc tacaacgatt catggtgctc atacggttgc tgtttcgcta
    12241 tctgtttata aaaatgatga gctttatgga cagcaacagc tctttcttaa aaaagaaaaa
    12301 atgagattgc caagaactgc tcatcataat gatgcaccac aaatttcagt aaaaggcaca
    12361 gagctgataa tcaaaggaga atcctttatt tatatatatc aattagcaaa agctgcgttc
    12421 acttctgtaa agatcaatca aaaggaactt ttggaacgtc ctactcaatt ttcaatttgg
    12481 cgtgcaccaa cggataatga tcgtaactta gtaaacaaat ggaaagcagc tggatatgct
    12541 gaaatgtctt tagaaatcag aaaaaaagac tggaaattcg tagatgatca tctcctgatc
    12601 gaaacgtctt atcgaggagt tattccagga ttacagccaa tttttacaat gcacatacag
    12661 ttgaaaataa atggtgcagg cgtaatcaac caaaaagtat cagtggagaa aaatccgatt
    12721 tttccagatt tccctcgctt tgggatggac tggccgttga atgatacttt ttcgaaggtt
    12781 tcttattgtg gctatgggcc gtatgaaagt tatcctgata agcactatgc tgcgatgttt
    12841 ggtatttatg agatggagat tgaggaattt gatcagccat atatcaaacc acaagaatat
    12901 ggtaaccgct ggcaatgtga ttgggtatca atcaaaaatc atcaaggatc taccatgacg
    12961 gtttatggaa actctaattt ttctgctctg ccttatagtc aagctcaatt aaccgaaaag
    13021 tcacaccatt atgaattaat aaaagaaaat agaacttggt tcaatttaag ttataaacaa
    13081 aatggtatcg gctcacacag ttgcggtcct tatttagaag atcgtttccg ttttatggaa
    13141 gatacttttg actggaatat agtgattact ttcaaaggag aaaatagatg aaagaaacaa
    13201 agatcaatca agtcaaaaaa aaattatctc gttatcaaga agttgtttat cgaaaccatt
    13261 gcttaaaatg gtcaaaaaat aagctgaatg ttcgttttaa tggattattt tatcgtttcg
    13321 aacagttaga agaagtctgg aaaccggtaa cgaatttcca atcatgtcct gtacagctca
    13381 taaatgatcc aaaagaaaca tacgatttat gtataacaca cctttataac cagcatttga
    13441 aaaatgacgg attttattta aaggttacta atgacaatca acttacgatt gaatcagcaa
    13501 accttcgagg gtttcaatat gctatggaag ccttattaga gagttttttt tcggtaggag
    13561 atcaattatt attaccggaa ttgattctga aacatgaacc atcagtgaaa attcgaggaa
    13621 tcattgaagg gttttatggt atcccctgga cacatgaaat gcgcctctct cttttttcat
    13681 atatgaaaga taaccaattg aatactttta tgtatgcccc taaagatgat gaattattac
    13741 gtaaaaaatg gcgtgaactc tacgatgcgc aagaattaga caaatttaag gagcttttat
    13801 ctgctgctga ggctagcaat atcgattttt ggtacttaat tagtcctgga aatgatattg
    13861 atattacgtg tgaagaagac attcaagttc ttctgaaaaa gttagaacaa ctgattgagc
    13921 ttggcgtatt tcaatttggt ctattaatgg atgatattga ctatcaatta aaaggtgctg
    13981 ctaaacgccg ttttcgagag ccagcattcg ctcatgccta tttagtgaat cgagtagagg
    14041 aatatcttag cactcggttg tccaatcatg aattagtcat ttgtcctaca gaatatgata
    14101 atcgtcatgg ttctcgctat ttagcaacgt taagtcaaga aataccagaa caattaccgt
    14161 tcttttggac tggcccaagc acgttagcgg cgtcaatttc tacagaagag ctgcaggaaa
    14221 tggcttctgt ctatcaacgg ccgatgctga tttgggataa tattccggta aatgattatt
    14281 tagaggataa agaactattg ttcatgagtc catatgagaa tcgaacaccg aatctttcta
    14341 aggagagata ccaggttaca ggagtagttt cgaatccaat ggctcaatta gaagcttcaa
    14401 agtttacgat caatagtatg gctaattact tatggaactg tgagcgtttt gatcctttgg
    14461 aaacttggac gtcagtcgtt gaaaaagtag ttggaagtaa actacaacca gcttatctta
    14521 ccttaaccaa tgcttttcca aatcattaca caagcagtct gctttcagat gaagaattac
    14581 tgctagccaa agacccagaa tgggtcacaa aagaaatggc tgctttagtc caagcagtaa
    14641 aatggatcca gcaagaacaa catacatcta gattgagaac agaattggag ccttggttac
    14701 aagcaatcac agaaagttgg actttctggc aagaatggca attaaaaaaa gatccaaaag
    14761 aagcagaaga atggttggct aagaaaaaaa cacaccgcat aggacgtgat ttagtaactg
    14821 cccatttaga gcaaattatc aaaagttaga tatcttaaaa aataaaaagc aagaaaaagg
    14881 agaacaaaat ggtcaagaga ttaattagtg caaactacag tgatgtcaag aaaatgacgt
    14941 caaaagagtt gaagcaatcc atcaaagcga gtgaaggacg ggtgattgtt tgtgaaacgg
    15001 ttgctaatac tcttccacaa tctggagaaa ttacgaatgc agaagttgcg tcagcatttg
    15061 gtgcagattt gattctttta aatggtttcg attgttatcg tcctgcagtt tttggactac
    15121 caaatgtgac gattgagaag gtttttgatc tacagcaaac gattgaaaac ccgattccta
    15181 aattaaaaga actagtcggt cgtcctgtgg gaattaattt agaacccatt gcccctaaaa
    15241 caaatatgat gagtgagaaa gtagaaatca gccaaggaag agcaagctct gttgaaacga
    15301 tacaaaaagc agaagaacta ggagtcgatt ttatttgctt taccggtaat ccaggcactg
    15361 gagtaaccaa tttagaaatt actaaatccg taaaaatggc aaaaaagtat ttttcgggat
    15421 tgattattgc tgggaaaatg catagcgccg gctcagaaga gccagttgtg aataaagagg
    15481 cgattatttc ttatgcggaa gcaggcgcag atattttact agttccttca gtagggtcta
    15541 tcccaggctt tttagagcaa gacctaattg aagcggttcg gttggcaaaa aaatatgatt
    15601 tgttaacgat gtctgcaatc ggtactagtc aggaaagtgc ccgaccagaa acattgcggc
    15661 aattagccat tatgaataaa agttgtggtg ttgatttaca acatattgga gatgctggat
    15721 acgggggatt ggcaccagca gaaaatattt tcgaaacctc ggttgcaatt agaggaatgc
    15781 gacataccat caactgcgtt agccgctcga tcaatcgcta acaaaattaa ataaaaaatg
    15841 gttaaattga ttatttaagt gggaacatac ttgagatatc agctaacttc tgtcacttta
    15901 aaaaagggac tgtgacagaa gtgaaaatag acaaaaaata tccgaacaag agactaggat
    15961 agcagaaaaa ctatcgtttt gcaacgagga ccaactggac cgtaggagca attctcttat
    16021 tgttcggata tttcgcttgt tcaaggactt atgtctgtag tgcccttttt tggtaggaaa
    16081 aatggtaggg aaccatatca tttttaatga aaatcaagca tttcaaaagt
//
LOCUS       DAJSBL010000060        16106 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.062, whole genome shotgun sequence.
ACCESSION   DAJSBL010000060 DAJSBL010000000
VERSION     DAJSBL010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16106
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.062"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            226..894
                     /locus_tag="ONT85_002280"
     CDS             226..894
                     /locus_tag="ONT85_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HCR2379233.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFSLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
     gene            complement(1094..1480)
                     /locus_tag="ONT85_002281"
     CDS             complement(1094..1480)
                     /locus_tag="ONT85_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2379234.1"
                     /translation="MRRTAKKFIDYNEYRDRPFGLKWGTAFAMEELVNGIKKNEEEAR
                     KQPLPKQQMTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGEAYEDYF
                     MIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(1452..2834)
                     /locus_tag="ONT85_002282"
     CDS             complement(1452..2834)
                     /locus_tag="ONT85_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HCR2379235.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            complement(2990..4177)
                     /locus_tag="ONT85_002283"
     CDS             complement(2990..4177)
                     /locus_tag="ONT85_002283"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HCR2379236.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            complement(4184..5215)
                     /locus_tag="ONT85_002284"
     CDS             complement(4184..5215)
                     /locus_tag="ONT85_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HCR2379237.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVGIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            complement(5231..6685)
                     /locus_tag="ONT85_002285"
     CDS             complement(5231..6685)
                     /locus_tag="ONT85_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR2379238.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANVATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEELENKLAPADTMEDELHDAVVPSH"
     gene            6860..7765
                     /locus_tag="ONT85_002286"
     CDS             6860..7765
                     /locus_tag="ONT85_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCR2379239.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            7856..8533
                     /locus_tag="ONT85_002287"
     CDS             7856..8533
                     /locus_tag="ONT85_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HCR2379240.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            8530..8700
                     /locus_tag="ONT85_002288"
     CDS             8530..8700
                     /locus_tag="ONT85_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2379241.1"
                     /translation="MTNDWLIQQLDELSEKSEYQDRALLYEIKKLMAEIDERTMQLQG
                     EIDGQSWNHLNW"
     gene            8814..9710
                     /locus_tag="ONT85_002289"
     CDS             8814..9710
                     /locus_tag="ONT85_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HCR2379242.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(9765..11135)
                     /locus_tag="ONT85_002290"
     CDS             complement(9765..11135)
                     /locus_tag="ONT85_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR2379243.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            11373..12119
                     /locus_tag="ONT85_002291"
     CDS             11373..12119
                     /locus_tag="ONT85_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HCR2379244.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQPVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            12116..12955
                     /locus_tag="ONT85_002292"
     CDS             12116..12955
                     /locus_tag="ONT85_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HCR2379245.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            12956..13906
                     /locus_tag="ONT85_002293"
     CDS             12956..13906
                     /locus_tag="ONT85_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HCR2379246.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            14047..14799
                     /locus_tag="ONT85_002294"
     CDS             14047..14799
                     /locus_tag="ONT85_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2379247.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            14818..15969
                     /gene="queG"
                     /locus_tag="ONT85_002295"
     CDS             14818..15969
                     /gene="queG"
                     /locus_tag="ONT85_002295"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HCR2379248.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEVYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
BASE COUNT         5221 a         3010 c         3235 g         4640 t
ORIGIN      
        1 cgtccaattt cgtaaaacaa ccgtaacggt tgtgtaaaaa tatcatttta gagcataaaa
       61 aaaaggagtg gatttctcca ctccaaaaaa gtctaacttt tgggggtcac tacagtgtca
      121 cagtcctttt tttagtaaaa ggatacattt cttccttgta tattccatgc gtgttcgtga
      181 gaaagcatat ataatttaat taacagatag ttagggggag aaaaaatgat tttttcaatt
      241 acaatgaaac tggtgttcgg gttgatcggt ttattattag tggtacgttt gcttgggaag
      301 aaatccatgt cagaaatcac accgtttgat ttagtctata cgcttgtttt aggtgggatc
      361 ttagaagaat caacctatga tgacaatgtc catgtagggc atgtcctatt tgccattgcg
      421 ctttgggcag taatgattta tggtatcgaa cgtatcgtcc aaaaaaatga aaaggtcaat
      481 cgttgggtca aaggcgagcc atctgttcta atcaaagacg gcgtcatcaa catgacagag
      541 ttgacaaata accatatcga gatggagcag ttgcgagcaa tcttaagaca gcaagaatgc
      601 ttttctcttg aaaatgccaa acatgttatc ctagagaatg ctggtcaaat gagtgtattg
      661 aaaaaatcag atgaagacaa agctttatcg atcctgcttg tagatgaagg gcaaattcag
      721 cataaagtgc ttcagtccaa tcaattgact gaagcatggc ttatggagaa cttgaagaaa
      781 gaaggctatg cagatgtaaa acagctgatt tatgttgaat ggtcagaaga aaaagggctc
      841 tacatactaa ataaagaaga cgtcatgcat gaatcttacc gaatagatgg atagatagac
      901 agaggttgtg aaatcagcgc cttggcttgt tactcaaaac taagcgactt tttcacaacc
      961 tctgatatct aatatacgtt gttccagaag ttgcttcttc gaaaaataag ccaaaattcc
     1021 acaaaaattt aagaagcaat tttcgaaaaa tcctttctta ttttttgaag ttgatcactt
     1081 ctgtcacaac ctctcatgtc acaaaccatt tttttgtttc ctttaactca atataccgta
     1141 cgtcttccca caatatcggc tgatggtcaa tcataaagta atcttcatag gcttcaccaa
     1201 tgaagtgccc ttcgacctca tccaagagtc ttccaaattc atcttttaaa ttgagctgaa
     1261 tgattactgc tttacgagat aaaaaggctt ctgtcagtat actatcgatt tcttgcaatg
     1321 tcatttgttg tttaggaaga ggctgtttgc gtgcttcttc ttcatttttt ttgataccgt
     1381 tgactagttc ttccatcgca aaagcagtcc cccattttaa tccaaatggc cgatctcgat
     1441 actcgttata gtcaatgaat ttttttgctg tccgtctcat cttttccacc ttctaatcca
     1501 ctcataccac ctgcatgccc gccaacgaga gaactacgtg cgatggcgcg tcctccttgc
     1561 aagagactat gcgcatggat gattgattga aagccgtatt tttttctaat cgtatcgaca
     1621 acaaaatcaa ctttcgcttc atttatctgc ttttcaggat cttcaaaaag gttaagctgt
     1681 aaacctgtcg cgtatacgag gtcggaacag ttcacgccta cacgccgaat atcctggtag
     1741 tggtaatgct ggtggaaaat ggtcaataaa gcatctgcta gatctctgct agcatttgtc
     1801 gcttcgattt tcatttgctg gtgaaacccg gtcttgccat ttggatcaag atagcctaaa
     1861 gagtatccta cccataagct gactacttgt gtttttgctg cagatcgtct caagcgagtg
     1921 ccgacttggt cggccatttc tttgataacg atctcgattt cttctcgtct tgtataatct
     1981 ctatctagga tttgactatt tccgatactt tttgattttg gtgtatattc ttgtcccaaa
     2041 aaacttcgat cgatgcccca agcatgggca taaagctgag taccgatgac ccctagcttg
     2101 cttttcaagt gataataatc cgcgtgagct aaatcataaa cagaatgtat acccatttgt
     2161 ttcagacgtt ttgctgttcg tttgccgatt ccccaaaagt cagtgatatc tgagattttc
     2221 cataactttt ctggtacatc ttcataacgc cattcagcaa acatttgctt tccgtctttt
     2281 gagccattgt ctaaggctag tttagcaagt agcgggttat cgccaattcc tatagtcgtg
     2341 tagattccgg tctgctcaaa cacatcttta tggatcagtt tagccatgtc gtacgcattt
     2401 gttttaccaa agagtgccat gctatttgtt acatcaagga agctttcatc gacggaatag
     2461 acatgatggt tcgcttcgtc tgcatatttt ttatagatag aattgatttc tctgttcttt
     2521 tccatataat aggccatcct tggggcaaca atatataaat cacttggata ggggaaggga
     2581 agatctctgg cacggctgat attggtgatg ccgtaagctt tttttgctgc aggactggaa
     2641 gccaagatta atccgctacc ccgtgtttct gtcgaatcgg acggatagga catgactact
     2701 aatttagttg taaggggatc aagatccttt tcaacgcact ctacggaggc gtaaaaagat
     2761 ttgcagtcaa tacataaaat atctcttgaa ggttcttttt cataaacaaa taccggattt
     2821 tgattcgccc acataacaat cacctcttca acacaattag aacacacgtt ctctttccgt
     2881 gtcaataaaa aacagcaatt ttcaaatacg agcaaaaggc tcacatttgg aaattgctga
     2941 gaggttattt ttatctattt gtttttagct gagatcgttg atttttttat caaacatgaa
     3001 cagcttctgg atcgatcttt gttgtcccat ttccaatgtg tgcaacataa aattccggag
     3061 catagccgac aactttcatg tattcatcat agacattgtt tttgaaggca ggtatatttt
     3121 cttctttgac aagagcaatc gcacagccac caaatccagc tcctgtcatt cgtgcaccta
     3181 agacaccttc ttgtttttga gcagcatcta ctagtgtatc taattcgatc ccagttactt
     3241 cataatcata gcgaagagag tggtgagaat catttaacag ttgaccaaat gcttctaagt
     3301 tcccagcttc taattcagct tttgccttca aagtccgctg attttcagaa acggcatggc
     3361 gagctctgcg tatcaatgta tcatcgccga tttggtcaat agttgattca aattcttctt
     3421 catctaagtc tcctaaagaa gagataggca aagttttttg caaacgtgcc aaggcttctt
     3481 cgcattcgct acggcgttca ttgtattttg aatctgccaa ttcccgccgc ttattcgtat
     3541 tcatgatgac gattgcataa ccatctagtt tcactggaac catttcgtat ttcagcgtat
     3601 ttgtatctaa taagattgca tgatctagct caccgaagcc gatggcaaac tgatccatga
     3661 tacctgaatt gacaccgatg aattcatttt ctactttttt acctgtttgg actagttcca
     3721 agcgatctgt ttttaaatca aacagatctt ctaatacaac acctactaat aattctaatg
     3781 aagcgctact ggaaagtcct gcaccattag ggattgttcc ttccactaac aactcaaacc
     3841 catgatcgat tgagtgtcca gcttctttta atttaaggac cattcctttt acgtaatttg
     3901 cccagtttgc ttttttatca tattctagct catctaaagt aaaactaatg acaccgatat
     3961 cgtcaaagtt tgtggaataa actaatattt tattatcgtc tcgaggtgct gctacacctt
     4021 gcgttccata agtgatggaa gcggggaaga catggcctcc attgtaatct gtgtgttcac
     4081 caatcaaatt gatgcgtcct ggtgagaagt attgtgtagt ttttttaggt ccgaatgttt
     4141 cggcaaattt tgtgtttaag cttcttttga tttccatgat gagttattcc tttcttgttt
     4201 tgattttgaa ttctgtcact gtttcaaata ctgatccagc ttttaaatga atcgaaccaa
     4261 atgctgagaa gcgctctgca cctggagctg tttgagtctc aaatgtgatg ccaccgtgat
     4321 gtgccagttt tcggttgcgc atttctggtg tttctgtgcc gaaatttgca gtgaaaataa
     4381 ccacactaga agcatctgta gcaataccta cttggattct tttatcgggg ctagtcaagc
     4441 gagcggcttc tttacccaag ccggtttctt ttaagaaaaa tggatgatcg attccgtcaa
     4501 caagattttt ttgatccaaa tcactagcaa aaacggacgc taatttttta ggtatttgaa
     4561 aatcgaaagc acttcctgca gcattttctt ttacccctat aggaatacta tctgttcgca
     4621 aaggagcata agcttcgcta ttgagccaaa gctcatgttg atcgattgac tgagaagcat
     4681 ctcccgtcaa gttgaagtaa acatgatttg tcggattgaa taaagtatct tggtcactgg
     4741 tgccgcgaga ggtcactcgc cagatattat cttttgtcaa agtatagcgg atttccacat
     4801 ccattgtacc tggaaaacca tgttctccgt cgggacttgt agtagtaaaa atgacagaag
     4861 cttcattttc gccattcaaa atgacataag accatttttt taattcaaat ccaggagcac
     4921 caccatgcag attatgtcca gtttttggat ctgtggctaa ttggtaagtt tttccattga
     4981 gagaaaagcg gccattttcg attctccctg cggtgcgacc gatagaagca ccgatatacg
     5041 gatctttttc gatatattct tcagcggtgt caaatcccaa aactaattct cgatcatttg
     5101 attttaaact gacgattcta gcacctaaat cagagataga aagtgctagc ttgtttttgt
     5161 tctccaaggt tatcaagtga taaccttggc caaaatcttt ttctgtaatc ttcattttca
     5221 agatcctttc ttaatgggaa ggaactactg catcgtgaag ttcatcttcc attgtatcag
     5281 ctggagccaa tttgttttcc aattcctcaa cgatttgtgc atgttttttc tctgtcaatg
     5341 taatcttcca agcaaaaata atagcggaga aaatcatcaa agcagcagga gcatagaatg
     5401 cataagcttt gaaagtcgtt acgccatgag cagtgatatc agcagcagta gcattaccag
     5461 tcatcccaga agcgatagcg acaaacccta cgatcccgtt agacaaagca ccagcgattt
     5521 tatctagtaa agggcgaatt gatagagtaa cagcttcgtt acggatacca tttttccatt
     5581 gaccatattc aacagagtct gtgatcgtca tcaaagcgga taagaaaatc aattgttgag
     5641 ggaagtagaa caatactagc ccgatgataa cgattggcaa acttgaacct gcgatcgtaa
     5701 agaaaaagta agaagaaatc atcattgcga tccctccaag gtaaacgtag cgacgagaga
     5761 tgacgcgtgt aagcattggg aatagtggaa cagctactaa accaagtaag gcagcaacta
     5821 gaccaacgat ccaaaattca gttggttggc caagtacata tttgaagaag tagaataata
     5881 cggcagttgt tgctacattg gcaatcgcat aagcgatata agcaagggca agccacatca
     5941 actgatcatt ttgagcaact gctttgaaca catctttgat cccagctttt tcattttgct
     6001 gacggatcgc agtatcgact tcttttgtgc ccaaggcagt gataatcgca gtaccgcctg
     6061 aaataacagc agcaatcaca ccgaaccaga accagcctga agcgccttca atatgtttat
     6121 gagtaacaag ataggtaaag aaagtaacga tagggacaac gactagagta gtaccatttg
     6181 cacctagaga agagccgaaa cgagcaaacg tagcaaattt ttcacgttcc tttgtttccg
     6241 tactcaaagc tggaatcatt gaccaaaaag cgatatcctt aaatgaataa aaactatcca
     6301 ataagatgaa aacgattgca aataatatga taaataaagt cgtattatga gtagctaagc
     6361 caaagaaatt ggtaaagatt gctaccaaga gtacggaact aacaacgccg ccgataacta
     6421 accatggttt gaatttaccg tattttgtac gagtattatc gatgatgcca ccaatcaaag
     6481 gatcaaaaat aatttcaact aagcggatac ctacaactaa gcttgtaact gtagcaatca
     6541 tttttgcatt cgttgcctta tctgttcctg cgaacatctg gcttgtcacg aataccataa
     6601 aataagtgct taatgtaaca tagaatgtgt cgtgtccaaa agcgcctaaa gcgtaggaca
     6661 cacgttggat cattttgctt ttcataatct gtattcctct cctgttcaat atataatact
     6721 aagtaaaccg gttacaacga ataacaaaga tcttttaatg acttaaactt aacaagaaat
     6781 cgcttccttt ttaatatatt tctgtgactt ttatttaact ttttgttgca ttatactaaa
     6841 ggagtaagga gggatagacg tggagcaatc gattttttta aaaaaggaac atcaacaagt
     6901 agaaaaagaa ttgtattttg atttttgtgg tttctctaaa acgatgccgt ttcattcttt
     6961 tggtcctgct attcgcaatg attacatcct gcatgtcgtg atggaaggca aaggatctta
     7021 tcatgtgaaa aatcaacaat tccaattgaa aaaaggtgat ctgttcttga ttcgtccagg
     7081 agactcgacg ttttacttat cagatggaga agatccgtgg atgtattgct ggctttcatt
     7141 tggtgggagt gctgcagaag aaatcattcg gcagtcgctt ttcagagaag accaatacac
     7201 gatggtctct tctgacatac aaaaatatat cgacattatt ttagaatgca tgaaagatac
     7261 ggaagaaacc ttggaaaatg aattacagtt aacagcatta acctaccgtt ttttatcagt
     7321 ccttttaaaa gatggcggaa aggtcagcgg tggtgtacaa aaggttcatt cctctttagc
     7381 tgttgaaaca gtcaaataca tcgaggcaca ttatgctgaa aaattgtctg ttgaggaaat
     7441 cgcccagcat ttagcggtca atcggagcca tttgtcgcga gtattcaaaa atcatatggg
     7501 taccagtatc aaggaatatt tgatcggggt acggatcaat cgaggggcgt ttcttctttc
     7561 tctgacggat gaatccgtgg aaagtattgc ctatcaagta gggttcaata gtctagtcgt
     7621 atttagccga atgttcaaaa agagtaccgg agaaacggct acgaactatc gtaaacgtat
     7681 gaaaaacgaa aattttaaga atattgcttt ggataccttg aaaaaacagt tggaaaaaca
     7741 aaccgcagtt tcttggacaa cctgattagt ctggttaaaa tgacaagcat cggcaaaaga
     7801 aacttgtggc ggttatgcta cactgtaaag cgaagactaa aaaggaggaa aagggatgcg
     7861 tacacttatc atcattagcc atccaacgat cgaagaatct ttaaatcagc aatttttcaa
     7921 agaagcttct gctggtttac cagctacttg gcatcatttg gaatcaagtt atccagacgg
     7981 aaaaatagat atagagactg aactacagct cttggcccag catgatcgga tcattttcca
     8041 gtttccgttt tactggtata gttctcctgc tcatttaaaa atttggcaag ataatgtctt
     8101 ggaacgagca gcacatgtat taaaagggaa agaactagga atcgtattga caacaggtgt
     8161 ggcggaaaaa gaataccaag ctggaggaaa agaagaatat acgatttccg aatttttgag
     8221 gccgtatcag cgaatcgcaa ataaattcca tatgacctat cttccgccat tcgttttagc
     8281 acaatttatg tatttatccc aggaaaaacg ttgggagaag ctaattgctt atcagcagta
     8341 tctctcattg gaaggcaaac catcgctgac tcagcggatt gactggttta ttcaaagagt
     8401 acaggaaaat cagaaaatgc aggaagaaga atctgaaaaa caagcatata tcgtagaagc
     8461 actgactgag gcaaaagagc agatcgaaga cttgaccttt acactgcaag aaatgaaagg
     8521 aacttcatta tgacaaatga ttggctgatc caacaattag atgaattaag cgaaaaatca
     8581 gaatatcaag accgtgcgct cttatatgaa ataaagaaac tgatggctga gatagacgaa
     8641 cgaacaatgc agctgcaagg agaaatcgac ggacaatctt ggaatcatct caactggtga
     8701 aattttctta taaataacaa agataaaaat gtgcacagcg acaattattt gctacaataa
     8761 gaatatgaag cgtttgataa cgctattata cataaaaatt ggtggtgaaa aaaatgtatg
     8821 atgaaaatga gtggggagaa aatgttcgga tgacccgaag aagacagcaa aaacaggcag
     8881 ctcgtaagcg gatgatcatg attggggcag cagttgccgt gtttttgatc atagcaggag
     8941 gagttgcggg tctatatttt catggaaaaa attcgtcaga agcgaaaagt acgaccataa
     9001 cggaaacaac gacaaagaaa acagagacga aacaaacaag tgagtcatca actaaaacaa
     9061 aagagacaca aacttctgaa gaaaaaacag caaatggttt gccagagcaa cttcaagcaa
     9121 tcgtaaatga ttattcgaaa aagatcgaag aaaagacgcc tgcattgatt gaagaatacc
     9181 agacagaaat tcagggaaat caagagggaa tagctggttt atcagcagtt gctaaccaaa
     9241 aagcacgtga gcttcaagca atctccgacg aagggatcag aaaattacgt gcagcgtatc
     9301 aggcagcaga aaataaagac ggtgtagact tagatacttt gatcaatcaa ttatcggcta
     9361 attatacgaa tcatgtcgct aagatttccg atatttattt gcagactagc gcctctcttc
     9421 aagcagagtc aacaagcaca caagacacaa catcttctga ttctgagaca gcagagtcgg
     9481 tagaagcttc tgaaatgcaa gctcgaacaa cacaagacac aacagattcc tctgaaacta
     9541 ataccaacag cgcgacttca caggctgcga caaccgtagt tttggaagga gaaggtccaa
     9601 atcaaatagc cgaacgaact ggcgtaccag tagaaacgat cttgtcgctg aatgggatga
     9661 gcatggagaa ttatttcctg actcccggtg agacgctaaa attgaattga taaaagcaat
     9721 aaaaaagaac gaagcttcca ctaaggtgaa gcttcgttct ttttttattt tccggttctt
     9781 ttttcgcgta caaagtaatg gatcgtcatg taaagcaaga aaacgagtcc aataatcaat
     9841 gagattctcg tttcaggatt gataaacatg aaaatcaata cgatagctaa aaaagcaagt
     9901 gtcacataat tcgtataagg agatagtggc attttaaaag gatgatcatg cattttatct
     9961 gcgtgcttct ttttgaagcg aatctcgctg atcaaaatca ctacccaagg aaccatacca
    10021 ggcaaaatac ttgagctgta tacgaaaacg aaaatatttt ctgactggtt gatgaacatt
    10081 ggtagaagat aatttaagat cagaccaatt aagattccac ctgagattgc tacgacagga
    10141 taaaaaggcg caccgtgacg agatagtttc agaaattttt tagacatctt tccatttatg
    10201 gcaagtgtat agaccatacg acttgcgctg aaaatccctg aattgcaacc tgataatgcc
    10261 gctgtaatca cgacaaaatt aatcagtcct gcagcaaatg taatgccgac tttagaaaaa
    10321 gtttgtacaa aaggtgaacc aatctcactc aattgattcc acggatagat actcacaatg
    10381 ataaaaatag caccaatata aaaaatcaag attcgaccga tagtggactg gacagcttca
    10441 atgatcgtct ttttaggatt ctctgcttca ccagcagtca ttccgatcag ttcgattcct
    10501 tgataggaag caacaacgat cgataatgca aagaaaaatc ctttgacgcc tccagtgaag
    10561 aatccaccat ttttccaaag atttgaaatc cctactgcct gaccatgatt accaaatcca
    10621 aataagatta ccccaagacc tgcaatgatc atcaaaatga tcgttatgac tttgatgatc
    10681 gaaaaccaaa attctaattc gccaaacgcc ttgacagaaa taagatttgc cagcataaga
    10741 aacaagacga caatgattcc gggaataatt gccggcaaat caggccacca ataattcata
    10801 taagatccta cagcaatcac ttcacttatt cctacgacaa tatattgaaa aatattactc
    10861 caagcggtca agtagccagc cacaggatgg atgtactcgc tcgcgtaatt tgcaaaagaa
    10921 ccagtcgaag gatcaacgta aagcatttcg cctaaagcac gcataatcaa atacaaaaag
    10981 aatcctgcaa tcgcataggc tcccatcact gagggccctg tccaacgtat ggttgaggag
    11041 gcacccataa acagaccgac accgatcgtt ccacctagtg cgatcatttg gatatgacgg
    11101 gcactaagac ttcgagttaa cttttcttct tccatacaaa aactcctttc gttgttgagt
    11161 caacaagaaa aaataatcaa acgtttcaac acatgttttt cattattagg ttagccttat
    11221 tgaactcttg atgtgattct acagaaaatt ttcataaaat gcgaggaaaa ttcgaaaaaa
    11281 actttttcat cattctactt gaaaatgctt tccattttag ttaaactata tttaaataaa
    11341 aagttaggta aacctaaaaa ggagtgtaag atttggaacg tacggccatt gtaattcaag
    11401 acctttcagc cgcttatcaa gggaaaactg ttttgaaaac tatcaacttg acgataaatc
    11461 cacaaaaaat taccggtatt atcggaccaa acggtgcagg aaaatcaaca tttatgaaat
    11521 ctttgctaga gttgatacca gctactactg gtaaagtcac cttttctgga cagccggtaa
    11581 actcagtccg taaaaaaata gcctacgtgg aacagcgaag tgaattggat ctttcctttc
    11641 caattgatgt attaggcgtt gtacttttag gaacatatcc atctttacga attggacaaa
    11701 gacctgggaa aaaagaaaaa gaacgtgcaa gacaagcttt gaaaaaagta gggatggaag
    11761 aatatgccaa aagacagatc agcgaactat cgggtggaca gcttcagaga gtttttattg
    11821 caagagctct agcccaagga gcagaatgga tctttttaga tgaaccattc gtagggattg
    11881 atgcgttaag tgaacgaaag atctttgaca tcttgcagga attgaagaat tcaggaaaaa
    11941 cgattttgat cgtccatcat gatcttcata aagtagaaga atatttcgat gaggttattc
    12001 ttttaaataa acagctgatc gcttccggtc cagtacaaga agtctttaca tcgaagaacc
    12061 ttcaattggc ctatggtgag atcatcagac atttagtaaa aggaggagag aaaaaatgat
    12121 ccaatccttt attgatggat taatgaatta tcaattcctg caatacgctt tggtcacttc
    12181 catgcttgta ggacttgctt ctggagtcat cggttctttt attattttac gagggatgtc
    12241 tttgatggga gacgctatct ctcacgcagt tcttccaggt gtagccattt cttatatgtt
    12301 tggttttagt tacatctttg gagccactgc ttttgggatg ctaacagcag cagtgattgg
    12361 tttcgtgact cagcggagcc gattgaaaaa tgatacagcg attggaattg tctttagctc
    12421 cttttttgca ttagggatta tcttgatttc atatgcccag agtgcgacag atttgtatca
    12481 tattttattt gggaatgtct tagctgttag ggaaagcgat ttgttgctga cagcactcgt
    12541 tagtggaatc gtcttgatat ttgtcttttt cttttataaa gaattgaaga tcacatcatt
    12601 cgatccgaca atggcaaaag cttatgggct gaacacatcg ctgatccatt atcttttgat
    12661 gttctttttg acattagtgg ctgtagtcag tttacagaca gtaggaacaa tcttggtgat
    12721 tgccatgttg atcacaccag ccgccacggc ttacttgcta acgaaccatt tactgaaaat
    12781 gatcattaca gctgcaggaa tcggtatgct aagtgcagtt gtcggtgtgt ttttcagtta
    12841 tagttacaat tggccatcag gagctacgat cgtgttagca tgtaccgcat tttttatcct
    12901 tgctttttta ttttctccaa caaaaggaat tttatttcag aaaggaagca gatagatgaa
    12961 aacaaaaaaa tcactctttt taatcttggc agttagtttc ttggtattag ccggttgcgg
    13021 aaagcaggct tctgaccaag cggatgaagg aagtaaagaa aagttgtcag tcgtggctac
    13081 caattcgatc ttggcggaca tggcaaaaga ggtcggaaca gatcaaatag atatccacag
    13141 tatcgtacca gtcggaacag atccgcatga atatgaagta ctaccagaag acatcaaaaa
    13201 ggcaagcgat gctgatgtta ttttatacaa cggtttgaat cttgaaacag gtaacagctg
    13261 gttcgataac ttgatggaaa cggctaaaaa agaagaagga aaagattatt ttgcagttag
    13321 caaaaatgta gaacctcttt atttaactag cggtgaagaa catacaaaag cagatcccca
    13381 tgcttggcta gacctatcta acggaataaa atatgtggag gaaatcgcac gtatattctc
    13441 tgaaaaagat gcagaaaatg cgacactcta taaaaaaaat gcagaagcat atgtggaaaa
    13501 actaaaagaa ttagatacac aagcgaagga aagttttgct tctatcgaag agaacaaaaa
    13561 attattagta acaagtgaag gggcattcaa gtatttttca cgagcatatg atttgccagc
    13621 agcttatata tgggagatca atacagaaag tcaaggtacg cctgatcaaa tgaaagcgat
    13681 cattgatcag ataagagcaa aagaagtacc ggttttattc gtggaaacca gtgtagactc
    13741 aagaagcatg gaacgggtag caaaagaaac gggtttgaaa atctatgata aactgttcac
    13801 tgattccata gcaaaagaag gagaacaagg agattcttac tatcagatga tgaaatggaa
    13861 tattgaaacg atccatgaag gtttgtcaca aacaaaagaa agctgaaaag tgcaaggtct
    13921 agcttgagga gcagaccttg cattttttat aagagatatg aaaaaaaatt atgctgtttt
    13981 tcgtcgtaca gatacctttt ttgaagaaat tccgttatag ttagagtaag gaaggaaggt
    14041 gtggctatga attttacacg taaagataaa tatacgatta aagaagaagt ctttcgattg
    14101 atcagccagc aggaaaatac cgacaaggat caagaccttc aatttcttga agcagaatta
    14161 gctaaattat tcatagcaaa tcaaaatgag acaacagaac cagatgaaga gctgtacata
    14221 ggcaaatggt tgtttgatga atttcagcct ttagatgaaa ataccggtat cggttatttt
    14281 gtgttccttt ctccagtcgg cttcacacct ggcgtattag ttaaacaaaa cgacgaaata
    14341 accgtcaaag catggcctgc attattttca gtcatcaaag cagaaggagt aatcaaaaag
    14401 atccagtcgg cctattataa aagtcaattt attgccaaca gcaaattaat gaatatgttt
    14461 tataatcgtc tagtagaaat tgctaggcct gggaaaatcc cgcaaacatt cacgtacaca
    14521 cagcaaaaaa atctctatga agaagtattg tattcccgcg atctgtctga gttttcgcca
    14581 tgggaagagg taaggctcca tgaactgtta agtacaaaac cagtttcatt gaattttttt
    14641 aatgcattag ataacaaaag aaaattaatt tcaacaaatt attgggaagt atttgctgca
    14701 agaaaaaaat ggatggaaga aaagaagatt gccgatcagc agatccagtt tgtccagctg
    14761 aaaaaagaaa aactatatta tgggttatac agccgttaac agcaagcgag gaagaccatg
    14821 acattaaaag aagaaatcat acaagaaagc aaacgattag gaattgacaa aatcgggttc
    14881 accacggcgg aaccttttga ttcattaaaa gaatcgttgg aagaacaaaa agcagctggc
    14941 catacttctg gctttgaaca tccaaatatc gatgagcgtc tttatccaga aatgacattt
    15001 gatcagccga aatcgatcat ttccattgca cttgcgtatc ctactaggat ccatgaagaa
    15061 gtgcctaggg atgaaaaacg cgggcagttt gctcgtgctt cttgggggat cgattaccat
    15121 gatatactca gagaccgttt gaaccgattg atcgaattca tccaaacaag agcagaaaaa
    15181 tggcaaaaag aagaggaatg gcgtctagct ccgcaagtag atactggaga actgatcgat
    15241 gtcgctgtag cacagcgagc tggtttagga tttattggac gaaacggatt gttgatcact
    15301 gaagagttcg gttcatttgt ctatcttggt gagatcgtta ctaatattgt atttgagcct
    15361 gataaaccag gagaatttgg ttgcggtgac tgtatgcgct gcgtgactgc ttgtccgaca
    15421 aaagcattgc ttggtgacgg aagaatgaat gcccagcgat gtttgtctta tcaaactcag
    15481 acaaaaggga tgatgccgga agaatatcgt aaaaaaatgc gaaatgtcat ttatggatgt
    15541 gatatctgcc agttagtctg cccttacaat caaggaaaag attttcattt tcatgaagag
    15601 atggaaccaa aagtagacga agtatatcca aaattaaagc caatgctttc gatgtctaat
    15661 aaagacttca aacagcaatt tgggcattta gctggttcat ggcgtgggaa gaaacctttg
    15721 cagagaaacg cattgattgc tttagctaat ctaggtgatc gaacggcatt gcctgagatt
    15781 tcagaatgtt taacagatgt ccgccctgtc atcagaggaa cagcagcttg ggctattgga
    15841 cgtctaggaa caaaagaacc ggaaaaatgg cttgatgtat tagatgaaat attagctaaa
    15901 gaaacagaag aagtcgttat aaacgaattg gaacatgccc agaaattatt gcggagaaag
    15961 ttaaaataaa aaaatagtta acattattca aagtttttgt ccagtaattg ttgtaacaat
    16021 tactggtttt tttgtatttt ttacattttt ttaagagaat ttgataaaaa tcaaaaattt
    16081 ttttgctttt ttttatggat tattgc
//
LOCUS       DAJSBL010000061        14939 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.063, whole genome shotgun sequence.
ACCESSION   DAJSBL010000061 DAJSBL010000000
VERSION     DAJSBL010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14939
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.063"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..562
                     /locus_tag="ONT85_002296"
     CDS             <1..562
                     /locus_tag="ONT85_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HCR2379249.1"
                     /translation="LKNEFGIEVKDYFIDLDLLQLINSVKRMYSGDDNGVDLYKFVTK
                     HIFVNLNIDEKNYPSIFKHYLFFINEVEDRIKNNSLSSSVSSFKKMFKSRDGITLTTA
                     HKVKGNEYDTVIAINLLNGKIPHWNEIFNVSDKGISSARKLLYVICSRAKNNLFLFSE
                     VGYFTSKGDLLYPTQLLDKIEFDYNI"
     gene            762..1076
                     /locus_tag="ONT85_002297"
     CDS             762..1076
                     /locus_tag="ONT85_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HCR2379250.1"
                     /translation="MELKYVIPNMEKTFGQLEYAGEGNIEQRRINGRPTILSRSYNLY
                     STIQRADDIVVILPSEAGEKHFESDEKVKLINPHITAEGYKIGNRGFTNYIMHADDMV
                     KA"
     gene            1097..1474
                     /locus_tag="ONT85_002298"
     CDS             1097..1474
                     /locus_tag="ONT85_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HCR2379251.1"
                     /translation="MRLAEGIVIEKDQTFGTLKFSALRREVRIQNEDGTVSEEIKERT
                     YDLKSKGQGRMIQVSIPASVPLKEFDYNAEVELINPVADTVATATFRGADVDWYVKAD
                     DLVLKGQTKPHQKYKKEASESEK"
     gene            1484..2257
                     /locus_tag="ONT85_002299"
     CDS             1484..2257
                     /locus_tag="ONT85_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2379252.1"
                     /translation="MTNTPLILKEIPKDEAISFIRQYHYSKILPRLCKYFLGIFSEEK
                     LLGVVELGWGTQPLQTIRKLFPDSSLQTTDYLEIGKMCFLPEMNQTNYFGSQALSALI
                     KWLKEHTDCHFLYTLADGIEGKCGYVYQASNFFYCGYFKTSVYRDKQSWEKIHPRSAR
                     LLLEENARFEQVEKKHWLSQAFCEYKGIEKINGRMFRYLYPLTKEAKKLLGHTLYRRH
                     YYPKEKNLRFEKRIAYQKYEAISQPTFDKQARIYNTQLF"
     gene            2279..3682
                     /locus_tag="ONT85_002300"
     CDS             2279..3682
                     /locus_tag="ONT85_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HCR2379253.1"
                     /translation="MPLLHDRGERIKANDKHLVYHFVLISLLVVFLVVLLPFHWRALQ
                     SVNWQAVQLDTFFYQLHTPYFWSSILTALFVCGLISWLYYHYRIDSIKQLEHRQKLAR
                     MILENHWYEAEETQSEGFFKDLPSKKTKQRIRHFPKIYYRMKDGLIHIHVEITLGKYQ
                     DQLLHLENKLETGLYCELVEKTLKDSYVEYVLLYDTIGNRIGIEDVVAQDGRVKLMES
                     VYWEFDSLPHMLIAGGTGSGKSYFILTLIESLLHTNAKLYILDPKNADLADLGAVMNN
                     VYYRKEDMLACINQFYEDMIDRSETMKQHPNYKTGENYAYLGLSANFLIFDEYVAMMD
                     MLGRESTTVIHKLKQIVMLGRQAGFFLILACQRPDAKYFSDGIRDNFNFRVALGRMSE
                     MGFGMMFGSDTQKQFFLKPIKGRGYVDTGKSVISEFYTPLVPKRYDFLGEIGKVIQKK
                     QSEPVPHEVKGTGSDLL"
     gene            3864..5048
                     /locus_tag="ONT85_002301"
     CDS             3864..5048
                     /locus_tag="ONT85_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000426690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HCR2379254.1"
                     /translation="MENEKIWIKELKEKRLAYGVSQNKLAVASHITRPYLSDIETGKA
                     VPTQQVKEDLLNALERFNPDNPLEMLFDYVRIRFPTNDVAQIIGEVLRLNMDYMLHED
                     FGYYSYPEHYRFGDIVVLVSHDVSKGVLLELKGKGCRQFENFLLAQHRSWYDFFQDCM
                     EHKGIFKRLDLAINDKTGILNIPDLAKKCKQEECISVFRSFKNYRSGELVHRDEKPYM
                     GNTLYIGSLKSEVYFCIYEKDYEQYVKNDVPLEDAEVKNRFEIRLKNDRATQAMKDLL
                     AHQQAEKTAFEIINRYIRFADKDDTKRRSDWKTNERWEWFIGKNRGALRLTTQPEPYS
                     FERTLNWLHHQVAPTLKIASILDVLNGTTIISTMIQEAKLTEKHEKLIEQQHLAMEDL
                     IT"
     gene            5045..5335
                     /locus_tag="ONT85_002302"
     CDS             5045..5335
                     /locus_tag="ONT85_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000055367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2379255.1"
                     /translation="MSLLTKELKKLGFQCGIEFQAYIQNTGKYTSLIIEGKRQAGDTI
                     YTYDFYKVRFYNNYTNRVTVYGEHLTPFQLLRRVKSYIYYREKYLKERRTIT"
     gene            5332..5553
                     /locus_tag="ONT85_002303"
     CDS             5332..5553
                     /locus_tag="ONT85_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2379256.1"
                     /translation="MNFGQNLYNWFLDNAQSLVLLAIVVIGLFLGFKREFSKLIGFLI
                     IALIAVGLVFNAAGVKDVLLNLFNRIIGA"
     gene            5595..6374
                     /locus_tag="ONT85_002304"
     CDS             5595..6374
                     /locus_tag="ONT85_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000678782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection family protein"
                     /protein_id="HCR2379257.1"
                     /translation="MKGFMNMFTNDQFKSLLIKTLERKSSSNYYEGGNEIVSKMKISE
                     VTLDETDDFTYYKGYKKGWNTLCTYLKIRVPFDDLDFFESHKDLITQTASSIYDKQGD
                     NVLVDTILVPLPENYEVINFSQLKISDVVSQAIEDAESFMSNGEYQRAFDRVHTAFHG
                     YLIEILKKYEITVPRDENLSKLYSRIQQLIEKEIQPTELADIVKTTIRSSNGMISSLN
                     EARNRHSLAHPNTNIIGKREAKLIIGISSTVTDYISGYLDK"
     gene            6441..6935
                     /locus_tag="ONT85_002305"
     CDS             6441..6935
                     /locus_tag="ONT85_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000870467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HCR2379258.1"
                     /translation="MKVYVANLGRYNEGELVGAWFTPPIDEEEMAERIGLNEDYEEYA
                     IHDFELPFDVDEYTPISEINRLCEAIQEIEGTPIYNELKEIQGMWFSSLEELLENKED
                     IHCYSDCDSMEDVARYYVEETGQLGEVPSNLQNYIDYQSLGRDMEIEGNYLVTSHGVF
                     EYCQ"
     gene            6991..7629
                     /locus_tag="ONT85_002306"
     CDS             6991..7629
                     /locus_tag="ONT85_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2379259.1"
                     /translation="MVNVFENLKLLKLDMKDKGWVIDSFYFRYKQQNYIVLVKLFEKE
                     EKVPEYALLKLEFLKENDFSDMLAVYANSVKLYTDTKTIREYFGIEYSSNLGDVLFQF
                     SQTLARFIPTEVSEKKNEDQKEAMCFSLSQSDSEDPRKKYCFSVRRNPLKGNGELGKR
                     SPFNDNKTRLYRPSLYERLGSDTNLSFRYSMNPDDEETDEGIIAKWTKNKIE"
     gene            7701..8096
                     /locus_tag="ONT85_002307"
     CDS             7701..8096
                     /locus_tag="ONT85_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HCR2379260.1"
                     /translation="MKKIRSYTSIWSVEKVIYAINDLQLPFPITFTQMTWFIVSLFVV
                     ILFGSLPPLSLIDGALLKYLGIPIVLTGFMCQKTFDGKKPYGFLKSIITYALRPKVTY
                     AGKQIRLKKETMNESITIVRSEQLDGISD"
     gene            8080..10533
                     /locus_tag="ONT85_002308"
     CDS             8080..10533
                     /locus_tag="ONT85_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCR2379261.1"
                     /translation="MAYPIKYMEDNLVFNHDGEVFAYYELIPYNYSFLSSDEKIQVHD
                     NFCQLIAQNRDGKIHALQLATESSIRATQEQSKKEITGRLKEIAYEKIDEQTEALISM
                     IGDNQVDYRFFIGFKLYLNEQEVSVKNVGKEIQKAFAEFFHGVNHKLMGDFVSMPMAE
                     IERFSKMEQLLESKLSRRFQIRKLEKDDFGYIIEHLYGQTGVPFDDYDYHLPKRYEDK
                     KVLVKKYDILKPTRCLIEENQRYLRIEQEDTTTYVAYFTINSIVGELDFPNSEIFYYQ
                     QQQFTFPVDTSMNVEIVANKKALSTVRNKKKELKDLDSHAWESDNETSTNVVDALESV
                     DELESNLDQTKESMYKLSYVVRVSAKNLDELKQRCDEVKDFYDDLSIKLVRPFGDMLG
                     LHNEFIPSSKRYINDYIQYVTSDFLAGLGFGATQMIGENSGIYVGYNLDTGRNIYLQP
                     SLASQGIKGSVTNALASAFIGSLGGGKSFSNNMLVYYSVLFGGQATIVDPKAERGEWK
                     ETLPDIAHEINIVNLTSENDNKGLLDPYVILKLPKDSESLAIDILTFLTGISSRDSEK
                     FPVLRKAIRSVTQSKQRGLLLVIDELRKEDTPISNSIADHIESFVDYDFAHLLFSDGS
                     VTQSISLEKQLNIIQVADLVLPDSNSSFEEYTTMELLSVAMLIVISTFALDFIHSDRS
                     IFKIVDLDEAWSFLQVAQGKALSMRLVRAGRAMNAGVYFVTQNADDLLDEKMKNNIGL
                     KFAFRSTDLVEIKKTLEFFGVDKEDENNQKRLRELENGQCLVSDIYGRVGVMQFHPIF
                     EELLHAFDTRPPVRNEVEE"
     gene            10530..12557
                     /locus_tag="ONT85_002309"
     CDS             10530..12557
                     /locus_tag="ONT85_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2379262.1"
                     /translation="MTQKQKKFLHYSWITLLCVGVILLLLGTLGNAVQATGLVDETID
                     TSNEYSKYGLNQYQLDYYVDNSWGWLPWNWSDGIGQSVMYGLYAITNFIWTISLYLSN
                     ATGYLVKEAYALDFISQTSQAIGENMQTIAGITPNGFSSSGFYVGFLLLFILIVGIYV
                     AYTGLVKRETTKVIRAVLNFVVVFILSASFIAYAPDYIAKINDFSKDVSTASLNIGTK
                     IVLPNTDSQGKDSVDMIRDSLFSVQVKQPWLLLQYGSTDVEALGEERVESLLSTSPDT
                     NNGEDRENVVIEEIEDHDNTYLTLPKTISRLGTVFFLFVFNIGISIFVFLLTGIMIFS
                     QVLFIIFAMFLPISFLLSMLPSFDGMGRHAITKLFNVIMTRAGITLIITVAFSISTML
                     YSLSASSPFFMIMFLQIVTFAGIYFKLGDLMSLFTLQSNDSQNMGRQVFRRPRQMMNR
                     QTRRFQRSMSKIFRGKAGSTKQKTMTTKQNQTNKANHTRQNERTTPKKETALSNVKKQ
                     QGGIGQRLGEKTAHVLDTKNRLVNKRKQAKQQIQATPTHVKYALHQGKENVTHNVTDF
                     KQSMADTKAQRKQQRMNQQEQKRKNIAQKRLELDKAKEKRRKENPSSPVQNNQQHVRK
                     RQQTVTPVLSQNTKRENHQRPLKKEQKQKTPPKQFNQRKLNKRTDKRGQQT"
     gene            12554..13576
                     /locus_tag="ONT85_002310"
     CDS             12554..13576
                     /locus_tag="ONT85_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HCR2379263.1"
                     /translation="MKLKITVICSVVFSLFFFLLLFLVIFFADDTDSGENNKDSSIPQ
                     GGVTVSPEVLAHRPLIEKYGKEYGIEDYVSYILAIMQVESGGTAEDVMQSSESLGLPP
                     NSLSTEESIKQGVKYFSELLTSAEQQGVDIDSVIQSYNYGGGFLNYVRSHGKKYTYEL
                     AEQFSKEKSGGQKADYPNPIAIPVNGGWRYNYGNQFYVQLVSQYLTDTSPTEFDDETV
                     QVIMDEALKYEGFPYVFGGASPTTSFDCSGLIQWVYDKAGISLPRVAQDQYDATQEIS
                     MEEAQAGDLIFFHSTYNAGTYVTHVAIYLEGNRFYHAGDPIGYGDLSSRYWQDHLIGA
                     RRVIHN"
     gene            13593..14522
                     /locus_tag="ONT85_002311"
     CDS             13593..14522
                     /locus_tag="ONT85_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HCR2379264.1"
                     /translation="MIIKIERKEKKEKPKKSKKPSKQRKVPMIKVGTHKKLTFVLWVL
                     LIGSVGFGIYKNFTAIDTHTVRETEIIKQQIVDTNQVESFVKSFAKDYFSWQQSQEAI
                     DKRNEKLTHYLTEELQVLNEEMIRKDIPTSSSVNDIQVWQVSQVNENTFEVLFSVEQV
                     ITEDKDKETISSSFHVVVHIDESDNMVIIKNPTMSKKPQKSDYQPKQLESDHTVDTET
                     MDEITSFLETFFQLYPTATEKELTYYVSNHVLPMINKEYVFEELVNPIFTRKDNQVIV
                     NVAVKYLDQETKATQISQFELILEKQDNWKIVK"
     gene            14767..14883
                     /locus_tag="ONT85_002312"
     CDS             14767..14883
                     /locus_tag="ONT85_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HCR2379265.1"
                     /translation="MILKYPENICMLCIPMVMHKNPSDKSIYHWDFYALLGF"
BASE COUNT         5072 a         2485 c         2954 g         4428 t
ORIGIN      
        1 attaaaaaat gaatttggaa tagaagttaa agattatttt attgacttag atttattaca
       61 gctaataaac agtgtgaaaa gaatgtatag tggagacgac aacggtgttg atttatacaa
      121 atttgttacc aaacatatat ttgttaatct taatattgac gagaaaaatt acccgtctat
      181 ttttaagcac tatctatttt ttattaacga agtagaagat cggataaaaa ataattcatt
      241 atctagtagt gtatctagtt ttaagaagat gtttaaatct cgagatggta tcacacttac
      301 tacagcacat aaagttaaag gaaatgagta tgatactgtt atcgctatta atcttctaaa
      361 cgggaaaatt cctcattgga atgaaatttt taatgtttct gataaaggaa ttagttcagc
      421 gagaaaacta ctatacgtaa tttgttcaag agcaaaaaat aatttatttc ttttctcaga
      481 ggtgggttat tttacgagta aaggagactt gctttatccc actcaattat tagataaaat
      541 agaatttgat tataatattt aaaataccta ggtagtcgtt gttttaaata tagtaggaat
      601 cggacgctca gtacggagcg tctatttttt tacccaattt tgaaaggaag tgaacttatg
      661 aaaataaaaa atcaagaatc aaaaggtcgc tctcccccct ttaaaaccat cacacaaata
      721 agattcattc aaacataaaa acagaaagga taggtaaaaa tatggaactt aaatatgtca
      781 ttcccaatat ggaaaaaaca tttggacaat tagaatatgc tggtgaaggg aacattgaac
      841 aacgtcgaat aaatggcaga ccaaccattc tctctcgtag ttacaattta tactcaacga
      901 ttcaacgagc agatgatatt gttgtcattc ttccctccga agctggcgag aaacattttg
      961 aatccgacga aaaagtcaaa ctcatcaacc cacatattac cgcagaaggt tataaaattg
     1021 gcaatcgagg atttaccaat tacattatgc acgcagacga tatggtgaaa gcataagaaa
     1081 ggaaaggtga aaaaatatga gattagcaga aggtattgtg attgaaaaag accagacatt
     1141 tggcacattg aaattttccg ccttgcgtcg tgaggttcgt atccagaatg aagatggtac
     1201 ggtttccgaa gaaattaaag aacgtaccta tgacttaaaa tcaaaaggac aaggacgcat
     1261 gatacaagtc agtatccccg cttctgttcc tttgaaagaa tttgactaca acgcagaagt
     1321 ggaattaatc aatcctgtgg cagataccgt agcgacggct acttttcgag gggcagacgt
     1381 tgattggtac gtgaaagcag acgatcttgt attaaaagga cagacaaaac ctcatcagaa
     1441 atataaaaaa gaagcgtccg aaagtgaaaa ataggtgttg cttatgacaa acacaccatt
     1501 gattttgaaa gaaattccga aggatgaagc catttctttt attcggcaat accattattc
     1561 aaagatactc cctagattgt gtaaatactt tctagggatt ttttcagaag aaaaattatt
     1621 aggcgttgtg gaattaggct ggggtacgca acccctacaa accatacgca aactctttcc
     1681 cgacagttcc cttcaaacca cagattattt agaaattgga aagatgtgtt ttcttccaga
     1741 aatgaatcaa accaattatt ttggcagtca agctctttca gctctaatta agtggttgaa
     1801 agaacatacg gactgtcatt ttttatatac actggcagat ggaattgagg gaaaatgtgg
     1861 ctatgtctat caagcgtcca acttctttta ctgcgggtat ttcaagacaa gtgtctatcg
     1921 tgataagcag tcatgggaaa agatacaccc tcgaagtgct cgccttttat tagaggaaaa
     1981 tgctcgcttt gaacaggtag agaaaaagca ttggctttcc caggcatttt gcgaatacaa
     2041 agggattgag aaaatcaatg gacgcatgtt tcgctatctc tatccgttga caaaagaagc
     2101 gaaaaaactg ttaggacaca ctctctatcg acgtcattat tatccgaaag aaaaaaattt
     2161 acgctttgaa aagcgaatcg cttatcagaa atatgaagcc atttctcaac caacttttga
     2221 taaacaggct cgtatctaca atacacaatt attttgatag gtggaaagga ggatttttat
     2281 gccattgctt catgacagag gagagcgaat caaagccaac gataagcact tggtctatca
     2341 ttttgtacta atttcattgc tggtagtttt tctagtcgtc ctactccctt ttcattggag
     2401 agcgttacaa tcggtgaact ggcaagcagt tcagttagat acatttttct atcaacttca
     2461 tacgccatac ttttggagca gtattctcac tgctcttttt gtatgcggac ttatcagctg
     2521 gctttattat cactatcgta tagattctat aaaacagtta gagcaccgtc aaaagttagc
     2581 tcgtatgatt ttagaaaatc attggtatga agccgaagaa actcaatcag agggattctt
     2641 taaagattta cccagcaaga aaaccaaaca acgaatccgc cattttccta aaatatatta
     2701 tcgaatgaaa gatggactaa tccatattca tgtagaaatc accttaggga aatatcaaga
     2761 tcagcttttg cacctagaaa acaaactaga aacaggattg tattgtgaat tagtggaaaa
     2821 aacgttgaaa gattcttatg tagagtatgt cctactttat gatactattg gcaatcgaat
     2881 tggcattgaa gacgtggtag ctcaagatgg acgtgtcaaa ttgatggaat cggtctattg
     2941 ggagttcgat tctctccctc acatgttaat tgctgggggc actggtagtg gaaaatccta
     3001 ttttattctc acattgattg aatctctcct gcatacaaat gcaaagcttt atattcttga
     3061 ccctaaaaat gctgatttgg cagacttagg ggcggtcatg aataatgtct attaccgtaa
     3121 agaagatatg ttggcatgta tcaatcaatt ttacgaagat atgattgatc gcagtgaaac
     3181 catgaaacaa caccctaact ataaaacagg agaaaattat gcttatcttg gtttgtctgc
     3241 caattttctt atttttgatg aatatgtggc aatgatggat atgctaggtc gtgaaagtac
     3301 caccgttatt cataaactca aacaaattgt aatgttagga cgacaagcag gcttcttttt
     3361 aattcttgcc tgtcaacgtc cagacgcaaa atattttagt gatgggattc gggacaactt
     3421 taacttccgt gtggctttgg gtcgtatgtc tgaaatgggc tttggtatga tgttcggaag
     3481 cgatacacaa aaacaatttt tcttaaaacc catcaaggga agaggttacg tggatacagg
     3541 aaaaagtgtg attagtgagt tttatacacc ccttgtacca aaaaggtacg atttcttagg
     3601 agaaatagga aaagtcattc aaaaaaagca atctgagcct gtaccgcacg aagtgaaagg
     3661 tacaggttca gacttgcttt gattggtgtg gcgttagcca cgccaatcct ttagccccct
     3721 ctatctaaca ggggggcaca aatacaactg aaaacaggca attctcatca accaatgttg
     3781 atggtacaaa ggattgcgga aaatatgaaa ggtgtaaact caagggtcat agtttacatc
     3841 ttttttagat tggaggtttg cgaatggaaa acgaaaaaat ttggattaag gaattgaaag
     3901 aaaagcgact ggcttatggc gtttctcaaa ataaactggc ggttgcttct catattacca
     3961 gaccttacct atcggatatt gaaacaggca aggcagtccc tactcaacag gtgaaagaag
     4021 atttactgaa cgctttggaa cgcttcaatc ccgataaccc attggagatg ttattcgact
     4081 atgtacggat tcgattccct accaatgatg tggctcaaat tatcggtgag gttttacgtt
     4141 tgaatatgga ttatatgcta cacgaagact ttggctatta ttcttatccc gaacactatc
     4201 gtttcggtga tattgttgtc ctagtctctc atgatgtaag taaaggtgtt ttattagaac
     4261 tgaaaggaaa aggctgtcgg caatttgaaa actttctatt ggctcaacat cggagctggt
     4321 atgatttctt tcaagattgc atggaacata aaggtatttt caaacgcttg gatttagcta
     4381 tcaacgataa aacagggatt ttgaatattc cagatttggc aaagaaatgt aaacaggaag
     4441 aatgcatttc cgtctttcga agctttaaaa attatcgcag tggtgaattg gttcaccgtg
     4501 atgaaaaacc atacatggga aacacacttt atattggctc attaaaaagt gaagtctatt
     4561 tctgtatata tgagaaagat tatgagcaat acgtgaaaaa tgatgttccc ttagaagacg
     4621 cagaagtaaa gaatcggttt gaaattcggt taaaaaatga ccgagccacc caagccatga
     4681 aagatttatt ggcacatcaa caggcggaaa aaacagcctt tgaaattatc aatcgctata
     4741 tccgctttgc agataaagac gacactaaac gtcggagtga ttggaaaact aatgaacgtt
     4801 gggaatggtt tatcggtaaa aatcgtggag cattacgcct aaccacacag ccagagcctt
     4861 attcttttga acgaacattg aactggttac atcatcaagt cgcccctaca ttaaaaatcg
     4921 caagcatttt agatgttctc aacggtacaa ccattatctc caccatgatt caggaagcca
     4981 aactgacaga aaaacatgag aaattaattg aacaacaaca tctagccatg gaggatttga
     5041 ttacatgagc cttttaacaa aagaattaaa gaagttaggg tttcagtgtg gcattgagtt
     5101 tcaagcgtat atacaaaata cagggaaata tacgtctctc attattgagg gaaaacggca
     5161 agctggtgat accatctata cctatgattt ttacaaggtg agattttata ataattatac
     5221 caatcgagta accgtctatg gagagcattt aacccctttt caattattaa ggcgtgtaaa
     5281 aagctatatc tattatcgag aaaaatatct taaggaaagg aggacaatca catgaatttt
     5341 gggcagaatt tgtacaactg gttcttggat aatgcacagt cattggtatt gttagcgatt
     5401 gttgtcattg gactgttttt aggttttaaa cgagaatttt caaaactcat tggcttcttg
     5461 attattgctc taatcgccgt cggacttgtg ttcaatgcag ctggcgtgaa agatgtgtta
     5521 ttaaacttat ttaaccgaat cattggtgct taacagatat ttttcatgat aaaataggaa
     5581 gtatatattt tgttatgaaa ggatttatga atatgtttac aaatgatcaa tttaaatctc
     5641 ttttaattaa aacattagaa agaaagagct cttcaaatta ttatgaagga ggtaacgaga
     5701 ttgtttctaa aatgaaaata tcagaggtaa ctttagatga gactgacgat tttacttatt
     5761 ataaaggata taaaaagggc tggaatacgc tttgcacata tcttaaaata agagttcctt
     5821 ttgatgattt agactttttt gaaagtcata aagatttgat tacccagaca gcaagctcca
     5881 tatatgataa acaaggtgat aatgtattag tggatactat tttagtacca cttccagaga
     5941 attacgaagt aattaacttt agtcagttga aaattagtga tgtggtttca caagctattg
     6001 aagacgctga gtcttttatg agtaatggtg aatatcaacg tgcctttgac agagttcaca
     6061 ctgcttttca tggatattta attgaaatac taaaaaaata tgaaattaca gttcctagag
     6121 acgaaaatct atctaaatta tatagtagga tacaacaatt aattgaaaaa gaaattcaac
     6181 ctactgaact tgctgatata gtgaagacaa cgataaggtc atcaaatgga atgattagct
     6241 ctcttaatga agcaagaaat aggcattcac ttgcacaccc taatacaaat attattggaa
     6301 agagagaagc aaaactaata ataggtattt caagtacagt tacagattat attagcggat
     6361 accttgataa gtagcgtcat taactgattg ctactttttt tatgactatt tttataaaaa
     6421 ggatgtgata agaattggaa atgaaagtct atgtggcaaa tttaggacgg tataatgaag
     6481 gtgaattagt cggagcatgg tttactccac caattgatga ggaagaaatg gcagaaagaa
     6541 tcggtttaaa tgaggactac gaagaatatg caatacatga ttttgagctt ccttttgatg
     6601 tggacgaata cacgccgatt agtgaaatca accgtctatg tgaagccatt caagaaatcg
     6661 aaggtacacc tatttataat gaactcaaag aaattcaagg catgtggttt agtagcttgg
     6721 aagaattact ggaaaacaaa gaggatattc attgttatag tgattgtgat tcgatggaag
     6781 atgtcgcccg ttattacgtg gaagaaacag gacagcttgg tgaagtccct tccaatttac
     6841 aaaattatat tgactaccaa tctttgggac gtgatatgga aatagagggt aattacctag
     6901 ttacctctca tggggtattt gaatattgtc agtaagttta acaatccgtg ttataattaa
     6961 attaataaat agcacggaga ggtataaatt atggtaaatg tatttgaaaa tcttaaacta
     7021 ttaaaactag acatgaaaga taaaggctgg gtgatagatt ccttttactt tcgttacaaa
     7081 cagcaaaatt atattgtact tgttaagcta tttgaaaaag aggaaaaagt tccagaatat
     7141 gctctgttaa aactagaatt tttaaaggaa aacgattttt cagatatgct agctgtctat
     7201 gctaattcgg taaaattata tacagacacg aaaacaataa gagaatattt tggtatcgaa
     7261 tacagtagta acttgggaga tgttttattt caatttagcc aaactcttgc tcgatttatt
     7321 ccgacagaag ttagtgagaa gaaaaatgaa gaccaaaaag aagccatgtg tttctcatta
     7381 agtcaaagtg attcagaaga tcccagaaaa aaatattgtt tttcagttag aagaaatcct
     7441 ttaaagggaa atggtgagct gggaaaacga agtcctttta atgacaataa aacgagattg
     7501 tatagaccca gcttatatga acgattaggg tcagatacca atttaagttt cagatattca
     7561 atgaatccag atgatgaaga aactgatgaa ggtattattg caaaatggac aaaaaataaa
     7621 attgaatagt taaagcattg aaagagcaat cacctatttt gattgttctt tttctatttt
     7681 tacggaaagg acgtgaagac atgaaaaaaa taagaagtta caccagtatt tggtcagtag
     7741 aaaaagtgat ttatgcgatt aatgatttac aactcccttt cccaattacc tttacgcaaa
     7801 tgacatggtt tatcgtttct ttgtttgtcg tcatactctt tggcagttta ccaccacttt
     7861 ctctgattga cggagcacta ctcaaatact tagggattcc cattgttctc actgggttca
     7921 tgtgccaaaa gacctttgac ggaaagaaac cctatggatt tctaaagtct atcattacct
     7981 atgctctacg ccctaaagta acctacgctg gcaaacaaat acgattaaaa aaagaaacaa
     8041 tgaatgaatc gattacgatt gtaaggagtg aacaactaga tggcatatcc gattaaatat
     8101 atggaagata atctcgtatt taatcatgat ggcgaagtat tcgcttatta tgaattaatt
     8161 ccctataatt acagtttttt aagttccgat gaaaaaattc aagttcatga taatttctgt
     8221 caattgattg cccaaaatcg tgatggcaag attcatgctc tgcaactagc aaccgaatca
     8281 agtattcgag ccacacagga acaatcgaaa aaagaaatca ctggacgatt aaaagagata
     8341 gcttacgaaa aaatagatga acagaccgaa gcgttaattt ctatgattgg tgacaatcaa
     8401 gtagactatc gcttttttat tggttttaaa ctctacctca atgaacagga agtatccgta
     8461 aaaaatgttg ggaaagaaat tcaaaaagca tttgcggaat tctttcatgg tgtgaatcac
     8521 aaattaatgg gcgattttgt ttctatgcct atggcggaaa tcgaacgctt ttctaagatg
     8581 gaacagttgt tagaaagtaa attatccaga cgctttcaga ttcgtaaatt ggagaaagat
     8641 gattttggtt atattattga acatctttac gggcaaacag gtgtcccttt tgatgactac
     8701 gactaccatt taccgaaacg atacgaagat aaaaaagtgt tggtaaagaa atacgatatt
     8761 ctcaaaccca ctcgctgttt aatcgaagaa aatcaacgct atttacggat tgaacaggaa
     8821 gataccacta cttatgtggc gtattttaca attaatagta ttgttggtga attggatttc
     8881 cctaatagtg aaatttttta ttaccagcaa cagcagttta ctttccccgt tgataccagt
     8941 atgaatgttg aaattgtcgc caataaaaaa gccttatcta ccgttcgcaa taaaaagaaa
     9001 gaactgaaag atttagatag ccacgcatgg gaaagcgaca acgaaaccag tactaatgtg
     9061 gtggacgctt tagaatctgt cgatgaatta gaaagtaatt tagaccaaac caaagaatct
     9121 atgtacaaac taagctatgt ggtacgggta tcagcaaaga atttggacga gctgaaacaa
     9181 cgttgtgatg aagtaaaaga tttttatgat gatctgtcta tcaaattagt acgacctttt
     9241 ggcgatatgc taggcttgca taatgaattt attccctcaa gcaagcgata tatcaatgac
     9301 tatattcaat atgtcacgtc ggattttttg gctggtcttg gtttcggtgc tacacaaatg
     9361 attggtgaaa attctggtat ttacgtcggt tacaatctcg atacaggcag aaatatttat
     9421 ctccaaccta gccttgcaag tcaaggaatt aaaggctctg taacaaatgc attagcgtcc
     9481 gcttttattg gttcattagg tggcggaaaa tcattctcta acaatatgct ggtctattat
     9541 tcagtactct ttggcggaca ggcaaccatt gttgacccaa aagctgaacg tggtgaatgg
     9601 aaagaaacct tacctgatat tgcccatgaa atcaatattg ttaatctcac gagtgagaac
     9661 gacaataaag ggttacttga cccttatgtg attttaaaac tcccgaaaga ttcagaaagc
     9721 cttgccattg atattctcac gttcttaaca ggtatttcca gccgagatag cgagaagttt
     9781 cctgtcctgc gtaaagctat tcggagtgta actcaaagca aacaacgagg gttgttattg
     9841 gtgattgatg aattgcgaaa agaagatacg cccatcagca atagtattgc tgaccatatc
     9901 gaaagctttg tcgattatga ttttgcccat ctgttattca gtgatggcag tgtcacccaa
     9961 tccattagct tggaaaaaca actcaatatt attcaagtgg cagatttggt cttacccgat
    10021 tccaatagct cttttgaaga atacactacc atggaattat taagtgtggc aatgctgatt
    10081 gtcattagta cctttgcttt agactttatc cactctgacc gttccatttt taaaattgtt
    10141 gaccttgatg aggcttggag ttttttacaa gtggcacaag gaaaagctct atctatgcga
    10201 ttggttcgag caggacgtgc catgaatgca ggggtctatt tcgttactca aaacgcggac
    10261 gatttactag atgaaaagat gaagaataat attggcttga aatttgcctt tcgttccact
    10321 gacctagtgg aaatcaaaaa gaccttagaa ttttttggtg tggacaagga agatgaaaac
    10381 aatcagaaac gcttacgtga attagaaaat ggacaatgtt tagtttcaga catctatggt
    10441 cgtgtagggg ttatgcagtt tcacccgatt tttgaagagt tgcttcatgc ctttgatacc
    10501 agaccgcctg tgcgaaatga ggtggaagaa tgacacaaaa acaaaagaaa tttcttcact
    10561 atagttggat aacgctcctt tgtgtgggcg ttattttact tttattaggc acgctgggca
    10621 atgcagtaca agcgacaggc ttagttgatg aaaccattga tacaagcaat gaatactcca
    10681 agtatggttt gaatcaatat cagcttgatt attatgtgga caatagctgg ggctggcttc
    10741 catggaattg gagtgatggc ataggacaat cggtgatgta tggattgtat gccattacta
    10801 attttatttg gacaatttct ctttatctat cgaacgccac aggttatttg gtgaaagaag
    10861 cctatgcctt agatttcatt agtcaaacat cccaagcaat tggcgaaaac atgcagacga
    10921 ttgcaggcat tacaccgaat ggcttttcca gttcagggtt ttatgtcggt ttcctccttc
    10981 tttttattct cattgttgga atctatgttg cctatacagg attggttaag cgagaaacga
    11041 cgaaagtgat acgagctgtt ttaaactttg tcgtggtgtt tattctatcg gcttccttta
    11101 ttgcgtatgc acccgattat attgcgaaaa tcaatgattt ttccaaagat gtcagtaccg
    11161 ccagtctgaa tattgggaca aaaattgttc ttcctaacac ggatagtcaa gggaaagaca
    11221 gtgtggacat gatacgtgac agcttattct ctgttcaagt aaagcaacca tggctattgc
    11281 ttcaatatgg ctccactgat gtagaagcgt taggggaaga acgtgtggaa agtttattgt
    11341 ctaccagtcc agatacaaac aatggtgaag atagagaaaa tgtagtgatt gaagaaattg
    11401 aagatcatga caatacctat ttaaccctac cgaagacgat ttccagatta ggaacagtct
    11461 tctttttgtt tgtattcaat attggtatct cgatttttgt tttccttcta acaggaatca
    11521 tgattttttc gcaagtgcta tttattattt tcgctatgtt tttgccaatc agtttcctat
    11581 taagtatgtt gccgagtttt gatggcatgg gcaggcatgc cattaccaag ttatttaatg
    11641 tgattatgac aagggctggt attaccttga ttattaccgt tgcctttagt atttccacta
    11701 tgctttattc gctgtcagca agttctccct ttttcatgat tatgttttta caaattgtga
    11761 cctttgcagg gatttatttt aaactgggcg atttaatgag cctattcact ttacaaagca
    11821 acgactctca aaacatgggc agacaagtgt ttcgcagacc tcgtcaaatg atgaatcgac
    11881 aaaccagaag atttcaacgc agtatgagta agatttttcg tggaaaagca gggtcaacga
    11941 agcaaaagac aatgacaaca aagcagaatc aaaccaataa agctaaccat actcgtcaaa
    12001 atgaacggac tacaccaaag aaagaaacag cactttccaa tgtgaaaaag caacaaggcg
    12061 gtatcgggca acgattaggt gaaaagacag cccatgtatt ggacacgaaa aatcgtctag
    12121 tcaataaaag aaagcaggca aaacaacaga tacaggcgac accaacccat gtcaaatatg
    12181 cccttcatca aggaaaagag aatgtcactc ataatgtaac agactttaaa caatctatgg
    12241 cagataccaa agctcaacgc aaacaacagc gtatgaacca gcaggagcaa aaacgaaaga
    12301 acattgctca aaagcgtttg gagctggata aagcaaaaga aaaaagacga aaagaaaacc
    12361 catcatctcc tgttcagaat aaccagcagc atgttcgcaa gcgtcagcaa accgtcacgc
    12421 ctgttttgtc acaaaacact aaacgtgaaa atcatcagcg accattgaaa aaagagcaaa
    12481 aacaaaagac accaccgaag caattcaatc agaggaagct aaataaaaga acggataagc
    12541 gaggacaaca aacatgaagc taaaaataac agtgatttgt agtgtggtct tctctctctt
    12601 cttttttctc ttattattcc tagtcatttt ctttgcagat gatacggaca gtggcgaaaa
    12661 caacaaggat tcttctattc cacaaggcgg tgtgaccgtt tcaccagaag tgttggcaca
    12721 ccgtccgttg attgaaaaat atggtaagga atatgggatt gaagactatg tttcttatat
    12781 cttagccatt atgcaagtgg aatcgggcgg tacagcagaa gatgttatgc aaagttccga
    12841 atctttgggt ttaccgccca acagtttgag taccgaagag tccatcaaac aaggggttaa
    12901 atatttcagt gaattattaa ccagtgcaga acaacaagga gtggatatag attctgttat
    12961 ccaatcttat aattatggcg gtggcttttt aaattatgtg aggagtcatg ggaaaaaata
    13021 tacctatgaa ttagccgaac agttttctaa agagaagtcg ggtggtcaaa aagcagacta
    13081 tcccaatcct atagccatac ctgtgaatgg tggttggcga tataactatg gaaatcagtt
    13141 ttatgttcag ttggtttcac aatacctaac cgacacttcc ccaacagaat ttgatgatga
    13201 aaccgtgcaa gtcattatgg acgaagcctt gaaatacgaa gggtttccct atgtctttgg
    13261 aggggcgtcg cctaccacct cttttgattg tagtggctta atacaatggg tgtatgacaa
    13321 agctggcatt tctctccctc gtgtcgccca agatcagtat gacgctacgc aagaaatctc
    13381 tatggaagaa gcccaagcag gcgaccttat cttctttcat tcaacctata atgcaggcac
    13441 atatgtgact catgtggcga tatatttaga gggaaatcgc ttttatcatg caggcgaccc
    13501 aatcggttat ggcgacttaa gcagtcgtta ttggcaagac catctgattg gggctagacg
    13561 tgttattcat aactaagaaa ggaatgaatc aaatgattat aaaaatcgag cgaaaagaaa
    13621 agaaggagaa accaaagaaa tctaaaaaac cgagcaagca aagaaaagtt ccgatgatta
    13681 aagttggcac gcataagaaa ttaacctttg ttttatgggt tttattgatt ggtagtgtcg
    13741 gatttggaat ctataaaaat tttactgcca ttgacactca tactgtccgt gaaacagaaa
    13801 tcattaaaca gcagattgtc gataccaacc aagtagaaag ttttgtgaaa tcctttgcga
    13861 aagactactt ttcgtggcaa caatcccaag aagccattga taaacgaaat gaaaaattga
    13921 cccattattt gacggaagaa ttgcaggtat taaatgaaga aatgattcgt aaggatattc
    13981 ctactagttc tagtgtgaac gatatacagg tgtggcaggt ttctcaagtg aatgagaaca
    14041 cctttgaggt actgttttct gttgaacaag ttattaccga agacaaagac aaggaaacta
    14101 tatcgtctag ctttcatgtg gttgtccata tagatgaatc agataatatg gttattatca
    14161 aaaatccgac aatgagtaag aaacctcaaa aatcggacta ccaacccaaa cagctagaaa
    14221 gtgaccatac agtagataca gaaacgatgg acgaaatcac tagcttttta gaaacgttct
    14281 tccagctcta tcctaccgcc acagaaaaag aattgactta ctatgtaagt aatcatgtgt
    14341 tacctatgat taacaaggaa tatgttttcg aggaattggt gaatcctatc tttaccagaa
    14401 aagataacca agtaatcgtg aatgtcgctg tgaaatattt agatcaagaa acaaaggcaa
    14461 cccaaatctc gcaatttgag ttgatattag aaaaacaaga taattggaag attgtaaaat
    14521 aacaaatatt ggtacatgat tacagatact ttgtaatcat gtactctttt tgataaaaaa
    14581 ttggagattc ctttacaaat atgctcttac gtgctattat ttaagtgact atttaaaagg
    14641 agttaataaa tatgcggcaa ggtattctta aataaactgt caatttgata gcgggaacaa
    14701 ataattagat gtcctttttt aggagggctt agttttttgt acccagttta agaatacctt
    14761 tatcatgtga ttctaaagta tccagagaat atctgtatgc tttgtatacc tatggttatg
    14821 cataaaaatc ccagtgataa aagtatttat cactgggatt tttatgccct tttgggtttt
    14881 tgaatggagg aaaatcacat gaaaattatt aatattggag ttttagctca tgttgatgc
//
LOCUS       DAJSBL010000062        14482 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.064, whole genome shotgun sequence.
ACCESSION   DAJSBL010000062 DAJSBL010000000
VERSION     DAJSBL010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14482
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.064"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(287..649)
                     /locus_tag="ONT85_002313"
     CDS             complement(287..649)
                     /locus_tag="ONT85_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2379266.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(723..1103)
                     /locus_tag="ONT85_002314"
     CDS             complement(723..1103)
                     /locus_tag="ONT85_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HCR2379267.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            1167..2360
                     /locus_tag="ONT85_002315"
     CDS             1167..2360
                     /locus_tag="ONT85_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HCR2379268.1"
                     /translation="MTGGLKMIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADM
                     DFLSPGGVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVL
                     TSLAAAVQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDME
                     KKIEENNVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHT
                     FTSMLTIDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEI
                     NTFGLIGTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLD
                     FTAYISDDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYA
                     LETID"
     gene            complement(2437..3021)
                     /locus_tag="ONT85_002316"
     CDS             complement(2437..3021)
                     /locus_tag="ONT85_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCR2379269.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            3222..4220
                     /locus_tag="ONT85_002317"
     CDS             3222..4220
                     /locus_tag="ONT85_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCR2379270.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            4332..4841
                     /locus_tag="ONT85_002318"
     CDS             4332..4841
                     /locus_tag="ONT85_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HCR2379271.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(4871..5530)
                     /locus_tag="ONT85_002319"
     CDS             complement(4871..5530)
                     /locus_tag="ONT85_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HCR2379272.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(5532..6296)
                     /locus_tag="ONT85_002320"
     CDS             complement(5532..6296)
                     /locus_tag="ONT85_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HCR2379273.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(6304..6981)
                     /locus_tag="ONT85_002321"
     CDS             complement(6304..6981)
                     /locus_tag="ONT85_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HCR2379274.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(7018..8403)
                     /locus_tag="ONT85_002322"
     CDS             complement(7018..8403)
                     /locus_tag="ONT85_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HCR2379275.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(8613..8697)
                     /gene="ffs"
                     /locus_tag="ONT85_002323"
     ncRNA           complement(8613..8697)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ONT85_002323"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(8720..9235)
                     /gene="tadA"
                     /locus_tag="ONT85_002324"
     CDS             complement(8720..9235)
                     /gene="tadA"
                     /locus_tag="ONT85_002324"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HCR2379276.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            9342..10199
                     /locus_tag="ONT85_002325"
     CDS             9342..10199
                     /locus_tag="ONT85_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR2379277.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYRYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(10280..10615)
                     /locus_tag="ONT85_002326"
     CDS             complement(10280..10615)
                     /locus_tag="ONT85_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2379278.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(10682..12052)
                     /locus_tag="ONT85_002327"
     CDS             complement(10682..12052)
                     /locus_tag="ONT85_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HCR2379279.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(12130..12666)
                     /locus_tag="ONT85_002328"
     CDS             complement(12130..12666)
                     /locus_tag="ONT85_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCR2379280.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
     gene            12975..14456
                     /locus_tag="ONT85_002329"
     CDS             12975..14456
                     /locus_tag="ONT85_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR2379281.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
BASE COUNT         4461 a         2883 c         2353 g         4785 t
ORIGIN      
        1 ttttttaaat tttttagaaa tgtgatataa catccacttc agaatcaacg ctgttaaatt
       61 tatcatgtat ttcttccttg gtcaactata aattaagcat tactttgtga aatgattttt
      121 cgatcaatta ttattattcg ttatacaacc gttttcaaca tattttgaaa ataaaatgca
      181 ctaaaaaact atctttagca gaacatttca ttgtattttt tggaccaaac aataaaaaaa
      241 gaagtttgaa aaaaattcaa acttctttgt tcgcttaatc tgtattttag aagaaaggca
      301 ctccctctac ttcgtaagtt ccaagaacag agcgcatttg acctggttct tcccaaatat
      361 atccttcgcc taattcttct ctttgattct tttgttcagt ttcaaagaca aacaacggaa
      421 cattttcctc tttaatatgt gtatttgtca attccatcaa actaatcttg ctaacatcga
      481 tatgaactat ttcttttaag aaattaagta tattcgctaa accagtcttt ccttcagaaa
      541 gttccgctaa cgcaaattcc aacttgtcat caattgaatg gaccaaaaat tttttagtac
      601 catcctccaa atgcaacata attgtaccag caactctttt gcttgccaaa atttccacct
      661 cgatcaactt gttctatggc ttacataaaa attctagcac attttcagaa aaaatacact
      721 ttttatttct tattttcttt ccgttctagt tcagataatg cttcttctat ccaaataggt
      781 aactgctccg caatagtggt aaatccgatc ttacgatttt tattcgtgaa ataccttttt
      841 ctgcgatatg gaacagctgg atttgttttt tctaacgttc ttaaagataa actgattttt
      901 ttggaatatt catcaatgtc gatgatctga actgttactg gctgtccgac ttttaacaat
      961 gaatggatat ttttcgtata accggattgt acttctgaaa catgaataag tccttgcgtc
     1021 tcgttatcaa gagaaacgaa tgcgccataa ggctgtatcc ctgtcacttt tccttcaatt
     1081 atttgtccaa ttttataagt catctacttc cctctcttcg catttatcat tatactgtaa
     1141 atagctcaaa aaaagaagcg acgaaaatga ctggaggtct taagatgatc gaatttgata
     1201 aacagatttc aagaaaagat acagaaagtg taaaatggga cgcaattgct gagacatatc
     1261 aaatggatga cttacttcct ttgtgggtag cagatatgga cttcttgtct cccggtgggg
     1321 tagctaaagc gtttagtgaa tacatcaaac atggaatctt tggttatgcc actctgtctg
     1381 ataaactata tcaggctatc attcattggg aacgccaaca ccatgctgtg gagttgacga
     1441 aagaaaatat cgtttttaca agtggtgtgt taaccagttt agctgcagca gtacaaactt
     1501 ttacaaaacc aggcgattct attttgattc atgacccagt ttatcctcca ttttcctcta
     1561 ttatagaaac aaatcaacgt cacatcgttc gaagctcttt gctagaaaaa aataatcatt
     1621 tcatcatgga cttgtctgac atggagaaaa aaatcgaaga aaacaatgta aaagcaatga
     1681 ttttatgcaa tcctcacaat cctggagggc gtgtttggag caaagaagaa ttgaaacagt
     1741 taagcgaatt gtgtttaaaa catcaagtga ttctgtttag tgacgagatc catcaagatt
     1801 taactttatt cgatcataca tttacttcca tgctaacgat tgatcctaat ttagatgatc
     1861 tactagttgc ttttacttcc gcaaccaaga ctttcaatct agctgcaatc aaaaattcaa
     1921 tggtatttat caaaaatcca gagatgaaag ctgattttga aaaacattta gtgatgaacc
     1981 aacagcacga aatcaacact tttggactga ttggaaccca agcggcttat gagacgggtg
     2041 gagaatggtt aagccaactg attccttatc tagaaaaaaa tgttcaaaca gttaatcagt
     2101 tttttgaaca gtatcttccg aaagtgagaa taatgaaccc tgaaggaact tacttaatgt
     2161 ggttagattt taccgcctat atttcagacg accaagaatt ggagaacact cttgttcgga
     2221 aaggaaaagt cgtattaaat cctggcatca cgtttggtcc ttccggtcat gggcatatgc
     2281 gcttgaatat tgcttgtcca gaagaaactt tactggaagg acttaatcga atcaagtatg
     2341 ctttagagac aatcgattaa atcatttatt caaaataaaa ggacgagacc ttactaggtt
     2401 tcgtcctttt tcataattac tccgtaccat accaaactat tctgaaattt caattgtttc
     2461 aatcactaca tcatgtacag gtcggtcttg tggaccacgt tgtacatttg cgatttcatc
     2521 tactgtgtcc atcccttcta gtacatgtcc aaagactgta tgtcggaaat ctagccaagg
     2581 agttccgcca cctttatacg cttcaatgat ttctgatggg aatccagcac cttctagttg
     2641 tccaagcata ttagctggca catttgtgtt atttacaata aagaattggc ttccgtttgt
     2701 gtttggacct gaatttgcca tagataaagc tccgcgaagg ttgaacagtt cacgactgaa
     2761 ttcatcttca aaactttcgc cataaatact ttcgccaccc attcctgttc cagttggatc
     2821 gccgccttgg atcatgaaat caggaataac gcggtgaaaa atcacgccat cataataacc
     2881 ttttttggaa agttcaatga aattttgaac cgtcttcggt gctagttcag ggaataattg
     2941 aacagtaatg tcaccacggt ttgttttgat aacagctttt ggtccttttt cactttttaa
     3001 atctagttgt gggaatgcca tattgtttcc tcctaatatt tgcatacttc ataataataa
     3061 taacataata cctaacctct cgtaaaaata ctagatttgt tttcttttat ttttcatcac
     3121 tagatacatt ctataactta ccgtttcgat ttcgaattca aacatttttg agaagcattc
     3181 tcaattaaat tctgctatag tgtgaaaagg gaggaatttt tatggaaatc tatgacatta
     3241 caatcatcgg tgcaggtcct gtagggatgt ttgccgcttt ttacgcagga atgcgccaag
     3301 ctaaaacaaa aatcattgac agtctacctc aacttggtgg tcaattagca actttgtatc
     3361 ccgaaaaata tatttacgat attcctgggt atccagcaat aaaagcaagt gaattaatcg
     3421 atcaattaga aaaacaatta actactttta accatacatt ccatttaaaa gaagaagttc
     3481 tctcgcttac tcgtgaagac gaagttatcg agattactac aaataaaggc atccattatt
     3541 ccaaagcagt cattttagct ttaggaaatg gttcattcca gccaagaaaa ttgaatttag
     3601 ataatgctga atcatttgaa aaccacggat tggattattt cgtgaatgat ttgatgaagt
     3661 atgctggaaa aaaagtcgct attgctggag gtggtgattc tgctatcgat tggacgttga
     3721 tgttagagcc tattgcaagc gaagtttact tgattcatcg tcgcccggaa tttcgtgcgc
     3781 acgaacatag tgtatcacgc ttgaaatctt catctgtcaa cttgcttact ccctatctca
     3841 tcgatggatt atcaggcaat aatggggaac tgacagatat ccggttaaaa aaagtgaaaa
     3901 gtgacgagac gattgatttg atgattgatt ctctgatcgt caattatgga ttctcttcta
     3961 atttagaaca tctttcttct tggggacttg atagtaccag aaatgccatt actgtaaaat
     4021 cagatatgtc tacttctatt ccaggcgttt atgcagctgg tgacatctgt acctatgaag
     4081 gaaaagtgaa actgattgct actgggctcg gtgaagcacc tactgcagtg aacaacgctt
     4141 tacactatat taatccaaag gaacgaactc agcctggtca cagcacaagc ttatacgata
     4201 aaaatcttcg tcctagttga ttttttatat ctctttgcat ttcaagttaa acccgaaagc
     4261 agtttcttgc atttttttca tgttccgtta acatgaaata gagaaagaaa aaataaaggg
     4321 ggagcaataa tatggtcgtc aaaactgaga ctttagaaga aaaggcaaga gaactattga
     4381 aggaacgtgg cgtaactgta gaagatatcg cacaacttgt catgttttta caaaaagact
     4441 atatttctga tttgactttg gcggattgta ctgaaagtgt caacagcgtg ctaacaaagc
     4501 gtgaagtaca taacgccatt atcactggaa tccaattaga tatattagca gaaaagaaaa
     4561 aattgatgag tccgttgcaa catattatcg aagatgacga aggcctttac ggtatcgatg
     4621 agatcctagc cttgtcaatc gtgaatgttt acggtacgat cggctttacg aattatggtt
     4681 atatcgacaa ggtgaaacct ggtattttaa aagaactaaa cagccacgat ggcgttcatg
     4741 tccatacctt cttagatgat atcgtcggag cgatcgccgc tgctgctgcc agtcgattag
     4801 cacacaaaga accagaaaaa agaagtcgcc ttactcaata attcaacaaa aaaagaaaga
     4861 aaacgtctat ttagacgagt tctttctttt ttgtttgttt caagctattt tttcctaaga
     4921 aaagaaaaac agcaaaaaat aattctaata atacaaaaaa taagataaag ctatgcatga
     4981 aaaattcttc tggaagtaca ttgatgattg gatcagtaac gggatcgaat aaccagtcat
     5041 cgttgttgaa aaacgtttca tgaaaaagga taaaaaagcg atcaaatcct atcatcatga
     5101 aaaaaccgaa aacaactggc agtaacatgc cgatttgaaa cggacgaatc aaccgccaaa
     5161 gccgatcatt cttttgcaaa tatttgataa acaaaatact cgggatcaaa gtgatgaaaa
     5221 acacagcata atccactaaa aacaaaattt tcacttcata aaagtgatgg gctccgctag
     5281 cagaaactgg gaaatctggc aagctgagga tggttttgaa gggattattg agataattca
     5341 ttaggtgatc aaaatttttc agtaacgtct cttgatctaa agaagtataa tccaaaattt
     5401 gcaaatgtcc gatatcaaat acgtatagtg gtcgaaagtt gatcgtcaca ccaactgcca
     5461 gactgatcag tgtcagaaat aggctgaaca atccagcata ttccatccac tgccatctcg
     5521 aatttttcat attagaagct ccattcatcc agtgattgtt tcacataggt agctgggata
     5581 ggcaacccag gtacatcttc ttcttttgta aaacctgata aaacgagcaa agtatctata
     5641 ttattgcgta tcccagcttg gatatctgtt tcatagttat ctccaaccat gattacttct
     5701 tctttttgca gccctaatac ttcaacggct ttatccataa tgatggcttc tggttttccg
     5761 atgtagatcg gcggcgtttg ggttgctgtc tctacaaaag agatgactga ccctgctcca
     5821 ggaagaaggc cgcgttcagt aggtatattc ttatctgggt tcgttccaat gaaagtagca
     5881 cctttttgaa tagcaagtgt tgctttaact actttttcat aggtcagata attatctaag
     5941 cctaccacta catagtcagg agcctcttct tcccatgtaa atccggcagc taaaatcaag
     6001 tcaatcaatc ctgcttctcc gataacatag acttttttcc cttttccatc tgctttcata
     6061 aaatcgattg ttgccaaagt agctgtgtag actgtctctg gagctacatg aatgtcaaac
     6121 tcatctgcta gacgattagc tactgtttct ggtgacttag tcgtgttatt ggtaacaaac
     6181 aagaaaggta attttctttt ttgcaattct tccacaaatc gtttaccggc cggtatgggt
     6241 tgtgtcccac gataaatcgt tccgtctaaa tcaattaaat atcctttata gtccattcat
     6301 ccactactct tccctttttc tgatcgtaaa tgctgttttt gttttttcta cttgtttagg
     6361 ttgttcttgt ttttttactt tttcagaggt ttttgctgac tctgtttttc gatttttaat
     6421 aaccggtttt ttgcgattag atgcgactgg tccttttttc tcatcgatat acgcacgatt
     6481 atggtttttt ttcttttttc ggcgctgagt tgtcttttct tttttaccgc cgattctctc
     6541 gatgacaaaa tacgcacaac caaaattgca atattcgtaa agatagtctt ccaaggaatc
     6601 gattcgctga tctggatgcg cccgtcgatt atccgcttgg aaaaatcctt ttaggcgtag
     6661 ttgttcataa ccccaatctc ctacgatata atcatatcgg gctagtacat cactgtaacg
     6721 ttctccaagc ttttctgcat taaatccttc acgataatcg gacacaagcc gataatgtct
     6781 ttcacctatc aaaaaattac tgtcatctaa cagtgtcact agctcgcctt tgactttttc
     6841 tgctttaggc tcctcaacga tttcttccaa agcagctgtc agttcttcgg tgactgttgt
     6901 ttcctgttca gctgatgatt ttttcttagc aatttctgtt ttatgctcaa cgttctgctg
     6961 ttcattttgt tttgcagaca tgcgttcacc acctatcttt tttattatac cttgttttta
     7021 tttgattggg tagtgagctt tcaagtaatc tcctaccaca cttcggataa attcagggaa
     7081 caaaaattca ttttttccag caatctcaat cgttggaaaa aatggaacaa acatcaaatg
     7141 atctacggta gtaaaagtat aatttttatt cagatcgatt ggcttgttct tccaaaagac
     7201 ttgatggtta atagaatcat atgtgattcc attatagacc atctgcccaa aaatcttacc
     7261 gcgaaatccc attcccttca taggatagtt acgtaaaaag ttacgatttt tttctatttc
     7321 taaaactaag cggatcagat catttccacc caatgtgaca tttaacaagt gcatcggatg
     7381 cggcaacgca gtgtgaagct gatcctgatt gacccgccct tttggaattt cagttaaaaa
     7441 cagaccactg tttaatattg ctgcttccgt ctttcctcgt tctttcaccg cctcaaaggc
     7501 tacttggata aacgaatgct cattgaagat atctagactc agagaaaatg gcaaatctgc
     7561 tacttctttt tcttttaata attcatgccc acgttctaga taactattta tttcttgttc
     7621 atcttctgta aaagctgtca ttgtttcggt agggatggct ttggctgaat gcttaaggat
     7681 ttttttctct tcatcgatgg ttaaatggac ttcaccgaca tactgcccgt actttccagc
     7741 ggctgctatt tggacatcat tgattacgtg accttctctg aataaatgat gggtgtgcga
     7801 tcctaaaata acatcaattg ctggtatttc ttttgcaatt tggaaatcat cctgaatccc
     7861 gagatgactc atcaaaacta aaacatccac ttgtgaacgt aaatcttcga ctaattctgg
     7921 caaaatatca taaggattcc gtatgtccca gccattcgga ttataagtaa gcggaaatgg
     7981 agcagtaaat gcaattagtc cgattttcgt ctggtgtttc gttgtaatga ttttatatgg
     8041 tttcgtccat gccggtggct ggagcgtatt tttatcgaat aagttatcca ggatgatatc
     8101 aaactcagca tgatcgtata aatgattcaa ctcttctttg gaatttccta cgccttcatt
     8161 gtttcctatc gtcgctgcat cataaccaac ttcattcatc agcaagatat tttcctgtcc
     8221 attcgttgct tctgacaatg gatgccaacg atccacaaaa tcacctagat ctactgcaat
     8281 cgttgtcgtt ttttctttct tttcgttttc tcgtttacgt tgatccaaaa accttctgat
     8341 ccttggccaa ttttctaaat gagaatgcag atcattcgta tgtaaaatca ctattttttc
     8401 catgtaccct ctcgcctctc tttatcttta tcataccata ggcagctttt tcttccattg
     8461 aaacaagctc tatttcagag tttttcctgc cttgaagcaa attgtataag tacaatattc
     8521 gataaatcag tctggctaaa aaatcaggaa cattcatttt tacagtacta taaaaataaa
     8581 aaaaacagac ccgaaagtct gttgtcgttt cattataatc agacacatgg cggaacctgc
     8641 ttagtgctgc ttccttccag acctgacacg cttcacaagc ccaccattgt cctagccttt
     8701 cggcaaagaa tagtatacct tatttttcat tcgttgacca gtcttttttt aatttctttt
     8761 ttcgctggcg gagctttcgg aaaaagtcag tcaaaatcat cccacattcg tcttctaaaa
     8821 tgccaccttc tacatatgct tggtggttaa accgctcgtc ctccaaaaga ttcatcaagg
     8881 taccggctgt tccgcctttt ggatcatacg cgccgaaata aacttctgct acacgagaca
     8941 aaatcatcgc gccgctgcac ataggacaag gctctaaggt aacaaaaagt gcagcatctt
     9001 ccaagcgcca gcttcccact ttttcacagg cttccttgat tgcaagcatt tctgcatgtg
     9061 tagtagcatc ttgcgtggtt tctctaaggt tatatcctct tccaatcact tttccatcca
     9121 atacaaccac tgctccaatc ggaacttcat gaagcatttc tgctttcttt gcttctgcca
     9181 atgcaaggcg catccatttt tctttttcta ctgcatcaag atttgtattt accaattacc
     9241 cttccctgcc tttgtttttt cttgttttac tttagcatat gataaaaagg ctgtaactaa
     9301 cttttttttc tatggtaaaa taaatacaag aggtgaacaa gatgaaaatg gataaattac
     9361 ttgctcttta cccacaagca cagaaaaaaa actctccagc atcagaagcg gatatccttt
     9421 cttttgcaat tgatgatgct tttatctgga tcaaacaaga ttcattatct caacaagaaa
     9481 ccagcctttt gaaagcgtta ttccctgtaa tcaacgatca aaaaaaacac ccatggtatc
     9541 gctatctttt taaagatgct tcttgtgttc aagaaaaaag ctttcgtttg atccagcttc
     9601 acgtcgaatc aaaaggagaa ttcttaaaaa aagaatggca agatactatt ttggaattat
     9661 ttcgctattc tgctgatttc tttttttata cagacacaga tgcgctctta gtcgaacaaa
     9721 taaaccgtga ttatcttgat gctactgaaa tcaacggtat ttttctatct ttagatgctg
     9781 attttgatac gacaacgtct gtattcgttg gttctttcca ttcacctggc accgacctcg
     9841 tccctttgtt tgctgaagaa agaaaaattt ttttaacaga gcaaaacaat ctttatactc
     9901 acagccgtac tttttctatg caagatgtgg ctttacatta ctatacaaaa gatactgtaa
     9961 aagatagtaa gttgatcaaa agttacatag aactgatcaa acaaaatgaa atggaagtga
    10021 tcattcaaga attatggaat gaattaggca atgtcagctc tgctgcaaaa acactgtttc
    10081 ttcatcgtaa tactctaaaa tatcgaatcg aaaaatttca agaacagtct ggtttcaatc
    10141 taaaaaaagc caacgattta ctgttttgct atcttttatt gcttcatgaa cataattaaa
    10201 gatagattaa aggggttgtg acaataattt tgtcacaacc ccttatttcg aatgaatagt
    10261 gttcaaaagc tagtttctct tacatcattt tttcaacaaa atgcattagc cggtctttta
    10321 catcttcggt tgaatctttg attgaatcac cataaacaga gattttcttt tttcctgatt
    10381 tgatttttgc taacattccc atcaaagaat ccaagtcact atctgaaaga ccatctacga
    10441 tttctagtaa tttttcattt ccatcaaatt tgtcatagac gaattttttt actttcgtcc
    10501 gagtagtcat atgatggatc ttttccatta cttttcccga tacaacgact gctgtcgcta
    10561 cactggcagc ggctgctacg cttaatccga ttgttacttt tgttgatgtt ttcataagta
    10621 tcagctcctt tttatctatc tccatcatag catagacaaa aaaataaacg aactaatcca
    10681 ctcagtgaaa tctcaatcct ttagggaaaa agttctttac tgttcctttt acttgtttcc
    10741 caaatccgac aggtatctta tctaatacta gcaaaaccca tccttgattc ccatcacgtt
    10801 gaaatgtttc tcctgctgtg taagattgcc attccttttg agtgatcggc aagcaaggta
    10861 tattctctat cttttttgta gcaagcgcta atgcatagct aggttcaaat cgattctttt
    10921 tgaaatctcc taaatgcaaa cctgtacgta cgacttttaa accatcgaga gaaggggcta
    10981 actcaggaac ttcccaaaga tggtcgttga acactaaaag tcttccagta gcttcataat
    11041 gaaaatcatt tgaaaactcc gtccagagtt tttcctgctc ttttgtcatt tgcactttac
    11101 ttttttttcg tgtcttcttt tctttatgca tctgattttg tccatgaaaa gtcagctttg
    11161 caacaaaatg tccttcaccc tgatctttat gaggccatat acgtattgtt ttttccaaac
    11221 cagctacgct tccccattca gaacgtcctg aactaacgga ttgagtcaaa ggaatctcct
    11281 caatcgttac tggataattt tctacaagcc agctaatgat ctcttcattt tcttctggtg
    11341 caaacgtaca agtcgaataa atcagttgtc ctttattttt tagcattttt attgctgaag
    11401 acaaaatctc ttgctgacgt ttttgacagt atagcggaga ttcttctgtc cactctttta
    11461 ttgcatttgg atcttttcgg aacattcctt ctcctgaaca aggagcatca accacgatcc
    11521 gatcaaaaaa gcccgaaaaa tgagggacaa gttctgcggg tgcatgattt gtcactattg
    11581 cgttcgaaac tccccatcgt tctatatttt ctgataaaat cttcgcacgt ttcggaaaga
    11641 tctcattcgt cactaataac cctttcccct tcatttgagc agctaattgg gttgatttac
    11701 caccgggagc cgcacacaaa tccaaaactt tttcaccggg ttttgctgca gcagctgttc
    11761 caacgatcat tgcacttggt tcttggctat attcataccc agcttgatgg aggaaagact
    11821 ttccattgac agtgccaaga aatccctcat tgctgtaggg agcgggttgt aattcttcgc
    11881 tatggtaagt ttggaccatg tccaatcctg ccggttttag tggattccac cgaaatcctt
    11941 ttttcacgct tccttgttca agcgcagaaa aaaaatcgga tgcttcttcg cctaaaagaa
    12001 gacgatattt tttgataaat tgctgtggta acgttgtcgc ttcctccttc attccaatgt
    12061 ttcgttagac attttacaat cttttttacg aaaaataaag gtggctgatc tactttttca
    12121 tgttgctgcc taaggtgact gttcctttaa gatattttcc aagtattcat ccgtcacctc
    12181 gtttttatcc caaacagcgt tcatcatttc aagaaaatct tgagaagaat catctgagcg
    12241 caaacttccc aatgcattaa gccataccaa ttcaagcgac ttttcttctt tgatcttcgt
    12301 gtgacctttg acatatgaga aaaatggccg cttaaatagc gaatccatgt ctaaatcaga
    12361 ttttgcaatg atttcacgaa tactttcatg gatagaaaaa aaatctttca ataagtagac
    12421 aatgcttaaa acatcatcgg aactgcattc agctaagtag ttttcaaaat aaacagtgat
    12481 ttcttctaat atcttctctt ttaacccgct gattgatttg aaatgtgtat aaatcgggta
    12541 tgtagaacaa ccgcagaact ctgctaactc tcttgcagta attgacttca ttccatattc
    12601 ttccacgtat accatacttt tgaacagcaa atattcttta ctataatttg ttactctccc
    12661 catcattctt acctccgtct cttttcatct acccataaat aaataacata aataattcta
    12721 aaaatattat tgataattat caaaacatat ttagttatat ccattaaaaa tcaatttttc
    12781 cgccttaaaa acaagaatat cttgacaaaa taccctagga aataaacaat agaataaaca
    12841 aggtaacaaa aaaattaaat aacgttatat aaaaaagaag gcgacactct gtgagaataa
    12901 cacagactga aatgactttc cactcacagt ttttgtctaa ctatatacaa aaacagaata
    12961 aaaaggaggt atttatgcta attgaagcaa gaaaaaaaga gttacttgtt ctacaaatgg
    13021 tcattcaaaa aaataattgt tctttacaag aattatccaa tgacttacat attccaaaac
    13081 ggacaatcaa agaactgatc cgaaaaataa atgtatcact caagcaattt ttattgattg
    13141 atgaatttat ttattctaat cataaaggag agatccaaac caaagaaacc gatcaaacta
    13201 aaaagttagc tgttttttct aagttaaaat tatgctacct aaaagaatcc aatcgtttta
    13261 actatttgat gttactaatc aattatccac aaactgctgt accaaaaaaa tatttacttg
    13321 aacaacttta tatatcgccc tcctatctcg aaaaactgac aaaacagctc aatcattcct
    13381 taaaaaaatt ccatttacgg atcctatctg taaaaaattg ctgtactctt gaaggaaatg
    13441 agttatctat ccgtctctat ctttactttt ttctttcaga tacatttgga ggtaacgatt
    13501 ggccattttc ctcacttgaa ttaaatgcac taaaaatgaa cagtacagat gagcaaatag
    13561 aaaaaatttc aaaacaagaa aaaaatatct acttgctgtt aactttattc atattacgaa
    13621 acaaacaaca ggcatatttt ccaaaaactt cttctgaaat cactgaattg atggacttat
    13681 tgcagcatac acatgattat tcagaatact ttacttcttt ttcagtgagt cgcccttgtg
    13741 aagagacaga aacagagaga gcctacttta attatttttt ctattgtttt tttccaaatc
    13801 aaatctcttt tcgacaaaaa caggagattg gttttttatt ttccacaagc aaaaacctta
    13861 tttgtcgaca agtcggtcaa ttgatgactt acttaactca acagtttcct ctcaaccatt
    13921 tagaaaataa aataccaatt tatttttatt ttcttgtgtt ggcagttgtt tttttcgaac
    13981 tgttgggtga atccagcagt agtttttcta cactttgctt tccagaagcc acgtccaatc
    14041 attctagttc ttttatacag gaaaagaaat taagagaaaa aatcgataca attttttcaa
    14101 aaaatgggga aagaaaccaa atcagtcaat accttagtca attgatccaa cacttactac
    14161 aaacagaaac gaaagagcag ttactcatcc atttgcagat aaccaaacag tttacaggaa
    14221 attatcatct tcaaagccat ttgcagcagt tatttcgcaa agatgctgtt aaaattaccg
    14281 catattcaga acatgctgat ttgatcatta cagatcagca tgagaaaatt tcagatgaaa
    14341 cggagacttt ctatctggat acaacgcaca atacagtgtt atttggcaag ttaataaaag
    14401 cggttcatga actgtatatg aaaaaaggtt atgagaacaa aacaaagagg atgtgacaaa
    14461 attcttgtca catcctctta tt
//
LOCUS       DAJSBL010000063        14107 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.065, whole genome shotgun sequence.
ACCESSION   DAJSBL010000063 DAJSBL010000000
VERSION     DAJSBL010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14107
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.065"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            66..767
                     /locus_tag="ONT85_002330"
     CDS             66..767
                     /locus_tag="ONT85_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HCR2379282.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            764..1885
                     /locus_tag="ONT85_002331"
     CDS             764..1885
                     /locus_tag="ONT85_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HCR2379283.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            1900..3132
                     /gene="pepT"
                     /locus_tag="ONT85_002332"
     CDS             1900..3132
                     /gene="pepT"
                     /locus_tag="ONT85_002332"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HCR2379284.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            3264..4172
                     /locus_tag="ONT85_002333"
     CDS             3264..4172
                     /locus_tag="ONT85_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HCR2379285.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            4207..4479
                     /locus_tag="ONT85_002334"
     CDS             4207..4479
                     /locus_tag="ONT85_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HCR2379286.1"
                     /translation="MDVLLHNLLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            4490..5536
                     /locus_tag="ONT85_002335"
     CDS             4490..5536
                     /locus_tag="ONT85_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HCR2379287.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            5787..8396
                     /gene="clpB"
                     /locus_tag="ONT85_002336"
     CDS             5787..8396
                     /gene="clpB"
                     /locus_tag="ONT85_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HCR2379288.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQPNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(8547..9233)
                     /locus_tag="ONT85_002337"
     CDS             complement(8547..9233)
                     /locus_tag="ONT85_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2379289.1"
                     /translation="MEGLSVIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGIT
                     FIVESLLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWD
                     KILHAVSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLC
                     DQFLGMNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFE
                     SYKLEKVKNT"
     gene            complement(9211..9705)
                     /locus_tag="ONT85_002338"
     CDS             complement(9211..9705)
                     /locus_tag="ONT85_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HCR2379290.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(9725..10945)
                     /locus_tag="ONT85_002339"
     CDS             complement(9725..10945)
                     /locus_tag="ONT85_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR2379291.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            11092..12927
                     /gene="lepA"
                     /locus_tag="ONT85_002340"
     CDS             11092..12927
                     /gene="lepA"
                     /locus_tag="ONT85_002340"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HCR2379292.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEDEPKK"
     gene            complement(13021..>14107)
                     /locus_tag="ONT85_002341"
     CDS             complement(13021..>14107)
                     /locus_tag="ONT85_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR2379293.1"
                     /translation="DINDDGSRKKVEKVGGDTRPEAEAALRKVLSDIDETGQYFLGTD
                     TRVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKLTDLSPALLQNFINA
                     EFKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPKYEARPTTKKDLKII
                     SLEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVDFSNGTITVEKQMVK
                     NDGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYGKLYIDSNAVCTKED
                     GELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKMKEISERLGHSRISI
                     TMDTYSHVTDKMRNETVDIMENLRKNS"
BASE COUNT         4555 a         2592 c         2919 g         4041 t
ORIGIN      
        1 ttttttattc ctagtctcct tattttattt ggaaaatgct ataattatct agaaggagtg
       61 aagctatgaa ccatttagat ttatctcagc gcttagccgc agtgggtgac tttgtccctg
      121 aatccgctcg tttagcagac attggatcag atcacgctta cttacccgtt gcattgatat
      181 taagaggaaa aatcgaatat gcagttgctg gtgaagtcgt tcaaggtcct tatgaatcag
      241 cacgtaagca agtcaaaaaa gatggattag aagaccgtat caccgtacgc cttgcaaatg
      301 gtctagatgc tatccagcca caagatggta ttacagctgt cacgattgcg ggtatgggag
      361 gttcactgat ccgagatatc ctagaagctg gcaagttgaa tcaacgctta agcggacaag
      421 aacgtttgat ccttcagcca aacatcggag agcgtacatt acgatcttgg ctgcaagaaa
      481 atcagtataa aatccaatca gaaaaaatac ttgaagaaaa taaaaaaata tatgaaatca
      541 ttgtagccga aaaagaacaa aaaccagtta cttacaatga aatggagctg atgttcgggc
      601 catttctgct tgaagagaaa agtgagatct tcaaatcaaa atggacaaga gagcttcaac
      661 aaagaaaagc tatccgtgaa cagctcaaac aagcagctaa tcaagaggca cgtcaaaaac
      721 aaatcgagca ggaaatccaa atgattgagg aggttttaaa gccatgacaa tcagcggacg
      781 cactttcatc caaaagtttg aaagctattg tcctcagtgg ctagctgaag aaggagaccc
      841 agtcggactg catatcggaa cattggataa gccaatccaa cgagtaatga tgacattaga
      901 cgttcgtcca gaagtggtcg aagaagcaat caagaaaaaa atcgatttgc tgattgctaa
      961 acatcctcca atctttcgtc ctgtaaaacg gctggttact gatcagccac aagaaaaaat
     1021 gtatgcagat ttgttgaaac atgatattgc cgtctatgca gcacacacga acatggatat
     1081 tatttgggat ggactgaatg actggttttg tgaacttctc ggtattgaag tggagagtta
     1141 tttagtaaag actcatgaga ttcattataa aaaactggcc gtttatgtac ctgtggatca
     1201 tgcacagaaa atgcgtgaag tattagctgc agctggtgct ggtacacaag gtgattatac
     1261 cggtacaagt tttacctcga ttggacacgg tcgatttact ccggaagctg gtgcacaacc
     1321 agcgattggg aaagtaggga agacagaaca agttcaagag gcaaaagttg aggttatttt
     1381 gccggaaact atcgaaaaac aagtgattca agccatgcgg tcagctcatc cttatgagga
     1441 acctgcttat gatcttttcg ctatagatga accagtcgaa atgtttgggt taggacgagt
     1501 aggtgaattg ccgcaagaaa tttctataga ggcatttgta gaacaagtaa aagaagcgtt
     1561 ccaactagat ggactgagaa tagtccagcc aaaaaatgca aaatcctctg tgaagcgcat
     1621 tgcgatttgc ggtggaagcg gtgaaaaatt ttatccgcaa gcaatcgccc aaagagcaga
     1681 tgtctatatc acgggagata tctattatca tacggctcat gatatgcaaa gcgctgggtt
     1741 gattgcaatt gatcccggtc attatattga atcgttatgc aagcaacgat ttatcgagaa
     1801 atttgaatct tggaaacaag aagaaaattg ggatattaac tttttcgttt ctgaaacaaa
     1861 tacaaaccca tttcaattta attaaaagga gagaatacta tgtacgaaaa tcttttgcca
     1921 cgttttttac gatatgtcaa aacagaaaca cgctccgatg caacaagcaa aacgactcca
     1981 tcgactcaga cacaagttgc ttttgcgcaa gaattgaaaa aagaattaga agagttaggc
     2041 ttagctgatg ttttctataa tgaagataac ggattcgtta tgggaacatt accaagcaat
     2101 gttgatcatg aagtgcgttc cattggattt atttcccaca tggatacagc agattttaat
     2161 gcagtaggag tcaatccaca aatcattgaa aactatgatg gcgagtctac gatcgtacta
     2221 gatgcggaag aacgctatac attgaataca aaagattttc caaatttgaa aaattatgaa
     2281 ggccacacat tgatcacaac agatggcagt acattactgg gagcagatga caaatcaggg
     2341 attgcggaaa tcatgactgc aatggagatc ttgatcaata atccttctat ccctcacggt
     2401 accatcaaag tggcttttgg tcctgatgaa gaaatcggtg tcggagcgga taaattcgat
     2461 gttgagcaat tcgatgtaga ttttgcttat acagtcgatg gtggtccttt aggtgagttg
     2521 cagtatgaaa cgtttagtgc tgctcaagca aatattacga tccaagggaa aaatgtacat
     2581 ccagggacgg caaaagatac aatgatcaat gctctacagt tagcaatcga ttttcataat
     2641 cagcttccag ccgatgaagt tccagaaaaa acggaaggat acgaaggttt cttccatttg
     2701 atggcattgg atgggaatcc tgaagaagct tctatgagtt atatcattcg cgatcatgat
     2761 cgtgaaaaat tcgaagcacg caaagcacaa atcacacata tccaagaaga gttgaatcag
     2821 cgatttgatc aagaacgaat caaagtagat atgtatgatc aatattacaa catgaaagaa
     2881 atcattgaaa aagatatgag catcgtggaa ttagcgaaac aagcaatgat cgagcttggt
     2941 atctctccag tgatcgaacc agttcgtgga ggaacagatg gttcaaaaat ctcttacatg
     3001 ggtattccga cgccaaatct atttgccgga ggagaaaata tgcacggacg attcgaattt
     3061 gtttctttac aagtaatgga aaaagcaaca gacgtgattg taaaaattgc agaattgaac
     3121 acaaagagtt aaaatcgaaa aagaacagct tatgacaagg cgttcgtccc aatgatggaa
     3181 tgtcatctgt ctaaggctaa agggcaggac aatggaattg gttgtcccgc cctctttttt
     3241 gaagagtaaa taggaggtaa agaatggaaa agctgctagt tttttataac aaaagttcag
     3301 ggaaagatga gggtgagaaa ttagcacgct ggtttcaaga ttatgtaaaa gaaaagcagc
     3361 cagaattata tgtagaacta gctgaaactg gcccatcgat tgatgatgcg gaattagtca
     3421 agaaagcaga agccatagaa gctgatactt tagtaattat aggtggagac ggcacgattc
     3481 atcatatcgt ccaagcgttc caaaaaacat tatctcaata ccatgtcgga ttacttccag
     3541 gtggaactgt caacaacctt gcacgtgttc ttgagattcc cctcaaaaaa gaagaagctg
     3601 ctgacgtgat cctggaaggc aatgtacgga aaatagacta tggtcaagtc aatgatgaag
     3661 tcattatctc tacactgaca atcggaatct tagcagatac agcagtttgg atcagtcaag
     3721 aagaaaaaca gcgttacggc tcgtggatct ttatcaagaa gttttttaag ttattgctga
     3781 agaagaaaaa atatcatttg gatttagaaa cagacgagga gcattggaaa ggaaaatccc
     3841 aactgttgac agtcacgatg tctaactccg tgggcggttt tacaaatttt gacgactcag
     3901 cgaccctaga tgatggaaaa tttcatgtaa cgatcgtacc ttccttagat attttccgat
     3961 ttatttttta tttgcctaga atcatgagag gcaaattcta tacgatcccc ggcattactt
     4021 atttcacagc caaacgcatg aaaatccaag cgcaggaaaa atctgtgggc actcgaacgg
     4081 atggagacac gacagatgat cttccagttg aattatctgt tgtatcaaaa ggattaacag
     4141 tttattcacc aaagaaagaa gaaaaagctt agaaatagta gttaacagaa agttggaaga
     4201 agagtaatgg atgtattgtt acacaatctt ttgtggtttt accttttcat tgtgaatttt
     4261 tacttatttt gtctgatggg gtatgataaa catcaggcta gaactagcgg ctggcgtgtt
     4321 cctgagaata atttgatatt tgtcagcctg ttaggcggtg gtttgggagg attgatctct
     4381 cgaaaagttt tccatcataa aactcggaaa aagaagttta ctataggatt cgctgcaggt
     4441 gtacttgttg atttgattct catttatttt ttccattaag gagcaggaga tgtcgtctaa
     4501 aggaacgaaa actaaaatca ttctgaatct gcttttgctg attgtgattt ttgccattat
     4561 ttattattta atcaatcaat catttgcaga tatctttgcc gaattgctgt ctaccagtgt
     4621 tgttgttttg gcagcggctg ttatattagg gacagtttac caaatcgctg aagggcgctc
     4681 aatcaaagaa attgctgaac ctttcgcgcc gaattttaca acgaaagatg ggttcttgac
     4741 ctctagctat attgcgtttt accgaatcgt ttcgtttggt acaggaacgc tgatctcgga
     4801 gatttatttt tataacaaaa aaggaatgaa gctatcccag ggagcagggg tgacagcact
     4861 ccatatgatt atgtacaaac tggcagtgat cacttatgca ttagctggct tggtcatcca
     4921 attctctctt ttttattcta atggtccatc aatgatctgg tttatcatcg ctggtatcct
     4981 tttgacgatc gtgattgttg cctttttatt ggcgatgtcc gtcagcttga atgttcaaat
     5041 attttttgtc agcttatcga ataagttatt taaaagtgag cgtttgcgga aatgggtgga
     5101 tacatgcaat cagcagatct actctttaag ggaaacggtc caaaccatta tccaagacag
     5161 gactgcgcta ttgcggattt atctatggaa tatgttaaaa cttgtatttt ggtatgtact
     5221 tccttatttg attctgattg aaaaccatcc taaaatagat tttctactga ccatttcgtt
     5281 tatcagcttt gcagtagtgc tttcaggggt tattcctacg cctgctggta ttggttcctt
     5341 tgaatttgtc tatttgttat tattcagacc agttgttgga acagttgacg cagtttcttc
     5401 gcttttactg tatcgtttca gttcctttat cttgccattt ctctatggat ttgtgtatgt
     5461 gattgctgaa cggcgaagag tgatcaaaga agaaataata gaagtaaaaa aagagaataa
     5521 agaaaatcaa gggtaggtct tttataagat ctaccctttt tatcaaatca aagaaaaacg
     5581 ttatggataa gataatttta cgtttgtttg gctctttttt tatatacata caatcaattt
     5641 tgttttttta agatttatat aagtcgagaa aaaaagtcaa aaaactatgt gtatattttg
     5701 accaaaattg accaaagcga tataatagtt tttgtaaaga tcaaagaaat aagtgatctt
     5761 acctttaatt aggagggtat acctgtatga atattgagaa aatgactacg acgcttcaag
     5821 aagcgattgc tgaagctcaa cagattgcgg tcactcgaaa acatcaagat atcgacattg
     5881 cccatgtatg gaaaatcttt ttacaaccta atcattttgg tcgaaatttc tatacggatg
     5941 ccggattgga tgtcgaatct tttgaacatg aaattgatcg tctactggat gagtatccag
     6001 ttgtttctgg tggaaacgtc caatatggac aaaacctcag ccagaatttc tttcgattat
     6061 tgaatgaagc agaccaaata cgtgaaagct tcggcgacga gtttctttca acggaagtcg
     6121 ttattttagc gttgatgaaa ttaaaaaatt atccgttaac tgtttatttg aataaaaacg
     6181 gattatctga aaaagaattg cgtaaaaaca tcgaagagat gagaggagga gaaagagtga
     6241 cctcacaaaa ccaagaagaa caatacaaag cattagaaaa atacggcgtt gatttagtac
     6301 agcaagtcaa aagcggaaaa atggatccaa tcatcggtcg tgatgaagaa atccgtgatg
     6361 tcatccgtat tctttcacga aaaacaaaaa ataatcctgt tctgatagga gaacccggtg
     6421 tagggaaaac ggcaatcgtt gaagggcttg ctcaacgtat cgtacgcaaa gatgtgcctg
     6481 aaaatttaaa agataaaacg attttttcat tagatatggg tgcattgatc gctggagcaa
     6541 aattccgtgg tgaatttgaa gaaagactaa aagcagtctt gaaagaagtc aaaaaaagtg
     6601 atggacgtat cctcttgttc atcgatgaaa tccacaatat cgtaggggca ggaaaaacag
     6661 aaggaagtat ggatgccgga aatctgttga aaccaatgct tgctcgcggt gaattacatt
     6721 tgatcggtgc cacaacttta gatgagtacc gtcaatatat ggaaaaagac aaagcgttgg
     6781 aacgtcgctt tcaaaaggta ttagtcaaag aaccaacagt ggaagatacg atcagtattc
     6841 tgcgtggatt aaaagaacgt tttgagatcc accatggtgt taacatccat gacaacgcat
     6901 tagtagcagc agctacttta tctgaccgtt atattactga tcggttctta ccggacaaag
     6961 caatcgattt gatcgatgaa gccagtgcaa cgattcgggt agaaatgaac tctatgccaa
     7021 cggaattaga tcaagttact agacgtttga tgcagttgga aatcgaagaa gctgcactga
     7081 aaaaagaatc agatgatgca agtaaaaaac gcttgaaaaa tttgcaagaa gaattagctg
     7141 aacttcgtga agaagcaaat gcgatgaaga tgcagtggga aacagaaaaa gaagaagtca
     7201 attctgtttc agcaaaacgt gctgaaatcg ataaagcaaa acacgaatta gaagatgcag
     7261 aaaataacta tgatttagaa cgcgctgctg tattgcgtca tggtacgata cctcaattag
     7321 aaaaagaatt gaaggaatta gaagctaaag caaaagacag cgagattaag atggttcaag
     7381 agtccgttac cgaaaatgag attgctcaag tcgttggcag attaacgggt atccccgtaa
     7441 cgaaacttgt agaaggtgaa cgagaaaaac taattaaatt gaatgaaaca cttcataaac
     7501 gagtgattgg tcaagatgaa gcagttgatg cggtcagtga cgcggtaatt cgttcacggg
     7561 ctggattgca agacccaaat cgtcctctag gttctttcct tttcctagga ccaactggtg
     7621 tcggaaaaac cgaattagcg aaagcgttag ctgagaactt gtttgactca gaagatcata
     7681 tggtacggat cgatatgagt gaatatatgg aaaaacatgc tgtgtctcgt ctagtcggag
     7741 ctcctccagg ctatgtaggt tacgaagaag gtggtcaatt aacggaagct gtcagacgaa
     7801 atccttatac aatcgtcttg ttggatgaaa tcgaaaaagc gcatcccgat gtcttcaata
     7861 tcttgttaca agtcctagat gacggacgct tgactgattc aaaagggcga gtagtagatt
     7921 tcaaaaatac ggttctgatc atgaccagca atatcggttc acagttactg ctggaaggcg
     7981 tgactgctga tggaacgatt ccagaagcag tggctgaaca agtcaacacg ctattacgtg
     8041 gaaacttcaa accagaattc ttgaaccgaa tcgacgacac gattttattt acaccattga
     8101 gcctagacaa cgtgaaagga atcgttgaca aaatggtggc gcaattggca caacgtctgg
     8161 aacatcaaga aatcctgctg acaatcagtg atgaagccaa aacgtggatt gctgaaaatg
     8221 cttatgaacc agcatatggt gcaagaccat tgaaacgatt tatcactaag gaagtagaaa
     8281 caccactagc taaagaaatc gttgcaggac atgttatgcc taaatcaaaa gtaacgatta
     8341 ctttacttga tggacaactt catttcaaaa cagaagagct agaagaaatc gtctaatctt
     8401 ccattgagac tataaaagaa aaaaggggtc tgacacaact agtgtcagac ctcctttttt
     8461 caaataaaca gtgttccaga agtagtttct tcgaaaaata agccgaaatt tcttgaagct
     8521 gcccgcttct gtcacaacct cttttattag gtatttttca ctttttctaa tttataactt
     8581 tcaaagtaac gctcatcttt tttactttga gaccatgcaa acaatcccat taaggcagca
     8641 cctatcgtat ttgtcagcaa gtcgcccatc gtatccatca atgctgctcg tccgacaaac
     8701 agtgtgccat cagaagcagc aaaacgttga agattcatgc ctaagaactg gtcgcataag
     8761 aattcccaga actcccaaaa cacgccgcaa agtccagcaa acgcaaagcc gaataagagg
     8821 aacaaccaag gactggtttt tgatatctca gcatctttca tcaagtaacc aatcaatcca
     8881 tagccaagag ctgtcaggac cattggactc accgcatgta agattttgtc ccaaaaagag
     8941 atgatactga tcaaatgcat ccctgtgcct aaaaagacag agatgaacaa gaaaaaccag
     9001 taaaacaaaa cgattgctgc tggtatttta agtttgaaaa ctttcaaaat gacttgtggc
     9061 atgaaaatca atgcaatccc taataaagat tccacgataa atgtgattcc ttgagaagtc
     9121 gaatattttt gtgtgataaa ttcataaaaa ttatagcaga caacgaatac accaaaagca
     9181 agtaaagcta tttgcacctt tttgatttct ttaatcactg aaagtccctc catctaaaaa
     9241 tctttttact tctgaaagca actcaatttc tgaagaaacc acttttccgt tcaatttgat
     9301 caaacctatt gtatataagt tcaggtagtg aaattgattt tcagctgttt cttgtagtgc
     9361 agcgattttt ttcgggtggg ttccgccttg ctgtcgagta tcagtatata agccgatcac
     9421 tgggatgcct ttcgcataag ccacaccgat ttccgatgct accccggcat cgatcgtcag
     9481 cccatctagt aaagcaatca ttaaatcact agccaacact ttttctgtgt cagcaagagc
     9541 aatcattcga ctgtctgcat aagcagtttt gtcattgatt gcttcatttt cttgaggaag
     9601 atagatcgaa agctcagaag agagctctct gatttcttta actaactgtt ggttatataa
     9661 caaatcactt ttagcaaaca ttggtgcggc aaaataaatt ttcatctaaa catcatcctt
     9721 tttatcaaaa ataatcgcta aagtaagctt ccatgtttgg aatgatacgc tctagtgact
     9781 tcagtgtttc ttcatctgtt tctaaccgtt cgattcttgc caaataagaa gcacggcgat
     9841 aattcatcat caagcaagtc aatgtttgga tatccagtcg aacggatttt cctataggtt
     9901 catttaagac acggacttgt ccctgttcat cccatgttaa agcgaagaca ccattattcc
     9961 aaggagcgac aggatcttca atgatgaaat gaaaagcatc agctgttcca acaaacggaa
    10021 accgttgaag gaattctttt acatcaacga tccgagccat aaaatatggt tcgatctgct
    10081 ctttgatctg actatcttca atcaaaaatg ctaaaggctc atttttataa atatctccat
    10141 gtacccagta gatcatggaa aaatgtgcgg aaataaagtt ccataagcca tttcgagctt
    10201 cttgatcaag atagaacatt tcttttacgt gaaatatttc ttcagctacc caatagaaca
    10261 gaacacctct tggttcatgg ttgctatcgt agtagacagc ggctgtgcgc tcatcttcat
    10321 tttcgaaacg ccaatactct tcccaatgaa aatcactgcg gaataaagca ccatggtttt
    10381 gttgggcaaa tttgtcataa acggaaaata cgtctggatg atcaactggt aatcgctcaa
    10441 ccataccact tactgggatt tgtttaggta gctgtgtatc tctgatttta aaggaaagtt
    10501 tatcagacat gatttcccat cctttacgcc gatagtaggg gatactgtaa ggatacaaat
    10561 aagagatcca ttgcttattc ttgcgcatat tatccaatgc aacgataatt agatcttgca
    10621 tcaatccatg gttagcatat tctggataag ttccaactcc agtcacacca cccattttat
    10681 aacgtgcacc gtgaatattg acttcacaag gatagatagc gatttgagag atcagtttat
    10741 tttcgtggaa ccatccgaac actttcgata cttccaaaat cggttttttg gaacggataa
    10801 aagctcgttt attctcaaac ccgctttcct cgatgtctgc ttcagtcact tggaacacat
    10861 aggaaagcaa ttcattgaat tgatcgatat gttcttgagt gaccggtttt aattcaagat
    10921 tttctctaaa atctttttga tccatttttt catctccatt tattagcttg ataacagtat
    10981 agcaaaattc tacaaaaaaa acttctatag ctgactaatt aaaaactctt taaattactg
    11041 cttttcgttg gtataatgga aagtgctgat tatatgaaaa gaggagacgc tatggatata
    11101 aagaaaatga aagaacgcca agagcagatt cgtaattttt cgatcatcgc tcatatcgac
    11161 catgggaaat ctacattggc cgatcgtatt ttagaaatga cgcacactgt gacgtctcgc
    11221 gaaatgcaag atcagctgtt agactcgatg gatttggaac gtgaacgtgg aatcacgatt
    11281 aaattaaatg ctgtagaact caattacaca gcgaaaaatg gagaaacata tatctttcac
    11341 ttgattgaca ccccagggca cgtcgacttc acctatgaag tctctcgaag ccttgctgcc
    11401 tgtgagggtg ccgttttagt ggtcgatgca gcacaaggaa tcgaagcaca aacattggcc
    11461 aatgtttatt tagcgttaga caatgatttg gagatcctgc cagtcatcaa taaaatcgac
    11521 ctacccgcag cagatcccga acgtgtccgt caagaaatcg aagacgtaat cggaatcgat
    11581 gctagtgaag ctgtattagc cagtgccaaa tcagggattg ggattgaaga tattctcgag
    11641 cagatcgtgg aatatgttcc tgcaccaagt ggagacttag atgcaccgct aaaagctttg
    11701 atctttgact ctatttatga tagttaccgc ggtgttgttc taaatgtccg gatcacggac
    11761 ggtatggtca aacccggaga caaaatcaaa ctgatgagta atggcaaaac atttgatgtt
    11821 acagaggtcg gggtcttctc gccaaaagca gttgctagag actttttgat ggtcggtgat
    11881 gtcggttata tcacagcaag tatcaaaact gtccaagata cacgagtagg ggatacagta
    11941 acgttagcag acaatcctgc cgctgaagct ttaccaggtt accgaaaaat gaatccgatg
    12001 gtttattgtg gtctttatcc aattgatact tctcgttaca acgatttgcg ggaagcttta
    12061 gaaaaactgc aattgaatga tgctgcttta caattcgaac cagaaacctc tcaagcatta
    12121 ggttttgggt tccgctgtgg attcctcgga cttcttcata tggatgtcgt ccaagaacgc
    12181 ttggaacggg aattcaatct agaattgatc acgactgcac catccgttat ttaccatgtt
    12241 aataaaacgg atggtacgac tgctacagta gataacccgg cagattttcc tgaacctgta
    12301 acgattcagg atgtagaaga accattcgtc aaagcacaga tcatggtccc aaatgatttt
    12361 gtcggtgcag taatggaact ttctcaacga aaacggggag aattcattac gatggattat
    12421 ttagatgatt accgagtaaa tgtcgtctat aatattccgc tttctgaaat tgtctttgac
    12481 tttttcgaca aattgaaatc aagcacgaaa ggctatgcct cattggatta tgaaatgtct
    12541 ggttatcaaa aaagcaagtt agtgaagatg gacatcttgc tgaatggaga aaaagtcgat
    12601 gcattaagct ttatcgttca ccgagacttt gcctatgaac gtggaaaagc aatcgttgaa
    12661 aaactgaaaa aactaatccc acgccaacaa tttgaggtgc caatccaagc agctattgga
    12721 caaaaaatcg ttgcccgttc agacatcaaa gccttgcgta aaaacgtctt ggctaaatgt
    12781 tatggtggag atgtttctcg taaacgtaaa ctcttagaga aacaaaaaga agggaagaag
    12841 cggatgaaac aaatcggctc cgtcgaagtt ccgcaagaag ccttcatggc cgttctgaaa
    12901 atggacgagg acgaaccgaa gaaatagcaa gggtttgttt gaaaacagtg taaaaaataa
    12961 accatgttta catggatttg ccaccggttt gccaccggtg gtttttttcg gtggcaaaaa
    13021 tcaagaattc tttctaagat tttccatgat atcgaccgtt tcatttctca tcttatctgt
    13081 tacatgcgag taagtatcca tcgtaattga aattctgctg tggcccaatc gttcagagat
    13141 ttctttcatt tttgcgccat tttcgagaag aagtgtagca tgagtatgtc tgagagaatg
    13201 gaagttaaaa gagagggaga gtgcattcga tatcctccta gtattccatt tcactacact
    13261 tggcgtaact agctcaccgt cttccttcgt acatactgca tttgaatcaa tgtagagctt
    13321 tccatacttc attcgatttt ctaattgttg tttcttatgt tttttcagaa ttgctagcaa
    13381 ggtttgtcca ataaaaatcg ttcgattgga actgcttgtc tttggcgtac catataccca
    13441 tgcgccgtca ttctttacca tttgtttctc tacagtaatt gttccatttg aaaaatcgac
    13501 atcatcccac gtcagaccac aaacttcgcc aacgcgcatt cccgtataaa atccaatatt
    13561 caaaggaata tagaaaggat gtccttcagg agtaatttct agcatatgat caaagtcctc
    13621 aagagaaatg atttttagat cttttttagt cgttggtcgt gcttcatatt ttggtatttt
    13681 tacatacagc ataggatttt gcttgattaa tccccaagga taaaccgcca tattcagcgc
    13741 attcttaagg acagagtgag taatagtcat tgttttcttc gagtaaccct ttttaaattc
    13801 agcattgatg aaattttgta aaagagcagg ggagagatcc gtaagttttt tctttcctaa
    13861 ataaccgttt atatgatttt tgatggtaaa tcggtagttt tcataggtat tgtattttag
    13921 atttagttta acgtattcct ccatccaaaa atcaaggtat tgttttactc gagtatccgt
    13981 acctaaaaag tattgtcctg tttcgtcaat atctgataaa acttttcgta aagcagcttc
    14041 ggcctctggt cgggtgtctc cgccaacttt ctccactttt tttcttgagc catcatcatt
    14101 gatatct
//
LOCUS       DAJSBL010000064        13852 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.066, whole genome shotgun sequence.
ACCESSION   DAJSBL010000064 DAJSBL010000000
VERSION     DAJSBL010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13852
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.066"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..902)
                     /locus_tag="ONT85_002342"
     CDS             complement(<1..902)
                     /locus_tag="ONT85_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HCR2379294.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPADIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGMDGLGETNFPMILRKQAEPL
                     HAVDFLADYFKEKTNTSVIALGPLTNIAFALKVNPNIGKHMDRFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFENLKQTIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIEGAICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFSE"
     regulatory      complement(926..970)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            1185..2153
                     /locus_tag="ONT85_002343"
     CDS             1185..2153
                     /locus_tag="ONT85_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR2379295.1"
                     /translation="MVIFYFILILIIIGISYHFLSYFYAKKLVRAGVQRGYDWYEDTG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGTPKTIKSYDGLKLAGQMFIHPNKQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLVPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPNASIILHGGSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMVMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMD"
     gene            2266..2544
                     /locus_tag="ONT85_002344"
     CDS             2266..2544
                     /locus_tag="ONT85_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCR2379296.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARRDGKKIIPLCPYAKKEFEKKPEYQDVLRQS"
     gene            2662..2898
                     /gene="secG"
                     /locus_tag="ONT85_002345"
     CDS             2662..2898
                     /gene="secG"
                     /locus_tag="ONT85_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HCR2379297.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            3001..3756
                     /locus_tag="ONT85_002346"
     CDS             3001..3756
                     /locus_tag="ONT85_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HCR2379298.1"
                     /translation="MRRVRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKAD
                     YTVYAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFA
                     VRALEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSL
                     AEQQLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLQW
                     YPESGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            3759..6116
                     /gene="rnr"
                     /locus_tag="ONT85_002347"
     CDS             3759..6116
                     /gene="rnr"
                     /locus_tag="ONT85_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HCR2379299.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILIEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSEEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDITGRRDLRNQLIVTIDGEDA
                     KDLDDAVTVKKLDNGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHDIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILETMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWAAELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKARNGKFGKRE
                     GKQAGKRSEKNGKKNKRYSDSKQTFGKKKGKKPFYKDVKKKKKKK"
     gene            6138..6602
                     /gene="smpB"
                     /locus_tag="ONT85_002348"
     CDS             6138..6602
                     /gene="smpB"
                     /locus_tag="ONT85_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HCR2379300.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIVRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            6904..7623
                     /gene="atpB"
                     /locus_tag="ONT85_002349"
     CDS             6904..7623
                     /gene="atpB"
                     /locus_tag="ONT85_002349"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HCR2379301.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMMTSLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            7676..7891
                     /gene="atpE"
                     /locus_tag="ONT85_002350"
     CDS             7676..7891
                     /gene="atpE"
                     /locus_tag="ONT85_002350"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HCR2379302.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVIALILVFAV"
     gene            7982..8506
                     /gene="atpF"
                     /locus_tag="ONT85_002351"
     CDS             7982..8506
                     /gene="atpF"
                     /locus_tag="ONT85_002351"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HCR2379303.1"
                     /translation="MLNQLAIAEVGNAMLGNIIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            8493..9035
                     /locus_tag="ONT85_002352"
     CDS             8493..9035
                     /locus_tag="ONT85_002352"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HCR2379304.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNQAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYEYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMDYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLEKMRNQLNR"
     gene            9058..10614
                     /locus_tag="ONT85_002353"
     CDS             9058..10614
                     /locus_tag="ONT85_002353"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HCR2379305.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGNNSST
                     EDKLKSIQNA"
     gene            10628..11530
                     /locus_tag="ONT85_002354"
     CDS             10628..11530
                     /locus_tag="ONT85_002354"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HCR2379306.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMYQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            11553..12959
                     /gene="atpD"
                     /locus_tag="ONT85_002355"
     CDS             11553..12959
                     /gene="atpD"
                     /locus_tag="ONT85_002355"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HCR2379307.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            12973..13395
                     /locus_tag="ONT85_002356"
     CDS             12973..13395
                     /locus_tag="ONT85_002356"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HCR2379308.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
BASE COUNT         4575 a         2474 c         3013 g         3790 t
ORIGIN      
        1 ctttcggaaa aaagacgagt atctactttt gtcataattt tactgttagc aggtttctgc
       61 caaaattcat aacgatcaac caatgtttgt ccacgactta ttccctgagt ttccacagcg
      121 gtgaagcttt tgaaaccttc acagatggcc ccctcgatga aataagcaac agctaacgga
      181 tcattgatga cacatcctaa aattctttct tgtttccagt ggaagtcaaa ataaaaacga
      241 gtgatcgctt ttaaatactc tccctcttca ggattcattt gacagcagta ttcgaggata
      301 gtcggtgtca agacgatttc tcttgtcaca tctaacccaa ccatttctat cgtttgtttg
      361 agattctcga atacataaga agctgcttca ggatcacacc agtaattgta ttcagcaact
      421 ggcgaacagt taccatggct tttgtaagtt ccacccatcg agacaaaacg atccatatgt
      481 ttcccaatat ttgggttgac tttcaaagca aaagcgatat tggtcaaagg acccaaagca
      541 atgactgatg tatttgtctt ttctttgaaa taatcagcca aaaaatcaac ggcatggaga
      601 ggttctgcct gttttctcaa gatcatcggg aaatttgttt cccctaaacc atccatacca
      661 tgagtgtctt gtgcactgat gaaaggttgc ttcaaaggtt tttccattcc ttgataaaca
      721 ggaatgtcta aacgatcaca gcgttccagg catttcagca cattctgcgt acctatatct
      781 gcagggacat tcccacaaac tacggtaata gcaactactt cgatatcagg ggatttgaca
      841 gctaatgata aagctaaagt gtcgtcgatc cctggatcgc aatcaataat tacttttcgc
      901 attatttcca atctcctttc tttagagata cttagttttt tattctggga agtttccgaa
      961 ccagtccaag acgagcgtct tgattataat gcacgaaatt agtatactgg cttttattgc
     1021 gaatgtaaat gctctcttac aagtaaatct attgaggaga actttaagta aatgatacaa
     1081 tgaaaaagaa atggaattta cgtgtgccga aaataaagaa tcctatggat cggtacattg
     1141 gataatagat agattatagt ttggcagaaa gcggcgggtg tacaatggtt attttttatt
     1201 tcattcttat tctgataatt atagggatca gttaccattt tctttcttat ttttatgcaa
     1261 agaaattagt aagagcggga gtacagagag gatatgactg gtatgaagac actggtcatc
     1321 gtttgatgga ccctgctgca gtcacgtcag tagaaaaaaa acgacaaaaa ttagaagaag
     1381 agtgtgaaag cttttgggag cagggaacac ccaaaacaat caaaagttat gacgggttga
     1441 aactagctgg tcagatgttc atccatccaa ataagcagaa taaatgggtc atttgcgtac
     1501 atgattaccg aagcacaggg aaaagagaca tgtctcatat aggtaaaaga tatgcagaga
     1561 aaggattcaa cgttcttgta cctgatttac gtgcgcacgg agaaagtgaa ggagaaatca
     1621 tcggaatggg atggctagat cggctagatt taattgcctg gatccaactc atcttagatg
     1681 aacagccgaa tgcgtccatt attttacacg gcggatcaat gggagcttct acgatcatga
     1741 tggcaagtgg tgaaaaactt ccatcagcgg tcaaaggatt tattttagac agcggatatg
     1801 tttctgttta tgcagaattc cgatatatgt taagtaaaat cactgtcttt cctaaaaaaa
     1861 tggtcatgag atatgcgaat cattatgccc agaaatacgc cggttactcc ttaaagcaag
     1921 catcagctac gagacagctt ggcagcaacc atctgccttt attagtcatc catggtgaaa
     1981 gagatcattt tgtccctaca gaagcagcgt acacgattca aaatgctacg gcaggggata
     2041 aagcactgct gcttgttcca gaagcagaac acttggaagc atcgatgaaa gatccgaaaa
     2101 cctattggac agtgattttt tcatttattg aacaaagaat aacgatggat taattttctc
     2161 ttcttttttg taagtaattc gaggataaat aattgtttat agatctggag actgctaaaa
     2221 taaggtagga ataaaaaaat atcagttatt tgaggagtgc ggcatatgga aatcaaagaa
     2281 gaaaaaaatc gtcttgcttt attcaatgat gagcaacaag agattggaga aatgacatgg
     2341 tcagatgcgg gcccagacat catgatcatt gaccatacat ttgtagatcc agcatatcgt
     2401 ggtcaaaagt tagctgaaaa gctagtgtat actggagtag aattagcaag acgagacggg
     2461 aaaaagatca ttcctttatg cccttatgca aaaaaagagt ttgaaaagaa accagaatat
     2521 caagacgtct tacgtcagtc ataagaataa cagctgaaag ttttgaactt tcagctgtta
     2581 ttccatttat atagagttgt cacggacttt tatctatgat aaagttaaag agacgatatc
     2641 ttttgggaag gaggctgcga tatgtataat ttaattttgg gaatcgtgat cgttttatcg
     2701 attgttatgg tcattgcgat tatgatgcag ccaagcaagc aaaatagtgc agcgagtgca
     2761 tttaccggcg gtgcggatca actattcggc aaacaaaaag ctcgcggctt tgaagctgtc
     2821 atgcagcgat caactgcagt tatgggagct atctggatga tcttattatt tgtcctagca
     2881 tatttatctt cgaagtagtt gaataagcct ctcgcaaaac ggcgggcggc tttttttatt
     2941 gcaaagagaa cgcttaagaa gaagttatga aagaaactat ggtaaaatga ggcgaaagag
     3001 gtgagacgag tgagacaatt accaaaaccc ctttatgcaa aacatggaaa gcgagcggtt
     3061 ttgctgcttc acgcttactc aggaagtcca aacgatgttc gaatgctcgc acgatttttg
     3121 gaaaaagcag attatacagt ttatgcgccg ctttttaaag gacacggaac aatggagcca
     3181 tatgatatct tgcaagaaaa agcagaaagc tggtgggcag acactaaaaa agccatccat
     3241 tttttacagt ctgagcagtt ttctgatatt gccgtattag gattatcaat gggcggcatc
     3301 tttgctgtac gagcactaga agaagaatca gtgattggcg gcggtttttt ctgttcgcct
     3361 ttgtctcctg taaaaacaaa cgtgcctgaa aactttgaaa aatacgtacg tcaagtgctg
     3421 aagacagcag gtaaatcaga gaaagaaatc aatgaaaaag cagttgctta ccgctcgtta
     3481 gctgaacaac aattaatgga tatacaagat caggcagcaa tcgtagaaag taggttgtca
     3541 gatatccaac agccgatatt tttagctcaa gctgggaaag atgaaatgat cgaccctaat
     3601 ggcgtatttg aaactgcacg caagttgagc cgtcagcgag tgacacttca atggtatcct
     3661 gaaagtggtc atgtgattac agttggtaca gcaagacgcg aattagaaaa agacgtcttg
     3721 gaatttttag aaaaattacc ttggaatgag gaataaatat gacaaaagaa acgataaaag
     3781 agaaaattct ctttttcatg gaaagcagtc gcaaacgcag tttttctatg gaagaaatcg
     3841 ctgaaggttt agggttccaa aaaagcgatg attttaaatt actagtacaa acgattgctc
     3901 aaatggaaag agaacagagt atagttttca ataaaaaagg aaaagtgaaa cttcctttaa
     3961 aaccaatctt gatcgaagga acatttcgag caaatgaacg aggatttgga tttgtaacga
     4021 ttgatcccga agaagatgac atctatatcc caaaagaagc gactggtttt gcaatggatg
     4081 gagacaccgt agcaatcgat atcatgaagc gagcaaatac agatatggat cgcggagcag
     4141 aggggaaagt agtcgagatt cgagaccggg cgactactca gttagcaggg gaattcgttg
     4201 cttattcaga agaagaaata caagaaacag atctttacgg cgttgttatc ccgaaagata
     4261 gaaagctcaa ccaatttaaa gtatatgcag cagcagaggg agttcgtcca gttgacggaa
     4321 gtattgtgct gatcgaactg acccattacc ctgaaaaagg gtatgcgact agtttagaag
     4381 ggataatcaa gcaagtgatt ggtcataaga atgaccctgg catggatatc ctctcaattg
     4441 tcgtggctaa tggtatccct acgaaatttc ctgatgatgt attgtctgaa gcagatgaag
     4501 ttccagatca aatcagcgaa aaggatataa ctggacgtag agatttaaga aatcagctca
     4561 ttgttacgat tgatggagaa gatgcaaaag atctagatga tgcagttacg gttaaaaaat
     4621 tagataacgg taattatttt cttggagtac acattgcaga tgtttcttac tatgtgacac
     4681 aaggcagtca attggacaga gaagcatatg aaagagggac aagtgtatat ttgactgatc
     4741 gagtgattcc gatgatcccg caacgtttat cgaatggtat ctgttcactg aatccccatg
     4801 ttccaaggct gaccatgagt tgcgaaatgg agattgatcc aaatggacaa gtagtctctc
     4861 atgatatttt cccaagtgtg atccaaacaa cagaacggat gacctacaca gctgtcaatc
     4921 aaatcttaga agatcaagac gaagaaacga tgcaacgcta tgctcagctc gtacctatgt
     4981 tccaagaaat gggtgaattg catcgaatcc ttgaaacgat gcgaatcaaa cgtggcgcga
     5041 tttcttttga ggatcgcgaa gcgaaaatcc ttgttgatgc tgaaggacac ccaaatgaca
     5101 tccagctaag aagcagagga attggtgaac gcttgatcga atctttcatg cttgcggcca
     5161 atgaaacggt agctgaacat tataataaat taaatctgcc gtttatttac cgaatccatg
     5221 aacagccaaa agaagaaaaa atgcagcgct tctttgattt tgcttcagct ttgggcattc
     5281 tggtccgagg aacgaaagga acgatcacac caaaagattt acagcatgtg atcgaaggag
     5341 tagaagacaa accagaagct gcagttatca atactatgtt gctaagaagc atgcaacaag
     5401 cacgctattc agaagacaat tttgggcatt atggcttagc ggcagattac tacacacact
     5461 ttacatctcc tattcgacgc tatcccgatc taatcgtcca tcgtctgatc cgttcttaca
     5521 gtgaagataa aagtgaaagc aatcaaagtt cgtgggctgc ggaacttcct gaaattgcag
     5581 atcatagttc taagatggaa cgtcgtgcag tagaaacaga acgtgaagta gattccatga
     5641 agaaagcaga attcatgatg gacaaagtgg gagaagaatt cgatgggatc atcagctctg
     5701 ttgtgaaatt cggcctgttt atcgaactgc cgaatacagt agaaggattg atccatatca
     5761 atgaattgaa acaggactac ttccattttg ttgaaaatca tttagctctt gtaggtgaaa
     5821 gaactggtct gacttttaaa atcggacaaa aagtgcgcgt aaaagtagtc aaagctgatc
     5881 cagatgaacg agcagtggat tttgaactcg tttccgcaga agaggtcact gcgttggaac
     5941 gtccaaaagc acgcaatgga aaatttggaa aacgagaagg aaaacaagca ggcaaacgct
     6001 cagaaaaaaa tggcaagaag aacaaacgat atagtgatag taagcaaacg ttcggtaaga
     6061 aaaaagggaa aaagccattt tacaaagatg tcaaaaagaa gaaaaagaaa aaataaaatg
     6121 agttggagga aacagctatg ccaaaaggcg agggaaaact aattgcacaa aacaagaaag
     6181 ctcgccatga ttattcgatc atcgacacga tggaagcagg gatggtcttg caaggaacgg
     6241 agatcaagtc gatacgaaac agccggatca atctgaaaga tggatttatt cgcgtccgca
     6301 acggggaagc tttcttgcat aatgttcata tcagtcctta tgaacaagga aatattttta
     6361 atcatgatcc gttgcgcacg agaaagttat tattacacaa gaaacaaatc gttcgtcttg
     6421 aaaacgaatt gaaaaacact ggaatcactg ttgttccttt aaaagtctat attcgtaacg
     6481 gctatgccaa ggtattgatt ggtctggcaa aagggaaaaa atcgtatgat aaacgggaag
     6541 atttgaaacg aaaagatatc gatcgacaaa ttgatcgaac attaaaaaat ttctctagat
     6601 aatttcatta tcctttttcg catattcggt gcttttgctt ttacttaatg ataaaatttg
     6661 ttagcattta atctgataaa acatgattat cacaggtttt ttcttagatc aggagcaccg
     6721 tgcatgggga aaaattcgct tcgtttttga ctgaaataaa aaagtgtcta attttcatga
     6781 aaatgttttg aattttctgt ttcatggtgg tatgataccg tgtggttaga aaccgattgg
     6841 aaaaaaccag atacgcacat gaattgattt tatgtgaatt tcggggaaaa gaggtgaaat
     6901 ggattggatg aacgctcgct aacgtttcac attggaccaa tttggtttga tggtacagtt
     6961 tgtataatgg tgttactgac ctgtttgatt gttttcttct tagcttactt ttttacaaga
     7021 aatcttaagt tgaaaccaac aggcaaacaa aatgcgatag aatgggtcat tgattttagt
     7081 cgaggaatcg taacagataa tctgcctaga aaagaattga gcaatttcca tttgttggca
     7141 tttacattgt ttttatttgt ttttgttgca aacaacatag gtttagtaac aaaaatcgtc
     7201 ttacctaacg aaacaacact ttggaaaagc ccgacagcag acccgtttgt tactttaact
     7261 ttggcattta taatgatctt acttactcac ttattcgggg tgaaaaagtt aggcttcaaa
     7321 ggatatttcg taaattcgtt cttgaaaccc tatagtttta tgtttccgat gaaactgatt
     7381 gaagaattta caaatttatt aacacttgct ttacgtcttt atgggaatat ttatgcgggg
     7441 gaagttttgc taaccttgat tgcaaaaatg atgacaagct taggctggtt ctcattacca
     7501 ttagcaattc cattagagat ggtctggatc gcgttctcct tgtttattgg gagcatccaa
     7561 gcatttgtct ttgtcacttt gtcaatggtt tatatgagcc ataagattga agtagaagaa
     7621 taatactcgg ttaaaaaaca aactactatt tatctatttt aggaggaaaa caattatgaa
     7681 ttatatcgca gcagcaatcg caatcatggg agcagcaatc ggagccggtt atggtaacgg
     7741 tcaagttatc tctaaaacaa tcgaatcaat ggctcgccaa cctgaaatgt ctggtcaatt
     7801 ccgtacaaca atgtttatcg gggtagctct agttgaagct gttcctatct taggggtagt
     7861 tatcgccttg attctagttt ttgccgttta attgaatcag tattaaggca gaaacaacat
     7921 cgtttgttac tgctttttta catggaaact agaaatactc caaagaaagg aaggctcgcc
     7981 tatgctgaat caattggcaa ttgcagaagt tggcaatgcg atgttaggta atatcatcgt
     8041 tgtcagcggc tcatttttga tactactggc tctcttgaag cactttgctt ggggaccaat
     8101 cagcgatatt ttgaaaaaac gtgaagacaa gatcgccaat gatttagatt ctgcagaaca
     8161 atctcgcatc aactcagcga aaatggaaca agaacgcgaa caacaattgt tagcctctcg
     8221 ttctgatgca gctgatatca tcaaaaatgc gaaagaaagt ggagaattaa gccgccaaaa
     8281 tattttgaag gatgctcaag aagaagcagc tcgtctaaaa agcaaagccc aagctgatat
     8341 cactgtagaa cgtgattcag cgctgaactc tgtaaaagac gacgttgcag aactctctct
     8401 tcaaatcgcg gaaaaaatct tgaacaaaga attatctcca gaaatgcatg aatcattaat
     8461 caatcaatac attgaaggtc taggttcttc aaatgaaact agataaatat acagtaggaa
     8521 gacgttacgg gaaagcttta tttgaactgg cgatcgattc aaatcaagca gaagaaatct
     8581 atcaagatct cgtaagttta cgtcaaatct atgaacaagt acctggatta gggaatatgt
     8641 taagtgatgt tcgtctagaa cctcatgaaa aacgagaaat catggacaag cttgtcagcg
     8701 gttatgatgg gatcgttaaa aactttttag aagttgttta tgagtataac cgaatggatg
     8761 accttttgtt catgatcgaa gaatatgaac gtcgatatga tgaacatcaa gggttactat
     8821 tgggaagtgt cacaacagca gtcccacttt cagacgaaca acgaatacaa ttagaaaaga
     8881 atgttgcaaa aacaatggac tatcaatcag ttgaattaaa acaaatcgtt gattcttcta
     8941 ttattggtgg cgcgattgtt gaagccaacc atcgagtaat cgacggaagc attcgcacgc
     9001 aattagaaaa aatgcgtaat caattaaaca gatagtacaa agaggtaggt gaatcgaatg
     9061 gccatcaaag cagaagaaat tagtgccttg attaaagaac aaattgaaaa ttatcaaaac
     9121 gtgctttcag tcgaagaaat cggtaccgta acttatgtag gtgacgggat cgctcgtgca
     9181 catggtttag aaaacgcgat gagtggtgag ttgcttgaat tttcaaacgg ctcatacgga
     9241 atggcgcaaa acttagagtc aaatgatgta ggtatcatta tccttggcga tttcgagtcc
     9301 attcgtgaag gagataaagt aaaacgaaca ggtaagatca tggaagttcc agttggagag
     9361 gccttgatcg gtcgggtagt caatccgcta ggtcaaccaa tcgacggact aggtgaaatc
     9421 gttactgaca aagctcgtcc agtagaagcg atggcaccag gtgttatgca acgtaaatct
     9481 gttaacgaac caatgcaaac agggctaaaa gcgatcgacg cccttgtgcc aatcggacgc
     9541 ggacaacgtg aattagtcat cggtgaccgt aaaacaggga aaacttctat tgcgatcgat
     9601 acgatcatca accaaaaagg tcaagatatg atctgtatct atgtagcaat cggacaaaaa
     9661 gattctacag ttcgtacaca agttgaaaca ttgaaaaaat atggcgcaat ggattacaca
     9721 atcgttgtga atgccggtgc gtctcaacca gcaccattgc tttatatcgc accatatgct
     9781 ggtactgcaa tgggtgaaga attcatgtac aacggtaaac atgtattgat catttttgat
     9841 gatctttcaa aacaagctgt tgcttatcgt gaactttcct tgctgcttcg ccgtccacca
     9901 ggtcgtgaag cttatccagg ggatgttttc tacttacact cacgcttatt ggaacgtgca
     9961 gcaaaattaa gtgatgattt aggtggcggt tcaatgactg ccttaccatt tgttgaaaca
    10021 caagctggag atatctctgc ttatatccca acaaacgtta tttcgatcac tgatggacaa
    10081 atcttcttag aaagcgactt gttctacgca ggtactcgtc cagcggttga tgccggacta
    10141 tctgtatctc gtgtaggggg ttctgcgcaa atcaaagcaa tgaaaaaagt tgctgggact
    10201 ttacgtctag acttagcaag ttaccgtgaa ttagaagcat ttacacaatt cggttctgac
    10261 ttggatgcag caacacaagc aaaactaaat cgtggacgcc gtacagtaga aatcttgaaa
    10321 caaaaattgc atgcaccgct agctgttgaa aaacaagtag tgattttgta tgctttgaca
    10381 catggattct tagatagtat ccctgtagac agtattttag acttcgaaca tgaattattc
    10441 gaatatctgg ataccaatca ttcagatatc tttgaaacaa tccgtacaac aaaagatctt
    10501 ccagaagaag aagcgttgaa cagtgcgatt caggaatata aagacatgtt cttagctaca
    10561 aaaggaaata attcctctac agaagataaa ttgaaatcca ttcaaaatgc atagtgaggt
    10621 gagatgaatg ggtgcttcat taaatgaaat caagacacgt atcgcttcaa cgaaaaaaac
    10681 gagccaaatc acgcgggcaa tgcagatggt gtctgcttct aagttgacaa aatctgaagc
    10741 ttcttcacag aagtttcagg tttatgcaac aaaagtaaga gaaatcgtta cgcatttgac
    10801 tgctactcag ttaaatgata ttgcctcaga aaatcctaga ggggatatca attacaacag
    10861 catgttgctc agccgtcctg ttaagaaaac gggttatatc gtcatcactg cagatggcgg
    10921 tttagtcggt ggatataaca gctcgatatt gaaacaaacg atgtctattt tagaagaaga
    10981 ccaccaatca cccgatgatt acgtgatgat cgcaattggc ggaactggtg cagacttctt
    11041 taaagcacgc ggtatcaatc tagcatatga gctgcgcaat ctatctgatc agccaagctt
    11101 tgatgaagtc cgcaaaatcg taaatatggc aacaactatg taccaaaacg aagtattcga
    11161 tgaattatac gtatgttata accatcatat caattcgcta actagtcagt ttcgtgtaga
    11221 aaaaatgctg ccaatctcag acttagatcc agaagaagca actactttcg aacaagaata
    11281 tatttttgaa ccttcaaaag aagaaatctt gtctcagtta ttgcctcagt atgctgaaag
    11341 tttgatctac ggtgcgatcg ttgacgccaa aacagcagaa catgctgctg gtatgacagc
    11401 aatgaaaaca gcaactgata atgctgctaa tatcattgat gagctgacgg tttcttacaa
    11461 ccgtgcaaga caaggggcta ttacccaaga aattaccgaa attgttgccg gagcttcggc
    11521 actagaatag tattgagatt ggaggaaaaa agatgagttc aggcaagatt gttcaagtaa
    11581 tcggtcccgt tgtcgacgtg gaattttctt tagaccaatc cttaccagat atcaacaacg
    11641 cattagttgt ttataaaaat gacgaaaata aatcaaaagt tgttcttgaa gcagcgttag
    11701 aattagggga cggagtgatc cggaccattg cgatggaatc aacagatggt ttacaaagag
    11761 gaatggaagt cattgataca ggcaaagcaa tctctgttcc tgtaggtaaa gaaacattag
    11821 gtcgcgtatt caacgtacta ggagatacga tcgatttaga aacacctttc ccagaagatg
    11881 cggaaagaag cgaaattcat aaaaaagcac cagcctttga cgaattaagt acaagtacag
    11941 aaattttgga aacagggatc aaagttatcg atttgcttgc cccatattta aaaggtggga
    12001 aagtcggact attcggtggt gccggtgttg gtaaaaccgt actgatccaa gaactgatcc
    12061 ataatatcgc ccaagaacat ggtggtattt ctgtatttac cggtgtaggt gaacgtactc
    12121 gtgaaggtaa tgacttgtat tatgaaatga aagattcagg agttatcgaa aaaacagcca
    12181 tggtgttcgg acaaatgaac gaaccaccag gtgcacgtat gcgtgttgct ttgactggtt
    12241 tgacgattgc ggaatatttc cgtgatgtag aaggtcaaga tgtactgttg tttatcgaca
    12301 acattttccg tttcactcaa gctggatctg aagtatcagc cttgttagga cggatgcctt
    12361 ctgcggttgg ttatcaacca acattggcaa cagaaatggg tcaattgcaa gaacgtatca
    12421 catctacgaa aaaaggttcg atcacatcga tccaagcaat ctatgtgcca gccgatgact
    12481 acactgaccc ggcgccggca acagcattcg cccatttgga tgcgacaacc aacttggaac
    12541 gtaaattaac agaacaaggg atttatccag cggtagatcc attggcttct tcttcaagtg
    12601 ctttagcacc tgaaatcgtg ggagaagaac attataaagt agcaacagaa gtccaacacg
    12661 tattgcaacg atatcgtgaa ttacaagata tcattgctat cttaggtatg gatgaattat
    12721 ccgatcaaga aaaagtgtta gtttctcgtg cccgcagagt ccaattcttc ttgtcacaaa
    12781 actttaacgt ggctgaacaa tttactggtc ttccaggttc atatgtccct gtagaagaaa
    12841 cagtgaaagg attccgcgaa atcttggaag gcaaatacga tgatcttcct gaagaagctt
    12901 tccgaagtgt tggccgaatc gaagacgtcg tagaaaaagc gaaaacacta ggctactaga
    12961 aaggggtgcc ccatggatca atatttaaca gtaaatgtgg taactcctgc cggtttggtc
    13021 tatgatcacc atgcagctat cgtcgttgca cgtacgacag ctggtgagct tgggattttg
    13081 cctaaacatg caccaatcat cgttccattg acgattgatg aagttcgtgt aaaacgaaca
    13141 gattcagata cccacgtcga ttggatcgct gttaatggcg gaatcatgga agttcgtgat
    13201 aatgttgttt caatcgttgc tgatagtgcg gaacgtgaac gtgatatcga tgtatctcgt
    13261 gcggaacgtg caaaacaacg cgcagaacgc caaatcgaag aagcaaaagc aaaacaagat
    13321 acgaacgaat tgaaacgggc aactgttgcg ttgcaccgag caatcaatcg tatcaaagta
    13381 tctaagcact cataaaatga gatgcggatg tttatccgtg tttaagaaca gtaaaaagag
    13441 gttatgacag aagtggtcag cttcaagaca taagaaggaa tttacgaaaa ttgcttctca
    13501 aatttttgtg aaatctcagc ttatttttcg aagaagctac ttctggaaca ccgtatatcg
    13561 gaggttgtga aatcagcgtt ttgtcctgag tattagagaa acaaatggaa aatagttcct
    13621 catattttgc cgttttttga gctaataccg agggtcagat gattgaacac cgtttatttg
    13681 gaataaggga ctgcgacaag acttttgtgg ctctttgtca ataaggactg atgagttgtg
    13741 caaaatcaaa tctgagtcag aatgaaccac attctggctc agattttttg ttatcttaca
    13801 ttgattaatt gattgatcaa aaagattcta attctcatgt tctcaaatga tt
//
LOCUS       DAJSBL010000065        13841 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.067, whole genome shotgun sequence.
ACCESSION   DAJSBL010000065 DAJSBL010000000
VERSION     DAJSBL010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13841
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.067"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(143..1345)
                     /locus_tag="ONT85_002357"
     CDS             complement(143..1345)
                     /locus_tag="ONT85_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR2379309.1"
                     /translation="MKTKYPCVYQDKNGRFSYLVELGTDQVTGKRIQKKGTKDSLGKR
                     FSSAREAHKEVMRIKNEYMVVNGFANYDLTYKEFMEQSYVPHYKASVQKSTWSSRECG
                     LIQITEFFGDKKLREINVRDCENYRIWLLNDSGYSQAYSSLMYGMFRKSLDYAVTLQF
                     LNENISKRTKAIPKGKSIVPYWTKEEFEEVLAKIYKDDFYEHMCFLMIWLYYMTGVRV
                     SEGLALNWNDVDFKRKKLRVHHTLEMTNQKDFIRKPYTKTESGLRIISLDDDTIEVLK
                     KWKVIQKNHGVEQFILSYTDLPLYRSTVQRIIQRYAKLAKVPLIQGKGLRHSHVSYLI
                     NEFNADILVVSQRLGHSSPEITLKHYAHLWSRNDEGIAEQMTGNIKFTCATKSKVANF
                     NGNQAVKI"
     gene            complement(1348..1542)
                     /locus_tag="ONT85_002358"
     CDS             complement(1348..1542)
                     /locus_tag="ONT85_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HCR2379310.1"
                     /translation="MKNTICRKDLEALGFKTHQATTIIRQAKTRMVNQGYPYYNNKRV
                     GVVPRQVIEEIIGVPLSQEE"
     gene            complement(1589..2437)
                     /locus_tag="ONT85_002359"
     CDS             complement(1589..2437)
                     /locus_tag="ONT85_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rep protein"
                     /protein_id="HCR2379311.1"
                     /translation="MQAYNQKIIETPTYIEIYDYEAIITPKRKTIENEVNLERKQKLA
                     WIYENDITLEKRKNYDELSAQGQYDSLKRKQKHYQSMRFEIARLVDTNFDNQTKFLTL
                     TFKENIQDIEYANNEFKKFIKRLNYDLYKTKKANLKYLATWEKQQRGAIHYHIILFDF
                     PFISFNRLTTIWGQGFVKINKIDVDSIENRGRYISKYFDKELELKEHKKKAFFKSRNL
                     KLPTVTKKMVKKPYDSTNQDILYTSEYIVRRPFFYNALALNELGSPEQVMDFQESTVH
                     YTKIKK"
     gene            complement(2642..2992)
                     /locus_tag="ONT85_002360"
     CDS             complement(2642..2992)
                     /locus_tag="ONT85_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2379312.1"
                     /translation="MPIKITDLSLPKETLEKEYQVVNVTRWQKEGEILGWSYECILPK
                     LRYEKISVKVKSEHPVITPEELTQQGLAIITFTNLVIVPWGRSNGQFVSYGLSATADT
                     ATLIPRKQGTTTQN"
     gene            3587..4033
                     /locus_tag="ONT85_002361"
     CDS             3587..4033
                     /locus_tag="ONT85_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HCR2379313.1"
                     /translation="MKDYVVQITKEVLEESKTLNKLSSKAKEEAVTILSSGIIDYIEM
                     SGEIKYGEKDRIAKMVKESIKTFDVKQDLTFNDSYLIGKLINILGTDEDTELFLSSLS
                     MIFTDKHFSTRFGGEELVEIFQSMRPALIKLYREKTPDEFYDWFEK"
     gene            4657..5649
                     /locus_tag="ONT85_002362"
     CDS             4657..5649
                     /locus_tag="ONT85_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCR2379314.1"
                     /translation="MYWEEIELEKTLGPGGEIRAVQRDKKNISKWEKLKKQLRRDKWM
                     YLLLLPGIIMTFIFKYVPMYGAVIAFKDYNPMLGILESPWVGLDHFREFISSPNFTLL
                     LSNTLKISIYGLLLGFFPPIILALSLNQIINKKIKQKIQLILYAPNFISVVIIVGMLF
                     LFLSANGPINGLVEKVTGQPIMFMSNPAYFRTIYILSGIWQGIGWSSILYTATLAGVS
                     TDLYEAANIDGANILQKIWHIDLPALRPVMVISFILAAGGIMNVGYEKAYLMQTSMNI
                     PASEIISTYVYKVGLQSGDYAYSAAVGLFNMIINVVLLLIVNNITKRLNDGQGL"
     gene            5665..6549
                     /locus_tag="ONT85_002363"
     CDS             5665..6549
                     /locus_tag="ONT85_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCR2379315.1"
                     /translation="MNSALSKIDRQILWLNRVIVTFLVVITIVPLIYVLVASFMDPAT
                     LINKGISFNPKDWTIQGYQRVFADDSILRGFVNSLFYSFSFSLLTVFITMITAYPLSR
                     PNLAGKKIIMTFFVITMFVGGGLIPTYLLVKNLGMLDSVWAIIVPGAINVWNIILART
                     YFSALPKELSEAATIDGANDLQILFKVMLPLAKPIMFVLFLYAFVGQWNSYFDAMIYI
                     KDPDLQPLQLVLRNILIQNQPSQDMVGANTAMAEMKQIAELIKYATIVISSLPLIIMY
                     PFFQKYFDKGVLVGSVKG"
     gene            6598..8205
                     /locus_tag="ONT85_002364"
     CDS             6598..8205
                     /locus_tag="ONT85_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR2379316.1"
                     /translation="MKAKWMLPIVFTSVLILGACGGSKEKSKSSPDYELENVSFPLKE
                     KVSLKMMSQSAPLAPNDPNDKLIFQRLEEETNLHIDWTNYSSDFAEKRNLDIASGDLP
                     DAIFNAAAGDYDLLNWAESGVIVPIEDLIEDYMPNLKKIFDENPEYRQMSTAPDGHIY
                     SMPWIEELGEGKESIHTVNGMAWINKEWLDNLGLEMPQTTDQLMTVLEAFKTQDPNGN
                     GEADEIPFSFINNGGNEDLKFLMGAFGLGDNDDHLVVDNDGQVQFTANKEGYKEAIKY
                     FNEMNNKGLIDKEAFEQDWNTYVAKGKEHKYGVYFTWDKANITGADDSYDVLPVLAGP
                     DGTKHVTRTNGMGFSRDRFVITSANKNLELTARWIDKMYEPLQSVQNNWGTYGDEAQA
                     NIFELKDGMLKHLPLEGTAPGELRQKTEAGGPLAVLDSYYGTVTTMPDDAKWRLDLMH
                     DTYLPYITEDNIYPRVFLEKEETDRLAKIETDMWDYIYRKRAEWITGGKIDEEWEEYL
                     AELERVGLEEWIQIKQTGYDNFLKGAQ"
     gene            8206..9690
                     /locus_tag="ONT85_002365"
     CDS             8206..9690
                     /locus_tag="ONT85_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016621147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HCR2379317.1"
                     /translation="MLVEETAILDKANRFIEMMRGKVNPQYRQNYHFSAPIGWINDPN
                     GFIYYKGHYHLFYQYYPYDSRWGPMHWGHARSSDLVHWEDLPVALAPDQPYDSGGCFS
                     GSAIERDGKLYLFYTGCQEIDGISHQHQCLAVSEDGIHFSKYSGNPVVAEKQLGENGL
                     IQEFRDPKVIVHNDGYYMVVATKSTQSTGRIVMFQSSDLYTWHFYSVLLEGTSEQGIM
                     WECPDLFHLDGRDVLIMSPIQIKKNGLEYHNISSTMACIGEVNWETGQMIVDNFHEMD
                     FGMDFYAPQTTRDPNGGRVMIAWMQMWDRTLPTHELGHLWAGSMSLPRELRVKKNRLV
                     QKPISTVYSHLVYQQGSENIAVTDTSVVFRNALSDSSYLHLVADLEKAEVFTIELLRS
                     KTDCLKLAYDTATQVFELDRQKLGRELTGAEEDPLNVRKVEVPLIDNQLVLEIFRDTS
                     SIEVFINGLEVMTATFYELEKARDILFHSIGESRIQAFETASVK"
     gene            9867..10859
                     /locus_tag="ONT85_002366"
     CDS             9867..10859
                     /locus_tag="ONT85_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005230454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR2379318.1"
                     /translation="MKATMKDVAKLAGVGVGTVSRVLNNGSVKASTREKVEAAMTELN
                     YQPDFYARGLKMKHTNTVALILPTVWHPFFGEFAYHVETALERQNYKLYLCNSSGNSE
                     KEQEYIQMVSQNKVDGIIGITYSDIDEYISSNLPFVSIDRYFTEEVIYVTADNAAGGR
                     LAANELYRRGCQNVAYIGGYQETPNETKSRRKYFEIECRQLGISYKALDMLEPLDHLS
                     ERVTNFLLENPDIDGIFTINDPMALAVIDMLEKIGKNVPEDVQVIGFDGQKMTEGDPY
                     LVSTIAQPTKQMAEVAVAKLIAVINGETVEKRTVLPIEFGEGDTTKPLVRNDFA"
     gene            10911..11780
                     /locus_tag="ONT85_002367"
     CDS             10911..11780
                     /locus_tag="ONT85_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HCR2379319.1"
                     /translation="MLYGGIEAGGTKFVCGIGNEQLQVIERKSFPTTTPEETMEQVFD
                     FFDKYKGNIASIGVGAFGPIDIQIESRTYGYITNTPKLAWQNYHFVGALENALSVPIY
                     WTTDVNAACYGEYMAGYGKGKNSVLYYTVGTGVGGGAVVDGKIVAGFSHPEMGHMLLQ
                     RHNEDQFEGICPFHHDCLEGLASGPAIEKRFGRKAQTISFDHPYWTIEADYLAQCVYN
                     TTLLYAPEVIILGGGVMKQPQLLELIRQSFKEKMGSYVDTPTLEEYLLTPRLADNAGL
                     IGCLALAIKKLNH"
     gene            complement(12200..13765)
                     /gene="guaA"
                     /locus_tag="ONT85_002368"
     CDS             complement(12200..13765)
                     /gene="guaA"
                     /locus_tag="ONT85_002368"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HCR2379320.1"
                     /translation="MSSPTRFDEEPKIIVLDYGSQYNQLITRRIREFGVFSELMSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDEKAFGIDEKIFDLGIPVLGICYGMQLITYKLGGK
                     VKPAKNREYGKAELEITSAEAALFQNTPEKQTVWMSHGDLVTQAPTDFDVVATSKDCP
                     IASIQNAERKMYGIQFHAEVRHSEYGNDLLRHFTLDVCQCTGDWTMDNFIDMEIAKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTCIFVDHVLLRKGEAEQVMESLGGK
                     FGLNIIKVDAKERFLSKLAGVSDPEEKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFKLIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGELTEEKLEIVRESDAILREEIANAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
BASE COUNT         4241 a         2343 c         2856 g         4401 t
ORIGIN      
        1 ttgaaaaacg ttgatttatc ggcattcccc atccatatta tatttacgtc ttattcccac
       61 tcaatatgag tagcctcgta aagaagctat tatatcagtg ctttttcagc ctattcctca
      121 ttttggtagg gaaatggtag ggttagattt ttactgcttg atttccatta aagtttgcaa
      181 ctttagattt tgttgcacag gtaaatttaa tgtttcctgt catttgttct gcaattcctt
      241 catcattacg gctccataga tgagcgtagt gctttaaagt gatttcaggg ctagaatggc
      301 ctaaccgttg cgacacgacc agaatgtctg cgttaaactc attgattagg tagctaacat
      361 gagaatgacg taatcctttt ccttgtatta ggggtacctt agctagtttg gcataacgct
      421 gaataattcg ttgaacagtt gaacgataga gtgggaggtc agtgtagcta agaataaatt
      481 gttctacacc atgatttttt tgaataactt tccacttttt tagcacttca attgtatcat
      541 cgtccagaga aatgatccgc aagccgcttt cagtttttgt gtaaggtttc cgaataaaat
      601 ctttttgatt tgtcatttcc aaggtgtgat gaaccctcaa ctttttacgt ttgaagtcca
      661 cgtcattcca attaagtgct aatccttcgc ttacacgaac accagtcatg taatatagcc
      721 agatcattaa aaaacacata tgttcgtaga aatcatcttt ataaattttt gccaaaactt
      781 cttcaaattc ttcttttgtc cagtaaggaa cgattgattt tccttttgga attgcttttg
      841 ttcttttgga aatattctca ttcagaaatt gcagggtgac cgcatagtct aatgatttac
      901 gaaacatacc gtacataagt gaactgtatg cttgtgagta tccgctatcg ttcaataacc
      961 aaattcgata attttcacaa tctcgcacat tgatttcacg aagcttctta tcaccgaaaa
     1021 attcagtaat ctgaatcaat ccacactcac gagaactcca agtacttttt tgtacgctag
     1081 ctttgtagtg tggaacgtaa gactgctcca taaattcttt gtaagttaaa tcataattcg
     1141 caaatccatt tacaaccatg tattcatttt ttatacgcat gacttctttg tgtgcttcac
     1201 gagctgatga gaatcgtttt cctagagaat cttttgttcc tttcttctgg atcctctttc
     1261 cagtcacttg atctgttcct aactcaacta gataagaaaa gcgaccattt ttatcttgat
     1321 acacacaagg gtattttgtt ttcatagtta ttcctcctgt gaaagaggaa cacctataat
     1381 ttcttcaatt acttgtcggg gtactacccc aactctttta ttattataat atggatatcc
     1441 ctgatttacc attcttgttt ttgcttgtcg gattattgtg gtagcttgat gtgttttaaa
     1501 tcctagagcc tctaaatctt ttcgacaaat tgtatttttc attgtataaa ctcactttca
     1561 aagaaaacac tatcattggg taatagtgtt atttctttat ttttgtataa tgaacggtac
     1621 tttcttgaaa atccatcact tgttcaggac ttcccaactc atttaaagct aaagcattgt
     1681 aaaaaaatgg acgtctaact atatattcgc ttgtatatag tatatcttga ttcgtggaat
     1741 catagggttt ctttaccatc tttttggtga cagttggtag tttgagattc cgggacttga
     1801 aaaaagcttt tttcttatgc tctttcaatt ccagctcttt atcgaaatat ttgctgatat
     1861 agcgcccgcg attttcgata ctgtctacat cgatcttgtt gatcttaacg aaaccctgac
     1921 cccaaatcgt agttaaccgg ttaaatgaga taaaggggaa atcaaacaag ataatatggt
     1981 agtggattgc accgcgttgc tgcttttccc acgtggcgag atactttaaa tttgcttttt
     2041 tagttttata gaggtcataa ttcagtcgtt tgataaattt tttaaattca ttatttgcat
     2101 actcaatgtc ttgaatgttt tctttgaaag tgagagttaa aaatttcgtt tgattgtcaa
     2161 aattcgtgtc tacaaggcgg gcaatctcaa atcgcatact ttgataatgt ttctgtttgc
     2221 gttttaaact gtcatattgt ccttgagcgg ataattcatc atagtttttt cttttttcaa
     2281 gagtaatatc attttcataa atccaagcta atttttgttt tcgttccaga tttacttcgt
     2341 tttcgatcgt tttacgtttg ggagtaatga ttgcttcata atcataaatt tcaatatacg
     2401 tgggagtttc aatgattttc tgattataag cttgcaaaaa tcatcactcc ttgttgcgta
     2461 ttcgattgtc aaagaaccag tcattttctc ctgatgttga tctataaatc aagttaaggg
     2521 ggaaggcctg cgtagcagcc cttctagcct atggggtgtt gggcggtctt aacggacaga
     2581 aacggctctg tgggcgctgc cccgccgttt cgtcctaacc acccaacact ccatagtata
     2641 gttagttttg tgtagtggta ccttgttttc tgggaatcaa agttgccgta tcagcggttg
     2701 ccgataaccc ataactgaca aattgtccat tcgagcgtcc ccaaggtaca atcaccaaat
     2761 tggtaaatgt aattatggct aaaccttgtt gggtaagttc ttccggcgtg atcacaggat
     2821 gttcagattt gactttgaca ctgatttttt cgtagcgaag ttttggtagg atacattcgt
     2881 agctccaacc tagaatttcg ccctcctttt gccagcgagt gacattgacg acttggtatt
     2941 ctttttcgag tgtttctttt ggtaaactga gatcggtaat ttttattggc atcacaattc
     3001 tcctttttta tacgaaagtt tatacgattt gattaaaaaa tatagatggc catctacgac
     3061 ttgtattata tacgagaact tatacgtagt caagagattt tctaaaaaaa tatatacgat
     3121 tttttatacg agtgttataa tgaaaaaaat gataaggagg aatttgaata tgtttggttt
     3181 agtacaacct aataaagtga aggtgggcca acgaatcaaa gaaataaaag acggaatgtc
     3241 tctctctttt acagaattag ggaatcgatt aggtttaaaa aagccgacga tcagttccta
     3301 cgttcaaggc tatgcgttgg ctcctaagag tgtgatcaac caattatcta gtatcagcgg
     3361 aaaacctgtc ggctggtttt attttgggca tgtggaagaa tatatccgag agtatttgtt
     3421 tctcattggt cagaaaaaaa tagtggacga ttatcctgaa atcattgaga aaataaagca
     3481 agaattttat acgggcgagt tcaaaaatcc tgcttgggag aatgagctag gctatccttt
     3541 ggaggaattt atccaagaat gttttgtaga atattcgagt gaaatcatga aggactatgt
     3601 cgtacagatt acgaaagagg tacttgaaga atccaaaacg ctaaacaaac tatcatcaaa
     3661 agcaaaagaa gaagctgtca cgattctttc atctgggatc attgactata tcgagatgag
     3721 tggtgagatc aagtatggag agaaagatcg cattgcgaaa atggtgaaag aatcaataaa
     3781 aacctttgat gtgaaacaag acctgacatt taatgattcc tatttgattg gaaagttgat
     3841 caatattctt ggtacagatg aagatacgga gctgtttctt tcttctttat caatgatatt
     3901 tactgataag catttttcca ctcgatttgg tggggaagaa ttagtcgaaa tatttcaatc
     3961 gatgcgacca gcgttaatca aactctatcg ggagaaaaca ccagatgaat tttatgattg
     4021 gtttgagaag taaaataaga aaagtggctt agattacaag ttggtgaaaa aatttttttc
     4081 agcataacat cagtaatcta taaaggggtt gttgattata ctgtagaagt taagtactca
     4141 agagattttt tgtacgtaaa caaagcaaaa aagggtgcag tttcactaga aaatgtactc
     4201 ttttttgtga tttttcttag ttatagagtt tttttgaaac tttaaaaatg agaaaacata
     4261 ttgattttaa agagtgtaga catagcttct tttgctaaat agattaaact tactggtgag
     4321 tggtcttgaa catactttta aatcacattt tactgagttt tattggattt tataattcat
     4381 tggaaacctc gtacttaatc tgtttttatc tatcgactag aatctgtata tattccagct
     4441 atcaatgagg ccgcagaaaa gggtgcttaa aaataatctg ttgtttaaaa tattattatc
     4501 agaatactta gatacaaaat tctggcacaa agtaaaaaaa atattgactt aaaaaaatgg
     4561 atatggtaat ctataaacga aaatagtggt aacgtttcca aaatgcatct tcgttaaatt
     4621 tttatttttt taaacgaaaa tggaaacgtt tctaaaatgt attgggagga gattgaattg
     4681 gaaaagacat tgggaccagg tggtgagata agggcggtac aacgggataa aaaaaatatt
     4741 tcgaagtggg aaaaattaaa gaaacagttg cggcgtgata agtggatgta cttgttgctc
     4801 ttaccaggta tcatcatgac gtttatcttt aagtatgtgc caatgtacgg tgctgtgatt
     4861 gcatttaaag actataatcc tatgctagga attttggaaa gtccttgggt aggactagat
     4921 cactttaggg aatttatcag ttcaccaaat tttacgcttt tgctgagcaa cacattaaaa
     4981 attagtattt atggtttatt gttaggtttt tttccaccaa ttattttagc actgagcttg
     5041 aatcaaatta tcaataaaaa aataaaacag aaaatccagt tgattctata tgcgccgaat
     5101 tttatctcag tagttattat tgtggggatg ctgtttttgt tcctatccgc aaatggacct
     5161 attaatgggt tagtagaaaa ggttacgggg caaccaatta tgttcatgtc taatccagct
     5221 tatttcagaa cgatttatat tttatcggga atttggcaag gaatcggctg gtcatccatt
     5281 ctttacaccg caacgctggc gggagtcagt accgatttat atgaggcagc aaatatcgat
     5341 ggggcaaata ttttacaaaa gatttggcat atcgatttgc ctgcattgag gcctgtgatg
     5401 gtgatcagct tcatcttggc cgccggcggt attatgaatg taggatatga aaaagcctac
     5461 ttgatgcaaa cttcaatgaa catccctgcc tcagaaatca tttccactta tgtatacaaa
     5521 gtcggcttgc aatcaggtga ttatgcctac tctgctgctg ttggtctgtt caatatgatt
     5581 attaatgtcg ttttattact gattgtcaac aatataacca agcggctaaa cgatggtcaa
     5641 ggcttgtaat gaaaggagaa aattatgaat agtgctttat caaaaatcga ccgtcaaatc
     5701 ttatggttga atcgagtgat tgtaactttt ttagtcgtca tcaccattgt tccgctgatt
     5761 tatgtactgg tagcttcttt tatggaccca gctactttga tcaataaagg tataagcttc
     5821 aatccgaaag attggacaat acagggatat caacgggtat ttgcagatga ttccatttta
     5881 cgcggttttg taaattcgtt gttttactct tttagcttca gcttattgac tgtatttatt
     5941 acgatgatca ctgcgtatcc tttgtctcga cctaacttgg cggggaaaaa gattattatg
     6001 actttttttg tcattacaat gttcgtgggt ggtggcttga tacctaccta tttattggta
     6061 aagaacttgg gcatgttaga tagtgtatgg gcgattatcg taccaggtgc tataaatgtg
     6121 tggaatatta tcttagcccg tacctatttt agtgctttgc caaaagaact ttctgaagct
     6181 gctactatcg atggcgccaa tgacttgcaa atactgttca aagtcatgct tccattagcg
     6241 aaaccaatta tgttcgtatt gtttttatat gcctttgttg ggcaatggaa ctcatacttt
     6301 gatgccatga tttacatcaa agatcccgac ttacagccgt tgcaactcgt attaagaaat
     6361 attttgatcc aaaaccaacc ttcacaagac atggtgggag ccaatactgc catggcagaa
     6421 atgaaacaaa ttgctgagtt gatcaaatac gcaacgatcg tcatttctag tctgccatta
     6481 atcataatgt atcctttctt ccaaaaatat tttgataaag gtgttttagt tggttctgtt
     6541 aaagggtaag tattgttcac tatttgtatg aataaaaatt ataaaggaga ttttaagatg
     6601 aaagcaaaat ggatgctgcc aatcgttttt acttccgtac taattttggg ggcttgtgga
     6661 ggctcaaaag aaaagagtaa atcaagccct gattatgaac tagaaaatgt ctcttttcca
     6721 ttgaaagaaa aagtatcttt aaaaatgatg tctcaaagtg cccctttggc tcccaatgat
     6781 cctaatgaca aattgatttt ccaacgatta gaagaagaga ccaatctcca tattgattgg
     6841 acaaactaca gtagtgattt tgccgaaaaa agaaatttgg atatcgctag tggtgattta
     6901 ccagatgcca tcttcaacgc cgctgcaggt gactatgatt tattaaactg ggcggaaagt
     6961 ggcgttattg taccgattga agatttgatc gaagattata tgccaaatct gaagaaaatt
     7021 tttgatgaaa accccgaata ccgacaaatg tctactgcac ctgatggtca catttactct
     7081 atgccttgga ttgaagaatt aggcgaagga aaagagtcga ttcatacagt aaatgggatg
     7141 gcttggatca acaaggagtg gttggataat ttgggtcttg aaatgccgca aaccactgat
     7201 cagttgatga ctgtgttgga ggctttcaag acgcaagatc caaatggtaa tggtgaagca
     7261 gatgaaattc cttttagctt tattaacaat ggaggaaacg aagacttaaa atttttaatg
     7321 ggggcattcg gtctaggtga taatgatgac cacttagtcg ttgataacga tggtcaggta
     7381 caatttactg ctaacaaaga agggtataaa gaagccatca aatatttcaa tgaaatgaat
     7441 aataaaggtc ttattgataa agaggctttc gaacaagatt ggaatacgta tgttgccaaa
     7501 gggaaagaac ataagtatgg cgtgtacttt acttgggata aagcaaatat cacgggtgca
     7561 gatgatagtt atgacgtctt gccagtctta gcgggaccag atggaacgaa acatgttact
     7621 cgtacgaatg gcatgggttt ctcaagagat cgtttcgtaa ttactagtgc gaataaaaac
     7681 ttggaactta cagctaggtg gatagataaa atgtatgaac cgttacagtc tgtccaaaat
     7741 aactggggaa cctacggtga tgaagcacag gcgaatattt ttgaattaaa ggatggtatg
     7801 ttaaaacatt tgcctttaga ggggacagca ccgggggaac taagacaaaa aaccgaagct
     7861 ggaggaccat tagcagttct tgattcttat tacggcacag tcactactat gccagatgat
     7921 gcgaagtggc gtttagattt gatgcatgac acgtatttgc catatattac agaagacaat
     7981 atctatccac gtgtcttttt agaaaaagaa gaaacagatc gattagcaaa aatcgaaacg
     8041 gatatgtggg attacatcta tcgcaaaaga gcagagtgga tcaccggcgg taaaatcgat
     8101 gaagaatggg aagaatacct tgctgaattg gaacgtgtag gtttggaaga atggattcaa
     8161 ataaaacaaa ctggctatga taattttttg aagggagcac aataagtgtt agtagaagaa
     8221 acggcgattt tggataaagc aaatcgattt attgaaatga tgcgaggaaa agttaaccct
     8281 cagtatcgac aaaactatca tttttccgca cccatcgggt ggataaatga ccccaatggg
     8341 tttatatatt acaaagggca ttaccatctc ttctatcaat actatcctta cgatagtcga
     8401 tgggggccca tgcattgggg gcatgcgcgc agtagtgatt tggtccattg ggaagatcta
     8461 ccagtagcgt tagccccaga tcaaccttat gacagcggcg ggtgtttctc aggaagtgca
     8521 attgaaagag acggaaaact atatttattt tatacaggct gtcaagaaat tgatggtatt
     8581 agccatcagc atcaatgttt ggcggtctct gaagatggta ttcattttag caaatacagt
     8641 ggtaatcctg tggtagcaga aaaacagcta ggtgaaaatg ggctaataca agaatttcgt
     8701 gatcctaaag taattgttca caacgatggt tactatatgg tagtagctac taaatctaca
     8761 cagtctactg gtagaatagt gatgtttcaa tcgtcagatt tgtatacttg gcacttttac
     8821 tctgtgctgc tagaagggac ttcagagcaa ggaataatgt gggaatgtcc tgatttattc
     8881 catttagacg gacgagatgt cttaatcatg tctccgatac aaatcaagaa gaacggactg
     8941 gaatatcata atatcagttc taccatggct tgtattggtg aggtaaactg ggaaacaggt
     9001 cagatgatag ttgataattt ccatgaaatg gattttggaa tggattttta tgcaccacaa
     9061 acaacaagag atcctaatgg tggacgggtg atgattgctt ggatgcagat gtgggatcgt
     9121 acattgccta cacatgagtt aggccatctt tgggcaggct ctatgagttt gccccgagag
     9181 ttaagagtga aaaaaaatcg attggtacaa aaacccatct caactgtata tagtcattta
     9241 gtttatcaac aaggcagtga aaatattgca gtgactgaca catcagtcgt atttcgaaac
     9301 gcgctgtctg attcttcata tttacatcta gtcgcagatt tggagaaagc tgaagttttt
     9361 acaattgaat tactgcggtc taagacggat tgtctcaaac tagcctatga cactgctaca
     9421 caggtttttg aattggatcg tcagaaacta ggaagagagc ttacaggggc ggaagaagac
     9481 cctctgaatg tacgaaaagt tgaagtacct ttgattgata atcagctcgt tcttgagatt
     9541 tttagagata cctcatcaat tgaagtcttt attaatgggt tagaagttat gacggctact
     9601 ttctatgagc tggaaaaagc tcgagatatc ttgtttcatt cgatcggaga aagtcgtatt
     9661 caagcttttg aaacagcgag tgtgaaataa atgtttgatt gcttaggcga tgaatcaatt
     9721 atttaatagg ttttgaagga taatgttttt agaaaagaac agatagctag atttcccatg
     9781 gatttgctat tagtattttg aaaaaattag taatttatga caaaattcat tatactaaac
     9841 ttgtgcagaa atgtgaggga tcaactatga aagcaacaat gaaagatgtc gcaaaattag
     9901 caggtgttgg agtaggaact gtttcccggg tattgaataa cggttctgtt aaagcttcta
     9961 ctagagaaaa agttgaagca gcaatgacag agttaaacta tcaaccagat ttttatgccc
    10021 gggggttaaa gatgaagcat acgaacacgg ttgctttgat tctacccacg gtatggcatc
    10081 cattttttgg cgaatttgcc taccatgtag aaacagcatt agaaagacaa aactacaagc
    10141 tttatttgtg taactccagt ggtaatagtg aaaaagaaca agaatatatc cagatggtca
    10201 gccaaaataa agtagacggc atcattggaa ttacttattc agatatcgat gaatacattt
    10261 cgagtaatct gccttttgtt agtattgacc gttatttcac tgaagaggtg atttatgtga
    10321 ccgctgacaa tgcggccggt ggacgtttgg ctgcaaatga actttatcgc cgaggatgtc
    10381 agaatgtagc atatatcggt gggtaccaag aaacaccgaa tgaaacgaag agtcgtcgaa
    10441 aatattttga aatcgagtgc cgtcaactgg ggatttccta taaagcgtta gatatgttag
    10501 aacctttaga ccatctctca gaacgtgtaa caaatttttt gttagagaat cccgatatcg
    10561 atgggatttt tacgatcaac gatccaatgg cattagctgt cattgatatg cttgaaaaaa
    10621 tagggaaaaa cgtaccagag gatgtccaag ttatcggatt tgatggtcaa aagatgacag
    10681 aaggagatcc ttatctagtc tcaacgattg cccaacctac aaagcaaatg gcggaagtgg
    10741 cagtggcaaa attaattgct gtcattaatg gagagacagt agaaaaacgt accgtattgc
    10801 cgattgaatt tggcgaaggt gacacaacga aacctttagt gagaaacgat tttgcttagt
    10861 gcaaatacgt ttcttttttt gaaaatgagt gaatgaaagg tgtagataaa atgttatatg
    10921 gtggaataga agccggtgga acaaagtttg tctgtgggat tggtaatgaa caattacaag
    10981 tgatagaacg gaaaagtttt cctaccacaa caccagaaga aacgatggag caagtgtttg
    11041 atttctttga taagtacaaa ggaaatatcg catcaattgg tgtaggagca tttgggccta
    11101 ttgatattca aatcgaatca agaacttacg gatatatcac caacacaccg aagttagctt
    11161 ggcaaaatta tcattttgta ggagctttgg aaaacgcctt gtcggttccc atctattgga
    11221 ctaccgatgt aaatgctgct tgttatggag aatatatggc agggtatgga aaaggtaaaa
    11281 atagtgttct gtattacacc gttggtacag gtgttggtgg tggtgctgta gtggatggga
    11341 aaatagtcgc aggttttagt catcctgaaa tgggacatat gctcttacaa cgtcataatg
    11401 aagatcaatt tgaaggaatc tgtccttttc atcatgattg tttggaaggg ttagcatcag
    11461 gacctgcgat tgaaaaacgc tttggaagaa aagcccagac tatctcattt gatcatcctt
    11521 attggacgat tgaggcagat tatcttgctc agtgtgtgta taacacaacg ttactatatg
    11581 caccggaagt aataatttta ggcgggggtg taatgaaaca gccgcaacta ctcgagttga
    11641 ttcgccaatc gtttaaagaa aaaatgggtt cctacgtgga tacgcctact ctcgaagaat
    11701 atctactaac tccaaggctt gctgacaatg cgggcttgat tggctgccta gctttagcaa
    11761 taaagaaact caatcattag aatagtattt attagaacta ttcataacat accggaagtt
    11821 taaaagggta ttctgaaact taaatagtct caattcattc ttctttcttg agaatctgag
    11881 tagagacttt tattctgttt caaattaata taattacgaa atgatattga gtacaagaaa
    11941 gttcttttta ccagatcttt taaatcgaaa aatctgatat atatatttat agtactaaaa
    12001 atctgattag ataaaaagct aattcaatag gaaaataaag gtagtgaata aaagcaaagc
    12061 ccttgttagt agttaatcta acaagggctt tggtagggaa aatggtagga aaccatctta
    12121 ttttttacga aaatcaggaa ctttagtaat tgaataatgt tgatttatag gtattcctca
    12181 tccatattat gttggctctt tattcccact ccactgttgc aggtggtttg ctggtaatat
    12241 catacacgat acggttaacg tgtgcgactt catttacgat acggacactg attttttgca
    12301 agacttccca agggatacgg gcaaagtcag cggtcatgcc gtcaatagaa gtgacagcac
    12361 ggatacctac agtgtagtca tacgtacggc catctcccat aaccccaaca gagcgaatgc
    12421 ctggtaggac ggtgaagtat tgccagatgt cgcgatctaa accggcattg gcgatttctt
    12481 cgcgcaagat agcgtctgat tcacggacga tttctaattt ttcttctgtc aattcaccaa
    12541 ggacacgaat acctaagcct ggtccaggga atggttggcg ccaaacgatg ctgtcaggca
    12601 tgcctaattg ggtacctaat tcacgtactt cgtctttgaa caatgtgttc aatggctcga
    12661 tcagcttgaa ttgcatgtct tcaggtaatc cacctacatt gtgatgtgat ttgatggttt
    12721 gggcagtttc agttcctgat tcgatcacat cggtataaag ggttccttgt gcaaggaaag
    12781 aaacgccttc ttcaccggca agtttggttg cttcatcatc gaagacatat acaaattcat
    12841 ttccaatgat tttacgtttt tcttctggat cagacacgcc agcaagtttt gataagaaac
    12901 gttctttggc atcgactttt ataatgttca agccaaattt tccgccaaga ctttccataa
    12961 cttgttctgc ttcaccttta cgcaacaata cgtgatcaac gaagatacat gttaattgat
    13021 cgccgatcgc tttttgtaaa agaacaccaa caacagagga atctactccg cctgaaaggc
    13081 ctaataaaac ttttttgtca ccgacttgtt cacgaatttt tgcgatctcc atgtcgatga
    13141 agttatccat tgtccagtca ccagtacatt ggcagacatc tagtgtaaaa tggcgcaaca
    13201 agtcatttcc atattcggaa tggcgaactt ctgcatggaa ttggattccg tacattttgc
    13261 gttctgcatt ttggatcgat gcgatggggc aatctttgct ggtagcaacg acatcgaaat
    13321 cagtcggtgc ttgggtaact aggtcaccat gactcatcca aacggtttgt ttttcaggag
    13381 tgttttggaa aagggccgct tctgcagaag tgatttccaa ttctgctttt ccgtattcac
    13441 ggtttttggc tggtttaact tttccgccta atttataagt aatcaactgc ataccgtaac
    13501 agattcctaa aactgggatc cccaaatcaa aaatcttttc atcaatacca aaggccttct
    13561 catcataaac actgtttggt ccgccagaaa ggatgattcc tttagggttc attgccttta
    13621 cttcttctgc ggtaatgcgg tggctcatca actctgagaa aacaccgaac tcacgaatac
    13681 ggcgagtgat cagttggtta tactgactac cgtaatcaag tacgataatt tttggctctt
    13741 cgtcaaatcg tgttggtgaa gacaaatttg aataaaactt agtccgatat gcggcgcgtt
    13801 ctgtgcgccg catatttttt tagttttcaa agatcagacc t
//
LOCUS       DAJSBL010000066        13809 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.068, whole genome shotgun sequence.
ACCESSION   DAJSBL010000066 DAJSBL010000000
VERSION     DAJSBL010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13809
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.068"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            137..475
                     /locus_tag="ONT85_002369"
     CDS             137..475
                     /locus_tag="ONT85_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2379321.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(680..1255)
                     /locus_tag="ONT85_002370"
     CDS             complement(680..1255)
                     /locus_tag="ONT85_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HCR2379322.1"
                     /translation="MCGRYFFDLESELLQNYYREAQTKQPKQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLMINARAETVEEKKTFHQPFLQNRCVFPMSGF
                     YEWNEEKDKFMFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRMPLVL
                     TEEQIEPWVTDISFARKIITQQMPELVMEKV"
     gene            complement(1987..2568)
                     /locus_tag="ONT85_002371"
     CDS             complement(1987..2568)
                     /locus_tag="ONT85_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCR2379323.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(2751..3377)
                     /locus_tag="ONT85_002372"
     CDS             complement(2751..3377)
                     /locus_tag="ONT85_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HCR2379324.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(3399..4730)
                     /locus_tag="ONT85_002373"
     CDS             complement(3399..4730)
                     /locus_tag="ONT85_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HCR2379325.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(4885..5286)
                     /locus_tag="ONT85_002374"
     CDS             complement(4885..5286)
                     /locus_tag="ONT85_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HCR2379326.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(5372..5578)
                     /locus_tag="ONT85_002375"
     CDS             complement(5372..5578)
                     /locus_tag="ONT85_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2379327.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(5673..7175)
                     /locus_tag="ONT85_002376"
     CDS             complement(5673..7175)
                     /locus_tag="ONT85_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HCR2379328.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(7193..7648)
                     /gene="argR"
                     /locus_tag="ONT85_002377"
     CDS             complement(7193..7648)
                     /gene="argR"
                     /locus_tag="ONT85_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HCR2379329.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(7746..8363)
                     /locus_tag="ONT85_002378"
     CDS             complement(7746..8363)
                     /locus_tag="ONT85_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HCR2379330.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKPFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQLVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            8584..9957
                     /locus_tag="ONT85_002379"
     CDS             8584..9957
                     /locus_tag="ONT85_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR2379331.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLILSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            10032..11144
                     /locus_tag="ONT85_002380"
     CDS             10032..11144
                     /locus_tag="ONT85_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HCR2379332.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWSTEDYQKDISTYITKNPS"
     gene            complement(11216..12853)
                     /locus_tag="ONT85_002381"
     CDS             complement(11216..12853)
                     /locus_tag="ONT85_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HCR2379333.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(13021..13629)
                     /locus_tag="ONT85_002382"
     CDS             complement(13021..13629)
                     /locus_tag="ONT85_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HCR2379334.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
BASE COUNT         3744 a         2896 c         2465 g         4704 t
ORIGIN      
        1 taaaaaaata atccaagaaa cattgaacct atggatccaa aagctaccca aatactatag
       61 ctaacccccc gctaccttca attttgcaga taaaacgctt tgggagaaaa tttcaaagac
      121 aacaggccat cctatcatgc ccgattataa acaaaaagct ggctattcct tttgctctga
      181 caaatttcaa ttgggaaagt tcacgaaact agcaggtgaa gaagtttcaa ctgttcggat
      241 caaagaatgc ccgcttcaaa tagaaacatt ggttcaacga atttcaacta gacaagactt
      301 tgctattgta gaacgtgaga tcgtagctat ttttgtagaa gaagggattt tacatgatcc
      361 aactcatatc aatgtggata aatggcaacc tcttatctat aaattcagag aatatacaac
      421 tgccaaccag tcgttaactt tgaattttag attccaagaa ttcaaagagt cataatgata
      481 aacatcgtta ggtataaata aaaaagactc gcagcacgag taaaaaacaa atagaagcta
      541 tacattagaa aggtcgactg tgaccctata gatgcagtat atgcagctta tgagcatttc
      601 ttctgttctc tttgtttttg ggatctgtgc tgaagtcttt ttcgtttttt gccgctcttt
      661 ctttgaaaaa tcgattatgt tataccttct ccattactaa ttcaggcatc tgctgagtaa
      721 tgatttttcg cgcaaaagaa atatctgtta cccaaggttc gatttgttct tcagtcaaga
      781 caagcggcat tcggtcatgg attggtgcga ctgtgcggtt aggttcagta gtcattatga
      841 ttgattctgc cattttttca tcctgatggc tacgataaaa accgccaata tacactattt
      901 tttccttatt tgaaaacata aatttgtctt tttcttcatt ccactcataa aagccactca
      961 ttgggaaaac acaacgattt tgcaaaaatg gttgatgaaa ggtctttttt tcttctactg
     1021 tctctgcacg ggcattaatc atcaattgtc cttttttaaa tccggtaaat ccccacttca
     1081 tgatttccgg agtgaggatg ttttcttcct cttttcttaa cgtcacaaca agatttgacg
     1141 gaaaaacttc tccagctgct aattctgctg cctgcttcgg ttgtttcgtc tgggcttctc
     1201 gataataatt ctgaaggagc tctgactcta gatcaaaaaa ataacgacca cacatcctaa
     1261 tccctcccaa tctttttttc tatcatacac tgcaacaaga agttcgcacc aacaaaacgc
     1321 tttcaatttt tgtagaacta aaaaagttgt gacaagtgac aaactctcca gtgaatggta
     1381 ttctcctgcc aactcctcgg tcacaagtca caatattcaa caaacatgag aagctgcttt
     1441 ttgaaattgt tccttgtgac tcggagtgaa acggcaggct tacgacctct gggtaaacgg
     1501 tgttcaaaag ccagtttctc ggtattaagc caaatttatg caaaaatgta gaaagccatt
     1561 ttcacaaaat ttttcttaat actcgaaact aagcgacttt ttcacaacct cttccttaaa
     1621 cggtgttcaa aagcttgcat ctgcggtaat aagcccaacc aaacgaaaaa tatgagcagc
     1681 tatttttatt tggctgttct tattacttga tgcagggcag ctttttcaca acctctccac
     1741 taaacggtgt tcaatcagct aaccttcggt attagctcaa aaaacggcaa aatatgagga
     1801 actattttgc cgtttgtttc tctaatactc aggtcaaagc gctgatttca caacctctaa
     1861 tacacggtgt tccagaagta gcttcttcga aaaataagcc gaaatttcac aaaaatttga
     1921 gaagcaattt tcgtaaattc cttcttattt cttgaagctg accacttctg tcacaacctc
     1981 taattattaa agaacctttt gcgaagttaa caatctttct ttgatcaatt gaaaattctg
     2041 cctaagtagt gatagatcct gtgtgacttt caacaataag atacttccct ggatttcagc
     2101 aatcaacgct ttggcttgac ggtctgcttc ttcatcggac agtttttctt cagaaagtgc
     2161 caggatattt tgtttcgtat acttgaccca ttgattgact aattctagta gcaacagacg
     2221 gtattcttca tccactgtac tcaactcaat aatcaaatta cctacaggac agccatgata
     2281 agtgatctgt gtctcatgat cagcgaaaat ccagtccaac atttcttcta atgcttcttc
     2341 tggctgtttt ccagcattta agatgtcttc gaacaattct gtctgccata cgtccatatg
     2401 cgcatggatc accgctagac aaacttcttt ttttgaagaa aagtaataat aaaattgacc
     2461 ttttcctgta tcagaagctg ctagaatatc tcggattgac gtttcatgat agcctttttt
     2521 ataaataaga tctttggtcg cttccaagat cttttccttt gttttcaatg atattctcct
     2581 ttttcatttc tcatagtcct atattataca gtaaattcat cattttttct tcttaatcgt
     2641 ttaaaaaaga ctgtgccaaa ttatctgaca cagccctcac tataaaactg ttctcatata
     2701 gctagatgtt cattgattca agaactgcga ggacaaaagc aattcctatc ttatgatagt
     2761 tgctctaata cttcctctgt tgtcatcact tgatctgcaa taaagccaaa gttcgtcaac
     2821 gctgcatcca ttgcttcttg gccaggagca ccaattgcat ctgtcgctac cacaacattg
     2881 aatccactct ctaccaattc acgcatatgg gaatcgctac acaagtttcc gttcattcca
     2941 ccaagaatta ctgtgtcgat tccatttttt ctcaattgga gcgccaaatc attgctttct
     3001 ggtccaaaga ttttatgagg agagctgatg atcgtatttt catccaaata aggtttcaat
     3061 tcttcgataa aatcggctcc tgaaccttct tccactgatt catattgact ttttctagca
     3121 aacatctgct gctcaagcat catatgttct cctgcaccgc caaattgcca tttatggtca
     3181 tgtgggtaaa aataatgagg agaaataaag agtttgtatc ctttttcttt tgcttgtttt
     3241 accatttcaa tcatattttc gatcgtattg tatttttcta agatatcttt tgtcaaacca
     3301 aagccaccgc ctgctggttt caagaactcg acttgcgggt cagtgagaac tactgctgta
     3361 tttttattat tgaacatggt ggattctcca ttcgttattt attttaatac atcatttaga
     3421 gcttcactca ttgtcggatg agaatagatt tgatctctca ataacgtata aggaagtttt
     3481 ccattcatag ctagcgaaat caagttgatg acttcatgag aatcttccgc atagatactt
     3541 gcccctagaa tcaagtcagt ttcagggtcg actaaaactt tgaatacacc tttaggatct
     3601 tctaatactt gtgctttagg gacaccgctt gtcatatact tgaataattt gtaattgacg
     3661 ccttgtgctt tggcttgttt ttctgttaaa cctacattgg ataaagtcgg agtgataaat
     3721 acactgtaag gcacagcttt ccggttattc gttgttcgtt tattctcgcc cttcaactga
     3781 tccataatga ttcgataatc atctagagag atataagtga actgcaatcc gcctttgacg
     3841 tctccaatcg cccaaatgtt gtctgcatct gtacgaaggt attcatccac agcgattgct
     3901 ccgcgatctg ttagtttaac atctgtattt tctaatccaa gtgatgctgt acttggtttt
     3961 cttccagttg cagccaaaac ttttgacgct tggattgtta cttcttggcc atcttctgta
     4021 aaggtgacgg cagctgttga ttcttggtct gctacttgat ctacagatac acctagatgg
     4081 aattcgattc ccgcatttgt catgtcttct aaaatcattt gcgcgatatc ttcatcttca
     4141 cgtggaagaa attcagaatg ggcatcaaga acgacaactt tagatccgta ttcattgaac
     4201 attgatgcaa attccaagcc aatatatcca gccccgatga tcactaattt ttccggtaat
     4261 tgttcctgat ccatcgcttg cgtagaatca atcaagtagc gacttgtatt caatcctttg
     4321 attggtaaaa tgattggttc agaaccagta ttgatgaaaa tccgttcgcc ttcaacaatc
     4381 actttttcac cattattcgt cacttcgatt tgatggttag aaagaaaacg tgctgtaccg
     4441 tccatcactg tcccagttgc ctcatcgctg atcatatgat aatttttggc gcgaagcatg
     4501 cccgtcaatt tttctttccg agcaacagcc tctgtaaagt cgattcctct ttctccattg
     4561 aaaatcaatg atttagaagg aatacagccg atgttgatac atgttccgcc atacatacgt
     4621 gttgactttt caacgactaa aaccgattca ccttttgaag ctaacgcctt cgctaaagtc
     4681 ttaccgccct ttccaaaacc gatcacaata ttttgatatt tttccatcat ttttccctcg
     4741 cttctttttt acctaccgat tggtacaaaa atatgataac cgataattgt ctattctcca
     4801 aatcatctgc tttatttgtc aaaaaaaacc agacaaaaag cggtcctttc acgtttttag
     4861 tctggttcga tttcctttat tcttctaaac aatcgttact tctacatcga tatttcctct
     4921 agttgctttg gagtaagggc agaattcatg cgcatcttct gcaatttttt gggcttcttc
     4981 ttcgctgatg ccatcgatat gcacttctaa tactacaccg atctgatagc cgccatcatc
     5041 taaattataa agagagacac gtgcttttac tgtggactct gctgtcattc ctgcttcttt
     5101 gatcatatgt tgtaccgccc cattgaaaca agaagcataa cctgctgcaa ataattgttc
     5161 tggatttgtt ccttctgcat ggattcctgg aggagtgatt ttcatatgca tcgagccatt
     5221 tggtgcctcg acttctccat tgcgcccgcc atggttgatc attgctgttt cgtatgcttt
     5281 tttcattgta ctgctccttt cgattgacca tttgctactt ggctataatt atagtgtatg
     5341 acaagtaagg cgctttctac aaaaattctg cttattcgaa taccagaatg ccgtcttcta
     5401 ctatttttcc aaaaaacaaa aaaatactac tcgtgaacaa cagcgtcata tttgttccaa
     5461 aataaatagc aatcttttct acataatctt ttatatgatt gattttaaga tacatatata
     5521 ccagaaagtt gaccccataa accaatactg ttaaaaaaat aaccatattc gttaccattt
     5581 tttcctcctt ttgctggtct ccctaccagc actcgattta accataccac tttttttgcc
     5641 gtccaagcaa ctagcctttc aaaaagactg gtttattctg ctgttaatga ttcaagatca
     5701 gaaaaatgtc cgaataattt tgctaatttt actaacgcca acttgttctt tccgaattca
     5761 tcattttcta tctgtaatac tagtaacggc tcatgtaagc ttaaacggat caaaaaccag
     5821 ccgtttccgt atggctcttc tagatcatat cgaatacctt cttcattttc cgggtcgaag
     5881 ttcatccctt tttcttcttc caacagtcgc ttcatttctt ctaatatctt gtttccttgt
     5941 tcgcgtggtt catcgctgaa caattttaat cggatttctt gtacttctac gggctgtttc
     6001 aactctttga tttgttcatc taatgtgata ccttttcttt ttaattctgg caaaagcatc
     6061 aggatctttg cgatgacata tgcaccatca tccagaaaat agttttcctt gaatgcagcg
     6121 tgtccgcttg tttcgatggc tagctgtgta tctttcccaa gacggttaag ttctaatgct
     6181 ttattgatta catttcgata gccggaaata taacgaactt ggatgcctcc tttttcttca
     6241 atgaatcttt gtagatgaga agaagtaggg gaattcgtca cgattcttgt tcctggatgc
     6301 tcttttaata cgattgtact taatactgcg atcaaattat ttcgattgat cactgttcca
     6361 gaagcactga caactgcaga acgatccaca tctgtatcga aaatgatccc aagatctgct
     6421 tcatttgcta aaacagcctg cttgatactt tccatcgctt ctttattatc aggatttggg
     6481 atatgattag gaaatcggcc atctggttcc aaaaattggg aacctgttga atccgcacct
     6541 aagctattca atactttttc agcaaagaat ccaccagcac catttccggc atcaacaatg
     6601 attttccaac catttagtgg tttgctttct agtgttccaa tccccttttt aatttttgtg
     6661 actaaatctt gcgcataagg tgtcaaaagg tctgcttttt ctacgcttcc agtttgttcg
     6721 ctttttattt tttgctgctg tgataggata aaagcaatat cttctttttc tgctcctcct
     6781 tctgaggtaa agaattttat tccattataa taatagggaa gatggctagc agtcagcata
     6841 accgcagcat cgcacgagaa ctgttcaaat tgagtggaca taaacatagc aggcgtagta
     6901 gctaattcga aatcgatgac atgtatccct tgttctttca gactttggat caacgcctct
     6961 ttcagtgctg gtccgcttat cctactgtca tgaccaatcc caatcgtcca ttttttattt
     7021 ggatcggttg atttcttctt tttctttatc cagtttgcta gtccccagcc gatctttttt
     7081 accatgtctg tcgtcaagtt tacttcatat ccttcaactg gtaatgcgat tcctcgaata
     7141 tccgatccat tttgcagcgc tttcatatct ttcatataaa agtcctcctc atttattccc
     7201 tcattttttc cagcttttct gcaaatcgct gtgcttcttc ttctgagcgg caaataataa
     7261 tgaatgtatc tacaccggcc atcgttccga caacttcagg gtaagccact tcatctagcc
     7321 aattcgttac cacatcggca ccatttcgtt cagtcaatac gataaccatg aactgaacag
     7381 actggatacg caaaacctcg cgtctaattg ctgtacgcaa acgttcttca tttaaagaaa
     7441 cagatggctg gttaaataat gtatagcgta ctattttgtt ttcattttga ctttttacta
     7501 acttcatttc tcgaatgtct cgagaaatcg ttgcttgagt cacatcggcg cctgcttcat
     7561 gcagccgcgc aagcagctct tcctgtgtcc caataggttg ttctgtaatg atctggcgga
     7621 tcagtgcttg tcgctgtttc tttttcatat tagacacctc tcaatgttga ttatagcaaa
     7681 agataagtaa tatttcttca tattttggta ttttttactt ttattttttg ttttcattca
     7741 ggttgctaac ctttttctat tttaacacgc aaaatcgtct tcattttttc aggtgctgat
     7801 tttcttggat cgctgatgta aatctctttg tgttctttag aagctctttc atagccttct
     7861 tttgcgatga atgcttctaa tttctcaaac gttgctggtt cttcggcata gggcccgacg
     7921 tgtaagattt gggcacaaag accttcttcc atctttttga atactagttg ttctttcaag
     7981 tcttccggaa gtttgctaga acgctgaatg gcttcttgtg ctatcgcttc tgtaacaaag
     8041 tcaggctgtc ggatcatcaa ttgataagaa aaatgttctt ttttcatcac tggttcatgc
     8101 aaatactttt cttgcagtcc ccattgtcct tccagtggat agacagtata catctgataa
     8161 tcaggaatca gccaattttt tttaggcgac atgcgtattg catagctgac tgcatataga
     8221 caggctactc ttcgtttaaa gtcttcctga ttcgggtcgc cgatccctct gatgctaaag
     8281 aatggttttt taggaacctc tagtatttgc gggctttttt tcatactata gctcttgtct
     8341 ttacgccatt caattttttc catttttctt cctccgcgaa attccattta gttatttatt
     8401 atcatacctc tttttgagtg acttggctaa tcgtgttgac actatttttc agtttgtgct
     8461 agaataagtg aagtaaataa atcgtacaga taacacgttt cggcgtgtcg agagagaggc
     8521 ggtttctatt tttataatag aaatcactct ctttttttac gaaatttttt agaggtgatg
     8581 taaatgacaa aacaaaaaca gctgctttta gtacctgcag taattgcaac agggatgatg
     8641 tcttttgcgg gcgtattgat cgagacggcc atgaatgtaa ctttcccaac tcttatcaga
     8701 cagtttgatt taacgactgc acaagtccaa tgggtaacaa cgatttatct tttgatgatt
     8761 tcgatcattg ttccgctgtc tacttatttg aatcgcaatt ttagtctcaa aaaactcttt
     8821 ttagcttcaa atctgctttt cttggcaggt ataatgatcg atttcttctc tcctaccttc
     8881 atggtcctat tgtttggtcg attgctccaa ggtgcagcga caggtatcgc tcttccgttg
     8941 atgttccata ttatcttgac atttgccccc ctcaatcgac gaggcgcgat gatgggtgtc
     9001 ggtactttga caacggccat tgctccagca attggcccca cttatggagg cattatgact
     9061 tcacatttta catggaatca tatcttcctg tttcttgtgc ccgttttgat tctttctttg
     9121 attatcggct ggtttgctat tcccgagatg gcagtacaaa aaagtggtta tttggattgg
     9181 ataagcgttt taggaatttg tcttttgttt agtggaagtc taacattttt gagtacgttg
     9241 ggaacagttt ccagctggat tgctttaatc atcggcggta ttggctggtt ccttttttac
     9301 tatcgaagta aaaaagtgga gcatccactg atccgactgt ctattctttc aaataaaaca
     9361 ttccgcttgt ttttgattgg cttcttagtt tatcagtttt tattacttgg tgtatctttt
     9421 gtcttgccga atttccttca gatcgttcaa ggaagtagtc cttttgtcgc tggattagcg
     9481 atgcttcccg gcgcagcggt tggtgctttg ctttctcctt tttccgggaa aatgctggat
     9541 caatatggtc cgaagaagcc aatttttgct gggctgattc tttctttgat tggttggcta
     9601 gcattattcc tactcatagg acacgcttca gtgggtgtat tgattgcttg tcatgtgttt
     9661 tatatgatcg gtatcggatt atcttacagc aatatgatga ccaccggaat gaatgcactg
     9721 aatgaagaac tacaaggaga cgggaacgca atgttcaata ccttgcagca gttttctggc
     9781 gctgtggcta cttcattagt agcggtcatt atcaattacg tccagcatca tacttcccat
     9841 aactatgaag tgtctactac attaggatca aaagcagcat taggcgtctt gttgctgttg
     9901 cttctcgtaa gctttgctcg attcgcctat tatctgttct ttgctaaaaa agcgtagatt
     9961 ccatatacgg caggtgttgc atctgccgtt tttttgtgtg aaaatggttg tttgtaaaag
    10021 gaggcttttt tatggaaaaa attgccatta tcggtggcgg gatcattggt atgactctag
    10081 ccagttactt agatacatca aaattcaatg ttgctttatt tgatgaaggg acaggacagg
    10141 caactaaagc tagcgctggg attatctccc cttggttgtc gaaacgcaga aacaaacaat
    10201 ggtatcgtct ggctaaagac ggcgctgctt ttttccctaa acttataaaa gatttccaac
    10261 ttgatcaaac gatttacgca caatcaggga cacttttgct tcgacctgaa aaagatttag
    10321 ctgaattggc tgttcttgcc gagaaacgaa aagaagaagc gccagaaatt ggaaacatcg
    10381 aatggctttc acctgaacag acatctcaag cacttccgtt attgaaacca gcagtttcac
    10441 tgcgtatcag tggtggtggt cgtctggatg gaaaagcata tttagaacat cttggtcagc
    10501 gtttagcaca aaaacaaatg gagcagatcc aggcaaaagt cactttgcat aagaagagcg
    10561 attcttggct catcaaggga ccgtttggag aatacacagc tgatcgtgta attttgacac
    10621 ctggcccggc acttaagcct ttattacaag aattaggcta tgctgcagat atacgtccgc
    10681 aaaaagggca gctcttagca tttcagacat cttataagat gagtcaagac tggcctgttg
    10741 ctatgcttga cggagaagct gatttcattc ctttcatgga tggaaagatc cttttaggtg
    10801 ctacccatga aaacgatcaa ggctgggatt tggctcctac tagcgaagct tataagggat
    10861 tgactaagag tacagaacag ttcttgactg atccagatca gataaaaaac tggccatacg
    10921 agttccgtgt aggtacccgt gcttatacgt cagatttttc cccatttttc ggcgtactgc
    10981 cagatgatcc aagcttactg gctgctagcg gtctaggctc ttctggcttg accacaggtc
    11041 cttatatcgg ctatctatta gccaacttct taaatactgg tcattggtct acggaagatt
    11101 atcaaaagga catctccacc tatattacga aaaatccatc atagaaaaac agctttttca
    11161 agtaaggatc ccgagcgata ttgcgcatgg attacttctt gaaaaagctg ttattttagt
    11221 tcaatttctg aatcaaattc tccaaacttt cttttgcttc atctggcaag agatcttttc
    11281 ctatctgagc ttcaatgaat cgtaaacgaa tatcaagtcg ttgtttcacc agatttttat
    11341 aggacgtatc ctcaaaagaa acaggaaaat ctggtatagg taaatagtgg atgtcttcaa
    11401 atggaccaaa cgacttgaat tctgccgttt gttgcacgat tctttcaacg attgacaatg
    11461 tcgtttcagg tttgattttt ttatcatcga aatatccagt attcaggaca tagcaggctg
    11521 tttgctgttt caaaaacaac tctcgaaaag cttgatagtc ttccctaaga ggatagacac
    11581 ggaaaggatt agcaaatggt tcaatgacta gctcatctag ctgtcttttt gtagtgatat
    11641 tttccgcgtc tgatcgtttt gttgctaatg ttgcaccaaa agacgctgct aaaactgggt
    11701 cattgatctt gataattggc ggaagcgaat catctttcat gatccaaaaa acagcatcga
    11761 tcttttcgct aagatgatcg actcggtcgg gtgtcgcata acgtgatttg actgtccgcc
    11821 catttccgtt tcgtatatct cctgttacta ggactttttc tcctttactt gtacatgtca
    11881 cgcccacatt ctgacaagta aggaaagaag aaatggccgg atcatcagac ggatagtctt
    11941 gtgttttatc aaagtaagaa ggttccaagg cagtagcaaa accatttttt cgagaaataa
    12001 cgaaagcatc gtcatgcaag atcgaaacag agtgaccttc cgtatgtttt gctaaggtaa
    12061 tcgttgattt tccagaaccg gacaacccaa atacagccat cgtataggtc tggtcattac
    12121 tagcatattg tttcatccca ccgtggcagg caataaagcc attgcgattt cccaatgccc
    12181 aagcaagcgt caaagtcgct ttttttaatt ctccaaagta acgtaatcct agcacagccg
    12241 ctgtattatg gagagcatca aacaaaatga gtccatttgg aaatgtttca tgtgtccatt
    12301 cgggatcaca aaagatataa agatctccct catcaaagga aacagacttt tgataagtct
    12361 ctaagatctt cgaattcgct ggctgaaaat tcagcaaata agaatacaaa ttgttctcaa
    12421 atccttctgg aatcagcaaa tgactttgca cagaaaattc ttcagatagg ccaacgatta
    12481 cttttgtatg gtaaaactgg cgcttgcgac tttgatacac agcttctctt aaaattttct
    12541 cgtatttgct ttgttccttg tctaagtcct caaagatccg ccgagcactt gctgttcttc
    12601 ccacaatgct tccatcattg aatactagtg tcctaacatc agctggtaaa tctagttctt
    12661 cagcatgtga gattgttaaa tctgtgatga tcgttcctgg actccctttt gctagttgat
    12721 aagctgtctt cctgtcaaaa acttcatgaa cattattacc gtaaaatgtc gtttctacaa
    12781 tcgatcgaat actggataac agtggatttc cacttgtgat ttcgtttaat tcgagactct
    12841 cttttgtact catggctcct cctcattccc taaacaaaaa aacgttttct ttattataca
    12901 ttattcgagt ttttatttgt actgctgcgc tgtgggaagc aaacgagtta tttttcacac
    12961 aaaaaaaccg agaacttgaa taaacaagtt tctcggtttc atcattttcc ttttatcggc
    13021 ttagtaccag ccgtttgcta accagaatgt tttagcagca tcccatgaac cataacgaga
    13081 tgttacatat tgttctgcta cacgttcttg attggcagct gaatagtcgc cgtttaagta
    13141 tgaagcatct aattgataac ggccgatgta acgtccgtta gtagctgtgt atgaaccgcc
    13201 tgattctttt tgtgcgatcc attcttttgc agagcttgaa gttggcgctg ttgcagcagc
    13261 tggggcagca ggagctactt caactgtttc aacaggtgca acgtatgtat gttcttgcgt
    13321 tgctgtttca gttgtagatg ctgtattttc tgttgttttg ttttctgttg ttgctgaaga
    13381 atcatttgaa gttgggattg ttaattgttc accaacataa atgcggttga tgtttgcgat
    13441 cgaatttttt tcagcaattg catcgatcat tgagttgtct cctgcaaatt tttgtgagat
    13501 cttagataat gaatcacctg attttactgt ataaacttca tcagcgtgag catttgtagt
    13561 tcctacaaac attgcagtag caccagcagc aaaagttgta cctaaaacaa ttttcttaaa
    13621 tgaattcata ttaaactcct ttttttctcc gttttttatt ctttcgacgc tattaacttt
    13681 atcatttaga aatatttcat aggtaaaaga aacatgacat ttaatgacaa aataacgaat
    13741 tatttaatat ttgtaacatt taaacaaata tttttttgat acaaaaccat aaaagccttg
    13801 ttgtataag
//
LOCUS       DAJSBL010000067        13775 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.069, whole genome shotgun sequence.
ACCESSION   DAJSBL010000067 DAJSBL010000000
VERSION     DAJSBL010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13775
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.069"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            99..293
                     /locus_tag="ONT85_002383"
     CDS             99..293
                     /locus_tag="ONT85_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2379335.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
     gene            562..861
                     /locus_tag="ONT85_002384"
     CDS             562..861
                     /locus_tag="ONT85_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2379336.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(958..1392)
                     /locus_tag="ONT85_002385"
     CDS             complement(958..1392)
                     /locus_tag="ONT85_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR2379337.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1624..2334)
                     /locus_tag="ONT85_002386"
     CDS             complement(1624..2334)
                     /locus_tag="ONT85_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HCR2379338.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(2324..3184)
                     /locus_tag="ONT85_002387"
     CDS             complement(2324..3184)
                     /locus_tag="ONT85_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HCR2379339.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(3189..3827)
                     /locus_tag="ONT85_002388"
     CDS             complement(3189..3827)
                     /locus_tag="ONT85_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HCR2379340.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3842..4141)
                     /locus_tag="ONT85_002389"
     CDS             complement(3842..4141)
                     /locus_tag="ONT85_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCR2379341.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(4184..5668)
                     /locus_tag="ONT85_002390"
     CDS             complement(4184..5668)
                     /locus_tag="ONT85_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HCR2379342.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5688..6149)
                     /locus_tag="ONT85_002391"
     CDS             complement(5688..6149)
                     /locus_tag="ONT85_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCR2379343.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(6167..7234)
                     /gene="ulaG"
                     /locus_tag="ONT85_002392"
     CDS             complement(6167..7234)
                     /gene="ulaG"
                     /locus_tag="ONT85_002392"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HCR2379344.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            7452..8222
                     /locus_tag="ONT85_002393"
     CDS             7452..8222
                     /locus_tag="ONT85_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR2379345.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(8495..9322)
                     /locus_tag="ONT85_002394"
     CDS             complement(8495..9322)
                     /locus_tag="ONT85_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR2379346.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
     gene            9890..11125
                     /locus_tag="ONT85_002395"
     CDS             9890..11125
                     /locus_tag="ONT85_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HCR2379347.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
     gene            complement(12124..12384)
                     /locus_tag="ONT85_002396"
     CDS             complement(12124..12384)
                     /locus_tag="ONT85_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HCR2379348.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(12411..13712)
                     /locus_tag="ONT85_002397"
     CDS             complement(12411..13712)
                     /locus_tag="ONT85_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCR2379349.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
BASE COUNT         4425 a         2825 c         2173 g         4352 t
ORIGIN      
        1 tgattctttt tatttatcat aaagatatag atacataatc attttcactt acatatattt
       61 gaatattaac taaaaacagc aagaaagaag aggatcaaat gtattatgta gaagtaaaaa
      121 caaaaggcgt caaaaacaaa caatatgtga aaggtatcag taacgaatat cctttacttg
      181 gttcatggaa agaagctgct cctttttcca aaccatgtgc cataaaaata aagaacgagc
      241 tagaaaaaga actgacttgt ggtaaagctg ttgtagatat catcgaaaag taaatccaat
      301 gtatagaaag gaagatcaca catgaaaagc gacgtacaaa ttgctaagat tatagaatta
      361 gaagaagaag acgcagtgtt gacaattgaa aaaaaataaa tgtttgtata ccatatgatt
      421 atttcaatat gtatcctgga attggagatc tcgtagagct atatcaagat gaatcaaata
      481 tacttgtcat tcatcatgaa atatagagac ttataaaatt gtttattttt ataatgtatc
      541 agtatagaaa ggaaataaaa catggattat actgttgaat ttgacagtga actacttaag
      601 caatttccac aagaagaaca tttcatgtat aaagaagcaa tttcccacaa agtgacagag
      661 tatttggagc ataatttttt tcgtataaag ccagtgcgta ctcgttcggc aaaaggaata
      721 tacgagatga aagtcaaaat taataaaaaa aattatcgca tagcatttgc tttaaaaaaa
      781 aatcatgtgt tcatttttta tattagttcg aatttgcaaa aaattgtatt tgataaagag
      841 gtgagtaaaa aaataaaata gctgagcatt ggaggataga ttgagtgata ctaagtctat
      901 cctcttgttt ttctaaagaa ctaagctaga attttttctt ttgtacgtta ttatatgtta
      961 tttgtcatta ccaaaaagca ccttttttct tctagtatct atgacataag aaataccacc
     1021 catttccttt acttgaacaa atcctactga ttcataaaat ttttttgttt tctcagtatc
     1081 atctgtcaat aattcgattt ggactggtcc ataactagtg atcttctcca aagcttcatt
     1141 caaaagcact ttgcctattc cttggcgctg atacctcgga tgaatgagga tatcttgaat
     1201 aaatgcgatc gtcgcaaaat cagtgatcca acgaacgatt ccgataatct tcccttcccc
     1261 attacgcttt atcagagatt cagattgttc aaatgctcta gctaaccttg cagtgtcttt
     1321 tgtgtaattc gtccaaccaa cgctcttata taattcagaa atctcatcga tcataaggga
     1381 atcaatattc atttttttct tcctttctta tacaattctc cctctaataa tacagttctt
     1441 tgactattta atctatcaca tataactctc ctgtaaagat tctctcgatt ccttaaaatt
     1501 cctctttcct tttagaatgc atactttttg ataaataaaa aggttgtgat aaattttctt
     1561 cacaacctct gtttctctct atcaagtgtt atcttttaat tttctaccct taccctagca
     1621 tctctattgc ccgtagtatg catactttcc atgtttgcga aaataatgtt tatctagtaa
     1681 atattgcggg atttcacata tattggggtt gatcagctcg ttttctttgg ccatcagaca
     1741 aatttcatct aagatcaaac tgttttctac tgctttcata ggtgaatccc cccatgtgaa
     1801 cggtccatgt ccatagacaa gtactccagg gacttcgttt ggatctaatt ttctttcctt
     1861 aaacgtctcg acaatcacat tccctgtatg aacttcgtaa gcttctctta cttcttcttt
     1921 cgttaattgg cgtgtacaag gaaccttacc ataaaaagca tcagcatgag tcgtgccata
     1981 agctggcaaa tcgcgtcctg cttgcgccca catgacggag tgcgtggaat gagtatgagt
     2041 aatagcagtt atatcttcaa aagtttgata caaaacaaca tgtgtgggga gatctgaaga
     2101 tggttttaag ctatcttctt ctattatatt tcccgacaaa tctgttacca ccatttgatc
     2161 tgcttgcatg cattcatatc tgacaccgct tggcttaatg acgatgactc ctagctcacg
     2221 gtccacttca ctcacattgc cccaagtcaa tttgactaat ccgtatcgcg gcaatgccag
     2281 atttgcttca aggacacgtt gcttcattgc tgcaattttt tcttcacaag acatcatagc
     2341 cagcctcctt caatttagaa cttaagtagt cttttgcctg ctggatttct cttcgaaagt
     2401 cttggctttt ttcactccac atttcaatca gaaatgttcc atcataatct agacgtttca
     2461 aatgtcttaa taatccaaga aaatcaacac aaccgctacc aaaaggaaca tcacgaaact
     2521 ggcctgaata ttcttttgtt acagggtaag tatcttttaa gtggatagca gtgatataat
     2581 cgatcccttt ctcaagttct cgagccggat tattttccgg ccatgcggac aaattgccta
     2641 ggtctggata tacttgtaga aaaggtgaat gaatctgttc tttgatttct aaaaattttg
     2701 aaatggagtt cataaatgga tcatccatga tttccacgga aagaatgatg ccatatttag
     2761 aagcttcctt tacgctttct tttattcctt cgataaagtc ttctctggaa ttcattgatt
     2821 tctcttcata ataaacatcg tatccagcta tttggattac tttgacgctt agattatgcg
     2881 ccaaatggat tgcttttttc attagttgtt tcgctgcttt tttcttttct ggatcagcag
     2941 aaccaaacgg aaatcgccga tgcgcactta aacaaatcga atggatacgt acaccggagc
     3001 tgaacatctc ttctctcagc tcagctatct tttcttcaga ccaatctaat cgtgctaacc
     3061 gctcatccgt ctcatcaatc gacatctcga taaaatcgaa gtccatttct tttacaaggg
     3121 aaaaacgttc tttccatgaa atatctttgg gtaacgcttt ttcataaatc ccaatttgag
     3181 ccatttcctt acccccagat acgaatgatc tcatctttga agttttgtgc ggccttcatc
     3241 ggatcatttg ctgctgtaat accacgacca gtgataaagg tatacacatc gatcccttca
     3301 aataagtgta atgtgtgtgg atttaatcca ccagttacag aaacattaaa gcctagttca
     3361 atcagttttt tgatcttcga taaatctttt tcaccccaag tttcaccaga taacagcgca
     3421 tcacggcttt gatggtagat tacctgccgg ataccgatgt tatgccaatc catggcttgt
     3481 tcaaaagtcc aattgccgta gagttctact tgcaattcac ctatttcttt ttgtgctgcc
     3541 tccatagttg gaatcgttgc acaacaaatg actgtcatcc agtctgctcc tgcttttgca
     3601 acattactcg ccactgttcc tccagcgtct gcacatttcg tatcagcgac tagtttttta
     3661 tctgggaaca tactgcgaat acagcgaatc gctttttgtc cttcctgtaa gcaaagaatt
     3721 gtgcccactt caataatatc tactacatca ccaactttca taacatctcc taatgcatgt
     3781 tctaagtcat tgtgatctaa cgcaatttgc aaattcggcc tgctcatctt agttccttcc
     3841 tttacttgtt tttttggtac cactttgttt cagcaaattt ttcactgatt tctttgactg
     3901 acatcacatt tttgataccg ataactgtaa ctcctttttt ctctgcctga gtaaacatat
     3961 tcaaaaaatt gactggcgta aaaactacat cgtattggcc agctgaactt tttccctctg
     4021 aaatggcaca atgatggatt ttggtaattg gtacgtttag ttctttcatt gctttttcca
     4081 cacttctcat catcatcaag cttgttcctg atccatttgc acacgatact aaaattctca
     4141 tcttaatccc tccattatga gtgttgtcta atcagatttt cttttatact ctctgctttt
     4201 ctgcatatgt gtcatagtct tctacaatca aaaaataacc atcaggattt ttccgatatt
     4261 gtaactgcgg aatagccaat aaaccaatga caactacggc aattccagca taacctagga
     4321 ctttcatgat tactgtaaac aatggccaag ctgttgccca gtcgaacatc ccgatataac
     4381 caccgtatgt tgacatccca atccatgtag cgaataatgc tgaaccgcct acttgtatga
     4441 ttcctgaaag aaacgggaac aggcaagcgg ctttgaatcc acctcggtta ttggcataaa
     4501 ctgctataac ggcattatca aagaacaacg gaatgaaacc ggctatgacg atcgttggtg
     4561 atttcaataa aatcagtaaa ccaatcatca aaaactgtcc caaagcacca aacaagaacc
     4621 caatcgttac cgcatttggc gaaccaaagc caaaagtagc agctacgtca attcctggaa
     4681 cagctcctgg taaaatcgtg tttgagattc cttggaaaga ttgagttagt tcatcgacga
     4741 atgtccgaac acctaattgc aagatcgcca gatataccgc aaaatttaaa gaagtcgtca
     4801 taatgtagaa taagaaactc tgcccttctt gcatgaattg cgcttgaatc aaataatctt
     4861 tgcctagtac aatcagaatg atcccaaaga aaaatagcat gagtaacgaa gtagcaacca
     4921 tattctcatt gaaaatcgac aagaatcctg gtaatttgat atcttcaatt tttttgtctt
     4981 gcttgattga tctatttttc ttttcatccc gctttttcat ccactcagct aattttgcaa
     5041 atataaagat tccgaacatt tgctgatgtg ccactgcaaa tccggcacct tctgtcaatt
     5101 cctgcgtgat tcctactgtt aagttagaag ctactgtcca ataacaacct aaaatcagtc
     5161 ccataaaaat caatgcttgt atcctaccta gatcaggaaa gcaaaacagt aaaatccaaa
     5221 aagcggtagc tgcttgttga acctgaacgt ttccggtagt gaaaacggaa cgcagtttcg
     5281 tatatttctg aaaacggact aataagatat ttaaaacaaa tgcaatcagt aataaaatca
     5341 tcacatctcc aaaggtacgg ccaaatgtct ccattaatcc agcatccaca gcattttgtc
     5401 caaaataagg atctgtaacc atggcatcca aattgaaccg ttcctttagt ccaaccaaga
     5461 ttggccgaaa attattgacc aaaccaccag agccaacagt caaaataaag taaccgaccg
     5521 ttgcctttaa gaatccagcg atgcattcat gaagcgggcg tttcagcaaa atatagccta
     5581 ataacacgat aaaaccaatc aggtatgctg gctgcgtcaa gatatttgtt gcaaaatacg
     5641 tccaaatgct taacaaaaat tcccccatct tttttctcct ctatcaatta attatattct
     5701 gccatcacaa cttggtaatc atcgatggag ttcgtagaga tgagacgctc aaccaatcca
     5761 tctgtcatta ataattcgga taatttttgg atattttcta gatgttcttc tggatctttt
     5821 gctgcgagtg taaaaaacaa actagccgtt ttctcctcat tctccccttc actttcaaaa
     5881 cggattggct gtttcaactt ggtaaatgaa atagctgttc caaaaacacc atcacttttt
     5941 tctgaagaat gcggcattgc tacttgaggt gcgatcacaa tataaggacc gtatttttct
     6001 acacaatgga tgatctcctc cacatacgtt tcatcaataa tttccttatc gatcaataca
     6061 agacagcttt cggcgatagc ttcacgccaa tccgtgattt ctttatctgg gtaatgaatc
     6121 aattgatggt caaaaaagta tttcagcaag aaactcccct tctttcttat aaaaacgacg
     6181 gaaacggtgt atcattttca atcgagaaga catcttcaaa tcctcgataa aaattaaact
     6241 caagatcatc tttgtttgtc ggatagatga atttcccgcc tacttgccag atataaggtt
     6301 taaatccata cttcaggcga tcctttttga atttccaaat ttcagtgatc tcttttggat
     6361 cagccataaa attagaccaa atatcgtagt ggacaggaat cactactttt gcattcaatg
     6421 actccgccat gcgcagcata tcaacagaag taactttgtc tgttatccct cgtgggtttt
     6481 caccataggc ccctaaacag acatcgattt tgtgttcatt accatgtttg gcaaataaat
     6541 tggagtaatg tgaatctcct gcatgataaa gattccctcc tgatgtttcg aataaatagt
     6601 ttactgcaat ttgatccatc tcctgaggca tttttccttt taatacctct tctggatctt
     6661 cacaagtgac caacgctgtc cgatcaaacg cttctaaagc tactacattg atgtctttga
     6721 ttttgactgc atcacctggc tttactgtaa tcgttttttc ttcaggaatt ccccattttt
     6781 gccacgtgcg aaccacttct tttggcccaa tgaatgttgc ttcaggacaa ttttgatgaa
     6841 ctgctgcggc tgtatggata tctagatgat cggaatggat atgtgtaacg attaaagcgt
     6901 ctacattttt tatcgcaaaa ggatcgatta cgaaaggctg agtacgtaaa ttaggctgca
     6961 tattttgaac gccactcata cgcatcatct gatggccttt tttcatcatg ccattcccat
     7021 gactacgttt tcccgtccca caccatagat cacataaaat attcgttcct tcatgacttt
     7081 tcagccaaat tcccgtacag cccagccacc acatagcaaa agttccagga tccactactt
     7141 ctgtttcgat ttcttcattc aagtatgtgc cccactcagg aaatgtttct actaaccact
     7201 tttcttttgt tacttcattg acagttttac tcatttgctt gctcccccca tatcgttgtt
     7261 ccaacttaca agtaaatatt aatgtgtatc agcatccatg aaaacccttt tatatgaccg
     7321 tttgtttttt atatgatttt ctttcagtaa tcaatgattg tttcagtata ataaagacag
     7381 atatgaatgt ttaaatgaac aactagtcaa ataaatccat ggatcaagat gtccctcagg
     7441 aggcaaaagt catgaaaaac tcacttgtca atattgaaaa acgtcaaaaa gaaattttgg
     7501 aactgcttca gcagactgaa caactatcaa ctttcgaatt agctgaatcc cttgatgtat
     7561 ctattagtac gatacgaaga gatttaaatt tattagaaga aaaaaacgag atcatacgca
     7621 attacggtta ttgttcttat aattacaaaa accagactga ttttgatcaa tctggtcctg
     7681 aaaggattaa acaagcgatt gccagagaag ctagtaaata cattaacgat tatgatacgc
     7741 tgtttatcaa ttctagctcg actgcgttga aaacactgga ttttcttcaa tctaaccatt
     7801 tgacagtcat tacaaacaac gtgaaaatca attactctcc gcacaaagat aattgtagtt
     7861 atattttaac tggtggagag atgcgctttc caaaagaagc actagtgggc gacatcgcat
     7921 taaatacgat ttcttcagtc aatgcagaca tttgtatcat tggctgtagc ggagtcgatg
     7981 tagtacatgg ggtcacaaca aagatattaa atgaatcaac gatcaacgaa atgatggtca
     8041 acaagacaat caaatgtaag attttagtcg ctgatcatcg aaaaatcgga ctgacatcaa
     8101 agttcaagat tgctgatatt tcagtctttg attatttgat cactgataaa ttttgttctg
     8161 aaaaagtatt agaggagatc agaaatacgg ggataaaaac cattcaagta gattcatttt
     8221 aagaaccagg acgtaaatat gtaaatagga ttatcaaaaa aatgaataac aaatttatcg
     8281 ataaatgaaa aacacgctac gctcaaattg ataaaggaat cccaagatgt atccatccaa
     8341 tcgataccat cataatctat tgcttctttt ataaccgtta tatgttgcct aaataggaat
     8401 caaacaatat cgaaaaaagg aggtatgaca aaagtcacac ctcctttttt cgaataaacg
     8461 gtattcaaat cagaacactg atttcacaac cactttattt caggtcgttt tcaatcacct
     8521 ttaccgcgta atcatttccg ttgaaacgcg ctattttttt tgcgtccata tatctttcta
     8581 aataatcatc ttctcctaat tgcttatggc aagaagcaat ttcataaagc aagtattcca
     8641 atgcataagt cgatttatgt ttcttattat actcaatacc cttctcacat aattggattg
     8701 ctttatcata atcctcgatt ttacggtaat aggtggcgat atcataatat acaatgcaga
     8761 ccatcagatt atcaaaatca gtctcactca atattttaag tgtacgctcc acataaactt
     8821 tcgcaaaatc taattctcct tttgacaagt agatcattgc cacgtttgca gctgcttgta
     8881 agtgatggat atctgtttcg gtcgtttgtt ccaacacttg attaaaataa aataatgctg
     8941 catctggttt tttcgcataa agatgatcaa gcagtccttg ataaaataaa atttgattta
     9001 aaagatcaga attacttact ttttcctgat cgatcgtttg taaaatttta taagattctt
     9061 ggtattttga tacagaattc aatgattgaa tctcatttat ttttttcctg attttgtctt
     9121 cttctgtttc tactaatata tcggagatct cgacattcag ctttttgcaa agcgcactta
     9181 agatattgat gcctggcgac atattcttgt tttcgatatt gctgacagtt gcttgttcac
     9241 aaatgccttc tgctaattgt acttgtgtta accccttttt ctttcggtat tcttttagta
     9301 atgctccatc tactttaatc aaaacaattt ccttcccttt aaaaaattat ttctccttta
     9361 tatcatacac aaaaaaaagt tctaaacaac caaaaatgtt tagaacagaa taaaaatttt
     9421 aattttaaaa taatattcaa attaaacaac tggcgtatct gctccttgat ttctttttgt
     9481 gttatctgaa taattatcgt ttacagttat aacacatagt gaaaattgtc atggcgagaa
     9541 agtctaagaa tatttttaaa cataactatt tctttcttac gtgtgtataa tgacgacagc
     9601 acacctatca attaatataa ttattttatt tttttatgtt ctcatgttta tttttaatga
     9661 cacaacttct cgtatttatc tggcacaaca tataaatcga tcaaaaaatt gttgcgtgtt
     9721 tttttgaaaa ttggagtttc aacttgatta caaaatttaa atttagaagt tgtgaaaaca
     9781 gcgagagaca gtttaatatt caaaatatac ataggctact cgaacgctgt gttttttagt
     9841 atactagtaa gaaacgaagg gatagactca ttccaataag gaggatttca tgaagacaat
     9901 tctttttgct cctgcagtat tcaatttagc tgagacaaca cgaatgattg aggttgccaa
     9961 agctatgaaa gaccgttatt actgtgtatt ctttggcttt tctaaaactt ttgctccttt
    10021 gatcacagaa agtggctttg agtatcattt actagcgccg atccttacga aaaaacaaga
    10081 agaacaaatc ataaaatttg accagttgcg tacacttaaa aaccctttta ccaaagaaat
    10141 ggttgcctct cgtatcacta gtgaacagag tctgattgct aagtttgagc ctcgactgat
    10201 tgtgacaggt tcgaatgtga caattttttt atcggcccgc aataaaaaga ttcctttagt
    10261 atatgttaaa ccctatgctt tgagtgaacc tttttttcaa tcagagcatg cgctgatgcc
    10321 ccacgagtta cgtttcttcg gccctttaag caatcccctt tggcactgga caaaaaagcg
    10381 tatagtaaat acccgatgga tccccaatgc cttcaaacac gctgcacaaa aagaaatgct
    10441 tcctttacca aaaacaagtt tacaaatgat ggatgctgat ctaaatctca tcacaacgcc
    10501 taaactattt ttggaagaag aacagctgcc ggaaaattat cgaatcgttg gtccaatttt
    10561 tgctaagctc agtacttcct tgcctgaaga agtgctaact tttatacagc gaaagcaaca
    10621 agaaagaaaa cgtattatct atttcgcaat gggtagttca ggcaatccgc gtatgttacg
    10681 caaaattttg ttcttcttac aaaaacaacc ggatctagcg gttattgcac ctattgacta
    10741 tctgcttgat gacattcaat ctgactcaga agatctttta ataacagaat atctgtctag
    10801 tcatcttttg catgaattca ttgatttttc ttttatccac ggcggggaag ggaccgtcca
    10861 aactgcctgt gaatccggca aaccgtttat cggtgtgggg atgcaatttg agcaaagctg
    10921 taatattgac tattgtgtaa aatacggaaa cgcaatcgcc ctcacaaaac cagtgactga
    10981 aagaaaactg cagattgctc tgcgcgaagt ttcttcaccc aagattcgtc agcaggcacg
    11041 cacgctgcaa gccgtttttc caataaatgg tccacaacaa gcaattcaag aaatcgaaac
    11101 cttcctgagc aaccacccaa tatagtttca agtagaaaaa gcttttttaa tagagactca
    11161 tcagtaattt cgctattcga gacccagaca gtgaaaatac aaaaaaagta aatagtggat
    11221 tgaaaagtta gagttactta aattacggtg tctaaaaaac acaaaatcat ccttaaaata
    11281 aaagatctga cgctatccaa atgagcatag gtagcgtctg ccatttattc ttagtcaaat
    11341 tctttgacac ctttctctgg ttggatcatc tcaacgtttc ttcaaaagaa agaccataat
    11401 aattaggcaa atacattttc ttaaaatgtg tcatatagat ctagaagcat cgctttgtag
    11461 agatttgtgc cgctagtccc ctcaacaatt actaaatcaa atgtaggatt taccacttta
    11521 tctttgataa tcaacctccc tcttctgttg agacaatgat ctcgcaatcc gatgcatact
    11581 cctatataaa ctgccagcaa tccccacata ttgagtagat catgtcagaa tgttctatgt
    11641 cgtcagcaac gatacactaa aagtcacgtt caccatgact gattgctatt cccagcacaa
    11701 ttatttctat acacgtttca tgatttgaat aaacactcac atcatacata ttaccattca
    11761 tacaccatac tgcagcccct actatatggt tgtaagctcc caaatctata tttgacttga
    11821 tcactttggt agtagatcta ctaattctaa atccattgct gataaaattt ccattttttc
    11881 ctccttcttt ctgccaaaac agttaaaaca ataataatct ttatgtaaaa aagggctgtg
    11941 acaaaagcct tgtcacagtc ccatattccg aataaacggt ggtcaaaagc ttgcatctgc
    12001 ggtaataagt tcaaccaaac gaaaaataag ccaacatttc acaaaaattt aagaagcaat
    12061 tttcgtaaat ttcttcttat ttcttgaagc taaccacttc tgttacaacc acttttagaa
    12121 catttaaagc ttcgatgtga caaaccatct atgctgctcg atcataaaat tgatagcaaa
    12181 aattccacaa agtacatatg tcccccatga aaacaaaaca ttcatatgcg gtacaatgat
    12241 ctttggaaga atcacgatca aaaatgatgc cagtgcccaa atccatcgat tgatttgaat
    12301 atgatatgta gctagcatat tcaatataag gtgactagcc gttaataaaa agataaaaac
    12361 aaaaaataaa atcagccaat acattgtttc ccaccttcta aaaaccaaac ttagacagtc
    12421 tggacttcgt tttctccacg actttgttca tctgacagca tctttttatc ataagctttt
    12481 acaaatggat aatagataat aaatgctatc aatgtgttga tcatgatcaa gataacaaca
    12541 cgccagtcat ttcctgctga aagataaccg ccgattggag ctggtaatgt ccatgttggc
    12601 aaaacagtaa atctattgac catttctaat ttgactgcaa aataactgat aatagtcaaa
    12661 actaaaggaa ctaagttgaa cgggatgaac atgtaaggat tcaacatcat aggagctcca
    12721 aaaattatgg gctcattgat attgaagata ccaggaataa tagaaaaacg accaacctct
    12781 ttcaagaatt ttgatttaca gaagaatata aacaaaacag ataccacgat cgttgctcct
    12841 gcaccaccaa ttgttaccgt ccattgataa aactgttcag gagcgatata gggtaattgg
    12901 gtggcagctg taccatctgc caatgccttt gcattctcat ccaacatgat cagccacatt
    12961 ggacgaacaa cagcccctac aatactcatg ccatggatac caaatgacca caacaaatgg
    13021 atcaaaaaca ttggtagtaa aacaccaagc aaattattcc ctaaaatatt ggttaaaggc
    13081 tcgaataacg ataataaggc agcgtttaca tcaaagcccg cgacgcttcg aatcagccaa
    13141 gtaattgtta aaatgactgc acctggaatc aatgcctcaa aactacgggc aacagcaggt
    13201 ggcacttgtt ctggcatttt gatagtaata tttcgatttt taaagaaacg atatacttcc
    13261 actgcaaaaa tcatagataa gatccccgca aacatcccag atgctcccaa agaactcata
    13321 ggtaagaccc agccaagagg tgcctcttca ggagttaaaa cgtttacagg aacttgtaac
    13381 ataaagaacg ttcctaaaga taatatacca cctgtcacac tatctaatcc atatgattta
    13441 gctagatccg aacccataac aaaagttgca taaatagcca tcaatcccat cgtcatccta
    13501 aagggtaaaa caatcgttgc cgaataaggg gcaataaatt cattccatcc aggaaacggt
    13561 aaattgataa agaccataaa aaatgtccca atcaaaatca atgacaatgt tgaaacaacc
    13621 ccattacgaa ttgctaataa atgacgctgt gtaccaattt ttgccattgg tgggacaagt
    13681 ttctcttcga cccatttcat cagcttttcc actaaaacca ctccttttca ataaaatatg
    13741 aaccattcat tgcatgacaa cgcaaagaat gttca
//
LOCUS       DAJSBL010000068        13731 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.070, whole genome shotgun sequence.
ACCESSION   DAJSBL010000068 DAJSBL010000000
VERSION     DAJSBL010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13731
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.070"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            1..1452
                     /locus_tag="ONT85_002398"
     CDS             1..1452
                     /locus_tag="ONT85_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HCR2379350.1"
                     /translation="MKKSLKLASAVILMSQLLIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSELLTTSSSESSTTSNAESSTTSSSESSTTSNAESSTTSSSESSTTSN
                     AESSTTSSSESSTISSTESSTTSSSTSEITSSKVTKPNHIESSKKEAPKTEGQTKTFS
                     TPQQIESTESFQSQPILSGNEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIA
                     QAILESASGQSQLAQAPNYNLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYEN
                     YEESFEDYAKLLTKGISGNKDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNEL
                     IKTYDLTNYDKEVKGPQMSKTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIH
                     AVKAGKVVKAEFHYSWGNYVVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGST
                     GNSTGPHLHLEICKDSSLSQSMLVDPKTLLFGD"
     gene            1801..2433
                     /locus_tag="ONT85_002399"
     CDS             1801..2433
                     /locus_tag="ONT85_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HCR2379351.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRCLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCHCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEK"
     gene            3021..4460
                     /locus_tag="ONT85_002400"
     CDS             3021..4460
                     /locus_tag="ONT85_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR2379352.1"
                     /translation="MYSYQEDAKVQKNRWWILIAISMFTFMSTLDGSIVNIALPTISD
                     DMGIPMNQAEWVVSVYLMIVCSCLLLFGKIGDSRGKIKVFRIGTLIFVLGSLLCGFNH
                     SLGILLFARVVQAIGASMTMATNAGIITEVFPIHERGRALGAIGAFVSLGSIAGPGLG
                     GLILSQFSWAYIFWINVPVGIVTMLIAEKFLPKDITMSGHTIDGGGFAAIAATIISFF
                     GAIFLGQESGFFHPVPLVLFVISVIAFVSFLKVEKKAASPLISFGIFKNKIFTMSLIT
                     ALLIFSSNFFVNVVIPFYLQNARGLSPSYAGLLMMVFPLMMVVGAPLSGYLTDRVGPE
                     ILVLVGLGLLSVTQVMYMFLSVDSPLWLYIVATAIMGAGNALFQSPNNTMVMSSVTIE
                     NLGLAGSMNSFARNLGMVSGISLSTTVLYQAMSAKMGQQVTNYIANRPDVFLYGMKIT
                     FLGSFVLCFVAFFLTIWRIKKQGGIQKNV"
     gene            4755..4973
                     /locus_tag="ONT85_002401"
                     /pseudo
     CDS             4755..4973
                     /locus_tag="ONT85_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251366.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            5222..5437
                     /locus_tag="ONT85_002402"
     CDS             5222..5437
                     /locus_tag="ONT85_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2379353.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(5654..5910)
                     /locus_tag="ONT85_002403"
                     /pseudo
     CDS             complement(5654..5910)
                     /locus_tag="ONT85_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(6097..7008)
                     /locus_tag="ONT85_002404"
     CDS             complement(6097..7008)
                     /locus_tag="ONT85_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HCR2379354.1"
                     /translation="MKIKIERNQKEKSPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGVESFTEDFAKEYFSWKNNKEAIEKRMSNL
                     EQYLTENGLALIQDMVRADIPTSSEVQSVKILDVEKHSEEFVVSFLVEQHITEGKKTQ
                     SISSAYRVTIFEDENRNHLVTSLPTMIGKPDKAKYEAKQVENDSEIDAKTTEEITDFL
                     ETFFKLYPSASEKELEYYVENGAMRPINIDLKFSELENPVYLQNGKNFLVSTKIKYLD
                     NVSKTTSYFQYKLVLQKNGNWKIVDVK"
     gene            complement(7027..8031)
                     /locus_tag="ONT85_002405"
     CDS             complement(7027..8031)
                     /locus_tag="ONT85_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HCR2379355.1"
                     /translation="MNLKKVGVISLTFLTISFMGILMCVGLLFGEDSEGNGSSGTSPG
                     GTSVSEEVLKHRAMVEKYAKESDITEYIPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNYGGGYLDHVGKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKIVQEIF
                     DEAFKYEGTAYVFGGSSPETGFDCSGLTQWCYAKVDLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTENYWRQHLIGAGRYK
                     "
     gene            complement(8028..10151)
                     /locus_tag="ONT85_002406"
     CDS             complement(8028..10151)
                     /locus_tag="ONT85_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HCR2379356.1"
                     /translation="MKKKLFRIVGIVFISILTLIVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALTLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRALAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEILSDLTKEN
                     PVSNNKKQSRMNLAGRKIGKVLDTQALVKDKTKQVKDQVRNTPTNLKYNLHKGIEKSK
                     KAPEEFKRGLFQEKADRVDLREKQRQRRNAKMDEKRKILSEATNRQGKRRANIPIDKA
                     AQPQKDMMLKKEAPKRIVRANLNPEIKRKLTSQEIISKGKSQSASKKNSFQQVKQRST
                     LPKQTNQKVQKRMNHRPKSRSGEKK"
     gene            complement(10156..12603)
                     /locus_tag="ONT85_002407"
     CDS             complement(10156..12603)
                     /locus_tag="ONT85_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCR2379357.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELIPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLTEVAKQRIDLQTEALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKNLKKSFFSGFQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVRRVTPSDLMYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPSRCLIEEKPRYLCMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKDSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEINILNLTSKPKNQGLLDPYVIMEKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSNQRGLLQVINELRKEGSPVAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDRQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDREDESNQKRLRDLENGQCLMQDLYGRVGVVQVHPVFE
                     ELFHAFDTRPPVQERME"
     gene            complement(12590..12979)
                     /locus_tag="ONT85_002408"
     CDS             complement(12590..12979)
                     /locus_tag="ONT85_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HCR2379358.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEGAFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            complement(13039..13542)
                     /locus_tag="ONT85_002409"
     CDS             complement(13039..13542)
                     /locus_tag="ONT85_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HCR2379359.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELEGTPVGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCDDMSDVAYFLIVEAGDFGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPY"
     gene            complement(13555..13716)
                     /locus_tag="ONT85_002410"
     CDS             complement(13555..13716)
                     /locus_tag="ONT85_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2379360.1"
                     /translation="MAIVVIGLFLGFKREFSKLIGFLVIALIAVGLVFNASGVKDVLL
                     SLFNRIVGA"
BASE COUNT         4221 a         2710 c         2509 g         4291 t
ORIGIN      
        1 atgaaaaaat ctttaaaact tgcatcagca gttatattaa tgtcccaact ccttatagca
       61 acacctttcc atgtgttggc aagcgaaaat tcaacataca aaattgcaaa agaaagaacc
      121 gagacgactg aaatgagtaa ttccgatgta acagcagaga gtagtgaact tttgacaact
      181 tcatcaagtg aaagtagtac gacttcaaat gccgagtctt caacaacttc atcaagtgaa
      241 agtagtacga cttcaaatgc cgagtcttca acaacttcat caagtgaaag tagtacgact
      301 tcaaatgccg agtcttcaac aacttcgtca agtgaaagta gtacgatttc aagtaccgaa
      361 tcttcaacaa cttcaagtag tacaagtgaa ataacttctt cgaaagtgac aaaaccaaac
      421 catatagaat cttcaaaaaa ggaagcgcca aaaacggaag gacaaacaaa aactttttcg
      481 acaccacagc aaatagaatc aacagaatct ttccaatcac aaccaatact atctggtaat
      541 gaagaaattc attttgacaa aaatgaatct gtagaaaatt ttattatgaa aattggtgag
      601 tctgcgagaa aaattggtca gaaatatgat ttatatgctt ctgttatgat tgcacaagcg
      661 atcttagaat ctgccagtgg tcaaagccag ttagctcagg caccgaatta caatttattt
      721 gggataaaag gaacttataa tggaaacttt gtcattatgg taacaaacga agatttgggt
      781 aatggaacgc tttatacgac ccaatcaaaa tttagagtgt atgaaaacta tgaggaaagc
      841 tttgaagatt atgccaaatt attaactaag ggaattagtg gaaataaaga tttttatgca
      901 ggagcattaa aagctaatag taaaacatat cgagaagcaa ctaaattcct tacaggacgg
      961 tatgcaacag atacacaata ctatttgaag ctaaatgaat taattaaaac ctacgattta
     1021 accaactatg ataaagaagt taaaggtcca caaatgagta agactggata ccaagtgcct
     1081 attcataatt attcaataac cagtttgttt ggaaatcgtg gaaatgaatt tcaccgagga
     1141 cttgatatgg cagctacaca gggtgagccg attcatgcag ttaaagctgg taaggttgtt
     1201 aaagcagagt ttcactactc ttggggaaat tacgtggtga tacaacacga agatggatca
     1261 accgcgttat atgctcacca gcaacaatac ctagtaaaaa ctggagataa tgttttgcaa
     1321 ggacaaataa ttggctatgt aggaagtact gggaatagta ctggcccaca ccttcatttg
     1381 gaaatttgca aagatagtag tctttctcag agcatgttag ttgatccgaa aaccttatta
     1441 ttcggagact aaaaaaggta atcaatctga caaagtaaca agaaaatggt tggattctta
     1501 gacctttgat caggatattg agactggaat tgatatctcg attacgatgg gtttgacaaa
     1561 taaaacaagt ccattatcga atatcgagtg tattattgcc gtctttatgc tcatattttg
     1621 agcaggtttc actagatgaa aacaatttat ctatcttgag gatttcgtgt acgtatcgtt
     1681 cagtcttgta ttgtaaatta gtcataaaat cagactaaga aaaattggaa atattttttt
     1741 ctgatttatt aaaagttaat tgaaattata tggtacttct ttcttgggag ggtaggtgca
     1801 gtgaaggaaa gatatggtca ccttactgtt gttgaggaga ttactcataa tggatataaa
     1861 aaatatcgtt gcctatgtga ctgtgggaat gaaacgattg tatatcgatc aaacttacta
     1921 tcaggtagaa ccaaaagttg tggatgtgga aattataaaa ataggcataa atataaaaat
     1981 ttgacaaata aaacttttgg taagttagtt gcaatcaagc caactaaact gcgaagtgaa
     2041 ggaactgttg tttgggaatg tcaatgtctg tgtgggagaa aaaagttagt atcggcaaag
     2101 cgacttctaa aaggtgatgt aaaaagctgt ggatgtttga aatcaaaaaa gcatcacatt
     2161 gaaaataaaa aattcggatt acttactgca aagtatcctt tggacaataa ctgttcatgg
     2221 aaaacaaaat gggtttgtca ttgtgagtgt ggcagtatgt gtgagataag ttatagtaac
     2281 ttattttttg gacatactca aagttgtgga tgtttaaaaa ggcaagaaac aaggacatgt
     2341 gttgaaggga cagtaataga gtgtctaacg tcaaaattac cgaaaaataa tacaagtggt
     2401 gttaagggag tttatcgttc taaagagaaa tagattgcgt atataacttt aaaaaaaaag
     2461 aggtactatt taggatcgta tgattctatt aagagtgcag ctcaagctag aaaagatgct
     2521 gaaaataaat tgtttcaacc tttgattgac aaatatcgag gtaatcatta attattgtgg
     2581 ataataatta acagtgatat ataaacatct ataatatatt attcatacta ttgatattta
     2641 gtataatcct atggaattga atatcatagc aatgtatttg aaaagcaaat tggaaaacaa
     2701 ataggtgcaa caccataaat atgaaaaaat tgggaaaaag tagaatggaa atttgcttac
     2761 gtctcacgag atagctcaca ctattaatct gaagaacaat gaagtcgact tttagaaatt
     2821 gctagaagaa agtaaagcga attatcacta gttactatag agtaagttca aataaagcat
     2881 ctccaactta catggttaca tgaggatatc aaatcgaaat tactcaagct tcctatggct
     2941 agagtcgcga gaagttgttg ggcagtagac ataggttata ttgttcttag ctacacgatc
     3001 attagaaagg aaatcctatt atgtatagtt atcaagaaga tgcaaaagtc caaaaaaatc
     3061 gctggtggat tttgattgct atatctatgt ttaccttcat gtccacattg gatggcagta
     3121 ttgtcaatat tgcactacct accatctcag atgatatggg cataccaatg aatcaagcgg
     3181 aatgggtagt gtccgtttat ctgatgattg tctgttcttg tttgttgctg tttggtaaaa
     3241 ttggcgatag ccgtgggaaa atcaaagtat ttcggattgg tacgctgatc tttgtcttgg
     3301 gatcgctgtt gtgtgggttc aatcattcgc taggtatttt attgttcgca cgggtggtac
     3361 aagccattgg tgctagtatg actatggcta caaatgccgg aatcatcacg gaagtctttc
     3421 ccatacatga acgtggtcgg gcattgggag caataggtgc gtttgtttca ttaggctcga
     3481 ttgctggacc aggtcttggc ggcttgattt tgtcccaatt ttcttgggcg tacatttttt
     3541 ggatcaatgt accggtggga atcgtaacga tgttgattgc tgaaaaattc ttaccaaaag
     3601 atatcaccat gagcggccat acaatcgatg gagggggctt tgctgccatt gcggctacga
     3661 ttatcagctt ttttggagca atctttttgg ggcaagaaag tggctttttc catccagtcc
     3721 cccttgtact gttcgtaatt tcagtgattg cctttgtcag ctttttaaaa gttgaaaaga
     3781 aagcagcctc tccattgatt tctttcggta tttttaagaa caagattttt accatgagct
     3841 tgattacagc cttgttgatt ttttcttcca acttctttgt caatgtagtg attccttttt
     3901 atctgcaaaa tgctcgcggg ttgagtccta gctatgctgg gctgttgatg atggtctttc
     3961 ctttgatgat ggtcgtcggg gcgcctttaa gcggctacct gaccgaccgg gttggcccgg
     4021 agattttggt attagtaggc ttgggcttat tgtcggtaac ccaggtcatg tatatgtttt
     4081 tgtcagtgga ctctcctttg tggctataca ttgtcgctac tgccatcatg ggtgccggca
     4141 atgccttgtt ccagtccccc aataatacga tggttatgag ctcggtcact atcgaaaact
     4201 tgggactggc aggctcgatg aattcctttg cccgaaattt ggggatggtc agcgggattt
     4261 cgctttctac gacggtctta tatcaagcga tgtctgctaa gatgggccag caagtaacta
     4321 actatatcgc aaatcggccg gatgtctttt tatatggaat gaagattacc tttttaggca
     4381 gctttgtttt gtgttttgtt gcctttttcc tgactatctg gcgtatcaaa aaacaaggag
     4441 gtattcagaa aaatgtctga aaaaaccact gttgaagaat ttttggaggc ctgcgtatgg
     4501 gtgagtggga tttccatgga tgatggaaga tgaagtgaac ttctaaaagc cgaaaaactc
     4561 aagtattatc ttcaaaatac tagtgctcaa tgagatgctt gatttgatca caagaagact
     4621 gcgaaagccg ctggcaaccg caagctaggt atcccaccga tggactacgt tttagaggct
     4681 actggttatg aatacggagc caacgctctc atctgcataa gaatacatca cccagattat
     4741 gtaaagattt tcgatacttc tgtcaaaaaa tcattaagat agataaatga tttccgtcta
     4801 gtcaaattgg ttcagaatgt agacacacag atggtaagaa gtcactcaca attcgagaat
     4861 ggacttgtcc tatttgccgt atacatcata atcgaaacgt caacgatagt atcaatattt
     4921 tgaccaaggg ctatgaatac aagcattgcc ttagaaaaca tcaagaacct taggaacaac
     4981 ggggatagct tggttaataa gagataccgc tgtttaggct tgcaagtata ctcttttatc
     5041 aagaattccg tggctttagt catgagaggc ttaatactat gaaaattttc tttagcattt
     5101 ttgatttagg ttccttttct aattataata agatgaccac tacagtaacc tactgaaata
     5161 tataaacagc cactgtagta ccattttcaa agtagtctat gagaggagaa tcttcattct
     5221 tatgaacgct ggaattgcgg atatgaattt aataaaaaaa acccttaatg attttacttc
     5281 caattcaata tcaaaaggta ctggtatcaa tttaagcaca ataaaaaagc tgaaaagtgg
     5341 tgaaagatca gtggaaaagc tgaatctttt agatgctata aaaataacgg aatttgctat
     5401 gaaaaatgga aaagctgaga ttgaaatatg gagataatta tcgtctagtg aattacttgc
     5461 cacttagctg aatttaacga tccttggtgc ttgaaggggg agattcttgg gaatagagca
     5521 tacttgcaag cctatgtctt tactaacaac gatatctctt attaaccaag ctatccccgt
     5581 agttctaagg cttttacttt tatctaagca ataatcgtat tcaaaggaaa tttttgaccc
     5641 ttagaaagtc tcattttatc agacgtgagc ttaggcattg ctgaattttg ttaattcggt
     5701 tttaggttgt gctttgagca taatatgaaa gtattcttta tcacgatttc attcatctaa
     5761 ggtaatgtga tgtgattctg aaattctttc aaaggcagtt tttgcataag ctgacattac
     5821 atcatcaatg acatgtttac aatgtttcac tactaaaata agggggtaac caagaagaaa
     5881 aactgaatga ttatttgtat ctaattccat ggtatatcaa ccatttccta tatagactga
     5941 ttatgacatg gattaaaata aaaaggcact atgtcttaat tgtcggtttt cgagttaccg
     6001 aatggcattc atcacccacc tatagaggat gggcgacttc tgcctgaaat aagttacact
     6061 acttataact tctaataaaa ttagattgag tcattattat ttaacatcaa cgattttcca
     6121 gtttccattt ttttgtaata ctaatttgta ctgaaagtag ctagtggttt ttgatacatt
     6181 atcaaggtat tttatctttg tactcactag aaaatttttt ccgttttgta gataaactgg
     6241 attctctaac tccgaaaact ttaagtcaat attgattggt cgcatcgcac cattttcaac
     6301 ataatactcc aattcttttt cagaagctga tggataaagt ttaaaaaatg tttctaaaaa
     6361 atcggtgatt tcttccgttg ttttggcatc aatttctgaa tcgttttcta cttgttttgc
     6421 ttcatatttt gccttgtctg gctttccaat cattgttggc aaactagtta ctaaatgatt
     6481 cctgttttca tcttcaaaga ttgtcactcg atatgcagaa gaaattgatt gcgtcttttt
     6541 tccctctgta atatgttgct ccactaaaaa tgaaacgaca aattcttccg agtgtttttc
     6601 aacatctaga atttttacag attgaacttc ggaactcgtg ggaatatctg ctcgaaccat
     6661 atcttggatt aaagcgagac cgttttctgt aaggtactgt tcgagattac tcattctctt
     6721 ttcaattgct tctttgttat ttttccatga aaaatattct ttcgcaaaat cctctgtaaa
     6781 actttcaacg ccagaagtat tcactacctt ctcttttata accacttttt catgagttgt
     6841 atgttgatca atggcggtga aatttttata aacgccaaaa gcaagactac aagacaataa
     6901 aagccaaagg gcaagcacca tttttcgatg cttacccacg gatacaactc gttctttttt
     6961 cttttttggg ctcttttcct tttgattccg ttcaattttt atcttcattc tttttcctct
     7021 tttctcttat ttgtatcggc cagctccgat aagatgctgc cgccaatagt tttccgttag
     7081 attcgtccac cccactggat ctcctgcatg aaacatgcga ttctcccctg cgtaaatgcc
     7141 cacatgggtc acataagtac cggcatcata ggttgaatga aagaaaacta aatcacctgg
     7201 tttggcatct tttagatcaa tatgagtcat ggcatcatat tgatcctgag ccacccgagg
     7261 aagtttcagg tcaactttgg cataacacca ttgggtcaag ccactacaat caaaccccgt
     7321 ttctggactt gagccaccaa acacataggc cgttccttca tacttaaatg cctcatcaaa
     7381 aatttcttgg actattttgt catcaaactg agttgtagtt aggtattgct tcactaataa
     7441 acagtaaaac atattgccat aattataccg ccacccacca ttttccttaa tcgcgattgg
     7501 gttggggtaa tcgacttttt gaccacctga tttttccttt gaaaaagatt cagctaaagc
     7561 aaaactatgc ttcttcccgt ttttcccgac atgatctaga tacccgccac catagttata
     7621 ggcctgaata atactatctt gatcacaccc tagttctttg gaacgcttaa ccaaagacgc
     7681 aaaatagaga cagccttgtt tgatggatgc ctcggtactt aatgaattag gtggtaatcc
     7741 ggctgactca gaactttgca taacgtcttc cattgtgcca ccagattcca cttgaataat
     7801 cgccaaaaga attggaatat actctgttat atccgattct ttggcatact tttccaccat
     7861 tgcacggtgt ttcaatactt cttctgagac actagttccg ccaggagaag tgccagaact
     7921 accatttccc tcactgtctt caccaaacaa caaacccaca cacatcaaaa tacccataaa
     7981 agagatggtc agaaaagtta atgaaataac acccactttc ttcaagttca ttttttctca
     8041 cctgaccttg attttggacg atggttcatt cttttttgta ctttttgatt ggtctgcttt
     8101 ggcagagtgg acctctgttt cacttgttgg aaagaatttt tcttacttgc tgattggctt
     8161 ttcccttttg aaatgatttc ttgactagtt agttttcgtt tgatttcggg attcagattt
     8221 gctctcacaa tccgctttgg tgcttctttt ttcagcatca tatctttctg aggttgagct
     8281 gctttatcta ttggaatatt cgcccttctt tttccctgac gatttgttgc ttcactcagt
     8341 atcttgcgtt tttcgtccat tttcgcattt cttcgttgtc gttgtttttc tcgcaaatcc
     8401 actcgatccg ctttttcttg gaacagtccg cgcttgaatt cttctggtgc ttttttcgac
     8461 ttttcgattc ctttatggag attgtatttc aaattcgttg gcgtattcct tacctgatcc
     8521 tttacttgtt tagtcttatc tttcaccaaa gcttgggtat ccaaaacttt gccgattttt
     8581 ctcccagcca aattcattcg ggattgtttc ttattatttg aaactggatt ctccttagtt
     8641 aagtcagata gtatttcgtg atcgttcggt aatcgtgaat tctttcgtag agaagaaccg
     8701 gatggcttaa atttggattt tgattgttcc tttgccaact tatttccgac tgctgcacca
     8761 ccaccaaaag ccaaagctcg tcccacattt cgattgagtt gccgcaattt tcgattcatt
     8821 aacatttgcg gttttcgcat gacgcgtctt cccattgact ggctatcatt actctggagg
     8881 ttgaacatac tcataatgtc gcccaatttg aaatagatac cggcaaaggt cacaatttgc
     8941 agaaaagcaa ccatgaaaaa tggataggtt gccgaaatat tgaaaaacat cgtggagatg
     9001 ctaaatgcag tcgtaatcac caaagtaaca cccgctcgca tcataatcgt attgaataaa
     9061 cgaataatcg ccttctttcc taaactttca taagtcggca acatggacaa taagaaacta
     9121 attggcagaa acatcgcaaa gataatgaag agaatttgcg agaacaacat aatccctgtt
     9181 agaagaaaaa caaagaagga aatgccaata ttgaacaata ccaaaaagac cacggtccct
     9241 aaacggttca tggtcttggt aatactgaga ttcgcattgt cattgtcttc aatctctgcc
     9301 ttgaccgctt cttcccgatc tttgcctttg ttttcatctg gactcacaga tagaatcttg
     9361 ttgacccggt cttcaccaat ttcttctatg ttggaatcat caaattgcag tagtaaccaa
     9421 ggttgttgga cttgaatcga aaacaaacta tcccgaatca aatccacact atcttttcct
     9481 tggctttctg aattgggtac aacgatctcg gttccaaggg taagggccgc ttcactaaca
     9541 tctgagctaa agtcattgat ttttgtgata taggtaggcg cataagcaat gaacgaaccc
     9601 gatagtagaa aaatcatcac aaaattaatc acggctcgaa ctgctttcgt ggtttctcgt
     9661 tttaataatc cggtatacgc cacatacacc cccagagcta aaatcatcaa taatagaaac
     9721 ccaaaataaa atccggaggt ctggagtcca ttttcagtaa taccagccaa tgtttggata
     9781 ttctttccaa tactttcagc cgtatccgaa atgaaatcta atttgtaagc ctcttgaacc
     9841 acataacccg tggcgttcga caaatacaaa ctcaccgtcc aaatgaaatt agtaatggca
     9901 taaagaccat acatcacgct tttccctaag ccatcacccc aattccaagg caaccaatcc
     9961 caagagctat ccacaaagaa atctagttga tagttgttca gtgaatattt ggaatataga
    10021 tttcctgctt caactgtatc atcaactagc cctgtcgcct cggctaccgt accaactaaa
    10081 ctcaatagaa ctatcagagt gagtatgcta ataaaaacga ttcccacaat ccgaaaaagt
    10141 ttctttttca tcccctcact ccattctctc ttgaacaggt ggacgggtat caaaggcatg
    10201 gaagagttct tcaaagactg gatgtacctg aaccacgcct acccgaccat acaaatcctg
    10261 catcaagcat tggccatttt ccaaatctcg taggcgtttt tgattgcttt catcttctcg
    10321 atccaagcca aagaattcca aggttttctt gatttcatga atatcggtac tgcgaaacgc
    10381 aaacttcaac ccgatattat tcttcaagcg ttcatccagt aaatcatcac tattttgcgt
    10441 gacaaagtaa accccggcat tcatagcccg cccagcacga acgagcttat tcgataatgt
    10501 tttcccttgc gctacttgaa gaaaactcca cgcctcatct aagtctacaa ttttgaagat
    10561 ttctcgatta ctatggataa agtccaaggc aaaagtggaa atgacgatta gcatggaaac
    10621 actaagcaat tccatggtgg tatattcttc aaaagtggtt tctgcatctg gtaaaaccaa
    10681 atccgctact tgaatgatat ttaactgcct atctaaacta atcgactggg taatcgaacc
    10741 atctgagaaa agcaaatggg caaaatcata atcggtaaag gactcgatgt gatccgcgat
    10801 attttccgca actggtgagc cttcttttcg aagctcatta attacttgta ataatccccg
    10861 ttgattgctt tgggtaaccg tccgaatggc cttacgcaac actggaaact tctccccatc
    10921 tcgactagaa attcctgtta aaaaggtcag aatatcaatt gctagacttt cagaatcctt
    10981 ctttttctcc ataatcacat aaggatcaag taatccttga ttttttggct tactagttaa
    11041 gttgagaata ttgatttcat ccgcaatttc tggcaaggtt tctttccagc caccccgttc
    11101 cccttttgga tcgacaatca cagcttgtcc accaaaaagg acggcgtaat aaactaagag
    11161 attgttacta aaggactttc cgccaccgag tgaaccgagg aatgccgctg ctagcgcatt
    11221 ggtaactgaa tccttgaccc cttgtgaggc caatgccggt ttcaggtaaa cattgcgccc
    11281 cgtatctacg ttataaccaa agtaaatgcc ttccaactcc cctaacatct gagtggcgcc
    11341 aaaaccaagt ccggcgagaa aatcagacgt aacatactgg atataatcat tcatgtatcg
    11401 cttactagaa ggtaagaatt cttcatgtag ccccatcata tcgccaaaag gccggactag
    11461 ttttacattg gtatcatcat aaaaatctag cacttcatca caccggcgct ttagttcatc
    11521 tactgattcg gcacttactc gaaccacata gctcagtttg tacatggatt ctttggactg
    11581 atccaaggta gtttccaatt catctacgga atccaatgca tctaagacat tggaatttgt
    11641 ttcattgtca gattgatagg catggttgtc caagtctttt aattcctttt tcttattgcg
    11701 aacggtagca agtgcctttt tgttcgtcac aatctccaca ttcatactag tgtcaattgg
    11761 gaatgtaaat tgttgttgct ggtaatagaa aagttctgag gagggaaact ccatttcccc
    11821 cacaattgta ttaattgtta agtacgctac atatgattca tgattctcat gttccatgca
    11881 taagtaccgg ggcttttctt caatcaaaca acgactggga cgtaataagt cgtaacgttt
    11941 caccaaagtt tccgacttca gctttttctt tggtagctga aattcatatt cttcaaatgg
    12001 tgtacccttt tcgccataga tatgttcaat caaatacatc agatcactcg gtgtgactcg
    12061 tctgacttta aatcttcgag ccaatttact ttccattagt ttttcaagct tggtatatcg
    12121 gcgaatttct tcattggata aggaaacaaa atcgcccatc aaatgatgat tcactccata
    12181 gacaaattct tgaaagccgg agaagaaaga tttcttgaga ttctttagat tgacctcttc
    12241 atctgtcgca atcagtttga agccaataaa aaagcgataa tcaatctgac tatcgccaat
    12301 catggatact aatgcctctg tctgtaaatc aattcgttgt ttcgctactt ctgttaatcg
    12361 cccggtaatt tccttttttg atcgttcttg cgtggcacga atactacttt ctgtggcaat
    12421 ttgtaaggca tgaatcttgc cttcacgatt ttgcgcaatc aattgacgaa agttatcgtg
    12481 gacttgatat ttctgttcag gtgacaaaaa ggagtaattg taaggtatta actcatagta
    12541 ggcaaaacat tctccctctt gattaaagac aagattattt tcaatatact taatcgggta
    12601 catagttcac actccttacc agagtaatgg gttcgtccca tcgtttcttc tgttctttga
    12661 ctttctttcc tgcgaaagtg accttaaatc gtaagtgata gctaataaaa gatcgtaaaa
    12721 atcccaatgg tttcttaccg tcaaacgttt tttgcgacat gaaccaagta actgccaccg
    12781 gtatacctaa gtattttaaa aaggccccct ctatcataga tagtggaggc acatgggcaa
    12841 atacgatgac aaacagcaag gataaaacaa accaagccat ctggttaaaa gtcacaggaa
    12901 aaggcagttg aaagtcatta attgcataaa tgaccttttc aactgcccaa atactggtgt
    12961 agctttttat tttcttcatc aaatcattcc tttaaaaaga aggatagttt agaaatcaac
    13021 tacccttcaa taattttgtt aatatggata ctcaaaaaca cctcgactcg ttaccaaata
    13081 attgcctcca atctccaagt ctctaccaca cgcatcaaaa tcaatatacg tcttcaagtg
    13141 ttctggtact tctccaaaat caccagcttc tacaatcaag aaatatgcta catcactcat
    13201 atcatcgcaa tctggataat agataatatc atccacatgc tctaccatct cctcaaaaga
    13261 attgaagaaa gcatgttgaa tctcagaagc aacttcacca actggcgttc cttcaagttc
    13321 ctccgctaag ccacacaacc gattaatttc ctcaattggc gtatactcat caatctcaaa
    13381 gggaagttca taatcatgaa ttgcatattc ctcgtattcg tcatttaacc caattcgttc
    13441 tttcacttca tcaaaatcga caggtggggt aaaccacgcc cccaccaatt caccttcgtt
    13501 atatttcccg aggttcgcaa tataaacacg catctgttcc atcgtgcttc ccccttacgc
    13561 accgacaatt cggttaaaca aactgagtaa gacatccttc accccagaag cattaaagac
    13621 caatccgacc gcaatcaaag caatcaccaa aaagccaatc agtttggaaa actcccgctt
    13681 aaatcctaaa aacaaaccaa tgaccacaat tgccagtaag actaaggatt g
//
LOCUS       DAJSBL010000069        13398 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.071, whole genome shotgun sequence.
ACCESSION   DAJSBL010000069 DAJSBL010000000
VERSION     DAJSBL010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13398
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.071"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(14..706)
                     /locus_tag="ONT85_002411"
     CDS             complement(14..706)
                     /locus_tag="ONT85_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HCR2379361.1"
                     /translation="MQIELQHTHDKETFEKNYKVYFELARDSAMKYGIPLTLDTPYNQ
                     PGIKSHLWVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDDNPTTSEDKPVIDP
                     TRAGAANSTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELAR
                     VRADGIYRPDVNQAYNTLGNVGYHVSFNMRDFPNKKVYVMMRATNDPEGNTKGGAQDF
                     HDKRWYLNIPQR"
     gene            complement(1143..1712)
                     /locus_tag="ONT85_002412"
     CDS             complement(1143..1712)
                     /locus_tag="ONT85_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HCR2379362.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(1786..2109)
                     /locus_tag="ONT85_002413"
     CDS             complement(1786..2109)
                     /locus_tag="ONT85_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2379363.1"
                     /translation="MLLLKTLQERGYLDVHPPEVKIVFFFRYEHQEARRLAGVLNKTL
                     RHRKRISIHVCTRNKPPRYADLVIIDHFFPLDHNEDQKTYLYHPSFGIERLLTDINYW
                     LGKNR"
     gene            complement(2263..3075)
                     /locus_tag="ONT85_002414"
     CDS             complement(2263..3075)
                     /locus_tag="ONT85_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2379364.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLTKVLYQE"
     gene            3429..4277
                     /locus_tag="ONT85_002415"
     CDS             3429..4277
                     /locus_tag="ONT85_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HCR2379365.1"
                     /translation="MIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGTFSRVQCTPD
                     LLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLEAMAENHATI
                     DNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDDELLLLLDKF
                     EKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAIQLGISPRGS
                     LSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQ
                     IPIPVR"
     gene            4422..5363
                     /locus_tag="ONT85_002416"
     CDS             4422..5363
                     /locus_tag="ONT85_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HCR2379366.1"
                     /translation="MELDFIKQLSKETFGYLYIAATRLSQKKYAGYFFHSKKENFFFT
                     SPSFTNRLPSNLCRKKQEILILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFR
                     KSAKREISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNR
                     IDWKLSAHTQEWLYREYDYQEEECSPLFCFWGLPSPKFEPLLDLYFSLWHLRKEKQPE
                     LLILGNRAFHGKDPGHTYFASLEAGDEKAFFAHLKKYAKKQIVLFVPENSPEVSEAIE
                     TLSKDRTVYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            5353..7524
                     /locus_tag="ONT85_002417"
                     /pseudo
     CDS             5353..7524
                     /locus_tag="ONT85_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
     gene            complement(7636..9807)
                     /gene="copB"
                     /locus_tag="ONT85_002418"
     CDS             complement(7636..9807)
                     /gene="copB"
                     /locus_tag="ONT85_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HCR2379367.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIR"
     gene            complement(9827..12013)
                     /locus_tag="ONT85_002419"
     CDS             complement(9827..12013)
                     /locus_tag="ONT85_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HCR2379368.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGLIAVADQIKLEVKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLCLNRHMKK"
     gene            complement(12013..12222)
                     /gene="copZ"
                     /locus_tag="ONT85_002420"
     CDS             complement(12013..12222)
                     /gene="copZ"
                     /locus_tag="ONT85_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HCR2379369.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(12235..12675)
                     /locus_tag="ONT85_002421"
     CDS             complement(12235..12675)
                     /locus_tag="ONT85_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HCR2379370.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYYPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(12749..13288)
                     /locus_tag="ONT85_002422"
     CDS             complement(12749..13288)
                     /locus_tag="ONT85_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR2379371.1"
                     /translation="MKIIVIGQSGSGKSTLARKIKEITDFPLLPLDLLWHTTDYSIQA
                     KKWFLQEQQLFMVSNPSWIVEGNYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLK
                     RKINKKSRPDMPESFSEKLNREYLEFLSFIWHFEENNEPKIQQLIQVANARNKLIILK
                     TRREKQEFLTKLKIESKNN"
BASE COUNT         3765 a         2807 c         2380 g         4446 t
ORIGIN      
        1 agggggggat tttttatcgt tgtggaatat ttaaatacca acgcttgtca tgaaaatctt
       61 gtgctccacc cttagtattt ccttctggat cgtttgtcgc acgcatcatg acgtatactt
      121 tcttattagg gaaatcacgc atattgaaag atacatgata gccaacattt cctaaagtat
      181 tataagcttg atttacgtct ggtctataaa tcccatcagc tcttactcta gctagttctt
      241 ttccagtatt gtagtccata atgaaaatat actcgtattt ataattagca atgtgccatc
      301 cagctacatg caagtttgcg ttttcgattt ctccgaactg atcaatgtgg gcgtaatttg
      361 ttccatctgt cagcgtagaa tttgcagcac ctgctcgagt tggatcaatt actggtttat
      421 cctccgaagt agttggatta tcatccgtaa aaccatgagc caaatcatag gctagttttt
      481 ctttacttac acccatttca gaaagataac cgtaaggatc tgtatgatcg ccccaaatat
      541 tttgtgttac ccataaatgc gatttgattc ctggttggtt ataaggagtg tctaatgtta
      601 atggaatacc atatttcatt gctgaatctc ttgccaattc aaagtatact ttatagttct
      661 tttcaaaagt ttctttatca tgtgtatgtt gtaactcgat ttgcacagga cttttggctc
      721 acgaggttgt cctactctac atcttgctga tagatatgct gaaaggaaaa caccaacatt
      781 atgttttgct ttttcagcta gaacgagaca agcaggaata cctttagaag atatcaaagg
      841 ttttttaggt cataaagatg tttccactac acaagtctat gcccatattt ctcccgaagt
      901 gaaaaaaaga tcaatgaatc aactagaaaa ctatatagaa gagcaaataa aaaagcactc
      961 aaattgagtg cttttttaag ctcactatca ctaaaactat cactaatttt atttataagc
     1021 gaacatgttt ctatcactga ttttttaaat ctagcgaaaa acccttatct taaaggcgtt
     1081 cgttcaattc tttggcaagg tcttcaaagc ctggcttgcc tagaagtgcg aacatggttt
     1141 tcttatagct catgtagaaa aattttcttg atgatgattt tatccatcaa ttgagattga
     1201 ctgttttgct ctcctatgat ttcagctatg attgtccgat tttgcggttt tcttcttaac
     1261 tggtagttga ttttcccagt ttccatcaac tcgctaagca tatccacgac tccgccaaat
     1321 ccggtctttt tcgtaataaa acctagtgct accattcgtt catctagatt ttttgctgta
     1381 atcgtcacac cagtaatgag cgagtgctgt aatgtttggg ccagctcttc catcccttca
     1441 gcattggata cgacggaagc tgttttgtta ataggttctt cgtttatttc ttttggttct
     1501 ttcctctcca ctttcactgt ttgacgaagg acatctagtt cttgcaagag cgtttcgtat
     1561 tgttcttggg tcagcatcac gccagctact ttttcacgat taaatatata tacgccagtt
     1621 cccgcgtctc gcgcttgagc aaatacatcc atcggcgatc gtttcacttc tgtgatcgaa
     1681 gatgtgggaa cttctaattt ctttaattcc aatggatttc ctcctttact aatcgctttt
     1741 tattatagca cagactgact tgttttgatc ttttttccaa aggttttatc ggtttttgcc
     1801 taaccagtaa ttgatatcgg taagaagtct ttcgatacca aatgaaggat ggtacagata
     1861 cgttttttga tcttcgttat ggtctagtgg aaaaaaatga tcgatgatca ctagatctgc
     1921 ataccgaggc ggcttatttc gagtacatac atgtatagag atccgttttc gatgtcttag
     1981 cgttttgttc agtaccccag caagtcttct tgcttcttgg tgttcatagc gaaaaaaaaa
     2041 gacgattttc acttcaggag ggtggacatc taaataccct cgttcctgga gtgttttcag
     2101 taaaagcatc ccccactcta ctgcggggtg tgcaagtgct tgttggatcg ctagccgaaa
     2161 ttgatatagt ttatctttgg aattgggaaa tttttctaat gtttcctgat aataacgaac
     2221 ttcttctatc agccggtcat ggataccaat tacgtaacga agtcattctt ggtaaagtac
     2281 ttttgtcagc tttgatttgc tttcgctctc aaattcgcta ttcagccaaa acagcgaatc
     2341 aagcttttca gtcacaatcg tcaatttccg atagtcttgt gtctcttcta tccgtttcca
     2401 ccgcttttca gtaatctggt tacagctcaa gcgcaaaatt gctagataat ttttttcttt
     2461 tattggcaaa atctgctcct ctctaaatag caattcagga aacaattcaa agaatagtgt
     2521 tcgactgtct cctaaaatct cgatttctgc cggatccttt ctttgcttca ataattggat
     2581 gaacatctca gaaaaggact gctctccatc atctgcaaaa aaatgcggaa aatctttcaa
     2641 aaagcaaaac agcagatatc ttacttttct ttctgaacca aagatctgtg cttttttata
     2701 agaaaaagaa aggtggtact cgccaaacca ctttctcaat cgctcttgcg tagaacgatc
     2761 gtacttctgc tcgtaaggtt cattcaatag cagatttttc agtaaacaaa acgggggtga
     2821 ttggatcaga aaaacaaaca agagataagg ttcgctatat ccttgatcat aaactaactg
     2881 aacggttttc ttatgaagaa tcagctgaat gggacagtcg ctttccgcca aacgtttcag
     2941 atgccgatcc actgttttaa catcgatttt cagttcttca gctaaatcag cttttcgcca
     3001 gatcaaatct ttcattagat tgattagtga aaataaattc tgtgcatttt ttgataatag
     3061 tttttgccat ctcatcgatc atttctcacc tgatttctaa acatattgtt tgatcaaaat
     3121 gggttcactc ctaaaaagat aaaaaactcc ctttctattt atttaaatac gcaaatcttc
     3181 ctcattattt tttattttac tcaatttaat cgtattctca tttcaaaacg aatgtacttt
     3241 aaaaaaacat aataagatca ctttgcttgt atgatgtatt cccttccatt acaatgaaat
     3301 aaatagcgat tttatgagaa ggtgatgtga aaatggaaga aaaaatagaa gaatccaaac
     3361 aaaaaatcga tcagttgata taagaattag aaaaagcggt catcagcaaa cgttcaacta
     3421 tccaattgat gatcgtggcc ctattagcag gtggtcatgt cttattcgaa gatattcccg
     3481 gtgtaggtaa aacgttactg gtcaaagcac tagcaaaagc tgtacaggga accttcagca
     3541 gagtccaatg tactcctgat ctgctgccta gtgatattct tggtttttcc gtatacaatt
     3601 cacaatcaaa agagtttgaa tttcgtccgg gaccgatctt taccacgatc ttgttagcag
     3661 atgagatcaa tcgtacgact cctcgtactc agtcagcact tttagaagca atggccgaga
     3721 atcatgcaac gatcgacaac aatacatacc ctctagacaa tcattttttt gtcttagcta
     3781 cgcaaaatcc aatcgagtac gaaggaacat atcctttgcc ggaagcacag ctcgatcgtt
     3841 ttttattccg actacaaatc ggttatcctt cttttgatga cgaacttttg ctacttttgg
     3901 ataaattcga aaaagtgttg gataacttaa atgaagtatt gaacagttat gaaatagaag
     3961 aaataaaaac aaatgtggcg aatgtctttg tcagtcccga agtggcatct tatgccttgc
     4021 agttagtgtc agcaacaaga agtcatgctg ccatccagct tggcatcagt ccacgaggaa
     4081 gcctttcctt tattcaggca gcaaaagctt atgctttaat ccatgggaga aattacgtca
     4141 cgcccaaaga tttgcaggat ttagttcctt acgtgttttc ccatcgcctg attttttacg
     4201 atcgttcatt gaaggaagac gaaaagaaac aaatcattca tactcttgtt gcacaaattc
     4261 ctattcctgt gaggtagatt gaggtagaca atggaaaaaa tcaaagatta tttcattaat
     4321 tttgtgttat ttctggtata tttcatcatt gtgctttttg ctcttgtatt caacaattct
     4381 ttaggctggt tcttgttggc cttttttact ctttatgtat catggagctt gatttcatta
     4441 agcagctttc taaagaaact ttcggttact tgtacattgc agccactcgt ttatcgcaaa
     4501 aaaagtatgc aggttatttt ttccatagca aaaaagagaa ctttttcttt acctctccct
     4561 cgtttacaaa tcggcttccc agcaatcttt gcagaaaaaa acaagagatt ttgattctta
     4621 caaataaacc gcaagaagtc caattgcttt ggcagccaaa agaaagagga tgccatgaaa
     4681 cattaactgt catttttaca gcctatgata gtctaggact tttcagaaaa agtgcaaaaa
     4741 gagagatctc ttttcctgtg actgttctgc ctgctttttg taagattcaa gcagaaaaat
     4801 tacagagagt tctggaaaaa aaacagcagc tgaacgcgta ccaaagaggt cttgattttc
     4861 gggaacatcg cgcttatcgt ccaggtgatt ctttcaaccg aatcgattgg aaattgtctg
     4921 ctcatactca agagtggtta taccgtgaat acgattatca agaagaagaa tgttctcctc
     4981 ttttttgctt ttggggtttg cccagtccaa aatttgaacc tctgctagac ttgtattttt
     5041 ctttatggca tttgagaaaa gaaaagcagc cggaacttct gatattaggc aaccgcgcct
     5101 ttcatggcaa agaccccggt catacctatt ttgcttcttt ggaagctggc gatgagaaag
     5161 cttttttcgc acacttgaaa aaatatgcaa aaaaacaaat cgtcctcttt gttcctgaga
     5221 attctcctga agtctcagaa gctattgaaa cattgtcaaa agatcgaacg gtttatcttg
     5281 tttatttcgt agaaaaacaa ctcatggtcc aagaaaaaga caagatatgt tcattgccag
     5341 gaggtgaatg gattgacgat taaacgggaa aaatggctgc gtggcgttta ctcctttgtg
     5401 atactaagtg taggtgctcc gatatttgta gtagctaatc aattgcaaaa cagctacctt
     5461 cttatcctaa ctattggtgt cctatgcctt attgtctgct gtattcctaa aaaatgggta
     5521 cgtttgctgc tttctttgcc agtaatgata ggttgtttgt ttttatattt tccttcgaag
     5581 acattttcca tattatggat cttacagttt ttttcgtcag tcacagagga attcttccat
     5641 ctatcccaag gtaaacttta ttatttgcca gaaaagacag ctttttttct cataatgttg
     5701 ctgatctatt tatttattac attgaccgtg gattacgact attggtacgc accctttctt
     5761 tctttgatgg cttacttcat gatgcttacc gtttttcgga aaaaagattt actgtacgaa
     5821 atgattgcgg tagtaactgt tttgttcgtg tatctggcag caaggcaatt tttctccatc
     5881 cggttgttgt cgggaaacac tcgattccag tcattgacta cagttctgct tatttgtttc
     5941 ttaactttta gcgctatctt gcctttatat cttccgaaag tacaccaatc tctcgaggaa
     6001 acgagtgaac cgatccgaga atatcttaat gatgaagggc tttatgacac actccatgag
     6061 tatcaattaa ccggaagtgc aagaacaggc ttcagtgaga atgatgaaca gcttggtgga
     6121 cctttatatg ataatggcga agttgttttt acagcgaacc aaaaaggaaa tggctattgg
     6181 aagatcgaaa ataaaaatca ttacaccggt tctggatgga tagaagatca ggacgcaccc
     6241 atccaaccaa tccaagaatt ccctgttgac atcacgagct accaaaccgt tatagaagac
     6301 aaagaaaaca ttacattgac cctaaaaaaa caagttttct taccttatcc ttatggaaat
     6361 actaccttga ctgatttttc attagctagt ggacaaacgc tttactattt gcctgaaagc
     6421 cagcgattca ttcctgtccc gaatgaatcg acacgttctg caactttaca aacagaaagg
     6481 ttagttttta cgagagaaga attaaaaagc agtcagttaa aagctgatga tttagaaaat
     6541 cagcagacaa atcttcaatt gccaaaaagt ttgcctaaac gtattgagga cttggcggtc
     6601 gaattgacca aaaatgaagc aactttaatt cgtaaagtcg aagctgtcta acagtattta
     6661 aaaacaagcg gaaatttccg ctattccaaa acggataccg ggcatactcc aaaagaacac
     6721 gattatgtgg attatttcct ctttgattca aaagtcggct attgtgacaa cttttcaact
     6781 tctatggtcg tccttcttcg aacacttggt atcccagctc gctggacaaa aggttttaca
     6841 aatgggctca gaacaggcac agataatcaa gggaatgagg aatacagcat tctcaatagt
     6901 catgctcatt cttggccaga ggtatacttt cctggatttg gctggcttcc atttgaacca
     6961 accccttcat ttgccaatcc aatgggttca caaccagttg ctgaaacaac cgaaaccgtc
     7021 cagcctgcta tcgaacaacc aaatgaaagc gaaaatatgg cagaaactat ttccagtgag
     7081 agatctgagg tcattccatc agaagaaaca acactcacac cagcttataa aatcgtctta
     7141 ttcagccttg gagtgtgttt atccgtcttg ttgttctttc tattgttatt cagaaaatct
     7201 ctgcgctgga caagttatct tatctatctt catttttttg cttctactaa tacgattgca
     7261 tatcaaaaac ttcttaaccc tttggaaaca ttggtaccgc gcaaacccgc agaatctttg
     7321 caggaatata gcctgcgaat agaacaagaa ttcaagctag atctgcgttt cattcgatta
     7381 actactcaat atgaagcaga aatatatgga cgacaagcag atccggtaaa attcgataaa
     7441 aaaacctatt tagaaataac gcatataata agaaaaatgg tatacaaaaa aattctgaca
     7501 aaagggagcc gcttcaaaaa ataagataaa atttctggag aatctacttc tggaatgccg
     7561 agaaccagct gattgagtat cgattactcg aaaaaaaggt tgtgacacgt tttttgtcac
     7621 aacctttttt cattattatc tgattttcaa tgtcaaagca tttagagcta caactaccgt
     7681 acttaacgac atcaagacag caccaactgc tggacttaat atgataccaa ttggcgcaag
     7741 aattccggct gctagcggaa ttgcaagaat attgtaacct gccccccacc agaggttctg
     7801 gatcattttc tttcgcgttt gcttggctag atcaagaaaa tgcaaaatat cttttggatc
     7861 gctgtttgtc aatacgacat ccgcagaatc gattgcgata tctgtccctg caccgattgc
     7921 catcccgatt gaagcacggg ctaaacttgg cgcatcattg atcccatcac ccaccatgat
     7981 tacttttttc ccttggtcaa ggtatttctg gacaacagct tctttatcat ctggtaataa
     8041 accgccataa tattcttcga tccctaaata atctgctact gcttgggcag cgtttttatt
     8101 gtcaccagta agcatgacag gagtgatttt tctttctttc aatgttcgga taaattcttt
     8161 tgcttctgtt tttactacgt cacctaaagc aacgattgca gctagatgat tttctaaaat
     8221 caggaagctg atggtatttc cttgttcttg gtaaggtttc aataatgctt gttctacgtt
     8281 taatccgaga cgctcaactt ctttttcatt aactattttc acttgttgtt ttttgactgt
     8341 tgcctctagc ccgactcctg aaagattttt tagattttca gcttgataag gcttgatttc
     8401 atgttctttc aaataattca taatgccaac agccaatgga tgattggcat tcgcttccaa
     8461 ggctcctaga tattgaagga tttcattttt attgaacgca tcatcaagta cttcgacacc
     8521 cgttacagta aattttcctt ctgttagtgt tcctgttttg tccagcatga tcacatctag
     8581 atcattggct tgctccagtg cattgcggtt tttcaaaagt aatccgtttt tagctgctat
     8641 tgatgtggaa cgagcaacaa ctaatgggat agctaaacct agagcatgag gacaggcgat
     8701 gataaagact gttaccatac gttctaatgc attaggaaga tctgtcaaga acaaccaggc
     8761 gatgaatgcc aggataccca caattaatgc gacataaaac agccattttg ccactttgtc
     8821 agataatgac tctagcttgg atttttctcc ttgtgctttt cgcaccattt ccataacctt
     8881 cgctagataa ccattttcac cagttccagt tacttcgatt tcgatcgttc catccccatt
     8941 gatagaaccg ccaatcactt tgtcattggc ttgttttttg attcctcttg attcgccagt
     9001 cacagctgat tcgtcaacaa ttgtttcacc tttcaagatt acaccatctg ttggcatttt
     9061 atcaccagac cggacgatca aacgatcacc ttcgtttact tctttcaatg aaactgtctc
     9121 ttcttttcca tctttcgtca agcgtttcac tgattctggc agtaattctg ctagtttttg
     9181 taaagcattg ctggcattcg acacagcatt catttcaatc cagtgaccta gtagcataat
     9241 aacgatcaaa gtagccaatt cccagaagaa atccatgacg tgtgtatgtg gattgagcag
     9301 atttgcgata aacgaataga cactataaat atatgcaact gtaatcccca tcgcgatcag
     9361 tgtcatcata gcaggacttt tttgtttcaa ttccatctta gctccactta aaaatggctg
     9421 accgccatag ataaatagta tagttgctaa gatcaatacg agccaatcag aaccaggaaa
     9481 cgttacttga aatggaaatt ccattcccat cattggtgaa aaaagaatga ttgggatagc
     9541 taggataagc gatagccaaa atttttgttt gaaattcccc atgtgcatgg aatgatccat
     9601 gccgccgtgt tcgtgatcca tgtgcatcga atggtccatt tgtttgctgt ttgctatatc
     9661 tccatggtta tgtcccatgt gcatggaatg atccatctga ctatggtcat gtttcatctc
     9721 catctgtttt tcatgagatt ctgtactttc tttgtttact acttcttttt tatgctcatg
     9781 ttttttctcc atatcgttcc cattcatttt tactccatcc cttctcttac tttttcatgt
     9841 gtcgatttaa gcacagggag ttcaacaata cactgacgga actaaacgcc atcgctcctc
     9901 ctgcaatgat tggattcaaa aagcctagtg cggcaaaagg aataccaatc gtattataga
     9961 caaaagccca gaaaagattt tgtttgattt ttctcaatgt caacctagac aactcgattg
    10021 tttgtgcaat ggatgccaaa ctgctattca tcagcgtcac atcggctgtt tccatcgcaa
    10081 tgtccgttcc acttcccatt gccataccga catcagctaa tgctaaggcc ggtgcatcat
    10141 tgattccatc gcctgccata ccgacttttt tgccatcttt ctgaagtttt tctacgtagg
    10201 ctgctttatc ctctggaagt acttcagcaa agatatgctt tggatcaata ccaacttgtt
    10261 taccaatcgt ttcggctgcc agtttgttgt ctcctgtcag cataaaaaca tctaatcctt
    10321 tattctggag ctgcttgatt gcttgtttga cttctagttt gatttgatca gccacggcaa
    10381 tcaacccgat cacttgcttt tcatcagcta aaaacatgac tgtttttccc tgctgttcta
    10441 attctaacgc gtgttcttgg tattcatcaa atgataggtt caattcaatt aatcgtttac
    10501 gtgtacccgc aaaatagcgt actccgttaa tcgtaccgct gattccagca cctgggtggg
    10561 ctgtaaaatc aatcatatcg tatgttgctg cttcttgcag ttttccgtat tccacgattg
    10621 ctttacctag tggatgttca gaagcttgtt ctaaagtata aaaaatagat aggacttctt
    10681 tagaaccgac tagatctgta actttgggct ttccttctgt aatcgttcca gttttatcca
    10741 aaacaatgct gtctaagtga gcagctgctt ctaatgcttc tccgccttta attaaaattc
    10801 catttcgtgc accaacaccc gttcctacca taatagctgt tggagtcgct aaacctaatg
    10861 cacatggaca cgcaatgact aatacagaga cactatggat caatgcgagt tgccaatcac
    10921 cagtgatcaa acctgtagct atgagcgtga taaacgcgat gactaatacg atcggaacaa
    10981 aaatccctga gatccgatct gcgatttttt ggattggggc tttgctccct tgagcatctt
    11041 ccaccatttg aatgatttgc gctaatactg tctgcttgcc gacttgtgta acttccatgt
    11101 gaagtagacc attcgtattg attgtcccgc caaagagcgt gtcatccgga ttcttttcta
    11161 caggaagatt ttcaccagta agcatacttt catctatcgc actgcttcct gaaataattc
    11221 gaccatctgc tggaatctgc tcaccaggac ggatgactag ttgatcacca acgactactt
    11281 cttcaatcgc taacgtttct tctttcccgt ttcttatcac ttgggcggtt tttgtttgaa
    11341 gagacatcaa ttgtttgatt gcattacctg ttttcgtttt tgctgtatgt tccaaatatt
    11401 tgccgagcaa aatcagcgtg atgatcatac tgctgctttc aaaataaaga tcttgggggt
    11461 gtccacggaa aaatccgttg taaatgctca atgcaaaggc agccgatgtt ccaattgcta
    11521 ccaatacatc catatttggt gctttcgtct tgattgcatg gtacgcacct ttataaaaac
    11581 gagcaccgac ataaaattgg acaggagcag acagcactaa ctggacgatt ggaaaatgaa
    11641 aaaagtgaac aatcgccgca tggcttccta acatcatagc aatcattgac aacatcaacg
    11701 gcaaagtcaa aaccgtactc aatataagat cgaatagcat ttttttcaaa tacgcttgct
    11761 tttcttctgc aattttttgt ttgtgggctt catcgtacaa aattgcacca tagccgatgt
    11821 tttctacgct ttggattaat ttttcagtgg tcgtcccttc atatttgaca cttgcttttt
    11881 cagtggccaa gttgactgtg gcattgacca caccaggttg tccgttcagc tctttttcga
    11941 tcctagcaga acaatttgca caagtcatac ctgtaatgac gaacgtatcc attttggttt
    12001 ctgctgttgc catcaaatca cctctgcttt ataacccaat ccattgattg cttgacaaat
    12061 cttttcggat tgaacttttg tttcatcaaa cttgaccgta ccgtttgctt ttttcaaatt
    12121 tactttcact ttttgcacgc cgtcaagtgc gcttactgtt tcttctactc ttgctgcaca
    12181 atgattgcag ctcatacctt cgattgaaaa ttgttgtttc attttttctt cctcctattg
    12241 atgttgttta cattcacatt gacctgggat acagttacat tcgatcgtat ctactggttg
    12301 cttttgttcc agttgtttca aaatctcatt gatatcttct tgagtcaaag tggcttcttc
    12361 tactagatcc gcaatcgtct ctccaatcct ttttgcacag atatgggaaa aaagattttc
    12421 cgtcgcactg cgaactgttt ccatctccga gacagctgga taataaatga atttttttcc
    12481 ttcttgttct gtccagatca cttctttttt taccaaacga cccagcaaag ttttgatcgt
    12541 tgctggtttc caatccatcg aatcagctaa gatctgcgtg atttcttggg ctgtcgtctg
    12601 tccaagtgtc caaataacac gcatgatctc ccactctgaa tcactgatct ttactggcgt
    12661 tttgactttt tccatttatt tcacaccttt cgtttacaaa tgtaatcatt atcttatgaa
    12721 tacagattac acttgtaaac aaaaactgtc aattattttt gctctctatt ttcaatttag
    12781 ttaaaaattc ttgtttctct cgtcttgttt taaggatgat cagtttattt cttgcattag
    12841 ctacttggat cagctgctgt attttcggtt cattgttttc ttcaaaatgc cagataaaag
    12901 ataaaaattc taagtattcc ctgtttagtt tctccgaaaa tgattcaggc atatctggcc
    12961 gagatttttt gtttatcttt ctttttagag aacgccaaac tactcggtag atggcgagat
    13021 aacgcggcac tttaagccaa atgatcttat ctgcttcttt aatcctttcc tctaatgtag
    13081 aagtataatt tccttcaacg atccatgacg gattagatac cataaacaat tgctgttctt
    13141 gcaaaaacca cttttttgcc tgaatactgt agtctgttgt atgccataaa aggtctagcg
    13201 gcagtagcgg aaagtcggtg atttctttga tcttccttgc gagggtcgat tttcccgacc
    13261 cactttgtcc aatcacgatg atcttcattc ttctccctca cttctctttg gaatttggtt
    13321 cgatcatacc acttataacg ccgcaaaaaa acaagggact gtgacaaaag tcgtgtcaaa
    13381 gtcccttgtt tcgaataa
//
LOCUS       DAJSBL010000070        13328 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.072, whole genome shotgun sequence.
ACCESSION   DAJSBL010000070 DAJSBL010000000
VERSION     DAJSBL010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13328
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.072"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            217..1956
                     /gene="yfcC"
                     /locus_tag="ONT85_002423"
     CDS             217..1956
                     /gene="yfcC"
                     /locus_tag="ONT85_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HCR2379372.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            2113..3297
                     /gene="iadA"
                     /locus_tag="ONT85_002424"
     CDS             2113..3297
                     /gene="iadA"
                     /locus_tag="ONT85_002424"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HCR2379373.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            3408..3611
                     /locus_tag="ONT85_002425"
     CDS             3408..3611
                     /locus_tag="ONT85_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HCR2379374.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(3712..4374)
                     /locus_tag="ONT85_002426"
     CDS             complement(3712..4374)
                     /locus_tag="ONT85_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR2379375.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(4387..5889)
                     /locus_tag="ONT85_002427"
     CDS             complement(4387..5889)
                     /locus_tag="ONT85_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR2379376.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            6437..8116
                     /locus_tag="ONT85_002428"
     CDS             6437..8116
                     /locus_tag="ONT85_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HCR2379377.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            8243..8722
                     /locus_tag="ONT85_002429"
     CDS             8243..8722
                     /locus_tag="ONT85_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HCR2379378.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            8941..9621
                     /locus_tag="ONT85_002430"
     CDS             8941..9621
                     /locus_tag="ONT85_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCR2379379.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            9629..10960
                     /locus_tag="ONT85_002431"
     CDS             9629..10960
                     /locus_tag="ONT85_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HCR2379380.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            10975..11937
                     /locus_tag="ONT85_002432"
     CDS             10975..11937
                     /locus_tag="ONT85_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HCR2379381.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            12048..12386
                     /locus_tag="ONT85_002433"
     CDS             12048..12386
                     /locus_tag="ONT85_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HCR2379382.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            12465..13172
                     /locus_tag="ONT85_002434"
     CDS             12465..13172
                     /locus_tag="ONT85_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HCR2379383.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
BASE COUNT         4266 a         2340 c         2916 g         3806 t
ORIGIN      
        1 taaaaaaaga attttaagga aaaacagaga aataaaatac ttaatataaa tggaaagaga
       61 aaaaaataat catacatagg agaaaaaaaa caaaatagaa aaaagtgatt ttctaataaa
      121 ttattcatgt ggaaatgtca tttttactgt ataattcatt tgattatcca ttttttattc
      181 atcaaaatta ctgaaaaaag aaaggaagaa ataaatttga agaaaacaaa ttcttcagga
      241 aaaccgaaac gcgacttcac taaattatcg acaccccata cgtatgtcat tatttttggt
      301 gtagtcatct ttgcatggat actgactttt gtcgttccag caggaaagtt cagcacgcaa
      361 gatattgaat acaaagatgc aaatggcgaa acaagtactc gtactgtttt acgtcaagat
      421 tccttccgtt atgcttacga gctggataag tcgtatgtgt ttgatcaatt agaagaactg
      481 caagatcatc cagcagaacg tgaaaaactg gatgttccgg aaaaaggatt ggaaaaagtc
      541 attgctgacg gagaaaaaaa tcttacgcag gaaaaactag atgaaatctc cttgacagat
      601 gatgtgcttt acgatcagta cggcgaaaat atctatgata cttctaaaaa attgcataaa
      661 acagccaaaa tatggggaac agatgatttt ggcggctttg gtttcttaaa ttttgttttt
      721 gaaggactag tatctggaga taagtatgga tctgctgtag ggatcgctgc attgatcctt
      781 gttgttggag gtgcttttgg tattattatg cgtaccggag cgatcgacgc aggtatctat
      841 gcgtttatca gtaaaactaa gggactggag cgtttagctt taccgctatt attctttgct
      901 ttttcattcg gtggcgctac attcgggatg gcagaagagg taattccatt ctcaatggtg
      961 atggttccat ttgtcattgc gttaggatat gactcgatcg tagcagtaac cgtgacttat
     1021 gtcgcatctc aagtcggaaa tgcgacctct tggatgagcc cgtttagcgt agcggtggct
     1081 caagggattg ctggaatccc cgtgttgtct ggtgcgacat tccgattgat tatgtgggta
     1141 gttgttactg cattagccgc tggttatatg atgatatatg cagaaaaaat ccgtaaaaaa
     1201 ccagaaagtt cgttgactta caaatcagat gattatttcc gttctcatat caaaaagaca
     1261 tctgatgaaa ataaaaaatt catgcttggg cataaattga tcttattaga gatgttagtt
     1321 gtccttgtct ggatcgtttg gggcgtgact caaaaaggct attacattcc agaaatcgct
     1381 tctcaattct ttgtgatggg attagttgct ggaatcattg ctgttctttt caaattagat
     1441 ggaatgaaag tcaatgacat cgcttcttct ttccaatctg gtgcagctga tttagctggt
     1501 acagcaatcg tagtaggtat ggccaaaggg attcttcttg tcttgggagg atctgatgca
     1561 tctgttcctt cagctttgaa tacgattttg catgggatcg ggacggcgtt gaccggtgtt
     1621 ccagctgtca ttggtgcttg ggcgatgtac atcttccaaa gtttgttcaa cttggttgtt
     1681 acatcaaatt caggacaagc agctttgact atgccaatca tggctccact ggcagattta
     1741 gtaggtgtat ccagacaagt agcagttctt gcttatcagc ttggttcagg ttttatggac
     1801 gcttttacac ccgtatcagc cagcttgatt ggtgtgctag gtgttgcacg gatcgaatgg
     1861 gcaaaatggg caagattcca aatcaagatg caaggattcc tgtttgtttt aggaactatt
     1921 ttcatcatga ttgccattgc aataggtctg cagtagtact ggagacctgt aaataaaaaa
     1981 gtaatccaaa cagtagataa ggactccaag aaccgtcatt ttacggtaaa atcaaaggag
     2041 taaagatcct atcgtttgga ttatttttta agatcctatc atcagttata cttaaagaaa
     2101 gaggaaaaat gtatgaaaat cattcgacaa attgaagtgt ttgcaccaga ttacttagga
     2161 aaaatggatg tactgattgc cggtgggaag attttggctg tagaagaaca gctagaaggc
     2221 ggctacgaag gagtggaagt agaagaactt tcaggtgagg gaaaagtact aaccccgggt
     2281 ttcattgact gtcatttcca tattttaggt ggaggaggag aaggcggtta tcaaaatcga
     2341 acgccagaag tcacattgag ccaattgact acagctggtg taacgacagt tgtcggttgt
     2401 ttgggaacag acggagaagg tagagatatg actgcgttga tcagcaaagc gaaaggtctg
     2461 gaagcagaag gaatctccac ttatgtatac gaaggttcgt accgattgcc ggtaaaaaca
     2521 gtaacagatt ctatcatcaa agactttttg acaattgata aaattatcgg aatcggagaa
     2581 atcgcagtat ccgatcaccg ttcttctcag ccaagttttg aagagttcgc tcgtgcagta
     2641 gcagatgctc gtgtaggagg catgttatcc ggaaaagctg ggatcatcaa tgtccactta
     2701 ggcggaggaa aaagaaagct agagctattg acacgaatcg tagaagaaac agaaattccg
     2761 atcacacaat ttttgcctac tcatgcaaat cgcacaccag aattatttga agcttgcgtg
     2821 gcatttgcca aacgtggtgg cacgattgac ttcactgcta gcgaagatcc tgatttttgg
     2881 gagaagacag atggtgaagt tcgcttcagc aaagcactga aacgattgat cgaggaagat
     2941 gtttctttag ataatttcac gatgacctca gatggacaag gaagcttgcc ttattttgat
     3001 gaaaacaatc atttcctagg gttaggagta ggaagtgcca aggcattgct agtagggatc
     3061 aaagaagctg tgcaaaaaga aagtatccct ttggaaatcg ccttacgagc tataaccagt
     3121 aatccagcac gtatattgaa attggataaa aaaggaaaaa tcgaaattgg tgctgatgca
     3181 gatctgtgta tcttggataa agaaacgctt gatattgata cagttatcgc taaaggagaa
     3241 ataatggtcc aagaaaaaga agtcaaagta tgggggactt tcgaaaaaag tttttaatca
     3301 acagctctaa ctatcttttt tagaaaacga gcgttctgtt tgtctgtatc tgttgttgat
     3361 gttaccctga aggtagatca ataaacaaac taagaggagg aagcacaatg gcagatttaa
     3421 aaggacgttc taaagatatg aaagataaag tagtcggtga aggaaaagaa gcatacggca
     3481 aagcaacaga cgacaagagc aaagaaacag aagggaaagc acaatcacta ggtgctgaca
     3541 tcaaagaaaa agctcgaaat ttaggcgacg aagtcaaaga caagttttct cgaaaagacg
     3601 aaaaaaaata aaaaccactt attactaatt gaatcaagca aataaaaaat ccgaaccata
     3661 tctgcgagac agatatggtt cggatttttt atttgatcat tatcctgttt tttaaacttc
     3721 gattttttga aattttttat tttttagttc aaagacttca tcacagcttt ttgctacttc
     3781 aatctcatga gtcacgatga taatacagcg attttgttca tgggcgattt cttggaaaag
     3841 tttgacgata tcttgtgaag ttgtttcatc tagatttcca gtaggctcat ccgctacgat
     3901 tagttgatgt tcacagcaga tagcacgaac gatagcgact cgctgctgct gacctcccga
     3961 aagtttggtc acctgttttt ttgctaatat ttcatcaatt cctacacgag ccagtcgatc
     4021 taaagcaact tgtcgataat tctcttgctt ggtcttagtg atttccattg ctgctatgac
     4081 gttgtctaga gccgataaat aaggaagcaa gttgtaagct tggaaaataa tcgagacatc
     4141 atttttacga taacttctta acccgatttt gttcagtgaa cgatcttgat agtatacctt
     4201 tccttctttt ggtgcatcta gtccagaaat aagcgaaaga aaagtcgttt ttccagaacc
     4261 acttgctcct aaaatagcgt acatttttcc agcttcgaaa ttcagttcta catctttata
     4321 aagcgcatct tgcggatctt gataccaaaa tcccaattgt tctgtccgta acatcaaatt
     4381 cctcctttag tttaatagga tttttcttgg gtttaatctt aatatcccta tagaagctaa
     4441 aaagacagaa aacagactga ttccaaatgc tactccggct aatagtccca attgtgcagg
     4501 tttgacattg atttccaatt cgctgacttg atcagaaaca gcaaatggat tattcgtggt
     4561 tccttgttga ccatcgcgtt gaccgccttg ttcttgtctg ttacctggca tttgtccacc
     4621 gccaggagct tgctggtttt gtgtcgtggt ttctgtctgt tggtcaagta gctgctgtcc
     4681 cacggcattc gcgacgacgt ttccgctaag cgaagcaact cctaatgcaa gaatcataca
     4741 aatagccact tcggtaaaca attggagaat gattttactt ctggattctc ctaaagatag
     4801 caagacgcct aactcatgcc gacgctcccg aatggtaatc ataatgatca gagtaagtat
     4861 gataataccg gctacagcca ctagtaaaac aacattttta gaaaaacttg ccacgttgtt
     4921 taaaggttcc aacattgact gatacatgga atcatttgat tgtaagctaa atgtatctgt
     4981 gtcgatcagc ttctcagctt ctttgagaaa ctcatccatt ttatctggat cagatagagt
     5041 gtaaatagca gaatctatcg tgttatcgtc agatgtccca tttagttcat ttgcaaaagt
     5101 gtaagaagtg taaagtgtat tcgacggatt catgaagttg aatttcatac ccatactgcc
     5161 gcttgtttcg ctggattcat aaattccttt gatcgtcatt tcataagtgg tatcttcatc
     5221 ttcagtactc gtgattgtga aagtatctcc gactgccagg tcatttgttt ctgctagagt
     5281 agaatcaatg actacattgt ttgtgccttt gtcgctgtca tctattcctt caccatccgt
     5341 gatttttgct gttccatcag aaaaattact attttgtgca gttgctgaca caccactgat
     5401 ttgaaagtct gcttgagtca tttgcggatt cccgcccatt cctccagcag gttcaccgcc
     5461 tgcatcagta gtttctgaac tgtcttcact tgaatcagaa ctagtgatgg cagttattcc
     5521 atcttttgcc atagctgagg cggaactttc aaaggaataa ctctttacat tatctaattc
     5581 agctattttt tccgcgtccg ttaaggaaac tggtgtaaga gaaaaactgc ctggatctgg
     5641 acgttcttca gtggtagaac cgcttgaaga agtttgttcc tgttttttaa atgaagcttc
     5701 tcgattggca cttaaagtca ctgttgctcc aacttctttt tgtgcttgat tcgctgcttc
     5761 ttctgcagcg cttcgaatcg tgaggccagc tagcacaaaa ataagaatgg ctgtgaacac
     5821 ggcaaacaat aacaatgaac gtccccattt tacttttgta cttttccatg cacgtttcat
     5881 aaaattcata ttttgcatcc ctcctagtag atgcatttga taatagacaa cctatcttaa
     5941 agctagctta aatcttatgc tgtttgcaca ccatcaacat gaggttctgt atatataaaa
     6001 agtcactata gttattgcaa aaaatgtggt aaaatgaaaa aagtatgttt caaaccgacg
     6061 aagaagaaat gaaagaggaa tagcaaaaga aatcctagat gctacagagg gatttttacg
     6121 tgcatagttg ttctaacaat tgctgatact agcgatttag tttatttgtt gtataataat
     6181 ggggaacagc tgaatactta tataagtatt ctggctatag cgtttcgcaa aagatccatt
     6241 ttgttaatga agtatatcca aaataaaatg cccatccaac tgtcagaacg cggatgtgct
     6301 ggtaattttc ctgctgattc tccataagtt gagaaatagt gattttgcat atgcttcatt
     6361 ggaatggctc atatatttag ataaagaaga aaatcgacat ctttaaactt ggatgtcata
     6421 agaagaggtg aagtttgtga gtaccattaa aattatccca ttaggcggcg ttcgtgaaaa
     6481 tggaaaaaac atgtatgtcg cagaagtggg cgaggatatt tttattttag attgtgggct
     6541 aaaatatcct gaaaatgagt tgttggggat cgacgtcgta atccctgatt ttacgtattt
     6601 agaagaaaac attgatcgtg tagctggtgt ctttttaaca catggacatg cagatgcgat
     6661 cggggcactt ccttacttgt tgtcaaaaat ttctgtacca gtattcggga cagagttgac
     6721 gatcgagtta gcaaaattga atgtcagtcg taatgaagca gccaaaggat ttaaggattt
     6781 ccatgtggta gatgagcata cagaaataga ctttggtgaa acagtagtga gctttttccg
     6841 taccactcat acgataccag attcagtggg gatcaatctg aaaacaaaag aaggtagtat
     6901 cgtttatacg ggagatttta aatttgacca aacggctatc ccaatgtatc aaacagatta
     6961 tgcacgattg gcggaaatcg gcaaagaagg agtcttagcc ttactaagtg aatcggcaaa
     7021 tgcagaaaat cctcagcctg tagcttctga agcccaaata gcagatgaag tatttgatac
     7081 gatcaaatat tgggaagggc gtatcatcgt cgcttgtgta gcaagtaatt tgcaacgagt
     7141 gcaacaagta ttggatgcag cagaaaaagc acaacgaaaa gttgtcttga ctggtcaaga
     7201 tttcgaacga attattcgca cagcgatgcg tttagaaaaa cttcaattac caagtgaaga
     7261 tctgcttgtt acgttgaaag acatgaaaaa ctatgatcca gaagaactga tcattttaga
     7321 aactggccga atgggtgaac ccatcaagtc tctgcaaaaa atggcaaacg gtacacaccg
     7381 taatgtcaag attgaagaag gagatttggt ttatatcact actacgccaa gcatcgcgat
     7441 ggaaaccatc gtggcgaaaa cggaagatat catttatcga gctggtggaa cagtgaagtt
     7501 gatctctgag aatatgcgcg tttctggcca tgccaatcct aatgacttgc aattaatgat
     7561 caatttgatg aaaccaacat attttgtgcc agttcaagga gagtatcgtg aattagctgc
     7621 tcatgcagat ttggctcatg cagtagggat gccatacaaa aatatctata ttactggacg
     7681 cggagacgtg ctggaatata agaacaagcg tatgagtgtt gcaggaacta caagtgcaga
     7741 aaatgtaatg attgatggat taggcatagg tgatatcgga aacattgttt tacgtgatcg
     7801 aaaaatcttg tcagaagacg ggattttcgt agcggttgtc actatcagtc ggcgagaaaa
     7861 gaaaatcatt tcaaaacctc aaattacttc acggggattc gtttacgtaa aagctagccg
     7921 tgatctgatg aaagaaagca gcaatatgat tgaaacaatc gttgagaaac atttagaaag
     7981 caatgatttt gagtggagca aattaaagca agacatccga gatcaattag gccgttactt
     8041 atttgaacaa acaaaacgcc gcccagtgat cttgccagtc attatggaag ctactcaacg
     8101 tcgaaataaa aaataaacaa aaagagtaag atgaacctct ctatagagtg ctcgtcttgc
     8161 tctttttttg cgaaaaacaa ataatttttg aatacagcat ccctcgcttt ataataaacg
     8221 atagaataaa gggagtgaat caatgatgag tcaaacaaaa gtcgaacgtt atttagaaga
     8281 acaaaagatc agctatcaac cacttgattt tagtcatgtg gtagaagaat ctttcacaga
     8341 agaactacaa aaaagaggga ttgatccagc tttgatatgt aaaactctcg tagtgaaagg
     8401 aaacaaaact ggggtgatca tcgcattgat tcctttgaac gatcacttag attacaaaaa
     8461 aacgagacaa gtgacgaaag atcgtaaagt tggttttcct ggaatggatt ttgtattaat
     8521 gcatacagga tatccccacg gagcaaacac ccctattgga ataaaactcg cacatcctga
     8581 ttattgtttt ttagcagaca gttctttaaa agaaaaagaa gaagtaattg tgtcaagcgg
     8641 tgaaatcggg agaagccttc ggataaaagt agccgattta gaaaagatcg tccatcctat
     8701 ttacgctgat ttaattagtt aagggacgag aagagaagct aagcagagca gagacctcaa
     8761 acttgagatt atatctgctt ggtacttggt gcatcctgta ttatctgtta caattgcttt
     8821 gtacatactg tactatcaat ccaatgaaaa tatatttagc agctatattt tttcattgat
     8881 gtgcaagtga tgaaatcgtt tttaataaaa aagatacaga gaaaacgcga ggtgtaaaat
     8941 atgaaaatta cgtatcatgg acattcctgt ttaacaattg aaatggaaaa tggccaacgc
     9001 gtgatgattg acccgtttat tacaggaaat ccattgacag acttaactgc agaagaagta
     9061 aagacagatt ggttgctgat tactcatggt catagtgacc acataggaga tatgattcct
     9121 attgcaaaag aaaatcaagc cacaattatc agtattgttg agattgctga ttatgcgaac
     9181 agcagaggag tagattcatt cggtatgaat atcggtggaa aacatgcgtt tccattcggc
     9241 gtggtaaaat tcgtacatgc acagcatagt tcaagctatg aagtagacgg tatcgttcag
     9301 tatatgggcg agcctagcgg aattattatt caagcagaag gaaaaacgat ttatcatgca
     9361 ggggatactg cttatttcag tgatctagga ttgcttgcag aagaattcga tattgatgtc
     9421 gctttcttgc cgattggcga taactatacg atggggccag aagatgcagc aaaggctgcg
     9481 caaacaattc gtgccaagaa agtagttcca atccattaca atactttccc tgtcattgaa
     9541 caagatcccg aatcttttgt agagttactg gaagatagtg aaggaaaaat aatgagcgtc
     9601 tcggaaacgc tggaagtgta ggcagaaaat ggcgacaaaa catgatttga ttttagaata
     9661 tattgaaagt ttacctgttg gcaatcgaat ttctgtaaga agcatcgcaa aagaattgaa
     9721 tgtcagtgaa ggaacagcgt accgtgcaat caaagatgca gaaaatgtcg gactagtttc
     9781 aacaattcaa cgagtaggga ccatccgtat cgaacgcaaa ttaaaaaaac atattgaacg
     9841 tttgacattt ggcgaggtcg tcaggatcat tgaaggggac gttttaggcg gggcggcagg
     9901 attagacaag gtcttgaata aattcgtcat cggcgcgatg actaaacatg ccatggaacg
     9961 ctatattacg ccaggatcac taatgatcgt cggcaaccgg gcggaagtcc aaaaattagc
    10021 attggaagat ggcgccgcag tattgatcac aggaggattc aatacgactg ctgagattgc
    10081 aaagctagct gatgatcttg aaatgccgat cttacgcacc tcttatgata catttacagt
    10141 cgcaaccatg atcaaccgag cgttaagcga ccaattgatc aaaaaagata tcatgcttgt
    10201 cagtgatatc tatatgccag ctgaaaagac tcattatttg cagcagacag atacgatttc
    10261 tgattatcag cgtttgtcag aagaaacgca gcattcgcgt tttccagtcg tcaaccgcca
    10321 tcatcgtttg atggggattg ttacggcaaa agatgtttta gggaaatcgc caaaccagat
    10381 catcgatcgg gtgatgacga aagaaccaat cagtgtaaag aaaacaatga gtattgcctc
    10441 tgttagccat caaatgatct gggatgggct agaagtcatg ccggtcgttt ccgatgactt
    10501 gacattggaa ggcttgatta cccggcaaga tgtaatgaaa gcgatgcagc ttgtccagcg
    10561 acaaccgcag attgcagata cgatttcgga tcagatatct ggaaatatcc atacgattga
    10621 tactgaccgt gaaggaaatc gtttggaaaa ccctaagttc aaattcgtag tagcaccaca
    10681 aatggtcaat ggcgttggaa ccatttcttt tggcgtactt agcgaaatca tttctgatgt
    10741 cgcacaaaaa acaatggtga tgaaccaaaa acgaaatatc ttgattgaac aagtgaacct
    10801 acattatttg cggctgatcc aattggaaag cgaattagac attcgtccac gtgtattgga
    10861 aatcggcaga cgttccgcta aactggatat cgaagtgttt attgaaaaca ccatcgtttc
    10921 caaagcaatc gttgtttgtc aagtaatgga acgttcttag aaagcaggag tgaaatgaca
    10981 gtacagcaag aaatagtaga tacaataaaa aaatacgata aaatcattat ccacaggcat
    11041 ttgcgccctg atcctgatgc attaggatca caagtaggat tagccgaact attacgggaa
    11101 agtttcccag aaaaagaaat acgtcaagta ggtgaaacga ttgaagggct tcgcttttta
    11161 gcagaaatgc aagaagttga agatgtcttc taccaaggag cgctagtcat cgtgactgat
    11221 acagctaatt cacccagaat cagtgatcaa agatatcagt tggcagaaaa actgatcaaa
    11281 atcgaccatc atcccaatga tgacccttat ggagatcttg tgtgggtaaa tacaaaagca
    11341 agtagctgta gtgagatgat tgcagaattt gctttgatgt ttcctgaatc tttaaagatg
    11401 aacgttaagg ctgcacggtt actttatgca ggaatcgtcg gagatacagg gcgctttttg
    11461 tatccctcca cgacgtcccg tacattagag attgcttcga tattgagaaa ttatccattt
    11521 gatgcaagtg ctttgaacag agagattgaa caaatgccga tgaaagtcgc taaactttcc
    11581 ggttatcttt atcaaaatat ccaggtggat gaaaatggtg caggaagaat aatccttccg
    11641 caagaaattc ttgataaata tggaatcgat gattcagaga cagcttcgat cgtttcactt
    11701 cctggagtga ttgacgaagt gctggcttgg gggatttttg ttcagcagcc ttcagggtat
    11761 taccgcgtac gtttacgttc taaggggccg atcatcaatg aattggctaa acgccatcat
    11821 ggaggaggcc atccattagc aagtggtgcg aatgcaaaag atttagcaga agtagaggaa
    11881 atctataaag agatccaaga tatatgccaa tcgtataaga gcagaaaaac agaatgatac
    11941 ttcgttcgaa aacgtgcctt ttgtagtaaa aatcacaaat gtatcttttc aagatcttct
    12001 ttttccgtta caatagtgct cactaaagat tttaaaggag aaagaagatg acattaccaa
    12061 attgcccaga atgtggctca gagtacacat atgaagatcg aggcttgttg atttgcccag
    12121 aatgcgggaa cgaatggaac aaagaagaac aagaaactgt catagaagac ggattagtgg
    12181 tattagatgc aaatggaaac cgtctgttag agggcgatag cgtgacagtg attaaagact
    12241 tgaaagtaaa aggtgcgtca ggtgccatta aacaaggaac aaaagtcaag aacatccgtt
    12301 tagtagaagg cgatcataat atcgattgca aggtagaagg atttggaccg atgaaactga
    12361 aatcggaatt tgtgaaaaag aattaaagaa tattcaaggt cttttcacat tcttttccta
    12421 ctactaatga taaaataaag taaacgaaaa ggagtgaaac gaaaatgtat ggttatggaa
    12481 tgtatggttt tttagatccg acatttcttt tggtcattct tggattagtt atttccggaa
    12541 ttgcgtcagc gtacgtcaat agtactttcc gaaaatacga tcaagtgaga agcaaaaaca
    12601 atgtgactgg tacacaagca gcacaatata tattacaaag tcaacagata aataatgtcg
    12661 gtgttcaaca aattgccgga gatctcacag ataactacaa ttcaggtaat aagatgttga
    12721 gtttgtctca agcaactgca cagtctactt cagttgctgc gataggtgta gcagctcatg
    12781 aatgcggaca tgcggtacaa gatgcttcgg gttatgtccc attgaagatt cgtgcagcac
    12841 ttgttccagc agctaacttt ggttcgatga tttcgttccc cttgattatg gtaggtgtat
    12901 tgcttagctg gaactctacg ttaatcaata tcgggatttt cgctttttct ttagcattac
    12961 ttttccaatt ggtgacatta ccagtcgaat ttaacgcgtc tcggcgagca atccaaattt
    13021 tgagtgaagg cggactactg acggaagaag aagtaccgat ggcaagacac gtattatttg
    13081 cagcagcact gacttatgtt gcagcagcgc tttcgacatt cctgcagctg ttgcgcttga
    13141 tcttattgtt cggcggcaac cgaagagatt aaaataaaga agttgtgaaa tcagcgcttt
    13201 gatctgagct aataccgaga gttagctgat tgaataccat ttatttggaa taagggctct
    13261 ttgtcaataa ggactgatgg gttgtacaaa atcaaatctg ggtcagaatg aacctcattc
    13321 tggctcag
//
LOCUS       DAJSBL010000071        12734 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.073, whole genome shotgun sequence.
ACCESSION   DAJSBL010000071 DAJSBL010000000
VERSION     DAJSBL010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12734
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.073"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(85..264)
                     /gene="rpmF"
                     /locus_tag="ONT85_002435"
     CDS             complement(85..264)
                     /gene="rpmF"
                     /locus_tag="ONT85_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HCR2379384.1"
                     /translation="MAVLARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(322..891)
                     /locus_tag="ONT85_002436"
     CDS             complement(322..891)
                     /locus_tag="ONT85_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HCR2379385.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1077..1979
                     /locus_tag="ONT85_002437"
     CDS             1077..1979
                     /locus_tag="ONT85_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR2379386.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="ONT85_002438"
     CDS             complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="ONT85_002438"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HCR2379387.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="ONT85_002439"
     CDS             complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="ONT85_002439"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HCR2379388.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="ONT85_002440"
     CDS             complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="ONT85_002440"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HCR2379389.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KGQLIYLLGEDNVVFK"
     gene            8547..8732
                     /locus_tag="ONT85_002441"
     CDS             8547..8732
                     /locus_tag="ONT85_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HCR2379390.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(8794..9990)
                     /locus_tag="ONT85_002442"
     CDS             complement(8794..9990)
                     /locus_tag="ONT85_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HCR2379391.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVARAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10160..11596)
                     /locus_tag="ONT85_002443"
     CDS             complement(10160..11596)
                     /locus_tag="ONT85_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HCR2379392.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            11889..12698
                     /locus_tag="ONT85_002444"
     CDS             11889..12698
                     /locus_tag="ONT85_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCR2379393.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
BASE COUNT         3803 a         2675 c         2249 g         4007 t
ORIGIN      
        1 attttttatt taccgactac aaaaaaataa acctcaaggc tccatggtta gccttgaggt
       61 tttacatttt ctaattaaaa tgtattaagc ttcttttgac attacgtcct taccatcata
      121 gtaaccacaa tttgcacata cgtgatggct ttttttcatt tcaccacagt ttggacattc
      181 gtttaaccct ttgattgtca atttgtagtg tgtacgacgt tttgcttttt tagcttttga
      241 tgttcttcta gctagtactg ccattttgct acacctcctt gtaaagtctt gtatctaaat
      301 tagacacaat atattccaat cttacttgtt atcgtcgtct tttgatttag aatcaaaaag
      361 ttcagatagt tttgcaagac gaggatcaat tttattctcg gcttcttcca tttttctgcg
      421 ctggtattct tcttcggaaa caacttccca atcattgcct tttggaagtt ctgttgctgc
      481 ttcctcttct tttgtcaata cccgcattgg aatcgccaat aagatgttgt cttcaatcga
      541 ctctgctaaa ttcaataatg gaccttcaat tagcaaaatc tcatctgcag acatttgttc
      601 atctctactt tggaattgtt cttctgtcat gaatacttcg tctacagaaa aatccatggg
      661 cagttcaaca ggttctagtg aacgtgttga aggaactgtc aaagtcgtct gcacagtgta
      721 atgaacgata tagcctttct tatcaactga gaccaaacct tcgacttcca ctggcgcaac
      781 atcaagtatt aaattgtctc gcttcatgag agcggctttc aagtcgagcg tctcatgaaa
      841 agttaacggt gtctcctggt attttctcaa ttccaataaa gaccatttca tttttcatca
      901 ctcctaagca acaaaagcaa ttatacaggg caaaaagcat gttgtcaatg aaattttctt
      961 gacaccttgc agttcataaa aaaacagaaa aatctttatt agaaagcctc tgtttgctat
     1021 catcctcaga atcatttaca ataatcatta ttccattaaa ggagcggatg atcttcatga
     1081 acacgaaaga tatcgaatac tatgtccaaa tcgtcaaaca aaaaaatttt acgaaagtgg
     1141 ctaattattt ccgtgtgagc caacctacta ttacatatgc attacaacgt cttgaaaaag
     1201 agtttgacac cgtacttatt acaagagatc gatcgcatca cgaattaatc gtcacgcctt
     1261 ctggaaagca gctgtttctc catgcacaaa ccattctcca agagatttct actgcaagag
     1321 ctgaaatcac tcagttgcaa aagaaaaagc ttcgttttgg catgcctcct attatcggca
     1381 atgaatactt tcctaaactg tcaagttact ttatcaagca tgaattgatg aatcaaattg
     1441 agattaccga tggaggctct cgggacttat acgggatgat ccgacaaggg aggatcgatt
     1501 tagctgtatt aggctcaaca cagccaatcg tggatcaata tttagaaaca gagttacttt
     1561 tggacaaacg tttcatgatc gtcgtgccac ctacacatcc tctttccaag caggatactg
     1621 tctctttcaa agatatagca gatgaacagt ttgtactatt gaacgaacat tatgtccacc
     1681 ccgcagcatt cactaagctt tctaaacaaa cacatactca tccaaaagtt gtttatcaaa
     1741 gcaatgactt gacgatttta aagagtatgg tccgagaaaa tgtgggtatc ggttttttgg
     1801 cagagattgc tgtccatcct gaagatcatt tagtttgcct ccctctttca gatgaagttc
     1861 agcctcgctt cttgatttca ttggcaaaac gaacacagca gatcgtcacg cctcttcaag
     1921 aacaagtagt ccaagttatc catgaattcg ttacagcaca aaaaaacagc tcttcgtaaa
     1981 agaagagctg tttttatttt ttattaagct aaattagata gctgttgttt cgccacggta
     2041 aacgatgccg cgacgtgaat caacggtaat caattcacct tctgtaattg ttgttgttgc
     2101 gcctttagca ccaacgatta caggaatatc ttgtgcaatt cctacaactg cagcgtgaga
     2161 agtcaatccg ccttcttcaa cgataacagc agctgctttt tcgattgcag gcatatattc
     2221 tttgtcagtt gatggtacaa ctagaaccat tccgtctttt gcttttttta cagcttcttc
     2281 agcagatgaa gcaataactg catttgcaac gacagatttt tgtccaacgc cttgagcttc
     2341 taataattta gaaccaatca tttgaatttt catcacgtta gttgttccgc gttcaccaac
     2401 tggtacacca gcagtgatca agattaaatc gccttctgat gcgaaaccta attcaacagc
     2461 ttttttagct gctagttcaa acatttcatc agttgttgat ggtttttcag caactgttgg
     2521 gtaaacgccc cagtttaaca tcaaaccacg ttttgtgcgt tcgtcaaaag taacagccaa
     2581 gatatctgca tctggacgat attttgaaat catttttgca gtgtatcctg attcagttgc
     2641 agcaacgatt gttttcacgc ctagattttt tgcagcacga gctacagata aaccgattgt
     2701 ttcagttaca tctgttttat cgaattcgtt caattggaat gtacctaatt ctgataatgc
     2761 attttcagct tcttggtcga tacgtgccat tgttgaaaca gcttcaactg ggtagtcccc
     2821 gtttgcagat tcaccagata gcattgttgc atctgtgcca tcaaatacag cgttagcaac
     2881 gtcagaagct tccgcacgtg ttggacgtgg gttttcttgc attgattcta acatttgtgt
     2941 agcagtgata actgggatac cagcagcatt acattttttg ataatgcgtt tttgaaccat
     3001 tggtactagt tcagctggga tttcaacacc catatctccg cgggcaatca taacaccgtc
     3061 agaaactttg atgatttcgt caatgttgtc gataccttct tgagattcga ttttagggaa
     3121 gatttgaaca tgagtcatgt ctttttcttc taagatttcg cggatatcta gaacgtcttg
     3181 tgctttacgt acaaaacttg cagcgataaa gtcgatatcg ttgtctaaac caaaacgaat
     3241 atcgtcagca tcttttggag taatacctgg caaactaata gaaacgcctg gtgcatttac
     3301 ccctttacgt gatcctagca tacctgcatt tttaacttct acgactaatt cacggtttgc
     3361 ttcgtctttt tcaatgattt gcatatcgat caaaccatca tcaaataaaa tgtgtccgcc
     3421 aacgtgaaca tcgtcaaata atcctgggta agtaaccgcg attttttctt ttgaacctac
     3481 atgttcagga tccatagaga tgcgtgtttg atctcctact tcaaatttga tgtatcctgc
     3541 acgaccaaaa tcagcttctg ttgtgccttg aacagttgta cggatttcag ctccttttgt
     3601 atctaaaaga ataccgacat cttttcctgt ctttttaact gcttcacgga ccatagtcat
     3661 acgtgataat tgttcttcat ggtcaccatg agaaaaatta aagcggaaga catttgcccc
     3721 agattcaatc aattgagaaa taatttctac tgagttactt gctggtccta atgtactaac
     3781 gattttggtt tttttcattt aaaaaactct cccattcaat tttgtttatt taaagctgta
     3841 cacagcttaa atagcttggt ttctttcact cttaaaatga aagtgaatta tttagatcat
     3901 atagtgataa gtctggttta tgtttgttgt tttcaagtgt atcaatgatg tcagacgcaa
     3961 cgatttcgtt gtcatggata ccgacacata atccaccttt tccttcttga agcaattcga
     4021 cagcgtaagc accgaattta cttgccaaca cgcggtcgcg tgcgcttggt gctccaccac
     4081 ggacaacgtg tcccaagata gatacacgtg tatgatagtc accaaattct gaaagtttct
     4141 cagcgaattc atttccgccc attacacctt cagccaagat gatcaagcaa tgtttcttcc
     4201 cgcgatcgcg gccttcttgg atttttttag caactgaagc catatcaaaa tcatgttctg
     4261 ggatgatgat ttcatccgct ccgccagcca cacctgacca taaagcgatg tctcctgcat
     4321 tgcgtcccat tacttcgata acgaatgtac gaacatgaga agtcgctgtg tcacgaatac
     4381 ggtcaattga ttctagtact gtattgattg ctgtatcaaa accgattgtg aaatcagtcc
     4441 caggaatatc attatcgatt gttccaggaa tacctactgc tgggaaaccg cgttttgtca
     4501 aagccattgc gccatgataa gagccgtctc ctccgataac cactagacct tcgattccga
     4561 attttttcaa ttgttcgatt ccttttaatt gaccttcttc agttgcaaat tctggataac
     4621 gtgcagaata caagaatgta ccaccacgtt ggatcttgtc acctacatct gccacatcaa
     4681 gacgacgaat atctccagca actaaaccgg caaaaccata attgatcccg tatacttcca
     4741 gtccttcgta aataccttta cgaacaacgg cacgaacagc tgcattcatt ccaggtgcgt
     4801 ctccgccact ggtcaaaatt ccgatgcgtt tcataataaa cattcacctc attaaaatac
     4861 tttatcctaa gtatgactta ggtttacaac catttttgcg ttaaccacga ctaacattct
     4921 aacattattt ttctcacttg tcttgattta cggataaaaa aatgaaaaaa aatgaaagca
     4981 agtagtattt tttaccgtaa gttatttgaa aaccacatta tcttcgccaa gcagataaat
     5041 aagttgcccc tttatactct tgctgtcatc cacccaataa gcatctgaca gaactacttt
     5101 tcgttgcgtt ttttcgtgat acataactac tggtatatat cctgggtgtt tctgaaaaat
     5161 cacttgtaat tgtttcatta tctcgggatc ttcccgttct tctgtaatac gcaaatagca
     5221 agtttgtgaa ggatactggg cttctaactg gttaggatca gcaaagcttt ctccaattag
     5281 ttgcagttcc tgattatatt tgctgatttc gctccgccct tgaacaaaga tcactcgatc
     5341 aacggcaaac aaattacgat atttacgata aagagttgga aaaactgtga cagaaatttc
     5401 tccactactg tcggttcctt caacaaaggc catcagttct cctttttttg tccgaatttc
     5461 tctaacttcc ttgatcatca ctaataaatt gactggttga ttagggacga tatctgtaat
     5521 cagctgaact tgtttagcta aacgaagacg atcaaaccct tctgcaggat gacctgacaa
     5581 ataaatacct aagtactcct cttccagctt caagcgttct tctagtgtat agtcagcgac
     5641 ttcttcttct tttaatgcca tgattcccag caagtcaaga cttccaccac tgtaaacgat
     5701 attttggatt tttccttcga gatcaagcat caattgtttc ctgttaggag ataattcatc
     5761 aaaaactcct actgctgtca gtgattgcag taactctaat ttcagccaac gcttatcgat
     5821 ccgtatgagg aattgatcca ctgactggaa cgggccattt tgttttcttt cgtttaatat
     5881 atcttctaca aaatcacgcc gaataccttt tatcgctgtc agtccgaatc tgatttcttc
     5941 cttattgacc aactcaaaac tggaattact ttgattaatg gacggcgagg aaagtttgag
     6001 tttattttta cgtgcttctg caatatattc tttcaatttt gatgtgttat gccgaacaga
     6061 attcatcagg ctggtaaaga aagcacctgg atgatgggct tttagatacg ccatttggaa
     6121 tcccacgaat gaataagcaa atgcatgtga ccgattgaat ccatagtttg caaatttttc
     6181 gatatattca tatacggtcg ctgctgtttt ttccgaatat ccctgttgta atgctcctga
     6241 tacaaaatgc gacttttctt catctaatac gtcttttttc tttttactca ctgcccgtcg
     6301 cagaatatct gactgcccta atgtaaatcc agccatagtc gaggcgatct gcatgatttg
     6361 ttcctgatag acgatcactc catatgtgtt tttcaaaatc gggatcagtg aatcatctgg
     6421 gtagcgaatg gcttcttttc ctttcttcct ggcaatgaag gtatctatat tttgcatagg
     6481 gcctggtcga tacaatgcat tgactgcggc aatatcttcg atgttttctg gatctaatct
     6541 tcgtaaaacg ttccggatac cagcagattc aaattgaaat actcctgagg tttcgcctcg
     6601 ttgaaacaag agcagcgttt ttgcatcatt tagcgggatc tcttttaatc ggatctcata
     6661 cccttctact cgcttaacag ctttcaaagt aaaatcaata atcgataagt tgcgtagtcc
     6721 taaaaagtcc attttcaaaa gaccgattgc ttctacctca ttcatcgtat actgggtcag
     6781 aaaagcttca tcagaaccgc gttggagcgg aactagttca agtagattct tatcgctgat
     6841 aactacacct gctgcgtgag tcgaaacatg tcttggcaat ccttctaaaa tcactgcagt
     6901 tttgaataaa agttggtttt tttcgcttgc ctgaactaat tctactaatt gatgcgaagt
     6961 tttataagct tgggtcaatg tgattttcaa ttgattgggt attgcttttg accagcggtt
     7021 tgcttcactt tgcgataaac caaacactct agctgcatct ctaagaacca tttttgctgc
     7081 cattgtgcca aatgttgcga tttgtgccat atgatattgt ccatactttt ctcgaacata
     7141 attcaatact tcttcacgtc gattgtcagg aatatccaaa tcgatatctg gcatcgtaaa
     7201 gcgttcgggg tttaaaaagc gttcgaacag caaatcatat tctaatggat ccacatctgt
     7261 gatttctaac acataggcta ctaatgaacc cgcagcagaa ccacgacctg caccggtaac
     7321 gattttgttt ctatgagcaa aagccattac atcccatacg atcaagaaat aatcatcgaa
     7381 tcccatttga tggatgacag ataactcgta gtccaaacgt tcttggtatt cctttgtata
     7441 gtcagtgatt cggcgttcca cttgcgaata gcataactct cttaaatatt ctccagcttg
     7501 ttttccatta ggaactggat aatgcggaag taatttctga tggaactgga attcaaatga
     7561 aatcgattca gctaaacgct gggcgcctat tacagcttgg gaaagacccg ctttttcgaa
     7621 tcgtttgatc atttcttgtt ctgaaagaag atagacattt gctgttagtg gtaaagatgc
     7681 agataaattt tctaaaggta tttggcttcc ctctttgata tgtttcatca cttcaagtgc
     7741 aaaaagttct tcttgttgta aactagcgat gggatgtaat gcacaaagcg aataattttc
     7801 ttttatcaca tactgatacc attcaggcaa ttgatcaggc gtgttttctg tcaagccgat
     7861 ataaaaattt ctagtaaaaa cattttcaag taaaaactgc caatgaccga aaaagttatc
     7921 ctctttattg aaagcctcag aaacagaatg atttgccgga acaacaaacg ctagatggtc
     7981 ggtttctgtc atttctgcaa gttccaattt ttcttggatc atttttcggc tggaaagttt
     8041 catcagctgc tgataaccta tttcatcttt tgcgtacgca agcaagccat aatttttttc
     8101 ttcataagta taactaagat gtaatccaat gattggctgt actccttctt ctacacaaat
     8161 acgatagaat tcaggtgcac cgtataagac atctttatcc gttatggcta atgaagtgta
     8221 gccttgtttt tttgcttctt gtacatattc tcgaatacgt atcgtacttt gtaaaagaga
     8281 ataagaagta attgtataaa gttgtgggaa aaacatcttg cattccgcct tttttttcgt
     8341 taaaattcac tagcgttaaa aggtttgatc cttttacttt cattaagata gcaggcgctg
     8401 aagttgatta ttccacttca tttgcttgct tgtaatctgt aggaatgagt tctttcgcaa
     8461 gaactcaaat tttattgaga tttggtctcg tttctttaca ttcaaagaaa atctggtaaa
     8521 attaagatag aaaagaggtg ttctttatgc gatatatcgt tacattattc tgggctgtcg
     8581 ttctaggtca agtagtcggt tatattggtg ctgcattaac aagcggaaca tatgatttta
     8641 ctttgacaac aatcatttca ttcattgcgg gtgtcattat ccttttgatt ggtgcagttg
     8701 cgccaagaaa agaaacctct gcccattcgt aaaaaaacag ccaagagctt gagatttctc
     8761 aagctctttt ttattttcta ctcctcacca ccattataat ctgctgcgaa gaactttacg
     8821 aaagttgaat aaagaaagaa gaatatttga cagaacaaac aaacttgtca caataaaaac
     8881 agcactgtaa ttataagcac gcgcgactgt cgatcctagc atgggaccta aaacttgtcc
     8941 aaagttgcta aacatctgat tgtagctata gattcggctg actccttctt gaggcgtaat
     9001 cttactgatc agtgtattga cagaaggcat caaagcgcca gttgaaaagc ctaaaataaa
     9061 gcgaagtata cctaattgga gcggtgtagt gacgaatgcc ataggtaaat aacagcaaaa
     9121 tgataaaatg agtcctccaa ttagaacttt ttgattccca atcttatccc ctagttttcc
     9181 tagtttcggt gaagaaaaga cagcggaaac accagctatc gaaacaatca agccgctgac
     9241 aaaaaggata ttttctgtac ttccgctcaa ttcacgaata tataaggtca aaatagggct
     9301 gatcgtagtg attcctatct gtaaaatcaa ggtcgtaatg aagagaccga tcaaaataga
     9361 gagatgatcc atttttgaaa aaatttcttt ggtgcttatc agatcttttt tctcgattgg
     9421 atggaaatct tctttgacta agaaaatggt caataccgtt gtgatcatca acaaagcccc
     9481 tgtgatcaag aatacatttt ccataccaaa ccactgagcc agtgcaccgc ccattgaagg
     9541 tccgatcaga tttccagcaa tcgctccggt agacaatgtt cctaacgccc atccgctctt
     9601 ttctctaggt gcctgtgaag caatcatcgc tgtggcattt ggaatatatc cagacaaaat
     9661 cccattccaa aaccgcatga tcaaaagcca atacgcgttt ggtacaaaag ccaaagatcc
     9721 cattgttaat gtcatccctg ctgctgcacg gatcatcatg agttttcggc cttttcgatc
     9781 agctagattt ccccagattg gtgcaaccac ggcagatgca aaagcagtca cagagattgc
     9841 tagtcccgaa aacaattcga tttgatcttt tggtgttcct agttgctcaa cataaactgg
     9901 tatgaatggc ataactaaac taatacttgc accagtgaaa aaacagccaa tccacgcaat
     9961 cactaaattt tttctccaat taatctccat ctatcatccc tctttcgtct cttttatcat
    10021 acctattttc gaataaacaa gcaacgatct cgccagtaaa aagagaaaaa tcccgcaaag
    10081 gtcattttat atgtaacgtt ctttttatgt aaaacaaaaa gagatcagaa caaatactgt
    10141 tccaatccct tctctttagt taaattgttg ctagaatgat gaagttgata atgaataaaa
    10201 tatcaacgat ccacaaagca acagaaactt gattgtattt tcctttgatt actttcacaa
    10261 ttgtatagaa aataaatcct gctgcaatcc cataggaaat gctatagcac aatcccataa
    10321 agatcgatgc aaagaaggcc ggtaaagctt cttccatatc tagccatttg atgtctgcaa
    10381 aagaagccat catcattact ccaactaaaa tcaatgctgg cgcagttgct tgatttggta
    10441 caatggcgat cagtggagag aataaactac ttaacgcgaa tagcactgcc actactactg
    10501 aagtcaaacc agtacgtcca ccggccccga tcccagctgc agattcgaca taagttgttg
    10561 tattcgatgt accaaagata gcccccacag aagtagcgat cgcatcggca aatagtgctt
    10621 tatccatttt tgttttgaac ccacgaccat cttccaacgc gatctcgtct tcttttgaga
    10681 aaatacctgt gcggcgtcct gtaccgataa atgttccgat cgtatcgaaa gtatcagata
    10741 aactgaatgc gatgatcgtc atcaatactt gaggaatctt ggaagaatca ctgaataaag
    10801 attgcatgcc ttcagaacca aatgctgcac cgaaagttgt tcctaattct ttaaatgaat
    10861 tacctaacga attcgtttgc caatcaatgg aacttaaatc aacaacaccc ataaaaatac
    10921 caaggactgt ggtcgctacg ataccgataa gaatagcccc acggacattt tttacaacta
    10981 aaatggctgt cagtaccaaa ccaatcacag ccaataagat aggagcgttg tcaaagttgg
    11041 ctaacgcagg gacgattcca ccatttatcg taacatttgt cgcttttccg ccttcaacga
    11101 cagagctcga aatagctgac tggtctgctg aaaaagataa gaatcctgca tttttgattc
    11161 cgacatacgc aacgaaaata ccgatcccgc caccaatcgc atgttgaaga ctttcgggaa
    11221 tcgcccgaat aatcattttt cgaatatttg tgacagtaat aaaaatattg attaatccgc
    11281 aaataaacac cattgccaag gcttgttgcc aagaataacc aagcccaaaa acgaccgtaa
    11341 aagtgaaaaa tgcgttcaat cccataccag gtgcctgagc atatggtaca tttgcgaaca
    11401 atcccattac cagcgtacca ataatcgatg caatgattgt cgctagaaat actgcttgga
    11461 agggcatacc tgatgctgac aaaatcgtcg gatttacgaa taagatataa ctcatggcaa
    11521 agaatgtcgt tactcccgcc attatttcag ttgatacatt tgtattgttt ttctttaaac
    11581 cgaaaaactt ttccataaaa agttccactc cctgaatttt tttctgatgg aaaactataa
    11641 taataaaaaa taagtaaaat agggcaaatc catcgaacaa tttcaaatta tatagacgtt
    11701 tcattcgctt ttcaacgttt ttttaacaaa aaaagcgaaa ataatactta acgttcgtgt
    11761 ttagcttatg tttagctatt tatatagcca taactgtagt ttttgttagg aatagctgat
    11821 tgctcttgtt aattacctaa aatatgctag actaaaacaa atgctagttg aggaaggaag
    11881 gacccttatt gaataaaaaa ttaattgcca tcgatctgga cggaactacc ctcaatcaag
    11941 attcgatgat tacatctaaa acaaaagaga ctctaaaaaa agcaatcaat gccggtcatc
    12001 atgtcagtat tgctacagga cgcccattcc gaatgagcca tcagttttac caacaattag
    12061 aattaacaac accgatggtt aattttaacg gcgcattagt acacatccct aatcagcact
    12121 gggatggtga aagagaaacg ttgatcaatc gtgaaatcgc ttttgaaatc ttatcgcaaa
    12181 aaaagcagct aaatttagat tttatcgctg ctgaaaatcg tgatactttt tttattgaca
    12241 gttttgattt ctttgatgaa aaaatttttg cttcttctcg tccaggagag aagaatttgc
    12301 tttctcctaa aaatttgacg acaaatccta catctttgct tgtccgaaca gacaaacgtt
    12361 ttgcaggagc tgtatctgct gaattaacac gtcaatttgg cagttatgtc gatgttcgga
    12421 catggggtgg acctactgcc atcttagaga ttgtttcaaa aggaatccaa aaagcaaaag
    12481 gcgtgcaaga gattgctaac tacctgtcta tcgatcaaaa agacgtcatt gcctttggtg
    12541 acgagcataa tgatttggaa ttgttggatt atgcaggttg gggagtagct atggctaacg
    12601 gtactgacca actcaaaggc attgcaaatg atgtcactcc tttgagcaat caggaagacg
    12661 gtttagctgt ttatctggaa aaactactta aactataata gagaattaga ttccacaaaa
    12721 aagaggttgt gaca
//
LOCUS       DAJSBL010000072        12307 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.074, whole genome shotgun sequence.
ACCESSION   DAJSBL010000072 DAJSBL010000000
VERSION     DAJSBL010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12307
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.074"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            287..694
                     /locus_tag="ONT85_002445"
     CDS             287..694
                     /locus_tag="ONT85_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2379394.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
     gene            708..1109
                     /locus_tag="ONT85_002446"
     CDS             708..1109
                     /locus_tag="ONT85_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2379395.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
     gene            1111..1482
                     /locus_tag="ONT85_002447"
     CDS             1111..1482
                     /locus_tag="ONT85_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2379396.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKIINKLT"
     gene            complement(1518..1817)
                     /locus_tag="ONT85_002448"
     CDS             complement(1518..1817)
                     /locus_tag="ONT85_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2379397.1"
                     /translation="MLFNETQLWFKFDPTNRFIKDFYKVWNSEVFFLAIEDSLLINLY
                     YSNKNYFKIPAAKTRMKKGVYFLFDIVTDVPDARSDHRRYDYIKYTFVDPERYKD"
     gene            complement(1823..2053)
                     /locus_tag="ONT85_002449"
     CDS             complement(1823..2053)
                     /locus_tag="ONT85_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2379398.1"
                     /translation="MMEDFIRKNISDEYADFYEQSSKKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETNFKNRSE"
     gene            2670..3332
                     /locus_tag="ONT85_002450"
     CDS             2670..3332
                     /locus_tag="ONT85_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2379399.1"
                     /translation="MKQNDGRIIWKNQSELKLILTINEFIEKHGITSSRQYQKKLSEN
                     PNSAPSMWFINKKYGSWENLLISIGRENTGYGKWARMSEQELLEIVEAFIKCEKITSQ
                     RMYEQKSVGKNIPSLSTIKKMLGDIRPLFKEKNDGSRFTDFELLLELKNEIIRLKLQD
                     DLSMTKFRKLVQSPKLPSVDTIMKRTNKNWEELMAEIGFDYRRIKIYKQRNNLSKTKK
                     TK"
     gene            3411..6636
                     /gene="fss3"
                     /locus_tag="ONT85_002451"
                     /pseudo
     CDS             3411..6636
                     /gene="fss3"
                     /locus_tag="ONT85_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286924.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
     gene            6750..7064
                     /locus_tag="ONT85_002452"
     CDS             6750..7064
                     /locus_tag="ONT85_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HCR2379400.1"
                     /translation="MELKFVVPDMAETFGKIRYAGEGEVLTEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            7077..7451
                     /locus_tag="ONT85_002453"
     CDS             7077..7451
                     /locus_tag="ONT85_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HCR2379401.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTVSNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIMDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPDQGNSTNQNEGKK"
     gene            7461..7805
                     /locus_tag="ONT85_002454"
     CDS             7461..7805
                     /locus_tag="ONT85_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HCR2379402.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLTKALTYDEKMFLIMHQGLL
                     EVGNDAEEDFDVLNWLGEEESFFMVIEVNESLCREAETVLEEIGVEMPDAIEVFLKQL
                     VETKQLPIAVND"
     gene            7876..9228
                     /locus_tag="ONT85_002455"
     CDS             7876..9228
                     /locus_tag="ONT85_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HCR2379403.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQELRQFDI
                     SKLSLSTIEIVRSIKDLIYLLGSLVLSGSTMLLYIHFFQDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTKETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDIIANRIGIDEVVAESGALRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFVLDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMTRSKAMKEMPNYKTGENYAFLGLPPNFLIFDEYVVYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVDKKFFMKRIKGRGYVDTGGSVISEFYTPLVPKGYDFLREISDIGGLNVNTEG
                     "
     gene            9288..10430
                     /locus_tag="ONT85_002456"
     CDS             9288..10430
                     /locus_tag="ONT85_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HCR2379404.1"
                     /translation="MTFLDLFAGIGGFCLGMEQAGHQCIGFCEIDDFARQSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQGFSDTRGTLFFEIARF
                     AAILQPKFLFLENVRGLLNHEGGATFETILRTLDGLGYDVEWQVLNSKAYVPQNRERI
                     FLIGHSRDACTEQVFPIIGSSPTSDQNIRNLLNINPSNRGMGGQVYGSDDVAPTLTGD
                     EGIKIALPVDDGISVAGMLLGNFEQGNRVYEITGTAPTLSTKQGGTKIMLRKNETEYQ
                     EVKPGNSVNLAFPNSTSRRGRLGKQSVHTLLTGDQQAVVTDQYQIRKLTPRECWRLQG
                     FPDWAFDRASQVNSDSQLYKQAGNSVTVPVIFDIARRLKEVKTDDL"
     gene            10455..10709
                     /locus_tag="ONT85_002457"
     CDS             10455..10709
                     /locus_tag="ONT85_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2379405.1"
                     /translation="MIGKIQSDPELEEELYRLNFDIRKNGVKVPDDPYVIDEETDARM
                     ELNQVISELERIADFAKEPDIRQYLFEIKAELEIEGITAE"
     gene            10965..12149
                     /locus_tag="ONT85_002458"
     CDS             10965..12149
                     /locus_tag="ONT85_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HCR2379406.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATTCGLSRPYLNQIENGGV
                     TASTKTMRKIFDQLESFNPELPLTLLFDYVRIRFPTTDARKIIQEILHLKFDYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPDLTKKCQKEECISLFRTFKSYRSGELLKPDDKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAYHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNQKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNQTTYIQDMVNNTRLSDRHKKILEQQSMAIENL
                     II"
     gene            12146..12283
                     /locus_tag="ONT85_002459"
     CDS             12146..12283
                     /locus_tag="ONT85_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HCR2379407.1"
                     /translation="MKRGIVMEIVKDILLVFGGSMLGVTVMCLMHASAAADRAMEKKG
                     K"
BASE COUNT         4168 a         2017 c         2646 g         3476 t
ORIGIN      
        1 tgaggggaag tacatatagt tgttgatata gttgctaata ttgttagcta atttgttagt
       61 aaaactgtat acgaatttag atagtaaaaa aacagtttgt tccttatata gaggcacttt
      121 tactactgat taaatgtcag atatgcttac aaatatactt ataaatatgc ttacaaaatt
      181 agctaatatt tccgttgaca aagctttgtt actattgtta aactaaattg actagcaaaa
      241 gtgaaacata aatttattaa ataaatgtaa aaattaggag aaatcaatgt cttatagagt
      301 acaattaata atttcagaag atgtagaaag tcagcaattt ggcactaatg ttattaataa
      361 agtaattaat ccagctcttt ctattaatgc gcctttaatt cctactgctt tatcattttc
      421 tgtaacagct gtggtgtctg agatagagga tacagaaaag ataaaaatag tagaaattga
      481 agttttaaac aagaatgaaa aacaaatttt ttctacgggt gaagtatcgg taagcttgcc
      541 tcctcaagtt aacgatataa actttaatat aaacgccaga aatgtcttgg ttgaagaagc
      601 aggagagcat tatgctgttg ttaaatttaa tggaactgag attggaaggc aaatctttga
      661 tatcaaggtc aacaaaccag tggaaaaaaa ttaaggagac gatacggatg cttgatatag
      721 taccatcaaa tacacatcgg agtggtagct taataaattt tgctgcgatt tttgcttgcg
      781 catcggtaat tctagcatct ccagttgttg tggatgcaag ttctactccg aaaactaaaa
      841 atgataatca acttgtaatt actacaaaaa atgagattaa cacaatttca agtaatgatg
      901 gcaaaatttt tgatgtgata aatacggttt taaaaagaaa agctgaaatc aatataaaat
      961 atgatgaaga tttaaacttg tatttctttg ttatcaaaac aacatctgaa ttgttttcct
     1021 cagattacga tgttttggat actctcgata atgtattatc tgactataaa tatatgggga
     1081 aatctgtagt agcaactttg ggggaataaa atgtttgact ttaatgaata tttatctatt
     1141 tccaaacaaa ttgaatcaga tgataaatat aattctaaag aatcttatag aagaactgct
     1201 gtttcaagag catactacag tgcttttaaa aaatctgatg agtatttgaa agaaaattat
     1261 gatattattt ataatggaag tagcggaaaa ggaagccacc aaaccgtttg gaatttgttt
     1321 tcaacagtta aggaattgaa taccttagga atacataata gtgggtatag aatgttagag
     1381 aaaagaaaat gtgctgacta tattcctaat gaaaccatta cgaaaacaga tatggcgctg
     1441 atgaatcgag aagcagaaaa aataataaac aaactcacct aatgaatttc aagcgagttt
     1501 gtttgttatt tttttattta atctttgtat ctttctggat caacgaaagt atactttata
     1561 tagtcataac gccgatgatc gctacgtgcg tccggcacgt cagtcacgat atcaaacaaa
     1621 aagtatacac ccttcttcat tctagttttc gcagcaggaa ttttgaaata gttcttatta
     1681 gaatagtaga gattgattaa taagctatct tcgattgcta aaaagaaaac ttctgaattc
     1741 catactttat aaaaatcttt gataaatcta ttcgtaggat cgaatttaaa ccataattgt
     1801 gtctcattaa aaagcataac cattactccg atctgttttt aaaattagtt tctacctcta
     1861 atatatatcg agtttttatt ttgccttcag agaatactgt ttcttttttt gcagttacag
     1921 gttgtttatt ttcggaaaaa gctaatatag ctaaaattga aacatccatc tggaatttat
     1981 cttttttgct gctttgctca taaaagtctg catattcatc actgatattt tttctaataa
     2041 agtcttccat cataaaaatc acctcaaaac gattatacga acttacgttc tcgttgtaaa
     2101 gcgatatttg aggggcaaaa agggggcaaa aatacgttta tctttgatat atctcagatt
     2161 gaacatagtg tttattatat gtcggtattg ttgatttata gctgttttga gggtattaga
     2221 tacataatag aatatagtgg ttttgtttac gtgcattaca cggaaaagct gctcgtatca
     2281 aagaaatcag aagataaaca cggaaaactt ccttggaaat ctgaaatttg tgaaaagtcc
     2341 ttgtatatca agggcttttt cttttttctc tatcacagtt ttaagccatt tttggctgtt
     2401 ttggaccatt tttcaatcat attcccttaa aaattgtcct agattgtccg acattgacta
     2461 tcagtgtcgt caaaataggg caaaatttag tagtcagctt gacgacaaat ataatacgaa
     2521 aaataccaaa acatttaccg aatttagcaa cttttgaacc caagtttctc aaagggtcag
     2581 ctattagcaa aaaagacaaa acatgtactt ttctactaaa aagttgatgt gataggcttc
     2641 tatctaaata actattagga gtttttatcg tgaagcaaaa tgatggacgt atcatttgga
     2701 aaaatcaaag tgagttaaaa ctgattttaa caattaatga attcatcgaa aaacacggaa
     2761 ttacatctag ccgtcaatac cagaaaaagc tatcagaaaa tccaaattct gcaccaagta
     2821 tgtggttcat taacaaaaaa tatggatcat gggagaattt attgattagt ataggacgag
     2881 aaaatactgg gtatggaaaa tgggctagaa tgtcagaaca agaattattg gaaatagtag
     2941 aagcctttat taagtgcgag aagataacct ctcaacgaat gtacgaacaa aaatcagtag
     3001 ggaagaatat tccttcactt agtaccatta aaaagatgtt aggagatata cgtccacttt
     3061 ttaaagaaaa aaatgatgga tctcgattta ctgatttcga actgctgtta gaactaaaaa
     3121 atgaaataat ccgattaaaa ctacaggatg atttatcaat gacaaagttt cgaaaactag
     3181 ttcaatcccc aaaactacca tcagttgata caattatgaa aagaacgaac aaaaattggg
     3241 aggaattgat ggcagagatt ggatttgatt atcgaagaat caaaatttat aagcagagaa
     3301 acaacctatc caagacaaag aaaactaaat agtatcactc taaatcagac gttcaaaatt
     3361 aaaatttgga cgtctttttt attacccaaa aacgaaagga gatttatttt atgaaaaagc
     3421 caagatttaa gaattggcgt ttgctcgcaa cactgagtct attatgccaa acaattggtg
     3481 gatcgcttgg cccaacgatt gcctttgctg atgaaattac tcatccacaa acggtaactg
     3541 tggaattgga tttggctcac cagtatgctg tcgaaggtac atttagcgat ggtcgtccca
     3601 tgtcagaagt taccgttccg cactatgcag tttacaatgg tgtgaagcaa gatattttct
     3661 gtattgagcc aggggtgccg atttacaatg agttcactcc tggttatgag aaaaatccat
     3721 tgcctgatat gtcggagaaa gcgaaattag tttccgtttt atggaaggaa gctggtacgg
     3781 atgtggatac acatattgtg gcccagaaga tgatttggca agaagttaat ggctacgctt
     3841 cactcaatta aacgttctga tggtagtgca gtaaatatcg cagccattga gtcaaaaatc
     3901 aatcaagcca ttgcggatta tcaaaagaaa ccaagcttcc ataactccac agtgaaaacc
     3961 gtgttaggtc aatcgactac tgtgacagat acgaacggct tgaatttatc ggagtttgat
     4021 gaagttgtcg agaatatcgc aaatattgat tatcgtgtga acggcaatca actagtgatt
     4081 actccaaatg ccaattcaaa agagagtggt gaattaaccc taaagaagtc agctaataca
     4141 ggaacaccgg tagcttataa aatggctggt caacaaactt tgatggcggg ggcaattgat
     4201 aagcccaata cctacacaat caaaattgat gtggaaactg agggttcttt gaagattaaa
     4261 aaagtcgata aagaaacagg aaatgttgta ccgggaaccg ttttccattt agactttgga
     4321 aaaacattac ctgcaaaaga cgtgaccacc gataaagaag gcattgcgac attggatgag
     4381 attcctcatg gcgcgaaagt aaccattact gaaaaatcag tgccagctcc ttatacgatt
     4441 gacactacac caatcaccgc aaccattaaa tctggtgaga ctatttctgt gacttcaaaa
     4501 aatactcgcg aaaaaggtca aatcattttg aataaaatgg gggtggaaac aggaactgat
     4561 ctttggaacg acaaatactc attagccgga aatacgtttg ccattcgcaa agacagtcca
     4621 accggtgaaa ttgtccaaga aatgaccacg gatgaaaaag gtcatgcgga aacaccaaaa
     4681 gagattgcca atgcgttgga attgggaacc tattatgtga ccgaaaccaa agctagtaat
     4741 ggctttgtga atacctttaa accagtaaaa gtcgaattga actatgtcaa tcaaaccgtg
     4801 gctcttgtta ccagtaacgt aaaagggcaa aaccaagaaa tcactggtga aaccactttg
     4861 acaaaagaag acaaagatac cggtaatgaa actcaaggta aagctgagtt taaaggagct
     4921 gaatatactc tctttactgc aaaagatggt caagcggtca aatggagtga ggcttttaaa
     4981 ccagagttag tgaagggaac gaaagcttct gatgaaacgg tgactttgac tttagatgaa
     5041 aagaatcaag ttgctgttaa acacttagcg attaatgagt attactggca agaaaccaaa
     5101 gcacctgaag gatatacctt ggatgaaaca aagtaccctg tttccatcaa aaaggtggat
     5161 gataacgaaa aaaatgccgt gattacccga gatgttacgg caaaagaaca agtcattcgc
     5221 tttggctttg atttctttaa atttgctgga tcagctgctg ggactgccga aactggcttc
     5281 aatgatctga cctttaaagt atcgccattg gaaggaacca gtgagattac aggtgccgaa
     5341 gatgaagcaa ttacagctta taacgagcaa ctaggttttg atggctatgg caagtttgaa
     5401 aatcttcctt atggggatta tttacttgaa gaagtagagg ctccagaagg atttcaaaaa
     5461 attacgccat tagaaatccg ttctactttt aaagaaaata aagcggatta cactaagagt
     5521 gaatacattt ttacgattac cgaaaaagga caaaaacaac caattaaaac ggtgaccgtt
     5581 ccttacgaga aactgacgaa taaagcattt tctgttagct tgaatcgttt gatgctctat
     5641 gatttacctg aagaagaaga tagcttaact tctcttggga cttggaaaga cggaggcaaa
     5701 gaattgacag accttgattc taccgagcta gttgataaat tgagctataa cttgcatgaa
     5761 atcaaagaag actggtatgt ggtagcgcaa gccattgatg tggaagcgac aaaagctgcc
     5821 caagaaaaag atgaaaaagc caaaccagtg gtaattgccg aaacaaccgc aacgttggcg
     5881 aataaagaga aaacaggaac ttggaaaatt ttgcataaat taaccgcaga acaagttttg
     5941 gataaaacca tcgtcttatt caactatgtg tatgaaaaca agaaacccta tgaagcaggt
     6001 gatgatccag tagcgaaaga tgctagcttg aacaatcaag cccaaactgt caattgtacg
     6061 gtggaacgcc atgtttccat ccaaacaaaa gcccacctag aagatggttc gcaaaccttt
     6121 actcatgggg atgtggtgga tatgttcgat gatgtgtcga ttacccatga tgtactggat
     6181 ggctcaaaag aagcttttga aacaattctg tatgcattac ttccggataa tacgaccaaa
     6241 gaaatttgga aatctggcaa aattgattat gaagtgaatg acaaagaatt cactaaaacc
     6301 gtacttgcgg aaaaagtgga cacaagtaaa tatccagaag gtacaagttt taccttcaaa
     6361 gaaattaact atggcaaaga cggaaacatc aatgggaaac acaatgagga tctaaaagaa
     6421 aaaactcaaa ctttaacacc taaaaaagtt ccgacaacac caagtgcacc agaacaaccg
     6481 gaaataccaa ctgttccaag tgactctcaa gaatctagtc ctacagtaaa gacattccca
     6541 caaacagggg agaaaaattc taatgttctg ctgtttattg ggtttacctt gatctttgcg
     6601 actgctgggt attatttctg gaatcgccga aactaaggtg atgtgatgaa acaactaaat
     6661 aggaaaaacc aacgtgcgcg accgcctcct aaaaaaagtg gtcgcgctat ttttgtatcc
     6721 aaaactaaaa atgaaaagag gaaaagaaga tggaattgaa atttgttgtg ccagatatgg
     6781 ccgaaacctt tggaaagata aggtatgccg gcgaaggcga agttttaact gaaggatatg
     6841 ggcggaatac tacagtaatt ggtcgtagtt atcatttgta ttccagtaag caacgagccg
     6901 atgatattga agtggtggta gctgcggaag ccggcgaaaa ggattttgat caagatcaac
     6961 cactaaaagc cgtgaatccc catttggttg ccaaaggcta tgagattgag aatcgtgggt
     7021 ttaccgacta cgtgttgtat gtcgatgatt tagtgaaagc ataggaggaa aagaagatga
     7081 gattaacaga aggtattgtt gtagattcag gactaacgtt tgggaaatta cgattttccg
     7141 cattacgccg agaagtacgg aagcaaaatg aggatggaac ggttagtaac gaggtaaaag
     7201 aacggactta taatttgaaa tcctctgcgc aaggtcgaat gatccaagtt agtatcccag
     7261 caaatgtacc cttacgcgag tttgcctatg acgcagaggt agagttggtc aatccaatca
     7321 tggatacggt cgccaattat gttttccgag aaggaactac tgtcaattgg ttcattaagg
     7381 ccgatgattt agtcttgaaa cgacaaccag atcagggaaa ttctacaaac caaaatgaag
     7441 gaaagaaata ggtgaagcgt atggaaatta atgttgaaca tattttagat tgtctcgatc
     7501 aatatggaaa aggtgagcta acggaggaac aactcactaa agcattaacc tatgatgaaa
     7561 aaatgtttct gattatgcat caagggttac tggaagtagg taacgatgcc gaagaagatt
     7621 ttgatgtact gaattggtta ggggaagaag agtccttctt tatggttatc gaagtaaatg
     7681 aaagtctatg tcgagaagcc gaaaccgtat tggaagaaat tggtgtggaa atgccggatg
     7741 ccattgaagt ttttttaaag caactagtgg aaacgaaaca actccctata gcagtaaatg
     7801 actagcataa ctgcttgaaa ttggatcaat gaaatcctat gaagcttttt tcgattggag
     7861 gtggaaacca taaaaatgta caaagggcat cgtatacggg caggagatca gcacttggtt
     7921 tatcactttg ttcttggctg gctactcgct ctgttcatcg gttggatgag cgtcttttat
     7981 tttcaagagc ttagacaatt tgatatttct aagctatcac tttctactat agaaatcgtt
     8041 cgttctataa aagatttaat ctatttgcta ggaagtctcg ttctttcagg aagcacgatg
     8101 ttgctttata tacacttttt tcaggatcat tggcgcagtt tgtggcatcg acaaaagcta
     8161 gcccggatga ttctagaaaa tcattggtac gaagtaaaac aaactcagag tgaaggattt
     8221 ttcaaagacc tcaatagtag tcgaaccaaa gagacaatca gttacttccc caaaatctat
     8281 taccgtatga aggatggttt actttctatt cgcgttcaaa tttcactggg aaaatatcaa
     8341 gatcagcttt taaagctgga aaagaagtta gaaagtggtc tatattgtga actagtagaa
     8401 aaggaactca aagactccta tgtggagtac actttactgt atgatataat tgccaatcga
     8461 attgggatag acgaagtagt ggcagaaagt ggggctttgc gattgatgaa aaatcaagta
     8521 tgggcttatg attctttacc tcatatgtta atcgctggcg gcacaggtgg tgggaagact
     8581 tactttttac tcactatcat cgaggcttta ttaaaatctg atgccgagct ttttgttctt
     8641 gatcccaaaa atgcggactt agccgattta ggtacggtaa tgcctcatgt ctattcccaa
     8701 aaggaggaaa tttctgcttg tgtggaagat ttctatgagc gcatgatgac tcgtagcaag
     8761 gcaatgaaag aaatgcccaa ctacaaaaca ggagagaatt atgcgtttct tggacttcca
     8821 ccaaactttt taatctttga tgaatacgtg gtttatatgg aaatgttaac gacgaaggaa
     8881 agtgcggtga ttttgaataa gctcaaacaa atcgtgatgt taggtcgtca atctggtttc
     8941 tttctgattc tagcttgtca aagaccggat gcgaaatatt taggagacgg gattcgcgat
     9001 caatttaatt tccgggtggc tttgggtcgt atgagtgaac tgggatattc catgatgttt
     9061 ggcgaggttg ataaaaaatt ctttatgaag cgtatcaaag gtcgaggcta tgtagatacg
     9121 ggagggagtg tgattagtga gttttatacg ccacttgtac caaaaggata tgatttctta
     9181 agggaaatta gcgacattgg tggattaaac gttaatacgg aaggataaaa taatagtatg
     9241 tagcctagga gtgtcaagaa tcctgggctt tttttacgag gtgatttatg acatttttag
     9301 atttattcgc tgggattggt ggcttttgct taggtatgga acaagcgggt caccagtgta
     9361 tcggcttttg tgaaattgat gattttgctc gccagagtta taaggcaatc catgatacaa
     9421 gtaaggaggt ggaaatgcat gacatcacaa gtgtatcaga cgagtttatt caatctctcg
     9481 gaccagtgga tatcctttgc ggtggatttc cgtgccaagc tttttcaatt gcgggaaagc
     9541 ggcaaggatt ttccgatact cgaggtactt tattctttga aatcgctcgg ttcgccgcta
     9601 ttctccaacc taagttttta ttccttgaga acgtccgggg attactcaat cacgaaggag
     9661 gggctacgtt cgagacgatc ctccgaacgc tggatggatt ggggtatgat gtggaatggc
     9721 aagtgcttaa ctcaaaggcc tacgttcccc aaaaccgtga gcggatcttc cttatcggac
     9781 attctcgaga cgcatgtacc gaacaagtat ttcctatcat tggatcgtct ccaacatctg
     9841 atcaaaacat cagaaactta ttaaatatca acccttccaa tcgaggaatg gggggacaag
     9901 tctatgggtc agatgacgta gcgcctacct tgaccggtga tgaaggaatc aaaatcgcat
     9961 tgccggtaga tgatggaatt tctgtagcgg gtatgttact gggaaatttt gaacaaggca
    10021 atcgggttta tgagataact ggaacagcgc ctactttgtc aacgaaacaa ggcggaacga
    10081 agatcatgct tcggaaaaac gaaacagaat atcaagaggt gaagccaggg aacagtgtga
    10141 acctggcttt tcctaattcc acaagtcgtc gaggccgtct gggaaaacaa agtgtgcata
    10201 ccttattaac aggagaccaa caagcagtcg tcacggatca gtatcaaatt cgaaagttaa
    10261 cacctaggga gtgctggcgg cttcagggct ttccggactg ggcttttgat cgagcttctc
    10321 aagtcaattc agatagtcag ttgtataagc aggctggaaa ttctgtaaca gttccggtca
    10381 tttttgatat tgctagaaga ttaaaggagg taaaaacaga tgatttatga tgagttgatt
    10441 ggggaatcta ctgggtgatt ggcaagattc agtcagatcc ggaattggaa gaagaactgt
    10501 accggctcaa ttttgatatt cgcaaaaacg gggtgaaagt ccctgatgat ccgtatgtaa
    10561 ttgatgagga aacggatgct cgaatggagc tgaatcaagt catcagtgaa ttggaacgaa
    10621 ttgcggactt tgctaaagaa cctgatatcc gccaatactt gtttgagata aaagcagagt
    10681 tggaaattga gggtattacc gcggagtaac ggccaaataa accactgata ggcactgacc
    10741 gcgggggatt tgcgaagcag aaatcctccg tggtgctgtg cctgtttatg gacggcgtta
    10801 gccggccatg atgtctcacc ccccgtatct aacagggggg tacaaaaaca caaaaaataa
    10861 gcaagcaacg ataaggaatg gctgtcctat caaggataat aagcaatttg tgtagatgtt
    10921 aactgatttg aaaaagttaa caccttaaat ttggaggtga gaacttggca caaaggaatt
    10981 tagattatcg gctattaaaa gaccggcgta atgaatatgg cgtttcacaa aataaattag
    11041 ctacgacttg tggacttagt cgcccgtact taaatcagat agaaaatggt ggcgtaaccg
    11101 catcgacaaa aaccatgagg aaaatttttg accaactgga aagtttcaat cccgagttac
    11161 ctttaacgtt actgtttgac tatgtaagga ttcgctttcc cacaacggat gcgaggaaaa
    11221 tcattcaaga gattctccat ttgaaatttg attatatgct ccacgaagat tacgcctttt
    11281 actcctatca agaacaatat gttatgggag atattgtggt gatgttgtca catgaagaag
    11341 ataaaggcgt ccttttagaa ttaaagggtc gtggttgtcg gcagtttgaa acttttttac
    11401 tcgcccaaaa gcgtagctgg tatgactttt tcgaagattg tctaaaagcc ggtggcgtga
    11461 tgaaacgctt agacctggca atcaatgatc gagttggcct gttagatatt cctgatttaa
    11521 cgaaaaaatg tcagaaggaa gaatgtatct ccttgtttcg taccttcaaa agctatcgtt
    11581 ccggggaact gttgaaacct gatgataaag atggtatggg caatactttg tatattggca
    11641 gtcttaagag tgaggtctat ttctgtttgt acgaaaaaga ctatgagcaa tatatcaagt
    11701 tagggattcc tttagaccag accgaaacga aaaatcgttt tgaaatccga ctgaaaaatg
    11761 atcgagctta tcatgcaatt caagatttgt taaaaggtcg cagtattgaa agtaccacat
    11821 tttccattat taaccggtac ttgcgatttg cggataaggt ggaaggcaaa aggcgaacta
    11881 actggccctt gaatgaacaa tgggggcgct tcattggacg gaatcagaag gaaattcaat
    11941 taacttctga accgaagcct tataccattg aacgaacgtt gaactggctt ggacgacaag
    12001 tcgcccctac gtggaagatg gccaaggagt tggatcggtt gaatcagaca acgtacattc
    12061 aggatatggt aaataatact cgactatcag accggcacaa aaagattttg gagcaacaaa
    12121 gtatggcaat tgaaaatttg attatatgaa aagaggaatc gtgatggaaa ttgtgaaaga
    12181 tattttattg gtgtttggtg gcagtatgct tggtgttact gtgatgtgct tgatgcatgc
    12241 aagtgcagcg gcggaccgag caatggaaaa gaaaggaaag tgatgaaatg aatttcggtc
    12301 aaaattt
//
LOCUS       DAJSBL010000073        11198 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.075, whole genome shotgun sequence.
ACCESSION   DAJSBL010000073 DAJSBL010000000
VERSION     DAJSBL010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11198
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.075"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            160..1419
                     /gene="lysA"
                     /locus_tag="ONT85_002460"
     CDS             160..1419
                     /gene="lysA"
                     /locus_tag="ONT85_002460"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HCR2379408.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            1406..2206
                     /gene="dapF"
                     /locus_tag="ONT85_002461"
     CDS             1406..2206
                     /gene="dapF"
                     /locus_tag="ONT85_002461"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HCR2379409.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDTDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            2209..3090
                     /locus_tag="ONT85_002462"
     CDS             2209..3090
                     /locus_tag="ONT85_002462"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HCR2379410.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            3087..3803
                     /locus_tag="ONT85_002463"
     CDS             3087..3803
                     /locus_tag="ONT85_002463"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HCR2379411.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            3809..4504
                     /gene="dapD"
                     /locus_tag="ONT85_002464"
     CDS             3809..4504
                     /gene="dapD"
                     /locus_tag="ONT85_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HCR2379412.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            4520..5521
                     /locus_tag="ONT85_002465"
     CDS             4520..5521
                     /locus_tag="ONT85_002465"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HCR2379413.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            5518..6879
                     /locus_tag="ONT85_002466"
     CDS             5518..6879
                     /locus_tag="ONT85_002466"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HCR2379414.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            6839..7990
                     /locus_tag="ONT85_002467"
     CDS             6839..7990
                     /locus_tag="ONT85_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HCR2379415.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSGAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            7965..9134
                     /locus_tag="ONT85_002468"
     CDS             7965..9134
                     /locus_tag="ONT85_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HCR2379416.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            9309..10688
                     /locus_tag="ONT85_002469"
     CDS             9309..10688
                     /locus_tag="ONT85_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCR2379417.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            10969..11198
                     /locus_tag="ONT85_002470"
                     /pseudo
     CDS             10969..11198
                     /locus_tag="ONT85_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         3729 a         1953 c         2499 g         3017 t
ORIGIN      
        1 tgtttttata accattttta ttcttatttt taaaaaaata tttcgaaata tactagacaa
       61 ttcagaaaat tttgtttaat attattccaa taaaattcaa atctttatca aatgaaattc
      121 caataaaaac ggaaaaaaag tgaaaagagg aatgaaaaaa tgacagaatt gataattggc
      181 ggagtcacag caagcacatt ggcagaaaag tataagacac ctctgtatgt gtatgatgaa
      241 gaaaagatga gacaaacgat gctgtcattc aagcgaggct ttgtatcgaa acgttttgat
      301 acaaaagtgc tttacgcctc taaagcattc caaacgattg agatgctgca tttagttcgt
      361 gaatatggat tcgggctaga tgttgtcagt ggtggggaaa tctataccgc tttgcaagct
      421 gattttccta gagatactat ctacttccat ggtaacaaca agacgccaga ggaactgatc
      481 tatgcacttg aaaatgattt gttgcatatt gttgccgata atttgatgga agttgaatta
      541 ttagcagaat taagccaacg ctatttaaaa aaaatctgta tcatgcttcg gttgaatgtc
      601 ggagtagaag cacatactca tgaatatatc gtcactgcgc atattgattc aaaattcggc
      661 atgagctatg aaagtaaaga gtgccaacaa gcccttcagt tgataaaaga aagcagctat
      721 ctagagttag aaggatttca tgctcatatc gggtcacaaa tttttgatgt tactgcttgg
      781 ttagctgaaa tcgacaaatt gacgaattac ttaaccgatt ttgaccaacc tctttccttg
      841 aatcttggcg gagggtttgg gattcgttat acacaaaatg atcagccgat cccagtcgaa
      901 gaggcactaa aaaaactgat tgaatatacg gaagaagctt tggaaaaacg aaaactgacc
      961 attcgtcaat tactgatcga accgggtaga agtattgttg gagaagctgg tacaacacta
     1021 tacacgattg gtttcatcaa aaagacacca cataaaaaat attactttgt agatggcggg
     1081 atgacagata atatccgtcc agctctgtat caggcagaat acgattgtga tttagcagca
     1141 aaactgtcgg aagaaaaaac agagaaagtg acgattgcag gaaaaatgtg tgaatcaggc
     1201 gatgtggtaa ttaaagaagc ttatttgccg aaagcagaat cgggtgatct tttagctgtt
     1261 tattccacgg gagcttatgg atactcgatg agcagcaact acaatcgagc gacacgacca
     1321 gcagttgttt ttgtcaataa tggccaatca agactgattg ttcgcagaca agcattttct
     1381 gatctactaa ggggggaagt ttcctatgat ccttcataaa tataatggct gcggaaatga
     1441 ttttatctta ttagattacg cagaagacac agattatagt caattagccg cttctttatg
     1501 cacaaaagaa aaatatgata cagacggatt gatcgccgta aaaatcgatc cgttggaaat
     1561 gatctattac aacaaagatg gcagccgcgc gccgatgtgc ggaaatggca ttcgctgttt
     1621 tgctcgatac gtaaatgatc agaacatgat gaatgaacag gaattcaacg tagagacatt
     1681 agcaggcaca agaaaaatta cggtgttgga tccctcacct ttttattgca gcgtagatat
     1741 gggagtgcca gatgattctc cggaaaaaat caaagctaat caaacttatc caataaaaaa
     1801 ccaggaaata gagatcaatg gtgaaaaagt aacgatcact agtttatttt taggaacgat
     1861 tcatacagtt gtttttgttg aaaatgcgcg tgaggaagtc tggagaaaaa gaggtgaact
     1921 gatctgtcat catcctttgt tcaaagaaaa aaccaatgtc aactttgtag aagtagtcaa
     1981 tgaacaagaa ttgatcgtcc gtacctatga aagaggagta ggttggacac ttgcttgcgg
     2041 tacaggttgc tgtgcctctt atgtggttgc taaggaaact gggaaaattt cagcagatca
     2101 ggtagttgtt cacttggaac aaggagactt gatgatctct ggtgaggatg cgatacaaat
     2161 ggctggccca gcagttcatg aatggacaag aatattggag gaatgaagat gataaaaggt
     2221 tcgatcgtgg cgctgatcac gccgatgaat gaagagggat cagtagatta tgctggtttg
     2281 gaaaaattga tccaattcca tttagatgaa caaacagatg gattattagt tttaggtaca
     2341 acaggagaat cttctacgct cacacaatct gaggaggagc agatccttca actgacagta
     2401 aagaaagtgg ctggccgtgt gccggtgatt gcaggagctg gaacgaataa tacgaaagaa
     2461 acgattgaaa aagcgaagca ttttgcttct ttgggggcag acgcactttt ggtcatcacc
     2521 ccttactaca acaaaacaag tgatgcagga ctagctgctc attttacagc aattgcagaa
     2581 gcctcaccga ttccgcttat tttgtataat gttccttcta gaacaggcat gtcgattcca
     2641 atccatgtat tagtaaattt agcagaacat cctaatatca ttggtttgaa agaagcctca
     2701 ggagatatgg cttatgtgat ggatgccgct cggttgattg gagaagaatt cttcctttac
     2761 agtggaaatg atgatttgat cctaccagta atgagtgtag gaggaagcgg tgtaataagt
     2821 gtatgggcga atatccaacc taaaatagtc catgaattag taaaagatac gcaggacgga
     2881 agatggcagc aagcaaaaga aaagcaatta aatgcacttg aactgatcca tgccttgttc
     2941 agtgagacca atcctattcc tgtcaaagca gccatgtcat tattagactt accttccgga
     3001 cctctacgcc tgcctttagt ttctctttca aaagagaaaa agaaacaatt agctcagctt
     3061 ttgttaaaag aaaggacggt tgaaaaatga aaatcggact gattggcagt ggacagatgg
     3121 gaaatagagt cgaagaagtt gcacaagaaa gaggagatac ggtccttctt ttacggacgg
     3181 cacctaaaga agcaactacg cttgctttta cggaactgcc agaactgctt attgattttt
     3241 ctcatccaga taatttagag aaaatccttt cgtatagctt atcatatcaa cttccgttag
     3301 tcattggcac gacaggttat acacaatcac agttccaaga aataaaagaa catgccaaac
     3361 atgtacctgt gatgtatagt gctaattttt cgttcggcat catggtgatg aatcaactaa
     3421 tcaagcaagc agctgcaatg ttgcaaggct ggcagatcga actgattgaa aaacatcata
     3481 gtcgtaaaaa agatgcacca tctggtactg caaatatgct gcttcaaaca atccaagaag
     3541 tgcaaaagct gcagcccgta tacaactggc aagggaaaaa aagagaagaa aaccaaattg
     3601 gtgtacacag tattcgtgca ggttctttac ctggtgaaca tgatgtattg tttgctgcta
     3661 ctgatgaaat atttacgatc aaacatgaat ctttttctaa tcgaatcttt gctgcaggcg
     3721 cagtagaagc agcggactgg ttgaaagatc agtcaccagg ttattacaaa ttagaagatt
     3781 tattaatgga caaaggggtg taaaaaacgt ggaagctcaa gaaatcatcc gctacattca
     3841 aacggcatct aaaaagacgc ctgtgaaagt ctatctgaat gtaaccgagc caatcgaatt
     3901 taaagaaagt aaagtttttg gtgaaggaag cagcttcgtt gtttttggtg actatgagac
     3961 gattgctccg gttttagaag ctgaatcaga aaaaatcatt gaaatcgaaa tcgaaactgc
     4021 tgcaagatac agcgcagttc ctttgctaga tattaaaaaa attaatgctc gtatcgaacc
     4081 aggtgctatt atcagagatc aagtgtcaat cggcaacaat gccgtgatca tgatgggagc
     4141 gattatcaat attggtgcag taatcggtga aaacacgatg attgatatgg gtgctgtctt
     4201 aggtggacga gcgacagtgg gcaaaaactg ccatattggt gcaggagctg tcttagcagg
     4261 tgtgatcgaa ccagcatcgg ccaaaccggt tatcgtagaa gacggcgtac tggtaggtgc
     4321 gaatgcagtg attgttgaag gtgtccatat cggaaaagat gcagtcgtgg cagcaggagc
     4381 agtggtttta gaagatgtgg cagcagaaac tgttgtcggt ggaatacctg cacgtgtatt
     4441 aaaaataagt gatgatcaga caaaagaaaa tacagcattg attgctgcgt tacgcgaatt
     4501 atagaggaga tggagaaaaa tgcgcgaaat agatacctgt attgtggggg caaccggatt
     4561 agtcggccaa acgatgctca aagtgttaga agaatatgat tttccagtag gacgattgaa
     4621 attattagct tcaaaacgtt cagccggtaa agtttgtgta ttcaagaaaa agaaacacac
     4681 tgtacaggaa ttgacagaaa cttcatttga aggatatgat ttagcgctat tttctgcagg
     4741 tgcttctgta tccaaagagt ttgcgccaat cgcacgaagt cagggactat ttgtcataga
     4801 taattcctca gcatggcggg aagattcgga tattccatta atcgttccag aggtcaatct
     4861 ggaagactat acattgaatc ggctgattgc aaatccaaat tgttcaacca ttcaagcggt
     4921 acttccgctt aaagctttac aagaagcctt tggtatccag cgagtcaatt attcgaccta
     4981 tcaagcagtt tctggttccg ggcaaaaagg cattgaagat cttcatcgta cgcaaaatga
     5041 cgaggaacca gttttgtatc cttataatat tagccaaacg gtgattccgg aaatcgatca
     5101 gcctttggaa aatgggtata caaaagaaga acagaaaatg ataaatgaga cccgtaagat
     5161 tctgcatcag cctgctttgc cagtatcagc aacctgcgta cgtgtgccaa tcgaaaatgg
     5221 acatggagtc tctgtggcag ttcaactgca aaaagagttt acgacggaag aaattagaga
     5281 gtgtctcagc cagtttccag ggattcaatt agaagatgat ctagcagcac ataaatatcc
     5341 gacgagcatt cttgctagag gaacagatat ggtttatgtt gggaggatca gaaaagacac
     5401 ttctttagag aacggattgt tgttctatac gactgccgat aacatacgca aaggagccgc
     5461 tgcgaatgcc gtacagattg ctgcagcttt aatgcagaaa atagcggagg tggcaagatg
     5521 agtaatacgc tgaaagtagc gaaattcggc ggaacatcga tgtctcatga agagcaattc
     5581 aaaaaagtcc gtacaattgt tctttcagat cccaatcgcc aagtgatcgt tgtttctgct
     5641 ttaggcaaac gtacgtcaga tgatgacaaa gtaacagact tactttattt gatttatgct
     5701 taccttcagc atcatgttga ttggacaccc ctttggaaaa ggatttgtga tcgtttcatt
     5761 caagtaagag atgttcttgc cctgcagtta cctattgaag atgaactagc acgcatacag
     5821 aaacaactgg aagacgaaaa gataactgaa gattatttag taagtagagg tgaatatctg
     5881 actgctcaac tgatggctga gtatcttggt taccagttta ttgacgcaaa agacctgatt
     5941 ttctttgact atgatgggaa aatcgatcag gaaaaaacag cacaggaaat cacaaaagca
     6001 tttaaaagat atccgaaaat ggttgttcca gggttttacg gggcgaaccc tgcaggaaaa
     6061 gaaaaattat tgggccgtgg gggaagcgac attaccggcg cgatactggc gaagatgttg
     6121 caagcggaaa gttatgagaa ttggacggat gtttcaggaa tcatgatggc tgatccgcga
     6181 atcatcgatc atcctaaaat catcgaggaa atctctttta gagaactgag agagatggct
     6241 tatatggggg ctaacgttct tcatgaagca gctgttttcc cagttcaaga ggctgatatc
     6301 cccatccaaa taaaaaatac caatgatcct ttcgctcaag ggacaaaaat atcgaatcac
     6361 gaaatagaaa aggaaaatgg actgacagga atagctggac gcaaggattt tctttccatc
     6421 actttattca aacgtcatat gtccgatgaa atcgggttta tctggaaagc aatgagcatt
     6481 tttgctaaac atgagatatc aatcgaacat attccttcag ggatcgacaa tatcggagtg
     6541 atcgtctcag cagaagcgat tgccgataag ttatttctca tcacgaaaga attaaaagaa
     6601 acacttggcg tggaagaaat cgtcgtgatc gaagacttag ctttgatctc tgtcgttggg
     6661 ggtccgcata aagaattgat tggattatct gggaaagtgt tatcgatatt gaacaaatta
     6721 gaaattcgca catcgatctt atcacaagga gcacaggagt tgaatctgat catcggtgta
     6781 ccgaataatc aatatgaaac agtagtgaag ggtatctatg aagggatggt gaatacggat
     6841 gctgaaagaa tatcgaatgg cactccatca aattcctgaa atcgggtttc atgaattcaa
     6901 gacaaaaaaa tatttatatg aacaagtaaa aaacagcggg gccgttatcc atgaaatcgg
     6961 cgaaacagga ctactccttt attttgataa tcagcaagaa gaaacgattg cttttcgaac
     7021 agatatcgat gcactgccag tcaaagaaga aacgaatctc tcttttgctt ctaagcatga
     7081 aggttatatg catgcatgtg gtcatgacgg acatatggcg atgcttttag ggttgactga
     7141 ctttataact aaacacattg aggatcttat ttataatgta gtactgatct atcagccatc
     7201 agaagaaatt tccggtggcg cacaaagcgt tattcagtca ggattgttgg ataaatatga
     7261 agtacaagca atatttggct ttcatctatg gcctggacta gctaagggag aagttttctc
     7321 tcgaccagga cctttgatgg cacaaagcag tgaaacagac attatcatta aaggccagtc
     7381 tgcacatatt gcttctagtg aaaaaggaat cgatagtctg gaggcagctg tacgattttt
     7441 gaagaatgtt tatgattttg accggcaatt accggcagag tggaatcatc taatgaaatt
     7501 tggccaaatc caaggtggaa cgatccgcaa tgttttagct ggagaagtta ttgtatccgg
     7561 aagtatccgt tcttacagtt cagatatgca aaattatttg aagaggggat tagaccattt
     7621 agcagaacag tttcactcta cagctactat ttcttttcgc tataatgacg gctatccagc
     7681 tgtaatcaac catagaggct tatatgcagc actgttagat tcaaacaaac tacatgaatt
     7741 agatgctcca gttcttcaag ccgaagattt tggcatatat acgcaacaat atccatgtgt
     7801 atttttcttt ttaggtgtcg gtgatacccc tgctttacat gatccgacgt tcgattttga
     7861 tatggatgtt ttagaaaatg gtgtagaatg gtacaaaacg attcttttta cagaaaatct
     7921 tcttgtaaaa atcgatagca gttcaaaaga ggagcgagta aataatgatg aatttatcca
     7981 atcgatttag tcctaggctt tctagaatag aagtatcaaa aatacgacaa ttcgatcagc
     8041 aaatatcgtc gataccagat gtgatcaaac tcaccctggg agaaccggat tttccaactc
     8101 ctgaacatgt caagcaagca ggaatagcag cgattgaaga agacttttct cactataccg
     8161 gaatgagagg attagaggaa ctgcgggaag cggcttgtat atttcagcaa cagcgttatg
     8221 gattaactta tgatccgcaa acggaagttt tgacgacagt tggtgcaacg gaagcgattg
     8281 cctctgcgtt gttatctgtt ttggaagaag gagacaaggt tcttattcca gcgcctgctt
     8341 attcaggata tcagccatta gtggaactag ctggtgctga attgatcccg atagatactt
     8401 ctgacacagg gtttgtctgt cagccggaac aatttgaacg agcatttgaa caatatggat
     8461 cagctgtcaa agcagtcatt ttaaactatc ctaacaatcc tactgggacg acattgtctg
     8521 caaaacaatt gaaagcaatc gctgaggttt taaaaaaata tccggtattt gttataagtg
     8581 atgaagtata tgctgaattg acttatagtg gaacgcatat gtccatagct acttatcttc
     8641 cagaacaaac aatcgtgata agcgggttat ccaagtcaca tgcgatgact ggttggcgag
     8701 tgggctttat ttttgctcaa aaagccctta ttgacgaact aattaaagtc catcaatatc
     8761 tggtgactgc cgctacaacg atgtcgcaaa aagctgcggc tgaagcgcta ataaatgggg
     8821 tgaaagatag cgagaatatg aaagagcaat atcaaacacg aagagactat ctggtccagc
     8881 aattggcgcc tttaggattt gaagtgaccc agccaaacgg tgcattttac ttgttttgca
     8941 aactcccagc tgccattcaa actgactctt ggcagttttg tgtagaatta gcggaacagg
     9001 caaaagttgc gtgtatccca ggaattgcat ttggcccaga aggagaaggt tatatccgga
     9061 tttcttatgc tagcagcatg gaaaaattac atgaggcctg caaacggatc aaagactttt
     9121 taacagaaca gtgattcata gattttatca aaaaaacaat cgttaatttt gcggtatatt
     9181 ctgcaaggag acgattgttt tttaaattta gttcaaatga acgcgtttct tttgattcat
     9241 aactaatgta atgttaagtt taaattgttt agaatgattt taaaagaata ttcaaggagg
     9301 tttcatcaat ggaaaaaatc aaagttgtca ttgtaggtgc atctcatgga gggcatcaat
     9361 cgatcttgga actattgaca cgatacggtg aaaatgtaga tatcacgcta tttgaagcag
     9421 gagactatat ttccttcatg tcatgtggta tggagctgta tttagaagac aaggtaacag
     9481 atgtaaatga tgtacgtaat ttcagaccag aaaactttcc gcaatccaat gttcatattt
     9541 taaataatca cgaagtaaaa acgatcgatg ccgataaaaa aacagtaacg gttactagcg
     9601 catcagataa cgaaacaaaa gaatatcctt atgacaaatt gattttgagt tctggtgtca
     9661 aaccaaacaa tctgccagta ccaggagcag atctagaaaa tgtctacttg atgcgtggat
     9721 acaattgggc aaccaaaatc aaagaaaaat taaatgatcc ggctattaaa aaaatcgctg
     9781 tcgtaggtgc gggttacatt gggatcgaag cagcagaagc aagccgaaaa gctgggaaag
     9841 aagtcgtatt gcttgatgtg atcgatagac ctttaggcac ttatttagat gctgagatga
     9901 cagatatttt agaacaacac ctaaatgaaa acggtattga agttttgaca aatgcaaaaa
     9961 tcgaagcttt tactggtaac ggaaaagttg aggcaatcaa aacaagtgaa aaagaaatcc
    10021 cagcagatct agtcattcaa gctgccggtg tgaaggcaaa tacagaatgg ttaaaaggga
    10081 tcgtcgactt agatgaacgt ggctggatcc aaacaaatga atacttgcaa acgaatcttc
    10141 cagatgtgta tgcagtagga gatgctacac ttgcttattc tatcccagca cgcaaaaaaa
    10201 tgccgatcgc tttagcgact gttgcacgta gagaagcacg ttatgtagta aaacatcttt
    10261 ttgaagaagt accaaacaaa ccgttcggcg gagtcgtagg ttcctctgca ttaagtgtct
    10321 ttgactatca ttttgcagca agcggattga atagctttac agcagcaaaa gcaggagtag
    10381 atgtacgagt gtcttattat gaagatacgt tacgaccaaa atatgtacca gaagaacaag
    10441 gaaatccaaa agtgtccatc caattattct cggatccaat gacccatcaa ttgctaggag
    10501 gagcagtatt atctacctat gatgttacag gtcaaggaaa tgtattggca ctagcgatcc
    10561 aaaacaaact gactttagaa gaactagcag aagctgactt cttcttccag ccaggctttg
    10621 accgacaatg gagcatgttg aatctagccg ctcaacaagc gttaggagaa gaaccatttg
    10681 tagaataaga aaacgaaata tttgaaaaga ggttgtgaaa aagctgcctt gcatcaagta
    10741 ataagaacag ccaagtaaaa acagcttctt atgtttttaa cttggctgga cttattaccg
    10801 cagatgcaag cttttgaaca ccgtttattt tcaaaaaagg gactgtgaca cgatttgtgt
    10861 accgaccccc aaaagttaga ccaaaaaatc taacttttgg aggtcggtat tttttatggt
    10921 taaatatact tttgaactta aattaaagat cgttcatgat tatttggatg gcaaaggcga
    10981 ttggaccgta aaaatccaga tcaagagatt gaagcggaaa tgcttaaaat tcgggagaag
    11041 cacaaggatt atggctgttt acggatgacc aatgaactaa gaaaccgcgg tttctctatc
    11101 aataaaaaga aagttcagcg cttgatcaag aagctaggaa taaaagtaac ggcatacacg
    11161 cgtaaatcac gtcgctacaa ttcttatcga ggaaaagt
//
LOCUS       DAJSBL010000074        11031 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.076, whole genome shotgun sequence.
ACCESSION   DAJSBL010000074 DAJSBL010000000
VERSION     DAJSBL010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11031
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.076"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            131..505
                     /locus_tag="ONT85_002471"
     CDS             131..505
                     /locus_tag="ONT85_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HCR2379418.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(619..1248)
                     /locus_tag="ONT85_002472"
     CDS             complement(619..1248)
                     /locus_tag="ONT85_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HCR2379419.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1382..1454)
                     /locus_tag="ONT85_002473"
     tRNA            complement(1382..1454)
                     /locus_tag="ONT85_002473"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1419..1421),aa:Thr,seq:ggt)
     gene            1668..1741
                     /locus_tag="ONT85_002474"
     tRNA            1668..1741
                     /locus_tag="ONT85_002474"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1702..1704,aa:Asn,seq:gtt)
     gene            complement(1879..2043)
                     /locus_tag="ONT85_002475"
     CDS             complement(1879..2043)
                     /locus_tag="ONT85_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2379420.1"
                     /translation="MKNLIAGGFLFVGGGILMNTDNFGNVIPFLGAACALIGVGMLIY
                     FVFSDDGKYG"
     gene            2338..3666
                     /locus_tag="ONT85_002476"
     CDS             2338..3666
                     /locus_tag="ONT85_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HCR2379421.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            3868..4701
                     /locus_tag="ONT85_002477"
     CDS             3868..4701
                     /locus_tag="ONT85_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HCR2379422.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            4717..5454
                     /locus_tag="ONT85_002478"
     CDS             4717..5454
                     /locus_tag="ONT85_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HCR2379423.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(5609..6535)
                     /locus_tag="ONT85_002479"
     CDS             complement(5609..6535)
                     /locus_tag="ONT85_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HCR2379424.1"
                     /translation="MKENMLTGGVSPDVANPLLDAEITIPKHVELLVEDIFNLPSPHI
                     MPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIGNKLPIVLTGAMRSS
                     NELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTKTHTTNVATFRTPTL
                     GPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGDLLEAIAHTKVDGLV
                     IEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDYEGGGKELEKMGIIF
                     CNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            6810..7643
                     /locus_tag="ONT85_002480"
     CDS             6810..7643
                     /locus_tag="ONT85_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HCR2379425.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            7677..8516
                     /locus_tag="ONT85_002481"
     CDS             7677..8516
                     /locus_tag="ONT85_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCR2379426.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            8542..9102
                     /locus_tag="ONT85_002482"
     CDS             8542..9102
                     /locus_tag="ONT85_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HCR2379427.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            9301..11022
                     /locus_tag="ONT85_002483"
     CDS             9301..11022
                     /locus_tag="ONT85_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HCR2379428.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
BASE COUNT         3762 a         1816 c         2215 g         3238 t
ORIGIN      
        1 ctacactctg agatgtaaga aatatcataa gatatttctt acatcttttt gttttgagca
       61 ggactttaga taattttatt aatatatgct agcatgcttt tgtaagaaaa agaaagaggg
      121 cgtattagtt atgtatctta aaattacgga acaagcacaa gaaagattac aaaaatatat
      181 tgatgaaggc gcaacagtta tattggattt agatgatggc gttggggaat attctaaaat
      241 gggtgtttgc tctttagata ctagttttcg tttacttctt cttgataaag aacagagaaa
      301 aaatgattat aaactccaat tagatagtga tattggggat gtttatataa aagattattc
      361 aaaaatgtac atggatgaac atatgaccct ttctttagat ccacgtctag gtgtatttac
      421 tcttgaaagc ccaagtggtt ctttagacag tcatgttcaa ttagtagatc tacgaccaga
      481 aaaattctct ggaataaaac aataactttc tctctattgt ccataaaact agtctcaatc
      541 aagctgtatt gaaaaaacaa gacaacattc ttaccatggt aaataaaaag acgacacatt
      601 ttgtcgtctt tttttaaatt aactattttc gataaaagat cgcatagctt gtgccattat
      661 ttctggcttt tctctattca attcatgttt tccgttttta ataaagagta gccggccatt
      721 cgttgaaagt ttatataaac gttttgctgc tggtttattc gcatgatctt tggtaccgca
      781 taaaatcaat ttaggaagtc gtgatgtacg aagctgtttt ttgcagtcta atgtttcaag
      841 tgaacgaagt agggtaaggg tttgtttttt agatagacct aatttttcaa atatcttaga
      901 aggtacgcag taaaaataaa tattctggaa agtaagcaat aagcgattag gagactcaaa
      961 ttgtggagcg actaaaacaa ccttttttaa atatggagaa ggagttgcaa gatagtttaa
     1021 agcaataaca gcgcctaaag ataatccaca taaaataaaa ggttccttta tctgattcaa
     1081 ttgtacttcg atgtatttgg ttaagtcgtc taaagtagca gtgtctgtgc tgtttaaatg
     1141 attgaacaat gaaagacttt tgcaagaata actattgggt agataagaga ttacatcttt
     1201 ccatgattgt tcgtcttggc ctagaccatg caaaaaaaca attttcatat tgctctcctt
     1261 tttatagtta tcatatagac tttctttgta ctattaaaat ataaacattc atttcttacg
     1321 tcataaaata aaaaaacagc gcagacacat tgttaaacca acatgttgca ctgtttaagg
     1381 aagccacttg ccgggcttga accggcgacc tcttccttac catggaagcg ctctaccagc
     1441 tgagctaaag cggcattatc attaatacaa ataataggat aacgaatatt aatgtatttg
     1501 tcaacagaat tttttcgaaa aaatgtgttt ctctaatagg aaaacagatt ttagaaaaaa
     1561 taaaaataac aaaaaatgcg aacggtttct ttgataatat aataaaaaaa ttcaaaaaaa
     1621 gtattgcgtt atttagaaga aattgatatt atattcatgt acttgattcc ggcatagctc
     1681 agttggtagt agcgcatgac tgttaatcat gatgtcgtag gttcgagtcc tactgccgga
     1741 gtaaataaag cttatgcaaa gaaaaagaaa tccaatcact tgggtttctt tttcttttat
     1801 tattatgatg aatcaagctt tctgtttttg tcacgtatgt tgtaatcttt acgaaagagt
     1861 agagttggct actcataatc aaccatactt cccatcatcg ctaaatacga aataaatcag
     1921 cattcctaca ccaatcaaag cacaagctgc accaagaaaa gggatgacat tcccaaagtt
     1981 atctgtattc ataagtatgc caccacccac aaacaaaaag ccgccagcaa tcaggttttt
     2041 cattttattc atctcctttc ttacgttata gtatttcctt ctttcaatga gtattttgaa
     2101 agctatctta ttgtatgatg ttcttctagg cctcttcttt attatacagg ccattttcgt
     2161 tatctcatag aaatattcct ttttatctga aaactattgt ataccgtatg acatttttct
     2221 tcatgaagtt gtaagtatat tctcattcaa atgaaaattt catcatagaa gagattgtat
     2281 ttctaaattc ttaagagtaa aatacgaagt ataacttgga tttggaagga tgattggatg
     2341 aaagctatta cgcctgatat gacaacaaaa tttaacaaac agtttcaagc aaatatcaaa
     2401 cagcaggcat tgcagcgggc agttgtgaag aacggaatta ctgcttctgc tgaaaatgtt
     2461 tcagcacatg tggagaatac accggtattt tctatagatt tggctacggg gaaagtggct
     2521 aatcaaatgc aatcggggcg ttgctggatg tttgctgcac tgaacacatt ccgtcataag
     2581 atgctgaatc aattccattt aaaagagttt gaattatcac aaaattacac ttttttctgg
     2641 gataaatatg aaaaggcaaa ttatttctat gaaaacatca ttgcaacagg aaaagaggca
     2701 atcgatagcc ggaaagtagc tttcttactc gctactcctc aacaagatgg cggccaatgg
     2761 gatatgatcg tttcattatt ccaaaaatat ggcgttgtgc ctaaatcggt gatgccggaa
     2821 agtgcgaata gttcaaattc tcgagatttg aataactact tgaacaaatt actgcgtaaa
     2881 gatgcagtag tgttacggaa aatggttgct caaggagaaa cattagacgc tattgaagaa
     2941 aagaaagaag aaatgctaga aagcatttat aacttcttag ctatctcatt aggaacacct
     3001 ccaaaagaat tcgattttga gtaccgcgat gaagaaaaga actatcatct tgaccgagga
     3061 ctgacaccac aaatttttta tgataaatac atcggtgtaa aattagatga ttatgttagc
     3121 gtgatcaatg caccaacaaa agacaaacca ttcaatcgtt cttatacagt agagatgcta
     3181 ggaaatgtcg ttggcgggaa agaagtcaaa tatctgaatg ttgatatgga aactttcaaa
     3241 aaattagcga ttgctcagct tgaagaagga gaatctgttt ggtttggttg tgatgtaggt
     3301 caatcttcta ctagaactag tggcatcatg gccttagatg cttattcaat ggatgatcta
     3361 tttgatacgg attttacaat gactaaagct gaacgtttag attttggaga aagtctgatg
     3421 acccatgcga tggtattgac cggtgttgac ttagtagacg ggcaatcaac aaaatggaaa
     3481 gtcgaaaata gctggggcga aaaagttgga aagaatggct tctttgttat gagtgatgcc
     3541 tggatggacg aatatacgta ccaaatcgtt gtaagaaaag atttattgac gaaagagcaa
     3601 ttaaatgcgt tcaatgaaga accaattgtt ctatctccgt gggatccaat gggtgcttta
     3661 gcatagacga gtatttcaat gaagagtatg tagtaaagat aaaaagagct ggcttaagcc
     3721 agctcttttt atctttaaag tccattccct gttttttatg gtccctgtgc tataattcta
     3781 aataaaggat aaggattgga aataatcctt ttatcattaa aaatatgatg acgaagtaga
     3841 ataaaattgg gagtaggggg gaaagtcatg gatatccaaa ctgttttatc tgatctgaaa
     3901 ttaaatggta aagtgattcc agttgttggt ggcgatgtta atcagaccta tcgtataaaa
     3961 accgaacatc gtgcttattt tttaaaaatc catccgaatg taaaaaaagg attttttgaa
     4021 gcagaagtag acggattaaa agaactgtca gcattcgtac gtgtaccgga tacgtatatg
     4081 ctaggtgaaa caagtgaagg cgcttatttg ttaatggaat ggatcgagcc gggaaaaggg
     4141 gatcaacgag atcttgctgc agctttagct aatctccatc agcaaacagc gccacaattt
     4201 ggttttcgta aagataacta tttgggtaca cttgttcaga aaaattcttt tgaagaagat
     4261 tggtggactt ttttctttaa agatcggctt gaatcccaga tttctttggc agaagaaacg
     4321 aatcgatgga atgtacagcg tcaagaaaag tatctgcgat ttaaagaacg tgtattgaaa
     4381 tcggttgaac caaaaaagat cactccacgc ctcttgcacg gagacctgtg gagtggtaat
     4441 gtgttctttg accagcaagg gcatccagtt tttgtcgacc cagctgtttc ttatgggaat
     4501 cgggaacaag atattgcaat gagccagtta ttcggtggtt tccgaccgga atttttagat
     4561 gcttatcaaa ctatatttcc attagaaaag ggatggaaag accgactgcc gatttaccag
     4621 ttatattatc ttttggcaca tttgaatatg tttggagaaa gttacggttc ccaagtagat
     4681 caattactag aaaattttta agaggagggc gtgtttttgg caaaaaaata taccaaaaag
     4741 catgaagttt catattatga gtgtgacatc aaccaaacga tgacatttcc ttcgatgtta
     4801 ggagtagtta tcaaaacgtc tgaagatcaa agcgaccagt tgggaagagg cagtgaattc
     4861 gtcaatagct ttggacttac ttgggtcatc acgaattatt ccatggagat taatcgtctc
     4921 cctagagtcg gtgaagtaat ctcagtaacg actcaagcca tggagtacaa taagtttttc
     4981 tgttatcgga acttctggat ccatgatgaa gatgggaaag aattagtcaa aatagaatct
     5041 gttttcgtat tgatggattt tgtgaatcga aaaatgagca gtgttaatga agagatcatt
     5101 gctccttttg agagtgagaa gatcaagaaa atcaagcgtc aggaaaaaat tgaaaagatt
     5161 gattcgggct tcatgttgcc atatcgtgtt cgtttttatg atatcgatag taatcagcat
     5221 gttaataatg cgatgtattt taattggatc attgacgtat taggatatga ctttttgact
     5281 acgcatgttc cagaaaaagt aattattcga tttgataaag aagtagagta cgggaatgag
     5341 atcgaaagcc attttgaaca aatccctatc gaaaatggtg tcaaaacaaa acatgaaatc
     5401 cgaatgcaag aacagctgta ttgtgaagca aatattatat ggaaaaataa ataaaaaaga
     5461 ggttgtgaca gaagggggca acttcaagaa ataagaagaa atttacgaaa attgtttctc
     5521 aaatttttgt aaattttcga cttagttttc gaagaagcta tttttgtcac agcctctctt
     5581 tttaagttta gctattagat aatgatgttt agttttggat aaacgaaatc aattcttcac
     5641 ccgacaaacc atagtttaca gcaatcaata gttttaaacg tgctttttga ctattaatac
     5701 tgttacagaa aataatcccc attttttcta gttcttttcc cccgccttca taatcataaa
     5761 caggctctgc gattccattg aaacaacgag aaacaagtac tacagggatt ttttggacaa
     5821 gaagcttttc caaagcagct aaggcttgtg gagggagatt ccccgcacct aaagcttcga
     5881 ttaccaaacc gtctactttc gtatgggcga tggcttccaa taagtcccct tgcatcccag
     5941 catatgcttt aacgatcggg atagtcccat ctacagcgga aatgtccagt cgctttgttt
     6001 ctaaaagttc ctgcaagaat aaaattcgat tcttagttac taagccgatg ggacccaatg
     6061 taggtgttcg gaaagtagca acatttgtgg tatgggtctt tgtcacgtag cgtgcagaat
     6121 ggatctcatc gttcatgacc accaaaaccc ccttatctag cgcttcttca caacttgcga
     6181 cacgaatcgc actttcaaaa ttataaaggc cgtcacttcc aagttcatta cttgaacgca
     6241 ttgccccagt caaaacaatt ggtaatttgt tgccaatcgt tgtatcaaga aaaaaagcag
     6301 tttcttctaa tgtatctgta ccgtgtgtga tcaccactcc ggatatacct gatgcataag
     6361 cggacaagat acgttctttt aataccaaca tgtgagaagg catgatgtgt ggtgagggaa
     6421 gattgaagat atcttcaact aataattcta catgcttggg gattgtgatt tctgcatcta
     6481 ataatgggtt tgccacatca ggactaacac cgcccgtgag catgttttct ttcatggcga
     6541 tcgttccgcc ggtgtgtagt acaagtattt tttttcattt tacagacttc cttacgaata
     6601 attttgcata gccgttaaat cttattgtat actatataaa agagttttgc tatcagtgaa
     6661 gaataacgaa tagcctaact tatcataaaa aagctgacct tattaaaatg gaaaagactc
     6721 aaccttgacc tcctaataca atggagtatc tggttgagtt tcttttacaa gaaagcagcc
     6781 aaggaaatca atcacgaagg tggccggaaa tgatcagaat cggattaaca tcgtttaaag
     6841 aacatgacaa attaactggt aaaaagaata acactcttta tgaatatgca gggtttctac
     6901 ccttagtgga attagatact gcctattatg gaattccccg aaagtcctca gttgagaatt
     6961 ggataagtga agtacctgaa tcatttcgct ttgtagtcaa agtctatcaa ggaatcagcg
     7021 gtcaaggaga ctggaagcct tattatcaga ccgaagaaga gatgattgcg cattttttag
     7081 atgttatgtc gccaatggta gatgctggaa agatgttttg ctttttagtc caattcccct
     7141 catcattcaa atgtacgaaa gaaaatgtcc agtacttgcg caaacttaga aagtggtttg
     7201 agggttatcc tgtttctata gaattgcggg attattcttg gtacagcgat gaatttcttg
     7261 aacgtacaag aaactttatg tctagagccg ctttttcttt agccatcatc gatgagccac
     7321 agttgctgaa tacaacgatt ccttttgatc catttgtcac caatccgaac tttgctcttt
     7381 ttcgattcca tggtaggaat gcgcaaggat ggcaagcaaa tgataaagag tggcgtaaaa
     7441 aaagaacgtt atatcgttat tcagaaaaag aactgcaaga actagcacat gaagtcaaag
     7501 tagttgattc taagacaaaa gaggtaggtg tgatttttaa taataattct ggtggtgatg
     7561 cggctgaaaa tgcattagca ctgcaaaaaa tattaaatat caagccagat ggactcaatc
     7621 caacccaaat ggatctgttt taagggctta ttttattcaa gaaaggggcg aagtctatga
     7681 tcaaagctat tttttttgat attgacggaa cattggtcac caatcgttca aaagcgttgg
     7741 aatcaacaaa acaagcaatt gaatatgctc ggaaaaacgg cattttatgc ggtgtagcaa
     7801 cggggcgcag cccagtaaag atcaaagaaa tcattgatga attggaatta gatatgtatg
     7861 tcgtgtataa tggtcaactg gtttttactg cagatcgcac gattatcgac cacccttttg
     7921 aacaaaaagt attggaacat attgttgaat ttgcagatga gaaccatcga caaatcgttt
     7981 ttggtgcgag aaatcggtta gatggatcaa cgacgatgct attaggacaa tcgattttta
     8041 tcaagcgact agtcagcttt ttaccgcgta aattcccggt acgtctgatg aaaaaaatcc
     8101 tccaggtttt tagccctcac cgacaaaagg atcgttatga aacgttagag atattaaaag
     8161 aaccaattta tcaatgtata ttgctcagtc cggagtcaga acagaaaaag ttagaacatc
     8221 gctttcccga gtgtacgttt cagcgttcaa atacgtatac tgtagatatc atcccaaaag
     8281 gtggttcaaa attactaggc atccaagctt tcgcaaatgc tgtcgaaatt gaaatggaag
     8341 aaattatggc attcggcgat cattacaatg atatcgagat gctgaaaggt gtaggaattg
     8401 gagtggccat ggggaatgct cagatcgaag taaaacatgc tgcagacttt gtgactcaat
     8461 caaatgaaga agatgggata tacgaaggac tgaaacactt cggtctgatt tattagtcaa
     8521 tgaaagaagg aaaaaatcag tatggaagca gagacacctt ttcaaaaagt agaaacattc
     8581 cacaaaactt ttgatccaag aagaccagag atacccacgg cttttacaga aaatgaagct
     8641 catacacgtg ctgcgttcaa aatagaagaa cttgttgaat ttgttgcagc tacttcacct
     8701 aataaagaac aactgcatga agctgttatg ctgctgcacg aagcattaga taaagcagaa
     8761 gaaaaaatac ttggtaaaac acagtgggga agtgatccgc tggttgaaca agtagatgca
     8821 ctgacagata tcctttactt tacttatggt tctttttcat tactaggaat agtgcctgat
     8881 cgaatattcg aaatcgtcca tcaggcaaat atggggaaat tatttccaga tggaaaacct
     8941 cattatgacc ctgtaacaaa taaagtgttg aaacctgaaa actggcagat ggaatatgct
     9001 ccagaaccta aaatcgcggc agaaatccaa cgtcagattc aagaagcgat tgctaagaag
     9061 aaatccgctg atataggcat gtgcacaaat gaaaaagagt gatcagagtt tcactatttt
     9121 tgttatcttt atacgatgga aaaacaaaag attttcgttt aagttagtga atgcatgaaa
     9181 atagtatgct ataatctaga atagatgtct tttgcacgag agacgtatta tttggattta
     9241 tgccaagcag aacgccatct gttgcataaa agatgcatac tgaaaacgga ggaaccataa
     9301 atgggaaata atgttatcat cggaatcgtc attgcaatca ttatagtcgc cattgtcgtc
     9361 tttttggtta gttacttgat gaagaaaaaa aaccaagatc gattggatgc cttagaaaaa
     9421 cgcaaggaag aattatttga cttgccagtg attgaagaag tcgatgatgt taaaaaaatg
     9481 catctggttg ggcaaagcca aaataccttt cgtgaatgga gtcaaaaatg gaatgacata
     9541 tctacttcct cgttcgctga actagaaagt caaattttcg aagtggaaaa cctcaatgaa
     9601 agttttcggt tcttaaaagg aaaaaaagca gttgttgaag cagaagctac aatggaccaa
     9661 atggagaaac aagtgacgga aatccgtgaa ggtctaaagg aattgcggga aacggaagaa
     9721 cgcaactcac tagctgttca gcaggcactt gacgtctatg aagaactgaa agaaaaagtc
     9781 agagaagacg agcatgaata cggaccagct ctttttgaat tgcaaaaaca gatcaaagtg
     9841 atcgaaaaag aatttacgca gtttgtgact ttaaacactt ctggagaccc tgttgaagcc
     9901 cgcgaggttt tggataaagc agaaaaacat acgtatgaag tggaagctct gatgaaaaaa
     9961 attccaccgc tgtatgagga gctccatacc actttccctg aacaacttga agaaatcagt
    10021 gatacttacg agaaattgat ggaagaacaa tatgtattcc cagaagaaga tcttgctgaa
    10081 gatatcgcaa aagtaagtcg aagaattgaa aattcattag ccaatcttga aaaaacagaa
    10141 gttgaaacgg tcgaatttga aaaccgtgaa acagctgatt tgattgattc gttatatgat
    10201 atcttagaac gggaaatgga agctcagaga tatgtaaaaa ctaaccaatc aacgattgca
    10261 gagtatatca aacatacaac aaagaataac cgtcaattat tgattgagct ggatcacaca
    10321 tctcaaagct atacattgaa ccacaatgaa attggccgtg tacgaggttt ccaaaccgaa
    10381 gtggaagaaa tggaacgtca aaatgaacag atgattccac agatccgtca acacgaaatt
    10441 ccttattcag aagtgcgcac gttctataaa acagtcttca aagtcttaga agatatcgaa
    10501 aagcaacaag tagagatcga cgactcatta catgaattga gaaaaggcga aaaagaagcg
    10561 caagaaaaga tcgatacatt cgaattcaaa ttgcgcagtt tgaaacgttt tgtagaaaaa
    10621 caacgtttgc ctggcttgcc gaatgattac ctagaattct tttttgttgc tactgaccgt
    10681 attgaagaac taagtgttgt cttgaataaa attcgagtaa acatggaaga ggtcaatcgt
    10741 ttagttgctc tatgtgaaga agatctagaa ttattagata agaagacaca tgatttagta
    10801 gatgcagcag cattaacgga gcaaatgctc caatatgcaa atcgttatcg tcatacacac
    10861 gaagaagtga gagaagcaat tgataaaagc tactatttat tcaataaaga atatcactat
    10921 caagaagcat tagacgaaat cggtacagca ttagagcgag tagaacctgg tgctttcaaa
    10981 cgcatcgaag atttttattt taatcatcca gacttagtat aaagcaaaaa a
//
LOCUS       DAJSBL010000075        10928 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.077, whole genome shotgun sequence.
ACCESSION   DAJSBL010000075 DAJSBL010000000
VERSION     DAJSBL010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10928
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.077"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..287)
                     /locus_tag="ONT85_002484"
     CDS             complement(<1..287)
                     /locus_tag="ONT85_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR2379429.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAE"
     gene            429..1547
                     /locus_tag="ONT85_002485"
     CDS             429..1547
                     /locus_tag="ONT85_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCR2379430.1"
                     /translation="MFEKLRQSKLMFWSLELLIIAALIMISSEINFIFEPIGTFFSTL
                     FAPVLIAGFLYYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQI
                     SSLASNMPDFIKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSL
                     GSIVGTIASATVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTL
                     ANYISGQAIECLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLV
                     TVFNDPVKALLCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLG
                     IFLGVPFYAICKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            1675..3051
                     /locus_tag="ONT85_002486"
     CDS             1675..3051
                     /locus_tag="ONT85_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HCR2379431.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            3452..5032
                     /locus_tag="ONT85_002487"
     CDS             3452..5032
                     /locus_tag="ONT85_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HCR2379432.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            5230..5453
                     /locus_tag="ONT85_002488"
                     /pseudo
     CDS             5230..5453
                     /locus_tag="ONT85_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
     gene            complement(5658..5978)
                     /locus_tag="ONT85_002489"
     CDS             complement(5658..5978)
                     /locus_tag="ONT85_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HCR2379433.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
     gene            complement(6126..8360)
                     /locus_tag="ONT85_002490"
     CDS             complement(6126..8360)
                     /locus_tag="ONT85_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR2379434.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            8839..9105
                     /locus_tag="ONT85_002491"
     CDS             8839..9105
                     /locus_tag="ONT85_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HCR2379435.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            9105..10832
                     /gene="ptsP"
                     /locus_tag="ONT85_002492"
     CDS             9105..10832
                     /gene="ptsP"
                     /locus_tag="ONT85_002492"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCR2379436.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDMMEEV
                     VELVHEYVK"
BASE COUNT         3479 a         1912 c         2215 g         3322 t
ORIGIN      
        1 ttttctgctt cttcaatcaa tatttttcct aacccttggt gtctgtattc agaaagaatc
       61 gccatccgct gtaatttcat ttttccgttt tctaaaggca aaagacgtac agttccttgc
      121 ggttcttttt tatctgagta caggacgaag tggatacaat gggcttcatc tttgtctatt
      181 tctcgagaca aaggcacacc ctgttcgact acaaaaactt gatgcctgat ccgtacagca
      241 tctaagtaaa tattgctcat tgtatctttt gtttgtacaa ctttcatggt ctagccacct
      301 tcctttcgta tagtatactg gaaaataaaa caaataaaaa gtatggcaag ttttcaaaaa
      361 taaaaaaaga ataaaagaat ttttaacgaa tataatatat actagtaaga aagaaaggag
      421 gaaatctatt gtttgaaaaa cttagacagt ctaaactaat gttttggtcg cttgaattat
      481 tgatcatagc tgcattgatc atgatttcat cagaaataaa ctttattttc gaaccgatcg
      541 ggacattttt ttccacgtta tttgcacctg tactgattgc aggctttctt tactatttat
      601 tgaatcctat tgtcaagctg ttgatgaaag caaaaatcaa aagaatatgg gctgtcgcaa
      661 ttgtgctgct ccttttagtt gcagcaatta tttggatttt acttagtgtc atcccaagtc
      721 tagtacagca aatttcctca ttggcatcta atatgcctga tttcatcaaa caggtagaaa
      781 gctggttaaa agaggtagcc gaattgcctt tgttcaagga agtagatatc aataaatatt
      841 ttgagcaatt agacatttct tacgggacaa tcattcagca attccttagt ggagtatcca
      901 atagtttagg ttcgatcgtt ggaacgatcg cttcggcaac cgtagtaatc attacagcac
      961 cattcatctt gttctatatg ttgaaagacg gagaaaaact agtgccaaac atccaacagt
     1021 ttttcccaga aaaaagaaga aaccaaattg tggagttatt ggggcaactg aataaaacat
     1081 tggctaatta cattagcgga caagcaatcg aatgtttatt cgttggtacc ttcacatttt
     1141 taggatatat ggtaattggt gtggattatg ctttcttatt tggggtcatt gcgggattga
     1201 caaacctaat tccatattta ggaccttatc taggactagc acctgcattt ctagtaacag
     1261 tcttcaatga tccggtaaaa gcattacttt gttgtgtggt tgtgctaatc gtccaacagt
     1321 tggatgggaa tatcatttat cctaatgtaa ttgggaaatc actgaaaatc catccattaa
     1381 cgatcattat cgttcttcta gtagctggaa atattgccgg attgcttggt atcttcttag
     1441 gtgttccttt ttacgcaatc tgcaagacga tcatttccta tataatcaaa atcgtcaaag
     1501 aagacaagca aaatgaaaac aagaagaaaa ttgctacgac ctcgtaggta gttttagtac
     1561 acttgttttt attgcaaaga aaattgcttt gtgataccat tttaacgtgg ctatagtgcc
     1621 acgttttttt ctatcgtaaa ctaaaaatta attttataag gagagaatgc gattatgaat
     1681 gctgaccctg agagtcagtc gctgttagcg cagattttat tattgatcgt tttgacattg
     1741 atcaatgctt ttttagcagc ttccgaaatt gctgttgttt cgatcaataa gaatcgaatc
     1801 gagcaaaaag ctgatgaagg agatgtgaaa tctcaaaagc ttttaaaaat attgcagaat
     1861 ccaaataatt tcctatcgac aatccaagta ggaatcactt tggtcaacat tttgtctggt
     1921 gcttcactag ctaatacttt atcggaacgg ttagctccgg tattaggtgg cggcacagcg
     1981 gcaaaaaata ttgcaaatat cattgtgctg gctattttga cctatgtatc gattgttttt
     2041 ggagaattgt accctaaacg tattgcttta aataaatcag aagaagtcgc aaagttcact
     2101 tcagggctga tacgcttgat tggtgtagta gctaagccct tcgtctggct gctttctgct
     2161 tccacaagtt tattggcgcg tatcacgccg atgacttttg atgatgaaga ttcaaaaatg
     2221 accagagatg aaatgcgtta tatgcttgaa aacgaaggcg tattgaataa tgaagaatta
     2281 gaaatgctcc aaggggtttt ttctcttgat acgaaagttg cccgagaagt catggtccca
     2341 cgaacggatg catttatgat tgatatcaac gacactgtgg aagaaaatgt gaatgaagtt
     2401 ttatccgaaa actattcaag aatccctgtt tataacgaag acaaagataa agtagtcgga
     2461 atccttcata caaagaattt actaaaagct gcccataaat tcggctttga taatttagat
     2521 atcaagaaaa tcatgcagga gcctttattc gttcctgaaa cggtctttat tgatgatctg
     2581 ttatatgaaa tgaaaaagac gcagaatcag atggctattt tattagatga atatggcggc
     2641 gtcgttgggt tagcaacact agaagacctt ttagaagaaa tcgtcggaga aatcgatgat
     2701 gaatcagatg aagttgagaa tctttatgaa aaaatcgatg aacacgaata tatcatccaa
     2761 ggacgtatgc tgattgatga gttcaatgaa gcttttgaca gtgatctgca tatgagtgat
     2821 gtagatacga tggctggtta tttgattaca gcattaggca tgattccgga tgaaggcgaa
     2881 aagttatcgt ttgatgtcga taatattacg ctggtttctg aagaaatgga aggttctcga
     2941 atccttaaaa tccgtgtcat ttttcatgat ccggaagaaa cagaagcgga acctgaagaa
     3001 gaacgaagat acttcagaaa agaattcgaa gatgacgagc caagaagata attggaaact
     3061 tggttatgaa agacgtgttg cttgaataaa caagcaacac gtcttttctt ttagtcagct
     3121 gacaaaaaaa tatttgactc ttatggtttg gtggcttttt cattcgctga atccatgcta
     3181 tactgattca gttgtagtag ttgacaaagg ttgtgaaaaa gctatcctgc attaagtaat
     3241 aagagcagcc aagcaaaaac agcttttcgt atttttggct tgggtgggct tattgctgat
     3301 aggctgtctc tagaacgccg tttactcgga aaaagagact gctacacggc ttttgtcaca
     3361 gccctatatg aaattgtctt tctttaagag atcggacaat ctgtgcctag agaaaatata
     3421 aataattgaa tggaagaaat ggagttaggt tatgaataat ccaaaattaa agcaacaagt
     3481 ggacaatcgc agaacttttg cgatcatttc ccatccggat gctgggaaaa caacgattac
     3541 agaacaatta ctattgtttg gtggggctat ccgtcaagcc gggaccgtaa aaggcaaaaa
     3601 aacaggtaat tttgccaaat cagactggat ggaaatcgag aaacaacgtg ggatttctgt
     3661 tactagttct gttatgcagt ttgattatca agggaaacga gtaaatatct tggatacacc
     3721 aggacatgaa gatttttctg aagatactta tcgtactttg atggccgtgg atagtgcggt
     3781 catggtaatc gatagtgcaa aagggatcga agcacagaca aagaaattat tccaagttgt
     3841 aaaaaaacgt ggcatcccga tatttacttt tatcaacaaa ctagatcgtg atggtcgtga
     3901 accattggaa ttgttagaag aattagaaga gctattagat atcgaatctt atccgatgaa
     3961 ttggccaatc ggtatgggta aagggctaga aggtctttat gatatctacc ataatcgtgt
     4021 agagttttat cgtccggaaa attatcagga tgaacgatta gtagaattgg atgaagatgg
     4081 actgcttccc gaaaatcatc ctctaactga aaattcctta tatgaacaag tcttagaaga
     4141 agtagaacta atcaaagagg ctggagatgc atttaatcaa gaaaaaattg ctcgtggcga
     4201 tcagacacca gtattttttg gttctgcatt gactaacttc ggtgtacaga cttttttaga
     4261 gactttcgtt gaattggcac cagctcctta tggacacaaa acacaagatg atcagatggt
     4321 aagtccttat gaggaagaat tttcaggttt cgtattcaag atccaagcaa atatgaatcc
     4381 agcccatcgt gaccgaatcg cttttgtccg aatctgttct ggaacattcg aacgaggaat
     4441 ggatgtctgg ttggaacgta caaataaaaa actgaaatta agcaatgtca cacaatttat
     4501 ggcagattcg agagaaaacg tcgaaaaagc agtagcaggc gatatcatcg gtgtatatga
     4561 tacaggcaat tatcagattg gggatacatt gttcgaagga aaactaaaag ttgcttatga
     4621 agaactccct tcatttactc cagagctgtt tatgaaagtg actgcaaaaa atgtcatgaa
     4681 acaaaaatcc ttccacaaag gaatctatca attagttcaa gaaggagcga ttcagcttta
     4741 taaaacatat ctaacggaag aatatatcat tggagctgtt ggtcagctac aattcgaagt
     4801 attccaatat cgtatgttaa atgagtataa cgccgaagta atcatgtctc caatgggaca
     4861 caaaatcgct cgctggatcg atccagaaga tttggatgag aagatgagtt caagtcgcaa
     4921 tatcttggct cgtgaccgtt tcgaccaacc gttgttctta ttcgagaacc aatttgcaga
     4981 acgctggttt gcggataaat atcccaatgt aaaattaaaa agtttgatgt aaagaagtct
     5041 tgacacaagc tgccctgcat caagtaataa gaatagccaa acaaaaacag cctctcatat
     5101 tttgtttaga gccgtgacgg attttccgtg acggctcttt ttactatatt aaaatcatag
     5161 aaatgtctaa tcaaaaaaat ataaaaatta ttttgcgtac atatgattaa agaaaggaga
     5221 gtgtgacaca tggaaacaga atggcaaaga cgtgaaagaa aaaaacagaa aaagaagaga
     5281 tgttacgatt atgtcgatta ttagatgtaa aagactggga caaaaacgag caaattgcac
     5341 aaaaagtacg atcaatcatc cataaagagg aaaaagttaa aaataaagac acgcgtagaa
     5401 ctgaaaatga aaagagaaaa gtacaaaaga aaagagaaaa atcaaaaagc tagaaagaaa
     5461 aagaggctga gacagaagcg tttaactcca agaaataagc tggaattcac aaaaatttga
     5521 aaagcaattt tcgtgaattt cagcttattt ccgaaggagt tgcttctgct ttcgccgttt
     5581 atacgtgttt agagcgtgag acaaaagtgt tctttacttt tgtcccacgc tctttaatta
     5641 ttatgttcaa aatttgttta cacggctaat gtagcgccta attcatgttt tggaatcgag
     5701 atttcaacaa ggcctgccaa ttcgattact ttgatttcat cctcgtggta aacggcgtct
     5761 ttgattttat ctaagaaata atttttagca tcttcgattt ctttcggctg gattttacgt
     5821 ttgtcattga tcagcagaat ttcattatgt tcaggggctt cagtgtagac aatagtggta
     5881 tttccggctg tgtagacctt gactagatgg gcatcagtgt tttcaagctg gtttaataca
     5941 agacgggaat ggctgtttgt tacatttacc aatttcatgg cgctcacctc ttttatgtat
     6001 ttttaattgc ttgtttctct tattataatt tcttttttat aaaatgaaca gtattttgac
     6061 ttttctgaaa aaagaatgag acggcatttc tcaaagaaat accgtctcac gctataaaac
     6121 tggatttatt cagccgtttc ttcggctgat tctttagcca aacgttctgg tttagaagta
     6181 acgatgattt ttccatcttt atccagtttt gctttcaact catggataga agggtgatca
     6241 agataaaatt cagcgatacc atcttcgatt tgttcttgga tcgtgcgtcg aagaggacga
     6301 gcacccatag atggatcata gcctaagtca actaattttt ctttgacatt cgtaggaact
     6361 tcgatgtgaa gttgctgtgc tgccagcatt tggttgacat cgtctagcat tagtgtgacg
     6421 attgtcatca agttttcttt tgataatgca ctgaattcga tgatcccgtc aaaacggttc
     6481 agaaattctg gtgtaaagta gttgtttaat tggttcaata cagaacgagt aacaccttcg
     6541 cgtgccgcac caaaaccaac gtttgcttct actttaccag ttccggcatt acttgtcata
     6601 atgatgatcg tatctttaaa gctgaccgtt cttccttgtg catctgtcaa tcgaccatca
     6661 tctaagattt gcaagaacat atgcaaaaca tctggatgag ctttttctac ttcatctaac
     6721 aagacaagac tgtaaggatt tctccgtact ttttctgtta attgaccagc ttcttcgtat
     6781 ccgacataac ctggaggaga accaatcaat ttagagacac tatgtttttc catatattca
     6841 gacatatcga aacgaatcat tgaatcttgt gaaccgaata gttcgtaggc aagttgtttc
     6901 gcaagctctg ttttacctac accagttggt ccaacaaata agaaggagcc gattggacga
     6961 ttctgtttgt ttaatccaac acggtttcgg cgaatcgctt tcgctactcg atctacagcg
     7021 ttatcttgtc cgatgacatg agctttcaag tcatctgcta agtttttcag ttgtgtctgt
     7081 tctttttctt ttaattcacc cactggaata cctgtccgtt gttcaacgat tttttccata
     7141 tctttttcgg taatcacagg tgtttcctct tcagtgagct gacgttcttt cattttttga
     7201 agtttattga tctgatcgcg ataataagct gctttttcga aatcttcttc tcgagaagct
     7261 aaaacttttt gttcttctgc ttcttgcagt tttttctcga ttgttttagg atcaacgatt
     7321 tggatcgtca agtttttctt agaacctgtt tcatctagca aatcgatggc tttatccggc
     7381 aagaagcgat cttggacgta acgattggat aaagttgcgg ctgcttcgat ggcctcatca
     7441 gtatatttta catgatgata gtcttcataa cgtttttgca aacctttcaa aatactgatc
     7501 gtttcttcga cagttggttc atctacccgt acaggttgca tacgacgttc taaagcagcg
     7561 tctttttcga tgatgcggta ctcatttaag gtagtagcac caaccatttg caattctcct
     7621 cgagctaaag ctggttttaa aatattacca gcatccatat ttccatcacc agcagaacca
     7681 gcaccgacga tttcgtgaac ttcatctatg aataggatca cattttctgc ttgacggatc
     7741 tcttcaatca atttttgcat acgttcttca aattgtcctc gtataccagt accttggact
     7801 aaagatacga catctaaacg aatgacttct ttgtccatta atttttgcgg tacatcacca
     7861 tctacgattt tttgtgctaa accttcaaca actgctgttt taccaacacc tggttctcca
     7921 atcaagacag gattgttctt ggttcggcga tttaaaattt caatgactcg tttgatttcg
     7981 tcatcgcgtc cgatcacagg atcgatatca ccttgtcgtg cttgttctgt gatattgatt
     8041 ccgtattctc ccaataaacc ttctgtattg ccttgaggtg gctgaccacc attgaagttg
     8101 ttgtttccgc caaattgtgt tggtggtgtt tgctcataat ttccttgctg ttgcatttgg
     8161 cgagacatgg aacggaaaag atcgtccaaa ctgccaaatc caaatggatc attttgggcc
     8221 atattagtca gacctccatt ttgttgattt ttgatttttt gataacagct ctgacagtaa
     8281 tcgagttgtg tccgctgacc attgacagtg gcatacaagt gaatcgttgc ctcattcttg
     8341 ccgcagtttt gacaaagcat gaatcattca cgtcctttca caaaaatggg tacatccata
     8401 ttttacatct cgtttttcat gaggtaaaag aataacccct agttttttaa gagatttatg
     8461 aggtagaaaa ggtgaaatct ctattgacca ttcctgacct ttgactctta ttatactaaa
     8521 aataaaaagg gaagcaagta attcgtttca tatgaaaaca aagagggtca aaggaaagaa
     8581 tgataaacgt caggagaaga gaaagcgaca agaaaaagga aataaacacg tttgttataa
     8641 aatgtgaaga aaaggatgaa aaagaatccg gaataatttg aatactttta tctgaaattg
     8701 aatgaaaact gttgtatcta acgaaaaaaa atgttaatct ttacagatga aagggtggca
     8761 tttgttagga ttctctgaat tactaaaaaa gaaaaccctc acataaaaac aacactcact
     8821 taaaggagac cgattaatat ggaaaagaaa gaatttcacg tagtagcaga aactgggatc
     8881 cacgctcgtc cagctacact attagtacaa acagcaagca aatttaactc tgatgtaaac
     8941 ttagagtata aaggtaaatc agtaaacttg aaatcaatca tgggcgttat gtctttaggt
     9001 gtaggtcaag gttctgatgt tactattact gcagaaggtg ctgacgaagc tgatgcaatg
     9061 gcagcaatcg ttgaaacaat gaagaaagaa ggcttatctg aataatggtt gaaatgctaa
     9121 aagggattgc cgctagtgac ggtgtagccg ttgcaaaagc ttacctgcta gttcaaccgg
     9181 atttaacatt cagtaaggca acagtagaag atactgcggc tgaagaagct cgtttggatg
     9241 ctgcattagc taaatctact gaagaattgc agcaaattcg tgaaaaagca gcgcaaagct
     9301 taggtgaagc agaagcgcaa gtatttgatg cccatttgat ggttctttca gatccagaaa
     9361 tggtaggtca aatcaagcaa aatattaaag acaacagcgt aaatgctgaa tcagcgctaa
     9421 aagaagtaac ggatatgtat atcggtatgt tcgaagcaat ggaagataat gcttatatgc
     9481 aagagcgtgc tgcagatatt cgtgacgttg ctaaacgtat tttagcgcac ttattaggcg
     9541 ttactttacc aaatccatca atgatcaacg aagaagtggt cgttgttgca catgacttga
     9601 ctccaagtga tacagcacaa ttagatcgca attttgtaaa agcgtttgta acagacattg
     9661 gtggacgtac ttcacattct gctatcatgg cacgttcttt agaaattcca gcaatcgttg
     9721 ggacaaaaga aattactgct aaagtcaaag aaggcgatat tttggctgtc aacgggatcg
     9781 aaggcgatgt aatcatcgat cctacagatg aacaaaaagc tgaatttgaa aaagcaggtg
     9841 cagattacgc agcacaaaaa gcagaatggg aaaaattaaa aaatgcagaa actgtcacag
     9901 ctgacggcaa gcattttgaa ttagctgcaa acatcggaac accaaaagat ctagttggtg
     9961 tccataataa tggtggagaa gcagttggtc tgtaccgtac tgaattcctg tatatggatt
    10021 ctccagactt cccaacagaa gatgaccaat atgaagctta caaagctgta ttagaaggta
    10081 tggaaggaaa acctgtagtt gttcgtacaa tggacatcgg tggagataaa gaactaccat
    10141 atctgcaatt acctcatgaa atgaatccat tcttaggata tcgtgcattg cgtatcagtt
    10201 tatctgaaca aggcgacgaa atgttccgta cacaaatgcg tgcattgctt cgtgcatctg
    10261 ttcatggtaa tttacgtatc atgttcccta tggtagctac actcaaagaa ttccgcgcag
    10321 caaaagcgat ctttgaagaa gaaaaacaaa aattgataag tgaaggcaag gaagtatctg
    10381 atacgatcca agtcggcatc atgatcgaga ttccagcagc agcagtctta gcagacaagt
    10441 ttgcaaaaga agtagatttc ttctctgtag gtacgaacga tttgattcaa tacacaatgg
    10501 cagcagaccg tatgaacgaa cgcgtttctt acttgtacca accatataac ccatcaattt
    10561 tacgtttgat caaaaatgta attgatgcag cacacgctga aggaaaatgg gcaggtatgt
    10621 gtggagaaat ggctggagat caaacagctg ttccattgct agtaggtatg ggtctagatg
    10681 agttctcaat gagtgcgaca tctattctta aaacgcgtag tttaatgaaa cgcttggaca
    10741 cagcgaaaat ggctgaactt gctgatcgtg cattgaaaga atgcgatatg atggaagaag
    10801 tcgttgaact tgttcatgaa tatgtaaaat aaacaacgta tcaaaagctg gtgtggcttg
    10861 taaaagagct acgccagttt ttttgttctt ttttaagaaa acgtttttgt cagtaagaat
    10921 agacaaaa
//
LOCUS       DAJSBL010000076        10634 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.078, whole genome shotgun sequence.
ACCESSION   DAJSBL010000076 DAJSBL010000000
VERSION     DAJSBL010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10634
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.078"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            23..646
                     /locus_tag="ONT85_002493"
     CDS             23..646
                     /locus_tag="ONT85_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2379437.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            1096..2145
                     /locus_tag="ONT85_002494"
     CDS             1096..2145
                     /locus_tag="ONT85_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR2379438.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            2142..3023
                     /locus_tag="ONT85_002495"
     CDS             2142..3023
                     /locus_tag="ONT85_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR2379439.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            3026..3796
                     /locus_tag="ONT85_002496"
     CDS             3026..3796
                     /locus_tag="ONT85_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR2379440.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            4075..4929
                     /gene="aroE"
                     /locus_tag="ONT85_002497"
     CDS             4075..4929
                     /gene="aroE"
                     /locus_tag="ONT85_002497"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HCR2379441.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            5107..6270
                     /locus_tag="ONT85_002498"
     CDS             5107..6270
                     /locus_tag="ONT85_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HCR2379442.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGVQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            6387..6797
                     /locus_tag="ONT85_002499"
     CDS             6387..6797
                     /locus_tag="ONT85_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCR2379443.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            6971..9199
                     /gene="topB"
                     /locus_tag="ONT85_002500"
     CDS             6971..9199
                     /gene="topB"
                     /locus_tag="ONT85_002500"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HCR2379444.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            9370..9654
                     /locus_tag="ONT85_002501"
     CDS             9370..9654
                     /locus_tag="ONT85_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2379445.1"
                     /translation="MGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAYSPTLILSTLTA
                     GAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQYFG"
     gene            9814..10050
                     /locus_tag="ONT85_002502"
     CDS             9814..10050
                     /locus_tag="ONT85_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HCR2379446.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            10047..10445
                     /locus_tag="ONT85_002503"
     CDS             10047..10445
                     /locus_tag="ONT85_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HCR2379447.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
BASE COUNT         3713 a         1772 c         2252 g         2897 t
ORIGIN      
        1 aggagaaaaa gtagaaaaga aggtgtttgt tatttgggaa gtagttaaat cgagagtaca
       61 agaaatgaat gaacatttct cagtgtcatt cgcagaaaat ccaagtaagt attttcgacg
      121 tcttgttttt ctgctcatct tatcattgct gttaacagtt gcaatgtttt tattgccgga
      181 aaaaatccga gaagagcaag taaatagtct cgtttattcg tccataccaa ccagcaaatt
      241 gaaacctttt gattacagca aagctgatca gaaaatcaaa caaagccggg cgatatcggt
      301 gatgttcagc aaaccgaacg ggaaaaccta tcaggacatg cttgatgttt ttaatgatcc
      361 tgagcttatg gaagaattga atcgaccgat cttttattat ccgatcgttt atgatgtaca
      421 tgaactggaa caaaaatata acatacgaac ggatgaagtg acatttattt tctttgatgg
      481 aggaaaagaa gcaaatcgga taactgcggg taagggagga ataactgatt ttgataagga
      541 gttgatccct gaactgaatc gacttccgtt agccaatatc aaacaattag aagaagaatt
      601 ggatagaacg ggagtaactt cccaaaattc cgagattgct aattgagaca ttggcgcgat
      661 gaaacggtgg gtctatatag cgctattttt aattatcaga acaagtgatg tttgatcttc
      721 aaaataacaa ataaaaaatt aaaaaatata gttgacgaaa ttcaaaaatt cgattagact
      781 taaatcaatt cttaaggaaa gggtgacctg taatgaactt atcaaaacgt atttcagcag
      841 aaaacaaaaa aaattggcgc agctggcgta gataagttgt attaatggtg agactttccc
      901 atagatacaa ctttttagag tgcatttcta aagcttgtat ctatgctaga catgaacatt
      961 gactgccgca tagattagaa tctatgcggt tttttgtgta aagacaatga ccgctttggc
     1021 cattgtcttt ttttgtagac tggatacttg cctgattata ataaataaaa agattgactt
     1081 tgcaggagga aaaaaatgaa aaacaaacga ttaataacag ttgtagtaat tattttagtt
     1141 tatttagcag gtgcatttat ttatggaaaa gcctcaggcg aaaaagaaag tacttcgacg
     1201 aaccagacaa aaaaaacaac agttgttggg gtattgcaat acgtgagcca tccggcatta
     1261 gatgagattt atcgaggaat caaagatggt ctgaaagaag aaggattaaa agaaggagaa
     1321 aatcttgaga tcaaatttca aaatggacaa gccgatcaga gcaagctagc cacaatgagt
     1381 caacagctag tacaatctga tccagatgcc ttgattggaa tcgcaacacc tgccgctcaa
     1441 tcattagcaa acacgacttc atcgatccca gttgtgttag gtgctgtaac ggatcctgtg
     1501 ggggctggat tagtcgcttc tttagagaaa cctggtggta atgtaacagg tgtttctgat
     1561 cagccgcctg ttacttcaca aatcaagctt gcttctgatt tgttgccaaa tgcaaaaaaa
     1621 gtcggaatct tgtatgcttc ttcagaggat aattcaaaat accaagtagc acaagcagaa
     1681 gaagctgcaa aaaaagaagg gttagaatca gtaaaatatg cggtgccttc cactaatgag
     1741 attgcacaaa cggttcaagt gatgagccga caagtcgatt ttatctatgt tccattagat
     1801 aataccattg ccaatgcgat gcaaacagtg gtaaaagaag caaacaaagc aaatatcccc
     1861 gtcattccat ctgtggatac aatggtggaa caaggtggac tagcaacaat cgggatcaat
     1921 caatatcaat tagggcttca aagcggtaag atggcggcag aacttgcgag tggaaaagaa
     1981 aaaccggaaa cgacgcctgt ttatacattt gatcaagggg atacagtgat caatcaatct
     2041 caagctgatc gcttaggaat cattgttccc caatcgatga aggaaaaagc caaaatcatt
     2101 acagatgact cgcagcaaga aacatctaag gaggatgaca aatgatcgta tcagctattt
     2161 cacaaggcat gctttgggca atattaggat tagggatttt catgacttat cggatcttag
     2221 actttcctga tatgacgaca gaagggtcct tcccattagg tgctgctgtc tgtgtgacag
     2281 ctattactca tggggtagcg ccgattgtag ctacattatt aggcgtaggt gctggcatga
     2341 tagcaggact cgttacgggg ttactttata caaaaggaaa gataccggtt attttagcag
     2401 ggattttagt gatgtcagga ttgaactcag ttattttgtt tgtgatgcga acaccaaatc
     2461 tttctttatt gaatcatccg attctacaag acagcttcca tcgctttggg ttacctgatt
     2521 actttgacac agtccttctt ggcttcatct ctttgggcat cttgattgct gtactgctat
     2581 tcttcttcaa tacagatttg ggtcaaggct atattgcgac aggagacaac gaaacgatgg
     2641 ctcggtcgtt agggataaaa accgatcgta tgaaaatttt aggtttaact ttgtcgaacg
     2701 gtgtcattgc attatccggt gcgctgattg cccagaacga cggatatgca gatgtaaata
     2761 aagggattgg cgtaatcgtc atcggtcttg cttccatcat catcggtgaa gtgatcttcc
     2821 aagaattgac gctagcagaa agattgattg ctattgttgt cggcagtatc atttatcagc
     2881 tattgatttt agtagtaatc aaactagggt ttgacacgac ttatctgaag ttgttctccg
     2941 ctatcatctt agctatttgt ctgatgatcc cgcaattgaa gaaagcactg aacttaaaaa
     3001 cgggttttga gaaggaggca tagtaatgac tgatatattg acaatcaaaa atggcagaaa
     3061 agtcgtaaac aatggaatga atgaagagaa agtactattg gatgaaatta accttacaat
     3121 cagagaaggg gacttcatca ctgtccttgg cgggaatggt gctggaaaaa gcacactgtt
     3181 caatacgatc gctggtacgc ttcaactcag tcaaggatcg atctctttta aagatcagct
     3241 gatcacaaaa gattcagaag aaaaaagagc acgattcctt tctcgagttt tccaagaccc
     3301 caagatggga acggctcctc ggatgacagt agcagaaaat ttgctacttg cacaaaaaag
     3361 aggtcaaaaa cggaaattcc gtttacgtaa cttgaaaaat caaaaagaag attttttcag
     3421 actatgtcaa gaagtcggta atggcctaga gcaacacttg gatactccag cgggtgaatt
     3481 atctggtggt caacgccaag cattaagctt gctgatggcg acgatcacag ctcctgagtt
     3541 actattatta gatgaacata ctgcagcttt agatcctaaa acagctaaag cattaatgcg
     3601 attaacaaat cagcggatca atgaacaaaa attgacttgt ctcatgatca cgcatcgaat
     3661 ggaagacgcc ttgaaatacg gaaatcgtct gatcgtactc caaaaaggga aaatcgttca
     3721 agatatttct gaaaaagaaa agcaacattt gacaatgggc gaccttttgc agttctttga
     3781 agaagtagtg gattgatttc gacagaaaaa ttcctttcag tagaaggact tgaaaactca
     3841 ttgacatctt cctttgattc atcctatact taaaacaata aacaattaaa catatttggg
     3901 tgttttgtag gatcacgtag tcaatgattt taacaattaa atagaatatt ctttgctctg
     3961 acaagtaaac gatgagaaaa gaatttgcta ctttgccacc tagacgtttc gtactttttc
     4021 gtatcaaacg ctaagtggct ttttttattg aagtaagaac aaaggaggaa taagatgatt
     4081 tctggacaaa ccaaattagc tgggctcttt gctgatccgg cttctcacag tttatcccca
     4141 ttgatgcaca atactgcttt tgaagcaaac aacatcgatg cggtgtattt agctttccaa
     4201 gtaaatccaa tgaatttgaa gcaagcagtc gaaagtattc gaacttttga tatgttaggt
     4261 gtaaatcttt cgatgccgaa taaaacagcg gttattcctt atctggatga ggtaagccag
     4321 gaagcgcaat tgatcggagc agtgaatacg atcgtgcacg aaaagggtcg tttgattggc
     4381 tataataccg atggtgcggg cttcatgcaa tccgtgcaag aagctgggat ttcgatcaaa
     4441 aagaaaaaaa tgactctact gggagctgga ggtgcagcaa aggcagtcac tgttcaagct
     4501 gcgttagacg gcgctgagga aatcgtagtt tataaacgaa acaaccaaac attcgctcaa
     4561 gtaaaagaaa cattcgaaca actcagctat cgaacttctt gtaagataaa agttcgtgac
     4621 tttgccgata cgcatcaatt aagaaaagac ttgaaagaaa gctgtcttct agtcaatgca
     4681 acagatatcg ggatgggaaa gaaaatcaat cagtcaccgc tcttagatat aagcatgatg
     4741 catgagaatc tagcggtttt tgatttgatt tatgcaccta gagaaacacg tttattaaaa
     4801 gaagcaaaaa aagtaggagc aaaaggatac aatggacttg gcatgttgat ctaccaagga
     4861 gcaatcgcgt ttgaattatg gacaaaggaa aagatgccga tccatgtgat tgaaaagctg
     4921 tttgattaaa aaaggataat agataaagat cttttattct cgcataaacg gtctcaagaa
     4981 aagtaatcct ttatgtatcg aagcatttat ttcgttattt tttattaaat tatcaaaaaa
     5041 tgataggctt ttgtcgattg gttatcgtat cctttattta tcagtaagaa acgaggagga
     5101 accacaatga gtcggatgga tcgctataag gacatccata agaaggcgaa acctctgaag
     5161 cgggatacgg ggagtatttt tgctagaaga gaacgaaaac gaacgcgtat ggaaaatgag
     5221 gaagatgcta cccgtattta tgaaagagag cagcattcat ttgaacattt gaaccaatac
     5281 gagcaacaga tatacagaga tgcaccaaga gcagaagaac aacaaaaaaa taaaaaattt
     5341 ttcaatcggc aaaagaagaa caaaccgtta aaacctcgaa gaaagcgatc gtggaaaaaa
     5401 atcattctat cgattttgct tgttttgatc ctttattcag gtatctcatt ttttattggt
     5461 aaatatgtag ctgaacatga cacaagctta gaggcagcga cttcagaaac ctttcatggg
     5521 caaaaagcga cgaatggtgc gcataatatt ttgatccttg gaagtgatac acgcggagaa
     5581 gatgccgggc gagccgatac gatcatggtc ttacagttgg atggtccttc ccataagccg
     5641 aaactgattt cttttatgcg tgacagcttc gttacgatcc caggggtagg acaaaataag
     5701 atcaattcag cttatgcata cggaggagcc gatctagtgc gtcaaacttt agtagaaaat
     5761 tttggaatcg attgtcaata ctatgcgaaa gtcgatttca aatcgtttga aaaagtgatc
     5821 gatgccttat tcatgaacgg agtgaagatc gatgcagaaa aagatctgaa tctggatggt
     5881 gtagatatta aaaaaggggt ccagaagatg gatggacatg ttcttttgca gtacgcacgt
     5941 ttccgaatgg atgaacaagg agactttggc cgtgtcagac gccaacagca agtaatgaac
     6001 gctattttca gccaattgaa gaatccattg aatctgatcc gtgcacctta tgcagctggt
     6061 aaagctttag gatatacatc cacagatgtc actgcttggt tcttgacgaa aaacctcttt
     6121 tcgattgcac gaggagctgg tggcattgat cgaataagtg tgccagtaga cggttcgtgg
     6181 aattatgggc aaagtgatta tgctggcagt attcttgaaa ttgataatca aatgaatcaa
     6241 tctgctatta cagatttttt gaataaatga tcataaaaaa gatcaagaag aagctgcgca
     6301 aggcttttgt catgaatcaa tcaaacgatt cactcgtaaa aaacgctttc ttatggtaga
     6361 tttaaccaag agaggagctg tttacattgg atatctatct gaacttcaaa aatgagtcgc
     6421 gtgaagccat cgctttttat gaaaaagttt ttcagactga agcatcaggt atcaccacat
     6481 acggtgaaat gcctggaata gaacaaatga acttgccaga agaagcaaaa aatctaatca
     6541 cgaatgcacg gctgaacatt tatgatacat tagttatgtt tgcagatacg ccagaaggaa
     6601 ttggtcctga attagttgtg ggaaataatg tttctttagt catccagaca agaaatgaag
     6661 aagaaacgaa gcgcctattt gaaagcttag ctgagggtgg taagattgag atgccactgg
     6721 atgctgttcc gtgggctcag ttgtatggga tggtaactga tcgatttggc atcaactggc
     6781 agattaacca agagtgacag ctaaagatga atattttctg gaaacatcaa gcagcaaacg
     6841 gatcatcgtt tgtttgcttg atgtttttgt gtctatttcg actatacggt atcttttttt
     6901 tattgccaga tgcgaaaata tgttccataa tagagaagtc cgcaaaaaat taagtaacgg
     6961 aggtcagaag atgaagacgg tgatactagc tgaaaaacct tcacaagcaa aagcctatgc
     7021 cgaaaccttc caacagcaaa agcggcatga agggtattat gaaatcagcg atccattgtt
     7081 tgctggtgaa gtaaccatca cttatggttt tggacatttg gtagatatgg ttgctccagg
     7141 agcttatgaa gagcgttggg caaaatggtc tttagaaaat ttgcctattt tcccagaaac
     7201 cttccgttac gaagttccaa tggacaaacg tgcacaattt tcgatcgtaa aacgagaatt
     7261 gcagtcagcc gataccatca ttattgcaac agatggtgat cgagaaggtg aagccatcgc
     7321 gtggtcgatt attcgacaag caaacgcatt ttctaaggaa aagaactatt tacgtttatg
     7381 gatcaattct cttgaaaaag aagcaatcta tgaaggtttt aagaatctcc aacctggaaa
     7441 gaactatttt ccaaaatata aagaagcgca agcaaggcag aacgctgatt ggctgattgg
     7501 aatgaatgga agtccattat atagcttgct acttcaacga aaagggattg atggcagttt
     7561 ttctcttggt cgtgtacaaa caccgacact ctatatgatt tatcagctgc aagaagaaat
     7621 caaacacttc aaaaaagaaa cgtactttga aggagaaggt ttaattacga caaacaaagg
     7681 gcagttttct ggaaaaatcg taccaaaaaa agcatttaaa acccaagaag agttaacgaa
     7741 ggaaatagaa aaacttggag cgcatttagg taagcaaaac ggacggattc ttgaagttac
     7801 gaaaaaggaa aagaggttgc atagtccacg tttattttcg ctatctagtt tgcagacaaa
     7861 gatgaatcag ctaatgaaag caagtgccaa agctacactt gatgctgctc aaggattgta
     7921 cgaaagcaag tttctaagct atccaagaac ggattccttt tacattacag aaaatgagca
     7981 tcagtatctg gttaaacatc tgcaaaatta taaaaaattt ttaggaatag aagaagtaca
     8041 gacaactgag atcaaagcaa agaaacgata tgtcgatgcg aaaaaagttc aagaacatca
     8101 tgcaattatt ccgacaaaga cgattccgac tcctgcacgc tttgcgaagc tttccaaatt
     8161 acagcaagca atctatttgc aagttttgcg cactactgtg gctatgtttg ctgcagatta
     8221 tatctacgaa gagacgaccg taatgacggg cgtacagcaa ttaaagctaa aaagcatcgg
     8281 aaagatccca ttacagcaag gttggcagga aatcttacgc atcaagtcaa aaaagaaaga
     8341 agctcagact cttcctccta tcgtagaaaa tgaagaagca gaagtagaac tgaaaagtat
     8401 ggagaaagaa acacaacctc caaaacccta taccgaagga acgttgatca cagcaatgaa
     8461 aactgcggga aaaacgctgg ataatgatga agcgcaagaa attctcaaag aagtagaagg
     8521 aatagggaca gaagctacac gagccaatat cattgaaaat cttaagcaga agcattacat
     8581 cgaagtcaat aaaaacgaaa taactgttac gccaaaagga atcactcttt gtaaggcagt
     8641 agccaatgaa ccattactga cgagcgcaga aatgactgcg cgctgggaag ggtacttgag
     8701 aaaaattggt aaacaagagg ggacaccatc tgttttttta gagaatatca aaaaattcat
     8761 cctacattta ttggcagaag ttcctaatca aatcgacaag gtagactttt cagatgagat
     8821 tagcgggaca cgtcagataa aagcgatgga gaagaaaaac gatcaattag gaagatgtcc
     8881 aaaatgtaaa aagggaatag tcatgcttta tcctaaaatc gctacatgct tgaatccgga
     8941 atgcgacttc aaattgtggc caacagtagc taaaaagaag ctgactaaaa cgaatctcag
     9001 agatttgctt tcaaaaggaa agacaagtaa agttgtcaaa ggttttactg gtaaaaaagg
     9061 gaaattcgat gcagttttag tattaaaaga agatatgaca atcggttttt cttttccatg
     9121 gatcgaaaac agtggaacaa aagcagttga tggaaacaaa gaaacatcaa gaaaaaataa
     9181 tgaaaacgaa gtagagtaaa tgaaaacaat gaaatagcgg aatatttatt ccgaaaagtg
     9241 agaatataaa taccaataaa actaaaaaat atcagtgaat caagcaaaaa cgttggggta
     9301 ggctgatttt atatagatag caaggagttt ttttatttga aaaaagtgca ttcattttta
     9361 aattatctta tgggagcaat cacgattgtg gctgtgtacc aagcatttct aaagtatcct
     9421 gattacggga cgaagttgtt gtcattgacg cacaatcaaa cagcatattc accaacgttg
     9481 attttatcga ctttgactgc aggagctaaa agcgctactt atttttttca taaaacgaca
     9541 agcatgctaa agcgaaagaa acagcgaaaa caattgttgg tggtggtagc ggctatgatc
     9601 attatttatt ttctaccagt tattacacaa gtatttcatc aatattttgg ttaagaatac
     9661 gagagtttgt gacaaaagtc atgtcacaaa cttttttctt tgtcattttt tcgtaagaaa
     9721 aagcaaaata aaagtatata cattgattaa aagtatatac ttggatataa tgtacttact
     9781 atagaatgag aatggtatag agggtggcga aatatgaaaa aaatgaaaaa gtataaaatt
     9841 agaaaatctg gcaatagcga tgtaacaact atcccaccag aagtaaaaga attcatgggt
     9901 gtgcagacag gtgacgcaat cagttatgtt tttcaatctg atggttctgt acgaatgatc
     9961 aaagcacaag aagaacctga tattgattca ttagttgatt cgatcatgaa tcaatatgaa
    10021 gacgcgttaa aagatttggt tgatttatga ggtatctttc gactaaagaa attatcaaaa
    10081 tcaacgcgaa agttatttta cgtcactcgc caggcgaaat gatcggtgta aaagatgcaa
    10141 atgcattaga tatggccgtc aaacagcctt cacaagcagt attcaatcaa gaactctatc
    10201 ctgaaatcta tgaaaaagcg gcgattttag caattaattt agctaaaaaa catccttttc
    10261 ataatggcaa taaacgaact gctcttgttg ccatgttgtt gtttcttcaa ttgaataatt
    10321 gtccagttgc tttttctcgt caagaagctg ttgattttat cataatgatt accactagtt
    10381 cattggattt cgattcacta aagtccaaga taaccagtta tttacgacaa accgcaataa
    10441 aatgaacgaa ttaaaaactt tatttctcaa ttagatcagc atgtcattaa ggaatatgct
    10501 gattttttta tgtttaatca tacaaaatag ttttttcata aaaatatgga aaatatggat
    10561 taattttgaa ataattgatt taaaaggaat acaaaagagt gaatagtagg aaatagtggt
    10621 ttagaactag tggg
//
LOCUS       DAJSBL010000077        10100 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.079, whole genome shotgun sequence.
ACCESSION   DAJSBL010000077 DAJSBL010000000
VERSION     DAJSBL010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10100
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.079"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            278..787
                     /locus_tag="ONT85_002504"
     CDS             278..787
                     /locus_tag="ONT85_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCR2379448.1"
                     /translation="MVQGIERGVYVVDKIQFNQVIEEFIKVNNLMTHIQKRPIRITND
                     LKLSTSMIHLIDVIGNYPETSITELADRLGVTKGAVSQQIPTLEKMNLISISQKKGNK
                     KTKLVSLTKYGRKVYESHNYLHEELYSSINEALESFSPDQISTIKQILEKVSYTINEY
                     QVSLTRKED"
     gene            880..2042
                     /locus_tag="ONT85_002505"
     CDS             join(880..1156,1156..2042)
                     /locus_tag="ONT85_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCR2379449.1"
                     /translation="MSRRQRRTYSKEFKQQVVDLYLAGKPRAEIIRDYELTTSAFDKW
                     VKQAQTTGSFQEKDNLIPEQVELIALRKKNKQLEMENDILKQAALILGPKRQVIDTNK
                     HKYSISAMCKVLNISCQTYYYQAKPVEDESELEELVQEEFIRNRKAYGTRKLKQCLAN
                     LGLQVSQRRIGRMMKQRGLRSTYTLAHFKFHSSTCNEAKTANILDGTFLQEKSLEAIV
                     TDLTYVRVGKKWQYICLILDLFNREIIGYSCGEKKDATLVKEAFAKIPYSLNNVQLFH
                     TDRGKEFDNQTIDEILWAFNITRSLSKKGCPYDNAVAESTYKSVKVEFVHQYRFETLA
                     NLRLELFDYVHWWNTIRLHGTLGYETPISFRQQRLAQRILDNELGCDTGGEAP"
     gene            2322..3302
                     /locus_tag="ONT85_002506"
     CDS             2322..3302
                     /locus_tag="ONT85_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR2379450.1"
                     /translation="MKKIGIREVAKEANVSPTTVSRVLNDRGYLSEETKRKVFDAMKK
                     LEYYPNELARALFKNETYTVGLIFPTITNPFHAELIQDIEFELSSRGYKVLLCNSLNN
                     PTKEKEYLTMLRKNQVDGIIVGTHNKDIADYDIPGLPIVAIDRSLGENTVTVSCDNYR
                     GGELATQLLVNHGCQNILCIRGDSKIKLPANSRTLAYKDVLNKFMLEPLVLEVPFVKE
                     INEKNEIIKTYLETHPNIDGVFAGDDLLASLAINNLGSSGKRVPKEVKVIGFDGAQQT
                     LIYNPELSTIQQPIDQISKVAVKKLLNMIKGEKETDELSLPVKLIKKNTA"
     gene            3424..4842
                     /locus_tag="ONT85_002507"
     CDS             3424..4842
                     /locus_tag="ONT85_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HCR2379451.1"
                     /translation="MEKLLIKSNNHQLYYQPKDVWVGDVMPYGKEGKFFIYHQRDTRN
                     PVPFGEPFGWALATTKDFVHFEDFGESLLRGDDNERDQFIYAGSVFEANGKVHAFYTG
                     YNREFLAQGKTSQVLLHAESDDFVHWEKSETALELEPQEGYDIRNWRDPFVVWDDEKE
                     EYLLILGARKGQDKHLQTGRLVEFTSKDLTNWEFKGDFWAPGLYTMFEMPELFKIGDW
                     WYLVYSEYSDGNKILYRMSQSLEGPWIKPKDEAFDGRAYYAGRTAYDGKRRVLFGWVP
                     TKDQENDKNNYLWGGTLVPHEVYQKEDFSLGVKPIDEVWNAFINLHDIADVEIETADT
                     REEVTLVKNSGTLFNLETTISFDENLSDFSLRLYKNLETDESYEYRFNLDEGRLTFDK
                     NPCYPWFQYMDKGLIRPISLKPNQSYKLKVIVDDNIMTLYIDGIALNARVHGCFGQAI
                     TITATNGSMTAINTKFSNEIKK"
     gene            4913..6382
                     /locus_tag="ONT85_002508"
     CDS             4913..6382
                     /locus_tag="ONT85_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCR2379452.1"
                     /translation="MDYKKIAEDVLENVGGKENISSATHCITRLRLILKDKSKANMEM
                     LEEIEGAKGVLFNSGQLQVIFGTGIIEKVYKEFVELTGAKEVSVSEMKDEGVNNSGNR
                     LQRAFKVFSDIFIPIIPAFIGAAMVLGIRSLITTPGMFGMDGSFADHNVFLNDLSKFL
                     GIIATTFDYLPVLVMYSATKRFGGNPILGLLVGFVMIHPGLANRNDFVMGTIKPDYWH
                     LFGLSIPAVAFQGGVFPAILTSWFLAKVEKITNKYSPQVLAYILVPTVTIVLANFALF
                     LVFGPLGNFIGNVLGGIIDFLYMKMGAFGAFVFAGALQPLVVTGTQHAIQGIEANLVA
                     TTGFNYIQPIWSVSIIAQGGGCIGMYLLFKKKSKDRDIAISSFIPTLVGITEPAIFAV
                     NLKYSIVPFVCSFIGAGFGGAFMKIFDVKAIGQGLTVLPGLTIANPVLPYIFGNLIAF
                     ILPIIFILAYDKYKVFIPEAKHRKFAEKTDSKKLPVQVK"
     gene            6392..6859
                     /locus_tag="ONT85_002509"
     CDS             6392..6859
                     /locus_tag="ONT85_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCR2379453.1"
                     /translation="MLNFFRKNEISVVAPATGVFEKLEKLSDPVFSKGMMGQGFAIDA
                     KNETIVSPIDGFVSSIFPTKHALGIKTKSGIEILVHVGIDTVELNGEGFDIKVQEGQK
                     VKAGDILMTINFVVFEKNHKTKDVIVVFPDYQDEIFIPKLEEVKQGQELFVLG"
     gene            6895..7767
                     /locus_tag="ONT85_002510"
     CDS             6895..7767
                     /locus_tag="ONT85_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HCR2379454.1"
                     /translation="MVFGAIEAGGTKFVCAIGDKDLNIFEKVSFPTTTPKETMNLVLD
                     FFNHHKDDLEGIGVGSFGPIDIHCESKTYGFITSTPKLAWQDFDFIGSLKKQLNIPTA
                     WTTDVNASCYGEYVSGNGRGLSSVVYYTVGTGIGGGAIQNGNFIEGFSHPEMGHMLVK
                     RHNKDNFSGTCPFHGDCLEGLAAGPAIERRSGKKGQEVTENDVTWEIEAYYIAQCAYN
                     TTLMLSPDVIIFGGGVMKQKHLKKKVQDKFIELINGYVAIPDVDNYIMTPKLLDNSGI
                     VGCLALARDVKEKS"
     gene            7848..>10100
                     /locus_tag="ONT85_002511"
     CDS             7848..>10100
                     /locus_tag="ONT85_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HCR2379455.1"
                     /translation="MKKKMKDEVLEYIKNNLKYYDFSAQDIAMKFCIKRNVASHYLNQ
                     LFSDGKLLKNDSVRPVMFKYNQQKPKDCFSKFIGADISLKSTIDKCKATVMYPPNGLP
                     LIIKGNSGVGKSFLASLIYQYALDRKVIHNDAKFVVVNCADYANNPELLSAVLFGYKK
                     GAFTGAEKDTAGMLSSANGGYLFLDEVHNLSAENQEKLFLLMDSQKYRKLGESVQWEY
                     ANVRLILATTEDKNTSLLATFRRRIPAEITLPDYNNRSTSEKIQLLFEFLKDEAITID
                     SKIFCSIELFTDLLSKTFEGNVGELRNEIKLLCAEGYLGNKRKSSIYLGDKLDSGFWI
                     DPEISIDLKKMFLKSLSEIDLIELFQNHISIEESMSQLRDRILKECAGSSEYNYLESD
                     EFIALRNYVVNKISPIIDSTGLCLLDEFLADISLFIMFIDVIEETSRNFLLSKFRKFR
                     MKNDKQKLLANEIWSSLEVEESKQELLLKWILFLVKKFYVKIPETHCLIVMHGKITAS
                     AIARETNKLLNTYVYEAFDMPIEGETSDLINLINNFCKSIDTTNGLILLVDMGSLEQM
                     YEKIESNVIGDLVILNNVSTALAIECGIQVCQKKPISHFYQMDFDSFQVKVQYYKGLS
                     QKKNVIVSCLSGEGISEKVKDILKRYLDNQVEILIFDFNALKKIAKEKDTMIFKNTIC
                     VLSTTEFYIQGIDCLNLENLINGNQTLEKLNKYMDSDSCEFCLNELVKLFTIEGASTK
                     LRFLDSKKVFN"
BASE COUNT         3503 a         1462 c         2008 g         3127 t
ORIGIN      
        1 ttttttattt gtctcgctta attatacaaa cggcgtgata aaaaacgaga aatgaattat
       61 aggtttagac ctttatcatt tctcgtttta ttttattgta tcaaggctct atcataagtg
      121 gtaaaatcta cttttaaatc acatgtaatc attgataagt ccagtgtttt agcggataat
      181 cagcttttta tttatttttt cttataaatt tgataaatag aaattagtat agccctaaac
      241 aaattatttg tgttttattg tttagggcta tactatattg gtacaaggaa tagaaagagg
      301 tgtttatgtg gttgataaaa ttcagtttaa tcaagttata gaagaattca tcaaagtaaa
      361 taatttaatg acccatattc aaaaacgacc aatcagaata actaatgatc taaaactcag
      421 tacgagtatg attcatttga ttgatgtgat tggaaattat cctgaaacta gtattacgga
      481 attagctgat cgattaggcg ttaccaaagg agctgtatcc caacagattc ccactttaga
      541 gaaaatgaat cttataagca tttctcaaaa aaaaggaaat aagaaaacta aacttgtatc
      601 gttaacaaaa tatggtcgaa aggtgtacga atcacataat tatttacatg aagagcttta
      661 tagctcaata aatgaagcgt tagaatcatt ctcaccagac caaatttcaa ccattaaaca
      721 gattctagaa aaagtatctt atacaatcaa tgaatatcaa gtaagtctga caagaaagga
      781 agattgacat gaaaaaaatg atttttgaat cggctacact agactagaca gaaaaaataa
      841 ggtgtgtaaa ataaatgaaa acacactgga ggaattttta tgtcaagacg tcaacgtaga
      901 acctattcaa aagaattcaa acaacaagtc gtcgatcttt atctcgcagg aaagccccga
      961 gctgaaatca ttcgagatta tgagctgact acctcggcct ttgataagtg ggtcaagcaa
     1021 gcgcaaacta ctggctcgtt tcaggaaaaa gataacttga tccctgaaca agtagaacta
     1081 attgcattac gaaagaaaaa taaacagctt gaaatggaga atgacatttt aaagcaagcg
     1141 gcgctgatat tgggacgaaa agacaagtaa ttgataccaa taagcacaaa tactcaatat
     1201 cagcgatgtg taaagtgtta aacatctctt gccaaaccta ttattatcaa gccaaaccag
     1261 ttgaagatga atcagaactg gaagaactcg ttcaagaaga atttattcgc aatcgaaaag
     1321 cttatggtac caggaagctg aagcagtgct tggccaatct aggtcttcaa gtgagtcaac
     1381 gcagaatcgg ccgtatgatg aaacagcgtg gcttgcgttc aacatacaca ttggctcatt
     1441 tcaaattcca ttcttctact tgtaacgaag ccaagaccgc caatattcta gacggaacct
     1501 tcctacagga aaagtcatta gaagcgattg taaccgacct tacctatgtt cgtgttggga
     1561 agaaatggca gtatatctgc ctgattcttg atttgtttaa tcgagaaatc atcggctatt
     1621 catgcgggga gaagaaagat gcgacgttag tcaaggaagc ttttgcaaag attccgtact
     1681 cgttgaataa tgtacaactt tttcacacgg atcgtggaaa agaattcgat aaccagacga
     1741 ttgacgaaat tttatgggcc tttaatatca cgcgttcctt aagcaaaaaa ggctgcccct
     1801 atgataacgc cgttgcagaa tccacctata aatctgtcaa agtagaattc gttcatcaat
     1861 accgctttga gacattggct aatttgcgat tagagctatt tgattacgtc cattggtgga
     1921 ataccatccg gctgcacggc acgttaggct atgagacacc gatcagtttt cgacagcaga
     1981 gattggcgca gcgaatcctt gataatgagc tcggatgtga taccggtggc gaggcacctt
     2041 aattatttga atgagcttct gccttagaga atcagattcg aggaggctcg ttgtcaaggg
     2101 cagtcggaac accagcagca agcttacgca ataccttata atttttgtca aaaaaagtgt
     2161 tgccattcca catatatctt taaacagcta ttgccagata aaaataaatg atattatata
     2221 tataaatact tatccgaaaa ggggaatgag agagtcaagg ttcttggaat gtcgaatagt
     2281 cgagtaggta gattttctat ctacaacctt gttgtttaaa aatgaaaaaa attggaatta
     2341 gagaagttgc gaaagaggca aatgtttctc ctacgactgt gtcgagggta ttgaatgatc
     2401 gtggatattt gagtgaggaa acaaaaagaa aagtttttga tgccatgaaa aaattagaat
     2461 actatcctaa tgaactggcg agagcacttt ttaaaaacga aacatatacc gtaggtttga
     2521 tttttcctac aattactaat ccatttcacg ctgagttaat tcaagatatc gagttcgagc
     2581 tttccagcag gggatataaa gtgttgctgt gcaatagctt aaacaatcca acaaaagaaa
     2641 aagagtactt gacgatgttg agaaagaatc aggttgatgg aattatagta ggaacgcaca
     2701 ataaagatat tgcggattat gatattccag ggctacctat tgtagcgatt gatagaagtc
     2761 ttggtgaaaa tacagttaca gtatcttgtg ataattacag gggtggggaa ttagctactc
     2821 aactacttgt aaatcatggc tgtcagaata tactgtgtat tcggggagat agtaagataa
     2881 agctacccgc gaatagtagg actttagcat ataaagatgt tttgaataaa ttcatgctgg
     2941 aaccgttagt cctagaagta ccatttgtaa aagaaatcaa tgaaaaaaat gaaataatta
     3001 agacatatct ggaaactcat ccaaatattg acggggtttt tgcaggagat gatttattgg
     3061 cttctttggc gattaacaac ttaggaagtt cgggcaaaag agttccaaaa gaggttaaag
     3121 tgattggatt tgatggagct caacaaacat tgatttataa tccagaatta tcaactattc
     3181 aacagcctat agatcaaata tctaaagtag cagtaaaaaa attattgaat atgattaagg
     3241 gcgaaaagga gacggatgaa ctttcacttc cagtaaagct cataaaaaaa aatacagcat
     3301 aagttaaatc ggcgaaaaat tccttcgtcg atttaaaaat atttttatga aacgggttga
     3361 catatgaaac gggttgacat atattagttg tgaagataaa aaccttttca ctggaggaag
     3421 aaaatggaga aattattaat taaaagcaat aatcatcaac tttactacca acctaaggat
     3481 gtttgggtcg gagatgttat gccttacggg aaagaaggaa aattctttat ttatcatcaa
     3541 cgtgatacta gaaatccagt tccttttgga gaaccgtttg gctgggcttt agcaactacg
     3601 aaagattttg ttcattttga agactttggg gaatctcttt taagagggga tgacaatgag
     3661 agagaccagt ttatctacgc tggttctgta tttgaggcaa atggaaaggt tcacgcattt
     3721 tatacagggt ataatcgtga attcttagcg cagggaaaga catctcaagt gttgcttcat
     3781 gctgagagtg atgattttgt tcattgggaa aaatcagaaa cagcattgga gttagaacca
     3841 caagaagggt atgatattcg aaactggaga gatccatttg tcgtttggga tgatgaaaaa
     3901 gaagaatatt tgctaatttt aggggcgcga aagggtcagg ataaacatct acaaaccgga
     3961 cgtttagtgg aatttacatc aaaagattta actaattggg aattcaaagg tgatttttgg
     4021 gctccgggat tgtataccat gtttgaaatg ccagaacttt tcaagattgg tgactggtgg
     4081 tatcttgtct attcagaata ctcagatgga aataaaatac tctatcgaat gagtcaaagt
     4141 ttggaaggtc cttggattaa gcctaaagat gaagcttttg atggacgggc ttattatgca
     4201 ggtagaacag cttatgatgg aaaacgtcga gtcctatttg gatgggtacc aacaaaagat
     4261 caagaaaatg ataaaaacaa ttatttatgg ggaggtactt tggttcctca tgaagtctat
     4321 caaaaagagg atttttcact tggggtaaaa cccatagacg aagtgtggaa tgcattcata
     4381 aatttacatg atattgccga tgtagagata gaaacagcag acacaagaga agaagtaacg
     4441 cttgtcaaaa atagtggaac tttgtttaat ttagaaacaa caatttcatt tgatgagaat
     4501 cttagtgact tttcactgag attatataaa aaccttgaga cagatgaatc ttacgaatat
     4561 cgcttcaatt tagatgaggg gcgtcttaca tttgataaaa acccttgtta cccttggttt
     4621 caatatatgg ataaaggact tattagacct atatctttaa agcctaacca atcttataaa
     4681 ctaaaagtga ttgttgatga caatataatg acactttata ttgatggaat cgctcttaac
     4741 gctcgtgtac atggttgttt tggtcaagca attactatta cagcaacaaa tggatcaatg
     4801 actgcaataa ataccaagtt ttcaaatgaa ataaagaaat aataaacgtc aatcatgtgg
     4861 caggtctgaa aatcaaacat gacctgtcac atttaaaagg gaggataaat gaatggacta
     4921 caaaaaaatt gccgaagatg tattggaaaa cgttggagga aaagagaata tctcaagcgc
     4981 tacacactgc attactcgtt tacgtttaat tcttaaagac aaaagcaaag cgaatatgga
     5041 aatgcttgag gaaatagaag gtgcaaaagg tgttcttttc aatagtggac agttacaagt
     5101 aattttcgga acaggtataa ttgaaaaggt ttacaaagag ttcgttgaat tgacaggagc
     5161 taaggaagta tccgtttctg aaatgaaaga cgaaggagtc aataattcag gaaatagact
     5221 tcaacgagct tttaaagttt tctcggatat ctttattccg atcattccag cttttattgg
     5281 ggcagcaatg gttttaggaa ttcgttcgtt gattaccact cctggaatgt ttggaatgga
     5341 tggctcattc gctgatcaca atgtattcct aaatgattta tccaaatttc tgggtattat
     5401 tgcaaccaca tttgattatt taccagtttt agttatgtat tctgcaacaa aacgttttgg
     5461 cggaaaccct attttaggat tacttgtagg atttgttatg attcatccag gactagccaa
     5521 tcggaatgat ttcgtcatgg gaactataaa gcctgattat tggcatttat ttggattaag
     5581 cattccagca gttgcgtttc aagggggtgt ttttccagca attttgactt cttggttttt
     5641 agcaaaagtt gagaaaatta caaataaata ttctcctcaa gtacttgctt acattttagt
     5701 cccaactgtg acaattgttc ttgccaactt tgcactattt ttagtgtttg gtccattggg
     5761 gaattttatc ggtaatgttt taggtggaat cattgatttc ctatatatga agatgggtgc
     5821 ctttggtgct tttgtttttg caggcgcgtt acaaccatta gttgtaacag gaacacaaca
     5881 tgctattcaa ggtattgagg caaacttagt cgcaacaact ggattcaatt atatccaacc
     5941 tatttggtca gtttcaatta ttgcccaagg tggcggttgt attggtatgt atttactctt
     6001 taagaaaaaa tctaaagata gagatattgc gatttcaagc tttattccaa cactggtagg
     6061 tattactgaa ccggctatct ttgctgttaa tttgaaatat tcaatcgttc cttttgtttg
     6121 ctcgtttatt ggtgctggtt ttggtggagc atttatgaaa atttttgatg taaaagccat
     6181 tggacaagga ttgactgtat tgccaggtct gactattgct aatccagttc ttccttatat
     6241 atttggtaat ctcatagcat tcattttacc aatcattttt attctcgctt atgataaata
     6301 taaagtgttt atccctgaag ctaaacaccg taaatttgca gaaaaaactg actcaaaaaa
     6361 attgccagtt caagtaaagt gagatgagag catgttaaat ttttttagaa aaaatgaaat
     6421 aagcgttgta gcacctgcaa ccggtgtttt tgaaaaactg gagaaattaa gtgatccagt
     6481 cttttcaaaa ggaatgatgg gacaaggatt tgcaattgat gcaaaaaacg aaacgattgt
     6541 atctccaatc gatggttttg tttcaagtat ttttcctaca aaacatgcct taggaataaa
     6601 aacaaaatca ggaattgaga tacttgttca tgtaggaata gatacagttg agctaaatgg
     6661 tgagggattt gatattaaag ttcaagaagg acaaaaagtt aaggctgggg atattcttat
     6721 gaccattaat tttgtagttt ttgaaaagaa tcacaagact aaagatgtca ttgttgtttt
     6781 tccagactac caagacgaaa ttttcattcc gaaacttgaa gaagtaaaac aaggacaaga
     6841 attgtttgtt cttggctaat gataagtaat taataataga atcgaggaaa atgtatggtt
     6901 tttggggcta ttgaagctgg tggaacaaaa tttgtttgtg ccattggaga taaagatttg
     6961 aacatatttg aaaaagtcag ctttcccacg actacaccca aagaaacaat gaatttagtt
     7021 ttagattttt ttaatcatca taaagatgat ttagaaggta tcggagtggg atcgttcggt
     7081 cctattgata ttcattgtga atctaaaacg tatggcttca ttacatcaac gccaaaactt
     7141 gcttggcagg attttgattt tattggaagt ttaaaaaagc aacttaatat tccaactgct
     7201 tggacgactg atgtaaatgc ctcttgctat ggtgaatatg tttctggaaa tggcagaggc
     7261 ctttcaagtg ttgtttacta taccgtagga acgggcatag gaggtggcgc tatccaaaac
     7321 ggaaacttca ttgaaggttt tagtcatcca gaaatgggac atatgcttgt aaaacgacac
     7381 aataaagaca acttttctgg gacatgtcca ttccacggag actgtttaga gggcttagct
     7441 gctggtccgg caatcgaaag aagatcaggg aaaaagggac aagaagtgac tgaaaatgac
     7501 gttacttggg aaatagaagc ttattacatt gctcagtgtg catataatac aacattgatg
     7561 ttatcacctg atgtcattat atttggtggc ggagtaatga aacaaaaaca cttaaaaaag
     7621 aaagtacaag ataagtttat tgagttgatc aacggatatg tcgctattcc cgatgttgac
     7681 aattatatta tgacaccaaa attgttagat aattctggta ttgtaggttg tttagcttta
     7741 gcaagagacg ttaaagaaaa atcttaattt ggaaaaaaga tcgtctcgac tatttgtttg
     7801 gttgatgcga tctttttttc aattagtaat tgaagggacg cctaaaagtg aaaaaaaaaa
     7861 tgaaagatga agtattagag tatatcaaaa ataatttaaa gtattacgat ttttctgctc
     7921 aagatattgc aatgaaattt tgtattaaaa gaaatgtcgc ttcccactat ttgaatcagt
     7981 tattttcaga tggaaaatta ctaaagaatg attctgttag accagtaatg ttcaagtata
     8041 atcaacaaaa gccaaaagac tgcttttcta agtttattgg agcagacata tctttgaaat
     8101 ccactataga taaatgcaaa gctactgtaa tgtatccccc taatggcctc ccattaataa
     8161 taaaaggaaa cagtggcgtt ggaaaaagtt ttttagcaag tttgatctat cagtatgctc
     8221 ttgatagaaa agtaattcat aatgacgcaa aatttgttgt tgtgaattgt gcagattatg
     8281 caaataatcc agagctattg tcagcagttt tatttggata taaaaaagga gcctttactg
     8341 gggctgaaaa agatactgca gggatgctat cttctgctaa tggaggatac ctttttttag
     8401 atgaggttca caatctaagt gcagaaaacc aagagaaatt gtttcttctt atggattctc
     8461 aaaagtatcg taaacttggt gaaagtgtgc aatgggagta tgcaaatgtt cgccttatct
     8521 tagccacaac agaagataaa aatacctctt tgttagcaac ttttagaaga cgcatcccag
     8581 ctgaaatcac tctgccagat tacaataata gaagtacatc tgaaaaaatt caattattat
     8641 ttgaatttct taaagatgaa gcaattacga ttgattcaaa aatattttgt tcgattgaac
     8701 tttttactga tcttttatcg aaaacatttg aaggaaatgt tggagaattg aggaatgaaa
     8761 tcaagcttct ttgtgcagaa ggttatttag gaaataagag aaagtcttca atttatttag
     8821 gggacaaact agacagcggt ttttggatag atccagaaat tagtatagat ttaaaaaaaa
     8881 tgtttcttaa atcactttct gaaattgatt tgattgaact ttttcaaaat cacatttcaa
     8941 ttgaagaaag catgagtcaa ttaagagata gaatacttaa agagtgtgca ggttcttctg
     9001 agtataatta tttagaaagt gacgaattta tagcattaag aaactatgtt gttaataaaa
     9061 tatcaccaat tattgattcg acaggtctat gtttattaga tgaattcttg gcggatatca
     9121 gtctatttat aatgtttatt gatgtaattg aagaaacatc aagaaatttt ttgctgtcta
     9181 agttcagaaa atttcgtatg aaaaatgata aacaaaaatt attggcaaat gagatttgga
     9241 gtagtttaga agtcgaagaa agtaaacaag aacttttgtt gaaatggata ctttttcttg
     9301 taaagaagtt ctatgtgaaa ataccagaaa cccattgtct gatagttatg cacggtaaaa
     9361 taacagcttc tgcgatcgca agggaaacaa ataagttgct gaatacatat gtttatgagg
     9421 ctttcgatat gccaattgaa ggtgagacta gtgacttaat taacctaatc aacaattttt
     9481 gtaaaagtat agatacgacg aatggactca ttctgctagt tgatatggga tcattggaac
     9541 aaatgtatga aaaaattgaa tccaatgtaa taggtgattt ggtaatttta aacaatgttt
     9601 ctacagcttt agcgattgaa tgtgggattc aagtttgcca aaaaaagcca attagtcatt
     9661 tttaccagat ggattttgat tcgtttcaag ttaaagttca gtattataaa ggattatcac
     9721 aaaagaaaaa tgtaatagta tcttgtctct ctggtgaggg gatttctgaa aaagttaagg
     9781 atattttaaa acggtatctc gataatcaag tggagatttt gatttttgat tttaacgcat
     9841 taaagaaaat agcaaaggaa aaagatacta tgatttttaa gaatacaatt tgtgttcttt
     9901 caaccactga attttacatt caaggaatcg attgtttaaa tttagaaaat ctaattaatg
     9961 ggaatcaaac cttagaaaaa ttaaataagt atatggactc ggatagctgt gagttctgct
    10021 taaatgaatt agttaaactc tttactattg aaggcgctag tactaagctt cgctttttag
    10081 attccaaaaa agtattcaat
//
LOCUS       DAJSBL010000078        10086 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.080, whole genome shotgun sequence.
ACCESSION   DAJSBL010000078 DAJSBL010000000
VERSION     DAJSBL010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10086
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.080"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(247..759)
                     /locus_tag="ONT85_002512"
     CDS             complement(247..759)
                     /locus_tag="ONT85_002512"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HCR2379456.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(773..3070)
                     /gene="recJ"
                     /locus_tag="ONT85_002513"
     CDS             complement(773..3070)
                     /gene="recJ"
                     /locus_tag="ONT85_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HCR2379457.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(3212..3655)
                     /locus_tag="ONT85_002514"
     CDS             complement(3212..3655)
                     /locus_tag="ONT85_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HCR2379458.1"
                     /translation="MKELKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPL
                     ILVIIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQRE
                     FRNERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(3656..4444)
                     /locus_tag="ONT85_002515"
     CDS             complement(3656..4444)
                     /locus_tag="ONT85_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR2379459.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(4441..5382)
                     /locus_tag="ONT85_002516"
     CDS             complement(4441..5382)
                     /locus_tag="ONT85_002516"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HCR2379460.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(5559..6146)
                     /locus_tag="ONT85_002517"
     CDS             complement(5559..6146)
                     /locus_tag="ONT85_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HCR2379461.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(6367..7674)
                     /gene="obgE"
                     /locus_tag="ONT85_002518"
     CDS             complement(6367..7674)
                     /gene="obgE"
                     /locus_tag="ONT85_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HCR2379462.1"
                     /translation="MFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGGDV
                     ILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAETGA
                     LLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVLAD
                     VGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGLIE
                     GASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLERP
                     QIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNATAD
                     LLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTNFD
                     HDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            7968..8978
                     /gene="gap"
                     /locus_tag="ONT85_002519"
     CDS             7968..8978
                     /gene="gap"
                     /locus_tag="ONT85_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HCR2379463.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(9063..9998)
                     /locus_tag="ONT85_002520"
     CDS             complement(9063..9998)
                     /locus_tag="ONT85_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR2379464.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
BASE COUNT         2778 a         2298 c         1746 g         3264 t
ORIGIN      
        1 ttttacgggg gtgtgacaaa aatcggtcac atcctttttt tcgaataaac ggtgttctaa
       61 agccagtttt tcggtattaa gtcaaattgc ggaaaaagtg attttttaaa cttctactgg
      121 tctttgcttg tttccattta taaaaaaata gcgaccctca aaagtcagat agagaatctg
      181 acttttgagg gtcgctttaa aatcgcttct ttaagaataa catcaagaat agcttctttt
      241 ttctgcttaa tattccatta atgtaatgat gtcatatcct ttgattttgt cgcgtccatg
      301 gagatccatc aattcgatca aaaatgcgca gcctacgact acaccgccta attgttcgat
      361 cagttcgatc gtcgctttga ttgttcctcc agtagctagc aaatcgtcac aaatcagcac
      421 acgttgacct ggtttaatgg catccttatg caatgtcaat gtatctgttc catattctaa
      481 tccataagtc acttcgatgg tttctcgagg taacttccct tttttgcgga ctggggcaaa
      541 accgactcct aactcataag caacaggaca tcctacgatg aatccacggg cttctggccc
      601 aactaccata tcaattcgtt tttcttttgc ataatttacg atttgtttag tagcttcccg
      661 ataagcgtca ccatctgcca tcagtgggga aatatctcga aacacaattc cttttgacgg
      721 atagtcggga atactggcaa tataatcttt taaatccact ctttgtttcc tcctaggttg
      781 ttaaccattt ctttatggta ggaatatcgc tcaaaagcaa aaattcctca actttgattt
      841 tattgagccg tcgctggtat aatctgctct cagtcaaagg atgactttct gggctagcga
      901 ctttttgtaa aacaccgttc tctattgtaa caaatttcaa ttcaaaaaac acctgtatca
      961 taaaaattaa taatttttca gggatacgca aatattgagc tattgctttt attttatgac
     1021 gtatatcaat tcgttcttga gaatggatca atttaaacaa ttttgcgtac tgttctcttg
     1081 tgcctacccc attcaaatag gcctcatcag gtgaaatccc catcagataa acacgattga
     1141 atgaataaga ccgaaaaact tctttcagat ccgctaaatc atctggacaa tccataacaa
     1201 ctagagagtt tggttttctt ttttcaatta ttgcctttac agaatctagt ccttcgaaaa
     1261 caatcgcagc ttcctggata atctcaggca cgaatttcaa cgtcgaagaa tcaaatgcta
     1321 aataaaggct accttccgac gttcggctct gttcacggaa acgttttgcc cgccaatcaa
     1381 atacttgcag accacccaca gaaaaatcag ataccatcag ctgcggtttc ttacgcccgt
     1441 tccattcatt gatcgaaagc tttcctacca catctacctg attattcagc agttcttctt
     1501 cttgagtacc aaaaccaaaa gcgaccgcat caagctgact cgatgcatcg gaaaggacaa
     1561 atttcagatg ctgttggttt gctccgattc ttttgatatt ttctgcttgg acttgtcgaa
     1621 ataaaaactg aggcaaagga ttatccgtac caaatggcgc caatagtttc agctcatcaa
     1681 ttcgctctac tgttacttca tttggcagca atacttcatc aattctcaac tcaggccctc
     1741 tcatcaagtc aatttgatga tcgactatat attggttcat cttctcctgt agtatagtga
     1801 cattttcact tggcatcgtt aaaccgactg ctgcatgatg tcctccgaag taagtaaata
     1861 aatctcgcat ctggtcaagc atttcaaaaa gattcaaggc ttcaatacta cgtccagatc
     1921 ctttagctga tccatcttct tttaaagtaa ggacaagcgt tggtttaccc gtttcattca
     1981 ttattttccc agcaacaatg cccaaaacgc cttcatgcca tcctcggttt accaatagat
     2041 ggatatgatt ctccttattg accatcgcta atgcttcttg tgttatctgc tctacgattg
     2101 cttttctttc ttcattgatc tcattcaatt tttttgcttg aacggatgct tcctcttcat
     2161 caaacgtcgc aagtaacgag acggcaggac gtggatcgcc catccgaccg attgcattta
     2221 gtctgggagc aatcgaaaaa ccgatcgttg tttcgtctgc gtccctcatt tttacgccgc
     2281 tttcttcaaa tagcgcctga agtcctatcc gttctgaatg atgaatggca tcgattccta
     2341 atgaaacaag gattcgattc tcatcagtca atgaaactaa atcggcgatc gttcctatcg
     2401 ctactaaatc caaaaattct gcaggcggtt cttccaataa tgcagaagct actttgaatg
     2461 ctactccgac tcctgccaaa tctggaaatg gatagtgccc cgctggatga cgagggtgaa
     2521 caatcgcata agcgtctggt aaattctcag gtagttcgtg atgatctgta atgatcacgt
     2581 ctacaccagc ttgctgagcg tatgctaccg catcatttcc tgctaccccg ttatcaacag
     2641 tgatgatcaa ctggattcct tcttcgatct tttcttggta taccgattgg tttggtccat
     2701 acccatgctc aaatcgattt ggcaggaata cttctacttc tgcaccaagt aattccagtg
     2761 tttctttcat taccgttgca ctggtgatcc catctgcgtc ataatcaccg taaacgagga
     2821 ttttttctcc actgattaca gcttcttgga ttcggctgat tgctttatcc atatcgtgta
     2881 tcagatacgg atcatgtaat tcttgttcct ttggatgtaa aaatcggttg atatcctttt
     2941 cctcatggta acctctttgc cacagtaact taccgacaaa tggagataat ttcagttcac
     3001 ggatcatttg gagaaaggat tcagaaggct ctgtctcgct taacttccaa tcaaaatttg
     3061 cttgtctcac tttctcactc ctaacttagt tattatagca aaggaaatca gcaaaaaaaa
     3121 gcagaaagaa agaataaaaa cagatcagat ccgctagaca gatgttcagc aggacctgat
     3181 cttttgtacg accatttatg aaatatccct attagtcaaa ataattgaag ctttgtttgg
     3241 gcgattgagt tttttgttct tccggcgctt tcaattgact gatttgtgcg gttttcgctt
     3301 gtaactccgc ctgataagca gcttccaact ccgcacgttc gtttctaaac tcgcgttgct
     3361 gttcttcttt tgcttcttca attttctttt gaagttctgt ttgatattct gacatttcct
     3421 gagtcagctg tttgatttgc tttttttgct gccatagtgt tgccgaagca gtcaaaaata
     3481 caatcagtgc accaatcagc gcagaaccga tgatcactaa gatcaatggc gcagaaaagg
     3541 aagtgaagcc aaaacttaca ggaacatttt tattgttcaa tacagcaaaa agaacaataa
     3601 tcaaaactaa aacaaatcca agaatcacat tgctttgctt tttcaattcc ttcacctact
     3661 ttttgttaaa aattccgctg gctaaataat caccaatcgt tggaaacagc gtataaaatt
     3721 ttgatgcaac ttctaaaata aacggttgat tgatttcccg ttttggatgt ctcatattgc
     3781 ggacgatcat ttttgccaac ttggcaggtt ctaaaacgat ttgtccgact ttttccaaat
     3841 agcttccgct tggatcagcc ttatcgaaga aatttgtctc gattggacct ggattcactg
     3901 tcgtaacagc tacgccaagt ggtttcaatt ctaatcgcaa agcgttggag aagccaagga
     3961 ctgcaaattt cgtggcagaa taaaccgttg attttgcagt tgccattttt ccagccatag
     4021 aggcgatatt aatgatatga ccttgtcttc tttctgccat atcgatcgca aatttttgtg
     4081 tcaaaaccat cattcctaag atgttgactt caaacatatc gtatgctttt ccaaggtcga
     4141 atgtaaggaa gtcttcgaaa atcccaaaac ccgcattgtt taccagaata tcgacgcgcc
     4201 cgacttcttc catcaccttt tcatataatt cgtccacgct ttcaggattg ctgacgtcta
     4261 ccttgaaagc ataagcctct ttgccgctca agcgtctgca ctcgtctctg acaccttcta
     4321 caaatgccat tcttcgtgcg caacttataa cgattgctcc ttgctttgct gcttcataac
     4381 agatttgtgc gcccagaccg cctgaactac ctgttactaa aacaacttta tcttctaaat
     4441 tcatgcctct tcctcctcgc gaaatggaat ctcaatgcta tcaaaatctt tcataatcgt
     4501 cgtgttttca aacacttctt gtgcttcttt ttcaagctct aaagctgctt ttcctaaata
     4561 tctcgcactg atatgagtaa gtagcagccg ttttgcctgc gcttcttttg caacttcagc
     4621 agcttgatga gtcgtggaat ggaaatagtt gtgcgccatt cttgcttcat ctttattaaa
     4681 tgtgctttca tgtaccagaa tatcgctatt ttccgcaagc acaacggaat tatgtgtttt
     4741 tctcgtatcg cctaaaatag tcacgattcg tcctttttta tctggaccta cgaaatcttt
     4801 tccattgatc gtccgcccat cttctagttg gatcgtttca ccttgcttca gtttgccgta
     4861 cagtggtcca gctggaatac ctaattcttt tagacgatcc acctgcaatt ctcctttatg
     4921 atcatgttca accacacgat aaccaaaact gtcgatccca tgatttagtt tttttgtata
     4981 aacagaaaat tgctgatcag taaaaatcgg ggctgtttca tttagctcaa tgaatttcag
     5041 cggataactt aaacgcgttt gcgaaatacc taaagagact tttatgtatt cctctatccc
     5101 ttttggcccg taaatctcta atggcgtatc cccaccttga aatgaccggc tgctgatgag
     5161 cccgggaagt ccaaaaatat gatcgccgtg taaatgtgta atgaaaattt taccgatttt
     5221 tctcggacga atagtcgttc gcaaaatctg catttgcgtg ccttcgccac agtcgaacaa
     5281 ccaaacttca ttgcgctcgt ctaaaagttt caatgctata cttgtcacat ttctatgttt
     5341 tgcaggaaca cctgcacctg ttcctaaaaa ttgtaattcc ataatctacc tgactttctt
     5401 taagttcctc tttaatttta gggtaaaaag aagggataat caaccgtaag tcgagaatgg
     5461 gttcaaatta aaaataacgt tctaaagcga tagcgttttc tttgaaaatt actataaaaa
     5521 cagcagcgaa cccgtttgag atcctctgct gttttttatt aatttgaaga tccagaactt
     5581 tcattgtcgt tggaagtttg cggatgtttg tccttccaag tcttttgtgc agcttctttc
     5641 gcatgatttt cagcttcttt cgcatcatgc acatcttttt cgacatcttc ataatccaag
     5701 cgagtaattt ctccgcctaa ttccatcatt accaattgat tcagtgggcg attgtcctct
     5761 tcatcagcaa tcaagatcag tcctgtagct ccttcaccga ttttatttcc tacaaagtcg
     5821 aacacggtct gggcatcttt tatttctttg gcatcttgtg tcgcaccgat cattcctccg
     5881 gcaaaccatc ctaataaaat ccctagcgga ccacctaata caccaaccaa cattccgatt
     5941 aatccacccg tagaagattt gttattaccg gtaaagtcga taaaatcttc tattttgaat
     6001 tgatgtgtcc cattaggatc atggctgaca actgccatct gttctccttt aatcgaacga
     6061 acagcatgta actttttgat ttctgaaaat gcctgatatg cctggctttc tacttgaaaa
     6121 tttaaaatga ttactcgttt gctcattaaa aacgcccctt tcataccttc ttgttattta
     6181 ttttaccttt aattgaaaaa taagaaaaga aaagcactct ttttttccaa taaaataggg
     6241 ctgtgacaaa agtcgtatca cagctccttt ttccgaataa acggcgttct tgaagctgat
     6301 cacttctgtc acaacctccc ttaaatctga atctatattt tttggtattt gtttttattt
     6361 gcttgcctat tcgacaaatt caaattcaaa ctcaccgata cgtacaaggt cgccatcttt
     6421 cgcaccgcgt gcccgaagag cttcatccac acccatacca cgtaattgac gagcaaaacg
     6481 catcactgtt tcatcatgat cgaaattcgt catttggaat aatttttcga tcttctcacc
     6541 agataagatc cacgttgcat ctgaatcacg atcgatttgg aactcaggac cttctggatt
     6601 gaatccatag tgtacagttt cttcttccag ttcttcttcg tacaatggga attctggcgt
     6661 tacttctaac aaatctgctg tagcattcaa taacgcgtcc aatccttgtc gagtcactcc
     6721 agaaattgga aatactggga tgtcatcagc aaattcatct tctttttcct tgttcagctg
     6781 ttctttgaat ttcactagat tttccggtgc atccggcata tccattttgt ttgccacgat
     6841 gatttgcgga cgctctagca agcgtaagtt ataagtagat aattctttgt tgatggcaag
     6901 atagtcttca taaggatctc gtccttccat cccactcata tcgatcacat gtaaaattac
     6961 tcgtgttctt tcgatatgac gaaggaattg tgtacccagc ccaacacctt gcgaagctcc
     7021 ttcaatcaag cctggcaagt ctgctgcggc aaaacttctg ccatcacttg tagtaaccat
     7081 tcctagatta ggtacgagcg tcgtgaaatg ataagcgccg atttttggac gagcagatga
     7141 aataacggat agtaaagtcg atttcccgac agatggaaaa cctacaagtc caacgtctgc
     7201 caatactttc aattctagtt cgatttttcg ctcttgtcct ggttcaccgt tttcagcaat
     7261 ttccggtgca ggattccgag gagaggcaaa acggatattc cctcgtccac cgcgtccacc
     7321 ttttgccaca actagtgttt gaccattttc aattaaatct cctaaaagag caccagtctc
     7381 cgcatctcgg acagtggttc cttgtggtac tttaacatag gtatcttctg aaccacgacc
     7441 gtgcatccct ttactcatcc cattttcacc tggctgcgct ttgaaatggc gattgaaccg
     7501 aaaatccatc aatgtacgta acccttcatc gacgatcaag atgacatcgc cgccacgacc
     7561 accgtctccg ccagccggtc cgccgtcagg aacgtatttt tcgcgacgaa atgcaaccat
     7621 tccgtctccg cccttgccag ctttcacatc gatcgttact tgatctaaaa acatggacat
     7681 aattaagtcc tcctattatt catttattta tttgactgcc tgaatttcca caataaacaa
     7741 aagtcttact tattttatcg gtgaaatctg attttgtcta tcatttcagc aaaaagattg
     7801 tttaccttct tcttttttta tcatttggta aggtttccaa ccttttttct gctaccaagt
     7861 ataatttttt gacaaaatat tctttgcgta gcagaataac agtgtattaa ttgtattaat
     7921 tgaggatgcc gtttcacagc acccctaata aaggaggaac ttttgatatg acagtaaaag
     7981 taggtattaa cggatttggg cgtattggcc gtctggcttt ccgccgcatc aaagaagtct
     8041 cagatgatat tgaagtagta gcaatcaacg acttaacaag tccaactatg ttagcacaat
     8101 tattgcaatt tgactctact catggtactt atcctggcac agttactgca acagaagact
     8161 caatcgtagt ggatggcgaa gcaactcgtg tttatgcaga acccgacgca agcaaaatcc
     8221 cttgggcaaa agaaaacggt gtagatatcg tactagaatg tactggtttt tatacatctg
     8281 aagaaaaagc aaaagcgcat ttagatgctg gtgtcaaacg agtagttatt tctgccccag
     8341 ctggtgctat gaaaaccatc gtctataacg tcaatgatga tacattagat gaaaacgaca
     8401 aaatcatctc agcaggttca tgtacaacaa actgtttagc acctatggct tatttcttaa
     8461 ataatgaatt tgggatcgaa gttggtacaa tgacaacagt tcatgcctac acttccacac
     8521 aaatgctctt agatggacct gtaaaaggcg gtaatttgcg tgctgctcgt tcagctgctg
     8581 acaatactat cccacattct actggtgctg cgaaagcaat cggcttagtt atccctgagc
     8641 tacaaggaaa attacaagga cacgcacaac gtgttccagt cgttgatggt tcattaactg
     8701 aattggtttc agtattgaaa acaaaagtaa ctgctgatca agtaaacgaa gcaatcaaaa
     8761 aacacacagt tgacaatcct tcattcgggt atgatgaccg tcaaatcgtt tctagcgatg
     8821 tcattggaac aactcaaggt tcgatctttg acccaacgca aacagaagta acaactgctg
     8881 gtgatttcca acttgttaaa actgttgcat ggtatgacaa tgagtatgga tttacttgcc
     8941 aaatgattcg cttactagaa aaattcgcta acttgtaatt tgatagatca atcgataaaa
     9001 gaagcctttg actggatcca gtcaaaggct tctttctttt catcccctac ttttaaactc
     9061 gttcaatgaa catattggct taaaaattgt tggactactt gctgatattt ttcaggatct
     9121 gtcttgtatg cttccgcatg ctcggctcct tttacaacat acttttcttt tggtcccttc
     9181 gttgcacgat agaccttatc taacatttca aatggtacaa atgtatcttc gtctccatgt
     9241 ataaagagca ttgggcgctg gtttttcttc aattgttcca ctgcatttgc ttcaccgaaa
     9301 aagtagccag cacggacttt tgtcactaga ctcgttactt gcataagcgg aaatgctgga
     9361 agactaaaca tatcgtctag ctgataagca agctcatcat gaacggaaga ataaccgcaa
     9421 tcttcaatga ttgctttcac attatctggt agtttttctc cgctagtcat cataactgtt
     9481 gctgctccca tgctgacacc gtatagaacg atttcttgcg attttccatt gttttctagc
     9541 actttgttta tccactgtac atagtcttta cgctctggcc atccaaatcc aatgtaatcc
     9601 ccttcgcttt ttccatgtcc tcttgcatca ggaacaagga cattgtaacc catatcatga
     9661 aacatctttg cgtaattggt catcgtttca gcgtttccca tatagccatg agccacaaga
     9721 gctgtttttt cacttttcgt ttcagcagga agataaatcg ctgataattt cagaccgtct
     9781 tttgaagtga tggtccaatc gcttcggttt tcttttgaac taaaccattt atgtgcatcc
     9841 gtttctttgg atgtctgtgt tgtaccaggt gtatcttcgt ctaaaaagtc tttctcagaa
     9901 ggcacaacag cataattata aaaataattc cctgcaaaca ctaacgcaac aatcagagct
     9961 aaagcgacgc ctaaaaggat tttcatccaa agtttcataa atcatccatc tcatttctct
    10021 tttttcatat aaccttgaat tagtataatc aaagaatctc tactatgcaa tgttcatttt
    10081 tataac
//
LOCUS       DAJSBL010000079        10023 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.081, whole genome shotgun sequence.
ACCESSION   DAJSBL010000079 DAJSBL010000000
VERSION     DAJSBL010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10023
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.081"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(487..711)
                     /locus_tag="ONT85_002521"
     CDS             complement(487..711)
                     /locus_tag="ONT85_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2379465.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            902..3679
                     /locus_tag="ONT85_002522"
     CDS             902..3679
                     /locus_tag="ONT85_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HCR2379466.1"
                     /translation="MNKLLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNT
                     VSHYLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQP
                     KDLFSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLH
                     DLLEDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLD
                     EVHRLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRI
                     PIRIELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGEL
                     KNIVKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLM
                     EKQQPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHEL
                     LVYWTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELE
                     QQVSQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIA
                     AHGYATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILV
                     DMGSLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPE
                     WRLLYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNS
                     DPLFSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQ
                     RIIRNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLI
                     ERLIRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSE
                     DTEFLSIEEEF"
     gene            3760..4179
                     /locus_tag="ONT85_002523"
     CDS             3760..4179
                     /locus_tag="ONT85_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HCR2379467.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            4191..4661
                     /locus_tag="ONT85_002524"
     CDS             4191..4661
                     /locus_tag="ONT85_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCR2379468.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            4678..5457
                     /locus_tag="ONT85_002525"
     CDS             4678..5457
                     /locus_tag="ONT85_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCR2379469.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            5444..6265
                     /locus_tag="ONT85_002526"
     CDS             5444..6265
                     /locus_tag="ONT85_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HCR2379470.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            6282..7331
                     /locus_tag="ONT85_002527"
     CDS             6282..7331
                     /locus_tag="ONT85_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HCR2379471.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            7344..8348
                     /locus_tag="ONT85_002528"
     CDS             7344..8348
                     /locus_tag="ONT85_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HCR2379472.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            8981..10000
                     /locus_tag="ONT85_002529"
     CDS             8981..10000
                     /locus_tag="ONT85_002529"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HCR2379473.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
BASE COUNT         3362 a         1651 c         2094 g         2916 t
ORIGIN      
        1 ttaatttgtt cttgcaatct tttttgctac gtaatttttg taaaaaagtg ggttgagaga
       61 aatctaattg attttcaaag aaggagtatg aaaatgagaa aaatatttat tcgtttattg
      121 tgggtactat cttagaggaa taaataatag tggagaaaac tatggtttgg cgcagattct
      181 atcaggtttg atggaacgaa gacaacctcc cgacatcaag tcaaaagcta cttgagattt
      241 tgatctgaaa ataaaaaaag aaaacaaggt aggaacatca atgtttctac cttgttttcg
      301 taatcaatgg gaaaaaacta agagtggtgt acgtaatcaa taaaaaggat ggtgacaaca
      361 gtacctcttt actctctgaa agcatcataa ctcgtttttc ctcattatgc caataaattg
      421 ttgttatacc acatataagg atttgtaata gctaaaaaca gacaggaaag ttgaaatctg
      481 tgaatcttat tttgtatgga aggatttata gaacgcaaaa atcaagataa agatgaataa
      541 aaatcctgtt tcggctatat attgcgtatc tacgccaata aaacgagttt gttctgtcat
      601 agccatataa agcaaaggca aaaatacaac tacagcgaga taagatccga tgatttctcg
      661 aaaagttttc caggaagtat gtttcctgtt tttatattta tccgatttca ttgaatagtc
      721 atagcctcct tattctttca tatgttgaaa ttccagtata aaaactggaa ggaactgtac
      781 gttttttaga ataaaaatta taagggataa gtaggctgct gtctataata agatatattt
      841 ttcaattctt tattaaagat tttattgaca aaatgaatcc gttttctatg atggaggagg
      901 ggtgaataag ttgttgaaga aggaaatttt atgttactta gagaaccaga cagccttttt
      961 cgatttagaa catatgaacg aggtctttac agcacaaagg ttagctgatc attttggggt
     1021 aaaaagaaac acagtcagtc attatttgaa ccagttaaat gatgagcaac tactagtcaa
     1081 aatcaattct cgaccagtga tttatttcca caaagaagct tttgaaaaac aattttttaa
     1141 gttacgcacg aatttttatt tttctatcga tgatctcaaa gaggaacagc cttttttcgc
     1201 gcaaccaaaa gacttattct cttttatgat cggatacgac gccagtttga aagaaagtat
     1261 cgaacaaatc aaaacagctc tttactatcc agatggcgga ttaccacttt tgattacagg
     1321 cgaaagtggc acaggtaaaa gttatttagt taatttggtc tatcagtatt gtctgcttca
     1381 tgatctgcta gaagattctg caccattcat cacttttaat tgtgcacaat atgcagataa
     1441 tcctgaactt ttgactagca atttgtttgg acatgttaag ggagcgtaca ctggtgcaga
     1501 agaaaataaa aaaggggctt ttgaagcagc agatggtggg atcttgtttt tagacgaggt
     1561 acatcgctta acaccagaag gtcaggaaaa actgttcacc tatcttgatc aaggtgtgat
     1621 ttatcgtatg ggagaaccta atttgtcacg ccggatcaaa acacgtctct tttttgctac
     1681 gactgaagag attactagtc atttcttaac aactttcatt cgaagaatac ctatccgaat
     1741 cgaactacct gcacttaatc agcgtagtcg taatgaacgg ctagaactga tttactcttt
     1801 ttttctcgaa gaacaaagaa aaatggggag ccctttgtca gtcagtggac cagctttgtc
     1861 acttttaaca agtcagaaat tatccggaaa cataggagag ctgaaaaata tcgtcaaagt
     1921 ttcagcagca aaagcatatg ctgaacagcg tgaacagcct gaaatccatg tcacgatcca
     1981 tcatttgcaa aaagaactac ttgcgcagcc tattactaaa aatacagcac agcaaggaat
     2041 ctatattacg caagaccaga ccttagagca attaatggag aagcaacagc ccgaacagca
     2101 gcgaatcgtt aaaagttttg agcaaatcct aattgatttc aagaaaaata actgtctgct
     2161 atattcttgc gaaggaaaat taaagcaaga agtgattcaa ttatttgatt ttttgctgtt
     2221 tgagaccagt agacaagaaa aacacgagct gctggtttat tggacacaat ctatccgaga
     2281 gacctttcga caaatggaaa ctgcctatca aatcaaattc gatggaaaca gtgtctatgc
     2341 cttgagttac tatctctatc agcgaagcac tatcaaatgg ttaccggaag aacatgatat
     2401 tatccaacta atcaaagaat tagaacaaca agtttctcag tcttatccat ctagttatca
     2461 ctatgtccaa cgaattctgg aattaagtaa gatcaaatta gatattgaag acacaagtat
     2521 ggatcggatt ttactgacga tctatctaaa aaaagcgaaa tggtcaaaag aaaaacgact
     2581 gccaaaagcg atgattgctg ctcacggtta tgcaactgct agcagtatgg caaatgtagt
     2641 aaatcgttta ttacaagagg atctatttga atcatttgat atgccactag atgtcacacc
     2701 acagcagatt gcttcagaaa tccttgatta ttgcgaaaac agcgatgttt caaatggatt
     2761 gatcatatta gtagacatgg gatctctgaa agaaatccat cagtttttta aaaaacagtt
     2821 atccgtaccg cttttgattt tgaataatgt gacgacacct ttagcaatca cagtagggga
     2881 atgcctgcaa aaaaatgtat cattagaaga gatagcagaa gagactgttc gacagatcca
     2941 gccagaatgg cgactgctct atccagagga aaacaaaccc aaagcactca ttactacttg
     3001 cttcactgga atcggtacgg cggtccactt aagtgaactg ctagaaaaaa gcttacctac
     3061 ggcttgtcag ttgaaaatca tcccatatga atatcagcaa ttaaaagata agaaaaacag
     3121 tgacccatta ttttccatat acgaagtcgt aggaatgatt ggcaccacag atcctgatat
     3181 caccaatatc gactatcttt cattagaaga cctcatctcg ggggaaaaga tgtctgtact
     3241 agctgaatgg ttggacacta ctatgaatgc ttcagagaag gaaatattca atcagcgcat
     3301 cattcgtaat ttttcattgg aaaaagtact ggattccgtg actattctag atacagagaa
     3361 ggtgatggga gaaatcgatc attttatgag agaattagag gtgcagttga atcgaagcat
     3421 cagcaatccc aaaaaattag cgttgtatgt ccatgtgagc tgtctcattg aacgattgat
     3481 ccgacaaatg ccaatcgaaa cctatcaagg attagagaaa tggaagcagt gtcaaaaaga
     3541 agggttatct gctattaaat ctgcgtttag tgtcattgaa gagcattata gtgtcatgat
     3601 ccccaattct gagattgcct atatctatga tattttgagt gaagatacag agtttctctc
     3661 gatagaagaa gaattttgaa cataagcaag ttgttgccac taattttggc atgcaacttg
     3721 cttttttagt atgtgtaagt gaaaagagga gatgaaaaag tggatagaga aattatctta
     3781 gcttcccatg gaaaatttgc ttcagggata ttagattcat tagagctgat ctatgggaaa
     3841 aatcattcta ttactgtatt cgattgctat actgatgaga attttgattt agcggaaaca
     3901 gtaaggcagc tttttatgag gtacaaagat aaagaaatca ttgttctgac ggatcttttt
     3961 ggcggaagtg tcaacaacga attcttacaa tatatcaatc gggatcatgt ctatctcgtt
     4021 gccggattga atttgccact attgattgag ctagtatctc ggttggacgc tgaagtagag
     4081 acaactgttt tgatccagca ggtgctaact gattcaaaaa aaatgatcca gttttgtaac
     4141 gagcggctca atcagaaaat agaagaagac gaattttagg aggaagaaaa atgattgtat
     4201 tgacaagagt cgaccatcgg ttattacacg gacaagtagc tttttcatgg acacaaacaa
     4261 taggcgcaga ctgtattttg attgctaatg atgatgtacc tacgaatgag atacgaaaga
     4321 caacgatcaa attagccaag ccacaaggag tcaaactagt gatcaaatca attgatgact
     4381 cgattgccgc gttgaaaagc ggggtgacag ataaatataa gttattcatc gtagtggaat
     4441 cgatcgagga tgcatataag ctggcagaag cgtatccaga aatcaaaacc atcaacttag
     4501 gagggatcaa ggcaaaacct ggtactcgca gcatcggaaa agcggtaaat attttaccag
     4561 aagaggagga acgcttgaaa cgattaattg acaaacaaat agaagtagaa atccgtcaag
     4621 taccagcaga taaaaaagtg gatgtaagag aagttttgta agaaaggaag aacctcaatg
     4681 attgtacaag cagtcttatt aggaattgtt gcatttgtcg cacaatcaga atatgcacta
     4741 gggacatcgt tgctttctcg tccgattgtg acggggctgt ttacaggaat cgtgttaggt
     4801 gatgtaaaaa ctgggatcat tatgggcgca actttagagt tagcatttat cggatcgttc
     4861 tcagtaggtg cttcgattcc acctgatgtt gtgaccggtg ggattttagg gacggctttt
     4921 gctattacag ctggagccgg tacagaaacc gcgcttttac taggtttgcc gattgctacg
     4981 ttgacattga tattgaaaaa tatttactta gggcttttga ttccaattat gaaccataaa
     5041 gcagacaatt atgcagaaga aggaaattat aaaggagtcg agcatatgca cttgcttgcc
     5101 ggtatcggtc tttccttgat gcttggtgtt gtggtaatgg tttcttatat ggtgggaagc
     5161 aatacgatcg gaaatctatt aaatatgatt cctgacttcg ttcaaaaggg gttgtcagta
     5221 gctacagggt tgattccagc tcttggtttt gcgatgcttg ctcgtttgct gatcaacaaa
     5281 caagttgcac catatttttt cttaggtttt gctatagctt cttacttaga gattcctgtg
     5341 accggagtag ctatttttgg tgcgatttta gcagttgtgg tagtcaatat tatgaatgtc
     5401 cgtcaactac cacttcaaac aactgcaggg gaggtagaag aagatgaaga attctgagat
     5461 caaaaacagc aaaataacga aaaaagagct aaatcacgta ttttggcgtt cttttcaaat
     5521 ggagttttct tggaattatg aacgtcagat gaatatggct tatgtttatg caatgattcc
     5581 gattttgaaa aagttatatc agtcgaaaga agaaatggca gcagctttga aacggcatct
     5641 agagtttttt aacacgaccc cacatatcgt tacactgatt ttaggaatca cggctgcaat
     5701 ggaggaagaa aataaggatg atcctgaatt tgatgtgact gcgatcgata gtatcaaaac
     5761 atctttgatg ggacctcttg cagggttagg agattctttc ttttggggaa ctttgcgatt
     5821 gattgccaca ggtgtgggta cctctttagc catgcaagga aatattttag gccctatctt
     5881 gttcattttg attttcaata ttccgcacat tctgtttcgc tatctagcaa caggttgggg
     5941 atatcgctta ggaacgggtt ttttgaaaaa gatccaggca aacggtatga tggaaagttt
     6001 gacattaggc gcgtctatta ttggactgat ggtcgttgga gcaatgactg ccacgatgat
     6061 caccatcaat attcctttaa aaatcggttc aggagaaaat gctgtgactg ttcaaagtat
     6121 ttttgatgat atcgttccaa atatcttatg tcttggagca tttggcatag tgttttatct
     6181 attgaagaaa gaagtgaaac cattaacgat cttgatcgga ctagctttct ttggaatttt
     6241 tggttcgtgg atagggattt tttaaaaaag gagcagagaa aatgtttacg atgcaagatt
     6301 atatctatga agaaaaggaa gtactaagta cgatcttgaa aaagaatgat ttttcgacaa
     6361 gagaacatat gaaaaaaaca gtgaatttac tgatcttagc aacgggttca tcttataacg
     6421 cttgtttagc agcgaagcca gcattggaat cttatggtga tcttactgtc gatattcaag
     6481 aaccttttta ttttttcaat tatggaaaac tttctccttc aatcgatact gtgattgctg
     6541 tgtcacaaag cgggaaaagt gcttctactg ttgaagcagt aaaaatgata caaaaacaag
     6601 ggttacctgt tgttgctatc acaaatgacg tgcaaagtcc acttgcttta gaagctgcac
     6661 aaattattga tttaggtgcc ggggtagaat cagtaggctt cgttacaaaa ggctattctg
     6721 cgactgtttt acagttacta ctcttaggga taggaattgg aataagtaaa aataaaatca
     6781 gcaaaaaaat agaacaagac tacatgcaac agttgcgcaa aataatcaac catttaccag
     6841 cgattattca aaaaacggaa gaattttttg atggatatca aagtttattt cgtttagcac
     6901 agcgtttcgt caccattgga tatggtccaa actggggaac agctaaagaa gcagagacga
     6961 aattgactga aacaatccgc gttccgtcac aaggatttga gttagaagct tatatgcacg
     7021 gcccatacct ggaggcagat gcttcgcatt tgctattctt tattgaaggg gaaagtgtaa
     7081 ataaggaacg ttcgcaaaag ttgcaaagat atatgagtcg gtacgttgga gagactctca
     7141 ccattaccac aaaaaaagca agaaacgaga aaacacttgg attagcaata gaatgtgatg
     7201 aatatctgtc tgtattagcg cttgtcgttc cgtttcaact attcgcttat aaaaccgcag
     7261 tagctaaagg tattgacttg aacaaaaaga tttttgaaga ctttgataca gtcttgaaaa
     7321 gtaaattata agaggtggaa atcatgctga aattcaatga agaagaacaa atcaaagcaa
     7381 taaaaggcgc actagcttta cgtccgcaag tagaagaaat catcgataaa ctttatgcgg
     7441 aaaaatttga cgctgtctat tatctaggaa tcggtggaac gtatgcttcc tcgatgcaag
     7501 ctgtcactta tatgaatggg aaaagcaact tgcctgtctt tgtacagcat gctgcagaat
     7561 attacactac tggaaacaaa agattaacaa aagattctat cgttattttg tcctctgtta
     7621 ccggtacaac tcaagaagta gtgaaagcag tagaacaaat caagaaagta ggagcgacac
     7681 tgattggatt tatcgataaa gcgaacagta agttgtctca gctatgcgat tttgtggtca
     7741 cttatccagc tcctggaaca gaacaaatca aattttttat ggcagcagat cgattgatgt
     7801 atttgaatgg agaattcgaa gcttattcag aatactatga acagctcgaa cgttatttac
     7861 caacagggtt agtggaagca gagaaaaaag cagatgcatt tggcttatca tttgcagaaa
     7921 aacatcgacg tgatagtatg cattatttta ttggagcagg gaatcaatgg ggagctgttt
     7981 attcatatgc catgtgttac tgggaagaac aaagctggct tccatcgaaa tctatccatg
     8041 ctgccgagtt tcttcacggg acgttagaga tcgtagaaga aacaacccca gtcacattat
     8101 ttttaggcga agatgaacag agagtgttat ctgaacgtgt ggctaaactt ttgccaaaaa
     8161 tttgcagcaa ttatacattg atagacacaa aagactatcc agtagaaggt atcagtgaaa
     8221 aatatcgcgg acgtgttttg tctttcttat tgatgcatgt agtgacacaa cgcatcgatg
     8281 ctcatgtgga gcagttgaac tgccatccat tagagatacg ccgctactat cgtcagtttg
     8341 attattagga tagactggat aaaaagaata taaaaaataa agagagcagc gataactgct
     8401 ccctttattt ttttaaaata ttggcatcaa ttacgttata aaacacattt tttgcattat
     8461 ctacagttca acttataatc tgtttaatgg gacatgtggg tgccaaccat tttttgttat
     8521 gcaatagtac tatttagttg ttgcatggat ccgtagtaaa agtccaagta caacgagttg
     8581 cgccagttta catagcgttt tgtatccaga atgttgacta aaacaaaagt tcctatgtca
     8641 accgtcaaca gagacaatct gtccgttgca ggacctgaca gatttagaaa tgttcaatct
     8701 gtctcttatg tattttaaat caatactcac taggagaagt ttcctaacct cctaatagta
     8761 atgctggtag taacattgtc aaaactgctt cctcttagga acaagcaaag cataacgagt
     8821 gtaaaaataa aatgtagact attgtaacat atcattgatt atttcaagta aaaacgataa
     8881 aagaagtaaa caaacactta aaaacaatgg aaattagtcc caactttaag aaatgtatac
     8941 tataatggaa attatcaatt atttatttgg aggaatatcc atgaaacaaa aaatcgcagt
     9001 tcttggtcct ggatcttggg gcactgcttt agcacaaaca ttggcagaaa acgggcatga
     9061 tgtccgtatt tggggaaatg tacctgaaca gattgatgaa atcaataagt atcatacaaa
     9121 tcagcatttt ttacctgatt tgacgatccc agaatcgatt atcggatata aagaattggc
     9181 agaagcagtg gataacgcag atgctatttt atttgttgtt ccaacaaaag cgattcgttc
     9241 agtcgcacag gaacttattg gtaaaatgaa tactaagcca atcattatac atgcaagtaa
     9301 aggactagaa caagatacgc ataaacgaat ctcagaagtt ttggctgaag aaattccaga
     9361 agaaaaaaga caggcaattg ttgtattatc tggtcctagc catgctgagg aagtggcagt
     9421 acatgatatc acaacaatta cagctgcaag tattgatcaa aaagcagcag cctatgtaca
     9481 aaaactgttc atgaatgatt atttcaggat ttatacaaat aatgatgtga ttggcgtaga
     9541 aactggtgca gcactgaaaa atatcattgc aattggtgcc ggcgctatcc atggattagg
     9601 attcggagat gatgcaaaag cagcaatcat gactcgagga ttggcagaaa tcagccgtct
     9661 tggtgtcgcg atgggtgcca atcctctgac atttatcggt ttgagtggtg tgggggactt
     9721 gatcgttact tgtacaagtg ttcattctcg caattggcgt gccggtaact tgttaggaca
     9781 gggacataag ctggaagaag ttctagaaaa catgggaatg gtcgttgagg gtgtggcaac
     9841 gacaaaagca gcagtagagc tagctcaaca gctaggtgtt gagatgccaa ttactcaaac
     9901 aatctataat gttttgtata atggagaaga tatcaaaaaa gcagcaaaag aaatcatgtt
     9961 acgtgacgga aaaatggaga atgaattttc tcttcgttaa atagatgatc aatagttaat
    10021 cgt
//
LOCUS       DAJSBL010000080         9638 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.082, whole genome shotgun sequence.
ACCESSION   DAJSBL010000080 DAJSBL010000000
VERSION     DAJSBL010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9638
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.082"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(252..674)
                     /locus_tag="ONT85_002530"
     CDS             complement(252..674)
                     /locus_tag="ONT85_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HCR2379474.1"
                     /translation="MRNKKVIVRILIVSFLLFGCFKLIKTNEESNGKLINSNLEEVLE
                     LQKTNQSFFVYLARNDCKKCQEVESFLERYDKKFSRNVYRIETRKEPRQEELQTFLQR
                     YHVKSVPSFLVIHDRNVKKIERKTFFFEKTLNQFLPNF"
     gene            complement(664..2838)
                     /locus_tag="ONT85_002531"
     CDS             complement(664..2838)
                     /locus_tag="ONT85_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 family protein"
                     /protein_id="HCR2379475.1"
                     /translation="MMNAPKRPTHFEQMFSVDRKRKQEKETPKSRFQQLYFIVTLLGV
                     CLYPFFIVGSIPLAIVFMIDKKETRAHIYDFEYESFLKRRSSLFFIFALGFTLLNLFC
                     FIFVLPRGYLSCYLLFPLNLFSHALQFNGQTIFALLLGGTGAGSCLIAYASFIEKRKV
                     VSKKERQTALLNSKEYKERRKNKFQESQKYTAEYQQAYQVAIVIKDVALRNKQLASLA
                     KVILLGTDEYGLPYVMNFSELNQHVIIPATTGSGKTTVIQLLAEHACKFRIPVILVDG
                     KGAGETLQALKEVAEAYNRPIEVFVDGGDLRYNPVKNGNDIAIRDKLTNLAETESVYY
                     STAAKALLQVTIQLLDEFQKSHKIERNLVWLQHYLLPRNVLHLFADRIQLKNPDLFKE
                     KVVVEKKKTTKKKTKKNEAMDKTERDFADVEQLSEERIVEHDRKTVDLETYYLLLKEN
                     ISLMTEIEQTLFKRLFVRYEHKKNPFYLYATSENLQTNINMLLDSELGELFDTSGGKR
                     ELDIQEVVKEGKIVYISLNGLIYSEFIRTLAQMLVGDINFFASEVYKQAGETANRSKV
                     LVIFDEPASYLNDNLIDLVNKGRGAGVHAAYAPQTLADIDRIDPVLRKQLIGNVNTFF
                     IGKTNDPTEIDYWADLMGTEEDIELTEMTTQEAGYSDMGKVDWTGERGTKRDVDRFKF
                     NPNRIRALRTGEFVVYRTAEDVQEPPRVVYIRKPNLEKQYAK"
     gene            complement(2854..5196)
                     /locus_tag="ONT85_002532"
     CDS             complement(2854..5196)
                     /locus_tag="ONT85_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2379476.1"
                     /translation="MRKKSLKWWTLLLLGGLFFCLQGATVSADQMNDGAGVTETDRSE
                     GENANDKVEVKEVTTDVDQLYKNYKKNSFELMTNDYESMNLKKKLSYNVAGVMKNFVW
                     SGVKMLGEFNASMVEFLFELDIISAIREPIQNLTQKIADNMLSIAGTIGISFSAIMMA
                     IKYAGEARMKQALRVFLMTILIFTGLSVLKDSGTNKNLFDVLFSLDSTVETAFVKVNP
                     VLTPEEETNYETKETEEVTADKKVETAGKLMAARVFYSNVYEPYLLLNYGTTNVDTIR
                     EKKVKYAGKEYDRINLLLDNDLGTKIGEKIHDKVTEYEADTLKNKNIQYYNSWKNTAF
                     ALFYFVVNLIQTVVYFVLSMFRLIIGTMQLLLVPMLPFLLFLGLFLVTMNVFGNYAKV
                     FGILTFLKGMTGFACILFSSFLSYGFQLSSTQDDPWEKILLIIVYLFAPLGIYLFRGF
                     LGSLFTGRMGVRGALAFAGNPFGAEAKLRREAKQRLKERKEARKAQKQKEKENARKRQ
                     ERQRKRGGGSDVRMPQTAKQATRSAFRRESAQQPRHKAPNRMERMAQKARDLHDQAKQ
                     AEEQSQERMQRTAMEKQARKTADLLQRELQGNPSLRERSRKGASRSPYRNQGSAMVVS
                     LANPKQKHKSYGQKHTPRTELNDRKRRQGQTSRRRKEALTPVTKTSSMKTRTVAGQKH
                     AVQKPLRKGNVPRATGSTTPKSVRRPEGVREHMSAVNRVTAQTKAPSTVSKAYSTMKQ
                     TKSPKQAPKTRSEFRATQAKMKRKREKQPIYRREMKQRKR"
     gene            complement(5218..7761)
                     /locus_tag="ONT85_002533"
     CDS             complement(5218..7761)
                     /locus_tag="ONT85_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCR2379477.1"
                     /translation="MAFLFLLVVFIIGIWQFKKKKTKLPRLEFPIDAIIRNFVLTKND
                     DVWVGYQLSSQVFPLNDIDFFKNYLQDGEGILGHDEYDYHFINIPRQFNLDTHIEETI
                     RDLVKGECADLGKVYFEQAGKILKDEVQLNEYRTYLFVRFTTEMKVSDPIEYFQLLKH
                     VFKKFIYRITGQRVPISSVLPTYETLEKELYTELSNYKPIERLSAQEIGQIYYYFFHR
                     SEEKLPERQLTPMEICEGVISNEAGYIAIEQLNQTHYSTFLSQTDAPLAMYGSSFIQN
                     LQDSLSFPIDTHIRLRFEHSKSDERKINKMAGRIYEQEKEYETVDGILDSDDVLNFGT
                     ERLMDVKDKLKRKEKRLCYSTVTLVVSASSKEELEERVKDIEFAIESTDFKFYRSVAD
                     QLTLFNQCLIGSTQRFRSYEQVATTGFVADYGLDLKKEVGNKKGFPLGRIVIAKKFKS
                     AKEALALSSKLVWYYPNLTKKSIEGAEHTNGNTLIIGPPGKGKSVLVKYIFLWLMFLG
                     QKILYIDPKNETQKFFLKALEKFGHIPEFVAMYERINFISLSSDESCRGMLDPLLFLP
                     KEEAIQTAKNVLGMLGEVDTNRETASHKKTVIQDAIDVVYTMPGKHSLSQVIEEIRKS
                     DKELAKLILGHSVGLGKILIGNEHSTPIRFDSQINVLGTQGIRIPTQKEIDSGRLNSE
                     QLAGMAIMEVIMKFTYIFSTNKEEDAAIIYDEAKGYEDTAQGSYLIDDSLRKGRANNT
                     DIYLVVQAFKDYDQEDKKELISYKFAFRPKQKEAREKLLSFFDMEVNSANLEMMEQLQ
                     TGTCLFQDHLGRNQPIAIDVLFDSWLLAVSSTENQDEKIQAMLENEKQR"
     gene            complement(7771..8169)
                     /locus_tag="ONT85_002534"
     CDS             complement(7771..8169)
                     /locus_tag="ONT85_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HCR2379478.1"
                     /translation="MEYNYTKEFKHPHKIYGIKGQKIPWISNGIRMEQLVIGGVFLFI
                     LLILFLIAYVNQISFLQTLFRKSWLIMIFGIGVLVWTLFSMKWDNKHFFEYLIGRTSY
                     YQKRKQRFEHEMYVPFFHEKVTYSLHKWKE"
     gene            complement(8250..8534)
                     /locus_tag="ONT85_002535"
     CDS             complement(8250..8534)
                     /locus_tag="ONT85_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2379479.1"
                     /translation="MKLFDLKSLFDDVSGQVIWLLAIVAIVLMVGAYATQGFARAAFA
                     IIGVFILIMLVLLLSNAVDIGEWLKNIIFIKDSEENVGVTQMIVPWIQLS"
     gene            complement(8558..9556)
                     /locus_tag="ONT85_002536"
     CDS             complement(8558..9556)
                     /locus_tag="ONT85_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HCR2379480.1"
                     /translation="MSNAKFNPQAFQTKPPKQQKEKKQKKRFLFQKKKTEPSDRMIQF
                     SQKSAMSVFSGAFFCLFLLMGVVLLLNFGRVDTLTRLAVKKQVNPETLLTEVNQTLSS
                     TEQLKYEGQHFIERLFTYSPEKRNEWEASVNHYLAQGLEASDLGFVTTATNRVAKDVR
                     FIKLEEIDLTQAMYRLFYEVTFTEGEHWKSSQLSFPVSYQNNVLKVLDGVQFLNLSES
                     KNTNSVNYQENRFYSSGQAVTESEEIKLEAFVKRFFELYVKNDEKLALISNEKGLEGG
                     ELINVQLDHAVKNKEGNYFLQGKYRFYFEKGNEFQSNFSMEVKTSGDSYFVTTLNE"
BASE COUNT         2769 a         2038 c         1495 g         3336 t
ORIGIN      
        1 atactgaaac gtaaaaaaaa taaacggatt tcaaacaaaa tacgtttatt ttttctattg
       61 attttagctc aaaatcgatt caattcgagt gaaatttgct tcaattttca attggttttt
      121 gttcaaattt tgaaacattt ttgctcaaaa aatgagtgaa tttgtttcaa aagaaaaaca
      181 aatacgatcg aaaaaaattt gaactcataa caattttttg tttttttcaa cgtattctct
      241 ttcaattttc actaaaaatt aggaagaaat tgattcaatg tcttttcaaa aaagaatgtt
      301 tttctctcaa tttttttgac gtttctatca tgaatcacga gaaaacttgg gacactttta
      361 acatggtaac gttgtaaaaa agtttgaagt tcttcttgtc ttggttcttt tcttgtttca
      421 attcgatata cattcctaga gaattttttg tcgtatcttt ccaagaaaga ttcaacttct
      481 tggcattttt tacaatcatt tcttgctaga tacacaaaaa atgattgatt cgttttttga
      541 agttctagta cttcttctaa attactattg attagttttc catttgattc ttcgttagtt
      601 tttatcagtt taaaacaacc aaataagagg aaactaacaa taagtatcct aacaataact
      661 ttcttatttc gcatattgtt tctccaaatt tggtttccga atatagacga ctcgcggtgg
      721 ttcctggacg tcttcagctg ttcgatacac cacaaattcg cctgttctca atgctcgaat
      781 gcgattcgga ttgaatttaa agcggtctac gtctcgtttc gtgcctcttt ctcctgtcca
      841 atcaaccttt cccatatcag aataacctgc ttcttgtgtc gtcatttccg ttaattcaat
      901 gtcttcttct gtccccatta aatctgccca ataatcaatt tctgtcgggt catttgtctt
      961 cccaataaag aacgtattca cattcccaat caactgtttt cgaaggacag gatcaattct
     1021 atcaatgtcc gcaagcgttt gaggggcata cgccgcatgc accccagcgc ctcgtccctt
     1081 attgactaag tctataaggt tatcatttag ataagaagct ggttcgtcaa atattactag
     1141 gactttcgag cgattggctg tttctcctgc ttgtttatac acttctgacg caaagaaatt
     1201 aatatctccc acaagcattt gagccaatgt ccgaataaac tcactataaa tcaatccgtt
     1261 taacgagata taaacaatct ttccttcttt aacaacttct tgaatatcta attctctttt
     1321 tcctccagaa gtatcaaaga gttcaccaag ctcactatcc aataacatat tgatattggt
     1381 ctgtaaattc tcagaagtcg catacaaata aaacgggttc ttcttatgtt cgtaacgaac
     1441 aaacaaccgt ttaaacaacg tttgctcgat ttctgtcatg agagaaatgt tttcttttaa
     1501 taatagataa tacgtttcta agtcaacggt tttacgatca tgttcaacga ttcgctcttc
     1561 acttaactgt tccacatcgg caaaatcacg ttctgttttg tccatcgctt cattcttttt
     1621 cgtttttttc ttcgtggttt tcttcttttc tacgaccact ttttctttaa ataaatctgg
     1681 atttttcaac tgaatacgat ccgcaaacaa atgtaagacg tttcgtggca ataaataatg
     1741 ttgtaaccaa actagatttc tttcaatttt atggctcttt tggaactcgt ctaaaagctg
     1801 aatcgttacc tgtaataacg ctttggctgc tgtcgaataa tacacgcttt ctgtttctgc
     1861 tagattggtt aatttatctc gaatggcaat atcattgcca ttttttacag gattataacg
     1921 caaatcgcca ccgtccacaa agacttcaat cgggcgattg tatgcctctg ccacttcttt
     1981 taacgcttgt aaagtctctc ctgctccttt cccatcgact aagataacgg ggatacgaaa
     2041 cttacacgca tgttctgcta gtaattggat caccgtcgtt tttcctgaac ctgtagtcgc
     2101 tggaataatc acatgttggt tcaattcact aaagttcatc acataaggga gcccatactc
     2161 atctgttcct aatagaatca ctttggctaa tgaagccagt tgtttattcc gcaaagcaac
     2221 gtcttttatg acaattgcta cctggtacgc ttgctgatac tctgctgtat atttttggga
     2281 ctcctgaaat ttattcttcc gacgttcctt gtattcttta ctatttagca aagctgtttg
     2341 ccgttctttc tttgaaacaa cttttctttt ttcaataaaa gacgcatacg caatcaaaca
     2401 acttcctgca cctgtcccac ctaaaaggag ggcaaaaatc gtttgtccat taaactgcaa
     2461 cgcatgagaa aacaagttta atggaaataa taagtaacac gacaaatacc ctctaggaag
     2521 aacaaaaata aaacaaaata gatttaatag cgtaaaacct aacgcaaaaa taaaaaaaag
     2581 agagcttctt cgttttaaga agctctcata ctcaaagtca taaatatgtg cacgtgtctc
     2641 ttttttatct atcataaaga caatcgctaa aggaatactg cctacaataa aaaatggata
     2701 tagacaaaca cctaataaag tgacgataaa ataaagttgt tgaaaccgac tttttggcgt
     2761 ttctttttct tgttttcttt tgcgatcgac agaaaacatt tgctcaaaat gtgtcggtcg
     2821 ttttggtgca ttcatcatac tatcgatttc ctcctatcgt tttcgttgtt tcatttctct
     2881 tcgataaatc ggttgttttt ctcgttttcg tttcattttt gcttgagttg ctcgaaactc
     2941 actccgtgtc ttcggtgctt gttttggtga ttttgtttgt ttcatggttg agtatgcttt
     3001 agatacggtt gaaggcgctt tggtttgtgc tgttactcga ttgactgctg acatatgttc
     3061 ccgaaccccc tctggacgtc gaacagattt cggtgtggta gaacctgttg ctcgtggtac
     3121 attccctttt cttagtggtt tttgtaccgc atgtttttgt cctgcaactg tccgggtttt
     3181 catcgaactt gtttttgtta cgggagtaag tgcctccttc cgacgacgac ttgtttgtcc
     3241 ctgacgacgt tttcgatcgt tcaattctgt tctaggcgta tgcttctggc cataagactt
     3301 gtgtttttgc tttggattcg ctaatgacac caccattgca gaaccctgat tgcgatacgg
     3361 actacgagac gcaccttttc tacttctttc tcttagacta ggattgcctt gcaattctcg
     3421 ttgtaataaa tcggctgttt ttcttgcctg tttctccatt gctgttcgtt gcatacgttc
     3481 ttgactttgc tcttctgctt gtttcgcttg atcatgcaag tctcttgctt tttgtgccat
     3541 acgttccata cgatttggtg ctttatgacg aggttgttgt gcggattcac gccgaaaagc
     3601 acttcgtgtg gcttgtttcg ccgtttgtgg catacgaacg tctgagccac ccccacgttt
     3661 tcgttgacgt tcttgtctct tccgtgcgtt ttctttttct ttttgtttct gtgcttttct
     3721 cgcttctttg cgttctttca accgttgttt ggcttccctt ctcaattttg cttctgcccc
     3781 aaatggattt cccgcaaaag ctaacgctcc tcgaacaccc attcttccag taaatagact
     3841 gcctaaaaag cctcggaaca aataaattcc caacggcgca aacaaataaa caatgatcaa
     3901 gagaattttt tcccatggat cgtcttgtgt actacttaat tggaacccgt aactaagaaa
     3961 agaagaaaaa aggatacatg caaaacctgt cattcctttt aaaaacgtca aaataccaaa
     4021 aactttggca tagttaccaa acacattcat tgtgacaaga aacaatccta aaaaaagtaa
     4081 gaacggcaac atcggtacta acaatagttg catagttcca ataatcaatc gaaacatact
     4141 taagacaaaa taaacaacgg tttgaatcag attcacgaca aaataaaaca aggcaaatgc
     4201 tgtatttttc caactattgt aatattgtat gttcttgttt ttcagtgtat ctgcttcata
     4261 ctctgtgact ttgtcatgaa ttttctcccc tatttttgta cctaaatcat tgtccaataa
     4321 aagattgatt cgatcatatt cttttccagc atatttgact ttcttctcac gaattgtgtc
     4381 tacattggtt gtgccataat ttaacaacag atatggttca taaacattac tataaaagac
     4441 ccgagccgcc atcaatttgc ctgccgtttc cactttttta tccgctgtta cctcttctgt
     4501 ttcttttgtt tcataatttg tttcttcttc aggtgttaac actggattga ccttcacgaa
     4561 cgccgtttcc actgtagaat ctaaagaaaa aagaacatca aaaagattct tatttgtccc
     4621 agaatctttt aatacgctta acccagtaaa aataaggatc gtcataagaa agacacggag
     4681 tgcttgtttc attcgagcct ctccagcata tttgattgcc atcataattg ccgaaaaact
     4741 gattccaata gttcctgcaa tacttaacat attgtccgca attttttgtg ttaagttttg
     4801 aatgggttct cgaatggcgg aaataatatc taattcaaag agaaattcaa ccattgaagc
     4861 attgaattct cctaacattt ttacgccact ccaaacaaaa tttttcatca ctcctgcaac
     4921 attgtacgat agcttttttt taaggttcat tgactcatag tcattggtca tcaattcaaa
     4981 cgaattcttt ttgtaatttt tatacaattg atccacatca gtcgttactt cttttacttc
     5041 tactttatcg ttggcattct ctccttcaga acgatcggtt tcagtgacac ccgcaccatc
     5101 attcatttga tcggcagaaa cagtagcccc ttgtagacag aaaaaaagac cccctaataa
     5161 aaggagcgtc caccatttca gagatttttt tcgcaaaaaa accacctctt ttttgtttta
     5221 tcgttgtttt tcattttcta acattgcttg aattttttca tcttgatttt cagtagaaga
     5281 aacagccaat aaccaactat caaacaacac atcaatggca atcggttgat ttcttcccaa
     5341 atgatcctgg aataaacagg ttcctgtttg taattgttcc atcatttcta aattggctga
     5401 attcacttcc atatcaaaga aggacaatag tttctctcgt gcttcttttt gtttcggacg
     5461 aaacgcaaat ttgtaagaaa ttaactcttt tttatcttct tgatcgtaat ctttaaacgc
     5521 ttgcacaaca aggtaaatat ccgtattatt cgcacgtcct tttcgcaagc tatcatcaat
     5581 caaatagctt ccttgtgcgg tatcttcata cccttttgcc tcgtcataaa taatcgcagc
     5641 gtcttcttct ttgtttgtag aaaagatata cgtaaacttc ataatgactt ccataattgc
     5701 cattcctgca agttgttcgc tatttaatcg acccgaatca atctcttttt gtgtcggaat
     5761 gcgtattcct tgtgtcccta acacgttgat ttgtgaatca aaacgtatcg gcgtactgtg
     5821 ctcattccca atgagaatct ttcctaaacc aacgctatgc cctaaaatca atttagctag
     5881 ttctttgtca ctttttcgaa tttcttcgat aacttgactt aacgaatgct tacctggcat
     5941 ggtgtaaact acatcgatgg cgtcttggat caccgttttc ttgtggctcg ctgtttctcg
     6001 attggtatct acttctccta acattcctaa cacattttta gcggtttgaa ttgcctcttc
     6061 ttttggtaaa aacaatagag gatcaagcat ccctcgacag ctttcatctg aagataacga
     6121 aataaaatta attcgttcat acatagccac aaactctggt atgtgtccga atttttcaag
     6181 tgcctttaaa aagaactttt gggtttcatt tttcggatca atataaagaa ttttttgacc
     6241 taaaaacatc agccataaaa aaatatattt tacaaggacc gattttcctt tacctggtgg
     6301 accaataatc aacgtatttc cattggtatg ttccgcgcct tcaatacttt tctttgttaa
     6361 attgggataa taccatacta gtttactaga tagcgctaac gcttctttcg cagatttaaa
     6421 cttttttgca ataacaatcc gtcctaaagg aaatcctttc ttatttccta cttctttctt
     6481 taagtccaag ccataatctg ccacaaaacc tgtcgtagcg acctgctcgt aactacgaaa
     6541 acgttgcgta cttccaatta agcattgatt aaacaacgtt agctgatccg ctaccgatcg
     6601 ataaaatttg aaatctgtag actcaatcgc aaattcaata tctttgactc gttcttccaa
     6661 ttcttctttt gaagaagcag atacaaccag cgtgacggta gaataacaca atcgtttttc
     6721 tttgcgcttc agcttatctt ttacatccat taaccgttct gtaccaaaat ttaatacatc
     6781 gtctgaatct aaaatgccgt caacggtctc atattctttt tcttgttcat aaatccgtcc
     6841 agccatttta ttaatttttc gttcatcaga ttttgaatgc tcaaatcgaa gtcgaatatg
     6901 cgtatcaatt ggaaaagata agctatcctg aagattttga ataaagctac tcccatacat
     6961 ggctaaagga gcgtccgttt gacttaaaaa cgtcgaataa tgcgtttgat tcaactgttc
     7021 aatcgcaatg tatcctgctt cattcgaaat gacgccctcg caaatttcca ttggcgtaag
     7081 ctgtctttca ggcaattttt cttctgatcg atggaaaaaa taataataaa tttgtccaat
     7141 ctcttgggca gataagcgct ctatcggctt ataattggac aattccgtat agagctcctt
     7201 ttctaatgtt tcatacgttg gtaacacact acttatagga actcgttgtc ccgtaatacg
     7261 atagataaac tttttaaaaa catgcttaag caattgaaaa tattcgatcg gatcagacac
     7321 tttcatttct gtagtgaaac gcacaaacag atacgttcga tactcgttta attgcacttc
     7381 gtcttttaaa atctttcctg cctgttcaaa gtacactttc ccaagatctg cgcattctcc
     7441 ctttaccaaa tctcgaatgg tttcttcaat gtgcgtatct aggttaaatt gacgtggaat
     7501 attgataaaa tgatagtcat attcatcatg tccaagtatg ccttcgccat cttgaagata
     7561 gttcttgaaa aaatcaatgt catttaaagg aaatacttgg gaagaaagtt gatagcctac
     7621 ccacacatca tcatttttgg tcaaaacaaa atttcggata atggcgtcta tcggaaattc
     7681 taatcgagga agcttcgttt ttttcttttt gaactgccaa atcccgataa taaagacgac
     7741 caaaagaaac aaaaacgcca tttactcgac tcactccttc catttgtgta gggaatacgt
     7801 cactttttca tggaaaaacg gcacatacat ttcatgttca aagcgttgtt tccgtttctg
     7861 ataataggac gttcgcccaa ttaaatactc gaaaaagtgt ttattgtccc atttcattga
     7921 aaacaatgtc caaacgagaa caccaatccc aaaaatcata atcaaccagg actttcgaaa
     7981 cagtgtctgc aagaaactaa tttgattcac atacgcaatc aaaaatagga tcaaaagaat
     8041 gaagagaaat accccaccta tgactaattg ttccatgcga atgccattag aaatccaggg
     8101 aattttttgt cctttaatcc cgtaaatttt gtgagggtgc ttaaactcct ttgtatagtt
     8161 gtattccatg ttcactctac ctccttttac aaaaaaagaa gcgaccagaa gttctaatct
     8221 tctgatcgct tttgcagttt gtctttttct tatgataatt gtatccaggg cacaatcatt
     8281 tgcgttaccc ccacgttctc ttcactatcc ttaataaaga taatgttctt tagccattca
     8341 ccaatatcaa cggcattcga taacaataag acgagcatta tcaaaataaa gacgccaata
     8401 atggcaaacg ctgcccgcgc aaagccttgg gtcgcataag caccaaccat tagaacaatc
     8461 gcaacgattg ccaatagcca aatgacttgt ccagatacat catcaaataa cgatttaaga
     8521 tcaaaaagtt tcatttccat tctcctctaa gttcatttta ttcatttaac gtagtaacaa
     8581 aataactatc gccacttgtt tttacttcca ttgaaaagtt tgattggaac tcgtttcctt
     8641 tctcaaaata gaaacgatac ttcccttgca aaaagtagtt tccttcttta ttttttactg
     8701 catgatccag ttgtacattg atcaattcgc cgccttctaa ccctttttcg ttagaaatca
     8761 atgccaattt ttcatcattt ttgacatata actcaaaaaa ccgtttcaca aatgcttcca
     8821 gcttgatttc ttcgctttca gtgaccgctt gcccagaaga ataaaatcga ttttcctgat
     8881 agttcacaga attggtattc ttactctcag aaagattcaa aaattgaacg ccatcaagta
     8941 cttttaaaac attgttttga taactgactg gaaaagaaag ttgactgctt ttccaatgtt
     9001 ctccttctgt aaaagtaacc tcataaaata ggcgatacat tgcttgtgtc aaatcgattt
     9061 cttctagttt aataaagcga acatctttcg ctactcgatt ggtagccgtc gttacaaagc
     9121 ctagatcaga cgcttccaat ccttgtgcta aataatgatt gacggaagcc tcccattcgt
     9181 ttcgtttttc tggcgaataa gtaaataacc tttcaataaa atgttgtccc tcgtacttca
     9241 attgttccgt agaagacaag gtttggttga cttcagttaa caatgtttct ggattgactt
     9301 gttttttcac tgctaaacgc gttaatgtat ccactcgtcc aaagtttagg aggagcacga
     9361 cacccattaa cagaaacaaa caaaaaaaag cgcctgaaaa tacactcatg gcactttttt
     9421 gcgaaaattg gatcattcga tctgacggtt ctgttttctt tttttggaac aaaaaacgtt
     9481 tcttttgttt cttttccttc tgttgtttcg gtggcttagt ttggaacgct tgtggattaa
     9541 attttgcatt actcatgaga aatgccatac cgttctaaca ataatttcgg tattttgtcc
     9601 acaatcacta cttcgtggac aaccacactt gccccctc
//
LOCUS       DAJSBL010000081         9444 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.083, whole genome shotgun sequence.
ACCESSION   DAJSBL010000081 DAJSBL010000000
VERSION     DAJSBL010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9444
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.083"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            632..1543
                     /locus_tag="ONT85_002537"
     CDS             632..1543
                     /locus_tag="ONT85_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-relaxation family protein"
                     /protein_id="HCR2379481.1"
                     /translation="MKYLLHGYFIDYSTRNSSTLAILCLLHELRVVTRKQLYKLLNFD
                     RSISKQGVTNALVRLYEKQLIDRKKYGTEMYYYLTKEGHQSIGGYYTLPKVPEYNLNH
                     HLQINNYLIKGMALAKDHPHLKRIISERRTVYETKDLNPKTKGRKFFVPDFIFRFRDK
                     ENHEINWSFEIELTLKTRRRYREAIFPKYIRELRTRPESHLIYVTPSGAIQEELHAFK
                     RIFFKRETEKDASQQELFERLHIFSALEFEEGLKALLENDPAVNWRTVEREKIYPNGA
                     VQSRLNLFEESTENEEKPKVKFTPEVL"
     gene            2030..2296
                     /locus_tag="ONT85_002538"
     CDS             2030..2296
                     /locus_tag="ONT85_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2379482.1"
                     /translation="MTTKNEQLKEQARKELQERGLIIEGSFEGDFETYIGCYARPINK
                     PTALDPMNEQEVKEQEKHAVNGFPQDFTEWYEWEINNGKLENFL"
     gene            2368..2709
                     /locus_tag="ONT85_002539"
     CDS             2368..2709
                     /locus_tag="ONT85_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2379483.1"
                     /translation="MAKNTPYQVGDIFYSSWGYEQTNVTFWQIVKLTEKTAWFQPIQK
                     KTVEHYSSMSGKVVPMKDKFIEYPLAKTKDSIVKKRIDPETYPDMLCGYRSWDTFHRY
                     KNQAVYESSYY"
     gene            2764..3183
                     /gene="ssb"
                     /locus_tag="ONT85_002540"
     CDS             2764..3183
                     /gene="ssb"
                     /locus_tag="ONT85_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HCR2379484.1"
                     /translation="MMNSVNLVGRLTKEVDLKYTPKGNATGTFILAVNRNYTNANGER
                     EADYIRCVIWRKAAETLAKYTGKGSLIGINGRLQTRSYQNQQGQTMYVTEVLVTDFYL
                     LESKEINEQRSKSMANNQNNSSIPGNEIQISDSYLPF"
     gene            3260..3556
                     /locus_tag="ONT85_002541"
     CDS             3260..3556
                     /locus_tag="ONT85_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2379485.1"
                     /translation="MKIIDSYDQRTMQYLGSFDYTDENIINYVASLPPYQSVRLVDFA
                     SDELVLTTMGNFLDYVGDKKWLEKILSKLIAKQTGEEPINAIQLFNRYEQENND"
     gene            3571..4002
                     /locus_tag="ONT85_002542"
     CDS             3571..4002
                     /locus_tag="ONT85_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HCR2379486.1"
                     /translation="MKRLKRSIFIVLALIDFVGTGCEAIDVQKAPLSEIKQAITKQDE
                     EQPLNTQVEIVNQNKPSFSEEDFSLENGTRQFFSDLDRLNRVGVASALLHKSRMPTEE
                     RDRLYIKPTGWKQKKMKNGDYLYNRCHLIGYQRATSFPISM"
     gene            4184..4489
                     /locus_tag="ONT85_002543"
     CDS             4184..4489
                     /locus_tag="ONT85_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2379487.1"
                     /translation="MKLDMPGRYKLLRVRLFLIKLTNLRMYGSITDLYSKDIRLRMQR
                     TLSKSKNVGHEIRDGRLAVYLSRVGKTCLIDSDESGNATKRFTEEIEQLAELNIRYF"
     gene            4893..5381
                     /locus_tag="ONT85_002544"
     CDS             4893..5381
                     /locus_tag="ONT85_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HCR2379488.1"
                     /translation="MKKTIFVLAIFICFGLLLVGYLLFNSNEMRTISTNDLLKKSELK
                     DKKKESLAFIMFTQEECEYCIAINPIIDSVNKETLNKIKKYDISVDFQSKQKCKIKFV
                     PTIVAYSKNKEVGRIDNELDNLYRKKQDGSIVIDKKKTKTLLIDWMKKMDKQGEYLYG
                     ED"
     gene            6715..7017
                     /locus_tag="ONT85_002545"
     CDS             6715..7017
                     /locus_tag="ONT85_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR2379489.1"
                     /translation="MTKLGITLDDNIYDEKILLSGGEKQKISLARALIKSASVIILDE
                     PFRNLDNETRDNLRDYILNTKKEVCYIIITHDHILDNFLDSNDEILYLNKEYDNNE"
     gene            7010..8038
                     /locus_tag="ONT85_002546"
     CDS             7010..8038
                     /locus_tag="ONT85_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HCR2379490.1"
                     /translation="MNSKKLQMGEIVRGPSQISIDVTYKCNFRCKHCYNASGGDNCFM
                     DNELTDDELLELTKDICNLKPYNICFCGGETLLRKKIVLSCATLLKEAGIPNIAIVTN
                     GSLVTQDVAKELFKAGINRFQVSLDGKNASSHEALRLYPNSFEKAINAIKLFRELSEE
                     VDVAFCPTSFNYNQLREVYQLVNTLKVKSLRIQPFMLSGRGMQNREIVPTVSQYRKLV
                     SDINYIKRNEDYTKIDWGDPIDHIIRHRTLLKDFSNFVSIRSDGGLMASPYLPIQVGN
                     IKCHSLSKYWRAGLYKVFSLDLVQDIATQVSSIQDMSKELKKGHIKWLNEDIEIDILD
                     EYKHDILI"
     gene            8094..8312
                     /locus_tag="ONT85_002547"
     CDS             8094..8312
                     /locus_tag="ONT85_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2379491.1"
                     /translation="MISEPQMNNMYESPTIMGAGEAAPRACSAIMVCVLLASAGVGIQ
                     AGAVYTYAVVATVAVAAAAVWKTVGLVG"
     gene            8383..9198
                     /locus_tag="ONT85_002548"
     CDS             8383..9198
                     /locus_tag="ONT85_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2379492.1"
                     /translation="MKYNNLKPKLLNENIEYIREENYGYLTIVPKNGTELKDNVFNPT
                     VRYILANCTGKNSVKKIANGLKKKFPSLEYEKFYQDVVGTIKFFKHYNIIDIGDYSME
                     EKKIYKIEQNLELELLNESHYEEIYNFITDHPESMYFHEDIYHQGNSKELEVVLRYNL
                     FSFTSEFYFLRHKERIVGMVSIIVNPFQNLSYSIVNYLICEPAYTTTILTTVLENINE
                     VSILEVKKLVIHLLNSTEDESLIPYLKDSHFFKEAVLSKEYREHDVLRYAYIY"
     gene            9262..>9444
                     /locus_tag="ONT85_002549"
     CDS             9262..>9444
                     /locus_tag="ONT85_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HCR2379493.1"
                     /translation="MILLILSLVLLVVSNLMKNNPPTKINSFYGFRTKKSMKNEKSWH
                     LAQKLLGDFSKKKVIPV"
BASE COUNT         3566 a         1238 c         1787 g         2853 t
ORIGIN      
        1 gggagggctg tcggcgcttg gtatgaaagg cattctccga cttccaggtt tttgaccatt
       61 ttctctcaac gtggaaaaac cgccacttat gagagaaaat ggtcaaaaac ctaagcggga
      121 agtctccgaa taacatacca agcgccgaca gccaacagat aaagaataca ctactaaaaa
      181 tagtagtagc ataaaattta aaagggaaaa aatcaacccc taccccagtc ttggggtctc
      241 aaaactaact cccccctaac cccctctttc agcctctttt gaaagagggg gagaatcaac
      301 cccacggcca acgccccagt gttcttgggg ctgtccagaa cagaaacttg cgagagagtg
      361 ggggtaaaac aagtgtagag gaaacttgaa gacttgttcg aacagaaact tgcgagagag
      421 ccggagtaaa acaagtgtag aggaaacttg aagacttgtt cgaacagaaa cttgcgagag
      481 agttggagta aaacaagtgt agaggaaact tgaagacttg ttcgaacaca aacttacaag
      541 agaggggagt aaagcagttg cagaggaatc aggaagattt gttcgaacag aaacttgcaa
      601 gaaagcatta ttaaattgaa ggtgatgaaa tatgaagtat ttattgcatg gctattttat
      661 tgattattct actcgcaata gtagtacttt agcgattcta tgtttgcttc atgaattgag
      721 agtggttact agaaaacagc tatacaaact attgaatttt gacagatcaa ttagtaaaca
      781 aggagtaacg aatgctttag tgagattata tgaaaaacag ttaatcgatc gaaaaaaata
      841 tggaacagaa atgtattatt atttgacgaa agagggacat caaagcatag gtgggtatta
      901 tacgttgccg aaagttcctg aatataattt gaatcaccat ttacaaatta acaattattt
      961 gattaaagga atggcattgg caaaagacca cccacattta aaacggatta tttcagaaag
     1021 acgtaccgtc tatgaaacga aggatttaaa tcctaagaca aaaggaagaa aattttttgt
     1081 gccagatttt atttttcgtt ttcgagacaa agaaaatcat gagatcaatt ggtcctttga
     1141 aattgagcta acgttgaaaa cacgtcgtcg ctaccgtgaa gcgatttttc cgaaatatat
     1201 tcgtgagttg cgaacacgtc ctgaatcaca tttaatttat gtgacaccta gtggcgcaat
     1261 tcaagaagaa cttcatgcgt ttaagcgtat ttttttcaag cgcgaaactg aaaaagatgc
     1321 gagtcaacaa gaattgtttg agcggttgca tattttttca gctttagagt ttgaagaggg
     1381 attaaaagca ttattagaga atgatccagc agtcaattgg cggacagtag aacgagaaaa
     1441 aatttatccg aatggcgctg ttcaaagtcg tttgaattta tttgaagaat caactgaaaa
     1501 tgaggaaaaa ccaaaagtga agtttacgcc tgaagtattg taaaagaggg tgttcgcttc
     1561 gctctttttt tgtgccagtt tgctggcacc gttcagacgc aagcgtctga accggctcgt
     1621 ttttaggaag gactaacttg tttgatttat ccgttgtcaa agaagtggac gttcgagagt
     1681 ccctaatcgt atagcagtca ttcggcgagt ataacctaaa acataacctg ccaattgcca
     1741 agggtaaaat gcacgctgcg cgcccttggc aatcgtcaga acatgttttc gagccggtta
     1801 tgccgaacga ccgctatacg aaaagggcct ctaaacagaa gtccactttg atccttgaca
     1861 actgaaaccc aaaatcaaac aagcaggcct tcgtctaaaa acgagccgaa tgaagacctt
     1921 gggggttgca cccccaaacc cccgtgcccc cgacacacta ccgaaaaggt gggggtgggt
     1981 ggcgaaaaaa tcaaataaaa cattctattc aagaaaggaa gaaacaacca tgacaactaa
     2041 aaacgagcaa ctgaaagaac aggcaagaaa agagttgcaa gaaagaggtt tgattattga
     2101 ggggagcttt gaaggagatt ttgaaaccta tattggttgt tatgcaagac cgataaacaa
     2161 acctacagcc ttagacccaa tgaacgaaca agaagtaaaa gaacaagaaa aacatgcagt
     2221 gaatggtttt ccgcaagatt tcactgaatg gtatgagtgg gaaattaaca acgggaagct
     2281 tgaaaacttc ttataaaagt cagatgacgt gcaaagagaa tgattctctt tgtacggtaa
     2341 aaatataaag aaagaaggaa aaagaaaatg gcaaaaaaca caccttatca agtaggagac
     2401 attttttata gtagttgggg ctacgaacaa acgaacgtga ctttttggca aattgtgaag
     2461 ttaacagaaa aaacagcatg gtttcagcct attcaaaaga aaacggtcga gcattattcg
     2521 tccatgagtg gaaaagttgt tccaatgaaa gacaagttta tagagtatcc tttagcaaaa
     2581 actaaagaca gtattgtaaa aaaacgaatt gatccagaaa cctatcctga tatgttatgt
     2641 ggttatcgta gttgggacac gtttcataga tataaaaatc aagctgttta tgaaagtagt
     2701 tattattaag gggatagttt cccctttcaa aaataataaa tagaagaatg gagagaaaaa
     2761 atcatgatga attcagtgaa tttagtagga cggttaacaa aagaagtgga tttaaaatac
     2821 acaccaaaag gcaatgcgac aggcacattt attttagctg ttaatcgaaa ttatacgaat
     2881 gcaaacggcg agcgtgaagc agattatatc cgctgtgtca tttggcggaa agctgcggaa
     2941 actttagcaa aatatactgg aaaaggaagt ttgattggca ttaatggacg tcttcaaacg
     3001 aggagctatc agaatcagca aggacagacc atgtatgtta cggaagttct agtgacagat
     3061 ttctatttgt tagagagtaa agaaatcaat gagcaacggt caaaatcaat ggcgaataat
     3121 caaaataatt cttcaattcc aggaaatgag attcaaattt cagatagtta tttgcccttt
     3181 taagtgaaaa ataattttgt tctatacctg atttcttcag gtatagaaca aaaaataaat
     3241 agagatgagg cgatttttta tgaaaatcat tgacagttac gatcaacgaa ccatgcaata
     3301 tttgggctcg tttgattata cagatgaaaa tattattaat tatgttgcaa gtttacctcc
     3361 ttatcaaagc gttcgcttgg tagattttgc aagtgatgaa ttggtattaa caacgatggg
     3421 caacttttta gattatgtcg gagacaaaaa atggttggaa aaaattttgt ctaaattgat
     3481 tgcgaaacaa acaggagaag aacccataaa tgcgattcaa ttgttcaatc gatatgaaca
     3541 agaaaacaat gattaaagag gagagaaaaa atgaaaagat taaaacgaag tatttttatt
     3601 gtgctagcat tgattgattt tgttgggaca ggttgtgaag caattgacgt tcaaaaagct
     3661 cccttatcgg aaataaaaca agcaataaca aaacaagatg aagaacaacc gctaaataca
     3721 caagtagaaa tagtgaatca aaataaaccg agtttttctg aagaagattt ttcgttagaa
     3781 aatggaacca ggcaattttt ttcagatttg gatcgtttaa atcgggtagg tgtagcaagt
     3841 gcacttttgc ataaaagcag gatgccaaca gaagaacgag accgattata cattaagcca
     3901 actggctgga aacaaaagaa aatgaagaat ggcgattatc tttataatcg ttgtcatttg
     3961 attggctatc aacgtgcgac aagtttcccc ataagtatgt aatacgaaac gggaggatta
     4021 atcttaagat taatcacaac taacctattt gacagttgga atgaaagcta tcatgtctgc
     4081 tgaatcaact ggtctgatac tcctacatgc aataaagtct actaggaagt tattgtatga
     4141 agctaggtaa aggcggcaga acaatttcta agtgtggaat catatgaagt tggatatgcc
     4201 gggacgctat aaattactca gggtgagact gtttttaatt aaactgacga acttgcgaat
     4261 gtacgggtct ataaccgact tatattcgaa agatataaga ttgcgaatgc aaagaacatt
     4321 atcgaaaagt aagaatgttg gtcatgaaat tcgagatgga agactagcgg tgtatctgtc
     4381 aagagttggt aaaacatgtt tgattgattc agatgagagt gggaatgcga caaaacgttt
     4441 tacagaagaa atagagcaac tagcagaact gaatatacga tatttttaaa tactttagcg
     4501 gagaataaaa aaatcctcta cagaaaacta cggagggcaa catgtgctat tgtttcaaca
     4561 atacattttt aatttttacc ataacaaaaa agtacttgaa aaaaaacaga aatatgttaa
     4621 attaaaaatg aaaaaatgga agcggttact tattcttttg aatctaagat aaaaattcaa
     4681 aagaataaca taacccaaat atgattatac ttgagattct aaatatttat cgaaaaggaa
     4741 tttgttttaa ggaaaaatga ttctatggac ggttattgat ttatatttac caaatggcta
     4801 ataaaatagt atttcttata ttgtatagtt tttgttttta tgtgaaaaca tgagcaatag
     4861 aattgtacta gtaggataag gatggatgga aaatgaaaaa aactattttt gttttagcga
     4921 tttttatatg ttttgggcta cttttggtag gctacttgtt gtttaattct aatgaaatga
     4981 gaactatttc tacgaatgac ttgttaaaaa aaagtgaatt aaaggataaa aaaaaagaat
     5041 ctcttgcctt tataatgttt actcaagaag agtgtgagta ttgtatagca ataaatccaa
     5101 tcatagatag tgtaaataag gaaactttga ataagataaa gaaatatgat atatctgtag
     5161 attttcaatc taaacaaaag tgtaaaatta agtttgttcc gactattgtt gcatatagta
     5221 agaataaaga agtggggaga attgacaatg aattagataa tttgtatcga aaaaagcaag
     5281 atggttcaat tgtaatagac aagaaaaaaa caaagacatt gcttatagat tggatgaaaa
     5341 aaatggataa acaaggagaa tatttgtatg gagaagatta aacagacatt tccgttaatt
     5401 aataaatatt ggaataagaa aaaaattttt atatttttta ttacgtttct gctgtcatct
     5461 ttattttctc taattttccc gtatctattt agtaatgtta ttgatttata tttagagaat
     5521 gagttatcac agaatattgt gcgaatagta acgattctta tatttcagtt atttttagtc
     5581 ttaattaaag aagtgtatgg atatataaaa actatttatg catttgaagt tgatttatca
     5641 atgaaaattg attttcttaa ggttttacaa aaaaaaaatc tttttttttc gcaaacgaag
     5701 cattcaggag aaattcaatt taggatgttt tcggatattt ctactatcgt aaatactatc
     5761 tttatttttt ctgttgaatt acctgtgata ggcgttttag ttcttgtagc ggtatttatt
     5821 ataagtaaag tgaatcttat ttttctgatt ttttcactaa taatgatggg agctatggtg
     5881 ttacagtata atttatttca attaccgctt aagaaaacaa tcgagaatac aaaaagtgtt
     5941 aatcaaagta ttacaggaga attgaatgca cattttgatc aattagaaat tataagcgca
     6001 tataattttg agaatatctc tattttaaaa ttcgattcta gatttaaaga attatttaat
     6061 gttttcaaaa aacaaaataa attactaaca ctgggaagct gtttggcaca ggttattttc
     6121 caattatgga ttggaggaat atttcttatt gatgttattt tattagctaa aggaacaata
     6181 agtattacta attttgtaat agtatttatg tacgctaaca tggtccctac tttatggact
     6241 actttaatta gtattatttg gaaatggcaa gactattcag tgtcattaaa tagatattac
     6301 gaatattatt caaataacaa caaagtggct ggcacaaaaa aaataaataa aataaattct
     6361 attactggtc aatcattaaa tttttcatat aacaaaagaa aggtattaag caatataagt
     6421 ttttcaactg aaatgaataa actattcatg atatcaggag aaaatggaac agggaaaaca
     6481 acactgatga aattatttac taaacagttg ccggttagta ataatcaatt attcgtcaac
     6541 gggatagata ttaatatgat tgataacgaa tcattaagaa ggttaatttc aatcgcaaca
     6601 caaataccat atataattaa tagctccgtg aaggagaata ttttaatggg gactaaaaac
     6661 tatgataaaa aaaaattcag taaattaata gaactattgg gaataagtaa atttatgacg
     6721 aaattaggta ttactttgga cgacaatatt tatgatgaaa aaatattatt atctggagga
     6781 gaaaaacaaa aaataagttt agctagggcg cttattaaat cagcttcagt tattatatta
     6841 gatgaaccat ttagaaattt agataatgaa actcgcgata atttgaggga ttatatacta
     6901 aatacaaaaa aagaagtttg ttatataatt ataactcatg atcacattct agataatttt
     6961 ttggattcaa atgatgaaat attatattta aataaggagt atgataataa tgaatagcaa
     7021 aaaacttcag atgggtgaaa ttgttagagg tcctagtcaa atatctattg atgttacata
     7081 taaatgtaat tttagatgta aacattgtta caatgcgagt ggtggagaca attgttttat
     7141 ggataatgag ttgactgatg atgaactgtt agaacttaca aaagatatat gtaatttaaa
     7201 gccatataat atttgtttct gtggaggaga aactttactg agaaaaaaaa tagttttatc
     7261 ttgtgccact ttattaaaag aggcaggtat tcctaatatt gctattgtta ctaatggatc
     7321 attagtaact caggatgttg caaaagaatt attcaaagca ggtattaatc gatttcaagt
     7381 aagtttagat ggaaaaaatg ctagtagcca tgaagcactt agattatatc caaacagttt
     7441 tgaaaaggca attaatgcta tcaaattatt tagagaattg tccgaagaag tggatgttgc
     7501 tttttgtcct actagtttta attacaatca attacgagaa gtatatcaac tagtaaatac
     7561 tcttaaagtg aagtctttaa gaattcaacc atttatgctt agtggtaggg gaatgcaaaa
     7621 tagggaaata gtgccaactg tttctcaata tagaaaacta gtttctgata ttaactatat
     7681 taaaagaaat gaagattata ccaagataga ttggggagat ccaattgatc atatcatccg
     7741 acacagaact ttattgaaag atttttcgaa ttttgtttct attaggtcag acggaggatt
     7801 aatggcatca ccttacttgc caattcaagt tggaaatatt aaatgtcaca gtctttctaa
     7861 atattggcga gcaggacttt ataaagtttt ttctttagat ttagttcaag atatagctac
     7921 tcaagtttct tcaatacaag atatgagtaa agaattaaaa aaaggacata ttaaatggct
     7981 taatgaagat atagaaattg atattctcga tgaatacaaa catgatattt taatttaggt
     8041 ttatcattgg aaagtttaga taaacaaagt acttttagaa aggagggaaa aaaatgattt
     8101 ctgaacctca aatgaataat atgtatgaaa gcccaactat aatgggagct ggtgaagctg
     8161 cgccaagagc ttgttcagca attatggtgt gtgtgttatt agcatctgca ggagtaggta
     8221 tccaagcagg agcggtgtat acttatgctg tagttgctac agtagctgta gctgcagccg
     8281 cagtttggaa aacagtagga ttagttggtt aggaattaat aggagactaa ctgtctccta
     8341 ttgattccaa tgattaagct aaggaggaat tttaagaaaa taatgaaata caataattta
     8401 aaaccgaaat tacttaatga gaacatagaa tatataagag aagaaaatta tggatattta
     8461 accatagttc ccaaaaatgg aactgaattg aaagataacg ttttcaatcc aacagtaaga
     8521 tatatattgg ctaactgtac tggaaaaaac agtgtaaaaa aaatagcgaa tggtttaaaa
     8581 aagaagtttc ctagtttaga atatgaaaaa ttctatcaag atgttgtagg aactattaaa
     8641 tttttcaaac actataatat tattgatata ggagattata gtatggaaga aaagaaaata
     8701 tataaaattg aacaaaattt agaattagaa ttactaaatg aatcacatta tgaagaaatt
     8761 tataatttta tcacagatca tcctgaaagt atgtattttc atgaagatat ttatcatcaa
     8821 ggaaatagta aagaattaga agttgtcctt agatataatt tattttcgtt tacgagtgaa
     8881 ttttactttt taagacataa agagagaatt gtaggaatgg tttctataat tgttaatcca
     8941 tttcaaaact tatcctacag tatagtaaat tacctcatat gtgagcctgc atatactacg
     9001 actattttga caacagtatt agaaaatatt aacgaagttt caattttaga agtaaagaag
     9061 ctagtgattc atttgctaaa ttcaacagaa gatgaatcac taatccccta tttaaaagat
     9121 tcacattttt ttaaagaagc ggtactatct aaagaatata gagagcatga tgttttgaga
     9181 tacgcttaca tttattaaat aaaatttctg atagtatttt aataattcgg atcatatcat
     9241 aagatatagg agggaaaaga agtgatttta ttgattttgt ctctcgtatt actagttgta
     9301 tctaatctaa tgaaaaataa tcctcctaca aaaattaatt ctttttatgg ctttagaact
     9361 aaaaagtcaa tgaaaaatga aaaaagttgg catttagcac aaaaattgtt gggggatttt
     9421 tcaaaaaaaa aagttatccc agta
//
LOCUS       DAJSBL010000082         9213 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.084, whole genome shotgun sequence.
ACCESSION   DAJSBL010000082 DAJSBL010000000
VERSION     DAJSBL010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9213
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.084"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            4..2376
                     /locus_tag="ONT85_002550"
     CDS             4..2376
                     /locus_tag="ONT85_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2379494.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQRKTNGQ
                     PMNDKRNGHSWFRQQERLENKVHSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIENIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKERSEKGKENLLPEVQAI
                     IDSGRVTQWKKNPTIYFLKDYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     APTDSLKEKENDHFYRVEFNENDPEKGLKSYGGEIVTLELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQTVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKKGQSELQAGIQAVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTVETAEQKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNSFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            2437..3897
                     /locus_tag="ONT85_002551"
     CDS             2437..3897
                     /locus_tag="ONT85_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HCR2379495.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDPKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKHERQNF
                     LVRDDRLDHAVAFLWKDPQTNETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQLKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKVI
                     YEEVAKEEKITPEAIMAIHKTENNLQLTNQLVSAHKVNASFGQQLSVNDSIEAINLKD
                     ICREVAKELKACERVDGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFVPE
                     VKKDWNDQLKHEIYQQEIRKQKRAMLFQQGRQQERE"
     gene            3908..6112
                     /locus_tag="ONT85_002552"
     CDS             3908..6112
                     /locus_tag="ONT85_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HCR2379496.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFSGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFKVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQAGADHGKIFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAQVIAQNGAFDAKLDPNETQATQEAFEDYLKEKGVQLGKQPGTIHQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAEAIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     LSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKTVGKVPVDLGWKKLFGK
                     ESDRKEKEEEPLLPKVTKGEAVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLTKIGEQKGTQATFLANIQKFVSHLLEVVPGQIQATDFGATLQEVKV
                     ASEKQEAARHLGSCPKCQEKEVILYQKVATCTSEACDFKLWVTIAKKKLTATQLKEII
                     QNGRTSQPVRGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTTYKRKVRRKTS"
     gene            6133..6282
                     /locus_tag="ONT85_002553"
     CDS             6133..6282
                     /locus_tag="ONT85_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2379497.1"
                     /translation="MRNTTHKEQEIENQKAKIRSEQEKMRASKRRLQQLKNQQRIQKE
                     LELDR"
     gene            6358..6951
                     /locus_tag="ONT85_002554"
     CDS             6358..6951
                     /locus_tag="ONT85_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HCR2379498.1"
                     /translation="MKDKVKCIFEKYNGNLSLKDARNEGLSPMTLDRLAEKDKIEKIA
                     SGFYTLKDSFVDELYLAQSIYTRGIFSHETALDLFQVGTYIPKKINMMFPKGYNVSKE
                     NLDQYAIQPHYTNKENFELGITEAESFYGNKIFVYDLERTLCDMWNPRYKASVEVKQE
                     ALKEYMTSTSRNTNKLRRYMNILKSPKEMTLYMLPLY"
     gene            6967..7866
                     /locus_tag="ONT85_002555"
     CDS             6967..7866
                     /locus_tag="ONT85_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HCR2379499.1"
                     /translation="MTPTQFKAKTRNVAAEKHLNPQIVQRYYMMEKLLECIVESPYHD
                     DFVLKGGFLIGSKYGLENRSTVDIDTTYRNSKLTKEKLETVFSELTAAPTKDGIRFEL
                     KGLTETREADYYPGFQAKFIAFLENARVPFKLDITSGDSIIPAALIYEHKLMFEDKTI
                     HIPAYPTEQILAEKLHATFSFGIDNSRMKDFYDLYMIPKLERIDEINLYKSVQGTFKE
                     RNNQSVFKNLYNQEMPQLMKNEGLREKWKRYQQENYFAKDISFESTLTSVDSLMQSIV
                     EQEEVERKQNFKRSQQMNQEMER"
     gene            7869..8354
                     /gene="ssb"
                     /locus_tag="ONT85_002556"
     CDS             7869..8354
                     /gene="ssb"
                     /locus_tag="ONT85_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HCR2379500.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVTTFTLAVNRNFTNQSGNR
                     EADFINCVIWRKPAETIATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRMHADQSSSSPSPTTFEQRDTATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
     gene            8720..8980
                     /locus_tag="ONT85_002557"
     CDS             8720..8980
                     /locus_tag="ONT85_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2379501.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIASMYVRNPDHFTRSMGI"
BASE COUNT         3464 a         1547 c         1926 g         2276 t
ORIGIN      
        1 gacatggcag aagaaaaaga accactagac aatagtcgct tgggaaaatc caaaagaaaa
       61 ttagtacgat tgcaaaatga attaaacgaa caaattgaga agatgtttga acaccagaga
      121 aagaccaacg gtcagccaat gaacgataag agaaatgggc attcttggtt taggcaacaa
      181 gaacgtttag aaaacaaagt gcatagtctg agagaagaga tcaagcaaca agaaaaacag
      241 gtagaaaaac ttgaaagaca agaagagcta aaggaaatgg gctataacaa gtacggcggc
      301 ctggatatga caatcgaaaa tattccaaga atcaaagaag aaatcgaacg ctttgaaaaa
      361 ggtgaatcga ctttttcagc cgctacgatc cgaaaatacc aacggaaatt ggaaacatta
      421 gaacagctga aagaaagatc agaaaaagga aaagaaaatc ttcttcctga agtacaagca
      481 attattgaca gcggacgagt gacgcaatgg aaaaaaaatc caaccattta ttttttgaaa
      541 gactatcgaa aagttgcttt agaactgaat gaaaacgggg gatttgagga atcaaaaaaa
      601 taccaagcca cgtcggaaga ggaaaaagca gctgttcgtg aattacttga agaggctgcg
      661 ccaactgatt ctttgaagga gaaagaaaac gatcatttct atcgagtaga attcaacgaa
      721 aatgatccag aaaaaggatt gaaaagttat ggtggggaga tcgtaacatt agaattgatc
      781 gatcaactca aaacgttgga taaccaatac gccgcagaag caggttatta taaattctat
      841 tttgaagaag tgaagaacgg agaagtagtc gatcagatcc gaatggatct aggggatggc
      901 gtcaacagta atcaacccat ctatttagag ttagcaaaat tgtgcgaaca agtacctgaa
      961 aaggaacaaa aacaaacggt caaaaatgaa cctgtttcga aagaacaaat gcagtcgaaa
     1021 gaaacaaaaa caaaacaaaa gggctatcaa aaacgaacgg cagaagagat taagcaagaa
     1081 gtcaaatccc tttcgcaaca ggcactggat gcggtgaaaa agcataccca ttctcctgaa
     1141 gacgtgaaag aacttttgaa ttttatgtct cgttttccag aacgatcctt tagaaatcaa
     1201 ttattgattg aggaacaatt tccaggtgca acggcttgtt taggtcgtgc tgcgttgaaa
     1261 aaagaaggaa tctatatcaa aaaaggggaa caagggaaca agatctttgt cagaaaaacg
     1321 gtcaaaggat tttatgagaa aggacgtggc tttgttcgag aaacagaagc tacaccagaa
     1381 gataaaaaac gaatcgaaag tggtcaaatc gaagtgatca aaaaacctta ctacacgatt
     1441 gaaaaagtgt ttgatatcac acaaacacag ttaaaacctg aagactatcc gaaaatcttt
     1501 ccgaaccgtg tctttgattt tcaattggat aaaaaaggac agtcagaact tcaagctggg
     1561 attcaagcgg tagccaacaa tattggaata caaattcgag atatgacaga aagtgaagtc
     1621 tatcaaagag aattaggaca agcacggggg gcatatgttc gtaacgaatt tactggcgaa
     1681 gaagaaatcg tcatgaatac tcgaaatact cctacgcagc atttagctac aagtattcat
     1741 gaattagccc atgcaagaat gcacaaattt tcagaattag ataccgcaac taaagaattg
     1801 caggcagaaa tgacttctta tattgtgtgc aaacattttg gaatggacac ttcggagaaa
     1861 gctattcctt atattgcagc atggacgaag aatggtcaga cgttggatga taaggaagct
     1921 gcggaacgtg ggaaaatcat gaatgatgtc agccgtgtcg caaatgaatt cattcaaacc
     1981 atttctaatg aaatcaatca atacagagaa agagaaccag agatccaacc agcagaagtg
     2041 aagcaggagc ataaaaatac tgtagaaacg gcagaacaaa agaatattgt tcctgttggt
     2101 tcactttgga tcgacaagaa agacgggaaa gtaatggaag aagatacggg tcgaacgtta
     2161 catttgaagg atgcggcgtt tattgaagat ccacagcata atcaagtaat tttaaaagga
     2221 aaaagttatg gaaaagagat ccaaacggcg ttaccttcag aagaatttgc gctaggaaat
     2281 tcattccgat tttcacttga gacagtgtca gaagtggcaa aacgaaaaaa tgaaatctat
     2341 tatgaacaac aattgtcaca aaaagaacgt gtataaaaca agtaaaaaag actatctgaa
     2401 aaacggatag tcttttttct tttgggaggt tttaaaatga ccgagaaaaa aatgagtctt
     2461 attgatcgat gtaaacagat cgatatagtg gattttgctc gaaacaatgg aatggctgta
     2521 gtcaataaag gacgagacta tcgcttagaa gaccatgatt catttgtttt tgatcgaaga
     2581 aaacaacgtt tttattggaa cagtcaaaat attagtggcg acattattga attagctaaa
     2641 ctttttttca tagacaaaga gatccaagac cctaagcaac aatttaaagc agccactgat
     2701 tttattctaa agaatgaaga caaaacagaa cgagtggaaa atcttcattt tgagacagaa
     2761 aagtacaaag atcaccccgt tgattatcaa ccactcacag aaaaaggaag gaattatctg
     2821 aaggaagaac gaaaactacc agagtggctg attgattatg cagaaaaaga agggttgatt
     2881 gccgaactaa agccaaaaca tgaacggcaa aatttcttag ttcgagacga tcgtttagat
     2941 catgcggtcg cctttttatg gaaagatcca cagacaaatg agacagtagg ggcgagttat
     3001 caaggaacgt tcattgatta tgaacgtttt ggtgagagag gcacatataa gcatatagat
     3061 aaaaattcga cagcgaatca tggctttaac ctaaagatcg gtgaccctaa gcagttaaaa
     3121 ttttttgaga gtagtattga tttacttagc tatgctgcac taaatcgtga tcagttaaac
     3181 gatacgtggt tagtttcgat ggaagggtta aaacaccatg tgatcagtca ttattttggt
     3241 gaggcagtat ctgaattgag aaagaaacaa gcctttcctc aatccatcga gatttgtgtg
     3301 gataatgacc gtgcaggaca tattttctac gaaaaagaac aattaatggg cgcagttgat
     3361 ccatttacga atcagaaagt acgttgtgaa cgaggaatcg ctaatgattg gcaagtgcca
     3421 aaagaataca aagtcattta tgaagaagtc gcaaaagaag aaaagatcac accagaagct
     3481 attatggcca tacacaagac agaaaacaat ctgcaattaa cgaaccaatt ggtttccgct
     3541 cacaaagtaa atgcgtcttt tggtcagcag ctttctgtta acgattcaat agaagctatt
     3601 aatttgaaag atatttgtag agaagtcgca aaagaattaa aagcttgtga acgcgtggat
     3661 ggtacgtacg attttgatcg tttttatcaa gaaaaagggg atattaatgc tcagattctt
     3721 ttctcttata aagcagaaca atactacaaa ggctataaaa atcatgaaca tgaatttgta
     3781 cctgaagtaa aaaaggattg gaatgatcaa ttgaagcatg aaatctacca acaagagatc
     3841 agaaagcaaa aacgtgccat gttgtttcaa caaggtagac agcaagaaag ggagtgaaat
     3901 cgtcatgatg aaaaccgtta ttttagcaga aaaaccgtca caagcgaagg cttatgccga
     3961 tagcttttct aaagctaccc gaaaagatgg gtattttgag atccaagatc gattgttttc
     4021 aggagaaact gtgatcacgt atggttttgg tcatttagtc gagttagatt cgcccgatat
     4081 gtacgatgaa aattggaaac aatggtcatt agagcatttg cctattttcc ctacacatta
     4141 tcactatcat gttcctaaag ataagaaaaa gcaattcaag gtggtgaaac agcaacttca
     4201 atcggccgac accattatta ttgcgacaga tagcgatcgt gaaggggaac tgatagcttg
     4261 gacgatcatt caacaagcag gcgccgatca tggaaagata tttaaaagac tttggatcaa
     4321 ctccttagaa aaagaagcca tttatcaggg attccagcag ttacgagatg cagaagagac
     4381 ctacccaaaa tttgaagaag cacaagcacg ccagatcgcc gattggttga tcggcatgaa
     4441 cggtagcccg ctgtatagtt tactcttgca gcaaaaaggc ataccaggaa gtttttcctt
     4501 aggcagagtc caaaccccaa cgctttacat gatttatcaa ttacaggaaa agattagaaa
     4561 cttccaaaaa gaaccatatt ttgaaggaaa agcccaagta atcgctcaaa atggcgcatt
     4621 tgatgcgaag cttgatccga atgaaaccca agcgacgcaa gaagcatttg aagattattt
     4681 aaaagaaaag ggggttcaat tgggaaaaca gccaggtacg atccaccaag ttgaaaccga
     4741 gaaaaaaagt gcggcaagtc cacgtctttt ttctctatcc agccttcaat ctaaaatgaa
     4801 tcagctcatg aaagctagtg ccaaagatac tttggaagcc atgcaagggt tgtatgaagg
     4861 gaaatatcta agctacccac gaacagatac cccttatatt acagaagggg agtatgcgta
     4921 tctgttggat cacttagacg aatacaaaca ctttttaaag gccgaagcca ttcctactcc
     4981 tattcataca ccgaatagtc gatatgtgaa taataagaaa gtccaggaac attacgcgat
     5041 cattcctaca aagacggtca tgacggcagc ggcttttgaa caattaagtc cactgcagca
     5101 ggctatttat gaacaagtgt taaaaacaac ggtggccatg tttgcggaaa agtataccta
     5161 tgaagaaaca acgatcttga cgcaagttca gcagcttcag ctaaagacag tcggaaaagt
     5221 accagtagat ctaggttgga aaaagctgtt tggcaaagag agcgacagaa aagaaaagga
     5281 agaagaaccg ctacttccta aagtgacaaa aggagaagcg gtgacggtag atctacaagt
     5341 ccttgaaaaa gaaacgaagc caccacaacc ttataccgaa ggtacgttga tcacagcgat
     5401 gaaaacagct ggaaaaaccg tggatagcga agaagcacaa tccatattga aagaagtaga
     5461 aggaatcggt acagaagcaa cacgagcaaa tattattgaa actttgaaac aaaaggaata
     5521 tatcaaggta gaaaaaaata agcttgtggt gacaaataaa gggattttac tttgccaagc
     5581 cgtggaaaaa gagccgctgt taacaagtgc cgaaatgacc gccaaatggg aaagctatct
     5641 tacaaagatt ggtgagcaaa aagggacaca agctactttt ctagcaaata tccaaaaatt
     5701 tgtttcccac ttgttggaag tcgtcccagg acaaatccaa gccactgatt ttggagcaac
     5761 gttacaagag gtgaaagtag ccagtgaaaa gcaagaagcg gcgcgtcatt taggatcttg
     5821 tccaaagtgc caggaaaaag aagtcatact ttatcagaag gtggcgactt gcacgtctga
     5881 agcctgtgat tttaaattgt gggtaacgat tgccaaaaag aaactcacag cgacacaact
     5941 gaaagagatc attcagaacg gccgaacaag tcaaccagtc agaggactaa aaggtcagaa
     6001 aggttcgttt gaagcaacga tcgttttaaa agaagacttt acaacaggat ttgagttttc
     6061 tgaaaagaag aagaccacct acaaaagaaa agtgagacgt aaaacgagtt agaattgacg
     6121 gagggaaaaa cgatgagaaa tacaacgcat aaagaacaag aaatcgaaaa tcaaaaagcc
     6181 aaaatacgtt cagagcagga gaaaatgcgt gccagcaaac gaagactaca gcaattgaag
     6241 aatcaacaac ggattcaaaa agaacttgaa ttagatcgat aaatcctcct gttgttaata
     6301 ttaaactaac atgttatagt tatgttagtt taatattaac attcgagagg gaaagcgatg
     6361 aaagataaag tcaagtgcat ttttgagaaa tacaatggta atctatcctt gaaggatgct
     6421 agaaatgaag ggttatcacc tatgacgtta gatcgtttag ccgaaaagga caaaatagaa
     6481 aaaatagcat caggttttta taccttaaaa gatagttttg tggatgaatt atatctcgct
     6541 caaagtatct atactagagg aatcttctct cacgagacag cgcttgatct ttttcaagta
     6601 ggaacatata tccctaaaaa aattaatatg atgtttccta aaggatacaa tgtttcgaaa
     6661 gagaatttag atcagtatgc cattcaaccg cattacacga ataaagaaaa ctttgaactg
     6721 ggaatcacag aggcagaaag cttttacggc aataaaattt tcgtctatga tttagagcga
     6781 acgttatgtg atatgtggaa tcccagatat aaagcaagcg tagaagtaaa acaagaagca
     6841 ttaaaagaat atatgacaag taccagcaga aatacaaata agctaagaag atacatgaat
     6901 attttaaaat caccaaaaga aatgactttg tatatgttgc cactttattg agaggagtaa
     6961 aggtgaatga caccgacaca atttaaagcg aaaacaagaa atgttgcagc tgagaaacat
     7021 ttaaacccac aaattgttca aagatattat atgatggaaa aattattaga atgtattgtt
     7081 gaatcgcctt atcatgatga ttttgtatta aaagggggat ttctgatcgg atcaaaatac
     7141 gggttagaaa accgttcaac cgttgatatt gatacgactt atcgaaattc gaaactaaca
     7201 aaagaaaaat tagaaactgt tttttctgaa ttaacagctg ctcctacaaa agatggcatt
     7261 cggtttgaat taaagggctt aacagagacg agggaagccg attattaccc aggttttcaa
     7321 gcaaagttta ttgcttttct tgaaaatgca cgtgtacctt ttaaattaga tattacttca
     7381 ggtgattcta ttattccagc agcattgata tatgaacata aattgatgtt tgaagataaa
     7441 acaattcata ttccagctta tcctacggaa caaattttag ctgaaaaatt acatgccacg
     7501 tttagtttcg gaatagataa ttcacgtatg aaagatttct atgatctgta tatgatccct
     7561 aaactagaac gtatcgatga gataaatttg tataagagtg ttcaaggaac atttaaggaa
     7621 agaaataatc aatcagtgtt taagaatttg tataatcagg aaatgcctca attgatgaag
     7681 aatgagggat tgagggagaa atggaaaaga tatcagcaag aaaactattt tgccaaagat
     7741 atttctttcg aatctacctt aacttctgtt gattcgctga tgcaatcaat tgtcgagcaa
     7801 gaggaagtag aacgaaaaca aaattttaag agatcacaac aaatgaatca agaaatggag
     7861 agataaagat gatcaataat gtaactttag taggtcgatt aaccaaagat cccgatttac
     7921 gttacaccgc aagtggcact gcagtgacca cttttacctt agccgttaac cgtaatttta
     7981 ctaatcaaag tggcaatcga gaagctgact ttatcaattg tgtgatttgg cgcaaaccag
     8041 ctgaaacgat agcgacatta gctaaaaaag gcagcttgat cggcgtcgtc ggacggattc
     8101 aaacgcggac ttatgacaac cagcaaggac agcgtgtcta tgtgactgaa gttgtggccg
     8161 ataatttcca actattagaa agtaaagcag cgacagaaag ccgtatgcac gctgatcaat
     8221 cgtccagcag tccaagccct accacctttg aacaacggga tacagcgacg cctaacaaca
     8281 atggattgaa cgcttcacag aatccatttg gtggacaatc aatcgatatt agcgatgatg
     8341 atctaccgtt ctaagtagca ttttctattg gttgcatttg ctacggttta tgttataatt
     8401 tagacaacaa aaagaatttt caatctataa ccaaaaacca ctagctggcg ggctagtggc
     8461 tttcttttac gtttctatta taagaattaa cgggtaggct tactgtcttt cttggcaaat
     8521 ttacaatcaa gccatttgca gatatagtgc gcggctactt ctgccatgat tgcaaaaaca
     8581 aaatcaaaaa gaattttcaa acctataacc tcctcccctt gccagattag gccggggtag
     8641 cagacagcaa gactattata gcataattga aatatccaaa tgattggatt tttgctataa
     8701 ttttagcgag gtgattgtga tgaacaaata tgttggtttg cttgataaaa tccgagtaat
     8761 aaaaacgcaa ccgttattgg ttcgctttac tttacaaacg attcatgaat cgattaattg
     8821 tgtagttgca gatattgaaa ttatcgataa attgctaatt atggatgatg gaaaatacaa
     8881 tattgccgtc acaggtcact tcaataaacg aaatcagcta gttattgcta gcatgtatgt
     8941 tagaaatccc gatcatttta cgcgatcgat gggcatataa ataaaagaag agttgtagtc
     9001 ctaaatgagg attgcagctc tttttgtttt acatagaaac aggaaaagac ctttttagga
     9061 agacacaagt gccttctcaa ttgaaaaacc tttctctcac tccaaccgcc accgccgttc
     9121 gagaagaaag gaagtgaacg ctgcacgttc actaagatat ggtgtcttgc aaagcaagac
     9181 aaaatagaca aaacttcctc tcaccaaaag gtt
//
LOCUS       DAJSBL010000083         8941 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.085, whole genome shotgun sequence.
ACCESSION   DAJSBL010000083 DAJSBL010000000
VERSION     DAJSBL010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8941
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.085"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            153..1367
                     /locus_tag="ONT85_002558"
                     /pseudo
     CDS             153..1367
                     /locus_tag="ONT85_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(1546..1875)
                     /locus_tag="ONT85_002559"
     CDS             complement(1546..1875)
                     /locus_tag="ONT85_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HCR2379502.1"
                     /translation="MNTNDIATVYVAFTAAEGGKRRPVYILKNGLDTISFLSITSKYH
                     TKSDKIKLKYVEIRDWKQAGLKKESWIDVGTLNELPKNNKKVKWKKIGELSPSDLQRL
                     EERMEEL"
     gene            complement(1865..2137)
                     /locus_tag="ONT85_002560"
     CDS             complement(1865..2137)
                     /locus_tag="ONT85_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HCR2379503.1"
                     /translation="MEKMAKLQISMDEKLKNEAESIIDELGMSPKTAVTVFYKQIVEQ
                     GKIPFSIELSEKSKRIHNIQRLAEKLPVQKLDTDEKIEEWFNEDEY"
     gene            complement(2588..3391)
                     /locus_tag="ONT85_002561"
     CDS             complement(2588..3391)
                     /locus_tag="ONT85_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HCR2379504.1"
                     /translation="MRFLHLSDIHFRENYQTNLFTAELFPTIKNPQLALEKLLQELSL
                     TVYDFILLTGDLVHEGGISDYQVLRKMLDQYFPTTPYYFCRGNHDRRSVFFEGMSVES
                     NETGEYIHCTNQNGLRIISLDSAQDTQHEGKISIGQMIQLQEWLGTPAEKGTILLVHH
                     PLSWEDPSIVTEVPEGFAELIAESDILGIFVGHIHQGSSAEFAGKKQFMAESLSFGVD
                     EYPDYSLFTNRTGFTSGSLIDGKIFTYQHYLTPHQIEIGRMKKPITGNH"
     gene            complement(3369..4493)
                     /locus_tag="ONT85_002562"
     CDS             complement(3369..4493)
                     /locus_tag="ONT85_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR2379505.1"
                     /translation="MGETLELRNIGKKYGEHTILKEIDLAVAAGERIVLLGPSGSGKS
                     TLLRMIAGLEKITSGELFLGTQLANKLPCGEREIAMVFQNYALYPHMTVKENIIFALR
                     ANKIKTADIQHRLEEALKMLGLTPYQDRLPKDLSGGQRQRVALARAVVKRSNYFLLDE
                     PLSNLDVRLRLDARKELVKIHQRYKQTFVYVTHDQIEAMTLADRIVLLNEGRIQMIDT
                     PKNVYTKPKNTFTANFIGSPGMNIMPVTYDQGTLKVASQFLTLNPAWQKQLLQVSGSL
                     YLGIRPEHVTFSKETSDLHGQVKYRELLGQSYAYTVKVDGIEMIVLNEKPTWQIGDAV
                     NLRIEPERMHFFDYQTQENLGYPDYFIEERGNSHALFASL"
     gene            complement(4509..5324)
                     /locus_tag="ONT85_002563"
     CDS             complement(4509..5324)
                     /locus_tag="ONT85_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCR2379506.1"
                     /translation="MKIKRWLPHLLFMFIIIIIIYPLLFIMANSFKPLKDAYQTITSL
                     IPQPFTLENFCYLFENLPIIKITRNTFFIATIITVVKLGVAFFAAYSFTYCRIKGKKI
                     WYFIIIGTMFIPFTVAMIPNYLLIAKLNLIDTTWGVIIPQFADAMGIFLLTQTMREIP
                     VSLLDVAALDNIKQTTVMSKIVFPLTRHAITSTGIWFFITSWNEYVWPVLILRTVDNY
                     TLPLAMQTYISSEGGTNFTVAMAISLITMFVPLVLYAIFQKYIIGTFVSAGIK"
     gene            complement(5314..6186)
                     /locus_tag="ONT85_002564"
     CDS             complement(5314..6186)
                     /locus_tag="ONT85_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCR2379507.1"
                     /translation="MKRYVGYLYLAPAIILLGVFVFYPLIYTIYLSFFDWNMVAPTKE
                     FVGFANYVKVLTDPVFHKVLKNTLSYTVLFVLLSCVFPYITSFLLGIVIEKGQNLYKP
                     LFFIPAVISLVVAGMVFTWILNPVSGPIAVLLGNFGLRLPMWMNLQGWVIAVIAGITA
                     WKVFGYNFIVIFAAIVGIDQEMIEAAKLDRVPIWRIFFTIVLPMSSSTGLYLLVMTIV
                     QGLQYVFTPIKIITRGGPNNGSANVIYYAYQKAFEMYKVGESSAISVLTLLIFAVFLG
                     VIFKFVEKGVYYEN"
     gene            complement(6271..7560)
                     /locus_tag="ONT85_002565"
     CDS             complement(6271..7560)
                     /locus_tag="ONT85_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR2379508.1"
                     /translation="MLKKNLVIGVVSVLSVISLAACGSGNTKTTKKSEGRVTIEYWHV
                     NADTLGGKTVNELVDKYNQSQDKVKVVAKYNPDMYKGLMQNLQASVASKEIPDVVQVG
                     WAFKDYFAENFKYTNPQSLVEEVDPDNADYFKDHFLDNIMALSEKDGKQVGIPYSISN
                     PVLYLNQDILKAAGLDENGPKTWEEVQTFSKIIKEKTGKYGVYIQEPADNWAQQAILE
                     SNGASVIKDGKATFASKDGIAAYELYQRMVKDQTALHTTWEQGVQSFIDGNVAMVYTT
                     IAQRDNIQKNSTFTAKAVESPSWEGKKVKLPAGGAMLAVTSQTDKKKEATWDFLKYLY
                     SVESLAKWTEGTGYLPPRKDVADAQNGLKAFLAENQMMIPAIDQMDKMVSWSSFPGDA
                     GLEAEQKLLDMRDKILSGNSVNETMKATQKEINELLK"
     gene            complement(7788..8432)
                     /locus_tag="ONT85_002566"
                     /pseudo
     CDS             complement(7788..8432)
                     /locus_tag="ONT85_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303552.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
BASE COUNT         2778 a         1896 c         1426 g         2841 t
ORIGIN      
        1 aaaagtgagt ctctttttga gttgaaacga agaaaaagtg ttagtagaaa atcgccttcg
       61 attttccacc aacacaaaat attatagaag caaaatcaat tctaccttag atgttcttat
      121 atcactctat cagtcctatt tgacaaagag ccaagaaaaa gacgttggtc gtcgatgctg
      181 ttctctcgcc tactcctcgt gcttgtagaa gttgtggttc tactgtggta gatggaaacg
      241 ggaaagcaat tatagtgaaa aatgggaaga aggaaacgat tgtccgtttt gaacaataca
      301 atcatatgcc tttggttacg cgcctaaaaa agcaacgcta tacctgtaaa aactgtcgta
      361 cccattggac ggctcaaagt tattttgtcc gactcagcca ttcaatcgca gatcatgttc
      421 gatataaaat tgcttcttta ctgactgaaa aagtatcttt gtcttttatt gcgaaaagtt
      481 gtcaggtatc tttgactacc gttactcgta cattgaaaga gtttaaaagc tatttaccaa
      541 agccttctaa gaagattctc cccagagtat tgatggttga tgaatttcgt tcgcacgctt
      601 ccatagaaga taaaatgagc tttatttgcg cagatggtga aacaggaaac ttaattgctg
      661 tcttgcctac gcgtaaatta cctcgattaa caagctattt cttaggctgc accaatccag
      721 aagaagtaga attcttagtc acagacatga acgccgctta cttccagctt accaaacgtg
      781 ttctgccaaa tgcgaaagta gtgattgatc ggtttcatat tgtcaaacac atgaatcagg
      841 cactcaatga gttgcgtatc cgtgaaatga atgagcttcg taaagcagga cagaaaagtc
      901 aggcagaaaa actgaaaaag aactggcgct ttctgctaaa aaatcgtgca agcatcaacc
      961 attatgagta caaaacatgg aaaagtttcc gagcaccaaa atacccattt ctcactgaag
     1021 caatgatgat tgatcgattg cttgagtttt ctgcgcctct aaaggaggcg tatccctttt
     1081 ttcatgaatt agtcgaagcc tttcgagaca aagaccctga cttatttttc tccttattgg
     1141 cagaacttcc cgaaacgtta gatgacggct ttcgggagaa gcttcaaaac cttctgacct
     1201 atgaagaagg tatcaccagc gcaatgatct atccttattc caatggaaaa attgaagcga
     1261 agaataccca cataaagaca atgaaacgag tatcctacgg atttaaatca ttcgagaaca
     1321 tgagaattag aatctttttg atcaatcaat taatcaatgt aagataacaa aaaatctgaa
     1381 ccagaatgtg gttcattctg acccggattt gattttgcac aactcatcag tccttattga
     1441 caaagagcca aaaatctgag ccagaatgag gttcattctg acccagattt gattttgtac
     1501 aacccatcag tccttattga caaagagccc ttctttttag tattattata attcttccat
     1561 gcgttcttcc agtcgctgta aatcgcttgg acttaattcc ccaatttttt tccatttgac
     1621 ctttttgttg tttttgggta attcgtttag agttcctacg tcaatccaag attctttttt
     1681 tagtcctgcc tgtttccaat cccttatttc aacgtatttc agctttattt tatcggattt
     1741 agtatgatat tttgaagtaa ttgacagaaa cgaaattgtg tccagtccat tttttagaat
     1801 atagacaggc cggcgtttac cgccctctgc agctgtgaaa gcaacgtaga cggttgctat
     1861 atcattagta ttcatcttca ttgaaccatt cttcaatttt ttcgtcagtg tctagttttt
     1921 ggacaggcaa tttttccgct aatctttgaa tattatggat tcgtttactt ttttccgaca
     1981 attcaataga aaatgggatt tttccttgct caacaatttg cttataaaaa acggtgacgg
     2041 ctgttttggg gctcatgcct agttcatcaa taatgctttc tgcttcattt ttcaattttt
     2101 cgtccataga aatttgtaac ttagccattt tctccacctc tttctgacaa acacattata
     2161 tcacggaaag agttcaaatg gaattaatac aactactttt gaactccttg aaatgcagtg
     2221 tatttatcta aatataagga aaggatatag tgctaaatgg ccttgataga tagaaaagtt
     2281 tcaaaatccg ccacagcgat aattgaagcg aacactcaac gacacacaga acaggaaaag
     2341 actcctaaaa gaaccaaatt aactaggacg tgtctgaaaa ctccacaaag atgtggtatg
     2401 ctctggtaaa aaacgaatgg cacaaccacg aagaccgata tggttcttgg aaaactttct
     2461 ccaccctttt tgtaattgga gggatagtgg tttattgata gcgattttcc aagttcttca
     2521 agtggaacca gacattgagc accacggcta aaaagtgaat gaagtcttta tttttaatca
     2581 ataattttta atgattacca gtaattggtt ttttcatgcg ccctatttct atttgatgtg
     2641 gagttaaata atgctgataa gtaaaaattt ttccgtcaat taaactgcca ctagtgaatc
     2701 ctgttctatt ggtaaaaaga gaatagtctg ggtattcatc tacaccaaat gataaagatt
     2761 cagccataaa ctgtttttta ccagcaaatt cagcactgct accttgatgg atatgtccta
     2821 caaaaatacc taaaatatca ctttcggcaa tcaattcagc aaaaccttcc ggaacttcag
     2881 ttacaataga tggatcttcc cacgacaatg gatgatgaac caataaaatc gttccctttt
     2941 cagcaggtgt acccaaccac tcttgtaatt gaatcatctg tcctatcgat atttttcctt
     3001 cgtgctgggt atcttgagca gaatccaagg aaataattcg taacccattt tgattcgtac
     3061 aatggatata ctctcccgtt tcattgcttt caacactcat tccttcaaag aaaacactgc
     3121 gacgatcatg attcccacga caaaaatagt aaggtgtggt tgggaaatat tgatccaaca
     3181 ttttccgtaa gacttgataa tcagaaatcc ccccttcatg tactaaatca cctgttaata
     3241 gaataaaatc gtacactgtt aacgagagtt cttgtaacaa tttttccaat gccaactgtg
     3301 gatttttaat tgttggaaat agttcagcag taaacaagtt agtttgatag ttttctctaa
     3361 aatggatatc agagagatgc aaaaagcgca tggctattcc ctctttcttc aataaaataa
     3421 tcaggatatc ctaaattttc ttgcgtctga taatcaaaaa aatgcattct ttcaggttct
     3481 atgcggagat tgactgcatc accaatctgc caagtaggtt tttcattaag gacaatcatt
     3541 tctataccat cgactttaac ggtgtacgca taactttgac ctaatagctc acgatacttc
     3601 acttgaccgt gtaaatcgct tgtttctttt gaaaaggtta cgtgttctgg tcttatgcct
     3661 aaatacaatg agccactaac ctgaagaagt tgtttttgcc aagctggatt caatgttaaa
     3721 aattgactgg ctacctttaa tgttccttga tcataagtaa ctggcataat attcatgcct
     3781 ggcgaaccaa taaaatttgc agtaaaagta tttttaggtt tcgtataaac atttttcggc
     3841 gtatcaatca tctgaattct accttcattc aataaaacaa tccggtcagc taacgtcatc
     3901 gcttcaattt gatcatgagt gacgtagaca aaagtttgtt tataacgttg atgaattttt
     3961 accaattctt ttcgtgcatc caatcgtaaa cgcacatcca aatttgataa cggttcatct
     4021 aataaaaaat aattactccg tttaaccacc gcacgagcca atgctacacg ctgccgttgc
     4081 cctcctgaca aatctttagg aagtcgatct tggtatggcg ttaaacctaa catcttcaaa
     4141 gcttcttcca aacggtgctg aatgtcagct gtcttaattt tattcgccct taatgcaaaa
     4201 ataatattct cctttacagt catgtgagga tataaagcat aattttgaaa aaccattgcg
     4261 atctctcgtt ctccacaagg tagcttattt gctagttgtg tacctaaaaa aagctctcca
     4321 ctcgtaattt tttccaaacc agcaatcatt ctcagcaaag tagattttcc tgaaccagat
     4381 ggtcccagta acacaattcg ttctccagcg gccacagcta aatcaatttc ttttaaaatg
     4441 gtatgttctc catatttttt tccgatattt cttaattcaa gcgtttctcc catgatttgt
     4501 cctccaattt atttaatacc tgcagaaaca aaagtcccga taatatattt ttgaaaaatt
     4561 gcatacaaga ctaacggtac aaacattgta attaaactaa tcgccattgc tacggtaaaa
     4621 tttgtcccac cttcagaact aatataggtc tgcatcgcca acggcaaagt ataattatct
     4681 actgtccgta aaattaatac cggccaaaca tactcattcc aagaggtaat aaaaaaccaa
     4741 ataccggtag aagtaatcgc atgtctagta agaggaaaaa caattttact catgacggtt
     4801 gtttgtttga tgttatccaa tgccgccaca tctagaaggc tcactggaat ttcccgcatt
     4861 gtttgcgtca ataaaaaaat gcccatcgca tcagcaaatt gtggtattat cacaccccaa
     4921 gtagtatcaa ttaaattaag ttttgcaatc aacaaataat taggaatcat cgcaacagta
     4981 aaaggaatga acatcgtacc aatgatgata aagtaccaga ttttcttccc tttgattcgg
     5041 caataagtaa aactatatgc cgcaaaaaaa gctacgccta acttaacaac cgttataatt
     5101 gtagcaataa aaaaagtatt tctcgtaatt ttgataattg gaagattttc gaacaaatag
     5161 caaaaatttt ccaaggtaaa cggctgtggt atcaaactag taatcgtctg ataggcatct
     5221 ttcaatggct taaaagaatt ggccataata aacaataatg gatagataat aatgattatg
     5281 atgaacataa ataagagatg tggcaaccag cgtttaattt tcatagtaaa cccccttttc
     5341 cacaaattta aatattaccc ctaagaaaac agcaaatatt aataaagtta aaactgaaat
     5401 tgctgaagat tctccgactt tatacatttc aaaagccttt tgataagcat aatagatcac
     5461 gtttgcacta ccattattcg gtccaccacg agtgatgatc ttaatcggag taaacacata
     5521 ctgcagacct tgaacaatgg tcatcaccaa taaatatagt cctgtcgaac tactcatagg
     5581 caacacaatt gtaaaaaaaa tgcgccagat gggtacacga tccaacttag ctgcttcgat
     5641 catctcttga tcaattccca caattgctgc aaaaatgaca atgaaattat aaccaaaaac
     5701 tttccaagca gtaatacctg caatcacagc aattacccaa ccttgtaaat tcatccacat
     5761 aggcagtctt aaaccaaaat ttcccaacaa gacagcaatt ggtcccgata ccggatttaa
     5821 tatccatgta aaaaccatcc ccgccactac caaagaaatc acagctggaa taaaaaataa
     5881 tggtttatac aaattctgcc ctttttctat aactattcct aataaaaagg atgtaatata
     5941 tgggaacaca cagctcagca agacaaatag aacggtatag gacaaagtat tcttcaacac
     6001 tttatgaaag actggatcag tcagcacctt tacatagttg gcaaagccaa caaattcttt
     6061 agtgggggcc accatattcc agtcaaaaaa actcaaatag atcgtataaa tcaatggata
     6121 aaaaacaaac acgcccaaca aaataattgc tggcgccaga tacaaataac ctacatagcg
     6181 cttcatttcc gacagcctcc tcatccttat gtcaaaagca gacgaccttg tttttccttt
     6241 cttacaggcg ctgtcgtctg cttgctttgc ttatttgagt aactcattaa tttccttttg
     6301 agtagctttc atcgtttcat tgacggaatt acctgataaa attttatccc gcatatccaa
     6361 caatttttgt tcagcttcta atccagcatc tccaggaaat gaactccaac tgaccatctt
     6421 atccatctga tcaattgctg gtatcatcat ctgattttca gctaagaaag cttttaatcc
     6481 attttgggcg tctgcaacat ctttacgtgg tggtaaataa ccagtacctt ctgtccattt
     6541 agctaaagat tcaacactat aaagatattt taagaaatcc caagttgctt cctttttctt
     6601 atccgtttgt gatgtaacag ctaacattgc gccaccagca ggtagtttaa cttttttccc
     6661 ttcccaactt ggtgactcaa ctgctttagc agtaaaagta ctattttttt gaatgttgtc
     6721 cctttgagca atagtggtat agaccatcgc gacgttacca tcaataaagc tttgcacacc
     6781 ttgttcccac gtagtgtgta atgccgtctg gtcttttacc atcctttgat ataattcata
     6841 agcagcgata ccatctttag aagcaaaggt agctttgcca tcttttatta cacttgcccc
     6901 attactttct aaaattgctt gttgtgccca attatcagca ggttcttgaa tatacacgcc
     6961 atactttcca gttttttctt ttattatctt agaaaaagtt tgaacttctt cccaagtttt
     7021 tggaccattt tcatccaagc ccgctgcttt taatatatct tgatttaaat ataaaaccgg
     7081 attactaatg gagtaaggaa tacccacttg tttaccatct ttttcagaca gtgccatgat
     7141 attgtctaaa aaatgatctt taaaataatc agcattatcc ggatccactt cttctactaa
     7201 agattgagga ttagtatatt tgaaattttc tgcaaaataa tctttaaaag cccatcccac
     7261 ttgtactacg tccggtattt ctttggaggc aacagaagct tgtaaatttt gcatcaagcc
     7321 tttatacata tcaggattat attttgctac aaccttcact ttgtcttgag attgattgta
     7381 tttatctact aattcattca ctgtttttcc acctagggta tccgcattaa catgccaata
     7441 ctctatcgtt acacgaccct ccgatttttt tgttgtcttg gtattcccac taccacaagc
     7501 tgccaaagag atgacactta aaacagacac aacccctata acaagatttt tctttaacat
     7561 aacattaact tcctcctaat ttctttatgg ccatttttct aatcaagaaa tattatgaac
     7621 taccaatgta aatttctagt taattttctg taaataattc gaaatttgtg catttcgggg
     7681 tacagggtca ctggacatat ccatcaagct aagaataaag cccccatatg gtcatgtatc
     7741 ccatctccaa atctcttaac tcctctgttt tgattataaa atagtttatt ctgtgctcct
     7801 ttcttccata aataggccca tgcacaacgt gaggttatat tataaacacc acagcgcgca
     7861 aggtactcaa tcgtttctag ttcattgact gtccatacag aaaatgtcaa tcctagctgc
     7921 tgccccactt cacgggccaa cttagttaac catccttcat gaatattgac gatttgaacg
     7981 ctttgatagg ctaatttctg taaggtatcg atcaaatctg cttcggtaat atccccaagt
     8041 tttttccctt cgaactgatt ttcaatatta taaaatatat gctttcccca aatcgcagcc
     8101 caagataatt gtacatagcc actaagaaca atttgtggcc agatttgata ggtttgtgcc
     8161 agcgaaaaaa cggccatttc caatccctct tcttttaaat cacagttaat ggtcatggtt
     8221 gccttctcat tggccactgc ttgaaacacc tgctcaaggg ttaccgaatc tgggacaact
     8281 tcttacctgt catgggctag tattagcgta ccatctgttt gctgacgcac atctacttca
     8341 aaacaaagct ggcgatccaa acaaaagcga agaaatgccc agctattctc ctccgtattg
     8401 tcactaccag agtgtgcagt gatcatggtc attttgaccc ctcctttaat tccgtgattt
     8461 tctcccacac gtccactata ccgatgaagc gttaatttct tgttaatttc ttgtaaattc
     8521 cctgcgtttt ttcagtaaac tagttgtaaa tcttgcatgc tccttcctac gaactcatga
     8581 atcgtcgatt ttgcccttct agctagtttc accaattaag tagttctaca ttccccgctt
     8641 ttaacctact acaccttttt ttgttttcag ctattccttc ttcttgctga aagtccttga
     8701 ggttctttct tacaggatac catcaaggtg ttcaatggct tattcccatt gaatgaccga
     8761 gacttactat cagatttctg ctcgttgaac caagtctttg atacaggatt tcttccgtca
     8821 tcttctcaca aataccacct tgcgtttctc tagcgattgg tcgatgtgca ccctcccagt
     8881 ggactgtcac tacccctttt tgctatgcct agcccacaaa aaaagctgtc ctcacagaca
     8941 g
//
LOCUS       DAJSBL010000084         8920 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.086, whole genome shotgun sequence.
ACCESSION   DAJSBL010000084 DAJSBL010000000
VERSION     DAJSBL010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8920)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8920)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8920
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.086"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..777
                     /gene="menA"
                     /locus_tag="ONT85_002567"
     CDS             <1..777
                     /gene="menA"
                     /locus_tag="ONT85_002567"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HCR2379509.1"
                     /translation="NYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFVI
                     GVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVYV
                     NTFGSDVYDLTVNFSSGQFDLVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRDI
                     ENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKHL
                     DQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(1712..3058)
                     /gene="gor"
                     /locus_tag="ONT85_002568"
     CDS             complement(1712..3058)
                     /gene="gor"
                     /locus_tag="ONT85_002568"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HCR2379510.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(3192..4445)
                     /locus_tag="ONT85_002569"
     CDS             complement(3192..4445)
                     /locus_tag="ONT85_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HCR2379511.1"
                     /translation="MMKEMTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAY
                     GLRSRAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISA
                     VIVSDPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVA
                     EIRKNTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFG
                     QERNSLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNV
                     YKAAVDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVY
                     KFVGEVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRA
                     PNPMMILTMPVAQPVKPGDMIRKRK"
     gene            complement(4451..5374)
                     /locus_tag="ONT85_002570"
     CDS             complement(4451..5374)
                     /locus_tag="ONT85_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HCR2379512.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(5595..8087)
                     /locus_tag="ONT85_002571"
     CDS             complement(5595..8087)
                     /locus_tag="ONT85_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HCR2379513.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(8094..8561)
                     /locus_tag="ONT85_002572"
     CDS             complement(8094..8561)
                     /locus_tag="ONT85_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HCR2379514.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
     gene            complement(8697..8770)
                     /locus_tag="ONT85_002573"
     tRNA            complement(8697..8770)
                     /locus_tag="ONT85_002573"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8734..8736),aa:Arg,seq:acg)
     gene            complement(8828..8901)
                     /locus_tag="ONT85_002574"
     tRNA            complement(8828..8901)
                     /locus_tag="ONT85_002574"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8865..8867),aa:Pro,seq:tgg)
BASE COUNT         2479 a         1884 c         1485 g         3072 t
ORIGIN      
        1 aattacatgg attatgaaaa ggctcattct gaagtatata agtatgaaga aaatgtcatt
       61 ggccgcagaa atctatctcc tgtattgatt cgaaacatga ttttagctat gattgcattt
      121 gtttttgtta tcggcgttat tctaacgatc aatacgggtt ggcttttact tttgatggga
      181 tttatctgtt gttttattgg agtattctat acattcggac cgattccttt atctcgaatg
      241 cctttagggg aaattttcag tggggtgact atgggccttg gaatttttgc catggtcgtt
      301 tatgtcaata cattcggttc agatgtctat gatctgacag tcaacttttc atctggacaa
      361 tttgatttgg taggaaatct ttgggctata gcagcaatcg tattagcttc cttacccttg
      421 gtgtttacga ttgccaatat catgttggct aataatttgc gggatcttga ccgtgatatt
      481 gaaaatcatc gctatacact agttttttat atcggacgtc ctgcgggaac agtcttattc
      541 cagctattga tgtatgcttg ctatttcgtg attttaatgg ggttgttgag cggtatctat
      601 gaatggccga tcttggtcac cttcttgacc ttgccaattg tgaagaagca tttggatcaa
      661 ttcaagcaaa cgttacccca gcctatcagt ttcagctatt ccatcaaaaa tatggtctta
      721 ttcaatgcca gttatgtatt ggggctatta ctaacaatca ttatgcaagc catttaacag
      781 catgaagagg ttgtgaaaaa actgccctgc atcaagtaat aagaacagcc aaataaaaat
      841 agctccttat atttttcgtt tggttgggct tattaccgca gatgcaagct tttgaacacc
      901 gtttatcagg aggttgtgaa aaagtcgctt agtttcgagt attaagaaaa attttgtgaa
      961 aatggctttc cacatttttg cataaatttg gcttaatacc gagaaactgg cttttgaaca
     1021 ccgtttatca gaggttgtga aaaagtcgct tagtttcgag tattaagaaa aattttgtga
     1081 aaatggcttt ccacattttt gcataaattt ggcttaatac cgagaaactg gcttttgaac
     1141 accgtttatc agaggttgta aaatcagtgt tttgtcctga gtattagaga aacaaatgga
     1201 aaaatagttc ctcatatttt gccgtttttt gagctaatac cgaaggacag ctgattgaac
     1261 actatttgtt cggaaaaagt gactaactat gacacaattt ttttcacagt cccttttttc
     1321 acttgatttg attcgtacca gcgaacttga cgagaacttt cttggtttgg taactatatg
     1381 gtattctttt aacatcctcg tttgcttctt ctaatttatt tatacaaagt cacacttttt
     1441 taaacatctg ttcttcgata catttctatt tcttttaaac atcgatacca atattttgaa
     1501 cttaagagtg cataccaaat ggattcattt actcttcgtc agttacgaag ataatcaaaa
     1561 tctccaactt ccctacttat ttcgttttga gaattatcga agcaatctta cataaataac
     1621 taaaaaacaa aaaaccagaa aatcagtata ctgattttct ggttttacat ggtttgttca
     1681 ataaaaagct gttggaactg ttaatattaa attatcgcat cgttacgaat tcttccgctc
     1741 ctgttggatg gatggctact gtattgtcaa agtcttcttt agttgcaccc atcttgatag
     1801 ccacggcaaa accttgcagc atctcatcga caccaacacc aatagcatgt aagccgacga
     1861 ttctttcttc ttttccgaca cagatcaatt tcatttcgca tttttggcga taatcattta
     1921 atgcaaagta cattggggta aagcgtgaac gataaacttt caattcattt tcgccatatt
     1981 tttccaatgc ttcttcttct gtcaaaccaa ttgttgcaat tggtggatgc gtaaagacga
     2041 ctgtcggaac aagtttatag tcaagataag aatctttctt tccattaaat aaacgttctg
     2101 ataaacgtct accagctgcg attgcaacag gagtcagatc taatttaccg atcacatcac
     2161 caacagcaaa aatatgttca tccgttgtat tttggtattt atctactttg ataaaccctt
     2221 ttttgtccaa ttccacattt gtattttcca gacctaattt ttcggtattc ggtacacgtc
     2281 caccggcaaa aagtacacaa tcaccagtta aggtttcacc attttcaaat agcaccgtat
     2341 attcgtcatt gtttttagta atttctttag caatatgatg tgcgtaagtt tttattccag
     2401 cttcttggta catttggatc aaattgtccg aaagcatttt atcaaacgtt ctaagtggtc
     2461 gttcttttcg atatgcccac atgacttcac tacctaaacc acgaaataca cctgaaagtt
     2521 cagccgcaat gtatccagca cctaaaacga ttgctctctt aggtaactct gttaattcaa
     2581 aaaatccatt tgaatcaatt gcgtattctc caccaggaat ggtcatttta cttgcacggc
     2641 ctccagtagc gatcaagatt ttaggagcac gataggtttc accgtttact tcgatcgttt
     2701 gactatcgat aaaagtagcg taacctttta tagcctcgac atgattgcta tctaatcctc
     2761 tttgataagc accgtgtaga aaatcaatat atttttctcg tctttctact agaccggcga
     2821 aatcaacatt ttttaaatct gcttggatac cgtaacttga ggcatctcgt tcaattgttt
     2881 ccaaaatcgt acttgcttgc cacataactt ttttaggtac acaaccgacg ttgacacatg
     2941 ttccgcccaa ttcattcgct tcaatcagca aaacttttgc gccatgcatc cctgctctgt
     3001 ttgctgaagc aataccgcca gagccgccac caattacgat gtaatcatat atttgcattt
     3061 tgttcctcct tattgaagag atcaatcaaa acctttattt aacgatcctt tttctacaga
     3121 aaacaagtgt aacacttttc ttgaattttg tatgcgaaga tgcttaaaac agggatatat
     3181 cagctttttc cttatttacg ttttcggatc atatctccag gtttcaccgg ctgtgcgact
     3241 ggcatagtta aaatcatcat tggatttggt gctcggtcaa tggattcgcc ttcttcatta
     3301 tgcataactt caacggtttg actgaaatga ctgaatcctg gaccataaaa ttcgatttca
     3361 tctcctacag aaaacaagtt tctctgacgt atcgtagcaa tctgtgttgc ctcatcataa
     3421 gataatactt caccgacaaa tttatatact gggattttac gacgttcacc gaataattgt
     3481 tcgttttcag ttggtgtatt gtagtaaaaa cctgtagcta attctcgttg tgctactttc
     3541 cataactcgt cgatccattc ttgcttgcag acatagtttt caggatcttc catataactg
     3601 tctacagccg ctttatagac atttgacacc gtagatacat aatggatcga tttcattctt
     3661 ccttcgatct tgaagctgtc tacgccgttt tcaatcaatt ctggaatatg ttcgatcatt
     3721 gacatatcca ctgcactcat cgagaattct tcttccactt cgcctgtatc agttaaactg
     3781 tttcgttctt gaccaaaagg catatcgaac aatccatact tccagcgaca agattgagaa
     3841 catccgccac ggtttgcatc ccgcatcgac atatggttgg acaatgtgca tcgaccagaa
     3901 taagagatac acatggcacc atggataaat gcttcgattt cggtatccgt atttttacga
     3961 atctctgcca cttcctccat tgaaacttca cgagctaaaa cgacacgttc caacccttct
     4021 tctttccaga attccaatgt ctcaaaatta gttgctgaag cttgagtgga taaatggatc
     4081 ggcagacctg gggcttctgt cgcacaaatt tcgatcaaag ctggatcaga aacaatcact
     4141 gcagaaatac cgacatcacg caaactgcgg aaaaattccc ctgctccatc ttcatttcct
     4201 tcatgggtga ccatattagc agcaacgtat accttcgcat catgttcttt tgcaaaagca
     4261 actccttcag ccatttcttc ataagtaaaa ttacctgcac ggcttcttaa accatatgca
     4321 ttcccgccaa tatatactgc atctgctcca taatggatcg cggtttttaa cttttccagt
     4381 gttcctgctg gcgctaggat ttctggtcgt ttcaattttc tttttactgt catttctttc
     4441 atcatactcc ttatttgata gcagaaggat caatataaaa gaatcctgta tctaattcac
     4501 ggttttcagg atgcaattta ctgatttcgc tagtcatttt ttccgctttt tcttgatccc
     4561 agctaccttc taaaatttgt ttttttgctt cggcgaatag tcgagtgatg tttacgaatt
     4621 tttcgcctgg agtaaataat ccgtccaatt tccatgtaca gtaatgatgc tgataaagtt
     4681 cattcaattc attcgagaga ttcagatcat tgctggcaaa aatatgtgtg ccatgactat
     4741 cttcataaat agaataatgt gtctcttctt ttttaggttc cgagataaaa agacctcttt
     4801 cacgatcttt ttgttcgtct tgtttcgtat agttatagta attctgtagc agtggacgtt
     4861 tggattgatg gatgcatgta gcaccataca caagggtttc tacaggaata tcgagttttt
     4921 cttccatcgc cttcatctct tcaaaaggaa cttcacgagc aagtactgct ccaactgcgc
     4981 cttttttcgc ccagaaattg atttgacgtg cgctcgttac taatgtttca gcatcataaa
     5041 tatacggtat acgcaattca ggattttttt tcatcgtata aataatacct gtatcaccaa
     5101 ctgtgatcac atctacgttt atttctttta aaaaagccaa atattcaggt acatttttca
     5161 ttttttcagg atgaatgatc ccatttacag caatattcgc ttttttacca tacttgtgtg
     5221 ctaattctac caattgtcgc tgttcttctc gtgtaaatga agcaggcaga cgcaatccaa
     5281 attgctcttc accgaaatat agtgtatctg cacctgcagc aagcaactgt tctgcttgtt
     5341 caatagtttc tactgtggtt atgagttcga tcataatttt ctcctcctac aacttcataa
     5401 tagtagcata gctctaaaaa ctttccaata cttcttctat ttaaagtcgg atttttgaaa
     5461 attatttcac aaaaaatcaa aataaagttc gatatattct gtccaaatat aaaaagagat
     5521 tgtgacaaaa atcttgtcac aacctcttaa tcaagtctta ctattataaa aattcagcta
     5581 cacaggccaa gtaactatag ggaacctact gtttcctttt taggtccgcg aacattcaat
     5641 gtgatcttac cttttgaagc acctactgtt acgtggtcac ctaaacgaat ttctccgcca
     5701 agcaaagcct cgctcaatcg atcttcaatt tctttttgaa gtgcgcgacg gattggacgt
     5761 gcaccatatt caggatcaaa tccagctttc ccgatcacat cgattgcaga tggtgtgatt
     5821 ttcacttgaa tatcttgttc tttcaatcgt ttgataatcg ctttgctcat gatttggacg
     5881 attgtatgga tttcatcttt actcaatgaa tggaagacga cagtctcatc gattcgattc
     5941 aagaattcag gtcggaaagc tttctttaac tcttctaaga tacgtttttg catggctttg
     6001 tgatctttag taacatcttg gacattgaat ccgacatttt tttcttctcg aatttgtgtc
     6061 gctccgatat ttgaagtcat aatcatgatt gtatttctaa agtccacttt tctaccttta
     6121 gaatctgtta aatgaccatc atccaatact tgcagaagta aattgaaaac gtctggatgt
     6181 gctttctcca cttcatctaa gagaatcact gaatatggtt tttgacgaat tttttctgtc
     6241 aactgtccac cttcatcata ccctacgtat cctggaggag acccgattaa tcggcttgta
     6301 ctgtattttt ccataaattc tgacatatca acacggatca gggcatcttc acttccaaac
     6361 atgacttctg aaagtgcttt ggccaattct gtttttccga caccagttgg tccaaggaac
     6421 atgaatgaac caattggacg atcaggatct ttcaatccgc ttcgtgcacg gcggatcgca
     6481 cgtgaaactg ctttgacagc ctcatcttga ccaacgacac gttcatgcag aagtccttcc
     6541 agttctaaca agcgttcgct ttcttttttc tccaactgct gtagagggac accagtccac
     6601 tccgatacaa ctgtagcaac atcttcttct gttacacgat cagaataacc ttgctcctgt
     6661 tttgcttcaa ttgcagaaac tttttgcagc tttttacgta gtgctttttc ttgtctgcgt
     6721 aattgagcag cattttcaaa gtcttgtttt tggatcgctg cttccttttc ttgtactagt
     6781 tcatcgattt ctagtttgat cacagttgat tttgttaagt gatcggtttg atccaaacgg
     6841 actttcgctg ctgattcatc gatcaaatca attgctttat cgggtagttg acgagaagtg
     6901 atataacgaa cagaaagatt gactgctgct cgtacagctt catctgtgat ctccactcca
     6961 tggtgctctt catatcttgt acgtaatcct tgaagaatca cttctgcttc ttcaggagtt
     7021 ggctcatcta cttgaatgcg agcaaaacgt cgctctaatg cagaatcttt ttcgatatat
     7081 ttttgatatt catctagggt cgttgctccg atcgtttgta attctcctcg agctaacgcc
     7141 ggtttcaaga tattcgaagc atcaattgct ccttctgctc cacctgcacc aattaaggta
     7201 tgcaattcat caatgaataa gattacttga ccatcattat agatttcgtc gatgaccttt
     7261 ttcattcgat cttcaaattc accgcgatat ttcgtaccag caaccaaaga tcccatatca
     7321 agcatcatca ggcgcttttc ttgcatgtct tctggcactt cacctaaaat gattttttga
     7381 gctaaccctt cagcaatggc tgttttgcct acaccaggtt cacccaccag tacagggtta
     7441 ttctttgtcc gtcggctaag aatctggata agccgtttca cttctctcga acgaccaaca
     7501 actgggtcca gcttgttttc ttttgctaat ttcgtcaaat cacgtgctaa tgaatctagc
     7561 gtaggtgttc cttgcggtgt ttgccgttta tcttgtacat ttacatttct acgacgattc
     7621 attccttttc cagcagaatc accagctagc cccatttttt gtttcagaac ttgacgggct
     7681 tttgaaaggc tgatacctaa gttgatcatg attcgagtag ccatgatact ttcatctttt
     7741 aataagctca tcaaaatatg ttctgttcca actttttgag aaccagtttt cttcacttcg
     7801 ctgctagcta aagcaaaaac ttgtttcatt cttggtgaat atggaagaaa gactccttta
     7861 gggtaagaac ggataccacc ataaccaatc agatgttcaa tttcttcata aatttccgtt
     7921 tcattcaaat ccatttcacg taatactttt ccagcaattc cattttgctc gaccactaag
     7981 gctagtaaaa catgttctga actaactgat tgatgtttca accgttttgc ttcttcttgt
     8041 gcaatttcta atactgcttg ggctctttca gtaaaaagtt cttccatcta atctcacttc
     8101 ccatcttcat aacttaaacg ttccagaaat gctcggagaa tccgagcacg tgtatgatct
     8161 tcatattcat tgaaattcaa tgtgtttttt gcaattgcac tcaacatcaa gtttccttct
     8221 ttttttgtaa ttatttggtc ttcataaagt ttttgaataa tagcaaaagc atttttctca
     8281 ctgattgtat catcaaataa ctgatttatc tgctcaagta attgtttttt atctgaaatc
     8341 cttacttttg caattcgtat ataaccacca ccaccacgtt tgctttctac tagataacca
     8401 cgttggatgg taaaacgggt attaatcaca tagttgattt gtgaaggaac acagttaaag
     8461 aggtctgcca tttcaatcct tcgaatctcg atcatttcac tattttccag aattttcttt
     8521 atatatgatt caattaaatc cgaggtattc tgatgtgcca tcttattcat tccctttgac
     8581 taatattgac cttaattata acgaatttga taatagaaag aaagaagaac gctttttgtt
     8641 aaataaatcc tttagaatca acaaaaaaaa atacggaata tactattccg tatcgacgcg
     8701 cccaagagga gtcgaacccc taaccttttg atccgtagtc aaacactcta tccagttgag
     8761 ctatgggcgc atattcaatt taaaaaaatt ataaatcatt tgaaaacaaa aagcaagata
     8821 aaaaaaatcg ggaagacagg attcgaacct gcgacccctt ggtcccaaac caagtgctct
     8881 accaagctga gctacttccc gtttaaaaaa taaaaaagac
//
LOCUS       DAJSBL010000085         8691 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.087, whole genome shotgun sequence.
ACCESSION   DAJSBL010000085 DAJSBL010000000
VERSION     DAJSBL010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8691
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.087"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            123..515
                     /locus_tag="ONT85_002575"
     CDS             123..515
                     /locus_tag="ONT85_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2379515.1"
                     /translation="MLDDRLTCSNCGTLFYYEEVRNIVKHRKKEMPIRCPNCNEVIKR
                     KLSHGYFVSYELYEESKINLKKKPKYYKNLKTGAIITEKEFKNLVTEEESRFYNLSGN
                     ICPGFMDQIIENESIESIENYVPVDLPK"
     gene            complement(688..870)
                     /locus_tag="ONT85_002576"
                     /pseudo
     CDS             complement(688..870)
                     /locus_tag="ONT85_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            857..1054
                     /locus_tag="ONT85_002577"
     CDS             857..1054
                     /locus_tag="ONT85_002577"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2379516.1"
                     /translation="MKDSIVEPEGMENTFELYNKTTVDKLIPILVPTDLNGHDVLLRF
                     NGFLGEDYRYLKISDPQPLTE"
     gene            complement(1097..1672)
                     /locus_tag="ONT85_002578"
     CDS             complement(1097..1672)
                     /locus_tag="ONT85_002578"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HCR2379517.1"
                     /translation="MAQLFYRYGTMNSGKSIELLKVAHNYEEQGKRVIIMTSAIDTRS
                     GFGKVESRIGVSKPAIAITSEMDVYGELVEIIDQYGKVDCILVDESQFLSRENIIDLS
                     LIVMNEEVPVICYGLKNDFQNNLFKGSEALLTFADKIEEIKTICHFCNKKAVMNARIV
                     DGIPVYSGEQIVVGGNEDYLSVCRNHYYNED"
     gene            complement(1827..2353)
                     /locus_tag="ONT85_002579"
                     /pseudo
     CDS             complement(1827..2353)
                     /locus_tag="ONT85_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            2810..4321
                     /gene="gadC"
                     /locus_tag="ONT85_002580"
     CDS             2810..4321
                     /gene="gadC"
                     /locus_tag="ONT85_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015082498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate:gamma-aminobutyrate antiporter"
                     /protein_id="HCR2379518.1"
                     /translation="MNKKKLSLFGFFALTASMVLTVYEYPTFATSQLHLVFFLLLGGI
                     LWFLPVALCAAEMATVEGWKDGGIFSWVSQTLGERFGFAAIFFQWFQITVGFITMIYF
                     ILGALSYVINFPELNTDPFIKFIGVLVIFWVLTFSQLGGTNRTAKIAKAGFVIGIVIP
                     SIILFGLAAAYFIGGNPIQMAINAKAFIPDFSQLSTLVVFVSFILAYMGVEASASHIN
                     ELENPQRNYPLAMFLLVILAIALDAIGGFSVAAVIPQHDLSLSAGVIQTFETLILHFN
                     SHLHWLVKVIALMIACGVMGEVSSWVVGPSRGMFAAAQRGLLPVFFRKTNKQDVPVPL
                     IMIQGIIVTIWAAVLTFGGGGNNLSFLVAISLTVVIYLVGYLLFFLGYFVLVFKKKDL
                     KRTYNVPGKTVGKTIIGGIGFILSVFALLISFVPPASIAASEHRTYQIILLISFVVTV
                     LLPFIIYEVHDKSAHTTIEEPTHVKAEDVNPAVYPAARGEHHVTKNDEHILKH"
     gene            4334..5734
                     /locus_tag="ONT85_002581"
     CDS             4334..5734
                     /locus_tag="ONT85_002581"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCR2379519.1"
                     /translation="MLYGKDNQEEKNYLEPIFGSASEDVDLPKYKLNKESIEPRIAYQ
                     LVQDEMLDEGNARLNLATFCQTYMEPEAVKLMTQTLEKNAIDKSEYPRTTEIENRCVN
                     MIADLWHAPNNEKFMGTSTIGSSEACMLGGMAMKFAWRKRAEKLGLDIQAKKPNLVIS
                     SGYQVCWEKFCVYWDVELREVPMDEKHMSINLDTVMDYVDEYTIGIVGIMGITYTGRY
                     DDIKGLNDLVEAHNKQTDYKVYIHVDAASGGFYAPFTEPDLVWDFQLKNVISINSSGH
                     KYGLVYPGVGWVLWRDQQYLPEELVFKVSYLGGEMPTMAINFSHSAAQLIGQYYNFVR
                     YGFDGYRDIHQRTHDVAVYLAKEIEKTGIFEIINDGSELPVVCYKLKEDPNREWTLYD
                     LSDRLLMKGWQVPAYPLPKDLDQLIIQRLVVRADFGMNMAGDYVQDMNQAIEELNKAH
                     IVYHKKQDVKTYGFTH"
     gene            complement(5899..7339)
                     /locus_tag="ONT85_002582"
                     /pseudo
     CDS             complement(5899..7339)
                     /locus_tag="ONT85_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328084.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
     gene            7732..8325
                     /locus_tag="ONT85_002583"
                     /pseudo
     CDS             7732..8325
                     /locus_tag="ONT85_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328085.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ParB-like nuclease domain-containing protein"
     gene            complement(8309..>8691)
                     /locus_tag="ONT85_002584"
     CDS             complement(8309..>8691)
                     /locus_tag="ONT85_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCR2379520.1"
                     /translation="FSRIKQPLTEVEIFHTDRGKEFDNQAIDELLTTFDINRSLSHKG
                     CPFDNAVAESTYKSLKVEFVYQYTFETLQQLDLELFDYVNWWNHLRLHGTLGYETPVG
                     YRNQRLAQRILDNELGCANASEAV"
BASE COUNT         2759 a         1447 c         1567 g         2918 t
ORIGIN      
        1 attttttctt ttaagtaaaa aatataggtg attttttttg acttcatatt tataataaag
       61 acacgtaaat caattatttt tcattttact ttaactaata atgtttattt ggagggatta
      121 ttatgttaga tgatagattg acgtgttcta attgtggtac attattttac tatgaggaag
      181 ttagaaatat tgtaaaacat agaaaaaaag aaatgcctat acgttgtcca aattgcaatg
      241 aagttattaa gcgaaagttg tctcatggtt attttgtcag ctacgaacta tatgaagaaa
      301 gtaaaataaa tctgaaaaaa aaaccaaaat actataaaaa tttgaaaact ggagctatta
      361 taactgaaaa agaatttaaa aatttagtga ctgaagaaga aagcaggttt tataatttgt
      421 ctggtaatat ttgccctgga tttatggatc agattattga aaatgagtca attgaatcaa
      481 ttgaaaacta tgttcctgtt gacttaccaa aatagaaaaa ataatcctgt ttttacagtg
      541 aattatttta attttgattt tcaataaaaa tatattacaa taataatcgt aatagctaat
      601 gaaacaattt ctcaatcttt ttaccactcc aaacagggag cgtcaataat tttgtgtaaa
      661 taactcgtcc ttctgcaaaa taatgggtta ctcagtaaac attgaagcta atgtatccgt
      721 cacttgttgg aagcctttat ggcttctatt taagaatttt tgattgtatg tatcaaagat
      781 ggatactagg aaacgctcta gtgattcttc attttgaaac tgctcttttc tgcggctgta
      841 tttcttgatt tgtttattga aagattcgat tgtagaacca gaaggaatgg agaacacttt
      901 tgaactttac aacaaaacta ctgttgataa attaattcca attttagttc caactgattt
      961 aaatggtcat gatgttttac ttcgttttaa cggattttta ggtgaggatt atagatacct
     1021 aaaaatttct gatcctcaac ctttaacaga ataataaaaa acgtgtaatc aattatttga
     1081 ttacacgttt tttatttcaa tcttcgttgt agtagtggtt gcgacatacc gaaaggtaat
     1141 cttcattacc tcctactaca atctgttcac ctgaataaac tggaatacca tcaacaatac
     1201 gagcattcat tacagccttt ttattgcaaa aatggcaaat agttttaatt tcctcaattt
     1261 tatcagcaaa tgtcaataat gcttcggatc ccttgaagag attattttga aaatcattct
     1321 tcaatccata gcaaattact ggtacttcct cattcattac gataagtgat agatcaataa
     1381 tattctctct acttaaaaat tgagactcat cgaccaaaat acaatcaact tttccgtact
     1441 gatcaataat ctccactaat tctccgtaca catccatttc agaagtaatt gcaatagctg
     1501 gtttcgaaac acctatacga ctctcaactt ttccaaatcc tgatcgagta tctattgcgc
     1561 ttgtcattat gataactctc ttcccttgtt cttcataatt atgagccact ttcagtagct
     1621 caatactctt tccactattc attgttccat atctgtaaaa taattgtgcc attaaattta
     1681 ttcccttctt ttacaattca attattcctt aattccatct gcttttatgc agctgctcgt
     1741 ttattccttg atatgtctat aacatctact atccctaaaa ccaatttata agccattaga
     1801 gatggtattc ctgaacatct ctcatattgt tttttgagtg gtatatcatc tgaaaaactg
     1861 aaggaatcat gaaaatagcg acatacttat catttagagt tctaacttta aatgcattta
     1921 cttcttcagt cagcacttta gtcatgttgg acatggtttg gggcgtgtag tgatgcccat
     1981 acattttctc aatcaagtct gcgatttccg acatggtaat accttttcgg aagaggtgaa
     2041 tgacggtctc ctctaatgtg tcattcgtcc gtctataagc aggaacagtc tgttgcttga
     2101 actcgccatt gcggtcgcgc ggaatctgga gatgaagttc cccatactcg gtcttgaccg
     2161 tacggtcata ggagccgtta cgagaattac ccgtgttaaa accaatgcga tcgtactttt
     2221 cgtaatcgag gaaagccgtt aactccgttt tgagaagcgt attgacagct ttttccaaat
     2281 ggacacggaa taatccattc aaatcgcctt tgttcgctag agtctttagg atttctgtag
     2341 taaaatcgtt catatggaag tcctcttttc tgtgaatgtg ttgtggtaac tctattctac
     2401 agaagggact tccttttttc tatttacaca aaatatttta cactctcaac aaaagtatct
     2461 tcgattaata cttgctcaag ttatgtgcag ctacgaaaaa gtaggtaccg aggtactttc
     2521 tttttcgcag ctttttttgg acttatgaga agtacattcg ctggaagaat acgatataaa
     2581 acgagctttt tcactaaaga tattaataga gtgaaagaaa caagcttttg ttgtcagttt
     2641 tttcactttt ctaaaaatga aaataaaaaa ttctggaagt ggagaagaat tttgcttgct
     2701 atactttgtc ttgtagaaaa gaggtggaca aaataatgca agctcaaata agcgattttc
     2761 cataaccttg gtaggaatga caaattaaat aggaaaggga agtaataata tgaataaaaa
     2821 aaagttatct ctattcggtt tttttgccct gacagcttcc atggttttga ctgtttatga
     2881 gtatccgacc tttgcaacat cacaattaca tttggttttc tttcttcttt tgggaggtat
     2941 tttatggttt ctaccagttg ctttatgtgc tgcagaaatg gcaacagttg aaggatggaa
     3001 agatggtggg attttcagtt gggttagtca aactttagga gaacgttttg gttttgcagc
     3061 tatttttttc caatggtttc aaattacagt aggttttatt acaatgatct acttcatact
     3121 aggagcgctg tcgtatgtaa ttaattttcc agagcttaat acagatccat ttatcaagtt
     3181 tattggtgta ttagtcattt tctgggtact gactttttca cagttgggcg ggaccaatcg
     3241 taccgctaaa attgctaaag caggatttgt cattggaatt gttattcctt cgatcattct
     3301 ctttggttta gcagcagcct actttatcgg cggtaatccg attcaaatgg ctattaatgc
     3361 gaaagctttt attcctgact tttctcagct ttcaacttta gtagtatttg tatcttttat
     3421 attggcttat atgggcgtag aagcttcagc atcacatatt aatgagctgg aaaatcccca
     3481 aagaaactac ccattggcaa tgtttttact agtgattttg gccatcgcct tagatgcaat
     3541 cggcggtttc tcagtagcag cggttattcc acaacatgac ttgtctctca gtgctggtgt
     3601 aatccaaaca tttgaaacgc ttattttaca ttttaacagt catttacatt ggttggtaaa
     3661 agtgattgct ctcatgattg cctgtggcgt tatgggtgaa gttagttctt gggtagttgg
     3721 tccatcacga gggatgtttg cagctgctca gcgaggtctg ttaccagttt tcttccggaa
     3781 aactaataaa caagacgttc cagttccgtt gattatgata caaggaatta tcgttactat
     3841 ttgggcagca gtcttaactt ttggtggagg aggcaacaat ttatcattct tagtagcaat
     3901 ctccttaact gttgtcattt atctcgtagg atatttacta tttttcctag gttactttgt
     3961 attagttttt aagaaaaaag acttaaaacg aacttacaat gtaccaggaa aaactgttgg
     4021 caaaacgatt atcggtggaa ttggttttat tttatcagtg ttcgcattgc ttatctcttt
     4081 tgtaccacca gcttcaattg cagctagtga gcaccgaaca taccaaatca tactacttat
     4141 tagctttgta gtgactgttc ttttaccttt tatcatttat gaagtacatg ataaaagtgc
     4201 tcacacaact attgaggaac ctactcacgt gaaagctgaa gacgttaatc ccgctgttta
     4261 tccagctgct agaggagagc accacgttac aaaaaatgat gaacatattt taaaacatta
     4321 ggaggaattc attatgttat acggaaaaga taatcaagaa gaaaaaaact atttggaacc
     4381 aatttttggc tctgcaagtg aggatgttga cttgcctaaa tataagttaa acaaagaatc
     4441 cattgaacca cgaattgctt atcaattagt acaagacgag atgttggatg aaggaaatgc
     4501 gcgattaaac ttagctactt tttgtcaaac gtatatggaa cctgaagcag tgaaattgat
     4561 gacccaaacg ttagaaaaaa atgcaattga taaatcagaa tacccacgaa caacggaaat
     4621 tgaaaaccgc tgtgtaaata tgattgctga tttatggcat gctccaaata atgaaaaatt
     4681 catgggaact tcaacgatcg gctcttcaga agcctgcatg ctgggtggta tggccatgaa
     4741 atttgcttgg cgtaaacgtg ctgaaaaatt aggtcttgat attcaagcaa aaaaacctaa
     4801 cctggtgatc tcttctggtt accaagtttg ttgggaaaaa ttctgtgtat attgggatgt
     4861 ggaactgaga gaagtcccaa tggatgaaaa acatatgtca attaatctag atactgtcat
     4921 ggattatgtg gatgagtaca caattggtat tgtaggtatt atgggtatta cttacactgg
     4981 tcgttatgat gatatcaagg gtctgaatga tttagttgaa gctcacaata aacaaactga
     5041 ctataaagta tacattcatg ttgacgctgc atcgggtggc ttttatgcac catttactga
     5101 acctgatcta gtttgggatt ttcaattgaa aaatgttatc tcaattaatt cttcaggtca
     5161 caaatatggt ttggtatatc caggtgtggg ttgggtctta tggcgtgacc aacaatactt
     5221 accagaagaa ttagtattta aagttagtta cttaggtgga gaaatgccaa ctatggctat
     5281 caacttctct catagtgcag cacaactaat tgggcaatac tacaactttg tacgctatgg
     5341 ctttgatggt tatcgtgata ttcaccaaag aactcatgat gttgctgttt atttagccaa
     5401 agagatcgaa aaaactggta tttttgaaat cattaatgat ggatcagaat tgccagttgt
     5461 gtgctataag ctgaaagaag atcccaatcg cgaatggaca ctatatgatt tatctgatcg
     5521 tctgttaatg aagggatggc aagtcccagc ctacccatta cctaaagact tggatcaatt
     5581 aattattcaa cgcttagttg ttcgagcaga ctttggaatg aacatggctg gtgattatgt
     5641 acaagatatg aaccaagcaa ttgaagagtt gaataaagct catattgttt atcataaaaa
     5701 acaggatgtg aaaacgtacg gctttactca ctaaaattct tcagagaaat ataaaagaag
     5761 ttaattaact aataaaagga gttgtgatta ccacacagct ccttaaatct atttgaataa
     5821 atatatgata ttcatttgtt acactctcaa tcttagctgt tgttctattc atcattactc
     5881 ttattgtaag tagttcagtc aaatttgctt gttcaattga tcgataacag tatgaattgt
     5941 ttgaaaagta atcggatata taattgtttt ctcactacct aagccttctt ggtataaaga
     6001 actatcgcaa agtataatcc gctgagtaca aaagaaagtg tcgtctacta tttctctaag
     6061 ttgtttacaa atgatattac taatagaaaa gtgttctccc aaaatgtctt ctaactgttg
     6121 tcgatagtat aaatacctaa agtctgacgg agctactatg aacacttcaa ttggagattt
     6181 ccgtttatgc tcatttaaaa gacttaaact aatggttagt ttacgaatag aattctcatt
     6241 ccaacgcaga aataaattgt tttgttttcg ccattgttca agaatttcat gaacttctaa
     6301 gtacaaagcg cgttgctctt catttaacaa gtaagtctta tcaggttgaa atatctgaat
     6361 atcagcccaa agtgagcgga taaacgggag aagtgcttta tcaaaaagta tctgattttc
     6421 ttcagatata ttcagagata agataagttt ttgactgagc tcaatcacca aagggttatc
     6481 tgctacatgg ttttgataaa caacgttcaa gtctttttgt aacaattcaa agttatcgca
     6541 tagataattt ctagagttga atagaaagta gatataaagg cattcatctt cgtcatgaca
     6601 taaagagaaa gaaagttgct ctaatccttt ctgtattagc tgaaacaagg gagtatgtcg
     6661 ggcaactctt ttttcttcct gactaaaata tattgggtta gtcttagatc tctgcaaacc
     6721 gatagcgata cctaataaaa taaattgttt ttcaactgta gggtaacttc ttgaaaaaaa
     6781 atgtttttct acataatcaa tcaattgttg accgctttga ataattgatt tatttagctg
     6841 gatcaactct gtccgatttg tctcatagat tatcgttagt aacaaacttc gataatcctt
     6901 ttcaggaatt tcaaaatggc catcagcatt taagtactgc atttgtttaa aaaaattaat
     6961 caactcattt cgtaagttgt aaactttgct taacgaaatg aaggtagtat cagctatatt
     7021 actccaattg tgttcccctg ataaataatg tcctagtaca ttgaaaaagt cagagcgctg
     7081 atagatgagt tgagtgagtg ccaacctcga aaacgaagag tcgaatgcga ctgtaaagct
     7141 acttctagat ttctccactg aaataatttg ctgatggagt atgtcggtaa tgcattccaa
     7201 gtcgttagtg acagacagtg agtgggcaaa cgttcaattg atctgctatt tcgcgaatct
     7261 gaatggtccc tttttcgtgt aattgctcac aaatgtaaac ttgacgaaaa atacttttct
     7321 ccagataggt ttccaacata gtactcctcc tagtcatttt atacataact attataactc
     7381 taccaatcgg aatagtcctg taatatattg aatcaaaaat ataaattata aaataattag
     7441 atggggtatt tggtttaagt atcccttata tagtaataaa tttataggtg tgattctcga
     7501 attaataccc gaaaatgtga gagaattttt atggattttt taggatacaa attaattatt
     7561 tttgctttta tcctgattac cagtccagta ctgtcggtat cccattttgc agcaaaatca
     7621 tgttgataaa gtgacaagaa gactgatgag atatattatc caaattgaga agataattaa
     7681 tcggattctt gagtataccc tactttttaa gaaagtaggg tatacaagcg tatgaataaa
     7741 gacataactt tccctgtgat ggacgttcgg cttgttccaa ttaccaaaat acaggcgaac
     7801 gattataatc ccaatcgagt agcgtctccg gaaatgaact tattggagct ttctatcatt
     7861 gaagatggct ttactcaacc attagtttgt tattacaagg aaaaagaaga gaagtatatc
     7921 ttggttgatg tgcttcatag gtatcgtgtt gctaaggaaa gactcaactt aaaagttgta
     7981 ccggtaacag ttattgataa acccatataa gaacgtatgg cttcaacaat tcgacacaat
     8041 cgtgcaagag ggacccatga aataagagga atggaaattg tcatccaaag attagtaaaa
     8101 aatggatggt cagacaagag aatatccaaa gaattaggca tggacattga agaaattttt
     8161 cgctttaagc aagtgtctgg tggaatggca acagtttttt tgacaaattt tataaggtgc
     8221 agaacttctt tccgtatgct attccgattg tccttgacaa tgagcctcct cggatgtgac
     8281 gatcttcggc aggagctaac agttaaagtt agactgcctc gctagcgtta gcacatccga
     8341 gctcattatc aaggattcgc tgcgccaatc tctggttacg gtaaccaacc ggtgtctcgt
     8401 agccaagtgt accgtgcaac cgaaggtggt tccaccaatt tacatagtca aataactcca
     8461 aatccaattg ttgtaaggtt tcaaatgtgt attgatagac aaattctact ttcaacgact
     8521 tataagttga ttcagctacg gcattatcaa aaggacagcc tttatgactc aatgatcgat
     8581 tgatgtcaaa agttgttaat aattcatcaa tagcttggtt atcaaactct tttccacgat
     8641 cagtatgaaa aatctcaacc tctgtcagag gttgtttgat acggctaaat g
//
LOCUS       DAJSBL010000086         8495 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.088, whole genome shotgun sequence.
ACCESSION   DAJSBL010000086 DAJSBL010000000
VERSION     DAJSBL010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8495
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.088"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..174)
                     /locus_tag="ONT85_002585"
     CDS             complement(<1..174)
                     /locus_tag="ONT85_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006735101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR2379521.1"
                     /translation="MATKIADELENHVLETGMQKITTHASITAKPFFEKRGYKVINEQ
                     TVELRGQLFTNFLM"
     gene            complement(594..1715)
                     /locus_tag="ONT85_002586"
     CDS             complement(594..1715)
                     /locus_tag="ONT85_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HCR2379522.1"
                     /translation="MDKYNVKTSFQKWFSAINFSGLSENSQSLISDFDFYSKKLDFQT
                     TLKVLLHAVYEELPSYREIDRAFLDQRLCKELGIDSLCYSSLSRRAPEIKQEVLMEIF
                     TQLVARISAQQPSSKTTSLQLIDSTTIPLNKAWFPWAKFRKTKSGIKLHLNLCYLDKT
                     NQYPESFTMTNASEHDRNHLEVLVDKTQATYVVDRGYFDYKLLDKLNRDGYFFVTRTK
                     SNTKITILDQIEVADTTTRDGTIISDQQVILVGGVNHVTERFRLVTVLTKGQKILRMV
                     TNLFDVSPNEVADMYQARWQIELLFKHLKQNLTIKRLYSHSEQGAINQVILTLIATLL
                     TYVIKIELNTTATLFQLKRTFHYLMFEPAEEWFKRYRPG"
     gene            2043..3452
                     /gene="istA"
                     /locus_tag="ONT85_002587"
     CDS             2043..3452
                     /gene="istA"
                     /locus_tag="ONT85_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HCR2379523.1"
                     /translation="MDDIKTIKHLRNNRDQSIDQIRKAMNINWRTAKKYADSDQLFIT
                     DPTRKNKSMMDDGWGEIIGSWLEEDLRLRRKLRRTAKAIHEQLKPLGFPGSYRTVCVY
                     ISQWKKQRYDETNYQAVDRLDHPIGEAQVDFGTMEVMHEDHMKDIQLLIMSFPYSNAT
                     SASAMPAQNQECFLEALKQLFNQCGGVPKTIRIDNLTPAVKKTKSKFEEAQLTDEFLR
                     FTIHYGCDVQVCNPRSGHEKGHVENKVGYVRYNFFSTSPIMDSFESLNKQLEEQLKAD
                     RKRTHYSKHQLIEDLWKKENKALHSLPQEDYPIFKEVEAKLNRYHEMKLDQTVIHIPK
                     AYGYSQLTVVLTWNKFKVLTPHGEILMNEYRPYMNKKRLIPWSDIMKQWSNKLNVVSY
                     SRYWKYLPARLQNYLAIDNNHLKYRRIKQLLSLLATYDIQQINEQFYELVVQEDAPSD
                     FDVNWSNYDALTKTGGVQK"
     gene            3449..4177
                     /gene="istB"
                     /locus_tag="ONT85_002588"
     CDS             3449..4177
                     /gene="istB"
                     /locus_tag="ONT85_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HCR2379524.1"
                     /translation="MIGQTLEDYCKKLRLSHIITNIPRASFEAPEQFITDLFRLELET
                     RQQIKLERMEKLAKFPVPKSLKNYEWHEDIFLPSHLDKETLVSLDFLKRQENIVCVGS
                     PGTGKTHLAIALGRKACSEGVSTKFFRVNNLVEQLENALKKQQLASFKKRFETTELII
                     LDEMGYLPLTKEGAELLFQLISDWYEQKSIIITSNLEFSQWNRVFMDSRLTAALVDRL
                     IHHAHIISFKGESFRLKNSLSKSY"
     gene            complement(4305..5216)
                     /locus_tag="ONT85_002589"
     CDS             complement(4305..5216)
                     /locus_tag="ONT85_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HCR2379525.1"
                     /translation="MKIKIERSNKEKIPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GIYKNFTAIDQHTTHEKVVVKEKIVNTSGVENFTVDFVKEYFSWKNNKEVIEKRMTNL
                     EQYLTEEGLALSQDMVRADIPTSSEVVSVKILDVEKHSEEFVVSFVVDQKITEGKKTQ
                     SISSAYRLMIFEDEKGNHIVTSLPTMIGKPEKAKHKVRQVKSDSKIDSKTTEEITEFL
                     ETFFRLYPAASEKELEHYVEKDAMQPINTNLKFVELVNPIYKQKGKAIQVIVGVEYLD
                     ELSKTTSVFQYRLKLYKNENWTISNCE"
     gene            complement(5233..6240)
                     /locus_tag="ONT85_002590"
     CDS             complement(5233..6240)
                     /locus_tag="ONT85_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR2379526.1"
                     /translation="MNLKKLSIISLTFLTISFVGILMCVGLLFGEDGEGSGSSGTSPG
                     GSSVSEVVLKHRSMVEKYAKESDITEYVSILLAIIQVESGGTMEDVMQSSESAGLSPN
                     SLSTEASIKQVCLYFSSLVKCSKELGCDQDSIIQAYNYGDGYLDYVAKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENSGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGSSPGTGFDCSGLTQWCYAKVGLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLIFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTDSYWQQHIIGAGRYK
                     Q"
     gene            complement(6237..8366)
                     /locus_tag="ONT85_002591"
     CDS             complement(6237..8366)
                     /locus_tag="ONT85_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HCR2379527.1"
                     /translation="MKKKLFRIVGIVFISIVTLTVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAKSIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRTVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALSLGTKIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEGRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKRAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRTFAFGGGAAVGGKLATSSTKGKGSSKINRVSNRKNHSARSINELNSDLKK
                     QEQPVGTKKESRINLVGRKVGKVLDTKEIVKDKAKQAKNQVTDAPTNLKYSLHKGMEK
                     TKKVPEDFKRGPFEEKANRGELREKQRQRRDAKMDEKRKVLNEAWNRHEKGRENVPIK
                     KEIKPQKNELPENRSLKPRVQVHSNPEIKRKLSSQEVIPNGSTQPLSKNNSFQQVKQR
                     FTFPKRTNQQVQRKKNTRPKPKPGEKR"
     gene            complement(8371..>8495)
                     /locus_tag="ONT85_002592"
     CDS             complement(8371..>8495)
                     /locus_tag="ONT85_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCR2379528.1"
                     /translation="NGQCLMQDLYGRVGVVQVHPVFEELFHAFDTRPPVQERME"
BASE COUNT         2599 a         1729 c         1486 g         2681 t
ORIGIN      
        1 cattaaaaag ttagtaaata attgccctct aagttccacg gtctgttcat taataacttt
       61 atatcctcgt ttttcaaaaa atggctttgc ggtaatggaa gcgtgggtag tgattttttg
      121 cataccagtt tctaaaacat ggttttccaa ttcatctgca attttagtcg caataccttt
      181 tccttgatgg ctatgttttt gtcacaactt cagtattcct gtaacaatac ggatttcagt
      241 atacaacaac agcttttgaa aagatacttg caaaaggatt aagatatcta tgacctgcta
      301 tgctcactga tgttgaaaag aatgttttat taggattgag gtaagcaaga tagtgaatat
      361 gtccggaaag cctgtaaaat aaggcttttc ggacatatgt gaagttataa aaagatagcg
      421 aaaaatctat ttagtatcac taatgttgcg aacatatcgt cagaatagtc tgacatttat
      481 ttcttgctaa aaatttcaaa aaagtcaagt gctgacgcag cggatgacta ttttccagaa
      541 aattttagta tgtattcaaa aatcaaacgt gacaacagct attaactaca tcctcatcca
      601 ggtctgtaac gtttgaacca ctcttctgcg ggctcaaaca ttaagtaatg gaaggtacgt
      661 ttcaattgaa acagcgtcgc tgtcgtattc aactcaattt ttatcacata agtgagaagc
      721 gttgcgatta aggtgaggat gacttgattt atcgcccctt gttcactatg tgaatatagt
      781 cgtttaatgg tcagattttg ttttaagtgt ttgaacaaca attcaatctg ccaacgggct
      841 tgatacatgt cggccacttc gtttggcgat acatcaaaga gattggtcac cattcgcagg
      901 attttttgtc ccttggtaag aaccgttacc aacctaaaac gctctgtcac atggttgaca
      961 ccgccgacaa gaatcacttg ttggtcactg attatcgtcc catcacgggt tgttgtatct
     1021 gctacttcaa tttgatccaa gatggtaatt tttgtattag attttgtccg tgtaacgaag
     1081 aaatagccgt cacgatttag tttgtctaac aacttgtaat caaagtagcc gcgatccacc
     1141 acatacgttg cttgcgtttt atcaacaaga acttcaaggt gattacggtc atgctcactc
     1201 gcatttgtca tcgtaaaact ttcaggatac tgattggttt tatcaagata acagagattc
     1261 aaatgtagct tgatgccaga ctttgttttc cgaaacttgg cccatggaaa ccatgctttg
     1321 ttcaacggaa tagtcgttga atcaatcaat tgtagactgg ttgtttttga gcttggctgc
     1381 tgagcagaaa ttctggcgac taattgcgta aaaatttcca tcaatacttc ttgcttgatt
     1441 tctggcgctc tacgagataa cgagctgtaa cacagggaat caatgccaag ttccttgcag
     1501 aggcgctgat ctaaaaaggc gcgatcaatc tcgcgatagc ttggcaattc ttcataaacc
     1561 gcatgaagta acactttaag cgtcgtttgg aagtctagtt ttttactgta gaaatcaaag
     1621 tcgctaatta agctttgaga gttttctgat aaaccagaaa aatttattgc agaaaaccat
     1681 ttttgaaatg aggtttttac gttatactta tccatgcgtg agtccttttt attggtcttg
     1741 gataagtcat ccgagaatag tataaaggac ttttttgcgt ttggatagct ttgtattagg
     1801 cttttgatgg gttaaaaagt ctttgcaaca ctagtaattt agtatttatg tataaattga
     1861 aagatatttt accaccactt agtcttctta aaagtgatcc aacattttgt ttgtggcaga
     1921 atttaactac acactaatgc agccgaaagt acacaactat gcagcagttt gactgcgaac
     1981 tggtttcctt ccatcataag tttgaatgac ttagtgatgg aggtacagaa aggttgttaa
     2041 ctttggatga tattaaaaca ataaaacatt tgagaaataa tcgtgatcag tcaattgatc
     2101 agataagaaa agcaatgaat atcaattggc gaacagcgaa aaaatacgcc gatagtgatc
     2161 aactattcat tacagatccc actcgaaaaa acaaatccat gatggatgat ggttggggag
     2221 aaatcatcgg tagttggcta gaagaagacc tacgtttacg cagaaaactt cgtagaacgg
     2281 ccaaagctat ccatgaacaa ttgaaacctt tgggtttccc tggctcctat cgaacagttt
     2341 gtgtatacat ttctcaatgg aaaaagcagc gttacgacga aacgaattac caagctgtcg
     2401 atcgtcttga tcatccgatc ggagaagcac aggtcgattt tggaacaatg gaagttatgc
     2461 atgaagatca catgaaagat atacaacttc ttatcatgtc atttccctat agtaatgcga
     2521 cgtccgcaag tgccatgcca gctcaaaacc aagagtgttt cctggaagca ttaaaacaac
     2581 tatttaatca atgcggcggc gttccaaaaa cgatcagaat agataactta actccagcgg
     2641 tgaaaaaaac aaaatcaaaa tttgaagaag cacaactaac cgatgaattc ttacgtttca
     2701 ccattcatta tggttgtgat gttcaagtct gtaacccacg tagtgggcat gaaaaaggac
     2761 atgtcgaaaa taaagtgggt tatgtccgat acaatttctt ctcgacttcg ccaatcatgg
     2821 actcttttga gtccctaaat aagcaacttg aagaacaatt aaaagcagat cgaaaaagga
     2881 cgcattattc aaaacatcaa ttaatcgaag atttgtggaa gaaagaaaat aaagcgttac
     2941 attctcttcc acaagaggat tacccaattt ttaaagaagt ggaagctaaa ctaaatcgtt
     3001 atcacgaaat gaaattggat caaacggtta ttcatattcc taaagcatat ggctattctc
     3061 aattgactgt tgttttgact tggaataaat tcaaagttct tacgcctcat ggagaaatat
     3121 taatgaacga atatcgcccc tatatgaaca aaaaaagatt gatcccatgg agtgacatca
     3181 tgaaacaatg gagtaataaa ttgaatgtcg ttagttattc gaggtattgg aagtacttgc
     3241 ctgcacgcct tcaaaattat ctagcgattg ataataatca cctgaaatac agacggataa
     3301 aacaattact aagtttatta gcgacctacg atatccaaca aattaatgaa caattttacg
     3361 aattagtcgt tcaagaagac gccccctctg actttgatgt gaattggtca aactatgatg
     3421 cattaacaaa aacaggaggt gtgcaaaaat gattggacag acgttagaag attattgtaa
     3481 aaagcttcga ttaagccata tcatcacgaa cataccaaga gcttctttcg aagctccaga
     3541 acaatttatt accgatttat tccgtttaga actggagacc agacagcaga tcaaattaga
     3601 acggatggaa aaattagcca aatttcctgt accaaaatcg ctaaagaact atgaatggca
     3661 cgaagatatt tttctaccat ctcatctaga taaggagacc cttgtctctc ttgattttct
     3721 aaaaaggcaa gaaaacattg tttgtgttgg ctctcctggt acagggaaaa cacatctagc
     3781 gatcgcatta gggagaaaag cctgtagtga aggtgtttca accaaatttt tccgagttaa
     3841 caatctcgta gagcaattag agaatgcatt aaaaaaacaa cagctagcca gtttcaagaa
     3901 gcgattcgaa acaacagaat taattattct tgatgaaatg gggtacttac cattgacaaa
     3961 agaaggagct gaacttctct tccaactgat ttcagattgg tatgagcaaa agagcattat
     4021 tattacctct aatttagaat tcagtcaatg gaatcgagtg tttatggatt ctcgattaac
     4081 agctgctctc gttgatcggc taatccatca cgctcacatt atcagcttca aaggcgaaag
     4141 tttcagattg aaaaattcat tatctaaatc atattgaagg aaaaaactag ttgcagcaaa
     4201 actctgcact ttcggctgca tttttttgta aaaagctatt gcacaacaca aacatttgaa
     4261 cgacgtaatt ccaaatgttg gattgctttt agataatctt ttatttattc acaattgcta
     4321 atagtccaat tttcattttt gtatagtttt aatctatact gaaaaacact tgttgttttg
     4381 gacaactcat caagatactc aacaccaaca atcacttgaa tcgctttacc cttttgtttg
     4441 taaataggat tcactaattc cacaaatttc aaattagtat taattggttg catcgcgtct
     4501 ttttcaacat aatgctctag ttctttttct gaggctgctg gataaagcct gaaaaacgtc
     4561 tctaaaaact cggtgatttc ttccgttgtc tttgaatcaa tttttgaatc actttttact
     4621 tgtcttactt tatgttttgc cttttctggc ttaccaatca tagtgggtaa actcgttaca
     4681 atatgattgc ctttttcatc ttcaaagatc atcagacgat atgcagaaga aattgattgt
     4741 gtctttttcc cttctgtaat cttttgatct accacaaatg aaacgacaaa ttcttccgag
     4801 tgtttttcaa catctagaat ttttactgat acaacctcgg aactagtggg aatatccgct
     4861 cgaaccatat cttggctcaa agcgagccct tcttctgtaa ggtactgctc aagattagtc
     4921 attctctttt caattacctc cttgttattc ttccatgaaa aatattcttt cacaaaatct
     4981 acagtaaaat tttctactcc agaggtattc actattttct ctttcacgac cactttctca
     5041 tgagttgtat gctgatcaat ggccgtgaag tttttgtaga ttccaaaagc aaggctacaa
     5101 gacaataaaa gccaaagggc aagcaccatt tttcgatgct tgcccacgga tactactcgt
     5161 tctttcttct tttttggaat cttttccttg ttacttcgct caatttttat cttcattttc
     5221 cattcctctt ttctattgtt tataccgacc agcaccaata atgtgctgtt gccaatagct
     5281 atcggtaaga ttggtccaac ccaccggatc tcctgcatga aacattctgt tctctcctgc
     5341 ataaatgccc acatgagtca cataagtacc ggcatcataa gttgaatgaa agaaaatcaa
     5401 atcgcctggt ttggcatctt ttaagtcaat atgagtcata gcatcatatt gatcctgagc
     5461 cacccgaggg agtttcaaac caactttggc ataacaccat tgagttaaac cactacaatc
     5521 aaacccagtt ccgggacttg acccaccaaa tacataagcc gttccttcat acttgaacgc
     5581 ctcgtcaaaa attccttgga ctgttttatc atcaaactgt gttgtagtta ggtactgctt
     5641 cactaataag caataaaaca tattgccata gttataccgc cacccactat tttccttaat
     5701 cgcgatggga ttgggataat cgactttttg accacccgat ttttcttttg aaaaagattc
     5761 ggctaaagca aaactatgct tctttccgtt tttcgcgaca taatccaaat acccgtcacc
     5821 atagttataa gcctgaatga tactatcttg atcgcatcct agttctttgg aacacttaac
     5881 caaagacgaa aaatagagac acacttgttt gatggatgcc tcggtactta atgaattcgg
     5941 tgataaacct gctgattcag aactttgcat gacatcttcc attgtacctc cagattcgac
     6001 ttgaataatc gccaaaagaa tcgaaacata ctctgttata tctgattctt tggcatactt
     6061 ttccaccatt gaacggtgtt tcaaaactac ttctgagaca ctactacctc caggagaagt
     6121 tccagaacta ccacttccct caccgtcctc accaaacaac aaacccacac acatcaagat
     6181 gcccacaaaa gagatggtca ggaaggttaa cgaaataata ctcaattttt ttaagttcat
     6241 cttttctcac ctggctttgg ttttgggcga gtgttctttt ttctttgtac ttgttgattg
     6301 gttcgctttg gaaaagtgaa tctctgtttc acttgttgaa aggaattgtt cttgcttagt
     6361 ggctgagtgc ttccgtttgg aatgacttct tgactagaaa gtttccgttt gatttcagga
     6421 ttcgaatgta cttgtaccct tggttttaaa gacctatttt ctggtagttc atttttctgt
     6481 ggttttatct ccttttttat cggaacattt tcccttcctt tctcatgacg attccatgct
     6541 tcgttcaata ctttgcgttt ttcatccatt tttgcgtctc ttcgttgtcg ttgtttttct
     6601 cgcaactccc ctcgatttgc tttttcttcg aaaggccctc gcttaaagtc ctctggtact
     6661 tttttcgtct tttccatgcc tttatgaagg ctgtatttca agttagttgg tgcatcggtt
     6721 acttgatttt ttgcttgttt cgctttgtcc ttcacgattt cttttgtatc gagtactttt
     6781 ccaacttttc taccaactaa atttattcgt gattctttct tcgtgcccac tggttgttct
     6841 tgttttttca gatcagaatt tagctcgtta atgcttctcg cggaatggtt ctttctattg
     6901 gacactctat tgatttttga actacctttc ccttttgtag aactggtagc tagtttccca
     6961 ccaactgctg caccgccacc aaaagcaaaa gttcgaccca catttcgatt aagctgtcgc
     7021 aattttcgat tcatcaacat ttgcggtttc cgcatgactc gccttcccat tgactgacta
     7081 tcattacttt gaaggttaaa catgctcata atgtcaccta atttgaaata gataccggca
     7141 aaggtcacaa tttgtagaaa agctaccata aagaagggat aggtggccga aatattgaaa
     7201 aacatcgtgg agatactgaa tgcagtcgta atgactaagg taactcccgc tcgcatcata
     7261 atcgtattga acaaacgaat aatcgccctt ttgcccaaac tctcatacgt cggcaacatc
     7321 gacaataaga aactaatggg cagaaacatc gcaaagataa tgaaaaggat ttgtgagaac
     7381 aacataatcc ctgtcagaag aaacacaaaa aaggaaatcc caatattaaa caacactaaa
     7441 aagaccacgg ttcctaaacg gttcatggtc ttggtaatac tgagattcgc attgtcatta
     7501 tcttcaatct ctgctttgac tgcttcttcc cgatccttac ccttattttc atccggactc
     7561 acggataaaa tcttattaac ccgaccctcg ccaatttctt ctatattgga atcatcgaat
     7621 tgtaatagta accagggttg ttgaacttga atcgaaaata aactgtctcg aatcaaatcc
     7681 acactatctt ttccctggct ttctgaatta ggtacaacaa tcttggttcc aaggctaaga
     7741 gccgcttcac taacgtctga gctaaagtca ttgatttttg taatataggt gggcgcatac
     7801 gcaatgaaag aacccgagag tagaaaaatc atcacaaaat taatgacggt tcggactgct
     7861 ttggtagttt ctcgttttaa taatccggta tacgccacat aaactcctag agctaaaatc
     7921 atcaataata ggaacccaaa gtagaatccc gaagtctgta atccattttc agtgataccg
     7981 gctaaagttt ggatattctt tccaatactt ttagccgtat ccgaaatgaa atccaacttg
     8041 taagcttctt gaaccacata gcccgtggcg tttgataaat acagacttac tgtccaaata
     8101 aaattggtaa tggcataaag accatacatc acgcttttcc ctaagccatc cccccaattc
     8161 caaggtaacc aatcccaaga actatccaca aagaaatcca gttgataatt gttcagagaa
     8221 tatttggaat aaaggtttcc ggcttcaact gtatcatcaa ccagacctgt cgcctcagct
     8281 accgtaccga ctaaactcaa tagaactgtc agagtgacga tgctaataaa aacgattccc
     8341 acaatccgaa aaagtttctt tttcatcccc tcactccatt ctctcttgaa ccggcggacg
     8401 agtgtcaaaa gcatggaaga gttcttcaaa gactggatga acttgaacca cacctactcg
     8461 cccatacaag tcttgcatca agcattgacc gtttt
//
LOCUS       DAJSBL010000087         8328 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.089, whole genome shotgun sequence.
ACCESSION   DAJSBL010000087 DAJSBL010000000
VERSION     DAJSBL010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8328
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.089"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(61..1020)
                     /locus_tag="ONT85_002593"
     CDS             complement(61..1020)
                     /locus_tag="ONT85_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HCR2379529.1"
                     /translation="MTYTHLTTDELVIIESYFNIEKPVALVAQSLNRSRQTIYNVYSF
                     LKQGKKVLDYYRQYKQNKSRCGRRSTVLSDEQSTYVQEKVAQGWSPDVIIGRGEFPID
                     CSVRTLYRMFKKGLFDTQSLPMKGKRKPNNHQEKRGKQAFRRTIHERNSLYPHLTKEF
                     GHLEVDTIVGVKHKSAVITLVECLSKVIITLKPTGRRAEDIENRLNDWFKSMPKHLFK
                     SMTFDCGKEFSNWRSISNSNDIAIFFADPGTPSQRGLNEHSNGLLRRDGLPKTMDFRD
                     TDETFIQSVASKRNHIPRKSLNYRTPLEVFLSCIDKDILSSLI"
     gene            1116..1661
                     /locus_tag="ONT85_002594"
                     /pseudo
     CDS             1116..1661
                     /locus_tag="ONT85_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1844..4519
                     /locus_tag="ONT85_002595"
     CDS             1844..4519
                     /locus_tag="ONT85_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF927 domain-containing protein"
                     /protein_id="HCR2379530.1"
                     /translation="MKMNHTSIQKHLQLLNTLGISYVNLRTFSTLNKKTVSHIFSTAD
                     SQAIINYLEKQKLSEGTVNITYNPIKQEVITQKKHSVRDNDIEKIRFVFIDIDPKRKE
                     GSATDSEKKKAENVMEQVERYLKEKRIESFVKVDSGNGYHIFLPINEQPNNHETILTI
                     QNFLQLLHRRFGVEDEVDIDTSVYNPSRLCKLVGTPAVKGKSTKERPHRLSSFLSIPD
                     NIDRSNCFKILEQIVEENSMMSTSRVKSHPSFREVLPFIQADYKQWLAHYPELTYTIK
                     EGDHKGVTLLIFDECPLRKHTNNSNGSCLSVTADNKIRFHCLHASDKNKDIQDFVKKY
                     PLPKPVNPSVESLESNQEYYFDQFKLNQEGVFLVKEGQEEKVACPMFIKQIRKNIEQK
                     VITLVLAYYVDHCWDEIEIEGGCLQTNNFKQLAKHGLIIKARREAEVVDFLQIQRENA
                     PIFFEHESLGWKKENEAMIFQLQDSFGREIPSVLTKNSCYQLTTNGTEAEFDALVHKY
                     VLGTNLELAWSIGIASIVLGYLNVTKANTFLSLLINLQGKSTTGKTTTLHFIASLFGN
                     PTTVLRNMNATNKAIIKLASNNQGGVPLILDELGAATQNNFSSLIYQLASGEERLRLD
                     SNCQLMPQETFSVLTLTSSEERLENYFNEKLTGLKVRYIEFSDIKFTKDANHAEKVKS
                     LSSNTFGWGIKLFLLKLFEQGITKIEATFVEAQKKLWAVLPEHSLKQRIGNNLALIYS
                     GALLIQDILGYPLRLEHIQTVLINSYEQLIVDTSLGSEQTVFDRVKTLLVMNSHKFLT
                     NSDNKHTPSTIWGKVSIQKNGDIKVNIFPIPFEDLLRREFQVKDLTYLFRQLLDSGNM
                     CAEKGRRTKRIHMNRQVLPTYEILLPSSLKSYFRL"
     gene            4701..4940
                     /locus_tag="ONT85_002596"
     CDS             4701..4940
                     /locus_tag="ONT85_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2379531.1"
                     /translation="MTKNKMTLKAEVLLYIQEHFSNQAFFTKPIYLDFEIRGVSAGSI
                     GGTLQALKNEGYLENHFVQRSFNRRVVKKWYLVHS"
     gene            4956..6329
                     /locus_tag="ONT85_002597"
     CDS             4956..6329
                     /locus_tag="ONT85_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR2379532.1"
                     /translation="MRVACYDRTSVYEEAKISNDAQYDAVKEYVLEKYEQIPQRFSDK
                     GESGEKAERPAFRQLRAWVCASEEEGVIYVWRYDRLARQAKIALEFIELCQQHQVQVY
                     SISEPLPASFSHASTNKLFVQLLFTFAEQQRNVIIENVRAGLSYKKQQRQYLSSAVPY
                     GYRLVNGKIQQEVHEARIVRYIFQLYLTKKYGYKKLCQRLTQQKLFFRERPFQPYHIY
                     SILKNPLYYGEIKGGSLGKYLGTFEPILSKATFLQAQEIRQSRCTAKKDTYLYLLRQK
                     IRCPFCGRHLSSKYQWNTKKTKTLHYYHCTDRSCQGIFLRAERVESEVLEKIQAFFQY
                     EGVYQALIAQLTQQLAEKRKDEKRKVIQQKKQEAQWIKAFEEGNCSLEELKQQLQALQ
                     KPMSTSSFLSLQQCEAKVKEWLQLKDLSSRQLLCDYVCRIEVTKDKTVCGIYFRQLTQ
                     NILERNE"
     gene            6333..7967
                     /locus_tag="ONT85_002598"
     CDS             6333..7967
                     /locus_tag="ONT85_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR2379533.1"
                     /translation="MEMIQTKRVALYIRVSTQEQALEGHSIQAQEQNGRQFAERMGYE
                     VVEVYADEGISGKSTKNRFAYQRMMNDARNHRFDLLVIWKLTRLGRNMLDVLQTVEEL
                     LRYDVGLHSISEQFDVTTSTGKLMLQLLGSFGEFERNQIAENVQMSMQSLVKDKKRYA
                     GGRRLGYVSGIDTNGQKQLVIEPEEAKIVRLIYAKYLGGAGYRAIANFLNRQGYQTVK
                     KNAFSTTAVKDILRNKIYAGYLEYARYINWETKRRKGKNPRPILVKGTHEPIISEEDY
                     QKVQERLALEGKQPKWNHTGENVLTGLLRCPECGAPMAASNVTNTLKDGTKKRIRYYS
                     CSVFRNKGASVCHANSIRAEQAETFVADRLKEIVQLPEVLPRLVAALNEEIVRQSQPL
                     EQELVVLLERKEELKTKIEKWEAALEDSPELFPMLKDRLDELTEKRRQLHIRENEILG
                     IFQQGEPIQVKDVQRILTNLDRFLAQSEKKQIKALYRTFIEKITFDPNHKEMLEITMK
                     FTHAVVQQLNEQYQIAVSKTKDTAIFVLKTPFTLTI"
     gene            8130..>8328
                     /locus_tag="ONT85_002599"
     CDS             8130..>8328
                     /locus_tag="ONT85_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2379534.1"
                     /translation="MKKMGKCILCEKYTELTKEHVPPKKSGNTGGKKTRTGNLDDFLK
                     SDFTKGDFPKGIKRKPQGNVYY"
BASE COUNT         2874 a         1338 c         1644 g         2472 t
ORIGIN      
        1 tgaaaatcac ctttataaac tccattcttt tccactgttt tattaatatt ttctgatttg
       61 tcaaattaag ctagacaata tgtctttatc tatgcagctc aaaaatactt ccaatggtgt
      121 tcgataattt aatgattttc ttggaatatg gtttcttttc gaggcaacgg attgaataaa
      181 ggtttcatcc gtgtccctaa agtccattgt tttaggtaag ccatctcgtc taagcaagcc
      241 gtttgagtgc tcatttagcc ctctttgcga aggtgttcct ggatctgcga aaaagatggc
      301 aatatcgttc gagttactta tagatcgcca gttggaaaat tcttttccac aatcaaatgt
      361 catggattta aacaagtgtt taggcatgga tttgaaccaa tcatttaacc tgttttcgat
      421 atcttcggct cgtctgcctg tcggtttcaa agtaataata accttggata aacactcaac
      481 caatgtaata acagcgctct tgtgtttaac tcccacaatg gtgtcgactt cgagatgacc
      541 aaattcttta gtcaggtgtg gatagagact gttccgttca tgaatcgttc tgcgaaaggc
      601 ctgttttcca cgcttttctt gatgattatt cggtttacgt ttccccttca taggtaacga
      661 ctgtgtgtca aaaaggcctt ttttaaacat ccgatagagt gtacgaacag aacaatcaat
      721 tggaaactcg ccacggccga taatcacatc gggtgaccat ccttgagcta ccttttcttg
      781 aacatatgtt gattgttcat cagataaaac ggttgaacgt cgtccacaac gagatttatt
      841 ttgtttgtat tgacggtaat aatctagcac tttttttcct tgtttgagaa acgaataaac
      901 attataaatg gtttgtctag agcgatttaa actttgtgcg acaagagcga caggtttctc
      961 gatattgaaa taagactcta tgattactag ttcgtctgtt gtaagatggg tgtaagtcat
     1021 ttgtactcac tccttattaa ttctttggtc ggaactattt tgagtgtagc acaaatgatc
     1081 tttttagttg tctagcttaa ttttacaatc gacgttttca aaatgctgag actattgaac
     1141 gagaacatca ttatttcgat ttattagtga atacagtggt agaattagcg aaatgggcaa
     1201 agtcaaacga aacttatagt caagtactta aaagaattat gagtcaagaa acttcattta
     1261 tctatgagtt aagcaagtca aatttgaaat atacttataa tgaagatgaa cttgaaattt
     1321 tagataatcc agaaaattca caatggatta aagagttgaa gagagaagac tttattgaga
     1381 cactaagaat tctaaaatcg ttatttgatt tgaaattagc caaagctacc atcatgggaa
     1441 ttgaaagaga ttcatattat tgggaggaaa ttggactata tgctccccag attgaaggtg
     1501 aagataaaga gaaaaggata gacaacatta ttaactatat taatagttta agtgaacctt
     1561 taaaatgtag aggacttgat tttataggaa cattaggatg gacagaaaca tactatgaga
     1621 aaatatataa agaaataatt gaacaatatt ttcctaaata atttaataag gattgattta
     1681 tcgtaaaaga aaaaagttgt gatttccaaa atcacaactt ttttcttttg tcaagatttt
     1741 gagtgggata tatttttgta gaactgaaga tcatgaaatt atgagaggtt ggaggtaatg
     1801 tagtcataca aataaaaatt ttttagaaag tgagtggtaa ttaatgaaaa tgaatcacac
     1861 ttcaattcag aagcatttac aactgttaaa tacactgggg atttcttatg ttaatttgag
     1921 aactttttct acattaaaca agaaaaccgt ttctcatatt ttttctactg cagattctca
     1981 agcaattatt aactatttag aaaaacagaa attgtctgaa ggtacagtaa acataactta
     2041 taatcctatt aaacaagagg tgattacaca aaaaaagcat tctgtgagag acaatgatat
     2101 tgaaaaaatt cggtttgtat ttattgatat tgaccctaaa aggaaagagg gaagtgcaac
     2161 agatagcgaa aagaaaaagg cagaaaatgt tatggaacaa gtagaacgtt atttgaaaga
     2221 aaagagaata gaatcatttg taaaagttga tagtgggaat ggctaccata tttttcttcc
     2281 cataaatgaa caaccaaata atcacgagac gattcttact attcagaatt ttcttcaatt
     2341 acttcaccga agatttggag tagaagatga agttgatatt gatacttctg tttataatcc
     2401 ttcaagatta tgcaaattag ttggtacacc agcggtcaaa ggaaagtcca caaaagagcg
     2461 tccgcatcgt ttatctagtt tcctttctat tcccgataac atagatagaa gtaattgttt
     2521 taaaattctt gaacagatag ttgaagaaaa ttccatgatg tcaacctcta gagtaaaaag
     2581 ccatccatct tttagagaag tgttaccttt tattcaagca gactataaac aatggttagc
     2641 tcactatcct gaattaactt ataccataaa agagggcgat cataaaggag ttaccttact
     2701 tatatttgac gaatgtccat taagaaaaca cacgaataat tctaatggtt cttgtttgtc
     2761 tgttacggct gataataaga ttcgatttca ttgtctgcat gcttcggata aaaataaaga
     2821 tattcaagac tttgtaaaaa aatatccgtt gccaaaacct gtaaatcctt ctgtagaatc
     2881 attagaatcc aatcaagaat attattttga ccagtttaaa ctgaatcaag aaggagtatt
     2941 tttggtgaaa gaaggtcaag aagaaaaagt tgcttgtccg atgtttatta agcaaatacg
     3001 caaaaatatc gaacaaaaag tcattacact tgttcttgcc tattatgtcg atcattgttg
     3061 ggatgaaata gaaatagaag gtggatgttt acaaactaat aattttaaac aattagcaaa
     3121 acatggctta atcattaaag ctagaagaga agctgaagtc gttgactttt tacagattca
     3181 acgggagaat gctccaatct tttttgaaca tgaatctctt ggctggaaaa aagaaaatga
     3241 agcaatgatt tttcagttac aagattcttt tggacgtgaa attccttctg tattaactaa
     3301 aaactcatgc taccaattaa caactaatgg tacagaagcg gaatttgacg cattggttca
     3361 taagtatgtt ttaggaacga acttagaact tgcttggtcg attggtattg cttctattgt
     3421 gttaggctat ttaaacgtta cgaaggctaa tacttttttg agtttactaa ttaacctaca
     3481 gggcaagagc acaacgggta aaaccactac gttacacttt attgcctctt tatttggaaa
     3541 tccaactacg gttcttagaa atatgaatgc taccaataaa gccattataa agctagcatc
     3601 aaacaatcag ggaggtgttc cactaatact agatgagtta ggagcagcaa ctcaaaataa
     3661 ctttagctcg ttgatctatc aattagcttc aggagaagaa aggctaagat tagattctaa
     3721 ttgtcagctt atgccacaag agactttttc tgttttgacc cttacctctt ctgaagagcg
     3781 tttggaaaat tatttcaatg agaaattaac tggtttgaaa gttcgatata tcgaattttc
     3841 tgatatcaag tttacaaagg atgcgaatca cgcagagaaa gttaaaagtc tttcttctaa
     3901 tacttttggt tggggtataa agttatttct tttgaaactc tttgaacaag ggattacaaa
     3961 aattgaagca acttttgtcg aagctcaaaa aaaactatgg gctgttttac ctgaacattc
     4021 tttgaagcaa cgcattggta ataatctagc tttgatttat agtggagccc tcctaattca
     4081 agatatactg ggctatcctt tacgattaga gcatatacag acggtgctta ttaattcgta
     4141 cgaacagtta attgtagata ctagtttagg aagtgagcaa acggtatttg accgagtgaa
     4201 aacattgttg gtgatgaata gccataaatt tttaacaaac agtgacaaca aacatacgcc
     4261 cagtacgatt tggggaaaag tatcaattca aaaaaatggg gatattaagg taaatatttt
     4321 tcctatcccg tttgaggact tgttaaggcg agaatttcaa gtaaaggatc taacctatct
     4381 ttttaggcaa ttgcttgatt cgggtaatat gtgtgcagaa aaagggagaa gaacgaaacg
     4441 tattcacatg aatcgtcaag ttcttccaac atacgagatt ctattacctt cttcactcaa
     4501 atcgtatttt cgattgtaat ttagaataaa ccaaagtatg aaagtgaggc accatgttat
     4561 ggaaatattt cttattgatt tagcaaaaca actgattact tatctgtatg atagcgagca
     4621 aacccaaacg gaagtaaatg aagcttgatc tatgcttcat ttacttcatt caatcaaaca
     4681 tgaaagaaag gaaaattcta atgactaaaa ataaaatgac actaaaagca gaggttcttc
     4741 tgtatattca agaacatttt tcaaatcaag ccttttttac aaaaccaatt tacttggatt
     4801 tcgaaataag aggggtatct gctggatcta tcgggggtac tcttcaagcg ttgaagaacg
     4861 aaggatattt agaaaatcac tttgtgcaac gttcgttcaa tagaagagtc gtgaaaaaat
     4921 ggtatttagt tcattcatag agaggggaat caagcatgcg tgtggcttgt tatgatcgaa
     4981 cgtctgttta tgaagaagcg aaaattagta atgacgctca atatgatgca gtgaaagagt
     5041 atgttttaga gaaatatgaa caaattcccc aacgattttc agacaaagga gaatcagggg
     5101 aaaaagcgga gcgtccagct tttcgacagc ttagagcttg ggtttgtgcg tcagaagaag
     5161 agggggtgat ctatgtttgg cgttatgatc gattggcacg tcaagccaaa atagccttag
     5221 aatttattga gttgtgccaa caacatcagg ttcaagtgta ttcgatttct gaacctcttc
     5281 ctgcttcttt ctcacacgcg tcaacgaata aactatttgt gcaattattg ttcacttttg
     5341 cagaacaaca acgaaacgtc attattgaga atgttcgtgc cggactttcc tataaaaagc
     5401 aacaacggca atatttgtct tctgcggtac catatgggta tcgtttagtg aatgggaaaa
     5461 tccaacaaga agtacacgaa gcaaggattg ttcgctatat ttttcaactt tatttgacca
     5521 aaaaatatgg ctacaaaaag ctttgtcaac gattgacgca acaaaagctt ttcttccgcg
     5581 aacgtccttt tcagccctat catatttata gtatcctaaa aaatccattg tactatggcg
     5641 aaatcaaagg aggatctttg gggaagtatc taggaacctt tgaaccgatt ctctcgaaag
     5701 caaccttttt acaagcccaa gagattcgcc aatcaaggtg tacagctaaa aaagatacgt
     5761 atctgtactt attacgtcaa aaaattcggt gtcccttctg tggtcgtcat ctgagttcga
     5821 agtatcaatg gaatacaaag aagacgaaga cgcttcatta ttatcattgt accgaccgaa
     5881 gctgccaagg aatttttctt cgggcagaaa gggttgagtc agaagtattg gaaaagatac
     5941 aggctttttt tcaatacgaa ggcgtctatc aagccttgat tgcccaatta acgcaacaat
     6001 tagccgaaaa gcgaaaagat gaaaaacgca aagtaatcca acaaaagaaa caagaagcac
     6061 aatggattaa ggcatttgaa gagggcaatt gttcactgga agaactcaag caacaactgc
     6121 aagctcttca aaagccgatg tctacttctt cgtttctttc actccaacag tgtgaagcaa
     6181 aagtgaaaga atggcttcaa ctaaaagacc tctctagtcg acaattatta tgtgactatg
     6241 tttgtcggat tgaggtaaca aaggataaaa cagtttgtgg gatttacttc cgacaactga
     6301 cacaaaatat tttagaaagg aacgaatgat ctatggaaat gatacaaacc aaacgagtcg
     6361 cattgtatat tcgcgtgagt acacaagaac aagctttaga aggacatagt attcaagcac
     6421 aagaacaaaa tggacgacag tttgccgaac ggatgggcta tgaagtggta gaagtttatg
     6481 ccgatgaagg aatcagtggg aaatccacaa aaaatcggtt cgcctatcaa cgaatgatga
     6541 acgatgctcg gaaccatcgt tttgatctac tagtgatttg gaaattgacc cgtctcggac
     6601 gcaatatgtt agatgtctta caaacagtcg aggaattatt gcgatatgac gtaggattac
     6661 attcgatctc ggaacaattt gacgtgacca cttcgacagg taaattgatg ttacaactcc
     6721 ttgggagttt cggtgaattt gaacgcaacc aaattgcgga gaatgttcaa atgtcaatgc
     6781 aaagcttagt gaaagataaa aagcgttatg caggaggccg acgtttaggc tatgtcagtg
     6841 gtattgacac gaatggtcaa aaacaattgg tcattgaacc agaagaggcg aaaattgttc
     6901 gtctgatcta tgcaaaatat ttaggcggag cagggtatcg agcgattgcc aattttttga
     6961 atcgtcaagg atatcaaaca gtcaaaaaga atgctttttc taccacggca gtcaaagata
     7021 tcttacgcaa taaaatttat gcagggtatt tagaatatgc gcgctacatc aattgggaaa
     7081 ccaaacgacg gaaggggaag aatccgagac cgattttagt gaaaggaacc catgaaccga
     7141 ttatttcgga agaagattat caaaaagtac aagaacgcct tgctttagaa ggaaaacaac
     7201 ccaaatggaa ccatacaggt gaaaatgtat tgacaggctt gttacgttgt ccagaatgtg
     7261 gcgcaccgat ggcagcgagt aatgtcacaa ataccctaaa ggatggcaca aagaaacgga
     7321 ttcgatatta ttcttgttca gtctttcgga ataaaggagc aagtgtgtgt catgccaatt
     7381 cgattcgtgc cgaacaagcg gaaacatttg tcgccgaccg attaaaggag atcgttcagc
     7441 taccagaagt tcttccccga ttggtggcag cgttaaatga agaaatagtg cgacaaagcc
     7501 aaccattgga acaagaactc gtggtgttac tggaacgaaa ggaagaattg aagactaaaa
     7561 ttgaaaaatg ggaagctgct ttggaagata gtcctgaatt gtttccgatg ttaaaagacc
     7621 gcttagacga attgactgaa aaacgacgac agttgcacat tcgagaaaat gaaatcttag
     7681 ggatctttca acaaggagag ccgattcaag tcaaagatgt ccaacggata ttgactaatt
     7741 tggatcgttt tcttgcccag agtgagaaga agcaaatcaa agccttgtat cgtaccttta
     7801 tagagaaaat aacctttgat cctaaccata aggagatgtt ggaaattacg atgaaattca
     7861 cgcatgccgt ggtacaacaa ttgaatgagc aatatcaaat agccgtatcc aaaacaaagg
     7921 atacggctat ttttgtgtta aaaacaccct ttacgttaac gatctagtct aggttatatc
     7981 aaacaacgtt agaggggcgc taacgcttct ctagcgattt gaaatcgtta gagaagtagt
     8041 tagggaggtc cttatcataa atagggatat ttattaatga atatttgaaa tataattgta
     8101 tataagaatt aaaaagagtg gggatagata tgaaaaagat gggtaagtgt attttatgtg
     8161 aaaaatacac tgaacttact aaagaacatg taccacctaa aaaatcaggg aatactggag
     8221 gtaaaaagac cagaactggt aatttagatg atttccttaa gagcgatttt acaaaaggtg
     8281 attttccgaa ggggattaaa aggaaacctc aaggaaatgt ttattata
//
LOCUS       DAJSBL010000088         7633 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.090, whole genome shotgun sequence.
ACCESSION   DAJSBL010000088 DAJSBL010000000
VERSION     DAJSBL010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7633
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.090"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(63..791)
                     /locus_tag="ONT85_002600"
     CDS             complement(63..791)
                     /locus_tag="ONT85_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR2379535.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(804..2084)
                     /locus_tag="ONT85_002601"
     CDS             complement(804..2084)
                     /locus_tag="ONT85_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HCR2379536.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIDDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            complement(2225..2827)
                     /locus_tag="ONT85_002602"
     CDS             complement(2225..2827)
                     /locus_tag="ONT85_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HCR2379537.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFALEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            3072..3278
                     /locus_tag="ONT85_002603"
     CDS             3072..3278
                     /locus_tag="ONT85_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2379538.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            3590..4309
                     /locus_tag="ONT85_002604"
     CDS             3590..4309
                     /locus_tag="ONT85_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HCR2379539.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTNK"
     gene            complement(4390..5073)
                     /locus_tag="ONT85_002605"
     CDS             complement(4390..5073)
                     /locus_tag="ONT85_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HCR2379540.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            5291..7477
                     /locus_tag="ONT85_002606"
     CDS             5291..7477
                     /locus_tag="ONT85_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HCR2379541.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
BASE COUNT         2736 a         1284 c         1438 g         2175 t
ORIGIN      
        1 aaaaagaacc gaagggtttg gatcggctgg tcatttagtc ggtccaaact attcggttct
       61 ttttatgaaa tcaatttctt taatccacgg atagaggctg ggatggtctc gccgtttgcc
      121 aacgtgactt ctctggtttt tttattaatc gaccgcacat tttgtgtatt gataacatag
      181 gatttatgac aacggataaa attccgatgc attttctcga tttctttgat cttctcatag
      241 aattgtaatt gggcatgagt caaatggact tccagtttat gtggcgctcc tagcgaagta
      301 gaaaaatatt ggatgtcttt gatcggtaaa aatttgatct catcattaaa ctctattttg
      361 aagatatctt tttggatttg ggacatttga aaatcttcga aaattttgct caggatatgc
      421 tcgacatttt cttttaaatc ataaagatct tctttgacga tataatcagt cggttcgatg
      481 ttgcttttta agattttcat cgacatttcc gtatgggaag tcacatagat tatttttcct
      541 aaaggatcta attcacggat caatttaccg atcgctacac catccatttc actgtcttct
      601 agttcgatat ctaaaaagta aataccaaaa gaagaagttc tgatcttggc tgcattgaga
      661 agttcttccg gatcagaggt agaaatatgg acataactgt ctaagttttc aatcatgatt
      721 ctatttttga tcaccgttga taaaacttct aattgttttg gatcgtcttc acaaatataa
      781 atgggaatca tatcattact cctttatttt atttgtatat aaatcgtttg agaaaaacgc
      841 tggttttcaa ttgatgtttc aaggaaaaga taaggattgg tctttgtcaa ttcatccaag
      901 ataaacaagc caaggccttc gttttttgga tttgtttttg ttgagaaccc tttttgtttc
      961 aactggttta acggtaatgt atttgttgtt gtattgatca cacgaatcat cacggaatga
     1021 ttcttcttga gtatggtcaa ttcgattttt ttcttctttg cagcactagc tccttcaatc
     1081 gcattgtcca agataatacc aagcatacgt acaaggttta ccgtatgttt cgtatccagt
     1141 tcgattggct caggaaaatg aagctggacg atcaaccctt tttcctgtgc catcatgagt
     1201 tttaatgaca gtaaactacg gatctcagga actttcatat tatccaacat acttagattg
     1261 gccggaaaaa ggttcatcat ctggcgtgta ggtaatatcg tttgatcata ataagaccgt
     1321 aatccttcta agtcgccttc tgaaatatag ctgtgaagag aaaaaagcag attttggtaa
     1381 tcatgtttga attctctcaa gtcttgatag tttttttcta gatctttgat ataatcctgt
     1441 tgcatcctga cttgttgctc taataactga tcagctaatt tcttattaga gattttccaa
     1501 taataaacca agatgatcag gccaacacac agatatagaa tgaaaaaact cgtattcaaa
     1561 gctaagacat caggtgtttc accagaaaaa cgggtataaa aaataatgat atagtatgta
     1621 aataatatca atgtcccgat cgtaccgatc atacggcggt ccacagtgtg aaaccaagtc
     1681 tctaacgatt tttttaaaca aaaagctaat aaagcagata aaaaagcgga taagacaatg
     1741 tgaaaatact gcaatgaatc tcctggttca ataaccgaag atttcaaaac aaaaaaatcg
     1801 tcgatgcttg ttaagtgatc tcctaaaagc gacgtcaata acccaaaagg aagcgcgtaa
     1861 accaattgtt gacgcgaagt cgaaaagaag aaaaaagtaa gcatcagcat tcctatagca
     1921 attagagcag taaaaatccc taaaatcgaa tagacccatc caatcaaaat acaagaaatc
     1981 agaatgactc ccgcaaacag atatttgtat cggatgtgat ggtcaatcaa taagatacaa
     2041 attgtgtaaa tcaaatattg caacgctatg gaccataaaa acataaagta cagctcccat
     2101 tgatttttac caaattatag cacaattagc gtattatttt tttaattttt cagggatttt
     2161 gaattcagaa ctcataccaa agcagcagcc agatacaaaa aagtcagata agttagttaa
     2221 ttgtttaatg attgtttttt tcatgaaaga ttcctccaaa attcatcaag tagtttgtaa
     2281 taggaagcaa cgtagctatt tctaaaacaa tcccaagtag caaataggta gaaaagtaag
     2341 aagctgagaa aaataaagta atgagagaaa aaattagaaa catgctgatc gccttcttta
     2401 ataatggggg acgtttctcg ggttcaatgg gattgattgc tgtacctctt gatccatagc
     2461 gaagcagtaa caaaaagcag ctgattaaca aaaagaccat cattgggaag gagatagtca
     2521 attgcagatg tttcatcacc aagggaacgc cccataaata aagtagtgtc cacagcagac
     2581 aaacgaattc agagtcggaa tgcaatccat aagcatatct gcggatgcag atataagaaa
     2641 gaaaggtgat tcctgtttcc gcccatgagc ctgtcaccag agaaaagact agaacgaccg
     2701 ctagttttcc aagattattc aataaaactt ctaaggcaaa ttttacttgg acatacacca
     2761 tatcttcttc cgatttgtct ccgctgaata aggcttctaa taatttgttc acaactttat
     2821 ctttcatctc atacgtacct ccaccacaaa ttttatgcta aaaaaaataa aaaacgtctt
     2881 ttgtttcata aaaagtggtt ttgacatcaa aaccgtcatg atctcctaaa aaaatcttcg
     2941 ttttccaaga accttaccaa aaaaagtgac agttttgata cgaaaagtac tttttatgac
     3001 aagtttgtgc tttttcgagt gatttatgtt aaaaaattac tgaccttata aaaggtgatg
     3061 gagggattct tatggaaaat gaacaattgt ttgaagtcgg cgaaacatat gcatttaaat
     3121 ctgatttatt tgaatcacca ataaaaggaa aaatagaaaa aatctatgaa aatagtgtga
     3181 tgatacgtgt catgaaatgc gcagtagaag acaatgatac cgctcataat ctgcactggc
     3241 agttagttgt acgcaaatca agtacaatag aagaataaaa acagataaaa tgttgagagc
     3301 ataaagaggt tgtgagtctc ccgtttagct ctgagttaca aggaataatg ttaaaaaaca
     3361 gcttttcatg ttttatgaat attgccttgt gaccgaggtg ttggcaggag aatactgttt
     3421 atttgaaaaa agggctgcga caagactttt gtcacagcct ttattttcat gaataggagt
     3481 tacttttggc tacttttaga gaagtagctt ttaaggagca gataaagtca aggcttttat
     3541 ttctataaat gaagtagtaa aatgaagaac agattttatt acaggaagga tgccgataat
     3601 tgtgatcatt gttgcaggaa cgatcggtgc agggaaaagc acactaactg aaatgcttgc
     3661 ccaagattta gaaacaaaac ctttttatga gaacgtggaa gataatgaag tattgccgct
     3721 attttattct aatccagaaa agtatacttt tttattacag attttctttt tgaacaaacg
     3781 ctttttggca atcaaagatg ctttcagcca tgatgacaac gtactagacc gctccatcta
     3841 cgaagatagt atgctgtttc atttgaatgc agatttaggg cgtgtttctg aagtagaagt
     3901 aaagcagtat gaaggtcttt tagaaacgat gctgaaggag ttagaagaaa tttcgccaca
     3961 aaagaaacca gatctactgg tttatattcg tgtatccttt gaaactatgt tagcgagaat
     4021 caaaaaaaga ggccgggaat atgaacagct agaacaagat cctgaactct attcctacta
     4081 taaagaactc aatcgacgat acgaagagtg gtatgaacag tttgatatat gtcccaaaat
     4141 agtgatcgat ggggacaaat atgatttcgt agccgatccg gcatgtggag aacaaatcgt
     4201 acaagaaatc aagatgcggg caaagaaaat gacagaagag gcagatgccc agtcgatccg
     4261 ctacactaca aataacccga aagcaaaggg aatctttaca aataaataat ataaaaacta
     4321 agcaaattgc tcactcgcag atatttctct gtgagcagca tcttgcttag tttttttgat
     4381 tcattgagat caaatagtga accgattcgt atgataaaat tgccataaat gaaatgtctg
     4441 agtgagggcc atgatcaaaa ataaaatgga aacgatcaaa atcgaatggg cgccaaacag
     4501 atgatgacag aaaacaagga caatagctcc tgcgataaag gcaagaacga cacttagata
     4561 gtggaaaaat cgatcaaaat ctgctttttc tttagaaata caataatcat ataagttcgt
     4621 tgctacccct tttaaatttc cagttgtaaa taaattagtg taggcacgcc cgttgatttt
     4681 atcaaaagct acccactgga tcgatgcagt aaaagcaatc aaaatcgtta ctaatgcggg
     4741 atgctcatgg acgggtgtca aactaacaaa gaaaaaaatc actgcttcat aatatagaac
     4801 aaacaagcgc cagaaatgtt tttctttttt tctaaaataa tcaattagat actttgttag
     4861 aagaatccct aagaaaaaga aaagggtaga caatactttt ttaccaacac ttagccattc
     4921 tccgtcaaat gcttggatga tcgatagaat gatattgcca gtttgagcag aggcaaaagc
     4981 accatattgg atataagtat aactgtccat ggctcctccg ataaatgtca gcaaaagtcc
     5041 tattgtacgc ccctcgtgaa aggaagtctg catttttttc atcacctttt atgaatattt
     5101 ttgtcttcct tatcttagca taaacgattg aaaaaaagga aaaattttca gaaaactaaa
     5161 atcatatcgc ttcccatttt catttttaga aaggtctgtt agtataaaga aaaaatagac
     5221 aacgaaactt taaataacca acttgaaata aacaaattca aagaagaaag atgggaagga
     5281 aatcaatcag ttgaatgaaa aagaaattct ccgattacaa aaagaattga aacgctggga
     5341 gaacaagtat aaaaagcttc aacaagtctt tcggctacaa gaagaaatca ttgctaccta
     5401 tgaagcggct tatggcgaag cacccggact tattgaggaa gaagtacaag taaaagaaag
     5461 tgaaggaaac agctctttct ttaaaatgat ggagaaacgt tcctataatc gcttcaatca
     5521 agaacaaaaa gcagctatca catatgatat gaagaaacat cttcgaatca tagcaggagc
     5581 tgggagcggc aaaacgcaaa caatctgtgc aaaagctgtt tatctgatga cgcaaaaaca
     5641 agtagacgaa gaacgcatac taatgatcac ttttacaagg aacgctgcaa atgaactaaa
     5701 aaaacgagta gataatttca gccagagaaa aaccgctgtc catatcggaa cattccatag
     5761 tatctttttt aggatataca atgaaatttg tcgaaaattt cctgaagtag caatgcaagg
     5821 aatacaagga gacttttcaa aggatactgc acaaaaagtg aatgctgttt tgcaacagct
     5881 gatacgtaaa tataatcttt atatatttga tcaatatgga gaaaaaacga ttgcttctcg
     5941 gttagactat tggcagaata tgaacctttc agtagaagaa atggttcagt tagttaaaga
     6001 aaaatatgat tccattgata agaatgcacg acagccgata tcagaacgac tgtatggatt
     6061 gctcacggaa ctccaagaac aaaaaagaag ccagcaattg ctagagttca atgatgtact
     6121 ccaaaatctg aaaagcgctt tggagaatga ggagatccag cattatatcg gccaaaaata
     6181 tgattatcta ttcattgatg aatttcaaga tactaatcca cttcaatggg aaatcgtgaa
     6241 acggatgaca aaggaaaaca ggatcaagtt gattgttgtt ggagatgatg atcagagtat
     6301 ctatggattc agaggatcag agccagcata catcaagaac tttgaaaaag aatatcctac
     6361 caaaactctt ttcttattga ctaattatcg ttctcgggca gctattgtcc aaggagcaaa
     6421 tagattgatc agctataata aaaatgatcg gataccaaag gcgatgattc cagcacaaaa
     6481 agaagcaggt gttatggaag cctatttgtt ttctgatact aaagcagaaa gtcagtggct
     6541 aatcaaacaa attcagacat ttattcaaaa aaatgggaaa tataaggaat caatcatatt
     6601 ataccgatca gcttcacaaa cgcagcagct tgtccaatct ctgctgaaaa cgaatattcc
     6661 ttttgtactg gaagcagata gtccatatga aggtatcttt ggtatcaagt ctttccaaca
     6721 tttctatcaa aaaattgttc attggcaaac agcactgaat ctgcaaaaga aaaatcaggc
     6781 ctaccagcaa ttgctccggc agctgatggc agactgctac ctgaaaaaaa gtgaatgcga
     6841 tcaatacttt tctcctaaat atcaaaatat agccgttgca gattatattt tgtcgataag
     6901 ccccaattta aaatcgaaat ctgcagaaat ccgagagttt gaggaagcgt tgcgtaaaac
     6961 agaccagcat caagaaatct attcgcttgt tcaaagctat cttcatctgc cacgtatagc
     7021 taaggaaatc gaccagagtg atcaagaatg gatacgagaa gaagtaaagc agcatgctac
     7081 tttcagaaga ctccaagcct tatcgcaaga aacccaagcg acagctaaag aattaaaaac
     7141 ccgcttgatt gcttacagac aaggaaaact tgatgcacta tgtatccaga gcattcacaa
     7201 aagtaaaggt cttagttacc gaaatgtctt tttgattggt tgtaacgaag gaagtttgcc
     7261 ttataacgga gcgacagaaa agaaagtaat cgactttaat gaaataaaag cagaaccagt
     7321 gacgacgata gaagaagaac gaagactatt ttatgttgcg atgactagag caagaaatcg
     7381 tctgtatcta tgtgtacctc aaatgaaaaa ttcaagaaaa ttgaaagtat cgagatttat
     7441 caaagaaacc gggagtcgtg tgaaaaaagg aaagtgatag aagagagggt gtgacagaag
     7501 tggtcagttt caagaaagaa gaaggaatgt acgaaaattg cttctcaaat ttttgtgaaa
     7561 tttcagctta ttttcgaaga agctatttct ggaacacagt ttattcggaa aagggaggtg
     7621 tgacaagaat ttt
//
LOCUS       DAJSBL010000089         7625 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.091, whole genome shotgun sequence.
ACCESSION   DAJSBL010000089 DAJSBL010000000
VERSION     DAJSBL010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7625
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.091"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..582)
                     /locus_tag="ONT85_002607"
     CDS             complement(<1..582)
                     /locus_tag="ONT85_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HCR2379542.1"
                     /translation="MIRILQCVNDMHRAGLETMLMNYYRNIDRTKIQFDFLTHRPYKS
                     DYDEEIISLGGKVYYAPRLYPQNYPKYFKWMEQFFKDHPEYEIVHSHIDAMSYLPLKA
                     AKKAGIPIRIAHSHNTSIDRDFKYLLKQYYRCKLTSVLTHEFSCGKVAGDFLFRNDKF
                     TLIPNAVDAKKFYYDESIRNDVRMKLGISSQTFV"
     gene            complement(582..1718)
                     /locus_tag="ONT85_002608"
     CDS             complement(582..1718)
                     /locus_tag="ONT85_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HCR2379543.1"
                     /translation="MARKKLKICMLGHKRIPSREGGIEIVVEELATRMVALGHEVTCY
                     NRSGHHVSGKEFDQHKNKEYKGVKLKTIFTLNIKGIAAMSSSVFGGIRAAFGKYDVVH
                     FHAEGPCAMLWLPKLFGKRCVATIHGLDHQREKWNKLASTYIMLGEKCAVKFADEIIV
                     LSESVQNYFEDIYGRKTRFIPNGVKKIEIKSAGLITEKYGLTKDSYILFLGRLVPEKG
                     IRYLIEAFKDVQTDKKLIIAGGSSDTDEFANELKELAKGDERIIFTGFVQGQELEELY
                     SNAYIYTLPSDLEGMPLSLLEAMSYGNCCIVSNISECTEVVEDKAMIFKKSDVSDLKI
                     RLEEACNQSEMVKVLKNQATEFICSKYNWDKIVQETLNLYRGNI"
     gene            complement(1750..3201)
                     /locus_tag="ONT85_002609"
     CDS             complement(1750..3201)
                     /locus_tag="ONT85_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HCR2379544.1"
                     /translation="MYRKNSSGWSKHIDFILLDLFCVQLAFYISYVMRQGDWNPYVIP
                     IYRHMAIFIELENLTIIFLFEAYKNVLKRGYYKEAVASIKQSFMLLLVCSLYLITMQD
                     GNDYSRVSLFIMGVVYGLLTYIIRILWKGLLHHRMKNGEDVSLVIVTSQKIAKNVVRN
                     LKEKNYGMYKLSGVIVVDRDMYGTTIGGVPVVANADNAAEYLLQNWVDEVFINVDESV
                     PYPKELIARCSEMGLAVHMNLTKVSDSTRGKQFIGRVGDYTVITSSINVMTMRQAFIK
                     RTIDILAGIIGCIATGVIFIFVAPVIYIKSPGPIFFAQERIGQNGKTFKMYKFRSMYM
                     DAEERKAELMKENKMSNNLMFKMDFDPRIIGNKILPDGTKKTGIGQFIRSTSLDEFPQ
                     FYNVLMGKMSLVGFRPCLKSEYEKYDFHHRARIAMKPGITGMWQVSGRSDITDFEEVV
                     KLDAEYIRNWSMGLDFRILFRTVKTVLHRNGSM"
     gene            complement(3245..4009)
                     /locus_tag="ONT85_002610"
     CDS             complement(3245..4009)
                     /locus_tag="ONT85_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HCR2379545.1"
                     /translation="MIDLHCHILPGIDDGAENLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAERLFFELLELGKIPVIVHPERNAHFREDPNHLLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKTMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKEFG
                     KEKVVQMKQVAKDLINGDEINYPIYEEVKKKKFGLF"
     gene            complement(4037..4735)
                     /locus_tag="ONT85_002611"
     CDS             complement(4037..4735)
                     /locus_tag="ONT85_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HCR2379546.1"
                     /translation="MARTQKQKAAQTHAVSLVTLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVIYKTFKLNNAS
                     GLSTVLSSSGSVADEIQRTPVDNLSILPSGPKPPNPSELLSSPRMDQILAEARQLFDV
                     VIFDMPPVVAVTDAQIMSSKTDGTLLVVRENTSRKESLTKAKELLDMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            complement(4747..5526)
                     /locus_tag="ONT85_002612"
     CDS             complement(4747..5526)
                     /locus_tag="ONT85_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein kinase"
                     /protein_id="HCR2379547.1"
                     /translation="MEETISLKEIFETLRKHLVIIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     EQIKDAISVNQAENSQMFSIQAISTNAVAAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLMTGLMLGMMVGVGLAFLLELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGPAQTQPKATNDKTAPSRRSRSRV"
     gene            complement(5542..6486)
                     /locus_tag="ONT85_002613"
     CDS             complement(5542..6486)
                     /locus_tag="ONT85_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HCR2379548.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFPIGKITLNGDQALSYSRMRYEDPNGDYGRQGR
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMNFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            complement(6532..7311)
                     /locus_tag="ONT85_002614"
     CDS             complement(6532..7311)
                     /locus_tag="ONT85_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2379549.1"
                     /translation="MEKPFVKSLTAVAVGAGAIAICLFGYHINNQRQHQQRINYAESA
                     ITNQKDTLTSLSKEVDKLYSTKEKIFLNPEITEETITSLSNKLSSVKLSADDYDIKES
                     ELPKEAVAIQDEKKAIVTQLDSAESKLKIQTAVNDLFTKNVSNWQQAVDDVIIKEKVT
                     SADVARVRENMSFFKDSPWKTVVMQYLGFADIQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTVLSQIEQIRNEKISAAYATKAQTVAEQMGYSNISY"
BASE COUNT         2149 a         1609 c         1169 g         2698 t
ORIGIN      
        1 tacaaacgtc tgacttgaaa ttcctaattt cattcgtaca tcatttctaa tactttcatc
       61 ataataaaat tttttagcat ccactgcatt tggaatcaaa gtgaatttat catttcgaaa
      121 caaaaaatca cctgctactt ttccacatga aaattcatgt gtcaaaacag acgttaactt
      181 acatcgataa tattgcttta aaagatattt aaaatctcta tctatcgatg tattatggct
      241 atgtgctatc cgtatcggaa tcccagcttt ttttgcagct tttaaaggta aataactcat
      301 cgcatcaata tgagaatgaa caatctcata ttctggatga tccttaaaaa attgttccat
      361 ccatttaaaa tatttaggat aattttgtgg ataaagtctc ggtgcataat agacctttcc
      421 tccaagactt attatttcct catcataatc acttttatat ggacgatgtg tcaaaaaatc
      481 aaactgaatt ttagttcgat ctatattcct ataatagttc attagcatcg tctccaaccc
      541 tgcacgatgc atatcattca cacactgtaa tattcgaatc attatatatt tcccctatac
      601 aaatttaatg tttcttgtac gattttatcc caattatatt tactacaaat aaactcagta
      661 gcttgattct tcaaaacttt aaccatttcc gattgattgc atgcctcttc taatcttatc
      721 tttaaatcac ttacatccga tttcttaaaa atcatagcct tgtcttctac tacttccgta
      781 cactctgaga tatttgaaac tatacaacag ttcccataac tcattgcttc taacaaactt
      841 aatggcattc cttctaaatc acttggaagt gtatagatat acgcattact atataattct
      901 tctaattcct gtccttgtac aaatccagta aatataattc gctcatcacc ttttgctaat
      961 tcctttagtt catttgcaaa ttcatctgta tcagagcttc ctccggctat tataagtttt
     1021 ttatctgtct gaacatcttt aaatgcctca attagatacc gaattccttt ttcgggaacc
     1081 aatcgcccca aaaacaaaat ataagaatct tttgttaatc catacttttc tgtaatcaat
     1141 cctgctgatt ttatttcaat ttttttaacg ccatttggta taaaccttgt ttttcttcca
     1201 tatatatctt caaaataatt ttggacgctt tcactaagta caattatttc atctgcaaat
     1261 tttacagcac acttttcccc taacatgatg tatgtactag caagcttgtt ccatttttct
     1321 ctttgatgat ctagaccatg aattgttgca acacatcttt tcccaaaaag ttttggcaac
     1381 cacaacatag cacatggtcc ttctgcatga aaatgaacaa catcatattt tccgaatgct
     1441 gctctaattc caccaaatac tgaagaactc attgctgcaa ttccttttat atttaatgta
     1501 aaaatagtct ttaattttac acctttatat tctttatttt tatgctggtc aaattccttt
     1561 ccgctaacat gatgaccact tcgattatag caagtcactt catgcccaag tgcaaccatt
     1621 cgcgttgcca attcttctac cactatctca attccacctt ctcttgatgg aattctttta
     1681 tgacctagca tacagatttt taattttttt cttgccatca cgttttactc cttctatagt
     1741 atctttatct tacatggaac cgtttctatg aagcacagtt ttgactgtcc taaacaaaat
     1801 tctaaaatct aatcccatac tccaattcct gatatattct gcatcaagtt ttacaacctc
     1861 ttcgaagtct gtgatatcac ttctaccact cacctgccac attccagtaa tcccaggttt
     1921 catcgcaatt ctcgcacgat gatgaaaatc atacttctca tattctgact tcaagcaagg
     1981 tctaaacccc accaggctca tttttcccat taggacatta taaaattgtg gaaactcatc
     2041 caatgatgta gatcgaataa attgtccaat tcctgtcttc tttgttccat caggaagtat
     2101 tttattgcca atgattcttg ggtcaaaatc catcttaaac atgagattat tactcatctt
     2161 attttctttc atcaactctg ctttgcgttc ttctgcatcc atatacatac tacggaactt
     2221 atacatctta aatgtcttac cattttgacc aatacgttct tgtgcaaaga aaatcggccc
     2281 tggtgatttg atgtaaataa ctggtgctac gaagataaaa ataacacctg ttgcaataca
     2341 tccaataatt ccagcaagaa tgtcaatcgt tctctttata aatgcttgcc tcatcgtcat
     2401 aacattaata ctagatgtaa taacagtata atcaccaact cggccaataa attgttttcc
     2461 acgtgtacta tcagacactt ttgtaagatt catatgtaca gcaagtccca tttccgaaca
     2521 tctggcgatt aattctttgg gatatggaac actttcgtca acattgatga atacttcatc
     2581 tacccaattc tgtaaaagat attccgctgc attatcagca tttgcaacta ctgggacacc
     2641 tccgatggta gttccataca tatctctgtc tactacaatc acaccactaa gtttatacat
     2701 gccataattc ttctctttaa gattacgaac tacatttttt gcaatctttt gagatgttac
     2761 aataactaac gatacatctt caccgttttt catgcggtga tgcaacaacc ctttccaaag
     2821 tatacgaatt atatatgtta ataatccata caccaccccc attataaata aagaaactct
     2881 ggaataatca tttccatcct gcattgtaat aaggtataaa ctgcacacaa gtaacagcat
     2941 aaaagattgc tttatagaag ctacagcctc tttataatat cctcttttta aaacattttt
     3001 atatgcctca aataaaaata taatcgtaag attctcaagt tctataaaaa ttgccatgtg
     3061 gcgataaata ggaatcacat acggattcca gtcaccctgt cgcattacat atgatatata
     3121 aaaagcaagc tggacacaaa ataagtctaa taaaataaaa tcaatatgtt tagaccaacc
     3181 acttgagttt tttctataca tctctcactc tctcctcatt tcctttttat ctacgaataa
     3241 agaactaaaa taaaccaaac ttcttctttt ttacctcttc atagattgga taattgatct
     3301 catccccgtt gatcagatcc tttgcgactt gtttcatctg tactactttt tccttaccaa
     3361 actccttggc aatctgctca tagcactctt ttaagtaaaa ggtgcgctta gagaccccgt
     3421 gtgcatcaga agcagccatc tgcacgagat tatgtttgac cattgttttg gctgttttct
     3481 ggatatcttt gccaaataca ccaacataac ttggcgcagt aagttgggcc aaacatccca
     3541 tatctaggaa cggaagcaaa tgatttgggt cttctcgaaa atgcgcgttg cgttctggat
     3601 ggacgatcac tggtatcttt cctagttcta acaactcaaa aaaaagccgt tctgcatata
     3661 acggcacttc cagtgtcgga aattcgatca gaagatatgt atcatctaaa tctgtaaata
     3721 aaatttcatc tcggtggatg tcttcgatga gcgttccggt aatacgtact tcttgtcctt
     3781 ccaataacgt caaaggcaac tgtcttttct ctaattcggc ttgtaacgaa gcgactagag
     3841 aaatgacttg tgatttttca ttgctgtatt ttccattatt gtggtgtggt gtgcataaaa
     3901 tatgcgtaat gccttgctga atcgcttttt cagccattgc aatacttgct tctaagtttt
     3961 ctgccccgtc atcgattcct ggtaggatat ggcaatgtag atcgatcatt tctcttgtct
     4021 cctctcttct tattccttaa tttccgtagt aataaccagc atctttgctg tgttctgctc
     4081 cgttgtatac gacacctaag actcgtgctt gtaccatatc taacagttct tttgctttag
     4141 tcaatgattc ttttcgtgac gtattttccc gtacgaccaa taaggtgcca tccgtttttg
     4201 aagacatgat ctgtgcatct gttacggcaa cgactggcgg catgtcgaag atcactacat
     4261 caaatagttg tcttgcttct gctaaaatct gatccattct tggcgaactt agtagttctg
     4321 atggatttgg tggtttaggt ccacttggca gaatcgacaa attgtcgact ggtgttcgtt
     4381 ggatctcatc tgctacactt cctgatgaac taagaacagt acttaatccg cttgcgttgt
     4441 tcaacttgaa cgttttatag atgacgggct tacgtaagtc tgcatcgact agcagcaccc
     4501 gttgaccaga tttggcaaaa acaacggcaa tgtttgctgc agtggtcgat ttcccttctc
     4561 caggtcctga tgaagtcaca acaatcgttt tgatttgctg accagctgcg gaggcaaact
     4621 ggatattcgt ccgaatcgta cgatattgtt cagcgattgg tgaagaggga ttggtgagtg
     4681 tgaccaaact gactgcatga gtttgggctg ctttttgttt ctgtgttcgt gccattttct
     4741 ttcctcctag actcttgagc ggcttctgcg tgatggcgct gttttatcgt ttgtagcttt
     4801 tggttgtgtc tgagctggtc cttttttacg gataccagca ttcaattctt tcgctgacat
     4861 ttctggaact gttcctaaga ttgggaatcc taatgtatct gtcacatatt tttcatcttt
     4921 cactgtccga tcgagtaatt ccaataagaa tgccagacct acacctacca tcattcctaa
     4981 cattaagcca gtcattaaat tcaatttatt atttggcgaa acaggtgtcg tattggcaac
     5041 tgcacctgaa atgatcgaga ttttatcaac attcatcaca tcttttgcgt tctcttggaa
     5101 aacttgtgaa gttgtgttag cgatctgttg agcagccact gcattcgtac ttattgcctg
     5161 aatcgagaac atttgtgaat tttctgcctg attaactgag atggcatctt tgatttgttc
     5221 agcagtcatt ttcacattgt cttctgtttc taaacgatct ttgacttcat tgagcactaa
     5281 atcactgacg atcatatctt tatacgtatt gatcatttgc aggttcgtat tgacatcatt
     5341 gacatttgca gtgtctgatt gtggcagtgt cacgatcaat tgtgcttgtg aactgtattt
     5401 tggtgtcaag atgaaaaatg ttacgattcc tgagatggcc aagccagcaa acattgagat
     5461 gatgattatt accaagtgtt tgcgtaatgt ttcaaaaatt tctttcaagc tgattgtctc
     5521 ttccattttt tctccacttt cctatttatt ctctagattc atttgatctt ttaattcttt
     5581 tttcacacga tcaagttctt gatcactgac tttttgatag gagatattat cttgcatgaa
     5641 gccttctcct tgcagctgat cttgcttaat cgtaccaaat gcctcccgat aatctaaagc
     5701 aatttttttc atttcatcaa agttcatgtc tgttttcata ttcgactcta gagcattcaa
     5761 aacggattga tagttgctga caccgtttag actcaatact tttttcgcta cgccttctac
     5821 gatctttctt tggcgccctt gtctaccgta atcaccattt gggtcttcat aacgcatacg
     5881 agaataagat aatgcctgat ccccgttcaa tgtaatttta ccgattggaa agtcatagcc
     5941 atcttgtgag aaagtgaggt cattattgac ttcgatacca ccaactgcat caacgagttc
     6001 ctttagccct ttcatattga tagagacata gtgatcgatt gggatgtcta catatttttg
     6061 tacggtatcc atcgccatgg cagctccgcc gaatgcgtaa gcgtggttga tcttgtcttt
     6121 tgtgtcacga cctacgatgt cgacatacgt atcacgagga atgctgacga tcgttgtctg
     6181 tttgtcttca ggactgactg tggcaaccat catcgtatct gatcttcctt gatcgacccg
     6241 acctaaatct ccagtatcaa tccccatcaa tagaacagag aaagattctt gtttgtccag
     6301 atcaactggt ttcgttcgtt ctccttgggt tcgttccact gattcgtaag ttttctcaat
     6361 agaatgtgag agatccagat ataattttgc tcctactcct acaattgcta atactaatac
     6421 gataagaact gctaaaataa tcagaaatat ttttttccct cttgattttc ttcttcgtct
     6481 tgccatagtt tcctccgttt gctaaccttg cttgaatcaa cgttttaact gtcaatagct
     6541 tatattgcta tagcccattt gctcagcaac agtttgtgct tttgttgcgt atgctgcgct
     6601 tattttttca ttccggatct gctctatttg actgagcacc gtcaaatatt gatcgtaagt
     6661 agcggtggca gtcacttgac catcctttaa catcgtatcg aataactgat ctagttgcgt
     6721 gacttgcgcg atctgaatat cagcgaatcc tagatattgc ataacaaccg ttttccaagg
     6781 actatcttta aaaaagctca tattttcacg tacacgagcg acatctgcac ttgtcacctt
     6841 ttctttgatg atgacatcgt ctacagcttg ctgccagttt gatacatttt tagtaaataa
     6901 atcgttgaca gctgtctgga tcttcagttt tgattctgca ctatctaact gtgtcacgat
     6961 tgctttcttc tcatcttgga ttgcaacagc ttctttaggc aattctgatt ctttgatgtc
     7021 gtaatcatct gctgaaagtt tgacagagga tagcttattt gacaaacttg tgattgtttc
     7081 ttctgttatt tcagggttta gaaaaatttt ttcttttgta ctatacagct tgtctacttc
     7141 ttttgataaa gaagtcaagg tgtctttttg attggtgatg gcagattctg cataattgat
     7201 ccgttgctga tgttgccgct gattattgat atggtatcca aacaaacaga tcgcaatagc
     7261 acctgcacct accgccacag cagtcaatga cttgacgaat ggcttttcca tagattcact
     7321 cctaagtatt gatgatagat aaattaagat ataattcact tacataaaaa tgacagcttt
     7381 ttatttaaaa aaacataatt aaactaatat attcacagaa atatttgtaa acaagttttc
     7441 cctatataat cataccacaa actgttttct tgactagggc aatttacgat ttaaaagtaa
     7501 ataaaacatt aataagataa aaattaatcg atttcatttt tatttccata attttcatag
     7561 gtgtttttaa aagttacaca aaaaatagaa taacaaaaaa attaacattg aattaataat
     7621 agtta
//
LOCUS       DAJSBL010000090         7600 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.092, whole genome shotgun sequence.
ACCESSION   DAJSBL010000090 DAJSBL010000000
VERSION     DAJSBL010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7600
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.092"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(943..1356)
                     /locus_tag="ONT85_002615"
     CDS             complement(943..1356)
                     /locus_tag="ONT85_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HCR2379550.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            complement(1812..2297)
                     /locus_tag="ONT85_002616"
     CDS             complement(1812..2297)
                     /locus_tag="ONT85_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2379551.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(2436..2651)
                     /locus_tag="ONT85_002617"
     CDS             complement(2436..2651)
                     /locus_tag="ONT85_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HCR2379552.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            2875..3675
                     /locus_tag="ONT85_002618"
     CDS             2875..3675
                     /locus_tag="ONT85_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR2379553.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIKEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            3694..5562
                     /locus_tag="ONT85_002619"
     CDS             3694..5562
                     /locus_tag="ONT85_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HCR2379554.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            5555..6046
                     /locus_tag="ONT85_002620"
     CDS             5555..6046
                     /locus_tag="ONT85_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HCR2379555.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            6034..6588
                     /gene="srlA"
                     /locus_tag="ONT85_002621"
     CDS             6034..6588
                     /gene="srlA"
                     /locus_tag="ONT85_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327877.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HCR2379556.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            6606..>7600
                     /locus_tag="ONT85_002622"
     CDS             6606..>7600
                     /locus_tag="ONT85_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HCR2379557.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVPTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2708 a         1213 c         1442 g         2237 t
ORIGIN      
        1 tttgttacta tgaataacat aataagaaag taaagtggat tgatttgaga tctttttata
       61 cttcaaaaaa gataacaccc aactttcaaa aatatgtttt ggaagttggg acaaacgagt
      121 taatacattt taatacatta ttttttattc aaaaaaaata gcacaatggc aaagatcgta
      181 agagagatca taagccccaa tgaatgacta aaaagaaaag cagaatcaca tgccatatca
      241 tcccatattt cttcaagaaa ataaataagc gggaaaaagg gaaggaatag tagaacatcg
      301 atccagcacc atttttttct tcttttgaaa taacataaaa caataaacat gaacgtgcag
      361 ggaagtaaaa acaaagcttt actcataact aacaaaatac cagctccttt tatttttttc
      421 tcccccacat tcaaagagta acaatggtat gtacttaata atagaaaaat ggatcaactt
      481 gtatgaataa cagctgaaaa gatctgcacc aaataaaaaa cttccagcca aaactgactg
      541 gaagtttatt tttttctgaa atattctaac acaaatatga aaaagatggc cgaagctaat
      601 aagccgattt cgtagcaaaa caatgagata ccagagtcgt aataataaaa tcctctaatc
      661 ttatggataa aatagatcat cggacaagca gataagagta agtatttgtt aggccttttg
      721 aataagtaaa agaaagcact ggtcattatg ataaatggta tccaaaacaa aagtttactt
      781 aagacaatca aatgatcaac tcccatattt tttaccaaga gagatcataa catctttatc
      841 gtaaaaacaa aatatttttt tgctcatttt gaaaatttaa acatactcgt tcttatccaa
      901 atatataaaa atcgcccctc attcatcgag aggcgataag ttctaattta actcaatttt
      961 ttttgtttct tttggctgat aagctgtttt aggtaatacc aatttcagca caccgttttt
     1021 aaaggttcca gtgatttttt cttcatcaac attaggaaga tagaatgaac gtttgtaaga
     1081 gaccgaatgt ctttctttcc gtacatagtt gccatcttct ttatcttctg tatgggactc
     1141 atgattggcg gagatcgtca aagtattatc tgaatattca actgtcgtgt cttctttgtc
     1201 acagccaggc atatcagcag ttagtttgta ttctttttca ttttcgacta aatctacttt
     1261 tggataactt gaaacaccta aaaaatcatt gaatgctgga ctaaacacgt cattgaaatc
     1321 agggaacatg tcgcgaatgc taggtacatt tgccattttt gatcatcctt tcaataagta
     1381 ttttttattt atccctattt acgtaataac tctattttga aaaaaatgca agtataatgg
     1441 ttagctttgt gaatatttca tctaatacga tacaaaggct atctttctaa tttgaaaaag
     1501 ggatcattaa aataataaga agaaaatatg tcaaaaggta aaaaagtaaa ttcttctatc
     1561 caaatataga aaaattgaat ctagaaaacc gcccgacaat aaatttcatt aactcagttg
     1621 accgaaaatg aagtagatac caaaaattta tcaagagagc aagtagagaa gtgagtatat
     1681 tcataagaga aatagctgta acgaattatt aggttaatgc aaatgcttat ttatactccg
     1741 tataactttc tcagaaaaaa tgataccaat aatgacttgc tagtactgta aatccgtata
     1801 atatcatcta atcaaaatgg tgttccattt gcatctgtca tcgtaatcga tccttcgggt
     1861 gacactcgat aaaaaccaac gcttccagat cctccttggg aacggatttg tttgctggaa
     1921 gctgtgaaca aataatctcg atcaattttt tgataaaagc taataacaat atcgtcactt
     1981 tgtaattcgg aacggtgggc aataagaaat agaagtgctt tgtcagagtt ttctaatgga
     2041 agagaaggta cagtgctttc ttgcatttca gatgttgaag aaatgctaaa cgactgaata
     2101 cttgattggc tatgttgcga atctacatta ccagtagaag tattagtaat ggaagtagta
     2161 gaagtacttt tagttgaacc aacctcagtt gttgaaatgg ctgctgaaga ttctttaaca
     2221 gaattctttg ttttatatcc acaggcagaa aaaagtatta cagttcccaa caagcctgcg
     2281 taaatccctt tatgcatgat agctcctcct aaaatagatt atctttattc agtatatcac
     2341 ttaaacaaag aaaagcagat ggtttaaaat gaaaaaacct tcccaataaa gggaaggttc
     2401 atgaagataa taactttttt aataattata gctttctaat atctttcatc aacttttcta
     2461 gtaatcattg ggaaaaattt caagcagtag atcgcacaaa tacctacgac gatataaatg
     2521 atcttcgcaa aaatagttgt tgaaccacca gcaatcatag ctactaaatc aagttcgaac
     2581 aagcctacca acagccagtt caaccctccc acaatcaata aagttaatgc aatagcatct
     2641 aaagttttca ttagaattct cctttcttaa atgataaaac aattatatat caaaaagaga
     2701 aacattgctt atataacgct caggaatttt agcaatctat gcaaaaagat gaaaattttt
     2761 tttgctcttc cattagtaca cattttttag ctgaagctgt aaatttcatg tcttaatttt
     2821 ttttagtgag cttttatact ggatttgtaa gaaaaaagaa ggagaggatc acatatgtcg
     2881 gattggttgg atattgcagg aaaagtagta attgtaactg gaggttcctc aggaattgga
     2941 agaagtatcg tcgaaaattt gttgaagcag aatgcacaag tagcgaattt tgatgtaact
     3001 gaatgccgta tacagcatga aaacctacta tctttaaaag tcgatgtgtc ttctaaaacg
     3061 gacataaaag aagggatata taaagtaatg aaacatttta agacgatcga tggtctagtc
     3121 aataatgcag gaataaatat tccttctctt ctaatagata gaaatcatcc aaaaagcaag
     3181 tatgaattat cagaacaagt ttttgataag atgatcgctg tcaatcaaaa aagtgtatat
     3241 ttgatgagcc aagcagttgg aagaatcttg gtgcaaaagg gatcaggagt gatcgtgaat
     3301 ctttcttctg aatcaggatt ggaaggatca gaaggacaga gctgttatgc tgctacaaaa
     3361 gcagcgatga acagttttac tcgttcttgg gcaaaagagt taggaaagag gaatgttcgt
     3421 gtggtaggtg ttgcaccagg gatactggaa gaaaccggtt tgagaacaca agaatatgaa
     3481 gaagctcttt cttatacgag aggtataagc gtcgaacagc tgagaaacgg ttacagtcga
     3541 acaagtacga tccctctagg aaggagtggt aagctgcaag aagtggcgga tctagtctgc
     3601 tattatcttt cagaaagatc aagttatatc actggagtca cgactaatat ttcaggaggt
     3661 aaaacaagag gataaaggat aggggggacg cacgtgacgc tagtaaacag atggtaccaa
     3721 ttattgaaat tactagtcac tcacaagaaa gtttcaatcc aggaagtgca aaaagaaatc
     3781 aaaaccagta atcaaaccat aaaaaaagat attggggagt tgaatgaaga aattgccggt
     3841 attgcaaaaa tcatccaaaa aaacaaacat tttgaacttg aaatttatga ttttgatttt
     3901 tttgatgaga tcatgcaagg aaaattgaaa acagccagtg atttcaattc ggcatccaaa
     3961 agaatcgctt atttactaaa taggctgatc gaaacagagg atttcttacg tatggatgat
     4021 ttgtctgaag aagtcggtgt cagcagagga acagtggtca aggatctaaa gacattaaaa
     4081 gagatgatca atgattttga tgtaaagatt acaggcacac caaataaagg actaagaata
     4141 ataggagatg agctagaatt acgactactt ttattttatt ttgtttctcc ctacttttca
     4201 gagacgaaaa cagaacagtt tctagaagaa aaacaagcaa tcaaacattt tcagaagaag
     4261 acgaatgcta gtaaacaaga aatcaccctt ttaacgaaag ttatcggtat ttctttgaat
     4321 agaatctcta gtaactatct gttgaagaag cccatcaatc gttacagtgg gtgttttgaa
     4381 tatatccaag aattcaatga actgatcaat cagttggaag aaatctatga actaactttg
     4441 agccaatatg aaaaagattt tatcagtttt cctttgaaca tcatcaacac aagtataacg
     4501 atgataagga aagctaactc tgatttgcgc ttatatttcg accccatgat gatgcgtatt
     4561 cgacgattag atctcattga tctggatgag tccttcttat atcaagagat gaaacagcac
     4621 ctctctttat tgataaatcg ggtaattttt cgttatcgat tgactgattt attttatggt
     4681 gagatagaaa aaaagtatcc tttttcttac caaatcgcta gcgaatgcat acaagcatta
     4741 gaagctatct tgcaacataa gatctctaaa gtagagatca gctacctaac gttgtatttg
     4801 gagttagcat ttcgatatca aaaagagcat ttttcctcta aaaaagtagc cattgttcat
     4861 acgacaggaa aaggaacagc attaatgatc caaagacaaa tcaaaaggat attggggagg
     4921 gatatacaaa taacacatgt ttcagaagaa caatatgaaa aagtagatct aaacaaatat
     4981 ttcgctgttt ttacaacgat tccgttaaaa aatgtaaatg aagagacacc ggtgatccat
     5041 ttgactaatc tttttgatga ctcttggtta agggcacaat ggcaaaaagc aaaaaaaaag
     5101 cgattagccg ggtttcattc tactgttttt ttctatcaaa tatggaaaga aaaaaagacg
     5161 tatcaagaat atttaaacaa attagtcgct cctttgatca gaggctatta tgtggatgaa
     5221 gaatttgaaa aaaggatctt gaaaagagaa tatcagcaaa gcacatttgt taacaaagaa
     5281 tttgcttttc ctcacacaac caaccagcta tctcaacaca ttgttttatc agtagggcta
     5341 ataactgatc aaggtttagc agaaacagga ggaaatggag ttattttttt atttgccata
     5401 cctgaagaaa tgacggctga agttgagaat gaattatttg agctatacga tatcctattt
     5461 gaactcataa aaatgggaat agatgagttt attgaattag caccgacaca agaatcattt
     5521 ttaaaactaa tcaaaagtaa aggagagaga cttcatgaat gatatgtatc tattcatagt
     5581 tttcgcagct tcagcttatg tgctgcaagc agttatcagt tggaagcaac taaagcattt
     5641 caatcaaaca tatagcgaac tgcgaagaaa aggaaaagta gcgattggta aacgacctgg
     5701 gaaaatccgt tcaggcacga ttgttatgct agctatagac gatcatgatc aagttttaga
     5761 agtcagaaaa atgcaagggg ttacatcttt ggcaaaattt cattcattac ctgtctatgc
     5821 gggtgaagat atccattaca tcgatcgtta ccatccctca gttcgagcgg aagatcgttt
     5881 gacacaagca gctttagaaa atgccagaga agtatactta cgaatagagg caggaaatga
     5941 aaaaagtgaa caaccttcat tgccttttat tgctatgctc aataattttc ggatatggaa
     6001 aagtacgatc aaagaaaaaa ggagtgacaa acgatggata tcataacaaa atttgctgag
     6061 ggatttatga ggctgttcca aacaggtgca gaaacattca taagctggat gacaggaatc
     6121 gtaccagttg tcttgatgct gatggttgca atgaatacgc tgattgcttt tttaggtgaa
     6181 gaccgagtaa cgaaagtagc caaattatca gcgaaaaatc cgcttactcg ttaccttgtg
     6241 ttaccgttct tatctgcttt tatgttggga aatcctatgt catttacaat ggctcgtttc
     6301 cttccagaat actacaaacc aagctattat gctgctcaag ctcaattttg tcatacgagc
     6361 aatggtgtat tcccccatat taatcctgga gaattgtttg tatggatggg gattgctcaa
     6421 gggatcgaaa ccttagggct aaattcgatg gagctggcta tccgatactt gcttgtggga
     6481 ctgctgatga actttattgg tggttggata actgatttta ctactggatt tgtatgcaga
     6541 cagcaaggaa tcgttcttag caaaaaagta gagctttcag ttgattagag aaaggagagt
     6601 tttaaatgta tggtgatatt aagatcgtaa gaggaaacgg cgggtatgga ggtcctttga
     6661 ttattaggcc gacaaaagat aaacacaagt tcatctatgt cacaggtgga ggtgagaggc
     6721 caatgatcgt agataaaata gaagaactga caggtatgga gtctatcaat gggttcaaaa
     6781 catcaatacc tgatagtgaa atcgcgcttg caatcgttga ctgtggaggg actcttagat
     6841 gtggaattta tcctaaaaaa gggataccaa ccatcaatat cgttcccaca ggaaaaagtg
     6901 gcccattagc acaatatatc actgaagata tttatgtttc agcagtaggg ttagaacaaa
     6961 tacagccact aatggaaact gatgataaca aagaaacagt aaatactgca tctccagata
     7021 aaaagtacaa atatgatact tctaaaaaga taaccgagca aaaagcagag cagcaaaact
     7081 ggatagcaaa aattggaatg ggcgcgggaa aagtagttgc tacattcaat caatcagcaa
     7141 gagaagcagt agatacgatg atccggacag tgatcccttt tatggctttc gtttcccttt
     7201 tgattggtgt gatacaaggt tctgggatcg gtaatgtatt tgctaacatt atggcacctt
     7261 tagcaggaaa tgcagttgga ttaacaatca ttggctttat ttgttcactg ccatttttat
     7321 ctcctttgct tggtccaggg gcagttatta gccaagtgat tggaacattg atcggtgtag
     7381 agattgggaa aggaaatatt cctccccaat tagcattacc ggcactattt gctatcaata
     7441 ctcaaaatgc atgtgatttt attccagtcg gtctaggttt ggaagaagca aaagctgaga
     7501 cagtagaagt aggtgtacca tcagttcttt attcaagatt tcttaacggc atcccacgag
     7561 ttatcattgc ttgggtagca agttttggat tatatagctg
//
LOCUS       DAJSBL010000091         7520 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.093, whole genome shotgun sequence.
ACCESSION   DAJSBL010000091 DAJSBL010000000
VERSION     DAJSBL010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7520
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.093"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(201..878)
                     /gene="nth"
                     /locus_tag="ONT85_002623"
     CDS             complement(201..878)
                     /gene="nth"
                     /locus_tag="ONT85_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HCR2379558.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(885..1583)
                     /locus_tag="ONT85_002624"
     CDS             complement(885..1583)
                     /locus_tag="ONT85_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HCR2379559.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            1851..3035
                     /locus_tag="ONT85_002625"
     CDS             1851..3035
                     /locus_tag="ONT85_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HCR2379560.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            complement(3041..3484)
                     /locus_tag="ONT85_002626"
     CDS             complement(3041..3484)
                     /locus_tag="ONT85_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCR2379561.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(3710..3997)
                     /locus_tag="ONT85_002627"
     CDS             complement(3710..3997)
                     /locus_tag="ONT85_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2379562.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
     gene            complement(4168..5520)
                     /locus_tag="ONT85_002628"
     CDS             complement(4168..5520)
                     /locus_tag="ONT85_002628"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidase"
                     /protein_id="HCR2379563.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            complement(5793..6239)
                     /locus_tag="ONT85_002629"
     CDS             complement(5793..6239)
                     /locus_tag="ONT85_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HCR2379564.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            complement(6475..7401)
                     /gene="cysK"
                     /locus_tag="ONT85_002630"
     CDS             complement(6475..7401)
                     /gene="cysK"
                     /locus_tag="ONT85_002630"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HCR2379565.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
BASE COUNT         2030 a         1543 c         1235 g         2712 t
ORIGIN      
        1 gaaggcatta ttttctacat cgggtatttt cccaataaaa ataacaactt ttatttttat
       61 cgcaataaat tgtaataagt taccatccta gctactgagt atgtacattt actttttcat
      121 gcaaaaaaga taaacgaaac agcttttctc cataataaca agataattca tacatttatt
      181 tgcaattaaa gaaaccacgt ctacaggcgt ggtttctttt ttagcgcctt ttcttttaga
      241 cgcatccgat ttttcccatc ttgacatatt gatagcaaag gacatacctc gcacttggga
      301 tttcttgccg tgcaatgata tctaccaaaa aaaatcagtg tatgatgtgt ctttacccat
      361 aattcttgtg gtactttccg catcagtgtt tcttctactt ccatgaccgt agcatctagt
      421 ttgcagatcc tcaggcgttt tgagacacgt tccacgtggg tatctactgc gattgctggt
      481 atcccgaatg catcccccaa tacgacattt gctgtctttc tgcctacacc aggaagagac
      541 atcaattctt ctcttgacgt tggcacttgc ccatcgaaac gttcaatcag ctgctgtgca
      601 cacgctttga tgttttttgc tttatttcga tacaaaccaa tcgtttttat tttaagaatg
      661 atttcatcga ttgaagcttc tgctaacgca tcgggtgtcg gaaaagaggc aaacaaatct
      721 ggcgttgctt tattcactga tacatctgtc gcctgggcac ttaagatcac tgcaatcaat
      781 aactcaaatg gattattgtg cttgagttct ccatgtgctt cgggaaacat tccatacatc
      841 gtttctagag cttccattgt tttttgtttg cttaacatat tccgctactc ctccaaccaa
      901 tttttcagtg agactttcgg caattcttct tgttgagctg actgttctac ttctctttga
      961 agaagctgct gttttcttcg tttttggtct tcttccactt gttgttttgt acgtagattt
     1021 tttctttccc acgatagcaa aatgcggtca acgtaattaa aactataagc ctgatttaat
     1081 acggcttccc gcagtgctag cttcaaaatt tccggctgat aatggtcttc ttctagccat
     1141 tgtccgatcc gctggaactc gatcgaagaa agcgggcgtc caaattcttg ttcaaataat
     1201 tgatacactg actggatttc tttttcttgt gttttttctt cgtatttttt ttcttgtgtt
     1261 tttaaatact cacctaatag atcatagatg gggtaaagat tatatcgatc tgctttttta
     1321 ccgttgacaa tcgtcgtttc tatcttgata aatccatttg ttacgagacg atccagtatt
     1381 tgaaaaatct gatcttgttt gattcccata tccatagcaa tcatctgcaa atcagggaaa
     1441 gtatctcctt caagctgtgc catatgcaac tgtagaacaa ataaaaattc ttcagattgt
     1501 agaccgatcc tatgataatt tttaagtaaa agattagata tgactgtttg tccagcgctt
     1561 aagtaatttt ctaaattcaa catgatttct cctccgctca atcacagtat acgtaaaaaa
     1621 aacatgggaa gcaagaacag gtttcttttc tatttacttc actgatcggt cagatttaca
     1681 atgtcgtcta cttgattttt gtttgttcat gtttttgatg cgtatcttca cttttcggaa
     1741 taaataaatt aagtagattt cgctgttcct tagaatgttg acaaggaaac aaaattacta
     1801 tacactattt ttgtttccaa gttaacatta ttgcataaag gagtttttac atgtacagtt
     1861 tcacaaataa acattctaaa gtttttggat acgtctctgt cttattcact ggattaggat
     1921 ttactatcgt cagtcctgta cttccgtttt taacgctgcc ttattctcac tccatacatc
     1981 agcaagcttt ctatatcact ttgctcatgt ctgtttatgc tctagctgcc ttcttatctg
     2041 cccccattct cggtagctta agtgatcatt ttgggcgtca tccgatcctt attatcagtt
     2101 tattaggatc aagtatcgga tatttgcttt ttggtttagg aaattcgata tggatgttgt
     2161 tccttggtcg tatcattgaa ggattgacgg caggagaaat ctccacctta tatgcttact
     2221 ttgccgacat cacggaacct aatgaaagaa ccaaagtttt tggatggatg ggtgctttag
     2281 tcggtatagg aacaacgctg ggtcctatta tagggggtct attagctgag ttggggaatt
     2341 ctgttcctat atttattgga gcactcttta ctttcttaaa tgccgtttat ggctacacat
     2401 ttatgctaga aagccttcca ataaaaaaac gttcagtaga tttaagtttc tcccatgtaa
     2461 gaccttttca ccaattacaa caattattca agatttcatc cgttattcct ttattgacag
     2521 caggttttgc tgtttggttg gcagctggct ctcttcaatc tatcttttct caattttcga
     2581 ttgacgcctt tcagtggaag gctgggttgg ttggtttaag cttctctttg attggaatct
     2641 tggatactgt ctcgcaattg ttcattatgc ctcgattgct caaaaaattt tcagaacagc
     2701 agatcacaag aatagggatg ttcagtgaaa tcctcgccta tctattcatc acattatctg
     2761 gaattcttct actgccgatt ttgtttttat ttgggattat ctgttatggt ttcggtgatt
     2821 ctatttttac tcctgtattc aatggccaac tatccaattc tgtctcagat aaccagcaag
     2881 gtcttgtaat gggcggtacc caaagtatac aatctctatc gcgcgtcatc ggcccgctaa
     2941 tcgcaggaca gctttatgct gcagcaccat ttcttccgac ggtattcgga tttttgctta
     3001 tgatttttgc ctattatcac tacaataaaa aatagtagct tcatttgcta cttcgtataa
     3061 attctctgta tcgaccgata aaacgaaata ctgtgtcaaa ttcttctttt gacatatttt
     3121 caaatacagt agcatctcgc tgtacccatt tttgatggag ttgctcgtgt ctattcctta
     3181 ttttttgtcc gattggcgtc aattgaaaat agatttcttt tttattctct tcgctttgat
     3241 aagtgtttat gatccctcta gctatcagtt tttttgttag cttactgatg gctcctctag
     3301 tcatgtaact agcattcgct aattttgtca cgttaggatc agggattttc ccgatatgtt
     3361 caatcacttc tagttcattt agagtaaatc ctgcaaattc tttttccatt tctggtttat
     3421 tacgatgaat aatctcatta gctaaatcca gatattcttc tattagcagt tgtcttttat
     3481 ccatttctac accttctttt tctttcatta tattgttttt ttgtttccta ttcaacaaag
     3541 ataaaaatat tttgtaggaa aacccaatct gaaaccaaga aaaaaagggg ttgacaaagc
     3601 ttgtcataac ccctttttct gattaaaaga tagtaaagag acagtcatcg gtaattgtga
     3661 tgattttttg tcatcactta aggatgagcg gccttttcaa aaactgtctt taaagatcat
     3721 catcagtttc tagcacatat ggctgacctt catatgtgta ctctgtattc aatgtgccgt
     3781 cattttcatc tgagtagatg cgtctactga aaggaaaacg atgagaaaca ttatctttgg
     3841 cgtcatttgc tggcatcgtg taatattcct cttgcgtttc attcaactga gagagtactt
     3901 gatctgccaa ctcatcaata ttgatcgtta atttcttcga agcactttta taatcttcta
     3961 aaaatgcatc ttgttggaca aaatattctt tattcatctt ctttctcacc tttcttttca
     4021 aaatcaaata aagctgcttg ttcttttatg taacagcttt atctttgctg cttattgtga
     4081 caaaaaattc gaagaaagac aactaattgt acttttcaca aaaaaagaca gagggtcgtg
     4141 acaaaattgt gtcacaaccc tttttcttta ttgtgcagtt tcgatttcgc ctagcgctgc
     4201 ttgtgcaagt aggttcaagt aattccatgg gcggtcaaaa tgtggttgga agaagaaatc
     4261 tgaaagtgcg agatcttcta cagtcatttt attttgaaca gctaaagaca tggtatttgc
     4321 tgattgtgta atgtcgtatt tggacatgaa ctgagcacct acgatacgat tcgttccttt
     4381 ttcatagact agctccatca ttactttttc agtagttggc atgaactctg gacgataatt
     4441 atcttcaaat acagtcgctt ggacttctag atcgttcaaa gttgcgcttt ctttggtcac
     4501 acctgtcgaa ccgatcgtcc agccaaatag gtaaagtcct gaagttcctt gagtaccacg
     4561 atacgccatt ttatgatctg tcaagttatt tcccacaagc aaaccttgac gaacggcatt
     4621 tgttgccaaa ggaatatagt tttgcgtgcc acttggattg tagttgacta ctgcagagtc
     4681 tccagctgca aaaatgtctg gattacttgt ctgcatgtat tcattcactt tgattgcccc
     4741 gtttggcagt gtatctactt ttccttcgat cagttttgta tttggacgga agcccacaca
     4801 taaaatgacc atatctgtat caaatgtatc tgttggtgta gctacttgtt tcacttttcc
     4861 ttcttcatct gtgataaatt cagtaacatt ttcgcctagc gcaagagtaa caccgcggtc
     4921 tgtcaattct ttttctaaga tatctgtgaa tggtttatct agatatttat tcaagatgcg
     4981 atctaatcca tcaatcaatg tcacttgttt tccagattcg acaaaagctt ctactaattc
     5041 aataccgata tatccaccac caacaattac tacacgtttt gcttctttcg cttgtgcaat
     5101 aatttcattt gcttgattgt agtttttgca taggagtaca tttttcgcat cgattccact
     5161 aatcggtggg ataataggcc atgaaccagt tgtcatcact aatttatcat aagaaaccgt
     5221 ttctgtttct cctgttttca gatcttttgc cgttactgtt ttattttctg tatcgacgtc
     5281 tgttacatcg tgttccattt taactgttgc tcctaatgaa gataattctt caggatttga
     5341 gtagaacaag cctgctgggt cctttactac tccgccgaca taaagtgcga taccgcaaga
     5401 taagaaagaa atattgtcat ttctttcata tactgtcact tctgcttctg gatgattttt
     5461 taaaatactt tttacagctg cagtacctgc atgagtacag ccaattacta ctactttcat
     5521 ttatggtccc tccgattaac attttgtcct tcgacaaaac tactataaca gaaataaaac
     5581 ggatacaaca gtttaatgct tgtgaattca caaaggtttg tttgtagttt cattcctttt
     5641 caagcataag aacccaatat ctacaagatt cttattttaa taatattcac ttttatatct
     5701 aagattgtga ataaaagaca aaaaaataaa aaagctagcg ttttttttac acagctagct
     5761 ttttttaggt ataacacttg tagaaactgt ttttacattt gcgccttttt tgctgactct
     5821 ttacaatccg gacagagtcc atacaattcc aaccgatgtt cattaatctc aaaatcagtc
     5881 agtctgcttg cagccatttc tatatcttca agccctgggt aatgaaaatc aacaatctta
     5941 ccgcagtttt cacaaattac atgataatga cgctttgaac tgaaatcaaa tcggctagaa
     6001 gcatcgccgt acttcatttc ttgaacaaat ccaatcgaag taaataagcg aaggttattg
     6061 taaacagtcg caacactcat atttggaaac cgatgttcaa gtgctttata aatctcatca
     6121 gccgttggat gggaatgatg ttcaatcaaa tattctaaaa ccgcgtaccg ttgaggtgtg
     6181 attcgaatgt tctctttttt taacttttcg ataacctctt cgactaactt attttccatg
     6241 taaacacccc acgttgttat tctcgtctta tcatactatt tttacatttt aaaatccact
     6301 taacatgaaa agaaaattca attttattgt attttaatga aaaaagacgt aaaacagtcg
     6361 cgttcactat tctacgtctc tattcaaata atttttctca tcataaattt ttgtgtgttc
     6421 ttttcatctt taaaagtttt ttacataaac ctatctggag aaaaaccaag tgccttatat
     6481 ctcttggtaa agggctgtag ataaataacg ttcaccattg tcgggaacca cagctaatac
     6541 cttcttgcct tttcctaatt cttttgcgac ttttagcgcg gcagcaattg cagcgccaga
     6601 tgagatgcct actaaaatcc cttcttttcg cccaacttct ctagctgttt ccatcgcttc
     6661 gtctccacta atagaaagga ctttatcata cacagaagta tccagtgttt taggaacaaa
     6721 acctgtaccg attccttgga ttttgtgagg tcccgcttct ttcccttcta aaatagcaga
     6781 ttctgctggt tctaccccga ttaattcgat tttcggatac acacgtttta attctcggcc
     6841 agcgccagtg atcgttcctc ctgtaccgat acctgcaaca aacgcgtcca agccatttac
     6901 tccaaatgct tgatgtatct caggaccagt cgtcttctca tgcacaagag gatttgcttc
     6961 attttcaaat tgtaatggta aaaagtagcc gttttctttt gccaaacgtt ctgcttctct
     7021 gattgatcca gaaataccat ccgcaccagg agtcaaaatc aactctgcgc cgtacccttt
     7081 catcaataaa cgtctttcga tactcatcgt ctcaggcatc acgatgatta ctttatatcc
     7141 ttttgcaact cctaccatcg acaatccgat tcctgtattt ccagaagtag gttcaatgat
     7201 agtatcacct ggtttcagta gaccatcgtg ttctgctttt tctatcatac ttaaagcaat
     7261 acgatctttt acacttcctc ctggattaaa aaactcaagc tttacaaaaa catctgctga
     7321 atcttctggt acgattttat ttaatttgac gattggcgtt ttgccaatca attcggtgac
     7381 tgagttatag atagctgtca tcttcaattc ctccttgtct ttgctcttct tacttatcat
     7441 aatacaatct gttcatttat ggcgagtgat tcgtctttga ctagcaaaaa aaagacaaag
     7501 atataccata tatggtaatt
//
LOCUS       DAJSBL010000092         7444 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.094, whole genome shotgun sequence.
ACCESSION   DAJSBL010000092 DAJSBL010000000
VERSION     DAJSBL010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7444
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.094"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..1038
                     /locus_tag="ONT85_002631"
     CDS             <1..1038
                     /locus_tag="ONT85_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2379566.1"
                     /translation="IQMKNTIFSDEYVEIIISDHSKNTKEIAKKIEEKKVFDGCHYLE
                     SKSFDDNIGYFQKIKDCLQISCGKSNRYAYYLKNCKSEYFDEFICFSMGEVWLEGIYA
                     CLYRNNKNVQVSILEEGILSYGVDIIPTARTKAIAVLNYLKKRTPLYNKSYNFYCFFP
                     NIYTGRENTIQIPLVTAGSRTALQLSDIFNPMIDIYKEKKYIFFTSVYDFEGGEPVGE
                     YELVCKIADLVGKDNLIVKTHPRDVRTIYKDNGFIEDKNSSIPWEAIQLSGDFNDKIF
                     MTINSGSVISGSTMSAKPVRTYYMYKLCNIEGNPSCKKNAYDIERLLKQKTMKNILKN
                     VKIAESLDDIL"
     gene            1035..2456
                     /locus_tag="ONT85_002632"
     CDS             1035..2456
                     /locus_tag="ONT85_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HCR2379567.1"
                     /translation="MSKTKYLIKNMGILTISNFSSKILVFLLVPLYTSVLSTTEYGTY
                     DLTISTATLLFPILTLNIVDAVMRFLMDKDKNKKNIASIAIKVIFLSVIVFGSVMYLL
                     NITKLWQDVYGLEKYIFLYYISYVWNQFLIQFAKGLEKVKAMGIAGIISTIVTIIANI
                     FFLLILKWGLIGFFIANILSQIISATYLSVLVRIWEYIDFEAKDPQLKREMLNYSIPL
                     IATVVGWWINSTADRYVVSFIVGVSANGLLSVAYKIPQIINTLQGIFIQAWQISAIKE
                     YGDNETAKFYGNTFVMINLLMCITCSSLIILTRPFSHILYAKDFYEAWKYVPFLLIAN
                     VLNCASGLLGPILSAKKDSKSMMWSAIIGALVNVIMSVVLVYKIGVQGATIATVICSY
                     VIYVVRKRAVGKDIFIKHYHIVVESWGLLFVQAIIEIYFKNYVIEIIIMIILIIINIS
                     YIKQLCYRGRNIISMIKSREHKR"
     gene            2492..3208
                     /locus_tag="ONT85_002633"
     CDS             2492..3208
                     /locus_tag="ONT85_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HCR2379568.1"
                     /translation="MVDNYERKNILKKKSIGCRMKIVAIVPMKLNNKRLPQKNTKAFT
                     NGKPLCYYILSTLLKVKQIDKVYVYCSNPDIKEFIPEGVTFLKRSSSLDQDTTSITQV
                     LAGFTKEVEADVYVMTHTTAPFISEKSIEKGLDAVLTGEYDSAFAAKKLQDFLWKDGE
                     PFNYELDNIPRTQDLPPLYEETSGFYIYNRNVMTELHRRIGKKPCIVEVGEIESVDID
                     EAEDFMIADAIYNHLILQGS"
     gene            3227..4810
                     /locus_tag="ONT85_002634"
     CDS             3227..4810
                     /locus_tag="ONT85_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase catalytic domain-containing protein"
                     /protein_id="HCR2379569.1"
                     /translation="MNNVQILDCTLRDGGYCNNWEFGFDNTKKIISGLIESGIEIIEC
                     GFLTNKTVYNKNVTKYTTLEELAQIIPEKREGKLFVAMMNYGEYNIEDLPDYDGSSID
                     GIRIAFHKKNCNEALKLCREIKKKGYLVFVQAMVSLNYTDEEFLAVIKNVNQFKPYAF
                     YIVDSFGMMKGKDLTRLFYMIEHNLEKDIQIGFHSHNNMQLAYSNAQKLITVQTNRSL
                     IIDSSVYGMGRGAGNLNTELFVEYLNDNAGKKYQLKPLLYIIDEILNSFYQKSYWGYS
                     LPNYISAVHNAHPNYASYLSYKNTLTIEAMNEIFEMMDEEKKVSYDKQYIEDLYYKYM
                     ETGKIQEEYMEELRKFLSDKKILLIAPGKSSVDEVERIKEFVTKEEVVIISVNFEYSH
                     IDIDYVFLSNLRRFHELPKESRTKCIVTSNIQASDVYLRTSYKKLLSEREVVRDNAGL
                     MAIRFLADMNVDEIFLAGFDGYSHNEDENYGEQSMEIITKFALLDAMNVQMRDELSEL
                     AKKVKVTFLTDPRWVRIDK"
     gene            4945..5523
                     /locus_tag="ONT85_002635"
     CDS             4945..5523
                     /locus_tag="ONT85_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2379570.1"
                     /translation="MISILCIVAYHANLRLNYNADGIIVHGSLTANQIFLNYIGIWGQ
                     LGVNCFIFISAYFLSEKRNIHFENVLGIVLECIFYSVLVYGILCACGIETFSLNSFVK
                     EILSFALGRYWFITSYLALYILSPYLNIMLNNLEKLQLQKISMLLFIGVCCYRMLFKT
                     SLFGDCAVLIALYVICYTLKKTIIIFLRDTES"
     gene            5469..5987
                     /locus_tag="ONT85_002636"
     CDS             5469..5987
                     /locus_tag="ONT85_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2379571.1"
                     /translation="MLYTEKNDNNIFERHRIIGTIVCITVIPLLISIGILFKFIPNAM
                     VNQMVHIGSPLMVIASIFLFYVFKNFSFYSKYINKLARTSLGVYILHENHYFIDGMKS
                     RGLIWDGIFGLGKRFLDRDFWIWFIIAVVVVCIICMAIDVVREKFERQIFKIKSVKNL
                     CKLMNHVINIYP"
     gene            5995..7035
                     /locus_tag="ONT85_002637"
     CDS             5995..7035
                     /locus_tag="ONT85_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HCR2379572.1"
                     /translation="MRRKEVKVISFGELLLRLAAPGYSKLFQRDSLETTFCGGEANVA
                     VSLAIFGYQSEFITKLPDSDVGYAAANALRYFGVNVDNIIFGEGRMGLYYLEKGAAQR
                     PSKVIYDRKFSAISLADSDEFKWKEILNDANWFHWTGINPALSENLFETSLDACQAAK
                     KRGITISCDLNYRSKLWTSEEAKEKMEQILPLVDICICNEEDAEKVLGIKAEHTNINS
                     GELNKDGYVQVAREIHKKFGCRYVATTLRRSYSASRNGWSAMLYEAEKDKAYFSTEYN
                     IQIVDRVGGGDSFSAGIIYALCENMSGQKAIEFATASSCLKHTIEGDFNRTTIEDVYN
                     LMNSGGNGRVLR"
     gene            7284..>7444
                     /locus_tag="ONT85_002638"
     CDS             7284..>7444
                     /locus_tag="ONT85_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HCR2379573.1"
                     /translation="MTYTYLTTDELVIIESYLKINQSVAKTAHCLNRSSQTIHKVYLF
                     FKQRKSALE"
BASE COUNT         2730 a          850 c         1405 g         2459 t
ORIGIN      
        1 atacaaatga aaaatacaat tttttccgat gagtatgttg aaattattat ttctgatcat
       61 tctaaaaaca ctaaagaaat tgcgaaaaaa atagaagaaa aaaaagtgtt tgatggctgt
      121 cattatttag aaagcaaatc ttttgatgat aatattgggt attttcaaaa gattaaggac
      181 tgtcttcaga tttcttgtgg taaatcaaat cgatatgcct attatttgaa aaactgtaaa
      241 agtgaatatt ttgatgaatt tatttgtttt tcaatgggag aggtttggtt ggaaggaata
      301 tatgcttgct tatatcgaaa taataaaaat gtacaggtat caattttaga agaaggtatt
      361 ttaagctatg gagttgacat tattccaaca gcaagaacaa aagctatcgc agtactcaat
      421 tacttaaaaa aaaggactcc actatataac aaaagctata atttttattg tttttttcct
      481 aatatatata caggaagaga gaatacaata caaattccat tagttacagc tgggtcaaga
      541 accgcattgc aactttcgga tatttttaac cctatgatag atatttataa agaaaaaaaa
      601 tatatatttt ttacgtctgt ttatgatttt gagggcggag aacctgtcgg agaatatgaa
      661 ttagtatgca aaatagcaga cttagtagga aaagataatc taattgttaa gacgcatcct
      721 agagatgtaa gaacaattta taaagataat ggatttatcg aggataaaaa ttcatcaata
      781 ccttgggagg cgattcaatt gtcaggcgat tttaacgata aaatatttat gacaattaat
      841 tcaggaagtg ttatttcagg tagtacaatg tctgcaaaac ctgtacgcac gtattatatg
      901 tataaattgt gtaacataga aggaaatccg tcttgcaaaa agaatgcgta tgatattgag
      961 cgattgctta agcaaaaaac gatgaaaaac attttaaaga atgtgaaaat agcagaaagc
     1021 ttagatgata tactatgagc aaaactaaat atttaattaa aaatatggga atattaacaa
     1081 taagtaattt ttcttcaaaa attttagttt ttttgttagt ccctttatat acaagtgtat
     1141 tatccacaac agaatatggc acatatgatt tgacaatttc aacagccaca ttattgtttc
     1201 caattttaac cttaaacatt gtagatgctg tgatgcgctt tttaatggat aaagataaaa
     1261 ataaaaaaaa tatagcatct atagcgatta aagttatatt tttaagcgtg atagtctttg
     1321 gatccgtaat gtatttgtta aatattacaa aattatggca agatgtttat ggattagaaa
     1381 aatatatttt tttgtattat atatcctatg tatggaatca atttctaata caatttgcta
     1441 aagggttaga aaaagttaaa gctatgggca tagcaggtat tatcagcaca attgtaacaa
     1501 taattgccaa tattttcttc ttgcttattc tgaaatgggg attaattggt tttttcattg
     1561 caaatatttt atcacagatt atttcagcta cttatctgtc agtattagtg agaatatggg
     1621 aatatatcga ttttgaggct aaagatcctc agctaaagag agaaatgcta aattactcga
     1681 ttccattaat tgcaactgtt gttggatggt ggattaatag tacagctgat aggtatgtgg
     1741 tttcctttat agtaggtgtc tcggcgaatg gcttattgtc tgtggcatat aaaatacctc
     1801 aaataattaa tactttgcag ggaatattta tacaggcctg gcaaatttct gcaattaaag
     1861 aatatggaga taatgagact gcaaaatttt atggaaatac atttgttatg attaatttgt
     1921 taatgtgcat aacctgttca agtcttatca tattaactag accgttttca catattcttt
     1981 atgcgaaaga cttttatgaa gcgtggaaat atgttccatt tttattaata gctaatgttt
     2041 taaattgtgc atcaggtctg ttaggaccta tcttatcagc aaaaaaagat tctaaatcca
     2101 tgatgtggtc tgcaatcata ggggcattag ttaatgtgat tatgagtgta gtccttgttt
     2161 ataagatagg ggttcaaggt gccacaatag ccactgttat atgttcatat gtaatatatg
     2221 ttgttcgtaa acgagctgtg ggaaaagata tttttataaa gcattatcat attgttgtgg
     2281 aatcttgggg attattgttt gttcaggcaa tcatagaaat atattttaaa aattatgtaa
     2341 tagaaattat aattatgatt attttaatta ttataaatat cagttatatt aagcagttgt
     2401 gttatagagg aagaaacatt atttcaatga ttaagagtcg agagcacaaa cggtagacga
     2461 tgttattttt ataaaaagat ataatttatt aatggtcgat aattatgaaa ggaaaaatat
     2521 tcttaaaaaa aagagtatag gatgtcgtat gaaaatagtc gctatagtac caatgaagtt
     2581 aaataacaaa agattgccac aaaaaaacac gaaagctttt accaatggaa aaccattgtg
     2641 ttattatata ctttcaacac ttttaaaagt aaaacagatt gataaagtgt atgtctattg
     2701 tagtaaccca gatattaaag aatttatacc agaaggtgta acatttttaa agagatctag
     2761 ttccttagat caggatacaa cgagtattac acaggtatta gccggtttca caaaagaagt
     2821 agaggctgat gtatatgtaa tgacacacac aacagcacca tttattagcg aaaaatctat
     2881 tgaaaaagga ctagatgccg tgctgacagg agaatatgat tctgcatttg ctgcaaaaaa
     2941 attgcaagat ttcttatgga aagacggtga accatttaat tatgaattgg acaatattcc
     3001 tagaacacag gatttgccgc ctttatacga agaaactagt ggattttata tttataatcg
     3061 caatgtgatg accgagttgc atagaagaat tgggaaaaaa ccttgcatag ttgaagtagg
     3121 tgagattgag tcagttgata ttgatgaagc cgaggatttt atgattgctg atgcgattta
     3181 taatcatctt attttacaag gtagttaaga cagggggaag gttaggatga acaatgtaca
     3241 gattttagat tgcacactta gagatggggg ctattgtaac aattgggaat ttggatttga
     3301 taatacaaaa aaaattattt ctggacttat agaatcagga attgagatta tagaatgtgg
     3361 gtttctcaca aataaaacag tatacaataa aaatgttaca aaatatacga ccttagaaga
     3421 attagcacag ataattcctg aaaaaagaga aggaaaatta tttgtagcaa tgatgaacta
     3481 tggcgaatat aatatagagg atcttccaga ttatgatggt tcatctattg atggaattag
     3541 gattgcgttt cataaaaaaa attgcaatga agcgttgaaa ttatgtcgtg agataaagaa
     3601 aaaaggctac ttagtttttg ttcaagctat ggtatctctc aattatacag atgaagagtt
     3661 tttagcagta ataaaaaatg tgaatcagtt taaaccatac gctttttata ttgttgatag
     3721 ttttggaatg atgaaaggaa aagatttaac tcgtcttttt tacatgattg agcataatct
     3781 agaaaaagat atacagatag gttttcactc acataataac atgcagcttg catattctaa
     3841 tgcacaaaaa cttataaccg tacaaacaaa tcgaagcctg attattgatt cttcggtgta
     3901 cggaatggga agaggagcag gtaatcttaa cacagaacta tttgtagaat atttgaatga
     3961 taatgcagga aaaaaatatc aattaaaacc attattatat ataattgatg aaatactaaa
     4021 tagtttctat cagaagagtt attggggata ttcattacca aactatattt cagcagttca
     4081 taatgctcat cctaattatg caagctatct gtcatataag aatactttaa caattgaagc
     4141 aatgaatgaa atattcgaaa tgatggatga agaaaaaaag gtatcttatg ataaacaata
     4201 tatagaagat ttatactaca aatatatgga aactgggaaa atacaggagg aatatatgga
     4261 agaactgaga aaatttctct cagacaaaaa aattctactg attgcaccag gaaaaagttc
     4321 tgtagatgag gtagaaagaa taaaagagtt tgttaccaaa gaagaagtcg taataattag
     4381 tgtaaatttt gaatattctc atatagatat agattacgta tttttaagta atttaagacg
     4441 ttttcatgaa ttaccgaaag aaagcagaac taaatgtatt gttacatcaa atatacaagc
     4501 atcggatgta tatttacgga cgagttataa aaagttgtta tctgaaaggg aagttgtaag
     4561 agacaatgca ggattaatgg caattagatt tttagccgat atgaatgtgg atgaaatatt
     4621 cttagctgga tttgatggat attcacataa tgaagatgag aattatggtg agcaatccat
     4681 ggaaattata acaaaatttg cattattaga tgcaatgaac gtgcaaatga gagatgaatt
     4741 gtcagaatta gctaaaaaag taaaggtgac atttttaacg gatccacgtt gggttcgaat
     4801 tgataagtag tagtaataat gtttttaaaa ttttgataaa gagatagtga gagaataaaa
     4861 atgggtaaag tcttaaataa aaaagaagaa aaatatttaa gggacgatgg ggcgagatga
     4921 aactctaatt ttgaactttt aagaatcatt tctatattgt gtattgttgc ttaccatgct
     4981 aatttgcgtt taaattataa tgcagacgga attatagtac atggttcatt aactgctaac
     5041 cagattttct tgaattatat tggtatttgg ggtcagctag gagttaattg ttttatattt
     5101 atttcggcat attttttaag tgagaaaaga aacattcact ttgaaaatgt tttgggaatt
     5161 gtattagaat gtatttttta tagtgtatta gtttatggaa ttctttgtgc atgtggaata
     5221 gaaacatttt cattgaacag ctttgtaaaa gaaatattat catttgctct aggtagatat
     5281 tggtttatta catcctattt agcattgtat attctaagtc cgtatttaaa tatcatgctt
     5341 aacaatctgg aaaaacttca gttgcaaaaa ataagtatgt tattgtttat aggtgtatgc
     5401 tgttatcgta tgttatttaa aacatctttg tttggggatt gtgctgtttt aattgcacta
     5461 tatgtcatat gttatacact gaaaaaaacg ataataatat ttttgagaga cacagaatca
     5521 taggaacaat tgtatgtata acagttatac cacttctcat atccatagga atacttttca
     5581 aatttatacc aaatgcaatg gttaaccaga tggttcatat tggttctcca ttgatggtta
     5641 tagcaagtat atttttattt tatgtattta aaaatttttc attttacagt aagtatataa
     5701 ataaacttgc gagaaccagt ttaggggtat atattttaca tgaaaatcat tatttcattg
     5761 atggtatgaa gtctagaggc ttaatatggg atgggatatt tggattaggc aaaaggtttt
     5821 tagataggga tttttggatt tggtttataa ttgcagttgt agttgtgtgc ataatttgca
     5881 tggctatcga tgttgtaaga gaaaaatttg aaagacagat ttttaaaatt aagagtgtaa
     5941 agaacctgtg taaacttatg aatcatgtta taaatatata cccataatta ataaatgagg
     6001 agaaaagaag tgaaagttat atcattcgga gaattattat tgagacttgc agcacccggt
     6061 tattcaaagt tatttcaaag agatagtttg gagactacat tctgcggagg ggaagctaat
     6121 gtagcggtat ccttagcaat atttggatat caatcagaat ttataacaaa gcttccggat
     6181 tcagatgttg gatatgcggc agctaatgct ttgcgttatt ttggagtaaa tgttgataat
     6241 attatatttg gagagggacg catgggactg tattatttag aaaaaggagc agctcagaga
     6301 ccatctaaag taatttatga cagaaaattt tctgcaattt cgttagctga tagtgatgaa
     6361 tttaagtgga aggagatcct aaacgatgca aattggtttc attggacagg gataaatcca
     6421 gcattgtctg agaatctctt tgagacgtcc ttagatgcat gtcaggctgc caaaaaaaga
     6481 ggtataacaa tttcttgtga tttaaattat cgatccaagc tttggacatc agaggaagca
     6541 aaagaaaaga tggaacagat actaccattg gttgatattt gtatttgtaa tgaagaagat
     6601 gcagaaaaag tgctaggaat taaagctgag catacaaata ttaatagtgg agaattaaat
     6661 aaagatggct atgtgcaggt ggctagggag attcacaaga agtttggatg taggtatgta
     6721 gctacaactc tgcgtagaag ttattcggca agtagaaatg gttggagtgc aatgttatac
     6781 gaagcagaaa aagataaagc gtatttttcc acagaataca acatacaaat tgttgatcgc
     6841 gttggtggtg gagacagctt ttctgctggt ataatatatg cgttatgtga aaatatgtcc
     6901 ggacaaaagg ctattgaatt tgcgactgca tctagttgtt taaaacatac tatagaaggt
     6961 gattttaatc gaactacaat tgaggatgta tataatttaa tgaatagcgg tggaaatggt
     7021 cgcgtgttgc gttagtcaac atataaagat aaatcacagt aatttttatg gtgattatct
     7081 gtcttagtag tgattagaat aacgtgaaaa taaattgttg agcgtttata ctgttttgaa
     7141 aaataaattt aacatattga aaaatacaaa tatatacgta tattataaag cgccgattgt
     7201 aaaattaagc tagacaacta aaacagtcat ttgtgctaca ctcaaaatag ttccgaccaa
     7261 agaattataa ggagtgagtg caaataacct atacctatct tacaacggat gaacttgtaa
     7321 taatagagtc ttatttgaaa attaaccaat ctgttgctaa aactgcgcat tgcttgaatc
     7381 gttcaagtca aacgatccat aaagtatatc tgttcttcaa gcaaagaaaa tcagccttag
     7441 aata
//
LOCUS       DAJSBL010000093         7393 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.095, whole genome shotgun sequence.
ACCESSION   DAJSBL010000093 DAJSBL010000000
VERSION     DAJSBL010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7393
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.095"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            49..1056
                     /locus_tag="ONT85_002639"
     CDS             49..1056
                     /locus_tag="ONT85_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HCR2379574.1"
                     /translation="MFKGKTILVTGGTGSIGSEIVRQLLKYEPKAIRIYSRGEEKQFY
                     MQQELSNHTNLRFLIGDIRDSQRIDYACRDVDIIFHAAAMKHVPASEYNPMEAVKTNV
                     MGTQNVIDAAIKNNVKHFVGISTDKVVSPTNTMGATKLLSEKLIIAANQYKGKTETIF
                     SCVRFGNVMGSSGSVIPLFSKQIKNNKDITLTDVDMTRFMMSIPDAARLVLSAAMNSK
                     FGETYVLKMPAINILTLAQSLIKIYNDTHDEKYRGEIKVVGIRPGEKLYEELMTTDEI
                     ERSYENDKMYIIPPHYHATDELYKDYEKIKITAYSSDATELMSGQEFIRIAKENNVVT
                     W"
     gene            1068..2072
                     /gene="neuB"
                     /locus_tag="ONT85_002640"
     CDS             1068..2072
                     /gene="neuB"
                     /locus_tag="ONT85_002640"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HCR2379575.1"
                     /translation="MKSEVYVIAEIGVNHNGSLDLALESIDKAKECGANAVKFQTFKT
                     NKLVSKKAPMAKYQKDNLKEDSSQFEMLKKLELSQSDFIRIKDYCIQNKIDFLSTPFD
                     EESADFLKQIGVQGFKIGSGDLTNLPFLRKIDMYGLPVLLSTGMADIHEVSEAVDCFI
                     DSPVTILHCTSNYPASSEDINLLAINTLKEMFGIPVGYSDHSLGYDVGICAVAMGAKV
                     IEKHFTLDKELPGPDHKASLDPQEFADFVKHIRNTEIFLGDGVKRAMPSELSTKEVSR
                     KSLVVTENLNVGDILTEKSLTIKRPGNGIAPKFLYQYIGKKVKRPISADSVLLEEDVE
                     "
     gene            2069..3217
                     /gene="neuC"
                     /locus_tag="ONT85_002641"
     CDS             2069..3217
                     /gene="neuC"
                     /locus_tag="ONT85_002641"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (hydrolyzing)"
                     /protein_id="HCR2379576.1"
                     /translation="MKKILIVSGTRADYGIYKPVAENLLQDNWDVSFVVSGMHLSHKY
                     GFTKELILADGFRISGEVQSLFLENNKGNMARSVALEINGFTNIFEQTSPDFVLLLGD
                     RGEMLAAAIACLYLGIKTAHIHGGEVSGTVDESIRHAITKIASVHFAATEKSKERLIK
                     MGEDSWRVFPVGAPRIDTILNNRLPSFRDIKEKYHLKFEKKKYVLQVFHPVVTELENI
                     EKQILALINSMEQQSQPIICILPNSDAGSEIIRKMYQKYLKTDIIYITNLEPEEYLTI
                     LKECKFMIGNSSSGIIEAASFKIPVINLGTRQNGREQSKNIINANLNENEIETALNKI
                     QDPQFIKSLKNVKNIYGDGNSSRRITKTLNQILNDGEDIKWIQKQIAY"
     gene            3190..3867
                     /locus_tag="ONT85_002642"
     CDS             3190..3867
                     /locus_tag="ONT85_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HCR2379577.1"
                     /translation="MDSKTNSLLKYTSDPYIIIGAGGHAKVIIETLRLENKLIYGFFD
                     DKQNKMQDLKYLGKINELNSWADSKKKIYKFIVAIGDCVVRKKIIKKLNMPSEQYGIA
                     VHPSAIVAKEISIGYGTVIFANAVINPDAVIGEHAIINTGSIVEHDCRINNYVHLSPG
                     VCLAGGVHVGVGTQIGIGSQCIQMKKIGSWCMIGAGSTIVKDIPSHSLAYGNPAKIKK
                     EGINFEF"
     gene            3857..4999
                     /locus_tag="ONT85_002643"
     CDS             3857..4999
                     /locus_tag="ONT85_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HCR2379578.1"
                     /translation="MNSRILLSSPHMGGTEEKYVAKAFKDNWIAPLGENVDKFEESIT
                     KYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATVNPILYQGAVPVFI
                     DSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYERILAIANQFNVPVI
                     EDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSGGGALVSSDKKYVEHSRFLATQ
                     ARDQAPYYQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIFNRYKEAFSGIDRI
                     EMMPEVDGMVPNRWLSTLTINDYNSIDQVVNVINKLNEKNIETRALWKPLHLQPVFQG
                     VKMYYHDNNLSVSEKLFNSGLCLPSGSNMTDSEQEFVIDEFKKIFK"
     gene            5012..5989
                     /locus_tag="ONT85_002644"
     CDS             5012..5989
                     /locus_tag="ONT85_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR2379579.1"
                     /translation="MNIGIIGLGSMGKRRLRLIQEHFNDILLCGVDTNEARRKEVEKE
                     FGIPTYNSLTEAKNLFEINSVFVCTSPITHEFIIKEALNLDCNIFTEINLLNEYYDEI
                     IEVAKKKDRLLYLSSTFLKRKEIQYIKSKVTHNKKISYQYHVGQYLPDWHPWEDYRNF
                     FVSNKKTNGCREIFAIELPWIIDTFGKVKSFTVMTQKLSNLDIDYSDSFTLIIEHETG
                     IVGTLSVDVVSRVAKRNLTIIGEKILIEWEGNPDSLFSWNEETSSMTDIQLYDEVMHD
                     NNYSHTIIEDAYLEEIKEFFTLLEKKMEPQYTFEKDKYIINLINDIEVN"
     gene            5989..6897
                     /locus_tag="ONT85_002645"
     CDS             5989..6897
                     /locus_tag="ONT85_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HCR2379580.1"
                     /translation="MMKVIFFGLGSIGKRHLKNLISYGKKNSINFDITAYSSSGKKEF
                     EEITYIQNRDDLDTHYDIAFITNPTFKHLETLNVIVEKVDYIFLEKPAFSESIDLTQY
                     NYDYKNIYVAAPLRYKNIMEVVRNSIQSKKVYSVRAICSTYLPDWRKGTDYRNNYSAF
                     KSMGGGVELDCIHELDYIINLFGFPKSVKKQFGKYSNLEINSNDLATYILRYDDKIIE
                     IHLDYFGRVPTRKFQLFTLDECINVDLLNNKISFEENIVKEMPESANDMYEKELKYFF
                     ENVVTGKENWNDLYHANEVLKIAEEK"
     gene            6898..>7393
                     /locus_tag="ONT85_002646"
     CDS             6898..>7393
                     /locus_tag="ONT85_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HCR2379581.1"
                     /translation="MEILFTICGRAGSKGFKNKNLKYLAGKPLVHFALAMIDIYQQKH
                     PEHNCTTCLNTDSNELVELTTKLNPSVKIVERKPELATDTVGKIEVIRDSFLQLERED
                     NIFDLIIDLDLTSPLREVSNLEELIDEHLKNLDKDVYFSVVSARRNPYFNMVKRIENE
                     VTIVN"
BASE COUNT         2771 a          912 c         1400 g         2310 t
ORIGIN      
        1 attaagaatc gaattataga tgactataat tcataggagg agatataagt gtttaaagga
       61 aaaacaatat tagtaactgg cggaactggt tccattggaa gtgaaattgt tcgtcagctt
      121 ttaaaatatg aaccaaaagc tatcagaatt tatagtagag gtgaagaaaa acaattttat
      181 atgcaacaag agctctcaaa tcacactaat ttgagatttt taattggtga tatcagagac
      241 tctcaaagga ttgattatgc atgtcgtgat gtggatatta tttttcatgc tgccgcaatg
      301 aaacacgttc cagcaagtga atataatcct atggaagctg taaaaacaaa tgtgatggga
      361 actcaaaatg taatagatgc agcgatcaaa aataatgtaa aacattttgt aggtatcagt
      421 acagataaag tagtgagtcc tactaacacc atgggtgcga caaagttatt gagtgaaaaa
      481 ttaataatag ctgccaatca gtataaagga aaaacagaaa caatattttc ttgtgtaagg
      541 tttggaaatg tcatggggtc aagtggatct gttattcctc tttttagtaa acaaataaaa
      601 aacaataaag atattacttt gacagatgtt gatatgacac gatttatgat gtcaataccg
      661 gatgctgcaa gattagttct gtctgctgct atgaattcaa aatttggaga aacatatgtc
      721 ttaaaaatgc cggcaatcaa tattttaact ttagctcaat cattaataaa aatatataat
      781 gatacacatg atgaaaaata tagaggtgaa ataaaagttg taggtatcag gccaggagaa
      841 aaattatatg aagagctaat gactacagac gaaattgaac gctcatatga aaatgataaa
      901 atgtatatca ttccaccaca ctatcatgct actgacgaac tgtacaaaga ttatgaaaaa
      961 attaaaatca ctgcatactc ttcagatgca acagaattaa tgagtggaca ggaatttatt
     1021 agaatcgcaa aagagaataa tgtcgtgact tggtaaggag ttttatgatg aaatcagaag
     1081 tatatgtaat tgcagaaatt ggtgtaaacc ataatggaag tctagattta gctttagaaa
     1141 gtattgataa agctaaagaa tgtggagcta acgcggttaa atttcaaaca tttaaaacga
     1201 ataagctagt ttccaaaaag gctccaatgg caaaatatca gaaggataat cttaaagagg
     1261 acagttctca atttgaaatg ctaaaaaaat tagaattatc tcaatctgat tttattagaa
     1321 taaaagatta ttgtatacaa aataaaattg attttttgtc tactcctttt gatgaagaaa
     1381 gtgcagactt tctaaaacaa attggtgtac aaggatttaa aattggctca ggagatttaa
     1441 caaatttacc ttttcttaga aaaatagata tgtatgggtt gccagtttta ttatctacag
     1501 gtatggctga tattcatgag gtcagtgagg ctgtagattg tttcatagat agtccagtta
     1561 ctattttaca ttgtacttca aattatccgg ctagttctga agatattaat ttattggcga
     1621 ttaacacatt aaaagaaatg ttcgggatac ccgttgggta ctccgatcat tcattgggat
     1681 atgatgttgg tatttgtgct gttgcgatgg gtgctaaggt aattgagaaa cattttacct
     1741 tggataagga actacctggt ccagatcata aagcttcttt agatcctcag gaatttgcag
     1801 attttgtaaa gcatattaga aacactgaaa tatttttagg tgatggtgtt aagcgagcaa
     1861 tgccttctga acttagtaca aaagaagtat caagaaaaag cctagtggtc actgagaatt
     1921 taaatgtggg agatattctt acagagaaat ctcttacaat aaaacgtcct ggaaatggaa
     1981 tagctcctaa gtttttatat caatatattg gcaaaaaagt taaaagacca ataagtgcag
     2041 atagtgttct gcttgaggaa gatgttgaat gaaaaaaata cttatagtaa gtggaacacg
     2101 agctgattat ggaatatata aacctgttgc tgagaattta ctacaagata attgggatgt
     2161 atcatttgtt gtttctggaa tgcatttatc ccataaatat ggatttacta aagaattgat
     2221 cttagctgac ggatttagaa ttagtggaga agttcaaagt ttgtttttag agaacaataa
     2281 gggaaacatg gctcggtctg tggcattaga gataaatggt ttcacaaata tatttgaaca
     2341 aactagccct gattttgtat tattactagg ggatagagga gaaatgttgg cagctgcgat
     2401 agcttgtttg tatcttggaa tcaaaacagc acatattcac ggaggcgaag tgtctggtac
     2461 tgtagatgaa tcaataagac atgccataac gaaaatagca tctgttcatt ttgctgctac
     2521 ggaaaaaagc aaagagcgtt taataaaaat gggggaagat tcttggagag tttttcccgt
     2581 cggagctcct agaatagata ctattttaaa taatagatta cctagtttta gagatataaa
     2641 agaaaaatat catctaaaat ttgaaaagaa aaaatacgtt ttgcaagtat ttcatccagt
     2701 tgtaaccgaa ctagaaaata tagaaaaaca aattttagct ttgatcaatt caatggaaca
     2761 acaatctcaa ccaattattt gcattttacc taatagtgat gctggtagcg aaattatcag
     2821 aaaaatgtac cagaaatatt taaaaacgga tattatttat attacgaatt tagagccgga
     2881 ggaatacctg actatattaa aagagtgcaa atttatgatc ggaaattctt cttctgggat
     2941 aattgaagct gcatcattca aaataccagt aataaattta ggaaccagac aaaatggacg
     3001 agaacaatct aaaaatatta taaatgcaaa tctaaatgaa aatgaaattg aaactgcttt
     3061 gaataaaata caagatcctc agtttataaa aagcttgaaa aatgttaaga acatctacgg
     3121 tgacggcaac agttctagaa gaataacaaa aacattgaat caaatattga atgatgggga
     3181 agatattaag tggattcaaa aacaaatagc ttattaaaat atactagtga tccatacatt
     3241 attattgggg ctggcggaca cgcaaaagtc attattgaaa ccttaagatt agaaaataag
     3301 ttaatatatg gattttttga tgataagcaa aataaaatgc aagatctgaa atatcttggg
     3361 aaaataaacg aattgaatag ctgggcggat agcaaaaaaa aaatctataa atttattgta
     3421 gctattggtg actgtgtagt aagaaaaaag attataaaaa aattgaatat gccaagtgag
     3481 caatatggaa tagctgttca tccaagtgct atagtggcaa aagaaatttc aattggttat
     3541 ggaactgtta tttttgcgaa tgctgtaatt aacccagatg ctgtaattgg agagcatgct
     3601 ataataaaca caggtagcat tgttgagcat gattgtagaa ttaataacta tgttcattta
     3661 tctccaggtg tatgcttagc gggtggagta catgtaggtg taggaactca aataggaatt
     3721 ggttctcaat gtatccagat gaagaaaatt ggatcttggt gtatgatagg cgcagggagc
     3781 actattgtta aggatattcc ctcacattcc ttagcttatg gcaatccagc taaaattaag
     3841 aaagaaggaa ttaattttga attctagaat tttattatca agtcctcata tgggaggaac
     3901 agaagaaaaa tatgttgcta aggcttttaa agataattgg atagcgccat taggtgaaaa
     3961 tgtggataaa tttgaagaaa gtattacaaa atatactggt tctaagggcg gagcttctgc
     4021 gttaagttca ggtacagctg ccatacatct tgccttaaga ctgcttaatg ttggaaaaaa
     4081 cgatattgtc tttgctcctt catttacttt tatagctaca gtaaatccta ttttatatca
     4141 aggggctgta ccagtgttca ttgactcaga acctaataca tggaatatga gccctgttgc
     4201 actagagagc gcattagaag aagctgaaag aaacaaccaa ttacctaagg caatcatcgt
     4261 agtgaattta tatggtcaaa tggctgatta tgaacgaatt ttagctattg caaaccaatt
     4321 taatgttcca gtgatagaag atgcagcgga atcgttaggt tctacttata agggaatttc
     4381 gtcaggctta tttgggacaa ttgggatcta ttcatttaac ggtaataaga tcatcacaac
     4441 ctcaggtgga ggagctctag tgtctagtga taaaaaatat gttgagcatt caagattttt
     4501 agcaacacaa gcaagagatc aagcaccgta ttatcaacat agcgaaatag gttataatta
     4561 ccgtctaagc aatatttctg ccggtatcgg acgtggacaa atggaagtat tgaatgatag
     4621 agtagctaaa cgaagagaaa ttttcaatcg atataaggaa gctttttctg gaattgatag
     4681 aattgaaatg atgccagaag tggatggcat ggtgccaaat cgttggttgt ctactttaac
     4741 gatcaatgat tataattcta ttgatcaagt agttaacgta ataaataaac taaatgaaaa
     4801 aaacattgaa acaagagctt tgtggaaacc actacatttg caaccagtat ttcaaggggt
     4861 aaaaatgtat taccatgata ataatcttag tgtgtcagaa aagttattta atagcggctt
     4921 atgcttacct tctggaagta atatgacaga tagtgaacaa gaatttgtta ttgatgagtt
     4981 taaaaaaata ttcaagtagg tgtgatattt tatgaacata ggaattattg gtttaggttc
     5041 catggggaaa agaagactta gactcattca agaacacttt aatgatattt tactatgtgg
     5101 tgttgatact aatgaggcga gacgaaagga agttgaaaag gaatttggaa taccaactta
     5161 taatagtcta acagaggcaa aaaatttgtt tgaaataaat tcagtttttg tttgtacatc
     5221 ccctataacc catgaattta ttataaaaga ggctttaaat ttagattgta atatctttac
     5281 ggaaatcaat ttattaaatg aatattatga tgaaataatc gaagtagcta aaaagaaaga
     5341 tagattactt tatctatctt ctacattttt gaagagaaaa gaaattcaat atattaagtc
     5401 taaagtgacg cataataaaa aaatttctta tcaataccat gtaggacagt atttacccga
     5461 ctggcatcct tgggaagatt atcgcaattt ttttgtttca aacaaaaaaa ctaatgggtg
     5521 tagagaaata ttcgctattg aattaccttg gattatagat acatttggga aagttaaatc
     5581 gtttactgta atgacccaga aattatccaa tcttgatatt gattattcgg acagtttcac
     5641 tcttattatt gagcatgaaa ctggtatagt tggaacttta agtgtagatg ttgtttcaag
     5701 agtagctaaa agaaatttaa ctataattgg tgaaaagatt ttaatagaat gggaaggtaa
     5761 cccggattct ctattttcat ggaatgaaga aacttctagt atgacagata ttcaattgta
     5821 cgatgaggtt atgcatgata ataattattc acatactatt attgaagatg catatttaga
     5881 ggagattaaa gaatttttca cgcttttgga aaagaaaatg gaaccccaat atacttttga
     5941 aaaagataag tatattatta acttaattaa cgacattgag gtaaattaat gatgaaagta
     6001 attttttttg ggttagggtc aattggaaag agacatctaa aaaatctaat aagctatgga
     6061 aaaaagaatt ctattaattt tgatataaca gcttatagta gcagtggaaa aaaagagttt
     6121 gaagaaataa cgtatattca aaatagagac gatttggata cacattatga tatagctttt
     6181 attacaaatc cgacttttaa gcatctggaa acactaaatg taattgttga aaaagttgat
     6241 tacatatttt tagaaaaacc tgctttttct gagagtattg atcttactca atataattat
     6301 gattacaaaa atatttatgt tgctgctcca ctgaggtata aaaatatcat ggaagttgtt
     6361 cgtaatagta ttcaaagtaa aaaagtatat tctgtaagag ctatttgttc tacttatctt
     6421 cctgattgga gaaaaggtac tgattataga aataattatt ctgcctttaa atctatgggg
     6481 ggaggagtag aattagactg tatacatgaa ttggattata ttataaacct atttggtttc
     6541 cctaaatcag taaaaaaaca gtttggtaaa tattcgaatc ttgaaattaa cagtaatgat
     6601 ttagcaacat atatattgag atacgatgat aaaattatag aaattcattt agattatttt
     6661 ggaagagttc ctacaagaaa atttcaattg tttactttag atgaatgtat caatgttgac
     6721 ttattaaata ataaaataag ttttgaagaa aacattgtta aagaaatgcc tgaatctgct
     6781 aatgatatgt atgagaagga gttgaaatat ttctttgaaa acgtagtaac aggcaaagaa
     6841 aactggaatg atttatatca tgcaaacgaa gtattgaaaa tagctgagga gaaataaatg
     6901 gaaattttat ttactatttg tggtagagct gggtctaagg ggtttaaaaa taaaaatttg
     6961 aaatatttgg caggaaagcc cctagtccat ttcgctttag caatgataga tatctatcaa
     7021 caaaaacatc ctgaacataa ttgcacgact tgtttgaata cggacagtaa tgagttagta
     7081 gaattgacga ctaagttaaa tccgtctgtg aaaattgtag aacgtaaacc tgaattagct
     7141 acagatactg tagggaaaat tgaagtgata cgtgattcat tcttacaact agaaagagaa
     7201 gataatatat ttgatttaat tatagattta gatttgactt cacctttacg tgaagtttct
     7261 aatcttgaag aattaataga tgaacatcta aaaaatttag ataaagacgt ctacttttca
     7321 gttgtttctg caagacgtaa tccctatttt aatatggtaa aaagaattga aaatgaagtt
     7381 actatagtta ata
//
LOCUS       DAJSBL010000094         6643 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.096, whole genome shotgun sequence.
ACCESSION   DAJSBL010000094 DAJSBL010000000
VERSION     DAJSBL010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6643
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.096"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(1..859)
                     /locus_tag="ONT85_002647"
                     /pseudo
     CDS             complement(1..859)
                     /locus_tag="ONT85_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            1102..1557
                     /gene="tnpA"
                     /locus_tag="ONT85_002648"
     CDS             1102..1557
                     /gene="tnpA"
                     /locus_tag="ONT85_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HCR2379582.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKIFRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            1822..1920
                     /locus_tag="ONT85_002649"
                     /pseudo
     CDS             1822..1920
                     /locus_tag="ONT85_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(2603..3421)
                     /locus_tag="ONT85_002650"
     CDS             complement(2603..3421)
                     /locus_tag="ONT85_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCR2379583.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQ
                     TAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            3582..4271
                     /locus_tag="ONT85_002651"
     CDS             3582..4271
                     /locus_tag="ONT85_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HCR2379584.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            4285..5787
                     /locus_tag="ONT85_002652"
     CDS             4285..5787
                     /locus_tag="ONT85_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCR2379585.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            5800..6276
                     /locus_tag="ONT85_002653"
     CDS             5800..6276
                     /locus_tag="ONT85_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCR2379586.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
     gene            complement(6373..6483)
                     /locus_tag="ONT85_002654"
                     /pseudo
     CDS             complement(6373..6483)
                     /locus_tag="ONT85_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2172 a         1032 c         1204 g         2235 t
ORIGIN      
        1 acttgtcctc gataagaatt gtagcgccgt gatttacgtg tgtatgtcgt tacttttatt
       61 cctagcttct tgatcaggcg ctgaactttc tttttattga tagagaaacc gcggtttctt
      121 agttcattgg tcatccgtaa acagccataa tccttgtgct tctcccgaac tttaagcatt
      181 tccgcttcaa tctcttgatc tggattttta cggtccaatc gtttttgcca gtacataaag
      241 attgccttag ggaactttaa agtttctaga agctcaacta atttaaacgg tcctcggagg
      301 cttgcgatga ttcgtgcgat tgcttcattc gtcgttgttg ggcttcctgt tttcgcagct
      361 tcctcaattc ttttaaaaag gcattttcaa tttgaaggga acggactcgt ttctccaact
      421 cctcgatgcg ttcacgttct ttagacgttg ctttagttgt agattttttc ttatttttgg
      481 gttcattttt cttagacaag ataggtggac atcctttcgt ttttgagagt ccatcgatcc
      541 caccctcacg gaattgacgc atccaactag cgatcaaatt agggttgttc atgttaagtg
      601 tgttggcaac ttcacgatag gacagctcac ttgttagata taactctatc gcatccagct
      661 taaattgaac agaatatttt ttcttttgtc gctttcttac tagaccctct tcaccaaatt
      721 cttgataggc attgatccag cgcttcactt gtgatggagc cttaatggaa tatttctttg
      781 ccagataatc acttccgcct ttaccatcca agtaatcatg aacgatcttt aatttaagtt
      841 caaaagtata cttagccata aaaaacaccg acctccaaaa gttagatttt ttggtctaac
      901 ttttgggggt cggtacaaaa cttaaaagtt ttagaccctt ttattttctt aatgacttca
      961 gtcagttttg tgcgtaaagc gtgcgaaaca agaaaaccgc gcgtttctac gcgtggaaga
     1021 agtatcttta gcgataaaaa ctcctttcga tatactagtg agtggctacc aaccgctcat
     1081 cagatcgaaa ggagtttttt catgtcaaat gacgataaaa gtttagcata tactcgttgg
     1141 aattgtaagt atcatattat ttttacacca aaatatcaac gaaaagtaat ctatgggcaa
     1201 ttacgaaaag atcttggaaa aatatttagg aagttgtgcg aaatgaaaga tgtggaaatc
     1261 atagaagcac atgcaatgct agatcatatt cacatgcttg ttcgagtacc accaaaaata
     1321 tgtatatcca gttttatggg gtatctgaaa ggtcgtagtg cggtactaat ccacgagcag
     1381 cacgcaaatc tcaaatataa atacggaaac aaatcctttt ggtcgaagag atactatgtg
     1441 agtacagtcg gattgaatca aaagacaaat gaaaaataca tccgtgaaca agaagctgat
     1501 gatcgcattc gatatattat aagtaaaaga gagtacgtag aaccatttaa aaaataaaga
     1561 gaagtaagtg agtgaagatg agcagttggc ggtcttttaa aaatcccctt ttaaggggca
     1621 gcgagtgtcg accccttcta gggggattaa aaatacaccc gtcctcacgg gtgtatgggt
     1681 tactgataga cttgatttta actggtgtca gaaacttatg tccagaaagg gtgatgaaat
     1741 tttggcaatt tacggctatt caagaattag cacaagcaac caagattaca aaacacaaat
     1801 tcaaacatta gaaaatgctg gcgcagaaat aatcttccta gaaaatcact ggattatcga
     1861 acacctttgg aagtattttt gagttacgta agtattgatg atctgtctaa cttaatttga
     1921 caattaagag tatatttact tcagatggta aggcaacaaa ataaaataca ataggcagat
     1981 gaactaataa ataaaatctg tattaaatac agataaatta atataaaaat caaaaggatc
     2041 ataaactttt aacaagtgat gaaatgggtg gaaattgatt cttatagaca gtttgtctca
     2101 taacattgaa gtattttgca attttagcat tgaaacactt ttttaatctt caacgttgtt
     2161 cttataaaca agatgattgg gaatcttttg aattaacact gtatagctta gaacaccctt
     2221 tatagaaaac attcgcttag caatcttaat aattccttgt gcccgatgtc gtttagattc
     2281 agtaggttcc tgctcatcga tcatggaaaa aatttgaagg atcagatcct taataaattt
     2341 atttaagaga ggatgatcca ttgcttcatc catactcgat aaactagtga taatgagcta
     2401 cacgtttttc tttcaagttg atcaatagct tccacgataa aaatatcttt agataaaatc
     2461 aagaaccgta ggaactacag ggatatttgg tcaataagag ccaccgctat tagtaaaaaa
     2521 taagctatca agtatgctct catcccaaga attgcctact tcaatcgtta aaaaccgtta
     2581 ggtttagcta agtgggggta gttcactcaa tacttttatc aataatagag cgcaaaactt
     2641 tttctcttaa ttcaactgaa tcaatattat tgttttgttc ataaccatga actaatagat
     2701 cacaaatata aagttgtgat atttttccag ctaaagatcc accattaaga aattcttcaa
     2761 tggctgtttg taaaactaag tctgcagact ttcctatagg tgaatgaata tagttagtta
     2821 tagcaataat ttttgcgcca ttttttttag ctattttcaa tgaatcataa gtatctttcg
     2881 ttttcccaga taaagaaaaa atgattacta aatcattaac tgttaataaa gaagcgactt
     2941 gggcttgaaa atgagggtct agtacagctt ttgcttgaac tcctacgcga agaaacatat
     3001 tttctaaatc aagactagta tttccactag atcccactcc aaatatatat atgttttttg
     3061 attgagtgat cattttaatg gcttcattca atttttcttc gtctaaaagc ctaatagtgg
     3121 aatagagagc ttctgttaaa ctattagcaa tttcatcata gtactttaca ctagtcggat
     3181 gttctttttt ttgactaaag tcttcctttg caatttcaat ttttaaatca gaaaaaccag
     3241 aaaaaccaat tttttgacaa aagcgaataa tagtagcatc gccaactttt gtgtttttct
     3301 taatatcact catcgtacta taaataatag ttttcccaga atttaaaata aaatcagcga
     3361 cttttttttc agacttcgtt aaaaatggat aattttcttt aattaaatag atataactca
     3421 tacaaactcc tctacaattt caacttttct ccattatact ccaaaataat aaaaaaaact
     3481 cctaaatact ccatttttat ctttactttt atgaaagcgg atgctatatt gtttttggag
     3541 ttgttatcca aaaataataa aaaattggag tgtttaggat aatggataaa aagaggtttt
     3601 tagatcttat ttcaaaaggt ttgattgttt cctgtcaagc attacctggt gaaccatttt
     3661 acaaagagga agaaagttta atgccttatt ttgctttggc tgctcaaagg gctggagcag
     3721 ttggtatacg tgcaaattct gttcgagata ttattgaaat tagaaagtgt gtatcattac
     3781 caatgattgg aattattaaa agagattatc caccagaatc tccattcatt actgccacta
     3841 tgagagaagt agatgaatta gtagaaaccg gtgttgaggt aatcgctctt gattgcactc
     3901 ttagaaatcg acatgatgga ctatctataa attcttttat tactaaaata aaagaaaaat
     3961 atcctcatca gttatttatg gcagatattt ctacatttga agaaggcaaa aatgcatata
     4021 aagcaggaat agattttgta ggaactactt taagtggata tactgaggaa agtaaaaaac
     4081 aagatgggcc agatatagat ttgattaaaa aattggtaga agaagggatt tgtgtaattg
     4141 ctgagggaag gattcatact ccagaacaag caaaagagat aaaaaaaata ggagttgcag
     4201 gtattgttgt gggtggtgct attactcgac cacaggaaat tgcatcaaga tttgttaaag
     4261 cattaagtta gggggagaaa taaaatgttt aaaaaatttt cacaattagg acgcgctttc
     4321 atgttaccaa ttgctatatt accagtagcc ggattactat tgggactggg aggagcgtta
     4381 acaaatgaga gtgctataaa tgcttatcca tttttagatc aaccgtggtt gcatacaatt
     4441 ttaagtatta tgagttatgc tggaaatgca gtttttgcaa acctagcact aatttttgca
     4501 attggtattg ctgtaggact tgcaaatgga gataaaggaa cagctggttt agctggtgga
     4561 gtagcttatc tagtatatac tgcaactatt agtgggtttc ttgaactttt ttcagcaaaa
     4621 gatgcaacac tagatacagg tgtagtggga tcaattgttg ttggtggtac tgttgcattt
     4681 ttacacaatc gttatagaaa aattgaatta ccacaattct taggtttttt tggaggatca
     4741 agatttattc cgattgtttc tagtttagct gcaatcatta ttggttcttt cttttattta
     4801 atttggccac caattcaaaa tggtttagta gttgctggtg aacaaattgc ccaaatgggt
     4861 agtttaggaa cttttttata tggtttttta ttgcgtttaa caggtgctgt tggactacac
     4921 cacaccattt accctttgtt ctggtataca tctttaggtg gtaccgaaac tgtagctgga
     4981 tcaacaattg ctggagccca aaatattttc tttgcccaac tagctgatcc aaaccacact
     5041 ggtctgttta cttatggtac tagatttttt gcaggaagat ttgcaacaat gatgtttgga
     5101 ttaccagcag catgttatgc aatgtatcgt gcaattccta agaaaaatcg taaaaagaat
     5161 ggtgggcttt attttagcgg ggcccttaca tcatttttaa ctggaattac tgaaccagtt
     5221 gaatatatgt ttttatttgt tgctccttgg ctttatgtga ttcatgcatt cttagatgga
     5281 ttatcttttt attttgctga tatcttaaat attcgtgtag ggaattcctt ttcaggtggt
     5341 ttgatcgact ttttattatt cggtgtactt caagggaatg acaaaactaa ttggttaaaa
     5401 ttaattcctt ttggtattgc atgggcattt atatatttct ttgtttttac attctttatc
     5461 aaaaaattta aggtagctat tcctggaatg gaagaagaat cagaagttca ttctgaaaaa
     5521 gtaataaaca aaaatacatc attacatgaa gagtctctta tcattattga agctcttggt
     5581 ggaaaagaaa atattgagtc tgttgaagct tgtgcaactc gtttgcgtgt tgctgttacg
     5641 aatggggaga ttattcaaaa acaaacaata aaagacactg gtgcaactgc tgtcttcgaa
     5701 gtgaagggtg gcatccaagc tgtttttgga gggaaagctg acttattaag tcaagaaatc
     5761 aatcaaatat taggaaaaga taattaggag gtttttaata tgtttgaaat tttcaaaaag
     5821 aaaaaacaag aaacttttta tgcaccttgt tccggaaaaa tcatttccat cacagaagta
     5881 aatgatcctg tcttttcaca aaaattacta ggtgatggtt ttgccgtcat acctgatggt
     5941 tcaaaaattt atgctccatt ggctggtcga gtaacaaata tatttcctac taaacatgct
     6001 attaatataa aaagcaactc aggcattgat tatttgattc atattggaat tgatacagtt
     6061 gaattacaag gagagccttt ttcgatttgt gttcaagaaa atgatgaggt tactcctgaa
     6121 acctcgttgg ttgatgtgga ttttgacaaa ataataaaat ctggtaaaga tacttctgtt
     6181 atagttgttt ttccggagaa aaatcagttc aatgatttaa taatagataa tggtcgaatt
     6241 gctcatggga attcatgtgg aaaaatcact ttttaaaaaa gtgtatataa aaagctataa
     6301 aataagctaa ataggatatt tgtcaaatga agttggtgaa cagaaagaac tgttttcttt
     6361 ctgttcattt ttttatttta ttagtaatga ttccccatgc caaatcatga tccgtaattt
     6421 aaagttataa aagcttcgaa atccattggc atttcgttgg aataccttga tatgattatt
     6481 caaatataaa atatagagag aagagagaga gggaagatat ttataaaggg caaagttatg
     6541 tttactcaac ttaggaaata gaaatagatc aatttatatt ctcttgtcaa tcgaatatga
     6601 accttagcac tatataccaa atatagtaaa atgctgaaag atg
//
LOCUS       DAJSBL010000095         6287 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.097, whole genome shotgun sequence.
ACCESSION   DAJSBL010000095 DAJSBL010000000
VERSION     DAJSBL010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6287
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.097"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..177
                     /locus_tag="ONT85_002655"
     CDS             <1..177
                     /locus_tag="ONT85_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2379587.1"
                     /translation="QSLVLLAIVVIGLFLGFKREFSKLIGFLVIALIAVGLVFNASGV
                     KDVLLNLFNRIVGA"
     gene            190..690
                     /locus_tag="ONT85_002656"
     CDS             190..690
                     /locus_tag="ONT85_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HCR2379588.1"
                     /translation="MEQIRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELKGTPIGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCNDMSDVAYYLIVEAGDLGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFELM"
     gene            787..2634
                     /locus_tag="ONT85_002657"
     CDS             787..2634
                     /locus_tag="ONT85_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HCR2379589.1"
                     /translation="MVGNKWYKCDLHIHSTASQCFQDKKVTAKEWVEECLNKGLDCVA
                     LTDHNTGENIDEYKKVAIENGLDFFPGVELTCGENGTHLLVLFDKSACTNDISDFLTK
                     MGIKREDWASAAACSMKSVLEVIDFAEHEGLLVIPAHIDEFNGISSAKFGNLGKIFES
                     ENIYAVQTVQKEFFENRSQMIPSSKRDTIYDSVNARYDGRVGKDTLESWYKSVVEAEK
                     NNMTFLSFSDNPHSKGNSKHGLWGIGTRYSYIKMKDEPDLGSLRDALMLGETRVHSDF
                     SNFSINENEVLLEKLSFSGTTLSTKEVVVEFSDNLTSIIGGRGTGKSCITRFLVYVLG
                     KEAELDQFSEIQSDYQNFAQIEHNGSGIFLKDTIVKLNIFYKGTKYQIIRTQDRHSIY
                     EFTQENGLVEKETERLRMISDKVSIYSQKQIYEISKNQTSILELLDGYNSDLISEYKN
                     EIETCVNEIRKLNWDIVSVKKEIQDKAKVELEIEDLKKQVEKLSHKSYKDVYDEYSKE
                     TDIYRELKRDVEALKEIPKNLTDSVDKIDFGNGIKVTDEIDEHRGELIKNLVTNRAKI
                     QTIINEMSDEIDSYRAELNKSDWKVNYNEVSKRYQRGIKNLVKSYLKMN"
     gene            2637..3533
                     /locus_tag="ONT85_002658"
     CDS             2637..3533
                     /locus_tag="ONT85_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR2379590.1"
                     /translation="MLQNLDSLYQSLSKTEERLEEIIQQEIKLKDYQVEVDSAMSRMN
                     KSYQNLYSERSSSSREIFSDITGIEATIQPNRDFNNYVKELRACINKEDTFDSEFGSL
                     IDKLTKNSITNQDIYNDILDIRVSGESSIFSDKKLINSIRKLNDAQVLELQTLKPNDL
                     VVIRLELNGKKVSLSNASAGQKTSAILSMILAYGNSPLILDQPEDDLDSQLINNLIVR
                     SIITKKENRQILIVTHNANIPVNGDSEWLISMGDSKEISADQCGSVDEPNIKPRICTV
                     MEGGEDAFTNRAKRYGFKQIVE"
     gene            3583..3972
                     /locus_tag="ONT85_002659"
     CDS             3583..3972
                     /locus_tag="ONT85_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HCR2379591.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEEVFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPSTLVRSVNYVSD"
     gene            3959..>6287
                     /locus_tag="ONT85_002660"
     CDS             3959..>6287
                     /locus_tag="ONT85_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCR2379592.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELVPYNYSFLSPEQKFQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLKEVAKQRIDIQTDALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKSLKKSFFSGLQEFVYGVNHHLMGDFVSLSNEEI
                     RCYSKLEKLMESKLARRFKVRRVTPSDLTYLIEHIYGEKGIPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPNRCLIEEKPRCLRMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKGSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEISILNLTSKSENQGLLDPYVIMKKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSDQRGLLQVINELRKDGSPIAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDKQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDQEDQSNQKRLRDLEN"
BASE COUNT         2118 a          933 c         1348 g         1888 t
ORIGIN      
        1 caatctttag tcttactagc gattgtggtc attggtttgt ttttaggatt taaacgcgag
       61 ttttccaaac tgattggctt cttggtgatt gctttgattg cggtaggtct ggtctttaat
      121 gcttctggtg tgaaggatgt cttactcaat ttgtttaacc gaattgtcgg tgcctgaggg
      181 ggaatcacaa tggaacagat acgtgtttat attgcgaacc ttggaaaata caatgaaggt
      241 gagttggtgg gggcatggtt taccccaccc gtcgattttg atgaagtgaa agaacggatt
      301 ggattaaatg acgaatatga agagtacgca attcatgatt atgaactgcc ttttgagatt
      361 gatgagtata caccaattga ggaaattaat cggttgtgtg gtttagcgga agagcttaaa
      421 ggaacgccaa ttggtgaggt cgcttcagag attcaacatg ccttcttcaa ttctttcgag
      481 gaaatggtag agcatgtgga tgatatcatc tattatcccg attgtaatga catgagtgat
      541 gtagcgtatt acttgattgt agaagctggt gatttaggcg aagtaccaga acacttgaag
      601 acgtatattg attttgatgc gtgtggtcga gacttggaga ttggcgggaa ttatttggtt
      661 acgagtcgag gggtttttga actaatgtaa atcatttgtt agaattgacc agaaatttct
      721 atcgtacttt tacttttcta tttgatatac tgaaatagaa aatatttgtt gataggggtg
      781 ttactaatgg ttggcaataa gtggtacaaa tgtgatcttc atattcattc aactgcgagt
      841 cagtgctttc aggataaaaa ggttactgct aaggaatggg tagaagaatg tttaaacaaa
      901 ggactcgatt gtgttgcatt aactgatcac aatacagggg aaaatattga tgaatataag
      961 aaagtggcta tcgaaaatgg cttagatttt tttcctggag tggaattaac gtgtggagaa
     1021 aatggaactc atttactagt cctatttgat aaaagtgcat gtacaaatga tatctctgat
     1081 ttcttaacca agatgggtat aaaaagagag gattgggcca gcgctgcagc ttgctcaatg
     1141 aaatcagttt tagaagttat tgattttgct gagcacgagg gcttgctcgt tattcctgca
     1201 catatagatg agtttaatgg aatttcttca gcaaaatttg gaaatctagg aaagatattt
     1261 gaatcagaaa atatatatgc agtacagacg gttcaaaaag aattttttga aaatcgctca
     1321 caaatgattc cctcatctaa gcgtgacact atctatgata gtgtgaatgc cagatatgat
     1381 ggtagagttg gaaaagatac tttggaatct tggtataaat cagttgttga ggcagaaaaa
     1441 aataatatga catttcttag cttttctgat aacccacact cgaaaggaaa ttcaaaacat
     1501 ggactttggg gaataggcac aagatattcc tatataaaaa tgaaggacga acctgacttg
     1561 ggatcacttc gcgatgcttt aatgctaggc gaaactcgag ttcattctga tttttctaat
     1621 ttctccatta atgaaaatga agttttattg gaaaaattaa gcttttctgg gaccactctt
     1681 agcacaaaag aagttgtagt cgaattcagc gataatttaa cttcaattat tggaggaaga
     1741 ggaacaggca aatcttgtat tacaagattc ttggtctatg tccttggaaa ggaagcagaa
     1801 cttgatcaat tttctgaaat acaaagtgat tatcagaact ttgcacaaat tgaacataat
     1861 ggctctggaa tatttttgaa ggatacaatt gtgaaactga atattttcta taaggggaca
     1921 aaatatcaaa taataagaac acaggataga cattctattt atgaatttac acaagagaat
     1981 ggtctggttg aaaaagaaac agaacggttg agaatgattt ccgataaggt gagtatatac
     2041 tctcaaaaac aaatttatga aatttcaaaa aatcaaacta gtatactaga attactagat
     2101 ggatataact ctgatttgat ttcagagtat aaaaatgaga ttgaaacatg tgtaaacgaa
     2161 ataagaaaat taaattggga tattgtttct gtgaaaaaag aaatacaaga taaggcgaag
     2221 gtagagcttg aaattgaaga tttaaaaaaa caagtagaaa agctatctca taagagttat
     2281 aaagatgttt atgatgagta ttcaaaagag acagatattt atagggaact gaaacgtgat
     2341 gtggaggcac ttaaggaaat tcccaaaaat ctaactgata gtgttgacaa aattgacttt
     2401 ggcaatggta taaaagttac agacgaaatt gatgaacacc gcggagaatt gattaagaat
     2461 ttagtgacca atcgggctaa gattcaaaca ataattaatg aaatgtcaga tgaaattgat
     2521 agttatagag cagagctgaa taaatcagat tggaaagtta actataatga ggtttcaaag
     2581 aggtatcaaa gaggtatcaa aaacttagtg aagagttatc tcaagatgaa ttaaaaatgt
     2641 tacaaaacct tgattcttta tatcaatctt tatcaaaaac tgaagagcga ttggaagaaa
     2701 ttattcaaca agaaataaag cttaaagatt accaagttga ggttgactca gcaatgtcaa
     2761 gaatgaataa atcatatcaa aatctctatt ctgagagaag tagttcctcg cgcgaaattt
     2821 tttctgatat tacaggaata gaggctacta ttcaaccaaa tagggatttc aataattatg
     2881 ttaaagagct cagagcttgt attaataagg aggacacttt tgacagtgag tttggatctc
     2941 ttattgataa gctgacaaaa aattccatta ctaatcaaga tatatataat gacatacttg
     3001 atataagagt tagtggggaa agtagtattt tttcagataa aaaattaatc aattctataa
     3061 gaaaattgaa tgatgcacaa gttcttgaat tacaaaccct taagccaaat gatttagtag
     3121 ttatccgact tgaactaaat ggaaaaaaag ttagtttatc gaatgctagt gctggtcaaa
     3181 agacatctgc aattctttct atgattttgg cgtatggaaa ttcaccactc atattggatc
     3241 agcctgaaga tgatttggat agtcaattaa ttaataactt aatcgtaaga agtatcatta
     3301 caaaaaagga aaacagacaa attttaattg taactcataa tgcaaatatc ccagttaatg
     3361 gtgattcaga atggttgatt agcatgggag attcaaaaga aatatcggct gatcagtgcg
     3421 ggtcggtaga tgaacccaat atcaagccaa gaatatgtac tgtgatggaa ggtggcgagg
     3481 atgcttttac aaatagagcg aaacgctatg gttttaaaca aattgtagaa taacactaga
     3541 gggtgagaca tattattatg tccgcccttt ttaggaggaa aaatgaaaaa aataaaaagc
     3601 tataccagta tctgggcagt tgaaaaagtc atttacgcaa tcaatgattt tcagttgcct
     3661 tttcctgtga cttttaacca aatggcttgg ttcgttttat ctttactgtt tgtcatcgta
     3721 tttgcccatg tgcctccact atctatgata gaggaggtct ttttaaaata cttaggcata
     3781 ccggtggctg tcacttggtt tatgtcgcaa aagacgtttg atggcaagaa accactggga
     3841 tttttacgat cctttatcag ctatcacttg cgatttaagg tgacttttgc aggaaagaaa
     3901 gtcaaagaac agaagaaacg atgggatgaa cccagtactc ttgtaaggag tgtgaactat
     3961 gtatccgatt aagtatattg aaaataatct cgtctttaac caagaaggcg agtgttttgc
     4021 ctactatgaa ttagtgcctt acaattactc ctttttgtca cccgaacaga aatttcaagt
     4081 gcatgataac tttcgccaac taattgcgca aaatcgtgaa ggcaagatcc atgcgttaca
     4141 aattgccaca gaaagtagta ttcgcgccac acaagaacga tcaaaaaaag aaattaccgg
     4201 tagactcaaa gaagtagcga aacaacgaat tgatatacag acagatgcat tagtatccat
     4261 gattggcgat agtcagattg attatcgctt ttttattggc tttaaactaa ttgcgacaga
     4321 tgaagaagtc aatctaaaga gtctcaagaa atctttcttt tctgggcttc aagaatttgt
     4381 ctatggagtg aatcatcatt tgatgggcga ttttgtttcc ttatccaatg aagaaattcg
     4441 ctgctattct aagcttgaaa aattaatgga aagcaaattg gctcgaagat ttaaagtcag
     4501 gcgagtcaca ccgagtgacc tgacgtattt gattgaacat atttatggcg aaaaagggat
     4561 accgtttgaa gaatacgaat ttcagctacc aaagaaaaag ctaaaatcag aaacactggt
     4621 gaaacgttat gacttattac gtccaaatcg ttgtttaatt gaagaaaaac caaggtgctt
     4681 acggatggaa catgagaatc atgagtcgta cgtagcgtat ttaacaatta atacaattgt
     4741 gggggagatg gaatttcctt cctcagaact tttctattac cagcaacaac aatttacatt
     4801 cccaatcgac acaagtatga atgtagagat tgtgacgaat aaaaaggctt tagctaccgt
     4861 tcgtaataag aaaaaagaac tgaaagattt agacaatcat gcttaccaat ctgacaatga
     4921 aacgaattcc aatgtcttag atgctttgga ttccgtggat gaattggaaa ccaccttgga
     4981 tcagtcaaaa gaatctatgt acaaactgag ttatgtggtt cgagtgagtg ccgaatcagt
     5041 ggatgaacta aagcgacgct gtgatgaagt gctcgatttt tatgatgata ccaatgtaaa
     5101 actagtccgg ccctttggcg atatgatggg gctacatgaa gaattcttac cctcaagtaa
     5161 acgatatatg aacgactata ttcagtatgt tacgtctgat tttttggcgg ggcttggttt
     5221 tggggctact cagatgctag gagaattaga agggatttac tttggctaca atgtggatac
     5281 aggtcgtaat gtttacctga aaccggcatt ggcttcacaa ggagtcaagg gttcagtaac
     5341 caatgcgctg gccgccgctt tcctgggttc tcttggtgga ggtaaatcct ttagtaacaa
     5401 tctcttagtc tactatgctg ttttgttcgg tggtcaagct gtgattgtcg atccaaaagg
     5461 ggagcgagga gggtggaaag aaaccttgcc agaaattgcg gatgaaatca gtatcctcaa
     5521 cttgacgagt aagtcagaaa atcaaggtct gcttgatcct tatgtgatta tgaagaaaaa
     5581 gaaagactcg gaaagtttag caattgatat tctgactttt ttaacaggaa tttcaagtcg
     5641 agacggggag aagtttcctg tgttacgtaa agccattcga acggtgactc aaagtgatca
     5701 acgggggtta ttacaagtta ttaacgaatt gcgaaaagac gggtcaccaa tcgcggaaaa
     5761 tatcgccgat catatcgagt cctttaccga ttatgatttt gctcatttgc ttttctcaga
     5821 cggctcgatt acccaatcga ttagtttaga taaacagcta aatatcattc aagtggcgga
     5881 tttggtttta ccggatgcag aaacgacttt tgaagaatat accaccatgg aattgcttag
     5941 tgtttcgatg ctaatcgtca tttccacttt tgccttggac tttatccata gtaaccgaga
     6001 aatcttcaaa attgtcgatt tggatgaggc ttggagtttt cttcaagtgg ctcaaggaaa
     6061 aacgttatcg aataagcttg ttcgtgctgg tcgggccatg aatgctgggg tctatttcgt
     6121 cactcaaaat agcgacgatt tattagatga acgtttaaaa aataatatcg ggttgaagtt
     6181 tgcttttcgc agtaccgata ttcatgaaat caagaaaacc ttggaattct ttggtttgga
     6241 tcaggaggat caaagcaatc aaaaacgcct gcgagatttg gaaaacg
//
LOCUS       DAJSBL010000096         6133 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.098, whole genome shotgun sequence.
ACCESSION   DAJSBL010000096 DAJSBL010000000
VERSION     DAJSBL010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6133
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.098"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(207..1169)
                     /locus_tag="ONT85_002661"
     CDS             complement(207..1169)
                     /locus_tag="ONT85_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004233175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR2379593.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(1171..2610)
                     /locus_tag="ONT85_002662"
     CDS             complement(1171..2610)
                     /locus_tag="ONT85_002662"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HCR2379594.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2795..4741
                     /locus_tag="ONT85_002663"
     CDS             2795..4741
                     /locus_tag="ONT85_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HCR2379595.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKSTFKINGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
     gene            5005..5877
                     /locus_tag="ONT85_002664"
     CDS             5005..5877
                     /locus_tag="ONT85_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HCR2379596.1"
                     /translation="MLYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETMPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKLAWQNFDFIGTMKKEFPIPIS
                     WTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEMGHMLVV
                     PHPKDDFAGSCPFHGNCLEGMAAGPAIEKRLGKKGQEVAEDDPYWEIEASYIAQCAYN
                     TTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGT
                     LGCLALAREAVLHS"
BASE COUNT         1954 a         1056 c         1080 g         2043 t
ORIGIN      
        1 cgtccaattt cgtaaaacaa ccgtaacagt tgtgtaaaaa tatcatttta gagcatgaaa
       61 aaaggagtgg atttctccac tccaaaaaag tctaactttt gggggtcact acaagttgac
      121 aactcctttt tgtttacaaa tcaattggat atctacaaat tgtattttct aacgaatttt
      181 atagacatta aaaaattaat ttaacgttaa atacttcctc cagaaagtat tgagattggt
      241 aaaatataat ccttatttgt cttttcgcct ttaatttttt tcagaagaat atcaacaata
      301 agacttgcaa tttcatcaat tggttgtctt attgttgtca attgtgggaa aaatttctca
      361 ataaaagagg taccatcgta tccaataatt tttaaatctt ctgggatatt aagtttaagt
      421 tgatgagcaa ttttcatagt gagaatggct gttaaatcat cagaaacaaa aataccatct
      481 ggtttgtgat ttataatagt tgactttatt tccatttcac gacggatagt agacagatta
      541 ttaggaattt tgaaaatttt tccatttgga ttttgaaaag aaaaacctag tgcacgtaat
      601 tctgtgggag aatccgtgtt atcatttcct gtaatcataa tggtattttg acaaccattt
      661 ttttgtaaag tcttagctgc aagttttccc ccttcaaagt tatctgaaga aacaatggaa
      721 acattaggag ctaaatttct atcaaaagca acaataggtg ctctcacacg ctcataatca
      781 gcaattccaa gattatggct tgatgagatt atgccatcaa cttggttggc ttcaagcatt
      841 tcaatgtatt ctctctcttt agcaggatca ttttgactat tgcaaataat cgttttatat
      901 ccatgtttaa ataactctat ttcgagatac tcaatgagtt cagagtaaaa gatattacta
      961 atattaggaa aaataagacc aataagctga ggtgactttc cttgaagtcc acgtgctaaa
     1021 ttatttggtt tataaccaag agttttcata gcttcatgaa ctttttgttt tgttttttct
     1081 gataaatatc ccttattatt aatgacgcgt gaaacggttg ttgggctaac tccagctaat
     1141 tcagcgacat cagttaattt tgctaccatt ttaatatttt aattcaaagt attttccagt
     1201 aggttcgcct gatttgattt gaataccatt ttggtgattt tcagggaata cacgactagt
     1261 aaacactttt tctcctttat taataaaaat ctcaatgatt gagttatcta caaaaatgtt
     1321 agcagttgtt tctttagtat caattgagca ttcacgaatc gtaccaaagt cagtggcata
     1381 ttgaatgcct gctttgctgc gatctaaaat aatttttcct tctttggtat ctacaatcaa
     1441 gctaagtcca tttcctttac tatcagagaa aagaagtaac tccgatttct gatttggagg
     1501 aaaagttagt tcaagctcat aagtattatt tgtttctgct tttgctgtaa attgttcaga
     1561 actagcacgt aatgatgtaa tagcttctac tggatattgg taaagtttac catcttttat
     1621 tgaaagttct tttacaagac tcattgctcc ctgataatca tacttatcag ttggataatc
     1681 gatatcaggt agtccaatcc aactgacaat cagagcacga ccatcaggag tattgaatcc
     1741 ttgagtcgca tacgcttcaa atccataatc aagattttga atttctgaag cgcccacaag
     1801 ttttactttt tctatgtcaa gcgattgaca gatcttaaaa gtattcggat agatattccc
     1861 ataatttagc tcagatttat ccaacccttg aggacaataa atgagaacag gttttttgtc
     1921 tacaaatact agatttggac actcaatcat gtattctgaa ccagtgccgc caaaatcaag
     1981 gttgccaact tcctcccatg tttccacgtt attgtcatcc gctttgtaaa gtttgataaa
     2041 gcctgatttt tctaaatttt gtgctccaac aatcgcatac aattgtcctt cataattaaa
     2101 catttgtgga tcacgaaaat gatcagttac atcagcaggt ttatgaataa gaacattctc
     2161 acctttcgta atgttgtagt ctttatccat ccatgctccg atttgaagtg tatcacgttc
     2221 ccaattttca tttcgaacgt ttcctgtata gagtaagaat agattcccct ctatttcata
     2281 tgcagaaccg gaataagcgc cgtggctatc tcgttttgtg tctggaagaa gttctgctcc
     2341 tgttacatga aaatgaacta aatcatctga ttcattgtgt acccattctt tcaacccatg
     2401 agcagctcca aatggccaat tttgataaaa aagcgtatat tttccgttaa aaaaagaaaa
     2461 accattagga tcgtttaaca atcctgtttt aggttcgata tgatagctag cacgccaagg
     2521 tgatcgtttt acattatcct taattttatc catctcatct ttagtccaat cagaataagc
     2581 acgatatcgt atttctcgag gtaagttcat attttactcc ttcaatttat ctttttacaa
     2641 ctagtatggt aaacgttttc cgttgaaaaa tcaatcattt catttaaaaa aataaactat
     2701 atgacaaacg tttgacatat ttttaagata tgctaaaata aatttgcatt tcagtgaacc
     2761 gttttcacgt aaaaataaaa aaggagtgat tcgaatgaat tacagtaaag tagcttccga
     2821 cgtaatacaa gcggtcggaa aagataacct tattgcagct gcacactgcg ctacacgtct
     2881 tcgtctcgta ctaaaagatg acacgaaagt gaatcaaaaa gctttagacg aaaatccaga
     2941 tgtgaaaagc acatttaaaa ttaatggaca atatcaagtg attatcggtg caggtgatgt
     3001 taatttcgtt tatgacgaac ttatcaaaaa aactggactt tcggaaattt caacagatga
     3061 tttaaaacaa attgctagta aagataaaaa attcaacccc attatggcat taattaaatt
     3121 attatcagat atttttgttc caattatacc tgcccttgtt gcaggtggtt tattaatggc
     3181 attacaaaac tttttaacag ctgctggact ttttggacca aaatcaattg aagaaatgta
     3241 tcctgcgatt gaaggtattt cctcaatgat ccaactgatg tccgctgcac cattcatgtt
     3301 cttaccaatt ttagtaggta tttcagctgc taaacgtttt ggagcaaacc aatttcttgg
     3361 tgctgcaatt ggtatgatca tgacaacacc agatcttggt ggctcaactg aatattggaa
     3421 tatttttggt tatcatgttt cacaaacaaa ctatgcttat caagttattc cagttttagc
     3481 agctgtttgg atattatcta tattagaaaa attctttcat aaaaaattgc catcatctat
     3541 tgactttaca ttcacgccat tattatcagt aatgattact ggatttttga ctttcacagt
     3601 tattggccct gtaatgttat tgttgtctaa tggaattaca gacattattg tttggctata
     3661 taacactact ggatttattg gaatgggtat ttttggagga acatattcac ttatagtcat
     3721 gactggttta catcaatcat ttccagctat tgaaactcaa ttactttcag cgtggacaaa
     3781 tggtatcgga catggagact ttatctttgt agttgcatct atggcaaacg ttgctcaagg
     3841 tgctgcaaca tttgccattt ggttcttaac taaaaattct aagacaaaaa gtcttgcttc
     3901 ctctgcgggt gtctcagcac ttcttggaat tacagagcct gccttgttcg gagttaactt
     3961 aaaataccgc tttccattct tttgtgcttt aattggttca ggaattgcta cagcagtagc
     4021 aggattactt aaagtagttg ctgtttcact tggttcagca ggtttcttgg gatttctttc
     4081 aattaatgca acatctattc cattttacgt attgtgtgaa ttgattagct ttgtagttgc
     4141 ttttgcaatt acttacttct acggtaaaac aaaagctgct ggtatcttta aagcagaaga
     4201 aattgctgaa caaacaccag ttaattcttc aaaagtagca acaacagatc caatatctac
     4261 tgatgttgaa tcaacacaaa caaaagtttc atcagaaaca attgttagtc ccatttctgg
     4321 taaagtgatt agtctttctt cagtaaatga tccagtcttt tcatcagaag ctatgggaaa
     4381 agggattgcc attaaaccaa atggaaacac ggtccattct ccagtagatg gtactattca
     4441 actcacgttt gaaactggac atgcttatgg tattaaatca aataatggtg ctgaagttct
     4501 aattcatatt ggtattgata ctgtatcaat gggaggaaaa ggatttacac aaaaagttac
     4561 tactaatcaa aaagttagaa aaggcgatat tcttggagca tttgaccgtt caatgattga
     4621 gaaagaaggt cttgatgata caacaatgat tattgtgacc aatacttcgg attattcaga
     4681 agtaaatata gtagctgaaa atacagtcac tgagggtact gatttacttg aaatcaaata
     4741 aatatatttc cctgagtaaa gatggaattt tctttccaaa atcaaataat atagattaat
     4801 caaatatttg agaataacat tttatttaat cttttcatat atcaattgta tgaaaaatta
     4861 ttagaatttt aaaaaatgat aaaaattcaa aaagaaaaaa tagtagttat ttacttgtca
     4921 tgttactaaa taaaaaaata atttaaatat gatatatagt catttattta gtgatttcat
     4981 tctaaaataa agggagaaat gtctatgcta tacggtgcaa ttgaagcagg cggcacaaaa
     5041 tttgtgtgtg ccattggcga tgaagaaatg acgatcaagg aaagagtgag ctttccgaca
     5101 acgacacctg aggaaacaat gcctttagtc attgacttct tcaaacaata ccaagcagat
     5161 ttagcaggga ttgggattgg ctcatttggg cctatcgata tccatcgcga ttccgccact
     5221 tatgggtaca ttacctcgac cccgaaattg gcatggcaaa attttgattt catcggtacc
     5281 atgaaaaaag aatttccgat cccgatttct tggacaacag atgtcaatgc ggctgcctat
     5341 ggtgagtatg tcttcggcag tggcaaagga ttgagcagtg tcgtttacta tacgatcggt
     5401 acgggtgttg gcggcggtgc cttacaagaa gggcgcttta tcgaagggtt tagtcatcca
     5461 gaaatggggc atatgctcgt cgtccctcat ccaaaagatg actttgctgg cagctgtcca
     5521 tttcatggca actgtttaga agggatggcc gctggacccg ccatcgaaaa acgcttaggg
     5581 aaaaaaggac aagaggttgc agaagatgat ccttattggg aaatcgaagc ctcctatatt
     5641 gctcaatgtg cctacaatac aaccttgatg ttttctccag atgtgatcat ttttggcggt
     5701 ggtgtgatga aacaacgcca tatgctgcaa aacgtccacg acgcatttgc aaaattggtc
     5761 aatgggtatg tcgaaacacc tgagttaagc aaatatatcg tgaccccagc attagaagac
     5821 aacgctggaa cgctcggttg tttggcattg gctcgagaag cggtcttaca ttcataaacg
     5881 aaaaaaggct cttcgacatt tagtgttggt agagaaagat acaactgaaa atttagtcat
     5941 aaccagttag gcagagtgct taactggttt ttgttttact tggaagatca agccttgaat
     6001 gagataaatt cttgctcgaa agttatggaa atttcgatag ccgtaggcca cttgtattgt
     6061 gcaatagttt tttacacgcc gtttgtataa ttaagcgaga caaataaaaa agccatttat
     6121 gttacactca aat
//
LOCUS       DAJSBL010000097         6002 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.099, whole genome shotgun sequence.
ACCESSION   DAJSBL010000097 DAJSBL010000000
VERSION     DAJSBL010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6002
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.099"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..395
                     /locus_tag="ONT85_002665"
     CDS             <1..395
                     /locus_tag="ONT85_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2379597.1"
                     /translation="ISLKDESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQE
                     YYFYKCITKRVCCICGKTGADIDHFDKALGRRKRKEVDHSEYTFAALCRIHHTEKHKI
                     GVINFKNKYQIKGIKLSHETIKKLRIGG"
     gene            398..1228
                     /locus_tag="ONT85_002666"
     CDS             398..1228
                     /locus_tag="ONT85_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer N-terminal
                     domain-containing protein"
                     /protein_id="HCR2379598.1"
                     /translation="MAEISWIKLKTTMFDDEKIRLIQAVPESDAIIVIWIRLLVLAGK
                     TNDDGLIYIQRNMPYTEEMLATLFGKNVNTVRLALTTLANFNMIDLSSDGLIAISNWE
                     KHQNIEGMDKVRLKNAERNRKYRERKRQERLKLENDVSVTSRDGTDKDIEEDKDIDKE
                     EKKGKYSDEHLRLAKKMQRNLTEDFPKEMNKVDIEKWADTIRLMEERDKASIEAIEYV
                     INWLPTNEFWFGNIRSAKKLREKFEKLKFEIKADKKNHKKQSQKLQYSNPSEYDDLPI
                     "
     gene            1244..2095
                     /locus_tag="ONT85_002667"
     CDS             1244..2095
                     /locus_tag="ONT85_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCR2379599.1"
                     /translation="MESLANAMEKLIRRVLVQSGKCPECSEPLYSWRAKNKDGSERCK
                     PTCMSCGYKALRVKEDIQTERIYNDSLKARALSFFQNGSVLTDKTLFKCKMENYHVVD
                     QETKIALERAKSYVNDVLLNHPAHFILSGKSGSGKSHLSMATAWEILERSNYDKKILF
                     ISYQELLEQIKFSYNNAELRKEIEGSLIADIKTTDLVVFDDIGAELGSGVSNSRQFTN
                     NTLNTLLEARQNKATIITTNLSGPELREAYGERIVSRIFKNSEGYALKFQQTADKRIK
                     PVKGSIA"
     gene            2092..2451
                     /locus_tag="ONT85_002668"
     CDS             2092..2451
                     /locus_tag="ONT85_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HCR2379600.1"
                     /translation="MNKYRNKKTVHRGIKFDSIAEAEYYDLALWQAEANGWKVKLQER
                     FELMPKFELDGKKYRKIEYIPDFTFYKNGKLVKVVDVKGMQTKDFKIKAKLFCSQYRV
                     PLILAKKYRNTFKEERF"
     gene            2466..2627
                     /locus_tag="ONT85_002669"
                     /pseudo
     CDS             2466..2627
                     /locus_tag="ONT85_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
     gene            2624..2929
                     /locus_tag="ONT85_002670"
     CDS             2624..2929
                     /locus_tag="ONT85_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2379601.1"
                     /translation="MSVDKKAAMKRIIELTHSENWQEDKEIVAEVQKLGKSMWAEKPK
                     RKTPRKIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEK"
     gene            2929..3240
                     /locus_tag="ONT85_002671"
     CDS             2929..3240
                     /locus_tag="ONT85_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HCR2379602.1"
                     /translation="MNELITKVEQWAKDKGLDQADPKAQFLKVAEEFGEIASAMARSN
                     DELFKDSVGDVIVTLIILSMQKGANIQECLEMAYNEIKGRTGKMVDGVFVKSSDLEDS
                     E"
     gene            3237..3410
                     /locus_tag="ONT85_002672"
     CDS             3237..3410
                     /locus_tag="ONT85_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2379603.1"
                     /translation="MMMPKEIYSDALRGARNQLKMAKRAYEIRPTIENERRVKAIRRR
                     CSIYGELQKEDSK"
     gene            3407..3823
                     /locus_tag="ONT85_002673"
     CDS             3407..3823
                     /locus_tag="ONT85_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YopX family protein"
                     /protein_id="HCR2379604.1"
                     /translation="MIPRFRAWDKRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIELMQSTGLKDKNGIEIFEWDIVSVSVRNGFDYLDNKVCVVKNSIGHSGLVCAT
                     VDEDLEYQIFNTELFEEYTYEVIGNIWENSELLEEQ"
     gene            3823..4491
                     /locus_tag="ONT85_002674"
     CDS             3823..4491
                     /locus_tag="ONT85_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HCR2379605.1"
                     /translation="MNKQELIDELAKYVKRYENVMDEHGQGRYGAYEVSLKLVKRLNE
                     SKITDEQAWNKVAEAYPESAQSLRNTLDNAVFGKTGEPQKHVMPKFVAEWIEYAKKKG
                     DSLAISFKPWNLYGVEYSKADRWIGDNQETFARAWLDGYEVEKEPLYYVPLPYEVWDE
                     EAAELKTEYLYLHYEITSDETRIFPTKEPRKGFVAKLDELTIKSADENYWPFAVPVEE
                     VVEG"
     gene            4488..4865
                     /locus_tag="ONT85_002675"
     CDS             4488..4865
                     /locus_tag="ONT85_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2379606.1"
                     /translation="MKKLHQLISEKESELQNLEDSLGLGFPIVEQVKMVQISHLQLEL
                     EDLRQIEDPYQLNDNQQIVLEWLKLTASTGKPMQVVFWMMNNAAWGHLDELRDPLMEL
                     TDKQQFEVLTAFAQWGLEQEEKE"
     gene            4862..5161
                     /locus_tag="ONT85_002676"
     CDS             4862..5161
                     /locus_tag="ONT85_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2379607.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKPKVKVGDMVRCEA
                     EGFIYPFRGYVEHVYNHSAIIRIENTMECDKLLAKSKDNLAVVRLVDMEVINNEI"
     gene            5151..5309
                     /locus_tag="ONT85_002677"
     CDS             5151..5309
                     /locus_tag="ONT85_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2379608.1"
                     /translation="MKFKIFEEDTRYKLEKKLNEFAKNNEIQHISLTASKSGYTTYYA
                     AVVSYVIQ"
     gene            5403..5819
                     /locus_tag="ONT85_002678"
     CDS             5403..5819
                     /locus_tag="ONT85_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HCR2379609.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCDVNKHSNYEI
                     GQLIRGYGEKNVEKLKSIALIEFAEAYKKGVLVQYR"
BASE COUNT         2231 a          899 c         1391 g         1481 t
ORIGIN      
        1 aaataagcct caaagatgaa tcgaatacga ctgtgagtga tgctaatgtc ttagcaaata
       61 tcatcttaga tttcatcttt gaacatcata ttcctttcaa agaaggctat gagattttac
      121 cagcgaatca agaatattac ttctacaaat gcattacaaa aagagtttgt tgcatctgtg
      181 gcaaaacagg agctgacatc gatcactttg acaaagcgct aggaagacga aagcgaaaag
      241 aagttgatca ttcagagtac acatttgcag cactctgcag aatccatcac acggagaagc
      301 acaaaatagg tgtgatcaat tttaaaaata aatatcaaat caaaggaatc aagttaagtc
      361 atgaaacgat taaaaagtta aggataggag gataaatttg gctgagataa gttggatcaa
      421 acttaaaact actatgtttg acgatgaaaa aatacggtta atccaagctg ttcctgagtc
      481 ggatgccatc atcgttatat ggattcgatt actagtttta gcaggaaaga ctaacgacga
      541 tggtctgata tatatccaga ggaacatgcc ttataccgaa gaaatgcttg ctacattgtt
      601 tggcaaaaac gtaaatacgg ttcgcttagc gctaactaca ttggcaaatt tcaacatgat
      661 tgatctaagc agtgatggac taattgccat cagtaattgg gaaaaacatc aaaatatcga
      721 gggtatggat aaagtaaggc taaaaaatgc tgaaagaaac cgtaaataca gggaaagaaa
      781 gagacaggaa cgtctcaaat tggaaaatga cgttagcgtg acgtcacgtg acggtacaga
      841 taaagatata gaagaagata aagatataga taaagaagaa aagaaaggta agtattctga
      901 cgaacactta cgccttgcta aaaaaatgca aaggaattta actgaagatt ttccaaaaga
      961 aatgaacaaa gtagatatcg aaaaatgggc agacacaatc aggttgatgg aagaaagaga
     1021 taaagcatct atagaagcga ttgagtatgt gatcaattgg ctacctacaa atgaattttg
     1081 gtttggaaat attagaagtg ctaagaaatt gagagaaaaa tttgagaagc tcaaattcga
     1141 aatcaaagca gacaagaaga atcataaaaa gcaaagtcaa aaactacagt acagcaatcc
     1201 tagtgaatat gacgacttgc caatttaaaa ggagatgcat cacatggaaa gcctagcaaa
     1261 tgctatggag aagctaataa gaagagtatt agtgcaaagc ggaaaatgtc cagaatgtag
     1321 cgaacctttg tatagttggc gagctaaaaa taaggatggt tcagaacgtt gtaaaccaac
     1381 atgtatgagt tgtggttata aagcgttacg tgtgaaagag gatatacaga ccgaacggat
     1441 atataacgac agcttaaaag cacgagcatt gagttttttt caaaatggtt cggtattaac
     1501 agataaaact ttgtttaaat gcaaaatgga gaattatcac gtagtggacc aagaaacgaa
     1561 aattgcttta gaaagagcta aaagctatgt aaatgatgtc ctactgaacc atcctgcaca
     1621 tttcattcta tcagggaaat caggaagcgg aaaaagccac ttatcaatgg cgacagcttg
     1681 ggaaatactt gagcgctcaa attatgacaa gaaaatactt tttataagct atcaagagtt
     1741 attagagcaa ataaagtttt cttataacaa tgctgaactg agaaaagaaa ttgaaggatc
     1801 gcttatagcc gatatcaaaa caactgattt ggtggttttt gacgatattg gagctgaatt
     1861 aggtagcggg gtatcaaata gtaggcaatt tacaaacaac acgttaaaca cgctcttaga
     1921 agccagacag aacaaggcaa cgatcatcac aacaaactta tctggtcctg aactaagaga
     1981 agcctacggt gaaagaattg tttctaggat atttaagaat tcagaaggtt atgcgctgaa
     2041 attccaacaa acagcagaca agcgcataaa accagtgaaa ggtagtatcg catgaataaa
     2101 taccgtaata aaaaaactgt tcatcgaggt atcaagtttg attctatcgc agaagcagag
     2161 tattacgatc tagccttgtg gcaagctgaa gcgaacggct ggaaagtaaa acttcaggaa
     2221 agatttgagc tgatgccgaa atttgaacta gacggaaaga agtatcgcaa gatcgagtat
     2281 attcccgact tcacatttta taaaaacggc aaactagtca aagtcgtaga tgttaaaggg
     2341 atgcagacaa aagatttcaa aatcaaagcg aaactcttct gcagtcaata tcgagtacca
     2401 ctgatcttag cgaagaaata tcggaatacg ttcaaggaag aacggtttta acgaggtgat
     2461 ccatcatgac aacagaagaa gtgattcaaa tgcgtattcg aagccttcag cgtgagattg
     2521 acgatctgta acggacaaag gcagtgatgg tcaatgaaac ggcgaggaag gcaatcgatt
     2581 tgcacataga aaatttaaga aggaaaatcc atcgattgga ggaatgagcg tggataagaa
     2641 agcagcaatg aaacgaatca tcgaactgac acattctgag aattggcaag aagacaaaga
     2701 aatagttgca gaagtccaaa agctcggtaa atcaatgtgg gctgaaaagc ctaaacggaa
     2761 aacgccgaga aaaattgcaa tctggcatgg tgatcgaatt ctagtaacag gtactgctga
     2821 acagttatct gaaattactg gattaagcaa aaacattatc tgggatagag ctaggagctt
     2881 atggattgat tcaaaaggac gacagtttag gtatgtggag gagaaataat gaacgaacta
     2941 atcacaaaag tagagcagtg ggctaaagac aagggactgg atcaagcgga tccaaaagca
     3001 caatttttga aagtagctga ggaattcgga gaaattgcat cggcaatggc aagaagtaat
     3061 gatgagctat ttaaagatag cgtaggagac gttatcgtca cgctgattat tctttccatg
     3121 caaaaagggg caaacataca agagtgttta gaaatggcgt ataacgaaat caaaggacgc
     3181 acaggaaaaa tggtagatgg tgtattcgtg aagtcgagtg atttggagga cagcgaatga
     3241 tgatgccaaa agaaatctat tcagatgcat taagaggtgc tagaaatcaa ttgaaaatgg
     3301 caaaaagagc atacgaaatt cggcccacaa tcgaaaatga acgcagagta aaagcaattc
     3361 gccgtagatg ttcaatttac ggcgagttac agaaggagga cagcaaatga taccgagatt
     3421 tcgagcttgg gataaacgaa agaacgtaat gagagatgta gccgtcttgc attttactaa
     3481 aaacggcaaa acaaacttta ttgaatattg gataaatcct accgaattga aatcatatca
     3541 cgtgcgaaac atcgagctca tgcaatcaac agggctgaaa gataagaatg gcattgagat
     3601 tttcgagtgg gatattgtat cagtcagcgt gcgaaatgga ttcgattatt tagataataa
     3661 agtttgtgtt gtcaaaaatt caataggaca ttcgggatta gtgtgtgcca ctgttgatga
     3721 agatttagag tatcaaattt ttaacacaga gctgtttgaa gaatacacgt atgaagtcat
     3781 cggaaatatt tgggagaata gcgagttatt ggaggaacag tgatgaataa acaggaattg
     3841 attgatgaat tagctaaata tgtaaagcgt tatgagaacg ttatggatga gcatggccaa
     3901 ggaaggtacg gcgcttatga agtatcttta aagttggtga aaagactaaa tgaatcaaaa
     3961 attacggacg aacaagcttg gaataaggta gctgaggctt atcctgaatc ggcacaaagc
     4021 ttgagaaaca ctttagataa tgctgtattt ggtaagactg gtgaaccgca gaagcacgtt
     4081 atgccgaagt ttgtggcgga gtggatagaa tacgctaaaa agaaaggtga tagtctagct
     4141 atttcattca agccgtggaa cctctatggt gttgagtata gcaaggctga tagatggatt
     4201 ggagataatc aagaaacgtt tgctcgtgct tggctagacg gctacgaggt cgagaaagag
     4261 ccgttgtatt atgtcccgct accatatgaa gtttgggatg aagaggcagc ggagcttaaa
     4321 acagagtatt tgtatctaca ttatgagatc acaagtgatg aaactagaat atttcctaca
     4381 aaagaaccta gaaaaggttt tgtcgcaaaa ttagatgaac ttacgattaa atcagcagat
     4441 gaaaactact ggccgtttgc tgtaccagtg gaagaggtgg tagaaggatg aaaaaattgc
     4501 accaactcat atctgagaaa gaaagcgaac tccaaaattt agaagatagt ttgggattgg
     4561 ggtttccaat tgttgagcaa gttaaaatgg ttcaaatcag tcatctacaa cttgaattgg
     4621 aagatttaag acaaatagaa gatccgtatc aactcaacga taaccagcaa atcgtgttgg
     4681 agtggttgaa actaactgcg tcaacaggaa agccaatgca agttgtattt tggatgatga
     4741 ataatgcagc atgggggcat ttagacgaat tgagggatcc tttgatggaa ttaactgaca
     4801 aacagcaatt cgaagtccta acagcattcg ctcaatgggg attagaacag gaggaaaaag
     4861 aatgaaacta aaagacggat tttacgctag tagccacggt atcggcggtt taatgctaga
     4921 catgccgaca aagaacccta aaacacgtaa gaagccaaaa gtcaaagtcg gtgacatggt
     4981 tcgctgtgaa gcagaaggat tcatctatcc atttcgtgga tatgtagaac acgtctataa
     5041 tcactcagcg atcattcgca ttgaaaacac gatggaatgc gacaagttat tagcgaaaag
     5101 caaagataat ttagcagtgg tgagattggt ggatatggag gttataaaca atgaaattta
     5161 aaatctttga agaggacact cgctataaat tagaaaaaaa attaaatgaa tttgcaaaga
     5221 ataatgaaat tcagcatata tctttaacgg cttctaagtc aggttataca acttactatg
     5281 cagctgttgt gagctacgta attcaataaa aggaactcgg caaataaaaa agccggatca
     5341 ctccgactga tgtaataaat ccgacaagtt tattatatca cataaaggag cggtttgact
     5401 tgatgcaatt gttacgagag gtagatttca aacagacaag atgtaatgcg agagatgtgc
     5461 tgaagaactt tcggcgcttg gagcggatgg caggtcgctc tttgatagat attaagtcgc
     5521 cgattattac ggatatgccg aaggcaccga agcacggcaa taaggcagaa gacgcgatca
     5581 ttcagatgat ggatatagaa gcggagagag acgcgatttt agcggctttg atggcgctta
     5641 gtctgattag ccgtcagata ctctactaca gcttctgtga cgtaaacaag cactctaatt
     5701 atgaaatagg gcaattgata cgaggatacg gagagaagaa tgtagagaag ctgaaatcca
     5761 tcgcattgat cgaatttgct gaagcataca aaaaaggcgt gttagttcag tatcgttaat
     5821 tttgtagggt ttttgtaggg atagtgtagg gtttttgagc ggtttaacgt gatattatga
     5881 tagtgtcgaa agattagtga taggtctaag acaaaataat aataaaagga acatcgtttt
     5941 attattgttt cacaattaag cttcgataga cagcagcgga aatattaaga ataaggatgt
     6001 ga
//
LOCUS       DAJSBL010000098         5793 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.101, whole genome shotgun sequence.
ACCESSION   DAJSBL010000098 DAJSBL010000000
VERSION     DAJSBL010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5793
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.101"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..988)
                     /locus_tag="ONT85_002679"
     CDS             complement(<1..988)
                     /locus_tag="ONT85_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS1542 family transposase"
                     /protein_id="HCR2379610.1"
                     /translation="MTQVHFTLNNEEVQSIIEHSVKDDVSKNILTTVFNQLMENQRTE
                     YIQADDYERSESRQSQRNGYYERDFTTRVGTLELKVPRTRDGEFSPTVFERYQRNEKA
                     LLASMLEMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQLDPMVNEWQNRSLSGTN
                     YPYLMTDVLYIKVREDHRVLSKSCHIAIGITEGGDREIIGFMIQNEESDDTWSIFFEY
                     LKERGLKGTELIISDAHKGLVSAIRKSFTNASWQRCQVHFLRNIFSSIPKKNSKPFRE
                     AVKAIFKFTDIELARTAKNALVGEYIDQPKYTKACEILDNGFEDAFQYTVIGN"
     gene            1140..1835
                     /gene="vanR"
                     /locus_tag="ONT85_002680"
     CDS             1140..1835
                     /gene="vanR"
                     /locus_tag="ONT85_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HCR2379611.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            1813..2967
                     /locus_tag="ONT85_002681"
     CDS             1813..2967
                     /locus_tag="ONT85_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HCR2379612.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
     gene            3182..4150
                     /locus_tag="ONT85_002682"
     CDS             3182..4150
                     /locus_tag="ONT85_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HCR2379613.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            4143..5174
                     /gene="vanA"
                     /locus_tag="ONT85_002683"
     CDS             4143..5174
                     /gene="vanA"
                     /locus_tag="ONT85_002683"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HCR2379614.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            5180..5788
                     /locus_tag="ONT85_002684"
     CDS             5180..5788
                     /locus_tag="ONT85_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HCR2379615.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPKRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT         1745 a         1131 c         1310 g         1607 t
ORIGIN      
        1 tatttccgat aaccgtgtat tgaaaggcat cttcgaagcc attatccaat atttcgcagg
       61 cttttgtata tttaggctgg tcgatatatt caccgactaa ggcattctta gctgttcgag
      121 cgagttcaat atccgtaaac ttaaagatcg cttttactgc ttctctaaac ggttttgaat
      181 tcttttttgg aatggaactg aagatgtttc ttaaaaaatg gacctggcat ctctgccaac
      241 ttgcgttggt aaatgactta cgaatcgcag acactaggcc tttatgggca tcagaaatga
      301 tgagttctgt cccctttagg ccgcgttctt ttaagtattc aaagaagatg gaccatgtgt
      361 catcactttc ttcattttga atcatgaagc caatgatttc acggtcgcca ccttctgtta
      421 tcccgatcgc aatatggcag cttttagaaa gcactcgatg gtcctcacgg acttttatgt
      481 agagaacatc agtcatcaga taaggataat tcgtacctga gagtgaacgg ttctgccatt
      541 cgttgaccat cgggtctaac tgctcagtca ggctagaaac aaaagatttc gatacagatt
      601 ttccacatag ctcttcaaca atctttgaaa ctttacgtgt cgaaacgccc gaaacataca
      661 tctcaagcat tgaagcgagc agtgcctttt catttcgctg ataacgctca aacaccgtcg
      721 gtgaaaattc accatcacgt gttctaggca cttttaattc gagtgtaccc acacgagtcg
      781 taaagtctcg ctcataatag ccatttcttt gactctgacg actttctgaa cgttcatagt
      841 catcggcttg aatatattct gttcgttgat tttccatcaa ttggttgaaa acagtggtta
      901 aaatattttt agaaacatca tcttttaccg aatgttcaat aatactttga acctcttcgt
      961 tgttcagtgt aaaatgtact tgggtcatgt aacgtcctcc tgggtatgtt tttgtggtta
     1021 aaaacattgt accgtaaaag ggctgttaca tggcctttta tcttttacac aattatatgg
     1081 actttatcct ataatctgat aaattattaa gggcaaactt atgtgaaagg gtgataacta
     1141 tgagcgataa aatacttatt gtggatgatg aacatgaaat tgccgatttg gttgaattat
     1201 acttaaaaaa cgagaattat acggttttca aatactatac cgccaaagaa gcattggaat
     1261 gtatagacaa gtctgagatt gaccttgcca tattggacat catgcttccc ggcacaagcg
     1321 gccttactat ctgtcaaaaa ataagggaca agcacaccta tccgattatc atgctgaccg
     1381 ggaaagatac agaggtagat aaaattacag ggttaacaat cggcgcggat gattatataa
     1441 cgaagccctt tcgcccactg gagttaattg ctcgggtaaa ggcccagttg cgccgataca
     1501 aaaaattcag tggagtaaag gagcagaacg aaaatgttat cgtccactcc ggccttgtca
     1561 ttaatgttaa cacccatgag tgttatctga acgagaagca gttatccctt actcccaccg
     1621 agttttcaat actgcgaatc ctctgtgaaa acaaggggaa tgtggttagc tccgagctgc
     1681 tatttcatga gatatggggc gacgaatatt tcagcaagag caacaacacc atcaccgtgc
     1741 atatccggca tttgcgcgaa aaaatgaacg acaccattga taatccgaaa tatataaaaa
     1801 cggtatgggg ggttggttat aaaattgaaa aataaaaaaa acgactattc caaactagaa
     1861 cgaaaacttt acatgtatat cgttgcaatt gttgtggtag caattgtatt cgtgttgtat
     1921 attcgttcaa tgatccgagg gaaacttggg gattggatct taagtatttt ggaaaacaaa
     1981 tatgacttaa atcacctgga cgcgatgaaa ttatatcaat attccatacg gaacaatata
     2041 gatatcttta tttatgtggc gattgtcatt agtattctta ttctatgtcg cgtcatgctt
     2101 tcaaaattcg caaaatactt tgacgagata aataccggca ttgatgtact tattcagaac
     2161 gaagataaac aaattgagct ttctgcggaa atggatgtta tggaacaaaa gctcaacaca
     2221 ttaaaacgga ctctggaaaa gcgagagcag gatgcaaagc tggccgaaca aagaaaaaat
     2281 gacgttgtta tgtacttggc gcacgatatt aaaacgcccc ttacatccat tatcggttat
     2341 ttgagcctgc ttgacgaggc tccagacatg ccggtagatc aaaaggcaaa gtatgtgcat
     2401 atcacgttgg acaaagcgta tcgactcgaa cagctaatcg acgagttttt tgagattaca
     2461 cggtataacc tacaaacgat aacgctaaca aaaacgcaca tagacctata ctatatgctg
     2521 gtgcagatga ccgatgaatt ttatcctcag ctttccgcac atggaaaaca ggcggttatt
     2581 cacgcccccg aggatctgac cgtgtccggc gaccctgata aactcgcgag agtctttaac
     2641 aacattttga aaaacgccgc tgcatacagt gaggataaca gcatcattga cattaccgcg
     2701 ggcctctccg gggatgtggt gtcaatcgaa ttcaagaaca ctggaagcat cccaaaagat
     2761 aagctagctg ccatatttga aaagttctat aggctggaca atgctcgttc ttccgatacg
     2821 ggtggcgcgg gacttggatt ggcgattgca aaagaaatta ttgttcagca tggagggcag
     2881 atttacgcgg aaagcaatga taactatacg acgtttaggg tagagcttcc agcgatgcca
     2941 gacttggttg ataaaaggag gtcctaagag atgtatataa ttttttagga aaatctcaag
     3001 gttatcttta ctttttctta ggaaattaac aatttaatat taagaaacgg ctcgttctta
     3061 cacggtagac ttaataccgt aagaacgagc cgttttcgtt cttcagagaa agatttgaca
     3121 agattaccat tggcatcccc gttttatttg gtgcctttca cagaaagggt tggtcttaat
     3181 tatgaataac atcggcatta ctgtttatgg atgtgagcag gatgaggcag atgcattcca
     3241 tgctctttcg cctcgctttg gcgttatggc aacgataatt aacgccaacg tgtcggaatc
     3301 caacgccaaa tccgcgcctt tcaatcaatg tatcagtgtg ggacataaat cagagatttc
     3361 cgcctctatt cttcttgcgc tgaagagagc cggtgtgaaa tatatttcta cccgaagcat
     3421 cggctgcaat catatagata caactgctgc taagagaatg ggcatcactg tcgacaatgt
     3481 ggcgtactcg ccggatagcg ttgccgatta tactatgatg ctaattctta tggcagtacg
     3541 caacgtaaaa tcgattgtgc gctctgtgga aaaacatgat ttcaggttgg acagcgaccg
     3601 tggcaaggta ctcagcgaca tgacagttgg tgtggtggga acgggccaga taggcaaagc
     3661 ggttattgag cggctgcgag gatttggatg taaagtgttg gcttatagtc gcagccgaag
     3721 tatagaggta aactatgtac cgtttgatga gttgctgcaa aatagcgata tcgttacgct
     3781 tcatgtgccg ctcaatacgg atacgcacta tattatcagc cacgaacaaa tacagagaat
     3841 gaagcaagga gcatttctta tcaatactgg gcgcggtcca cttgtagata cctatgagtt
     3901 ggttaaagca ttagaaaacg ggaaactggg cggtgccgca ttggatgtat tggaaggaga
     3961 ggaagagttt ttctactctg attgcaccca aaaaccaatt gataatcaat ttttacttaa
     4021 acttcaaaga atgcctaacg tgataatcac accgcatacg gcctattata ccgagcaagc
     4081 gttgcgtgat accgttgaaa aaaccattaa aaactgtttg gattttgaaa ggagacagga
     4141 gcatgaatag aataaaagtt gcaatactgt ttgggggttg ctcagaggag catgacgtat
     4201 cggtaaaatc tgcaatagag atagccgcta acattaataa agaaaaatac gagccgttat
     4261 acattggaat tacgaaatct ggtgtatgga aaatgtgcga aaaaccttgc gcggaatggg
     4321 aaaacgacaa ttgctattca gctgtactct cgccggataa aaaaatgcac ggattacttg
     4381 ttaaaaagaa ccatgaatat gaaatcaacc atgttgatgt agcattttca gctttgcatg
     4441 gcaagtcagg tgaagatgga tccatacaag gtctgtttga attgtccggt atcccttttg
     4501 taggctgcga tattcaaagc tcagcaattt gtatggacaa atcgttgaca tacatcgttg
     4561 cgaaaaatgc tgggatagct actcccgcct tttgggttat taataaagat gataggccgg
     4621 tggcagctac gtttacctat cctgtttttg ttaagccggc gcgttcaggc tcatccttcg
     4681 gtgtgaaaaa agtcaatagc gcggacgaat tggactacgc aattgaatcg gcaagacaat
     4741 atgacagcaa aatcttaatt gagcaggctg tttcgggctg tgaggtcggt tgtgcggtat
     4801 tgggaaacag tgccgcgtta gttgttggcg aggtggacca aatcaggctg cagtacggaa
     4861 tctttcgtat tcatcaggaa gtcgagccgg aaaaaggctc tgaaaacgca gttataaccg
     4921 ttcccgcaga cctttcagca gaggagcgag gacggataca ggaaacggca aaaaaaatat
     4981 ataaagcgct cggctgtaga ggtctagccc gtgtggatat gtttttacaa gataacggcc
     5041 gcattgtact gaacgaagtc aatactctgc ccggtttcac gtcatacagt cgttatcccc
     5101 gtatgatggc cgctgcaggt attgcacttc ccgaactgat tgaccgcttg atcgtattag
     5161 cgttaaaggg gtgataagca tggaaatagg atttactttt ttagatgaaa tagtacacgg
     5221 tgttcgttgg gacgctaaat atgccacttg ggataatttc accggaaaac cggttgacgg
     5281 ttatgaagta aatcgcattg tagggacata cgagttggct gaatcgcttt tgaaggcaaa
     5341 agaactggct gctacccaag ggtacggatt gcttctatgg gacggttacc gtcctaagcg
     5401 tgctgtaaac tgttttatgc aatgggctgc acagccggaa aataacctga caaaggaaag
     5461 ttattatccc aatattgacc gaactgagat gatttcaaaa ggatacgtgg cttcaaaatc
     5521 aagccatagc cgcggcagtg ccattgatct tacgctttat cgattagaca cgggtgagct
     5581 tgtaccaatg gggagccgat ttgattttat ggatgaacgc tctcatcatg cggcaaatgg
     5641 aatatcatgc aatgaagcgc aaaatcgcag acgtttgcgc tccatcatgg aaaacagtgg
     5701 gtttgaagca tatagcctcg aatggtggca ctatgtatta agagacgaac cataccccaa
     5761 tagctatttt gatttccccg ttaaataaac ttt
//
LOCUS       DAJSBL010000099         5790 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.102, whole genome shotgun sequence.
ACCESSION   DAJSBL010000099 DAJSBL010000000
VERSION     DAJSBL010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5790
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.102"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(99..1229)
                     /locus_tag="ONT85_002685"
     CDS             complement(99..1229)
                     /locus_tag="ONT85_002685"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HCR2379616.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     misc_binding    complement(1288..1515)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(1877..3079)
                     /locus_tag="ONT85_002686"
     CDS             complement(1877..3079)
                     /locus_tag="ONT85_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR2379617.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3139..3342)
                     /locus_tag="ONT85_002687"
     CDS             complement(3139..3342)
                     /locus_tag="ONT85_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 family protein"
                     /protein_id="HCR2379618.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3484..4716)
                     /locus_tag="ONT85_002688"
                     /pseudo
     CDS             complement(3484..4716)
                     /locus_tag="ONT85_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173388.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
     gene            4879..5691
                     /locus_tag="ONT85_002689"
     CDS             4879..5691
                     /locus_tag="ONT85_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR2379619.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
BASE COUNT         1717 a         1189 c          921 g         1963 t
ORIGIN      
        1 aaataaataa tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg
       61 actttttggg acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg
      121 acttgctgca ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct
      181 tctgttgaag caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt
      241 tgtgcttctt ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa
      301 gtaactaatc ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa
      361 tctcctgctc ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac
      421 tcttctgcaa tgggttcata tccaccggaa aacagccaag aagaagcgtg attcccgcgg
      481 cagatatgtg ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc
      541 tctgtacaaa tatgggccat ttctttcgga tcaattcctt tttgaagaag tgcttctctt
      601 ttagtttgat cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct
      661 ttttcataaa aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca
      721 gatagatcag gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt
      781 cgtaaaatca tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc
      841 actacttctt tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat
      901 ttatctacta cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat
      961 ccagtttctg gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa
     1021 aatccacgcc gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc
     1081 actaattttt ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct
     1141 tcttgaactt tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga
     1201 tctactgtaa atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa
     1261 aaaaatcctt ttactagcaa atactagtaa aaggacgatt atactcgtgg taccaccttt
     1321 tttcagaaaa aacattcttt ctctcagcct gacaataaaa tcagtggtct tggtaacgaa
     1381 agactgttcg tttttgccta cctttcgaca aaaaatcctc taaggccatt ttcgacagtc
     1441 aacttctagc tttttacacc aaccaaagcc tctcttgaac cattttctgt ctactttcct
     1501 tctcatcgga tactattaga ataaaattag aatagcatgt ttgaaagaaa agtcaatagg
     1561 gaataagaac aaacaaaaac taatttgtta ttcttagtaa tttgtttgtg gaatacaata
     1621 aagcttttct aatatgataa ttgttcagtc gtcctttcat cttagatcac ttccttaaaa
     1681 tgatcatacc cgtttcaacc acataaaaaa ggacttaatt ttgaaaaatt tcccatcaaa
     1741 actaagtcct ttttattata gttatgcaaa tttgcttatt ttctcgctat ttccgataca
     1801 tcttgaacag gagcaattcg actgaaaaca ctggtgcaaa agcatttatt tgtcttatgg
     1861 tagaaccgtg gaaaactcag actggtaata aattgtcatt ttttgaaaga atagcgccct
     1921 cttgaacatt atcaatcgca cttaccactt gttgatctga ctttgcatat aagtgaccgt
     1981 aagttcctaa agtgatttga atattcgcat ggcccaatcg ttcttgaata gatagcggtt
     2041 gaatattctg ctcaattaga aatgcaacat gagaatgtcg tagcgcgtga agcttaatcg
     2101 gtttgacacc cgctttttta gcagcttctt ttaaccagtt cgcaaaagaa taaggtggcg
     2161 taaatgttcc gtccaactga gcaacatatt caaaatttcc aatacgtttt tgcaagtcat
     2221 accactgtct gagttttaag gaaagcttct tccccaaccc aatcgttcga taagcacatt
     2281 tagttttggg agtcgaaaag taccaatcat cttttgtatt ataaatcaat gtcttctcaa
     2341 tcgttatcgt gtttttctca aaatctacat tcgaccaaga caaagcttca agttctccaa
     2401 tccgaatgcc agtataaaag agcatttcat acacaatctt ccgatggtgg gtttgaatct
     2461 tatcatcctt caattcattc atcacttttt gaaactcact gtgttcccaa aactcaactt
     2521 tcttattctc agaaggaagc gaagaaacat cttcaacggg atttttagca atcaccccct
     2581 ctttaacagc ccgatccaat acaattttta ggtgccctcg aatccgattg atataagatt
     2641 ttgataacgt ttcgccatta acttttttcc gtgaattacg tgactctgtc tggcttagtt
     2701 caaataacca atcctcaata tcgtgacttg taatatctcg aacacgaaaa tgatagaaaa
     2761 actgcatacg cttttcgaaa atgaattgcg cattctcata tgtttgactt ttgacctgtc
     2821 tctcgtacca ttttacaaac ctatccttgt aaaactcttc gaaagtcatt tgattggtag
     2881 aacacagttg attaggaatt gtcttgttaa aatcatgtgt ttccatgtac ttgtcataag
     2941 caattttggc atctttttgt gaagcataac ctcgtttagt cttctgaacc tttcttccat
     3001 tttcatctat acctagatac gctcggaaga accatttttt tgtcttttta tcttggtaaa
     3061 tatttttaat ttttggcatc gttccattcc tccctgtact tgagaaaagc tactttaata
     3121 gtaccgcgct tgtactgttt attccgcaac ttgaatgtga aaaagatgtt caattactcg
     3181 ggatggcacc acaccaactt ggcgattatt ataaaaatca ataccttcaa tccttgcaag
     3241 atatgccttg ctctctttaa tcattcgtgc agcttgacta ggtttaaagc ccatctctat
     3301 caaaattgca cgatttacca tgaaatcaca ttttgtttcc attctctcac ctcctgattc
     3361 tttactcctt ttaaaataat cctctttata atctattgtt tctcattcga tggttgtgtt
     3421 atactcgtct tgtcgaggcg gagtacgttg cacaacttgt tgtgtaagca gtactttgct
     3481 tttttagtag cttgttttct catttgatat actttgtaat tttcgttcat aatactcaac
     3541 ctcctcctga taatactccg ggtctgcctt catattttgg agaagtttga tctgtcttgg
     3601 attcatttct ccttgttcaa taatcaaagg caacaaggaa gaatgaaacg tttgatcgac
     3661 ctgtgcaata aaaactaatg atggtgaaac accatgaatt aaccaactca tagttttctc
     3721 aaaactttga ggaattgtat ctaaagaaat ggtgattgcg ccataattgc taataaaggc
     3781 tgcccatttg gcatcaactt ttgcacctag atcatttgga tcacggtcat aaaaacaaat
     3841 tgctccatta agtaactcaa agaacagttt ggttaaatca tgggtttttg aaataatctt
     3901 tgccaagctt tgcgctttct cttttcgata ccgtagttca aagcgattct ttacttttgc
     3961 ctcttctaaa ggaattcctc gtcgcttccg ttgttcatgg tccttctgat aaaagcaaaa
     4021 acgacactga ctcttctttg agcctaaata caaggtctca ccatttgatt cattgtttat
     4081 gatatccgta ccgccatgag attcactcac ttgaaaagtg gtccacacat ggcctagtat
     4141 taccttatgc ttaagttctg gaatgctgag actccccaca aaatcatcta aagtaaaatc
     4201 aatccgtgta aagttcccct gataatctaa taccttacga aaaaactgtt gccaatttgt
     4261 ttttcgtgtt tttagccatc ttgctaagtg tttacagcct tgacctgaaa actccatcac
     4321 agttccttta accgcatcgt caatggaata ccgaatcgtg attacttgat tccacacgta
     4381 ttggccaata tagccctttg gcgcataatc cagttcctca aaacgttcaa tgggcatttc
     4441 aataatctct tcaattagct ttaaatgttg attcgagaaa aagtgaatgg ttagataatc
     4501 aatcaaaaca cggttaactg gaacgacctc ttcttgcaaa ctatccaaca caagattcaa
     4561 tttttcttca cttaattgtc tcgttccttt ttctactttc gataataact atctcgaaat
     4621 ccccactttt tctgctaaat atcgttgtgt ccaccccctt tcttctcgag aaactcgaat
     4681 ggcttcaccg agatcatgtt gattaacttg gttcatcgac aaacctcctt ctgctattta
     4741 cttgtcaaag aacctattga acttatttta agtacatttt tattgtacgc ctgtatttat
     4801 tttaagtcaa tatgttttga ctttttttaa gtacacacgt atactaaatt taagaactta
     4861 tcctgaggag gagttttcat ggagatagac aagcaagcag tcggcaatcg aattcggcag
     4921 attcgccaag aattaaagtt gagtatggag aaatttggca aactaattgg tgatttaccg
     4981 cgaagtacag tcaataactg ggaacgtggg atcaaccttc caaaaaccga aacccttcac
     5041 caaatcgcag agataggtca tgtcaccaat gaatatcttt tgtatggtga tcaagagaat
     5101 caatacattt tagaaatgct ccaaaaaaag gcaggtaagc ttgatcctaa gattgaaggt
     5161 ctcatagtag acgaaatcaa acacgctgac cttgttagtg aaaaagaaat ggatcgaatg
     5221 attgaatttt ttattacaaa tctcattcca ccaactgaac aagaccagtt tactttccaa
     5281 tgtatcgatg aaaaaaagca gctttatctt ggttcaacta attttggtaa ggaagcccag
     5341 ctctatttgc aacatgataa tcaaaatcat attctgcaca tgatgccctt taccttctcc
     5401 aactttagtg tggatcgcat actcgtctat ttagcaaatc aaaaatccta ttcctatttt
     5461 gggaaacatt tacccaagga actaggtgag aaagccatcc ttctatactc aattaatcaa
     5521 ttgaccagcg atgtgcgaat tgccccgttg gtctattcag aagaaaccgc ttcttatcag
     5581 tacacagagg acaatcaata tctactagaa cagcaatatc tttatctacc attcgtgatg
     5641 gaagtcgaga aagaacgcct gttaaatgca gcgtatccat catcaaaatg aggtgacaaa
     5701 attgataagc acgtgacaaa gaaaatactg ctaggacact ccccaatggg tagttgtcac
     5761 agtttcctgt tctttgttag ccccctgtta
//
LOCUS       DAJSBL010000100         5349 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.103, whole genome shotgun sequence.
ACCESSION   DAJSBL010000100 DAJSBL010000000
VERSION     DAJSBL010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5349
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.103"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            25..696
                     /locus_tag="ONT85_002690"
     CDS             25..696
                     /locus_tag="ONT85_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR2379620.1"
                     /translation="MNFLVVLKDTLIERSTFLYVQLQENKTLLHFSPEFHLEGLTLGE
                     IYQAEGLSAVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTA
                     AELQKQMRYQIDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSI
                     QFPQLITMIHRFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVA
                     MEKAN"
     gene            755..1195
                     /locus_tag="ONT85_002691"
     CDS             755..1195
                     /locus_tag="ONT85_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2379621.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            1207..1875
                     /locus_tag="ONT85_002692"
     CDS             1207..1875
                     /locus_tag="ONT85_002692"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HCR2379622.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            1939..2562
                     /locus_tag="ONT85_002693"
     CDS             1939..2562
                     /locus_tag="ONT85_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HCR2379623.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            2618..3004
                     /locus_tag="ONT85_002694"
     CDS             2618..3004
                     /locus_tag="ONT85_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HCR2379624.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            3217..4608
                     /locus_tag="ONT85_002695"
     CDS             3217..4608
                     /locus_tag="ONT85_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HCR2379625.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
BASE COUNT         1775 a          879 c         1124 g         1571 t
ORIGIN      
        1 aatgatgaaa aaagggggag aaaaatgaat tttttagttg ttttaaaaga tactctgatt
       61 gagcgatcta catttctata tgttcaattg caggaaaata aaacattgct gcatttttca
      121 ccagagttcc atctagaggg gctaacatta ggagaaatct atcaagcaga agggctgtcg
      181 gctgttttgc acttttttaa ggtagaattg gaattaccag taaaagatta tatcgagctt
      241 tctcttgaat cttgggatcg agcagtggtt gaactgtttc cagaaggttt aatgatcgat
      301 acaaacgatg ggaagatcca tctgactgca gcagaactgc aaaaacaaat gcgttatcaa
      361 atagatgacg aaaattcttt ttctattttt agacgtcagc aaaagattct aaaaagcttt
      421 ttaaaagtga tcagtaaaaa acggaatctg atcaaaacga cacagctgtt gaaaaaatat
      481 ccaggtctgc ttcacacgtc tattcaattt cctcaattga ttacgatgat ccatcgtttt
      541 atacgtatgc aacagctacc atctaagaag ctgtttcttc cattgacgga cacatttcga
      601 gtagtcaaca gagaagataa aaagaaagtg attgtgattg actttaccag aaatcggaat
      661 gtccttcacc aagttgcaat ggaaaaggcc aattaaggct ttttcttttt ggattttttt
      721 gtcataataa atattagatg atggaggaga taatatgatg caaacatggt tagaaaggct
      781 aatacgattt cagcagaact atcacaaact tatgcaggga agatatgcga aatttgatca
      841 gttgaatcgc ggtctattga tcgctgcgct tgtctttagt ttgctaagtc gttggttgcc
      901 ctatagaatc ggacaattgt tgttcatcgt ctttttcgga tggatgatat ttcgtttcct
      961 atcaaaaaaa atctacccca gactaaatga gaatcaacgt tatttgaaat atcttgatag
     1021 agtcaaagaa aaatatcatc agataaaaaa caaaaagcaa gtcaaaatgg atcgaaaaag
     1081 ttatactttt tttgagtgcc cgaattgtca tcaaaagcag cgtgcaccta aaggaaaagg
     1141 acgaatacgt gtgacatgca aaacatgtgg tataaaattc gaaacgaatg tttaagggag
     1201 aaaagaatga aattagtatt attacgtcat ggcgaaagcg aagcaaattt tgaaaattac
     1261 tggacaggat ggctagatgt cgccttaaca gaaaaaggaa aagaacaagc agcagttgcc
     1321 ggtcaaaaaa tgcttgaagc cggacttcaa ttcgatgccg tttatacctc tgttttgaaa
     1381 cgtgcgattc ttaccggaca agtcgcacta gaagaaatgg gtcagttatg gcttcctata
     1441 ataaaaacat ggcgtctaaa tgaacggcat tatggggatc ttgtgggtaa aaacaaagat
     1501 aagatgaaag aaatatacgg aaaagatcaa gtaaaaacat ggcgcagagg atatacggaa
     1561 gttccgccgt taaccgcaga aaatagattt gaccgcagat atgatcactt ggatccgcat
     1621 ttgatcccat acggagaaag tctggaaatg acagtgaatc ggatcattcc cttatggcaa
     1681 gatcatctag caccaaaact aaaagatcgg caagatttac tagttgtggg tcatggtaat
     1741 agcatccgag cactgacaaa atatttagaa gatgttcctg aagatcaaat ggatacgatc
     1801 gatatcccaa atgcacaacc aatccagtat acattagcag atgatttgac aattctggac
     1861 aaaaaaatac tttaaagaac acctttatct aaaagtgaga gatagaaaaa agaagcaaag
     1921 gaatggaaca aaaggaacat gaaaaattat atatgggact ttgatggtac attgtttgat
     1981 acgtatcccg caatggtaga tggtgcatgg caagcactaa aagattttgg tatcagtatg
     2041 gacaaaaaag aaatctactt caaaatgaaa aagtattcta ctagttattt gatcaatgaa
     2101 tcaaacttga atgccagaga gtttaatgaa ttatttcacc gatatgaaaa agaaagtacg
     2161 gaagtttccc gtccatttcc agaaacgaaa caagtactgg aaatgctaaa agacaacggc
     2221 ggtcgtcatt tcatcctgac ccaccgtttg acagaatcaa catggggcct gttaaaagaa
     2281 catcgattgg cacatctgat tgaggaagta gtagggatcg atcaggactt tcctcgtaaa
     2341 cctgatccag catctttaaa ttatttgatc gatacttttc atttagaaag aacagacacg
     2401 atgatgatcg gcgatcgtcg tttagatatc gaagcaggga aaaatgcagg agtcgctact
     2461 tgtctttacg acatcgatca ttttctgggt gagatcccgg cagattatgt ggtaggaaat
     2521 ctgaatgaaa tccttacctt gcagagcatc attaaaaagt agtatcccct ttcaaaggag
     2581 atatgttata attttcataa aggagtgata aaattatatg gaaatcagaa gacaaaaaga
     2641 agttgtagaa gcatttcatt atgatatgag attgccgaac caagaagtag aaacagattt
     2701 gaaagtaggc ttctcgcctt taaaagaaac gatcgaaaac tatccgaaag aacatagcat
     2761 cgtaggagca cgcttagagt tccgtttagt gtttgataca tatgttttat ctggaagtgt
     2821 gagtcagatc aatcatatcc tcaatcggaa aattgaaggt caaaatgatg tcacacaaga
     2881 agaagtggac gagctagtag caccgttatt cgatatcgta cgccgaatgt cttatgaggt
     2941 gacagagatc gccttagatc agcctggggt aaaattgaat tttcaatcag aaaaacaaga
     3001 atagacagta aaaacatacc tgcgatagat taaagctatc acaggtatgt ttttttatta
     3061 ctaattgtaa gcaaaaagaa tgtcatttcg ataaaaatcg tctaaaatat aaaagtcact
     3121 gaaaaaaatt ttttcaggag gatggttgaa gaatcattct cgggaacctt tacttccttc
     3181 gatggacttg tcattaataa tatgaggagg agaaaaatga caacactcat cacagtttta
     3241 gtggttctag tttttgcggc attattatat gtgttctatc gtatgcaaaa gcgccataca
     3301 aaattttcaa cacgcgtctt tacagcatta ggagttggta tcgtctttgg cggtctgctt
     3361 caattgatct ttggtgtaga cgacaaagtc gttactcaat caatggaatg gatcgggatt
     3421 gtaggtcaag gatatgtatc attcttgcaa atgctagtta tgcctttagt gtttatttcg
     3481 atcattggtg cattcacaaa aatgaaagtt tctgaaaagc tagggaaaat tagtgcgact
     3541 gtattgactt ctttacttgg aacaacagct attgcagcat tgatcggaat tggttcaaca
     3601 atgctgttcg gactccaagg agctaaattc acacaaggag ctgcagaaac agcacgaatc
     3661 tctgaactgg caacacgtca ggaaacagtt gaaaacttat cgattccaca gcagatttta
     3721 tcctttatcc ctaaaaacgt ttttgctgat ttagcaggta gtcgtgcaac aagtacgatt
     3781 ggagtcgtta ttttcgcggc atttgtcgga atcgcttatc taggcgtgaa aagaaaagcg
     3841 ccaaaagaag cagcattctt ttcagattta gtcgacagtc tgtataaaat cattatgcgg
     3901 atcgtgacgc ttgttttgcg tttgacacct tatggagtat ttgctttgat gacaaacgtt
     3961 ttggcaacaa gtgattttgc agctatcgtc aatttaggta aatttgtcgt tgcttcttac
     4021 gtagcattgc ttgctgtttt gattgtccac tcactgattt tgattggtgc aaaagtcaat
     4081 ccaatcaact atttcaaaaa agcctttccg gtattgagct ttgcgtttac ttcacgttca
     4141 agtgctggag ctttgccatt gaatatcgag actcagacaa aagctttagg agtagatgat
     4201 gcaacagcta atttctcagc aagttttggt ttgtctattg gacaaaacgg ctgtgctggc
     4261 gtttatccag ctatgttggc aacgatcgtc gcaccagcag ctggagtgaa tgtctttagt
     4321 ttggaattta ttttgatgct tgtggcaatt gtaacaatca gttcttttgg tgtagctgga
     4381 gtcggtgggg gcgcaacatt tgcttcactg atcgttttag gggcaatgaa cttgccagta
     4441 gcaatcgttg ggttagttat ctctgttgaa ccattaatcg atatggcacg tacagcagta
     4501 aacgtcagcg gtagcatggt agctggtatc gtgacaagtg cacgcatcaa agatttagat
     4561 cgcgatgtca tcaatgatga ttctaaagtg atcgatgtga atgcatagtc aaaaagaggt
     4621 tgtgatagaa gtggtcagct ttaagaaata agaagaagtt tcctaaaaat agtttctcaa
     4681 atttttgtga aatttcggct tatttccgag aagctacttc tgaaacaccg tgaataagag
     4741 gttgtgaaaa agctgccctg catcaagtaa taagaacagc caaacaaaaa tagttcctca
     4801 tattttacgt ttggttgagc ttattaccgc agatgcaagc ttttgaacac cgtttaagaa
     4861 agaggtcgtg agcctgccgt ttcactctga gtcacaaaga gcaatttcaa aaaatagctt
     4921 ctcatgtttg ttgaatattg tgtcttgtga ccgaggagtt gtcaggagaa caccgtttac
     4981 cagaggttgt gacttgtttt tagtcacgac ctcttttttt atttattcca tataaataca
     5041 cattatttat tccgaaaagt gaaagcgcaa ataacaaaaa caaccaactt atttattatt
     5101 aaagaaaagc gtatttaagt aggacgattc tgttgagagc tttcgtagat tttcccattt
     5161 tcctatggta aactgacaaa gttgagaatg ttagaacaga tcgtttagtc taatggtgat
     5221 aagtcaagat gaaatttaag attttcttta ttgagtaata tgttttgaag cgcacgaaac
     5281 tagacccgat caaaatcaaa tttttttcgg gttgattcaa ggatttgcgt caataacgga
     5341 ttgttattt
//
LOCUS       DAJSBL010000101         5340 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.104, whole genome shotgun sequence.
ACCESSION   DAJSBL010000101 DAJSBL010000000
VERSION     DAJSBL010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5340
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.104"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(61..279)
                     /locus_tag="ONT85_002696"
                     /pseudo
     CDS             complement(61..279)
                     /locus_tag="ONT85_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391850.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(545..739)
                     /locus_tag="ONT85_002697"
     CDS             complement(545..739)
                     /locus_tag="ONT85_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2379626.1"
                     /translation="MMGFFLRFYIIELTGRLTVFFGNFAIKPFAEVTAHYICKWLDCK
                     NKIKKVFHYISSSPCQIRPG"
     gene            complement(839..925)
                     /locus_tag="ONT85_002698"
                     /pseudo
     CDS             complement(839..925)
                     /locus_tag="ONT85_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
     gene            2091..3131
                     /locus_tag="ONT85_002699"
     CDS             2091..3131
                     /locus_tag="ONT85_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HCR2379627.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESGISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYE
                     SSPSTITKDWLE"
     gene            3955..4287
                     /locus_tag="ONT85_002700"
     CDS             3955..4287
                     /locus_tag="ONT85_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HCR2379628.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIG"
     gene            4906..5247
                     /locus_tag="ONT85_002701"
     CDS             4906..5247
                     /locus_tag="ONT85_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HCR2379629.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSF
                     EIIEFVADKEE"
BASE COUNT         1943 a          728 c          900 g         1769 t
ORIGIN      
        1 aggtattttt attatatata gatttaacat atgtcggtcg gttgattgat atagctaaag
       61 ttataactca accgaagccg attcatctac acgaataaag ttgcgagtgt tctcggctaa
      121 ttcataaaaa ttgttatgtg ttccatatag caatactgga ataactttat tttcttgaat
      181 ctcataagca actctataca cggtgaccgc atacttaaat ccattagacc aattacctga
      241 taaacctcca gttttttgct cttacttaaa tggatcaatg gcaatttcat cacaaaccta
      301 tagaggatga gtgacttctg cctaattacg ttaaaaaata ttgaaagtaa ctctatgtag
      361 caaaaaatat ctagaatttc atttcctcaa aactgatcga tattgtatct aaaaatatat
      421 actacctaaa ataggtagta tatattaaaa tatgtgcttt tgttagaaaa atcaatacat
      481 aaattatcat atctgtatat gtcatttagg tatttcagtt atgttataat agttttgctg
      541 tctgctaccc cggcctaatc tggcaagggg aggaggagat ataatgaaat acctttttga
      601 ttttgttttt gcaatcaagc cacttgcaga tgtagtgtgc agttacctct gcaaatggct
      661 tgattgcaaa attgccaaaa aagacagtaa gcctacccgt taattctata atatagaaac
      721 gtaaaaaaaa ccccatcagc ctgcacgctg atggggtttg gtgatataat gaaatacctc
      781 tttgttactc ataattgtac cataattagt cacgaatgca accaatagaa aaaactactt
      841 agaacggtag atcatcgcta ataacaatgc attgtccacc aaatggattt tgtgaagcat
      901 taaatacatt gttattaatt gtcgctgctt ttaactaggc ctctcccctt ctattataaa
      961 agaagatata tatcatttct taattaccta ctaccttaaa ccaaaattta agtactacct
     1021 aatttaagta ttacttaaac aatctaaaaa atagttatcc caattgttat gcttttataa
     1081 tacgattatt tgtaacaaaa tgaatattat ataattaaac actaccatat ttaggtagtg
     1141 tttaattatt agcaaaacaa aaaatattta ttctattctc ttaacttcaa ctaagttatg
     1201 ctgcacacct ttatataaat catatgcaac tcattagcta gacaaactac aagcatgtag
     1261 ttttatctgt taattatgta ctacctaaat aaagtagttt ataattagca agtatatata
     1321 tttaaaaatt taagattaat ttttttatga cttgtacaaa tttatctgat attctcttat
     1381 taagaactac ctaatttggg tagttcttat tttttacatt ttaagtagta tttaattgca
     1441 atttggtagt atttttttat tatcttattc ttagaacggt agtacttaat ataaatatag
     1501 gtagtatcta atttaagtat taatggtagt atctaaatta acaggtagta tgtaactact
     1561 ttaaagtaat ttttttcaga ttaacacagt agtatcttat taaataagtt aattttaatg
     1621 gtagtacata attattatat atatctgaca ttggtagtac ttattgtatt ttttagtagt
     1681 atataatcta ttgttttcta gcctaaagcc ttgtcttata agaataatat aatatatact
     1741 actgaaataa ggtagtatat attactagta atggtagtat gttttatatg cataaggtag
     1801 ttcttaattt attttttgtt ttgacactgg tagtacttaa ttatctttaa ttttaaaact
     1861 aaattttatt catacgtttt tctggtagta tttaattata aataatattt atttaaagta
     1921 gttcttaatg actttaaagg tagtacataa ttcattttat ttctgatttt aggtagtatt
     1981 taattaaaaa acagttttaa tgcgtttgca ttttttattt atctttgcta ttttcgaaaa
     2041 gagtaggtaa ttttacataa ataaaacaac gtgaaagtag aggatctaca atgactgatt
     2101 ttaaattttt taaggcggat agagtttaca acgaattatt ttatcaattt ccaaaagtct
     2161 ttattgtttc tgacgaatac aaaaaaatga aagattcaac taagattgcc tatatgcttt
     2221 taaaagcaag attagagatc gcaatcagca aacggcaaat cgatgaagaa ggtaatgttt
     2281 attttactta tacgacaaat gaactatgta gagtattaaa ctgccaaaaa caaaaagcga
     2341 tagcaatcaa aaaagagttg gaatcctttg gtttattatt acaaaagcag atgggattta
     2401 acaaacagtt agggaaaaat aatcctaata gactatatct agcagaatta aaagtctcag
     2461 aaaatgatat ctacttactc gaaaaatttg atagagagaa tagggaaaac gttgataaat
     2521 cagagggtat gaaaatcata cccaccctcg acgaaaaatc agacgctgaa tcccttgggg
     2581 ctcaagaggg tatgaaaatc ataccgtgcc aaaacgttga taaatcagag ggtatgaaaa
     2641 tcataccaga acttaataat aatatattag acactaatag acacaatata gacactgaaa
     2701 aagaccgcct acaagatcaa ttgttgttag acaattttga gacaattatg acaaacgaca
     2761 gcattgctac gtttgtccct gaacgagtat taaatttgat aaaaacattt tcttcaagtt
     2821 acagtgaagc tcaaaaaacc gtccagacta ttcataatgc aaagaaaaaa gctgaaatag
     2881 aaagtggtat ttcgatagtt tttgaagaac tcgatagtta ttatgtcaat gcagaacaag
     2941 aattatacac gacactgtta aaagcctatc aaaaattaaa aaccgaaaaa gtcgaaaata
     3001 tccagaacct gatttttgtc tatgtaaaaa attggtttat cgaaaaacca atagctgcta
     3061 aagtatcaag tgaaaaacgt ttgaattatg aaagctcccc aagcactatt acgaaagact
     3121 ggttagagtg aaactttact gacaaagtga ttgttgcttc atggagtaac attaaaatta
     3181 aaaatttttt attattctaa ttatattacg aatattacaa aataatagct attttaatag
     3241 aatttataag ttaaaatatt atgataaaaa tcacaaacgt agccttttac gtcataagtg
     3301 ttaaaagaaa taattaattt caaatataga aaagggaacg tatgtttgca tactcctttt
     3361 ttttgagtta taataatacg cgaagagaga taatttcatt atttaaacaa gctaaaaatc
     3421 ttgatattac ttcttttcag gcaatacgaa tattattcgc ttacattttt tgtataaatt
     3481 taattgtctg ctatgcttgt gatgaattga ttttcgttct gcacttttgg atgataaatt
     3541 tggacaattg aaaatacaaa aaaaagaaag gcggtatgaa ctcggaaggc tggtaacctt
     3601 cgtcagcgtt catacacggc attaagattt ggcccttaat gcgttaacct ttcagtgtta
     3661 cggtattacc cgcaatggat ttagtataac agatattcaa aagtgatacc actttttttg
     3721 gctgtgttaa ggttaaaaat acgtgatatt acgatgaatc tatgcctaag cacaatgtta
     3781 taaaaatgtc cgtgtggctc tttgccgtat gacgaacgct gaaattagag ggaacgcatg
     3841 tttggatgcg ttccctcttt tttgtttgtc atgaacgaaa atcaattctc tatttttgaa
     3901 aaataacaag aaaaacttgt taaaaatatt taaagaaatt gggtggttta ttttatgtca
     3961 tatacaggaa ttttgagtct caaagatatt tgtcactacg gtaaacggtg taccgctaca
     4021 gagaaaatca cgaagaaact atcaactggc caaagtaaaa ccgttgtgca gtgtaagaaa
     4081 tatataatcc agaaagacaa agtctcagag gaaatgattt attacattgg gaaacaaaag
     4141 caaataattt taaaagatcc tatcccctta aaagagctat atccaacaat caaacatgtt
     4201 tatgaccaaa acggtgtact gattggtcga agaaaaaatg gagtcttgcg ttgtaccgca
     4261 aaagggatgg ggcgtttgat cggctgaatt gatcattctc ataacttgta ttttatcgtt
     4321 tttcgcttgc ttgaaaatat tgtcgagact tctaaaggaa cttagagtca ctagaaaaat
     4381 aaggagtaga cttgatctat caacaattcc cttcggggag tgacggggta ggttttaacg
     4441 attggtaacg taaaaaattg gtcaatcgag taaatgcgct tgtttggata gtggtgaaat
     4501 gaatttgtgt aaataataag cgcagagtaa acaagaggga atttagaagt tagacaagtt
     4561 agaatagcgt ctagattaat catgagcagc tactaaggga tctagacttt gacgcaaact
     4621 catggaataa ggagtaggtc ttctcgtaat ggggaggacg cacaagaaat tgtgtcgtgt
     4681 gctgcagtac atgtagatac ttgcgaggac gttgattatt ggatcaatcg agcatttacc
     4741 gctgtctact agggtttctc gtttcggacg ccataaacga gtcgaaattt tttatgtatt
     4801 tttaagtaaa cgaaaaacga taaaatatga acaagtcctg aataaagtga aaaaattgat
     4861 ataaaacata gagaaaatga aatttacgta atggaggaat gacaaatgaa aaattatgat
     4921 ccgaatatac gattgggcac acatactatt aaggtttctt tccaacgttg ggattacaaa
     4981 ggatttgtga cgtttagaag aggcggaaat tgtaaaggat tagatgtttt agcattagac
     5041 gaggatgatt tatacgatca aacattgact gataatccaa ttggattcgg cttgctacct
     5101 gaagatgatg aaggtaatga atggtttaaa atgacgctta tgaatgataa tggagacgaa
     5161 ttatctgttg aagatacttg gtcatatttg agtgattata ttgtcagctt tgaaatcatt
     5221 gaatttgtag cggataaaga agaataaaaa ctggaggaat aaattttaca gataaaggct
     5281 tagaaggagt cacgatgatt aataaaaagg tagtattacc ttaggcattg agtaaggaat
//
LOCUS       DAJSBL010000102         5250 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.105, whole genome shotgun sequence.
ACCESSION   DAJSBL010000102 DAJSBL010000000
VERSION     DAJSBL010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5250
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.105"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..1666)
                     /locus_tag="ONT85_002702"
     CDS             complement(<1..1666)
                     /locus_tag="ONT85_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HCR2379630.1"
                     /translation="MENSTEFFKNIQTEFNHALTPMKPPKHKLVGRDDELHELSLILN
                     RPETPQAILLGEAGTGKTALASEWAERQRQDGKKVQVYKLSIGQLRVGGDDVLQTRLE
                     TLIPRLKEYERILKAEDKDNEFVLFIDEFHKVVSSFGSGNKIGGDSLKEPLANESLQM
                     ITATTRKEYENYIKRDDALGRRLQPLELNELKSDVVVDILRQWLNTSSVKDDPQASFR
                     LSQRIPDELLQEIVQANKLYREDLSEPAKSIAVMEDLIAKDRVDGNAYNRESLLQIFG
                     KYNIQLDSDFDMEKLQEELKSIKGQPLAVYTITMLFKRMKLGFDRFKGRKQPKATALF
                     TGSTGVGKTAMAKAVARGLYGDENKVLLVNMPDYKTKDDAMRFRRLIGTHVRHDPSAI
                     IVCDEYEKAGVETLDAFLSILDEGRISFTERGWDDREVHDTVSLSNTILFATTNAGAE
                     MFDSLNRYSSLSRVTNKENSNEYSTELKREYRKVASGIKQALIGEGLKPEVINRFGRI
                     VPFWALSEQTLLEIAYNEMLKSAELVRKHLNIDLRLPPRKERVYGQN"
     gene            complement(1708..2766)
                     /locus_tag="ONT85_002703"
     CDS             complement(1708..2766)
                     /locus_tag="ONT85_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliS"
                     /protein_id="HCR2379631.1"
                     /translation="MEKNVNSTSTKDQYSIIGGLLGDKDKRTRHGLRIMLATFLGVLF
                     LLTGLGSYIYQLGNYNYYLVNNTTPIGSSLEFARSGAEITISDVWTDQNRDVTVVKLG
                     YSQNARKLLSTQGKNYNLYMATKEKDRPKKLEMSYGMLSTDGDAFLFLKGKLEERAYQ
                     VFIANQVSLISGDDTADDDTKVNVNEADSVTKALSQYSMNDVNNEGVISFGKKAKPVN
                     TPDNINFRINPYSDSTNVYQGSFLTKDGDIDYSKVVAVTSKNNVVNKLEDQLNASNTN
                     IKKIEATKAEYLERLQQNPDDKDSQESIRQLDSRMETEIKAINDTQKLIDQYKNADFN
                     KDSFGDMKTTFKYLTLTD"
     gene            complement(2885..3340)
                     /locus_tag="ONT85_002704"
     CDS             complement(2885..3340)
                     /locus_tag="ONT85_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2379632.1"
                     /translation="MNAINWHEFVNYMESLLDDLKSFSIADAVKHGLPVSTTMKLRGL
                     KIPDEIIKLRTKTIIETAKVVDNMNHELKGKKTTYEKDFERFLKSVIGKYLGKKSFSE
                     LRDEEIVPTPMVSKIRKNKAEGNYYLGTIAKIIDYLENKEQKKGTKKGR"
     gene            complement(3924..4727)
                     /locus_tag="ONT85_002705"
     CDS             complement(3924..4727)
                     /locus_tag="ONT85_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HCR2379633.1"
                     /translation="MSQEVTEKIIDSDVDLVSEQTNAIVRYNNGLNTTALRGFKPVEM
                     DVFWAILSKMKRQGTAEVTFDFNLFRYLVKYDRREKDSFYSVLKALSDKLGTLTYRFE
                     DSDVYEQLWLFQKFRIDKQKETVTIKANEEFEFILNSIGKNFTRFELDNFTKLRSSYT
                     KELYRHLMQYRNKEWRNGYWVVKVEDFRRALSIPSSYRMTNIDKRILEQAKEEFLAPD
                     ENGIQVFETFDYEKIYAKKGRRVDRLRFTFSEPESNLPTISMHNWLEED"
BASE COUNT         1551 a         1026 c          727 g         1946 t
ORIGIN      
        1 aattttgacc atatactctt tctttcctag gtggaagacg taaatctata tttaaatgtt
       61 ttcgtactaa ttcagcactc ttcaacattt cattataagc aatttccaga agagtttgtt
      121 cggataaagc ccaaaatgga actattcgtc caaatcggtt aataacttca ggctttagac
      181 cttcaccaat caaggcttgt ttgatccctg atgcgacttt tcggtattct ctttttagtt
      241 cagtgctgta ttcattagaa ttttccttgt ttgtaacacg agatagtgag ctatatcggt
      301 ttaacgaatc gaacatctcc gcacctgcat ttgttgtcgc aaataaaatt gtatttgaca
      361 atgaaacggt gtcgtgaact tctcgatcat cccatcctct ttcagtaaaa cttatcctac
      421 cttcatctaa aattgataaa aaggcatcga gtgtttctac accagctttc tcatattcat
      481 cacacacaat aatcgcagaa ggatcatgcc ttacatgagt acctataagt cgtctaaaac
      541 gcattgcatc atctttggtt ttgtagtccg gcatattaac taagagaact ttattttcat
      601 cgccataaag tcctcttgcc acggctttag ccattgcagt tttaccgacc cctgttgatc
      661 ctgtaaacag agcagttgct tttggttgct ttcgaccttt aaaacgatca aatccaagct
      721 tcatacgttt aaacaacatt gttatagtat aaactgctaa cggttgtcct tttatggact
      781 ttaattcctc ttgaagtttt tccatatcaa aatcagaatc tagctgaata ttatacttac
      841 caaatatttg caatagactt tctcgattat aagcatttcc gtctactcga tcttttgcaa
      901 tcaaatcctc cattacagca attgatttag ccggttcgga caaatcctca cgataaagtt
      961 tgtttgcttg tacgatttct tgaagtaatt catcaggaat cctttgcgat aatctaaaag
     1021 aagcctgagg atcatcttta acagaggatg tatttagcca ttgtctcaaa atatcgacga
     1081 caacatcgct ttttagttca ttcaattcta acggttgtag ccgtctccct aaggcatcat
     1141 ctcttttgat atagttttca tattcttttc ttgtagtagc ggtaatcatt tgaagtgatt
     1201 catttgctaa cggttctttt agcgaatcac ccccaatttt attgccactc ccaaaagagc
     1261 taacaacttt atgaaactcg tcaatgaaga ggacaaattc attatctttg tcctcagctt
     1321 ttaaaattcg ctcatattct ttcaatcttg gaattaatgt ttctaaacgt gtttggagga
     1381 catcatctcc cccaacacgc aattgtccga ttgataattt atatacttgt actttctttc
     1441 catcttgtcg ctgtctttca gcccattcac ttgcaagtgc tgttttacct gttccggctt
     1501 cacctaacaa gatagcttgc ggtgtttccg gtctattcaa gatcagtgac aactcatgga
     1561 gttcgtcatc acgtcccact aacttatgtt ttggtggttt cattggtgtg agagcatgat
     1621 taaattcggt ttgaatattc ttaaaaaact cggtactatt ttccatctgt ttgtgttcac
     1681 ttcctctaaa ataagaataa tttacttcta gtcagtcaaa gttaggtact taaatgtcgt
     1741 cttcatgtct ccaaaagaat ctttgttaaa gtcagcattt ttgtattgat caatcaattt
     1801 ttgtgtatca ttgattgctt taatttcggt ctccattcga ctgtctagtt gacgaatact
     1861 ttcttgcgaa tctttatcgt caggattttg ttgtaaacgt tccagatact cagcttttgt
     1921 ggcttcaatt ttcttgatgt tagtatttga agcatttaac tgatcttcta gcttatttac
     1981 aacattattc ttacttgtaa ctgctacaac ttttgaataa tcaatatcgc catctttggt
     2041 aagaaagctt ccttgataga cattagttga atctgaatat ggatttattc tgaaattgat
     2101 attatcagga gtattaacag gttttgcttt tttcccaaaa gaaattactc cttcattatt
     2161 aacatcattc atagaatatt gagaaagtgc ttttgtaact gaatctgctt cattcacatt
     2221 gactttggta tcatcgtcag ctgtatcatc accagaaatt agtgatactt ggtttgcaat
     2281 aaaaacttga taagctcttt cctctaattt accttttagg aataagaaag catccccatc
     2341 agtacttaac atcccatagc tcatttcaag cttcttagga cgatcttttt cttttgttgc
     2401 catatataag ttatagtttt ttccttgcgt agataacaac tttctagcgt tttgggagta
     2461 accaagtttt actactgtta catctctatt ttgatctgtc cacacatctg aaatagtgat
     2521 ttctgctcct gatcgagcaa actccaatga agaccctata ggtgttgtat tgttcacgag
     2581 ataataatta tagtttccta attgataaat gtagctaccc aagcctgtta ataaaaaaag
     2641 cactcctaga aaagttgcaa gcataatgcg taatccgtga cgtgttctct tatccttatc
     2701 accaagcaac cctccaataa tactgtattg atctttggta gatgttgaat tgacgttttt
     2761 ttccatcagt ttgtctgccc cctttcataa aagagcaaac aatcagacgt tttttcggta
     2821 attctaggtt cgggattttg aatcatttcg gtatattctt ccccattaat atttaacaca
     2881 aaaattatct tcctttcttc gttcctttct tttgctcttt attttccaaa taatcaatga
     2941 tttttgctat tgtaccaaga tagtaattcc cctcagcttt gttttttcgg attttgctaa
     3001 ccattggagt aggtacaatc tcttcatctc tcaattcact gaaagatttt ttacctagat
     3061 attttccaat aacagatttt aaaaaacgtt caaaatcttt ctcgtaagtt gttttcttgc
     3121 cttttagttc atggttcata ttgtcaacga cttttgcggt ttcaataatt gttttagttc
     3181 tcagcttaat aatttcatct ggaattttca atcctcgtaa tttcatcgtt gtactaactg
     3241 gtaacccatg ctttactgca tcagcaatac taaaactttt tagatcatct agtaagctct
     3301 ccatatagtt aacaaattca tgccaattaa tagcattcat tgtctcaccc cttcattttt
     3361 ttaaaatgag gttcgggcgt aacacattta tgttattata atatcataat aacataaata
     3421 aatcaatcag caaatttaga tatcccttaa agtatcagca attattcaac aagatattta
     3481 ttttagctat ttcagtcatc ataaaagact aattagcaat aaaataacta atattatcca
     3541 tttatcagac gaaaagaaaa aacttatcta attactctat attaactttt aacaaatagt
     3601 tgacttgtcc aaaaatgtta ccttcaatct cacaaagata ttaagatgtt tcctaccaaa
     3661 actgatatca ccggactttt tattctgtgt tgtccaaaag tgttaccttt agtcgatcaa
     3721 caaattaaaa aaaattgttt ttttcttgac ttgtccaaaa gtgttaccca tagttgttaa
     3781 aatttgtata gattagccat tctttcgaat attatttaga ctgaaacttg cttacaaaaa
     3841 aacttcttgc atttattgtc caaaagtgtt acccatactt ttttaaagaa gcaggttgta
     3901 ttaaaattac aacctgcttc ttctcaatct tcttctaacc aattatgcat tgaaatggtt
     3961 ggcaagtttg attctggctc actaaaagtg aatctgagcc gatcaactct tcttcctttc
     4021 tttgcataga ttttttcata atcaaaagtt tcaaatacct gtattccgtt ttcatcagga
     4081 gctaaaaatt cttctttcgc ctgctctagt attcttttgt caatatttgt cattctataa
     4141 cttgaaggta tacttaatgc tcttctaaaa tcttcaactt tgactaccca ataaccattt
     4201 cgccactctt tatttctgta ttgcatgaga tgacgataca attcttttgt gtagctactt
     4261 cttagttttg taaaattatc aagttcaaaa cgagtaaagt ttttgccaat tgagtttaag
     4321 ataaattcaa attcctcatt tgccttaatt gtaactgttt ccttctgttt atctatccgg
     4381 aatttttgaa aaagccacag ctgttcatag acgtctgaat cttcaaaacg gtaggttaaa
     4441 gtacctagtt tgtcactcaa agcttttaac acagaataaa aactgtcctt ttctcttctg
     4501 tcgtatttga ccaaataacg aaagaggttg aaatcaaaag ttacttctgc tgttccttga
     4561 cgtttcattt ttgataaaat agcccaaaac acatccattt caacaggctt aaatcctcgc
     4621 aaagctgttg tattcagacc attgttataa cgcacaattg catttgtttg ttctgatact
     4681 aaatctacat cactatcaat aatcttttcg gtaacttctt gagacattga ttactctcca
     4741 atcttctttt tgcgctcctt taatcatgtt aatctggtac taattaaagg aaacaatttt
     4801 ttctgcttgt tacctaaatt aaatcacaac taaaggtaac agtcaacaaa gatttgttac
     4861 taatctaatt ttctattttt tcgccttcca aatcaagaaa accgaaggta acgtcttatc
     4921 ctagtgtgat acctcaaatg tccacttttg ttacctttgc tgtccaaaag tgttaccctt
     4981 actgtccact tttgttacct ttactgtcca aaagtgttac ctttataccc ctttaatact
     5041 tactctccca agagattaag cctcccctaa cagatatatt aacagatatt taacagatat
     5101 attaacagat atttaataaa ggaggattac tcctctctta tttcaaaatc ttaaaaataa
     5161 aaaatcttaa aaaaaactaa cactatctag gagtttattt tgcttgtgta actttgaata
     5221 attgaaaaaa aggaaaagga ggaagcttca
//
LOCUS       DAJSBL010000103         5212 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.106, whole genome shotgun sequence.
ACCESSION   DAJSBL010000103 DAJSBL010000000
VERSION     DAJSBL010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5212
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.106"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            604..954
                     /locus_tag="ONT85_002706"
                     /pseudo
     CDS             604..954
                     /locus_tag="ONT85_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C47"
     gene            948..1370
                     /locus_tag="ONT85_002707"
     CDS             948..1370
                     /locus_tag="ONT85_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2379634.1"
                     /translation="MVNKPIALVMGLVLVLFLTSCSKKQEIQKTDLIPFLGTWQTKDH
                     SERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNLLTLRSNK
                     KQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
     gene            2540..3508
                     /locus_tag="ONT85_002708"
     CDS             2540..3508
                     /locus_tag="ONT85_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HCR2379635.1"
                     /translation="MSNEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMRDVGDKKVQ
                     FTFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKID
                     GKSDIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGFA
                     YFSKEDFHELLDIPKSYNQGTIDQRVFNPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKSDDFNKHAPTKEEQNKALELANQQVQSEKAPTSLQEKIKEQKRKLELL
                     QQLEKLEREQNTQNKENNAQKSEINSDIPSELKKDLLDNLQNLFKD"
     gene            3525..4049
                     /locus_tag="ONT85_002709"
     CDS             3525..4049
                     /locus_tag="ONT85_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HCR2379636.1"
                     /translation="MTEKTIKELAEELGVSKTAINKKVSDENRKLWFSKIGNKFVINE
                     TGQKAIKSMFFNENENSNQQLVGEKTKTETHKNANQVPELEVLSLLEQQLNQKDMQLQ
                     EKDEQIKQMQKLLDHQQQLTLQANQQIEKLQEQLQLTYTEESSENKSTTLSEETESIE
                     KQSKTEKKWWQFWQ"
     gene            4425..4706
                     /locus_tag="ONT85_002710"
     CDS             4425..4706
                     /locus_tag="ONT85_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2379637.1"
                     /translation="MDILVRNINPKIIARIDKRCQELTTKTGKKWSRNKYLKVLIESD
                     FDRQLMNYKKDQFDLLLEKFIAIQTENTNILNDYVHTNNQLIETLIGGI"
     gene            4708..5124
                     /locus_tag="ONT85_002711"
     CDS             4708..5124
                     /locus_tag="ONT85_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2379638.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDVNNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIMAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     ENQLNPSRRNQPKNRQKETNQTFTSGNFDPTNFLKR"
BASE COUNT         1967 a          893 c          868 g         1484 t
ORIGIN      
        1 aaaaaaaaga aaaatccgct tatgcaactt cttctttttg tattttgggg cgctacccca
       61 atccccaagt atcggttatt taatagcgcg gaaatccaaa gaataggacg taccctgaaa
      121 caaaaaaaga ctgctctcac gagcagccca aaatgagatt atcctattca tcaacataaa
      181 aatattacaa agtgaagaac tagagaaaga caaaaaaagc atatcaaatc ctctcaaaaa
      241 taacaagtct taaaaaaatg acagataaaa atgcacaaag aattagatat agataaccgc
      301 gaaaaatcaa gctttagaaa gttgctttta gctagaccaa ttaaaaatca caaagtgagc
      361 gtctctatcc attggtagag gggctacttg gcatagtgta cattatgtta actagctcga
      421 taaataacga ttatgttccc aaaacaatat tttatgaact ataacaaaaa tttgttatcg
      481 catattgtat attttaatta tataattata ggtgtatacc acttaaattt aacacaaagg
      541 cgtggttatt ttgaaaccca aaaagaaaat ttatctaggt ctcctctttt ccttgatgat
      601 tggaatagtc aaattgaatc aaaaagtctt aatcaagatc aaataataaa atatgcaaat
      661 tactgtggtg tttatcctaa aaaagtagca cgaatactaa acctttcaga ggtccaaaca
      721 caactaagaa aaggaaatgc catttatatg ggaactgctg gttatggaaa ttatagtaac
      781 agccgtcatg cacttgcatt atttgggtgg gtcaatcaag gtggttatca aacttactat
      841 gtgtggaatc cttggtataa ctacccaact gtaataagtg caaatgcagg accagttaca
      901 attcctgtac ctggaggtgg ttttacttgg gacaatacca ttacaaattg gtaaataaac
      961 ccattgcttt agttatgggt ctagtgttag ttcttttctt aactagttgt tcaaaaaaac
     1021 aagaaataca aaagactgat ctgattcctt ttctaggaac ttggcaaact aaagatcatt
     1081 cagaacgtct agtaatcact aatttaaata cttcaaaaaa tatagaacaa tataccatct
     1141 tagtaaaaga gaacaatcat aaaattcaaa tcattgataa aaatttaaat ataaatgaac
     1201 aagttttcat ttacaaagca acaaataatt tactaacact gcgctctaat aaaaaacaat
     1261 tagattacac ttttagtttg gattctccaa aaaagctcgt atttattttg acaacgaatg
     1321 atgggacaga agggacagct aaaccaattg aattttttaa agctgattga tgaatagttg
     1381 tttgatatta caaaacaata atttcaccga aggtgaaaca agcgaaactt tacaaaaagt
     1441 ggctagcatt tgctagcctt ttttgtattg tggagcgtgg tttttgaggg ggattcaagg
     1501 gggagcttgc gcccctttga caagcggtta tttgcaccaa atacgtaggt atttggctag
     1561 caaatataga gcttgccaaa cactttagtg agttttgtag aaagtacctt taaaggtact
     1621 ttcacattac gaacgatacg tccgtaggac gtactgaaag ggtgggattt atgagcgaac
     1681 taatgcaaaa tttggctagc aaaaaaagtt aaaaaatata tgctaataat cgagcgaaat
     1741 gaacactttt tcaagtgagc gaatccgcca tgacaaagct gaagcagttt atttcagctt
     1801 tgtcatggcg tgtgcctaga cttttaaaaa agtcggcaca cgagcaaata tctttatcaa
     1861 gctctaattt gcgttttaag acgtttgaaa cgtctaagta tataaatata taagagtagg
     1921 tttaagagtg cttaaatcga attttagagg gcattcagac aactttaggg ttgttagggc
     1981 ttgccctgac cgcctgtaag gcgctttaag aacaagataa gggatttata ctaagactgt
     2041 cttaaaacgt ctcaaacgaa ggctcatagc ttctcaaaga gttccttatg aacattttta
     2101 gcaaaggagc cggaagcgac tgcgcagtat aatgtgagag cagagcgatc acaccttttt
     2161 aagcgactgt agggagtaag attcccagtt gagaaaaatg cgcgaatctg tttggagaaa
     2221 aaagaagtgg ttttttaggc gggaaatttt gtccccctct tttataatct ctttttaaac
     2281 ctctttttaa acctctttac cgtcggctat atccttactc tcccaaggga atgacgcttt
     2341 tatctacatc gttttgtctg tttatctaca tcgttttgtc tgtttatcta catcgttttg
     2401 tctgtttatc tacatcgttt tgtctgtttg tacattgaat tagtacatcg acgatgttaa
     2461 tataagatgt agataaacag gcattttgta tttcataacg tgtatctctt gtgcgagtaa
     2521 gaaaggagct attttaaata tgtctaatga aatcgtaaag tatcatcatg aattaaatac
     2581 aattccgttg cgaaaattta caccagtcga aatgaatttg ttcttttcta tcgtatcaag
     2641 aatgcgtgat gtaggtgata aaaaagtaca atttacattc gagcaattaa aagatttaag
     2701 caattataaa gcaactgcaa atgttcgatt catagatgat ttagaaacaa cgtatgacaa
     2761 attaatggat ttacgttttg gtagaagaag tgctgacggt ttgcaacgtg aacgttttgt
     2821 tttatttaat caatttaaaa tcgacggaaa atctgatatt ccttttgctg aaatacaagt
     2881 tcatgaaaaa gccttaccct tacttaataa tcttgaagaa tgggtgcgct attctctcca
     2941 acaattcaat gaattagaaa gcagttattc aaaaacaatg tttcgattac taaaacaata
     3001 cagaaccaaa ggtttcgctt atttttcaaa agaagacttt catgaattac ttgatattcc
     3061 taaaagttac aaccaaggta cgattgatca acgtgtgttc aatccaatac gacaagaact
     3121 aaccgcaatc tttaaaggtt taacaatcaa aaagaaatac ggaaaaggac ggggaaagcc
     3181 tgtcattggc tatcaattta catttaaacc tgagataaaa aaatcagacg actttaacaa
     3241 acatgcccca acaaaagaag aacaaaacaa agcattagaa ctagctaacc aacaagttca
     3301 atctgaaaaa gcacctactt cattacaaga aaaaatcaaa gaacaaaagc gaaaattgga
     3361 acttttacaa cagttagaaa agttagaaag agaacaaaat acccaaaata aagaaaataa
     3421 tgcacaaaaa tcagagataa attccgatat tccttcagaa ctaaaaaaag acttattaga
     3481 caatcttcaa aacttattca aagattgagg ggtttttaga ttttatgaca gaaaaaacaa
     3541 tcaaagaact tgctgaagaa ttaggagtta gtaaaacagc aattaacaaa aaagtttctg
     3601 acgaaaatcg aaaactttgg ttttcgaaaa tcggaaacaa gtttgtgatt aatgaaaccg
     3661 gacaaaaggc tataaaatca atgtttttta acgaaaacga aaactcaaat caacaactag
     3721 ttggcgaaaa aacgaaaacc gaaacccaca aaaacgccaa ccaagttccc gaattagaag
     3781 tcctttctct cctagaacaa cagttaaatc aaaaagatat gcaactacaa gaaaaagacg
     3841 agcaaataaa acaaatgcaa aaattactcg accaccaaca acaactcacc ctgcaagcca
     3901 accaacaaat cgagaaactg caggaacaac tacagttgac ttatacggaa gaatcttcgg
     3961 aaaataaatc aacgactctt tctgaagaaa cagaatcgat agaaaaacaa tcaaagacgg
     4021 agaaaaaatg gtggcaattc tggcaataaa accagcaatt taaattaata aaaattgctg
     4081 gttttattct tctttttgct tttgcgagca gagcaacatg gacccatgtt gcgatatttt
     4141 ggtggcgccc caatccccgt tttttcgttg aatcttactc ggactgcact cgttttacag
     4201 ctgatttttc tgaattttga aagagcataa ggaaacgtgg tatcactttc ttacgaaagg
     4261 tgctagatag gctgtgccta tcacgtttct cagaaaaaaa tttgctatca cttttttgcc
     4321 attgtggcta ggctatgtat agtgttttgg cgaaaaatag ctatacaaat actatatttg
     4381 tatggtatac ataaattata cgctgatacg aaaggaaaat ttccatggac attttagttc
     4441 gaaatatcaa tcccaaaatt attgcaagaa tagataaacg ttgtcaggaa ctcacaacca
     4501 aaacgggcaa aaaatggagt cgaaataaat atctaaaagt tctaatcgaa agtgattttg
     4561 accgtcaact tatgaattac aaaaaagatc aattcgattt attactcgaa aaatttattg
     4621 ccattcaaac agaaaatacg aacatactga atgactatgt tcacacaaac aaccaactca
     4681 ttgaaacact tatcggagga atataacatg agaaaaactt ctgttcaaaa acacgtaacc
     4741 ctttatccca gtcacgttga cgttttagat tccatcatcg aaaaaaatcc agacgtcaac
     4801 aattattcag aagccattcg ttttttatgt atgagttatg aacctgatac caatacaacc
     4861 acaaccagta accaagcaaa actcaatgcc atgggaaaag aaatttctat catgacagaa
     4921 atcatggcag aatttgctga tttatatcta aaagatactg gtgttcttat cggtacaaat
     4981 tctaaagtgt atcaagaagt gaaagaaaaa attgaaaatc aactcaatcc ttcccgacga
     5041 aatcaaccca aaaatcgtca aaaagaaacc aatcaaacct ttacttctgg aaattttgac
     5101 ccaacaaact ttctaaaaag ataaaaaaag aaaccgatcc ctaaaaaaat tcctcgattt
     5161 ctttttaggg atcagtcttc cagcaagatt tgcaagttgg taccaacttg cg
//
LOCUS       DAJSBL010000104         5067 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.107, whole genome shotgun sequence.
ACCESSION   DAJSBL010000104 DAJSBL010000000
VERSION     DAJSBL010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5067)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5067)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5067
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.107"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(406..780)
                     /locus_tag="ONT85_002712"
     CDS             complement(406..780)
                     /locus_tag="ONT85_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCR2379639.1"
                     /translation="MKLNGLQHIGIPTKDFGASKIFYESLGFSLINEEMNVGSKVGFF
                     ELNGTIIEIWEDKTTECRGAIDHLALDTDDIDEVFNSIQDLGYELLDKNIMELPFWEN
                     GIRYFNFYGPNHEIIEISQMKK"
     gene            complement(803..2203)
                     /locus_tag="ONT85_002713"
     CDS             complement(803..2203)
                     /locus_tag="ONT85_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-fructofuranosidase"
                     /protein_id="HCR2379640.1"
                     /translation="MQKIFYKPKDFWFGDCMPFYKDGTFYLFHQRDTRNPKPLTDPFG
                     WSLVTTTDFVKFEEYGEVIHRGGNDDIDQYIYAGSIVQGINEYLALYTGHNRKAKLDG
                     KTSEVLMIASSKDLIHWKKDGEAAKLVPQEGYDKNDWRDPFAIFDQDHGKYVLILGAR
                     KPASKKKPTGRLVYFESEDLKDWNFKGDYWANNEFNMIEMPDIFEIDGTWYLLFSEYC
                     EDKKTKYRVGAGLFSDWKSPVDEAFDGKAYYAARTVGTNETGRFLTGWVATKEGENDQ
                     NNWDWGGALVPHQIIQKTDKTLGVKLPEKIIENFKEITPFSSFKLEKEFGKKERVIVP
                     KIEDYFLFETTLTITSLTKEFALKLFVDSDTQEGYEFRISLINNQLTFGKTPNYPWPQ
                     MFDKGLQRPLFLEIGKEYELKLIVDDTIAVLYIDNVALSTRMYDKVGTALSMTVTEGA
                     IKVDKLDYSINYECGF"
     gene            complement(2207..2617)
                     /locus_tag="ONT85_002714"
     CDS             complement(2207..2617)
                     /locus_tag="ONT85_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCR2379641.1"
                     /translation="MEFIITSHGNIAIGFVDTLKMFFGDNLNCHVVTLGDAGIEEFRE
                     NIEKIVSQVKNQEVMVFADLIGGTPFNEFAKRSEVFQNGFQLLGGMNLGILIEAVNLR
                     LQGKKSDDIIENLRNMNTIACLADMQRHTNEEDE"
     gene            complement(2635..3126)
                     /locus_tag="ONT85_002715"
     CDS             complement(2635..3126)
                     /locus_tag="ONT85_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCR2379642.1"
                     /translation="MDEKMKGIIHVRIDDRMIHGQVATQWTNQLSANRIMVINNEVAN
                     DDVKKAVVRLAAPPNVRTSIITREVAVKNILSGKYEGQKVLIVAVSPIDVKFLIDNGL
                     PITSVNVGNLSRRNGTERIRPTINITEEEREAFKELISDGVEVTVIQTPRDSKEFLKD
                     FIY"
     gene            complement(3136..3927)
                     /locus_tag="ONT85_002716"
     CDS             complement(3136..3927)
                     /locus_tag="ONT85_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HCR2379643.1"
                     /translation="MNKKLTKIDKQVIFRWILNGATSLNYEKMEGLGYAYSMLPFIKD
                     TYKDDPEGMKQSVLAHLQFFNTTPYTAPYIIGMNVGIEKEEKNKSLEAVSALKTGLMG
                     PLAGIGDSVFVVIPWTIFGAIAANMALEGSFVGIILWILASILLRITAYPLYLAGKES
                     GASLLTQIETKLKSITDAVSILGLMVVGALIATVVKVNVGFEFKQGDLTMTGNEIFDQ
                     VMPGLLPACVVAIVYYLLGRKVKPVYVILLILVVSIALRALNILK"
     gene            complement(3920..4693)
                     /locus_tag="ONT85_002717"
     CDS             complement(3920..4693)
                     /locus_tag="ONT85_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCR2379644.1"
                     /translation="MAFWQTFLVVLYGFFINWDKEGPKIGTSQPVVAGFLTGLIMGDI
                     QTGLYIGGTLQLMTLGITSFGGASVPDYQTAALIGTYLAISTNQDASIGITLAIPVAM
                     LIVQLDVLKWSTNIFFQQKAEKFAQKEDYQKLEWMQYLASINTSFVSGIPVLLTVILG
                     PTLVGSAINYIPEWVTGGLKVAGGLLPAVGIGMLLKYLPTKDYFAYLLVGFVLAVYFQ
                     IPILGISLLGAAIALIVYKNRSNNTQVAPAATGGFDEDE"
     gene            complement(4849..>5067)
                     /locus_tag="ONT85_002718"
     CDS             complement(4849..>5067)
                     /locus_tag="ONT85_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCR2379645.1"
                     /translation="RINLFLHLSGMIERIMIGDGITNEHLKEGAQLFDTFLNVSKDFF
                     SEIKDIYCIEIPKGEYELIYTIFEQTIF"
BASE COUNT         1616 a         1028 c          666 g         1757 t
ORIGIN      
        1 ctaggagtga gcatttttcg aaaaatacta aaaatttttc aaaataaaaa aaggcataca
       61 gacaataaac tgtctatacg cctcacagac tctattctat acatctaatt ttggctgtcc
      121 tgcaaaaaat atgtgttagc ctccatcgaa agatcaaact ttggaaaaca tcattataac
      181 tttgcttggt aagagctttg ctaaaacatg atatgacttg tatacaaaat gtggagtgta
      241 ttccaataaa taggaaggat tacaaaattt gaaaccaaat aagtaaaaaa tactgtcact
      301 ttgaattaat ttgaaatagt aaattctcat taaactattg aaatgctgat accaaaaaat
      361 gaagtacttt tcgttcatac caacttagag cacttcgtta tctatttatt tcttcatttg
      421 gcttatctct ataatctcat gatttggtcc gtagaaatta aagtaacgga ttccattttc
      481 ccagaacggt aattccataa tatttttatc taataattca tatcctaaat cttgaattga
      541 attgaatacc tcatctatgt catccgtatc taatgctaga tgatcaatgg ctcctctaca
      601 ctcagtagtt ttatcttccc atatttctat tatcgttcca ttcaactcga aaaagccgac
      661 tttacttccc acattcattt cttcattaat cagactaaac cctaaggatt cataaaagat
      721 ttttgaagct ccaaaatctt ttgttggaat tccaatatgt tgtaaaccat ttaatttcac
      781 ttacatcatc ccttcaattg ttttaaaatc cacattcata atttattgaa tagtctagtt
      841 tatctacctt gattgctccc tccgtaacag tcatacttaa tgctgttccg actttatcat
      901 acattctagt acttaaagca acattatcaa tatataaaac agctatcgta tcatcaacaa
      961 ttaactttag ttcatattct ttgcctattt ccaaaaatag tggtcgttgc agccccttat
     1021 caaacatttg aggccaagga tagttcggag tttttccgaa agtaagctga ttattaatca
     1081 atgaaatacg aaattcgtat ccttcttgag tatcactatc tacaaataat tttaaagcaa
     1141 actcctttgt taacgatgta atagtcaaag ttgtttcaaa tagaaaataa tcttcgattt
     1201 taggaacaat tactctttct ttttttccaa attctttctc taacttaaaa cttgaaaatg
     1261 gagtgatttc tttaaaattc tcaattattt tttcaggcag tttaacacca agagttttgt
     1321 ctgtcttttg aataatttga tgtggaacaa gtgcaccgcc ccaatcccag ttattttgat
     1381 cgttctcccc ttcttttgtt gccacccagc cagttagaaa acggccagtt tcattagttc
     1441 cgactgttct tgctgcataa taggcttttc cgtcgaaagc ttcgtctact ggtgatttcc
     1501 aatctgaaaa taatcctgct ccgactcgat atttagtctt tttatcttca caatactctg
     1561 aaaaaagtaa ataccacgtt ccatcaattt caaatatatc aggcatttca atcatgttaa
     1621 attcattatt agcccagtag tctcctttga aattccaatc ttttaaatct tcggattcaa
     1681 aatagaccaa tctccccgtc ggctttttct tacttgctgg ttttctagct cctagtatca
     1741 aaacgtattt tccatgatct tgatcaaaaa ttgcaaaagg atcgcgccaa tcattcttat
     1801 catatccttc ttgaggaaca agttttgctg cttcgccatc ctttttccaa tgaattaaat
     1861 ctttagaact tgcaatcatt aacacttctg aagtttttcc atccaactta gctttgcgat
     1921 tatgtccagt atacaaagct aaatactcat tgattccttg gacaatactt ccagcgtaaa
     1981 tgtattgatc aatgtcatcg ttgccaccac gatgaattac ttccccgtat tcttcaaatt
     2041 tcacaaaatc agtggttgta actagtgacc acccaaaagg atcagttaaa ggtttaggat
     2101 ttctcgtatc tcgttgatga aatagataaa aagttccatc tttataaaaa ggcatgcaat
     2161 ccccaaacca aaaatcttta ggcttgtaaa aaattttttg catttatcac tcatcctctt
     2221 cgttagtatg tctttgcata tctgcaagac acgcaattgt attcatattt cttagatttt
     2281 cgatgatatc atcgcttttt tttccttgta atctcaaatt gacagcctca atcaaaattc
     2341 ctaaattcat gccaccaaga agttgaaacc cattttgaaa tacttcactt cttttagcaa
     2401 attcgttgaa aggtgttcca ccaatcaagt cagcaaatac cataacttct tgatttttta
     2461 cttgagaaac tattttttca atgttttcac ggaattcttc aatgcctgca tctccaagtg
     2521 ttaccacatg acaatttaaa ttatcgccaa aaaacatttt taatgtatct acaaatccaa
     2581 tggcaatatt tccatgagat gtaattataa actccatcca ctcatcctct gttcttaata
     2641 aataaaatcc tttaaaaatt cttttgaatc tctaggagtt tgtatcactg tcacttcaac
     2701 tccatcacta attagttctt taaaagcctc tctctcttct tcagtaatat ttatcgtcgg
     2761 acggatgcgt tctgttccat tccttcttga taaatttcct acgtttacac ttgttattgg
     2821 taaaccgtta tcaatcaaaa atttaacatc gattggagaa actgcaacaa taagaacttt
     2881 ttgtccttca tatttaccag aaagaatatt tttaactgct acttcccttg taattattga
     2941 agttctaaca ttaggtggtg cagcgaggcg aactacagct ttttttacgt catcattggc
     3001 tacttcatta ttaataacca taattctgtt agcactcaat tgatttgtcc attgtgttgc
     3061 tacttgtcca tgaatcattc gatcatcaat acgaacatgt ataatacctt tcattttttc
     3121 atccatattc aacacctact tcaaaatatt taacgctctt aacgcaatag aaactactaa
     3181 gattaataga attacataaa ctggtttaac ctttcgtcct aacaaataat acacaattgc
     3241 tactacacat gcaggtaata aaccaggcat aacttgatca aagatttcat ttccggtcat
     3301 tgttaagtcg ccttgtttaa attcaaagcc aacattcact tttacaactg tggcaatgag
     3361 tgcaccgacc accattaacc ctaaaattga tactgcatca gtaatagact ttaattttgt
     3421 ttcaatttga gttagtaggc tcgcacccga ttctttccca gcaaggtata aaggataagc
     3481 agtaatcctt aataaaattg atgccagtat ccacaaaata attccaacaa aactaccttc
     3541 caatgccata ttagctgcaa ttgctccaaa aatagtccat ggaataacta caaatacact
     3601 atctccaatt cccgctaaag gacccattag acctgttttc agagcactta ctgcttccaa
     3661 agacttattt ttttcttctt tttcaattcc aacattcata ccaataatgt aaggcgctgt
     3721 atacggcgta gtgttaaaaa attgtaagtg agctaaaacg gattgtttca ttccttcagg
     3781 atcatcttta tatgtgtcct tgataaaagg aagcatagaa tacgcataac ccaacccttc
     3841 cattttttca taatttaatg aagttgcacc attgagaatc caccggaaaa ttacttgttt
     3901 gtcgattttt gtcagtttct tattcatctt cgtcaaaacc tcctgttgca gctggtgcta
     3961 cttgagtatt atttgatcta tttttatata cgattagtgc gattgcagcg ccaagtaacg
     4021 aaatccctaa aattggtatt tggaaataaa ctgccaaaac gaaaccaact aacaaatagg
     4081 caaagtaatc ttttgtaggt aaatatttaa gtagcattcc tatcccaaca gcaggtaata
     4141 gtccccctgc tacctttaaa ccaccggtaa cccattcagg aatataattg attgcacttc
     4201 ccactaacgt tgggcctaat atcacagtta acaaaacagg aattccagat acaaagcttg
     4261 tattaattga tgccaaatat tgcatccact ctaatttttg ataatcttct ttttgtgcga
     4321 atttttctgc cttttgttga aaaaaaatgt ttgttgacca ctttagaaca tctagttgta
     4381 caattaacat tgctaccggt attgctaaag ttattcctat agaagcatcc tggttagttg
     4441 agattgctaa ataagtaccg atcaatgctg ctgtttgata atccggtact gatgcaccac
     4501 caaaactagt aatacctagt gtcattaatt gcaaagtccc accgatataa agtccagttt
     4561 gaatatctcc cataataagt ccggtcaaga aacctgcaac gacaggttgg gaggttccga
     4621 ttttgggccc ttccttatcc cagttaatga aaaatccata caacacaact aaaaatgttt
     4681 gccaaaacgc cattttgttc tctcctttct tcttatactt ttatataagc aattactgtg
     4741 ccaactaaca ttatccaata aagcgggaaa atacagtaaa tattaattaa atgagcgtct
     4801 aaatatactt tggacaccct ttcatttttt tagacgctag tatgctattt agaaaatagt
     4861 ctgctcaaaa attgtataga ttaactcata ctctcctttg ggtatttcaa tacagtaaat
     4921 atctttgatt tctgaaaaaa aatcttttga tacatttaaa aaagtatcaa atagttgcgc
     4981 tccctctttc aaatgttcat ttgttattcc atcaccaatc ataattcgtt caatcattcc
     5041 agaaagatgt agaaatagat taattct
//
LOCUS       DAJSBL010000105         5015 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.108, whole genome shotgun sequence.
ACCESSION   DAJSBL010000105 DAJSBL010000000
VERSION     DAJSBL010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5015
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.108"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            286..1236
                     /locus_tag="ONT85_002719"
     CDS             286..1236
                     /locus_tag="ONT85_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HCR2379646.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVR
                     LTFDQLKDLSNYKATANNRFINDLEMTYDKLMDLRFGKRSANGLDIDRFVMFSEFKIR
                     GTAKDPYVDIQVYEKALPLLNNLDEWVRYSLQQFNELQSSYAKTMFRLLKQYRTKGFA
                     YFSKEDFHELLDIPKSYWNNQANVDNRVIKPIRQELTAIFKGLSIKKKYGKGRGKPVV
                     GYQFTFKPEIKKADDFNKHAPTKEEQNKALALADKQKEYEEPATTINEKIKQKKRELE
                     LLQQIKQLEKEKEALEKQTESALSKKEKKSLLSDLDNLFQ"
     gene            1257..1790
                     /locus_tag="ONT85_002720"
     CDS             1257..1790
                     /locus_tag="ONT85_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HCR2379647.1"
                     /translation="MQPQETFTIKELSELFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLGNQMLLDRLHEYEQDNDKNVNIPSKNVVTNKLLDQLREENEFLRE
                     QLALREQNNSHIQKLLDQQQQLTLQANKQIEKLQEKLQLTYTEEAPENDSTTLSEATE
                     TIDKQIDTEKKWWQLWK"
     gene            1806..1976
                     /locus_tag="ONT85_002721"
     CDS             1806..1976
                     /locus_tag="ONT85_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2379648.1"
                     /translation="MTRKNLLFGSILLGILGFSPMLLSVINKGNKHGELKLNPNYYNS
                     PNEELFVKETDK"
     gene            2426..2959
                     /locus_tag="ONT85_002722"
     CDS             2426..2959
                     /locus_tag="ONT85_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2379649.1"
                     /translation="MKKFTLGVALLTIGAVVLPSTTIVYASENSQVVSLNENNATENV
                     VIEYTEDGMVVTKYEDGYSAEDFANSSDIMTRAAVARWGKKEYTNIAVTTGVAANAIN
                     AAFWAGGKASTSMFGIPGWAIGALLTGASWTKKGASPGNAVAKEWDKNGNGWIGFYYQ
                     RGYDAAGRVVATRYSTL"
     gene            2971..3174
                     /locus_tag="ONT85_002723"
     CDS             2971..3174
                     /locus_tag="ONT85_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2379650.1"
                     /translation="MFLISKSLKSLKTFTKKNPQIATIRIFIFAIAVAIIVNMLSGGQ
                     LYKSGLFLVLILVVLDIIKSKKK"
     gene            3496..3876
                     /locus_tag="ONT85_002724"
     CDS             3496..3876
                     /locus_tag="ONT85_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HCR2379651.1"
                     /translation="MSEQHQNLASDHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVAPD
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            3858..4772
                     /locus_tag="ONT85_002725"
     CDS             3858..4772
                     /locus_tag="ONT85_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HCR2379652.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     TLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPNHQIAIYTHTDKDHIHN
                     HIVINSVNLETGKKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYTQFCDSLKTFGIHCLNRGKNFTYELVHKKK
                     KVRSNKLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTFTP
                     EQSEIRPTRRNHEQHVERGTDHQQELSL"
BASE COUNT         1874 a          880 c          917 g         1344 t
ORIGIN      
        1 tttgtcaccc tcttttataa tctcttttta aacctttttt taaacctctt taccgtcggc
       61 tgtgtcctta ctccttcaat ggatacagcg ttttataaac tacattttgt ctgcttttaa
      121 actacatttt gtctgctttt aaactacatt ttgtctgctt ttaaactaca ttttgtctgc
      181 ttttaaactc tttttttgca ctcacgtatg ttataatatg cattatcaca gacaaaacgt
      241 tgtttataac gtctgtatcc attgaaggag taaggaggaa tatctttggc aaatgaaatc
      301 gtgaaatacc atcatgaatt aaataccatt ccgttgcgga aatttacacc agttgaaatg
      361 aatctatttt tctctattgt gtctagaatg cgcgaaaaag gcgataaaac agtccgtctt
      421 acttttgacc aactaaaaga tttaagtaat tataaagcaa ctgctaataa tcgtttcatt
      481 aatgatttag aaatgactta tgataaatta atggatttac gttttggtaa aagaagtgct
      541 aatgggctcg atatagatag attcgttatg tttagtgagt ttaaaatacg tggaactgct
      601 aaagatccat atgtagacat acaagtttac gaaaaagctt tacctttact aaacaattta
      661 gatgagtggg ttcgctattc actccaacaa tttaacgaat tacaaagtag ctatgcaaag
      721 actatgtttc gattactaaa acaatatcga actaaaggtt ttgcttattt ttctaaagag
      781 gattttcatg aactattaga tattccaaaa agttactgga acaaccaagc aaacgttgat
      841 aatcgtgtta taaagcctat acgacaggaa ttaaccgcaa tcttcaaagg attatcaatc
      901 aaaaagaaat acggaaaagg gcgaggaaaa cctgtcgttg gctatcaatt tacgtttaaa
      961 ccagaaatca aaaaagcaga tgattttaat aaacatgcac caaccaaaga agaacaaaat
     1021 aaagcattgg ctttagctga taaacaaaaa gaatacgaag aacctgctac aacaattaac
     1081 gaaaaaatca aacagaagaa acgagaactt gaactcctcc aacaaatcaa acaactagaa
     1141 aaagagaaag aagctcttga aaaacaaaca gagtccgcac tttcgaaaaa agagaaaaaa
     1201 agtttattat ctgatttaga taatttattc cagtagcaac ctaggaggca accagtatgc
     1261 aaccacaaga aacctttaca ataaaggaat tgtcagaact ttttaagatg agtaggcaag
     1321 caatatcgaa acatatccaa aaattagatt cttccatgat agctaaaaat gagcgtggtt
     1381 ataaggttgt attgcgttct ggagtattgc aacttgctcg caacttaggc aaccaaatgt
     1441 tgctagatag gttgcacgag tacgaacaag acaacgataa aaatgttaat ataccaagca
     1501 aaaacgtggt tacaaacaag ttgctagatc agttgcgtga agaaaatgaa tttttacgag
     1561 aacaacttgc cctcagagag caaaataatt ctcatataca aaaactactc gaccagcaac
     1621 aacaactcac cctacaagcc aacaaacaaa tcgaaaaact gcaagaaaaa ctacagttga
     1681 cttatacaga agaagctcca gagaatgact caacgactct ttctgaagca acagaaacaa
     1741 tagacaaaca aattgatact gaaaaaaaat ggtggcaact ttggaaataa ctggagggaa
     1801 cattcatgac aagaaaaaat cttctattcg gttctatttt actaggaatt ctaggattca
     1861 gtccgatgtt attgtcagtg atcaataaag ggaataaaca tggtgaattg aaattgaatc
     1921 caaattatta caacagtcct aacgaagaat tgtttgtaaa agaaacagat aaataataaa
     1981 aaagactgct ctttcgagca gccagttaaa aaataatgag tgtttattct tgaaaaagca
     2041 acgcgcttat gaagcgctac caaaaattta tcatatttta ttaaaaataa caacccagta
     2101 aaaaatgact ggtaaaaatt cacaaagaaa cggaaagaag caaccgcaga aaatcaagct
     2161 taaaaagagc ttttgaccta gaccaatcga aaatcacaaa gtgagcgcct gtatctattg
     2221 ctacaggcgc tacttggcat agtgtacatt atgtcaacaa gaatttaata taacatattt
     2281 tcctaatgca ttctaactat accacattat caaaaatata aattatcttt actaaataaa
     2341 atattgacaa gcctatacct tttgttttat gattttcaca aagaaaacgt tttctatata
     2401 tttttctgta tcaggaggaa attctatgaa aaaatttact ttaggggtag ctttgttaac
     2461 tattggggca gttgttttac cgtcaactac tatagtttat gcttcagaaa attcccaagt
     2521 ggtatcatta aacgaaaaca atgctactga aaatgtagtt attgagtaca ctgaagatgg
     2581 tatggtcgta actaaatatg aggatggtta tagtgcggaa gattttgcaa attcttcaga
     2641 tatcatgaca agagctgcag ttgcaagatg gggcaaaaag gaatatacaa atattgctgt
     2701 aaccactgga gtagctgcta atgctattaa tgctgccttt tgggcaggag ggaaagctag
     2761 cacttctatg tttggtatcc caggttgggc aattggtgct ttgttaacag gtgcaagttg
     2821 gactaaaaaa ggtgcttctc cagggaatgc agttgcaaaa gaatgggaca aaaatggtaa
     2881 tggctggatt ggattttatt accaaagggg atatgatgca gctggaagag ttgtagctac
     2941 tagatattca acgttataag ttaaggagtg atgtttttga tatctaaatc attaaaatca
     3001 ttaaaaacat ttactaaaaa gaatccgcaa atagctacta ttcgtatttt tatatttgct
     3061 atagctgttg ctataattgt taatatgcta agcggagggc aattatacaa atctggttta
     3121 tttttagtgt taatcttagt tgtcttagac ataataaagt ctaaaaaaaa ataaatcgat
     3181 acattttaga agaatagggt tgtttgaata ttacaaagca acaatttcac cttcggtgaa
     3241 ataagcgaaa ctttacaaaa agtggctagc aaatgctagc ctttttttgt attgtggagc
     3301 gtggattttg agggggattc aagggggagc ttgcgctcct ttgacaagcg gttatttgca
     3361 ccaaatacgg aggtatttgg ctagcaaata tagagcttgc caaacccttt tagtgagctt
     3421 tactcattgt acctttttag gtacaatggt attgcgaacg atacgtccgt aggacgtact
     3481 gaaagggtgg gacttatgag cgaacaacat caaaatttgg ctagcgatca ttctaaaaaa
     3541 tatacctatc gatccgagcc aaaacaaatc agttttcgag tgagcgaatc cgaatttgca
     3601 aagctgaagc agtcagctga agctttgcaa atgagtgtgc cggctttcgt gaaagccaag
     3661 gcacaaaacg cgcgtctggt gacaccgaaa gtggcgccag acattgccca ggcaatcgcg
     3721 cgcgatttag ccaaagctgg agggaatatc aatcaaattg ccaagtggtg caatacacac
     3781 caacacgacg ttgctcctga tgatgctcaa cgtttatccg aaaacctaaa aatcatgcaa
     3841 aaggagctac aaaagatatg gcaacagtta aagtaagtcg aaccacttca tgtagccgag
     3901 caatcaatta cgcagaacca cgagcaaccg tcaaaacagg aataaactgt gacattgatt
     3961 atgccaaaag tgaaatgaaa caaatacgca cgctttatgg aaaagacgat cgtgtacaag
     4021 cacacttatt gattcagtcc tttcgaccag gagaaattac cgctgaaaaa gccaatcaac
     4081 taggggcaga gtatgcgaaa aaaatagccc ctaaccacca aatcgcgatt tacacacata
     4141 cagacaaaga ccatattcat aatcacattg tcattaactc cgttaatctt gaaactggga
     4201 aaaaatttca agcccatggg caagcatttt taaacaaatg ttatgaccta aatgacgaga
     4261 tttgtcttga acatgactta acaatcacgg aacgaggaaa gaaacccgaa aaacgaacca
     4321 tgtctgaaat taaattaaaa gaaaaaaatg aaccggtttg gaaagacgaa attcggtttg
     4381 cgattgatca aacaatgaaa aacaaaaaaa ctcgaacgta tacccaattt tgtgattcac
     4441 ttaaaacatt cggcattcat tgcttgaatc gcggaaaaaa ttttacttac gaattggtcc
     4501 acaaaaagaa aaaagtacga tccaacaaac taggaaagga ttacgaaaag gagacgattt
     4561 tacatgagct ggacagacga gaacgaacct acaatcaaca acgaaccaac gagctacgaa
     4621 gagctcgagc tagcattcgc gaaactgcag aagaagtacg aagagaacaa cgaactttta
     4681 cgcctgaaca atcggaaatt agaccgactc gaagaaacca tgaacaacac gttgaacgag
     4741 gaacagacca ccaacaagaa ttatcgctct aaaacgttaa aagacatcga aaacgaaata
     4801 aagagacaac gtgcctttct tgcgccttat acccaagaaa ggatacaaac tacgatggat
     4861 tcacaagtca aaaaactcca ccaagcgcta ttagaccagc gaaaccaaga acaaaaaaag
     4921 gctttaattc aacgatcgat ggatattctt gcccccttat gttgcttagt actaacatta
     4981 ttagcctttt ggttggtatg gggagaataa acaga
//
LOCUS       DAJSBL010000106         4886 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.109, whole genome shotgun sequence.
ACCESSION   DAJSBL010000106 DAJSBL010000000
VERSION     DAJSBL010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4886
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.109"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(6..284)
                     /locus_tag="ONT85_002726"
     CDS             complement(6..284)
                     /locus_tag="ONT85_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2379653.1"
                     /translation="MKEEQTTNKNYRSKTLQDIENEIKKQRAFLAPYTKERIQTTIDS
                     QVKKFHHALLNQRNKEQKKVIMQRSMDILAPLCCLIMTLLAFWLVWGE"
     gene            complement(244..1158)
                     /locus_tag="ONT85_002727"
     CDS             complement(244..1158)
                     /locus_tag="ONT85_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HCR2379654.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            complement(1140..1520)
                     /locus_tag="ONT85_002728"
     CDS             complement(1140..1520)
                     /locus_tag="ONT85_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HCR2379655.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            complement(1970..2257)
                     /locus_tag="ONT85_002729"
     CDS             complement(1970..2257)
                     /locus_tag="ONT85_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HCR2379656.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
     gene            complement(2277..2501)
                     /locus_tag="ONT85_002730"
     CDS             complement(2277..2501)
                     /locus_tag="ONT85_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin hiracin-JM79"
                     /protein_id="HCR2379657.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            complement(2956..3126)
                     /locus_tag="ONT85_002731"
     CDS             complement(2956..3126)
                     /locus_tag="ONT85_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2379658.1"
                     /translation="MTKKHLLFGSILLGILSFSPMLLSVANKGNKKGELKLNPNYYNS
                     QNEEVFVEKLDK"
     gene            complement(3141..3674)
                     /locus_tag="ONT85_002732"
     CDS             complement(3141..3674)
                     /locus_tag="ONT85_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HCR2379659.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            complement(3695..4639)
                     /locus_tag="ONT85_002733"
     CDS             complement(3695..4639)
                     /locus_tag="ONT85_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HCR2379660.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVR
                     FTFDQLKDLSNYKATANVRFVEDLKATYEKILSLRFGRRSESGLSYDMFVMFTEFKIN
                     GDVENPYVDIRIYEKALPLLNNLDEWVRYSLQQFNELQSGYAKTMFRLLKQYRTKGFA
                     YFSKEDFLELLDIPKSYKQPDIDKRVIKPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKADDFNKHAPTKEDQNKALALADKQKEYEEPATTINEKIKQKKRELELL
                     QQIKQLEKEKKALEKQTESALSEKEKKSLLSDLDNLFQ"
BASE COUNT         1270 a          895 c          831 g         1890 t
ORIGIN      
        1 tctatttatt ctccccatac cagccaaaag gctaacagtg tcatgattaa gcaacataag
       61 ggggcaagaa tatccattga tcgctgcata attacttttt tctgttcttt atttcgctgg
      121 tttaatagcg catggtggaa ttttttgact tgtgaatcaa ttgtagtttg tattctttct
      181 ttggtataag gtgcaagaaa agcacgttgt ttctttattt cattttctat gtcttgtaac
      241 gttttagaac gataattctt gttggtagtc tgctcctctt tcaacgtgtt ggtcatggtt
      301 tcttcgagtc ggtctaattt ccggttgttc agtcgtaaga gttcgttgtt ctcttcgtac
      361 ttcttttgca gtttcgcgaa tgctagctcg agctcttcgt agctcgttgg ttcgttgttg
      421 attgtaggct cgttctcgtc tgtccagctc atgtaaaatc gtctcctttt cgtaatcctt
      481 tcctagtttg ttggatcgta cttttttctt tttgtggatc aattcgtaag taaaattttt
      541 cccacgattc aagcaatgaa tgccgaatgt tttaagagaa tcacaaaatt ggttatacgt
      601 tcgagttttt ttgtttttca ttgtttgatc aatcgcaaac cgaatttcgt ctttccaaac
      661 aggttcattt ttttctttta atttgatttc agacatggtt cgtttttcgg gtttcttccc
      721 tcgttcagtg attgttaagt catgttcaag acaaatctcg tcatttaggt cataacattt
      781 gtttaaaaat gcttgaccat gggcttgaaa tttgttccca gtttccagat tgacggagtt
      841 aatgacaatg tggttatgaa tatggtcttt gtctgtatgt gtgtaaatcg cgatttggtg
      901 gtcaggggct atttttttcg catactctgc ccctagttga ttggcttttt cagcggtaat
      961 ttctcctggt cgaaaagact ggatcaataa gtgtgcttgt acacgatcgt cttttccata
     1021 cagcatgcga atttgtttca tttcgctttt ggcataatca atgtcacagt ttatccctgt
     1081 tttgacggtt gctcgtggtt cggcgtaatt gatcgctcgg ctacaagaag tggttcggct
     1141 tactttaact gttgccatat cttttgtagc tccttttgca tgatttttag gttttcggat
     1201 aagcgttggg cgtcgccagg atcaacgtcg tgttggtgtg tgttgcacca cttggcaatt
     1261 tgattgatat tcccgccagc tttggctaaa tcgcgcgcga ttgcctgagc aatgtctggc
     1321 gccactttcg gtgtcaccag acgcgcgttt tgtgccttgg ctttcacgaa agccggcaca
     1381 ctcatttgca aagcttcagc tgactgcttc agctttgcaa attcggattc gctcactcga
     1441 aaactgattt gttttggctc ggatcgatag gtatattttt tagaatgatt gctagccaaa
     1501 tattgatttt gttcgctcat aagtcccacc ctttcagtac gtcctacgga cgtatcgttc
     1561 gcaataccat tgtacctaaa aaggtacaat aagtaaagct cactaaagct cactaaaggg
     1621 cttggcaagc tctatatttg ctagccaaat acctacgtat ttggtgcaaa taaccgcttg
     1681 tcaaaggggc gcaagctccc ccttgaatcc ccctcccccc cactctccac aatacaaaaa
     1741 agactagcaa atgctagcca ctttttgtaa agtttcgctt gtttcacctt cggtgaaatt
     1801 gttgcaaatc aataattagg tagagctaca tttttttcgt tgttcaaaga ttcttatgcc
     1861 atttactatt aataaatcta taaatatgat aatttttcgg ctctataatt tatgggactg
     1921 atgaatcaga attgattctt cagtcccatt ttcattttat tccaaagaat taactccaaa
     1981 taccaataga agcccatcct ctatatactt gagattcctt actaacaatt tcttgtaatt
     2041 ctattaaaga tttaggtaat ccaaatttat gttttaaaat atatcttcca agaaaactac
     2101 ttaatttcat actaacttca ccaacacttt ttccctcact gatttcttta gaataaagaa
     2161 agagcttttc ttttaagtca ggatagtcat ctatagttgc ttcattgtat actttactaa
     2221 ttgcatttaa aagtttttct tctttagtaa aatccattat aataacctcc ctaccactac
     2281 cttctaggtg cccatggacc atgattaacc caaccattaa caataatttt tccaatttct
     2341 cctttagctt gattccaatc tacccaacat ttttctttgt tacaataaag accatttcca
     2401 tagtaagtag ctgcatcaac ttttattcct gtaccgatgc cagctagaca agttcctaat
     2461 attcctagaa taacacaatg ttttaatact ttctttttca tgattgtttt tccttttcgt
     2521 ttttttaaaa ttgcttgtta tattaaataa tcttagtaat tttattatat aacattttat
     2581 tatataacaa tgattaacgt cataaaaagt agttttgaat tcataatcgt ttaaataaaa
     2641 ttataagatc agttatttaa acagctagta gacataatgt gtatactacc aagtaacgcc
     2701 tgtaccaagg gatccaggcg ctgattttgt gaatttttat tggtctaggt taaaagctct
     2761 ttttaaggct tggttttctg cggttgtttc tttctaattc tttgtgaata ttgatcggtc
     2821 attttttaaa tacttgttat ttttgtttgt gtttgttatg ctttttacat atctcatatt
     2881 catttattat tcttttctaa ggttaataat aggataatct ctttcagaaa gactgctcgt
     2941 acgagcagtc ttttcttatt tatcaagttt ttctacaaat acttcctcat tttgactgtt
     3001 gtagtaatta ggattgagtt ttagttcccc ttttttattt cctttgttgg ccactgataa
     3061 taacattggg ctaaagctta atattcctag taggatagaa ccgaatagca agtgtttttt
     3121 tgtcataagt atttccttct tcatttccat agctgccacc attttttcca attctttgtt
     3181 tgttcgtcca ttgtttctgt tgcttcagaa agagtcgttg aactattctc tgaagactct
     3241 tccgtataag tcaactgcag ttgctcctga agtttttcga tttgttggtt ggcttgcagg
     3301 gtgagttgtt gttgctggtc gagtagtttt tgtatatgag aattattttg ctctttgagg
     3361 gcaagttgtt ctcgtaaaaa ttcattttct tcacgcaact gatctagcaa cttgtttgca
     3421 accacgtttt tgtttggtat ataagcattt ttatcgttgt tttgttcatg tttatgcaac
     3481 ttatctagca acctttgatt gtctaagttg cgagcaagtt gcaatactcc agaacgcaat
     3541 acaaccttat aaccacgctc atttttagct atcatggaag aatctaattt ttggatatgt
     3601 ttcgatattg cttgcctact catcttaaaa agatctgaca attcctttat tgtaaaggtt
     3661 tcttgtggtt gcatactggt tgcctcctag gttgctactg gaataaatta tctaaatcag
     3721 ataataaact ttttttctct ttttccgaaa gtgcggactc tgtttgtttt tcaagagctt
     3781 ttttctcttt ttctagttgt ttgatttgtt ggaggagttc aagttctcgt ttcttctgtt
     3841 tgattttttc gttaattgtt gtagcgggtt cttcgtattc tttttgttta tcagctaaag
     3901 ccaatgcttt attttggtct tctttggttg gtgcatgttt gttaaagtcg tctgcttttt
     3961 tgatctcagg tttgaacgta aattgatagc caataacagg ttttccccgt ccttttccgt
     4021 atttcttttt gattgttagt cctttgaaga ttgcagttag ttcttgacgt ataggtttta
     4081 taactcgttt atctatatca ggttgcttat aacttttagg aatatctaat aattcaagaa
     4141 agtcttcttt ggagaaatat gcgaaacctt tggttctgta ttgctttaac agacgaaaca
     4201 tagtctttgc atagccactt tgtagctcgt tgaattgttg taaagaatag cgtacccact
     4261 cgtctaaatt atttaacaaa ggcagtgctt tttcgtaaat tcgtatatct acgtaaggat
     4321 tttctacgtc cccgttgatt ttaaattctg taaacattac aaacatatcg taagaaagcc
     4381 cagactcact tctacgacca aagcgtaaag ataagatttt ttcataagtt gcttttaaat
     4441 cttctacaaa tcgtacattt gcagttgctt tgtagttact taaatctttt agttggtcaa
     4501 acgtaaaacg aactgtctta tccccttttt cacgcattct agacacgatg gaaaaaaaca
     4561 aattcatttc aaccggtgta aattttctta aaggaattgt atttaattca tggtggtatt
     4621 ttacaatctc attagccata taatcacctc aatccaatta tgaatgacat tttttaaatg
     4681 tcaagacatt catatttgac gttcaaacag acaaaatgtc cttcataaac agacaaaatg
     4741 tccttcataa acagacaaaa tgtccttcat aaacagacaa aatgtccttc ataacattct
     4801 gtatccattg gtagagtaag gacacagctt acggtaaaga ggtttaaaaa gaggtttaaa
     4861 aagagatata aaaagagggg ggctaa
//
LOCUS       DAJSBL010000107         4846 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.110, whole genome shotgun sequence.
ACCESSION   DAJSBL010000107 DAJSBL010000000
VERSION     DAJSBL010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4846
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.110"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(163..1242)
                     /locus_tag="ONT85_002734"
     CDS             complement(163..1242)
                     /locus_tag="ONT85_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006908283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HCR2379661.1"
                     /translation="MTQNHDNTVSRKGKHLTYSERCQIAILKKENYSNRQVADVLGCV
                     PQTINNEIKRGTVTQLNRQNQNGKVYDYYHDVYDADAGQAAYEKNRLNCGRRPLWADT
                     DAFIEWADDMMLLEKWSPDVVVGFARKHELFDPAIIPGTTTLYQWIDRGIMKTKNMDL
                     LEKLSRKPKSSRHGNRPNKRVLGKSIEERPEAVGTRKTFGHWEVDTVVGNKTKSDAVL
                     LTLVERQTRFEVILKLAGKDAESVNQAVRQLRERAGAYFGQLFKTVTSDNGSEFAGLH
                     DALQDVANIYFSHPYASWERGTSENQHKLIRRFIPKGSPIGNVSENQILRIQRWMNDY
                     PRKILGYATPHDTFVQAFRQERLTA"
     gene            complement(1349..2884)
                     /locus_tag="ONT85_002735"
                     /pseudo
     CDS             complement(1349..2884)
                     /locus_tag="ONT85_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323457.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
     gene            complement(2903..3307)
                     /locus_tag="ONT85_002736"
     CDS             complement(2903..3307)
                     /locus_tag="ONT85_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit M
                     N-terminal domain-containing protein"
                     /protein_id="HCR2379662.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAISPEYLFNVLA
                     DQAKQATFQLNDLNKAFLASHIYGKILSAKFE"
     gene            complement(3539..3796)
                     /locus_tag="ONT85_002737"
     CDS             complement(3539..3796)
                     /locus_tag="ONT85_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HCR2379663.1"
                     /translation="MEDSPELFPMLKDRLNELTEKRRQLQIRENEILGIFQQQGEPIQ
                     VKDVQQILTSLDCFLIQSEKKQVKALYHTFIEKITFDPNQK"
     gene            complement(3921..4253)
                     /locus_tag="ONT85_002738"
     CDS             complement(3921..4253)
                     /locus_tag="ONT85_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2379664.1"
                     /translation="MPLDLSTGRKIRFSEIQYEGIGFTLIDLKNSTFEIKEGLDLKNL
                     LCADDKIKKIQLYKVPILTPTVLTMLEMENNINYIDSGSDLLIRWQVITNFLMELKKY
                     SNTQTKFS"
BASE COUNT         1344 a         1013 c          841 g         1648 t
ORIGIN      
        1 catggtgacc cggttgctcg ttaccaccaa ccgtctaatt tttctaatcg agtgggccct
       61 tgacaatgag ctgaggcatg gtagtctccc gactggctgg ccccggccag ctttccagac
      121 acaagccatg ccgagggagg ctcgttgtca agggcccacg tgtcaagccg tcaaccgttc
      181 ctgcctaaat gcctggacga acgtgtcatg cggcgttgca tagcccaaaa tctttcgcgg
      241 gtaatcgttc atccaacgct gaatccgcaa gatttggttt tcgctcacgt tgccgattgg
      301 gctgcccttg gggatgaaac ggcgaatcag cttgtgttgg ttttcgctcg tcccgcgctc
      361 ccaggaagca taggggtggc tgaagtagat gtttgccaca tcctgcaagg catcgtgcag
      421 tcccgcgaac tccgagccgt tgtccgaggt aaccgtcttg aacagctggc cgaagtacgc
      481 accagcccgt tctctcagct gtcggaccgc ttggttgacc gattcggcat ccttcccggc
      541 cagcttcaga atgacctcga agcgtgtctg ccgttccacc aacgtcagca gcacggcatc
      601 cgacttggtc ttgtttccta cgaccgtgtc gacttcccaa tggccgaagg ttttccgggt
      661 accaaccgcc tccgggcgct cttcaatgga tttccctagc acccgtttat tcgggcggtt
      721 gccatgacgg gacgatttcg gtttgcggga cagtttttct agcaggtcca tattcttcgt
      781 tttcatgatg ccacggtcga tccactgata gagggtggtg gtcccaggga taatggccgg
      841 gtcgaacaat tcgtgttttc tggcaaagcc caccaccacg tccggggacc atttttccaa
      901 cagcatcatg tcgtccgccc attcaatgaa ggcgtccgtg tcggcccata agggccgtct
      961 gccgcagttc aagcggttct tctcgtaggc cgcctgcccg gcatcggcgt cgtagacgtc
     1021 atgataatag tcgtagacct tcccgttctg attctggcgg ttaagctgag taacggtccc
     1081 gcgtttgatt tcgttgttta tggtctgcgg cacgcagccc aggacatccg caacctgacg
     1141 gttggaatag ttttcctttt tcaggatggc gatttggcaa cgttctgagt aggttaggtg
     1201 ttttcccttg cgtgaaactg tgttatcatg gttttgcgtc atgtgaattc atccttgtct
     1261 attgagagtt gtggtaactt caatataaca tggaattcac gtggcgtttt tttatttcca
     1321 aatggctaag ttaattataa aatccgcctt gaatttttgt taaataaata tcccctattt
     1381 gaataaaacg attgactaat tgaacttctc gactacacca actattaata tcattgatga
     1441 aggaatataa tttatcgtcc gatgttaatt ggatttgata aatccaagaa ttcaaaaaga
     1501 tagacgttat ttgaagataa taatagtcaa attctatttg aaaatctctc tctatttcct
     1561 taacatactg tctaaatcga atctcttgat aggtgggata tgttaaagct gaattatttt
     1621 ggatatcaac attcttttgt ctaaaatatc tatcatattt ctcaaaaagc tgctgcttat
     1681 cagtgtcttt cttccacgtt tgataaagat actctataag ttcgttgata ttaaaaactt
     1741 cgtctacttt ttccaaatca tccatttgaa tatattttaa ttcattctgt cttttcataa
     1801 ccttcatgcc gttgaaattc acccattgtg gtaaatgata catctgaata taaagttctt
     1861 ccaaatcatc taagaaataa aaggcattgg cacaggtcaa ttgaatgata tttttcaaat
     1921 tgccaacatt ttcactaaag cgatagttta ataaaaaatc taataattgt tgagatatca
     1981 gtattttctt tgctagtctt tcttcttctg atttaaaaaa tatcttgatt aactgttttc
     2041 tttcataaag agaacgactg tctaatgttg gaagtgttag ctttacaggt attcttctca
     2101 acaaagttga taatagttta tcctcaggtg gttctgatgt tgcaaatata atccagcaac
     2161 ttgatgtgta ccagttttca ttatcaccca tccgatgaaa tttcctcgta tccataaact
     2221 gaaaaagttt ctcctgacat tcattgctca atgcatgaat ttcatctaaa aataagactc
     2281 cattattagc taattctgct aaaccctttc ttccatcttc ggctcctgta tatgcacctt
     2341 ttacatgccc aaaaaaattg gacaagaaaa gttctggatt attggcatat tcactacaat
     2401 taactgaaat gaaagatgcc ttctcttcta tataattatt ggccaacaaa tccttataca
     2461 cataatttgc taaatagctt tttccagttc cagtctcgcc atgaagtaaa atgggtaagc
     2521 catttggcgg atagtagatt gctgctttaa tttgagcaat tacatttcgc agacttcctt
     2581 gacttccgat aaccctttct attccaaccc taaaagagct atcataccat ttttccgctt
     2641 cctctgaaga gctaaaaatt tgttgatttg gaactttctc cggtaaagaa aagaagatta
     2701 ctggacgagt attgattttt ccaaattctc catcttttac gaattcattc aaatattgag
     2761 agacaatatt acgactaaca tttaattttt cagatatttt ctgtgctgta ttgttccccc
     2821 atttattttt aatctcagag ttctctatga cataattgac aatgcgttct tttaattctt
     2881 ccatcacagc acctcctcga cactattcga attttgccga cagtattttt ccataaatat
     2941 gacttgctaa aaaggctttg tttagatcat tcaattggaa cgttgcttgt tttgcttgat
     3001 ccgctaagac gttgaaaaga tactctggcg aaatcgcata ccccaaaata tcaacgattg
     3061 ttgcaatcag atcttctttt gattcttcat ctgaaagtaa ttcacgatac aacatcgttt
     3121 gtttgcttac tgtgtcatat tcctctaagg attcatctgc taaaagaacc acttgttcca
     3181 acaaacggtc ggataaatat ttatagaaaa tcagccccaa taaataattt ttatattctg
     3241 acgcatccat tttactacgg agattatctg ccgcactaaa taacttctga tttaattctg
     3301 cacccaaaaa gatacactcc tatctcttat ttaaagttgc tatataccct cttattttag
     3361 catactcagt tttagaaaaa gcaaaaaaat aaagaaactt attcttgata acaagctacg
     3421 tgacgtatat ccctaacaca aaaatagcca tatcctttgg tttggatacg gctatttgat
     3481 attactcatt caattatcaa atcacagtag gtgtgaatct catcgggatc ggcaatatct
     3541 acttctgatt aggatcaaag gtgattttct caataaaggt gtgatacaaa gctttaactt
     3601 gcttcttctc actctggata agaaagcaat ccaaactagt caatatctgt tggacatctt
     3661 tgacttgaat cggttcccct tgttgttgaa agatccctaa gatttcattc tctcgaattt
     3721 gtaactgtct tcgtttttca gtcaattcat ttaaacgatc ttttaacatc ggaaacaatt
     3781 cgggactatc ttccaaagca gcttcctgtt tttgctctaa tagttagatt ttgcgtacca
     3841 ttttcttctt ttattttcct tcgattgctg attttaatat agtctaaagg aaatttatat
     3901 ttattgtatt ctttataact tcaagagaac ttcgtttgag tattactgta tttttttaat
     3961 tccataagaa aattagttat tacctgccac ctaatcagca aatctgaacc tgagtcaata
     4021 taattaatat tattttccat ctccaacatt gtaagtactg ttggtgttaa tatagggact
     4081 ttgtacaatt gtattttttt gattttatca tctgcacaaa ggagattttt taaatctaat
     4141 ccttctttta tttcaaaagt tgaatttttt aaatctataa gagtaaaacc aattccttca
     4201 tactgaattt ctgaaaatcg aatttttctc ccagttgata gatcaagggg cagaaaacct
     4261 tttattacat tgtgagtgtc accatcattt acaatgtcat taggaatttt gattttagaa
     4321 ttctgtggag cacctattgg aagataataa ttcattataa ttttgtagtc ttcatgagga
     4381 aataaagtac ttttaggatt gctaagatat tctcgtaccg aatctaagaa tggcttatcc
     4441 attgaatctt catccccatt taaactaaaa aacattccta caattgcttt tgcaactcta
     4501 agtgaattca ttttctttat tgaatgtgtt aaatcagact taccattctt taaacttgta
     4561 ttgttataaa ggaaattttt attgtcttct gcaaatctaa tatattcctc aacatactca
     4621 ctaccaaaaa aactattaca tttactacat aacgtattgg aatggcaaca gtttttttga
     4681 caaattttat aaggtgcaga acttctttcc gtatgctatt ccgattgtcc ttgacaatga
     4741 gcctcctcgg atgtgacgat cttcggcagg agctaacagt taaagttaga ctgcctcgct
     4801 agcgttagca catccgagct cattatcaag gattcgctgc gccaat
//
LOCUS       DAJSBL010000108         4836 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.111, whole genome shotgun sequence.
ACCESSION   DAJSBL010000108 DAJSBL010000000
VERSION     DAJSBL010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4836
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.111"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(57..173)
                     /locus_tag="ONT85_002739"
     CDS             complement(57..173)
                     /locus_tag="ONT85_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HCR2379665.1"
                     /translation="MILKYPENICMLCMPMVMHKNPSDKSIYHWDFYALLGF"
     gene            complement(418..495)
                     /locus_tag="ONT85_002740"
                     /pseudo
     CDS             complement(418..495)
                     /locus_tag="ONT85_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989471.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            complement(649..1284)
                     /locus_tag="ONT85_002741"
     CDS             complement(649..1284)
                     /locus_tag="ONT85_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR2379666.1"
                     /translation="MEGYAMKISHLGNNIQTIRKFRGMKQQELADKIGINMQSLSKIE
                     RGLNYPAYETLEKIMEVLDVTPNELLSGEWKYVNQSEKEVCQFLRAEERLNAELKHGH
                     YDNFFDSEEEWLEYELEKLREYITDYINGKSIEASDLYPIKEFIQHLKFQKLLDRYDD
                     LYSMDMFGESIEGHKYKTPYQVVKMINPNSKEDMELLREVLRNNNFDDEDE"
     gene            1797..2939
                     /locus_tag="ONT85_002742"
     CDS             1797..2939
                     /locus_tag="ONT85_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HCR2379667.1"
                     /translation="MNNLEFALEMKNKRLASGLSQGELASLTHVSRYSINRFENGKAN
                     ASKETQNIILRCLNYYVCDNPFYLLVDYLSVRFPTTDALEVIRKVLGMKADYFIHYEY
                     GYYGYKEHYAYGEIKVMVSDDEHMGVFLELKGAGSRNMEYVLQAQNRDWYSFLNRCLD
                     CGGVIRRFDLAINDMCGLLDIPTLSEKYKNGGAECRCKNYENVQGGKLSGKNRNLAST
                     LYIGSKASTKYFCLYEKQKEQATKKKHTDIINRFEIRLRDKKAVQAVEELLLTYNPHG
                     LVFYLITDFVEFPDYPLWEIFISHDNLPFEMNPVPVNMERTLQWLERQVMPSIVMIEE
                     IDRLTGSNYMKMIDECTHLSEKQEMLVEQMCTDIADVIESEGVFYE"
     gene            2932..3138
                     /locus_tag="ONT85_002743"
     CDS             2932..3138
                     /locus_tag="ONT85_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HCR2379668.1"
                     /translation="MSNAQDIPVWEKYTLTIEEASKYFRIGENKLRRLAEENKDAGWL
                     IMNGNRIQIKRRQFEKVIDKLDAI"
     gene            3217..4410
                     /locus_tag="ONT85_002744"
     CDS             3217..4410
                     /locus_tag="ONT85_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR2379669.1"
                     /translation="MKEKRRDSKGRILHTGESQRTDGKYLYKYVDAFGNTKYVYAWRL
                     TPTDPTPKGKREKPSLRELEQQIRRDIEDGIDSTGKKMTLCQLYAKQNAQRANVKKST
                     QKQREQLMRLLKEDRLGARSIDTIKPSDAKEWALRMKDKGFSYNTINNHKRSLKASFY
                     IAIQDDCVRKNPFDFKLSEVLENDTKEKVALTEEQEQALLSFIKTDNVYHKYYDDVLI
                     LLKTGLRISELCGLTVADIDFQNEVVIIDHQLLKSKEQGYYIETPKTKSGTRQVPLSR
                     ETIQAFQRVMKKRPKAEPFVIDGRGNFLFINHKGKPKVAIDYTMLFVRMVKKYNKNHK
                     DNPLPHITPHTLRHTFCTRLASKNMNPKDLQYIMGHSNISITMNWYAHASIDTAKSEV
                     QRLIA"
BASE COUNT         1637 a          936 c          922 g         1341 t
ORIGIN      
        1 gcatcaacat gagctaaaac tccaatatta ataattttca tgtgattttc ctccattcaa
       61 aagcccaaaa gggcataaaa atcccagtga taaatactct tatcactggg atttttatgc
      121 ataaccatag gcatacaaag catacagata ttctccggat actttagaat cacatgataa
      181 aggtattctt aaactgggta caaaaaacta agccctccta aaaaaggaca tccaattatt
      241 tgttcccact atcaaattga cagtttattt aagaatacct tgccgcatat ttattaactc
      301 cttttaaata gatacttaaa taatagcacg taagagcata tttgtaaagg aatctccaat
      361 tttttatcaa agagagtacg tgattacaaa atagctgtaa taatgtacca atatttgtta
      421 ttctataatc ttccaattac tcccgttctt ttcaagtacc aaatcaaatt gagatacctg
      481 cgttgccttt gcctgattta ccacaaatca gattttatcc ctttcttcct cttgattgcc
      541 cacaacagaa aagaaaaagc cctgtcaaga gctttgccac cattggtggt gctttgcact
      601 cttgatcggg ctttttgttt gctgtatctt ctcgcaagag gaaaaaagtt actcgtcctc
      661 atcatcaaaa ttgttattcc ttaatacttc tcgtaacagt tccatatctt cttttgagtt
      721 cgggtttatc attttcacga cttgataagg tgtcttatac ttatgtccct ctatgctttc
      781 cccaaacata tccatactgt ataaatcatc atagcggtct aataattttt gaaacttcaa
      841 atgctggata aattctttga ttgggtaaag gtcggacgct tcaatgcttt tcccgttgat
      901 atagtctgta atatattccc gtaacttttc taactcatat tccagccatt cttcctcgct
      961 gtcaaagaag ttatcatagt gtccatgttt cagttctgca tttagacgtt cttcggctct
     1021 taaaaactga cagacttcct tttcagattg attgacatac ttccattctc ccgacaacaa
     1081 ttcattgggc gtaacgtcca gcacttccat tatcttttcc agcgtttcat aggcaggata
     1141 gttcaaccct ctttcaatct tggaaaggct ctgcatattg ataccgattt tgtccgcaag
     1201 ttcctgttgt ttcattcccc taaattttct tatggtctgt atattatttc ctaaatggct
     1261 tattttcatt gcataaccct ccatatctcg ttatatcttc gctattataa gtctatcatg
     1321 cttaatatga tttgtaaagg caaaaaaaga tattgacaaa taagcatata agaattacta
     1381 tgttcttgta atcgaataag cacaatagaa tgaaatataa aaacattcaa aaagaaatac
     1441 ctatctgatt aaatccgtag atatgaatat cggttgaaat ggaacttgtt agaaaaggca
     1501 aaacagaact tgctgacaaa gtatttctga cacggtggcg gaagcctctc aggcttaccg
     1561 ccatgacacc ttgtgtcaag gggaagaaat gctttgtcgg ctggcgagcc tgcgagcctt
     1621 gtctgtgccc ccgttatcta acaggggggc actatttcac aaaagacaag tcaaaaacca
     1681 tacaacccca aagctgtacg gagtaatgag gagttttaag aagatagtac acttcacact
     1741 tttttgaaag gcggtttaca ggtatttcaa cgctattgtg ggattggagg aatacaatga
     1801 acaacttaga atttgcactt gaaatgaaga acaaacgtct tgccagcggt ctttcacaag
     1861 gagaacttgc aagtcttacc catgtatcaa gatatagtat caaccgcttt gagaacggaa
     1921 aagccaacgc cagcaaggaa acacaaaata ttatcctgcg ttgtctgaat tactatgtct
     1981 gtgataaccc tttttatctg ctggtggatt atctgtctgt ccgtttccca acgactgacg
     2041 cattggaagt tatccgaaag gtgcttggca tgaaagcaga ttattttatc cattatgaat
     2101 atgggtatta tggctataag gagcattatg cctacggaga aatcaaggtt atggtttctg
     2161 atgatgaaca catgggcgta tttttggaat taaaaggggc aggctcacgc aacatggaat
     2221 acgtcttaca ggcacagaac agggattggt attcattctt aaaccgctgt cttgactgtg
     2281 gcggtgttat ccgacgtttt gacttggcaa tcaacgatat gtgcggtctg ctggatattc
     2341 ccaccttgtc tgaaaaatac aaaaatggcg gtgcggaatg tcgctgtaaa aactatgaaa
     2401 atgtgcaggg tggaaaacta agcggaaaaa accgtaattt agcaagcact ctttatatcg
     2461 gctcaaaggc aagcacaaaa tatttctgcc tgtatgaaaa acaaaaggaa caggcaacta
     2521 aaaagaaaca tacggatatt atcaaccgtt ttgaaattcg tctgcgtgac aaaaaggcag
     2581 tacaggcagt tgaagaactg ttattgacgt ataaccctca tgggctggtg ttttacctca
     2641 ttaccgattt tgtggaattt cccgactatc cattgtggga gatattcatt tcccatgata
     2701 atttaccttt tgaaatgaac cctgtacctg tcaatatgga acgcactctg caatggctgg
     2761 aaagacaggt catgccgtcc attgtgatga tagaagaaat cgacaggctg acaggttcaa
     2821 actatatgaa aatgattgat gaatgtaccc acctttccga aaaacaggaa atgcttgtgg
     2881 agcagatgtg tacggacata gcagacgtaa tcgaaagtga gggggtgttt tatgagtaat
     2941 gcacaggata ttcccgtatg ggaaaaatac acccttacca ttgaagaagc gtcaaagtat
     3001 ttccgtatcg gagaaaacaa gttaagacgc ttagcagagg aaaacaagga cgctggctgg
     3061 ctcattatga atggcaaccg catacagatt aaacgccgac agtttgaaaa ggtcattgat
     3121 aaattggacg caatctaatg caaatgagcc ttgtatgtgt tatgatgaac acaagtcata
     3181 tcaaggctct ttccaacaag gaaaggagca gacaccatga aagaaaaaag acgggatagc
     3241 aaaggacgta tcctgcatac tggagagagc caacgaacag acggaaaata cttatataaa
     3301 tatgtggacg catttggaaa cacaaaatat gtgtatgctt ggagattgac acccacagac
     3361 ccgacaccaa aaggaaaacg ggaaaaaccc tcacttcgtg aactggaaca gcagataaga
     3421 cgggatattg aggacggtat cgacagcaca ggcaagaaaa tgacgctttg ccaactttat
     3481 gccaaacaga acgcacagag ggcaaacgtg aagaaaagca cacagaaaca acgggaacag
     3541 ctcatgcgat tactaaaaga ggacaggtta ggtgctagga gcattgatac gataaaaccc
     3601 tctgacgcta aggaatgggc gttacgcatg aaagataaag gcttttccta taacaccatt
     3661 aacaaccata aacgctcgtt aaaagcgtcg ttctatatcg ccatacaaga cgattgtgta
     3721 aggaaaaacc cttttgattt caagttaagt gaagtcctag aaaatgatac caaagagaaa
     3781 gtcgcattga cagaagaaca ggaacaagcc ctactctcat tcatcaagac ggacaatgtg
     3841 tatcacaagt attatgatga tgtgctgata ctgttaaaga caggacttcg tatctcggaa
     3901 ctgtgcggac tgacagtagc cgatattgat ttccaaaatg aagttgtgat tatcgaccac
     3961 cagttattaa aaagcaagga acagggttat tacattgaaa cgcctaagac aaaaagcgga
     4021 acaaggcaag tgccattaag cagagaaacg atacaggcat ttcaacgggt tatgaagaaa
     4081 cgcccaaagg cagaaccatt tgtgatagac ggacggggca actttctatt tatcaatcat
     4141 aaaggcaagc ccaaagttgc cattgattac actatgcttt ttgtccgtat ggtaaaaaaa
     4201 tacaataaga accataagga caacccattg ccacatatca caccgcatac actacgccat
     4261 acgttctgca caaggctggc aagcaagaac atgaacccga aagatttaca gtatatcatg
     4321 ggacattcaa atatcagtat cacaatgaac tggtatgccc atgcgtccat agataccgca
     4381 aaatcagagg ttcagcgtct aatcgcataa gaagtattta ccacgatttt aaccacgatt
     4441 gacagggaaa atataagaag atagacctag atatgtgagg tttaccacaa aagcaaaatg
     4501 cccgtagagc cgataaaata aggctttacg gacatttaag aagatataaa aagatagtca
     4561 aaaagacata tataatttat acctaattta agtaagtaca aacatcattt taagaatagg
     4621 aaaggacatt tatatactat tctctgtatt tttgatttat aaataaatat gaattgaatc
     4681 caaatcagct atattactat tttataataa ccaactataa aaaacgaagt taggtgaaaa
     4741 actaacttcg atattcataa aagactctat tagttcaact tagcaaaaga tttaaacaaa
     4801 aaaagatgaa ctatttctag ttcatctttt tgcctt
//
LOCUS       DAJSBL010000109         4807 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.112, whole genome shotgun sequence.
ACCESSION   DAJSBL010000109 DAJSBL010000000
VERSION     DAJSBL010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4807
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.112"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..258)
                     /locus_tag="ONT85_002745"
     CDS             complement(<1..258)
                     /locus_tag="ONT85_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2379670.1"
                     /translation="MERQESIREVQEVPTEQAKETQEGRSFSYGKNFSFLRPSIQISV
                     FVLWRIHNNRYSVGSQLFYQEICHALSKKYSQNAYKEALAFL"
     gene            complement(274..924)
                     /locus_tag="ONT85_002746"
     CDS             complement(274..924)
                     /locus_tag="ONT85_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2379671.1"
                     /translation="MKKRFIYVCVGGLFLFGVGSTFFLIHEKLQLTAKEEKLTVEINK
                     FQEEKKGQEDLVTSLKEQLQQVESEVTTLKKANEELKNNDSTEKGNIQTFQKTVTEAF
                     EVLFNYEPDSFQERKNNCAPYLSDKLMKQYFKEGVSYGDSNGVSSELEKCSVYLKSTQ
                     KATIQGLVVVSYRNKIGDSSWNNAMNIFDVTFDPASKKITFMQNLGSSFNQQLLEE"
     gene            complement(935..2107)
                     /locus_tag="ONT85_002747"
     CDS             complement(935..2107)
                     /locus_tag="ONT85_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HCR2379672.1"
                     /translation="MKKKLLLWCGFGCLPTLLLCMFGFLFMILLISSGSEEEEIDCSP
                     TEIVASTISLTNQGMETNGLQIAKEVQKHIPNTTLEGLAGMLGNFQQESSMSPTAIER
                     PNDPLSGHGIAQWTAGRTTALKNFAKERNKEWSDLGVQVEFLIHELNTTEKASQVALQ
                     AKSVEEATEQWQVKFERAGIPMMGNRITFANQWYAKLGKNDSDPVASITLENGANAGS
                     KDLTDYCLETTGTSSSSDSNVLNVAKSLIGYFHYSQPQRAQFGSVENPDKNGYADCSS
                     FVWLVLTKAGYKTPANVGWFTGSMTSDARGSKQYLQEIPESEAKAGDIIIVNLGSGVG
                     NDGHTAVLAEDWHGLSTKVIEQGGMNTNGVGEGQVNLSFGYLLNGGDICFARPIKK"
     gene            complement(2126..2998)
                     /locus_tag="ONT85_002748"
     CDS             complement(2126..2998)
                     /locus_tag="ONT85_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HCR2379673.1"
                     /translation="MKKMILGSLFSATVLLGMVGTSAYADTTSPGDPSVPPTEIIPPT
                     EETPEVPEEPVLPPTDGGEETPEIPEIPETPETPEIPPTEENPDTGGGTETPEVPENP
                     DTGGGEGTPEVPPVEENPGNGGNTETPEKPSNPGGNEGNTTPPTTPPTNNGGTNTTPP
                     VQDVVVKPDGSIGSQQTGGQNVQIETNNASELTHIPTPSTPIETASGEKIVSVVDGVA
                     YKESGNGLTPISDKVEQLPSGNIAVKGSDGKMKVLPKTGEEVSLVATALGGLLASGSG
                     FAFWRKRKATQKMN"
     gene            complement(3020..3940)
                     /locus_tag="ONT85_002749"
     CDS             complement(3020..3940)
                     /locus_tag="ONT85_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2379674.1"
                     /translation="MMMIRFTEVDNMSLLTDKKIALDKAFIQLKRENEELLARAEIGW
                     CTLEIYTENHEIPVFEGSYDFPVEEFHLISLIEQSLTENGQLSEEAQTFIQTLNSELP
                     KFYQVKKDQKNERRPTKIKKEKKTPLSVETAFTQSNGSLQKKKMSFQWLLFGFIVVVS
                     CGLFIMQLSTQREIEQLTKKEHQLQERIAFAEELATKQPQIDTFSRYFITNYYSGEMA
                     EERYRKKLQSFLTEKVAKQISPQKEKVKSILSWEMKKEGKHWQIAYVITLETEKKTQE
                     TKKVSFQLEEQEKNYLVSTLPELDDFEINQ"
     gene            complement(3940..4209)
                     /locus_tag="ONT85_002750"
     CDS             complement(3940..4209)
                     /locus_tag="ONT85_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2379675.1"
                     /translation="MIIVTGIVLLGIGVLFGYHFAYFPKIGEWQQRQAIQKYFQVKGN
                     EYTYYAEDEDSYIVSLNNQEYRVKFSLNNPIQVVYAVEVVEEVFD"
     gene            complement(4206..4586)
                     /locus_tag="ONT85_002751"
     CDS             complement(4206..4586)
                     /locus_tag="ONT85_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HCR2379676.1"
                     /translation="MITNYKKFWQNSFNYKGKATRKEYWLVALMNALIGFGLGLAIGL
                     AGALNASETIIGCLIGVLIVYCFVVLVPSLSLSVRRLRDAGFHWAFIFFYFVPTIGSL
                     VLLVLYCLPTKESIEDKQVQEETR"
BASE COUNT         1259 a         1053 c          792 g         1703 t
ORIGIN      
        1 caaaaaggca agtgcttctt tgtaggcatt ttgactgtat ttttttgata aagcatgaca
       61 aatttcttga taaaatagtt gactgcctac cgaataccga ttattgtgta tccgccacaa
      121 aacgaacaca ctgatttgaa tcgacgggcg taaaaaagaa aaattctttc cataagaaaa
      181 agaacgtcct tcttgggttt cttttgcttg ttctgttggt acttcttgta cctctcttat
      241 tgattcttgt ctctccataa ccttctcctt ttcttattct tctaaaagct gttgattaaa
      301 gctacttccc aaattttgca taaacgtgat tttctttgac gctggatcaa aggttacgtc
      361 aaaaatattc atggcattat tccaggaact atcaccaatt ttattgcgat acgacaccac
      421 aacgagccct tggatagtcg ctttttgtgt gctttttaaa taaacagaac atttctctaa
      481 ttcagaagac accccgtttg aatcaccata ggaaacgcct tctttgaaat attgcttcat
      541 cagtttgtca gaaagatatg gtgcacaatt gttttttcgt tcttgaaaag aatcaggctc
      601 ataattaaac agaacctcga acgcttctgt cactgttttt tgaaaggttt gaatgtttcc
      661 tttttctgtt gaatcattgt ttttcaattc ttcatttgct ttttttaacg tcgtgacctc
      721 gctttctact tgttgaagtt gttctttgag tgacgttaca aggtcttcct gtcctttctt
      781 ctcttcttgg aacttgttta tctccaccgt cagtttctct tcctttgctg ttagctgtag
      841 cttttcatga ataagaaaaa atgtactgcc tacaccaaat aagaacaatc cgcctacaca
      901 aacataaata aatcgtttct tcatttgaca cctcttattt ctttatcggt cgagcaaaac
      961 aaatatcacc accattaagc aaatacccaa aggataaatt gacttgccct tcacctacac
     1021 cgttagtatt cattccccct tgttcgatta ctttcgtaga aagtccatgc caatcttccg
     1081 ctaaaacagc tgtgtgtcca tcatttccca caccagaacc taagttcaca atgataatat
     1141 cgccagcttt tgcctcgctc tcaggtattt cttgtaagta ttgcttactt cctcttgcgt
     1201 cgctcgtcat tgagccggta aaccacccaa cgttcgcagg cgttttatac cctgctttcg
     1261 ttaaaacaag ccacacaaag ctcgaacaat cggcatagcc atttttatct ggattttcga
     1321 cactaccaaa ttgtgcccgt tgcggttgtg aataatgaaa ataaccaatc agactttttg
     1381 ctacgtttaa tacattacta tcggaagaac tgcttgttcc tgttgtttct agacaataat
     1441 cggtcaagtc tttacttcct gcgtttgccc cattttctaa tgtaattgaa gcgacgggat
     1501 cactgtcatt ctttcctagt tttgcgtacc attgattcgc aaaagtaata cgattcccca
     1561 tcattggtat ccctgcacgc tcaaatttca cttgccactg ttctgttgct tcctctacgc
     1621 tttttgcttg taatgcaact tgtgaagctt tttctgttgt gtttagttca tgaatcaaaa
     1681 actcaacttg tacacctaaa tctgaccact ctttgtttcg ctctttggca aaatttttca
     1741 aagcagtcgt tcgccctgcc gtccattgtg caatcccatg cccagataat ggatcgtttg
     1801 gtcgttcaat cgctgtggga ctcatactcg actcttgttg aaaattgcct aacatacccg
     1861 ctagaccctc taatgtcgta tttggaatat gtttttgtac ttcctttgca atttgaagcc
     1921 cgtttgtctc catgccttgg ttcgttaatg agatagtact tgcgacaatt tcggttggac
     1981 tacagtcgat ttcttcctct tcagaaccac tactaatcaa aagtatcata aacagaaaac
     2041 caaacataca tagtaatagt gtgggtagac aaccgaaccc acaccataaa agaagttttt
     2101 tcttcatgtt tgatccttca ttcgcttagt tcattttttg tgttgctttt cttttgcgcc
     2161 aaaaagcaaa accgcttcct gaagccaata atccaccaag tgctgtcgca acgagagaca
     2221 cttcttcgcc agtcttaggc aatactttca tttttccatc agaccctttc accgcaatat
     2281 ttccgcttgg aagttgttcg accttgtctg aaatgggcgt caagccattt cctgattctt
     2341 tataagcgac accatcaacg acggagacaa ttttttctcc tgaagctgtt tcaattggtg
     2401 tacttggtgt tggaatatgg gttaattcag acgcattatt ggtttcaatt tgaacatttt
     2461 gtccacctgt ttgttggctg ccaatacttc catctggttt tactacgacg tcctgtactg
     2521 gaggcgtagt gttcgtcccc ccattattgg taggtggtgt cgttggagga gtcgtatttc
     2581 cttcattgcc tcctggattc gacggctttt ctggtgtctc tgtattacca ccatttcctg
     2641 ggttttcctc aacgggtgga acttcaggtg ttccttcccc tccgccagta tctggatttt
     2701 ctggtacttc aggcgtttct gtgcctccac ctgtatctgg attttcttcc gttggtggaa
     2761 tctctggggt ttcaggagtt tctggtattt cgggaatttc tggggtttct tctcctccat
     2821 ctgttggtgg taaaacaggt tcttcaggaa cttcaggtgt ttcttccgtt ggtggaataa
     2881 tttctgtcgg tggtacactt ggatcacctg gtgacgtagt atctgcatac gcactggttc
     2941 ctaccatacc tagcaataca gttgcactaa ataaacttcc taaaatcatt tttttcatgt
     3001 gtttttcctc ttctcttatt tattgattta tttcaaaatc atctagttcg ggaagcgtgg
     3061 aaactagata atttttttcc tgttcttcca attgaaaaga cacttttttt gtttcttgtg
     3121 tctttttctc ggtttccaat gtaatgacat acgcaatttg ccaatgcttg ccttctttct
     3181 tcatttccca ggataaaata cttttgactt tttccttttg aggactgatt tgtttcgcaa
     3241 ctttttccgt tagaaacgat tgaagctttt tcctataccg ttcttctgcc atttcacccg
     3301 aataataatt cgtaataaaa taacgactaa atgtatcaat ttgtggttgt tttgtcgcaa
     3361 gttcttccgc aaatgcaatg cgttcttgta actggtgctc ctttttcgtt agttgttcaa
     3421 tctctctttg cgttgaaagt tgcataataa aaagaccaca agatacaacg acgataaatc
     3481 caaacaaaag ccattgaaaa gacatttttt tcttctgaag agagccgttg ctttgtgtaa
     3541 acgctgtttc tacagaaagt ggtgttttct tttccttttt gatttttgtt ggtcgacgtt
     3601 cgttcttttg gtctttcttt acttgataaa acttcggtaa ttcggaattt aaggtttgaa
     3661 taaaggtttg tgcttcttca cttaattgcc cattttctgt taaagattgt tcaatcaatg
     3721 aaatcaaatg aaactcttct actggaaaat catacgagcc ttcaaatact ggaatttcat
     3781 ggttttctgt gtagatttcc aaggtacacc agccaatttc cgctcgtgcc aatagctctt
     3841 cattttctct tttcaattga ataaatgcct tatctaatgc aattttttta tctgttagta
     3901 aagacatgtt atcaacttct gtaaatcgaa tcatcatcat tagtcaaaca cttcttccac
     3961 cacttctacc gcatacacca cttgaatagg gttatttaaa gaaaatttca cccgatattc
     4021 ttgattgttc aaactgacga tatagctatc ctcatcttca gcataatagg tatattcgtt
     4081 gcctttgact tgaaaatatt tttggatcgc ttgtctttgt tgccattcgc caatctttgg
     4141 aaaataggcg aaatgatagc caaataaaac gcctatccct aataaaacaa ttcctgttac
     4201 tataatcatc tggtttcctc ctgtacctgt ttatcttcta ttgactcttt tgtcggtaag
     4261 caataaagaa ccagtaacac aagagagcca atcgttggga caaaatagaa aaaaataaat
     4321 gcccaatgaa aacctgcgtc tcgtaaacgt cgaacgctga gagaaagaga cgggacaaga
     4381 actacgaaac aatacacaat caatacgcct atcaaacaac caattattgt ttcagaagca
     4441 ttcaacgctc ctgctagtcc aatcgctaag cctaggccaa acccaatgag ggcattcatt
     4501 aacgcaacga gccaatactc ttttcttgtg gctttccctt tgtaattaaa gctattctgc
     4561 caaaattttt tataatttgt gatcatatct ttgtcctttc taatgtttat ttttgattct
     4621 agtttcttct gttttactaa caatgaaact tgccataaaa aaggtggcca atacaccacc
     4681 agctagaaat ccacttacaa ataacaaaat tttttcctcc tttctaaaaa atttcgccat
     4741 aaaaaggcaa agtatgaaca cgataaatac catgtacata ctttgccttt ttatgcaata
     4801 tatgtta
//
LOCUS       DAJSBL010000110         4754 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.113, whole genome shotgun sequence.
ACCESSION   DAJSBL010000110 DAJSBL010000000
VERSION     DAJSBL010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4754
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.113"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..1131)
                     /locus_tag="ONT85_002752"
     CDS             complement(<1..1131)
                     /locus_tag="ONT85_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HCR2379677.1"
                     /translation="MARKKRNPDAEKLAESILNTYQPESVEDMQDVLKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKKHDNRRNGYGNKTLKTSFGKVNIDVPRDREASFEPELIP
                     NRKRDVSDIEGKVLSMYARGMSQCDIAATVEDIYGFDISHEMISDITDAVLPELEEWQ
                     ARPLAKCYAFLFVDCMYVTLRENYEAKEYAVYTILGYDLKGNKEILGLWLNQTESKNR
                     WMQVFDELKVGGVEDVFFISMDGVSGLKEGAKAIFPSVIVQRCIVHLVRNALRYLPSK
                     DYKEVCRDMKKFYSASSLNAAHAAFDSFQNRWSHYSGAVDVCKRNFSHVEQLFDYGSA
                     IRKIMYTTNAVESIHSSFRKVTKKGVFSNENAQTVDKVHFMDD"
     gene            complement(1261..2637)
                     /locus_tag="ONT85_002753"
     CDS             complement(1261..2637)
                     /locus_tag="ONT85_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR2379678.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGGVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            complement(2637..3293)
                     /locus_tag="ONT85_002754"
     CDS             complement(2637..3293)
                     /locus_tag="ONT85_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR2379679.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQNKGYSNHRKNNISLVFQNYNLIDYLSPIENIR
                     LVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNL
                     DSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE
                     "
     gene            complement(3303..4580)
                     /locus_tag="ONT85_002755"
     CDS             complement(3303..4580)
                     /locus_tag="ONT85_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR2379680.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDEFKNVVSLEATNNTKRNILFSSGVFTIKEGKNIEENDKNSIIVHEEFAK
                     QNNLKLGDEVDLELLDIEKSGKIKSHKFKIIGIFSGKKQETYTGLSSDFSENMVFVDY
                     STSQEILNNSENNEIANKILMYSGSAESTDLALNKLKELKIDESKYFVEKDSNAFEES
                     LESVSGIKYIIKVMTYSIMLGGMVVLSLILILWLRERIYEIGIFLSIGTSKIQIIMQF
                     IFELIFISIPSIISSLFLGNVLLKVIVDGFINSEDSMISGGSLINNSSFMSNITTLGQ
                     SYLILISIIVLSVVFASSLMLIKKPKEILSKIS"
     gene            complement(4599..>4754)
                     /locus_tag="ONT85_002756"
     CDS             complement(4599..>4754)
                     /locus_tag="ONT85_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HCR2379681.1"
                     /translation="DKDKCINCGKCKKVCQMNKDSVENCNHLECIRCGRCKNACPVDA
                     ISYGVRK"
BASE COUNT         1487 a          866 c          571 g         1830 t
ORIGIN      
        1 atcatccata aaatggactt tgtctacagt ctgcgcattc tcattagaga acactccctt
       61 ttttgtgact tttctgaagc tagagtgaat actttcaact gcattagtag tgtacatgat
      121 ttttctaatg gcgctgccat aatcaaagag ttgttcaaca tgagaaaagt ttcgtttgca
      181 tacatctact gcaccagaat aatgagacca ccgattttga aaactgtcga aggcagcgtg
      241 tgcagcgttt aaagaagaag cactgtagaa ttttttcatg tctcgacaga cttccttata
      301 atccttactt gggaggtatc gtaacgcatt tcgaacaaga tgaacaatac agcgctgcac
      361 aatgacagaa ggaaaaattg cttttgcgcc ttctttgaga ccggaaacac cgtccatcga
      421 aatgaaaaaa acatcttcta caccacctac tttcagttca tcaaatactt gcatccagcg
      481 atttttagat tctgtttgat tgagccatag gcctaaaatc tccttgtttc ctttaagatc
      541 atagccaaga atggtgtaca cagcatattc tttagcttca tagttttcgc gtaaagtgac
      601 gtacatacaa tcaacgaata gaaatgcata acacttggct aatggccgag cttgccattc
      661 ttccaattca ggaaggacgg catccgtgat atcagaaatc atttcgtggg aaatatcaaa
      721 gccgtaaata tcttcaacgg ttgcggcgat atcgcattga ctcattcctc gtgcatacat
      781 ggaaagaacc ttaccttcga tgtcggagac atctcgtttt ctattaggaa tcaactcagg
      841 ttcaaaagaa gcctcccggt ctctgggaac atcaatgttt actttaccaa agctcgtttt
      901 aagcgtctta ttgccataac cgtttcgtcg attgtcatgt ttctttggct ctttagaatg
      961 ggcgtcatac cctaaatggt tattcaattc tccttgaagc attttttcaa ataagggacc
     1021 aaacacatct tttaaaacat cttgcatatc ctctacagat tcgggttgat aagtattcaa
     1081 aatggattca gctaacttct cagcatcagg atttcttttc tttctagcca tcgtgtaatc
     1141 acctcttgat cttattttag aaaaataaaa accgtgaagc aaccacccgc ctaggattga
     1201 tggttactta cacggtttat attacactct catttttggt gctaaaaagc accgaaaatt
     1261 ttaattattg tcaattaata attcttttgg attccttctt aatatattga atgaagaaat
     1321 aatcaatgat acaagaagta ctaaactcat aaatataact acataaatca tgaatttagg
     1381 taatacattg atatctaaac ttgataatgt cttgttaaat ccttcagctt ctgccccacc
     1441 acctaattgg ctagatgcag attgtttagc tatttgttta gctatatcac cagtaacctt
     1501 attcaatata ttattcccaa gcttgtcagc tgtatattga gctaagaaat aggaaccaac
     1561 gaatgcgggg attgatataa ataccatctc aattaggaat tgaccaaaaa tttctaattt
     1621 tgatatacct aatgatagta atactgctat ttctttcttt ctagcattca tccataagaa
     1681 taataataat gaaactacaa ctcctgcaaa tattaatgag ccaaaaaata atttatttga
     1741 aattgagtaa atacctgata tagattgttg taatgcaggg taatttgatg aacttttaat
     1801 caaattatat tctctccaat ttatatctaa tttcccaaga tcttttatta cactatcaag
     1861 attcttatca ccttttacaa agaatgttgc atcttggtat acagctgtat cttcggtatt
     1921 accataaact ttagcagctg tatgaatatc agtgattaat gtattttcgt atagttcttg
     1981 tgctgcactt actccaccgc tattatgccc atcgaataga cctttaattt ctacttctac
     2041 tgtttcatct gcacctttct cattatcagc atcaaataaa ttagatttta tttttatctt
     2101 atctccaact ttaaggttat ttttttcagc taaatcttta tgcattaaga ttttattttt
     2161 atcttcattt tctaaatgct ttccttctac taatttatat gcttctgata caaattttgt
     2221 ttcttttgtt gagtcgttaa ccccagttaa cataactgtt cttttaaaat tctttgctct
     2281 ttcaggtgat tgattcgcaa gagtctcttg agtttcaata atatcataat caactaaatc
     2341 tgcaacactg tttattcttt ttacatagga gtctatacta tctgtttgag atatcttttt
     2401 aatatcttca ccttttacat ttcccccacc tctaggtgtt cctggattta cttgtctatt
     2461 tatctccata gaaaaactat ttgttatatt agcaaatgtt tcttttgaag ctctgtccgt
     2521 agcatcttta atagataaac taatgagact taaagttgac atagataaaa taactaatat
     2581 gataattaat gattttaaac tttttctagt tacataagca aatgcatttt ttatcattat
     2641 tcaacctcca aattcatttt atttactttt ttcaactttt taccacttag ttctaaaatg
     2701 atatccgcag aatctgctac ttccttacta tgtgttacaa ctattacaca tttatttcta
     2761 tctttagcta atgtctttaa tatattaatt atttctccag cagtaacact gtctagatta
     2821 ccagtaggct catcagctag tattattgga gcatctgata ccaatgctct agcaatagct
     2881 actctttgtt gctgtccacc agataatttc ataacatttc tttttatttg ttttttatct
     2941 aaacctagtt caaacaagat actttcatct gctgatttat ttactaatct aatattttca
     3001 attggtgata aataatctat taaattatag ttttgaaata ctaaagatat attatttttt
     3061 ctatgattac tatatccttt attttgtata tcttcattct taaacaatat ttttcctgtt
     3121 tgaggtttat caagtccagc aagtaaggaa agaagtgtag attttcctgt tcctgacttt
     3181 cctactatcg cataaaactt cccaagttca aatttttgat ttactcctga caaaactttt
     3241 tctttagaat ttgcataact atatgctaca ttctttattt ctaatatatc cattattttc
     3301 tcctaactta tttttgataa gatttcttta ggcttcttga ttaacattaa tgaagaagca
     3361 aatacaactg ataaaacaat aatacttatt aatattaaat aactttgtcc aagtgttgtt
     3421 atatttgaca taaaactact attatttatt aaacttccac cggaaatcat tgaatcctct
     3481 gagttaatga atccatctac aattactttt agtaatacat ttcctaaaaa taaggaagat
     3541 attatacttg gtatcgatat gaatattaac tcgaatataa attgcattat aatttgtatc
     3601 ttagatgttc caatagataa gaatatacct atttcataaa ttctttccct taaccataga
     3661 atcaagatta atgaaagaac aaccatccca cctaacataa tagaataagt cattactttt
     3721 attatatatt ttattccact cactgactct aaagactctt caaacgcatt agaatctttt
     3781 tcaacaaaat actttgactc atcaatttta agctctttca atttgtttaa ggctaagtct
     3841 gtagattctg cactaccaga atacattaaa attttatttg caatttcatt attttctgaa
     3901 ttatttaata tctcttggct tgttgaataa tctacaaaca ccatattttc actaaaatca
     3961 gaagataatc ctgtatatgt ttcctgtttt ttaccagaaa agatccctat aattttaaat
     4021 ttatgacttt ttatttttcc acttttttca atatctagta attcaagatc aacttcatca
     4081 cctaatttta gattgttttg tttagcaaat tcttcatgaa caataattga attcttatca
     4141 ttttcttcta tatttttacc ttctttaatt gtaaatactc cactactaaa taaaatattt
     4201 cttttagtat tatttgtagc ttcgagtgaa acaacattct taaattcgtc agataaatct
     4261 tctctattta ttctttgttc tccgctaact actttagcat cttttagttt tgctaatcca
     4321 tcatattgaa ttattttttc ttcaatttct tttaattttt caatatcttt aaattgatta
     4381 acattaaaat atttaccatc tttttttgtt attgatattg aagaattaga actttcatat
     4441 aaagcctttt ctatttcatt gcttgatttc attattgtta aacaagaata caagcaagaa
     4501 agaactatag ttaatatgat aaaaattatt aaagttctat ttttctttct tgtaatatat
     4561 gctatagcat tttttatcac tttcctaact actcctctct attttcttac tccataacta
     4621 attgcatcca ctggacaagc atttttacat cgtccacatc tgatacactc cagatgattg
     4681 caattctcaa ctgaatcttt attcatttgg caaacttttt tacattttcc acaattaata
     4741 cacttatcct tatc
//
LOCUS       DAJSBL010000111         4746 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.114, whole genome shotgun sequence.
ACCESSION   DAJSBL010000111 DAJSBL010000000
VERSION     DAJSBL010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4746
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.114"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            463..1482
                     /locus_tag="ONT85_002757"
                     /pseudo
     CDS             463..1482
                     /locus_tag="ONT85_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1499..1648
                     /locus_tag="ONT85_002758"
     CDS             1499..1648
                     /locus_tag="ONT85_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2379682.1"
                     /translation="MKAAYRPIEPYGFEQIIVNDEEHLPEECTEVEPPIPNWKPKFNY
                     WEGNK"
     gene            1645..2091
                     /locus_tag="ONT85_002759"
     CDS             1645..2091
                     /locus_tag="ONT85_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2379683.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGIRADGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYESLENENGDLKQINAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            2093..2230
                     /locus_tag="ONT85_002760"
     CDS             2093..2230
                     /locus_tag="ONT85_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HCR2379684.1"
                     /translation="MFSFSDVKMMYDWGCFTNEQVMVFVPLCITEEKADKIISKEESA
                     S"
     gene            2268..2561
                     /locus_tag="ONT85_002761"
     CDS             2268..2561
                     /locus_tag="ONT85_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2379685.1"
                     /translation="MINLGEWGAIAGSITAIVSLILLVIKPITASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHAEKLVEHTQQIKTLFRERRK"
     gene            2558..2782
                     /locus_tag="ONT85_002762"
     CDS             2558..2782
                     /locus_tag="ONT85_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HCR2379686.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESEEGGNK"
     gene            2779..3804
                     /locus_tag="ONT85_002763"
     CDS             2779..3804
                     /locus_tag="ONT85_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HCR2379687.1"
                     /translation="MKKKITITAMSLLTALFLLPINTFAYTINDEYNLAPNQGDSRLA
                     IPNKIILHETGIDAPARNVAANMKNNYNGSNSYTTDVIGDGGIVYRVGEQGYVSWGAG
                     NANPYAPVQIELQRTYDKALFEKNYRAYIEYTRDSAKKYGIPLTLDQGTSLFTKGIIS
                     HLWVTNYVWGNHTDPYGYLSQMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGA
                     ANPTLTDGTNHAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADG
                     IYRPDVNQAYNTSGNVGYHVSFNMRNFPSKKVYVMMRATNDPEGNTKGGAQDFHDKRW
                     YLNIPKR"
     gene            3994..4067
                     /locus_tag="ONT85_002764"
     tRNA            3994..4067
                     /locus_tag="ONT85_002764"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4028..4030,aa:Ile,seq:cat)
BASE COUNT         1656 a          806 c          953 g         1331 t
ORIGIN      
        1 ggtaagccga atttaatgtc caaactaaaa tcgagcgatt ttaacgttgg ttaccacggg
       61 tcactaactt cggataacga aaagctacat tttacttctg atggtacagg aagcattatt
      121 atgtttacgc gtattaatac acctcagctt gctagtggga aaacctatac tctgagtgcg
      181 aaagttcgat ttgatgaagg aactacagga gctattgata aattacgttt ggtgtatcgt
      241 acatcaccag gagaaaagat attattggaa gcaaatagta caaatattac aacagatgat
      301 gtagggaaag aaataacaat caaaggtaca gctaacgtta attatcaaat cacaaattta
      361 gatcgatttt atatgagtat tagctttgtt gacagggata aaataaatgg cggatttaag
      421 ttgtacgaca tcaaaatcga agaaggctca acagccacac cgtaccagcc taatctactt
      481 gatgcaccgt attatttgag taaggtggct ttgggtgaga atctaattaa accagaatca
      541 caacaaccag ttactaatag taactatctt attaacacct ataacattaa accaatggta
      601 aaaggtaaga agtataccat cacacttgaa ggaactaagc cagcaacaca ggtttttaga
      661 ccatttttca cacgagcaac aggagatgca tgggaggttg gtgacttaca accagtagaa
      721 gggttaactg atgtttggtc tggaacattt acagcagcag ctgacccaca ccctaccagc
      781 ccacaagttc agatttgtca ggtaccaagc acaagtttag gaaactgtac aattaaatgg
      841 ttaaaactag aggaaggcga cacacgaacc ccgaatattg agcaatataa ataccgagga
      901 atcggcatgc gagactcaaa caatccaaaa gattacgtct gggatctagc accagaatat
      961 gtcgaagaca atctggccac agatattaaa atttctgaaa ttactggtaa agcaaacaat
     1021 tataccgatg ggaaagtatc ggagattaat tcgcagttga ctgcttcaat taatgaagta
     1081 gacaccacag ctaaggatgc tcaaacaaaa gcgaatgcta atgcgactgc tatagatgaa
     1141 ttagacaata agatcgatga acgcattaat gatacagcta ctaccacatt aacagttaca
     1201 aacgggaata ccggatcagc aaagctttat cgtgaaggaa aaacagtttc tatatatttt
     1261 gtggctttaa acggaagaag cagtggtgga aatgattcaa cgatactaac aattccagaa
     1321 ggctatcggc caccaattag ttttgagcaa ctggttggct cgatagaccg ttctactttg
     1381 aacagtgctc agttatctat tggtgcagat ggagccatta aatggcgaag aaactcaagt
     1441 tatggatcgg attatacctt tgcaattact tacacgattt agaaaagcgt gaatcgatat
     1501 gaaggcagca tatagaccaa ttgaacctta cggattcgag caaatcattg tgaatgatga
     1561 agaacattta ccggaagaat gcacagaagt cgaaccaccg attccaaatt ggaaaccgaa
     1621 attcaattac tgggagggaa ataaatgaaa aacatttgga aatatggacg tactggcgga
     1681 gagtacgcag gaaaagtatt ggacgacatg cttgtatccg ttccttacac agatcagcca
     1741 ccgcttgaag gaattcgtgc tgatggcgaa ccgttaacaa tcgctgatca aatgtttgat
     1801 cctaaattga atcaatggat tattttagcg aacgcactag atcacaacga tttaaacaat
     1861 ctcaaagcaa tgtatgagtc gttagaaaat gagaacggcg atttaaaaca gatcaatgcc
     1921 aaactcatgc taagcgatgt agcaattaaa caggaaaata ctgcattgaa agaaaaagcg
     1981 gatagtttag cacaaatcaa ttcaaaaatg atgcttgctt cgttacaaaa tagcaaagac
     2041 atttcagaaa ttaaagagca actaaatcca gcttcaaagg gaggtgagta gtatgtttag
     2101 ttttagcgat gtgaaaatga tgtatgattg gggctgtttc actaacgaac aagtaatggt
     2161 tttcgttccg ttgtgcatta ctgaagaaaa agcagataaa atcattagca aagaagagag
     2221 cgcatcttaa ttgatgtgct ttttattttg attcaaggag ttgtcacatg attaatttag
     2281 gggaatgggg agcgatagca ggatcaataa ccgctatcgt ttctttgatt ttattagtaa
     2341 taaaaccaat tactgcatct ttctcgaaga ttactgagac tctttcaaaa gtaagtcaca
     2401 atttagattt gctgactaaa gatttagaat cgagcaaatc agatcgattg atgattcatg
     2461 aagaactaaa gaaacacgat gaaagattag atacacatgc agaaaaattg gtagaacaca
     2521 cgcaacaaat taaaacttta tttagggaga gaagaaaatg aataataaaa cgttcgaagt
     2581 actaaaatgg ttcgcactgg taattattcc cgcactagct actttcgtgg ggttagttgg
     2641 taaagcgctc aattggcagt acacagatat ctgtgttgtc atcattactg gttttggcgc
     2701 gtttttaggg agtgtgttgg gtgtatcaaa tcgaacctac aaaatgttct cggctgaaag
     2761 cgaagaagga ggaaacaaat gaaaaagaaa attactatta ctgcgatgag cctgttaacg
     2821 gctctttttt tattacccat taatacgttt gcttatacta ttaatgacga gtataattta
     2881 gcgccgaatc aaggagactc cagattagca attcctaaca aaattatttt gcacgaaact
     2941 ggaatagatg caccagcaag aaacgtagcc gccaacatga aaaataatta taacggaagc
     3001 aattcttata ctacagatgt tattggtgac ggtgggattg tttaccgtgt gggtgagcaa
     3061 ggatatgttt cgtggggagc tggtaacgct aatccttatg cgcctgtaca gattgaatta
     3121 cagcgcacat atgataaagc attgtttgaa aaaaactatc gagcttatat tgaatataca
     3181 agagatagtg caaaaaaata tggaattcca ttgactcttg atcaaggaac ttctttattt
     3241 acaaaaggaa tcatttctca tttgtgggtg acaaattatg tttgggggaa ccacacagat
     3301 ccatatggtt acttatcgca aatgggagtt agcaaagaaa agcttgctta tgatttagct
     3361 catggattta ccgatgaaaa tccaacaact tctgaaaaca agcctgtcat tgatccaaca
     3421 cgagctggtg cagctaatcc tacgctgaca gatggaacaa atcacgccca cattgatcag
     3481 tttggggaaa tcgaaaacgc aaacttgcat gtggctggat ggcacattgc taactataaa
     3541 tacgagtata ttttcattat ggactacaat actgggaaag aattagctcg agtaagagct
     3601 gatggaattt atagaccaga tgtaaaccaa gcttataata cttctggaaa tgttggttat
     3661 catgtatctt tcaatatgcg taattttcct agtaagaaag tctatgtaat gatgcgggca
     3721 acgaatgatc cagagggaaa cactaaaggc ggtgcgcaag atttccatga caaacgttgg
     3781 tatttaaata ttcctaaacg ataaaaatag ctcctcgttg aggagcagta cataactata
     3841 ttgacaacta taaaaattat tcgataaaat agtgatgtta tcgcatatct tcactatcac
     3901 ccataaatag tcacactcca agctatgcga taacaggttt gttgccacac attctactgg
     3961 ttgattgttt atggctttat gtggcaacaa ccagtaccct tagctcagtt ggtcagagca
     4021 gacggctcat aaccgtccgg tcgtaggttc gagtcctaca gggtacatta acgtagccat
     4081 ttgaatcgtt ctgtgttaga atttttttga agagtattat acaagctaaa gcttttcttc
     4141 attgccactc aaatgagtgg cttttttatg tatcctttta tggattaatg aaaggatgtt
     4201 tcacatagtt atacttctgt atatttgaaa agttttactt tgatttttaa atagaaagac
     4261 atttgggtta aattgtgaga taataataaa gaagagttta aagcgcaccc caaaccactt
     4321 ccccataagt gtgttacgct ttaaactctt ttatatttga agccattaaa aagcatacca
     4381 tatttttgaa aaaaagtgag aaaaaaggct tacaattgga gtggtagtta attagtgact
     4441 tatttttgat tttatagcac tgatactata aaatatagat atcatcatat tacacaatct
     4501 taatactaac ttaaaaaata tctcctttca caagtatggt gataaaattc gttccgggct
     4561 acctttttag gtagcctact ttaatcttta tacctttctg gatcaacgaa agtatacttt
     4621 atatagtcat aacgccgatg atcgctacgt gcgtccggca cgtcagtcat gaatcggcta
     4681 cactagacta gacagaaaaa ataaggtgtg tagaataata aaaaacacac tggaggattt
     4741 ttcatg
//
LOCUS       DAJSBL010000112         4709 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.115, whole genome shotgun sequence.
ACCESSION   DAJSBL010000112 DAJSBL010000000
VERSION     DAJSBL010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4709
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.115"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            105..356
                     /locus_tag="ONT85_002765"
                     /pseudo
     CDS             105..356
                     /locus_tag="ONT85_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            449..1243
                     /locus_tag="ONT85_002766"
                     /note="aph(3')-IIIa"
     CDS             449..1243
                     /locus_tag="ONT85_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HCR2379688.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            1653..1736
                     /locus_tag="ONT85_002767"
                     /pseudo
     CDS             1653..1736
                     /locus_tag="ONT85_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527320.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
     gene            1785..1868
                     /locus_tag="ONT85_002768"
     CDS             1785..1868
                     /locus_tag="ONT85_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuator leader
                     peptide ErmL"
                     /protein_id="HCR2379689.1"
                     /translation="MLVFQMRYVDKTSTVLKQTKNSDYADK"
     gene            1993..2730
                     /gene="erm(B)"
                     /locus_tag="ONT85_002769"
     CDS             1993..2730
                     /gene="erm(B)"
                     /locus_tag="ONT85_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HCR2379690.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(2993..3856)
                     /locus_tag="ONT85_002770"
     CDS             complement(2993..3856)
                     /locus_tag="ONT85_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin zeta"
                     /protein_id="HCR2379691.1"
                     /translation="MANIVNFTDKQFENRLNDNLEELIQGKKAVESPTAFLLGGQPGS
                     GKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELVKLYEKDVVKHVTPYSNRMTEAI
                     ISRLSDQGYNLVIEGTGRTTDVPIQTATMLQAKGYETKMYVMAVPKINSYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLEKELNRKVSGKEIQPTLERIEQKMVLNKHQETPEFKAIQQKLESLQPPTP
                     PIPKTPKLPGI"
     gene            complement(3858..4130)
                     /locus_tag="ONT85_002771"
     CDS             complement(3858..4130)
                     /locus_tag="ONT85_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HCR2379692.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEV
                     NLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYSKQVLNKEKVA"
     gene            complement(4147..4356)
                     /locus_tag="ONT85_002772"
     CDS             complement(4147..4356)
                     /locus_tag="ONT85_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HCR2379693.1"
                     /translation="MGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIETAK
                     NGGNVKEVMDQALEEYIRKYLPDKL"
     gene            complement(4454..>4709)
                     /locus_tag="ONT85_002773"
     CDS             complement(4454..>4709)
                     /locus_tag="ONT85_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="chromosome partitioning protein ParA"
                     /protein_id="HCR2379694.1"
                     /translation="IGFVPYLVDTDSATIKSNLEELYKQHKEDNLVFQNIIKRSNKVS
                     TWSKNGITEHKGYDKKVLSMYKNVFFEMLERIIQLENEKE"
BASE COUNT         1445 a          796 c          865 g         1603 t
ORIGIN      
        1 tcttgggagt tttgagacta ctatatgcgt ttttttgttg atttaccaac atgtttagac
       61 attttcctct aggaaaaaat taactagcac cacgggttat cttttatata gaagatatcg
      121 ccgtatgtaa ggatttcagg gggcaaggca taggcagcgc gcttatcaat atatctatag
      181 aatgggcaaa gcataaaaac ttgcatggac taatgcttga aacccaggac aataacctta
      241 tagcttgtaa attctatcat aattgtggtt tcaaaatcgg ctccgtcgat actatgttat
      301 acgccaactt tgaaaacaac tttgaaaaag ctgttttctg gtatttaagg ttttagaatg
      361 caaggaacag tgaattggag ttcgtcttgt tataattagc ttcttggggt atctttaaat
      421 actgtagaaa agaggaagga aataataaat ggctaaaatg agaatatcac cggaattgaa
      481 aaaactgatc gaaaaatacc gctgcgtaaa agatacggaa ggaatgtctc ctgctaaggt
      541 atataagctg gtgggagaaa atgaaaacct atatttaaaa atgacggaca gccggtataa
      601 agggaccacc tatgatgtgg aacgggaaaa ggacatgatg ctatggctgg aaggaaagct
      661 gcctgttcca aaggtcctgc actttgaacg gcatgatggc tggagcaatc tgctcatgag
      721 tgaggccgat ggcgtccttt gctcggaaga gtatgaagat gaacaaagcc ctgaaaagat
      781 tatcgagctg tatgcggagt gcatcaggct ctttcactcc atcgacatat cggattgtcc
      841 ctatacgaat agcttagaca gccgcttagc cgaattggat tacttactga ataacgatct
      901 ggccgatgtg gattgcgaaa actgggaaga agacactcca tttaaagatc cgcgcgagct
      961 gtatgatttt ttaaagacgg aaaagcccga agaggaactt gtcttttccc acggcgacct
     1021 gggagacagc aacatctttg tgaaagatgg caaagtaagt ggctttattg atcttgggag
     1081 aagcggcagg gcggacaagt ggtatgacat tgccttctgc gtccggtcga tcagggagga
     1141 tatcggggaa gaacagtatg tcgagctatt ttttgactta ctggggatca agcctgattg
     1201 ggagaaaata aaatattata ttttactgga tgaattgttt tagtacctag atttagatgt
     1261 ctaaaaagct ttaactacaa gctttttaga catctaatct tttctgaagt acatccgcaa
     1321 ctgtccatac tctgatgttt tatatctttt ctaaaagttc gctagatagg ggtcccgagc
     1381 gcctacgagg aatttgtatc gataagaaat agatttaaaa atttcgctgt tattttgtac
     1441 atttaacttg acggtgacat ctctctattg tgagttatta gtggtacagt tttcaaccgt
     1501 tttaattata aaaaagtggt gcatttttaa attggcacaa acaggtaacg gttattgcag
     1561 gtgtatttct tatctatggg tttaacatgg attttatcat taaaatcatg agtattgtcc
     1621 gagagtgatt ggtcttgcgt atggttaacc ctaaagttat ggaaataaga cttagaagca
     1681 aacttaagag tgtgttgata gtgcattatc ttaaaatttt gtataatagg aattgaagtt
     1741 aaattagatg ctaaaaattt gtaattaaga aggagggatt cgtcatgttg gtattccaaa
     1801 tgcgttatgt agataaaaca tctactgttt tgaaacagac taaaaacagt gattacgcag
     1861 ataaataaat acgttagatt aattcctacc agtgactaat cttatgactt tttaaacaga
     1921 taactaaaat tacaaacaaa tcgtttaact tctgtatttg tttatagatg taatcacttc
     1981 aggagtgata acatgaacaa aaatataaaa tattctcaaa actttttaac gagtgaaaaa
     2041 gtactcaacc aaataataaa acaattgaat ttaaaagaaa ccgataccgt ttacgaaatt
     2101 ggaacaggta aagggcattt aacgacgaaa ctggctaaaa taagtaaaca ggtaacgtct
     2161 attgaattag acagtcatct attcaactta tcgtcagaaa aattaaaact gaatactcgt
     2221 gtcactttaa ttcaccaaga tattctacag tttcaattcc ctaacaaaca gaggtataaa
     2281 attgttggga atattcctta ccatttaagc acacaaatta ttaaaaaagt ggtttttgaa
     2341 agccgtgcgt ctgacatcta tctgattgtt gaagaaggat tctacaagcg taccttggat
     2401 attcaccgaa cactagggtt gctcttgcac actcaagtct cgattcagca attgcttaag
     2461 ctgccagcgg aatgctttca tcctaaacca aaagtaaaca gtgtcttaat aaaacttacc
     2521 cgccatacca cagatgttcc agataaatat tggaagctat atacgtactt tgtttcaaaa
     2581 tgggtcaatc gagaatatcg tcaactgttt actaaaaatc agtttcatca agcaatgaaa
     2641 cacgccaaag taaacaattt aagtaccgtt acttatgagc aagtattgtc tatttttaat
     2701 agttatctat tatttaacgg gaggaaataa ttctatgagt cgcttttgta aatttggaaa
     2761 gttacacgtt actaaaggga atgtagataa attattaggt atagggcacc tctaataact
     2821 accaattaat tcacctcaaa atgaaattag aaaaaaacag cgaaattttt aaatctattt
     2881 cttatcgata caaattcctc gtaggcgctc gggaccccaa catgaggaaa tgtttttata
     2941 aaaaaagagt agttaccgtt ttttggtaac tactcttttc cttttttaag gtttaaatac
     3001 ctggaagttt aggtgttttg ggtattggtg gtgtaggtgg ctgcaagctt tccaattttt
     3061 gttgaattgc tttaaattca ggtgtctctt ggtgtttatt tagaaccatt ttttgctcta
     3121 ttcgttctaa agtcggttga atttctttcc ctgatacttt acgattcaat tctttttcta
     3181 aggtttcttt tggactaata gaaggtgttt ccaaacttga atagagtttt actccttctc
     3241 tgttatatag ccttatatcg ctaaataagc ccgttttatg aagggtttct aaattggtcg
     3301 gtaagttttt gacaacaata tcatgcgctt gttttggtgt tgccctggct gtcattggat
     3361 catctgcata catggtttca tatcgttcaa ttgttcctaa atatgagttg attttaggta
     3421 ctgccatgac atacattttt gtttcataac ctttggcttg aagcattgtt gcggtttgaa
     3481 taggaacgtc tgttgttcgt cctgtacctt cgatcaccaa attataccct tgatcgctca
     3541 aacggcttat gatcgcttct gtcatgcgat tagaataagg ggtaacgtgt tttactacgt
     3601 ctttttcata aagtttcact agttcatcaa aattagggtg ctgttgttta aaggtatcat
     3661 tatcaatgac aataacattc ccttgtgttt cttcaaaaat tgctgatcgc aaactggttt
     3721 tccctgaccc tggttgccca ccaagtaaaa aagcggttgg cgattcaacc gctttttttc
     3781 cttgaatcaa ttcttctaaa ttatcattta agcgattctc aaattgtttg tcagtaaaat
     3841 tgactatatt tgccatatta agccactttc tctttattca aaacttgttt tgagtaacga
     3901 ttcattgacc gccaatactc atgaacggct tgtaattctt ctaaagaata atcaaaaaga
     3961 tttacacgtt ttgcaagctt taattggttt aataaattga cttccaataa ttgagagtca
     4021 tttttattta attcatggtt caaaacatag tttaatactc gattataaac gcttgccgat
     4081 aattcgttaa tgatctctat ttcaaatgtt ttttcatacg taactgccat ttcattttca
     4141 ctccttttaa agtttgtcag gtaaatattt ccgtatatat tcttctaagg cttgatccat
     4201 aacttctttt acgtttccgc cattctttgc tgtttcgatt tttatgatat ggtgcaagtc
     4261 agcacgaaca cgaaccgtct tatctcccat tatatctttt tttgcactga ttggtgtatc
     4321 atttcgtttt gctttttgtg cgcctaaatt tcccacaatc actcacttct ttctatttct
     4381 tcttactctt attttatcat caacaatcac aaatcacaag tgatttgtga ttaatcactt
     4441 gtgatttgtg attctattct ttttcgtttt ctaattgaat aattcgctca agcatttcaa
     4501 aaaatacgtt cttatacatg gataaaactt ttttgtcata gcctttatgc tctgtaatgc
     4561 cgtttttaga ccaggtactt actttattac ttcgcttgat aatattttgg aaaaccaagt
     4621 tatcttcttt atgttgctta tacagttctt ccaggtttga ttttatcgtt gcgctgtccg
     4681 tatcaactaa ataaggaaca aaaccgatc
//
LOCUS       DAJSBL010000113         4647 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.116, whole genome shotgun sequence.
ACCESSION   DAJSBL010000113 DAJSBL010000000
VERSION     DAJSBL010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4647
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.116"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(133..717)
                     /locus_tag="ONT85_002774"
     CDS             complement(133..717)
                     /locus_tag="ONT85_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR2379695.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
     gene            complement(928..1131)
                     /locus_tag="ONT85_002775"
     CDS             complement(928..1131)
                     /locus_tag="ONT85_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2379696.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            complement(1141..1563)
                     /locus_tag="ONT85_002776"
     CDS             complement(1141..1563)
                     /locus_tag="ONT85_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2379697.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRNGIAIGNPTNDLTVTENYLIVSPLLARAASKTIRVTGTMIFDKVGWPSRPPQSR
                     NWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            1801..2415
                     /locus_tag="ONT85_002777"
     CDS             1801..2415
                     /locus_tag="ONT85_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR2379698.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDYVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVKLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSADAKDPQ
                     RRLVYKSIVEDLKNGVAIAKIAKDYNVTRQTVYRIKKDSMVNDK"
     gene            complement(2580..3404)
                     /locus_tag="ONT85_002778"
     CDS             complement(2580..3404)
                     /locus_tag="ONT85_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2379699.1"
                     /translation="MIERLKDFMISVWDVAMLADESNYTSSIYRLEDSNWIEISLELK
                     TLPNSVNYIIRDITSEAQQLVNYMHGHESIPFSLILLKRSKNISNTRDRFISIITACE
                     IGVKEFYQQEKPDLTLIFENLQSPPVDKLLGSMFKGYFNIEFPKEIRKEIKNFIEKRN
                     KLVHSPQGSKPTIEECFECYMAVLKTFNFFYKINDSFMYRDFYEESIELTPTEPNKSQ
                     ITLSKELERKVESQELAIGISITNVSTYKELYEAKSQDNKDFVENIDKEPTNTVGD"
     gene            complement(3428..3583)
                     /locus_tag="ONT85_002779"
     CDS             complement(3428..3583)
                     /locus_tag="ONT85_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2379700.1"
                     /translation="MEYKFNLNLKEVLPTQGTININGQDNDDDIVIKKIEFIDSDINV
                     FFILKKN"
     gene            complement(4042..4299)
                     /locus_tag="ONT85_002780"
     CDS             complement(4042..4299)
                     /locus_tag="ONT85_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HCR2379701.1"
                     /translation="MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLG
                     KPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYSAKDHY"
     gene            complement(4292..4561)
                     /locus_tag="ONT85_002781"
     CDS             complement(4292..4561)
                     /locus_tag="ONT85_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HCR2379702.1"
                     /translation="MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKR
                     DYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTHDLIEVESDD"
BASE COUNT         1533 a          815 c          627 g         1672 t
ORIGIN      
        1 taatattaac aaatgaataa aatccttaca acatttgaga ataagttatc agtagacact
       61 tacaaaaaat tctcatacta ttgtcacttt tctagataaa tggcaaaaat tttcagcatt
      121 aaatgacaaa aactaatgag aatttgtctt tccatttttc tcctccattt ttgccaaata
      181 acgataaatg gtggttcttg agacgttcca ccgttccgca atagtcttaa taggtgtacc
      241 gctatccact aaagtcttca gtaattccaa atcttgttca gacaattttt caggacgacc
      301 accaaaccgt cctcttgcac gagcagctgc tcgtcctgct gcggatcgtt ccaggatcaa
      361 attacgctca aattctgcaa atgccgcaaa caaatgaaac atgagctgtc ctgtagaact
      421 tgatttgtcc atcgtaatat tttcttgaat actatgaaaa ctcacaccac gctcattcag
      481 tcgattgacg attgaaatca aatcttccat atttctacct aaacgatcta agcgccaaac
      541 gacaagtgta tcgccagagc gaacaaaatc aatggccatt tctaatcctg gtcgattact
      601 ttttgcccca ctcatatgat cagtaaaaat tttttcacaa ccaaatttat ttaggctgtc
      661 ttcttgtaaa tccaaatttt gtaaaccagt agatacccgg gcgtatccta ttttcaaaaa
      721 aatcactcct tcattaattt tattgtaaca taactttatc catatagttt aactgaacat
      781 agatattgtt atgggtttat gaacatgaaa tgaattgttt ttttgaaaaa taaagcaaaa
      841 aaaggaagtg tccagaaatg ggtgatttta tgaacacttt caattagaaa aaaagaatgt
      901 cgactagaca ctcttttttt aaaatcatta tttttctttt tgatatgttt tttggtctct
      961 tttttttctt ttttcttcag aatcttctag attagaaatt gatttttgaa ttttttgtgt
     1021 ctcctgtaaa gttcgctgaa cttgtaaagc tgattcttct ttcggtaaat taattttact
     1081 tagaatatcg ctatcttgat aaaaatttgg tgtgttcaca aattctggca taaaaaatcc
     1141 ctactttcta gttaaggttc ctgaataagt cgcaatccaa ttgtttccag ctgatttata
     1201 actttgtaaa tacaattttc catagtacca ataaccattt cttttttctt tccaattccg
     1261 tgattgagga ggtctagaag gccaaccaac tttatcaaaa atcatcgttc ctgtcactcg
     1321 tatcgtttta gaagctgctc gagcaagtaa tggagaaact attaaataat tttcagtaac
     1381 agttaagtcg ttagttgggt taccaatggc tattccgttc ctatctaaag gagttaattc
     1441 ttggttatta tctactacag tagttggttc atttgtacta tcagcaaatg taggaattgt
     1501 agttccagtt acactaattc caatagttaa aatacctaaa gataaaataa acttattttt
     1561 cattgtctat cacctcgaaa tcaaaaataa gaaaacgttt tcttaataaa tcttaacaga
     1621 taaatcaagt tatatactag aaaattcata aaatatattt ttaatgtcat aatcgtttaa
     1681 attttcttga ttgacattta tttacaaaaa atattgccat ttaaaaagaa aagtgacata
     1741 tggcttcctc gtgtatgatt agggtgtatt ttaaatagtt gcagaaagta ggtaaaagtt
     1801 ttgagagttg catacatacg tgtctcatcg atcgatcaaa atgaacaacg ccagattgaa
     1861 gaaatgaaaa aattcggtgc agagagaatt ttcatcgaga aacaatctgg cgcaacgatt
     1921 actcatcgac ctgttttcca agaagcatta gattacgtcc gagatcaaga tatttttatt
     1981 gtggaagcta ttgatcgact tggaagaaat tatgacgaga tcatcgcttc agtcaattac
     2041 ttgaagaaga aaaatgtgaa gctgatcatt actagtttac cgatcatggc tgaagcaatt
     2101 ggcaatcctc tcctggataa attcattaag gatctgatca tccaaatttt ggccatgatc
     2161 gctgaacaag aacggactga atcgaaacgt cgccaggcac aaggaattaa gatcgcaaaa
     2221 gcaaacggcg tctacaaagg gcggcccaaa ttatacagtg cagacgcaaa agatccccaa
     2281 cgtcgccttg tttacaaaag catcgttgaa gatcttaaaa atggtgttgc gattgctaag
     2341 attgctaaag actacaatgt tacgagacaa accgtgtata gaataaaaaa agacagcatg
     2401 gtcaatgaca aatagttgat catgctgctt ttattatctt ctcatcttta atttcctaca
     2461 tataattact atctataaaa ttaagtctta ctcagaacag ataaagcata aaattccaac
     2521 aaaccatttt ttacttaact aaataaagag tctgttaata taatcttcac ttatttacgt
     2581 taatccccta ctgtatttgt tggttcctta tctatatttt ctacaaaatc cttattatct
     2641 tgtgatttag cttcgtataa ttctttgtat gtcgacacat tggttatact aattcctata
     2701 gctagttctt gagactctac ttttctctct agctcctttg acaaagtgat ttgagattta
     2761 tttggttccg ttggagtcaa ttctatagat tcttcataaa aatctctata cataaagcta
     2821 tcattaattt tataaaaaaa attaaatgtt tttagcactg ccatataaca ttcaaaacat
     2881 tcttctatag ttggtttact tccttgagga gaatgaacca atttattacg tttttctatg
     2941 aaatttttaa tttcctttct tatttctttc ggaaactcaa tattaaagta ccctttaaac
     3001 atgctcccta ataatttgtc tacaggggga gattgtaaat tctcaaatat tagagttaaa
     3061 tcaggtttct cttgttgata aaattccttt acccctattt cacaggctgt aattatcgaa
     3121 ataaacctat ctctagtatt tgatatattt ttacttcttt taaggagtat aagagaaaag
     3181 gggatacttt cgtgtccatg catgtaatta actaattgtt gagcttcaga agttatatct
     3241 ctaattatat agttaacgga attaggaagt gtctttaact ctaatgaaat ttctatccaa
     3301 ttactatctt ctaatctgta tatgctcgaa gtataatttg attcgtcagc aagcatcgct
     3361 acatcccaaa cactgatcat aaaatctttt aaacgttcaa tcatttcagc aatattgcta
     3421 aaatcagcta atttttcttt agaataaaaa aaacatttat atcagaatca ataaattcta
     3481 ttttttttat tactatatcg tcatcattat cttgaccatt tatgtttatt gttccctgag
     3541 tggggagtac ttctttcaaa ttcaagttaa atttatattc cattacttct cctccaatat
     3601 ataaaaatta cttaactgta tccaaattta atatgtaaat aaatcattaa tttttagcta
     3661 aattctatga gaagtttatt tttgttgttt ttgctaatta actaaaacta gtacaaaaaa
     3721 tttaaaatag attattaaac ctattgctta ttctattcag tcatatatat aagcgtcatc
     3781 ctttagtaac taacatccca cactacaata gtattataac atatctattc aacttcaaat
     3841 tgttgaaatt taagccattt atatttactt ttcctcaact ttagaaatga ttttactaac
     3901 cttatatccg taatacgcgt aacaaacaca ttcattaata acaatcaaca ctaagctata
     3961 ttagcttttt aaatgactag ggtaaataaa ataaggatca aaaactttta agtttctgac
     4021 cctttcctta cttccgattg gttaatagtg atcttttgca gaataaataa atatcgtttc
     4081 attttcaact ctatatatca gtctatgttc atctgtaatt cttctggacc attttccaga
     4141 taaatcatgc tttaatggct caggttttcc taatccagca aagggggaac gatcgatatc
     4201 ttttattaac ttgttaatct tttttatatt gcttttgttt ccttgctcat gccaataaag
     4261 ataatcatcc caagcatcat cagaccaagc cttaatcatc agattcaacc tcgattaagt
     4321 catgtgtttt aaatgcacct ttggagaatt gttcatctcc tcgacgaatt ttttccatga
     4381 cgtaattatt agaaagtgtt ctcaacgttt cttgcataga atcataatct ctttttgata
     4441 atacaacaac tgtatcttct acatctttac ttgttacaat aagtgtttca gcatcctcat
     4501 taacttgttt catataacta cgtaaatttt ggcggaaatt tgaataagct actgcttcca
     4561 ttcctttcac cccttattta tttctataaa acaattgtac attatattgt acaattaagc
     4621 aaatgattaa tattctaact tcttata
//
LOCUS       DAJSBL010000114         4608 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.117, whole genome shotgun sequence.
ACCESSION   DAJSBL010000114 DAJSBL010000000
VERSION     DAJSBL010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4608
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.117"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(83..337)
                     /locus_tag="ONT85_002782"
     CDS             complement(83..337)
                     /locus_tag="ONT85_002782"
                     /inference="COORDINATES: protein motif:HMM:NF024673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCR2379703.1"
                     /translation="MTATEIRNNKLKKKISTIFFTNKQRYGATKIHQVLLKEGISVSL
                     KHVQKLMKQLNLRSIVVKKYRPQRSNKPIMDRLQLTRQKN"
     gene            complement(448..702)
                     /locus_tag="ONT85_002783"
     CDS             complement(448..702)
                     /locus_tag="ONT85_002783"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR2379704.1"
                     /translation="MTRYEENFKQMIVELNQTGRSVRGLAKEYGLSEATIYKWKNLYL
                     PDQSTGLTGKEVAELRKENARLNEELEILKKAAAIFSRKT"
     gene            complement(895..1170)
                     /locus_tag="ONT85_002784"
     CDS             complement(895..1170)
                     /locus_tag="ONT85_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgO"
                     /protein_id="HCR2379705.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
     gene            complement(1142..2095)
                     /locus_tag="ONT85_002785"
     CDS             complement(1142..2095)
                     /locus_tag="ONT85_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HCR2379706.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            2707..4200
                     /locus_tag="ONT85_002786"
     CDS             2707..4200
                     /locus_tag="ONT85_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HCR2379707.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            4335..>4608
                     /locus_tag="ONT85_002787"
     CDS             4335..>4608
                     /locus_tag="ONT85_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HCR2379708.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELR"
BASE COUNT         1492 a          774 c          801 g         1541 t
ORIGIN      
        1 cttgaattct tgtgaaaaag ttcttcgtgt tctcttagac ataaaaattc ctcctggtat
       61 gttttcttct agtctacaca ccttaatttt tctgtctagt taattgtagc ctatccatga
      121 tcggtttatt agacctttga ggtctatatt ttttaactac aatcgacctt aaatttaatt
      181 gtttcattag cttttggaca tgtttaagag atactgaaat cccttctttt aataaaactt
      241 gatggatctt tgtggcacca tagcgttgtt tattcgtaaa aaaaattgtt gaaatcttct
      301 ttttcaactt attatttctg atttctgtag ctgtcaacgg tttattctta taaaaataat
      361 agacacttct agggatttct aataaccgac acaacaaaga aacagtgtaa tccttacacc
      421 atttttcaat aaattgaaca agcgaattta ggttttccga gagaatatgg ctgcggcttt
      481 ttttaagatt tcaagttcct catttaaacg agcattttct tttctcagtt cagctacttc
      541 ttttccagtc agtcctgtgg actgatcagg taaatataaa ttcttccatt tgtaaatcgt
      601 tgcttcagat aagccatatt ctttcgctaa ccctcgaaca gaacgtccag tttgattcag
      661 ttcaacaatc atttgtttaa aattttcttc atatcgggtc ataaaaattc atcctctcgt
      721 agataagttt aatttattct tgtctctacg aaaagctgtc taagatttga tactaacacc
      781 aaatgttgta tacccccaaa caatacacat attttgaagg attaggatta ataaagctaa
      841 aaatatagta agtatactaa atatatttac tatatctagt atacttacta tattttaatc
      901 ttttgatcgt ctattatata taatccgttc ctcgtcgttt aaagtatcaa cataggcgtc
      961 cattgcaagt ttgactattt catacatagg aatatctttt gcataactaa gatcccgaat
     1021 ggtttttagt acgggaggat ctacttgaac tggttttctt tctttcgcag tgtactttcg
     1081 ttcccctaac tcgacattaa tatcatcttt aattgaaata tcttcagcac caattttctt
     1141 cttatctttg ttcgttggta ataatgccat ttagtttaat ctcctttcca agaggtagga
     1201 tatgattggc cattgagtca gcatatttag gtgtataatc aaatccttca atatcaccat
     1261 gttgttcata gtattcaatt ctttcttcta attctgtaaa gatgtctgca aaaatagccc
     1321 accatcttcg atctacataa tcatttaatt ggataccatt ctcaccatac atttctaacc
     1381 gatccgagcc tttaacaatg cttttaaaaa tgtttgattc accatactta tcaattgttt
     1441 tttgatagtt tgcgtgttga gaggaacgtc gcactgagaa aagaacaggc aaaattccaa
     1501 tgatttgaag atgagactct tcaaaattcg tgacaatcgg aactaaaact ttattaataa
     1561 aatcttctgt tccttccatt gcgtaccgtt ttaattcttg cattgcaata atataatctg
     1621 cagcagttaa gaatgcacga accacattat ctgttgatgg tgggcagtcg ataaagatat
     1681 aatcgaaatt ttttctcaat tcttctaaag gtttaatgaa ggccaatgat ctcttacgac
     1741 gatcaggata tttttcggac aagtattcat taagttcttc agatccagga gagccagcca
     1801 taatatataa atgtggactt acttgagtaa tacctgaaac ccagtcccct tttttgattg
     1861 ctgcagacat ggatcgatca aagctagtaa tttgatacgt tgatccaatg ttggaagtag
     1921 tatttctttg ttggtcataa tcaattagca acacgtcttt ttcaaaaagt tgactagaaa
     1981 caatcgctaa taaatcattg tctgtagtct taccaatgcc gccttttcta cctgtaagca
     2041 ttacaacttt tcctagttta tctgatgttt taaatgcagt ccgtaagttt tccatagttc
     2101 cctccaattt cctatactaa atatatttag tatacttcat atacttaata tattcgatat
     2161 atctaatata ctccaaattg ataagaattc agactttata aggaatatag tggatatact
     2221 aaatatattt gctatattca gtatatcaga tatattcaaa atgtcaaaat atatttgtgc
     2281 gttttgcgtt gacaatttag agtgaaaaga ggtaaactta attctataga aaccacagat
     2341 aattcactat ttacaaagta gcaaaagcag tggtactctt taaacagaaa aattacataa
     2401 aaaaaagaga cggaaataat tgattttagc agaatcaatt atttctgaat cacatggtta
     2461 ggacatgtgt tcacgctcgt ctcaagaata tgtaagtagc taccaactac ttatattaaa
     2521 agttgatcgg ataccttttc cgattgcctt tattctacca aaaaaaggcg agcaactcaa
     2581 cagaagaacg tttatttagt gtacttaaat taaatattct taaacactga agagagaaca
     2641 catactatgg ctgagattaa agctaaactg tgttctcttt ttgtttgaga aaggacgtta
     2701 caatcgatgt tgagcgaaat ttattcagct attcttaaaa acggcttacg tacatacaaa
     2761 tatcgaaaca gcaccatgaa acctgcaggt catattgatt ccagtaggag tggtgctatc
     2821 tttgcgtacc gtagcaagca attaatgaat gttgggcgtg gtatggtcat tacttcggaa
     2881 gaagctattt tagagaacga aaagaaacta acgcattgga cacctaatac ctatcgcttt
     2941 ggtacttatg cggatgatta tcgaacagtg gttaaaggtc attctgagaa aaacttaagt
     3001 caaatcaaca catttgtagt tgatatcgat tcaaaagaga accatcaagg cgaaattatt
     3061 ttagcctgtt tagatcaagt gggctacatg ccaacgctaa ttttagaatc agatcatggt
     3121 tatcaagttt attttgtact aaagacacca ggctatgtca caaagaaaag caatttcaag
     3181 gtgatcgatg tagcaaaaaa aatttcaaaa acaattcgtc tacagttggc tgaaaaattg
     3241 tccggagtgg atctaggatg taaccacttc ggaattgctc gattcccaca agaaaataat
     3301 attgttttta aagaaatgaa ctatcagtat tcttttgctg actggattca atggtcgatg
     3361 aagcttgaat cagatcaaaa aacaaaagaa gatcgagaac gaaaactaat tgtctttcct
     3421 gagaaaaaag aaattcgtca agtagatgaa ccgtggttcg atatgttact tcataaagca
     3481 aacatcgtcg gcggagaagg acgtcttggt cgaaataatg tgattttcac gctttcttta
     3541 gcttattact cttcaggtta tgcattggat acttgcgaat acaatatgtt cacattcaac
     3601 gaacggttac aggaaccgct atctgaagca gaattagtaa agattgtacg tagtgcttat
     3661 tctggtaatt atcaagcagc aagcagagaa ttcattattg aattgtgcca gcaatgggtt
     3721 tcaccaacct taaaaaataa tgatctgttc attcaacgac gtggctggtg gaaatttaaa
     3781 aagccacgag atcaaagaaa atatagtcat aaaaatgaat ggaaagaaga tctattggct
     3841 tatctaagcg aaaaaagtct tgctagtgag ccttatttga acctgtcaaa aaaagaattg
     3901 gcttcacaac taacgatgcc caagcgaaca cttgataaga tcttaaatga gttacgagag
     3961 gagaataaag tcttctacaa ggtaacacga ggaagaaatg gcggaatgat cattgcttca
     4021 gtcaaaacgt tgttcgcgag ggttattcaa ctaagtaaag aaaagagact agattattca
     4081 acaaggatca cagaggtatt tggactcaac gagagcttag tagaagagac atttagacaa
     4141 ttatcaaccc ccttgaaccc tcttaaagaa gtgaatcttt ttgaagtaga tacaggttga
     4201 aaaataaaag gtgcaaattg ccttatacaa tatatctatg atacttctgt aggtgttgct
     4261 ccctttgcct agctacgtgt aggtggtata cttataactg cgtatgcagt cttatgtatc
     4321 atggaggaag ttgtatgtcg ttaaaaaatt atgtctattc acatcctgtt aatgtgttta
     4381 tcattggtcg tttgggatta tctgtggaag tgttctgtga gctttatggt tttaagcagg
     4441 ggactttgtc ttcttgggtt accagggaaa aaacagttgc ttctttgccg attgagtttc
     4501 ttcatgggtt gtccttagct tctggggaga ctatggatca ggtatatgat tgtttgtgtg
     4561 tgttagagca ggagtatatt gagtataaaa tagctaacga gttaagaa
//
LOCUS       DAJSBL010000115         4470 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.118, whole genome shotgun sequence.
ACCESSION   DAJSBL010000115 DAJSBL010000000
VERSION     DAJSBL010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4470
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.118"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            318..1049
                     /locus_tag="ONT85_002788"
     CDS             318..1049
                     /locus_tag="ONT85_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HCR2379709.1"
                     /translation="MMKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEA
                     GDEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYA
                     AADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAV
                     SLVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSV
                     PGKSVKLAQQYSTKLVWTLEDTPNN"
     gene            1194..3221
                     /locus_tag="ONT85_002789"
     CDS             1194..3221
                     /locus_tag="ONT85_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HCR2379710.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGHSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRIDIPVENQRIGTFEGKLSWILADVPGN"
     gene            3329..4426
                     /locus_tag="ONT85_002790"
     CDS             3329..4426
                     /locus_tag="ONT85_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HCR2379711.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCILLFVLRKKEEKEK"
BASE COUNT         1569 a          717 c          923 g         1261 t
ORIGIN      
        1 atataatata cattttttat ttaattataa ttaatattga ttgaagtcta ggcagtcaag
       61 tcacattttg atagatagga gggggactta cgaattattt taaaggctat gacagctgta
      121 tttctagatt gtgtttgcat caagttgcaa taaaaactag aaatcaaatc gggctgataa
      181 tcatttataa ctaatcgata cataggacca aatataggtc tatgttcttt tatttcataa
      241 gtatcgtaaa acagaatttt gctatggatt aatccaatcg actgatatgt ttagtaaata
      301 aagaaagagg aatgtcaatg atgaaaaaag aaatcgtatt aacagcagca atgatgttat
      361 tcagcatggt agcaagtacg acttttgttt ctgcaactga agtataccca aaggaatata
      421 acacagaagg aacgattaca tttgaagcag gagatgaggg tgtaactcct cctgttgatc
      481 cagagaatcc tgatcccaat aaacctgtcg atccatcaga tcctccaagc cctggaacag
      541 gaggggcatt atcgattgat tatggatcaa aattcaaatt tgggactcaa aaaatttcta
      601 ctgcggataa aacgtattat gcagctgctg atgtgatgaa cgatgggtct agaaaaccga
      661 catatgtcca agtgacagat aggagaagta ccttatctgg gtggaaatta agtgtctctc
      721 aaccagaaca gtttaaaact gccagtgggg atgaactagt tggagcacag ctgaaattta
      781 cgaaagggca ggctgtttct ttggttgatc caacatatac gccgcaaacg gtgaattctg
      841 aattaacgtt gacaccggga ggaaacaata cgttagcaat taatgctaaa tcaggaacag
      901 gtgtggggac ttgggtctat cgatttggtg caaatgaaaa tgaaaatcaa gatgcggtac
      961 agctttcggt acccggtaaa tctgtcaagt tagcgcaaca atattccact aaattagttt
     1021 ggacattaga agatacacct aacaactagt ttactgaaaa gaactgttaa aaataaatac
     1081 gtagtcatat tgtcttgcga ggtaatcgag caaaaattgt caaaggctag gctaaatttg
     1141 tttggatacg aatagttcat ctaggaattt aacgatcagg aggaaaagat atattgaaaa
     1201 agaggaggat cgtgctcatt ttaagcagtc tcttagtgac taatatcttt ccgatcatag
     1261 ggtactcttt ggaattgaat cagttggagc atggaaatag aagagacgaa gaaagcaaac
     1321 tcacttcgac attcgacagt ttaatagaat ccaaccagac acaagaaagc agtgaggatt
     1381 taagtatttc gagtagtgga gagaccactg acaacgtaga agctgagggt tcggacaaac
     1441 aaggactaga agatgcacca atttctgcgg ttgagctgcc tgacgatccc aatattattt
     1501 ctatcgataa aatttttcaa acacctatcg gtactagcac gtccatattg gaaaatggga
     1561 agttactgca gttgaaccca gcagaaaggt ctcaaagagg agctatttgg tcaaataaga
     1621 agattagttt actctcggat tttacattta aatcttattt atacttagga aatcaacgag
     1681 atagagcggg agatggcatg acctttacat tgactggcga tcctagaatg gctagtaatc
     1741 ccagtcaagt tattggatcg ccaggtatgg gaattggctc atattctact cgttctggcc
     1801 aaccttacat tgcgaatgcg ctctcgattg agtttgatac gtataaaaat agtggttctt
     1861 ctgatcgaat ggatcgagag ataactgctg ataatagagg gcatggacat gtcgcttttg
     1921 tcacgcctaa accaaataat aataattatt ctggagagca tactggcgtg acggtagctc
     1981 ctacatttct ttctaatggc acatggcgct tgttaataat taaatgggac gcggagcaaa
     2041 gaagtcttac ttatgatttg gaaggtgtcg gtcactcttc tcggattata cctgatctca
     2101 ttaggcagtt tggttctaca gaagtaaatt gggggtttac ttcttcaaca ggtacttttt
     2161 ttcaagaaaa tgctgtcgct gtcacgcgac ttccatcgaa tgtcacatca acagctgatg
     2221 taaaagtcaa cgatagtgat tatggggcca tcgctgaagc tggaggaaac gataagataa
     2281 ctatcagaaa tacattgaag gtcgaggggg attttcttga atataagaaa atgcagacta
     2341 gtatcgacct cccacaagaa ttaactattc ccgagcatat cttgatcaat ggaaatcaaa
     2401 ttgatgccaa agatatcttg ataaccggaa atcgagtcac gctaaatctg acagattatt
     2461 ttattgctgg agatacgtta ataatcgaat tggagacaac tttgcaggaa gtaattcctg
     2521 aaaaagtact cactatgcat tttgaatacc ttgaaaacgg gaatttgatt gagcaatcga
     2581 atgaaataaa cctaaagatc gcaaagctga aggagataga tatccatgtt tattataaag
     2641 ataaagacag tcagattgat attgctgaaa tgaaaacaat cactgggaaa ataggagaga
     2701 agtataaaga aactcctatt gatattgaag ggtatgtttt tgactcggat tcagcgaata
     2761 cagaaggcat cttctccgaa aatacagagg atatctattt ttattacaga aaaggcaaac
     2821 tttatctctc aactgctcct caatatgtca attttggaaa acataaaata tccagtagac
     2881 cattaagtga attcggacag ttttcaggtg ggttgaaaat tatagatgaa cgggcttttg
     2941 gaagttggaa attacaattg aaacaagaga aagttcttac aaatggagat tttgagcttc
     3001 cagaagctct ttcctttgtt acctctgaaa atacaacggt tatagggaca tccgctgtta
     3061 cgattttcga aagtgatcaa aagggtgaaa gcgatttgtc ctctctttta gattctacag
     3121 ctcatcgtgg aatcagaata gatattcctg tagaaaacca aagaatcgga acctttgagg
     3181 ggaaactatc ttggatactt gcagatgtcc caggaaatta acaggaagag aagaatataa
     3241 caaaattatt gagtttcaag aagggcacag gtaccatagg ctcttgtgtc ccacagagac
     3301 aagattttgt tatagtcttc tcttacatat gattaaagga gcgattttct tgagaccaaa
     3361 acaacaatta tttataacac tcctagttat tttaggggca ctttttaaca atatatcagt
     3421 gtcagctagt gcatttaatt tttcagtgac gccgataact tcagagaatc aaatcgacaa
     3481 gagaaaaact tacttcgatc ttcaacttga tccggatcag gaagtagaag taaaagcgga
     3541 attgagaaat gatacagaaa aagaagtgaa aatcgatatt tctgtgaata gcgcaaccac
     3601 caattcaaat gtgatggttg aatacggaaa aaacgagatt gaaaaagaca tgagcctgat
     3661 ctttgatctg gtagattatg tgagctatcc acagatagtg acgctaaaac ctaaaagtgt
     3721 ccaaaccgtt gcttttcatg tcaggatgcc gaatgagcga tttgatggtg tattggctgg
     3781 tgggatcaca ttccaagaaa tccagacgga aaaggaccaa acagaaacta aggatcaaag
     3841 cttatcgatt gagaatgaat acacctatat tattgctttg ttgatgcaac agacactgaa
     3901 cgaagttgct ccgaacctta ctcttcatga agttaaacca gatcaaatca atgcaagaaa
     3961 tgtcatctta gcaaacgttc aaaatgatca aaaaacctat atcaatcaag tagttattga
     4021 aacaaaaatt acaaaaaaag gacattctga agttttatat caagaggaaa aagaaggatt
     4081 gcagattgcg ccgaatacaa atttttcttt tccaactgct ttaaatggtc aaccgctaac
     4141 cccaggtgaa tatcacctta caatgactgt tttaggaaat gaaaatgaaa atggaaaatt
     4201 ttcgagaaaa aaaggaaata ctacgatcaa ctataccaat caatgggtat ttgaaaaaga
     4261 ttttacaata gacggaaaag tagccaagga attgaacacg acggatgtta cgattaaaaa
     4321 agacaataat tggatttata tcttagtagc ttcacttttc ttattgttta ttttattatg
     4381 catacttttg tttgttttga gaaaaaaaga agagaaagaa aagtaaggtt aagataacaa
     4441 atagtaaatt tttattattg taggatggtt
//
LOCUS       DAJSBL010000116         4458 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.119, whole genome shotgun sequence.
ACCESSION   DAJSBL010000116 DAJSBL010000000
VERSION     DAJSBL010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4458
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.119"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..115)
                     /locus_tag="ONT85_002791"
     CDS             complement(<1..115)
                     /locus_tag="ONT85_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017370594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HCR2379712.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDR"
     gene            246..698
                     /locus_tag="ONT85_002792"
     CDS             246..698
                     /locus_tag="ONT85_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2379713.1"
                     /translation="MKKTILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            712..1401
                     /locus_tag="ONT85_002793"
     CDS             712..1401
                     /locus_tag="ONT85_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2379714.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            1526..3877
                     /locus_tag="ONT85_002794"
     CDS             1526..3877
                     /locus_tag="ONT85_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1906 domain-containing protein"
                     /protein_id="HCR2379715.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
     gene            3957..4352
                     /locus_tag="ONT85_002795"
     CDS             3957..4352
                     /locus_tag="ONT85_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2379716.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
BASE COUNT         1567 a          573 c          885 g         1433 t
ORIGIN      
        1 cacgatcata taagatttct tgaacttcac gatagctaag gttataacga agatagtagc
       61 ccacggctac aataatcaca tcctgctgaa attgctttcc tttaaaatga ttcatcgtta
      121 ttcctcctgc tatctttttc tattattcta tcttatttga tagtagattt aaaactttgc
      181 aacagaacca attatcttat cgtagatata tattataaat ttaataagaa agcgaggtga
      241 gaatcgtgaa aaaaacgata ctaagtattt tttcagtatt tacaatatta attgggggct
      301 attttctttt gaatttagat tctgttaatt caaacgctca aataaaagat caaagagtag
      361 ctgaacataa taataaaaaa atgagtttga ccaaaaatat ctattctatg tcagatgaag
      421 aattgtataa agaatttggt gatttatccg gggaacagtt agaagaaagt attccaggaa
      481 ttagtgactc agataaaatt gttgtcttgt ctgctggtgg gcaattaatg ggagatggca
      541 aatccgaatt agttagttat aaacaaaatc acagacagtt tattgatgtt aatactgatt
      601 ccaacgctat ttcgattaag gatctaaaag agcttttaaa tagtaaaaga aatgaattaa
      661 agcagataaa agaaactaat tcagtggaat tagagtaaag gaagattcat tatgaaaaag
      721 atttttggtg tttctgttgg gctacttatg atgttaacat ttggaaatgc agtatatgct
      781 gctgagaaag aagttatttc agatccagag cagtacgcct cacgatactt tgctgatttt
      841 gaagaagaag ataaagtact tgcattgcca gaaggtggtt ttttaaaagg ggaagctaaa
      901 attgtatcta tggataatcc agatataatt caaggttcat ataattcaga aactgatcct
      961 gcttcagttt cagtgacaga agcaaaaaaa attgttgctg aaaaattcaa aaatgatatt
     1021 gaaactactc caattatgcc tcgaggagca tttccacccg atgttggtac tagcggacac
     1081 cttcttgtac tagcaggagg aggaagctat aaatcacagc catttagtgg tgcgggatgg
     1141 cgttttggag gtatgggtgt aaaaccagct gatggtacag gcaacttttt acgttggaaa
     1201 tcctacattg acgatggaag agtaggaaat gtaaatcaag ccaacgcaac taaatctggt
     1261 tctattcaag gaatggcatt gtatgctcgt gaaggattca aatactttgc accaaacggg
     1321 cctggttgga caatgaatat aggtatttat tacacttata atcctgttgc aggtacgtat
     1381 tatgttgttc aaaatattta ataaaacagc acaaagagat ttctttgtgc tgttttatta
     1441 tttttagttg ttgacaaaca ctaaaatata aaatataatc aacacataaa cgaacgtatg
     1501 ttcggcaaga aagcagggga tttttatgga tgagatggtt ttaagtactc agaaatggct
     1561 aaacaagaaa tacagtaatg ttacagggtt tgataaggtt ccagaaaatg ggcgaacagg
     1621 gtggcctaca atctatggac ttattgaagg actacaagta gaacttggta ttactaatct
     1681 agtagctaat tttggaccta ccactgaaaa aatgtacgat aatcaggtta ctcctaaatg
     1741 gggcaaaaat ctacctaaaa atattgtatt tcttattcaa ggtgcctttt ggtgtaaggg
     1801 gattaatcca ggtggcttcg atggggtata tactccatat ttagatactg ctgtaaaaga
     1861 gttgcagact gatgcagggt ttaatggaag cgctgtaact ggagttttgg atgcgaaatg
     1921 ggccaaagcg ttatttgata tgagtgcttt tgttcttgtt cccggcggag atagtaaaat
     1981 tagaaaaatg cagcaatggt taaatgtaaa ttatttagaa tacacgggaa taatgccttg
     2041 tgatggtatt tatcaaagaa attcaaatca agcattaatt tttgcattac aagctgaact
     2101 aggatattct cccagtgaag caacagggtc ttatggtaat gggacaacta gtaaaactta
     2161 tcctgtgtct gaaggtaact cgggaaacta tgtaagaata attcaatatg gattatatgt
     2221 taatggttat tttgaagacg gtacattcga tggtattttc actaaatata tgggtttaga
     2281 agtactggca ttccgaaaat ttatggtatt gcctgaatat actgaaatag cagatgttgt
     2341 tgtaataaaa ggactattgt ctagcgctgg agatattaga agatctgcag atggcgcaga
     2401 tacttctacg caattaacta gatctcaaat ccaaacattg gttgataatg atataaaaat
     2461 agttggtaga tatcttactg gtactgttgg aattggaaaa gatgaacgaa ataagtatct
     2521 aactacagag gaattgaata atattttttc taaaaattta tctgtgtttc ctatctatca
     2581 agatggaggt gcagcattag cttattttac ttatgatcgt ggcttatcag atggtaagaa
     2641 ggctatagat gcagcaaaaa atctaaatat accattaaca acagttattt attttgctgt
     2701 tgatctagac atgttagggg aagaaattct tgcatatgct ggagaatatt ttaagggggt
     2761 atctgctgta atgtcatata gcgggtatca aacgggtgtt tacggaacaa gaaatgtttg
     2821 ttctcaaata atcaataacg ggtatgcttc gtttgcattt gtttcagata tgtcgactgg
     2881 ttatagtggg aatttaggtt ttcctatgcc tagacaatgg tcttttgatc aatttgttga
     2941 atttaccata ggaagtggca acggagctgt tggtatcgat ttaatcgcta catcgggtag
     3001 agatagcgga tttaatgagt tatcaaatga tactaataat gataattaca ttgccaaata
     3061 caatcagaag gttataaatc agatggtacg tgcgtatcaa tatctatcac aagctggtct
     3121 tgataatcct tggaaccctc atttgacatt ttatagatat gtaaattatt ctggtttgtc
     3181 atgggacatc atttcttctc ctgtaacaga gcatgataga aaaatttatg atgaatatag
     3241 gaataaatta actaatagcg agggcctgta taattatttt atagatccta atactggttc
     3301 aataataggt ttgcctcact taattgtgac gttacaatca cagatgtttc tagccgacac
     3361 aatcaatgat agtgtttctg actttgcagg ttggttagga gatttaatga cttgctgggg
     3421 tgaagttaag aaattaggaa tacaaatgga gcaaggtgta tttgcattag taggtacggc
     3481 tgcaggtgga acatttagtc tagaagattt ataccaagat gttgacgcgg taaatgttta
     3541 taccttagca aaaagaaatt ctgattcaac agctttatcg gctttaaaaa cgtactattg
     3601 tgataagggt gctattgata gatttaatca atttgttaag aacagatttg gttcttcaga
     3661 taaaatcgct tcagtaacaa atagtttatt agaaaatgac gaaacagttg atatcttagg
     3721 tgtagggata atttcatttt tcattgataa aatagttaat aattcacttg ggacagattt
     3781 tacttatctt gattttattg gagatgaggg gaaagttgca aatggatttt ctgaaaaaat
     3841 tattcaattt caagaaaata gaagtaaact taattaagaa tcgtttacta tttaaacagt
     3901 gtccttttcc tttttaaata tatacgataa aataagtata tgaattgtaa aaggacatga
     3961 tgattatgga agaaatagaa tggaataatc tgactcaaaa taataaaaat aattctggat
     4021 gtggaattat ctttatgttt gttatagttt tgggaatagt agtactttta ataactgtca
     4081 agatagtaga ttactttgcg aatactcctg aagaacaaat acagagaaaa cttcctgagt
     4141 ttgaaagagt accaccttat gatccaagag ataattcatt tcaagtaaaa aaattaaaag
     4201 agtctccaaa tgaaatgtgg tttgaagtta cagttgaatc aagcgacttc aaagaaaaaa
     4261 agaaagaata caattatatt gtaaaggcaa aagtatcaaa aagatttatt tttaacccta
     4321 aattagaaat atatagtgtt gtaatagaat aatagtgtta aaaaaattgt aaaagttaaa
     4381 gaagtcaaat tctgatgtga aaaataaaat ataccatgga agacataaaa gaaaaactgg
     4441 ataactagtt tatttaat
//
LOCUS       DAJSBL010000117         4458 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.120, whole genome shotgun sequence.
ACCESSION   DAJSBL010000117 DAJSBL010000000
VERSION     DAJSBL010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4458
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.120"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            191..445
                     /locus_tag="ONT85_002796"
     CDS             191..445
                     /locus_tag="ONT85_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HCR2379717.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEDNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            446..763
                     /locus_tag="ONT85_002797"
     CDS             446..763
                     /locus_tag="ONT85_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HCR2379718.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
     gene            complement(1397..2305)
                     /locus_tag="ONT85_002798"
     CDS             complement(1397..2305)
                     /locus_tag="ONT85_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HCR2379719.1"
                     /translation="MRSSKYTEHYTDTFITFKEIMRTVSNIYHNCVPDKIKNRRNTDQ
                     LKQRDTVIIACVIWGIINGYTSQRATYRAVCSVLFLNGDFPSRSRFTRLSSNLAYTIK
                     IIRYFFIKKLTKGELVGIIDSFPSQLCKPVRNRQAKLLNQIAKVGYNSTKKSYFYGLK
                     IHMIVTKTGFPITYSITNPGVHDVKVLETLSEEANLPNILGDKGYISHKIHEKLALKG
                     ITISVPPRKNMDKSEKLDHSLLGKQRKTVETVFSSLEKLGCQNFNSRSVKGLESRFES
                     ILLAYSVLLSRAQRRFEGTLRYSLGY"
     gene            2524..3894
                     /locus_tag="ONT85_002799"
     CDS             2524..3894
                     /locus_tag="ONT85_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4357 domain-containing protein"
                     /protein_id="HCR2379720.1"
                     /translation="MMDKSNEEQTTLYTKYWRRLEEVFDNSKGMEDFFRYYLAAITGE
                     YSAKHILYQAFKDYWHKERDVSSDAELLQKLVRYAGYFARLYYNKPEGKYSEVLSDFQ
                     SMESMMPAPFVLGLSEWYYHDQFITEDQYIDAIKVVNDYQLRRYFNGDDTSRVSKAFP
                     SYLKSVRKYAKANGYENIVDIVIYVLVTRNQSNNMALPSDKALKSNLLNANAYAMRLA
                     RWLLEKIENRENSATLDMSNLSIEHIMPQTSTSYWEEKAGTSGEEYTGLVNTIGNLTL
                     VTNPDNSAAGNKDFETKKKIFEDTLHIRMNKDLYELTEWTSSDISARSEALINELITM
                     YPYLRSSGDYEHDGNREIFLEAQGIKATGYLNEDETVIIHSGSEIYSKIKDIASDSLD
                     ETRQELLDNGIIEETIGGLQFVQDYTASSVSNAAALLLGGSRNGWDYWKYDNGISIND
                     SLRNKK"
     gene            3915..4199
                     /locus_tag="ONT85_002800"
     CDS             3915..4199
                     /locus_tag="ONT85_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2379721.1"
                     /translation="MDKNKLRYAILKLRDEKKNPFIELKGQVPEEDILEQTKFLSRNG
                     LLESMVWADNTVHIWGSVSLEGEKYLVENSGLAKAYALAKEVKNWIPFWG"
BASE COUNT         1510 a          673 c          884 g         1391 t
ORIGIN      
        1 gatgttaacg gaagtgtatg gtgagtagtt cactcgtatt ttgatatcgt ttgtatatgt
       61 tttcagcgtt ctaagtggct tattttgtcc aataataata tggagtggaa tcccttaaga
      121 aacaccgttg gcaattgttg ttataacatg ttataattta ttcatcaaaa actaaaagga
      181 gtgtgataac atgggaaaag aactaaaggt tagaaagatc ggcaatagcg tgggagtgat
      241 ccttccaagc tctcttggat taaaaagcgg agacattatc caagccaaac aagaagacaa
      301 cttattcata ttagatacta ctcaaatagc aaaagaacac gatcgcaaat tgattgaaga
      361 aagtttccaa gactttgaga aaggtttaac agtttcagaa attgaaatgg taaaagcttt
      421 cggaaaatac ggctggagtg aatagatgaa agagtacacg ctagtttatt ctaaaagttt
      481 tcagaccagc cttgaaacga taatagaaga atgggaaaat gagcttgctc tttctccagc
      541 cagcataaaa agatttgtag gtgcgatcca gaaggcttta gaactaacca aaaattttcc
      601 aactatgcat gaggaagttt ccgaaatcta tggaatggat atcccaacat atcggatcgt
      661 gatcggcaaa agctatgcct tattttatcg gatcgaccat gaaaaaaaag agatacttgt
      721 aggacgtatt ttccaatcta aacaaatgaa actaggcttt taacaataat taggaaaaag
      781 tcgctcatcc tctacaggtg ggtgattaat gccgctcgat atggttgtta accgtcggtt
      841 actcgaagat tgatatataa gacttagtac ctttttattt tgctccatgt tatggccgaa
      901 ggtctaaaga tacaatcatt agtctagaga aaaattaaga accttaggaa ctacggggat
      961 cgcttggtga ataagagaca ctgctattag tgaaaaaata agatatcaag tacgctctat
     1021 tcccaaaaat ttcgtgactt tactcatgag aggttcaagt agtatatttt taatcaaaat
     1081 tgacgtaact tttaataaat actacctaaa taaggtagta tttatttttg tgttttaaaa
     1141 gaggaagtcc aatatatcta tgggaaactt cttaaatatt atgttggtac atgaaaatat
     1201 acttccctgc tagactattt acaacatttt actttgtacg tattcttgtt ttaaaaggaa
     1261 tgggataaaa tataggtaca ataactattt gatgctaaat ataattaatg gaggagagat
     1321 agtgaacata cttaatgaaa aaagatatgt atggtttgct ggtaaaaaat atattaaccc
     1381 gtggtgctag ttaattttaa tatcctaaag aatatctcaa agttccttca aaacgtcgtt
     1441 gtgctcgact taatagaaca ctgtaagcca gtaatatgct ttcaaatcta ctctctagcc
     1501 ctttaacaga acgtgagttg aaattttgac aacctaattt ttctaaggaa gaaaaaacag
     1561 tctcaactgt ttttctttgt tttccaagta agctgtggtc tagtttttct gatttatcca
     1621 tgtttttacg tggcggaacg gatatagtaa tgcccttaag ggcaagcttt tcatggattt
     1681 tatggctaat atatcctttg tcccctagga tattgggaag attcgcttct tccgataaag
     1741 tctctaacac tttcacgtca tggacacctg gattcgtgat tgagtaagta atcggaaagc
     1801 cagtcttagt aacgatcata tgaattttaa gaccataaaa ataagatttt tttgttgagt
     1861 tatagccaac tttggctatt tgattcaata gttttgcttg tctatttcta actggtttac
     1921 ataattgact aggaaaacta tctataattc cgactagttc acctttggtg agcttcttga
     1981 tgaaaaagta tcgaataatc ttgatagtgt aagctaagtt tgaacttaag cgagtgaatc
     2041 gactcctact aggaaagtca ccattaagaa ataagacgga acaaaccgct ctatacgtgg
     2101 ctctttggct agtatagcca ttaattatgc cccatataac acaagcaata atcactgtat
     2161 cgcgttgctt cagttgatca gtatttcttc ggtttttaat cttatctggt acacaattgt
     2221 ggtatatgtt agaaacagtt ctcataattt ccttgaatgt tataaaagta tctgtataat
     2281 gttcagtata ttttgatgaa cgcatatgta gtcctcattt cttgggagtt ttgagactac
     2341 tatatgcgtt ttttgttgat ttaccaacat gtttagacat tttcctctag gaaaaaatta
     2401 actagcacca cgcgtattgt ttatattaaa ttagatgacc gtgataatgc acagcaaatt
     2461 tttgaaagta tcaattctac cggtgaaaga ttgactgcct cggatttgat tcgaaacttc
     2521 atcatgatgg ataaatctaa tgaagagcag actacgcttt atactaaata ttggcgtagg
     2581 ttggaagaag tctttgataa ttctaaagga atggaagact ttttcagata ctaccttgct
     2641 gcaataactg gggagtattc agcaaagcat attctttatc aagcattcaa agactattgg
     2701 cataaagagc gcgatgtatc aagtgatgcc gaattgctcc aaaaattagt gcgatatgct
     2761 ggttattttg cgagacttta ctataacaaa cctgaaggaa aatactcaga agtattgagc
     2821 gatttccaaa gtatggaaag tatgatgcct gcaccttttg ttttgggatt atctgaatgg
     2881 tactatcatg accaatttat cactgaagac caatatattg atgcgattaa ggtagtgaat
     2941 gattatcagc ttaggagata ttttaatggg gatgatacaa gcagagtgtc aaaagcattt
     3001 ccttcatatt tgaaaagtgt taggaagtac gctaaagcta atggatatga aaatatcgtt
     3061 gatattgtaa tttatgttct tgttactaga aatcaatcaa ataatatggc acttccatct
     3121 gacaaggcat tgaaatctaa tctattgaat gccaatgctt atgcgatgag attggcccgt
     3181 tggttacttg aaaaaataga gaatcgagaa aattcagcaa cacttgatat gagtaacttg
     3241 tccattgaac acatcatgcc acagactagt acttcatatt gggaagagaa ggctggtaca
     3301 tcgggggaag aatacacagg attagtaaat accattggta atttaacact cgttacaaat
     3361 cctgataatt cggccgcggg aaataaagac tttgagacca aaaagaaaat ctttgaagat
     3421 acccttcata ttcgtatgaa taaagatttg tacgaattga ctgagtggac atcatcggat
     3481 atttctgcca gaagtgaagc tctaatcaat gaactgatta ctatgtatcc ctatttgaga
     3541 tcgtcgggcg actatgaaca cgatgggaat agggaaattt tcttagaggc gcaaggaata
     3601 aaagcaactg ggtatttgaa cgaagacgag accgtgatta tacattcagg ttcagaaatt
     3661 tattctaaga ttaaagatat tgcatctgat tcgctagatg aaactcgcca agagctgttg
     3721 gataatggaa tcattgaaga aacaattggg ggactacaat ttgttcaaga ttacactgcc
     3781 tcatctgttt cgaatgcagc agctctgcta ttagggggta gccggaatgg ttgggactac
     3841 tggaaatatg ataacggaat aagtattaat gattcgctaa gaaataagaa atagagcata
     3901 gaggtggtgt taggttggat aagaacaaac taagatatgc aattcttaaa ttgcgtgatg
     3961 aaaaaaagaa tccttttatt gagcttaaag ggcaagttcc agaagaagat attttagaac
     4021 aaactaaatt tctttcgcgc aatggcttac tagaatccat ggtttgggcg gataacactg
     4081 ttcatatctg ggggagtgtt tctctggaag gggaaaaata tttagttgaa aactcaggtc
     4141 ttgctaaggc gtatgcattg gcaaaagaag ttaaaaattg gattccattt tggggctaag
     4201 ataagttttt aaacggagaa ggtagcaaat cataattggt ttgttgcctt ttttgattgc
     4261 ccaaaaagaa aaatgacaaa gtatagagat aaatgacata gtacacggga aaaatgacat
     4321 tgtatcaccg acaacgtcta aatagatgtt gatatatata cgttccgtac aaataattat
     4381 aaaaatttaa atgatttgag tagaagtatc agtgaatatc tcaaggggaa tagctatctc
     4441 aagattcgat tttcacat
//
LOCUS       DAJSBL010000118         4433 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.121, whole genome shotgun sequence.
ACCESSION   DAJSBL010000118 DAJSBL010000000
VERSION     DAJSBL010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4433
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.121"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(543..2048)
                     /locus_tag="ONT85_002801"
     CDS             complement(543..2048)
                     /locus_tag="ONT85_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HCR2379722.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(2045..2731)
                     /locus_tag="ONT85_002802"
     CDS             complement(2045..2731)
                     /locus_tag="ONT85_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR2379723.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="ONT85_002803"
     CDS             complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="ONT85_002803"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HCR2379724.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         1239 a          919 c          729 g         1546 t
ORIGIN      
        1 tctttttgtt ttaacaactc tttaatctta tcagattcag aagagaatgt caacaacttc
       61 tttgtttatt ttttctaaac gtctttgcca ctcattagca gcgacttgat tatcatacca
      121 tctttattgt tatttgtcaa taactttttc gaagtttttt tcgataaaat caagctttat
      181 tagctatctc atccaaacga cttcgttatt ataacatgac atcaaaagtg cgtcaacact
      241 attttttata atttttagtg acaccttaaa ggaatcattt agaacaaata ataatatagc
      301 aatctcttta taaaattgca atagaaaatt ccctattttt atttattatt tttaaacacg
      361 aatattacag cattttattg ttttttcgtt catctaaaag atacttacat ttaattaacg
      421 gatgttattc tatttaattg ttcgctagtt tttttataat gacatccctg aatttctcac
      481 ttgcttgtat tttgtacctt attctttgtt ggatatataa aaaaacaagt aaagcacatg
      541 ctttacttgt cttctgcctt tgcgatagga ataaagattc taaaaattgt cccttgccct
      601 actacacttt ctgctaagat acgtccttta tagttttcaa ccagttgttt agcgatagac
      661 aaccccagac catttccacc cttcgttcta gctcttgctt tatccactcg ataaaaacga
      721 ttgaagatct tattaaggtc ttctttgggt attccttcgc caaagtcctg aatcgcgatc
      781 tcaaattcat tcagagtgga agaaatagag atatggactt cttttcgtgt agttgaatac
      841 tttactgcgt tatccaatat gataatgatt aattgttcaa agtgattacg ataaatttgc
      901 agggtcactt cttttgtcaa atcatcatct aaagtgatca catagtctgg atataatatc
      961 ttgaaattat tataaacttg ataggtgact tcttttcctt tagttgtttc attcgcataa
     1021 tgaacatcta cttgttctgc tcttgataag tctagcatct cttgaactaa gcttttcata
     1081 cggctgattt cttgcatgct ggcgcttaat gattcttcta atatctcagg atcatctttc
     1141 ccccaacgat ttagcagact aagatgccct tcaataattg caacaggtgt ccttaactca
     1201 tgagatacgt cttccacgaa ttgctcttgt tgttcaatat acaaacgcat acggtcaagc
     1261 atctcattga atatttcagc taaatctgcc aattcatcat ttgtgtcgat ttcaggcatg
     1321 tggatatctg attgcggatc tttacgtata gtatccattg tatctctgag taccttgagt
     1381 ggttttaaga aataagaaga taaaaagaaa cctaaaatac tacttaagat caaagaaaca
     1441 acttctaaaa caataagagt caataatagc ttatttcgaa tatcatagaa agaagataat
     1501 tcatagaagc tttgcgcata gccgatcttt tctcttgtac ctttagaata tataggctgg
     1561 atagataaaa agccagtttt tccttctagt gtaacgatcg ttggaacttt acgtgtagtc
     1621 tgaatcaggt ttcgactgtt ttcatgggtt ttaaaaatca agttctcgtc caaatcataa
     1681 acataaagat ccattgatgg ctgccctagc tcagctataa aggaatcgat cttcattaag
     1741 tttccttcga ccagtgaagt atgatctacg acctcatctc cgtcatttgt cgctttcgtc
     1801 agattacgat acgtatttac taatgtcagt tcttcatccg agttagccaa acgagaagct
     1861 acttctgaaa tcgtacgctc tacattgttt ctttcttttg ccacaatcag attgatagat
     1921 gatttatagg taatgacagc gaaaattgta aacacaacga atatgaagaa agaactcgca
     1981 aaggcccact tgattgtcaa ggagggccct ttcagttctc ttttttccga ttttttcggc
     2041 tttatcacga gcgcatcaca taacctgttc cgcgtacagt ttggatatag ctttcctccc
     2101 cgggaacgtc aattttgttg cgaagataac gaatatatac gtccactacg tttgtttcaa
     2161 cttctgtttc gtatccccac actttattta gtaaaacgtc acgtgctaat accacgttca
     2221 cgttttccat taaagtaagc agcaactcgt attctctttt tgttagttca attacttcat
     2281 ttccgcgacg tacgacacgg ttttcttttt cgatagtcaa atcgcgataa gtaatagtag
     2341 tttgtttagc cacattttta tcgccttcaa tatcgattcg acgcaacaat gcacgaagac
     2401 gtgctaataa ttcttcgata gcaaatggtt tgacgatgta gtcatctgct ccgtggtcaa
     2461 gtccagatac acgatcgatc acagaatctc ttgcagtcat cataatgatt ggtgtatttt
     2521 tgacttggcg tacacgtctg cagacttcta aaccgttcaa ttctgggagc atcagatcca
     2581 acaatatggc atcccactca ttatttaatg ccgcatctaa accagttcgg ccgttgtagt
     2641 ggacttctgc gttataccct tcatgcttca actctagctc gacaaatcgt gccaagttct
     2701 tttcatcttc aatgattaga atattgctca tttattattt tatcctttct attctactgg
     2761 ctttttaaaa actctatcat tataccgata atatgcttaa aaatctatag tcattttatc
     2821 atggatagca attttaaaaa agagctgaag tctactcata acaatcgact tcagctcttt
     2881 cgtcctcttt ttattttctc atttcctaac agccgaaaga ctattattct tcatggtacc
     2941 atgagtaatg gaagattcct tctttgtcga cacgctcata ggtatgtgcg ccaaagtaat
     3001 cgcgttgggc ttggattata tttgcaggca gacgttctgc tctgtatgaa tcgaagtaag
     3061 cgatggctga tgagaaagtc ggtacaggta cgccagcttg gacagccaag gcaacgacgt
     3121 ctctgactga ttgctgatat tttttcgtga tttcaacgaa gtaatcatct agcaataaat
     3181 tttcgatctc aggatttttg tcataggcat ccgtaatttt ttgcaagaat cgagctcgga
     3241 tgatacatcc agcgcgccag atttttgcga tttcaccaaa tggcaagtcc caatcaaatt
     3301 cttttgaagc gacacgtaat tgagcaaatc cttgggcata actcatgatt ttactgaaat
     3361 acaacgcttc tcggattttt tcgaccaatt cttttttgtc tcccgtgaac tcaaaatcgt
     3421 tcgtacgaga caaaatcttg cttgcttgta cgcgttcttc tttataagca gaaatatagc
     3481 gggcaaatac agattcagtg atcaatggaa gtggcacacc taagtcaagc gcgctttggc
     3541 ttgtccattt acctgttcct ttattcccag ctgcatcaag gatcacatct actactggtt
     3601 tacctgttcc ttcatcgtct ttacgtgtca gaatgtcagc agtgatttct atcaaatagc
     3661 tgtctaattc gccttgattc cattctttaa agatatcagc catttcttct actgaaaggt
     3721 ttaaaatatt cttcattaag tcataagatt cagcaatcag ttgcatatct ccatactcaa
     3781 taccgttgtg taccattttc acataatgtc cggctccatt tgggccaata tatgtgacac
     3841 atggtgctcc gtcttctgct tttgcagaaa tttgctctaa gattggtgca actagcgcat
     3901 aggcttcttt ttgtccacca ggcatgattg aaggtccttt taaagctcct tcttcaccac
     3961 cggaaacgcc tgtaccgata aagttgatgc cagagttggc taactcttca ttacgacgca
     4021 tcgtatcttt aaagaatgta tttcctccgt caatcaaaat atcgccttta tcaaggtgag
     4081 gaagtaactc ttggattgtt gcatctgttg ccggtcctgc tttgaccatt agcaagatcc
     4141 gacgtggttt ctcgatagat tctacaaatt cttcgattgt atatgttgct ttgaaatttt
     4201 tatctgggtg ttcttctact acagcggttg ttttagcccc tgtacgattg aataaagcca
     4261 ctgaatagcc gcggctttct atgttcaagg ctagattttt acccataacg gccataccga
     4321 caacgccgat ttgttgtttt gacatatgaa ctcctcctac atacgggatt tctccctaat
     4381 ctcttacatt atagcttaac acggctacat tgcaaactaa agaacaacat att
//
LOCUS       DAJSBL010000119         4425 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.122, whole genome shotgun sequence.
ACCESSION   DAJSBL010000119 DAJSBL010000000
VERSION     DAJSBL010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4425
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.122"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            690..1658
                     /locus_tag="ONT85_002804"
     CDS             690..1658
                     /locus_tag="ONT85_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HCR2379725.1"
                     /translation="MDVAKTVNAIVEKNDGEISGIYLVACGGSLVDMYVSDYFLKAEA
                     SKCITGLYTANEFNHVTPKQLDDKSVVILCSHGGNTAETVKAGEVAQARGAVTVGLTH
                     NKTADLLKVSDHSFLYEWGDDAKIVNNPMAIILDLTVSLVNVTETFEHYGKFREGMKV
                     IDTVIENGIKQVQDRIKVFADKYKDETMFYILGSGPSYGHAYGFSICSLMEMQWLDST
                     SVHSGEFFHGPFEVTDKDTNFILLMSIGRTRALDERAKRFLDKYAERVEIVDAKELGL
                     DMISDEVSEFFNPILFYSILSEYRSALADVRNHDLDVRRYMGKVEY"
     gene            1669..2484
                     /locus_tag="ONT85_002805"
     CDS             1669..2484
                     /locus_tag="ONT85_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HCR2379726.1"
                     /translation="MKVLGLGDNVVDKYENLGIMYPGGNSLNFAVFAKKLGHESSFMG
                     VFGSDEEAAHVLSAIKEIGLDNSHSRFEKGENGCARVKIDEGDRIFLGSNSGGVTREY
                     PIQLTEEDREYLNQFELIHMGLYSHVNHLLEELQNVSGKISYDFSDDFTEEEVQRAID
                     KVDFGFFSIEDFSDEEVKQFLMKHFCERNDVLIATRGEKTAIGYDGAKFYEVNPVLEE
                     PVDTMAAGDSFLTAFLLYFLEGDDIVTAMKKGNEFAGKSCLVDGSFGFGIHYD"
     gene            2552..3262
                     /locus_tag="ONT85_002806"
     CDS             2552..3262
                     /locus_tag="ONT85_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR2379727.1"
                     /translation="MLDTNAQLPLYLQLKEILKNKINTGEYPENTKIPAESELIESYE
                     VSRITVRKAVEELVEEGYLLKKQGKGTFVNRHKVFRKIEYIMGFSESCKSNGFTPTSI
                     VLERKIIPATEGMAKKLDIAQGDDVLYIQRKRMADNIPIMLENNYFSYERYSFLMEEE
                     LTDSLYEVLKVQGTLAINPGETALEIVTADDQIAKIMEVAVGTPFFFMDTLINDQNNI
                     PIHVGNQYYLGEVYRFYL"
     gene            3298..>4425
                     /locus_tag="ONT85_002807"
     CDS             3298..>4425
                     /locus_tag="ONT85_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HCR2379728.1"
                     /translation="MKQILRSKMIFDGEQPPFKGYIVINEGIIEKVENNWNYSDLLNE
                     NTELIAYDDQFVMPAIHDNHVFFSGYLAMNAGIDLSDTSSANEAVERIKAVMNHKKAD
                     EPIYAHGWDAALWKTDPSEELLNEINHFGPITAIDKNRSHCWMNAIAKQTYGFTEKET
                     SAESRVLLIKEMLANKKLLAEVYAEFEALLLSKGVISMKEIVFDDAEFLSNIPQRKIL
                     SNLYVQAVQEPLQHELLYEYKEKVFPEKVRFGGVKIMVDGVVADRSGDIYGVYQSGVA
                     SPEIDYEAIKAEVAAFNEVEIPCCLTTEGDKAGYKAASILVEFGKRLPEGVFNSISDL
                     EMVTQKTAEKMNEGQVVAEVYPQILGLNQTLEEAYMSTVIKN"
BASE COUNT         1490 a          661 c          921 g         1353 t
ORIGIN      
        1 atacaaataa ttcaccaaaa aggtgagtaa tgccattttt gaaaattgag cttgttttat
       61 gtctacatct gtatccgtat ataatccaaa ggattataaa ctcagaagag tctttatttt
      121 ttctgaatat aactactgta tcaaaaacaa tcgattatga gacaaaacaa aatgacctct
      181 tttatttaag aaaggaggtc attttttgta tcataaattg tattaaaatc aatgtatcac
      241 tttttaatca gttttaagac cataaattgc gtactaaaag aaattctgca attcaaaata
      301 aagcatttta agccattttc aaatttaagt gcacccatct acccgtcaaa tcactttatt
      361 ttccactcca gtagtttcct aattcaactg tgtgcaaaaa cgatttcttc taaacaaatc
      421 aagacaaact tataaacctt catcccagca ccataattat ttaaatcata ggcatttttc
      481 gaaattagta tatgtactca agtttcactg cagattattt atatttttta gagaaataaa
      541 tttttcctat tgacaaacac aaagaaagcg gatacaatgc aattatgtca catgacgtta
      601 tatgacgtta tgtgatatct ttacatttaa tcagttactt ttattttgga taaaattaac
      661 atttttaaaa ttttaaggag gaaatgttga tggatgtagc aaaaacggtt aacgcaattg
      721 tagaaaaaaa tgatggagag atttctggaa tttatttggt ggcttgtggc ggttcgctgg
      781 tagatatgta tgtctcagat tattttctta aagccgaagc atcgaagtgt attacaggct
      841 tatatacagc gaacgaattt aaccatgtaa ccccaaagca attagatgat aaatctgttg
      901 tcattctatg ttctcatgga ggaaatacag cagaaacggt aaaagcagga gaagtcgctc
      961 aagctagagg agctgtgact gttggtctga ctcataataa aactgcggat ttattaaagg
     1021 tttctgatca tagtttcctt tacgaatggg gagatgatgc aaaaatagtc aataatccta
     1081 tggccatcat tcttgattta actgtatctt tggtcaatgt taccgaaaca tttgaacatt
     1141 acggtaaatt cagagaaggc atgaaggtta tcgataccgt aattgaaaat ggcattaagc
     1201 aggtgcagga tcgtattaaa gtgtttgctg ataagtacaa agatgaaaca atgttttata
     1261 tcttaggtag tggaccatca tatggacatg cttatggttt ttcaatttgc tctttgatgg
     1321 aaatgcaatg gcttgattca acttctgttc attctggaga attcttccat ggaccttttg
     1381 aagtaacaga caaagatacg aattttattc tcttgatgag cattggacgc actcgtgctt
     1441 tagatgaacg ggcaaaacga ttccttgata aatatgcaga aagagtagaa atcgttgatg
     1501 caaaggaact aggattggat atgatatcag atgaagtttc tgaattcttc aatcccattt
     1561 tattctacag tattctttca gaatatcgtt cagctttagc agatgtacgc aatcatgatt
     1621 tagatgttcg tcgctatatg gggaaagtgg agtactagga ggattaaaat gaaggtatta
     1681 ggcttaggtg ataacgtcgt cgataaatat gaaaatctgg gaattatgta tcctggagga
     1741 aattcattga attttgcagt ttttgcaaaa aaactgggac atgaaagtag ctttatggga
     1801 gtttttggct cagatgagga ggcagctcat gttttatcag caataaaaga aataggactg
     1861 gacaacagtc actctcgctt tgaaaaaggc gagaatggct gtgccagagt gaagattgat
     1921 gagggcgacc ggattttcct tggcagcaat tcgggaggtg ttactcgtga atacccgatt
     1981 cagcttactg aagaagacag agaatattta aaccagtttg aattgattca tatgggatta
     2041 tatagccatg tcaatcatct attagaagag ttacaaaatg tttctggaaa gatttcttat
     2101 gatttttctg acgactttac ggaagaagag gttcagcggg caattgataa agttgatttt
     2161 ggcttctttt ctatagaaga cttttccgat gaagaagtaa agcagttttt gatgaagcat
     2221 ttttgtgagc gaaatgatgt attgattgca acccgaggag aaaaaacggc tattggttat
     2281 gatggagcaa agttttatga agtgaacccc gtgctagaag aaccagttga tacaatggca
     2341 gcgggagatt ctttcttaac agctttccta ctttattttc tcgaagggga cgatatagtc
     2401 accgcgatga aaaaggggaa tgaatttgct gggaagtctt gtctggttga cggttcgttc
     2461 ggctttggta ttcattacga ttgagcttgt tttaaattcc tgcatttgat aacctaaaga
     2521 aagaaatccc ggtaaagtgg aggttaaaaa aatgctggat acaaatgcac aattaccatt
     2581 gtacttacaa ttaaaagaaa tattaaaaaa taaaatcaat acaggggagt atcccgagaa
     2641 tacgaaaatt cccgctgaat cagagctgat agaaagttat gaggtcagcc gaatcacagt
     2701 gagaaaagca gtagaagaac tggtagaaga agggtaccta cttaaaaaac aagggaaagg
     2761 aacttttgtc aaccggcata aagtttttcg taaaattgaa tatattatgg gtttcagtga
     2821 gagttgtaaa tctaatggtt ttacaccgac tagcattgtt ttggaacgca agatcattcc
     2881 agcaactgaa gggatggcaa aaaaactcga tatcgctcaa ggtgatgatg ttttatatat
     2941 ccagagaaag cgaatggcgg ataacatccc cattatgtta gaaaacaatt atttttctta
     3001 tgaaagatat agtttcttaa tggaggaaga gctgactgat tctttgtatg aagtacttaa
     3061 agtccaaggt accttagcca tcaatcctgg ggaaactgca cttgaaattg tcacagctga
     3121 tgaccaaatt gccaaaataa tggaagtagc tgttgggacg ccctttttct ttatggatac
     3181 tttaatcaac gatcaaaaca acataccaat tcatgtaggt aatcaatatt acctgggaga
     3241 agtatatcgt ttttacttat gatttcctgt ttggatttca aggagtagat tttatttatg
     3301 aagcaaatat tgcggtcaaa aatgattttt gatggagagc agccaccttt caaaggttat
     3361 attgtaatca atgaagggat cattgaaaag gtggaaaaca actggaatta cagtgatcta
     3421 ttaaatgaaa acacggaatt aattgcttat gatgatcaat tcgttatgcc agcaattcat
     3481 gataatcatg tatttttcag tggttatttg gctatgaatg ctggcataga tttaagcgat
     3541 acatcttcag ctaacgaggc tgtagagcgt attaaagctg taatgaacca taaaaaagca
     3601 gatgaaccaa tttatgctca tggctgggat gctgccctgt ggaaaactga cccatcagaa
     3661 gagctattga atgaaataaa ccactttggt ccaatcacag cgatcgacaa aaatcgcagc
     3721 cattgttgga tgaatgctat tgctaagcag acttatggtt ttacagaaaa agaaaccagt
     3781 gctgaaagtc gagtattatt aattaaggaa atgctagcaa acaaaaagct tttagcagag
     3841 gtttacgctg aatttgaagc attactgctt tcaaaaggtg ttatatcaat gaaagaaatc
     3901 gtttttgatg acgcagaatt tctttctaat atacctcaga ggaaaatact tagtaaccta
     3961 tatgtgcagg cagtccaaga acctctacag catgaacttc tatatgagta taaggaaaaa
     4021 gttttccctg aaaaggtccg ctttggtgga gtgaagatca tggtagatgg tgttgttgcg
     4081 gatagaagtg gagatattta cggtgtttat cagtcaggag tagcttcacc cgagattgac
     4141 tatgaggcaa ttaaagcaga agttgctgca ttcaatgaag tagagattcc ttgctgtttg
     4201 acaacggaag gagataaagc gggttataaa gctgcgtcaa ttcttgtcga atttggaaag
     4261 cggttacctg aaggcgtttt taattcgatt agtgatttgg aaatggttac tcaaaagaca
     4321 gcagaaaaaa tgaacgaagg acaagtagta gcagaggtat atccacaaat tctcggcttg
     4381 aatcaaacgt tagaagaggc ttacatgtca actgttataa aaaat
//
LOCUS       DAJSBL010000120         4348 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.123, whole genome shotgun sequence.
ACCESSION   DAJSBL010000120 DAJSBL010000000
VERSION     DAJSBL010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4348
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.123"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(387..1853)
                     /locus_tag="ONT85_002808"
     CDS             complement(387..1853)
                     /locus_tag="ONT85_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR2379729.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKKITEPETVVQP"
     gene            complement(2144..4228)
                     /gene="cadA"
                     /locus_tag="ONT85_002809"
     CDS             complement(2144..4228)
                     /gene="cadA"
                     /locus_tag="ONT85_002809"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HCR2379730.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQETKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTTEGLMTVNPEDVQIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKKFMQYIASAEQFSTHPIAQSVLEGYAGPLLP
                     TTNIQEFAGEGILAEVDGKQVLAGNHKLMERFEISFPLSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKQAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQRNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLALGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKKEDE"
BASE COUNT         1309 a          927 c          697 g         1415 t
ORIGIN      
        1 aataaatggt gttcaatcag ctgaccttca gcattagctc aaaaagcggc aaaatatgag
       61 gagctatttt tccgtttgtt tctctaatac tcaggtcaaa tcgctgattt tacaacctct
      121 tccttaaacg gtgttccaaa gcttgcatct gcggtaataa gcccagccaa acgaaaaata
      181 tgaggggcta tttttgtttg gctgttctta ttacttgatg caggacagct ttttcacaac
      241 ctctgattca cggtgttcca gaagtagctt cttcgaaaaa caagccgaaa cttcacaaaa
      301 atttgagaag caattttcgt aaattccttc ttatttcttg aagctgacca cttctgtcac
      361 aacctcttgt aacaagtatt tctattttaa ggctgaacca ctgtttctgg ttcagtgatt
      421 tttttgtttt tgtctcgacg agcaattgct ggcaagatca tacctaataa gatcaagacg
      481 attggtgtga tgatattcgt aaataattgg aaccaccatg ctttaggatc agcagtataa
      541 tctacttttg gcaccattcc taggatacat gcaaacgcag tgaaggcaaa gcaccaagca
      601 ccaaagataa aaccgatttt tggattttta acaaatttat attcagaatt gaatttctta
      661 taagctttat tcaacatcat atatgccaag aacacccata ggtaacgcat tggcatgaca
      721 actgagttca agttcgtcaa ccattttact aattcattca tatctttgat accaaaaatc
      781 ggcaacaaaa taatgattcc tactagtata cctgtcagaa gataaccatt caccaatgta
      841 cctttacgat tacgtttacg tagccagttt ggtacaaatt ctggatccgc atctgctaac
      901 aagattttta gtggtgcatc aattgaaaag gctaatgcgg agatttgtcc aatcatgttt
      961 gtcaatgcgt aaatgatcat caatgagtta ccgataccat agtaattacc taaaatttga
     1021 aaagcactgt atgccccatt tgccatcaag tctttaggaa tattatcact agcaaacagc
     1081 atacccattg cgactgagcc taatacagca caaatcccta ccattgctgc taagaagatc
     1141 atccctttag gaaattcctt tgcaggattt cttgtagagt tgacataagg ggatattttt
     1201 tccgcccctc caactgcaaa tactagcatc gaaacggttg tgaaataact gaaatcaaat
     1261 gttggaatat atgttttcac ttgattcata tgtggtgtgg caaattccat cgttggattg
     1321 atgaacggtg ccgttacagc taaaagaata aataagatcg acatgacgaa catcgctgtt
     1381 ccggccagtc cgccaatcac tttcaaagta gaaagtcctt ttgttgataa atataaaaag
     1441 gcaagaaata taataagtga aataactgct accatttgta ctgacatcgt atttacgata
     1501 tttccatttc cttggactgc ccaaccacca gcaatcagaa ttgcttgagg cttttgtgcc
     1561 aaataaggaa tatggacgac ccagtaagtc caagcggcat agtatgccag tcgtttatta
     1621 gatgtcgtat tttcgaccca tgaactgacg cctcctttac tatccttgaa ggctgatcct
     1681 aattgtccaa cgatcaatgc gtatggaata aaataaatgg ccaaaatcaa tagccaagag
     1741 gtgacaactg atactccttg ctgagcatag ttattaacta cattccccat gccccaaacc
     1801 atgttaaatg caattaacgc aacagtaaac cagcgtaatt gtttgttgtt cattcgttct
     1861 ctcctttatg ataaaaattt gttatccaga tgtaatcata ttcaattttt aacaaactta
     1921 caagacagtg ttattttatg tgtgtattct tttatttaat acacatcgtt ttttcatatt
     1981 ttttccatca aaaaaataag ttattttctt ttatttcatt gaccttattg aatttcaagc
     2041 tattttgaat tttctgcaaa attaaaaaca ttagttattt tataatccta tctattttct
     2101 ttacattttt cattaagaaa aggatataaa aaaaagagga cgttcattcg tcctcttttt
     2161 ttacatgcaa gcttcgcatg gcattcaaaa cagctaatac tgtcacccca acatctgcaa
     2221 atactgcatc tcccattgat gcaaaaccaa aagcacctaa ggcaagtacc aaaattttta
     2281 tcccaatagc aaagacaatg ttttgtttga cgattttcaa tgttttcttt gctaaatgga
     2341 ttgcttctgc aatcttcgat ggttgatcat tcatgatgac tacatctgct gcttcaattg
     2401 cagcatcgct tcccaatcca cccattgcga ttcctacatc tgctcttgct agcaccggtg
     2461 catcgttgat tccatctccg acaaatgcgg tctttttatt gtttctttgt aagatttcct
     2521 ctaaccgctg cactttatct tcaggaagca attcgctata tatcttgtcc acgcctactt
     2581 gcttcccgat atgatcagca atttttttcg agtcgcccgt caacataact gtgttcttca
     2641 caccagcctg ttttaattga accaatgcgt ctacggcatc ttctttcaag gtatctgcaa
     2701 taacaagata accactgtaa ctttgatcga tcgctagata aagcaatgta ccgatttctt
     2761 gacttaaagg aaaagagatt tcaaatcttt ccatcaattt gtgattacct gctaatactt
     2821 gtttgccatc aacttcagct aagatccctt cacctgcaaa ttcctggata ttagttgttg
     2881 gtaaaagcgg acctgcgtag ccttctaaaa cagattgagc aatcggatgt gtagaaaact
     2941 gttctgcact cgcaatatat tgcatgaatt tctttggatc gatttttgta tcgatcgtct
     3001 ggatcgaaaa gtcacctttc gttaatgtgc cggttttatc gaataccact gtctcggttt
     3061 gcgcaagcaa ctccaagtaa tttgaacctt ttactaatac accgatttta cttgcgcctc
     3121 cgataccgcc aaagaaacta agtggcacgg aaatcactaa tgcacatgga catgagatca
     3181 ctaaaaatgt caatgctcga tagatccatt cagaaaacgc cgctccagaa acaacaagtg
     3241 gaggtaaaac agctaataaa acggccaatc ctacaacgac tggtgtgtaa tagcgcgcga
     3301 atttagtaat gaaattttca gctggtgctt ttttactgct ggcattttct actaattcta
     3361 ataatttgct gatagtagaa ttttcataag atttttctac ttgaactaaa aatggtttat
     3421 ttttattgat aaatccgctt aatactgttt caccaacata tacagattta gggactgatt
     3481 ctcctgtaag tgctgacgta tcgactaatg attctccttc gatgatcgtt ccatctaacg
     3541 gtacacgttc accaggcttc acaagtacaa actgaccgat ctgcacgtct tcagggttca
     3601 cggtcatcaa tccttccgtc gtctgcacgt gagctgtttc tggacggatc gccatcaatt
     3661 cgcgaatcga ttttcttgaa tgactgactg ccaatccttg gaaatactcg ccgatttgat
     3721 aaaatagcat cactgctacg gcttctggat attcaccgat caacatagcg ccaatcgtag
     3781 caacagacat aagaaaattc tcatcaaaga tttttccttt gacgatattc atcactgcct
     3841 tcttcactac atcatatcct atcagcagat aaatcaataa ataccctatc aagcgaattg
     3901 gttcattaaa atcaaataat agcaataaag cgatacctgc aagggaaaat aaaatccgga
     3961 acagcgtatt tctctctttt tcatttccat gttcagatac tttttcctta tctatttctg
     4021 ttactttgac atctggttct aattccttca ctactttttt tgtttcttgt tcaatcgtag
     4081 ccaaatgatc ttcctctgcg tcaatcgtta gttttccaga agtgaagttt accgttgctt
     4141 ccttgactcc attgatcttc tgaactcctt tttcgatttt cattgcacag ttcgcacaat
     4201 ctaaaccttc taatctgtaa cttttcattt taatgacctc ctcgcctcaa catatgaaca
     4261 tctcttcata actaattata aatgaatggt tattcatatg tcaaggtatc cgaaagaaaa
     4321 aatgaaaaac atctgcatcg gaaaattt
//
LOCUS       DAJSBL010000121         4310 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.124, whole genome shotgun sequence.
ACCESSION   DAJSBL010000121 DAJSBL010000000
VERSION     DAJSBL010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4310
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.124"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(65..1285)
                     /locus_tag="ONT85_002810"
     CDS             complement(65..1285)
                     /locus_tag="ONT85_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HCR2379731.1"
                     /translation="MKLIGIVGSAAELSYNRKLLTFIQKQYHELFNLEIIEIKDLPLF
                     NQDDDQTNSPAIQQLNRKILMADGVIIATPEHNHTVPAGLKSALEWLSFKIHPLENKP
                     VMVVGASYYDQGTSRAQLHLRQILDAPGVNALVMPGNEFLLGKAKEAFDQEDRLIDPR
                     TVDFLGSTLASFVKFIKVVSTLAGPKKLPPEDLHATGTIATTIPDVDMSDDHWLEKAS
                     EAVGAVSGNTYVKLNRGLLTVDQINYFLDSMPMELTFADNNNQFLYYNLRQEAKEMLA
                     ARVPGQVGNPLANCHPEKSYKNVEWVIQQLRSGATDAVRVHVPKQVPEKYIVHNYQAM
                     HDENGEYAGINEYILDFKPIIEWYLQQTGQQLVGNEDAVSSASVHGGKTDAVTSASTH
                     DETAVDAVSSASVK"
     gene            complement(1305..1916)
                     /locus_tag="ONT85_002811"
     CDS             complement(1305..1916)
                     /locus_tag="ONT85_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HCR2379732.1"
                     /translation="MKMKFIGLVGTNSTRSTNRELLQFMKNYFVNEAEIEIVEIKELP
                     LFNKPENTELPNVVKEIAGKIEQADGVIISTPEYDHAVPASLINALNWLSYGIFPFVD
                     KPVMITGASYGTLGSSRAQAHLRQILDSPELKARIMPSSEFLLGHSLQAFDEQGRLID
                     EEQVAKLRELFKDFLLFVTITSQLQHAHQANKREAESFSWETI"
     gene            complement(1959..2891)
                     /locus_tag="ONT85_002812"
     CDS             complement(1959..2891)
                     /locus_tag="ONT85_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HCR2379733.1"
                     /translation="MNQVIEETKIWQMMGTTITLQVGHEEPSRLLAELGEWLHVYEHR
                     FSAHDATSELMAINQAAGRQAVIVHPELFELIKLGKAHSCARNSYLNIAIGPVVQKWH
                     IGFEDALVPTKAEITELLLLTDPHQIQLDEKMKTVFLTKPGMMIDLGALAKGYIADKL
                     ITHCRERNAAFALVNLGGNIVVFGESPRRSDGAWRIGLRNPKGSREESLAVVKLNNQS
                     LVTSGIYERYLQNGSSVYHHIIDPKTGFPVKNDVASLTILSDSSVDGEIWTTRLFGAS
                     SLEVLKALEEQPGIEGAVVTKENQFLYTKGFEKI"
     gene            complement(3312..4172)
                     /locus_tag="ONT85_002813"
     CDS             complement(3312..4172)
                     /locus_tag="ONT85_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR2379734.1"
                     /translation="MNIEKFITARKAANLSQKEVSEGICTQATLSKFENNGQVPSLKI
                     LIKLCNRINLPLVELFSSMNVINTQLDEQLEKAEFSLITSEYEQLTQILAAIDPKVID
                     ESELIARYYYLKGFEMIFTNAPITDVLFHFDQLLMAETANTLFRLLAYTGIGMVYARE
                     KELEKAEVYFNRVFEKIYTYPIRTTEDIWRVLNIVFHCGVFFAQDGDLETSDALLNYA
                     IDICSDNHLTYYLARAAAQLAKNAQAKNKSKAEVLELIQDALAFAKINKNKKLIDELN
                     HMKKMILLIN"
BASE COUNT         1230 a          866 c          707 g         1507 t
ORIGIN      
        1 agcttaaatc atagataccg taagggactt tattctttat ttaaaacttt gcaacagaac
       61 ctttttattt tacagaagca ctcgaaactg catctactgc agtttcatcg tgtgtgctgg
      121 cgctggttac tgcatctgtt tttccaccat gaacagatgc acttgaaacc gcatcttcat
      181 ttccaacaag ttgttgaccc gtttgttgta aataccactc aatgattggt ttgaaatcaa
      241 gaatgtattc attgattcca gcatactctc cattttcatc atgcattgct tggtaattat
      301 gcacaatata tttttctggc acttgttttg gtacatgaac acgtaccgca tctgttgctc
      361 cgcttcgcag ttgttgaatt acccactcta catttttata cgatttttct gggtgacagt
      421 tggctaatgg attccctacc tgaccgggca cacgagcagc taacatctct ttcgcttctt
      481 ggcgcaaatt ataatataaa aattggttat tattatctgc aaaagttaat tccatcggca
      541 tcgaatctaa aaagtaattg atttgatcca ctgtcaataa cccacgattt aatttgacgt
      601 aagtatttcc agaaacggca ccgactgctt cagaagcttt ttccaaccaa tgatcatctg
      661 acatatcaac atcgggtatt gtcgtagcaa ttgttcctgt cgcatgcaaa tcttctggtg
      721 gaagcttttt aggccctgct aaggtagaca cgactttaat gaattttaca aaacttgcta
      781 atgtactgcc taaaaagtca acagttcgtg gatcaatcaa gcgatcttct tggtcaaatg
      841 cttcttttgc ttttcccaat aaaaattcat ttccgggcat cactaatgca ttcacgcctg
      901 gtgcgtccaa aatttggcgc aagtgtaatt gcgcgcgtga agttccttga tcgtagtaag
      961 aagcacccac aaccattaca ggtttatttt caagtggatg gattttaaat gatagccatt
     1021 caagtgcact tttcaatcct gctggaaccg tatgattgtg ttctggcgta gcaataatta
     1081 ccccatcagc cattaagatt ttgcgattta gctgttgaat agcaggacta tttgtttgat
     1141 catcatcttg attgaacaga ggtaaatctt tgatttcaat gatttctaaa ttaaatagtt
     1201 catgatattg tttttggata aatgttaata atttacggtt atatgaaagt tcagctgctg
     1261 atccgacgat acctattaat ttcatctttg tttgctcccc tcgattaaat cgtttcccat
     1321 gaaaagcttt cggcttcacg tttatttgct tggtgcgcat gttgcaattg actagtgatt
     1381 gtgacaaaga gtaagaaatc tttgaatagt tcacgtaatt ttgcgacttg ttcttcatca
     1441 atgagtcgtc cttgctcatc aaaggcttgg agtgaatgac ccaataaaaa ttctgagctt
     1501 ggcataattc gtgcctttaa ctctggtgaa tccaatattt ggcgcaaatg tgcttgtgcg
     1561 cgtgaagaac ctaacgtgcc ataagacgct ccggtaatca tcactggttt atccacaaac
     1621 ggaaaaatac catatgaaag ccaatttaat gcgttaatca atgaagcagg aaccgcatgg
     1681 tcatactctg gtgtgctgat gattacgcca tctgcttgtt caatttttcc agcaatttct
     1741 ttaacaacat ttggtagttc tgtgttttca ggtttgttaa atagtggcag ctccttgatt
     1801 tcgacaattt cgatttctgc ctcatttaca aaataatttt tcataaactg taataattca
     1861 cggtttgttg accgcgttga atttgttcct actaatccaa taaatttcat cttcattctc
     1921 tttcctttct aacatcgatt tctgactgtt cgcacgtact aaattttttc aaatcctttt
     1981 gtatacaaga attgattctc ttttgttaca actgctccct cgatacccgg ctgttcttca
     2041 agcgctttca acacttctaa agaagatgct ccaaataaac gtgtagtcca gatttctcca
     2101 tctacagaag aatcagacaa aatcgtcagg cttgctacgt catttttaac tggaaagcca
     2161 gttttcggat caatgatgtg atgatacact gacgaaccat tttgtagata acgttcgtag
     2221 attccagagg taactaaaga ttggttgttt agtttaacca ccgcaagcga ctcttcacgt
     2281 gaacctttag gatttcgcaa accaatacgc caagcaccat ccgaacgtct tggtgattca
     2341 ccaaaaacaa cgatatttcc tcctaagtta actaaagcaa acgctgcatt tcgctctcgg
     2401 caatgtgtaa ttaatttatc tgcaatgtaa ccttttgcta acgcccccaa gtcaatcatc
     2461 attcctggtt tggttaaaaa aacggttttc attttttcat ctaattgaat ttggtgtgga
     2521 tccgttagca gaagtaactc agttatctct gcttttgttg gaaccaatgc atcttcaaat
     2581 ccaatgtgcc atttttgtac aaccggacca atagcaatat tcagataact atttcttgca
     2641 caactatgtg cttttcctaa tttgatgagc tcaaaaagtt caggatgtac aatcacagct
     2701 tgtcgtccag cagcttgatt gatggccatt aattcagaag ttgcatcatg tgcgctgaag
     2761 cgatgttcat aaacgtgcaa ccattcgcct aactctgcta gtaatcgact aggctcttca
     2821 tgcccaactt gtaatgtaat tgttgtcccc atcatttgcc agattttcgt ttcttcgatc
     2881 acctgattca ttttgtacct tctttacttg aactacggat tacatgagta attagttttt
     2941 atttttgtta ctgttttcac aaccagatga tacgcctgct agatttattt gtcaaccgat
     3001 tttatatatt tttttatata ataaggtaat tttaatataa aattttatat tgtaaaaaaa
     3061 caaataaggg aaacaaacaa cacatttttg tgtgttgctt atttccctct aactatcgtt
     3121 ttctatttga tggttaacct acttaaaata aatactgcaa cgacaaacgg agatcaagca
     3181 tgctttgtaa cgtattttac gataattacg actttatttt attactctat cttgctgcca
     3241 tacccgtcta tccctaatca tctttggtga attttttcaa agttagcata acaaaaataa
     3301 gtattttttc cttagttaat caatagtatc atttttttca tatgatttaa ttcatcaatt
     3361 aattttttgt ttttattaat tttagcaaaa gctagtgcat cttgaatcaa ttcgagcact
     3421 tctgccttcg acttattttt agcctgtgcg ttcttcgcta attgggcagc agctcgtgct
     3481 aagtaataag tcaaatggtt atccgaacaa atatcgattg cgtaatttag tagtgcatca
     3541 cttgtttcca aatcaccgtc ttgcgcaaaa aaaacaccac aatgaaagac gatatttaac
     3601 acccgccaga tatcttctgt tgtacgaatc ggatacgtat aaattttttc aaacactcga
     3661 ttgaaataaa cttctgcttt ttccaactct ttttctctag catagaccat tccaatacct
     3721 gtatatgcca ataaacgaaa caacgtgttg gctgtttctg ccatcaacaa ctgatcaaaa
     3781 tggaacaaca catccgtaat tggtgcattc gtaaaaatca tttcaaatcc ttttagatag
     3841 tagtaacgtg caatcagctc agactcgtca attaccttgg gatcaatggc tgctaaaatc
     3901 tgtgtcaatt gttcatattc actcgtaatc agtgaaaact ctgctttttc tagctgttca
     3961 tcaagttgtg tattgatcac gttcatcgaa gaaaaaagtt caactaacgg aagattgatt
     4021 cgattacata atttgattaa aatttttaac gaagggactt gaccattgtt ctcaaattta
     4081 cttaatgttg cttgtgtaca aattccttca gacacttctt tttgagataa gttggcagcc
     4141 tttcttgccg taataaattt ttcaatgttc attattttct ctccaatcag acctcacacg
     4201 aaaatcattt ctgtctcgat tttagcatgt tttctttggg aatgctttag gttctgttgc
     4261 aaagttttaa ataaagaata aagtccctta cggtatctat gatttaagct
//
LOCUS       DAJSBL010000122         4282 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.125, whole genome shotgun sequence.
ACCESSION   DAJSBL010000122 DAJSBL010000000
VERSION     DAJSBL010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4282
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.125"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..468
                     /locus_tag="ONT85_002814"
     CDS             <1..468
                     /locus_tag="ONT85_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HCR2379735.1"
                     /translation="FKEFHAIKNNSVLLIIGTGDMEEEIKNYAKKSGIDDDIKFLGNR
                     NDIEKFYQAFDVLMLPSLFEGVPLVGIEAQFADLPCFFSEKVPTEVAFSDKTNFISLN
                     QSAKEWAREIANVDISHRDSERSILIKADYNIQTAYKILESKYYELFELIQRG"
     gene            474..1496
                     /locus_tag="ONT85_002815"
     CDS             474..1496
                     /locus_tag="ONT85_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide synthesis protein"
                     /protein_id="HCR2379736.1"
                     /translation="MTQKHSSIKRIIHRISEELNATRRIAKITSWKEAFATLQAKIDI
                     QVMNRNGYNESKKQKEHLLKKHEVMLKYYEKTFSDFLKTYDYSQNQIINQSKSPYSNC
                     IWVCWWQGLDQSPEIVKACVKSIKRNAGKHTVIILTEDNYRQYVDIPKWVEEKKNKGI
                     ITRTNYSDLLRLSLLAKHGGLWLDATFYCTKPIPDEYFDIDIWSIRRPDYAHASVASG
                     YFAGYSLACNQESRWMFAIIRDFFLNYWKNNDMMVDYLMIDYMIVLAQRINHEIAQTF
                     DKIPSNNPLCDELIKNLNNIYDEKVWNLLKKDTLLFKLTWKQSFKKQKDDKVTFYGML
                     VENKLL"
     gene            1518..2573
                     /locus_tag="ONT85_002816"
     CDS             1518..2573
                     /locus_tag="ONT85_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HCR2379737.1"
                     /translation="MKHKVSVIVPIYNVEKYLRKCVDSLLSQTLKEIEIVLVDDGSPD
                     ASGEIADEYQKKYSNVKTIHRENGGLGPARNTGIENATGEYVAFLDSDDWVESDMYEK
                     LYLVASKENADIVVSGHCDFANDKAMVKKQHPLSGNVYDTPNSIKNVRKNLFGHSPKD
                     QEVEAFPMSVCMSLYNKNLLDKYRLRFEKILSEDTIFNLNAYDVANKIAFTEYTDYCY
                     RKEEQESITKSLSPNKQTEFKEFLITLKAIASKEDLECQIRAKRMAIDYCRLYVGLVD
                     DANLGIKEKRKYIKKFIEDEGLIQCWKGYPTNTLPFQQKFFHKMLECKCYTILLLLSR
                     IRKIIKNMTRKKLFIIL"
     gene            3191..>4282
                     /locus_tag="ONT85_002817"
     CDS             3191..>4282
                     /locus_tag="ONT85_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2379738.1"
                     /translation="MSVKKMLFMVSTIIVLTSRFWGINTSLSMRYFIMAIWDLYFMLS
                     YFSYREKTYKKYVIKNNFWLTIMPFVIFSIYTLIIWGIGSNAEFENFIKLCSTLLYLI
                     LAYGYACTAFYLFKAEGIDFIFWSGVISYMSGSVFYLIVSYGLNGLISYIKSLITGET
                     NTANFAMEVHDLTFAMGVFFLYYVFFENKNTKKHKIKIIVSLILIFMGLKRIELLALS
                     GAILVYYVLLKWGKKIQIRALVCTICATVISLVFIYIIDTGILALLMSNLGITDDGAR
                     LSFYLYSSKFYELGLGYVGTGWTWFSKYFFNLYLSHFRLNGGRIAASLHSDILTIYIE
                     VGCIVFIIWMFWLFFVKPIRLTKKFGLKTG"
BASE COUNT         1627 a          433 c          777 g         1445 t
ORIGIN      
        1 tttaaagaat ttcacgctat taagaataat tccgtattat taattattgg aactggtgat
       61 atggaagaag aaataaaaaa ttatgcaaag aaaagtggca tagatgatga tattaaattt
      121 ttaggaaata gaaatgatat agaaaaattt tatcaagcat ttgacgtatt aatgttgcca
      181 tcattatttg aaggggttcc gttagtgggt attgaagctc aatttgcaga tttaccgtgt
      241 ttcttttcgg agaaagttcc tacagaagtt gctttcagtg ataagaccaa tttcataagt
      301 ttaaatcaat cggcaaaaga atgggcgaga gagattgcaa atgtagatat ctctcatcga
      361 gattcagaac gttcgatttt aataaaggca gattataata ttcaaacagc atacaaaatc
      421 ttagaatcaa aatattatga gttatttgaa ttaatacaaa gaggttaaag aatatgacac
      481 agaaacattc aagtataaaa agaattatac atcgcatatc cgaagaattg aatgcaacaa
      541 gacgaattgc aaagataaca tcttggaagg aagcatttgc cacgttacaa gctaaaattg
      601 atattcaagt catgaatcgt aatggatata atgaaagtaa aaaacagaag gaacatttat
      661 taaaaaaaca tgaggtaatg ctgaagtatt atgaaaagac tttttcagat tttttgaaaa
      721 catatgatta tagtcaaaat caaataatta atcaatcaaa atcaccatat tctaactgca
      781 tttgggtttg ttggtggcag ggattggatc aatcgccgga aattgtaaaa gcttgtgtaa
      841 aatcaataaa acgaaatgca gggaaacata ctgttataat tttaacagaa gataattata
      901 gacaatatgt tgatatacca aaatgggtgg aagaaaagaa aaacaaagga atcattacga
      961 gaacaaatta ttctgattta ttaagattat ctttgctagc aaaacatgga ggtttgtggt
     1021 tagacgccac attttattgt acaaaaccaa taccagatga gtattttgat atcgatattt
     1081 ggtctattag gaggcctgat tatgctcacg catccgtagc atcaggatat tttgcgggtt
     1141 attcattagc atgtaatcaa gagtctagat ggatgtttgc tataattaga gacttttttt
     1201 taaattattg gaaaaataat gatatgatgg tggattattt gatgatagat tatatgattg
     1261 ttttagcaca gagaataaat catgaaatag cacagacatt tgataaaata cctagtaaca
     1321 atccattatg tgacgaattg attaaaaatt taaataatat atatgatgaa aaagtatgga
     1381 acttattaaa gaaagatact ttactgttta agttgacatg gaaacaatct tttaaaaagc
     1441 aaaaagatga taaagttact ttttatggga tgttggtaga aaacaaatta ttataaatat
     1501 tagaggtata aaaagttatg aaacacaagg taagtgtgat agtaccaatt tacaatgttg
     1561 aaaaatattt aagaaaatgt gtggatagtt tattgagtca gacattaaaa gagatagaaa
     1621 tcgtattagt tgatgatggt tcgccagatg cttcggggga aatagcagat gagtatcaaa
     1681 aaaaatattc taatgtaaaa acaatacata gagaaaatgg ggggttagga ccggcaagaa
     1741 atacaggaat tgagaatgcg acaggagaat atgtggcttt tcttgattca gatgattggg
     1801 tagaaagtga tatgtatgaa aaattatact tagttgcgtc caaagaaaat gctgatatag
     1861 tagttagtgg acattgtgat tttgcgaatg acaaagctat ggtgaaaaag caacatcctt
     1921 tgagtgggaa tgtatatgat acgcctaata gtattaaaaa tgtacgaaaa aatttatttg
     1981 gacatagtcc aaaagatcaa gaagtagagg ccttcccgat gtcagtatgt atgtctttgt
     2041 ataataaaaa tctgctagat aaatatagac taagatttga aaaaatttta tctgaagata
     2101 caatttttaa cttgaatgca tatgatgtcg caaataaaat tgcttttaca gaatatacag
     2161 attattgtta tagaaaagaa gaacaagagt ctattacaaa aagtttatct ccaaataagc
     2221 agacggaatt taaagaattt ttaataactc taaaagctat agcatcgaaa gaagatttag
     2281 aatgtcaaat cagagcaaaa cgtatggcaa ttgattattg taggctttat gtgggcttag
     2341 ttgatgatgc aaatttggga attaaagaga aaagaaagta tataaaaaaa tttattgaag
     2401 atgagggatt aattcaatgt tggaagggat atcctacaaa tacgctacca tttcaacaaa
     2461 aattttttca taaaatgctt gaatgtaagt gttatacaat attgttgttg ttaagtagaa
     2521 ttagaaagat aattaaaaat atgaccagaa aaaaattgtt tataatatta taaactaaaa
     2581 gtatatcaaa taatagaaat taacaagtta caaaaaaata ttataaagaa taatattttc
     2641 taaaaagtaa actatagtaa tgtggattat caccttccaa cagtatataa aatgagcgta
     2701 aaaacggcta tttttgcctt aacaacgatt tttttgtata caaatacgat atgctcatta
     2761 attattggaa tttgtagtgc tgtttaaatt aatgaaggtg taattatatg agtgatcatt
     2821 ttgaaaaagt ttgttgagaa gatgtgcttc tagtcatgtg atgggagaaa ataatttaac
     2881 tgttagagtg aatagttaaa ttattctggt tatatgaaaa aagtagtttc taaaataatc
     2941 ttgcagggat taacttaaag aaattgagag aatagtttct ttgaaatttc tatatacaat
     3001 gagaagctga aatttacttt tatttgatga ctggaaatag aaattaaata tattgtattg
     3061 ttacattaaa gaaaacttgt atggaaaaat cagtacaata ccattattta atgttcttca
     3121 cttaaagcgc aaaaaatatc agtttattaa actcgtatat agaacaaata tatgtagacg
     3181 gggcgaaacg atgagtgtaa aaaaaatgct atttatggta agtacaataa ttgtattaac
     3241 atcccgattt tggggaatta atactagttt atctatgcga tattttatta tggcaatatg
     3301 ggatttatat tttatgttaa gctatttttc ttatcgtgag aaaacatata aaaaatacgt
     3361 tatcaagaat aacttttggt tgactataat gccgtttgtg atattttcta tctatactct
     3421 aataatatgg ggaattggtt caaatgcaga atttgaaaat tttataaagt tatgttccac
     3481 gttgctttat ttaattcttg catatggtta cgcatgtaca gcattttact tatttaaggc
     3541 agaaggaatt gattttattt tttggtcagg tgttatttca tatatgagtg gttcagtttt
     3601 ttatttaatt gtatcttatg gtttaaatgg tttaatttct tatataaaaa gtcttataac
     3661 aggtgaaaca aatactgcaa attttgctat ggaggtacat gatttaactt ttgcgatggg
     3721 ggtttttttc ctttattacg tattttttga aaataaaaat acaaaaaaac ataagataaa
     3781 aattatcgta tctttaattt taattttcat gggattgaag agaattgaac tgttagcttt
     3841 aagtggtgca attctagtgt attatgtttt attaaagtgg ggaaaaaaga ttcaaatccg
     3901 tgcattagtg tgtactattt gtgcaactgt tatttcgtta gtgtttattt acattataga
     3961 tacaggaatt ttggcattgc ttatgtcaaa tttgggcatt acagatgacg gtgcgaggct
     4021 ttcattttat ctatattcat ctaaatttta tgaacttggt ttaggatatg taggaacagg
     4081 atggacatgg ttttcaaaat acttttttaa tctttattta tcgcacttta ggttaaatgg
     4141 tggaagaatt gcagcaagcc tacacagtga tatattaact atttatatag aagttggatg
     4201 tatagttttt attatatgga tgttttggtt attttttgtg aaaccgataa gactaactaa
     4261 aaaatttgga ttgaaaacag gg
//
LOCUS       DAJSBL010000123         4203 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.127, whole genome shotgun sequence.
ACCESSION   DAJSBL010000123 DAJSBL010000000
VERSION     DAJSBL010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4203
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.127"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(1..354)
                     /locus_tag="ONT85_002818"
                     /pseudo
     CDS             complement(1..354)
                     /locus_tag="ONT85_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(614..1162)
                     /locus_tag="ONT85_002819"
     CDS             complement(614..1162)
                     /locus_tag="ONT85_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2379739.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
     gene            complement(1163..2020)
                     /locus_tag="ONT85_002820"
     CDS             complement(1163..2020)
                     /locus_tag="ONT85_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HCR2379740.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYAEVE"
     gene            complement(2306..2592)
                     /locus_tag="ONT85_002821"
                     /pseudo
     CDS             complement(2306..2592)
                     /locus_tag="ONT85_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(2582..2911)
                     /locus_tag="ONT85_002822"
     CDS             complement(2582..2911)
                     /locus_tag="ONT85_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HCR2379741.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            complement(2988..3260)
                     /locus_tag="ONT85_002823"
     CDS             complement(2988..3260)
                     /locus_tag="ONT85_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HCR2379742.1"
                     /translation="MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQ
                     SFEKLKPTHEGRYSRRLNRQHRVVYKVDEDEHVVEIYSARTHYESQ"
     gene            complement(3260..3523)
                     /locus_tag="ONT85_002824"
     CDS             complement(3260..3523)
                     /locus_tag="ONT85_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HCR2379743.1"
                     /translation="MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIG
                     LDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVDEIDWDNL"
     gene            complement(3629..4045)
                     /locus_tag="ONT85_002825"
     CDS             complement(3629..4045)
                     /locus_tag="ONT85_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2379744.1"
                     /translation="MEFREGAFDFQTSQQNRKFIPEDFQNLQLENMRLEDLFRLADDD
                     KYRFEVLKIKKNELLNKLYPENAIKAHFGDANHMYSWFIPKRFRGKINAGDQVKVLNR
                     NSTDVVMITDIYVEDEVIIKYMKPVIKLVNKNKGNG"
     gene            complement(4051..>4203)
                     /locus_tag="ONT85_002826"
     CDS             complement(4051..>4203)
                     /locus_tag="ONT85_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HCR2379745.1"
                     /translation="NKFYQSLRTASSTIKGMETIRGIYKKNRRNGTLFSFSVSTEIKV
                     LMGITA"
BASE COUNT         1225 a          851 c          533 g         1594 t
ORIGIN      
        1 taaatgttct tactgcctct tccacactaa tttcttcgtt caatttcact aataatactt
       61 gatctcgctt attataatcg ctttcattta tcctaagagt caaatctttt tccaccataa
      121 cttttacacc caataaattt tgcggtttat ttttctctgt tgaaattctt tgtgtagcct
      181 ttaaactgac aattagctca ttgggtacat acttatttga gagtcatcta attggctcat
      241 aatcctttct ttttggttag tatctgcagt tacttgttta gtatgagcca tgaacccaaa
      301 catactcacc ccaacaacca atcctaaata tattttcttt ttaaactttt tcatctacat
      361 ctcctcctaa tagttttatt ttagagttag ctctattact tatttactaa ctcaacaaca
      421 gattatcata cacttgttaa tgaaatcaca atatctcatt aaaacacacc ctaaaatcat
      481 aaccgttcct atgctcattt tttggatttt ttaatcacgt aaccagaaaa ataaatcccc
      541 tctatttcac gcaaatcaaa gaagaactaa gtaactaccg tttaaaggta gttacttagt
      601 tcttctttga ttattatcta actttattat tcattcccca ccctttgcgc agtgagtctt
      661 cggtatcttg ttttatctga ctggtagttc ttattatttc tccacgatca tctaattctg
      721 gaacaattgg atatttttct ctgaacaagt ctagttcttt caccacgtca ttaaacaaat
      781 cttgcttatc ttcttgcgta agattctgtt taatatactc atctaaagct tttctaagca
      841 attcatctct tgtcatatta gtctcatact tttctactaa aactctggaa gtcgcattta
      901 acaaagtcga cagttttaaa ggatgcttgg tggaataaac cttatcaaac tttctgattg
      961 gtgcgccaac tttttttgtt cttcctctac tctgtctttc aacattggag gaagttttta
     1021 tttcttgaac ttggtgcact tctaattgct tattttcaga tggtgtaatt gcttgctgct
     1081 ttttctttgc ttctgcttct ttttttattt tggcgagtcc tttgttgttc ccaaaaatat
     1141 caagtccttt gatgtcagcc aattactcca cctccgcata ccaattctcg tgttcttcca
     1201 attctttcaa aatactgata aataattcgt ggcttttgcg atcccattgt tttaagagac
     1261 ctttctttgt cttctccatc gtaatacctg atccgtcgta tcgttttaag cgcgcatttt
     1321 tgagtacaac atttttaatc aaatgttccc cataaatctc ttgggcttgt ttatacattt
     1381 cctgatctac tgcgtcaccg ctttccaaca tgaaaggaat aacaccagca acatataaat
     1441 ttgtatcaaa ttctttcaca aaaaagtcca tatactcaag atattgcatc gcatttctca
     1501 tcgattttac ttgggtctgt aagacgacga ttgcccaatc cgcaatgtac atcgcggatt
     1561 tactatatac agaaatagaa ggtgggacat ctaaatagat gacgtcaaac tcatcaaata
     1621 atggttctag cttttctttc atatatttga attgggataa tgaatcatta tattggtctt
     1681 tgatccaatc atcatacatt tcaaaggaca tgtctgctgg aagtatatag aggttattga
     1741 ttactttata gacagcctct ttaggtggaa gattgtttat caacacgtca gtcacagtat
     1801 tttcgtaaac aatcggttca ccttctccgc ctccttcttc gttagtgaag ttcatatgcg
     1861 ttaagttcat cacatcagaa gtatttcctt gcatatccaa gtcaattatc aaggttcttt
     1921 ttccttcatt aactgctgat tcaaacccta acatttgcgc gcacgtactt ttacctacgc
     1981 cacctttaaa attaccgatt acatatttct tcgctaccac gttcctacct cttttctttt
     2041 agcaatattc atttgatact attataacat atttattttt agttactacc aaaaaaaaga
     2101 tatatatgta ctaccttata attaataagt aaaaggtagt acataaataa ggaaaagctg
     2161 aaatatattg atttatagct ttcctcaaag taatgacgtt atttaattta tcgagaagca
     2221 gagaatactc gcgactttag tcatgtagac gaatcagatt ttcgataaaa aagaagccgt
     2281 ccttcttaag aacgacaaca aatcactaga ttaatttttt caactgttca taaaagtttt
     2341 catgcgttcc acataacaat actggaataa ccttgttttc tataatctcg taagctactc
     2401 tatatgttgt gcccgcatat ttgaacccac tagaccaaat acctgataaa tccccagttt
     2461 tttgttcacc cttatatgga tcaatcgcaa tttcatcata aattaaattc aaaaattttt
     2521 ctttcaatgg cttctctttt agctttttaa atacttaatg acatgttttg aaggaacgat
     2581 ttcataagcc aatttctgtt gccaactcct ctttagtcat tactttagaa ttagtaagtg
     2641 tatcttcagc aatatctcta aaggaagaat gcattttgtt ttttctattc ttaaattcta
     2701 caagaatctc atctttatcg tagccttctg caattatatc agacaaaata tcttcgctaa
     2761 aatcataaaa ttctttttgt ggttcaacaa attcataaag aataccattc tctaccaact
     2821 ttgcttcaac ttcgactcca ttaggaacat taaaatcttt aggtactgtc aacatgattg
     2881 aattgccttg ttttcttgtt tttgtaatca tttaaatccc tcatttctaa cagtatgtaa
     2941 ttacttagta aatacattat accacgagat aatgtattta caagtaatta ctgtgactcg
     3001 taatgcgtcc ttgctgaata aatttcaacc acgtgttcat cttcatctac cttataaaca
     3061 acccggtgtt gtctattcaa tcttctagaa tacctacctt catgtgtagg ttttaacttc
     3121 tcaaatgatt gggtagggag atagggatct tcctttaaag tctgaataac cttctcaaat
     3181 tgaggtttca aattagactg tttgattttc tttaaatcag ctttagccga gtttttaatc
     3241 atcacagaat agttattcat tataagttat cccaatcaat ttcatcaaca ttagtaaatc
     3301 cactatcatc tttttctcgc ttacgaacaa cacccaaagt gcctatttgc tcaagtaata
     3361 gcgtttcttt gattgctttc caatcatcca aaccaataag caccgcatta ttttcactgc
     3421 gctcactaat gatttcaatt tccgtatgat tctcatttac atctttcaac aacttataaa
     3481 aatttttccg tgctgagcta ggatttaaga ctgtttttgc catatctact ccccctctaa
     3541 tttatatctc cattctaacg tacttaatta agtacgtaaa ttcgattgtt ttgtttaaat
     3601 tggagtacca aaacagcatt gtaaagtgtt atccgtttcc tttattttta ttaacaagtt
     3661 ttatcacagg cttcatatat ttgattataa cttcatcttc tacgtagata tctgttatca
     3721 ttacgacatc tgtactattt cgattgagta ctttcacttg atctccagca ttgattttcc
     3781 ctctaaatct tttaggaatg aaccaactat acatatgatt agcatcacca aaatgtgctt
     3841 ttatcgcatt ctcaggatat aacttattca atagttcatt ctttttaatt ttcaaaactt
     3901 caaatctata cttatcatca tctgctaatc taaataaatc ttctaaacgc atgttttcta
     3961 actgtaagtt ttgaaaatct tccggtataa attttcggtt ctgttgcgaa gtttgaaaat
     4021 caaacgcgcc ctctctgaac tccaaatatc ttaggctgtt attcccatta ataccttgat
     4081 ttcagtagac accgaaaagc tgaagagcgt tccatttctt cggttctttt tatatattcc
     4141 tcgaatggtc tccatgccct taatcgtgga agaggctgta cggagacttt gataaaattt
     4201 att
//
LOCUS       DAJSBL010000124         4176 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.128, whole genome shotgun sequence.
ACCESSION   DAJSBL010000124 DAJSBL010000000
VERSION     DAJSBL010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4176
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.128"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(124..582)
                     /locus_tag="ONT85_002827"
     CDS             complement(124..582)
                     /locus_tag="ONT85_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCR2379746.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(595..1926)
                     /locus_tag="ONT85_002828"
     CDS             complement(595..1926)
                     /locus_tag="ONT85_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HCR2379747.1"
                     /translation="MMMKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDE
                     ERNMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKI
                     PLKHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVR
                     RKYPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREI
                     MPEIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVV
                     EHTDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLND
                     DRRRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAV
                     EKLLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(1937..3379)
                     /locus_tag="ONT85_002829"
     CDS             complement(1937..3379)
                     /locus_tag="ONT85_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCR2379748.1"
                     /translation="MRIFNRGGIKMNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADD
                     MLNIPVIGAAGNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMD
                     PEVNMGIFGGIISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLG
                     FIWPLIQIGIDNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSP
                     SKEVFRGEMTRFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMG
                     SGAGTSFIANITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVD
                     YLINFRIATNAILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEET
                     ELGLASKNYEYMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISG
                     IIKPTEKNYQIIIGLEVTYVMAEFNKLLEE"
     gene            complement(3366..4130)
                     /locus_tag="ONT85_002830"
     CDS             complement(3366..4130)
                     /locus_tag="ONT85_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HCR2379749.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
BASE COUNT         1276 a          895 c          609 g         1396 t
ORIGIN      
        1 aaaaaaattc gataattaac aaaaatcaaa atgattaaat ctagtgattg aaacatatca
       61 tttcaaaaaa attttgtaaa ttcacccata aatatcaccc tatttaactt ttttcatcgt
      121 acactaaaat tcaaaacaac tatctttaat ctttacttct tggttttgtt ttaataaatt
      181 tccttcaaaa atgcctggag aaactacaat gatcaccgta tctttttgtg cttgctccaa
      241 cttcttcaaa tctataatag ctaacagtgt cccttctttt acctcctggt tactttcaac
      301 ccgtatctga aacggacttc cttcgagttc aactgtatct aaacccatat ggatcagtat
      361 ttttctgcca gattcacttt cgatcgttat ggcatgtcgg gtaggaaata tattatcgat
      421 tattccttta actggcgcat ggatatatcc atcatgattc gttatagcgt atccaactcc
      481 tatcattttt gaagaaaaga cctcgtcttt tactgctgtt aatggaataa tttgaccact
      541 agctacagct tttagaatgt ttcttgtttt aaagaaatgc actttttatc acctctactt
      601 taatgctggc caatattttt tgtttacttt gatcatttca tctagtattt tttttgcgac
      661 tctggcattt ggtacggttt gattcaatgt aaatgcttgt aaagcttttt gataagaacc
      721 ttcaaagtaa gcatctacta gtaacttctc tactgcaact tgtgcctcca tcaatccttt
      781 atgaaaatca ctgattggtt ttcttagtgt aattggctcc acaccagttg caccaacata
      841 tgccggtatt tcaacaactg catctgaccg tagattcgga atcgcccctt gattaggaac
      901 gattagcata aagcgacgac gatcatcatt caacaaggaa atagctatat cgacgatata
      961 ttgtccatgc tccccaaaat taaagttcaa tacttcttcc acaataccgc ggcgtatttt
     1021 atcagccatt tctttggtat ttttctctcg tccatccatg accatattag cacgtgtata
     1081 ctctttgtca gtatgctcaa cgaccatatc aggatataag taatactcta agtaattatt
     1141 cgggatatta cttggaaaat ttttcgtgat gactgacatc atttgaaagg ttttttgcca
     1201 agttttatct cctatattaa aatcggcgac ttgcatttct tttgttatta gtttttcaat
     1261 aatctctggc ataatttctc gttgcaattc tttatcataa atcgatgtat accagccaaa
     1321 gtggtttaaa ccataatacg taacgatcca attcttacga tcataaccgt aattcactgc
     1381 aatcgtttct tcaattgaaa tagtcatatc acaggcatta atcattttta cattcggata
     1441 tttacgacga acagcttcag aaacgatcga ctctggattt gtataattca aaatccatgc
     1501 tttgggggca tagtcttgta tatgatcgac aatttccaat aaacctttga ttgagcgcat
     1561 cccataagca aatcctccga gtccacacgt ttcttgtcct actaatccat gtttaagtgg
     1621 aatcttttcg tccttttctc gcatctccaa tcctccaaca cgaatttgtg agaaaacaaa
     1681 atcgcatcct gtaaaagcaa gctttggatc atccgttgca cgaatgcgaa tatttgaaaa
     1741 accttttctt ttcagcataa atttaacaat caagtacata tccatatttc tttcttcatc
     1801 aatatcatat aaacgtattt cagataatgg caaccgtttt tcattgttca aaatggcttg
     1861 aacgattcct ggtgtatacg tactcccgcc tccagctata gtgataatct gttttttcat
     1921 catcatcttt tcctttctat tcttctaata atttattgaa ttctgccata acataagtca
     1981 cttctaagcc gatgataatt tgataatttt tttcagttgg tttaatgatc ccgcttatcc
     2041 ctaattgctt gattcgctgt tcctcgacca tggtgggatc cactacctct acccgtaggc
     2101 gtgtattaca actatagaca tccaggatat tggcttttcc tccaaacgct tgtagcattt
     2161 ttgttgccat atattcataa tttttacttg ctaaccctaa ctctgtttct tctaaggtta
     2221 cttcattgcc aaactctgcc ttagcttcac gtcctaatgt ctgaatgttt ctcttattga
     2281 tcaaataata aaaagtaacg taatacaagg caaaaaagaa aattccgatt ggaagaatca
     2341 aaatagcatt ggtagcgatt cgaaaattga tgagataatc aacaatacat gctccgaatg
     2401 taaaaccaat tcgtatattg aataagtaac agacaatccc tgctaaaccg gcaaaaagtg
     2461 catggatcac gtacaacata ggagaaagaa acatgaaact aaattcaaca ggctcagtga
     2521 tatttgcaat aaatgaagtt cctgcaccgc ttcccatgat tcctttgacc atctctttat
     2581 tttctttata agctgcttta tagatagcta gacatatagc tggtatacca aacatcatta
     2641 ctacaaaaaa aactgataaa aacagtccag cctgaggatc tccgttgata aagcgtgtca
     2701 tctctcctct gaaaacttcc ttgctaggag atgtatagct tcctaaatcg taataaatat
     2761 acgtattcaa cacatggtgc agtccaaagg ggatcagcaa acgattcaaa aacataaata
     2821 tccccacacc taatgctccc atatcaacga gcagttttgc aaaattatca atcccaattt
     2881 ggatgagcgg ccagataaag cccaaaaaaa cagctgcgat tgtttgtaga atcatcacca
     2941 tagtcaatgg gaatttttcg cccgcaaaat aagaaaaaac tactggtaat ttttgttctt
     3001 taaagcgatt atacgtccat gccgctacta aaccggaaat aattccacca aaaatcccca
     3061 tattcacttc tgggtccata atttctaagc cttttttcaa ggtagcaatc gctaaaaaac
     3121 cagtaagtgc aggtattcct ttgtccttat ttttgacaaa accgattgca actcctatag
     3181 caaataagat atccaagttt ccaaaaacgg catttccagc tgcaccaatt actgggatgt
     3241 tcagcatatc gtcagctgat aaacgcatga tcaatcccgc taatggcatc gctgcaactg
     3301 gaagtagcat cgctcttcca agcttctgca catttgattt aacattcatt tttattcctc
     3361 ctctattaaa tatcctcata tgttactaac tcaatcaaag gatcattttt cactttttga
     3421 gcaaatgctg aatgaattaa aaattcgact tggtgggtac gaggatacac aaaacttgaa
     3481 ccatttaaca attcctgatc caaatacgct gcgtgacaca tgacttcgat cgttttatct
     3541 tctctcaata tcatgagtaa acttgccaaa tattcttcca cactcgtatt gatttgtcgt
     3601 tttttttcat cagtttctcc tacgacatcg aaacaaggct caaaataatc gacatgacga
     3661 acttcatctt ttcgttcaaa gttgctccga accggcaaat catatttttt tgctaatgta
     3721 acaactacct cctgattttc atccaaagta tgagtatgat gatgactgtc taaatgagtt
     3781 ggtatgatcc ccgcatgata aattttttga atctgagcat tccattcctg atacagttga
     3841 tctttgtcaa tgttaaatgg atgctgataa taagataagg atctgaactc tccgctttcg
     3901 ataagagtat cgacagtttt gaggagtggt ttgccacacg tcaatgttaa atggactcct
     3961 acacctagcc taggcatttc ctttcttagt ttcacagcat gctcgaaacc cggcatattt
     4021 gccatcaatg tagttgatgt caaaattcct cgctgataag catccataat cccataattc
     4081 acaccatggc tatagccaaa atcatccgaa ttaaaaatca cttttcccat ttcattctcc
     4141 ttcttttttc tactaaaata ggcgcaacca aataat
//
LOCUS       DAJSBL010000125         4036 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.129, whole genome shotgun sequence.
ACCESSION   DAJSBL010000125 DAJSBL010000000
VERSION     DAJSBL010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4036
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.129"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(242..391)
                     /locus_tag="ONT85_002831"
     CDS             complement(242..391)
                     /locus_tag="ONT85_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2379750.1"
                     /translation="MTFTKTNMQYRVAFDTTSNRFMAIDAQNENNVAFGITIEQAVRA
                     LNESR"
     gene            853..1065
                     /locus_tag="ONT85_002832"
     CDS             853..1065
                     /locus_tag="ONT85_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2379751.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPAELVS"
     gene            complement(1343..3142)
                     /locus_tag="ONT85_002833"
     CDS             complement(1343..3142)
                     /locus_tag="ONT85_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HCR2379752.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
     gene            complement(3266..3862)
                     /locus_tag="ONT85_002834"
     CDS             complement(3266..3862)
                     /locus_tag="ONT85_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HCR2379753.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
BASE COUNT         1449 a          711 c          689 g         1187 t
ORIGIN      
        1 atattaaata ttatacagat aataacggaa tattatcatc gaaacagcta acaaaattgt
       61 cataaaaaac gagatggttg atattagaac ttgtgttttt gtttaggtct agagataaag
      121 agagacaatt tccaaatata tgtaatgaat acaaaaagac gtttgtcgcg gattaccgtt
      181 acaaacgtct tttattgatt ttccgagatc tcatcagtcg ccatttggaa tggttcactc
      241 cttatctaga ttcgtttaat gctcggactg cttgttcgat tgtaatacca aacgcaacgt
      301 tattttcgtt ttgtgcatca atcgccataa aacggttaga agttgtatca aaagctacgc
      361 ggtattgcat atttgttttt gtaaacgtca taaaattcat ctcctttgta atgtaattta
      421 gttcttgaaa gtttccttta aactttcgaa cttataccaa gtttagcatc gtacgttttt
      481 tgatgcaatt aaaaagcatt tatcattttc cttatttatt ttttcttaaa tggtttagct
      541 attttctttt aatccgataa gtagcccaat catcaaggtt acaaatacta ttgcttgaat
      601 ataaatcata tgctcacctc tttataaata acttagcaat ataaagaaaa aaacgcacga
      661 acaacatagg aatataaaca aacgtttttg tcacaacgtc taagatcgtg ttaaaaaata
      721 gttatctaaa aagcagaaag atactcaaaa taaaaaataa aaaaatggat aaaaacgttt
      781 tcaaatacaa ggtaatttgt tattataaaa taaagaaaaa acaaaataaa aataaagaaa
      841 agagggaatg aaatggtaaa agcagaagta ggaagatctt atatatgcaa accgatcgga
      901 gtatctcatg aactaatcgg ctgtattgaa cgtacatata tcaacacggc tgtcgtgtat
      961 gttgaaagtt atgaccaacg agactatcaa cttatcaatg aaagcaagta tcgcatgctc
     1021 gtaaaattct gtgatattag cgcacctgct gaattggttt cttaaacagt atttttgaga
     1081 attcgcaaga actcccttat aatataaata aagacagaag ggagcgagaa gatatgccaa
     1141 aaagaaaaag ttcagggaaa atgggaatta ttcttgcagt gatctctgca attcctgcta
     1201 ttttagctat tgttcgtgcg atgaaggaat caagagaaag ttaagcacac attatgagcg
     1261 tcaaacaaaa aacgcatata cctgcggtgg caggttatgc gttttttgtt gtataaagac
     1321 ttttttcatt aacgattttt gattagccga ctcccaaaac aaaatttagc agtttatctg
     1381 aataagtgat ttcttcatct gtttgtttca cactttcatt tagtagtccc atctcatcgg
     1441 ttatgatacc atccacacca taaaacatca tgcgactcat agcatctgaa tcgttgattg
     1501 tccatacata tacttctttt ccgtcttggt gggcagaatt aatgaagtta cggttgatcg
     1561 tactatattc cattgtcaga aaatcagcag gcgtcacagg gggcccaaca atattgaaag
     1621 gtaagatgta gcccacataa aagtttggtt ctgcttcttt taaatctgaa acggtttggt
     1681 acgtcaaact ttgcaaaata tgatggttct ctaagataac tttgcggtat ttatggacaa
     1741 atcgttcgac aaggtcatcc gaatcttttt tagaggtctt gatctcaata agtaattttt
     1801 ggtttagctt atgtgctttt tccaaataag aatcaaaaga acaaacaggg gcttgcactc
     1861 cgttttcagt aactgtaaga tttgtcaatt ctttcaatgt aagatcctgt ggtgttccat
     1921 ttacccctgt taagtgacga agatttgcat catgcatcac tacgaaatga ccatcttttg
     1981 tttcctgaac atccatctct acataatcag gatgatagtc tctgcttgtt ttttcaagtg
     2041 cttctaaagt attttgcgcc ccattttggg tactcacacc tctgtgagag acagtcactg
     2101 gttttgtctg aacagctgaa gtcagataat ccatattgta aagacagacg ccaataccga
     2161 aaaaaactgc aaaaagagtc aaaccagtgt ttttcagcgc agagaaacgc aagttggggg
     2221 cttgtgggat aaaccactta gggatctcag gcaagaagcc ttcgtcatcc atgaaatcaa
     2281 caatgatata aaaaatccct acggtagaca tcacgatatt gaataataaa atcccttgta
     2341 aaagcgtcat agcaaacaca gcgctgatca atgcatagtc tgggaaaaat tgttcaacca
     2401 tactttgtgc caaaataaca acgatatatc ctaaagaaga aaggagtaaa atcgtaccgc
     2461 caatcacaat aaattggccc gtgatggcca aaagacgcga tttggtcaat gaccagcttt
     2521 cccgaatggc tgctcgaaaa ggacgatctc gtagaatcat ttccggtaaa gcaaaaatca
     2581 gtcgaatccc aatatagcca aggaaaatat aaacgagtaa aaaggaactg acgatgatcc
     2641 aacgattagc aaaaataaaa tccataatga aagcggggat ttttattttt gacagtagat
     2701 cagaattaaa acttaatccg ctgatcggta gaatcaataa aaaataagca aggaaaaaca
     2761 aaaaagtaat gggtctcact ttcttcagct gaagtatcgt caagtgtaag agttgcttca
     2821 aagagattgg ttctttcttt ttaataaaaa aaacactcat caaaagaaaa gtaaattcga
     2881 aataaactaa cagtaaaatc gtcatcaaaa ctacgattaa cgagagtaga accccaggat
     2941 gttgagaaaa aattgaagga atattgtcat aagacaaata gtcaattgcc cctcggtgta
     3001 aaataaaacg ggtcgtactg ctcaataagg gtaaacaaat aaataaaata aagccatgca
     3061 tcagcaagac atcacggaaa taagcttgtg ttccttttaa aaagtcccaa gcattaccta
     3121 agctacgttt caaataattc atcctatcac ctgctcttca cattgtagcg caaatctaat
     3181 gaaaaaggaa agggtgcagt gtgttttgca gaccctttcc cagatttaac attgtcaatc
     3241 aatgactaat tttacaaaat aaatattatc caagaagacc acttagatat tgacctccat
     3301 aaatcaaggc catactatat aagaaaatcg acagtggttt agcaatcaaa atgattagtg
     3361 tatatcgttt cactgacatg tttgtcaatc cagccatcaa acataacgca tcgtcaggag
     3421 caactggaag aaaaatagct agagcaaaca gcttctcaaa acggttttga ttatccagcc
     3481 agccaatata tttgttatat gtcttatcgc taatcatgga aagtatgaat ggctttccat
     3541 aacgacgccc tagcagaaaa atgataatcg aaccaatgca gattccaata tagttataga
     3601 taaatccagc ataagggcca aatatcagga caccagctgc agtactgatc cctccaggaa
     3661 tgattggaat aactacttgc aggatttgga tgaagacaaa aataatcggt cctaaaatga
     3721 tggaatcacc tactaatcct cttaacgcat tcaaatcttt gaaaacacct aaattaataa
     3781 aataaatcgt cagcgcgatt gataggccta atccgataat ggatatgaaa tttatcagtt
     3841 ttcgagaggc agcaatattc attattcatc aactccttag aggttctttg ttaactatac
     3901 tttactagaa aagcaactaa ctgcgccatt ttttgatatg acagaaaaca aacaattttg
     3961 taagttcatc ataagtataa aagattgatc tcaaaaaaac atccaactaa agacttattt
     4021 aataacttgt tttttt
//
LOCUS       DAJSBL010000126         3987 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.130, whole genome shotgun sequence.
ACCESSION   DAJSBL010000126 DAJSBL010000000
VERSION     DAJSBL010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3987
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.130"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..938)
                     /locus_tag="ONT85_002835"
     CDS             complement(<1..938)
                     /locus_tag="ONT85_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="HCR2379754.1"
                     /translation="MGFSISFKKGTKNTSISHNNRELTDRQKTNDWHKHIDFTKSDEN
                     IYLEQTNLADKYEELFGEVVEKYNAKQKRADRKIEDYLAKVRKDKKLEPQREFIVQIG
                     ILDDFQTNRADGSSTGISKQQAEQNRVIANKILVQYYKEFQERNPNLAVYNAVIHNDE
                     ISPHLHLNIVPVAEGYKRGVQKQPSFNKALVQQGIHADKDNGRVLFNNFRNQEVDSIE
                     RLMNDYGWKRDIVGTNKIKDIHEYKEIMSEISELRQTAVKERESVSDELRYIYRQREE
                     NERDKALMASERAKIEEEKKNMLNVKKEMAVIFQKD"
     gene            complement(1160..1522)
                     /locus_tag="ONT85_002836"
     CDS             complement(1160..1522)
                     /locus_tag="ONT85_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2379755.1"
                     /translation="MGMTLADIKAQEEKLKKEQAKLRQQKKRLIEKENAAMGKALRKR
                     FGFETFADFERWLSSISDDQQHEKTVDNEWLADEKSAPSERDYPDESDDLFGVIPNKK
                     SQMSESKFTSIFNDVNLR"
     gene            complement(1526..1864)
                     /locus_tag="ONT85_002837"
     CDS             complement(1526..1864)
                     /locus_tag="ONT85_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2379756.1"
                     /translation="MVAILEVRCDMDKIDLREETVDRIELMYERLADYLNLPDEDNYS
                     EMLERIMDVLNSVINADLAKSSVLTNEFVHKHLAQTTDESEKEFWLKLMSHVMQLNQI
                     SVINSKEKEM"
     gene            complement(1843..2262)
                     /locus_tag="ONT85_002838"
     CDS             complement(1843..2262)
                     /locus_tag="ONT85_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2379757.1"
                     /translation="MTDLPEKMTIKNLADELGISKNTVRNQLVNAGYDLARYKDNGVI
                     MLDKELIGVVRSIYRSKGGQLTGDLPVNDAGNDFPLEKENEFLREQLAEKDRHISELT
                     EMIRDQQKLTLLQQQKNDQLLLENQELKNRKWWQFWK"
     gene            complement(2255..2488)
                     /locus_tag="ONT85_002839"
     CDS             complement(2255..2488)
                     /locus_tag="ONT85_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2379758.1"
                     /translation="MAEKQKHFGKKNWLVNLTGDNVVVVFLDSKKMSGKLLDVTQYEL
                     ILKNNKIDSPIIIPKHAVKYVYGVAESNGEDND"
     gene            complement(2491..3459)
                     /locus_tag="ONT85_002840"
     CDS             complement(2491..3459)
                     /locus_tag="ONT85_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HCR2379759.1"
                     /translation="MSKKRTIVNEWEKLTALSNRRLNVQTLVQTIKLNWSIDRITIVG
                     KLNQNIFYHTQHDVLILDFEQLMRVNEGNGYLKAVGNNGWQLLDQHDENIAYIEISKW
                     QEGKGRIDFNPNKISRFLADSMKNFIHDLFLEPHFSRADVACDLIDVPDEFITQYRII
                     DPVSFKLIYGRSGKLETVYWGSRASERQIRLYNKKIEQEMKRKVVPSEINTWWRLEMQ
                     LRRGKATEWYAMVKESLDSFVSPHFLPTDIKPTDKVMIDGLLANHSNWAILSRNSKYK
                     YRNLLKRESQNDELTNHLRETFEESADDLKKELDTWLQGLDVTEEE"
BASE COUNT         1131 a          892 c          549 g         1415 t
ORIGIN      
        1 atatcttttt ggaaaatgac cgccatctct ttttttacat tcagcatatt tttcttctct
       61 tcttcaatct tcgctctctc agaagccatc agagccttat cacgctcgtt ttcttctctt
      121 tgcctataaa tatacctcaa ctcatcagaa acgctctcac gctcttttac agccgtttgg
      181 cgcaattcag aaatttcact catgatttct ttgtattcgt gaatatcttt aattttgttg
      241 gtaccgacaa tatccctttt ccaaccgtaa tcattcatta atcgttctat agagtcaact
      301 tcttgatttc taaaattatt gaaaagcact cgaccattat ctttatcagc gtgtatccct
      361 tgttgaacca gtgctttatt aaagctcggc tgcttttgaa caccccgttt ataaccttca
      421 gcaaccggca caatattcaa gtgcaggtgc ggacttattt cgtcattatg aataacagca
      481 ttataaacag ccaaattagg gtttctctct tgaaactcct tgtaatactg aaccaaaatt
      541 ttattggcaa tcactcgatt ttgttcagct tgttgtttcg atatgccagt agaagaacca
      601 tctgctcgat ttgtctggaa atcatctaaa atgcctattt gcacaataaa ttctcgttga
      661 ggttccaact ttttatcctt gcgcactttc gctaaatagt cctcgatttt tctatcggct
      721 cttttttgtt tcgcattata tttttctacc acttcgccaa acaattcctc atatttatct
      781 gctagattcg tctgctctaa ataaatattt tcatccgatt tcgtgaagtc aatgtgctta
      841 tgccaatcat tggttttctg gcgatcagtt aactcacgat tattatgaga aatacttgta
      901 ttcttcgttc cttttttaaa tgaaatacta aaccccaagt taaaaccctc cttcgcgctg
      961 tcgctaataa aaagattgta aacctttctg ttactctttg tcaaagagta acattttcct
     1021 aattgtgaca tggatgtcac aattccaaac gctctccgag ggttttcgct gtcacaagtg
     1081 acaacgaaaa aaggagccga aagcgtatca cgctatcaac tccttttatg ctttattttg
     1141 cttcacgcaa aataatcgct tatcgtaaat tcacgtcatt aaatattgac gtaaatttac
     1201 tctcactcat ctgacttttc ttattcggta tcactccgaa taagtcgtca gattcatcgg
     1261 gataatctcg ttcgcttggc gctgattttt catcagcaag ccactcatta tccactgttt
     1321 tttcgtgttg ctgatcgtca gaaattgacg ataaccaccg ctcaaaatca gcaaaagttt
     1381 caaacccaaa acgcttgcga agagccttgc ccattgcagc attttctttc tcaatcaagc
     1441 gttttttctg ttggcgaagt ttcgcttgtt ctttttttaa tttttcttct tgcgctttaa
     1501 tatctgctaa agtcattccc ataaactaca tctccttttc tttggaatta ataacggaaa
     1561 tttggtttaa ctgcataaca tgactcatta atttcaacca aaattctttt tctgattcat
     1621 cagttgtttg cgctaaatgc ttatgaacaa attcattagt caaaactgaa gattttgcta
     1681 gatcagcatt gataaccgag ttgagaacat ccataattcg ttcaagcatt tcactataat
     1741 tatcttcatc cggcaaattc aaataatcag ctaaccgttc atacatcaat tcaattctat
     1801 cgactgtttc ttctctcaaa tcaattttat ccatgtcaca cctcacttcc agaattgcca
     1861 ccattttcga ttttttaatt cttgattttc tagtaacaac tgatcgtttt tttgttgctg
     1921 aagcaaagtc aatttttgtt ggtcacgaat catttccgtt aattcagaaa tatgcctatc
     1981 tttttccgct aattgttcac gcaaaaattc attttctttt tctaacggga aatcattgcc
     2041 tgcatcattg accggtaaat caccagtcaa ttgaccacct tttgaccgat aaattgaccg
     2101 cacaacacca atcaattcct tgtctaacat gataacccca ttatctttat accgagcaag
     2161 gtcataacct gcattgacca gttgattgcg cactgtattt ttagatatac ccaactcatc
     2221 agcaaggttt tttatggtca ttttttctgg caaatcagtc attatcttct ccgttacttt
     2281 cagctacccc ataaacatat ttaacagcat gcttagggat aatgattggg gaatcaattt
     2341 tattattttt caaaatcaac tcatactgcg ttacatcaag taacttaccg gacatttttt
     2401 tgctatccaa aaaaacaacg acaacgttat cacctgttaa attcaccaac caatttttct
     2461 taccaaaatg cttttgtttt tctgccatta ctattcctcc tctgtcacat caagtccctg
     2521 taaccaagta tccaattctt tttttaaatc atctgctgat tcttcaaatg tttctcgtaa
     2581 atgattcgtc aactcatcat tttgactttc tcgctttaat aaatttcgat atttatattt
     2641 cgagtttctt gacaaaatcg cccaattaga atgattagct aacaatccat caatcataac
     2701 tttatccgta ggttttatat cagttggtaa aaaatgtgga ctcacaaagc tatccaaact
     2761 ttctttcacc atagcatacc attccgtagc tttaccacgt cttaactgca tttctaaccg
     2821 ccaccaagta ttaatttctg aaggcaccac ctttcttttc atttcttgct ctattttttt
     2881 attgtacaaa cgaatttgcc gctcgcttgc ccgactaccc caatacaccg tttccagttt
     2941 tcctgagcgt ccataaatta atttaaatga aactggatca ataattcgat attgcgtgat
     3001 aaactcatca ggcacatcaa taagatcgca ggcaacatcc gctcgtgaaa aatggggctc
     3061 taaaaataaa tcatgaataa aatttttcat ggaatccgct aaaaatcgac tgattttatt
     3121 aggattaaaa tcaatccgcc cttttccttc ttgccattta gaaatttcaa tataggcaat
     3181 attttcgtca tgttggtcta atagctgcca accattattt ccaaccgctt ttaaatagcc
     3241 attgccttca tttacccgca taagctgttc aaaatctaat attaatacat catgttgagt
     3301 atgataaaaa atattttgat tcaactttcc aacaatggta attcggtcaa tcgaccaatt
     3361 taattttata gtttgtacca aagtttgaac atttaaccgc ctgttagaca gggctgttaa
     3421 tttttcccac tcattcacga ttgttctttt tttactcata ttcagttcct cctcaaagaa
     3481 aaaagagatt tttggaaaga atttccagag ttcacgtaac tctaaaaatt cataaccatc
     3541 aatctctagt cttgaagaaa cctaaaaata ttcgtataat gaatttgagt tacggaacta
     3601 gtgtggttag tttccgtaaa ggtcgagaac gttggtagcg ttgctcgact tatttttttg
     3661 ctctttttcc agttcatttg tttgctaact caataatagc acttctcttt agctatgcaa
     3721 ataccaaaaa aataaaaccg caccctttga aaagggcgcc cccaaacttc gctaacgcta
     3781 agttggccaa aaaatggact tgccattttt tagcaaatgt cagcttcagg ggctagtggt
     3841 ttagccgtca actccttatc ctcggcacaa accgcctccg gtaccgttga ctgcacatcg
     3901 tggctaccac tcaatctcat tctcagaacg ctctaattta tgctgtgaga cgttttgacg
     3961 ataaaataag gttttctcct gctctaa
//
LOCUS       DAJSBL010000127         3714 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.131, whole genome shotgun sequence.
ACCESSION   DAJSBL010000127 DAJSBL010000000
VERSION     DAJSBL010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3714
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.131"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(162..2159)
                     /gene="tkt"
                     /locus_tag="ONT85_002841"
     CDS             complement(162..2159)
                     /gene="tkt"
                     /locus_tag="ONT85_002841"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HCR2379760.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2261..2512)
                     /locus_tag="ONT85_002842"
     CDS             complement(2261..2512)
                     /locus_tag="ONT85_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HCR2379761.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2659..3282
                     /gene="lexA"
                     /locus_tag="ONT85_002843"
     CDS             2659..3282
                     /gene="lexA"
                     /locus_tag="ONT85_002843"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HCR2379762.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3462..3680
                     /locus_tag="ONT85_002844"
     CDS             3462..3680
                     /locus_tag="ONT85_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2379763.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
BASE COUNT         1026 a          797 c          688 g         1203 t
ORIGIN      
        1 ctcaagaaac acaaactaat ccgaagaatt ctttgttttg tcatttaaat gtaacatagc
       61 cgaaaacgaa aagtaatatt gatttattct tctaacacat aaagaacagc tgtctccaac
      121 ttgcagacag ctgttcttat cagtcaaaca attgcttgga tctatgatag ttgattgtac
      181 gtattcacga cattttctac tgtaaatcca aattcttcta aaattttagt gccaggggca
      241 gatgcaccga aatgatcgat tgtaatcgtt gcgccctctg ttcctacgta gcgttcccat
      301 ccaaatgatg cagcagcttc gatagccaca cgtttcttca ctgatttagg aagtacactt
      361 tctttatatt cagaagattg tttttcaaag aggtcaaagc ttggcataga aacaacagaa
      421 acatctttgc ctttttcagc caattctttt tgagctttca cagccaaatc aacttcagaa
      481 cctgttgcaa tcaaaatacc ttctggtgtt tctccttgtg atggagaaag aacatacgcg
      541 ccttttttca ccatttcatc tgctacttct tttgtagaag gcaatactgg aaggttttga
      601 cgactcaata caaggatagt aggcgcatct gttgtctcca tcgctacttt ccaagcagca
      661 cgtgtttcat ttccgtctgc cggacgaatc acttgaacac caggcatgca gcgaacagat
      721 gctagttgtt caatcggttc gtgcgttggt ccgtcttctc cgacagcaac cgagtcatgt
      781 gttaagacgt aaacaacagg agtatgttgg attgctgcta aacgaactgc tggtcttaga
      841 taatcgacaa agacaaagaa tgttccaccg tagatacgag ttccgccatg aagttggata
      901 ccattcatag ccgaagccat tgcaaattcg cggacaccga accagatgtt gcgtccttca
      961 taatgttcag gagtgaaatc tttatctgca gccaccattg tattattgga acctgacaag
     1021 tccgctgaac caccccagaa gcttggtata gctttcgata attcttggat gacttcttta
     1081 cttgaaacac ggcttgcttg actgcttcca acttcataag ttggcaattc tgcatcccag
     1141 ttttctggta gtttgccatc aaaagcatct tcaaattgtt gtgctaattc aggataagct
     1201 tttttataat tggcaaacat ttcattccat gcatcttcag ctttttgtcc ttcttctatc
     1261 atcgtttgat ggaagcgggc agctacttct tctggaacag tgaaatctgg atactcccaa
     1321 ccataaactt ctttagccat cttgatgcca tcttcaccaa gaggagcacc atgaacagca
     1381 gatgttcctt ctttaggagc accatagccg atgactgttt ttacttcgat caatgttggt
     1441 ttgtctgttt cagcttttgc ttcttcaatc gctttagaga ttgcttctag gtcatttccg
     1501 tctttcacta agatatgttg ccagccatat gcttcatagc gagcaccaac gttttctgtg
     1561 aaagcttttg aagttggacc gtctagtgaa atatcgtttg aatcgtataa aacgatcaat
     1621 ttccctagtt tcatatgacc tgccatcgaa cttgcttctt gagatacccc ttccatcaag
     1681 tctccgtcac cgcatattgc atacgtatag tgatccatca cattaaagtt ttctttgtta
     1741 tacgttgctg caaggtgcgc ttctgccatt gccataccga cagccattgc aatcccttgt
     1801 cctaatggcc ctgtagtggc ttctacacca tctgtatgat gtacttcagg atgtcctggt
     1861 gttttagaat cccattgacg gaattgtttc aaatcatcga ttgtcacttg atagccagct
     1921 aaatgaagca agctatacaa cattgctgac ccatgtcctg ccgaaagaac gaaacggtcg
     1981 cggtcagccc agttttttga tgtcgttgga tttactttca ggtgttttgt ccataacgca
     2041 tatgccatag gtgctgcgcc catcggcaat ccaggatgtc ctgagtttgc tttttggact
     2101 gcttcaatac ttaatgtacg aattgtgtta acgccaagtt ggtcaatttt gtcaaacaaa
     2161 ataacccctc ctattaattt aggttttttt tacctttatt tcttacactt tcattctata
     2221 agatgtcaca cgaaaaagca atctttttca gtatttttta ctaaaagttg tcatttcggt
     2281 tgtgtaagcc ttttttcttt tggatttctt ttaatttttc cggggtcacg tcattgccct
     2341 caggatcgac gactttcatt ccttcgatat gatgacgcat accggaacga aaagcttcca
     2401 aatactcttt gcgcaagttg atttgttctt gttcttcggc ttttgagaga ccattttcct
     2461 tctttttacg tgcaagttca ttgatccgct ctattttttc aggtgacagc atgctcagcc
     2521 ctccttttac tctccttcat attatataaa aactgaaaaa aaaacaacta gataaactaa
     2581 ataaaatacg aacattcgtt tgatttagcg ctttttatat ggtactattt atttatcaag
     2641 tgaaagaagg tgtaccttgt ggcccgacaa actgaatcaa gacaaatcga agtcttacga
     2701 tatatccatg aacaagtaac ccaaaaagga tacccgccga ctgtccgtga aatcggggaa
     2761 gctgttcagc tttcttctac ttctactgtt cacggtcatc ttgcacggct tgaaaaaaaa
     2821 gggtttatcc aaagagatcc gacgaaacct cgtgcaatcg agttgacaaa acaaggttta
     2881 gaaaagattg ggattcgtcc aacaactatt ccagtacttg gcgtcgtaac agctggagaa
     2941 cccattttag cagtagagga tgcttcagac ttctttccaa tccctcctaa tctttcttcc
     3001 gaagaaggca gtctctttat gctgacaatt cgtggagata gtatgatcaa tgctgggata
     3061 ttggatggtg acaacgtgat cgttcggaag caagaaactg ctcaaaatgg cgatattgtc
     3121 attgcaatga ctgccgagga cgaagcgact tgcaaacgat tctataaaga aaaaaacttt
     3181 ttccgtctgc agcctgaaaa cgacttgctc gaaccaatca tcttagatca agtatctata
     3241 ttaggacgag tagtaggact ttatcgtagt catatctatt aattgctact aattaaaaga
     3301 aaacttttta aaaagagttt gagacgttgg tctcaaactc ttttttcgcc cgggttcttc
     3361 ttctttcaaa tttttatcac ttccctcttt acaaaccgaa caaatattcg tatactaaat
     3421 atacgaacaa ttgttcgcat aaattaaaga ggtgagaatt catgaaatca atgagacgtc
     3481 ttggcttagt attaactgtt ttgtttttag gtattattat tggtaatttc ggcatacgtc
     3541 cagctttact tgtgtttgtt ccgttattta ctttgtggtt catgttgtgg gatgaaaaga
     3601 agtaccgtca atctgagcgg cgcagaattc atgaagatca tttctatcat gagccctact
     3661 acagatccag gcaatcataa taaggattga ctgctaatta ttcaaaagac aaga
//
LOCUS       DAJSBL010000128         3634 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.132, whole genome shotgun sequence.
ACCESSION   DAJSBL010000128 DAJSBL010000000
VERSION     DAJSBL010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3634
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.132"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            577..909
                     /locus_tag="ONT85_002845"
                     /pseudo
     CDS             577..909
                     /locus_tag="ONT85_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353765.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2479 domain-containing protein"
     gene            923..1072
                     /locus_tag="ONT85_002846"
     CDS             923..1072
                     /locus_tag="ONT85_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2379764.1"
                     /translation="MKVVYKSIKPYGFEQIIVNDEEHLPEECTEVEPPIPNWKPKFNY
                     WEGNK"
     gene            1069..1515
                     /locus_tag="ONT85_002847"
     CDS             1069..1515
                     /locus_tag="ONT85_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2379765.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGVRSDGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYEALEHENGNLKQLNAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            1517..1654
                     /locus_tag="ONT85_002848"
     CDS             1517..1654
                     /locus_tag="ONT85_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HCR2379766.1"
                     /translation="MFSFSDVKMMYDWGCFTDDQVRLFVPLCITDEEADKIINKDKSA
                     S"
     gene            1691..1984
                     /locus_tag="ONT85_002849"
     CDS             1691..1984
                     /locus_tag="ONT85_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2379767.1"
                     /translation="MINLGEWGMIAGSITAIVSLILLVIRPIAASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHTEKLVEHTQQIKTLFRERRK"
     gene            1981..2205
                     /locus_tag="ONT85_002850"
     CDS             1981..2205
                     /locus_tag="ONT85_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HCR2379768.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESEEGGNK"
     gene            2202..3221
                     /locus_tag="ONT85_002851"
     CDS             2202..3221
                     /locus_tag="ONT85_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition protein"
                     /protein_id="HCR2379769.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGANEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAAN
                     PTLSDGTNHSHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIY
                     RPDVNQAYNTSGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPKR"
     gene            3410..3483
                     /locus_tag="ONT85_002852"
     tRNA            3410..3483
                     /locus_tag="ONT85_002852"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3444..3446,aa:Ile,seq:cat)
BASE COUNT         1244 a          645 c          754 g          991 t
ORIGIN      
        1 ttactagatg acccttactg ggtaggtaaa acatcgttgg gtgagaatat tgcaaataaa
       61 ggtacgacgt tcccaattaa atctagcgcg tacgaaatat ataaaggtaa cacggaagaa
      121 gagcttataa taggtcaaac gtatactatc acgcttaaag gaacaaaacc cgcaagtcaa
      181 acctttgtag catataatta ttggaacgtt aattttggag aactaaaacc agttgaggga
      241 ttgacagacg tatggtctct aacattcaca ccaacgaaac ttgaaccggg tttgcctaaa
      301 gaccttcgca tttttcagtt gcctaaagaa acggccggcg catgccaaat tgactggctc
      361 aaaattgaaa aaggcgacac ccgaaccccg aatattagtc agtttaaata ctttggtgaa
      421 ggattgaaag atagcaacaa tccgaacgac tacagctggg acatcacgcc tgaatatact
      481 gaaaaaggct tgaatgatac ggttagtttg actgaaccgc aatccatcga aggattgaaa
      541 aatttcgaag atggtattca atcaaaagga aaatctgtat tgacatcaga cgacaacaaa
      601 tatgaagtcg taaccttaac agttacaaac gggaataccg gatcagcaaa gctttatcgt
      661 gaaggaaaaa ccgtcactat ttattttttt gcgttgaatg ggaaaagcag tggtggaaat
      721 gattcaacga tactaacaat tccagaaggc tatcggccac caattagttt tgagcaactg
      781 gttggctcga tagaccgttc tactttgaac agtgctcagt tatctattgg tgcagatgga
      841 gccattaaat ggcgaagaaa ctcaagttat ggatcagctt attcatttgt tatcacttat
      901 tcaatttaag ggaggaaatc taatgaaagt agtttacaaa tcaatcaagc cttacggatt
      961 cgagcaaatc attgtgaatg acgaagaaca tttaccggaa gaatgcacag aagtcgaacc
     1021 accgattcca aattggaaac cgaaattcaa ttactgggag ggaaataaat gaaaaacatt
     1081 tggaaatatg gccgtactgg cggagagtat gcaggaaaag tattggacga catgcttgta
     1141 tccgttcctt acacagatca gccaccgctc gaaggggttc gttcagatgg cgaaccgcta
     1201 acgattgctg atcagatgtt tgatcctaaa ttgaaccaat ggattatttt agcgaacgcg
     1261 ttagatcaca acgatttaaa caatctcaaa gcgatgtacg aggctcttga acatgaaaac
     1321 ggcaacctaa aacagctaaa tgccaaactc atgctaagcg atgtagcgat taaacaggaa
     1381 aatactgcat tgaaagaaaa agctgacagt ttagcacaaa tcaattcaaa aatgatgctt
     1441 gcttcgttac aaaatagcaa agacatttca gaaattaaag agcaactaaa tccagcttca
     1501 aaaggaggtg agtagtatgt ttagttttag cgatgtgaaa atgatgtatg attggggctg
     1561 ttttactgac gatcaagttc gactattcgt tccactatgc attacagacg aagaagcaga
     1621 taaaatcatt aataaagata agagcgcatc ttaattgatg cgtttttatt taaggtaaag
     1681 gagttgtcac atgattaatt taggggaatg gggaatgata gcaggatcaa taaccgctat
     1741 cgtttctttg attttattag taattagacc gattgctgca tctttctcga aaattactga
     1801 gactctttca aaagtaagcc acaatttaga tttgctgact aaagatttag aatcgagcaa
     1861 atcagatcga ttgatgattc atgaagaact aaagaaacac gatgaaagat tagatacaca
     1921 tacagaaaaa ttggtggaac acacgcaaca aatcaaaact ttgtttaggg agagaagaaa
     1981 atgaataata aaacgttcga agtactaaaa tggttcgcac tggtaattat tcccgcacta
     2041 gctactttcg tggggttagt tggtaaagcg ctcaattggc agtacacaga tatctgtgtt
     2101 gtcatcatta ctggttttgg cgcgttttta gggagtgtgt tgggtgtatc aaatcgaacc
     2161 tacaaaatgt tctcggctga aagcgaagaa ggaggaaaca aatgaaaaag aaaattacta
     2221 ttactgcgat gagcctgtta acggctcttt ttttattgcc aattaacgga tttgcctata
     2281 ctatcaacaa tgaatttaat ttgggcgcaa atgaaggtag ctcacaagta gcaaataatc
     2341 agtatatttt actgcatgaa acggctaatg aaacagcaac aggacgcaat gaagcgcagt
     2401 atatgcaacg ttcatggact agcgcttata ctgcttatat tgtgggagac ggcggaattg
     2461 tttaccaagt cggacaacct ggttatgtac agtacggtgc tggttcatat gctaatgcca
     2521 atagtcctgt gcagattgag ttacaacaca cacatgataa agcaactttt gaaaaaaact
     2581 ataaagcata cgttgaattg gctagagatt cagcaatgaa atatggtatt ccattaacat
     2641 tagacacgcc ttataaccaa ccaggaatca aatcacattt atgggtaact caaaacattt
     2701 ggggcgatca cactgatccg tatggatact tatcagaaat gggtgtaagt aaggaaaaac
     2761 tggcttacga tttagctcat ggatttacag acgaaaaccc aacgacttct gagaacaagc
     2821 ctgtcattga tccaacccga gcaggtgcag caaatccaac tttatcagat ggaacgaacc
     2881 attctcacat tgatcagttt ggagaaatcg aaaatgcgaa cttacacgtc gctggatggc
     2941 acatcgctaa ctataagtac gagtatattt tcattatgga ctacaatact gggaaagaat
     3001 tagctcgagt aagagctgat ggaatttata gaccggatgt aaatcaagct tataatactt
     3061 ctggaaatgt tggctatcat gtatctttta acatgcgtaa cttccctaat aagaaagtct
     3121 atgtcatgat gcgggcaacg aatgatccag agggaaacac taaaggcggt gcgcaagatt
     3181 tccatgacaa acgttggtat ttaaatattc ctaaacgata aaaatagctc ctcgttgagg
     3241 agcattacat aactatattg acaactataa aaatcattcg ataaaatagt gatgttatcg
     3301 catatcttca ctatcaccca taatagtcac actccaagct atgcgataac aggtttgttg
     3361 ccacacattc tactggttga ttgtttatgg ctttatgtgg caacaaccag tacccttagc
     3421 tcagttggtc agagcagacg gctcataacc gtccggtcgt aggttcgagt cctacagggt
     3481 acattaacgt agccatttga atcgttctgt gttagaattt tttgaagagt attatacaag
     3541 ctaaagcttt tcttcattgc cactcaaatg agtggctttt ttatgtatcc ttttatggat
     3601 taatgaaagg atgtttcaca tagttatatt tctg
//
LOCUS       DAJSBL010000129         3391 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.133, whole genome shotgun sequence.
ACCESSION   DAJSBL010000129 DAJSBL010000000
VERSION     DAJSBL010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3391
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.133"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(22..2493)
                     /locus_tag="ONT85_002853"
     CDS             complement(22..2493)
                     /locus_tag="ONT85_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HCR2379770.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGYQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKMIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            2766..>3391
                     /locus_tag="ONT85_002854"
     CDS             2766..>3391
                     /locus_tag="ONT85_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR2379771.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGKLDDSS
                     SYCEHCRSVRIIRNGSYTTRTQMLKVKEKLTILELKRTRFLCHDCGRTFSAKTDLVDE
                     HHQLTKELKQVILMELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSMNSVSGSMSFICVDGTTNQLFTILEDRRLYKLTQYFLRFPR"
BASE COUNT          999 a          686 c          609 g         1097 t
ORIGIN      
        1 tcttttttga gatacttttc gtcaattttc tcgtttaaat atcgatagaa attttttagc
       61 tacgcgacta aacggctttt gctctttttc atttttacgt tcaaacaaat attctaccaa
      121 tgtggtcaaa tgcctaaaaa tcgaatctgc cgagaaaaat agaatgctca acagcaaaag
      181 tgatacagct gagataccgg tgattgaaaa cagcttatgc aaaaagatga ccgaaaaaaa
      241 gttaccgcta gcacagaaaa gaatgatctg acttctgaat ttattcagcg gtgaacagat
      301 tttatagaga acgaggaacc ctactgcaat caaaagcatg gtagatgctg tagaaacatc
      361 tgttttattt agattcaaag tcaccccgca gatcaccaac gcaccgacta cgatcatatc
      421 tgttagtcca cctggcacag ctttttccat cacattcatg atgaatttcc ctctgattcg
      481 ctttttgttt tcttctagag caagcaagaa agatggaagt ccgatagtaa acaaactaat
      541 caatgtgatt tgtgatggtt ctaaaggata ggtcagagca aagatcactg aaaacagtga
      601 gagcaaaaag gagaaaatat tcttcactaa aaacaagctg gctgatcgct caatattgtt
      661 cacaacgcgc cgtccttctg caacaacttc tggcatgcgt gaaaagtcag aatcaagtaa
      721 gaccacttgt gaggcctgca tggcggcttc atttccggaa gccatcgcta tactgcagtc
      781 tgcttctttc atggctagaa tatcattcac gccatcacct gtcatagcta ctgtattgtt
      841 acgctttatc aattgaacga agattttctt ttgttctggc ttgacgcgtc caaaaactgt
      901 atatttttcg acagctgctt catattcttc ttctgataat ttagatgcat caacgtactg
      961 atcagcatgt gaaatccctg cttggacagc tacattggat acggtgagcg gattatctcc
     1021 agaaatcact ttgatggcta cttgctgttt tttgaagtaa tcaaatgtgg ctcttgcttc
     1081 ttttctgata ggattagcaa tcagaatata gcctaatggt gtgactgctt ctgtcaatac
     1141 tggttcttca agaatcccat cgtacacacc aaagaccaac acgcgatagc ctttttcagc
     1201 gaaagaaaca aactcttccg catattccgg caattgatcg cgcaaaacca tttcgggcgc
     1261 tcctaaaaca tacaccttgt ctgccaacgc tacactgctg aatttttgga cagaagaaaa
     1321 aggaataaca tggtgatagg attgatcagt ggtttttgta aaatatgctt taacagcttc
     1381 cattgttgca ttgtctcctg ccatcgcttt tgcatagtca gcaatgcatg cttccaattg
     1441 atctgactga ctgattattt cttgtttttt agagacgatc attttttgaa cagacatttt
     1501 attttctgtg atcgttcctg ttttatctac acaaaggaca tttacccttg ccaatgtctc
     1561 aatgcttttc atattgtgta aaagaactct ttgctttgct agtttagtcg cacttaatgc
     1621 taatgcaatc gtcgtcagca agtacagtcc ttcaggtatc ataccgatca atgctgcttc
     1681 cattgcaacg acactttctt tgatcgtgtt tccattaaaa aagtaacttt gagaaaaaag
     1741 caggataccg ataggaataa tgatgatccc aatccatttg atcaattgat taagagaagc
     1801 aatcatctcc gactgttctc cttctcccat tgtcttggct ttgactgtca gctgggaaat
     1861 atacgaatct gctccaactt tgtctaatcg ggcgtaagct tttccggaaa caatgaaact
     1921 gccggacatt aatggatcac ctatttcttt ggttatttca tctgcttcgc ctgtcaataa
     1981 agcctcatta acttgcagtt tcccttccac gacctctgca tctgctggaa tttgatatcc
     2041 agtctctaaa ataacaatgt catctattac aaggtcttct atatctattg tttgttctat
     2101 cccgtctcgt atgacattga cttttgctgt atttaaaaca tttagttcgt ccaagatctt
     2161 tttggatcga atttcctgaa aaatggcgat tcctgtattg gctatgataa ctggtaaaaa
     2221 agtcaagttc ttataggatt ctactaaaca aagcaaaatg gctaagataa gaaagatcaa
     2281 attgaaatac gtaaatatgt tttccaatac gatttgctga tttgttttga atgtgggatc
     2341 gactgaatta tttgctgcac ctttttctat tcgttccttg acttgatctg ccgttaatcc
     2401 ttctttatac gatggaaaaa gacggatgac tttatctgat gatctttgtg ttactttctt
     2461 ttttttactt cgatttatcc atttattctt catgtggcta cctcgattga tttcgttttc
     2521 tggatatttc caaacttctc ctatccagta tagaacagat atggaaagtg ttcctcacat
     2581 taagcttgca aacacctttc attcctgtca tatagcaaaa aaaaagggtt ctataatatt
     2641 ttgtgttggt ggaaaatcga aggcgatttt ctactaacac tttttcttcg tttcaactca
     2701 aaaagagact cacttttggt aaaatttagt cgaccaaaac gtcatcctac caaaggagtc
     2761 tctcaatgga taatcatact agaaaattac tcggattaac agataaacat ttattttttg
     2821 atgaagaatg gctgattgaa aaagaatata aaggcgttca agcgcagttt attaaaggaa
     2881 aattggatga ctcttcttcc tattgtgaac actgtcgatc ggttcgtatt atccgcaacg
     2941 gttcctatac gacacgcact cagatgttga aagtcaaaga aaagttaact attttagaat
     3001 taaaacgtac ccgttttctc tgccatgatt gtggaaggac cttttctgcg aaaacggact
     3061 tagttgatga acatcatcaa ctaacaaagg aactgaagca ggtgattctc atggaactgt
     3121 acgaaaatca atcacgtaaa ctaatcgcta agaagtactt cgtctcggat gggacggtga
     3181 cacggatctt gcgtgaagca acgaagcatt accagccacg tatgaacttc cttccgacgg
     3241 tgttgtgtat ggatgagttc aaatcaatga attctgtttc tggttccatg agttttattt
     3301 gtgttgatgg gacaaccaat cagctattta cgatccttga agatcgcaga ctttacaaac
     3361 taactcaata cttcctgcgc tttcctagaa a
//
LOCUS       DAJSBL010000130         3345 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.134, whole genome shotgun sequence.
ACCESSION   DAJSBL010000130 DAJSBL010000000
VERSION     DAJSBL010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3345
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.134"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            303..584
                     /locus_tag="ONT85_002855"
     CDS             303..584
                     /locus_tag="ONT85_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2379772.1"
                     /translation="MDILVRNINPKIITRIDKRCQELTTKTGKKWSRNQYLKILIEND
                     FDHELMNYKKDQFDLLFEKFIAIQAENTNILNDYVHTNNQLIETLIGGI"
     gene            586..1002
                     /locus_tag="ONT85_002856"
     CDS             586..1002
                     /locus_tag="ONT85_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2379773.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDVNNYSEAIRFLCMSY
                     EPATNTTTTSNQAKLNAMGKEISIMTEIMAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     ENQLNPSRRNQPKNRQKETNQTFTSGNFDPTNFLKR"
     gene            1654..2079
                     /locus_tag="ONT85_002857"
     CDS             1654..2079
                     /locus_tag="ONT85_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2379774.1"
                     /translation="MICKNKKKIIAIIASFSLIFSTACTVLLTNNANVEAASSSYKHN
                     HAGYRCYKMSYYVSPSKCKQLARQTNKLNTVSAIANFIGLSGLTPGIISLVFGSAVSA
                     NNVFVTAARRGKGVQLNYIAHFSNTTTYNYSSNQNYVIK"
     gene            2109..2273
                     /locus_tag="ONT85_002858"
     CDS             2109..2273
                     /locus_tag="ONT85_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2379775.1"
                     /translation="MKLTKIAVILAVIAGIILYFVLPANQFFLYFGIPAILIVNGVRI
                     FEQRKKCSST"
     gene            3231..>3345
                     /locus_tag="ONT85_002859"
     CDS             3231..>3345
                     /locus_tag="ONT85_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HCR2379776.1"
                     /translation="MANEIVKYHHELNTIPLRKFTSVEMNLFFSIVSRMRDV"
BASE COUNT         1130 a          583 c          567 g         1065 t
ORIGIN      
        1 cccatgttgc gatattttgg tggcgcccca atccccgttt tttcgttgaa tcttactcgg
       61 actgcactcg ttttacagct gatttttctg aattttgaaa gagcataagg aaacgtggta
      121 tcactttctt acgaaaggtg ctagataagc tgtgcctatc acgtttctca gaaaaaaatt
      181 tgctatcact tttttgccat tgtggctagg ctatgtatag tgttttggcg aaaaatagct
      241 atacaaatac tatatttgta tgatatacat aaattatata ctgatacgaa aggaaaattt
      301 ccatggacat tttagttcga aatatcaatc ccaaaattat tacaagaata gataaacgtt
      361 gtcaggaact aacaaccaaa acgggcaaaa aatggagtcg aaatcaatac ctaaaaattt
      421 taatcgaaaa cgattttgac cacgaactta tgaattacaa aaaagatcaa ttcgatttat
      481 tattcgaaaa atttattgcc attcaagcag aaaatacgaa catactaaat gactatgttc
      541 acacaaacaa ccaactcatt gaaacactta tcggaggaat ataacatgag aaaaacttct
      601 gttcaaaaac acgtaaccct ttatcccagt cacgttgacg ttttagattc catcatcgaa
      661 aaaaatccag acgtcaacaa ttattcagaa gccattcgct ttttatgtat gagttatgaa
      721 cctgctacca atacaaccac aaccagtaac caagcaaaac tcaatgccat gggaaaagaa
      781 atttctatca tgacagaaat catggcagaa tttgctgatt tatatctaaa agatactggt
      841 gttcttatcg gtacaaattc taaagtatat caagaagtga aagaaaaaat tgaaaatcaa
      901 ctcaatcctt cccgacgaaa tcaacccaaa aatcgtcaaa aagaaaccaa tcaaaccttt
      961 acttctggaa attttgaccc aacaaacttt ctaaaaagat aaaaaaagaa accgaaaaat
     1021 aaaaaaattc ctcgatttct ttttagggat cggtcttcca gcaagatttg caagttggta
     1081 ccaacttgcg aaaaaaaaaa gaaaaatccg cttctgcgaa ttcttctttt ttcattttgg
     1141 ggcgctgccc taatccctaa gtcccggtta ttcaatagca tggaaactca aagaataaga
     1201 cgttccctgg aataaaaaaa aggctgctcg ttcgagcagt ccatacatag gaaaatgaaa
     1261 aaacgacgtc ccttactgag aaacgctatt aaacttttaa caaatcctct aaaaaataac
     1321 aagtctttaa aaagtgaccg gtaaaaatgc acaaagaact aaaaaagaga aattgcatca
     1381 aatcaagcat tagaagctag taactagcta gaccaattga aaatcacaaa gtgagcgcct
     1441 gtatctattg ctacaggcgc tacttggcat agtgtacatt atgtcaacaa gaatttaata
     1501 taacatattt ttctagtttg ttataacaat gccactctaa caaaaatgta agcattatcc
     1561 ttattaaaag ctccttgaca aggactatac cttttatttt atgattttta catagaaaac
     1621 gttttctcta ttttttgttt agaggtgatt tttatgattt gcaagaacaa gaaaaaaatt
     1681 attgctatta ttgctagttt ctctttgatt tttagtactg catgtactgt cttattaaca
     1741 aataatgcta atgttgaagc tgcaagctct tcttacaaac ataatcatgc tggttataga
     1801 tgttacaaaa tgagttatta tgtaagtcct tctaaatgca aacaattggc tcgccaaact
     1861 aataaactta ataccgtctc agctattgca aactttattg gactatctgg attaacaccg
     1921 gggattatat cattagtttt cggatcagca gttagtgcta ataatgtatt tgttacagcc
     1981 gctagaagag gaaaaggcgt tcaattaaat tatattgccc attttagtaa taccactacc
     2041 tataattata gttctaacca aaattatgtt ataaaataat taaaacttta aactaggagg
     2101 aatgctttat gaaattaaca aaaattgctg taattttagc tgttattgct ggcattattt
     2161 tatattttgt attgcctgct aatcaattct tcctttactt tggaatacct gcgattttaa
     2221 tagtaaatgg cgtaagaatc tttgaacaac gaaaaaaatg tagctctacc taatcgttac
     2281 aaaacaacaa tttcaccttc ggtgaaataa gcgaaacttt acaaaaagtg gctagcaaat
     2341 gctagccttt tttgtattgt agagcgtgaa ttttgagggg gattcaaggg ggagcttgcg
     2401 cccctttgac aagcggttat ttgcaccaaa tacctccgta tttggctagc aatccttcta
     2461 aaaaatacac ctatcgatca gagtcaaaac aaatcaattt tcgagtgagc gaatccgaat
     2521 ttgcaaagct gaagctttgc aaatgagtgt gccggcttcc gtaaaaatac atatggctat
     2581 aatttgcgtt ttaaggcgtt ttaaacgttc tggtatataa ttataccaaa acaactttaa
     2641 aacgccttag atctaatttt agaggtctta tagaacattt tagggttgtt agggcttgcc
     2701 ctgaccgcct gtaaggcgct ttgagaacaa gataagggtt ttatactaag actgtcttaa
     2761 aacggcttaa acaaacgctc agaacttttc aaaggagttc tttatgaact gtttttaagc
     2821 aaaggagcct ttaggcgact gcgcagtata atgtgagagc gtagcgatca cacattttta
     2881 gtgaccgtag ggagcgagtt tcccagttta gaaaaattcg cgaacttgtt tgaggaaaaa
     2941 agcaggagtt ttttaggcgg gaaattttgc ctcctctttt ataatctctt tttaaacctc
     3001 tttttaaacc tctttaccgt cggctgtatg cttactctcc caaggtgtgc agaatctttt
     3061 gggggtcttt ttgtctgttt ttaggggtct ttttgtctgt ttttaggggt ctttttgtct
     3121 gtttttgggg gtctttttgt ctgtttttag gggtctttaa ttaggggctt gacccctatt
     3181 agaaagctct ttataatttt gttaagtagt taatcttgaa ggaggatttt atggcaaatg
     3241 aaattgtgaa ataccaccat gaattaaata cgattccttt acgcaaattt acgtcagtag
     3301 aaatgaattt attcttctct atagtatcta gaatgcgtga cgtag
//
LOCUS       DAJSBL010000131         3213 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.136, whole genome shotgun sequence.
ACCESSION   DAJSBL010000131 DAJSBL010000000
VERSION     DAJSBL010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3213
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.136"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..443)
                     /locus_tag="ONT85_002860"
     CDS             complement(<1..443)
                     /locus_tag="ONT85_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HCR2379777.1"
                     /translation="MTYTHLTPNELVMIEAYFHQETPVAIVAKQLKRGRQTIYNVYNF
                     LKCGGTALEYFEQYKENKRRCGRTEIIFPAEEKEYIAKRSTEGWTPDVIIGRAERTFS
                     CSVSTLYRRFKTGEFNVLHLPMQGKRKPNGYKEKRGKQAFKRNIS"
     gene            513..863
                     /locus_tag="ONT85_002861"
     CDS             513..863
                     /locus_tag="ONT85_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR2379778.1"
                     /translation="MSRLIIQTAFYKAIIPENLDEVKLYLWEDNGEIVGFSGVSAEEL
                     VMLFLDPDFIGKRYGSQILIELIETEDIKRIDVNSQNKYAKHFYLSHGFEIESEEDLD
                     GFGKPYPITHLVKK"
     gene            1277..2095
                     /locus_tag="ONT85_002862"
     CDS             1277..2095
                     /locus_tag="ONT85_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HCR2379779.1"
                     /translation="MNKNPHNLKLVAVDMDGTFARTDYTYDIPRFKAVLSRMKAVDCQ
                     FVVASGNQYYQLRSLFPNYYDELSFVAENGAFVKDKTEVLFSADMPRKAIEVVLDLCK
                     DYPEVKNVMCGLNSAYCERGKVSQAFFDLTNIYYHRLEWVADFAEVQDKILKFAPTMP
                     VEKTEFYVELFREKLAGLLVPTSSGHGSVDLILSECHKTFGLKMLVERLGINPDQCVA
                     FGDGGNDIEMLEYCGLSYEMDNATEAVKQVAKHQCPSNDEDGVLVTLDRLFPNT"
     gene            complement(2168..2527)
                     /locus_tag="ONT85_002863"
     CDS             complement(2168..2527)
                     /locus_tag="ONT85_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HCR2379780.1"
                     /translation="MSDNLFLEVQPEVFKQVPRLKDGFLLTLNDGSNQFSSAEGCCMI
                     GDHFLLVSTQHYIPAYDLTIPCEEATVKTSEYDTTFINGHLVLEVTPSNQTIRLKNES
                     GILDSNVQVYFDSSDAS"
BASE COUNT         1059 a          475 c          574 g         1105 t
ORIGIN      
        1 tcagaaatat ttcttttgaa tgcctgtttt ccacgttttt ccttataacc atttggtttt
       61 cgttttcctt gcatcggtaa atgtaaaaca ttgaattctc ccgttttaaa ccggcgatag
      121 agggtactta ctgaacaaga gaaggttcgc tctgcacggc caataatgac gtctggggtc
      181 caaccttcag ttgatctttt tgcaatgtat tctttttcct cagcaggaaa aatgatttcg
      241 gttctcccac aacgtcgctt attttctttg tattgttcaa agtattcaag tgctgttcca
      301 ccacatttga gaaagttata gacattgtaa attgtttggc gtccacgttt aagctgcttc
      361 gcaacgatgg caaccggagt ttcttgatga aaatatgctt ctatcattac aagctcgttt
      421 ggtgtaagat gggtataagt catttatgtt cactctcctt gtatgcttta gcgggtatta
      481 caatttgagt gtaacataaa tggctttttt atttgtctcg cttaattata caaacggcgt
      541 tttataaagc aatcattcct gagaacttag atgaggttaa actttattta tgggaagata
      601 acggagaaat tgtcggtttt agtggtgtaa gtgcagaaga attagttatg ctatttcttg
      661 atccagattt tattggtaag cgatatggta gtcaaatatt aattgagctg attgaaactg
      721 aagatataaa aagaattgat gttaatagtc aaaataaata tgcaaaacat ttttatttaa
      781 gccatggatt tgaaattgaa tctgaagaag acttagatgg ttttggtaaa ccatacccta
      841 taacgcattt agtaaaaaaa taattcatga atggtttatt acctgattga acatgaataa
      901 aagtaatatt tttattttga tggcctattt ctaggttatg aattatccag atcaaatttg
      961 tgtattacat tcagtaataa ggtgcttatc ttaggatcaa cttatgatac tagctgacca
     1021 aggatgtacc agttatagaa aatcagttaa agtaaaaatg ataaaaatct tatgtcaaca
     1081 ctgatttagg ctgacctgtg ggttaggaga aagacaggtc ggtagctttt aatacaaaaa
     1141 atggcttgta tgtatctaga tagttaactg aaattagaaa tgttcttatt tttaacggag
     1201 taagaaaaat aggctgcttt aggtctgaat actttttcaa tataatgaag atatcaaagg
     1261 cgacaggagt gtttttatga ataagaatcc tcataatcta aagttggtag cagtagatat
     1321 ggatggaaca tttgctcgta ctgattacac atacgatatc ccaaggttca aggctgtttt
     1381 atcgcgaatg aaagctgttg attgtcaatt tgttgttgca agtggaaatc aatactacca
     1441 attgagatcg ttatttccaa attattatga tgaattaagt tttgttgctg aaaatggtgc
     1501 ttttgttaaa gataaaacag aggtactatt ttcagccgat atgcctagaa aagcgatcga
     1561 ggtggtcctt gatttatgta aagactatcc tgaagtaaaa aatgtgatgt gtggtctgaa
     1621 tagtgcctac tgtgaaagag gaaaggtgag tcaagcattt tttgatctaa ccaatatcta
     1681 ttaccataga ttagaatggg tagctgattt tgcagaagtt caagataaaa ttttaaaatt
     1741 tgctccgact atgcctgttg aaaaaaccga attttacgtt gaattgttcc gtgaaaaatt
     1801 agccgggcta ttagtaccta ccagtagtgg tcatggttct gtagacttga ttctttctga
     1861 gtgccataaa actttcggct tgaaaatgtt agttgagcgc ttggggatta atcctgacca
     1921 gtgcgttgct tttggagatg ggggcaatga cattgagatg ttggagtatt gtggtctgag
     1981 ttatgaaatg gataatgcaa cggaagctgt taagcaagtt gctaagcatc aatgtccttc
     2041 aaatgacgaa gacggtgttt tagtgacgct cgatcgattg ttccctaaca cgtaaacact
     2101 aggtaattca tcaaaaaacc tcgatataag aaatattcga ggttttaaaa ctatatttag
     2161 aacagattta cgaagcgtct gatgagtcaa aatagacttg gacattgcta tctaaaatac
     2221 cactttcatt ctttaaacga attgtttgat ttgatggagt tacctctaat actaaatgcc
     2281 cattaataaa ggtagtatcg tattcagatg ttttaactgt tgcttcttcg caaggtattg
     2341 ttaaatcata tgctggaata taatgttgtg tggaaactaa taaaaaatga tcaccaatca
     2401 tacagcatcc ctctgctgaa gaaaattgat tagagccatc atttaaagtt aacaagaatc
     2461 catcctttaa acgaggtact tgcttaaata cttctggttg tacttctaaa aataaattat
     2521 cgctcatttt cacgaccacc atcgacttcc acagttgaaa ttgacatttt atttaacaca
     2581 cctattcccc cttaactata actcaattct atataaaaaa tattctattc tcaaataatt
     2641 gaacttttca aatcgccaat ttacttcggc tttatcagag ctcattcata tcaagagcga
     2701 ttaacgctgt aaaaattaaa ccgaagatct gataaatttt ctctattttc tacaatattt
     2761 aaaacgcttc cttagtctat tatatatcta tttctcttag gagtatgttt agatgtttga
     2821 ataatagttg cccaacttta aaggttattc ttataatatt ttacttcatt ttccaataga
     2881 aaattcaaag gattactggt ttttctaaaa tagtttagtt taaatacata agtatcctcc
     2941 gacactgtat tagttcataa tcatctaaat acaaaatttt caaataaaaa tatttcaagc
     3001 tctatcgttg acgaatgtaa acgatactta taaagtaata gaaattgatt acatttgtaa
     3061 acttaaaatg gttaatttat ctcacataaa actaagaata aaataatgat taaaagtggt
     3121 tagtttttat acgatttagc ctatcggtaa aaatgaatta gagagtgtaa aatattttgt
     3181 gtaaatgaaa aaatccatac aaaaaaggaa gtc
//
LOCUS       DAJSBL010000132         3202 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.137, whole genome shotgun sequence.
ACCESSION   DAJSBL010000132 DAJSBL010000000
VERSION     DAJSBL010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3202
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.137"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(419..1033)
                     /locus_tag="ONT85_002864"
     CDS             complement(419..1033)
                     /locus_tag="ONT85_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HCR2379781.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            complement(1639..2016)
                     /locus_tag="ONT85_002865"
     CDS             complement(1639..2016)
                     /locus_tag="ONT85_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2379782.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(2316..3197)
                     /gene="galU"
                     /locus_tag="ONT85_002866"
     CDS             complement(2316..3197)
                     /gene="galU"
                     /locus_tag="ONT85_002866"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HCR2379783.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
BASE COUNT          975 a          659 c          497 g         1071 t
ORIGIN      
        1 tagaaaaagg tatcctgttg gacaattttg ttatatcagg taaacttcat tgcaataatg
       61 ataacaaatg tactataatt actttgtaaa gagttttggg gaagactctt tcatcaacgt
      121 gtattaagag acaagaaaga atccatatgg attctttctt gtctcttttt ccactacttt
      181 ttatttttct gacactacat attcttataa ctcatttctc ataactgtga aacaacttat
      241 aaaaataaca aactttaaac aaaacaagct taaaatcaaa gatgatccat cttaaaataa
      301 ctaaaacaaa aaaagtagtc gttgttaatc ctcattaata atcgttttct ttttttcaaa
      361 cgctccattt ttatgtggaa cattattttt ttaaggaact ttgtcaacat gtggataact
      421 attttccact tgtgtcctgg actagctgtt ttgctgtaaa atacaaaact tgctgcagca
      481 aaaagatcgt tgcaagagag gctgttccgc agatcgctgg atctagattt ggcaaagtca
      541 agactgcatc atgtaaattg gcgtggttgt ccaaaaccaa atcagcaaat tcaaataatc
      601 gcaaaccact gctatgttgc gatttgatac ttcgtgacaa atcaaatcca gtaacaatga
      661 ttaatggatg accattttct ttgatccact gagctagttc gatgattgcc gggtttcttc
      721 cttcattcga aagaagaaga aaaatgtctt ctgatttgac agaaagctga tcaataattg
      781 cctgtcctgc accttctagc gtttcataga cacctccggc tggattttca atcaatatac
      841 ttggtacaag atccggggtt tgatcccaaa attcaaatgc tacaccactt agtgtgcggc
      901 tagcatatat ctgcaatcta ccaccttgat taatttgttt cgcgatccaa gtgccagctt
      961 gaagcatcag cggctcttct atccttaatt tctcttccaa ttggtgataa tagtcaaaat
     1021 aacataaact catttatctc tcccgcctag ctatacttga catgaataga aacagctgcc
     1081 taatctaagg aagtacataa aaaaacagta gttagacagt aacacatgtg gtctttgctg
     1141 tttcaaacac aaaaaaggca ttgaacgaat acgcccaata ccttgacata gcaaataaaa
     1201 caacgtgcag accatggaaa atatctacat aggggtagaa ttttcaccct agattaggta
     1261 caataaatta ggtcgatgca ctgcattgac tatggcaaaa tgatcggtca gggtgtatgt
     1321 gtgtgactgg tcattccctg attcttagcg ccaactaagg gtcagggagc ctttttttat
     1381 tcagctgttt ctatctgaat ttattgtaag taatctctta tccaagtgca agcaagaaaa
     1441 tacaaaaaaa cattggtatg atttatatat atataaataa cataaaaaaa ggaatggctt
     1501 atcccgatac aacaagcatt cctttaaaaa agattctttt atataagtga aaaaagcttt
     1561 tttccaccaa ttaaataaat ctctcatctc aatagtttat ccattatttt atcttttttt
     1621 gcgtatacct actaaatatt accgtcttct tctcgttctt ctactttgtt cgagagaatc
     1681 tgtactagaa aaagtagttg tatgtctacc ttttctgcct atccgagact ttcttttcat
     1741 atatgggata taaatgatta gatacagtac agcaaaaaat aaagcaaata cgcaagcttc
     1801 ccaactacct aacgccaatt caaccatcaa ggtagcaacc ccaacggatg ctgcatacca
     1861 gataaaaatc agaaacgttt ctttttttct gaccatgcga aacatgataa atagactaat
     1921 aagcaatagt ggcaacacaa atatccaatt gcttccttcc atcagccact tgagtatatc
     1981 aagcagcgac tgtgcaacag cgactaattt tcccatagtg aacacacacc tttttttata
     2041 attaaaaaat tcgccttttt attctactat gaatcttctg gcttggcttt catttctctt
     2101 atgtaatcat tttcttttat acaacttaaa aaaaaattaa accattagca taatattttc
     2161 tgaatctatt cacgaaagtc tttttctatc tttcgttttc cacgactcaa ctgatctaga
     2221 gtcaaaaaac taaaacgaca gttgcgcaaa tgacgcacaa gctgccgctt taataacaaa
     2281 tagcctttca ttctcagcag gccacttatt tccagttaac tttctgttct tagcttttta
     2341 ctcaactgga taatatatgc acttaatgaa gtcccaattt ccgaatgttg aagtccatat
     2401 tcaatatttg tcttcatata tcctaactta tcgcctacgt cgtatctttt tcctttaaac
     2461 tctctcgcat agaccgcctc tgtctggtta agagtctgaa gtgcatccgt tagctgtatc
     2521 tcgtttcctg ctcctggttc ttgagtttct aatatctcaa atattttagg agtcagtaga
     2581 taacgtccga taatggctaa attacttggt gcttttccca tttcaggttt ttctacaaac
     2641 ccttttactc tgcaacgacc cttttcgcta tctgttttag gatcaataat tccgtattta
     2701 gatgtttcat tttctggtac acgcatgacg gcaacagcgg aagcttgtgt ttcttcataa
     2761 tccatcatca attgttgagt caatgggata tcatctttca tcaaatcatc ccccagcatg
     2821 acgacaaacg gttcatttcc tataaatgct tttgcttgaa gcacggcatc ccccagtcct
     2881 cttggatgag attgacggat gaagtgaaga ttgacttgag tcgtttcttc aactaaagcg
     2941 agtaattctg ttttttcctt ttcccgcaaa ttgttttcca attcgatatt tgcatcaaag
     3001 tgatcttcaa tgggtctttt ttcttttccc gttacgatca agatgtcttc gattcccgaa
     3061 tccaatgctt cttccacaat aaactggatc gttggtttat ccacgattgg aagcatttct
     3121 tttgccatgg cttttgttgc cggcaaaaaa cgtgttccta gcccagcggc aggaatgact
     3181 gcttttttga ccttcacttg ct
//
LOCUS       DAJSBL010000133         3198 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.138, whole genome shotgun sequence.
ACCESSION   DAJSBL010000133 DAJSBL010000000
VERSION     DAJSBL010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3198
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.138"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            12..3161
                     /locus_tag="ONT85_002867"
     CDS             12..3161
                     /locus_tag="ONT85_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HCR2379784.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWSNLRQ
                     KIISNNQAELNDSPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIERENPQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIIQIELK
                     QVTAKDGFYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNKKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGVSSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSPEQINRMIDQMESVKKESYLDPTVFEAEEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKESDWLIKEFPNQPIREGRTM
                     EDPDFPRIAITYSLEENSRDSSAQQEEMQKIIEEYNQYYDTAWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLVIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PEKTKGLIVTFRKPATMEKNVEDATKLYSEAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVQSSTLNNQIQLLEE
                     QIGVYETVKGSLMEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSD
                     AREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDNLIHA
                     FSNEWFVSEKELHASNLQYMPGEDPIPNMKAIINSKDYEGYKAKHPEAKPFKYPQEMK
                     RAWRKMLDDELIPLENELR"
BASE COUNT         1126 a          543 c          686 g          843 t
ORIGIN      
        1 agggaagaag aatgaaaatt agtcaccgtg aggaagcgga agtcgaagaa cagttgattc
       61 gtgtcctcgg ggaaggacat aatcagtgga cgtatcgtcc ggatctaaaa tcagaagaag
      121 atctttggag caatttacgt caaaaaatca tctcaaataa tcaagcagaa ctgaatgatt
      181 ctcctttgac cgataaagag tttgaaacga tcaaaaccga attattgcta cggacgaaaa
      241 ccccatttga tgctgcgaaa tggttaaaag gtgaaaatgg gatggctcgg atcacgattg
      301 aacgggaaaa tccgcaattg ggttctgtct cacttatcct ttattccaat caagacattg
      361 gtggaggaat ctctacttat gaagtggtcc atcaaattgc aaaacgtgga agtaacatag
      421 aagcgcggga tcgacgtttt gacgtcacct tattgatcaa tggcttaccg attatccaaa
      481 tcgagctaaa acaagtaact gcaaaagatg gtttttatca ggcgtttaat caaatcaaaa
      541 aatatgctga agaaggcatg tttcggaaca atatcttttc gaccttacag ttgtttgtgg
      601 tttccaatga acaaacgact cggtattttg caaatgcctt accgaaggat ttgcatccta
      661 agtttttatt tagttggcga acgaaagata ataaaaaggt agaaaactta tatgagtttt
      721 gcaaacaagt gctaaatatc ccagatgccc atcgcttgat tgcggattat acgattgtca
      781 gtgaagatca agacaataaa accctcatgg tgttacatcc ttatcaagtg catgcgattc
      841 aagccttgtt tattgccgca aataaacatc aatcagggta tgtgtggcat gcgactggtt
      901 caggaaagac gttgactagt tttgtatcta ctaagctttt agcacgaaaa tcggggatcg
      961 atcgaacgat catgttagtg gaccgcaaag atttagataa tcagaccacc acagagttta
     1021 cgaaatttgc ttcggagttt aatactgggg tttcttctgg taatgccaaa gcgaatagct
     1081 tgatcgtggg aacaggaagt gccaaagagt taagtgaaac tctgttggca gatgccaatg
     1141 cgaatgttgt gatcattaca acccgtcaaa aactagatgc ggcgttgaaa tatgcaaaga
     1201 aacaggaaga gaaaaaagga accaatcgct tccaaaaatt aatggggcaa catatcgtgt
     1261 ttgtagttga tgagtgtcat cgagcgttaa gtgctgaaaa tatggaagaa atcaaaaaaa
     1321 tgtttccgaa atccacttgg tttggcttta caggtacacc gattttccct gagaatcgga
     1381 aacaagccaa agggcaattg gcacgtacga cccatgatca gtatggagag gtcttgcaca
     1441 cctataccat caagaatgca ttagaagatg gctcggtttt aggatttcaa gtagagcatg
     1501 aaaacaccgt ggagccgact tcattagaga ataaaatcta tcggaaatta aaagaagtag
     1561 aaacatatgc agagtattca ccggaacaga tcaatcgtat gatcgatcaa atggagtctg
     1621 tcaaaaaaga aagttatttg gaccctactg tttttgaagc agaggaacat atccaaaaag
     1681 tcattcataa aatgttccgt ccagacaatg cctatacaaa atttgatttt cgaaatggcc
     1741 gtccaacgaa gtctgcgatc ttaaccacga gttccattga tatggccaaa aagtattatc
     1801 gggccatcaa agagatgacg aaagaatctg attggttaat aaaagagttt cccaatcagc
     1861 ctatccgtga aggacgaacg atggaggatc ctgatttccc acgaatcgct attacctatt
     1921 cattagaaga aaatagccga gattcaagtg cccaacaaga agaaatgcaa aaaattattg
     1981 aggaatataa tcagtattat gatactgcct ggtcactagc agacattgag cgttataacg
     2041 gagacatcaa taatcgtttg gcacgaaaaa gagcggaatt caaacaattt gggaaacaaa
     2101 tcgatttagt aatcgtagtg gatcgtctat tgacaggttt tgacgcccct acgattcaaa
     2161 ccctatttgt ggatcgtaat ctagagtatg caggattgat ccaagccttt tcacgtacga
     2221 accgtacgta tcctgaaaaa acaaaaggac taattgttac tttccgaaag ccagcgacaa
     2281 tggagaagaa tgtcgaggat gcgacaaaac tgtattcaga agctaaagaa gaatcgggtt
     2341 tgatttatcc aagttaccaa gaatccaaaa aaagattcaa gcaagcatat ggtaagttaa
     2401 aagaggtttc atccatggaa tccattgatg aacatacgcc acttgaaatc cgtatcgagt
     2461 acgtcaaagc atttcaagaa ttgaatcgtg cgtatgaatc gttagtgact tatgatgatt
     2521 acaatgatga catggtgcaa tcttcaactt tgaacaatca aattcaactt ttggaagaac
     2581 aaattggcgt gtacgagacc gtgaagggtt cattgatgga ggaagaacca gaaaaagagg
     2641 gagaagattt ttcaacgatc gaattttata gtgaaaatac cactaagttg tatgatattg
     2701 attctactta tattgatcaa ttactaggaa cttatgctgc gaatagttca gatgcacgag
     2761 aagaaattga aaaagctttg gctaaattga ataaaacaga aggagtcaaa gaagtttacc
     2821 gacaaatttt aaatgcattt gatgtgggga ctttagatag taatgaagat atctttgtca
     2881 ttaaacgtcg ctattttaca caagcaagtg ataatttgat tcatgccttt tccaatgagt
     2941 ggtttgtttc tgagaaagaa ttacatgctt ctaatctgca atatatgcca ggtgaagatc
     3001 caattcctaa tatgaaagca attatcaaca gtaaggatta cgaaggatat aaagccaaac
     3061 atccagaagc gaaaccattt aaatacccac aagaaatgaa gcgagcatgg cgtaaaatgt
     3121 tagatgatga gttgattcca cttgaaaatg aattgaggta aacgagaaga gttaagaaaa
     3181 ttgtacgatt caaagata
//
LOCUS       DAJSBL010000134         3128 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.139, whole genome shotgun sequence.
ACCESSION   DAJSBL010000134 DAJSBL010000000
VERSION     DAJSBL010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3128
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.139"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            103..1347
                     /locus_tag="ONT85_002868"
                     /pseudo
     CDS             103..1347
                     /locus_tag="ONT85_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729806.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
     gene            1360..1992
                     /locus_tag="ONT85_002869"
     CDS             1360..1992
                     /locus_tag="ONT85_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HCR2379785.1"
                     /translation="MTMGVIFDMDGVLIDSETFYFERRMNFFQELEIDPGSNQIDDYV
                     GKTEAAIWETLVPEDKSLREELYPKYVDYRKTHPIDFAKALRKEVKPLLDFLKKRDIP
                     LALASSSPAVEIESMLIQCELTEYFSFVISGESLKQSKPHPEIYLKAKQQLDCDFFIA
                     VEDSTIGIKSAKAANIYTAALKQPMQIDQSEADIQIESLQELEQIVLEKG"
     gene            2197..2424
                     /locus_tag="ONT85_002870"
     CDS             2197..2424
                     /locus_tag="ONT85_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011837604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HCR2379786.1"
                     /translation="MLQQNQNTNSYQAAILTAVNLGGDTDTTAAVTGALAGILYGGEV
                     IPENWRKSLQNDQLLKTISQRFCDTVLSQRI"
     gene            2493..2759
                     /locus_tag="ONT85_002871"
     CDS             2493..2759
                     /locus_tag="ONT85_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HCR2379787.1"
                     /translation="MLVQMMNGGRIMSQSTITFRIPDDEKELVLEYAKAHNSSLTELY
                     RNAVLDKIEDEIDLRTLRSAIEVSKEKKESGISQEEMEKLLNEL"
     gene            2749..3018
                     /locus_tag="ONT85_002872"
     CDS             2749..3018
                     /locus_tag="ONT85_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HCR2379788.1"
                     /translation="MNYKVIYMPQAQKQLKKMDRNQAKIIVAWIRKNLENTTDPRQHG
                     KGLTGNKSGEWRYRIGNYRILANICDSEIEIQIFSIGHRSTIYKR"
BASE COUNT         1101 a          466 c          617 g          944 t
ORIGIN      
        1 tgaaattgct ttcctttaaa atgactcatc gtcattcctc ctgctatctt tttctattat
       61 tctaccttat ctgatagtag atttaaaact ttgcaacaga accagaaaac caaagatcag
      121 cttaacgaat tttttgctaa tgttcttgaa accccttatt ggtatgcaaa aaaaatctta
      181 agccttttat ctgagtgttt agatcaacct gtgtatgaag aggacatttt ctttattacg
      241 ctatattttt atagtttgaa tgtcacgaat gttaaaacag gtattaagag tattgtgcta
      301 gctcatggtt attctacagc tagtagtatg gcgaatgttg ccaaccgtat gctgaataag
      361 aacgtttttc aagcttatga tatgccttta gatattacat tagagaaaat tgaagcaaaa
      421 ttgattcaat tccttggaga ttatcgaaca gattcaggct tgattttatt ggtagatatg
      481 ggttcactaa accaactagg cggtttatta gtgaatcatc taaatggacc actgttaatc
      541 attgatcata tcaatacacc gttattattg gaagtcggcc aacaagtatt aaacaataaa
      601 agtatcaccg aaataggagc agaaattgaa acaagtctta ctttcaaaaa gcagttacta
      661 cttcccaaga aaaagaaaaa gaaagccatc attacttgtt gttatacagg gattggtagt
      721 gcgacccaga ttcaggagat tttaacgaaa tgcttgaaag atgcagttag agaattagta
      781 attattccgt atgattacaa aaagctgtta gaaaacaaga attttgagac tccttttcaa
      841 ttatacgatg tcatcatgat tgtaggtaca gaagatccca aaattaatca gattccatat
      901 attggattag atcgcctgat taatggggga agcattggcg aatttgttca ttacttggaa
      961 aaagagctga ctattgaagt gaacgattta caaaaagaat taattttcaa tttttcaatt
     1021 agtaaaattg tcgaaaattt aaccattttg gatccagata aggtgctatc ttttgtacaa
     1081 aaagcaatta aagaattatc gaagttatta aattttgatt tttctaataa tcagctgttt
     1141 ttgttgtaca ttcactgtag ttgtatgatt gaacgaattg tacgtaaaga aagtgtggat
     1201 gagcaagagg acattgaaga atatatgcaa gcaaatggag aaataatgga taaaattcat
     1261 agcgcttttt ttgagattga aagggaatac acaattgaaa taccagattt agagttgcga
     1321 ttattgagcg atattgttca aggataggag tgaattcgaa tgacaatggg agtaattttt
     1381 gatatggatg gggtactcat agatagcgag acattttatt ttgagagaag aatgaacttc
     1441 tttcaagaac tggagataga tccaggatca aaccagattg atgattatgt ggggaaaaca
     1501 gaagctgcaa tatgggaaac attagttcct gaggacaaat cattaagaga ggaactttat
     1561 ccgaaatatg tagattatcg aaaaacgcat ccaattgatt tcgctaaagc tttacgaaaa
     1621 gaagtgaaac cgttacttga cttcctcaag aagcgggata taccattggc actggcatct
     1681 tcttctccag ctgtcgagat agagagtatg ctcattcaat gtgaacttac tgaatatttt
     1741 tcctttgtta taagtggtga gtcattaaaa caaagcaaac cacatcccga aatatattta
     1801 aaagcgaaac agcaattaga ctgtgacttc tttattgcag tagaggattc aacgattggt
     1861 attaaatcag caaaggcagc aaatatctat acggcggcac taaaacaacc aatgcaaatc
     1921 gatcagagtg aagcagacat acaaattgaa agtctacaag aactggaaca aattgtttta
     1981 gaaaaaggtt agtgaattaa tggaaataag gctatatatc tgtgctagct attttgacta
     2041 accaaaaaaa cttgctataa ttcaggtgta gtaaaaggtg ctggtaacac cttttacgaa
     2101 aatgcttcgg cagtgataat gggattggct ttttaagttt tttattttgt tcgcaatccc
     2161 taaggtcatg tgaatgatcc tagggattgc tagaatattt tgcaacagaa ccaaaatacg
     2221 aatagttatc aagcagccat attgactgcg gtcaatttag gaggggatac tgatactact
     2281 gctgccgtaa ctggtgctct tgcgggtatt ctttacgggg gtgaagtgat ccctgaaaat
     2341 tggagaaaat cgcttcaaaa tgaccagctt cttaaaacga tcagtcagag attttgcgat
     2401 accgtgctat ctcaaagaat ttgaataatg gatatgatgc aaatcttgtt tgtactaacg
     2461 ttgtattgac aaaaaggatt ttatttagta caatgttagt acaaatgatg aacggaggta
     2521 gaatcatgtc tcaatcaacc attacattta ggattcccga cgatgaaaaa gaactggtat
     2581 tagaatatgc aaaagcacac aattcttcat tgaccgaact ttatcgaaat gctgtactgg
     2641 ataaaatcga agatgaaatc gatctacgga ctttacggag tgcaattgaa gtttccaaag
     2701 agaaaaaaga gagtggtatt tctcaagagg aaatggagaa actgctaaat gaattataaa
     2761 gtcatctaca tgccgcaagc ccagaagcaa ttaaaaaaaa tggacagaaa ccaagctaaa
     2821 ataattgtcg cctggatcag aaagaattta gaaaacacga ctgacccgcg tcagcacgga
     2881 aaaggtttga ctggaaataa gagtggggaa tggcgctatc gaattggaaa ctatcgaata
     2941 ttggctaata tttgtgattc tgaaattgaa atccagatat tctcaattgg acatagaagt
     3001 acaatatata aaagataacc gtttttggaa cgaagtagag tttcgaaagt caatgtataa
     3061 tcttttgaat tttgcataat accacaggta gaaactaccc gatactatcg gttggtttct
     3121 gctttttt
//
LOCUS       DAJSBL010000135         3114 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.140, whole genome shotgun sequence.
ACCESSION   DAJSBL010000135 DAJSBL010000000
VERSION     DAJSBL010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3114
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.140"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(1..940)
                     /locus_tag="ONT85_002873"
                     /pseudo
     CDS             complement(1..940)
                     /locus_tag="ONT85_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173818.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
     gene            complement(1161..1523)
                     /locus_tag="ONT85_002874"
     CDS             complement(1161..1523)
                     /locus_tag="ONT85_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2379789.1"
                     /translation="MGMTLADIKAQEEKLKKEQAKLRQQKKRLIEKENAAMGKALRKR
                     FGFETFADFERWLSSISDDQQHEKSVDNEWLADEKSAPSERDYPDESDGLFGVIPNKK
                     SQMSESKFTSIFNDVNLR"
     gene            complement(1527..1865)
                     /locus_tag="ONT85_002875"
     CDS             complement(1527..1865)
                     /locus_tag="ONT85_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2379790.1"
                     /translation="MVAILEVRCDMDKIDLREETVDRIELMYERLADYLNLPDEDNYS
                     EMLERIMDVLNSVINADLAKSSVLTNEFVHKHLAQTTDESEKEFWLKLMSHVMQLNQI
                     SVINSKEKEM"
     gene            complement(1844..2263)
                     /locus_tag="ONT85_002876"
     CDS             complement(1844..2263)
                     /locus_tag="ONT85_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2379791.1"
                     /translation="MTDLSEKMTIKNLADELGVSKNTVRNQLVNSGFDLAQYKENGII
                     MLDKELIGVVRSIYRSKSDQLTGQLPVNNTGSDFPLEKENEFLRDQLAEKDKHISELT
                     EMIRDQQKLTLLQQQKNDQLLLENQELKNRKWWQFWK"
     gene            complement(2256..2489)
                     /locus_tag="ONT85_002877"
     CDS             complement(2256..2489)
                     /locus_tag="ONT85_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2379792.1"
                     /translation="MTEKQKYFGKKNWLVNLTGDYVVVVFLDSKKMSGKLLDVTQYEL
                     ILKNNKIDSPIIIPKHAVKYIYAVAESSGEAND"
     gene            complement(2486..>3114)
                     /locus_tag="ONT85_002878"
     CDS             complement(2486..>3114)
                     /locus_tag="ONT85_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HCR2379793.1"
                     /translation="SQFLASSMKNFIHDLFIEPHFSRADIACDIIDVPDEFITQYRVV
                     DPVSFKPIYGRSGKLETAYWGSRASERQIRMYNKKLEQERKRKIVPPEITTWWRLELQ
                     LRRGKATDWHEMVHESLDSFASPHYFPPNTKVTERIMVKGLLYDHSEWGNLAKNTKKK
                     YRDLLKQESQNDELTNHLRETFAESADDLKKELDTWLLGLDVTEEEEK"
BASE COUNT          868 a          701 c          433 g         1112 t
ORIGIN      
        1 atatcttttt ggaaaatgac cgccatctct ttttttacat tcagcatatt tttcttctct
       61 tcttcaatct tcactctctc agaagccacc agagcctttt ctcgctcatt ttcttctctt
      121 tgcctagaaa aatatacctc aactcatcag aaacgctctc acgctctttt acggccgttt
      181 gacgcaattc tgaaatctcg ctcatgattt ctttatattc gtgaacatct ttgattttgt
      241 tagtaccgac taactcacgc tcccaaccaa aatctgccat caaacgttca attgactcaa
      301 cttcctgatt cctaaaatta ttccaaatca ctcgaccatt ctctttatct gctcgtaaac
      361 cctgctgttg caaggctttg ttaaaacttg gttgcttttg aacaccccgt ttataccctt
      421 cagcaacagg aacaatattc aaatgcaggt gcggacttac ttcgtcatta tgaataacag
      481 cattgtagac ggataaatta ggattccgtt ctttaaattc tttgaaatat tcaactaaaa
      541 ttttgttggc aatcactcga ttttgttctg cttgttgttc cgatatgcca gtagaagaac
      601 catcatcacg agtagtacga aaatcatcaa gagtaccaat ttgcacgata aattctcgct
      661 gtggttccaa ttttttatcc ttgcgaacct ttgctaaata gtcctcgatt tttctatcgg
      721 ctcttttctg ctttgcatta tatttttcta cggcttcacc gaataattct tcatattttt
      781 cacgaatatt tgtttgttct aaatagatat tttcatccga ttttgtgaaa tcaatatgct
      841 tatgccagtc attgattttt tggctatcag ttaactcacg attattatga gaaatgcttg
      901 tattctttgt tccctttttg aacgaaatac tgaaccccaa gttaaaaccc tccttcggct
      961 gtcgctaata aaaagagtgt aaacctttct gttactcttt gtcaaagagt aacattttcc
     1021 taattgtgac atggctgtca caattccaaa tgctctccga gggtattcgc tgtcacaagt
     1081 gacaacgaaa aaaggagccg aaagcgtgtc acgctctcaa ctccttttac gctttatttt
     1141 gcttcacgca aaataatcgc ttatcgtaaa ttcacgtcat taaatattga cgtaaattta
     1201 ctctcactca tctgactttt cttattcggt atcactccga ataagccgtc agattcatcg
     1261 ggataatctc gttcgcttgg cgctgatttt tcatcagcaa gccactcatt atccactgat
     1321 ttttcgtgtt gctgatcgtc agaaatcgac gatagccacc gctcaaaatc agcaaaagtt
     1381 tcaaacccaa aacgcttccg aagagccttg cccattgcag cattttcttt ctcaatcaag
     1441 cgttttttct gttggcgaag tttcgcttgt tcttttttta atttttcttc ttgcgcttta
     1501 atatctgcta aagtcattcc cataaactac atctcctttt ctttggaatt aataacggaa
     1561 atttggttta actgcataac atgactcatt aatttcaacc aaaattcttt ttctgattca
     1621 tcagttgttt gcgctaaatg cttatgaaca aattcattag tcaaaactga agattttgct
     1681 aaatcagcat tgataaccga gttgagaaca tccataattc gttcaagcat ttcactataa
     1741 ttatcttcat ccggcaaatt caaataatca gctaaccgtt catacatcaa ttcaattcta
     1801 tcgactgttt cttctctcaa atcaatttta tccatgtcac acctcacttc cagaattgcc
     1861 accattttcg attttttaat tcttgatttt ctagtaacaa ctgatcgttt ttttgttgct
     1921 gaagcagggt caatttttgt tgatcacgaa tcatttctgt taattcagaa atatgtttgt
     1981 ccttctcagc taattgatca cgcaaaaact cattctcttt ttctaaagga aaatcactgc
     2041 ctgtgttatt gaccggtaat tgaccagtca attgatcact ttttgaccga taaattgacc
     2101 gaacaacacc aatcaactct ttatctagca tgataatgcc attttcttta tactgagcaa
     2161 ggtcaaaccc tgaattgacc agttgattgc gcactgtatt tttcgataca cccaattcgt
     2221 cagcaagatt ttttatggtc atcttttctg acaaatcagt cattagcttc tccactactt
     2281 tcagctaccg cataaatata tttaacggcg tgcttaggga taataatcgg agagtcaatt
     2341 ttattatttt tcaaaatcaa ttcatattgc gttacatcaa gtaacttacc ggacattttt
     2401 ttgctatcca aaaaaacaac cacaacgtaa tcacctgtta aatttaccaa ccaatttttc
     2461 ttaccaaaat acttttgttt ttctgtcatt tttcttcctc ctctgttacg tctagcccta
     2521 gaagccatgt gtctaattct ttttttaaat cgtctgccga ttccgcaaag gtttctcgca
     2581 aatgattcgt caattcatca ttttgacttt cttgcttcaa caaatccctg tattttttct
     2641 ttgtattctt agctagattt ccccactcag aatgatcata aagcaaacct ttcaccataa
     2701 tccgttcagt aactttagta tttggcggaa aataatgtgg actagcaaag ctatccaaac
     2761 tttcatgaac catctcatgc caatcagttg cttttcccct tcgtaactgc aattctagtc
     2821 gccaccatgt cgttatttcc ggcggaacaa ttttacgttt tctttcttgt tccagttttt
     2881 tattatacat ccgaatttgt cgttcactgg cacgactacc ccaatatgcc gtttctaact
     2941 taccagaacg cccataaatt ggtttaaaag acacaggatc aactacccga tattgtgtaa
     3001 taaactcatc aggtacatcg ataatgtcgc aagcaatatc cgcacgtgaa aagtgaggtt
     3061 cgataaacaa atcatgaata aaatttttca tggaactagc taagaattga ctaa
//
LOCUS       DAJSBL010000136         3087 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.141, whole genome shotgun sequence.
ACCESSION   DAJSBL010000136 DAJSBL010000000
VERSION     DAJSBL010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3087
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.141"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(25..162)
                     /locus_tag="ONT85_002879"
     CDS             complement(25..162)
                     /locus_tag="ONT85_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HCR2379794.1"
                     /translation="MKRGIVMGIVKDIFLVFGGSMLGVTVMCLMNASAAADRAMEKKG
                     K"
     gene            complement(159..1343)
                     /locus_tag="ONT85_002880"
     CDS             complement(159..1343)
                     /locus_tag="ONT85_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HCR2379795.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     TASIKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDARKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPELTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAFHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNRTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     II"
     gene            complement(1602..1892)
                     /locus_tag="ONT85_002881"
     CDS             complement(1602..1892)
                     /locus_tag="ONT85_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2379796.1"
                     /translation="MVYDELIGEIYWVIGKIQSDPELEEQLRRLKFDIRKNGVKVAGD
                     PYLMNEETDARIEINQVIAEFERIADQAKEPDIRQYLLEMKAELEVNGITDE"
     gene            complement(1882..3024)
                     /gene="dcm"
                     /locus_tag="ONT85_002882"
                     /pseudo
     CDS             complement(1882..3024)
                     /gene="dcm"
                     /locus_tag="ONT85_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
BASE COUNT          842 a          739 c          540 g          966 t
ORIGIN      
        1 aaattttgtc cgaaattcat cttgtcactt tcctttcttt tccattgctc gatccgctgc
       61 tgcacttgca ttcatcaagc acatcacagt aacaccaagc atactgccac caaataccaa
      121 aaaaatatct tttacaattc ccatgacgat tcctcttttc atataatcaa attttcaatt
      181 gccatacttt gttgctccaa aatcttttta tgtcggtctg ataagcgtgc attctgtacc
      241 atatcctgga tataggttgt ccgattcaat cgatccaact ccttagccat tttccacgta
      301 ggggcgactt gtcggcctag ccaattcaag gttcgttcaa tggtatacgg ttttggctcg
      361 gaagttaatt gaatctcttt ccgattccgt ccaataaaac gcccccattg ttcattcaag
      421 ggccagttgg ttcgcctttt accttctact ttatccgcaa atcgcaagta acgattgata
      481 atggaaaacg tggtgctctc aatgctacga ccttttaaca aatcttgaat cgcatgaaaa
      541 gcacggtcat ttttcagtcg aatctcaaaa cgatttttcg tctcagtttg gtctaatgga
      601 atccctaact tgatatattg ttcatagtct ttttcataca aacagaaata aacctcactc
      661 ttgagactgc cgatatataa ggtattgccc atgccgtcct tttcatcagc ctttaaaagt
      721 tccccggaac gataactttt aaaggtacga aacaaggaga tacattcttc cttctgacat
      781 tttttcgtta gctcaggtat atccaatagc cccactcgat cattgattgc caagtccaag
      841 cgtttcatca cgccaccggc ctttagacaa tcctcgaaaa agtcgtacca gctacgcttt
      901 tgagcgagta aaaaagtttc aaattgtcgg caacctcgcc cctttaattc taaaagcaca
      961 cctttatctt cttcatgaga caacatcacc acaatatcac ccatgacata ctgttcttga
     1021 taggagtaaa aggcataatc ttcatggagc atatactcaa atttcaaatg gagaatttct
     1081 tgaataattt ttcgtgcatc cgttgtggga aagcgaatcc ttacatagtc aaacagtaac
     1141 gtcaaaggca aatcgggatt aaagctttcc agttggtcaa aaattttcct catggttttt
     1201 atagatgcgg ttacgccacc attttctatc tgatttaagt acggccgact aagtccacaa
     1261 gccgtagcca atttattttg tgaaacgccg tattcgttgc gtcggtcttt taatagccga
     1321 taatctaaat tccgttgtgc caagttctca cctccaaatt caaggtgtta actttttcaa
     1381 atgagttaac acctatacaa attacttact atctttgata caacaagcat tcttaatggt
     1441 tgcttactta ttttttgtgt ttttgtaccc ccctgttaga tacgggggtg agacatcatg
     1501 gtcggctaac gccgtccatg aacaggcaca gcaccacgga ggatttctgc ttcgcgaatc
     1561 ctccgcggtc agcgcctaac agtcagttgt gttggtttag gttactcatc tgtaatacca
     1621 tttacttcca actctgcttt catttctaac aagtattgac gaatatctgg ttcttttgct
     1681 tggtccgcaa tccgctcaaa ttctgcaatt acttgattta tttcaattcg agcatccgtc
     1741 tcctcattca ttaaatatgg atcgccagcg actttgacac cattcttgcg aatatcaaat
     1801 tttaaccgtc gcaattgttc ttctaattct gggtctgact gaatctttcc aatcacccaa
     1861 taaatttctc caatcaactc atcataaacc atgtacatcc aactctttta atcttctggc
     1921 aatatcaaaa atgaccggaa ctgttacaga gtttccggct tgcttataca actgactatc
     1981 tgaattgact tgagaatctc gatcaaaagc ccaatcggga aagccctgaa gtcgccaaca
     2041 ctctctaggt gttagcttcc gaatttgata ctgatccgtg acgatcgctt gttggtctcc
     2101 tgttagtaag gtatgaacac tctgttttcc cagacgacct cggcgacttg tggaattagg
     2161 aaaagccaga ttcacactgt cttctggctt cacctcttga tattctgttt cattttttcg
     2221 aatcatgatt tttgtaccac cttgtttcgt tgacaaggtg ggggcggttc cagtaacttc
     2281 ataaactcga ttaccttgtt caaactttcc cggtaatagt cctgctacag agattccatc
     2341 ccttgctggc agtgcgattt tgattccttc atcacctgtt aaggttggcg ctacgccatc
     2401 tgaaccatag acttgtccac ccattcctcg attggaagga ttgatattta ccaaatttct
     2461 gatgttttga tcagatgttg aagacggtcc aatgatagga aatacttgtt cggtacatgc
     2521 gtctcgagaa tgtccgataa ggaatatccg ctcccggctt tggggaatgt aggcctttga
     2581 gttaagcacc taccactcca cgtcataccc caatccatcc agcgttcgga ggatcgtctc
     2641 gaacgtagcc cctccttcgt gattgagcaa tcctcggacg ttctcaagga ataaaagctt
     2701 aggtcggaga agagtggtga accgagcgat ttcaaagaat aaagtacctc gagtatcgga
     2761 aaatctttgc cgctttcccg caattgaaaa agcttggcac ggaaatccgc cgcaaaggat
     2821 gtccactggt ccgagggatt gaataaactc atctgataca cttgtgatgt catgcatttc
     2881 cacctcctta cttgtatcat gaattgcttt ataactacgt ctagcaaatt tatcaatttc
     2941 acaaaaaccg atacaatgat ggctcgcctg ttccatacct aaacgaaagc caccaatacc
     3001 ggcgaataaa tctaaaaatc tcatacatta cctcattcat tgcaaagaaa aaagccaaga
     3061 acattttgct cttggctcat acattat
//
LOCUS       DAJSBL010000137         2948 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.142, whole genome shotgun sequence.
ACCESSION   DAJSBL010000137 DAJSBL010000000
VERSION     DAJSBL010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2948
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.142"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..1350
                     /locus_tag="ONT85_002883"
     CDS             <1..1350
                     /locus_tag="ONT85_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HCR2379797.1"
                     /translation="TNLPALPEGTTVTDVTPGGTIDTNTPGNYEGVIEVTYPDGTKDT
                     VKVPVEVTDNRSDADKYEPTVEGEKVEIGGTVDLTDNVTNLPALPEGTTVTDVTPGGT
                     IDTNTPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSNADKYNPEGQKVTTDLNKEPDA
                     SEGIKNKEDLPKGTTYAWKEKVDVSTAGNKKGTIVVTYPDGSSDEVEVDVTVTDTRSD
                     ADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYTWKEKVDVSTAGNKKGTVVVT
                     YPDGSKEEVEVTISVVDKKAPNKPQVDPITEGDQIVTGKTEPNAEVTVTLPDGSQHHG
                     TADKNGNFTVKVPKLEAGTKVIVTATDESGNTSEPTNVVVSSNKKDSGKNGSKGSKTD
                     NQGSNSNQDKNRGKSQSSKVFPKTGESDSNIFTISGGLILLGTLGLLGYKNRKKENE"
     gene            1484..2827
                     /locus_tag="ONT85_002884"
     CDS             1484..2827
                     /locus_tag="ONT85_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCR2379798.1"
                     /translation="MEKIVIIGSNHSGLAAARFLNLSDSNYEVILIDKKSEISYLECG
                     TSLFIKGEVSNLEDLFYQNRMTLNSEIKKLFLETEVDKIDIQNKKISCVKKDGTRFSQ
                     EYDKVILATGSKQIMLDIPGNNLANIFSIKNFPNAKEIFKALDDSTIKKVGIIGAGYI
                     GVEIAEAIRKRGKEVYLFDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYL
                     GRKKIEKIVTTSGTQYSMDMVVQAIGFLSNSPIGEKELLRDVSGAYLTNEYQQTNIKD
                     IYAIGDCATTFFTSINRSSVDFSISNALRTAYIATQHINNQDFTPSGSQFTNGFSIFN
                     YNFYAAGLNLKTARLLSESIDYIEIEDFIRPAFLRKNSRVKLRISFEKNTKRIVGVQL
                     CSQEDLSGLLSMFSLAIEEQITLDKLKMLDYLFHPSFSQPYNFILKAVTGLNIET"
BASE COUNT         1171 a          398 c          561 g          818 t
ORIGIN      
        1 actaacttgc cagcgttacc agaaggaaca acagtaacag atgttactcc cggcggtaca
       61 attgatacca acacaccagg taattacgaa ggtgtcattg aagtaacgta tccagacggt
      121 acgaaagata cggtgaaagt tccagtagaa gtgacagaca atcgttctga cgccgataaa
      181 tatgaaccaa cagtagaagg cgaaaaagta gaaattggag gcacagtaga tttaacagat
      241 aatgttacta acttgccagc gttaccagaa ggaacaacag taacagatgt tactcccggc
      301 ggtacaattg ataccaacac accaggcaat tatgaaggtg tcattgaagt aacgtatcca
      361 gacggtacga aagatacggt gaaagttcca gtagaagtga cagacaatcg ttctaatgca
      421 gataaataca atcctgaagg acaaaaagtg actacagact taaataaaga gccagatgca
      481 tctgagggaa ttaagaacaa agaagattta ccaaaaggaa ctacttatgc ttggaaagag
      541 aaagtagatg ttagcacagc tggaaataaa aaaggtacta ttgtagtgac atatccagat
      601 ggatcatctg atgaagttga agtagacgtt acagtgacag atactcgttc cgatgcagat
      661 aaatataatc ctgaaggaca aaaagtgact acagacttaa ataaagagcc agatgcatct
      721 gagggaatta agaacaaaga agatctacca aaaggaacta cttatacttg gaaagaaaaa
      781 gtagatgtta gtacagctgg aaacaaaaaa ggtactgtag tagtaacata tccagatgga
      841 tctaaagaag aagtagaagt tactatttct gtagtagata aaaaagcacc gaataaacct
      901 caagttgatc ctattacaga gggtgaccaa attgttactg gtaaaactga accaaatgca
      961 gaggtgacag ttacattacc agacggaagt caacaccatg ggacagctga taaaaatggt
     1021 aattttacag ttaaagttcc taaattagag gcagggacaa aagtgatagt aactgcaact
     1081 gatgaatctg gtaatactag tgaacctact aatgtagttg tttcttccaa caaaaaagac
     1141 agtggaaaaa acggtagtaa aggtagtaag actgacaatc aaggtagcaa cagcaatcaa
     1201 gacaaaaata gaggcaaaag ccagtcctca aaagtttttc ctaagacggg agagagtgac
     1261 agtaatatct ttactattag cggtggttta atactgttag gaacgttagg attattaggg
     1321 tataaaaatc gaaaaaaaga gaatgaatag aaaatcaatt tcttaagtct tctctgagaa
     1381 gaggaagaat ctttttcttc ctctttttgc ttttttatga tgggaaataa gtgttagtta
     1441 aagatgaata tactttttat agaaaggaaa agaatgtaat taagtggaaa aaatagtaat
     1501 tattggttca aaccattcag ggctagccgc agctcgattt ctaaacttaa gcgattcaaa
     1561 ttatgaagtt attctaatcg ataaaaaatc agaaattagt tacttggaat gtgggacatc
     1621 gttatttata aaaggagaag taagtaattt agaagatctt ttttatcaaa atagaatgac
     1681 actaaattct gagataaaaa agttatttct agagacagaa gttgacaaaa ttgatattca
     1741 aaataaaaag attagttgtg taaaaaaaga tggtacaaga ttctctcaag aatatgacaa
     1801 agttatatta gctacaggtt caaagcaaat tatgctagat ataccgggga ataatttagc
     1861 taatattttt tcgataaaga attttccaaa tgcaaaagag atttttaaag ctttggatga
     1921 tagtactatt aaaaaagtag gaattattgg agcaggatat attggagttg aaattgctga
     1981 agccataaga aaaagaggga aagaagtata tcttttcgat gttgcagata gagtgttgtc
     2041 gacttattac gaccgtagtt tttcagataa agttgaagaa atactatcaa aaaatggaat
     2101 acatctttgt ctatcagaaa gtcctaaaaa atatttagga agaaaaaaga ttgaaaaaat
     2161 agtcactaca agtggtaccc aatactctat ggatatggtc gtacaagcta taggatttct
     2221 atctaatagt ccaattgggg aaaaggaact cttgagagat gttagtggag catatctaac
     2281 taatgaatac cagcaaacaa atataaaaga tatatatgct attggggatt gtgctacaac
     2341 tttttttacg tccattaata gaagtagtgt tgatttttct atttctaatg ctctaagaac
     2401 agcttatatt gcaactcaac atattaacaa tcaagatttt acaccttctg gttcccaatt
     2461 tacaaatggc ttttctatat ttaattacaa tttttacgca gcaggtttga atttaaaaac
     2521 agcacgatta ttaagtgagt ctatagatta tatagaaata gaagatttca ttagacctgc
     2581 ttttttgaga aaaaatagtc gtgtaaaatt aagaattagc tttgaaaaaa atacaaagag
     2641 aatagtaggt gtacaacttt gtagtcaaga agatttatct ggactattat caatgttttc
     2701 tcttgctata gaagaacaaa ttacattaga caaattgaaa atgttagatt atttatttca
     2761 tcctagtttt agccaaccct ataattttat tttaaaggca gtaacaggat taaacattga
     2821 aacataatcg ttactaaatg aaatcaagaa atatactgta ttactcaatt cattgaaaat
     2881 atttatcata atttttaata gaaaacacta ttaaagagaa aaaagagagg taaatcaaat
     2941 gaaaaaat
//
LOCUS       DAJSBL010000138         2837 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.143, whole genome shotgun sequence.
ACCESSION   DAJSBL010000138 DAJSBL010000000
VERSION     DAJSBL010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2837
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.143"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            798..1052
                     /locus_tag="ONT85_002885"
                     /pseudo
     CDS             798..1052
                     /locus_tag="ONT85_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405927.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
     gene            1122..1634
                     /locus_tag="ONT85_002886"
     CDS             1122..1634
                     /locus_tag="ONT85_002886"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR2379799.1"
                     /translation="MTNKNNERYSTELKATAIKKMMPPENRGIRQLSQTLGIPEATLY
                     SWRKKARIQGNTTPGTKKNVEKWSSEDKFLIVMESFSMNQVELAEYCRKKGLFKEQIE
                     AWKKTCLSANEQEENRTRELATELKEEKKQARQLEKELRHKEKALAEAAALLLLRKKA
                     HAIWGDQEEE"
     gene            1631..2668
                     /locus_tag="ONT85_002887"
     CDS             1631..2668
                     /locus_tag="ONT85_002887"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCR2379800.1"
                     /translation="MISSSDRKQAVELIQEANRNGARLTYACNELNISVRTYERWTRE
                     GTIAHDQRPLAKRPVPKNKLTDQEREKIIETVSKKEFMNLPPSQIVPKLADCSIYIAS
                     ESSFYRVLREKNMQHHRGRSQVSQKRIPPSHLATKPNEVWTWDITWLKGPIKGLFYRL
                     YLIIDLFSRKMVGWEIWESEEARHAEKLIKQTVLKERIQGQPLVLHSDNGSPMKAATF
                     QVLLEKLGIQSSYSRPRVSNDNPYSEAAFRTLKYRPEYPNNGFKTIDDARKWTKEFVD
                     WYNDSHLHSGIQFVSPAACHEGRHLAILEKRKQVYEDAKKRHPERWSKGVRNWTPHQS
                     VALNPLKETES"
BASE COUNT         1024 a          501 c          566 g          746 t
ORIGIN      
        1 ttgatgacca cgaaagtgca tggcgatatt tgatgttgat actttatgca gtaccctatg
       61 tattcttcgc tatcattctg tggctcatag cattgttttt tagagtattt aatgaccttt
      121 aggtagattt atatataaaa agggctaaat acgcaaaata cccccttaaa atgcaaaata
      181 aatgcaaata agggggtaac tccttatgaa aaaacaacta atagtattat aacacgtttt
      241 tatgccgaga aaacttattg gttggaatgg gctatgtgtt agctaacttg ttagcgagtt
      301 ggttggactt gaattgggat taatcccaag aaagtaccaa ctcaacaaca cataaagccc
      361 tgtaggttcc gaccaataag gaaattggaa taaagcaacg aaaggagttg agtaaaatga
      421 gtgagaaaac gaagaaaatc attcgagtat cattagcttt ttcacaagta gtagtgcaat
      481 cattgatagt ttattatact taccagcaat ataaattatc taaagagaat taagttttga
      541 ttatgtaata agttttggta ctaaaaaaga cttgatctaa ttagaccaag tcttttgtta
      601 gtgttatatt aataacaaaa taaaaataag aagtcgctca ctccccgacc aaagtttgtg
      661 agcaaaataa acagtcgatg tttgtttctt aattgtatat cttgatatta aacgatttaa
      721 gattattctt caagatatat attcgcaaat gtaattggaa aagcgacttc ttaaacaaaa
      781 ttaaggagtc gctttttatg gcaaaaataa cgaaaaatga gcaagaaaat ttggtggaaa
      841 attacacaaa aaaaccggcg tactctaata gccggttaga cgcacatttc gtgtgcactt
      901 ctaatccgaa attgagcttt gacgctatga cgatagtcgg aaaccttaat aaaaacagcg
      961 ctaaaaagct atccgatttt atgagtttag atccacaaat tagactttgg gatatacttc
     1021 aaacaaaatt taaagctaaa gcactacaag accccctatg ttaggctagt tgtcgtatag
     1081 gataataagt tataataaag actatacgga ggatgattac catgacaaat aaaaacaatg
     1141 aacgttactc taccgaactg aaagcgacag ccatcaagaa aatgatgcca cccgaaaata
     1201 gggggattcg ccaattaagt caaactttgg gcattccaga agcgactctt tattcttggc
     1261 gtaaaaaagc tagaattcaa gggaatacta cacccggaac gaaaaaaaat gtcgaaaaat
     1321 ggtcaagtga agataaattt ttaattgtga tggaatcatt ctcaatgaac caagtagaac
     1381 ttgcggaata ttgccgaaag aagggcttat ttaaagaaca aattgaggct tggaagaaaa
     1441 cgtgcttatc tgcgaatgaa caggaagaaa atcgaacgag agaacttgca actgaactaa
     1501 aagaagagaa aaaacaagcg cgtcaattag agaaagaatt acgtcataag gaaaaagcat
     1561 tagcggaagc cgctgctctc ttattactaa gaaaaaaagc acacgcgatt tggggggacc
     1621 aagaggaaga atgatttcat cttcagatcg caaacaagct gttgaactta ttcaagaagc
     1681 caatcgcaat ggggcccgtc tcacctatgc ctgtaatgag ctgaatatta gcgttcgaac
     1741 ctacgaacga tggacgcgtg aaggtacgat agcgcatgat caacgccctt tggcgaaacg
     1801 tcctgtaccc aaaaataaac tgacggatca agaacgcgaa aaaataatag aaaccgtttc
     1861 aaaaaaagaa tttatgaatt tgccacctag ccaaattgta ccaaaacttg cggattgctc
     1921 gatttatatt gcatcagagt ctagcttcta tcgtgtattg cgagaaaaaa atatgcaaca
     1981 tcaccgtggc cgaagtcaag tttcacaaaa acgcataccg ccaagtcacc ttgcgacgaa
     2041 gccaaatgaa gtttggactt gggatattac ttggctaaaa ggtccaataa aaggactctt
     2101 ttaccggtta tatctcatca ttgatttatt tagccgtaaa atggtaggct gggagatttg
     2161 ggaatcagaa gaagctcgtc atgccgaaaa acttattaaa cagacggtgc ttaaggaacg
     2221 tatccaagga cagccgttgg ttttacactc cgataatggc agccctatga aagccgcgac
     2281 ttttcaagtc ttactcgaaa aattaggaat tcaaagttct tattcaagac cacgagtgag
     2341 taatgacaat ccttattcgg aagcggcttt ccgaacgtta aaatatcgac cagaatatcc
     2401 caataacggc tttaaaacaa tagatgatgc tcgaaaatgg acgaaagagt ttgtcgattg
     2461 gtataacgat tctcatttgc atagtgggat tcaatttgtg tctcctgcag cgtgtcatga
     2521 aggacgacat ctcgctattt tagagaaacg aaagcaggtt tacgaggacg ctaaaaagcg
     2581 ccatcctgaa cgctggtcaa aaggtgttcg aaattggact ccacatcaat cggtggcgtt
     2641 gaatccttta aaagaaacag aaagttaaca ggagcataat ctttcaagat tatcaaataa
     2701 ccctcgtcac ccaatagggg aaaaagttat tcttccccta ttggataacg aggccaaccg
     2761 accataggga ggttctgctt tttgataggt ggaaaataaa aatacgacaa ctatattgac
     2821 aaacaccgac catcgtc
//
LOCUS       DAJSBL010000139         2772 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.144, whole genome shotgun sequence.
ACCESSION   DAJSBL010000139 DAJSBL010000000
VERSION     DAJSBL010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2772
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.144"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            175..519
                     /locus_tag="ONT85_002888"
     CDS             175..519
                     /locus_tag="ONT85_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HCR2379801.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            520..939
                     /locus_tag="ONT85_002889"
     CDS             520..939
                     /locus_tag="ONT85_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HCR2379802.1"
                     /translation="MTTKHEEIHNGVIFPAGEKNEAYAQVFTGQSYLNMLVNDPEVSV
                     GVGNVIFEPGCRNHWHVHKDGYQILLVTGGEGWYQEEGKPARRLMAGDVVVTHKGIKH
                     WHGATKDSWFEHLAITAGNSEFLEPVTDEVYEGLENQ"
     gene            complement(1031..1381)
                     /locus_tag="ONT85_002890"
     CDS             complement(1031..1381)
                     /locus_tag="ONT85_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2379803.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1347..2567)
                     /locus_tag="ONT85_002891"
     CDS             complement(1347..2567)
                     /locus_tag="ONT85_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HCR2379804.1"
                     /translation="MLSNTPTDNWSGLLTILTSPSNLLTDYFALGGFGSAFINVGILT
                     LLSVLLAYRHKVILNGPLFASILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQ
                     YIMIGLFGSALSPVVSYITFGMRFPLLIGILLGNLAGIAIGLLLPPLAAQTLVFHRGF
                     TLYNIGFTSGLIAMTFTAVLRLFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFY
                     YNSFRLLGIREIFDSSGKLTTDFIANSGIGATLINIGLVGLMLSSYVLLVGGQLNGPV
                     IGAILSAVGFSAFGCHLKNSFPILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPIS
                     GFYGSFYGVIAGVLHIALVHNVSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKE
                     KKTLGKRIIKKNHR"
BASE COUNT         1031 a          499 c          534 g          708 t
ORIGIN      
        1 aaaataaaaa aagcgttagc aactatgctt aaaaagcata tcgactgaat aaactaagca
       61 ttggacaggc tgaaagacta catttaaaat gaaatgcgaa gaaacaatag aaaataaagt
      121 tgccttagaa ccatagttga atcacctaac agtcgtgaag ggagaataag agaaatggca
      181 gaaaaacaaa ccgcaggacg agatcaatta ggagattttg caccaaaatt tgcagaattg
      241 aatgatgatg ttttatttgg acaagtatgg tccagagaag aattatctgc tcatcagcgt
      301 agtatcatca ccatatctgc attaatttca ggaggaaatt ttgaacaatt gccagctcat
      361 ctgaagattg gcaagcaaaa tggcattact agtgaagaaa tagcagaaat cattacccat
      421 ttagcttttt atgtaggatg gccaaaagct tggtcagctt ttagtttagc aaaagaaatc
      481 tatttagaag aaaatagcga agataagaaa gaggcgtaaa tgacgacaaa acacgaagaa
      541 atacacaatg gcgtgatttt tccagcaggt gaaaaaaacg aagcttacgc tcaagtattt
      601 accgggcaaa gctatctgaa tatgttagtg aatgatcccg aagtttctgt tggtgtaggt
      661 aatgtaattt ttgaaccagg ttgtcgcaat cactggcatg tccataaaga cggctatcaa
      721 atcttacttg tgaccggagg agaaggctgg tatcaggaag aaggaaagcc agcaagacga
      781 ttaatggctg gagatgtcgt cgtaacgcac aaagggatta aacattggca tggtgcgaca
      841 aaagacagtt ggtttgaaca tttggcgatc actgctggta attcagagtt tttagaacct
      901 gtaacagatg aagtgtatga aggattggaa aatcaataat gtcaagcttg aacagctcct
      961 gaaagtagat tttaatcgaa ctttcaggag ctgctttttt gattcagtct tttataaata
     1021 gaaaataggg ttaataatag gaatgagaag cacgtcgaaa gatcttaata ttgataatcg
     1081 gctgggcata catgatttcc acgtcatcaa tcaaactacc taacagatga taatcttccc
     1141 gatcatgtcg gggattacct aatagttctt gatagtagat ttccatacta gggtcccgag
     1201 gattgttcag caaatcagca agtacatcta aatcttctgg ctgagtttct gccgtaccct
     1261 gtatctctat gaaaagccct tcctcagcaa attctactcc aatacgtatt tcagttattt
     1321 catgggaata taaataagta agaagctcat cgatgattct ttttgataat tctttttcca
     1381 agtgtctttt tttcctttct gacagcggta aagttatcta gtatcggtac aaggatacct
     1441 gcaacgaaac cagttgaaaa accactgtta tatagattta gtcctccgtg caaagtgctg
     1501 acgttatgga caagagcaat atgtagtaca ccagctatta caccgtaaaa actgccgtaa
     1561 aaaccgctta tgggcgcgag acctgtccca aacacagctg caacaagcat gccagtactg
     1621 gtgatctcat gaaaagttcc aaacaaagaa gcaataaata ttccgactag tatcggaaaa
     1681 ctgtttttta aatgacaacc aaaagcactg aaacccaccg cagataaaat agcaccgatg
     1741 acaggtccat ttaactgacc gccaaccagc aaaacataag aagacagcat cagaccaact
     1801 aagcctatgt tgatcaaagt agcaccaata ccagaattag caatgaaatc agttgtcaat
     1861 tttccagatg aatcgaaaat ttctcgaatc cccaaaagcc gaaaggaatt ataatagaaa
     1921 ccaattccga ctgtcagcga aaaaaagcca aaaataatcc aaatgagagg aaagtgatat
     1981 tcattgaaaa ccaatgtatt ttcgacgatt gaatagctga ataacctcaa aacagcagta
     2041 aaagtcatcg caatcaaccc agaggtgaat ccaatattat aaagtgtgaa tccacgatgg
     2101 aaaactaaag tttgagcagc taagggcgga agaagtagac caattgctat gccagctaaa
     2161 ttccccaata aaatacctat cagtaaagga aaacgcatac caaaagtgat ataactaacg
     2221 accggtgata atgcgctacc aaacagtccg atcatgatgt actgagaaaa aggtttattg
     2281 acaaatacag cgtatagata cacacctaaa atgattgaga tagaattata aaggtttttg
     2341 ccaaaaaaag agaaaccggt aactgttaga atcgaagcaa ataagggacc gtttaaaatc
     2401 accttgtgcc gataagctaa aagcacagaa agtaaggtca aaatccctac atttatgaat
     2461 gcgcttccga aaccgcctaa agcaaaataa tctgtcaaaa gattgcttgg cgaggtaaga
     2521 atagttagca atccactcca attatcagta ggagtattag aaagcaagcc aataaaaaga
     2581 aaaaaacacc tgtaaaacca agataaaaat actttctttt ctgtgaaacg attcgttttt
     2641 gaggatgaat ataaataacg tcagtttcgt tttccattac acaccttctt tttcattttc
     2701 tttaactata acttgaaatg aaagatttgg acaactatca tttaggtcaa aactaaaaat
     2761 gtaagggaac cc
//
LOCUS       DAJSBL010000140         2723 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.145, whole genome shotgun sequence.
ACCESSION   DAJSBL010000140 DAJSBL010000000
VERSION     DAJSBL010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2723
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.145"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            700..2610
                     /gene="ltrA"
                     /locus_tag="ONT85_002892"
     CDS             700..2610
                     /gene="ltrA"
                     /locus_tag="ONT85_002892"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HCR2379805.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
BASE COUNT         1067 a          420 c          558 g          678 t
ORIGIN      
        1 aaaagctaag gcactaataa aagaacgaaa aagtttggtt attaggagaa ctgataatct
       61 gacaggtgga atgatgaagt aacgctctga aacgcctgcc ctgagcctcc gacaagcatt
      121 gtatgaggaa actcagaatg tttgaagctc ggtaaagacg gctgaaagat accgtaatag
      181 ttgatgaaca tatatcttct atcgaaaact atccagaggt gggtagcgta tccaacaggt
      241 cgggggtcta taaaatatct atggttagaa tgttctataa ggaactgacg aaacagcgaa
      301 tgtacgggtc taaatttgaa ctttgtagaa atgcaaaggt acgagaaatc gtagctggtg
      361 tggcaaagta agaatatagg ttatgaaatg ccatataacg ttacaggcgg tatcaagcca
      421 gcaggcttat agctagtgac ctaaggatat atatgtatag ataagattat cggaacgagg
      481 aaaggtattg ggttctcatc aagagaataa actgacgaag aacaataagc agttgaacta
      541 atgctgaaaa gcagagggcg aacctaagaa attcttgtaa tgagaatgga ggaatgccct
      601 caagtcgtgt atatattaga aaagtattat gttgtttcaa taaggattac gagtaagata
      661 aaaaagctca ctcaatttca agtaaaggag tgaagcctta tgcctaaaaa taaaaatgaa
      721 acattgtgtg tagaagattt aagacacgct gaatattacg aaatgcaaaa cacctttgat
      781 gatttatatg ccaaaagtaa gaatggagat attttcactc atcttatgga tattatccta
      841 tcgagagaaa atatattact ggcatatcga aatatcaaag caaatgcagg cagtaaaaca
      901 gcaggaacag acggtacaat aattaaagat attggcaaat tgccagcaga aacagttgtc
      961 aaaaaagtaa gatacattgt tgcaggcagt ccacatgggt atcgacctaa gccagtgagg
     1021 cgaaaagaaa taccaaaacc aaatggtaaa accagacctc taggtattcc ttgtatgtgg
     1081 gacaggctta tacaacaatg tataaaacaa gtattagaac caatctgcga agctaaattt
     1141 agtgaaaaca gctatggttt tagacctaat aggtcagttg aaaatgcaat aaaagcaacc
     1201 tataaccgcc tacaaatcag ccagctacat tatgtgattg aatttgatat taagggtttc
     1261 tttgacaacg tgaaccattc caagctgata aaacagatat gggctatggg aatacgggac
     1321 aagcacttga tattcatttt aaaaaggata ttaaaagctc caatcaaaat gactaacggt
     1381 acaataacat atccagagaa aggaactccg caagggggaa taatatcccc actactagca
     1441 aacattgttc ttaatgagtt agaccattgg gtagaaagcc aatggcagga aaatcctgtt
     1501 acaaaaaact atgtggttca tatcaataaa agcggtagcc catgtaaaag caacgcttac
     1561 aaagaaatga agaaaacaaa gttaaaggaa atgtatatgg ttcgttatgc agatgatttt
     1621 agagttttct gccgctacaa ggaaagtgct gaaaaagcca aaatagcaat cactcaatgg
     1681 attgaacaac gattaaaatt ggaagtatca caagaaaaaa caagaattgt aaatgttaga
     1741 aaaagatact ccgactttct tggtttcaag attaaaatga taccgagaag aaagaagtta
     1801 gttgttaaat cacatataag tgataaacaa ttcaaaaacc aaaaagacaa gctggtatca
     1861 caggcaaaga aaattgcttc tccctcaaaa gggaaaacgg aattagaaga aacaagactt
     1921 tataattcaa tggtattagg tatgcaaaat tattatcaga ttgcaacgaa tgtaaatctg
     1981 gattgtatga aactgaatag agctgtaatg gctatcttca ctaacagatt aggtacaaac
     2041 aaaaggggca gattgagaaa aacaggcaga caacttacga caaaggaggc aacaagatat
     2101 ggtaaatcta aaatgcttcg gtatgttgct ggcgagggcg aacctatttt ccctgttgga
     2161 tatatccaac acagaaaacc aatggcaaag gtgtatttag cgaattgcta cactctagag
     2221 ggaagaaaat ttatccacac caatctaagt gttgataaat tactgatgta ccagttaatg
     2281 aaattatctt tggaaaatcg cacaatagaa tacgcagaca atcgaatttc gctattttct
     2341 gctcaacatg gaaaatgtgc tataactctg gaagaatttc aacaagctaa tgaaatacat
     2401 tgtcatcata ttgtaccaac tggagctggt ggaaatgacg attataaaaa tcttattttg
     2461 gtaaaagagg cggtacaccg tctaatacac gctaaaacag aagaaacaat acaaaaatat
     2521 atagctctat tgaagctaaa taaagtccag ctcataagac taaataaata tagagtatta
     2581 gctggcaatc aagaattaaa cttgatatag gtctactaca taaatacttt tcaatataaa
     2641 cacgatggaa cgcggagtgc ggtgaaagtc gcacgctccg tgtgaatggg gggaaaagtt
     2701 tgagatattt ataatatcag gaa
//
LOCUS       DAJSBL010000141         2625 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.146, whole genome shotgun sequence.
ACCESSION   DAJSBL010000141 DAJSBL010000000
VERSION     DAJSBL010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2625
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.146"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(61..936)
                     /locus_tag="ONT85_002893"
     CDS             complement(61..936)
                     /locus_tag="ONT85_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCR2379806.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            complement(1191..1275)
                     /locus_tag="ONT85_002894"
     tRNA            complement(1191..1275)
                     /locus_tag="ONT85_002894"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1239..1241),aa:Leu,seq:caa)
     gene            complement(1293..1364)
                     /locus_tag="ONT85_002895"
     tRNA            complement(1293..1364)
                     /locus_tag="ONT85_002895"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1330..1332),aa:Gln,seq:ttg)
     gene            complement(1374..1446)
                     /locus_tag="ONT85_002896"
     tRNA            complement(1374..1446)
                     /locus_tag="ONT85_002896"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1411..1413),aa:His,seq:gtg)
     gene            complement(1508..1592)
                     /locus_tag="ONT85_002897"
     tRNA            complement(1508..1592)
                     /locus_tag="ONT85_002897"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1556..1558),aa:Leu,seq:caa)
     gene            complement(1613..1683)
                     /locus_tag="ONT85_002898"
     tRNA            complement(1613..1683)
                     /locus_tag="ONT85_002898"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1649..1651),aa:Cys,seq:gca)
     gene            complement(1729..1800)
                     /locus_tag="ONT85_002899"
     tRNA            complement(1729..1800)
                     /locus_tag="ONT85_002899"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1766..1768),aa:Gln,seq:ttg)
     gene            complement(1812..1884)
                     /locus_tag="ONT85_002900"
     tRNA            complement(1812..1884)
                     /locus_tag="ONT85_002900"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1849..1851),aa:His,seq:gtg)
     gene            complement(1887..1960)
                     /locus_tag="ONT85_002901"
     tRNA            complement(1887..1960)
                     /locus_tag="ONT85_002901"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1926..1928),aa:Trp,seq:cca)
     gene            complement(1967..2047)
                     /locus_tag="ONT85_002902"
     tRNA            complement(1967..2047)
                     /locus_tag="ONT85_002902"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2011..2013),aa:Tyr,seq:gta)
     gene            complement(2049..2124)
                     /locus_tag="ONT85_002903"
     tRNA            complement(2049..2124)
                     /locus_tag="ONT85_002903"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2089..2091),aa:Phe,seq:gaa)
     gene            complement(2160..2232)
                     /locus_tag="ONT85_002904"
     tRNA            complement(2160..2232)
                     /locus_tag="ONT85_002904"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2197..2199),aa:Asp,seq:gtc)
     gene            complement(2242..2314)
                     /locus_tag="ONT85_002905"
     tRNA            complement(2242..2314)
                     /locus_tag="ONT85_002905"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2279..2281),aa:Val,seq:tac)
     gene            complement(2330..2401)
                     /locus_tag="ONT85_002906"
     tRNA            complement(2330..2401)
                     /locus_tag="ONT85_002906"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2366..2368),aa:Glu,seq:ttc)
     gene            complement(2416..2505)
                     /locus_tag="ONT85_002907"
     tRNA            complement(2416..2505)
                     /locus_tag="ONT85_002907"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2469..2471),aa:Ser,seq:gga)
     gene            complement(2545..2618)
                     /locus_tag="ONT85_002908"
     tRNA            complement(2545..2618)
                     /locus_tag="ONT85_002908"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2582..2584),aa:Asn,seq:gtt)
BASE COUNT          784 a          604 c          496 g          741 t
ORIGIN      
        1 aatcatagtc catttactat gtgtcatttg gttgcgccta tagatagtta cgcccttatt
       61 ttaatagatt cttgctagat gaattgccaa atagatgatt tcattttctt gaatggcaag
      121 ttgaaattgc tgttcgatga tatctttaat ctttagtgct gtacaatacg ctttgctatg
      181 tttaaatgtt tcagtaaaaa aaccaatatt ttctactggc gtttcttcct gataaatcaa
      241 gcgtttaacc aggaatttga tatgaaccat taagcgatga taagtcagtg attctttatc
      301 ataaacgttt gaataggatt tttctagatg ggacaaaatc gtggtcgtca tttctactat
      361 ttttaagctc tttaaactat taggctctcc tagctcagca ttaatgatat gcatagtgat
      421 gaatcctact tcatttgacc ctaactcggt ttgatatcga tgattgattc gctcgaccac
      481 ttctactgct atatcatatt catttggata aaggaatctc atttctggca acataattgc
      541 tgtgacatct ctatcttctc tttttcgttg aatagcaaaa taaatatgat ctgtcaatgt
      601 aatatagata tttgaatcta atggagagga gatcctagct gttatcagtt caatcacttc
      661 ttccgcaaaa tccataaatt cgattggaat ctgcgagact atctcactga atcgtttgat
      721 cttttcttta gaatctaata caaataattt gtcagcatcc tcaacatcaa atggcttact
      781 cactttagca gaaaagccaa tccctttccc catcgcaatt ttttctattc catcgtcatc
      841 gatcaataca gcattatgat tcagcttttt catcacatac accgttaatt cacctcctct
      901 atatgataaa acaaaacaac ttgcagactc cttcatacat atttttgtct ccaaacaatt
      961 tttaaagaca aaaaacgacc taagaaagaa ccctttctta ggtcgtgcct aattgaatag
     1021 tagcacccta cataatttac aaaaatagtt taacacataa taaaagcgtt atcaatatct
     1081 tttttactcg ttttatgata atacttgtat cgaattgatt tggaggtaat tacataaaaa
     1141 caaaaaaaat atagcaactc tctataagaa agttgctata ttgataatta taccggcggc
     1201 cggggtcgaa ccggcacgtc ctcaacggac actggatttt gagtccagcg cgtctgccaa
     1261 ttccgccacg ccggcaaatt taaaaataat aactggggta gctggattcg aaccaacgca
     1321 tgagggagtc aaagtccctt gccttaccgc ttggctatac cccaagaata aagggcgaat
     1381 gatgggaatc gaacccacga atgccggagc cacaatccgg tgcgttaacc acttcgccac
     1441 attcgccatc ataaaagatg ttatttaatt tctcaataaa aaactaagat gaagacatct
     1501 tagtcaatac cggcggccgg ggtcgaaccg gcacgccctc aacgggcact ggattttgag
     1561 tccagcgcgt ctgccaattc cgccacgccg gcaagttgat ttattagtac taaggcggta
     1621 accggatttg aaccggtgat gaaggttttg cagacctctg ccttaccact tggctatacc
     1681 gccattatac tattatcaat ctataaaata aagaataaat tatagaaact ggggtagctg
     1741 gattcgaacc aacgcatgag ggagtcaaag tcccttgcct taccgcttgg ctatacccca
     1801 agaataaaaa gggcgaatga tgggaatcga acccacgaat gccggagcca caatccggtg
     1861 cgttaaccac ttcgccacat tcgccatggc aggggcagca ggaattgaac ccacactaac
     1921 ggttttggag accgctgttc tacctttaaa ctatgcccct ataaaatgga ggagagtgga
     1981 ttcgaaccac tgaaccctaa ggaacggatt tacagtccgt cgcgtttagc cacttcgcta
     2041 ctcctccatg gtggcgcggg acagaatcga actgccgaca cacggagctt caatccgttg
     2101 ctctaccaac tgagctaccg cgccatattt taaaattatt aattgaataa caagaacgac
     2161 ggttccgacg ggaatcgaac ccgcgatctc ctgcgtgaca ggcaggcatg ttaaccccta
     2221 caccacggaa ccagttcatt atggaggtta acgggatcga accgctgacc ctctgcttgt
     2281 aaggcagatg ctctcccagc tgagctaaac ctccaacatt caattaaaat gacccgtacg
     2341 ggattcgaac ccgtgttacc gccgtgaaag ggcggtgtct taaccgcttg accaacgggc
     2401 caggtaaatt tgctacggag agtaagggat tcgaaccctt gagacagttt ggcaccatct
     2461 acatgatttc caatcatgct ccttcggcct ctcggacaac tctccagagt gaaagaagct
     2521 caatactctt tctcaaaatg agaactccgg cagtaggact cgaacctacg acatcatgat
     2581 taacagtcat gcgctactac caactgagct atgccggaat aacat
//
LOCUS       DAJSBL010000142         2509 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.147, whole genome shotgun sequence.
ACCESSION   DAJSBL010000142 DAJSBL010000000
VERSION     DAJSBL010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2509
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.147"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..1211)
                     /locus_tag="ONT85_002909"
     CDS             complement(<1..1211)
                     /locus_tag="ONT85_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HCR2379807.1"
                     /translation="MSNDTRPEIRFPGFTEDWEERKLGDMMDVTSVKRIHQSDWTNSG
                     IRFLRARDIVSAAKNEEPSDYLYISEEKYNEYSKNSGKVSQGDLLVTGVGSIGVPLLI
                     TDDNPIYFKDGNIIWFKNEHKIDGNFFYYSFINNKIQKYIRDVAGIGTVGTYTIDSGK
                     KTPISLPTYDEQIKIGSFFKQLDHTIMLHQHELDLLKETKKGFLQKMFPKNGAKVPEV
                     RFPGFTGDWEERKLGEVVVFLNGRAFKQQELLDSGKYRVLRVGNFNTNDRWYYSNLEL
                     EEDKYANKGDLLYLWATSFGPEMWNEERVIYHYHIWKLKFLSKELDKQYLYTWLLTDK
                     EKIKESTNGTTMVHVTKGGIEQRTFQFPPDINEQQKIGAFFKQLDDTIALHQRKLDLL
                     KETKKGFLQKM"
     gene            complement(1201..>2509)
                     /locus_tag="ONT85_002910"
     CDS             complement(1201..>2509)
                     /locus_tag="ONT85_002910"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HCR2379808.1"
                     /translation="LFNVLADQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQS
                     KKLGTDEQQRNVTITEVIKKLNDVEVLGHDGDVIGDAYEFLISQFASEAGKKAGEFYT
                     PHMVSDMMAQIVTLDQKERRFFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTT
                     TYNLAKMNLILHGVDAEEMNLRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTF
                     LDDSRFNRYGKLAPKSKADFAFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKL
                     LEDGSIYAVIGMPANLFFGTSIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSE
                     ENIQKILETYAERKDVEKYAHLATFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGND
                     IKKIRQEQQVLEKELLEAISSLQTTPENEAWLQGALEVFKHEQ"
BASE COUNT          698 a          528 c          419 g          864 t
ORIGIN      
        1 aacatctttt gcaagaagcc ttttttcgtt tccttcagca aatctaactt acgttgatga
       61 agagcgatcg tatcatctag ctgcttgaag aaggcaccaa ttttttgttg ttcatttata
      121 tccggtggaa actgaaatgt cctttgttct ataccacctt tagtcacatg taccattgtc
      181 gttccgttag ttgattcttt aattttctct ttatcagtca atagccaagt atatagatac
      241 tgtttgtcca attccttaga taaaaattta agtttccaaa tatgataatg gtagattaca
      301 cgttcttcat tccacatttc aggaccgaag cttgttgccc ataaatataa gagatctcct
      361 ttattagcat acttgtcttc ctctaattct aagtttgaat aataccatct atcattagta
      421 ttaaaattac ctactcttaa aaccctatat tttccagaat caagtaattc ttgttgttta
      481 aaagctctac cattcaaaaa aactaccact tcccccaact tccgctcttc ccaatctccc
      541 gtaaatcctg gaaaacgaac ttctggcact tttgctccgt ttttagggaa cattttttgt
      601 aagaatcctt ttttcgtttc cttcagcaaa tctaattcgt gctggtgaag catgatggtg
      661 tgatcaagtt gtttgaaaaa tgaaccgatc ttgatctgtt cgtcataagt tggaagtgag
      721 ataggtgtct ttttaccact atcaattgta taggttccga cggttccaat gcctgcaacg
      781 tcacggatat acttctgaat tttgttattt atgaacgagt agtaaaagaa gtttccgtca
      841 attttatgct cattcttgaa ccaaatgatg tttccatcct taaagtaaat tgggttatca
      901 tcagtgatta ataaggggac acctattgat ccgacaccag ttacaagcaa gtctccttga
      961 gaaactttac ctgaattttt cgaatattca ttatattttt cttctgaaat gtaaagataa
     1021 tcggatggtt cctcattttt tgcagctgag acaatatcac gagcacgtaa aaatctaata
     1081 cctgagttag tccaatctga ttgatgaatt cgcttcacag aagtaacatc catcatatct
     1141 cctaacttac gctcttccca atcttccgta aaaccaggaa accggatttc tggtcgtgta
     1201 tcattgctca tgtttgaaca cctccagcgc accttgtaac cacgcttcat tttcaggtgt
     1261 tgtttgcagg gaagagatag cttctaaaag ttctttttct agcacttgct gttcttgtcg
     1321 tatcttcttg atgtcatttc cgacatgaac catatcaatt ggttcttctt cttcgaaagt
     1381 atcgacatag cgtgggatat ttaagttata gtcattttct ttgatttcat caaaggttgc
     1441 caaatgagcg tatttctcga catcttttct ttcagcgtag gtttcgagga ttttttgaat
     1501 gttttcttcc gataatttat tctgattttt ccctttaaca aattcacgac tagcatcaat
     1561 gaataataca tctcgagttt gacgattctt cttcaataca atcaccgtcg ttggaatcga
     1621 tgtaccaaag aacaagttcg caggcatacc gatcacagca taaatactgc cgtcttctaa
     1681 taatttttga cgaatgaccc cttctgcagc accacggaat aaaaccccgt gtggcaagac
     1741 gatagccata gtccctgttt ccttcaaatg atagaaacca tgaagaagaa acgcaaaatc
     1801 tgctttggat tttggcgcta atttcccgta gcgattaaac cgagaatcat ctaagaaggt
     1861 cgtatctgct gaccaattag ccgaataagg tggattcatc actactgcat caaatgtgta
     1921 aggttcatct gttggccagt ccttattcaa cgtatcccca ttgcggagat tcatttcttc
     1981 ggcatctacc ccatgtaaaa tcaagttcat ttttgctaga ttgtatgtcg ttgtatttag
     2041 ttcttgccca tgatatttaa cgttatctgg atgagttaag tagttacgta catttaacat
     2101 caaggaacct gaacccatgg ttgggtcaaa gacactaaag aatcgtcgtt ctttttgatc
     2161 caacgtcacg atttgtgcca tcatatcaga aaccatatga ggcgtgtaaa attctccggc
     2221 tttcttccct gcttctgaag caaattgact gatcagaaat tcatacgcat ccccgatcac
     2281 atccccatcg tgacctaaaa cttccacgtc attcaatttc ttgataactt cggtaatcgt
     2341 cacatttcgt tgttgctcat ctgttcctag tttttttgat tggagatcca catcatcaaa
     2401 taacccatta aattgattgt aggtagatgc caattgcaca aaggctttgt ttagatcgtt
     2461 caattggaac gttgcttgtt ttgcttgatc cgctaagacg ttgaaaaga
//
LOCUS       DAJSBL010000143         2454 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.148, whole genome shotgun sequence.
ACCESSION   DAJSBL010000143 DAJSBL010000000
VERSION     DAJSBL010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2454
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.148"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            132..344
                     /locus_tag="ONT85_002911"
     CDS             132..344
                     /locus_tag="ONT85_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2379809.1"
                     /translation="MAVWQIQLKERGAYGLISLSDVLCRKEMPDLFSLDQIIAIIGAA
                     AVVITAVAKASDSILAWLKFKKEQKK"
     gene            506..1024
                     /locus_tag="ONT85_002912"
     CDS             506..1024
                     /locus_tag="ONT85_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HCR2379810.1"
                     /translation="MDKFNRLTLINQFEILKALNPNEEKYYAEKIEILTEGYEYHYSE
                     IFENISDPLPEEDSRFVLDILNMYRDITFSKNKLKDINSIDNYYIQFKGFDFNSSTEF
                     KLALYAQFFIEKLNRFQEIVEDSDFNTFNSHKPMRGTYIKFLENYNDLKSTNNYQFGN
                     LSYEMISKVLSL"
     gene            1031..1543
                     /locus_tag="ONT85_002913"
     CDS             1031..1543
                     /locus_tag="ONT85_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2379811.1"
                     /translation="MKLNSINDLFAIIGVIANILFSCLNFRFTQKNKKNKLSIEQMKK
                     DIETDLKNDHKERRKIEEYTSLMEKCKATDAIFSFLRKSDEIITRENIDLENEISKLE
                     SIFSDINSLKYLIVEENSDSFLEFEKYLTDLIDNRNNELMRQNIARFLTKLVSEWKSI
                     ISQNMDKLRY"
     gene            1814..2437
                     /locus_tag="ONT85_002914"
     CDS             1814..2437
                     /locus_tag="ONT85_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR2379812.1"
                     /translation="MYLAYLRVSSDDQSLARQHKLIENWSVKNNIAEDDLKIFEEKVS
                     GKNIENRPELQELISFIRERDTIIIPSLDRLARNSKDTKDLLQLFRSKGASIEILDLP
                     SFNGVTDPALRDLLTNLVIEVFSYVAENERKKIKERQKQGIEIAKKNGRYTGKKIQYH
                     ANATGKNKLIYDEIVDGLKKKETISKIAKNAGVTRKTVYRVKRDLGM"
BASE COUNT          944 a          310 c          408 g          792 t
ORIGIN      
        1 aaaacactat atattccgaa aagtgagtat ctccattcat aaaataatca acatatttaa
       61 aaaatctaat tagctttttt agtcaatcta aagttcttta ttctatgata taatgttact
      121 gtagaaagtt tgtggcggtt tggcaaatcc aattgaagga gcgtggtgcc tatgggctta
      181 ttagcttatc ggacgtactc tgtagaaagg aaatgcctga tttgttctca ttggatcaaa
      241 tcattgctat tatcggagca gcagcagtag ttattactgc cgtagcgaaa gctagcgatt
      301 cgattttagc ttggttaaag ttcaaaaaag aacaaaaaaa atagagccgt ctcaactttt
      361 ggcgaggtag acgagctcta aagctttatt tacatgccat ccgctttgaa cggactacac
      421 tgggcatgtg acagcatgcc ctttcattta tattatatct cattctttta tataatgaaa
      481 gtaaataaat catggaggat aaaatatgga taaatttaat agattaacat taataaatca
      541 gtttgaaatt ttaaaagctt taaatcctaa cgaagaaaaa tactatgctg aaaaaataga
      601 aatactaaca gaaggttacg aatatcacta tagtgaaata tttgaaaata tttctgatcc
      661 attgccagaa gaagattcgc ggtttgttct agacatatta aatatgtata gagatattac
      721 ttttagtaaa aataaattaa aagatataaa ttcaattgat aattattata tacaatttaa
      781 aggttttgac tttaatagct ctaccgaatt taagttagca ctttatgcac aattttttat
      841 tgaaaaatta aataggtttc aagaaatagt tgaagatagt gattttaaca cttttaatag
      901 ccataaaccg atgagaggta cttatataaa atttctcgaa aattataatg atttaaaatc
      961 taccaataat tatcaatttg gaaacttgtc ttatgagatg ataagtaaag ttctaagttt
     1021 gtgatgataa atgaaattaa atagtataaa tgatttattt gctattatag gtgttattgc
     1081 taacatactt ttttcgtgtt tgaattttag atttactcaa aagaacaaaa agaacaaact
     1141 cagtatagaa caaatgaaaa aagacattga aactgattta aaaaatgacc ataaagaaag
     1201 aagaaaaatt gaagaatata cttctttaat ggaaaagtgt aaagcaactg atgcaatttt
     1261 tagcttttta agaaaaagtg atgagataat cacacgtgaa aatatagatt tagaaaatga
     1321 gattagtaaa cttgaatcta tcttttccga tataaattct cttaagtatt taatcgtcga
     1381 agaaaattcg gatagctttt tagaatttga aaaatatcta acagatctta tagataatag
     1441 aaataatgaa ttaatgagac aaaacattgc aagattcctt actaaattag tttctgaatg
     1501 gaaaagtatt atttctcaaa atatggataa attaagatat taaggatgtt agttttaaaa
     1561 taaactaatg tccttatttt tgtgttcaca tttctgttac ctaagaaata cttaaaggag
     1621 gtgatgtagc cagcaacgac tacaacttga acccgctatc tacagtttat ttgttgtttc
     1681 ttttgggtat actctaatgg tacaaatcca tatttgctta aagcgttgat ttttcagcgg
     1741 tttttcttta ttgcaaagtg taccctaaga cctgtatact aatagtaaat agattggata
     1801 ggaatgatgg caaatgtatt tagcttattt aagagtttct tccgacgatc aaagtttagc
     1861 tcggcaacat aagctaattg aaaattggtc agtcaaaaat aatattgctg aagacgattt
     1921 aaaaattttc gaggaaaaag tctctggaaa aaatattgaa aacagaccag agctgcaaga
     1981 gcttatttct ttcatacgtg aacgagatac gattattatt cctagtcttg atcgattagc
     2041 aagaaatagt aaagatacaa aagacttact tcaattgttt cgtagtaaag gtgcttctat
     2101 tgaaatatta gacctaccgt cgtttaatgg ggtaactgac cctgcattaa gagacttgct
     2161 aacaaattta gtcatagaag tgtttagcta tgttgctgaa aacgaacgga aaaaaatcaa
     2221 agagcgtcaa aaacaaggca ttgaaattgc aaagaaaaat ggtcgttata ctggtaaaaa
     2281 gattcaatac catgccaatg caacaggaaa aaacaaattg atttacgatg aaattgttga
     2341 tggtctgaag aaaaaagaaa ctatttctaa aatagcaaag aacgcaggag ttacaagaaa
     2401 aaccgtttat cgagtaaaaa gagatttagg aatgtaatat gttccagtta gaaa
//
LOCUS       DAJSBL010000144         2431 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.149, whole genome shotgun sequence.
ACCESSION   DAJSBL010000144 DAJSBL010000000
VERSION     DAJSBL010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2431
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.149"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            221..838
                     /locus_tag="ONT85_002915"
     CDS             221..838
                     /locus_tag="ONT85_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR2379813.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            879..1178
                     /locus_tag="ONT85_002916"
     CDS             879..1178
                     /locus_tag="ONT85_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BRCT domain-containing protein"
                     /protein_id="HCR2379814.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            1212..1379
                     /locus_tag="ONT85_002917"
     CDS             1212..1379
                     /locus_tag="ONT85_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR2379815.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(1424..2029)
                     /locus_tag="ONT85_002918"
     CDS             complement(1424..2029)
                     /locus_tag="ONT85_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR2379816.1"
                     /translation="MGKIGYIRVSSQDQNLDRQLAMMEGLNIDKLFQEKASGKDTERP
                     EFQKLLNYIREGDCVIVTSLDRLGRDYEDIKNTVAFMKQKKVALKILDAQFLDFNTGN
                     ELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAQKAGRYKGRPIEYSINSTDPQ
                     KRLVFKTVVDMLKQGIPVAEIAKENGISRPTIYKIKKANDL"
     gene            complement(2289..>2431)
                     /locus_tag="ONT85_002919"
     CDS             complement(2289..>2431)
                     /locus_tag="ONT85_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HCR2379817.1"
                     /translation="QSLRTASSTIKGMETIRGIYKKNRRNGTLFGFSVSTEIKVLMGI
                     TA"
BASE COUNT          787 a          407 c          457 g          780 t
ORIGIN      
        1 atggcagacc gttaaggcct gccattttta tgtattttag cggaataaat ataatcatgc
       61 gggagtgtaa acttactgtt tcaaaaagta tactttttga catgccttga ttttctttgt
      121 ttatgtatgg cataataacc acataataca atttgaaacg tttctgattt aatctgattg
      181 ttttgaaaca attttagtga aaaaagggta gataaaaacg atgagaaaat ttttttatat
      241 ccgggtcagt agttatgatc aatcgatcga acgacaactg atttccgcga aggaattagg
      301 aatccctgaa gaatatatat ttgtagaaaa agccagcggc aaagatttta aacgccctga
      361 atatcaatta atgaaacgga tgtttcgaaa aggagattta ctctatctac aatcattaga
      421 tagactaggc agaaataaac aaatgatttt agaagaatgg cgagaattaa ctaaggaaaa
      481 gcagattgat atttctgtgt tagacatgcc tctgttagat actgctagtt acaagcaact
      541 agatgggtta gagacgctta tctctgactt aattctgcaa ttactcagct atatggccga
      601 agacgagcgg aaacgaatta atagccggca aagagaaggc gtagaagctg cgaaacaaaa
      661 gggtgtaaaa ttcggcagaa agaaaattgc tttacctcct gaatttcctg cgatctattc
      721 tgactggaaa gcaggaaaga taactgcagt ctatgctatg gatcaattgg gcatgaaacg
      781 aaataccttt tatcgacggg tgaaagaata tgaacatacc ttgcaccact tttcttgata
      841 gatataacag ctattagacg ggaagggaga gtttgaaaat ggacttaacg aatcaaaggg
      901 tcgtttttac tggaacgctg cagcaaatga cacgaaccca agccatcggt ttggttcact
      961 ctttaaacgg ccattgtcaa tcaagtgtta cttcaaaaac aaatttcctg gtttgtggtc
     1021 agtattctaa gtcgttgttt gatgattctc tctatacaaa aaaggaacaa accgcgcggg
     1081 acttgattgc gttaggtcat gagattacca ttttatcaga agtcgaattt tatgcgttaa
     1141 ttagtcaaca gttgaaagaa tggcaacggt atttataatc gttagtaaaa tttacaagga
     1201 ggaaaatttt tatgaagcga aaatcttatg atcgttgggg cattgaaata actagtttta
     1261 atcgaaatat tgcttttcac gtggagtgtg actgtgggga attagcaaaa agtgtttatc
     1321 aaaaaaatta cttttactgt cctaaatgcc atcgccgata cggggtacat ggaacataga
     1381 aacgaaatag tgcggtagaa aagcgaatga atgtagtata tttctacaaa tcattcgctt
     1441 tctttatttt ataaatagtt ggtcgagaga tcccgttttc ttttgcaatc tctgcaactg
     1501 gaatgccttg ctttaacatg tccaccacgg ttttaaaaac taagcgtttt tgcggatctg
     1561 ttgagttaat agaatactcg attggtcgtc ccttatatcg gccggctttt tgagccaata
     1621 atactccttg ccgctgacgt tcacgaattt tctcacgctc gttttgtgca atataactta
     1681 gcagtgataa aaacatatcg aacatagcgg tatccaggag ttcatttcca gtattaaaat
     1741 ctaaaaactg tgcatctaat atttttaacg ctactttttt ctgtttcata aaagcaactg
     1801 tattttttat atcttcataa tctcttccta aacgatctag tgatgtaacg atgacgcaat
     1861 caccttcacg gatataattt agtaattttt gaaactcagg acgctcagta tctttaccac
     1921 tggctttttc ttggaataat ttatcaatgt taagtccttc catcattgcc aattgtcgat
     1981 ccaaattttg atcttgtgaa gatactcgaa tatagccaat ttttcccata aaaaactcct
     2041 ttttaaaagt gatataattt acatgtaaaa tttacttgta gttttactgc tatcatagca
     2101 tttttaaaaa taaattttat gtagacttaa aatttacttc atggagggtt taaatggatg
     2161 aaatcaaaag taacaaaaaa gcatttggca tttatttagg agaacctagt acagaacaat
     2221 taaaccaatt ttttggttct gttgcgaagt ttgaaaatca aacgcgccct ctctgaactc
     2281 caaatatctt aggctgttat tcccattaat accttgattt cagtagacac cgaaaagccg
     2341 aagagcgttc catttcttcg gttcttttta tatattcctc gaatggtctc catgccctta
     2401 atcgtggaag aggctgtacg gagactttga t
//
LOCUS       DAJSBL010000145         2423 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.150, whole genome shotgun sequence.
ACCESSION   DAJSBL010000145 DAJSBL010000000
VERSION     DAJSBL010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2423
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.150"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            119..2272
                     /locus_tag="ONT85_002920"
     CDS             119..2272
                     /locus_tag="ONT85_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HCR2379818.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
BASE COUNT          826 a          425 c          537 g          635 t
ORIGIN      
        1 aagttgattt tatcttgaaa ttaggtaaag aatttcttgc tccgcccatt ttttcttttt
       61 tattaagaag tttttgggta caatgaagca tgaatgtaaa taggcggaag gataggaaat
      121 gatgaaagat tttatgaaaa aaatattaaa tacttcttgg ttagaaaaaa taagaaaagg
      181 tgcacaagaa cctaagaaca aaacgtttcg ccggtcccat gttccttttc gcttaaattt
      241 tctttttttc attatattta cgttatttgt tgcgttgatt gctagattgg gttatttgca
      301 gatcgtgaac ggcgaagaga tggaggcgcg agtgaagtct acttctacga ttacggtcca
      361 agaaagtact cctcgcggaa tgatctacga ctccacagga aaagctttag ttacgaataa
      421 agcgaatcag gccatcacat atacgagagg aacacaagtt acggctaaag atttgcttga
      481 aatcgcaacg aagctgaact cgctgatcga tgttccggta gatgaaaatc tgacagatag
      541 agataaaaaa gatttttggc tggctgatcc caaacatttg aaagaagcca caaaaagact
      601 ttcggcaaaa gaaaagcaat tagggaacag cgaacaatat agtgcgatgg tcaataaagt
      661 cacagctgat gagattaatt taaacgagga gcaattaaaa attgctacca tctttaaacg
      721 gatgaatagt gcatatgaat taaacactgt ctatatcaaa aactctggtg ttactgatca
      781 agagttagct gttgtggcag aacgcgcttc tgaacttcca ggagtttcga ctggcacaga
      841 ctggacacga gaatataatg cagcaagttc actgaaaagt attcttggca gtgtgacgac
      901 tgaaaagcaa ggacttccag cagatgaagc agagaaatac ctagcaaaag gttattctcg
      961 gaatgaccgt gtaggacaaa gttatttgga aaaacaatat gaagatgtcc tgcaaggaac
     1021 caaaacccaa tatgaagttt cccttgataa tgaaggaaat gtttcgaatc aaaaagaaat
     1081 cttctctggg gaaaaaggtt ctaatttaat gttatctatg aatgcagaat tccaatcaaa
     1141 agtagaagaa attttgaaaa ggaattatca aacattgatc aacaacggca aagcacagta
     1201 ttcgcctggt gcttatgcag tggctatgaa tcctcagaca ggagaagtgc tagcaatgac
     1261 tggtttcagt catgaacaag gttccaaaga aatcaccgag aatgctttag gcacgattac
     1321 tagcgccttt gatccagggt ctgtagtcaa ggctggtact ttgacagcag gctgggcaag
     1381 taacgcgatc agcggaaatc aagttttgat tgatgagccg attcgtctgc aaggtgcttc
     1441 agaaaaaagc tctgtcttta atcgaagcgg acaagttgct cttgatgcag tcaaagcatc
     1501 ggaattgtct tctaatacgt atatgatcaa agtggcgtta aagatgttag gactagacta
     1561 tacacctgga atgggactac ctagtttaga tgaagaagct aaagcttatc agcaattgag
     1621 agactctttc aaagagtttg gtcttggtac aacgactggg atcgatttgc cgaatgaatc
     1681 tcctgggatc tcacgttctg ttgattatat gaagaagttc aatgcggata atggcaaaga
     1741 atggtatgct ccaggtaact ttaccgattt ggcatttgga cagtttgaca catatacacc
     1801 aatccaatta gctcaatatg cttcaacagt cgctaatggc gggaaacgag tacagccgca
     1861 cctagtcaaa gcgatctatg gcaatgacga aaatggaaac ttgggtgaag tgaagaaaga
     1921 aattggcaca acagtagaaa atacggtcaa tatttctgca gaaaatatgg cgatcctccg
     1981 tgaaggattc caccaagttg tccatggtac cgatccttac acaacagcca aaccattagc
     2041 cagtgcgaaa atggaccttt ccgcaaaaac agggactgca gaaacggttg cggaaggtca
     2101 tccagatatt gcgacagtca atagtaatat cgtggcatac ggaccaacag acaatccgga
     2161 aattgccatc agtgttgtct tgccgaactt attagacgaa caagaccaca taaacttgac
     2221 gattgctaaa gaaataatgg acgcgtatta tgatatgttc atggcaaaat aaaaaagagg
     2281 tcgtgaaaaa gccgcttagt ttcgagtatg aagaaaaatt ttgtgaaaat agcttctcac
     2341 atttttgctt aaatttgact tcataccgag aaactggctt ttgaacaccg tttactcggc
     2401 aaaaaaggtt gtgacaaaaa ttg
//
LOCUS       DAJSBL010000146         2407 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.151, whole genome shotgun sequence.
ACCESSION   DAJSBL010000146 DAJSBL010000000
VERSION     DAJSBL010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2407
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.151"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            5..1150
                     /locus_tag="ONT85_002921"
     CDS             5..1150
                     /locus_tag="ONT85_002921"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HCR2379819.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            1321..1779
                     /locus_tag="ONT85_002922"
     CDS             1321..1779
                     /locus_tag="ONT85_002922"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HCR2379820.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            1938..>2407
                     /locus_tag="ONT85_002923"
     CDS             1938..>2407
                     /locus_tag="ONT85_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HCR2379821.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
BASE COUNT          802 a          452 c          455 g          698 t
ORIGIN      
        1 aaacatgcac attcaaacaa aattgatcca cggaggcatc agtgaagatc ccacaacagg
       61 agctgttagt gtacctattt atcaaacatc cacttatcgg caagatggcg tcggccagcc
      121 taaacaatat gaatattcaa gatctggtaa tcctactcgt tttgcattgg aagaactgat
      181 tgctgatttg gaaggcggtg ttcgaggatt cgctttttct tctggtcttt ctgggattca
      241 tgccgttttt tctctttttc aagctggcga tcatatctta ttaggagatg atgtctatgg
      301 aggaacattc cgtctatttg acaaagttct tactaaaaat ggtctcgagt acacgatcat
      361 agatacgagt aatcttgata aaatagaaca atccatcaaa cccaatacaa aagcacttta
      421 tcttgaaaca cctagcaatc cgcttctaaa gattactgat ttagaaaaaa gtgccactct
      481 ggctcatcaa catggattga tagttattgc tgacaataca tttgctactc cttattttca
      541 aagaccttta gatctcggtt ctgatatcgt tgtccatagc ggtaccaaat atctaggcgg
      601 acacagcgat gtagtagctg gtttagtgac ttctaaccat aaagatctag ccgatcagat
      661 cggattttac caaaatgcta tcggagccgt acttggtccc caagatagct ggttacttca
      721 gcgcggaatc aagacactaa gtgttcgtat ggaggaacat caaaaaaatg cttttgtggt
      781 agctgatttt ctattttctc atccagcagt agaaaaggtc tactatccag gattacccga
      841 tcatgaactc catggagttg ctaagcagca gatgagcggc tttagcggaa tgatttcttt
      901 tactttaaaa aatgaagaat ctgctattcc atttgttgaa tcactccaac tatttacttt
      961 agcagaaagt ttaggtggcg tagaaagttt agtggaaatt ccttctgtca tgactcatgc
     1021 gtctattcca aaagaaaaac gagaagaggc tggcataaag gacggcttga tccgtctttc
     1081 tgtaggaatt gagtatggtc aggatctcat aaacgattta gctcaggcat ttgacagaat
     1141 caagaactag aagtcccaat aaacgactta gcagaaaaat ctaaaaaata tttcagattg
     1201 ctacaccttg gaaacgataa gtgttagaat caagtagaaa gacgtttttg taaatttcac
     1261 taaataattc tttagtgaaa agcatcaaaa cgaatgaaca aacagatagg agtcttacaa
     1321 atgagaatga atgtcgaaag ttttaatctt gaccatacaa aagtcaaagc accttatgta
     1381 cgtgtagctg atcgaaaaac gggtgcgcat ggcgatgtca tcattaaata cgacgtccgc
     1441 tttaaacaac caaataaaga acacatggac atgccaagtc tacattcact tgagcacttg
     1501 actgccgaac taatccgcaa tcacgcagat tacatcgtag actggtctcc aatgggatgc
     1561 caaacaggct tttacttgac tgtattgaat catgaaagct acgaagacat tttagatgtt
     1621 cttgaagcaa caatgaaaga tgttcttgaa gctactgaag tacctgcaag caatgaaaca
     1681 caatgtggct gggctgccag ccatacacta gaaggtgcac aagctttggc tcgtgaattt
     1741 ttagcaaaac gcgatgaatg gtctgaagta gaagcatagt ttctaaaaga acgtaaagat
     1801 gagaagcaat atagctatct tatcttcgta ttttattaaa tatttattgt ctgaaaagag
     1861 ttctaaacgg gaaaacaaat agctagtttt cagtttagcg ctctttttat tatataaaat
     1921 agaaaggaga aaactttatg tctatttata attttgaaac tactttagaa gacggcactg
     1981 tctattcatt gaaaaaatat gcaggcaaac cgataatcat tgttaacact gcaacaaaat
     2041 gcggtctagc acctcaattt aaacaattag aagaacttta ccaagcttat cagacacaag
     2101 gtctggttat cttaggcttt ccttccaatc aatttaaaca agagctagat tcagcgactg
     2161 aagcagcaga agcttgtaaa accacttatg gcgttacctt ccctatgcat caattgattg
     2221 acgtaaatgg agaaaataca gatcctcttt ttacttatct taaaaatgaa gctcctggta
     2281 ctgtcactaa agcaataaaa tggaatttta ctaaattttt gattgataga aatggcaaag
     2341 taatcgaacg atttgcacca caaacttctc cattgaaaat gaccgctgca attgaatccg
     2401 ttcttta
//
LOCUS       DAJSBL010000147         2400 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.152, whole genome shotgun sequence.
ACCESSION   DAJSBL010000147 DAJSBL010000000
VERSION     DAJSBL010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2400
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.152"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            207..2069
                     /locus_tag="ONT85_002924"
     CDS             207..2069
                     /locus_tag="ONT85_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCR2379822.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2069..>2400
                     /locus_tag="ONT85_002925"
     CDS             2069..>2400
                     /locus_tag="ONT85_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCR2379823.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          866 a          353 c          466 g          715 t
ORIGIN      
        1 aaaaatttga aaaagcgttt gattatttat attacgactt agtgcaataa aaaacgcttt
       61 ttaacagtac atgtagtcgt ctttataata gataaactct gtaatattag ttaaaaagct
      121 ataagttgca cttagttaag tgcaaacatt tcatattgtc gttagtttag aaaacgctta
      181 tattagcaat gtaaggagag atgataatgc agcagctaac aaaaagagaa aggcagcttt
      241 tgatttactt ggtggaaatg tctgaaccgg taacaatcaa aaagttggct gaaaaagaaa
      301 atgttagtgt tcggacgata aaatacgatc tagatgatat aagagagtgg ctgaatgagc
      361 ggaagcaagt cctatatagc aaacgtagtc agggaatttg gatgaatatt agtagctctg
      421 atcgaatcaa attaaaaagc gaattgatgg atgttaatcg ttttgaaata tttgcagatc
      481 aaagtctccg aatcaaacgt ttactaatta tgcttttagt aacaaaagaa gctattactg
      541 gaatgaaaat ggccaacgca ttagatgtca gcaaagatac gatcatgaat gatttagatg
      601 tgctggaaaa taattttctt aaggaaggat tgaccttgaa tcgtcaggcg cgaaagggtt
      661 tttggatcat aggagaagaa cgtctagttc gattaacgat tgaagagatt cttcaaaaag
      721 agttcacgga ttatgacatt tataaattaa tgtcattgtt attgaatggc ggtgagactg
      781 aatactttga aatgtattct gctactgcta cacctatcca agaggtattt aatcaagtaa
      841 tcattcgaat gcgacactta ctggaatttg aaaatctaga gaaactgaat tacgcggaat
      901 tactaaatat tctgattcga gtgacaattg ctaccgttcg cttacgcaaa gaggctacga
      961 ttggtcgtta tcaattggtt gctgaacaag aattagacaa aaacgatctt tcttatcgcg
     1021 tcatgcagca ggtctttgat tattatcagt tgccactgtt cgaggatgaa tatcattata
     1081 tctatagtga tacattcgac acgattcacc agcaagacgt catgaaacta gttgagaatg
     1141 tgatttataa agtaagtaaa gcaatcaacc attcttttta tcaagatcca caattgttga
     1201 cgaatctgta tgctcattta tccatgcgat tatcgagaaa gaaaaaattt gtcaatgaat
     1261 ataatccttt taaagaagat atcaagcaga aatacccgga attatttgat gcgatcaagc
     1321 aagcagttcg acaagaaata agtgaacaaa atattctgat caatgattct ttcgtggcat
     1381 atattgcctt acactttttg gtttcctacg aaagagaaga agatctacga agcgttagag
     1441 ccgtctacat ttgttcaaca gggttgggtg tgactagtct gatcaagcaa aagatcaatg
     1501 aagaattaaa taatatcact atcgtggcat ttgcttcagt attgaatgcg caagaaatga
     1561 ttgctaccaa agatcctgat ttggtgatta gtatttttcc aattaatggc atcgatcgtc
     1621 ctttcatcaa agtccatcca ttacccactg aacaggactt gaaaaaaatt caacaaataa
     1681 taaaaaaaat acttgcttct tccaatcagc aggaaaggca taacttgaat cttagagaaa
     1741 cgtccatcaa taaagatact ttagaagact ttagcaagga attgattttg aaagcttata
     1801 cgatttatga acgtttactt gtcattttgg aagcagaatt gatccaagaa tatcgagaag
     1861 cttttttgct tcatgtattc ttaatggttc atcgtatcac atttgatcaa caatatatgg
     1921 cagaaggtcg tcaagtagac cagattattt tagacaaagg aacactggta ggaaaaatcg
     1981 aagcagtttt tgcagaaaat aatttaggaa tcaatcagtc agaaatcagt gcattattct
     2041 catatataaa agaaggagcg tcaagttagt gaaaatgtca gacgaggctt taaaaattat
     2101 cgaggaaagt caaaatcggg aaaagttaaa acaagtgatt gatctaattg aagagcgatt
     2161 agtagaacat gctattgtac caaccgagtt gcagtggacc attctaatta atcacttgaa
     2221 tgagatgctg aaacgttctc aaagaaagga aacgattccc gtagtcgatc gggatttgtt
     2281 caaagaagta tccaaagaag ccataactat ttctgatgaa atagttagaa gtatcggtaa
     2341 tttaaccgat gatgaaatat atgttttgtc gattcatttt gaaacagcaa aaaataatta
//
LOCUS       DAJSBL010000148         2232 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.153, whole genome shotgun sequence.
ACCESSION   DAJSBL010000148 DAJSBL010000000
VERSION     DAJSBL010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2232
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.153"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..544
                     /locus_tag="ONT85_002926"
     CDS             <1..544
                     /locus_tag="ONT85_002926"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCR2379824.1"
                     /translation="ISKENLLNQDFSTETICEKWAADITYIPTKKNGWCYLSSIMDLH
                     TKRIISYTFSKRMTVDCVIQTLNKAKIHYHIPEGMILHTDLGSQYTAREVEQWLKTNK
                     IRHSYSRKGTPYDNAGIESFHASLKKEEVYTTSYSDFEEANRALFSYIEGFYNRNRIH
                     SSIHYLTPQEFEELAKEKMA"
     gene            complement(900..1472)
                     /locus_tag="ONT85_002927"
     CDS             complement(900..1472)
                     /locus_tag="ONT85_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR2379825.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENCVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(1488..2093)
                     /locus_tag="ONT85_002928"
     CDS             complement(1488..2093)
                     /locus_tag="ONT85_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell filamentation protein"
                     /protein_id="HCR2379826.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
BASE COUNT          700 a          454 c          338 g          740 t
ORIGIN      
        1 catttcaaaa gagaatctct taaatcagga cttttctact gaaacgatct gtgagaaatg
       61 ggcagctgac attacgtaca ttcctactaa gaaaaatggt tggtgttact tatcttcaat
      121 catggattta cacacgaaaa gaattattag ttatacattt tcaaaacgaa tgactgtgga
      181 ttgtgtcatt caaacattga ataaagcaaa aatacattat cacattccag aaggaatgat
      241 tctacacact gacttgggca gccaatacac agcaagagaa gtggaacaat ggcttaaaac
      301 caacaaaata aggcattctt atagtagaaa aggaacacct tatgataatg ctggaatcga
      361 atctttccat gcctcattga aaaaagaaga agtctacacg actagctact cagattttga
      421 agaagcaaat cgagcgctat ttagctacat tgaaggattt tataaccgaa atcgaatcca
      481 tagctcgatt cactatctaa ccccacagga atttgaagaa ttagcaaaag aaaaaatggc
      541 ttaaccgtct ctactaaaga caatcaacac cgtagggata tggagataaa atcaatagag
      601 tcaatagatt atttatcgat aataacacta aaaaagccac gttcctaaac gtgacttacg
      661 ctgaacgata tgtgttgttt ggtagacgaa caaatcgttc attataaata tatgatggta
      721 tcctaatata gtaagcgtgg ctagcatacc tttaattact ttacctttta cagttaccat
      781 acacattttt tgagcagaaa tcaagtaaat aaaaaaatat tatatttcac aacatgctaa
      841 tagactcata tctagaacgc attaagtatt tacctgtctg gttcgctgag acttcttttt
      901 taactttctt caattttctt ctttattcta aaaagcgtac tgcgactgaa accggttata
      961 gcttcaactt ccttatacga tttacctgaa gtaagcagtt catatgcgtg cctcttcttc
     1021 ggtgtgattg ttgctttcgg acgtccttct ctgaaattag gattattctt tcttgcaaac
     1081 atttttcctt cttgggtacg ttccacaatc atatcacgtt ccatttctgc tacactcaac
     1141 aacgtccgtg tcaccattcg ccccatggac gtattttcaa tcgttcctag attcaaaacc
     1201 ttgatcgtta cgttatcatc gagtaaaggt tcaatgatct ctaatgcttc tctcgtgtta
     1261 cgagcaaatc gatcaagctt cgtaacaact atttcatctc caggcttcag accagccaac
     1321 agctgattga attttggccg atcccgtttt gtacccgtaa atttttctga aaaaatattc
     1381 ttcttctcta cgcagttctc aagcaactgt tgaatttgtt cttttgtact ttgagaagcg
     1441 gttgagacac gtgcatatcc atatttcgtc atttggtcca cctactttta caaatcatct
     1501 atcgtatact cagaataccc ttcatagaaa tagctgacat cgattccttt catatataac
     1561 tcgcgatcgt caattttatc agtcagagca ttggaaatta gatagcgtat ttccaaatcg
     1621 tttatgggac ttcgctccat tgcagaaaga tagtctgctt tatcgattaa gttccaatct
     1681 acaacttttt gcaattcttc ctttaaaatg agatccagcc aaattctagt acttcggcca
     1741 tttccttctc gaaatgggtg agcgatattc atctccacat atttcttgat aatctcttca
     1801 aaagaagttt gtggcatctg atcaatatgc tgtaacgaat gttctaaata cataactggt
     1861 gcaaatcgaa aatttccttt tgcaatattg accgatcgga tttttccggc aaaatcgtaa
     1921 atatccgaaa atagaaattc atgaatttca gataaacctt taaatgtacc aacttcgatt
     1981 tcatttattt ttccacaatc gtacaattct ttcgcttttt gtttactgag tttttcttca
     2041 accttagcca actcaacttg attggttaat cccaatttat tctctaaaat catgcgaaat
     2101 ctccttaact ttatgacata agtttatgaa acactctcaa ccccattata tcgcgatttt
     2161 gtttatgtgt caaaaactat taagtttctg acccccgata gatttttcaa gtgtctctat
     2221 tttgcacagt ct
//
LOCUS       DAJSBL010000149         2173 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.154, whole genome shotgun sequence.
ACCESSION   DAJSBL010000149 DAJSBL010000000
VERSION     DAJSBL010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2173
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.154"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..378
                     /locus_tag="ONT85_002929"
     CDS             <1..378
                     /locus_tag="ONT85_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HCR2379827.1"
                     /translation="KNVSTEDFFNYCRLEESGVTVTSGTDLPLFITDIPESIIRAVFR
                     RFPEGEEVWEKETSFTTTELLKSFTSNSFKVNGLSEYGEIKVGKSASLAVFSEDLCTD
                     DWQKVMAAEVIATYLDGDLVYKK"
     gene            426..1826
                     /gene="yfcC"
                     /locus_tag="ONT85_002930"
     CDS             426..1826
                     /gene="yfcC"
                     /locus_tag="ONT85_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HCR2379828.1"
                     /translation="METMKRKFTFPHSYTLIFILIILAAILTWIIPSGQFETVVENVA
                     GMEKTTVVPGTYHQIDKADYGQGFSSILMAPAEGIISAVEVVAFVLIVGGAFGIILKT
                     GAIDRGLFTLAKVLGDKGILVIPMSMILFSLGGSTFGMSEEVIPLFAVFISLMFSLGY
                     DSMTAILILFLSTQAGYVGSTINPFNVLIAQGVAGVYGNPLLWFRMLSWIVITGISIV
                     FTMLYASRVKKNPLSSIVYESDLKVKHKFQNFDTEKEFSTQDKLIIAGFIVGLGIMIW
                     GIISKGWYMVEISALFLGLGLFAGGIARFSQKEIAEAFVEGCSDFAYAAVIIGLARGV
                     LVILEGGMIIDTILNSLANLLGGLPKFLFTTILLISQVIVSFFVPSSSGAAALTMPVM
                     APLGDLMNVSRDVVVLSNQFGNGLMNIISPTSGVLLAGLAIAEINFSKWFKVGFKIFA
                     ILTLVSAVLLYIATLF"
     gene            1859..2026
                     /locus_tag="ONT85_002931"
     CDS             1859..2026
                     /locus_tag="ONT85_002931"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2379829.1"
                     /translation="MIKINQLAGMNIHYLFYSLKEFFAYQQKIGISSVELWGGAPHFF
                     IDAFTGSVAKF"
     gene            2080..>2173
                     /locus_tag="ONT85_002932"
     CDS             2080..>2173
                     /locus_tag="ONT85_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017370594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HCR2379830.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREV"
BASE COUNT          613 a          362 c          469 g          729 t
ORIGIN      
        1 aaaaatgttt ccacagagga cttctttaat tattgtcgat tagaggaaag tggtgtaaca
       61 gtcactagcg gtacagattt accgctgttt attactgata ttcctgaatc gattataaga
      121 gcagtattcc gtcgttttcc tgaaggagaa gaagtctggg agaaagaaac aagcttcacg
      181 acaaccgagc tgctgaaaag ttttacaagc aacagcttca aagtaaatgg tttatcggag
      241 tacggagaaa taaaagtagg gaaaagcgcc agcttagctg ttttcagcga ggacctctgt
      301 actgatgact ggcaaaaagt gatggctgcg gaagttatcg ctacctatct tgatggtgac
      361 ttagtttaca agaaataatg cttttcgata taacgttata aaacgaatta aagagaggtt
      421 aaaaaatgga aacaatgaaa agaaaattca ccttcccaca tagttataca ttgattttta
      481 tcttaattat tttggctgca attttaactt ggataattcc tagtggtcag tttgagacag
      541 ttgttgaaaa cgtcgcaggt atggaaaaaa caacggttgt tccgggaacc tatcaccaaa
      601 tcgataaggc agattatgga caaggttttt cctctatact aatggcacca gctgaaggga
      661 ttatttcagc tgttgaggtt gttgcttttg ttctcatcgt tggtggtgcc ttcgggatta
      721 tcctaaaaac gggagccatc gatcgagggc tgtttacctt agcaaaagtt ctgggagaca
      781 aggggatctt agttattccg atgtcgatga ttctattcag cttaggagga tcaacctttg
      841 gcatgagcga ggaagtcatt cccttatttg cggtgtttat ttctctgatg ttttctcttg
      901 gctatgattc catgactgcg atcttgattc tatttttaag tacacaagcg ggatatgttg
      961 ggtcgacgat taatcccttc aacgttttaa tcgcacaggg agtagctggc gtttacggaa
     1021 atcccctgct ttggtttcgg atgctatcct ggattgtcat tactgggatt tcaattgttt
     1081 tcaccatgct ttatgctagc agagtcaaaa agaatccact gtcctctatt gtttatgaga
     1141 gtgatttaaa ggttaagcat aagtttcaaa attttgacac tgaaaaagag ttttcaacac
     1201 aagataaact aattatcgcc ggttttatcg taggtttagg aatcatgatt tggggtatca
     1261 tttccaaggg atggtatatg gttgaaattt ctgcactatt cttaggatta ggtctttttg
     1321 ctgggggcat cgctagattt tctcaaaagg aaattgctga agcttttgtc gaaggctgtt
     1381 cagatttcgc ctatgctgct gtaatcattg ggctagctag aggagtcttg gtaatcttgg
     1441 aaggtggaat gattattgac actattctaa acagcttggc taacctttta ggtggtcttc
     1501 caaaattcct gttcactacg attctactca tttctcaagt aattgtttcc ttttttgttc
     1561 catcctcctc aggagctgcg gcattaacga tgccggttat ggcaccattg ggtgatctaa
     1621 tgaatgtaag tcgtgatgtt gttgttctaa gtaaccagtt tggaaatggt ttaatgaata
     1681 ttatctcccc aactagtggt gtattactgg caggattagc catcgcagag attaatttct
     1741 ctaaatggtt taaggttggc ttcaaaatat ttgccatctt aacccttgtt tctgctgttt
     1801 tgttatacat tgctacactc ttttagtctt aatcatccat taataggagg caatagttat
     1861 gattaaaata aatcaattag caggaatgaa tatccactat ttattttatt ctctcaaaga
     1921 atttttcgct tatcaacaga aaattggtat ttcttctgtt gaactttggg gaggtgctcc
     1981 gcattttttc atagatgctt ttactggttc tgttgcaaag ttttaaatct actatcaaat
     2041 aaggtagaat aatagaaaaa gatagcagga ggaatgacga tgaatcattt taaaggaaag
     2101 caatttcagc aggatgtgat tattgtagcc gtgggctact atcttcgtta taaccttagc
     2161 tatcgtgaag ttc
//
LOCUS       DAJSBL010000150         2149 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.157, whole genome shotgun sequence.
ACCESSION   DAJSBL010000150 DAJSBL010000000
VERSION     DAJSBL010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2149)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2149
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.157"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..914)
                     /locus_tag="ONT85_002933"
     CDS             complement(<1..914)
                     /locus_tag="ONT85_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_042964654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HCR2379831.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIY"
     gene            1342..1905
                     /gene="efp"
                     /locus_tag="ONT85_002934"
     CDS             1342..1905
                     /gene="efp"
                     /locus_tag="ONT85_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HCR2379832.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
BASE COUNT          727 a          380 c          414 g          628 t
ORIGIN      
        1 aagtaaatgt tttttgataa atagcggtct gctgcgtctg gaaaaatcgt aacgacacga
       61 tgaccaggtg gtaaacgacg aatttctctt aatgctgctg caaaagccgc accactcgaa
      121 cttccaacta atagtccttg ttctcgtgct aactgcctag tatagttaaa cccttctaca
      181 tcagagattg tctcgatctg attgattgaa aggggagaaa gaaacggcgg aatgaattct
      241 acaccgatac cctcaatttc gtgaggggct ggatcaccac catttaacac agagccctct
      301 ggttctacac cgattattcg aatgtcggga tatttttctt tcaaaaaggt agaagttcca
      361 gcaaaggttc caccactccc aattcctgct acaaaactat ggatattttc tttgatttca
      421 tgaaagattt ctggccctaa cgtatgatag taagcagcag gattatcttt attttcaaat
      481 tgaagaggca gatagctgtt tgatatctcc tctgcaagtt ttttactttt gctgatggca
      541 ccgatgattc cttgctcact aggagaatgg atgatctttg ctcccaatgc tttcatcaat
      601 tgttgttttt ctaagctaaa cttctctgga acaacaaaaa ccgttcttaa tttgtatgtg
      661 agtgcggcca gagcgatccc aattcctgta tttccggcag ttggttcgat gatcgtcgtt
      721 ttttcattga tttttccttg ggcaaatcct tctttgatca aataactgcc caatctatct
      781 ttgatacttc ctccaggatt taaatattct agttttgcat aaatagcaga acctttaggc
      841 atagaatagt gctgattgtc aaagcgataa agcggtgtgg aaccaatagc ttctgtaatt
      901 gtcgagataa tcatgtgggc ctcctaaaaa aagataataa aaaaaggagc tgactattaa
      961 atcagctcct tgaaagacat agagaaaaat tgagtagata aaaagataag ttacctggta
     1021 ctcattctat gaagggcaat taatagacat gacaaatagt atgctgcttt tatgcaccat
     1081 acaacagcaa cgattcaatt gtttttggtt gctgactgtc atgtgtatcc ctccaagtaa
     1141 aatttttatt aacgatagca tgaaaaaaaa taaaagtcaa aggcttgaaa gaaaaaaata
     1201 tttcgatagc atcgtttacc cgtgtttatt tttctgacaa ataggcatga gaccttaaaa
     1261 gatagagaat actctagttt tctggccggt tgtctgatac aataaagttg attatgataa
     1321 agaaaagaag gagcaaaaat aatgatctca gcaagtgatt taagagccgg tatgactttc
     1381 gtacaagacg gcaaactgat taaagttctg gatgcaagcc accataaacc tggtaaagga
     1441 aatacagtta tgcgtatgaa actgaaagat gtccgttcag gtgcaacata cgacacaacg
     1501 ttcagaccag aagaaaaatt tgaaaaagca catatcgaca caaaacctgt acaatatttg
     1561 tacacgatgg atgatacagc tttcttcatg gatttggaaa catatgagca atatgaaatt
     1621 cctgtagaaa cagtagaaga agaaatgaaa tatatcctag aaaacatgga agtaaaaatc
     1681 caatttttcg gtagcgaagt catcggcgtt caattaccaa caactgtcgt attacgtgtt
     1741 gtggaaacac aaccttccat caaaggtgca acagtcactg gttcaggtaa gccagcgaca
     1801 atggaaactg gcttagtagt caatgttcct gacttcatcg aaacagatga attgttggaa
     1861 atcaatacag cagaaggttc ttatgtaaaa cgtgcgtcaa aataattcgc taacggattg
     1921 ataagaattt gaaaacagag cttgacctct tgaaaggcag atcatcggat ctgcctttca
     1981 agaggttttt tgtggaatca gcaaattgaa aaataaaaaa gtatttgatt ttatttatat
     2041 tttgaaagat gaatggcaaa agctagagaa agaagaaact gcacttattt atgaagaaat
     2101 tgaaatgatt cataaaattg aaaatatccg aactattccg aactatatt
//
LOCUS       DAJSBL010000151         2135 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.158, whole genome shotgun sequence.
ACCESSION   DAJSBL010000151 DAJSBL010000000
VERSION     DAJSBL010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2135)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2135)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2135
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.158"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..2135)
                     /locus_tag="ONT85_002935"
     rRNA            complement(<1..2135)
                     /locus_tag="ONT85_002935"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          445 a          633 c          428 g          629 t
ORIGIN      
        1 tacctatcct gtacatgcgg tacagacact caatatcaaa ctacagtaaa gctccatggg
       61 gtctttccgt cctgtcgcgg gtaacctgca tcttcacagg tactaaaatt tcaccgagtc
      121 tctcgttgag acagtgccca aatcgttacg cctttcgtgc gggtcggaac ttacccgaca
      181 aggaatttcg ctaccttagg accgttatag ttacggccgc cgtttactgg ggcttcaatt
      241 cgtaccttcg cttgcgctaa gcactcctct taaccttcca gcaccgggca ggcgtcagcc
      301 cctatacttc atcttacgat tttgcagaga cctgtgtttt tgataaacag tcgcttgggc
      361 ctattcactg cggctgatcg tatgatcagc accccttctc ccgaagttac ggggtcattt
      421 tgccgagttc cttaacgaga gttctctcgc tcaccttagg attctctcct cgactacctg
      481 tgtcggtttg cggtacgggc cgctgttttc tcactagaag cttttctcgg cagtgtgaca
      541 tcaggaactt cggtactatt atttccctcc ccatcacagc ttgtccttaa gaaaagaagc
      601 atttgactcc tcttcagact tactgcttgg acagacattt ccgatcgtct gcattcctta
      661 gcctcctgcg tccctccatt gctcaaacaa aaacaacggg tacaggaata tcaacctgtt
      721 atccatcgcc tacgcctgtc ggcctcggct taggtcccga ctaaccctgg gcggacgagc
      781 cttccccagg aaaccttagt cattcggtgg acaggattct cacctgtctt tcgctactca
      841 taccggcatt ctcacttcta agcgctccag cagtcctcac gatctgcctt caacgccctt
      901 agaacgctct cctaccaata cacctaaagg tgtactccac agcttcggta atatgtttag
      961 ccccggtaca ttttcggcgc agggtcactc gactagtgag ctattacgca ctctttaaat
     1021 ggtggctgct tctaagccaa catcctagtt gtctgtgcaa ccccacatcc ttttccactt
     1081 aacatatatt ttgggacctt agctggtggt ctgggctgtt tccctttcga ctatggatct
     1141 tatcactcac agtctgactc ccggatatga atgaatggca ttcggagttt atctgaattc
     1201 ggtaacccga gatgggcccc tagtccaaac agtgctctac ctccatcatt ctcaattccg
     1261 aggctagccc taaagctatt tcggagagaa ccagctatct ccaagttcgt ttggaatttc
     1321 tccgctaccc acacctcatc cccgcacttt tcaacgtacg tgggttcggt cctccagtgc
     1381 gttttaccgc accttcaacc tggacatggg tagatcacat ggtttcgggt ctacgactac
     1441 atactcaaac gccctattca gactcgcttt cgctgcggct ccgtctcttc aacttaacct
     1501 cgcatgcaat cgtaactcgc cggttcattc tacaaaaggc acgccatcac ccattaacgg
     1561 gctttgactt gttgtaggca cacggtttca ggttctattt cactcccctt ccggggtgct
     1621 tttcaccttt ccctcacggt actggttcac tatcggtcac tagggagtat ttagccttgg
     1681 gagatggtcc tcccggattc cgacggaatt tctcgtgttc cgccgtactc aggatcctcc
     1741 taggtgtctt ccacatttcg tctacggggt ttttaccctc tttgactgac ttttccaagt
     1801 cattcgacta tctgaaagaa ctaccatatt ggagtcctac aaccccaaca agcaagcttg
     1861 ctggtttggg cttttcccgt ttcgctcgcc gctactcagg gaatcgaatt ttctttctct
     1921 tcctgcaggt acttagatgt ttcagttctc tgcgtctacc tcgaatgtgc tatgtattca
     1981 cacaatcgta acatcctatt aaagatgttg ggttccccca ttcggaaatc tctggatcat
     2041 agcttacgta cagctcccca aagcatatcg gtgttagtcc cgtccttcat cggctcctag
     2101 tgccaaggca tccaccgtgc gcccttattc actta
//
LOCUS       DAJSBL010000152         2038 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.159, whole genome shotgun sequence.
ACCESSION   DAJSBL010000152 DAJSBL010000000
VERSION     DAJSBL010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2038
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.159"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            200..742
                     /locus_tag="ONT85_002936"
     CDS             200..742
                     /locus_tag="ONT85_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2379833.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            898..1347
                     /locus_tag="ONT85_002937"
     CDS             898..1347
                     /locus_tag="ONT85_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HCR2379834.1"
                     /translation="MLSEKTDLENTEAFNKESVKDLRKLPTKDINGKDFTSKDFEKYD
                     LTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIVGVVTDPVDDNGENKEAIEKSKL
                     IHEKTKASYPFLMPDGMATLFLTKIIRCCECCGVMATIALDNEHPRI"
     gene            complement(1377..>2038)
                     /locus_tag="ONT85_002938"
     CDS             complement(1377..>2038)
                     /locus_tag="ONT85_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3-like element ISEfa8 family transposase"
                     /protein_id="HCR2379835.1"
                     /translation="RIGRIMKRRGLTSTYTIAHFKGQRTACNEAKTANVLDRTFTQEQ
                     PLEAIVTDLTYVRVGKKWHYICLILDLFNREIIGYSCGEKKDASLVKEAFGRIPYSLT
                     DVKLFHTDRGKEFDNQTIHEILNGFGITRSLSRKGCPYDNAVVESTYKSVKVEFVHQY
                     QFETLAQLRLELFDYVHWWNYLRLHGTLAYETPIQIRQQRLAKRILDNERGSDTSGEA
                     A"
BASE COUNT          733 a          321 c          390 g          594 t
ORIGIN      
        1 attgatgaag cagatcccaa agggctgctt catcaatttt ttcgtaagtg tccgtacact
       61 tactgtgctt gctcatatat taatcgatca tatactaatc gtgtaagaac aactttaagc
      121 ataatactta tattgttata ccatagtttg gaaaaataaa aaattatatt tcatcaacgt
      181 ttcataaaag gtgtgtagaa tgttattgaa agtagctata aaatgctatt gtgtaaaaaa
      241 taaaggagga ataatttatg aaaaaatagt accaagagga gcatgcctat tacttgcatt
      301 atgtttgagc ttaagcgcag ttgggtgtca aaaaagtaat gaaaacaata aaataaaaga
      361 aggacaatcc ataagttctg aagaggaagg catgtcaatt aatgcaaatg gcaaaaatga
      421 aacttttaag ccatcagatt atacattgga ggcaaagaaa gaatatgttt atgaatattt
      481 agggcttaaa tttaaactct cagacaaatt tagaaattat atagctgata aaaagatagc
      541 gatgttagat gatcaaagtc ctatagataa agaactaaag tatgctattt tgacatttga
      601 gaaaatgaca gaagaacaaa agaatgcagt tatagaaaag atgggcgatg gatataaaaa
      661 ttggcaaaat gagcttgaaa gaattgggac tattggtata tttgaaaaaa atacatcaga
      721 agaaaaaaat ctaaaattat aaaatgtgat actcatacta aaataggagt ttcaagtgat
      781 ggaaaatacg attgctattt aagcacaaat agcggagctg aaagtaatct tctggatgaa
      841 ttaaaaagaa ctgagattca aattatagat aaaaaagaac gtcctaaaaa tggttttgtg
      901 ctatcagaaa aaactgattt agaaaataca gaagcattta acaaagaatc ggttaaagat
      961 ttacgtaaac taccaacaaa ggacataaat ggcaaggatt ttacaagtaa agattttgag
     1021 aagtatgatc ttactatggt aaatgtattt gcaacttggt gtacagcttg tgtaaaggaa
     1081 attcctgatt tggttgaggt tcagaatgaa atgaaaagca agggtgtaaa tatcgttgga
     1141 gttgttacag atcctgttga tgataacggt gaaaacaagg aagcaattga aaagtcaaag
     1201 ttaatacatg aaaagacaaa ggcttcatat cctttcttaa tgccagatgg aatggcaaca
     1261 ctttttttga caaaaattat aaggtgttgt gaatgctgcg gtgttatggc tacgattgcc
     1321 cttgacaatg agcatcctcg gatctgattt tctccggcag aagcacaatc attcaattac
     1381 gctgcctctc cagaggtatc agatccgcgc tcattatcaa ggattcgctt cgccaatctc
     1441 tgttgtcgaa tttggatcgg tgtttcatac gccaacgtgc catgtaagcg tagatagttc
     1501 caccaatgca cataatcaaa caattctaga cgtagctgtg ccagtgtctc aaattggtat
     1561 tgatgcacga attctacttt gacagattta taggttgatt ccacaacggc attatcatac
     1621 ggacaaccct tcctactcaa tgaacgagta attccaaaac cattcagaat ctcatgaatg
     1681 gtttggttat caaattcctt tccccggtct gtatgaaaaa gcttgacgtc tgttaaagaa
     1741 tacggtatcc gtccaaaggc ttcttttacc aatgaggcat ctttcttctc accacaggaa
     1801 taaccaataa tttctcgatt aaacaaatca agtattaagc agatataatg ccactttttc
     1861 cccacgcgaa cataagtaag atccgtaacg atggcttcca atggctgttc ttgtgtaaag
     1921 gtccgatcta atacattcgc tgttttcgct tcattacaag ctgttcgttg ccctttaaaa
     1981 tgagcgatcg tataggtaga tgtcaatccg cggcgtttca tgattcgacc gattcggc
//
LOCUS       DAJSBL010000153         1802 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.160, whole genome shotgun sequence.
ACCESSION   DAJSBL010000153 DAJSBL010000000
VERSION     DAJSBL010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1802
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.160"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            100..666
                     /locus_tag="ONT85_002939"
     CDS             100..666
                     /locus_tag="ONT85_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCR2379836.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            811..898
                     /locus_tag="ONT85_002940"
     tRNA            811..898
                     /locus_tag="ONT85_002940"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:843..845,aa:Ser,seq:cga)
     gene            1232..>1802
                     /locus_tag="ONT85_002941"
     CDS             1232..>1802
                     /locus_tag="ONT85_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HCR2379837.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNG"
BASE COUNT          622 a          275 c          351 g          554 t
ORIGIN      
        1 tttaaaatat aacgttttga gtaaaatgtt tgcaaaaaat gctaaaaata gggtacaata
       61 aaacaacata tactgcagta aagaagtggg ggagttttaa tgagaagaaa agtctatacc
      121 aaggatcaaa ttctaaaagc tgcttatgat gttgttgcaa aggaagggtt taaaggattt
      181 actgcgagaa atattgctaa aaagatgggg atttcgacac agccgattta tctggaattc
      241 aaaaacatgg aagatttaaa aaatacgttg ctggattcta tctttgaaca actaaaagaa
      301 gatgttttcc ctattgaacg tactggtgat aaaatcattg atctcggttt gaactatatc
      361 tactttgcaa aaaagcatca tagtctttac atggcattgt acattgatga acatggggac
      421 ggaaagaaaa tgcatgattt ttcatacaat tatttccgtg aaatagttaa agaagatgaa
      481 agatacaatc aattatcgga tgaatatgtc gatgctctgc atactggtac atgggtagca
      541 gtgactggct tagcgtcatt gatgtcatca aacatcatgc atccaacaga agaacaaatc
      601 attgcactga tcaaacggac aattgactcg attttagcat tagataatcc tgaaataatc
      661 agatagtgtt ctatcagtat gaatttgatc ggtaagtccg ttggctgcaa actgtcaatg
      721 gacttttttg ctaaaaaaag cgatttctgt tatttatcta ttagaaatag gcttgacatt
      781 tcgtgaaaaa agtttaaaaa tagtacttac ggaaggttgg cagagtggta atgcaccgga
      841 ctcgaaatcc ggcgaaccgg ctaataccgg cgcgcaggtt caaatcctgt accttcctta
      901 tatcaacgat tagaggttgt gaagaagctg tcctgcatca agtaataaga acggccaaac
      961 aaaaatagct tttcctattt tttcgtttga ttgggcttat taccgcagat gcaagctttt
     1021 gaacaccgtt tgttcggaat aaggggctgt gacaagactt ttgtcacagc cccttattgt
     1081 tgtactttta acctacaata ttttttgatt ccccctgagt actatttttt ataaatgcta
     1141 gtaaatcaat cttctttggc ctttttttga taaaaaaaat actagatgta agataaaagt
     1201 gtaaaaacga tttgttttag gaggggaaac gatgtgtacc gcaatcactt atgcaacgaa
     1261 agaccattat tttggaagga attttgatta tgagatgtct tataatgaag tggtcactat
     1321 tactccaaga aattatcgtt tcgattttag aaaggtaaaa aatttagata aacattatgc
     1381 aatgatagga atagcagctg gggtatccaa ctatccactc tattatgagg ctacaaatga
     1441 aaaagggttg agtatggcag gactgaattt tcctggcaat gctgattata aagaattaca
     1501 agaaggaaaa gataacgttg caccatttga gtttattcca tggatactag ggcaatgctc
     1561 aaccatagac gaagctaaag aattattggc tactatcaat ctagtcaata tcgattttag
     1621 tgaaaaacta cccttgtcac ctttacactg gttattagct gataaagaaa aatcgatagt
     1681 aattgaaagt atgaaagatg gacttcattt atatgataat cctgtcggtg tgttgacaaa
     1741 taatccgcca tttgattatc aattatttaa tctgaacaat tatcgctcgc tatcaaatgg
     1801 aa
//
LOCUS       DAJSBL010000154         1796 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.161, whole genome shotgun sequence.
ACCESSION   DAJSBL010000154 DAJSBL010000000
VERSION     DAJSBL010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1796
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.161"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            29..1381
                     /gene="brnQ"
                     /locus_tag="ONT85_002942"
     CDS             29..1381
                     /gene="brnQ"
                     /locus_tag="ONT85_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HCR2379838.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT          503 a          309 c          353 g          631 t
ORIGIN      
        1 ttttaaaaaa accgtaaagg agttatgtat gacaaaaaaa ttatcattta aagactatct
       61 ttttataggc tccatgcttt tcggtctatt ttttggtgct ggaaatctta tctttcccgt
      121 tcatttgggt caagaagcag gcgctgctac cttttgggcg aatctaggtt tcttggttac
      181 cggtatcggt cttcccttct taggggtgat tgccattggt gtatcgaaaa gctcaggtgt
      241 atatgaatta gcaaaacgga taaatcaaac atatgcgatg gtttttacca tcttgcttta
      301 cttagttatc ggaccgtttt ttgctttacc tcgccttgca accacatctt tcgagattgg
      361 tttagcacca tttatcgatc ctagcagaca gacgctatat ctagcaattt tcagtattgt
      421 attttttgtg ctggcttggt ggttttcaag aaaaccgaca aagatattag attatgttgg
      481 aaagttcctg aatcctgcat ttttggtttt attaggtgct ttgcttctcc ttgctttttt
      541 gaatccacta ggttctgtaa atcaagcgcc tattcagcca aattatcaag agcatgcttt
      601 tttcacaggg tttactcaag gatataacac attagatgcg cttgctgctt tagcatttgg
      661 gatcatcatt gtcacaacaa tccaaaacat gggtgtaaca aaacctgctg aaatcgctaa
      721 agacacgatc aaatctggtg cgatcagcat tatattgatg ggaatcattt atactttgct
      781 cgcttattta ggtgctatga gcttaggtag ctttgctcta agtgaaaatg gcgggatcac
      841 gctggctcag attgctgatc attatttagg tacttatggc agtatcttat tagcatttat
      901 cgttatatta gcttgcttga agaccggtat tggtttgatt acagcttttg cagaaacatt
      961 cacagatttg tttccgcaaa gaaattacgc attctttgtt gcacttgcta gtatccttcc
     1021 ttgtttagcg gcaaacgtag gcttgacgaa tatcattcaa ttttcgttgc ctgttttgat
     1081 gttcatctat ccattagcaa tgacactgat tttactagtg ttagttggtc ctttgtttaa
     1141 gcagcgtacg gctgtttatc gaatgactac ttattttaca ttgattgctt cgatttttga
     1201 tgggttgaac gcttgcccag aaactatcaa acaaacacct attgttcaaa atcttttata
     1261 tgttgcggaa agctacttgc cgtttttcaa attggggatg gggtggatcg taccagcagt
     1321 tatcggtttt gttatcggac tgatttggag tttcgctaag aaagaagaag tagcagattg
     1381 ataaaagagg ttgtgaaaga gacgttcagc cttgagcaat aaaaagtaat tttgaaaaat
     1441 ggcttctcac atttttgcat aaatttgact taataccgag aaactgactt ttgaacaccg
     1501 tttattgaga ggtcgtgaaa aagccgctta gtttcaagta ttaagaaata ttttgtgaaa
     1561 atggcttctc acatttttgc ataaatttga cttaataccg agaaactggc ttttgaacac
     1621 cgtttattga gcgtctataa actgacctga gtattagaga aacaaaacgg caaagtagct
     1681 ccatatactt tgccgttttg ttttgaacta ataccgaaga gaagctgatc aaacaacatt
     1741 tcttttataa tatagatttt agacatatat atgatataaa aatagttttc gcaaaa
//
LOCUS       DAJSBL010000155         1729 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.162, whole genome shotgun sequence.
ACCESSION   DAJSBL010000155 DAJSBL010000000
VERSION     DAJSBL010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1729
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.162"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..560
                     /locus_tag="ONT85_002943"
     CDS             <1..560
                     /locus_tag="ONT85_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2379839.1"
                     /translation="KNIKKIPKIDENSKLMKNIQSIFGIEEVFVGDKCIFFDQPLLSD
                     GYGIDETDIFAKVNSIFSGKKETLIKLHPRARTNRYGEKNILNSSLPFEVLLLENDFS
                     STLLISPISTISFSPSMMFEKTINSIMLAKLILNEYTALSEDKKETLTNIVLFCEHYN
                     RLSEEKILLPENWAELRKLVEYGII"
     gene            547..>1729
                     /locus_tag="ONT85_002944"
     CDS             547..>1729
                     /locus_tag="ONT85_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HCR2379840.1"
                     /translation="MGLFNTLSKSKLVKSSFWYTIGNFFIKGISFITIPIFVNITTQA
                     EYGLINNFTSFVSIFSILVGLSLNSAINNASFDYKDDINEFMSSVLSLATLSLIVFLL
                     IGNIYFLFSNAFFDMNQLVFNLMLLQSFASFVISYIQSYFTINFNHFKYLILSFISTF
                     LNIAISVALMQTLYFDDKYVGRVVGGTLSFVLIGLVIYLRIMIKGAHFFDKKQWRYAL
                     KISLPLIPHSLANIVLAQFDRIMINSYLGPAAGGIYSYISNIGIILSVIWMSTNNAWV
                     PWFYGEMSKNNEKKVKKVSNYYLVVFTLITMIVMVVSIDLAKFMAPKDYYSGLSLVVP
                     ITLGYFFQFLYSLPVNAEFYEKKTSFIAIGTVASAIVNILLNMIFIPRLGIIAAGYTT
                     VF"
BASE COUNT          575 a          224 c          276 g          654 t
ORIGIN      
        1 taaaaaatat aaaaaaaatt cccaaaatag atgaaaacag taaattgatg aaaaacatac
       61 aatcaatctt tgggatagaa gaagtttttg tcggagataa atgtatcttt tttgatcaac
      121 ctttattatc tgatggttat ggcatagatg aaacagatat tttcgctaaa gtaaatagta
      181 tattttctgg aaaaaaagaa actttaatta agcttcatcc tagagcaagg acaaatcgtt
      241 atggtgaaaa aaacatattg aattccagtt taccgtttga agtcttgcta cttgaaaatg
      301 acttctcttc aacacttttg attagtccta tatctacaat ttctttttca ccatctatga
      361 tgtttgaaaa gacgatcaac tctattatgt tggctaaatt aattttaaat gaatatacag
      421 cattatctga agataaaaaa gaaacattga caaatattgt tcttttctgt gaacattata
      481 accgtctatc agaagaaaaa attttactcc cagaaaactg ggcagaatta agaaagttgg
      541 tagagtatgg gattatttaa cacactttcc aagtcaaaat tagttaagtc cagtttctgg
      601 tatactatag ggaatttttt tattaaggga atttcattca taactattcc tatttttgta
      661 aatattacta ctcaagcaga atatggtttg attaataatt tcacttcatt tgtatcaatt
      721 ttttcgattc ttgttgggct aagcctaaat tcggcaatta ataatgcaag ttttgactat
      781 aaagatgaca taaatgaatt tatgtcatcg gtactttctc tagctacact ttcgttaatt
      841 gtgttcttat taatagggaa catatatttt ttgttttcaa atgctttttt tgatatgaac
      901 caacttgtat ttaatttgat gttattacaa agttttgcta gttttgtaat tagttatatc
      961 cagtcttatt ttacgattaa ctttaaccat tttaaatatt tgattttatc ttttatatct
     1021 acttttttga atattgcaat ctctgtagct ttgatgcaga cattatattt tgatgataaa
     1081 tatgttggca gagtagtcgg cggaacactg tcatttgtgt tgataggttt agtaatttat
     1141 ttaagaataa tgataaaggg tgctcatttt tttgataaaa aacagtggag atacgcttta
     1201 aaaatctctt tgccgctaat cccacattcc ttagcaaata tagttttagc acaattcgat
     1261 agaattatga ttaattctta tttaggtcct gctgcggggg gaatatattc ctacattagt
     1321 aacataggta ttattctaag cgtaatatgg atgtctacta ataacgcctg ggttccatgg
     1381 ttctatggtg agatgagtaa aaataatgaa aaaaaagtga agaaagttag taactattat
     1441 ttagtagtct ttactttgat tacgatgatt gtaatggtag tttcgattga tttagcaaaa
     1501 tttatggctc cgaaagacta ttattctggg ttatctttag tggttccgat aaccttaggc
     1561 tatttctttc agtttttata tagtttacca gttaatgctg aattttatga gaaaaaaaca
     1621 tcgtttattg cgattggtac agtagcaagt gctatagtta atatactatt aaacatgatt
     1681 ttcataccta ggttaggaat aattgctgct ggatacacaa ccgtattta
//
LOCUS       DAJSBL010000156         1634 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.164, whole genome shotgun sequence.
ACCESSION   DAJSBL010000156 DAJSBL010000000
VERSION     DAJSBL010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1634
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.164"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            101..868
                     /locus_tag="ONT85_002945"
     CDS             101..868
                     /locus_tag="ONT85_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HCR2379841.1"
                     /translation="MKKDELTKYNEEFLATIDMKMDLKSYELYQLLFSYISPTKLKKT
                     GVVDLPVKAIINYMDVDQNLETELKTIVNTLQRQAVYEISNLDKKYKTYTTVSAIDRI
                     HWDLEEHSVSFSFSEDVIPLIVSIKKKYPFFVFEDLASFQKKYSIVLYKYLAMYYKKY
                     LIAKNKGDFEKAEIYKNPLIEIVKIREITKTENEYKTISNFDRRVLRESVEEINEKSM
                     LNIDFAKVRKGRNYNFAVQFNISSEFDEKTYREKKLG"
     gene            1060..1458
                     /locus_tag="ONT85_002946"
     CDS             1060..1458
                     /locus_tag="ONT85_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HCR2379842.1"
                     /translation="MKYFFCAVFEWKGNEYAVKFPDFPDLYTHGETLEDAFEMAQEVL
                     EGYLVLLELQEKEIPSPTPVQELNARIKRSENVNLFVVKANTKVAKARNKQNYDRKSV
                     TVEHYLNVLAAEENINLSKALNDLLREKLI"
BASE COUNT          592 a          212 c          315 g          515 t
ORIGIN      
        1 aatactgtta aatatctcga aaaatcaatt acgagttatt tgccttatgt gaacctttat
       61 gattaacaac tgaaaaaatg cgtgaaggaa ggaaaaactt ttgaaaaaag atgaactgac
      121 aaagtacaat gaggagttct tagcaacaat tgacatgaaa atggatttaa agtcgtatga
      181 actttatcaa ttgctgttca gttatatctc acccacaaag ctaaagaaaa caggcgtagt
      241 agatttacct gttaaagcga ttattaatta catggatgtt gatcaaaatc ttgaaacgga
      301 actaaaaacc atagttaaca ctttgcaaag acaagcagtt tatgaaattt cgaatctaga
      361 caaaaaatat aagacgtata ctactgtatc tgctattgat cggattcatt gggatttaga
      421 agagcatagt gtatctttca gtttttcaga agatgtaatt cccttaattg tctcaatcaa
      481 aaagaagtat ccgttctttg tttttgaaga cttagcatct tttcaaaaaa aatatagcat
      541 cgtgttatac aaatatttgg cgatgtatta taaaaaatat cttatcgcca aaaataaagg
      601 agattttgag aaagctgaaa tatataagaa tcccctgatt gaaatagtta aaattcgtga
      661 gattacgaaa acagaaaatg agtataaaac aatatcgaat tttgatagac gtgttttaag
      721 ggaatcagtt gaggaaatta atgaaaaatc aatgctgaat attgattttg ctaaagttag
      781 aaaaggcaga aattacaatt ttgctgtcca gtttaatatt tctagtgagt ttgatgaaaa
      841 aacttataga gaaaaaaaat taggttaatg caaaatatat cttactcgtt tgaagatgct
      901 tcagacgtat gctaagatat atagtgcctt gggagcaagg tattaatctc ctttagaggt
      961 taattagctt gtgatcgcct atcaattttt ttggtaggcg attctttttt ttgaaacaaa
     1021 aaagcaaaaa ttatttttta attagagagg tgcaatgcga tgaaatactt tttttgtgca
     1081 gtattcgaat ggaaaggaaa tgaatatgca gttaaattcc cagattttcc agacttatat
     1141 acacatggtg aaactttaga agatgctttt gagatggcgc aagaagtatt ggaaggatac
     1201 ttggtgcttt tagagttgca agaaaaagaa attccatcgc ctacgcctgt tcaagagtta
     1261 aatgcaagaa tcaagcgatc tgagaatgtt aatttatttg tcgtaaaagc taatacgaaa
     1321 gttgctaaag ctcgaaacaa gcaaaattat gatcgaaaat cagtgactgt ggaacactat
     1381 ttaaatgttt tagcggctga agagaatatt aatctgtcga aagctttgaa tgatttgttg
     1441 agagaaaagt tgatttgatg tctctcagat gatcatatta agcaaaaaca gcttgtttta
     1501 ggacgtatgg atttaacaga attaggcaga agtcgcccat cctctatagg tgggtgatga
     1561 atgccgttcg gtatgagggt taccgttggt aactcgaaga ccgacaacta aggcgtattg
     1621 cctttttatt ttgt
//
LOCUS       DAJSBL010000157         1577 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.166, whole genome shotgun sequence.
ACCESSION   DAJSBL010000157 DAJSBL010000000
VERSION     DAJSBL010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1577
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.166"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            138..752
                     /locus_tag="ONT85_002947"
     CDS             138..752
                     /locus_tag="ONT85_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR2379843.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDFVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANAKDPQ
                     RRLVYKNIVEDLKKGVAIAKIAKDYNVTRQTVYRIKKDSMVNHE"
     gene            complement(776..1126)
                     /locus_tag="ONT85_002948"
     CDS             complement(776..1126)
                     /locus_tag="ONT85_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HCR2379844.1"
                     /translation="MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVV
                     TVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLTTIDYEARECIFLEKAHDSLVEE
                     LLMKVRTVFQKVSK"
     gene            complement(1120..1383)
                     /locus_tag="ONT85_002949"
     CDS             complement(1120..1383)
                     /locus_tag="ONT85_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PbsX family transcriptional regulator"
                     /protein_id="HCR2379845.1"
                     /translation="MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQK
                     RLIIEAANIENELTIEQLFENYTDERNHVTIQNLGEAVGNEKW"
BASE COUNT          520 a          286 c          245 g          526 t
ORIGIN      
        1 atactatttc ctccatcatt agtttttgtc tatataaggt ataccctaac tagacacgac
       61 cgcgacattg aaaaatctgg ttttctcgtg tatgattagg gtgtttttta aatagttgca
      121 gaaagtaggt aaaaattttg agagttgcat acatacgtgt ctcatccatc gatcaaaatg
      181 aacaacggca gattgaagaa atgaaaaaat tcggtgcaga gagaattttc attgagaaac
      241 aatctggcgc aacgattact catcgacctg tttttcaaga agcactggat ttcgtccgag
      301 atcaagatat ttttattgtg gaagctattg atcgacttgg aagaaattac gacgagatca
      361 tcgcttcagt caattacttg aagaagaaaa atgtgcagct gatcatcaca agtctaccga
      421 tcatggccga agcaattggc aatcctctcc tggataaatt cattaaagat ttgatcattc
      481 aaattttggc catgatcgct gaacaagaac gtaccgaatc taaacgacgt caggcacaag
      541 gcattaagat tgcaaaagcg aacggtgttt ataaaggacg tcctaagcta tacagtgcca
      601 atgcaaaaga tccccaacgt cgtcttgttt ataaaaacat cgttgaggat ctaaaaaaag
      661 gtgttgcgat cgctaagatt gcaaaagact acaatgttac gagacaaacc gtatatagaa
      721 taaaaaaaga cagcatggtc aatcacgaat agttgatcat gctgctgttt ctattttatt
      781 tgcttacttt ttgaaaaact gttctaactt tcatcaataa ctcctcaact aaactatcat
      841 gcgctttttc caaaaaaata cactctctgg cttcataatc aattgtcgtt agttgatcta
      901 ataatacttt tcctgttgtc ttcatttcat attctgagtt tatatttaca tatagcggat
      961 agtttctagt tgtactagaa attggagcaa ctgtaacaac gttactataa tccgcaataa
     1021 tatcatgact taaaacaata taaggtcttt tacctgtctg ttcgtgtcca gatcgaggtg
     1081 ctgtattcaa taatagaata tcgccttgtc taggttttct caccattttt cattacctac
     1141 tgcttcacct aaattttgaa tcgtcacatg atttctttca tcagtgtaat tttcaaataa
     1201 ctgttcaatt gttaattcat tttctatatt agccgcttcg ataattagcc gcttctgacc
     1261 gtttacttct tcttgtatta tcttcacttt gtcttctgta tgaattccaa gatattccat
     1321 aatatacttt ggcaatctaa ggccattact atttccccat ttttttatag gtaacatttc
     1381 catagtagac acctctttct gtatatacat agtataacat acataagaaa aaacgtatat
     1441 acttaattat ccttttctat taaattagat taacattcca tgaatccata aactatgtta
     1501 acatttatat gaaaatagtc gaaaggaaat cataatatgt ttaagaaaac aaaatcttta
     1561 gaatcaggta agaaaaa
//
LOCUS       DAJSBL010000158         1573 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.167, whole genome shotgun sequence.
ACCESSION   DAJSBL010000158 DAJSBL010000000
VERSION     DAJSBL010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1573
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.167"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
BASE COUNT          594 a          179 c          320 g          480 t
ORIGIN      
        1 attatcgaac acctttggaa gtatttttga gttacgtaag tattgatgat ctgtctaatt
       61 taatttgaca attaagaaag tattaacttg ttttgtgaaa aagagaattt gaaaaacgat
      121 tggcgaacaa atgctcggta ggtatggtat aaatacagag ctaattggtg tgatgaaatc
      181 agatattcgc gtgagattca gtaaagtagg agttcgaaaa cagctaggtg acaaacagta
      241 ttgctttggt acttcggaat ggtttatcaa attcttatca acagttcact tctctttatg
      301 tttgagtgat gattcgttag gttattgctt ggaaagaccg gatgagagta gaagagttta
      361 attttataaa gtgaaaagag gtaatgtacg atatacgtag ggctgaacca atagaggata
      421 agcaaacgat gttacaggcg caaaattcat agcttactgt tttggagata acagaagagg
      481 aaataatgca gaagtaaggc gaaaaaagga cggaagccta acagcatatg aaataaagaa
      541 aaatattata actactgata aatcggtatt gagcaaagta ctaataggag tcaaaaacat
      601 tcataagcta tgagtgtttc ttggctactt ttttattttt atgtatctac aaaagtttcc
      661 aggggcagaa cctgtgatta ttaatggaag aacctcaagt attgtcttaa atcattgtta
      721 tgaaaatgga ttaaaagagt tcatcaaggt gtgattaaca agttactttt cattacatcg
      781 aatttttcag aagttgctta tattggtgat gatattaatg atttatcatg tatgacatct
      841 gtgaagaagc gggaggaata gtagaatgtc caaaaaatgc cacaagaaat gagattgaat
      901 tatctgattt tattgtaact cacagtggtg gtgacgaatc tgtgatagag tttattgagt
      961 ggatattgga atcgatatag tgtagtagta cagaaaaatt atcatacaat taatactagg
     1021 cataagaata ttggcatatt ctagagttaa aagtaactat tcgaaaagga taaggttaga
     1081 attttttgtt ttgccagtgg aagaatataa aaagggcatt ggaaattaat atattagaat
     1141 gctaagcgga gaaaaaaata tatgaaaagt acgtccttgt gatgggatgg aaaaatacac
     1201 ttatttcaaa caacgataat ataattatgt gaaccgaagt atactcgctt ctaattcaat
     1261 cctcaaatcc cgacagtcgc gacttcaaaa aagcgtatag ttttaatgta tccccatatg
     1321 atccgaaaaa tggtaattta caattaacag tttgatgatg ttgaagatgt ggaaatgctg
     1381 aaattaccat gatgagaaac acattaatta aagggaatta attaactaga atataatctg
     1441 attcaatttg aattaagcaa aaaaaggctg agttttaaat aatcgcgaat tgtaaaatta
     1501 agttagaaaa ataaaaaggc atttatggta cactcaaatt gtatttccga cgaaagaaaa
     1561 caaaggagtg taa
//
LOCUS       DAJSBL010000159         1498 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.169, whole genome shotgun sequence.
ACCESSION   DAJSBL010000159 DAJSBL010000000
VERSION     DAJSBL010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1498
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.169"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..626)
                     /locus_tag="ONT85_002950"
     CDS             complement(<1..626)
                     /locus_tag="ONT85_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR2379846.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGRLDDSP
                     SHCEHCGSIRIIRNGSYTTRTQILKVKEKLTILELKRTRFLCYDCGQTFSAKTDLVDE
                     HHQLTKELKQAILIELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSMKSVSGSMSFICVDGTTHQLFTILEDRRLYKLTQYFLRFPR"
     gene            914..>1498
                     /locus_tag="ONT85_002951"
     CDS             914..>1498
                     /locus_tag="ONT85_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR2379847.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKEL"
BASE COUNT          521 a          217 c          285 g          475 t
ORIGIN      
        1 tttctaggaa agcgcaggaa gtattgagtt agtttgtaaa gtctgcgatc ttcaaggatc
       61 gtaaatagct gatgggttgt cccatcaaca caaataaaac tcatggaacc agaaacagac
      121 ttcattgatt tgaactcatc catacacaac accgtcggaa ggaagttcat acgtggctgg
      181 taatgcttcg ttgcttcacg caagatccgt gtcaccgtcc catccgagac gaagtacttc
      241 ttagcgatta gtttacgtga ttgattttcg tacagttcta tgagaatcgc ctgcttcagt
      301 tcctttgtta gttgatgatg ttcatcaact aagtccgttt tcgcagaaaa ggtctgccca
      361 caatcatagc atagaaaacg ggtacgtttt aattctaaaa tagtcaactt ttctttgact
      421 ttcaatatct gagtacgtgt cgtataggaa ccgttgcgga taatacgaat cgatccacag
      481 tgttcacaat gggaaggtga gtcatccaat ctccctttaa taaactgcgc ttgaacgcct
      541 ttatattctt tttcaatcag ccattcttca tcaaaaaata aatgtttatc tgttaatccg
      601 agtaattttc tagtatgatt atccatagag cgactccttt ggtagggtga tgttttggtc
      661 gactaaattt taccaaaagt gagtctcttt ttgagttgaa acgaagaaaa agtgttagta
      721 gaaaatcgcc ttcgattttc caacaacaca aaatattata gaacccttta ttctaaagga
      781 tttcaaggtg tctgtttgca tttattagaa aaaagaaaaa gtattggcaa cagaaggtgt
      841 caccaacact agatattata gagccataat atgctgtata atatctagtg tataggctta
      901 agtggaggta tatatgaaaa tattaactgt tgaagatgat aatatgataa gagagggaat
      961 aagtgaatat ctttcagaat ttggatatac tgttattcaa gctaaagatg gaagagaagc
     1021 cttgtcaaaa tttaatagcg atataaattt ggttatctta gacattcaga taccttttat
     1081 aaatggttta gaagtgttga aagaaattag aaagaaaagc aatttaccaa ttctaatctt
     1141 gactgcattt agtgatgaag aatataaaat tgatgcattt actaatttag tagacggata
     1201 tgtagaaaag ccattttcat tgccggtctt aaaagctaga atagattctt taattaagaa
     1261 gaattatgga catttagaga agtttgaata taaggatcta tcggttaatt ttaatagcta
     1321 tacagctaaa ataaatggtg aagaaataga tgtaaatgca aaagaactag aagtattaaa
     1381 atgtttattg gataataatg gacaagtgtt aacaagaatg caaattattg attatgtatg
     1441 gaaagatagc gaagaaactc cttatgatcg agtaatagat gtatatataa aagaactt
//
LOCUS       DAJSBL010000160         1495 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.170, whole genome shotgun sequence.
ACCESSION   DAJSBL010000160 DAJSBL010000000
VERSION     DAJSBL010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1495
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.170"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(522..878)
                     /locus_tag="ONT85_002952"
     CDS             complement(522..878)
                     /locus_tag="ONT85_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HCR2379848.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
     gene            complement(892..1302)
                     /gene="spx"
                     /locus_tag="ONT85_002953"
     CDS             complement(892..1302)
                     /gene="spx"
                     /locus_tag="ONT85_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HCR2379849.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
BASE COUNT          468 a          265 c          213 g          549 t
ORIGIN      
        1 gagtgtttcc cgattcgttt ttggctgccg tagcaacttt tatatcatat cagagagaca
       61 agcgattgtc aactcttttt taaaagtttt tttcgatttc gtttttcgaa atctttcagc
      121 aactcattta tcataactta tcgtttgtta tttgtcaaga acttttttga agtttttttc
      181 gatagaatca agcttcgcaa gtggttcttt tttaacaact cagttatctt atcatgtcat
      241 ttctaacatg tcaacaacta ttttatatgt tttttaaaat actattttat tgctttgcta
      301 cttaaaacaa cagaaatgaa tatatcatgt aaatttacac atgtcaatat atttttaact
      361 ctatatcccc acctagattt gaattttgac cacataaatt gagcatgtct ctatagtaga
      421 aaaataaagt caaaaaaaga acacataaca cgcttgatgc taagtgttcg actgttacaa
      481 ctgtatgaaa tgctcagtga cttgattaaa acagttaaag tctatttatt taagataagc
      541 tgttccatca actgctcatt gtatccaata aatagaactt catcgataat aatcaaagga
      601 cgtctgagga gattggtatg ttgtgtgcat aaaaaaatca gttgtttttt tcctacacag
      661 gaaaggtcaa tcgcaatgtt gtgaaaagcc tcagaccaag tcgcaaatag ctgttctgct
      721 tgcacttctt caaaactgca taattcttct gcttcttctg tacttagatc gcgttcttct
      781 aagtcaacaa attgataagg caattgatgg tgttctaacc attgtttcgc cttcagactt
      841 gttacacagt gcgctgatcc atagactttc acttccacga aggctccctc cctatgaatt
      901 taagatgtta ttgttttgta aatagatcag atttctggca acacatcgaa tttcacgagg
      961 gacaaatgac cggatctcat cttcattaaa tccgattagt attctttttt catcaatcaa
     1021 aataggtcgt ctcaataatg aaggatattc tttgaataag acgatcattt gctttaaagt
     1081 taattcgtta aaatctatgc ccagcttttc gtaaattttc gaccgagtgg aaataatatc
     1141 ttgaactcct ccttcagtcg ctgccaagat ctttttgatc tcactttctg ttaacggttc
     1201 ggcaaaaata ttcttctctt caaattcaag ttgattcgct tgcaaccatg ctcttgcttt
     1261 tctacaggaa gtacagctag gcgaagtata tacttttatc attttctcac tcatttttaa
     1321 ttataatcca attctacagt aatttctttt aaaataccaa ttattagcta ataatatata
     1381 aaaaaaaatc aaaataactg attatttacg tttgaataat gagaattttt ctatatattc
     1441 tctattttga taatagtatt ctcattttca aaataaataa gggaggtgtg acaaa
//
LOCUS       DAJSBL010000161         1454 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.171, whole genome shotgun sequence.
ACCESSION   DAJSBL010000161 DAJSBL010000000
VERSION     DAJSBL010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1454
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.171"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..625)
                     /locus_tag="ONT85_002954"
     CDS             complement(<1..625)
                     /locus_tag="ONT85_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HCR2379850.1"
                     /translation="MKTINLIKVFIASPGDIAAKRNEVEETIYRWNIENTDNTKVILM
                     PIRWETNAIPTYINNSSGQSVINKQIVENSDILLAIFGHKLGSPVDNYRSGTIAEIDY
                     FYKNSKGEVGVFFLDEPVPQVLAEEYIEVKKYRDSLEDKGFYGTYSKDNIRRFLTKKV
                     NYLIEKAQNNLESKRFDLDSISYKTPSVINKKVTGTDKDFDDNLDIFD"
     gene            complement(784..1050)
                     /locus_tag="ONT85_002955"
     CDS             complement(784..1050)
                     /locus_tag="ONT85_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR2379851.1"
                     /translation="MMIKKLIEEKQKDKDFAEAYDREGRKLETAVALYHAREEAGLTQ
                     AELAQKAHTTQSTIARIERGDNVSFDKLDDIARALGKKLVVEFA"
     gene            complement(1040..1396)
                     /locus_tag="ONT85_002956"
     CDS             complement(1040..1396)
                     /locus_tag="ONT85_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HCR2379852.1"
                     /translation="MEKISFETFKRPNGHDEFLEWLETLPKKDSAKLLRTIEETEKNG
                     MLVAQRLKWVKKLDTNLYELRSKVGSNIQRAIYFHVVNGRYVITHGFTKKTEKTPISE
                     LKHAKELREEWFTNDD"
BASE COUNT          406 a          279 c          181 g          588 t
ORIGIN      
        1 gatcaaaaat atctaagttg tcatcaaaat ctttatcagt tccggtaact tttttgttaa
       61 ttactgaagg tgtcttgtaa gaaatagaat ctaaatcaaa gcgttttgat tctaaattat
      121 tttgtgcttt ctcaattaaa taattcactt tttttgtgag aaaccttctg atattgtctt
      181 tgctatatgt tccataaaaa cccttatctt ctaaagagtc cctgtacttc ttaacttcta
      241 tatactcttc agccaaaact tgtggaactg gttcatctaa gaaaaaaacc cctacctcgc
      301 ctttgctatt tttataaaag taatcaattt ctgctatagt tcccgatcta taattatcaa
      361 ctggagaacc caacttatgg ccaaaaatcg ctaataatat atcactattt tctacaattt
      421 gtttattaat aacactttgc ccagagctat tatttatata agtaggaatc gcattcgttt
      481 cccatcgaat tggcatcaaa attacttttg tattatctgt attttctata ttccatctat
      541 atatggtttc ttctacttca ttcctctttg ctgctatatc acctggtgat gctataaata
      601 cttttattag atttatggtt ttcatttatt gtacctttct ctttatgtct ctttaaagta
      661 atttttgtat aaaatttatt gacttttaca ctataggaca acttttatct atttagtaca
      721 aaaaggccca ctaaactaat tgattagtta ggagcctatt acaggtatat tttcaactcg
      781 cttttaagca aattctacta ccagcttttt gcctaatgcc ctagcgatat catcaagttt
      841 atcgaaagat acgttatccc ctctctcaat tcgagcaata gtagattgag ttgtatgtgc
      901 tttttgtgca agctccgctt gagttaggcc tgcttcctcc cgtgcatgat aaagagcaac
      961 cgcagtttcg agttttcttc cttcacggtc atatgcttct gcaaaatctt tgtctttttg
     1021 cttttcttca atcaattttt taatcatcat ttgtgaacca ctcctctcta agttcttttg
     1081 catgtttaag ttcacttatt ggtgttttct ccgttttttt agtaaatcca tgggtaatga
     1141 cataccttcc attgaccaca tggaaataga tagctctttg gatgtttgat cctaccttac
     1201 ttcttaattc gtacaaatta gtgtctaatt ttttcaccca tttcaaccgt tgagctacta
     1261 gcatcccatt cttttctgtt tcttctattg tacgtaaaag cttcgcacta tctttttttg
     1321 gtaatgtttc caaccattct aaaaattcat catgaccatt aggtctttta aatgtttcaa
     1381 aagatatttt ttccataccc aattatatca tatattgatt ttatattata gtatataaca
     1441 taaaaattcg aatt
//
LOCUS       DAJSBL010000162         1453 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.172, whole genome shotgun sequence.
ACCESSION   DAJSBL010000162 DAJSBL010000000
VERSION     DAJSBL010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1453
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.172"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..867
                     /locus_tag="ONT85_002957"
     CDS             <1..867
                     /locus_tag="ONT85_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HCR2379853.1"
                     /translation="VGDKKVQFTFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFG
                     RRSADGLQRERFVLFNQFKIDGKADIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNEL
                     ESSYSKTMFRLLKQYRTKGIAYFSKEDFHELLDIPKSYRESDINKKIIKPIRQELTAI
                     FKGLTIKKKYGKGRGKPVIGYQFTFKPEIKKADDFNKHAPTKEEQNKALELVNQQIYS
                     EKDPTSLQEKIKEQKRKLELLQQLEELERKQNNLNKENKEINEQIPDNNSDIPPELKK
                     GLLDNLQNLFKE"
     gene            877..1374
                     /locus_tag="ONT85_002958"
     CDS             877..1374
                     /locus_tag="ONT85_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR2379854.1"
                     /translation="MTDKTIKELADILGVSKDKVKYQLRKLPSNFTYKKGNIIYLKKE
                     AVTTITKVLSGNEPGNLPSELPDSLPTDYLLKEIETKNNHIEEKDRQIEKLQLALETQ
                     QKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENKSTTLSEETESIEKQSKTEKKW
                     WQFWQ"
BASE COUNT          629 a          236 c          222 g          366 t
ORIGIN      
        1 gtaggcgata aaaaagtaca atttacattc gaacaattaa aagatttaag caattataaa
       61 gcaactgcaa atgttcgatt catagatgat ttagaaacaa cgtatgacaa attaatggat
      121 ttacgttttg gtagaagaag tgctgacggt ttgcaacgtg aacgatttgt tttatttaat
      181 caatttaaaa tcgatggaaa agctgatatt ccttttgctg aaatacaagt ccatgagaaa
      241 gccttaccct tacttaataa tcttgaagaa tgggttcgat attctctcca acaattcaat
      301 gaattagaaa gcagttattc caaaaccatg tttagattat taaaacaata cagaaccaaa
      361 ggaattgcct atttttcaaa agaagacttt catgaattac ttgatatccc taaaagttat
      421 agagaaagtg atataaataa aaaaattata aagcctattc gccaagaact aaccgcaatc
      481 ttcaaaggtc tgacaatcaa aaagaaatac ggaaaaggaa gaggaaaacc tgtcattggc
      541 tatcaattca cgtttaaacc tgaaataaaa aaagcagacg actttaataa acacgcacca
      601 acaaaagaag aacaaaataa agctttagaa ctagttaatc aacaaattta ctctgaaaaa
      661 gaccccactt cattacaaga aaaaatcaaa gaacagaaac gaaaattaga acttttacaa
      721 caattagaag agctagaaag aaaacaaaat aatctgaata aagaaaataa ggagattaac
      781 gaacaaatac cagataacaa ttccgatatt cccccagaat taaaaaaagg cttactagat
      841 aatcttcaaa acttatttaa agaataaggg gtttttatga cagataaaac tatcaaagaa
      901 ttagcagata ttctaggtgt ttcaaaagat aaagtgaagt atcaactgag gaaattacct
      961 agtaatttta cctacaaaaa aggaaatatt atctacctga aaaaagaagc agtaacaaca
     1021 attacaaagg ttttatcagg taatgagccg ggtaacttac ctagtgaatt acctgattca
     1081 ttacctaccg attacctact caaggagatt gaaactaaga ataatcatat tgaagaaaaa
     1141 gataggcaaa ttgaaaaact tcaactagct ttagaaacac aacaaaaatt actcgaccag
     1201 caacaacaac tcaccctgca agccaaccaa caaatcgaga aactgcagga acaactacag
     1261 ttgacttata cggaagaatc ttcggaaaat aaatcaacga ctctttctga agaaacagaa
     1321 tcgatagaaa aacaatcaaa gacggagaaa aaatggtggc aattctggca ataaaaccag
     1381 caatttaaat taataaaaat tgctggtttt attcttcttt ttgcttttgc gagcagagca
     1441 acatgggccc atg
//
LOCUS       DAJSBL010000163         1435 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.173, whole genome shotgun sequence.
ACCESSION   DAJSBL010000163 DAJSBL010000000
VERSION     DAJSBL010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1435
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.173"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            105..1406
                     /locus_tag="ONT85_002959"
     CDS             105..1406
                     /locus_tag="ONT85_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HCR2379855.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
BASE COUNT          473 a          287 c          264 g          411 t
ORIGIN      
        1 gatagaaaat cattctatcg gtcccttttg agcatgaaaa aacaaaacat acagaagctc
       61 cgttagaatt gaatcaccac aacacaatca aaggagcgtt ctgtatgcct gatcttcatc
      121 atatcaaact actacttgga atcaaagata aaaacatttt catcacaaat gtggaatcga
      181 agagcattaa gaatatcaag agtcttgttg tttctgcgac gttaagtaaa gaaattcgtc
      241 gctgtccttt gtgtaaacag atgaatcatg aaggcatgat cgttaagaac ggaaagaaaa
      301 aatcgctcat tcaacttaat aaatgtgcga accaactgac ttatcttgct ttagcgaaac
      361 aacgctacca ctgtcgtggc tgccacacct attttacggc gaatacctac attgtcgata
      421 gaaactgctt tatcgctaaa caagtgcgct ataaaatttt agaagagcta actgaaaaac
      481 aagcaatgac gacaattgct aagcattgcg gcgtttcttg gtcgaccgtt tctcggacac
      541 tcgcttctct gatccccatg acaaaagtaa aaagaaattg gttaccgcgt tgtttattaa
      601 tggatgaatt tcgttcactg aaaaatcaag tcggtcccta ctccttttct tgtatggatg
      661 gcgatactgg aaaattactg gatattcttc cttcacggaa gaaaaaggct ctcgtttctt
      721 attttatgca atttgaacga cgcgcacgat tgaaggtaaa ggtcatcgtg acggacatga
      781 atgtttctta tgcctctctg ataaaggaat gttttccaaa ggcaaaactc gttgtggatc
      841 gttttcacat tgtcaaacat ctcattcgta aatttgagga tattcgcgta cgaatcatga
      901 aaagctttga tcgaaacgat ccaacacaag ccaaatatta tcgacaatta aaggcgttgt
      961 ctcggcttct gatcaaacga caggacaagc ttgtttatga caaatggacc aaatggcgca
     1021 attttggttg ggcgtatctc accgaaagtg aagtggtgga tcgtctgctt tcgatctctg
     1081 acgaattacg aatcgcctat ttttactatc aagaaattct tcaagcgttt cacgacaaag
     1141 aggcggacac tttctttaaa cttgtaagaa cgatgcccaa ttccgttcca aaagaactgc
     1201 attctataaa aaaagctttt ataaactatg aatccggcat tcgtttagcc ctcgaattgc
     1261 cttattcaaa tgccaaaata gaaaatctgc atacccacat caaagcattg aaacgagtcg
     1321 cttatggctt tcgaagcttt cggaaaatga agaccagaat tttcttacta aataatctga
     1381 ttacctatga atcaaaaaac atctaaagta gaaaaggaat tctaccttag atgtt
//
LOCUS       DAJSBL010000164         1417 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.174, whole genome shotgun sequence.
ACCESSION   DAJSBL010000164 DAJSBL010000000
VERSION     DAJSBL010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1417
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.174"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(150..1337)
                     /locus_tag="ONT85_002960"
     CDS             complement(150..1337)
                     /locus_tag="ONT85_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HCR2379856.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
BASE COUNT          412 a          308 c          249 g          448 t
ORIGIN      
        1 taaaagttca acctcatcaa aagcacaatg ttttgcgttg tgaacctaac tgccatgcct
       61 ttctttctga ggagagaggt caggagcagt agcgcagcga ctcttgatgt ctctcctcag
      121 aaagaagaga ctcgcacagg ttcatggtgc taaatgttat tcgaattaaa ttctaaatat
      181 tttcgtggag aagccagcca ctcagtgtct ttttccatca aaagtgttcc aattatccga
      241 gtgatggaat tgatatttgg aaaaatccga atcactcgtt ctctccggcg aatctcttcg
      301 ttcaaccgtt ccaacacatt ggttgtacga atccttcggc ggatattttc aggaaaagac
      361 aaaatttgaa tagcatcttc aaagccgttt tctaaaatgt cgcaagcagc acttaatttt
      421 gaatcacaac catatttttc caagaagtgc tcttttcttt cacgtgtcaa ttcaagttca
      481 gaggctttaa agatactctt caactcgtct tttacatcag aagaaacttt ctttggcagc
      541 ttatcaaaaa tatttctcaa aaagtgggct tggcatcttt gccagctcgc acctaaaaag
      601 ttcttgcgaa cagccttaac taaccctttg tgtgcatcag aaatgaccag ttttacaccg
      661 aacaaacctc gttgaacgaa cgattgaaaa acggtatccc aattatcttc cgattcgccg
      721 tcttgaacat caaaaccaag aatcgtacgt cgtccatcag aatcaatgcc gataccaata
      781 tatacacctt ttgagacaat acgatgattt tcccttactt tcatatacag ggcatcacac
      841 atgaaaaatg gatattccga gccttctagg ctccggtttc tccactcaaa aacaaatgga
      901 tccaactgtt tcatcagatt tgaaacgaat gatttagaaa cagtcgcacc atccgttagc
      961 agttcaactg ttttagttac tttgcgagtg gagacaccag aaatgaccat ttcaatcatg
     1021 gcagcgatca aagctttttc gcttcgctga tatctttcaa agatagtagg actaaactta
     1081 ccgtctctgg ttcgtggaac atgaagttct aacgtaccaa ttcgagttgt aaacgaacga
     1141 tcgtagtaac catttctttg tgattgccgt tcttctgaac gttcataggc atttgcctga
     1201 atatattgat ttcgttgttc ttccatcagc tgattaaaca tgatagtcat aagtgttttt
     1261 gcggtatcat tcgcaccgct ttcgtttata attgcttgaa tttcttcgct ttcaaaagta
     1321 aaatgtactt gagtcattga tatcagtcct attcgagtat gttttctgca aaaaacattg
     1381 tactcctaac tgagatcaat ggcttttttg atgcttc
//
LOCUS       DAJSBL010000165         1388 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.175, whole genome shotgun sequence.
ACCESSION   DAJSBL010000165 DAJSBL010000000
VERSION     DAJSBL010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1388
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.175"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            1..1235
                     /gene="aph(2'')-I"
                     /locus_tag="ONT85_002961"
                     /pseudo
     CDS             1..1235
                     /gene="aph(2'')-I"
                     /locus_tag="ONT85_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="APH(2'')-Ia/If/Ih family aminoglycoside
                     O-phosphotransferase"
     gene            1219..>1388
                     /locus_tag="ONT85_002962"
     CDS             1219..>1388
                     /locus_tag="ONT85_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR2379857.1"
                     /translation="MKLLFIDDNMVFDIDNLKGFLNDTSSFGFIAKENNKIIGFAYCY
                     TLLRPDGKTMFY"
BASE COUNT          571 a          110 c          235 g          472 t
ORIGIN      
        1 aatcattaaa aaaacattat acagagcctt gggaagatga agtttttaga gtaattattg
       61 aatataacaa tgttcctatt ggatatggac aaatatataa aatgtatgat gagttatata
      121 ctgattatca ttatccaaaa actgatgaga tagtctatgg tatggatcaa tttataggag
      181 agccaaatta ttggagtaaa ggaattggta caagatatat taaattgatt tttgaatttt
      241 tgaaaaaaga aagaaatgct aatgcagtta ttttagaccc tcataaaaat aatccaagag
      301 caataagggc ataccaaaaa tctggtttta gaattattga agatttgcca gaacatgaat
      361 tacacgaggg caaaaaagaa gattgttatt taatggaata tagatatgat gataatgcca
      421 caaatgttaa ggcaatgaaa tatttaattg agcattactt tgataatttc aaagtagata
      481 gtattgaaat aatcggtagt ggttatgata gtgtggcata tttagttaat aatgaataca
      541 tttttaaaac aaaatttagt actaataaga aaaaaggtta tgcaaaagaa aaagcaatat
      601 ataatttttt aaatacaaat ttagaaacta atgtaaaaat tcctaatatt gaatattcgt
      661 atattagtga tgaattatct atactaggtt ataaagaaat taaaggaact tttttaacac
      721 cagaaattta ttctactatg tcagaagaag aacaaaattt gttaaaacga gatattgcca
      781 gttttttaag acaaatgcac ggtttagatt atacagatat tagtgaatgt actattgata
      841 ataaacaaaa tgtattagaa gagtatatat tgttgcgtga aactatttat aatgatttaa
      901 ctgatataga aaaagattat atagaaagtt ttatggaaag actaaatgca acaacagttt
      961 ttgagggtaa aaagtgttta tgccataatg attttagttg taatcatcta ttgttagatg
     1021 gcaataatag attaactgga ataattgatt ttggagattc tggaattata gatgaatatt
     1081 gtgattttat atacttactt gaagatagtg aagaagaaat aggaacaaat tttggagaag
     1141 atatattaag aatgtatgga aatatagata ttgagaaagc aaaagaatat caagatatag
     1201 ttgaagaata ttatcctatt gaaactattg tttattgatg ataatatggt ttttgatatt
     1261 gataatttaa aaggatttct taatgatacc agttcatttg ggtttatagc taaagaaaat
     1321 aataaaatta taggatttgc atattgctat acacttttaa gacctgatgg aaaaacaatg
     1381 ttttattt
//
LOCUS       DAJSBL010000166         1374 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.177, whole genome shotgun sequence.
ACCESSION   DAJSBL010000166 DAJSBL010000000
VERSION     DAJSBL010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1374
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.177"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            33..1372
                     /locus_tag="ONT85_002963"
     CDS             join(33..474,474..1372)
                     /locus_tag="ONT85_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HCR2379858.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRAENAYFKKVRNLDSRTRF
                     EQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYYWVKKLDKPDKYSKIKQEITAIVK
                     ESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMGLTCQIRIKRYKSYKGTVGKIAKN
                     VLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPILDLFNGEIISYSISTSPTYKLIEE
                     MLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLKENNIIQSMSRKGNCLDNSVIENF
                     FGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKRIKLKLNGLSPVEYRKQSIK"
BASE COUNT          576 a          177 c          239 g          382 t
ORIGIN      
        1 gactagaaat ctaattaaaa ggggtgcttt ttttgcggaa atatacattt gtttttaaga
       61 aaaaagtagt ttcagactat ttaaataacg aaggcggcta caaatatctt gcacataaat
      121 atcaaataaa tcgtacactg gttagacatt gggtaaggat ttataactat catggttggg
      181 aaggcttggt tggaggtggc aaaagctaca ctacaaaatt taaacttgat gttatagaat
      241 atatggaaac aaatggtctt tctatccaag aaactgctaa aaaatttaat atcggttcaa
      301 atagaactct aagtaaatgg atagagcaat atgaagaagg cggtgcttct tcacttgaga
      361 gccaaaaaag gggcagaaaa attagtatga attccaagct aaacattcct aaaaaactta
      421 aagatgagtc tcttgaagaa gaagttattc gtttaagagc agagaacgca tatttaaaaa
      481 agttagaaac cttgattcaa gaacaagatt tgagcaagaa gaaatcaagg ttaaaacaat
      541 ccttcagctc aaaaaagaat ttaaattgaa tctattactt tcaattgcac aattagcgaa
      601 gtcaacttat tattactggg taaaaaaatt agataagcca gataaatata gcaagataaa
      661 gcaagaaatt acagcgattg taaaagaatc aagaaactct tatggttatc gtagagtcac
      721 tttagcgtta aagatgaaag gatatacaat caatcataaa acagttagaa aattaatgtc
      781 ccaaatggga cttacctgtc aaatccgaat aaagcgatat aaatcttata aaggaacagt
      841 aggaaaaatt gccaagaatg tgttaaaacg aaatttttca gtagatacac ccaataaaaa
      901 atgggtgaca gacgtcactg aattcaagat aaaaggaaga aaaatctatc tatctcctat
      961 acttgattta tttaacggag aaataattag ttatagtata tcaactagtc caacatataa
     1021 actgattgaa gaaatgcttc aacaagcaat taaaaaaaag ggaactgagg gttcactgat
     1081 tctacattca gatcaaggat ggcagtatca gatgccacaa tatcaaaaaa aattaaaaga
     1141 gaataacatt attcaaagca tgtctagaaa aggaaattgc ttagataatt ctgtaataga
     1201 aaattttttt ggtgtgctta aatcagagtt tttttatcga gaaaaattcc gatcaattga
     1261 gatatttcaa agtaaattaa atgagtatat taggtggtat aataacaaaa ggataaaact
     1321 aaagttaaat ggactatctc cagtagaata tcggaaacag tccattaaat agtt
//
LOCUS       DAJSBL010000167         1314 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.179, whole genome shotgun sequence.
ACCESSION   DAJSBL010000167 DAJSBL010000000
VERSION     DAJSBL010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1314
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.179"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..848
                     /locus_tag="ONT85_002964"
     CDS             <1..848
                     /locus_tag="ONT85_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HCR2379859.1"
                     /translation="EIADLIEKMYGHHYTPQTMSNITKSFTEEVTAFKGRELHDRYAA
                     IYMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEILLDLQER
                     GLKNVLLFITDGLKGMVGAISRFYPKARFQHCCVHVFRNISHKVRVNNRKEVCDDFKM
                     VYQASSKEVALEARGAFAKKWKTSYPKVVESILSNDHLLTFYDFPLAIRKSIYSTNLI
                     ESFNKQIKKYSHRKEQFQNEESMERFLVSSFDTYNQKFLGRSHKGFQQAEGELEQMLS
                     QPMEN"
BASE COUNT          429 a          240 c          254 g          391 t
ORIGIN      
        1 ctgaaattgc tgatttgatc gaaaaaatgt acggtcatca ctatactcca caaaccatgt
       61 ccaatataac aaaatcattt acagaagagg taacggcgtt taaagggcga gagcttcatg
      121 accgttatgc tgctatttat atggacgcaa cgtatattcc gttaaagcgg aaaaccgtcg
      181 ccaaggaagc tattcatatc gcagttggca ttcgcccgga cggatcaaag gaagtattga
      241 gctatgcgat tgcaccgact gaatccatca cgatttggga ggaaatttta ttagaccttc
      301 aagagcgcgg tttgaaaaat gtcctcctgt tcatcacgga tggcttaaag gggatggtag
      361 gagcgattag tcggttctat cccaaagctc gttttcaaca ttgttgtgta cacgtttttc
      421 gtaatatcag tcacaaagtg cgtgtcaata atcgtaagga agtctgtgat gattttaaaa
      481 tggtgtatca agcctcatct aaagaggtgg cgttagaagc acgtggtgct tttgcgaaaa
      541 aatggaaaac cagctatcca aaagtggttg aatcgattct ttcgaacgat cacttgctca
      601 ctttctatga tttccccttg gccatacgca agagtattta ttctacgaac ttgattgaat
      661 cctttaataa gcaaatcaag aaatacagcc accgcaagga acagttccaa aacgaagagt
      721 cgatggaacg tttcttagtc tcgtcttttg atacttacaa ccaaaaattc ctaggtcgca
      781 gtcataaagg tttccaacag gccgaaggcg agctcgaaca aatgctgagt caaccgatgg
      841 agaattagaa acaatctaca gtagggaaga accatttaca caaaattatt gacgctccca
      901 agctaaaact cctattaatc gatctactaa aaataaataa aaaaattctt ttcaattttt
      961 atttatggta tgacataaat aaagtaaagt tattcttcta ggttaatctt attaatactc
     1021 tttctcacta gtgttgcatg caacactagt gagaaagagt attaaatata ctgcaaggca
     1081 agacatttgg taattgaaga ataacaagca aaaaaatagc agtcaggtct cttgacaaag
     1141 tatagttgct attctttttt taggccctac tttaatgaaa tttttgtttg gagggaacga
     1201 cttcattcct ttgattattt cagttgaatt taatagagaa gcctggcgtc tattaagaaa
     1261 tacttttctt gatgagcgtc aattttttta acggaaatct atcctatact aaat
//
LOCUS       DAJSBL010000168         1271 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.180, whole genome shotgun sequence.
ACCESSION   DAJSBL010000168 DAJSBL010000000
VERSION     DAJSBL010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1271
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.180"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            265..957
                     /locus_tag="ONT85_002965"
     CDS             265..957
                     /locus_tag="ONT85_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HCR2379860.1"
                     /translation="MRKKIIINAGVIIGLMLVIFSGKQYADNILLWGGEDNIEKINNN
                     LTILDTALENKEQKISILNSQLSSNNQTLEQLRTNIEVSKQKVASLENDKNQLVSEKT
                     NLENQLSAKDSELQGKQNEINAKIDEINQKVAEINRLNSDWNNRLQNEKNKFNEEQST
                     INSLNQQINQMLSEKNNIQNQLQATQQKLEEEKSKNSSLNNYIEKLQKAKNDVEDTAN
                     KAQQIVNEHTIQ"
BASE COUNT          554 a          144 c          183 g          390 t
ORIGIN      
        1 aattattgac acaacctatt ctggaaactt acaaataaaa gcttattctt cagccacctt
       61 cgggtggtta ttttttataa aaaaatgtaa aaaaatattg atacataaat aaattagttg
      121 tagtaaatag ttttagaatt gaatttctct atccttttta aataattttt tagacaaaaa
      181 acagaaagtg tctaaagaaa gcttgacgaa gaaaataatt tttaataact tatttaagga
      241 aattttttga aggagaagat aaaagtgaga aaaaagatta ttattaatgc tggagtaatt
      301 ataggattga tgttagtaat tttctccgga aagcaatatg ctgataatat tttattatgg
      361 ggaggggaag ataatattga aaagatcaac aacaatttaa ctatattaga cacagctcta
      421 gaaaataaag agcaaaaaat tagtatttta aattcacaat tgtcttcaaa caaccaaact
      481 cttgaacaac taagaactaa tattgaagtt tccaaacaaa aagtggcatc tttagaaaac
      541 gacaagaatc aattagtatc agagaaaact aatttagaaa atcaattgtc agcaaaagac
      601 tctgaattgc aaggaaaaca aaatgaaatt aatgcgaaaa tagacgaaat taaccaaaaa
      661 gtagctgaaa taaatcgatt aaatagcgat tggaataatc gtttacaaaa tgaaaagaac
      721 aaatttaatg aagaacaatc tactattaat agtttaaacc agcaaattaa tcaaatgttg
      781 agtgaaaaaa ataacattca aaatcaactg caagctactc agcaaaagct agaagaagaa
      841 aagtcaaaaa atagtagttt gaataattac attgaaaagc ttcaaaaagc taaaaatgat
      901 gtagaggata ccgcaaataa agcacaacaa attgtaaatg aacatacaat ccaatagata
      961 tagttttaat ttaacaaatg gtatgtttgg agataatgag atagaatata aatgtcataa
     1021 tattatacaa tctaaacacc gtttcctttt ctatttttga tgacatcagt atatagctac
     1081 tcgaaagagt agtcttttta ttattctcgc ttattattga tatgaaaatt tcgatttgac
     1141 aaaaggtacc gaataatttt gtaatatgaa ttattatcaa gaagtaatta tgatgataac
     1201 atatggaaac tagttagtaa taataacagg tgattaaact tttgaacgac ttgtaattct
     1261 atattttttc t
//
LOCUS       DAJSBL010000169         1263 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.181, whole genome shotgun sequence.
ACCESSION   DAJSBL010000169 DAJSBL010000000
VERSION     DAJSBL010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1263
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.181"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..224
                     /locus_tag="ONT85_002966"
     CDS             <1..224
                     /locus_tag="ONT85_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HCR2379861.1"
                     /translation="VFEMNASMYSFKPEFLKKSNYIFDGQCGIIKMRDYLILDIDSEE
                     DFKWLEFLFPKFLEDSSELQDIYNYLYRR"
     gene            229..1176
                     /locus_tag="ONT85_002967"
     CDS             229..1176
                     /locus_tag="ONT85_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HCR2379862.1"
                     /translation="MSCYLITGGAGFIGSTLANHLGEEHVVIVVDDLSMGKVENLDMT
                     KNITFIEGDVAEKSLMEEIMKANKFDYIFHLAAVASVADSVARPVETHRVNFESVLMI
                     LELIRKYQPDLKRIVFSSSAAVYGDEPTLPKKEESVIRPLTPYAVDKFAAEQYVLDYC
                     HLYDVPGSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQIVGEATSFTLFGDGSQSR
                     DFVYIDDVIQALLLVANEEAALGKQFNVGTGKATTLLELIHAINEILGVELTLEYKEE
                     RSGDIHDSLADITKIQSIGYQPKYGILSGMTNYLKTEIN"
BASE COUNT          414 a          179 c          263 g          407 t
ORIGIN      
        1 aagtttttga aatgaatgcc tcaatgtatt cttttaaacc tgaatttcta aaaaaaagta
       61 attatatttt tgatgggcaa tgtgggataa taaaaatgag agattatcta attttagata
      121 ttgatagtga agaagatttt aaatggttag aatttttgtt tcctaaattt ttagaagatt
      181 cttcggagct tcaagatatc tataattatt tatatcggag gtaaaaggat gagctgttat
      241 cttatcaccg gtggggctgg cttcattggt tctaccttag ccaatcattt aggagaagaa
      301 catgtggtaa tcgtcgtcga tgacttatca atgggaaaag tagaaaatct tgatatgacg
      361 aaaaatatca cttttattga gggagatgtc gctgagaaat ccttgatgga agagatcatg
      421 aaagctaata aatttgatta tatctttcat ttagcagcag ttgctagtgt ggcagattca
      481 gttgcccgac cagtagaaac acatcgtgtg aattttgaaa gtgtattaat gattttagaa
      541 ttgatccgca agtatcaacc tgatttaaaa cggatcgtgt ttagttcttc agcggctgtt
      601 tatggggatg aaccgacttt accaaaaaaa gaagagtccg tgattcgccc attgacgcct
      661 tatgcggttg ataaattcgc tgcagaacaa tatgtgctag attattgtca tttatatgat
      721 gtacccggta gcgcagttcg ttttttcaat gtctatggtc caaatcaaaa tccgaattct
      781 ccatacagtg gtgtgatttc tattctcgta gatcgctata agaaacaaat cgtaggagaa
      841 gcaacttcat ttacattatt tggtgatgga agccagtcta gagactttgt ttatatagat
      901 gatgtaatcc aagcattact tttagttgcg aatgaggaag ctgcactggg gaaacaattc
      961 aatgttggaa cgggtaaagc aaccacactt ttggaattga ttcatgcaat caatgaaatt
     1021 ctcggtgtag aattgacact tgaatacaaa gaagagcgtt cgggtgatat ccatgactct
     1081 ctagcagata ttactaagat ccagtcaatt ggctatcaac cgaaatatgg tattttaagt
     1141 ggaatgacta attatttgaa aactgaaatc aattaaaaca tactaagata agattagagg
     1201 gtaaattcag gctctttgtc aataaggact gatgagtttt gtaaaatcaa atctgggata
     1261 gaa
//
LOCUS       DAJSBL010000170         1233 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.182, whole genome shotgun sequence.
ACCESSION   DAJSBL010000170 DAJSBL010000000
VERSION     DAJSBL010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1233
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.182"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            358..444
                     /locus_tag="ONT85_002968"
     CDS             358..444
                     /locus_tag="ONT85_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HCR2379863.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            complement(565..>1233)
                     /locus_tag="ONT85_002969"
     CDS             complement(565..>1233)
                     /locus_tag="ONT85_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2379864.1"
                     /translation="LLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLPGVIFTLS
                     IVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTTCYEEMND
                     IFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQTEEACYNL
                     FSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKSVKEWLDQ
                     QQQN"
BASE COUNT          348 a          244 c          205 g          436 t
ORIGIN      
        1 taaacaggtt cgcgcatttt tcaaaactgc caatccaaaa ccttgcttcc tacggtcgcc
       61 aaaaaggtgt gatcgctacg ctctcacatt gtactgcgca gtcgcctaaa ggctcctttg
      121 ctaaaaacat gttggtaaag aggcctttga gcggttctga atgcttatct aagccgtttt
      181 aagacagtct tggataaaaa tccttatcta gctttaaagc gccttacagg cggtcagggc
      241 aagccctaac aaccctaaaa taagcgatac ttaaaaacac cacacaagtt taatttttta
      301 agatataata acttgtcgcc tatccgacag tcttgcgact ggaaggaggt gtatctaatg
      361 tttgaattgt tcatttgccc ccttcttata gaagtttgtg tctctttatt cagttattgg
      421 ttgaataacc gaaacaagaa gtaaaatcaa taaaggcgac atcaacctga aaaaaagccc
      481 cagagaatcc gacctctctg gggctttcgt gtatctaatg tttgatcatt tgcatgttta
      541 gtttaacatg attgccgatc aaattcaatt ttgctgttgc tgatctaacc attccttgac
      601 actttttaga taatttaatg ccgtttcttt tttcgttgga ttttgtatgt tcgttgcttg
      661 ctgaagcagt gctttataac gttcgtaggt aactgattgt tgccgttctt ttcgttcctg
      721 ttcactagtt gaaaaaggga ttgtatcact gaataaatta taacaagctt cttctgtctg
      781 cgtatcttta aaatattgca cgtctttttt ggttcgctca tagttgtctt gaacctcttt
      841 ttgctgtttt tctggcaagt tttgataaag acgagtgagt ttttccatgt ttttatccgt
      901 caaacgtttt tcttgattca caaaaatatc gttcatttcc tcataacaag tggtaaactc
      961 ttccattttt tgtgttaaca cttgctcctt tttcttttta tcacatgctg cttcatactt
     1021 ttgagtgata tcccctaata aaatacttct tgaaatacgt ttatttccct ctaaaacgcc
     1081 gaataaacca cttaaaacga ttgataatgt aaatataacc cctggcaata aaaacctctt
     1141 aaatcgaatt ttagagcctt ttaatgaata tcttgtttga taagccttat agctaagcca
     1201 aataattcct aaaatactgg caaaaatgag tag
//
LOCUS       DAJSBL010000171         1193 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.183, whole genome shotgun sequence.
ACCESSION   DAJSBL010000171 DAJSBL010000000
VERSION     DAJSBL010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1193
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.183"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..1076
                     /locus_tag="ONT85_002970"
     CDS             <1..1076
                     /locus_tag="ONT85_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HCR2379865.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGKG
                     RFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQV
                     IVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQS
                     QNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARKF
                     TRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT          418 a          234 c          210 g          331 t
ORIGIN      
        1 ttgtgattgg catggagtca acgtccatgt acagcttcca tccttccatg ttttttcatg
       61 aagatgagaa actaaagaag ctcaacacac ttgtaacgat tgaaaatcct tttcgggtga
      121 aacaatttag tcgcatgttt gatgagaata aaactgatcg aaacgatgca ttaagaatcg
      181 ctgatttctt gcgtattcaa cgattcacaa cttcccctat caaagaagag aaatacatgg
      241 ccttacagcg tttaacacgt actcgctatc aacttattaa gcaattgatt cgtacaaagc
      301 aacattttct tgaaaatcta acgtacaaat gtaatacgct tgctcgagaa atgcgagacg
      361 aatcgacgag tctttttagt gcaaccctca tttcactcat gacggaagat ttcacattgg
      421 atgaacttgc tgagcttcct ttagaagctt tctgtgattt acttcaagaa aaaggaaaag
      481 gccgtttcaa gcaaccagaa aaaatcgcta aagcaattca acgtgcaatt accatgagtt
      541 accgcctagg tgctcttgca caagaatcaa ttaatgttgt tttaagtgtt ctcgtacgag
      601 aaatacgagc acttgaaaaa aatatcaaag aattagataa agccatcgaa caagtaattg
      661 ttgtcattcc agaatatcaa tgcttaacca gtattccagg cgttggtaaa gtctatgctg
      721 ccggtttaat cgctgaaatt ggtcaaatcg aaagatttga agatcagaca aaattggcaa
      781 aatacgctgg attaagttgg aaagtgaacc aatctggaaa ctatcagtcg caaaatacac
      841 ctctgacaaa acaaggaaat cgatatttta gatactactt agttgaagct gccaactctg
      901 taaaaaatta tcttcctgaa tacaaagctt tttatcaaag taaatataag gaggttccaa
      961 agcatcaaca caaacgtgca ctcgtcctaa ccgcaagaaa atttacgcgt ctggtggata
     1021 cgctactacg taaacaccaa ctctatacgc caccaaggag tgtgatagag aaataacaat
     1081 ccgttattga cgcaaatcct tgaatcaacc cgaaaaaaat ttgattttga tcgggtctag
     1141 tttcgtgcgc ttcaaaacat attactcaat aaagaaaatc ttaaatttca tct
//
LOCUS       DAJSBL010000172         1176 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.184, whole genome shotgun sequence.
ACCESSION   DAJSBL010000172 DAJSBL010000000
VERSION     DAJSBL010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1176
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.184"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..>1176
                     /locus_tag="ONT85_002971"
     CDS             <1..>1176
                     /locus_tag="ONT85_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HCR2379866.1"
                     /translation="ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCL
                     THYQHTYCCKSCEKEGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQE
                     NYWKQTHGLDISRDNITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESA
                     KTDTYYWQFCTGKDSQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESV
                     RLVYCFAHVRRKFFEAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQ
                     QELRPLFIAFYEWMATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERN
                     IKELVIGRKNWLHSTSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVL"
BASE COUNT          385 a          208 c          257 g          326 t
ORIGIN      
        1 ctataacaga gaaagaacat ctccttcctt tggaagaaca attttgtgaa cattgcggtt
       61 cacagatgaa agatatcgga cggacaaaag tacgagaaga aattcgtttc catcaggcta
      121 tgttggattg tctcacccat tatcaacata cctattgttg taaaagctgc gaaaaagaag
      181 gactttcttc ttttaaaaaa gcgatcgttc ctaaaccttt gatttctaat agcttaggtt
      241 ccaatagttt agtggcggaa acgatccgga tgaaatttgg tcagaaagtt cctgcctatc
      301 ggcaagagaa ctattggaaa caaacacatg ggttggatat ttcgagagat aatatcacca
      361 attggcatat taaagcagtg caaaacgcgt tagacccttt aggagaacga ttaagagtct
      421 atcttaatca agaagagatt ctacacgggg atgaaacaag ttatcgtgtg attgaaagtg
      481 ccaaaacaga tacctattac tggcagtttt gtacaggaaa agacagtcag catcccatcg
      541 tttattatca ccacgacgaa agtcgtgctg gagatgttcc gaaaactttt ctcaaggagt
      601 ttacagggta tttacactgt gacggctata gcgggtataa tgccgtggaa agtgtacgac
      661 ttgtctattg tttcgcgcat gttcggcgga aatttttcga agcaattcca aaaggaaaga
      721 aaaacacgga tattcccgca gctcaagcag tcaaacaatt ggataaatgg tttgtcctag
      781 aaaagaaatg gaaagacttt tctcctgaga agcgattaag ctgtcggcaa caagaactgc
      841 gtccattatt tattgctttt tatgagtgga tggcaacgat agatcctgtt gcaaaatcca
      901 agttggatgc agccgttcaa tatgcgtgta aactacgaag tggttttgaa cctatttttg
      961 aagatggtcg tttagaactc acaaataatc gtgcggagcg aaatatcaaa gagctggtga
     1021 taggacggaa aaattggttg cattccacaa gtcttgaagg agcacgaact tcaggaatca
     1081 ttctgagtgt atataaaaca gcagaactta acggattgaa tcctgtaaag tatctagagt
     1141 ttctatttga taagatacca aatcttcccg tacttt
//
LOCUS       DAJSBL010000173         1143 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.185, whole genome shotgun sequence.
ACCESSION   DAJSBL010000173 DAJSBL010000000
VERSION     DAJSBL010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1143
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.185"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(18..923)
                     /gene="xerC"
                     /locus_tag="ONT85_002972"
     CDS             complement(18..923)
                     /gene="xerC"
                     /locus_tag="ONT85_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HCR2379867.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
BASE COUNT          338 a          232 c          194 g          379 t
ORIGIN      
        1 tgttcttttc cttattttta cgctcttgga tggaaagaac gatagttttt ttgtaaactt
       61 tcttttgtta catgggcata gatctgggta gtcgataaat tggcatgtcc tagcaattct
      121 tggactgtcc gcaaatctgc cccattattt aataaatgag tagcaaatgt atggcgcagc
      181 atgtgaggat ggatatcact atccaaactg ctttttttga tcatttgatt caagacatat
      241 tcaattccag taggcgtgat tggttttcca tgatgattca caaaaacgat atcatgctct
      301 tcgtgatatt ttgtcatcaa tacctggcgg ccttccctga aatattcttg cagagcatct
      361 tgagcaaaag aacctagagg agcatatctc tctttgttcc ctttaccatg gatcaacatg
      421 acctctgaat caaaatcaat gtctgatacc gacaaactgc tacactcact taaacggatg
      481 cctgacccgt aaaggacttc cagtaaagcg cggttcctca aatccaaagg actatctcct
      541 gctacgctgt caaaaagtgc ttgcatttca ttttcataaa aaaaacgtgg cagtctcaaa
      601 tttttctttt tgagatgaac gtaagaaaat ggattttctt ttatcacttc ttctttgaga
      661 agatactgat aaaacgaacg taggctggct atcttgcgac tgatagaatt tctgctatag
      721 ctaggacgat gatcagctaa tgcactcaaa tagacacgaa ccgtaaaata ttccgccttc
      781 agcagatcag cgtctccaga ttcctccaaa aaatgtttga aatggagaat gtcttcttca
      841 tatgcttctt tcgttttttc tgaatatcct ctttcgacaa tcaaataacg caaaaatttt
      901 tgttcccacg tggcttctgt cattttctca ccttcctttt gtcccagaga ataatttagc
      961 acagcgttta taaaaagaca aataaattgt cagaatttaa ccaatatttc taagtttgtt
     1021 cacaattggc gcaattcctc acattttgtt tgagaaattc ttaagaaaaa cgcaagaaaa
     1081 agggctctat aatttatggg actgatgaat cagaattgat tcttcagccc cattttcatt
     1141 tta
//
LOCUS       DAJSBL010000174         1064 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.186, whole genome shotgun sequence.
ACCESSION   DAJSBL010000174 DAJSBL010000000
VERSION     DAJSBL010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1064
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.186"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(39..995)
                     /locus_tag="ONT85_002973"
     CDS             complement(39..995)
                     /locus_tag="ONT85_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2379868.1"
                     /translation="MELYTLPIIIKTEKMLKDLKQYYPLSAYEKKKLFHFLAINLLAE
                     QADSLDIASNQSLFFSDIGFYHLKGVLGEKSVIFINKTLSWLYLHDKLDQKYLRKNED
                     ETIEWLNERFIESFENQFNLLREETSQLIRRELTKIHFKLLHYTINKADEFPLDVSFF
                     RENYPAFYCYLIEYINDLTSKRKELLQDKFFLFFSYLLLLINHIPIQLISEPVKIIID
                     FSYGAAYNQFIKKNLSLYINLNTEVIEAGKPEFPDVIITNRNNLYEESASQVVVWLDP
                     PRAVDWGNLTKILLTIQEEKYKDSKIDKQIDDFPEEIIDRIE"
BASE COUNT          346 a          177 c          160 g          381 t
ORIGIN      
        1 ttttagaaaa agcagtcgga ctttctaaaa gaagttcact attcgatcct gtcgatgatt
       61 tcttcaggaa aatcgtctat ttgcttgtca attttgctgt ctttgtattt ctcttcttgg
      121 atagtcaaca aaatctttgt aagattcccc cagtctactg ctcttggcgg atcgagccaa
      181 accactactt gagatgcact ttcttcatat agattattcc tattcgtgat tataacatcc
      241 gggaactcag gctttcctgc ctctatcact tccgtgttca gattaatgta caagcttaaa
      301 tttttcttga taaattgatt ataggcagca ccataagaaa aatcgatgat gatctttact
      361 ggttcagaaa tcagttgaat gggtatgtga ttgatcaata ataataaata actgaaaaaa
      421 agaaaaaatt tatcttgaag caactctttg cgttttgacg ttagatcatt tatatactct
      481 atcaaatagc aataaaatgc tggatagttt tctctaaaaa aagaaacatc aagtggaaat
      541 tcatcagctt tattaatagt gtaatgcaaa agtttaaaat gtattttggt taactctctt
      601 cggatcaatt ggctcgtctc ttctcgcaac agattaaact gattttcaaa actttcaatg
      661 aatcgttcat tcaaccattc gattgtttca tcttcatttt ttctcaagta tttttgatcc
      721 aatttatcat gaagataaag ccaagagagt gtcttattga taaaaataac ggatttttct
      781 cctaatactc cttttaagtg gtaaaatcct atatccgaaa aaaatagcga ctgattgcta
      841 gcaatatcta aagaatcagc ttgttcagcc aacaggttga ttgctaaaaa gtggaataat
      901 ttcttttttt cataagcaga cagaggataa tactgtttta aatcttttaa cattttttct
      961 gtttttataa ttataggtag cgtatacaat tccacaaaac ttatctctgt tgagttaaaa
     1021 agattatata aaaaaagccg aatgttttta ggattccctg tcaa
//
LOCUS       DAJSBL010000175         1051 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.187, whole genome shotgun sequence.
ACCESSION   DAJSBL010000175 DAJSBL010000000
VERSION     DAJSBL010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1051
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.187"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            6..980
                     /gene="bsh"
                     /locus_tag="ONT85_002974"
     CDS             6..980
                     /gene="bsh"
                     /locus_tag="ONT85_002974"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HCR2379869.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTVTPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGEAKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAIDMNKEDLDSHKLISYPIIEKQQIKYIN"
BASE COUNT          377 a          132 c          182 g          360 t
ORIGIN      
        1 aaactatgtg tacgtctatt acttatgtaa caagtgatca ttattttgga aggaattttg
       61 attatgaaat atcttacaat gaagtagtta ctgttactcc aagaaattat aagttgaatt
      121 ttcgaaaggt aaatgatttg gatactcatt atgcaatgat tggtattgcc gctggtatag
      181 ctgactaccc tctttattac gatgcgacaa atgaaaaagg attgagtatg gctgggctaa
      241 atttttctgg gtatgctgat tataaagaaa tacaagaagg gaaagacaat gtatctcctt
      301 ttgaatttat tccttggatt ttaggacaat gctcaacagt aggagaagct aaaaaattgt
      361 taaaaaatat caatttagca aatataaatt atagtgacga acttccttta tcccctttac
      421 attggctatt agctgataaa gaaaaatcaa ttgtcattga aagtatgaaa gatggacttc
      481 atatatatga taaccctgtg ggcgttctta ccaataatcc ttcatttgac tatcaattat
      541 ttaatttaaa caattatcgt gtcttatcga gtgaaactcc taaaaataat ttttcaaatc
      601 aaataagttt gaatgcctat agccgcggta tgggagggat aggcttgcct ggagatttat
      661 cctcagtatc tcgttttgtt aaagcgactt ttacgaagct gaattctgta tctggagatt
      721 cagagtcaga aagtattagt caatttttcc atatcttagg ttcagtagaa caacaaaaag
      781 gtttgtgtga tgtaggtgat ggaaaatatg aatatacaat ttattcttct tgttgcaatg
      841 ttgacaaagg aatctattat tatcgaacat atgaagacag tcaaattact gcaattgata
      901 tgaataaaga agacttagat agtcataagt taattagtta tccaattata gaaaaacaac
      961 aaattaaata tataaattag ttaatgtgtt gtgattgatt atttaatata gatataatac
     1021 aaaaaggcaa agatattttc aaaacatttt t
//
LOCUS       DAJSBL010000176         1043 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.188, whole genome shotgun sequence.
ACCESSION   DAJSBL010000176 DAJSBL010000000
VERSION     DAJSBL010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1043
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.188"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..704
                     /locus_tag="ONT85_002975"
     CDS             <1..704
                     /locus_tag="ONT85_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HCR2379870.1"
                     /translation="YSVIFMDATHIPVKRQTVSKEAVYITIGIRLDGTKEVLGFTIAP
                     TESAYIWKEVLQDLRKRGLEEVLLVVTDGLSCIEESIHSVYPNAQFQQCCVHVSRNIA
                     HKVRVRGRKEICEDFKLVYQANSKEEALDHIDFMTRKWKKQYPRVVNLLLNPALLTFY
                     NFPHAIRRTIYSTNLIEGFNKQLKRYTRRKEQFPNEESLERFFISQFNQYNQKFLGRI
                     HKGFKEIQDTLESMI"
BASE COUNT          354 a          168 c          173 g          348 t
ORIGIN      
        1 attattccgt tatatttatg gatgcgacgc atatccctgt gaagcgacag actgtttcga
       61 aggaagcagt gtatattacg attggtattc gtttggatgg aaccaaagaa gttcttgggt
      121 ttactattgc cccaactgag tctgcttata tttggaaaga agttcttcaa gatcttagaa
      181 aacgtgggtt agaagaagtt ttattagtag tgacagacgg attaagctgt attgaagaaa
      241 gtatccatag tgtgtatccg aatgcccaat ttcaacaatg ttgtgtgcat gtatctagaa
      301 atatcgctca taaagttcgt gttcgaggtc gaaaagaaat ttgtgaggat ttcaaattgg
      361 tttaccaagc gaattcaaaa gaagaggcat tggatcacat cgactttatg actaggaaat
      421 ggaaaaagca gtatccaaga gtcgtcaatt tactcttgaa tcctgcccta ttaacctttt
      481 ataatttccc tcacgccatc agacgaacaa tttattcgac gaacctgatt gaaggcttta
      541 ataagcagct aaaacgatat actcgaagaa aagaacaatt ccctaatgaa gaatctctag
      601 agagattctt catttctcaa tttaatcaat ataaccaaaa atttttaggt agaattcaca
      661 agggatttaa agaaattcag gatacattag agtcgatgat ttaactgtaa ttaacagaat
      721 ggattttcca tttacatata attcttgacg ctaccataat ctaaataacc atcatattca
      781 tctactactt atataattga gttttattaa actttttaag tttctattct atcaaatatt
      841 taaacaatct ctgcatccga aaagactacc tcgtatagtt ttactaacaa tatttttatt
      901 agcgtgttca aatcctgatt tcttgtatca attattttct gttcactttc aataagaaaa
      961 aagtctttac ttccatggat aataataagg gttctgttgc aaagttttaa ataaagaata
     1021 aaatccctta cggtatctat gat
//
LOCUS       DAJSBL010000177         1035 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.189, whole genome shotgun sequence.
ACCESSION   DAJSBL010000177 DAJSBL010000000
VERSION     DAJSBL010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1035
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.189"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            402..1035
                     /locus_tag="ONT85_002976"
                     /pseudo
     CDS             402..1035
                     /locus_tag="ONT85_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          357 a          183 c          186 g          309 t
ORIGIN      
        1 taataaaata ttttttatat tacaaaagta gtgtttcaaa gttagtagag aaaatgtagt
       61 tatatattta aatttttacc agaaagaaat gaacaccttc aattatctaa atctcgaaaa
      121 tggttagatg taatcatcag gaaaaacaat cttaaacgaa ttactattca tggtcttaga
      181 cacacacatg ctagcttgct tttagcaagc tagtgcaaat attaaagatg tgcaagagcg
      241 tttaggccac tcttctattc aaatcattat ggatctatat attcatatta cagacaaaag
      301 aaaagaagaa accgtagcgc aattcgcaaa atataggctc tataatttat gggactgatg
      361 aatcaaaatt gattcttcag tcccattgtt attttaggta tatgattcgt acattgaaag
      421 agttgaaaaa ctatcttccg aagccgtcaa aaaggaccct gccaaaagta ttgatggtgg
      481 atgaatttcg ttcgcacgct tcaattgtgg ataaaatgag ctttatttgt gcggatggcg
      541 agactggaaa actcattgat attttgccta cacgaaaatt gtctcgatta acacactatt
      601 ttagtcgctg tgcaaatcct gaagcggtgg aatatttagt gacagatatg aacgccgcct
      661 acttccaact gacaaaacgt gtcttaccaa atgcgaaagt cgtcattgat tgttttcata
      721 tcatcaaaca tttgaatcag gcgttcaatg attttcgtgt acaagaaatg aaaagattac
      781 accagttagg gcaacgacgg gaagccgaga aagtgaaaaa gaactggcgc tttcttctaa
      841 aaaaccgtca aaaaatcaat cactatgatt acaaaacatg gcgcagtttt cgtgcaccta
      901 aatacccgct actcactgaa gcaatgatga ttgatcgatt gctggcattt tcaacgcctt
      961 taaaggacgc ctatactttt ttccatgagc tgaccgaagc ctttagagac aaggaatcag
     1021 acttattttt tacct
//
LOCUS       DAJSBL010000178         1004 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.190, whole genome shotgun sequence.
ACCESSION   DAJSBL010000178 DAJSBL010000000
VERSION     DAJSBL010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1004
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.190"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..>1004
                     /locus_tag="ONT85_002977"
     CDS             <1..>1004
                     /locus_tag="ONT85_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HCR2379871.1"
                     /translation="LKKQRFKCKSCQRTFVADTSVAEKHCFISQKVRWSVIARLKENT
                     SMTEIARQKNISTSSVYRVMKRFYRPLNPFKQTLPKVLCFDEFKSVRSVASAMSFIMM
                     DGQSHALLDIVENRRLPYLERYFSRFSLATREAVQLIVIDMYAPYVSLVKKLFPNAQL
                     IIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHSQRGLGKKLKRYWKLLQKEEAKLNYE
                     KRVWRASFKDYLTESEIADRLLAACPNLRQGYQLYQDILYAVKKRDQSLFEDCLTRDI
                     SGLPETYTTTLRTFKKFLPQIQNALHYSYSNGPLECLNNHIKVLKRNAYGFRNFYNFK
                     "
BASE COUNT          313 a          203 c          195 g          293 t
ORIGIN      
        1 agctgaagaa acaacgattt aaatgtaagt cttgccaaag aacattcgta gcggatacct
       61 ctgttgctga aaaacactgt ttcattagtc aaaaagttcg ttggtctgtc attgctcgct
      121 taaaagagaa tacttccatg acggaaatcg ctcgtcagaa aaatatttcc acttcttcgg
      181 tctatcgtgt catgaagcga ttctaccgcc cattgaatcc tttcaaacag actttgccaa
      241 aggtcttatg ctttgatgaa ttcaaatcgg ttcgaagtgt tgccagtgca atgagtttta
      301 tcatgatgga cggacaaagc catgcattat tggatattgt cgaaaatcga cgcttgcctt
      361 atttagaacg ctatttttct cggttttcat tagcgactcg tgaagccgtt caattgattg
      421 ttattgatat gtatgcgcct tatgtttcgt tggtgaaaaa gcttttcccg aatgctcagt
      481 tgatcatcga tcgtttccac atcgtccaac atattggcag aactttcttg aatcatcgag
      541 tgaaagaaac cacggtacga cttaaaggaa attcccactc tcaacgaggg ttagggaaaa
      601 aattgaagcg ctattggaaa ctcctacaaa aagaagaagc aaagttaaac tatgaaaaac
      661 gggtttggcg tgccagtttt aaagattatt tgactgaaag tgagattgcc gatcgactgt
      721 tagctgcttg tcccaactta cgccaaggct atcagttata ccaagacatt ctttatgcgg
      781 taaagaaaag agatcagtca ctatttgagg attgtttaac tagagacatt agcggtctgc
      841 cagaaaccta tacaaccact ttacgaacgt ttaaaaagtt tctgccacag atccaaaatg
      901 cgcttcacta cagctattca aacggaccat tggaatgctt aaacaaccac atcaaagtat
      961 taaaacgcaa tgcgtacggc tttcgcaact tttacaattt caaa
//
LOCUS       DAJSBL010000179          996 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.191, whole genome shotgun sequence.
ACCESSION   DAJSBL010000179 DAJSBL010000000
VERSION     DAJSBL010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..996
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.191"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(25..912)
                     /locus_tag="ONT85_002978"
     CDS             complement(25..912)
                     /locus_tag="ONT85_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HCR2379872.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
BASE COUNT          288 a          207 c          179 g          322 t
ORIGIN      
        1 aatttctttg cctttttatc taaatcaaac gtttagttct tgaccaacta ataaagaata
       61 actggacata gacatgccat ttttcgccat tagatcatca acagacatgc caaatttttg
      121 tgcaatatcc catagcgtat cacctgacac gactgtatat gtctgaccat tatttgtatt
      181 tacatctgtt gttgaagatg atgacgttga agtcgtcgta gtagtcgttg ttgttgtact
      241 tgtggttgtt gtagccgaac ctgttgatgg cgtatcaaaa cgggttaaat tataagtatt
      301 aatcaatgag ttcaatttgg ccccataatt tggatcggta gcataacgac ctgtcaagta
      361 cgctgttgca tcataaaaac ttgttgtatt gcttttccac acgccagcat aatgataatc
      421 gcccgtggaa aaactggttg aacgaagaac attcgcatgg tcttggaagg attcccaata
      481 agaagaatat tttcggaatg gctcattcat cgttacccat tgtccgttta catattcttt
      541 ggttgccata taaacgacct gtccgttata gcttcctttc acaccgaata gattataatt
      601 tggcgcacta gctaatccgg aagtgccata tgcactttcc aacaacgctt gagcgatcat
      661 gactgaagca tacaagtcat tacttgcagc aacttctgac gcagaccagc cgatttgatc
      721 gataaacgct tgaggatcag cgactgtttg agatccttgt tcatctgcat gaacatgttg
      781 cggtaccgct gtaaccatca tcggcccaac taaaagactc gttccaatta aaggagcaat
      841 cgctttttta ttgatcacca tttttctttt aggtctatgg tgccgggcac tgcgggtctt
      901 cagctcttgc attcaaattc ctcctcaaaa taaaaaaact taataaaaag ttctcttctt
      961 aaaacaaact taattaatta aattatactt ttaatt
//
LOCUS       DAJSBL010000180          994 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.192, whole genome shotgun sequence.
ACCESSION   DAJSBL010000180 DAJSBL010000000
VERSION     DAJSBL010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..994
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.192"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..>994)
                     /locus_tag="ONT85_002979"
     rRNA            complement(<1..>994)
                     /locus_tag="ONT85_002979"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          209 a          307 c          225 g          253 t
ORIGIN      
        1 agcacgtgtg tagcccaggt cataaggggc atgatgattt gacgtcatcc ccaccttcct
       61 ccggtttgtc accggcagtc ttgctagagt gcccaactga atgatggcaa ctaacaataa
      121 gggttgcgct cgttgcggga cttaacccaa catctcacga cacgagctga cgacaaccat
      181 gcaccacctg tcactttgcc cccgaagggg aagctctatc tctagagtgg tcaaaggatg
      241 tcaagacctg gtaaggttct tcgcgttgct tcgaattaaa ccacatgctc caccgcttgt
      301 gcgggccccc gtcaattcct ttgagtttca accttgcggt cgtactcccc aggcggagtg
      361 cttaatgcgt tagctgcagc actgaagggc ggaaaccctc caacacttag cactcatcgt
      421 ttacggcgtg gactaccagg gtatctaatc ctgtttgctc cccacgcttt cgagcctcag
      481 cgtcagttac agaccagaga gccgccttcg ccactggtgt tcctccatat atctacgcat
      541 ttcaccgcta cacatggaat tccactctcc tcttctgcac tcaagtctcc cagtttccaa
      601 tgaccctccc cggttgagcc gggggctttc acatcagact taagaaaccg cctgcgctcg
      661 ctttacgccc aataaatccg gacaacgctt gccacctacg tattaccgcg gctgctggca
      721 cgtagttagc cgtggctttc tggttagata ccgtcaaggg atgaacagtt actctcatcc
      781 ttgttcttct ctaacaacag agttttacga tccgaaaacc ttcttcactc acgcggcgtt
      841 gctcggtcag actttcgtcc attgccgaag attccctact gctgcctccc gtaggagttt
      901 gggccgtgtc tcagtcccaa tgtggccgat caccctctca ggtcggctat gcatcgtggc
      961 cttggtgagc cgttacctca ccaactagct aatg
//
LOCUS       DAJSBL010000181          974 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.193, whole genome shotgun sequence.
ACCESSION   DAJSBL010000181 DAJSBL010000000
VERSION     DAJSBL010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..974
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.193"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            82..>974
                     /locus_tag="ONT85_002980"
     CDS             82..>974
                     /locus_tag="ONT85_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HCR2379873.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINH"
BASE COUNT          331 a          182 c          191 g          270 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcaccac aaaataaaca
       61 actgagagga cgatacgcta tatgaatgat tctatcaaaa aaatgctgag aataacagag
      121 aaagatttga tgattacaga ggtctcttac gagacccttc agaagaaaaa gacgttggtc
      181 gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa gttgtggttc tactgtggta
      241 gatggaaacg ggaaagcaat tatagtgaaa aatggaaaaa aggaaacgat tgtccgtttt
      301 gaacaataca atcatatgcc tttggttatg cgcctaaaaa agcaacgcta tacctgtaaa
      361 aactgtcgca cccattggac ggctcaaagt tattttgtcc aacccagaca ttcaatcgca
      421 aatcatgtta gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaagct gtcaggtatc tttgaccacc gttattcgta cattgaaaga gtttaaaagc
      541 tatttaccaa agcaatctaa gaagatcctc cccagagtat tgatggttga cgaatttcgt
      601 tcgcatgctt ccatagaaga taagatgagc tttatttgcg cggatggcga aacaggaaaa
      661 ttaatagatg ttttgcctac gcgtaaatta cctcgattaa caagctattt cttaggttgt
      721 accaatccag aagaagtaga attcttggtg acagacatga acgccgccta cttccagctc
      781 accaaacgtg ttctgccaaa tgcgaaagtc gtgattgatc ggtttcatat tgtcaaacac
      841 atgaatcaag cgttcaatga gttgcgtatc cgtgaaatga atgaacttcg taaagccgga
      901 cagaaaagcc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc atta
//
LOCUS       DAJSBL010000182          920 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.194, whole genome shotgun sequence.
ACCESSION   DAJSBL010000182 DAJSBL010000000
VERSION     DAJSBL010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 920)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 920)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..920
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.194"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..>920
                     /locus_tag="ONT85_002981"
     CDS             <1..>920
                     /locus_tag="ONT85_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ISL3-like element ISEfa5 family transposase"
                     /protein_id="HCR2379874.1"
                     /translation="QKKKTLIVDAVFSPAPHTCRNCGSTVVDGNGKVIVVKNGKKETI
                     VRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQSYFVQPRHSIANHVRYKIASLLTEKV
                     SLSFIAKNCQVSLTTVIRTLKEFKSYLPKQSKRILPRVLMVDEFRSHASIEDKMSFIC
                     ADGETGQLIDVLPTRKLPRLTSYFLGCANPEEVKFLVTDMNAAYFQLTKRILPNAKVV
                     IDRFHIIKHMNQAFNELRIREMNELRKVGQKSQAEKLKKNWRFLLKNRANINHYEYKT
                     WKSFRAPKYPFLTEAMMIDRLLGFSTSLKE"
BASE COUNT          300 a          175 c          183 g          262 t
ORIGIN      
        1 ttcagaagaa aaagacgttg atcgtcgatg ctgttttctc acctgctccc catacctgta
       61 gaaattgtgg ttctactgtg gtagatggaa acgggaaagt aattgtagtg aaaaatggga
      121 agaaggaaac gattgtccgc tttgaacaat acaatcacat gcctttggtt atgcgcttaa
      181 aaaagcagcg ctatacctgt aaaaactgtc gaacccattg gacggctcaa agttattttg
      241 ttcaacccag acattcaatc gcgaatcatg tcagatataa aattgcttct ttactgactg
      301 aaaaagtatc tttatctttt attgcgaaaa attgtcaggt atctttgacc accgttattc
      361 gtacgttgaa agagtttaaa agctacttac caaagcaatc taagaggatt ctccccagag
      421 tattgatggt tgatgaattt cgttcgcacg cttccataga agataaaatg agctttattt
      481 gcgcagatgg cgaaacagga caattaattg atgtcttgcc tacgcgtaaa ttgcctcgat
      541 taacaagcta tttcttaggc tgcgctaatc ctgaagaagt aaaattttta gtcacagaca
      601 tgaacgccgc ctacttccag cttaccaaac gtattctgcc aaatgcgaaa gtagtgattg
      661 atcggtttca tattatcaaa cacatgaatc aagcctttaa tgagttgcgt atccgtgaaa
      721 tgaatgaact tcgtaaagta ggacagaaaa gtcaggcaga aaaactgaaa aagaactggc
      781 gctttttgct aaaaaatcgt gcaaacatca accattatga atacaaaaca tggaaaagtt
      841 ttcgagcacc aaaataccca tttcttacgg aagcgatgat gattgatcga ttgcttggct
      901 tttctacgtc cctgaaggag
//
LOCUS       DAJSBL010000183          856 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.195, whole genome shotgun sequence.
ACCESSION   DAJSBL010000183 DAJSBL010000000
VERSION     DAJSBL010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..856
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.195"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            83..>856
                     /locus_tag="ONT85_002982"
     CDS             83..>856
                     /locus_tag="ONT85_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR2379875.1"
                     /translation="MQGVIAMMSKNNTNGRNQFAMLTIDDLVPQDHLVRKIDAALDFE
                     FIYPIVEATYSDLGRPSIDPVILIKLVFIQYLFGIRSMRQTIKEVDTNVAYRWFLGYS
                     FEEKIPHFSTFGKNYVRRFRETTVFEDIFAYILEQAVKAGFVTEDNFHLDSTHIKANA
                     NKHNFTKEMTHDEAKAYQDELEDEINRQRIEAGKRPFTLDLEKEVKLKERKISKADPE
                     SGYYVKGEQEKQFAYSAHTSCDDNGFILSTIITPGNIHDS"
BASE COUNT          285 a          158 c          165 g          248 t
ORIGIN      
        1 ataaaatgcc agactgtaga caaagtccat tttatggatg atgtctacag tcttttcttt
       61 tataatagat ttatgaaaac gaatgcaagg agtgatagct atgatgtcta aaaacaatac
      121 gaatggtaga aatcaatttg cgatgcttac gattgatgat ctagttcctc aagatcatct
      181 cgttcgaaag attgatgccg cgcttgattt tgaatttatt tatccaatcg tcgaagccac
      241 ttactccgat ttaggtcgcc caagtatcga cccagtgatt ctgatcaaat tggtgtttat
      301 tcagtattta ttcggtatcc gttccatgcg acaaaccatt aaggaagtcg ataccaatgt
      361 cgcttaccgc tggtttttag gctactcctt cgaagagaaa atcccccatt tctcaacttt
      421 tggaaagaat tatgttcgcc gatttagaga aacgacggtg tttgaggata tttttgccta
      481 tattttagaa caagcagtta aagcaggttt tgtaacagag gataattttc atctagattc
      541 tacccacatc aaagctaacg ccaataaaca taattttacg aaagaaatga cccatgatga
      601 agcgaaagcc tatcaggatg aattagaaga tgagatcaat cgtcaacgaa tagaggctgg
      661 aaaacgccct tttactttgg acttagaaaa ggaagtgaag ctgaaagaaa gaaaaataag
      721 taaagctgat ccggaaagcg gctactacgt caaaggcgaa caagaaaaac aatttgctta
      781 ttctgcccat acttcatgcg acgacaatgg atttattctt agcacaatca tcactcctgg
      841 aaatattcat gacagt
//
LOCUS       DAJSBL010000184          815 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.196, whole genome shotgun sequence.
ACCESSION   DAJSBL010000184 DAJSBL010000000
VERSION     DAJSBL010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..815
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.196"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            533..>815
                     /locus_tag="ONT85_002983"
     CDS             533..>815
                     /locus_tag="ONT85_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HCR2379876.1"
                     /translation="MSKKGTIINEWEKLTAPSNRRLKYQSLTLPLKMQWSIDRITIVG
                     KLKENIYYHTPNDVLILNFEQLMRLNEGNGYLRSVSNNGWQLLDQHEENI"
BASE COUNT          270 a          130 c          172 g          243 t
ORIGIN      
        1 ttagagcagg agaaaacctt attttatcgt caaaacgtct cacagcgtaa attagagcgt
       61 tctgaagatg agatcgagtg gtagttacga tgtgcagtca acggtaccgg aggcggtttg
      121 tgccgaggat aaggagttga cggctaaacc actaatccct gaagctgaca tttgctaaaa
      181 aatggcaagt ccattttttg gccaacctag cgttagcgaa gtttgggggc gcccttttca
      241 aagggtgcgg ttttattttt tggatatttg catagctaaa gagaagtgct attattgagt
      301 tatcaaataa ataaactgaa aaaagagcaa aaaaataaag tcgagcaacg ctggacacgt
      361 tctcgacctt tacggaaact aatcgcacta gctccgtaac ttcttaatca ttatacgaat
      421 atttttagat ttcttcaaga ctagagattt tttggtaatg aatttttaag agttacgtga
      481 acttttgaaa attctttcca gaagtctctt tttctttgag gaggaacaaa atttgagtaa
      541 aaaaggcacg atcataaatg agtgggaaaa attaacagcc ccctctaaca ggcggttaaa
      601 gtatcaaagt ttgacccttc ccttaaaaat gcagtggtca attgaccgaa ttaccatagt
      661 cggaaaactg aaagaaaata tttattatca cactccaaat gatgtgttga ttttaaattt
      721 tgaacagctt atgcgcctaa atgaaggtaa tggttattta agatctgtta gtaataatgg
      781 ttggcagttg ttagaccaac atgaagagaa tattg
//
LOCUS       DAJSBL010000185          811 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.197, whole genome shotgun sequence.
ACCESSION   DAJSBL010000185 DAJSBL010000000
VERSION     DAJSBL010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..811
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.197"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..672
                     /locus_tag="ONT85_002984"
     CDS             <1..672
                     /locus_tag="ONT85_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR2379877.1"
                     /translation="SGKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDN
                     NKFTSKMEKKLKREQRKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTD
                     FLNKLSTEMIKNHDIICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRK
                     IIKVDKWFPSSQICSECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLH
                     SMGLA"
BASE COUNT          298 a          126 c          152 g          235 t
ORIGIN      
        1 agcggtaaat actatatttc tttgttgtgt aaagaagaaa ttatcgaatt acctaaaact
       61 aattccacaa ttggtattga cttaggtatt atggactttg cgattctttc tgatggacaa
      121 aaaattgata ataataaatt cacgtccaaa atggaaaaga aactaaagcg tgaacaacgc
      181 aaattgtcta ggcgtgcttt attggctaaa aataaaggta ccaatctatt tgaagctaaa
      241 aattatcaaa aacaaaaacg caaagtagct agattgcatg aaaaagtaat gaatcaacgt
      301 actgattttc tgaataagtt gagtacagaa atgatcaaaa atcacgatat tatctgtatt
      361 gaagacttaa acacaaaagg tatgttgcga aatcataaat tagctaaaag tatttctgat
      421 gtttcttggt caaagtttgt gactaaatta caatacaagg ctgactggta tggacgaaaa
      481 atcatcaaag tagataaatg gtttccgtct agtcagattt gttcagagtg cggacataaa
      541 gatggcaaga aatctcttga aattcgagaa tggacttgtc ctgtttgtca tactcatcat
      601 gatcgtgata tcaatgctag tatcaatatt ctgaccgaag gtctaagact acattctatg
      661 ggattggctt agatccaatc atcaagaacc gtaggaacta cggggatagc ttggtaaata
      721 agagacaccg ctgttagtaa agaaatacgc tatcaagtat gctctattcc caagaagctc
      781 ccacttcaag cgttaagaac cgtcaggttt a
//
LOCUS       DAJSBL010000186          788 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.198, whole genome shotgun sequence.
ACCESSION   DAJSBL010000186 DAJSBL010000000
VERSION     DAJSBL010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..788
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.198"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
BASE COUNT          278 a          141 c          107 g          262 t
ORIGIN      
        1 aaccgggggc tttattcctg cagtccggct acgccttcct gcaatagaca aaactccaga
       61 tcgggtaatt cgcttgtggc gaaacccgac ctggagtttg tctctgtatt ttgaacccta
      121 aaaattaatc aaacagactg atattctcat aatcagcgcg aaataagcca ttaagtacag
      181 ttttcctacc tatttttgat aacccgctgg ttagttaata ttccttttcc gagagagata
      241 aatgattggt taaatctata tacatctcaa cctttaaaaa tgttttcgaa atacttcatc
      301 agtacatgtc atacaaatta ccagtgcatt ctcaaattct agttctttaa ttgatttcag
      361 ttgaggagca gttacttttg gcggaaattt ccatttttgt ccaaacctct tcccccgatg
      421 aaatttcttt gttgcaatta ttgcaatgaa aaatttttgt ttcaaaaata tacataatta
      481 cttcctccaa taaaaactat ctacctctca aattattttt ctctaataag aaaaacagaa
      541 aaggtaataa gatacaacag tgatgattcc taataaagaa aagactacaa taatattttt
      601 ctaacttatc gccttcaaag aaatagtttc ttatttttca ctaaaataaa aaatttattg
      661 caaaaaaaat agaagtgaca aaattatcaa tcatttcaag gtaatataac tacctgagtt
      721 tattatcgat taaaatgcta actacatttg caagagaagc gagagttaaa ggtaaatata
      781 gaatttct
//
LOCUS       DAJSBL010000187          774 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.199, whole genome shotgun sequence.
ACCESSION   DAJSBL010000187 DAJSBL010000000
VERSION     DAJSBL010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..774
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.199"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            521..>774
                     /locus_tag="ONT85_002985"
     CDS             521..>774
                     /locus_tag="ONT85_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2379878.1"
                     /translation="MFIKYGADTVATMFTEEYQVDYYEVIDKCVHFVYEKVWNFMNFA
                     ISEEILRVQENRTGIFTRDRKLNLIAHYIRNRWSSIPNFN"
BASE COUNT          304 a           86 c          138 g          246 t
ORIGIN      
        1 aaatgaaaat tttttcgtta gtgtattaat gggtgaaagt ttaatcctaa tactgatttt
       61 gtgtttaatt gttcatgaag tgaataaaca attgtgaatt aaaaaaataa aaacctagta
      121 taatcacttt aataaattgt tggtagaagg tgatcaaaat gggagtaaac gaaatgatga
      181 aagtcagatt gagtttttaa atgatacctt cctcagaact tcaaaacgaa ttcaaagatt
      241 cgttgacaaa tcttgagcaa gatcatttaa aaaaatattt tctcttcatt tgacgaaaaa
      301 tgtttagaaa tcttatataa gtgaagataa agtaattggt gtaattaatt gtagagaata
      361 gcctttgagc aattgaagcg atacaattat ttagaaaatg aacttcctcc agcatactac
      421 atgattcctt acgggacgat gttaaagaag aaagcaagaa attttagaag tattagtaga
      481 taaactaatg ggcttttctc aatttaaggg agtagcccaa atgtttatca aatatggtgc
      541 agatacagta gcaaccatgt ttacagaaga atatcaagta gactattatg aagtaataga
      601 taaatgtgta cattttgttt atgaaaaagt gtggaatttt atgaattttg cgataagtga
      661 agaaatctta cgagtacaag aaaacagaac aggaatattt accagagaca gaaagcttaa
      721 tttgattgcg cattatatac ggaatcgctg gtcatcaata ccaaatttta atta
//
LOCUS       DAJSBL010000188          729 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.200, whole genome shotgun sequence.
ACCESSION   DAJSBL010000188 DAJSBL010000000
VERSION     DAJSBL010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..729
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.200"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..729
                     /locus_tag="ONT85_002986"
     rRNA            <1..729
                     /locus_tag="ONT85_002986"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          180 a          162 c          229 g          158 t
ORIGIN      
        1 tgggatacta cccctgcgtt atggccactc taacccgcac cactaatcgt ggtgggagac
       61 agtgtcagat gggcagtttg actggggcgg tcgcctccta aaaggtaacg gaggcgccca
      121 aaggttccct cagaatggtt ggaaatcatt cgaagagtgt aaaggcagaa gggagcttga
      181 ctgcgagacc aacaagtcga gcagggacga aagtcgggct tagtgatccg gtggttccgc
      241 atggaagggc catcgctcaa cggataaaag ctaccctggg gataacaggc ttatctcccc
      301 caagagtcca catcgacggg gaggtttggc acctcgatgt cggctcgtcg catcctgggg
      361 ctgtagtcgg tcccaagggt tgggctgttc gcccattaaa gcggcacgcg agctgggttc
      421 agaacgtcgt gagacagttc ggtccctatc cgtcgcgggc gttggaaatt tgagaggagc
      481 tgtccttagt acgagaggac cgggatggac ttaccgctgg tgtaccagtt gttctgccaa
      541 gggcattgct gggtagctat gtagggaagg gataaacgct gaaagcatct aagtgtgaag
      601 cccacctcaa gatgagattt cccatttctt taagaaagta agatccctga gagatgatca
      661 ggtagatagg tcaggagtgg aagtacagtg atgtatggag cggactgata ctaatcgatc
      721 gaggactta
//
LOCUS       DAJSBL010000189          712 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.202, whole genome shotgun sequence.
ACCESSION   DAJSBL010000189 DAJSBL010000000
VERSION     DAJSBL010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..712
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.202"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..446)
                     /locus_tag="ONT85_002987"
     CDS             complement(<1..446)
                     /locus_tag="ONT85_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR2379879.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYISVENFAKQHHVSYSVAYKVLQRLKRNLKKYQIFFEKRK"
BASE COUNT          216 a          123 c           95 g          278 t
ORIGIN      
        1 aactttctct tttcaaaaaa aatttgatat ttttttagat ttctttttag tctttgtaat
       61 actttataag ccacagaata actcacgtga tgctgtttag caaaattttc tactgagata
      121 tatttttcct gaaagaaagc caataccatt ttcatagata aagaatccgt tatataacga
      181 gataacagag ctttgctaga acattttcca ttaatttcca gacatatatc caattcactg
      241 acggttactc gcaaattttt ctctagatcg aacttttgta aatcatgttt cagcttatcg
      301 atcgtttttt tcaagagaaa tgtcgatagc gacaactctt cttgaacatt cgatattgac
      361 tctgataaat ttgagcttgc ttctaaatat ttaagtaatg aataacttct gtgatcttct
      421 ttgcttaaaa aaaactcata attcattagt catcagctct ttctacgtat attactttcc
      481 atgcattcac caactagctg gggataaaac tgatttcctg gatccgagtc actgttgtca
      541 gcgattcttg tttgtataat atacatcgtc ctgtcttttt tttaacattt tggagagcgt
      601 tttatttggc ctttctcgcg atcaaacctg ttattagaat tatatcattt actagtatgc
      661 atttttatta ataaaatggt atattttata ttgttataaa aagagcccaa cc
//
LOCUS       DAJSBL010000190          696 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.204, whole genome shotgun sequence.
ACCESSION   DAJSBL010000190 DAJSBL010000000
VERSION     DAJSBL010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..696
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.204"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(54..629)
                     /locus_tag="ONT85_002988"
     CDS             complement(54..629)
                     /locus_tag="ONT85_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HCR2379880.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
BASE COUNT          230 a          151 c          124 g          191 t
ORIGIN      
        1 aatgaaaaaa cagagccaga ttagcattat tttgctaatc cagctctgtt tattcagcct
       61 atttttcttt ttgaatatat caagcgttgg ggcaacatag tgatgactaa tgaaacaacg
      121 acgatagaca acaagcgatc aaggtagtct gtacccgcct gtaccaagat cacacttgtt
      181 gtctgactta aacctaaccc atgtaatagc tgaacgatca tactggatcc tgaggaggtg
      241 atcccgccaa ataactgaac ggtaatgatc gaagaaacga tcgttccagg taccgacaaa
      301 atcaaagcga gtaataacag tttcaagctg tttttatctt tgccgagata tcttggcaaa
      361 atcaatccag caagcaagcc tgtgagaatt tggattggcg aataatacag agaaaaaata
      421 tctgtagtca ctccacttaa aataccgctg gacaatcctg tcagcattcc aggtacaggc
      481 ccaagcaatg caccagcaaa aatcgttccg atagaatcga gatagattgg caaacgaagc
      541 cataaagcaa tcgttcctcc aatataattg atcccaatac acaaagccat cactgtaagt
      601 acgcgagttg ccattttgtt tttattcatt ttgttagccc atctttaacc tttccaaatc
      661 tttcataatc gtttcttctt gtgcatttaa aagaac
//
LOCUS       DAJSBL010000191          649 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.205, whole genome shotgun sequence.
ACCESSION   DAJSBL010000191 DAJSBL010000000
VERSION     DAJSBL010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..649
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.205"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..>649
                     /locus_tag="ONT85_002989"
     CDS             <1..>649
                     /locus_tag="ONT85_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HCR2379881.1"
                     /translation="KSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPISCSA
                     RTIYRMFKKGLFDSSDLPMKGKRKPNGHQERRGKQTFRRSIHEREKDYSQFSNEFGHL
                     EGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIEKKLNQWFESVPKNLFKSIT
                     FDCGKEFSNWKQISNVNDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNSV"
BASE COUNT          213 a          111 c          134 g          191 t
ORIGIN      
        1 aaatcaaact gtggtagacg tccgcttgtt ttacccgagg aacaatcaga atatattcaa
       61 agaaaggttg ttcaaggatg gacacccgat gtgattgttg gtcgtgcagc gtttcctatt
      121 agttgttctg ctcgtaccat ttatcgtatg ttcaaaaagg ggctatttga ttcttctgac
      181 ttaccgatga aaggcaagcg taaaccgaat ggacatcaag aaagacgtgg aaaacaaact
      241 ttccgccgct ctattcatga acgtgaaaag gattatagcc aattctcaaa tgagtttggt
      301 caccttgaag gtgacactat cgtaggtctg aaacataaaa gtgctgtaat taccttagtt
      361 gaacgattat caaaagttat catcacattg aaaccgtgtg gtagacaagc gattgatatt
      421 gaaaaaaaat taaatcaatg gtttgaatct gtaccgaaaa acctattcaa atccatcact
      481 tttgattgtg gaaaggaatt ttcaaattgg aaacagatca gtaatgtcaa tgatattgcc
      541 atttatttcg ctgatccagg aacgccgtct caaagaggcc taaacgagaa ttctaacgga
      601 ttgttacgta gagatggttt attgaaatct atggatttca attcagtag
//
LOCUS       DAJSBL010000192          635 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.206, whole genome shotgun sequence.
ACCESSION   DAJSBL010000192 DAJSBL010000000
VERSION     DAJSBL010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..635
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.206"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..270)
                     /locus_tag="ONT85_002990"
     CDS             complement(<1..270)
                     /locus_tag="ONT85_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2379882.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFR"
     gene            complement(276..>635)
                     /locus_tag="ONT85_002991"
     CDS             complement(276..>635)
                     /locus_tag="ONT85_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HCR2379883.1"
                     /translation="RNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPP
                     RIDTKKLNMLETILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQC
                     ALMERAIAAKRKELDKK"
BASE COUNT          164 a          135 c          110 g          226 t
ORIGIN      
        1 acggaagtag aagaattcct tccagaagtc cgttggcatg cctgtatcga taaaaatatc
       61 gcctatcagc gcatagatga agtttcgttg ctgtacggtg aaacgtctag gatcaataaa
      121 acgaatttca atgactcgat caccatcgta gccgtcatac atcgtcttta gtcgatcaat
      181 gttcacttcc tcattgatgg ttgcgcttat gtcttttcct ttgaactttt tcagaaccgc
      241 tgaatatgaa tcgattaatg gtttaaacac tcatatcact tcttatctaa ttcttttctc
      301 ttagctgcta ttgctcgctc catcaaggca cattgctcat agcttaactg ttcaatagtt
      361 tcaacgttat cagctaagag ccctaattta tctgtctgct cattaacata ttcgattaag
      421 gttttggtca tatctttacc catctgctca ttgaaagctt ctagaatcgt ctctagcatg
      481 tttaatttct ttgtatcgat tctaggtggt gttggaatat cttccccttg aaatacatat
      541 aatcccagtc cgtgtagagc caatgctttc acaaagcatc gcttcaatga gttattgatt
      601 tgcattgcat ttggtttaac aactggttgg tttcg
//
LOCUS       DAJSBL010000193          555 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.207, whole genome shotgun sequence.
ACCESSION   DAJSBL010000193 DAJSBL010000000
VERSION     DAJSBL010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..555
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.207"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            15..194
                     /locus_tag="ONT85_002992"
     CDS             15..194
                     /locus_tag="ONT85_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucocin A/sakacin P family class II
                     bacteriocin"
                     /protein_id="HCR2379884.1"
                     /translation="MLGTCLAGIGTGIDVDAATYYGNGLYCNKEKCWVNWGQSWSEGL
                     KRWGDNLFGSFSGGR"
     gene            194..544
                     /locus_tag="ONT85_002993"
     CDS             194..544
                     /locus_tag="ONT85_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HCR2379885.1"
                     /translation="MTSINIDHTKKVIHELYNSLMTRPNQTKALLDITDVLFQVYKKI
                     DTAKYPEILVNKLVNYIYIVGFDNKIRFIDNDEKLLIELGDISKKAGINSKYKANFTD
                     KSQFYSYSETMPQR"
BASE COUNT          217 a           61 c          100 g          177 t
ORIGIN      
        1 gtgttattct aggaatatta ggaacttgtc tagctggcat tggtacagga atagacgttg
       61 acgcagctac ttactatgga aatggtcttt attgtaacaa agaaaaatgt tgggtaaatt
      121 ggggacaatc atggtcagaa gggttaaaac gttggggaga taatcttttt ggtagtttta
      181 gtggaggacg ataatgactt caataaacat tgatcacaca aaaaaagtca tacatgaatt
      241 atacaatagt ttaatgacac gacctaatca gacgaaagca ttattagata ttactgatgt
      301 tttatttcag gtatataaaa aaattgatac agcaaaatat cctgaaattc ttgtaaataa
      361 attagttaat tatatttaca tcgtgggttt tgataataaa atacgcttta ttgataatga
      421 tgaaaaatta ttaatagaat taggggatat ttctaaaaaa gctggaatta atagtaaata
      481 caaagcaaac tttacagata aatcgcaatt ttatagttat tcagaaacaa tgcctcaacg
      541 ataaaatata gtata
//
LOCUS       DAJSBL010000194          528 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.208, whole genome shotgun sequence.
ACCESSION   DAJSBL010000194 DAJSBL010000000
VERSION     DAJSBL010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..528
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.208"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(5..90)
                     /locus_tag="ONT85_002994"
     tRNA            complement(5..90)
                     /locus_tag="ONT85_002994"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(54..56),aa:Leu,seq:taa)
     gene            complement(107..178)
                     /locus_tag="ONT85_002995"
     tRNA            complement(107..178)
                     /locus_tag="ONT85_002995"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(144..146),aa:Gly,seq:gcc)
     gene            complement(190..262)
                     /locus_tag="ONT85_002996"
     tRNA            complement(190..262)
                     /locus_tag="ONT85_002996"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(227..229),aa:Thr,seq:tgt)
     gene            complement(280..361)
                     /locus_tag="ONT85_002997"
     tRNA            complement(280..361)
                     /locus_tag="ONT85_002997"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(325..327),aa:Leu,seq:tag)
     gene            complement(369..441)
                     /locus_tag="ONT85_002998"
     tRNA            complement(369..441)
                     /locus_tag="ONT85_002998"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(406..408),aa:Lys,seq:ttt)
     gene            complement(445..517)
                     /locus_tag="ONT85_002999"
     tRNA            complement(445..517)
                     /locus_tag="ONT85_002999"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(482..484),aa:Val,seq:tac)
BASE COUNT          135 a          152 c          128 g          113 t
ORIGIN      
        1 gaaatgccga ggaccggaat cgaaccggta cggtgatcac tcaccgcagg attttaagtc
       61 ctgtgcgtct gccagttccg ccaccccggc gcgattgctt tggcaaagcg gaaaacgggg
      121 ttcgaacccg cgacccccac cttggcaagg tggtgctcta ccactgagct atttccgcgt
      181 gtttaaagat gccggctaaa ggacttgaac cctcgaccct ctgattacaa atcagatgct
      241 ctaccaactg agctaagccg gcatgtaaaa cttaaattat gcgggtgaag ggacttgaac
      301 ccccacgccg taaggcgcta gatcctaaat ctagtgcgtc tgccaattcc gccacacccg
      361 caaaaatatg agccgtacag ggctcgaacc tgtgaccctc tgattaaaag tcagatgctc
      421 taccaactga gctaacggct catatggagg ttaacgggat cgaaccgctg accctctgct
      481 tgtaaggcag atgctctccc agctgagcta aacctccatg gataactt
//
LOCUS       DAJSBL010000195          526 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.209, whole genome shotgun sequence.
ACCESSION   DAJSBL010000195 DAJSBL010000000
VERSION     DAJSBL010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..526
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.209"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(6..359)
                     /gene="tnpB"
                     /locus_tag="ONT85_003000"
     CDS             complement(6..359)
                     /gene="tnpB"
                     /locus_tag="ONT85_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCR2379886.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
     gene            complement(349..>526)
                     /locus_tag="ONT85_003001"
     CDS             complement(349..>526)
                     /locus_tag="ONT85_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2379887.1"
                     /translation="IVPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIYNGINNY
                     ILQAILKELLSNAL"
BASE COUNT          153 a          116 c           90 g          167 t
ORIGIN      
        1 ccagcttaaa aaactccttt ttttcctggc aaaatagctc gaggttgatc gatacttaat
       61 ccttctaaca accatcgaac ctgttggttc gttaaggttc gaacttcgtc tttcgtccga
      121 ggccatttta acttgccgtt ttctatccgc ttataacaga gaagaaaacc atcaccgtcc
      181 caatagagaa gtttgaaacg gtcttgacgg ttcccgcaaa ataaaaaaac ggcatcttcg
      241 taaagctcga gttgatactc ttgttgaata agactggcaa gaccatcaat tccttttcgc
      301 aaatctgttt ttccgcacac aatataaata tgtttggttt gaagtaaatc ataaagcatt
      361 ggacaacagt tctttcaaaa tagcttgaag aatataattg ttaataccgt tatagatcaa
      421 aacttcagaa ccattctctc ttttgatgcg catcgctaac ttggcagccg cgggtttacc
      481 tttttctggt gcactctttt cgtttcctaa ttggacagga acaatc
//
LOCUS       DAJSBL010000196          524 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.210, whole genome shotgun sequence.
ACCESSION   DAJSBL010000196 DAJSBL010000000
VERSION     DAJSBL010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..524
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.210"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            1..524
                     /locus_tag="ONT85_003002"
                     /pseudo
     CDS             1..524
                     /locus_tag="ONT85_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS1485 family transposase"
BASE COUNT          218 a           91 c           95 g          120 t
ORIGIN      
        1 gtaaagcaat ctaaaacgag tggttcattc aaagaaaaag ataatcttac gcctgaacaa
       61 aaagaattgt tagaactacg taaaagaaac cagcaattag aaatggaaaa tgatatttta
      121 aagcaagcag cgctgatatt cggacgaaga gacaagtaat cgatgcgaat aagcatcttt
      181 accctatatc agcgatgtgc agaatattag gtctatcacg tcagtcctat tattatcaat
      241 caaaaccaaa gaaagacgaa tcagaacttg aagaagtagt cgctgaagaa tttatccgca
      301 gccgaaaggc ctacggctca agaaaaataa aaaaagcctt atcaaaacga ggcattcaga
      361 tcagccgacg aaaaattagt agaatcatga aaaatagagg attaaaatcg agctatactg
      421 ttgcttattt taaagtacat cattctactt gcaatgaagc caaaacgaca aacgtattga
      481 atcgtaaatt cttaagagac aacccattag aagcgatcgt aaca
//
LOCUS       DAJSBL010000197          515 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.211, whole genome shotgun sequence.
ACCESSION   DAJSBL010000197 DAJSBL010000000
VERSION     DAJSBL010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..515
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.211"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(107..>515)
                     /locus_tag="ONT85_003003"
     CDS             complement(107..>515)
                     /locus_tag="ONT85_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HCR2379888.1"
                     /translation="GTPENHFSNQISLNVYSRGMGGLGLPGDLSSVSRFVKATFTKMN
                     AASGDSESESISQFFHILGSVEQQKGVCDTGEGKYEYTIYSSCCNVDKGIYYYRTYED
                     SQITAVNMNKEDLDRHELVSYPVIKEQQINYVN"
BASE COUNT          163 a          103 c           78 g          171 t
ORIGIN      
        1 atcatccata aaatggactt tgtctacagt ctgagacacg acctaaagat cgtgtctaat
       61 tatgactgta tcagaaaata tgataccaaa cggatctatt cattaattaa ttgacatagt
      121 ttatctgttg ctcttttatg actggataac tgaccaattc atgacgatcc aagtcttctt
      181 tattcatatt tacagcagta atttgactgt cttcatacgt acgataataa taaatccctt
      241 tatcaacgtt acagcaagaa gaataaatcg tatattcgta ttttccttct ccagtatcgc
      301 atactccttt ttgttgctcg actgagccta gtatatgaaa aaattgactg atactttcag
      361 attctgaatc acctgatgca gcattcattt tagtaaatgt ggcttttaca aatcgagaaa
      421 cagaagagag atctccaggt aagcccaatc ctcccatccc acgactataa acattcaagc
      481 tgatctggtt tgaaaaatga ttttctggtg ttcca
//
LOCUS       DAJSBL010000198          509 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.212, whole genome shotgun sequence.
ACCESSION   DAJSBL010000198 DAJSBL010000000
VERSION     DAJSBL010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..509
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.212"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
BASE COUNT          228 a           70 c           82 g          129 t
ORIGIN      
        1 gaattcctta ggcagctttt gctgtgtaga aaggaataaa taataacgaa agttcaaaag
       61 ctaatgccga ctattctaga cattaaagaa gacgaaaacg aacgataaag aaagaaaagt
      121 tatgaataat cgtttattga aaaaaacttg aaaaaataca aaaaattcgt tgaccaagct
      181 aaatttagtt ggtatattat tacttgtcca aaagacagaa gaaaatattt attcaaaaac
      241 ataacaaaaa agttattgac atgttactaa cgacatgata agataactga gtcgttaaaa
      301 aagaaccact tgcgaagctt gattctatcg aaaaaaactt caaaaaagtt cttgacaaat
      361 aacaaacgat aagttatgat aaatgagttg ccgaaagatt tcgaaaaacg aaatcgaaaa
      421 aaacttttaa aaaagagttg acaatcgctt gtctctctga tatgatataa aagttgctac
      481 ggcagccaaa aatgaatcgg gaaacactc
//
LOCUS       DAJSBL010000199          509 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.213, whole genome shotgun sequence.
ACCESSION   DAJSBL010000199 DAJSBL010000000
VERSION     DAJSBL010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..509
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.213"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..132)
                     /locus_tag="ONT85_003004"
     CDS             complement(<1..132)
                     /locus_tag="ONT85_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HCR2379889.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
BASE COUNT          183 a           93 c           91 g          142 t
ORIGIN      
        1 tcgtatgaat gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa
       61 ttgattgtcg tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac
      121 aaataatttc atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac
      181 tgtgggattt cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat
      241 aaaagagcta gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca
      301 gctaatgagt tggtagtgat aacagtggtt cgggtaacag actaaacagg gtagacaaac
      361 gctacaggag gaaatagcaa attcccgttg gatcctttcc atacatccat tgtactctga
      421 gaaatcacac aatatcgaaa caaattccgt taggaatgtg ttattcaata aattttataa
      481 tctagaaaca ttttgaataa aaactatta
//
LOCUS       DAJSBL010000200          498 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.214, whole genome shotgun sequence.
ACCESSION   DAJSBL010000200 DAJSBL010000000
VERSION     DAJSBL010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..498
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.214"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            141..398
                     /locus_tag="ONT85_003005"
     CDS             141..398
                     /locus_tag="ONT85_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2379890.1"
                     /translation="MSNLENKEEKVVNKIVSVVNKLDKELDELDTLSENPEKKHNLKK
                     WLVERKAIHEIKKVLHEADKYEKYDEKELDKEFKEINDLLL"
BASE COUNT          210 a           65 c           70 g          153 t
ORIGIN      
        1 ttctgtccac gttcatttta ctccaaagtt ttcctcattt caaaagaaaa tcacagaata
       61 cacgaagaag cgtttctatt tgtataaact atatcaaagt tctatactta aattatcaaa
      121 taaaaaggac gtgaagataa atgtcaaatt tagaaaacaa agaagaaaaa gttgtaaaca
      181 aaattgtttc tgttgtcaat aaactggata aagagttaga cgaacttgat acgttatctg
      241 aaaatccaga aaaaaaacat aacctaaaaa aatggttagt tgaacgaaag gctattcatg
      301 aaattaaaaa agttcttcat gaagctgata aatatgaaaa atatgatgaa aaagaacttg
      361 ataaagaatt taaagaaatc aatgacttat tactataatt gatttatacg actcattttt
      421 gagtcgtcag actgcaaaca acactctaaa tttaggagtt tgtctacagt ctgaatagat
      481 tttaaattta ttgtccat
//
LOCUS       DAJSBL010000201          487 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.215, whole genome shotgun sequence.
ACCESSION   DAJSBL010000201 DAJSBL010000000
VERSION     DAJSBL010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..487
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.215"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..>487)
                     /locus_tag="ONT85_003006"
     CDS             complement(<1..>487)
                     /locus_tag="ONT85_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HCR2379891.1"
                     /translation="IHRRFYTSICHQKITTDTTEFKYHEVDAKGIIRQKKLYLDPFMD
                     MFNSEILSYRISEKPNALAVMEGLEEAIQTTNDCPYRRTFHSDQGWAYQMKAYRNKLK
                     EYKIFQSMSRKGNCLDNSLMENFFGLLKQEIFHGKVYNCFEELKSAIDSYIYYYNNER
                     IK"
BASE COUNT          142 a           92 c           86 g          167 t
ORIGIN      
        1 gttttatccg ttcattattg tagtaataga tatagctatc gatggctgat tttaattcct
       61 cgaaacagtt ataaactttc ccgtgaaaaa tctcttgttt taataagcca aaaaaattct
      121 ccataagtga attatctaag cagttccctt tccgagacat gctttgaaag attttgtact
      181 ccttcaattt gtttctataa gctttcattt gatacgccca accttgatct gagtgaaacg
      241 tacgacgata gggacaatcg ttagtggtct ggatagcttc ctccaaacct tccattaccg
      301 ctaatgcatt gggcttttca gagatacgat aggataaaat ctcactattg aacatatcca
      361 taaaaggatc tagatacaat ttcttttgac ggataatgcc tttggcatcc acttcatggt
      421 atttaaattc agttgtatcc gtcgtgattt tctgatggca gatactggtg tagaaacgac
      481 gatggat
//
LOCUS       DAJSBL010000202          483 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.216, whole genome shotgun sequence.
ACCESSION   DAJSBL010000202 DAJSBL010000000
VERSION     DAJSBL010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..483
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.216"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            93..209
                     /locus_tag="ONT85_003007"
     CDS             93..209
                     /locus_tag="ONT85_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010768364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1972 domain-containing protein"
                     /protein_id="HCR2379892.1"
                     /translation="MIGLNAISAKYCKFKAFVEKFTERQTDKIIQYHITYTD"
BASE COUNT          207 a           67 c           64 g          145 t
ORIGIN      
        1 actaattaac aaaagaattt gaattgttaa taaaatccaa taaaatatat taataaagta
       61 gatagaaata aaaaaacaaa gactaaacat atataattgg attaaacgca atatctgcaa
      121 aatattgtaa atttaaagcc tttgtagaaa aatttactga aagacaaact gataagatta
      181 ttcagtatca tattacctat actgattaaa attttttgaa acgggatact aaataaaaaa
      241 tttatttata acagaactaa ttatttaatg cggatgcgcc aatacttggt cctgcaagag
      301 ctatttattt tgatcgccgt ttgtataatt aagcgagaca aataaaaaag ccatttatgt
      361 tacactcaaa ttgtaatacc cgctaaagca tacaaggaga gtgaacataa atgacttata
      421 cccatcttac accaaacgag cttgtaatga tagaagcata ttttcatcaa gaaactccgg
      481 ttg
//
LOCUS       DAJSBL010000203          460 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.217, whole genome shotgun sequence.
ACCESSION   DAJSBL010000203 DAJSBL010000000
VERSION     DAJSBL010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..460
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.217"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(20..91)
                     /locus_tag="ONT85_003008"
     tRNA            complement(20..91)
                     /locus_tag="ONT85_003008"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(56..58),aa:Glu,seq:ttc)
     gene            complement(113..200)
                     /locus_tag="ONT85_003009"
     tRNA            complement(113..200)
                     /locus_tag="ONT85_003009"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(164..166),aa:Ser,seq:gct)
     gene            complement(225..298)
                     /locus_tag="ONT85_003010"
     tRNA            complement(225..298)
                     /locus_tag="ONT85_003010"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(262..264),aa:Ile,seq:gat)
     gene            complement(312..382)
                     /locus_tag="ONT85_003011"
     tRNA            complement(312..382)
                     /locus_tag="ONT85_003011"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(348..350),aa:Gly,seq:tcc)
     gene            complement(384..459)
                     /locus_tag="ONT85_003012"
     tRNA            complement(384..459)
                     /locus_tag="ONT85_003012"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(424..426),aa:Asp,seq:gtc)
BASE COUNT          132 a          128 c          108 g           92 t
ORIGIN      
        1 taaaactata ttaaattaat gacccgtacg ggattcgaac ccgtgttacc gccgtgaaag
       61 ggcggtgtct taaccgcttg accaacgggc caggtaaaac aaaatattga aacggagaag
      121 gagggatttg aaccctcgcg ccggtttccc gacctacacc cttagcaggg gcgcctcttc
      181 agccacttga gtacttcccc aaaaccaata agattttgta ctaatgggcc taaatggact
      241 cgaaccatcg acctcacgct tatcaggcgt gcgctctaac cagctgagct ataggcccat
      301 aaaaaactaa aagcgggtga cgagaatcga actcgcgaca acagcttgga aggctgtggt
      361 tttaccacta aactacaccc gcatggcggt tccgacggga atcgaacccg cgatctcctg
      421 cgtgacaggc aggcatgtta acccctacac cacggaacca
//
LOCUS       DAJSBL010000204          436 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.218, whole genome shotgun sequence.
ACCESSION   DAJSBL010000204 DAJSBL010000000
VERSION     DAJSBL010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..436
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.218"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            227..424
                     /locus_tag="ONT85_003013"
     CDS             227..424
                     /locus_tag="ONT85_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HCR2379893.1"
                     /translation="MNNGTVKWFNSDKGFGFITGEDGNDVFAHFSAIQGEGFKSLDEG
                     QAVTYDIEEGQRGPQAVNIVK"
BASE COUNT          158 a           61 c           78 g          139 t
ORIGIN      
        1 ttacgtaaat ggaaagtttt gtctcgctta atttgacaaa taataatatt taaaccgtat
       61 atcgagtgat tatccttgtg ttagcattac atccgtgtta tagtaaataa gtaatctaat
      121 ttgaaacgta atctgagcga tatattcaca ctataaaaac tccttttacc aagtaatatt
      181 aattgcaata aaacacgtat tatatacgta tcaggaggaa atatatatga ataacggtac
      241 agtaaaatgg tttaactcag ataaaggttt tggtttcatc actggtgaag atggaaatga
      301 cgtattcgct catttctcag caatccaagg agaaggcttt aagtctttag atgaaggcca
      361 agcagttact tatgatattg aagaaggaca acgtggtcct caagcagtaa atatcgtaaa
      421 ataatgttga acgtta
//
LOCUS       DAJSBL010000205          415 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.219, whole genome shotgun sequence.
ACCESSION   DAJSBL010000205 DAJSBL010000000
VERSION     DAJSBL010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..415
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.219"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..339)
                     /locus_tag="ONT85_003014"
     CDS             complement(<1..339)
                     /locus_tag="ONT85_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR2379894.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYT"
BASE COUNT          116 a           70 c           68 g          161 t
ORIGIN      
        1 tgtataactc tgaacaggat ttttcttact tttgaaacgt ggaaatccaa cggatttatc
       61 ccgaaaaaaa ttcttgtatg ctttatctaa attaagttgg gcattggcta aagcaaggct
      121 atcaatttct ttcaaaaacg gaaattcttt cttgtattta gctggtgtcg gaaaagtcat
      181 ttttttagaa gaatcatttt taacttcttc atacgccttt tttcggtcat caagcattag
      241 attgtagacc ttacggacac aaccaaaaga tttggcaaag aagatttctt gttcctctgt
      301 tggatagatt ctgaatttgt atgcttttag ttgttccata aagttcacct actttctatt
      361 tatgattgtt tccttgattt tggatatact tcttaatcac atcaattggt gcgcc
//
LOCUS       DAJSBL010000206          413 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.220, whole genome shotgun sequence.
ACCESSION   DAJSBL010000206 DAJSBL010000000
VERSION     DAJSBL010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..413
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.220"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..390
                     /locus_tag="ONT85_003015"
     CDS             <1..390
                     /locus_tag="ONT85_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR2379895.1"
                     /translation="KDRKEICDDFKAVYQANSKEEANTFLSGMIEKWKKNYPKVTQSL
                     IENQDLLTFYDFPPSIRRTIYSTNLIESFNKQIKRYSRRKEQFQNEESLERFLVSIFD
                     TYNQKFLNRSHKGFQQVTDTLVSMFTE"
BASE COUNT          161 a           74 c           65 g          113 t
ORIGIN      
        1 aaagaccgaa aagaaatctg tgatgacttt aaggctgttt atcaagctaa ctcaaaagaa
       61 gaagcgaata ccttcttatc cggcatgatt gagaaatgga agaaaaacta tcctaaagtg
      121 acgcagtcac tcatagaaaa ccaagactta ttaacttttt atgattttcc acctagcatt
      181 cgtagaacca tttactcaac caatctaatc gagtctttca ataagcaaat taaaagatac
      241 agccgtagaa aagagcagtt tcaaaatgaa gaatcactag aacgctttct agtcagcatt
      301 tttgatacat acaatcaaaa atttctaaac agaagccata aaggttttca acaggtaacc
      361 gatacattag tttcaatgtt tactgagtaa ctaattattt tgcaggagga caa
//
LOCUS       DAJSBL010000207          409 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.221, whole genome shotgun sequence.
ACCESSION   DAJSBL010000207 DAJSBL010000000
VERSION     DAJSBL010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..409
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.221"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..379
                     /locus_tag="ONT85_003016"
     CDS             <1..379
                     /locus_tag="ONT85_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="HCR2379896.1"
                     /translation="IWLRKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKL
                     KEYGFYQGTEHRTIKYLNNLIEQDHRPVKRRNKFYRSLRTASTTIKGMEAIRGLYKKT
                     RKEGTLFGFSVCTEIKVLLGIPA"
BASE COUNT          139 a           79 c           81 g          110 t
ORIGIN      
        1 tatttggtta cgtaaaaaac gggacacaca agcagcctat gcttttctta agcggttagt
       61 gaagcagttt gatgaaccga aggttgtagt cacagataaa gccccctcta ttacaagtgc
      121 ctttaagaaa ctaaaagaat acggctttta tcaagggaca gaacatcgta ccattaaata
      181 cctgaataat ttgattgaac aagaccatcg tccagtaaag agacgcaata aattctatcg
      241 aagtttacgc actgcctcta ccacgattaa aggcatggaa gccattcgag gattatataa
      301 gaaaacccga aaagaaggca ctctcttcgg gttttcggtc tgtactgaaa tcaaggtatt
      361 attgggaatc ccagcttaaa tcatagatac cgtaagggat tttattctt
//
LOCUS       DAJSBL010000208          404 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.222, whole genome shotgun sequence.
ACCESSION   DAJSBL010000208 DAJSBL010000000
VERSION     DAJSBL010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..404
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.222"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..>404
                     /locus_tag="ONT85_003017"
     CDS             <1..>404
                     /locus_tag="ONT85_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2379897.1"
                     /translation="PEDKLPVLKAKDGYTDAKWPEEATQPITADDTEFVSSATKLDDK
                     SDADKYNPEGQKVTTELNKEPDAFEGIKNKEDLPKDTKYTWKEKVDVSAAGNKKGTVV
                     VTYPDGSSDEVEVDVTVTDNRSDADKYEPTVEG"
BASE COUNT          166 a           60 c           92 g           86 t
ORIGIN      
        1 ccagaagata aacttccagt gttgaaagca aaagatggtt atacagatgc gaaatggcca
       61 gaggaagcaa ctcaaccaat tacagcagat gatacagaat ttgtatcaag tgcaacaaaa
      121 ctggatgata aatctgacgc tgacaaatat aatcctgaag gtcaaaaagt gactacagaa
      181 ttgaataagg aaccagacgc atttgaggga attaagaaca aagaagattt accaaaagat
      241 actaagtata cttggaaaga aaaagtagat gttagcgcag ctggaaataa aaaagggact
      301 gttgtagtga catatccaga tggatcatct gatgaagttg aagtagatgt tacagtgaca
      361 gacaatcgtt ctgacgccga taaatatgag ccaacagtag aagg
//
LOCUS       DAJSBL010000209          381 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.223, whole genome shotgun sequence.
ACCESSION   DAJSBL010000209 DAJSBL010000000
VERSION     DAJSBL010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..381
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.223"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(21..94)
                     /locus_tag="ONT85_003018"
     tRNA            complement(21..94)
                     /locus_tag="ONT85_003018"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(58..60),aa:Ile,seq:cat)
     gene            complement(108..181)
                     /locus_tag="ONT85_003019"
     tRNA            complement(108..181)
                     /locus_tag="ONT85_003019"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(145..147),aa:Met,seq:cat)
     gene            complement(209..281)
                     /locus_tag="ONT85_003020"
     tRNA            complement(209..281)
                     /locus_tag="ONT85_003020"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(246..248),aa:Ala,seq:tgc)
     gene            complement(301..374)
                     /locus_tag="ONT85_003021"
     tRNA            complement(301..374)
                     /locus_tag="ONT85_003021"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(338..340),aa:Pro,seq:tgg)
BASE COUNT          103 a          108 c           90 g           80 t
ORIGIN      
        1 tgaaacttac aacgccacaa tggaccttgt aggactcgaa cctacgaccg gacggttatg
       61 agccgtctgc tctaaccaac tgagctaaag gtccaggctc ttaaaaatcg cggcggaggg
      121 gatcgaaccc ccgacctccc gggtatgaac cggacgctct agccagctga gctacaccgc
      181 gaaaaagata ttcattattt aaatgaaatg gagcctagcg ggatcgaacc gctgacctcc
      241 tgcgtgcaaa gcaggcgctc tcccagctga gctaaggccc cgtgtttaaa tttataatta
      301 tcgggaagac aggattcgaa cctgcgaccc cttggtccca aaccaagtgc tctaccaagc
      361 tgagctactt cccgtttaat t
//
LOCUS       DAJSBL010000210          343 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.225, whole genome shotgun sequence.
ACCESSION   DAJSBL010000210 DAJSBL010000000
VERSION     DAJSBL010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..343
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.225"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
BASE COUNT          126 a           57 c           54 g          106 t
ORIGIN      
        1 attagcgatg cagggatcga gagaaacatc aaaagggtat ccttcaagat cacgttaaac
       61 aagcctaccg ataaaaagct ctcagaagcg ttctgagagc ttttgacatt ttcttgtata
      121 attttatcct aacacattac aaagcaccta attcgagctt atatcaagca aatttgctat
      181 ttattactca atacatatct aaaaattact tgatggaaaa ttagatacat ggtaagcatg
      241 aaaagaaaat caatttgagc agatgcagta caatacatag caatttaaaa gctctcagaa
      301 ggcttctgag agcttttaat atattctttg ataatttgtc ttt
//
LOCUS       DAJSBL010000211          341 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.226, whole genome shotgun sequence.
ACCESSION   DAJSBL010000211 DAJSBL010000000
VERSION     DAJSBL010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..341
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.226"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..>341
                     /locus_tag="ONT85_003022"
     CDS             <1..>341
                     /locus_tag="ONT85_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HCR2379898.1"
                     /translation="RLPDGSYHLSGNDFVDLYQRATRATNAKRNADNMSEQYLQMLEQ
                     NTELNEYVATLEEERHLSGRKQLADLQVENKKLKEENRSLRQRLERLKNAFQKSITRL
                     SIRFGLAEKDMG"
BASE COUNT          120 a           50 c           76 g           95 t
ORIGIN      
        1 aggcttcctg atggttcgta tcatttatct gggaatgatt ttgttgattt gtatcaacga
       61 gctacgagag caacaaatgc caagagaaat gctgataaca tgagtgagca atatttacag
      121 atgcttgagc aaaatacaga attaaatgag tatgtagcta cattggaaga agagcgtcat
      181 ttgtctggta gaaaacaatt agcagattta caagtggaaa ataaaaagct gaaagaagaa
      241 aatcgctctt tacgccagcg tttagagcga ttaaaaaacg catttcaaaa atcgatcact
      301 cgtttgtcta tccgttttgg tcttgcagaa aaggatatgg g
//
LOCUS       DAJSBL010000212          337 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.denovo.227, whole genome shotgun sequence.
ACCESSION   DAJSBL010000212 DAJSBL010000000
VERSION     DAJSBL010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..337
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.denovo.227"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..321)
                     /locus_tag="ONT85_003023"
     CDS             complement(<1..321)
                     /locus_tag="ONT85_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HCR2379899.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHL"
BASE COUNT           95 a           54 c           61 g          127 t
ORIGIN      
        1 taaatggata atagtggttt ccaatgtatc gtttgttctt ttgtaggctg gtaaagtttg
       61 ttgtttaaac tcaccattac gatctctagg tatttccaat gttaattcac catattcggt
      121 tttgattgat cgaaagtaag aaccgtttct cgaattacct gaattaaaac cagtgcgatc
      181 atatttttcg taatctaaaa aagccgttaa ttcagtccgt aggagtgtgt ttatcgcttt
      241 ttctaagtgc gaacggaata attcatttaa atcgccttta gtgactagag tttgcacaat
      301 ttctgtagta aaatcattca tagggaagtc ctctttt
//
LOCUS       DAJSBL010000213        19019 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.guided.052, whole genome shotgun sequence.
ACCESSION   DAJSBL010000213 DAJSBL010000000
VERSION     DAJSBL010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19019
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.guided.052"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     misc_binding    25..241
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            289..2934
                     /locus_tag="ONT85_003024"
     CDS             289..2934
                     /locus_tag="ONT85_003024"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HCR2379900.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            3244..4557
                     /locus_tag="ONT85_003025"
     CDS             3244..4557
                     /locus_tag="ONT85_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HCR2379901.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            4547..5200
                     /locus_tag="ONT85_003026"
     CDS             4547..5200
                     /locus_tag="ONT85_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HCR2379902.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            5255..5806
                     /gene="radC"
                     /locus_tag="ONT85_003027"
                     /pseudo
     CDS             5255..5806
                     /gene="radC"
                     /locus_tag="ONT85_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
     gene            5937..7022
                     /locus_tag="ONT85_003028"
     CDS             5937..7022
                     /locus_tag="ONT85_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR2379903.1"
                     /translation="MKVQRIEVENKPYPLYLLLDKEYQLIEPVMKFIKYLDNTGKSPN
                     TIKAYCYHLKLLYEFMEQRGVILNDINFELLADFVGWLRYPSASNVIDLQSKKAIREE
                     TTVNTILNVVMSFLDYLSRLGEFKSIDVFKQAKGRNFKGFLHHVNKGRYQKNVLKLRV
                     KKKQIRTLRSEEVKQIIDACHTKRDKLILMLMYEGGLRIGEVLSLRLEDIVTWDNQIH
                     LTPRDVNVNEAYIKLRKERTIHVSKELMSLYTDYLIYEYSEELEHDYVFISLKEGYFG
                     KPLKYQSVLDLVRRIVKRTGIEFTSHMLRHTHATQLIREGWDVAFVQKRLGHAHVQTT
                     LNTYVHLSDQDMKNEFNKYLERKEHKK"
     gene            7019..8911
                     /locus_tag="ONT85_003029"
     CDS             7019..8911
                     /locus_tag="ONT85_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001833801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR2379904.1"
                     /translation="MNASSKRKIISQSEISKKIAVMNEEMQGFWANNSWDIRKCPHPS
                     AIELSKNPALRNRWVRFERVKNLWLRTELKYFYFYHLNNGIWNAKTVWIRKGTVINKM
                     LDFLDLKYPSITSITEVPIEKAMTEYRTYLTKRGVRITTTNYKITANQEKTPVKANSY
                     YVTNLKQFMEFYENFYFDGEEWDKDVWDRRNLPLPDDKVNPTQYEYTINFKGFRNTYF
                     KQLVKRYCKLRLNMDSFSYVSDIAQRLKEFFNFLDMKFKQVQRVHQLTRVEIEAYLSE
                     LNMMGIKPSTITGRISILEGLFSTLLRLEWDDVPSKILIYSEDYPKIPRAKPRFIDEF
                     VLEQLNSHLDKLPEYIATMTMIVQECGMRISELCTLKKGCLLEDKDGDFFLKYYQWKM
                     KKEHIVPISKEVALLIKVREDKVSEEFPDSEYLFPRKDGSPLKQETFRGELNKLAYEQ
                     NIVDKSGEIYRFHAHAFRHTVGTRMINNGMPQHIVQKFLGHESPEMTSRYAHIFDETL
                     KNEFTKFQEKLVTNNGDVLDLDEDNEVDDVELQWFKKNINAQVLPNGYCRLPVVAGGC
                     PHANACLDCTHFCTSKQFLPQHEEQLERTEELLAIAKDKQWQRQVETNSRVKERLEQI
                     IGSLTG"
     gene            8918..9295
                     /locus_tag="ONT85_003030"
     CDS             8918..9295
                     /locus_tag="ONT85_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR2379905.1"
                     /translation="MDKQVRNTTEIVRLAKQKSKKTREKVDKAISKFSIEGKVINFNS
                     IAKEANVSKSWLYKEHDIRQRIESLRERQITANVVSKPKKSSRSEEILIKTLKRRVME
                     LEKENKKLQNQIQKLYGDLYNKE"
     gene            9449..10231
                     /locus_tag="ONT85_003031"
     CDS             9449..10231
                     /locus_tag="ONT85_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(9)-Ia"
                     /protein_id="HCR2379906.1"
                     /translation="MSNLINGKIPNQAIQTLKIVKDLFGSSIVGVYLFGSAVNGGLRI
                     NSDVDVLVVVNHSLPQLTRKKLTERLMTISGKIGNTDSVRPLEVTVINRSEVVPWQYP
                     PKREFIYGEWLRGEFENGQIQEPSYDPDLAIVLAQARKNSISLFGPDSSSILVSVPLT
                     DIRRAIKDSLPELIEGIKGDERNVILTLARMWQTVTTGEITSKDVAAEWAIPLLPKEH
                     VTLLDIARKGYRGECDDKWEGLYSKVKALVKYMKNSIETSLN"
     gene            complement(10357..11088)
                     /gene="erm(A)"
                     /locus_tag="ONT85_003032"
     CDS             complement(10357..11088)
                     /gene="erm(A)"
                     /locus_tag="ONT85_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(A)"
                     /protein_id="HCR2379907.1"
                     /translation="MNQKNPKDTQNFITSKKHVKEILNHTNISKQDNVIEIGSGKGHF
                     TKELVKMSRSVTAIEIDGGLCQVTKEAVNPSENIKVIQTDILKFSFPKHINYKIYGNI
                     PYNISTDIVKRITFESQAKYSYLIVEKGFAKRLQNLQRALGLLLMVEMDIKMLKKVPP
                     LYFHPKPSVDSVLIVLERHQPLISKKDYKKYRSFVYKWVNREYRVLFTKNQFRQALKH
                     ANVTNINKLSKEQFLSIFNSYKLFH"
     gene            complement(11146..11205)
                     /locus_tag="ONT85_003033"
     CDS             complement(11146..11205)
                     /locus_tag="ONT85_003033"
                     /inference="COORDINATES: protein motif:HMM:NF033690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythromycin resistance leader peptide"
                     /protein_id="HCR2379908.1"
                     /translation="MGTFSIFVINKVRYQPNQN"
     gene            11597..12259
                     /locus_tag="ONT85_003034"
     CDS             11597..12259
                     /locus_tag="ONT85_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HCR2379909.1"
                     /translation="MNTIESQFDKVAEDYDFVNELLNDYSFFVSNMSPKKGRALDIGC
                     GSGLLVEKLASYYDEVVGIDISNQMLDLAKSKRQLTNTVYLNMNAEQLNFNEKFDFIV
                     SRTTFHHLDDIASVIQQMKELLNEEGRIVILDNVSEVETPPTYVYKLGAIQEFLPHCF
                     KFGIKNAIRIYNHNTSKSWLEHLASDKYLSEQNYYDLYEKLLPGCQFHKMGWAMGVVW
                     TK"
     gene            12494..12637
                     /locus_tag="ONT85_003035"
                     /pseudo
     CDS             12494..12637
                     /locus_tag="ONT85_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein"
     gene            12787..12987
                     /locus_tag="ONT85_003036"
     CDS             12787..12987
                     /locus_tag="ONT85_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HCR2379910.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(13241..14920)
                     /locus_tag="ONT85_003037"
     CDS             complement(13241..14920)
                     /locus_tag="ONT85_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HCR2379911.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(14922..15134)
                     /locus_tag="ONT85_003038"
     CDS             complement(14922..15134)
                     /locus_tag="ONT85_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HCR2379912.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            15527..17257
                     /gene="efrA"
                     /locus_tag="ONT85_003039"
     CDS             15527..17257
                     /gene="efrA"
                     /locus_tag="ONT85_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HCR2379913.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            17254..19014
                     /gene="efrB"
                     /locus_tag="ONT85_003040"
     CDS             17254..19014
                     /gene="efrB"
                     /locus_tag="ONT85_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HCR2379914.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
BASE COUNT         6277 a         3053 c         3963 g         5726 t
ORIGIN      
        1 aaaaaaaaaa ccattgtaga atgaagcata acgctgaaca agaggagtaa ctggcacatt
       61 cattcttaga gagagaacta tctgctgaaa ggttctcaat gacaaccagt gaaggtagct
      121 tgggagtttc tgtcttgaat ggagtagaga tagacgagtc gtgatcgtta actcacttga
      181 gaggcaataa tgattgatta ttgcaattta ggtggtaccg cgtaaatcat acgtcctaga
      241 atacgaaagt attttaggac gttttttagt ttaaaggagg aaaacagcat gacggaagaa
      301 aataacctat caacaaaata taatccgcaa gaagttgaag ctggacgtta tcagcattgg
      361 ttagaacaag atttgtttaa accgagcgga gataaaaaag caaaacctta ttcaatcgta
      421 atcccgcccc caaatgttac tggaaaactt catttagggc atgcttggga tacgaccttg
      481 caagatatgc tgattcgtca aaagagaatg caaggatatg atactttatg gctgcctggt
      541 atggaccatg caggaatcgc gacacaagca aaagtagaag cgaagcttag agagcaaggg
      601 atttctcgtt atgatctagg tcgtgaaaag ttcatcgatc aagtgtggga atggaaagaa
      661 gagtatgcga gccacatccg cgaacagtgg gctaaaatgg gcttatcttt agattacagt
      721 agggaacgtt ttacattaga tgatggatta tctgaagcag tacgtaaagt ttttgttacc
      781 ttatacgaaa aaggtttgat ttatcgtggt gaatatatca tcaactggga tcctcaagca
      841 cgaacagctc tatctgatat cgaagttatt cataaagata tcgaaggcgc cttttatcat
      901 atgagctatg aactagcaga tggatcaggc gtagtagaaa ttgcgacaac ccgtccagaa
      961 acgatgttgg gagatacggc gattgctgtt catccagaag acgagcgtta tcaagcgctc
     1021 atcggcaaaa aagtgattct acctttagtc aataaacaaa tcccaatcat tgcagatgaa
     1081 tatgtagata tggaattcgg gacaggtgta gtaaaaatca ctccagcaca tgatccaaac
     1141 gactttgagg taggtaatcg tcatgatctt ccacgcgtaa atgttatgga tgatgatggt
     1201 acgatgaatg aactagcagg aaaatatgct ggaatggaac gttttgctgc tcgtaaagca
     1261 atcgtgcaag atctgaaaga aatcggtcga ttgattaaaa tcgatacgat gatccacagt
     1321 gtgggtcatt ccgagcggac aggtgtcgtt gttgaacctc ggctgtctac tcaatggttt
     1381 gtaaaaatgg cgccgttagc gaaaaaagcc attgataacc aagaaacaga tcaagcagtg
     1441 gaattcttcc ctccaagatt caatcaaacg tttttacgtt ggatggaaaa tgtccatgat
     1501 tgggtaatct cacgacaatt atggtgggga catcaaattc ctgcatggta tcacaaagaa
     1561 acaggtgaaa tgtatgtagg gatggaagca ccagcggata gtgaaaactg ggtacaagat
     1621 cctgatgttt tagacacttg gtttagttca gctttgtggc ctttctcaac aatgggctgg
     1681 ccagatacgg aaagcgaaga ttataagcga tacttcccta ctagcacttt agtaaccggt
     1741 tatgacatca ttttcttctg ggtaagcaga atgattttcc aaagcttgga atttactggc
     1801 gaacgtccat ttgagaatgt cttgattcat ggattgattc gtgacgaaca agggcggaag
     1861 atgagtaaat cattaggtaa tggaatcgat ccgatggaag taatcgaaaa gtatggagca
     1921 gatgctctcc gctggttctt atcgaatggt tctgcaccag gacaagatgt ccgcttcagc
     1981 tatgaaaaaa tggatgcttc ttggaacttt atcaacaaaa tctggaatgc ttcacgtttc
     2041 gttatcatga atatcgaagg aatgaccgta gaagagatcg atttatctgg agaaaaatca
     2101 gtggctgacc ggtggatcct tactcgtttg aatgaaacga tcgaacgagt aactgagcta
     2161 tttgatcgtt ttgaatttgg tgaagcaggt cgacaactgt ataactttat ctgggatgat
     2221 ttttgtgact ggtacatcga aatgagtaaa gaagtacttt atggagatga tgctgtttca
     2281 aaacaaacca cccgtagcat cttagtccat acacttgatc agatcctacg tttattgcat
     2341 ccaatcatgc cattcgttac agaagaaata tgggaacata ttcctcatca gggaaattct
     2401 ctagtagtgg cagaatatcc tgttgtacgt ccagagtttg atgatgaaac agcttctaaa
     2461 gggatggaag tattgaaaga attgattcga gccgttcgaa atattcgttc agaagtgaat
     2521 acaccattgt ctaaaccaat tacgttgcta attaaaacaa acgatgctaa aatcgatcaa
     2581 tttttagttg aaaatacaag ctatatcgaa cgcttctgta atcccgaaga gttgacgata
     2641 tcaagtgaga tcactgcgcc agatctagcg atgtcagctg ttctgacagg tgcagaaatc
     2701 ttcttgccgt tagcaggatt gatcaatatt gaagaagaaa tcaaacgatt agaaaaagaa
     2761 ctggaaaagt ggacaagcga agtaaaacgt gtccaaggaa aactttctaa tgaacgtttt
     2821 gtttctaacg cgccggaaga agtagtagag gcagagcgtg caaaagaaaa agattattta
     2881 gagaagcaaa cggctgtcaa agaacgaatc gaaagtttgc gtactattca ataagaatag
     2941 aaaaagagag tgtgaaatca gctagctatt actaaaagct agctgattga acatcgttac
     3001 aggaaagagt ttgagcctca ctcttagctt cgattcataa ggagatgtga cataaatttt
     3061 gtcgcatctc ttttttagtc tactcttatt atgtaggaga aggagggagg tggatcatgt
     3121 atcaagagtc aataaagtag ttgataaaat cgttcctctc tctgtttctt ttctctttgt
     3181 catactttta tttttcttat gtgttctatt tgttataata aaagagaaat gggggaattg
     3241 ttgatgacga ttgaagaagc aatagaatgg atccatagtc gattaccatt tggttcacgt
     3301 cctggccttg accgagtcga agctttatta gaagtggtag ggcatccaga gagaaaagta
     3361 aaaacgatcc atatcgctgg aacaaacgga aaaggttcta ctgtagctta tttgagaagc
     3421 ctattagaag aggctgggct gacagtaggt acatttactt caccttatat cgaaacgttc
     3481 aatgaacgga tcgcaatcaa tggcaaaagc attccagaca atgatttgat ccagcttgta
     3541 aaaaaatatc agccactgat caaacaactt gatcaatttg aagcagtagc tggtattact
     3601 gaattcgaga cattaacagc aatggcattt gattattttt tgatgaagaa agtggacgta
     3661 gcgattatcg aagttggctt aggcggctta ttggattcta ccaatgtagc tgtaccaatg
     3721 ctgacgggaa taacaacgat tggacttgat cataccgaaa tacttggaga tacaattgaa
     3781 gaaatcgctg cacaaaaagc tggaatcatc aaacaaggcg ttccagtagt aacaggaaat
     3841 attgccccag aagcttttac tgtcatccaa caaacagcta aagcaaaaaa tgcaccgctt
     3901 tatgtatacg gggaaaatta taaaataaac tatctgcatc ctgatgagat gtggggagaa
     3961 gtatttgaat tttttaatga aataggaaag atccctcaat taaccactcc gcttttaggg
     4021 caacatcaag ctgaaaatgc aggagtagcc atcgaattgt attttgttta ctgtcaattg
     4081 gaaaagctgc ctttccgaga aaaagatgtt ttgaagggat tgaagaaatc tcgatggcca
     4141 ggaagaatgg agcgtttgag taacgagcca ttagttgttt tagatggtgc ccataatacg
     4201 catgcaatga accggcttgt tgagaatttg aaaaaagaat tcaaaggata tcaaatcaac
     4261 attttgtttt ccgccttacg aactaaaaat gttagagaaa tgctagaacg attgcagcaa
     4321 gtaccaaatg tccatattta tttgacgacc tttgaacatc caaaagccat cgaattaagt
     4381 gaagtatcag atttagcaag tgagagagta tcaatcgttt cattgtggca attcggatta
     4441 gcagatatat tggaaaagat gacaaatgaa gacgtattag ttgtgaccgg ttcactttat
     4501 ttcatttcag aagttcgcca tttattaata caattaggag gagatcatga agtataatgc
     4561 agtgattttt gacatggatg gtttgctgtt cgacacagag ttagtgtatt acgaagccac
     4621 acaagtagta gcagacaaga tgggttttcc ttatgataaa gacctgtatt taaaattctt
     4681 aggtgtttca gatgaagaag tatgggcgaa ttatcatatg attttctcgg aatttggcaa
     4741 aacaagtgta caaagattca tagatgattc ataccaggaa acgcttgaac gtttttcgca
     4801 aggagcggta caaatgaagc ccggtgtgtt ggaactcctc tcttttttag aagaacatca
     4861 gattcccaaa gttgtcgctt ccagtaacca acgagcagtc attgaacttt tattgaagaa
     4921 gaacaatctg tttgaacgat ttgagacgat tgtttctgca gaaaatgttc aacgagcaaa
     4981 accagaccca gaaattttct tgttagctaa tcagaagcta ggtacagcga agaaagaaac
     5041 attgatttta gaagattcac aaaatggtat tttagcagct tctgcggctg agatcccggt
     5101 agtcatggta ccagatctgc ttccaccatc ccaagaattg atcaaaaaaa cagctggggt
     5161 tgtttcttct ttacatgaaa tccctgcatt gttaaaataa aaatccatct cttttcgtat
     5221 cttattttag ataagataga aaggagatgt ttttttggga aaacgattga tcaaagaagt
     5281 acctactagc tctttaccaa gggaacgtat ggaaatttat ggcgcggaag ctttatcgga
     5341 tcaggagctt ttggcaatct tactgcgtac cggtcaacac ccttatagtg tgatgtcgat
     5401 tgctggaaat ctgttaaaaa cgtttggagg actggcctct ttacgacagg ctactttgca
     5461 tgagttagaa gagattcgag ggataggacg cgtgaaagca atcgaaatca aagccttgat
     5521 cgaattaggt cgacgaattc agacggatca tgaaagagcg gctccaccgg ttcgtaccag
     5581 ctacgaattg gcccagcaat tgattctgga aatgaaagat cacaaacaag aacatttggt
     5641 ttgtatttat ttggacacga agaatcaagt gatcttaaaa aaaacagtgt tcatcggttc
     5701 gctgaatcag tcagtcgctc atcctagaga aattttccat tatgctgtac gttattgtgc
     5761 agcaaggatc gttttagcgc ataatcatcc ttctggtaat gttttaacca tagtataata
     5821 aataatagta gttaaagtag tttaagagga gatattaaag ggatatttta ctttaaataa
     5881 aaattaacaa ggtttataat ttgtagtagt taaagtattt aaagaggtgg gaacatgtga
     5941 aggttcaaag gatagaagtg gagaataagc cgtatccatt gtatttatta ctagataaag
     6001 aataccagct aatagaacca gtaatgaaat ttattaaata cttagataat actggtaagt
     6061 ctcctaatac cattaaagca tactgctatc atttaaagtt gctgtacgag ttcatggaac
     6121 agagaggtgt tattcttaat gatattaact ttgagttgtt agcagacttc gtaggttggt
     6181 tgagatatcc ttcagcatca aatgtaattg atcttcagtc aaaaaaagcc ataagagaag
     6241 aaacgacagt gaatacaatt ttaaatgtag ttatgagttt tcttgattat ttaagtagat
     6301 taggagaatt taaatcaatt gatgtattta aacaagcaaa gggaagaaat ttcaaaggat
     6361 ttttacatca tgttaataag ggtagatacc aaaagaatgt cttaaagtta agggttaaaa
     6421 agaaacagat aagaacattg agatcagagg aagttaagca aattattgat gcttgtcata
     6481 cgaaaagaga taaattaatt ttaatgctta tgtatgaggg tggtttaaga atcggtgaag
     6541 tgttatcgct taggcttgaa gatattgtca cttgggacaa tcaaatccat ttaacaccta
     6601 gagatgttaa tgttaatgaa gcttatatta aattaaggaa ggaaagaaca atacatgtga
     6661 gtaaagaact tatgtcactt tatacagatt acttgatata tgagtatagt gaggaattgg
     6721 agcatgatta tgtttttatt tccttaaaag aaggctattt tgggaaacca ctaaagtacc
     6781 aaagtgttct tgatctagtt agaagaatag ttaaaaggac tggaatagaa tttacatcac
     6841 atatgcttcg ccacactcac gcaacgcagc taattaggga aggatgggat gttgcgttcg
     6901 ttcaaaagag attaggtcac gcacatgttc agacaacgtt aaatacctat gttcatcttt
     6961 cagatcagga tatgaaaaat gagtttaata aatacctcga gagaaaggag cataagaaat
     7021 gaatgcttct agtaaaagga aaattattag tcagagtgag attagcaaaa aaatagctgt
     7081 aatgaatgaa gaaatgcagg ggttttgggc taataatagt tgggatataa gaaaatgtcc
     7141 acatccttct gccatagaat taagtaagaa tcctgcttta aggaatcgtt gggttcgttt
     7201 tgaacgtgtt aaaaatctgt ggttaagaac agaattgaaa tatttttatt tttaccattt
     7261 aaacaatgga atatggaatg caaaaactgt ctggattaga aaaggaacag taattaataa
     7321 aatgttagat ttcttagatt taaagtatcc tagcattact tcaattactg aagttcctat
     7381 tgaaaaagca atgacggagt atagaactta tttgacaaaa cggggtgtta gaattaccac
     7441 tactaattat aagattactg ctaatcaaga aaaaacacct gtaaaagcta attcctacta
     7501 tgttactaat ctaaaacaat ttatggagtt ttatgagaac ttttattttg atggagagga
     7561 gtgggataaa gacgtttggg atagacgtaa cttacctttg ccagatgata aggttaaccc
     7621 aacacaatat gaatatacaa ttaactttaa agggtttcgg aatacatatt ttaaacaact
     7681 tgtaaaaaga tattgtaagt tgagattgaa catggatagc ttttcctatg taagtgatat
     7741 tgcccaaaga cttaaagagt tctttaattt tctggacatg aaatttaaac aagttcagag
     7801 agtacaccaa ttaacgagag tggaaattga agcatattta agtgaactaa acatgatggg
     7861 aataaaacct agtacaataa ctgggaggat ctctatattg gaaggactat ttagtaccct
     7921 tcttaggcta gaatgggatg atgttccttc caaaatatta atttattctg aggactatcc
     7981 gaaaatacca agagcaaaac cacgctttat agatgaattc gtcctagagc aattgaacag
     8041 tcatcttgat aaattacccg aatatatagc tacgatgact atgattgttc aagaatgtgg
     8101 aatgaggata agtgaattgt gcaccttgaa aaaaggctgt ctattagagg acaaagatgg
     8161 agatttcttt ttaaagtatt atcaatggaa aatgaaaaag gagcatatag ttccaatatc
     8221 taaagaggta gctttactta ttaaagttcg ggaagataaa gtttcagagg aatttccaga
     8281 tagtgaatac ctctttccaa gaaaagatgg atcgccatta aaacaagaaa catttagagg
     8341 tgagttaaat aaattagctt atgagcaaaa tatagtggat aaatcaggtg agatttatag
     8401 attccatgcc catgcctttc gccatacagt aggaacaaga atgattaaca acgggatgcc
     8461 ccagcatatt gtgcagaaat ttttggggca tgaaagccca gaaatgacaa gcagatacgc
     8521 tcatatcttt gatgaaactc taaaaaatga atttactaaa tttcaggaaa aactggttac
     8581 caataatgga gatgtgcttg atctagatga agataatgaa gtcgatgatg tagagcttca
     8641 atggttcaag aaaaatataa atgcacaagt gcttccaaat ggttattgta gattgccagt
     8701 agtagcaggt ggttgtccac atgcgaatgc atgcttagat tgcactcact tctgtaccag
     8761 taagcaattc ttaccacagc acgaagaaca gttagagcgt acagaagagt tattagccat
     8821 agctaaggat aaacaatggc aaagacaagt agagactaat agccgtgtta aagagcgttt
     8881 agaacaaatc attggaagtt tgacggggta attatcaatg gataaacaag ttagaaatac
     8941 aacagaaatt gtacgtttgg cgaagcagaa atcaaaaaag acaagggaaa aagtagacaa
     9001 agcgatttct aaattttcga ttgaaggtaa agttattaat tttaattcaa tagcaaagga
     9061 agctaatgtt tctaaatcat ggctttataa ggaacacgat attaggcaaa gaatcgaatc
     9121 ccttcgtgag cgtcaaataa cagcaaatgt agtctcaaaa cccaagaaaa gttctcgttc
     9181 ggaggaaatc cttattaaaa ccttaaaaag aagagtaatg gaattagaaa aagaaaataa
     9241 aaaattacag aaccaaattc aaaaattata tggagatctg tataataaag aataattatt
     9301 aatctgtaga caaattgtga aaggatgtac ttaaacgcta acggtcagct ttattgaaca
     9361 gtcatttagg tatatgtcca atctagggta agtaaattga gtatcaatat aaactttata
     9421 tgaacataat cagttacgag gtgaaatcat gagcaatttg attaacggaa aaataccaaa
     9481 tcaagcgatt caaacattaa aaatcgtaaa agatttattt ggaagttcaa tagttggagt
     9541 atatctattt ggttcagcag taaatggtgg tttacgcatt aacagcgatg tagatgttct
     9601 agtcgtcgtg aatcatagtt tacctcaatt aactcgaaaa aaactaacag aaagactaat
     9661 gactatatca ggaaagattg gaaatacgga ttctgttaga ccacttgaag ttacggttat
     9721 aaataggagt gaagttgtcc cttggcaata tcctccaaaa agagaattta tatacggtga
     9781 gtggctcagg ggtgaatttg agaatggaca aattcaggaa ccaagctatg atcctgattt
     9841 ggctattgtt ttagcacaag caagaaagaa tagtatttct ctatttggtc ctgattcttc
     9901 aagtatactt gtctccgtac ctttgacaga tattcgaaga gcaattaagg attctttgcc
     9961 agaactaatt gaggggataa aaggtgatga gcgtaatgta attttaaccc tagctcgaat
    10021 gtggcaaaca gtgactactg gtgaaattac ctcgaaagat gtcgctgcag aatgggctat
    10081 acctctttta cctaaagagc atgtaacttt actggatata gctagaaaag gctatcgggg
    10141 agagtgtgat gataagtggg aaggactata ttcaaaggtg aaagcactcg ttaagtatat
    10201 gaaaaattct atagaaactt ctctcaatta ggctaatttt attgcaataa caggtgctta
    10261 cttttaaaac tactgattta ttgataaata ttgaacaatt tttgggaaga ataaagcgtc
    10321 ctcttgtgaa attagagaac gctttattac tttaatttag tgaaacaatt tgtaactatt
    10381 gaaaatagaa agaaattgtt ccttcgatag tttattaata ttagtgacat ttgcatgctt
    10441 caaagcctgt cggaattggt ttttagtgaa aagaacacga tattcacggt ttacccactt
    10501 ataaacaaaa gatcgatact ttttgtagtc cttctttgaa atcaatggtt gatgtcgttc
    10561 aagaacaatc aatacagagt ctacacttgg cttaggatga aaatatagtg gtggtacttt
    10621 tttgagcatt tttatatcca tctccaccat taatagtaaa cccaaagctc gttgcagatt
    10681 ttgcaatctt ttcgcaaatc ccttctcaac gataagatag ctatatttag cctgactttc
    10741 aaaggtaatt cttttgacaa tatccgtact gatgttataa ggaatattac catatatctt
    10801 atagtttata tgttttggga aggaaaattt tagaatatcc gtttgaatca cttttatatt
    10861 ctcagagggg tttaccgctt ctttagtcac ttgacataag cctccatcaa tttctatagc
    10921 agtaactgat cgactcattt tgactagctc tttggtaaaa tgtccttttc ctgatccgat
    10981 ttctattacg ttgtcttgtt tactgatatt cgtgtgattc aatatttctt ttacatgctt
    11041 tttagaagta ataaaatttt gcgtgtcttt agggtttttc tggttcatta taaccttctc
    11101 cttactggtt ataatgaact gacattaagc gttcattata accaattaat tttgatttgg
    11161 ttgataacga actttattaa taacaaatat agaaaaagta cccatacttt tatctccttt
    11221 ccattttttt cattatgaat gagataaagt aatttctact actgcgatac tggtgcacat
    11281 aattgaaacc tccttatgta actgaatatg attgtatcat cttttcattt tagaataaac
    11341 ctttatatgt aattttgcac ttgacacttc agatggacgt tttcttcaaa ataatatgtt
    11401 ttgaaacttg ataagagaag ccactgtctt tactaaaaag taaaggctgc gataaaggac
    11461 agtgacttta ataaagttgc actgttgtat tgtctttgga taaaattgtc taaagggaag
    11521 ttttatcccc aacgaaaaaa catctaaagt ggtaagtaat gaaagaatgt cgataaagta
    11581 ggaatggggg atctttttga atacaataga atcacagttt gataaggttg cagaagatta
    11641 cgatttcgtg aatgagcttt tgaatgatta ttcattcttt gtgtctaata tgtctccaaa
    11701 gaaaggcaga gcattagata tcggatgtgg ctcgggtttg ttagtggaga aattagcaag
    11761 ttattatgat gaagtggtag ggattgatat ttctaatcaa atgctcgatc ttgccaaatc
    11821 taaacgtcaa ctaacaaata cggtctatct gaatatgaat gcagaacaac ttaattttaa
    11881 tgagaagttt gattttattg taagccgaac aacctttcat catttggatg atatagccag
    11941 cgtaatacag caaatgaagg aactgttgaa tgaggaagga agaatagtta ttcttgataa
    12001 tgtatctgaa gtcgagacac cgcctaccta tgtttataaa ttaggggcaa tccaagagtt
    12061 tttaccgcat tgtttcaaat ttggaataaa aaatgcgatt agaatttata atcacaacac
    12121 atctaaatca tggcttgagc atttagcttc agataagtac ctatctgaac aaaattatta
    12181 cgatttatat gaaaagttgt tgcctggatg ccaattccat aaaatgggtt gggcaatggg
    12241 ggttgtctgg acaaaataac ttaaagctgt ggctttgaaa agttgaattt gtgattgttt
    12301 tcagagctat cgggtgcttt agtataaata acaatgaata aaaaatcagc ctgtatctta
    12361 tgttactatt aagtaattta agatacaggt tttactattt ttgaaataag taccttccac
    12421 ttcaaataca tgtattcaaa gtgaaagtat tgatttaaat gattcttttg taatttactc
    12481 ttgataatat gaaattccat cgcagcagga tatgaatttt accaaaagga tacaaaagtg
    12541 tggagaaatg atggggatca ctgtactgga ccacttgatc atcggacgca aacgatattt
    12601 tagtttgaga gaagaaggaa tgatggaaga gaagtaagtt gctttcttgc aaaaaaaatg
    12661 aagcaagtgt acaattatat tgtaattttg tttgggaata gaagttgaaa catctgttgc
    12721 acctctatcg tacacgagca atgttacaat ttaaattagt gcttttggca cgaggaggat
    12781 attattatgg aaaacggaac agtaaaatgg ttcaacgctg aaaaaggatt tggatttatc
    12841 tcacgtgaag atggaagcga cgtattcgtt cacttctcag ctatccaagg tgacggattc
    12901 aaaacattag aagaaggtca agcagtgact ttcgacgttg aagaatcaga ccgcggccca
    12961 caagctgcaa acgttgtaaa agcttaattt tatctgtaaa tcaaattgat ggtagacatc
    13021 aatttttacg agagaacaaa gcgagtaaca aaaatacctg ccacaagaga caatttctct
    13081 tgtggcaggt atttttttgt atgaaaaagt aaataaattt aagaggctat gaaagagcca
    13141 ccttgtatca aggaataaga acaaacaaaa atggcttctc atgtttttca tttgttttct
    13201 cttatattca ggacaaggtg ctgatttcat aacctcttat ttattcttca tctggtgtca
    13261 ttaccattgg caaaatcatt ggatgtcttt ctgtacgttc gaataagaaa ggctgaagcg
    13321 cgccagtcat cgcttctttc aatttatatt ctgaacagtc aggtccattc aaacattctc
    13381 ttaaagcgtg gaataagatt cgctgagctt cattgatcat ttctcctgat tctcgcatgt
    13441 agacaaatcc acgagagaga atatctggac cagccaagat ttcttttttc ttgatatcca
    13501 ctgtcgcgac agctaataca agaccttctt ctgaaagcag gtgacggtca cgtaaaacga
    13561 cattaccaat atcaccgatc ccgctaccat cgatatacac atctccagcc acaaaatgcc
    13621 cagcaggacg cgcagaatcg ctagttaacg ctaaaacatc gccattttcc atgatgaagc
    13681 agttttctgc aggaacacct acatcttgtg ctagagaagc atgtactttc aacatgcgga
    13741 attctccgtg gacaggaata aaatattttg gcttcatcaa acgaagcatt aatttttgtt
    13801 cttcttgtcc accgtgaccg gaagtatgga tgttattgat ttttccgtga atcacttctg
    13861 caccagcttc agacagcaaa ttgatcaaat gattgacact tgttgtatta ccaggaatag
    13921 gtgaacttga aaagatcact gtatcgtctg gctggatttg gatttgacgg tgagtaccat
    13981 tcgcgatgcg gctcaacgcg gccatcgctt ctccttgaga acctgtacat aggatcatcg
    14041 tttcattggc aggcaagcga ttgatttcgt gtgcatctac aaatgtgccg tctggaactt
    14101 tgatatatcc taaacgttga ccgttgacga tggcattttc catgcttcgt ccgaaaacag
    14161 cgattttacg accagttgcc acagcggcat ctgctgcttg ctgcaaacgg aagatgttag
    14221 aagcaaagct ggcaaaaatg atacgtcctt cgatttttga aaagatcttc aaaatagaat
    14281 gcccaatcgt tttttctgat ttagtaaatg ttggaatctc agcatttgta ctatcagata
    14341 aaaggcacaa gactccttct tccccgatac gagccatttt atgcaagttt gctggttcgc
    14401 caacaggagt gaaatcgaat ttgaagtctc cagtcgctac gatatttcca gaaggtgttt
    14461 tcaccacgat ccctaatgca tctgggatac tatgggtcgt tctgaaaaag ctgacagaag
    14521 tctttctaaa acggatgatc gtatcttcat tgatctcatg gagctctgca tctcgaagca
    14581 agccatgctc atctaatttg tttgtgatca acgctaatgc gagaggacca gcatagatag
    14641 gaatatttgc ttgtttcagc aagtagggta ttccgccgat gtggtcttcg tgcccgtgag
    14701 taatcaccaa ggcttttact ttgtgtaagt tttgtatgat gtagctgtag tcgggaatga
    14761 catagtcaat tccaagtaaa tcgtcttccg ggaatttgat cccggcatca atcaaaatga
    14821 tttcatcttg gaattgtacc ccatacgtat ttttcccgat ttcacctaat ccaccaatag
    14881 caaagacgcc tgtctcgtta tttttgatgg aaagtttcat attaaaactc cactaatgag
    14941 aaatcagcat tttctttttc gtactctaga tgacttcctt ctagaagttg aacgaattca
    15001 atattgtatg gggtattttc ttccacacgt tggcgagcgt ctacatcgct ttttgcctca
    15061 atgtaaagag attttgtttc ttcccgttta gggttgcgta cttttgtttc ttgataataa
    15121 actttataga tcattgtttt gtctccttta tctgcgtaaa gcagtgattt agctagttga
    15181 tttttttaga caacacaaaa cgaggggaca agccacctgg tttttcatat gctttgacta
    15241 gaatactgga ttcgagtcaa agagacaagc ctcgtttttg cttatctgtt cggatatttt
    15301 ttcaaccgta ccttctgtat aaaaaatata caacaattaa ttgttattct agcataaaac
    15361 agcaaataag tatagaaatc aactagattg aaagaaaaaa tcaatgttta tcatcccgct
    15421 ataatcataa tgactttttt atgagagaag tgttctatta ataggctcgt tgtgctatca
    15481 tttttagtaa tgaaaatgta acaaaagatt aaaaggagat gagaaagtga aactaatgtg
    15541 gcattatacc atgcgttata agaaattctt gtttttcaat ttcatatgcg tatttggttt
    15601 tattttgatt gaattaggac ttcctacgat tttagcacgt atgatcgatg taggaatccg
    15661 caatgatgat tttgattatg tgaagcaaca agggatattt atgatcgtta ttactgtaat
    15721 cggtattatt ttgaatatct tgttaggtta ttttggttcg aggattacga ctaatatcgt
    15781 agcagatatt cgagatgatt tgttcgaaaa gatccaaagt tattcgcatc aggaatacga
    15841 aaatctaggc gtatcttcat tgatcactcg tacaacaaac gatgcttatc agatcatgtt
    15901 gttcctgcaa aatattttac gtatcggttt tatggcacca atgatgttca ttgtcagttt
    15961 gtacatggtc atgcgtacaa gtgtgacact cagtttgtat gtagtaggtg cattaccttt
    16021 actattattg gcagtcgtag cgattgctaa ggtgtctgaa ccgttatcta aaaagcaaca
    16081 aaaaaactta gataaaatca acagtatctt gcgggaaaat ctttcaggat tgcgtgtgat
    16141 tcgtgcattc gtcaatgaaa agtttgaaga aaaacgtttt gctgtagtca atgagaatta
    16201 tgcttctagt tcaaaaagct tattccgttt gatggctgtt gcacaaccgg gtttttattt
    16261 cttatttaat atcgtaatgg tattgatcat ctggagcggg gcagtgcaaa tcgatgcagg
    16321 cgagcttcgt gtaggagatt tgattgcgtt tatcgagtat atattccatg ccttgttctc
    16381 attcatgtta tttgctagtg tgttcatgat gtatccaaga gcagctgttt cagctggacg
    16441 tatccaagaa gccctagatg ccaaaccttc gattgtagaa gatccaaatg gtatatctga
    16501 tacaaaaact aaagggtata tcgaatttca aaatgtaaca tttgcttatc caggccattc
    16561 gcaagagcca gtgatacgaa atgtcagttt tacagcctct ccaggagaga ccgttgcttt
    16621 tattggaagt accggtagcg gaaaatctac gttgatccaa ttgatccctc gcttttatga
    16681 tgtgagtgaa ggaaagatct tgatcgatgg agtagacgtg agagattatc ggctaagcaa
    16741 gttgcgagat aagattgggt ttattccgca aaaagcgctg ctatttactg gaacgattgc
    16801 agataatttg cgatacggga aagaagatgc tacgcaagaa gaaatggaac gtgcagctga
    16861 gattgctcaa gcttcagaat tcatttctcg aaaaccagat ggatttaatg aacatttatc
    16921 agaaggtggt tccaattttt caggtggaca aaaacaacga ttggcaatag ccagagccat
    16981 catacgccgt ccggaaatct atatttttga tgacagcttt tctgcattag attatcaaac
    17041 ggatgcgaag ttacgtgctc gtctgaaaaa agaaacgaca gaatctactg tattgatcgt
    17101 agcacaacgt gtgggaacga tcatgcatgc agataagatc atcgtattaa atgaaggaga
    17161 agtcgtcggt atggggacgc atcgggagtt gttggaaaaa tgcccgattt attatgatat
    17221 tgccgcttct caattatcaa aggaggaatt agcatgaaaa aaaccttttc ttccttaaaa
    17281 cgtttagctc gatatatcag accgtatcgt gagacttttc ttttagtgat cctatttaca
    17341 gttctgacag tggctttcaa cgtggctttg ccatatgtta caggacttcc tactacagaa
    17401 atcagccgta atattgccaa tggcgaatca atcaattttt cttatatatt caaatgtctc
    17461 atttggatca cagttgttgg aatagggtat tgtatttcgc aattactttc tggtgttttg
    17521 atgacaaatg tcgttcaaag tgccatgcat gatttgagaa gagacataga agaaaaaatc
    17581 aatcgtcttc cggtttcata tttcgataaa aaccagcaag gaaatatttt gtctcgtgtg
    17641 acgaatgatg tagatgcagt cagcggtgct ttacaacaag cttttattgg tatcgtaaat
    17701 gcgatattgg gaattacgtt ggctgcagca atgatgtttt atatccagcc gatgatggcg
    17761 ctgatctcta tgatcatgat tccaacatcg atctggatct caaaacgagt gatcaatgct
    17821 tcgcagaaat attttcaaag catgcagaat tctcttggtg agctaaacgg ctacgtccaa
    17881 gaaaatatga caggttttag tgtattgaaa gtatatggac gcgaaaaaga aacattggaa
    17941 ggattcaaaa aagttaatca cagcttgaaa tactttgggt ttcgtgcagc attcatttct
    18001 ggcttgatga tgcctctagt tcaattaact gcctatgcta cttatattgg tatggctgtt
    18061 ctaggaagct tttatgttat tactggggcg atcgttgtcg gtcaactaca agcattcatc
    18121 caatatattt ggcaaatcag tcagccaatg gggaacgtga cacaattgtc ttcagttttg
    18181 caaagtgcat cagcggcaac gaaacgtgta ttcgagattt tagatgagcc agaagaaaaa
    18241 gaaaacaaag tagatgcttc actgccagaa acgattttag gagatgtgac attcgatcat
    18301 gtcgattttg catatgatcc gaagaaacca ttaatccaag atttgaattt tgaagtgaaa
    18361 gctggtcaaa ccgtagctgt ggtcggtccg accggtgccg gcaagacgac attgatcaat
    18421 ctattgatgc gtttttatga cgtagacaaa ggagcaataa ggattgatgg aatcgacacc
    18481 aaatcaatga gtcgcagtga tgtgcgttcg ctatttggta tggtcttgca agatgcatgg
    18541 ctttatcaag gaacgattgc agataatatc cgcttcggaa agcttgatgc gacagattat
    18601 gaagtagttg atgctgcgaa aacagcaaac gtagatcatt ttatccgaac gatgccagat
    18661 ggatatgata tggccatcaa ttctgaggga gacaatgtgt ctttaggaca aaaacagttg
    18721 ctgacgattg cccgagcagt ggtctccgat cctaaaatct tgattttaga tgaagcaaca
    18781 agttcagtag acacccgttt agaagcgttg attcaaaaag caatggatcg cgtgatggag
    18841 ggacggacaa gtttcgtcat cgctcaccgt ttatcgacaa tccgtgatgc agatctgatt
    18901 ctagtgatgg atcaaggtca gatcattgaa aaaggaacgc atgagagttt attggcacaa
    18961 ggtggatttt atgaaaaact gtataacagc caatttgctg aggaagcaga ataagtcat
//
LOCUS       DAJSBL010000214        18888 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.guided.053, whole genome shotgun sequence.
ACCESSION   DAJSBL010000214 DAJSBL010000000
VERSION     DAJSBL010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18888
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.guided.053"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            307..1203
                     /locus_tag="ONT85_003041"
     CDS             307..1203
                     /locus_tag="ONT85_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HCR2379915.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(1353..2294)
                     /locus_tag="ONT85_003042"
     CDS             complement(1353..2294)
                     /locus_tag="ONT85_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HCR2379916.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            2420..3541
                     /locus_tag="ONT85_003043"
     CDS             2420..3541
                     /locus_tag="ONT85_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HCR2379917.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            3799..5301
                     /gene="eat(A)"
                     /locus_tag="ONT85_003044"
     CDS             3799..5301
                     /gene="eat(A)"
                     /locus_tag="ONT85_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HCR2379918.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            5329..5895
                     /locus_tag="ONT85_003045"
     CDS             5329..5895
                     /locus_tag="ONT85_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HCR2379919.1"
                     /translation="MVKGNFANEGENHMLYVTRHGETTWNAQGLVCGRADVPLTEKGQ
                     MQAQKLAEKVVDLPVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYD
                     GLPSKDENFQKARLAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIR
                     VINAYFHPMPNEGFFTFMVDNTELVSYE"
     gene            6106..7167
                     /locus_tag="ONT85_003046"
     CDS             6106..7167
                     /locus_tag="ONT85_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HCR2379920.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            7170..8066
                     /gene="murQ"
                     /locus_tag="ONT85_003047"
     CDS             7170..8066
                     /gene="murQ"
                     /locus_tag="ONT85_003047"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HCR2379921.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            8080..10062
                     /locus_tag="ONT85_003048"
     CDS             8080..10062
                     /locus_tag="ONT85_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCR2379922.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(10127..10573)
                     /gene="nrdI"
                     /locus_tag="ONT85_003049"
     CDS             complement(10127..10573)
                     /gene="nrdI"
                     /locus_tag="ONT85_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HCR2379923.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            10784..12208
                     /locus_tag="ONT85_003050"
     CDS             10784..12208
                     /locus_tag="ONT85_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR2379924.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            complement(12389..13162)
                     /locus_tag="ONT85_003051"
     CDS             complement(12389..13162)
                     /locus_tag="ONT85_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HCR2379925.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(13159..14064)
                     /locus_tag="ONT85_003052"
     CDS             complement(13159..14064)
                     /locus_tag="ONT85_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR2379926.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            complement(14218..14538)
                     /locus_tag="ONT85_003053"
     CDS             complement(14218..14538)
                     /locus_tag="ONT85_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HCR2379927.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            15012..15698
                     /locus_tag="ONT85_003054"
     CDS             15012..15698
                     /locus_tag="ONT85_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HCR2379928.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGIASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            15700..16644
                     /locus_tag="ONT85_003055"
     CDS             15700..16644
                     /locus_tag="ONT85_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HCR2379929.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            16843..17487
                     /gene="pcp"
                     /locus_tag="ONT85_003056"
     CDS             16843..17487
                     /gene="pcp"
                     /locus_tag="ONT85_003056"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HCR2379930.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            17566..18843
                     /locus_tag="ONT85_003057"
     CDS             17566..18843
                     /locus_tag="ONT85_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2379931.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
BASE COUNT         6069 a         3328 c         4000 g         5491 t
ORIGIN      
        1 ctggagctaa gttgtttttt caatttttta tcttccataa atgacctctt tcttttaaat
       61 tcacaatcaa aatttatatt aagaataaat agcaatattt aacaatgact aatgataaca
      121 aattttcaga caattacaac tattcagaaa acttttaggc gaattttaat agaagaatga
      181 tttatttcat catattttca tacaaatgtt aaattttcat acaaatatag gaaacgtttt
      241 actggctttt tactatttaa acaggctatc ataggaaaaa gtgacagaga aagtgaggcg
      301 gaaaagatga acaaaaatga cgtactgcta gttgcaaaaa ataaatcact ttattttttg
      361 ccttccatcc gctctttaga tgtttggtcc ggaaaagatt tactacagtc ggaattcatt
      421 aaatcagcta gacaacagtt ggcaaattat cagctgatcg tttttttaga ttatggattt
      481 gatatggaga tggctcgacg cgtgcgccca tttaccaatg caaaaattgt gttatttttt
      541 tggaatcact tcaaagcaga acacgaggcg atgctgaagc aggcacaaga tgaaccggca
      601 atcgatgaga tttatcattt tgattttttg gaagcaaaag aacttgggct gaaacataat
      661 agttcatttt attcgaaaca tatgaatttt tcttctagcg aaccaaagac ggatctattt
      721 tttggtgcaa cgaataatgg gagaaaagaa cgagcagaaa gctataagca ggaatttata
      781 aaaagagatg ttacgaccaa ctatttcatt cttccacatc gagggaatga acaacctgga
      841 tatcttactt atgaagagta cctgaaaaaa acaggggaat cgcgcgggat tctggaactg
      901 ttgcgggagg gtcagcaagg agtgactttg cggacatttg aatccatgta ttttcaaaag
      961 aaactagtga cggataatca agcgattgct cattatcatt tttatcgtcc tgaaaatatt
     1021 tttttgcttc gagaacgtga tttagatgaa ttaccggaat ttctgcatac accatttaag
     1081 ccaatcctat cagagctatt ggaattttat gatgcagaaa attgggcaaa acgatttatc
     1141 actaacgatt ccactattta tgagcaatac gaatatgacc tctcctctgt tgaaaacaaa
     1201 taaaaaacct acattttgaa gagattatgt actgaaacaa acaagcacaa ttcttatggc
     1261 atcatgagag ttgtgttttc tttttgatct tttattgggc ttaaaaaaag agaaccagct
     1321 gctgattcag ctgattctca tcagagagcc ttttattctg cgccaaggat tccttctttt
     1381 gcatggacta attgggtcaa aaaggcacaa tatggtgtag caatatcata tttttgtcct
     1441 tttcgccaaa tggcaccatt gatgtagtcg atttcagtca aacgatggtt tttgatcagg
     1501 tcttggtaca tagatgggta atgcaacccg atgttgtcag gatcataagt agaagcaata
     1561 tgttggtaca cttcttcttg atctagtatg attccttctt ttgctgcgat agaagcaaat
     1621 tcagatacga ttgttcgaac catgctctct gccgcttttt gcgcgcctag ttcagcaata
     1681 ttacaatcca aaatcgtgca taaaccattc aatgttccat ttacacaagc ttttcgccaa
     1741 atggaataac gaacgttatg gctatagatc ggatttaaat ttgcttcttg aaagacttct
     1801 acaacttttt ttgtaaatgc ttcgccttct ggttccaagt tctctagttc gatttctcca
     1861 tcacctaaca atgtgacttt tccgggacca gctagaccag ctgtccacat cgtgatccca
     1921 aataagatat ttttctttgg cacatatttt tccagtacat cttcatgacc taatccattt
     1981 agtaaacaca aaacataagt attgtcagta ataatggatt ggatcgaaca aaacatatca
     2041 tctaattgat tggcttttgt taatgctacg atcaagtcca cgtgttcgtt gctttcgatg
     2101 atttcttctg gtgaatagat tggcagtttt gcgaccactt ctttaccgtt aaagtcagca
     2161 atcaacccat ttgtacgaat ggcttcaatg tgagcaggcc aacgatcgat caagaggacc
     2221 tcatttccgc tttgatgtag catcaatccg attcggctgc ccattgctcc ggccccagct
     2281 attgcgattt tcatgttttt tctctccttt attcaatgag ttccctttta gtatatagaa
     2341 aaatggaaga aaatagcatt gttttgtttg aaaaaaatga aaaaaagtgt cttacttaag
     2401 aagaaagtga gggatacaga tggatatttt ggcggcaatt gattcattta aaggaagtgc
     2461 ttcttcattt gaactaaatc aggcagttct aaatggctgg gaagatcgta aaggaagaaa
     2521 aatcaatgtg ccgattgcag atggcggaga aaatactgca gaagcaatct attacgcgct
     2581 caggggaaca tggcgtacca ttgatgggat ggatctctta ttccgtcagc gaaaaatcag
     2641 ctatttattg acaacttggc aaggaaaaaa caccgcagtc atcgaatctg cacaagcatt
     2701 ggggttagat ctacttgata aaccaacgga tcaaactatc cgacttgctt ccagttatgc
     2761 cttaggagaa cttattaaag atgcactgct tcaagaagta gaacaaatta ttgtcacttt
     2821 aggaggaagc gggtgtagcg atggtggtct aggattactc caatccttgg gtgcttcttt
     2881 agaagggtac acagaaggaa atcctttatt atctactaaa aaacttcatt ttgatcgaag
     2941 aaacgttctt gtttctaaag taaaattgat tatcgctgca gatgttacca atccatatac
     3001 aggtcaacaa ggagctgctt ggcaatttgg aagacaaaaa ggaggaatag atacgacgct
     3061 agctttctta gataaacaag caggaaagat tgctcagcaa ataaaagatt tctgggaggt
     3121 ggatttagat gatattccag gaagtggagc agcaggcggt attggtggag cccttttttt
     3181 gttgggagcg aagatggttt ctggtttcga tttagtcagc caaatcactg gattggaaaa
     3241 aaagatcgaa caagcagatg tcatcattac aggagaaggg cggatcgatc agcaaacgat
     3301 ccacggaaaa gttccatacg gtgtagcagt gcttgcaaaa aaatatcgaa aaccaataat
     3361 cgctatttgc ggaagtagag accaagagct agaaggactt tctgattttc tcccaattgt
     3421 cttaagtatt catttgggtc caataggttt agaagaagca atggaacaca ggcaaacatt
     3481 agaaaaaaca aaaatcttag gacagacact tagcagattt ttcaccatcc tcccgaagta
     3541 gttgacaaaa tcattttctt tatgctatcg taatttcaaa tataggacaa agtgagggat
     3601 ccgtgcattg aaaaattaag tcgattgatg aaagctgcat tcagactgta ccttgtggag
     3661 acaaggaagc agtgtgcaga ttttcgggat tggcttggtt ttttcgatgt atgggtcttt
     3721 ttgtgtgtat ccaacccttg catcctcttt agccagttct gaaaatagga ctggtctttt
     3781 tttacgaggt gatggaaaat gtctaaaatc gaaataaaaa atctgacatt cggctacgac
     3841 agccaaggca cattattatt tgaacaagca aatctaaatt ttgacacaca atggaaacta
     3901 ggacttatcg gacgaaacgg tcgaggaaag acaactttac tgaatattct acaaaacaaa
     3961 ctaccttatc aagggcaagt aatccatcag caagaatttg cctatttccc gcaacagaca
     4021 aaagataaag aacgtttaac ctattacgtg ttaaatgata ttacggattt tgagatatgg
     4081 gaaatcgaaa gagagctcca attgatgcaa acagatcctg aaatcttatg gagagaattc
     4141 agcacactat cggggggaga gaagacaaaa gtcctactgg cacttttatt tgtggatgac
     4201 actcatttcc cgttaatcga tgaaccaacg aatcatttgg atatctctgg tagaaaacaa
     4261 gtagcggctt atttgaaaaa gaaaaaacaa ggcttcatcg tggtcagcca tgaccgggga
     4321 tttatcgatg aagtagtgga ccatgtttta gcaatcgaaa aaagtcaact ggaactttat
     4381 caagggaatt tctctatcta tgaagaacag aaaaaacttc gtgatgaatt tgaaatggct
     4441 caaaatgaaa aattgaaaaa agaagtcagt aggctaaaga aaacagcagc tgaaaaagcc
     4501 gaatggtctc gttcccgaga aggagataaa acaaagaaac aagtcggatt catcgatact
     4561 gaatctagac gagtgaataa aggagcagtg ggtgctgatg ctgcacggac gatgaaacga
     4621 tccaaagcaa tcgtgaatcg gatggagacc cagatcagcg agaaagaaaa actattaaaa
     4681 gatatcgaat atatcgattc tttgacgatg aatagccaag cgtctcacca taagcgactt
     4741 ttaagcgtag aagatcttca attagggtat gaaaatctgt tattcgagcc aattcatttt
     4801 acaatcgagc ctcatcagcg ggtggcgatt tcaggtccta acggtgcagg aaagtcatcc
     4861 attatccatt atcttctggg ggcattcaac ggcaaggtta taggagaaaa ataccagcca
     4921 aaacatctga gcattagtta tgcaagccaa aattatgaag acaatcgagg aacgttggcg
     4981 gaatttgcag agaaaaacca agtagactac caagcatttt tgaacaacct ccgaaagctt
     5041 gggatggaaa gagatgtttt tcataacaag atcgagcaga tgagtatggg ccaacggaaa
     5101 aaagtggaat tggctaaatc tttatcacag ccagctgaac tatatacatg ggatgaacca
     5161 ttgaattatt tggatgtctt caatcaagaa caattagaac aactgatctt gaacgtgaaa
     5221 cctgccatgt tactagtgga acatgatcaa accttcctgg ataaagtatc tactgagatt
     5281 atttctcttg agagaatcta acccttgtaa tctcaagaac aacaaggtat ggttaagggg
     5341 aactttgcca acgaaggaga gaatcacatg ctttatgtaa cgagacacgg agaaacaact
     5401 tggaacgcac aaggattagt ttgcggacgg gcagatgtgc cgctgactga aaaaggacag
     5461 atgcaggcac agaaactagc agaaaaggta gtagatttgc ctgttccaat cacgaaaatc
     5521 atccattccc ctcttcaacg agcaagagat acagcacaag cagttgctga tcgtctcagc
     5581 ttgccgctga ccgttgatga acgattggta gaaatggatt ttggggatta tgatggactg
     5641 ccaagcaaag atgagaactt tcaaaaagca cgtttagcgt tcgctgtacg attcccaaat
     5701 ggggagtcag tcttagatgt gtatgcacgg atcgttcctt tattgaaaga atgcatcgaa
     5761 gacgaagaaa atgtctatct gttagtttgc cataatgcct tgataagagt gatcaatgct
     5821 tattttcatc cgatgcctaa tgaggggttc tttacattca tggtagataa tacagaattg
     5881 gtttcttatg agtaattatg tttttgtgtt atttaaataa aatatttcaa ggcactgctt
     5941 ttgcttagca gtgccttttt taggttatga caattctttt gataatataa actcaacctt
     6001 tagaaatgaa atttaattat tttaataaaa gtattgaaat tttgtttctt tagatatata
     6061 ctctagtcat agaaatgaag acggatacag aaaaagaggg ataatatgtt tggcttttca
     6121 atatttatga acgattcttt gtcagaagag aagaaacagt atatccgcca aatggcatcc
     6181 aatggctttg tcggtatttt cacttccatg catataccag aagatgatgt ttcggcatat
     6241 caaaagcgat tgatcgaact aggaagcgaa gcgaaaaaga ataaactaga attgatggtc
     6301 gatatttcag gagaggcatt ggaacgagcg ggtttttcat tagaagatct aacgcctttg
     6361 aaagcaattg gtgtaacagg tttaagaatg gattatcata tttccaatca gcagattgcc
     6421 gactggtcac atcaattgaa aatcagtttg aatgctagca cgatcacgcc taaagatatc
     6481 gatgaattga aagaagcaga agctgatttc tcacagattg aagcttggca taattattat
     6541 ccacgtcccg aaacagggct ggacaaagaa tggtatcaga aaaaaaatca atggctcaaa
     6601 attcaaggtc tccttgtcca aggttttgta ccaggagata cagagttgag aggcccacta
     6661 tatcaaggat tgccaacttt ggaagaacat cgagggatcc atcctcttgc aggcgctcta
     6721 gatctattag caagtaacac ggatattgtt tacatcggag atgcaggtct gtcagaaaac
     6781 gttcaggagc aatttgcttc tttccaaaaa gaacagacag tcttgctcca tacggaacct
     6841 gtagatgaag aattttacga atatatatta gggaagcata cgaatcggca agacgatgca
     6901 agagatgtca tacgaagcgt agatgcacgt tttcgagaga ttccgcctat cccagcaaga
     6961 aatacagcta cgcgtatgaa agggtctatt actttagata acgaaaagta tttgcgctat
     7021 atgggtgaga tccaattaac caaatatgat ttgccagcgg atgaaaaagt gaatgtagta
     7081 gctaaagtta tcaaagaaga gctaccattg atcaaccaaa tcaaagctgg tatgaactat
     7141 caatttataa gaaaagaagg aagataaaaa tgagcaaaat caatttagag aacctaacaa
     7201 cggaacgcag aaaccaagaa acttttggat tagatgagat gagtatttct caagccgttc
     7261 aactaatgaa caaagaagat cagaaagtgg ccgctgcggt tgaaaaagaa ctgacaagta
     7321 tcgaaccagt gattgctaac acgattgagt cctttaaaaa aggtggacgt ctgatttata
     7381 tgggagccgg aacaagtgga agactaggtg tgttagatgc tgctgaatgc gtaccgacat
     7441 tcggtgtaga accggaaatg gtcataggtc taattgctgg tggagaagaa gcaatgactg
     7501 tagctgtcga aggggccgaa gattcttatg agctagggca agaagatcta atcaagctga
     7561 atttgacaaa aaatgatata gttattggta tcgcagctag cggtcgtaca ccatatgtga
     7621 tcggtggatt agattacgca aaatcgatcg gtgctgtcac aggaacgatt tcttgcaata
     7681 aacaagccga aatcagtaaa cacgcggatt tcccaatcga agtcgatgca ggtccagaat
     7741 ttttgacagg ctctactcga ttgaagtcag gaacagcaca aaaacttatc ttgaatatga
     7801 tttctactat cagtatgatt ggtataggta aagtattcaa taacttgatg gtcgatgtca
     7861 aaccaacaaa tgaaaaacta atcgaacgct caaaacgaat catcatgcaa gcgacagaag
     7921 ctgattacga aacagctgaa aagtactttg aagaagcaga tcaaaatgtt aagctggcta
     7981 tcgtcatgat tttgacaaat agtgataaag aggaagctca agcaaaatta gctgaagcaa
     8041 acggatttgt aaaagggacc ttatgagggg gaaaagacca tggcaaaaaa cgaactgact
     8101 ttggaagaac tggcaaaaca aatttataaa ggggcaggcg gcatgggcaa tgtagaaagc
     8161 attgtccact gcatgactcg tgtgcgtatg aagatcatca atgatgatct tgtaaatgtc
     8221 tctgaattaa aagcgattcc aggagtgctt ggtgtagtag aagatgaaca gcttcaagtc
     8281 atcatcggac ctgggaaagt gaacaaagta gcacagcaaa tggttgattt ggcaggcgtg
     8341 aaattaggag aaaaactttc ttctgtaagt acagctacag caaattcatc agcatctgga
     8401 aaagaccgtg taaacgaacg ggcacaagaa atgaaagcgg cacaaaaatc gaaacaaaaa
     8461 ccatctaaaa tcaaatcggt attaaaagac atttctaata tctttgtgcc aatgattcct
     8521 gcatttgtcg gtactgggat cgtggcaggg attgccgcag ttttagcaaa cttagtcaca
     8581 gccggaacac ttgatgcagc aacatggcaa caatatatcg atgtgatgaa catcttgaaa
     8641 aacgggatgt ttagctacct agttatctat acaggtatca atgctgcaca agtttttggt
     8701 gcaacaccaa ctcttggtgg agttatcggg gccgttgtga tgctgacagg gatgaatccg
     8761 gaagcaccac tgaaaaactt atttactggt gaagctcttg cagctggaca aggtgggatc
     8821 ctaggtgtca tcttcgctgt ttggctattg tcaatcgttg agaaaaaact gcataaagta
     8881 gtacctgatg cggtagatat catcgttaca ccaactcttt cattgattgc catcggtctg
     8941 attgaaattt tcttcatcat gcctttagca ggtttcattt ctgatggcat ggtcggtggg
     9001 atcaactggg tacttggcgt tggtggagca tttgccggct tcgttctagg cacattattc
     9061 ttgccgatgg ttatgttcgg actgcatcaa attttgactc caatccatgt gcaaatgatt
     9121 gatgaaactg gtcgtacgct attgcttcca attcttgcga tggctggtgc aggacaagtg
     9181 ggtgcagcat tggcattatg gattcgctgc aaaaaagata aagaattaac agaaatgatc
     9241 aaaggagctt taccagtcgg gatcctagga atcggcgaac cattgattta cggagtaaca
     9301 ttacctctag gacgcccatt tattactgct tgtatcggtg gaggtatcgg cggggcagtg
     9361 atcggaatga tcggaaatgt cggggctatc gctattggac cttcaggagc cgctttgatc
     9421 ccattgatct ctgacggtaa atggttaggc tacatccttg gattattggc agcttatgct
     9481 ggtggcttcg ttgctacatt cttcttcggt attcctaaag accgtttaga aaaagaagaa
     9541 ttagcgactg aaacagccac agtaaatgaa gtgcctgaaa aagtagtacc tcaaacttca
     9601 gctgaaagtg tgactatcct ctcatcagtt gctgacggaa cagttgtttc tttagataaa
     9661 gcaagtgatc cagtttttgc acaaaagatg atgggtgaag gctactttgt agaaccagaa
     9721 aacggacata tctattctcc agttactggg gtagtcagct ctgtcttccc aacgaaacac
     9781 gctattggga tcacaacgcc aaatggttta gaaatcttgc ttcatatggg aatcaacaca
     9841 gttgacttag gcggtacgcc atttgatgtg aaagtaacag aagggcagca agtgacacct
     9901 gatacgcttg tagcagaagc tgacttagaa gcgatcaaag cagcaggcaa agaaacatcc
     9961 atgatcgtct tggtaaccaa tatggatcgt gtaaaaaact ttgtcttgga aaaaacaggt
    10021 aaagcaaaag caaaagcacc tgtgatggat gtcaaagctt aatatgatag aaacaataga
    10081 aacgaaagaa gaaactgaaa tctatttttc agcttcttct tttttattat ttattgtttt
    10141 ctgctaaaat ctgataaatt cgtgtcacat cagatggtgt tccccgtaat tcataatctg
    10201 ccaagaaagg aaagccaaat gtttgtgcat attgtttggc tgttagacaa tactgattat
    10261 tgaagttttt atttccgcta ccgactacac caagacaaag tttatgattg tcttcatgtt
    10321 ccagatattc acgcatcgtt tcggtcaaga tttctgtatc accgttatct aaaccatttc
    10381 cgccgtctaa ataagtagga acgaaagtaa aaaatggttc tgtttctttc gtaaaatcac
    10441 tattttcatg gatttctttt aaagtaactt caggattgac aggatcttct gcatgcattt
    10501 tttcagcata ttctgctaga tgtttggcaa aggcacgcgt atttcctgaa atcgatatat
    10561 ataaaatgtt catggatcat cctccaattc tttcctttta gtatagcatc ttttttaagt
    10621 tttttggtgg tgtaatttgg aaagggacta ggcgctttgc ttgtataatg agaaaaaagc
    10681 cggtagaatt atatttctat taaagttttt attaattaag taaaaataaa aaaggtttgt
    10741 tatacttttt gataaaagcg gttttatttt ggaggatgct tacatggata cagagcaaca
    10801 gaaaaaagga attggtttaa ttccacttgc tgccttagta attggttcag cgattggagg
    10861 tggcgtattt ggtattatga cggacatctc cggatcggct ggcggcccag ctattttcag
    10921 ttgggtactc gtaggaacag caatgttgat gctctccttg tcgatcaata atatcaataa
    10981 aaaacgtcca gatttaaaag gcgggatctt tagttacgca gaagaaggtt ttggccgttt
    11041 cgctggtttt atcagcggtt ggggttactg gctcacatgc tggttaggca acgtagcttt
    11101 tgcaacattg ttgatgagcg cattaggata tttcttccca gtatttgaag gtgggcaaaa
    11161 tctggcatcg atcgtgatga gtacagtctt tttatgggga tatgcatggc tagtgaatcg
    11221 gggagtcgaa aatgctagtt tcgtcaatgc tatcgtcaca ttctgtaaat tagtgccatt
    11281 atttttattt attgtagtag ctatcagcac gttttcgatc caacaattca ttgataactt
    11341 ttctagcgac attatattga atgcgaatgg acagccgatc ccgttttcca gtcaagtatt
    11401 gagttgcatc atggtgatgc tttgggtatt tgttggtatt gaaggagcct ctgtcgtagg
    11461 ttcacgagca aaaaaacaat cagatgttgg gaaagcaacg attctaggga tattcggctt
    11521 gattattatt tacgtcttgg tttccatgct tccatatggg acaatgacga tgaatcagct
    11581 gcaaacgatc aaggcgcagc ctgctatggg ctatgtgttt gaaatgatgg ttggtaaatg
    11641 gggagcagtc gtgatcaacg gcggtttgat catttcttta gttggtgttt ggctctcatg
    11701 gacgatctta ccgattgaaa caatgggaaa catggctgaa gacggacttt tgcctaaagc
    11761 atggggaaaa ctcaacaaaa atggggcacc aacttatgct atcgtattga cgacgttatg
    11821 tacgaatgtc tttttacttt cattattatt cactgacagt gcttataact ttgcttacac
    11881 acttggtaca gcagctattt tcttcacgtg gcttttctta ggattgtacc aaatgaaact
    11941 ttcttatcag cggaaagaat gggtccaatt tttagtaggg cttttagcaa gtgcctttca
    12001 agtatgggct atgctagtcg tagcctttaa tgaagtaatg cttgttcttg ttcttttctt
    12061 gccaggcatt tatttttacc tcaaagcacg taaggagcaa aacgagcctg tacaacatat
    12121 ccacttagat aagattttac ttataggtgt gacgatggtc ggagtgattg caatggtttt
    12181 atttgccact gggatgcttc acgtatagaa gtataaaaga agtataaaga gaggttgtga
    12241 gcctgtcgtt tattcaaaaa catactagga aaggagctgt gacacaaatg ttgtcacagc
    12301 tcctttttca gaataaacgg tgtttaagag acagccaatc ggcaataagg ctgaacgtct
    12361 ctttcacagc ctcttttttg atgagtcatc atacattttt tcgcttaaat acgagatatc
    12421 ctaatgctaa gaacaaaacg atgtaagcta aattaccgaa tagcatttga ttagtagaaa
    12481 gatgagtcaa ttgcttcatg atctcttcgt ttccaatttg gttttgcaga ttcaacatac
    12541 tgatcggatt ccatttgatc cactcccatt tttggatgag tgcaataagg ataccagata
    12601 tgattgacga agcaaaataa aagacgattc ctgctgagat ggatgcgccg gaactattga
    12661 taaagcaagc aatcatcaac actaaactta aaatcagcca taaaccgata taactaccca
    12721 aagtataagt gaacaagctt tggatcagtg tttgaccagt agaaaccttc tccgtgaagg
    12781 aaatcgaagg gaaaagaatc agtttcatca acacagtgac aataatagct aaaaggtata
    12841 aataaacaga ataaataact aatgtgatcc atttgcttac taatatttct cctcgagaat
    12901 actttcgata aaggagattt ttcaatgtac catattgtgc ttccatcgaa atgatcgtgc
    12961 ttgcagctgc aatcatgata aagacaatcc aagatgtggc actaaaaagc tgagcagtca
    13021 tttcaactgg atcaatgatc gtcgtgtatt ttttggctag aagtgctgtt cctatgagca
    13081 aaacaactaa tagaacagat aaaacagcag ttgacttttt cttgatcaat ttaaataatt
    13141 cttgttttac aagcaatgtc atttttcttc ttcccccttt actgaatcca gaatttccaa
    13201 tagagaagat tccaaatcat tcgtcagatg ctggacgtct gtcacatgga tattttgtgc
    13261 agacaatagc tggatcacgg ttcctaataa ttcttcgttt tcttttaaaa gcttgatttt
    13321 atcaggatct tccaaagtat atccagcttc tgtcaagatc gttcgagcag ctagatcatt
    13381 tgttgtagag ataacgatga aatcttgcgc tgtttggttc aattcagcca ttgtacattg
    13441 tttgatgatc tttccttgat tgatcaggat cagctcatcg accatttttt caagctcgct
    13501 gagtaagtga ctggagatca agaaagaggt gccctgagta gccaagtcgg taatgatttt
    13561 tcgcacatct cttgttgctt gtggatccaa accgttcatc ggctcatcta aaataacgat
    13621 agacggatgg ttcagtaagg caagagcaat tcccaatttt tgtttcattc ccaacgagta
    13681 ggatttcgct tttttgtgaa tgtacttttc catcttgagc tgattcacga tagtgtcgat
    13741 agcagataca tcgttggttt cagagaataa cttcagatga tcatagcctg ttaagaatgg
    13801 atagataccc gggtattcga tcaatgctcc tacttgttcc aaccctttat gactagaggg
    13861 agaaatctca ttttcaccaa tacagattgt tccatctgag taatggatca gtcctaaaat
    13921 agctttcatg atcgttgttt ttccagcgcc attaggacct actaatccaa cgattttgcc
    13981 aggggataag ctaaaactga catcgctgag ggcttgtttg tgaccaaagt tcttggataa
    14041 ttcttggacc gaaagacttt tcaaaaatat tcactccttt tctatagata gtacaagcat
    14101 acacctgatg agtggagaag tcgctaggtc attggactta tttcttattt tttcataaaa
    14161 aaattgaata ttaaataaaa aggcttattt tttctgatca gtacttattc caagtgtcta
    14221 gtggccgcta gtaatcttga tgccaatgat actgatgagt agcatcccca caaagaacca
    14281 agtggaaaga ttcagcttat catgaaacaa gaccacacca attatgaccg aaccaactgc
    14341 gccaatccct gtccagatag gataagagag actcatcggc aaacttttga ttgcctttga
    14401 caaaaagtaa aaactagcaa tcatgccaat cactgtgtaa aaagaataat tgattttaga
    14461 aaaaccatca ctccatttca tcatcgttga ccagaatact tccaaaattc ctgctatgat
    14521 taattccatc catgccatac ttacattcct ccttaactat aaaaaaatac acaaaaagag
    14581 gaaaatcatc ccttcaaaga cctaatggaa tgatcatcct ctttatccta cccggtggca
    14641 gattgtatat ctttttctgt agtatagcac acctatccag atatcggcaa tctcatactt
    14701 tttagtaaag tgtaccaagc actgacggat atatcgttag cggaaaagaa gatgaatgct
    14761 ttgcaatttc atagaaagaa gttgttattt attaatcatt taaaaaaaac agtaactaga
    14821 ccttatttct atatattatc gggcaataaa ttaaaaaaag ctttacctta caataagtaa
    14881 gataattttt gtgataaaaa aataacgaaa aatatttaaa aatagtgtct atttcgtgag
    14941 aaaacaacta tattttttat gtgttttgct ttataattaa aaaaatattg tcattttatg
    15001 gaggaaaata tatggaaaat tatcttagcc ttttaggcgt cttgattgtt attctcgggt
    15061 ttgcatttaa attagactcg atattgatcg taatggttgc tttagttgtt acagctttga
    15121 ctggcggtct tggcattcaa ggaatgttag aacttttagg tacaagtttt gtaaacaatc
    15181 gcgggatggc gatttttatc atcattatgt tggcaacagg aacgcttgaa agaaatggtc
    15241 tgaaagaatc agcggcttcc ttgatcaagc gattcaagaa agtatctgct ggtatgattg
    15301 tagatattta tgggatcttt cgaatgatct ttgcggcttt taacgtgagt tttggtggag
    15361 ttgctggatt tgttcgtcca atcattttac cgatggcatt ggggaccatc gaatcaaaag
    15421 ggttagaagt ccatcctaag catgaagaag aactaaaagg tatagcttca gctatggaaa
    15481 atgtctgctg gttctttggc caagtactat tcatcggagg agcaggggcg ttgctagtcc
    15541 aatcaacact gaaagatcta ggatatgacg taacattggg acagcttgct ttagtggaag
    15601 ttccagtggc tatcacggct ttagttgtgg caagtgtcta cttctatctt aaagaaaaga
    15661 aattagcgaa gaaatattat ccaggaaagg ggcaataaga tggacttttt tacaaatcct
    15721 gatattttat taggcgataa attactggag atcctttata tcgtgatggg actcgtattg
    15781 atttacactg gagtaagaaa tttaacagat aaaaccaatc ctcatcgtta tgggtcagcc
    15841 tttttctgga cggtcttagg ggtagttatt gcaggaggaa gatggcttcc ggcaatcgtg
    15901 aacggtggct tgatctttgc gatgaccata ccagcaattg ctaagaaagt cagcaaagga
    15961 gaaagccggc ttccatcaaa agaatatatg gaaaaaatgt cagacaaact aggaatgaaa
    16021 atttttattc cagctttgag tatcggtgtc tttgcgatct tgtttgctct atttaccaat
    16081 ttaggtgctt tagtcggcgt gggcgtcggt gtttttgcag ccatgctaat cttgatgttc
    16141 ttttctcgag ataatcgacc aagtacattt ttagatgatg cagcagatat gctgggcaca
    16201 gtagggccgt taagcatgtt gccaatgctt ttagcttctc taggtgcggt atttacaagc
    16261 gctggagttg ggacagtgat ttccaatgca gtaggtacga tcgttccaga aggaaatgtg
    16321 aacatcggca tcatcattta cgctcttggc atggtgatat ttacggtaat catggggaat
    16381 gcatttgcgg ccatcaccgt gatgacggtt gggatcggtg caccattcgt atttagtcat
    16441 ggagcgaatc cagcgttaat aggaatggtt gcattgactt gcggtttttg tgggaccctg
    16501 ctgacaccga tggctgctaa cttcaatatc gtgcctgtag cgatgctgga gatgaaagac
    16561 aaatacggag tgatcaaaaa ccagttgttt attgcgttat ttatgctagt tttccaaatt
    16621 gcttatatga ttctatttaa ataatagaag agaggggttg tgacagaata attgtcgcaa
    16681 cctcttctaa cattgtagtg gaacactatt tttttataag aatatattaa gtttgatctg
    16741 ttatgttatt taaactattg tttcctttat ttacgctgtt caaggaaaat ggtatgataa
    16801 aagataacca tatgttaaat caatcggacg gaggaacaac agatgaaagt actagtaaca
    16861 ggttttgatc catttggtgg ggacaaagtg aatccagcct atgaagcagt aaaaaaaatg
    16921 ccagatgaaa tcagcggtgc tgaaatcatc aaagtggaag taccgaccgt attcgaaaaa
    16981 agcagtcaag tagtcaaaga agccatccaa cagcaccaac cagatgtcgt gatctgtgtc
    17041 ggacaagcag gtgggcggtc agcagtctcg ttcgaacgtg tcgcaatcaa tctagcagaa
    17101 gcaagaatcc ctgataatga aggaaatcag ccattcgata cggctttaga agaaaacggt
    17161 ccagccgctt actttacctc attgccaatc aaagcaatga caaaaaatgt acaagatcat
    17221 ggtctaccag cgtacatctc ttatacagca ggaacatttg tctgcaatga tatcatgtat
    17281 cgtttgcttc atttgatcga aacacaattc ccggcaattc gaggtgggtt catccacgtg
    17341 ccattctcac ccgatcaagt gatcgatcgt ccagtgggta cgccttctat gtctttagaa
    17401 gacattgctt cttctttgac ttatgcagtt gaagcagcag tagaaaataa agaagatatt
    17461 tccggaaatg ccggaacaac tcactaattt agcaagaaac actggctggt tgctaacgtt
    17521 atgttagtag ccagcttttt gtaattagaa aggaaagaaa cagccgtgga agaatggcaa
    17581 tccgtctttg aagaatggtt tccaaaagaa atcagcaaaa gctatcccat caaaatttct
    17641 aaacaataca caagcagtca acgttgggag atctatgcaa aattaacgaa aaaacagcgg
    17701 gaactagtcg ataagcaccg ccgatatcta atcagctctc gttttatgga agaacattat
    17761 ttagctgcaa cagattgggt gttttctgac ttcaagatca atccgttttt tcgaacaaaa
    17821 cgaagccagc aaaaattgta ttgtgagtgt ggtagagaat taaaagtgca atacatcgtt
    17881 aaatcgccaa aaaccggaaa gatcctaaaa ctaggcatca accactttgc cgatcacctg
    17941 catgtctctc cgacagtggc agcttcgatc catcagggaa tgacaaaagt tgatctggcc
    18001 ttagacgaat tattatggct taagcagaag aatatcgatt ttcctgaagg actatggcaa
    18061 aaatactgtt ttgtcttgta ccaaaatcgt cggatgaaac agccatattt accggatata
    18121 aaattagctc agcggctcgc agaatttcgc caagtagaaa tgccgattta catagcggat
    18181 tatcaagcat tggaaaatga aatcaagaaa ataagtgaac acatcaatgg gcagtcaaaa
    18241 aagcgacaga taaaaaaaga actatttgac gactttgcag aagaattagt aaaggatgta
    18301 gaagaattct tgatcaacta tcgcgcattt ttacgtaaag attggcaatc catcgtatat
    18361 gaagaagtgc cagtacatcc taatgcttat tttgaaacat ttatttccgt gttgagaaag
    18421 acaaaacgtc aacgaacacc agaagtcact gctcagatgg aatattttgc gaaaaaccaa
    18481 cgattcatcc aaccaaaaat ctatctattt atttggaagc aatattgtcg ctatggtttt
    18541 acagaagggt tcttcgacag cattcctcgg atagtcagaa atggtttttt aaaagtattg
    18601 cgtaaagaac gagaagctat acaatctgct gataagaaag accgtacagt ctcaaaagaa
    18661 aaatggcaac tagtagtgaa agatatccaa tctggaaatg tgcaagagac aatcgacaaa
    18721 tggaaaggaa aacattatcg ttttactgaa gctcagaaac aagcgttgga atattatcag
    18781 aagttagaag aatcactgcg tttcaatgat gaggcaagaa agtatctgaa ggaattacta
    18841 taaaaaatag aggctgggac agaagcgttt aactccaaga aataagaa
//
LOCUS       DAJSBL010000215         5873 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.guided.100, whole genome shotgun sequence.
ACCESSION   DAJSBL010000215 DAJSBL010000000
VERSION     DAJSBL010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5873
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.guided.100"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(41..1027)
                     /locus_tag="ONT85_003058"
                     /pseudo
     CDS             complement(41..1027)
                     /locus_tag="ONT85_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS1542 family transposase"
     gene            1179..1874
                     /gene="vanR"
                     /locus_tag="ONT85_003059"
     CDS             1179..1874
                     /gene="vanR"
                     /locus_tag="ONT85_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HCR2379932.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            1852..3006
                     /locus_tag="ONT85_003060"
     CDS             1852..3006
                     /locus_tag="ONT85_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HCR2379933.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
     gene            3221..4189
                     /locus_tag="ONT85_003061"
     CDS             3221..4189
                     /locus_tag="ONT85_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HCR2379934.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            4182..5213
                     /gene="vanA"
                     /locus_tag="ONT85_003062"
     CDS             4182..5213
                     /gene="vanA"
                     /locus_tag="ONT85_003062"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HCR2379935.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            5219..5827
                     /locus_tag="ONT85_003063"
     CDS             5219..5827
                     /locus_tag="ONT85_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HCR2379936.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPKRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT         1772 a         1144 c         1320 g         1637 t
ORIGIN      
        1 gggattttat tctttattta aaactttgca acagaaccct atttccgata accgtgtatt
       61 gaaaggcatc ttcgaagcca ttatccaata tttcgcaggc ttttgtatat ttaggctggt
      121 cgatatattc accgactaag gcattcttag ctgttcgagc gagttcaata tccgtaaact
      181 taaagatcgc ttttactgct tctctaaacg gttttgaatt cttttttgga atggaactga
      241 agatgtttct taaaaaatgg acctggcatc tctgccaact tgcgttggta aatgacttac
      301 gaatcgcaga cactaggcct ttatgggcat cagaaatgat gagttctgtc ccctttaggc
      361 cgcgttcttt taagtattca aagaagatgg accatgtgtc atcactttct tcattttgaa
      421 tcatgaagcc aatgatttca cggtcgccac cttctgttat cccgatcgca atatggcagc
      481 ttttagaaag cactcgatgg tcctcacgga cttttatgta gagaacatca gtcatcagat
      541 aaggataatt cgtacctgag agtgaacggt tctgccattc gttgaccatc gggtctaact
      601 gctcagtcag gctagaaaca aaagatttcg atacagattt tccacatagc tcttcaacaa
      661 tctttgaaac tttacgtgtc gaaacgcccg aaacatacat ctcaagcatt gaagcgagca
      721 gtgccttttc atttcgctga taacgctcaa acaccgtcgg tgaaaattca ccatcacgtg
      781 ttctaggcac ttttaattcg agtgtaccca cacgagtcgt aaagtctcgc tcataatagc
      841 catttctttg actctgacga ctttctgaac gttcatagtc atcggcttga atatattctg
      901 ttcgttgatt ttccatcaat tggttgaaaa cagtggttaa aatattttta gaaacatcat
      961 cttttaccga atgttcaata atactttgaa cctcttcgtt gttcagtgta aaatgtactt
     1021 gggtcatgta acgtcctcct gggtatgttt ttgtggttaa aaacattgta ccgtaaaagg
     1081 gctgttacat ggccttttat cttttacaca attatatgga ctttatccta taatctgata
     1141 aattattaag ggcaaactta tgtgaaaggg tgataactat gagcgataaa atacttattg
     1201 tggatgatga acatgaaatt gccgatttgg ttgaattata cttaaaaaac gagaattata
     1261 cggttttcaa atactatacc gccaaagaag cattggaatg tatagacaag tctgagattg
     1321 accttgccat attggacatc atgcttcccg gcacaagcgg ccttactatc tgtcaaaaaa
     1381 taagggacaa gcacacctat ccgattatca tgctgaccgg gaaagataca gaggtagata
     1441 aaattacagg gttaacaatc ggcgcggatg attatataac gaagcccttt cgcccactgg
     1501 agttaattgc tcgggtaaag gcccagttgc gccgatacaa aaaattcagt ggagtaaagg
     1561 agcagaacga aaatgttatc gtccactccg gccttgtcat taatgttaac acccatgagt
     1621 gttatctgaa cgagaagcag ttatccctta ctcccaccga gttttcaata ctgcgaatcc
     1681 tctgtgaaaa caaggggaat gtggttagct ccgagctgct atttcatgag atatggggcg
     1741 acgaatattt cagcaagagc aacaacacca tcaccgtgca tatccggcat ttgcgcgaaa
     1801 aaatgaacga caccattgat aatccgaaat atataaaaac ggtatggggg gttggttata
     1861 aaattgaaaa ataaaaaaaa cgactattcc aaactagaac gaaaacttta catgtatatc
     1921 gttgcaattg ttgtggtagc aattgtattc gtgttgtata ttcgttcaat gatccgaggg
     1981 aaacttgggg attggatctt aagtattttg gaaaacaaat atgacttaaa tcacctggac
     2041 gcgatgaaat tatatcaata ttccatacgg aacaatatag atatctttat ttatgtggcg
     2101 attgtcatta gtattcttat tctatgtcgc gtcatgcttt caaaattcgc aaaatacttt
     2161 gacgagataa ataccggcat tgatgtactt attcagaacg aagataaaca aattgagctt
     2221 tctgcggaaa tggatgttat ggaacaaaag ctcaacacat taaaacggac tctggaaaag
     2281 cgagagcagg atgcaaagct ggccgaacaa agaaaaaatg acgttgttat gtacttggcg
     2341 cacgatatta aaacgcccct tacatccatt atcggttatt tgagcctgct tgacgaggct
     2401 ccagacatgc cggtagatca aaaggcaaag tatgtgcata tcacgttgga caaagcgtat
     2461 cgactcgaac agctaatcga cgagtttttt gagattacac ggtataacct acaaacgata
     2521 acgctaacaa aaacgcacat agacctatac tatatgctgg tgcagatgac cgatgaattt
     2581 tatcctcagc tttccgcaca tggaaaacag gcggttattc acgcccccga ggatctgacc
     2641 gtgtccggcg accctgataa actcgcgaga gtctttaaca acattttgaa aaacgccgct
     2701 gcatacagtg aggataacag catcattgac attaccgcgg gcctctccgg ggatgtggtg
     2761 tcaatcgaat tcaagaacac tggaagcatc ccaaaagata agctagctgc catatttgaa
     2821 aagttctata ggctggacaa tgctcgttct tccgatacgg gtggcgcggg acttggattg
     2881 gcgattgcaa aagaaattat tgttcagcat ggagggcaga tttacgcgga aagcaatgat
     2941 aactatacga cgtttagggt agagcttcca gcgatgccag acttggttga taaaaggagg
     3001 tcctaagaga tgtatataat tttttaggaa aatctcaagg ttatctttac tttttcttag
     3061 gaaattaaca atttaatatt aagaaacggc tcgttcttac acggtagact taataccgta
     3121 agaacgagcc gttttcgttc ttcagagaaa gatttgacaa gattaccatt ggcatccccg
     3181 ttttatttgg tgcctttcac agaaagggtt ggtcttaatt atgaataaca tcggcattac
     3241 tgtttatgga tgtgagcagg atgaggcaga tgcattccat gctctttcgc ctcgctttgg
     3301 cgttatggca acgataatta acgccaacgt gtcggaatcc aacgccaaat ccgcgccttt
     3361 caatcaatgt atcagtgtgg gacataaatc agagatttcc gcctctattc ttcttgcgct
     3421 gaagagagcc ggtgtgaaat atatttctac ccgaagcatc ggctgcaatc atatagatac
     3481 aactgctgct aagagaatgg gcatcactgt cgacaatgtg gcgtactcgc cggatagcgt
     3541 tgccgattat actatgatgc taattcttat ggcagtacgc aacgtaaaat cgattgtgcg
     3601 ctctgtggaa aaacatgatt tcaggttgga cagcgaccgt ggcaaggtac tcagcgacat
     3661 gacagttggt gtggtgggaa cgggccagat aggcaaagcg gttattgagc ggctgcgagg
     3721 atttggatgt aaagtgttgg cttatagtcg cagccgaagt atagaggtaa actatgtacc
     3781 gtttgatgag ttgctgcaaa atagcgatat cgttacgctt catgtgccgc tcaatacgga
     3841 tacgcactat attatcagcc acgaacaaat acagagaatg aagcaaggag catttcttat
     3901 caatactggg cgcggtccac ttgtagatac ctatgagttg gttaaagcat tagaaaacgg
     3961 gaaactgggc ggtgccgcat tggatgtatt ggaaggagag gaagagtttt tctactctga
     4021 ttgcacccaa aaaccaattg ataatcaatt tttacttaaa cttcaaagaa tgcctaacgt
     4081 gataatcaca ccgcatacgg cctattatac cgagcaagcg ttgcgtgata ccgttgaaaa
     4141 aaccattaaa aactgtttgg attttgaaag gagacaggag catgaataga ataaaagttg
     4201 caatactgtt tgggggttgc tcagaggagc atgacgtatc ggtaaaatct gcaatagaga
     4261 tagccgctaa cattaataaa gaaaaatacg agccgttata cattggaatt acgaaatctg
     4321 gtgtatggaa aatgtgcgaa aaaccttgcg cggaatggga aaacgacaat tgctattcag
     4381 ctgtactctc gccggataaa aaaatgcacg gattacttgt taaaaagaac catgaatatg
     4441 aaatcaacca tgttgatgta gcattttcag ctttgcatgg caagtcaggt gaagatggat
     4501 ccatacaagg tctgtttgaa ttgtccggta tcccttttgt aggctgcgat attcaaagct
     4561 cagcaatttg tatggacaaa tcgttgacat acatcgttgc gaaaaatgct gggatagcta
     4621 ctcccgcctt ttgggttatt aataaagatg ataggccggt ggcagctacg tttacctatc
     4681 ctgtttttgt taagccggcg cgttcaggct catccttcgg tgtgaaaaaa gtcaatagcg
     4741 cggacgaatt ggactacgca attgaatcgg caagacaata tgacagcaaa atcttaattg
     4801 agcaggctgt ttcgggctgt gaggtcggtt gtgcggtatt gggaaacagt gccgcgttag
     4861 ttgttggcga ggtggaccaa atcaggctgc agtacggaat ctttcgtatt catcaggaag
     4921 tcgagccgga aaaaggctct gaaaacgcag ttataaccgt tcccgcagac ctttcagcag
     4981 aggagcgagg acggatacag gaaacggcaa aaaaaatata taaagcgctc ggctgtagag
     5041 gtctagcccg tgtggatatg tttttacaag ataacggccg cattgtactg aacgaagtca
     5101 atactctgcc cggtttcacg tcatacagtc gttatccccg tatgatggcc gctgcaggta
     5161 ttgcacttcc cgaactgatt gaccgcttga tcgtattagc gttaaagggg tgataagcat
     5221 ggaaatagga tttacttttt tagatgaaat agtacacggt gttcgttggg acgctaaata
     5281 tgccacttgg gataatttca ccggaaaacc ggttgacggt tatgaagtaa atcgcattgt
     5341 agggacatac gagttggctg aatcgctttt gaaggcaaaa gaactggctg ctacccaagg
     5401 gtacggattg cttctatggg acggttaccg tcctaagcgt gctgtaaact gttttatgca
     5461 atgggctgca cagccggaaa ataacctgac aaaggaaagt tattatccca atattgaccg
     5521 aactgagatg atttcaaaag gatacgtggc ttcaaaatca agccatagcc gcggcagtgc
     5581 cattgatctt acgctttatc gattagacac gggtgagctt gtaccaatgg ggagccgatt
     5641 tgattttatg gatgaacgct ctcatcatgc ggcaaatgga atatcatgca atgaagcgca
     5701 aaatcgcaga cgtttgcgct ccatcatgga aaacagtggg tttgaagcat atagcctcga
     5761 atggtggcac tatgtattaa gagacgaacc ataccccaat agctattttg atttccccgt
     5821 taaataaact tttaacggtt ctgttgcaaa gttttaaatc tactatcaaa taa
//
LOCUS       DAJSBL010000216         4221 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.guided.126, whole genome shotgun sequence.
ACCESSION   DAJSBL010000216 DAJSBL010000000
VERSION     DAJSBL010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4221
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.guided.126"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            100..564
                     /locus_tag="ONT85_003064"
     CDS             100..564
                     /locus_tag="ONT85_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCR2379937.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            548..1177
                     /locus_tag="ONT85_003065"
     CDS             548..1177
                     /locus_tag="ONT85_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HCR2379938.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            1531..3009
                     /gene="msr(C)"
                     /locus_tag="ONT85_003066"
     CDS             1531..3009
                     /gene="msr(C)"
                     /locus_tag="ONT85_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HCR2379939.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            3056..3502
                     /locus_tag="ONT85_003067"
     CDS             3056..3502
                     /locus_tag="ONT85_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HCR2379940.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(3701..4216)
                     /locus_tag="ONT85_003068"
     CDS             complement(3701..4216)
                     /locus_tag="ONT85_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HCR2379941.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
BASE COUNT         1385 a          706 c          876 g         1254 t
ORIGIN      
        1 tttttttatt acacagctga ttctttgact tttttctttt tttattctat tattgacaag
       61 tcaatcgttg acgagtcaat tattagaaag gaggaaatta tgtctttaga agagattagt
      121 caccttttat atcaaatcaa attagccgat caaaaaatcg tccagatctt tgaagaaaat
      181 atcggtataa gtcttactag atacgagatc atgatgagac tgaaagaaga aaatccattg
      241 ctacaaagca gtttgcaaga aaagttgcag attgatcaag gagcagttac tcgtcatctg
      301 aaggtattgg aagcaaatgg ctatgtttcg cgtcagagaa atcctcagaa caaccgggaa
      361 gtgtatgtag cgctaacaga aaaagcccag aagaaaatca agcattgtga aatggaccat
      421 aaagatacag gttccgtgct atccccggct ttcagcgatg tagaaatgaa acaatttcta
      481 acacttttag ataaattgaa tacccaactt tgcaatataa caacagaaac gaaagaggtt
      541 aataataatg gaacaaaaaa atgatttttc aacaattatg actggtagaa gatccgttcg
      601 cgtatatgat gaaacataca aaatcccaca cgaagaaatg ctggaaatga tcaaagaagc
      661 gacgactgca ccttcttctg tcaatatgaa accatggcgc tttgtggtcg ctgaaagcga
      721 ggaagcaaaa gaaatcttgc gtcctttgat ccgtttcaat acgagacaaa atgatacttc
      781 ttctgcaatg gtcatgattt ttggcgatat gcaatgttac gaatatggcg aagaaatcta
      841 caaccaagct tacgaatcag ggaagatgcc aaaagaagtc agagatcaac aattagctgc
      901 gatcattcct tattacaaga gtttttcaag agaagaaatg aacgatgtag tcaaagtcga
      961 ttctagctta gcggctatgc aattcatgtt agttgcgcgt gatcatggct atgaaacaaa
     1021 tccaattggt ggattcgaag cagatcagtt ggcagaggca tttggtttgg ataaagaccg
     1081 ttatgttcca gttattattt tatcaatagg aaaagcggta gaagaaggtt acgagtctgt
     1141 tcgtttagca ccagaaaaga tcactatgtt cgaataaaag gttttcagtt actattcagt
     1201 agctattgct ttttttgaat cgtgtgtggt aacctaataa aaaaggaggt cggaaaaatg
     1261 actgcatcga tgaaattacg tttcgaactt ttgaataaca actaaataaa ttcaaaagac
     1321 atgtataaac aacaatttca tggatggagt atgtccattt tttcgaacga cagtagaata
     1381 ggtgaaagct atgacagatt tggtgattta gtcatagtta tttcgttcag agtaggaaaa
     1441 gatgtgatgc agtttttttg caaacagatt tttctgctct tttttagtat cgataattgt
     1501 tgttttacat gaaataggag tgtaaaacat atggaaaatt tagcagtaaa tataacaaat
     1561 ctacaagtca gctttggcaa ccagctagaa ctatctattg attctcttcg tgtctatcag
     1621 caagatcgta tagggatcat aggagaaaat ggtgtcggta aatcgacttt gctcaaacta
     1681 atagccggtg aactttttcc cgatcatggg aaaatccaaa cagagatcac cttcaactac
     1741 ctgcctcaat taacctatct cgctgaagca aaggacctaa atttggaatt agccagtcat
     1801 ttccagttaa gactggaaga aacttcagag cggaaatgga gcggagggga agaacgaaag
     1861 atcgagttga tacgtcttct ttcttcttat gaacaaggga tgcttctaga cgagccaaca
     1921 acccatctag atagaaaaag tattgatcga ctgattgaag agcttcgtta ttattatggt
     1981 acgctggttt ttgttagtca tgatcgctat tttctagatg agctagcatc gaaaatctgg
     2041 gaagtaaaag acggagaaat ccgagagttt tcggggaatt atagtgccta tctcactcaa
     2101 aaggaattgg agaaaaagac tcagctacga gaagcagagt cgatcatgaa agagaaaaaa
     2161 cgattggaaa aatcgatcca agaaaagaaa aaacaagcgg aaaagttgga aaaagtgtcc
     2221 agtaaaaaga aaaagcaaca aatcagactg gatcggttgt cttcctctaa acaaaaagac
     2281 agtgtacaaa aagccatcca aaagaatgcg aaaacattag agagaagact ccaaaaaata
     2341 ggagaaacaa ccaagccgca gcagatgaaa caaatccgtt ttccagtacc aaaatctctt
     2401 gaactccaca gccgctatcc catcatggga caaaatgtcc aattggaaag aagtgggaga
     2461 acattactgg taaatggtga ttttcagttt tctttaggta aaaaaatcgc gattgtcggt
     2521 gaaaatggtt caggtaagac aaccttatta gaacatatcc gcaaacaagg agaaggaatc
     2581 cttctctctc cgaaagtaag ctttcaagta tatcagcaaa agggttatca aatgacatct
     2641 gaagaatcca tcattcgttt tgtcatgaga caaacagagt tttcagaatc gcttgtccgt
     2701 agtttgctga atcacttagg gtttgctcag gaaactttga cgaaaccgtt atgtacatta
     2761 agtgggggag aagcgacccg tctgacgatt gctttgcttt ttactaagcc aagtaatgtg
     2821 ttgctgttag atgaaccgac taattttatt gatatggcaa cgatcgaagc tttagagaag
     2881 ctaatgcaaa tatatccggg aacgattttg tttacttctc atgattctta ctttgtcgag
     2941 cgtacggctg atgaagttta tgaaataaaa gggcagaaaa taaaaaaagt acttacgaga
     3001 aatttttaaa tgtgctacga tgattttcgt gacaaattta aaggagtggt acagattgcc
     3061 tacaaataaa aaagaaggaa taatttttac aacaatgatg tgttttttga tggtcttggg
     3121 gatgagcgcg tataatttat ggctgcatca agatcttcat ttatcaagtt tgttgattgg
     3181 tctagtgcct ggattcgtgg tggctttcat tttggatgtc ttcatcatcg gtgtgctggc
     3241 gaaaaaaatt gcctttaaac taccagtaaa tccagaaagc aaggtccaaa tgatcttaac
     3301 tatttcatgc ttgatggttt taggaatggt gacatgtatg tctttattcg gtgtgatcat
     3361 ggaacaagga atacctgatc aattattctc tgcttatatg aatgcttgga aaatgaatgt
     3421 gattgccgct ttaccgttgc agttgatcat cgttggcccg ctttcaagaa gtgtgctaaa
     3481 agcaattcaa agtaatgaat gatggaacac tgttatttga aaaaaggcct tggcatgctt
     3541 tttgtcatag tctcttgaat gcagttctag aaaaatagtg atccgaacga taggttttgg
     3601 cctgtcgttc ggatcgcttt ttcttttcca actgtattgc tcttttattg aagataattt
     3661 gctcttcaat agatatttct agaaaggttc tgtttatccc ttactcaaaa aactgctgca
     3721 tttgttctct tacttgctga caccattgga gttcctgctg acaaagcata attctgcgct
     3781 ggatcacaac ataacgtcca aattcttgtt tcttgtctag cgagaggtca ttcaattttt
     3841 tttgattttt ttgcatgatt ttgttcagct gttgttctct agtgaatagc agtccagtag
     3901 ctgttgtttt atcgagcgtc gaaaaagcat agatttttgc tagaaattca tctttttgcg
     3961 taggagcggg agtttcctct ttgatccatt cctctacaag agcacgtcct tcatcagtca
     4021 agtggtagat ctttttttga gagtgctcgt ctttgtccaa aacggtcaca taaccctgct
     4081 ggtctaactt ggcaagcgtt gtatagatct gactgtgatg ggcttcccaa aaaattgtga
     4141 tcagctgctt caattcatac ccagaacgtg gtttggataa aagcacgcat aataatatat
     4201 aggctaaagt attcataaaa a
//
LOCUS       DAJSBL010000217         3274 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.guided.135, whole genome shotgun sequence.
ACCESSION   DAJSBL010000217 DAJSBL010000000
VERSION     DAJSBL010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3274
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.guided.135"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            174..671
                     /gene="dfrG"
                     /locus_tag="ONT85_003069"
     CDS             174..671
                     /gene="dfrG"
                     /locus_tag="ONT85_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HCR2379942.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(743..2695)
                     /locus_tag="ONT85_003070"
     CDS             complement(743..2695)
                     /locus_tag="ONT85_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HCR2379943.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(2702..2938)
                     /locus_tag="ONT85_003071"
     CDS             complement(2702..2938)
                     /locus_tag="ONT85_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2379944.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            complement(3173..>3274)
                     /locus_tag="ONT85_003072"
     CDS             complement(3173..>3274)
                     /locus_tag="ONT85_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HCR2379945.1"
                     /translation="TNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAY"
BASE COUNT         1005 a          565 c          544 g         1160 t
ORIGIN      
        1 taaaatgcaa ttgaaaatga taattgaatg aaaatttgtg attttgtggt attatgataa
       61 tataacttat agaaaaaatt gcttattcaa ctaacgggca ggataatgag aaaaggtttt
      121 ttgatagaaa taagaaatta gatggtaaat tagttaaaaa ggctgtgtta taaatgaaag
      181 tttctttgat tgctgcgatg gataagaata gagtgattgg caaagagaat gacattcctt
      241 ggaggattcc caaggactgg gaatatgtta aaaatactac aaagggacat ccgataatat
      301 taggtaggaa gaaccttgaa tcaatcggaa gagccttacc tgacagaaga aatattattc
      361 tgacgagaga taaggggttt acctttaatg gttgtgaaat tgttcattca atagaagatg
      421 tttttgagtt atgtaaaaac gaagaagaaa tttttatttt cggaggagaa cagatttata
      481 atttgttttt cccttatgtt gagaaaatgt acatcacaaa aatacatcat gaattcgaag
      541 gagatacttt ttttccagaa gtgaattatg aggaatggaa tgaggtattt gcccaaaaag
      601 ggataaagaa tgataaaaat ccgtataact actattttca tgtatatgaa agaaaaaact
      661 tattgagtta acgggtgcgt taattgaaga agaacagtaa agataaagca caagagcttg
      721 ggattactcc aagctctttt tgtcatttga aataaattat atctttccaa tcaaattgtt
      781 tatctgtaat ccctaatctt tgagcaggtg tcaaaacctt tttatccgct gatttaaaag
      841 ggcgacaaaa gttgtagtaa gttcttagaa ttgtaatcgc aaattgagca tacttcggat
      901 tgaagttagc gtaaatatag ctcttcttat caccacgagc tgttgttaat ggtctttcaa
      961 gagatgatat ataacgtctt atctgttgaa taaagctatt ggtcgaatgg tcattaacat
     1021 ttaatagcat ctttgcgatt tcgtttggtt ccagagcaga taaatccgtt ctacaatcaa
     1081 ctgaataaaa tcctttatcc ttagtggcta atggatgttt gatagggtta tcggcatatt
     1141 tgcgatatct ttctccatct ttactaatag cctcttcgtg gaattgatga cttgttagta
     1201 attcagttag ataatgagat gccaaagtcc gtagaaactt tgttttgaat cctctgatat
     1261 ctccccaatc gagcagttct gcttttgctt gtccgaattc ttttaagcat tgttttctgc
     1321 ttttcgtctt gttaacttgg caaataaaat gatgtgcatc gctcaacctt aattctttcg
     1381 tgaatactct ataaaaggcg tttctaatag agaagtcatc atcactaatc attcgccatt
     1441 cgtcggcatt aaccatttgt ttaattaacc aatagtgagc agttgtagta taaggagcat
     1501 caacatttaa accatcgaca aatctactcc tattagtaat tttccctaat tcgtgaagat
     1561 aagcattcct attttcgcta tcgttagcgg atggttcttg tggataataa gaccaatcta
     1621 acctatcatt tttacgacta aaagtattga gatggtcttc tttgaatttt cttgtatctt
     1681 catttaattc atccattgaa atattccaat cataggcaac atcagaacgg aacacatatc
     1741 ttgataagac ctcagcagat acaactatat acgtttgcat attaaggtct tcaaaccccg
     1801 tatatttctt tgaaccttgt cctttcttac ggacgttatt taggtagtag tgcatcttgt
     1861 ccgtattaag ccacatctcg ttaaatttct ttgtttgaag gggttgtgtc tcatatcgtt
     1921 ctaaaaactc caaacaacgc ctatacagcc actccagctt atgatagtag gttccaactc
     1981 caattcctaa aatatcacaa gtacgactga ctgaaaccct tccaacaacc atcttagcga
     2041 acattggaag aatggtgttc ttttgttggt gataagtagt ggtttcttct ctttttggta
     2101 gaacattagt gaacttctta cacgccttac attgatagcg ttgagatttg cccctacttt
     2161 tccccctttt gtaaaattgc ttcggctcat tgaatggggt tgattcttct tccgagcagc
     2221 cttctttatg aaattgataa tctggctcaa catcttggat gctattgatt tctataaggc
     2281 gtttaatttc ttcaacaaca gaccaattag ataaaggggt tactgtacag ttttgcgata
     2341 cacctctgcc aataggattg tcattgcagt acataccttt atgaccttta tctttggaac
     2401 taccagtcat tttatatcga cttggtttcc cttttacatc tttgaatttg tgctgttctt
     2461 tcccaaaatt acaacaaaac ggattgatgc agtaattata ttgaatggga tattcagaac
     2521 cgttccattt aaaagacgtt ggcaagaaca acattttgtt ataccttgtt gcaaatcgtt
     2581 ttggagttaa taaatcatat tgtttagccc tatcttttaa ttcttcgttt gaaatcggaa
     2641 ttttaacatc tacaatttca tcttccttcg tggctaatct tttaagcttg gtcattgtct
     2701 atcaacccac tctatctttg atacccattt tttcaacaat ccgcttatta cttcgcttgt
     2761 tggcaatcca ttcaataaac ttcttatcgt ctaaaatgtt caataagaat tcatccactg
     2821 cttcgctttc agtacgctct gcatattcag cgtattgctt aactatcacc cttacttgct
     2881 ctgatatttc ccaatccaca gacttagcgt tcttattttt tggctcaaga aattgcatag
     2941 gaaaatgcac ccctttttgc ttagtaaatt gtaaccattt taacacacca atttgcattc
     3001 ctacaataat tatttcacgt tcgcattttt atatacattt taacttacat tttgtggtgt
     3061 aaattaacaa caaacgttga tttaacaata aaaaacgagt atcaaattca gttgcaattt
     3121 gatactcatt taggtttaca ttttcacttt ttgtactcca aaactgaact acttaatatg
     3181 caagacgttg tctttttttt gtatattcaa ttttgaaaac atttccgcaa agttcagacg
     3241 gacctcgatg tgttgatgaa taaaatttat tagt
//
LOCUS       DAJSBL010000218         2158 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.guided.155, whole genome shotgun sequence.
ACCESSION   DAJSBL010000218 DAJSBL010000000
VERSION     DAJSBL010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2158
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.guided.155"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            22..1941
                     /gene="tet(M)"
                     /locus_tag="ONT85_003073"
     CDS             22..1941
                     /gene="tet(M)"
                     /locus_tag="ONT85_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HCR2379946.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYTSGKLLEALELEQEESIRFHNCSLFPVYH
                     GSAKNNIGIDNLIEVITNKFYSSTHRGQSELCGKVFKIEYSEKRQRLAYIRLYSGVLH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLDSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIEIKEPTVIYMERPLKKAEYTIHIEVPPNPFWASIGLSVA
                     QLPLGSGVQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            2060..>2158
                     /locus_tag="ONT85_003074"
     CDS             2060..>2158
                     /locus_tag="ONT85_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HCR2379947.1"
                     /translation="MCPVCGNKTRLKIREDTELKKFPLYCPKCRQEN"
BASE COUNT          754 a          324 c          443 g          637 t
ORIGIN      
        1 ttttgaatgg aggaaaatca catgaaaatt attaatattg gagttttagc tcatgttgat
       61 gcaggaaaaa ctaccttaac agaaagctta ttatataaca gtggagcgat tacagaatta
      121 ggaagcgtgg acaaaggtac aacgaggacg gataatacgc ttttagaacg tcagagagga
      181 attacaattc agacaggaat aacctctttt cagtgggaaa atacgaaggt gaacatcata
      241 gacacgccag gacatatgga tttcttagca gaagtatatc gttcattatc agttttagat
      301 ggggcaattc tactgatttc tgcaaaagat ggcgtacaag cacaaactcg tatattattt
      361 catgcactta ggaaaatggg gattcccaca atctttttta tcaataagat tgaccaaaat
      421 ggaattgatt tatcaacggt ttatcaggat attaaagaga aactttctgc cgaaattgta
      481 atcaaacaga aggtagaact gtatcctaat atgtgtgtga cgaactttac cgaatctgaa
      541 caatgggata cggtaataga gggaaacgat gaccttttag agaaatatac gtctgggaaa
      601 ttattggaag cattagaact cgaacaagag gaaagcataa gatttcataa ttgttccctg
      661 ttccctgttt atcacggaag tgcaaaaaac aatataggga ttgataacct tatagaagtg
      721 attacgaata aattttattc atcaacacat cgaggtcagt ctgaactttg cggaaaagtt
      781 ttcaaaattg agtattcgga aaaaagacag cgtcttgcat atatacgtct ttatagtggc
      841 gtactgcatt tgcgagattc ggttagaata tcggaaaagg aaaaaataaa aattacagaa
      901 atgtatactt caataaatgg tgaattatgt aaaatcgata aggcttattc cggggaaatt
      961 gttattttgc aaaatgagtt tttgaagtta gatagtgttc ttggagatac aaaactattg
     1021 ccacagagaa aaaagattga aaatccgcac cctctactac aaacaactgt tgaaccgagt
     1081 aaacctgaac agagagaaat gttgcttgat gcccttttgg aaatctcaga tagtgatccg
     1141 cttctacgat attacgtgga ttctacgaca catgaaatta tactttcttt cttagggaaa
     1201 gtacaaatgg aagtgattag tgcactgttg caagaaaagt atcatgtgga gatagaaata
     1261 aaagagccta cagtcattta tatggaaaga ccgttaaaaa aagcagagta taccattcac
     1321 atcgaagttc caccgaatcc tttctgggct tccattggtc tatctgtagc acagcttcca
     1381 ttagggagcg gagtacagta tgagagctcg gtttctcttg gatacttaaa tcaatcgttt
     1441 caaaatgcag ttatggaggg gatacgctat ggctgtgaac aaggattgta tggttggaat
     1501 gtgacggact gtaaaatctg ttttaagtat ggcttatact atagccctgt tagtacccca
     1561 gcagattttc ggatgcttgc tcctattgta ttggaacaag tcttaaaaaa agctggaaca
     1621 gaattgttag agccatatct tagttttaaa atttatgcgc cacaggaata tctttcacga
     1681 gcatacaacg atgctcctaa atattgtgcg aacatcgtag acactcaatt gaaaaataat
     1741 gaggtcattc ttagtggaga aatccctgct cggtgtattc aagaatatcg tagtgattta
     1801 actttcttta caaatggacg tagtgtttgt ttaacagagt taaaagggta ccatgttact
     1861 accggtgaac ctgtttgcca gccccgtcgt ccaaatagtc ggatagataa agtacgatat
     1921 atgttcaata aaataactta gtgtatttta tgttgttata taaatatggt ttcttgttaa
     1981 ataagatgaa atatttttta ataaagattt gaattaaagt gtaaaggagg agatagttat
     2041 tataaactac aagtggatat tgtgtcctgt atgtggaaat aaaacacgat taaagataag
     2101 ggaagatact gaattaaaaa aattccccct ctattgtccg aaatgcagac aagaaaat
//
LOCUS       DAJSBL010000219         2158 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.guided.156, whole genome shotgun sequence.
ACCESSION   DAJSBL010000219 DAJSBL010000000
VERSION     DAJSBL010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2158
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.guided.156"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            22..1941
                     /gene="tet(M)"
                     /locus_tag="ONT85_003075"
     CDS             22..1941
                     /gene="tet(M)"
                     /locus_tag="ONT85_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HCR2379948.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYTSGKLLEALELEQEESIRFHNCSLFPVYH
                     GSAKNNIGIDNLIEVITNKFYSSTHRGQSELCGKVFKIEYSEKRQRLAYIRLYSGVLH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLDSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIEIKEPTVIYMERPLKKAEYTIHIEVPPNPFWASIGLSVA
                     QLPLGSGVQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            2060..>2158
                     /locus_tag="ONT85_003076"
     CDS             2060..>2158
                     /locus_tag="ONT85_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HCR2379949.1"
                     /translation="MCPVCGNKTRLKIREDTELKKFPLYCPKCRQEN"
BASE COUNT          753 a          324 c          444 g          637 t
ORIGIN      
        1 ttttgaatgg aggaaaatca catgaaaatt attaatattg gagttttagc tcatgttgat
       61 gcgggaaaaa ctaccttaac agaaagctta ttatataaca gtggagcgat tacagaatta
      121 ggaagcgtgg acaaaggtac aacgaggacg gataatacgc ttttagaacg tcagagagga
      181 attacaattc agacaggaat aacctctttt cagtgggaaa atacgaaggt gaacatcata
      241 gacacgccag gacatatgga tttcttagca gaagtatatc gttcattatc agttttagat
      301 ggggcaattc tactgatttc tgcaaaagat ggcgtacaag cacaaactcg tatattattt
      361 catgcactta ggaaaatggg gattcccaca atctttttta tcaataagat tgaccaaaat
      421 ggaattgatt tatcaacggt ttatcaggat attaaagaga aactttctgc cgaaattgta
      481 atcaaacaga aggtagaact gtatcctaat atgtgtgtga cgaactttac cgaatctgaa
      541 caatgggata cggtaataga gggaaacgat gaccttttag agaaatatac gtctgggaaa
      601 ttattggaag cattagaact cgaacaagag gaaagcataa gatttcataa ttgttccctg
      661 ttccctgttt atcacggaag tgcaaaaaac aatataggga ttgataacct tatagaagtg
      721 attacgaata aattttattc atcaacacat cgaggtcagt ctgaactttg cggaaaagtt
      781 ttcaaaattg agtattcgga aaaaagacag cgtcttgcat atatacgtct ttatagtggc
      841 gtactgcatt tgcgagattc ggttagaata tcggaaaagg aaaaaataaa aattacagaa
      901 atgtatactt caataaatgg tgaattatgt aaaatcgata aggcttattc cggggaaatt
      961 gttattttgc aaaatgagtt tttgaagtta gatagtgttc ttggagatac aaaactattg
     1021 ccacagagaa aaaagattga aaatccgcac cctctactac aaacaactgt tgaaccgagt
     1081 aaacctgaac agagagaaat gttgcttgat gcccttttgg aaatctcaga tagtgatccg
     1141 cttctacgat attacgtgga ttctacgaca catgaaatta tactttcttt cttagggaaa
     1201 gtacaaatgg aagtgattag tgcactgttg caagaaaagt atcatgtgga gatagaaata
     1261 aaagagccta cagtcattta tatggaaaga ccgttaaaaa aagcagagta taccattcac
     1321 atcgaagttc caccgaatcc tttctgggct tccattggtc tatctgtagc acagcttcca
     1381 ttagggagcg gagtacagta tgagagctcg gtttctcttg gatacttaaa tcaatcgttt
     1441 caaaatgcag ttatggaggg gatacgctat ggctgtgaac aaggattgta tggttggaat
     1501 gtgacggact gtaaaatctg ttttaagtat ggcttatact atagccctgt tagtacccca
     1561 gcagattttc ggatgcttgc tcctattgta ttggaacaag tcttaaaaaa agctggaaca
     1621 gaattgttag agccatatct tagttttaaa atttatgcgc cacaggaata tctttcacga
     1681 gcatacaacg atgctcctaa atattgtgcg aacatcgtag acactcaatt gaaaaataat
     1741 gaggtcattc ttagtggaga aatccctgct cggtgtattc aagaatatcg tagtgattta
     1801 actttcttta caaatggacg tagtgtttgt ttaacagagt taaaagggta ccatgttact
     1861 accggtgaac ctgtttgcca gccccgtcgt ccaaatagtc ggatagataa agtacgatat
     1921 atgttcaata aaataactta gtgtatttta tgttgttata taaatatggt ttcttgttaa
     1981 ataagatgaa atatttttta ataaagattt gaattaaagt gtaaaggagg agatagttat
     2041 tataaactac aagtggatat tgtgtcctgt atgtggaaat aaaacacgat taaagataag
     2101 ggaagatact gaattaaaaa aattccccct ctattgtccg aaatgcagac aagaaaat
//
LOCUS       DAJSBL010000220         1656 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.guided.163, whole genome shotgun sequence.
ACCESSION   DAJSBL010000220 DAJSBL010000000
VERSION     DAJSBL010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1656
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.guided.163"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            188..1228
                     /locus_tag="ONT85_003077"
     CDS             188..1228
                     /locus_tag="ONT85_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HCR2379950.1"
                     /translation="MYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLPQRK
                     KIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLGKVQ
                     MEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVSPLP
                     LGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYSPVS
                     TPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIVNTQ
                     LKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRLNSR
                     IDKVRYMFNKIT"
     gene            1326..1514
                     /locus_tag="ONT85_003078"
     CDS             1326..1514
                     /locus_tag="ONT85_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HCR2379951.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(1572..>1656)
                     /locus_tag="ONT85_003079"
     CDS             complement(1572..>1656)
                     /locus_tag="ONT85_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HCR2379952.1"
                     /translation="MDSFTDKELSLMEALAKGINEARNIEG"
BASE COUNT          557 a          252 c          311 g          536 t
ORIGIN      
        1 aaatcatttt gtatgtaatt tggtatgtaa gttaaaatga aaaaagtagt ccattttgaa
       61 gtacattttg tcattttgta ctccaaaact gaactactta cttgcatata tacgccttta
      121 tagtggagta ctacatttac gagattcggt tagagtatca gaaaaagaaa aaataaaagt
      181 tacagaaatg tatacttcaa taaatggtga attatgtaag attgatagag cttattctgg
      241 agaaattgtt attttgcaaa atgagttttt gaagttaaat agtgttcttg gagatacaaa
      301 actattgcca cagagaaaaa agattgaaaa tccgcaccct ctactacaaa caactgttga
      361 accgagtaaa cctgaacaga gagaaatgtt gcttgatgcc cttttggaaa tctcagatag
      421 tgatccgctt ctacgatatt acgtggattc tacgacacat gaaattatac tttctttctt
      481 agggaaagta caaatggaag tgattagtgc actgttgcaa gaaaagtatc atgtggagat
      541 agaactaaaa gagcctacag tcatttatat ggagagaccg ttaaaaaatg cagaatatac
      601 cattcacatc gaagtgccgc caaatccttt ctgggcttcc attggtttat ctgtatcacc
      661 gcttccgttg ggaagtggaa tgcagtatga gagctcggtt tctcttggat acttaaatca
      721 atcatttcaa aatgcagtta tggaagggat acgctatggt tgcgaacaag gattatatgg
      781 ttggaatgtg acggattgta aaatctgttt taagtacggt ttatactata gccctgttag
      841 tactccagca gattttcgga tgcttactcc tattgtactg gagcaagcct ttagaaaagc
      901 tggaacagaa ttgttagagc catatcttag ttttaaagtt tatgcaccac aggaatatct
      961 ttcacgggca tataacgatg ctcccaaata ttgtgcaaat atcgtaaata ctcaactgaa
     1021 aaataatgag gtcattatta ttggagaaat tcctgctcga tgtattcaag attatcgcaa
     1081 tgatttaact ttttttacaa atgggcttag tgtttgttta gcagagctaa aaggatatca
     1141 ggttaccact ggcgaacctg tttgccagac ccgtcgtcta aatagtcgga tagataaagt
     1201 aagatatatg ttcaataaaa taacttagtg cgttttatgt tgttatataa atatggtttc
     1261 ttattaaata agatgaaata ttctttaata tagatttgaa ttaaagtgga aaggaggaga
     1321 ttgttattat aaactacaag tggatattgt gtcctatttg tggaaataaa acaagactac
     1381 gaatacgagt ggatactata cttaaaaatt tccctttata ctgccccaaa tgtaagaacg
     1441 aaactttaat taatgttcaa aaaatgaata taataacaat caaagagcca gacgccaaga
     1501 cgcagagccg ataatttgag aaatgaaact ctcatcttat cggctctttt tgtttatctg
     1561 aattttactg actagccttc aatatttctt gcttcattga taccttttgc tagtgcttcc
     1621 attaaggata gttctttgtc tgtaaagcta tccatg
//
LOCUS       DAJSBL010000221         1602 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.guided.165, whole genome shotgun sequence.
ACCESSION   DAJSBL010000221 DAJSBL010000000
VERSION     DAJSBL010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1602
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.guided.165"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            24..416
                     /locus_tag="ONT85_003080"
     CDS             24..416
                     /locus_tag="ONT85_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR2379953.1"
                     /translation="MKLLFIDDNMVFDIDNLKGFLNDTSSFGFIAKENNKIIGFAYCY
                     TLLRPDGKTMFYLHSIGMLPNYQDKGYGSKLLSFIKEYSKEIGCSEMFLITDKGNPRA
                     CHVYEKLGGKNDYKDEIVYVYDYEKGDK"
     gene            417..>1602
                     /gene="aac(6')-Ie"
                     /locus_tag="ONT85_003081"
     CDS             417..>1602
                     /gene="aac(6')-Ie"
                     /locus_tag="ONT85_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ie"
                     /protein_id="HCR2379954.1"
                     /translation="MNIVENEICIRTLIDDDFPLMLKWLTDERVLEFYDGRDKKYTLE
                     SLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHYPKTDEIVYGMDQFI
                     GEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIRAYQKSGFRIIEDLP
                     EHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDSIEIIGSGYDSVAYL
                     VNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIEYSYISDELSILGYKE
                     IKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISECTIDNKQNVLEEYIL
                     LRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSCNHLLLDGNNRLTGII
                     DFG"
BASE COUNT          654 a          127 c          270 g          551 t
ORIGIN      
        1 tatagttgaa gaatattatc ctattgaaac tattgtttat tgatgataat atggtttttg
       61 atattgataa tttaaaagga tttcttaatg ataccagttc atttgggttt atagctaaag
      121 aaaataataa aattatagga tttgcatatt gctatacact tttaagacct gatggaaaaa
      181 caatgtttta tttacactca ataggaatgt tacctaacta tcaagacaaa ggttatggtt
      241 caaaattatt atcttttatt aaggaatatt ctaaagagat tggttgttct gaaatgtttt
      301 taataactga taaaggtaat cctagagctt gccatgtata tgaaaaatta ggtggtaaaa
      361 atgattataa agatgaaata gtatatgtat atgattatga aaaaggtgat aaataaatga
      421 atatagttga aaatgaaata tgtataagaa ctttaataga tgatgatttt cctttgatgt
      481 taaaatggtt aactgatgaa agagtattag aattttatga tggtagagat aaaaaatata
      541 cattagaatc attaaaaaaa cattatacag agccttggga agatgaagtt tttagagtaa
      601 ttattgaata taacaatgtt cctattggat atggacaaat atataaaatg tatgatgagt
      661 tatatactga ttatcattat ccaaaaactg atgagatagt ctatggtatg gatcaattta
      721 taggagagcc aaattattgg agtaaaggaa ttggtacaag atatattaaa ttgatttttg
      781 aatttttgaa aaaagaaaga aatgctaatg cagttatttt agaccctcat aaaaataatc
      841 caagagcaat aagggcatac caaaaatctg gttttagaat tattgaagat ttgccagaac
      901 atgaattaca cgagggcaaa aaagaagatt gttatttaat ggaatataga tatgatgata
      961 atgccacaaa tgttaaggca atgaaatatt taattgagca ttactttgat aatttcaaag
     1021 tagatagtat tgaaataatc ggtagtggtt atgatagtgt ggcatattta gttaataatg
     1081 aatacatttt taaaacaaaa tttagtacta ataagaaaaa aggttatgca aaagaaaaag
     1141 caatatataa ttttttaaat acaaatttag aaactaatgt aaaaattcct aatattgaat
     1201 attcgtatat tagtgatgaa ttatctatac taggttataa agaaattaaa ggaacttttt
     1261 taacaccaga aatttattct actatgtcag aagaagaaca aaatttgtta aaacgagata
     1321 ttgccagttt tttaagacaa atgcacggtt tagattatac agatattagt gaatgtacta
     1381 ttgataataa acaaaatgta ttagaagagt atatattgtt gcgtgaaact atttataatg
     1441 atttaactga tatagaaaaa gattatatag aaagttttat ggaaagacta aatgcaacaa
     1501 cagtttttga gggtaaaaag tgtttatgcc ataatgattt tagttgtaat catctattgt
     1561 tagatggcaa taatagatta actggaataa ttgattttgg ag
//
LOCUS       DAJSBL010000222         1504 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.guided.168, whole genome shotgun sequence.
ACCESSION   DAJSBL010000222 DAJSBL010000000
VERSION     DAJSBL010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1504
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.guided.168"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            262..345
                     /locus_tag="ONT85_003082"
                     /pseudo
     CDS             262..345
                     /locus_tag="ONT85_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527320.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
     gene            394..477
                     /locus_tag="ONT85_003083"
     CDS             394..477
                     /locus_tag="ONT85_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuator leader
                     peptide ErmL"
                     /protein_id="HCR2379955.1"
                     /translation="MLVFQMRYVDKTSTVLKQTKNSDYADK"
     gene            602..1339
                     /gene="erm(B)"
                     /locus_tag="ONT85_003084"
     CDS             602..1339
                     /gene="erm(B)"
                     /locus_tag="ONT85_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HCR2379956.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
BASE COUNT          551 a          224 c          245 g          484 t
ORIGIN      
        1 atttgtatcg ataagaaata gatttaaaaa tttcgctgtt attttgtaca tttaacttga
       61 cggtgacatc tctctattgt gagttattag tggtacagtt ttcaaccgtt ttaattataa
      121 aaaagtggtg catttttaaa ttggcacaaa caggtaacgg ttattgcagg tgtatttctt
      181 atctatgggt ttaacatgga ttttatcatt aaaatcatga gtattgtccg agagtgattg
      241 gtcttgcgta tggttaaccc taaagttatg gaaataagac ttagaagcaa acttaagagt
      301 gtgttgatag tgcattatct taaaattttg tataatagga attgaagtta aattagatgc
      361 taaaaatttg taattaagaa ggagggattc gtcatgttgg tattccaaat gcgttatgta
      421 gataaaacat ctactgtttt gaaacagact aaaaacagtg attacgcaga taaataaata
      481 cgttagatta attcctacca gtgactaatc ttatgacttt ttaaacagat aactaaaatt
      541 acaaacaaat cgtttaactt ctgtatttgt ttatagatgt aatcacttca ggagtgataa
      601 catgaacaaa aatataaaat attctcaaaa ctttttaacg agtgaaaaag tactcaacca
      661 aataataaaa caattgaatt taaaagaaac cgataccgtt tacgaaattg gaacaggtaa
      721 agggcattta acgacgaaac tggctaaaat aagtaaacag gtaacgtcta ttgaattaga
      781 cagtcatcta ttcaacttat cgtcagaaaa attaaaactg aatactcgtg tcactttaat
      841 tcaccaagat attctacagt ttcaattccc taacaaacag aggtataaaa ttgttgggaa
      901 tattccttac catttaagca cacaaattat taaaaaagtg gtttttgaaa gccgtgcgtc
      961 tgacatctat ctgattgttg aagaaggatt ctacaagcgt accttggata ttcaccgaac
     1021 actagggttg ctcttgcaca ctcaagtctc gattcagcaa ttgcttaagc tgccagcgga
     1081 atgctttcat cctaaaccaa aagtaaacag tgtcttaata aaacttaccc gccataccac
     1141 agatgttcca gataaatatt ggaagctata tacgtacttt gtttcaaaat gggtcaatcg
     1201 agaatatcgt caactgttta ctaaaaatca gtttcatcaa gcaatgaaac acgccaaagt
     1261 aaacaattta agtaccgtta cttatgagca agtattgtct atttttaata gttatctatt
     1321 atttaacggg aggaaataat tctatgagtc gcttttgtaa atttggaaag ttacacgtta
     1381 ctaaagggaa tgtagataaa ttattaggta tagggcacct ctaataacta ccaattaatt
     1441 cacctcaaaa tgaaattaga aaaaaacagc gaaattttta aatctatttc ttatcgatac
     1501 aaat
//
LOCUS       DAJSBL010000223         1382 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.guided.176, whole genome shotgun sequence.
ACCESSION   DAJSBL010000223 DAJSBL010000000
VERSION     DAJSBL010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1382
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.guided.176"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..1311
                     /locus_tag="ONT85_003085"
     CDS             <1..1311
                     /locus_tag="ONT85_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aminoglycoside N-acetyltransferase
                     AAC(6')-Ie/aminoglycoside O-phosphotransferase
                     APH(2'')-Ia"
                     /protein_id="HCR2379957.1"
                     /translation="ESLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYH
                     YPKTDEIVYGMDQFIGEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAI
                     RAYQKSGFRIIEDLPEHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVD
                     SIEIIGSGYDSVAYLVNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIE
                     YSYISDELSILGYKEIKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISE
                     CTIDNKQNVLEEYILLRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSC
                     NHLLLDGNNRLTGIIDFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDIE
                     KAKEYQDIVEEYYPIETIVYGIKNIKQEFIENGRKEIYKRTYKD"
BASE COUNT          584 a          108 c          234 g          456 t
ORIGIN      
        1 gaatcattaa aaaaacatta tacagagcct tgggaagatg aagtttttag agtaattatt
       61 gaatataaca atgttcctat tggatatgga caaatatata aaatgtatga tgagttatat
      121 actgattatc attatccaaa aactgatgag atagtctatg gtatggatca atttatagga
      181 gagccaaatt attggagtaa aggaattggt acaagatata ttaaattgat ttttgaattt
      241 ttgaaaaaag aaagaaatgc taatgcagtt attttagacc ctcataaaaa taatccaaga
      301 gcaataaggg cataccaaaa atctggtttt agaattattg aagatttgcc agaacatgaa
      361 ttacacgagg gcaaaaaaga agattgttat ttaatggaat atagatatga tgataatgcc
      421 acaaatgtta aggcaatgaa atatttaatt gagcattact ttgataattt caaagtagat
      481 agtattgaaa taatcggtag tggttatgat agtgtggcat atttagttaa taatgaatac
      541 atttttaaaa caaaatttag tactaataag aaaaaaggtt atgcaaaaga aaaagcaata
      601 tataattttt taaatacaaa tttagaaact aatgtaaaaa ttcctaatat tgaatattcg
      661 tatattagtg atgaattatc tatactaggt tataaagaaa ttaaaggaac ttttttaaca
      721 ccagaaattt attctactat gtcagaagaa gaacaaaatt tgttaaaacg agatattgcc
      781 agttttttaa gacaaatgca cggtttagat tatacagata ttagtgaatg tactattgat
      841 aataaacaaa atgtattaga agagtatata ttgttgcgtg aaactattta taatgattta
      901 actgatatag aaaaagatta tatagaaagt tttatggaaa gactaaatgc aacaacagtt
      961 tttgagggta aaaagtgttt atgccataat gattttagtt gtaatcatct attgttagat
     1021 ggcaataata gattaactgg aataattgat tttggagatt ctggaattat agatgaatat
     1081 tgtgatttta tatacttact tgaagatagt gaagaagaaa taggaacaaa ttttggagaa
     1141 gatatattaa gaatgtatgg aaatatagat attgagaaag caaaagaata tcaagatata
     1201 gttgaagaat attatcctat tgaaactatt gtttatggaa ttaaaaatat taaacaggaa
     1261 tttatcgaaa atggtagaaa agaaatttat aaaaggactt ataaagattg attatataat
     1321 atatgaaaag ctattataaa agacggttct gttgcaaagt tttaaatcta ctatcaaata
     1381 ag
//
LOCUS       DAJSBL010000224         1350 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.guided.178, whole genome shotgun sequence.
ACCESSION   DAJSBL010000224 DAJSBL010000000
VERSION     DAJSBL010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1350
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.guided.178"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            47..298
                     /locus_tag="ONT85_003086"
                     /pseudo
     CDS             47..298
                     /locus_tag="ONT85_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            391..1185
                     /locus_tag="ONT85_003087"
                     /note="aph(3')-IIIa"
     CDS             391..1185
                     /locus_tag="ONT85_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HCR2379958.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
BASE COUNT          425 a          234 c          322 g          369 t
ORIGIN      
        1 acattttcct ctaggaaaaa attaactagc accacgggtt atcttttata tagaagatat
       61 cgccgtatgt aaggatttca gggggcaagg cataggcagc gcgcttatca atatatctat
      121 agaatgggca aagcataaaa acttgcatgg actaatgctt gaaacccagg acaataacct
      181 tatagcttgt aaattctatc ataattgtgg tttcaaaatc ggctccgtcg atactatgtt
      241 atacgccaac tttgaaaaca actttgaaaa agctgttttc tggtatttaa ggttttagaa
      301 tgcaaggaac agtgaattgg agttcgtctt gttataatta gcttcttggg gtatctttaa
      361 atactgtaga aaagaggaag gaaataataa atggctaaaa tgagaatatc accggaattg
      421 aaaaaactga tcgaaaaata ccgctgcgta aaagatacgg aaggaatgtc tcctgctaag
      481 gtatataagc tggtgggaga aaatgaaaac ctatatttaa aaatgacgga cagccggtat
      541 aaagggacca cctatgatgt ggaacgggaa aaggacatga tgctatggct ggaaggaaag
      601 ctgcctgttc caaaggtcct gcactttgaa cggcatgatg gctggagcaa tctgctcatg
      661 agtgaggccg atggcgtcct ttgctcggaa gagtatgaag atgaacaaag ccctgaaaag
      721 attatcgagc tgtatgcgga gtgcatcagg ctctttcact ccatcgacat atcggattgt
      781 ccctatacga atagcttaga cagccgctta gccgaattgg attacttact gaataacgat
      841 ctggccgatg tggattgcga aaactgggaa gaagacactc catttaaaga tccgcgcgag
      901 ctgtatgatt ttttaaagac ggaaaagccc gaagaggaac ttgtcttttc ccacggcgac
      961 ctgggagaca gcaacatctt tgtgaaagat ggcaaagtaa gtggctttat tgatcttggg
     1021 agaagcggca gggcggacaa gtggtatgac attgccttct gcgtccggtc gatcagggag
     1081 gatatcgggg aagaacagta tgtcgagcta ttttttgact tactggggat caagcctgat
     1141 tgggagaaaa taaaatatta tattttactg gatgaattgt tttagtacct agatttagat
     1201 gtctaaaaag ctttaactac aagcttttta gacatctaat cttttctgaa gtacatccgc
     1261 aactgtccat actctgatgt tttatatctt ttctaaaagt tcgctagata ggggtcccga
     1321 gcgcctacga ggaatttgta tcgataagaa
//
LOCUS       DAJSBL010000225          722 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-02
            SAMN30692145-rid15768473.guided.201, whole genome shotgun sequence.
ACCESSION   DAJSBL010000225 DAJSBL010000000
VERSION     DAJSBL010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692145
            Sequence Read Archive: SRR21444933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472592.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:27:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 21 of 75
            Pseudo Genes (incomplete)         :: 42 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 5 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..722
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692145-rid15768473.guided.201"
                     /isolate="VRED13-02"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            107..655
                     /gene="aac(6')"
                     /locus_tag="ONT85_003088"
     CDS             107..655
                     /gene="aac(6')"
                     /locus_tag="ONT85_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HCR2379959.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT          262 a          111 c          164 g          185 t
ORIGIN      
        1 taatgaaaga atagagatac tgattggtag ctaaagattt ttgagcgaga acatttcgtt
       61 ttttcaaaag gactgttaga ataagcaaga acaaggggga agacgtatga taatcagtga
      121 atttgaccgt aataatccag tattgaaaga tcagctttct gatttactga gactgacttg
      181 gccggaagaa tatggagaca gctcggcaga agaagtagaa gaaatgatga atccagaacg
      241 aatcgcggta gcagcggtag accaagatga gttagtagga tttattggtg caatccctca
      301 atacggtatc acaggttggg aattgcatcc attagttgta gaaagctccc gacgaaagaa
      361 ccaaataggt actcgattag tcaattactt agaaaaagaa gtagcttcca gaggaggaat
      421 cacgatttat ttaggtacgg atgatttaga ccatggaaca acgttaagtc aaacggacct
      481 gtatgagcat acatttgata aagtggcttc catccagaac cttcgtgaac atccgtatga
      541 attctatgaa aaattaggtt ataaaatcgt aggtgtctta ccaaatgcaa atggctggga
      601 caaaccggat atttggatgg caaaaacgat tattcctcga ccaaattctc aataataaaa
      661 agaggttgtg acagaagtaa tcagcttcaa gaaataagaa ggaatttacg aaaattactt
      721 ct
//
